pck cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	pck
Entrez Gene ID	31101
Full Name	pickel
Synonyms	CG14779,Dmel\CG14779,EG:80H7.2,Mega,PCK,Pck,l(1)G0044,mega
General protein information	Preferred Names pickel Names CG14779 gene product from transcript CG14779-RA CG14779-PA CG14779-PC CG14779-PD megatrachea pck-PA pck-PC pck-PD
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 2A4-2A4

mRNA and Protein(s)

mRNA	Protein	Name
NM_166887.3	NP_726742.1	pickel, isoform A
NM_130563.4	NP_569919.2	pickel, isoform C
NM_001038731.1	NP_001033820.1	pickel, isoform D

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	pck	NP_569919.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003808	XP_310368.7

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Proteomic identification of a set of 142 proteins that potentially interact with the claudin Mega.
Title: The claudin Megatrachea protein complex.

This paper identified Megatrachea, a Drosophila transmembrane protein homologous to claudins, and show that it acts in septate junctions.Also revealed that Mega has transepithelial barrier function similar to the claudins.
Title: The claudin-like megatrachea is essential in septate junctions for the epithelial barrier function in Drosophila.

The following pck gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the pck cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa00476	NM_166887.3 Latest version!	Drosophila melanogaster pickel (pck), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OFa00476	NM_130563.4 Latest version!	Drosophila melanogaster pickel (pck), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OFa00477	NM_001038731.1 Latest version!	Drosophila melanogaster pickel (pck), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

pck ( NM_166887.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_726742.1 Drosophila melanogaster pickel (pck), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa00476
Clone ID Related Accession (Same CDS sequence)	NM_166887.3 , NM_130563.4
Accession Version	NM_166887.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 771bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	pickel, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_166887.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCGCGAAC TTAACAAGCA GCAGTCGCAG GACACCACGG ACTCGGGCGT CGAAAAGGGC 
GACTATCCGC GGGCCACCAA TGGCGTGGTC TTCGGCGCCA TAGTTACATT CGCCAGCTTT 
TTCGTGCTGA TGATGTCCTT TTGCTCGCCG TACTGGATCG AGTCCTACGA GGAGACGCGG 
GCCAGCTTCA AGAACATGGG CCTGTGGCAG TACTGCTTTA AGGACTTCGT GTACCCCAAG 
TACGCGTTCT TGAAGCAGTT CACCGGTTGC CACAACATCT TCTCGCACGA GTATTACGTT 
ATCCGTGAGT ATCTGCTGCC CGGCTGGCTC ATGGCGGTGC AGGGCTTCGT GACCATGTCC 
TTCATCATCG TGTTCCTTGT GCTGGCCCTG CTCTCGCTTA CCATCATCCG ACTGCCTCTT 
AAGGCCGTTC TGCAGTACGA GTGGCTGCTC GTCCGCCTCT CCTACATGGG CACCGCCATC 
TCGTCTCTGT TCATGTTTTT GGCCGTATGC ATCTTCGGGG GCTGCGCCTA CCGCCGCGAC 
TGGATGATGT ACCCCAAGTT CAACGTGCTG GGCTGGTCCT ACGCCCTGGC CGTTGTCACC 
TTCATGCTCC TTGGTCTAGC CGCTCTGATC CTGCAGCGCG AGGCCCGGCA AGCCTACGAC 
GCCCGCGGCG AGCAGAAGAA CCTGGTAATG CAAATGGAGA TGCAGGAGCC GGGTTACCAG 
CCGCCGCGCC ACCACCACAG CCAGTCGCGG AGCCTGCAGG GCTACATATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_726742.1
CDS	248..1018
Translation	MRELNKQQSQDTTDSGVEKGDYPRATNGVVFGAIVTFASFFVLMMSFCSPYWIESYEETRASFKNMGLWQYCFKDFVYPKYAFLKQFTGCHNIFSHEYYVIREYLLPGWLMAVQGFVTMSFIIVFLVLALLSLTIIRLPLKAVLQYEWLLVRLSYMGTAISSLFMFLAVCIFGGCAYRRDWMMYPKFNVLGWSYALAVVTFMLLGLAALILQREARQAYDARGEQKNLVMQMEMQEPGYQPPRHHHSQSRSLQGYI

Target ORF information:

RefSeq Version	NM_166887.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster pickel (pck), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_166887.3

ORF Insert Sequence:

ATGCGCGAAC TTAACAAGCA GCAGTCGCAG GACACCACGG ACTCGGGCGT CGAAAAGGGC 
GACTATCCGC GGGCCACCAA TGGCGTGGTC TTCGGCGCCA TAGTTACATT CGCCAGCTTT 
TTCGTGCTGA TGATGTCCTT TTGCTCGCCG TACTGGATCG AGTCCTACGA GGAGACGCGG 
GCCAGCTTCA AGAACATGGG CCTGTGGCAG TACTGCTTTA AGGACTTCGT GTACCCCAAG 
TACGCGTTCT TGAAGCAGTT CACCGGTTGC CACAACATCT TCTCGCACGA GTATTACGTT 
ATCCGTGAGT ATCTGCTGCC CGGCTGGCTC ATGGCGGTGC AGGGCTTCGT GACCATGTCC 
TTCATCATCG TGTTCCTTGT GCTGGCCCTG CTCTCGCTTA CCATCATCCG ACTGCCTCTT 
AAGGCCGTTC TGCAGTACGA GTGGCTGCTC GTCCGCCTCT CCTACATGGG CACCGCCATC 
TCGTCTCTGT TCATGTTTTT GGCCGTATGC ATCTTCGGGG GCTGCGCCTA CCGCCGCGAC 
TGGATGATGT ACCCCAAGTT CAACGTGCTG GGCTGGTCCT ACGCCCTGGC CGTTGTCACC 
TTCATGCTCC TTGGTCTAGC CGCTCTGATC CTGCAGCGCG AGGCCCGGCA AGCCTACGAC 
GCCCGCGGCG AGCAGAAGAA CCTGGTAATG CAAATGGAGA TGCAGGAGCC GGGTTACCAG 
CCGCCGCGCC ACCACCACAG CCAGTCGCGG AGCCTGCAGG GCTACATATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pck ( NM_130563.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_569919.2 Drosophila melanogaster pickel (pck), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa00476
Clone ID Related Accession (Same CDS sequence)	NM_166887.3 , NM_130563.4
Accession Version	NM_130563.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 771bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	pickel, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_130563.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCGCGAAC TTAACAAGCA GCAGTCGCAG GACACCACGG ACTCGGGCGT CGAAAAGGGC 
GACTATCCGC GGGCCACCAA TGGCGTGGTC TTCGGCGCCA TAGTTACATT CGCCAGCTTT 
TTCGTGCTGA TGATGTCCTT TTGCTCGCCG TACTGGATCG AGTCCTACGA GGAGACGCGG 
GCCAGCTTCA AGAACATGGG CCTGTGGCAG TACTGCTTTA AGGACTTCGT GTACCCCAAG 
TACGCGTTCT TGAAGCAGTT CACCGGTTGC CACAACATCT TCTCGCACGA GTATTACGTT 
ATCCGTGAGT ATCTGCTGCC CGGCTGGCTC ATGGCGGTGC AGGGCTTCGT GACCATGTCC 
TTCATCATCG TGTTCCTTGT GCTGGCCCTG CTCTCGCTTA CCATCATCCG ACTGCCTCTT 
AAGGCCGTTC TGCAGTACGA GTGGCTGCTC GTCCGCCTCT CCTACATGGG CACCGCCATC 
TCGTCTCTGT TCATGTTTTT GGCCGTATGC ATCTTCGGGG GCTGCGCCTA CCGCCGCGAC 
TGGATGATGT ACCCCAAGTT CAACGTGCTG GGCTGGTCCT ACGCCCTGGC CGTTGTCACC 
TTCATGCTCC TTGGTCTAGC CGCTCTGATC CTGCAGCGCG AGGCCCGGCA AGCCTACGAC 
GCCCGCGGCG AGCAGAAGAA CCTGGTAATG CAAATGGAGA TGCAGGAGCC GGGTTACCAG 
CCGCCGCGCC ACCACCACAG CCAGTCGCGG AGCCTGCAGG GCTACATATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_569919.2
CDS	138..908
Translation	MRELNKQQSQDTTDSGVEKGDYPRATNGVVFGAIVTFASFFVLMMSFCSPYWIESYEETRASFKNMGLWQYCFKDFVYPKYAFLKQFTGCHNIFSHEYYVIREYLLPGWLMAVQGFVTMSFIIVFLVLALLSLTIIRLPLKAVLQYEWLLVRLSYMGTAISSLFMFLAVCIFGGCAYRRDWMMYPKFNVLGWSYALAVVTFMLLGLAALILQREARQAYDARGEQKNLVMQMEMQEPGYQPPRHHHSQSRSLQGYI

Target ORF information:

RefSeq Version	NM_130563.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster pickel (pck), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_130563.4

ORF Insert Sequence:

ATGCGCGAAC TTAACAAGCA GCAGTCGCAG GACACCACGG ACTCGGGCGT CGAAAAGGGC 
GACTATCCGC GGGCCACCAA TGGCGTGGTC TTCGGCGCCA TAGTTACATT CGCCAGCTTT 
TTCGTGCTGA TGATGTCCTT TTGCTCGCCG TACTGGATCG AGTCCTACGA GGAGACGCGG 
GCCAGCTTCA AGAACATGGG CCTGTGGCAG TACTGCTTTA AGGACTTCGT GTACCCCAAG 
TACGCGTTCT TGAAGCAGTT CACCGGTTGC CACAACATCT TCTCGCACGA GTATTACGTT 
ATCCGTGAGT ATCTGCTGCC CGGCTGGCTC ATGGCGGTGC AGGGCTTCGT GACCATGTCC 
TTCATCATCG TGTTCCTTGT GCTGGCCCTG CTCTCGCTTA CCATCATCCG ACTGCCTCTT 
AAGGCCGTTC TGCAGTACGA GTGGCTGCTC GTCCGCCTCT CCTACATGGG CACCGCCATC 
TCGTCTCTGT TCATGTTTTT GGCCGTATGC ATCTTCGGGG GCTGCGCCTA CCGCCGCGAC 
TGGATGATGT ACCCCAAGTT CAACGTGCTG GGCTGGTCCT ACGCCCTGGC CGTTGTCACC 
TTCATGCTCC TTGGTCTAGC CGCTCTGATC CTGCAGCGCG AGGCCCGGCA AGCCTACGAC 
GCCCGCGGCG AGCAGAAGAA CCTGGTAATG CAAATGGAGA TGCAGGAGCC GGGTTACCAG 
CCGCCGCGCC ACCACCACAG CCAGTCGCGG AGCCTGCAGG GCTACATATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pck ( NM_001038731.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001033820.1 Drosophila melanogaster pickel (pck), transcript variant D, mRNA.

Price & Availability↑

CloneID	OFa00477
Clone ID Related Accession (Same CDS sequence)	NM_001038731.1
Accession Version	NM_001038731.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 411bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	pickel, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCGCGAAC TTAACAAGCA GCAGTCGCAG GACACCACGG ACTCGGGCGT CGAAAAGGGC 
GACTATCCGC GGGCCACCAA TGGCGTGGTC TTCGGCGCCA TAGTTACATT CGCCAGCTTT 
TTCGTGCTGA TGATGTCCTT TTGCTCGCCG TACTGGATCG AGTCCTACGA GGAGACGCGG 
GCCAGCTTCA AGAACATGGG CCTGTGGCAG TACTGCTTTA AGGACTTCGT GTACCCCAAG 
TACGCGTTCT TGAAGCAGTT CACCGGTTGC CACAACATCT TCTCGCACGT GAGTACAGCA 
AACTGGCCGG TTCTTATCCT TGTCCTGATC TTCTCCCACT CGTACAGGAG TATTACGTTA 
TCCGTGAGTA TCTGCTGCCC GGCTGGCTCA TGGCGGTGCA GGGCTTCGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001033820.1
CDS	158..568
Translation	MRELNKQQSQDTTDSGVEKGDYPRATNGVVFGAIVTFASFFVLMMSFCSPYWIESYEETRASFKNMGLWQYCFKDFVYPKYAFLKQFTGCHNIFSHVSTANWPVLILVLIFSHSYRSITLSVSICCPAGSWRCRAS

Target ORF information:

RefSeq Version	NM_001038731.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster pickel (pck), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001038731.1

ORF Insert Sequence:

ATGCGCGAAC TTAACAAGCA GCAGTCGCAG GACACCACGG ACTCGGGCGT CGAAAAGGGC 
GACTATCCGC GGGCCACCAA TGGCGTGGTC TTCGGCGCCA TAGTTACATT CGCCAGCTTT 
TTCGTGCTGA TGATGTCCTT TTGCTCGCCG TACTGGATCG AGTCCTACGA GGAGACGCGG 
GCCAGCTTCA AGAACATGGG CCTGTGGCAG TACTGCTTTA AGGACTTCGT GTACCCCAAG 
TACGCGTTCT TGAAGCAGTT CACCGGTTGC CACAACATCT TCTCGCACGT GAGTACAGCA 
AACTGGCCGG TTCTTATCCT TGTCCTGATC TTCTCCCACT CGTACAGGAG TATTACGTTA 
TCCGTGAGTA TCTGCTGCCC GGCTGGCTCA TGGCGGTGCA GGGCTTCGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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pck cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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