Ns3 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ns3
Entrez Gene ID	31097
Full Name	Nucleostemin 3
Synonyms	A60,BACN32G11.5,CG14788,Dmel\CG14788,EG:BACN32G11.5,dNS3,dmNuc3,l(1)2Ad,l(1)BA11,l(1)G0431,ns3
General protein information	Preferred Names Nucleostemin 3 Names CG14788 gene product from transcript CG14788-RB CG14788-PA CG14788-PB Ns3-PA Ns3-PB lethal (1) 2Ad lethal (1) G0431 nucleostemin 3 nucleostemin ortholog 3
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 2B1-2A4

mRNA and Protein(s)

mRNA	Protein	Name
NM_001297850.1	NP_001284779.1	nucleostemin 3, isoform B
NM_130559.3	NP_569915.1	nucleostemin 3, isoform A

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	LSG1	XP_516958.2
Bos taurus (cattle)	LSG1	NP_001039375.1
Mus musculus (house mouse)	Lsg1	NP_835170.1
Gallus gallus (chicken)	LSG1	NP_001006549.1
Saccharomyces cerevisiae (baker's yeast)	LSG1	NP_011416.3
Kluyveromyces lactis	KLLA0F06666g	XP_455382.1
Schizosaccharomyces pombe (fission yeast)	SPAC3F10.16c	NP_593948.1
Rattus norvegicus (Norway rat)	Lsg1	NP_001013439.1
Danio rerio (zebrafish)	lsg1	NP_997807.1
Drosophila melanogaster (fruit fly)	ns3	NP_569915.1
Neurospora crassa	NCU04166	XP_960724.1
Oryza sativa (rice)	Os12g0618300	NP_001067291.1
Xenopus tropicalis (tropical clawed frog)	lsg1	XP_002937880.1
Caenorhabditis elegans (roundworm)	C53H9.2	NP_740787.2
Eremothecium gossypii	AGOS_AFL042C	NP_985506.2
Arabidopsis thaliana (thale cress)	AT1G08410	NP_172317.1
Arabidopsis thaliana (thale cress)	AT2G27200	NP_180288.1
Homo sapiens (human)	LSG1	NP_060855.2
Canis lupus familiaris (dog)	LSG1	XP_005639715.1
	LOC708114	XP_001097108.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011471	XP_317835.4
Magnaporthe oryzae (rice blast fungus)	MGG_07525	XP_003711438.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The ribosome biogenesis role of NS3 is essential for proper rates of translation in all tissues and is necessary for functions of growth-promoting neurons.
Title: Regulation of ribosome biogenesis by nucleostemin 3 promotes local and systemic growth in Drosophila.

The following Ns3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ns3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa00472	NM_001297850.1 Latest version!	Drosophila melanogaster nucleostemin 3, transcript variant B (Ns3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa00472	NM_130559.3 Latest version!	Drosophila melanogaster nucleostemin 3, transcript variant A (Ns3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ns3 ( NM_001297850.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001284779.1 Drosophila melanogaster nucleostemin 3, transcript variant B (Ns3), mRNA.

Price & Availability↑

CloneID	OFa00472
Clone ID Related Accession (Same CDS sequence)	NM_001297850.1 , NM_130559.3
Accession Version	NM_001297850.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1821bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	nucleostemin 3, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGCAAAA AGAACAAGGG CGGCGCCCCC AACCTGGGCC GCCAGTTGAT CAAGGATCGT 
TTCGGACACA CTCAGCGCCG CAAGGTGGAC AACGACACGA TGCTGCACAC CACCGAGCTG 
CAGGATGGCT ACGACTGGGG CCGCCTCAAC CTGTCCTCTG TCACTGAGGA GTCGTCGTTC 
CAGGCCTTCC TGCGCACGGC TGAGCTGGCC GGCACTGAGT TCCAGGCCGA GAAGCTGAAC 
ATCACGTTCG TGAACCCCAA GCAGCGCGTT GGACTGCTGA GCAAGACGCA GGAGCAGCGC 
ATGCACCAGA AGCACGACGA GCACCGTGAC CAGCTCAAGA TCCCGCGGCG GCCCAAGTGG 
ACCAAGGAGA CGAGCGCCGA GGAGCTAGTG CGAGCCGAGA ACGAGGCATT TCTCGACTGG 
CGCCGCGACC TGGCGCTGCT GCAGGAGGAC GAGGAGATCT TGATGACTCC GTATGAGAAG 
AACCTGGAGT TCTGGCGGCA GCTGTGGCGC GTAGTGGAGC GATCTGACGT CGTTGTGCAG 
ATCGTAGACG CCCGTAATCC GCTGCTGTTC CGCAGCGCCG ACCTTGAGCG CTACGTAAAG 
GAGGTGGAAC CTAGCAAGAT GAACATGATC CTGGTAAACA AGTCGGACCT GCTCACTGAG 
GAACAGCGTC GCCACTGGGC GGAGTACTTT GACAGCGAGG GTATTCGCAC GGCCTTCTAC 
TCGGCCACGC TGGTAGAGGA GGAGTTGAAG CGGGAGGCGG AGGAGTGCCT GGATTCCTTT 
CCCGAGGTGC AACAGCTGCG CAGAGCAGTT GAGGAGATAA AACAGTCGTT GGATAGCGTG 
GAGGACGCTT TGAACGTTAT CGAGCAGAAA TATAAGACGA TTCCCGAGAC TCAAAACGAT 
GAGCTGCCAC GATTGCCCGG GGACAAGAAC AGTCCCCGAC TGCTGTCCCG CCTGGAGCTC 
ATTGAGTTCC TGCGAAACAT CTACACCGGT CCCCGGCACA CAGAGCAGCA CGTGACCGTC 
GGAATGGTCG GCTACCCAAA CGTTGGCAAA AGTAGCACCA TCAACTCGCT GATGACAGTG 
AAGAAGGTGT CCGTTTCGGC CACGCCGGGC AAAACTAAAC GCTTCCAGAC GCTTTTCCTG 
GACAAGGACA TACTGCTCTG CGACTGTCCC GGTCTGGTGA TGCCGAGTTT TGTGTTAACC 
AAGGCGGACA TGTTGCTCAA CGGCATCCTG CCCATTGACC AAATGCGCGA TCACGTTCCC 
GCCGTCAACT TGCTGTGCGA GCGCATTCCG CGCCACGTCC TTGAAGACAA ATACGGTATT 
GTGATAGCCA AGCCGCTGGA AGGCGAGGAC ATGGAAAGGC CACCGCATTC GGAGGAGCTG 
CTGCTGGCTT ATGGATACAA TCGCGGTTTT ATGACCTCCA ACGGGCAGCC GGATCAGGCG 
CGTTCCGCCA GGTATGTGCT TAAAGACTAC GTCAATGGCA GACTGCTCTA CGCCATGAGC 
CCGCCCTCTG TACCGCAGAC CGAGTATCAC ACCTTTCCCG AACGCCAGCG CAGGGTCATC 
GAGGAATCAC AGCTGCCCGG CCAACAGCAG CGAGCCATGC GGATCAACAA AAGCACGTCC 
AAGGAGCTAG ACAACCAATT CTTCTCCGAC AAGCCTACGC ACGCCCACGT CAAAGGACGC 
ACTAACTTCC CTAACGTGCG CCTGGCCAAC GACGGGTCCC TAGTAGCCGG CAATGATCCG 
GCGGCCAAGC CCTGGCGTCA TGTGAAGAAG GAGAGGCGCG AGAAGCTGCG CAAAAAGTTC 
TCGCACCTGG ACGAGCACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001284779.1
CDS	106..1926
Translation	MGKKNKGGAPNLGRQLIKDRFGHTQRRKVDNDTMLHTTELQDGYDWGRLNLSSVTEESSFQAFLRTAELAGTEFQAEKLNITFVNPKQRVGLLSKTQEQRMHQKHDEHRDQLKIPRRPKWTKETSAEELVRAENEAFLDWRRDLALLQEDEEILMTPYEKNLEFWRQLWRVVERSDVVVQIVDARNPLLFRSADLERYVKEVEPSKMNMILVNKSDLLTEEQRRHWAEYFDSEGIRTAFYSATLVEEELKREAEECLDSFPEVQQLRRAVEEIKQSLDSVEDALNVIEQKYKTIPETQNDELPRLPGDKNSPRLLSRLELIEFLRNIYTGPRHTEQHVTVGMVGYPNVGKSSTINSLMTVKKVSVSATPGKTKRFQTLFLDKDILLCDCPGLVMPSFVLTKADMLLNGILPIDQMRDHVPAVNLLCERIPRHVLEDKYGIVIAKPLEGEDMERPPHSEELLLAYGYNRGFMTSNGQPDQARSARYVLKDYVNGRLLYAMSPPSVPQTEYHTFPERQRRVIEESQLPGQQQRAMRINKSTSKELDNQFFSDKPTHAHVKGRTNFPNVRLANDGSLVAGNDPAAKPWRHVKKERREKLRKKFSHLDEH

Target ORF information:

RefSeq Version	NM_001297850.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster nucleostemin 3, transcript variant B (Ns3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001297850.1

ORF Insert Sequence:

ATGGGCAAAA AGAACAAGGG CGGCGCCCCC AACCTGGGCC GCCAGTTGAT CAAGGATCGT 
TTCGGACACA CTCAGCGCCG CAAGGTGGAC AACGACACGA TGCTGCACAC CACCGAGCTG 
CAGGATGGCT ACGACTGGGG CCGCCTCAAC CTGTCCTCTG TCACTGAGGA GTCGTCGTTC 
CAGGCCTTCC TGCGCACGGC TGAGCTGGCC GGCACTGAGT TCCAGGCCGA GAAGCTGAAC 
ATCACGTTCG TGAACCCCAA GCAGCGCGTT GGACTGCTGA GCAAGACGCA GGAGCAGCGC 
ATGCACCAGA AGCACGACGA GCACCGTGAC CAGCTCAAGA TCCCGCGGCG GCCCAAGTGG 
ACCAAGGAGA CGAGCGCCGA GGAGCTAGTG CGAGCCGAGA ACGAGGCATT TCTCGACTGG 
CGCCGCGACC TGGCGCTGCT GCAGGAGGAC GAGGAGATCT TGATGACTCC GTATGAGAAG 
AACCTGGAGT TCTGGCGGCA GCTGTGGCGC GTAGTGGAGC GATCTGACGT CGTTGTGCAG 
ATCGTAGACG CCCGTAATCC GCTGCTGTTC CGCAGCGCCG ACCTTGAGCG CTACGTAAAG 
GAGGTGGAAC CTAGCAAGAT GAACATGATC CTGGTAAACA AGTCGGACCT GCTCACTGAG 
GAACAGCGTC GCCACTGGGC GGAGTACTTT GACAGCGAGG GTATTCGCAC GGCCTTCTAC 
TCGGCCACGC TGGTAGAGGA GGAGTTGAAG CGGGAGGCGG AGGAGTGCCT GGATTCCTTT 
CCCGAGGTGC AACAGCTGCG CAGAGCAGTT GAGGAGATAA AACAGTCGTT GGATAGCGTG 
GAGGACGCTT TGAACGTTAT CGAGCAGAAA TATAAGACGA TTCCCGAGAC TCAAAACGAT 
GAGCTGCCAC GATTGCCCGG GGACAAGAAC AGTCCCCGAC TGCTGTCCCG CCTGGAGCTC 
ATTGAGTTCC TGCGAAACAT CTACACCGGT CCCCGGCACA CAGAGCAGCA CGTGACCGTC 
GGAATGGTCG GCTACCCAAA CGTTGGCAAA AGTAGCACCA TCAACTCGCT GATGACAGTG 
AAGAAGGTGT CCGTTTCGGC CACGCCGGGC AAAACTAAAC GCTTCCAGAC GCTTTTCCTG 
GACAAGGACA TACTGCTCTG CGACTGTCCC GGTCTGGTGA TGCCGAGTTT TGTGTTAACC 
AAGGCGGACA TGTTGCTCAA CGGCATCCTG CCCATTGACC AAATGCGCGA TCACGTTCCC 
GCCGTCAACT TGCTGTGCGA GCGCATTCCG CGCCACGTCC TTGAAGACAA ATACGGTATT 
GTGATAGCCA AGCCGCTGGA AGGCGAGGAC ATGGAAAGGC CACCGCATTC GGAGGAGCTG 
CTGCTGGCTT ATGGATACAA TCGCGGTTTT ATGACCTCCA ACGGGCAGCC GGATCAGGCG 
CGTTCCGCCA GGTATGTGCT TAAAGACTAC GTCAATGGCA GACTGCTCTA CGCCATGAGC 
CCGCCCTCTG TACCGCAGAC CGAGTATCAC ACCTTTCCCG AACGCCAGCG CAGGGTCATC 
GAGGAATCAC AGCTGCCCGG CCAACAGCAG CGAGCCATGC GGATCAACAA AAGCACGTCC 
AAGGAGCTAG ACAACCAATT CTTCTCCGAC AAGCCTACGC ACGCCCACGT CAAAGGACGC 
ACTAACTTCC CTAACGTGCG CCTGGCCAAC GACGGGTCCC TAGTAGCCGG CAATGATCCG 
GCGGCCAAGC CCTGGCGTCA TGTGAAGAAG GAGAGGCGCG AGAAGCTGCG CAAAAAGTTC 
TCGCACCTGG ACGAGCACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ns3 ( NM_130559.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_569915.1 Drosophila melanogaster nucleostemin 3, transcript variant A (Ns3), mRNA.

Price & Availability↑

CloneID	OFa00472
Clone ID Related Accession (Same CDS sequence)	NM_001297850.1 , NM_130559.3
Accession Version	NM_130559.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1821bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	nucleostemin 3, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_130559.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGCAAAA AGAACAAGGG CGGCGCCCCC AACCTGGGCC GCCAGTTGAT CAAGGATCGT 
TTCGGACACA CTCAGCGCCG CAAGGTGGAC AACGACACGA TGCTGCACAC CACCGAGCTG 
CAGGATGGCT ACGACTGGGG CCGCCTCAAC CTGTCCTCTG TCACTGAGGA GTCGTCGTTC 
CAGGCCTTCC TGCGCACGGC TGAGCTGGCC GGCACTGAGT TCCAGGCCGA GAAGCTGAAC 
ATCACGTTCG TGAACCCCAA GCAGCGCGTT GGACTGCTGA GCAAGACGCA GGAGCAGCGC 
ATGCACCAGA AGCACGACGA GCACCGTGAC CAGCTCAAGA TCCCGCGGCG GCCCAAGTGG 
ACCAAGGAGA CGAGCGCCGA GGAGCTAGTG CGAGCCGAGA ACGAGGCATT TCTCGACTGG 
CGCCGCGACC TGGCGCTGCT GCAGGAGGAC GAGGAGATCT TGATGACTCC GTATGAGAAG 
AACCTGGAGT TCTGGCGGCA GCTGTGGCGC GTAGTGGAGC GATCTGACGT CGTTGTGCAG 
ATCGTAGACG CCCGTAATCC GCTGCTGTTC CGCAGCGCCG ACCTTGAGCG CTACGTAAAG 
GAGGTGGAAC CTAGCAAGAT GAACATGATC CTGGTAAACA AGTCGGACCT GCTCACTGAG 
GAACAGCGTC GCCACTGGGC GGAGTACTTT GACAGCGAGG GTATTCGCAC GGCCTTCTAC 
TCGGCCACGC TGGTAGAGGA GGAGTTGAAG CGGGAGGCGG AGGAGTGCCT GGATTCCTTT 
CCCGAGGTGC AACAGCTGCG CAGAGCAGTT GAGGAGATAA AACAGTCGTT GGATAGCGTG 
GAGGACGCTT TGAACGTTAT CGAGCAGAAA TATAAGACGA TTCCCGAGAC TCAAAACGAT 
GAGCTGCCAC GATTGCCCGG GGACAAGAAC AGTCCCCGAC TGCTGTCCCG CCTGGAGCTC 
ATTGAGTTCC TGCGAAACAT CTACACCGGT CCCCGGCACA CAGAGCAGCA CGTGACCGTC 
GGAATGGTCG GCTACCCAAA CGTTGGCAAA AGTAGCACCA TCAACTCGCT GATGACAGTG 
AAGAAGGTGT CCGTTTCGGC CACGCCGGGC AAAACTAAAC GCTTCCAGAC GCTTTTCCTG 
GACAAGGACA TACTGCTCTG CGACTGTCCC GGTCTGGTGA TGCCGAGTTT TGTGTTAACC 
AAGGCGGACA TGTTGCTCAA CGGCATCCTG CCCATTGACC AAATGCGCGA TCACGTTCCC 
GCCGTCAACT TGCTGTGCGA GCGCATTCCG CGCCACGTCC TTGAAGACAA ATACGGTATT 
GTGATAGCCA AGCCGCTGGA AGGCGAGGAC ATGGAAAGGC CACCGCATTC GGAGGAGCTG 
CTGCTGGCTT ATGGATACAA TCGCGGTTTT ATGACCTCCA ACGGGCAGCC GGATCAGGCG 
CGTTCCGCCA GGTATGTGCT TAAAGACTAC GTCAATGGCA GACTGCTCTA CGCCATGAGC 
CCGCCCTCTG TACCGCAGAC CGAGTATCAC ACCTTTCCCG AACGCCAGCG CAGGGTCATC 
GAGGAATCAC AGCTGCCCGG CCAACAGCAG CGAGCCATGC GGATCAACAA AAGCACGTCC 
AAGGAGCTAG ACAACCAATT CTTCTCCGAC AAGCCTACGC ACGCCCACGT CAAAGGACGC 
ACTAACTTCC CTAACGTGCG CCTGGCCAAC GACGGGTCCC TAGTAGCCGG CAATGATCCG 
GCGGCCAAGC CCTGGCGTCA TGTGAAGAAG GAGAGGCGCG AGAAGCTGCG CAAAAAGTTC 
TCGCACCTGG ACGAGCACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_569915.1
CDS	106..1926
Translation	MGKKNKGGAPNLGRQLIKDRFGHTQRRKVDNDTMLHTTELQDGYDWGRLNLSSVTEESSFQAFLRTAELAGTEFQAEKLNITFVNPKQRVGLLSKTQEQRMHQKHDEHRDQLKIPRRPKWTKETSAEELVRAENEAFLDWRRDLALLQEDEEILMTPYEKNLEFWRQLWRVVERSDVVVQIVDARNPLLFRSADLERYVKEVEPSKMNMILVNKSDLLTEEQRRHWAEYFDSEGIRTAFYSATLVEEELKREAEECLDSFPEVQQLRRAVEEIKQSLDSVEDALNVIEQKYKTIPETQNDELPRLPGDKNSPRLLSRLELIEFLRNIYTGPRHTEQHVTVGMVGYPNVGKSSTINSLMTVKKVSVSATPGKTKRFQTLFLDKDILLCDCPGLVMPSFVLTKADMLLNGILPIDQMRDHVPAVNLLCERIPRHVLEDKYGIVIAKPLEGEDMERPPHSEELLLAYGYNRGFMTSNGQPDQARSARYVLKDYVNGRLLYAMSPPSVPQTEYHTFPERQRRVIEESQLPGQQQRAMRINKSTSKELDNQFFSDKPTHAHVKGRTNFPNVRLANDGSLVAGNDPAAKPWRHVKKERREKLRKKFSHLDEH

Target ORF information:

RefSeq Version	NM_130559.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster nucleostemin 3, transcript variant A (Ns3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_130559.3

ORF Insert Sequence:

ATGGGCAAAA AGAACAAGGG CGGCGCCCCC AACCTGGGCC GCCAGTTGAT CAAGGATCGT 
TTCGGACACA CTCAGCGCCG CAAGGTGGAC AACGACACGA TGCTGCACAC CACCGAGCTG 
CAGGATGGCT ACGACTGGGG CCGCCTCAAC CTGTCCTCTG TCACTGAGGA GTCGTCGTTC 
CAGGCCTTCC TGCGCACGGC TGAGCTGGCC GGCACTGAGT TCCAGGCCGA GAAGCTGAAC 
ATCACGTTCG TGAACCCCAA GCAGCGCGTT GGACTGCTGA GCAAGACGCA GGAGCAGCGC 
ATGCACCAGA AGCACGACGA GCACCGTGAC CAGCTCAAGA TCCCGCGGCG GCCCAAGTGG 
ACCAAGGAGA CGAGCGCCGA GGAGCTAGTG CGAGCCGAGA ACGAGGCATT TCTCGACTGG 
CGCCGCGACC TGGCGCTGCT GCAGGAGGAC GAGGAGATCT TGATGACTCC GTATGAGAAG 
AACCTGGAGT TCTGGCGGCA GCTGTGGCGC GTAGTGGAGC GATCTGACGT CGTTGTGCAG 
ATCGTAGACG CCCGTAATCC GCTGCTGTTC CGCAGCGCCG ACCTTGAGCG CTACGTAAAG 
GAGGTGGAAC CTAGCAAGAT GAACATGATC CTGGTAAACA AGTCGGACCT GCTCACTGAG 
GAACAGCGTC GCCACTGGGC GGAGTACTTT GACAGCGAGG GTATTCGCAC GGCCTTCTAC 
TCGGCCACGC TGGTAGAGGA GGAGTTGAAG CGGGAGGCGG AGGAGTGCCT GGATTCCTTT 
CCCGAGGTGC AACAGCTGCG CAGAGCAGTT GAGGAGATAA AACAGTCGTT GGATAGCGTG 
GAGGACGCTT TGAACGTTAT CGAGCAGAAA TATAAGACGA TTCCCGAGAC TCAAAACGAT 
GAGCTGCCAC GATTGCCCGG GGACAAGAAC AGTCCCCGAC TGCTGTCCCG CCTGGAGCTC 
ATTGAGTTCC TGCGAAACAT CTACACCGGT CCCCGGCACA CAGAGCAGCA CGTGACCGTC 
GGAATGGTCG GCTACCCAAA CGTTGGCAAA AGTAGCACCA TCAACTCGCT GATGACAGTG 
AAGAAGGTGT CCGTTTCGGC CACGCCGGGC AAAACTAAAC GCTTCCAGAC GCTTTTCCTG 
GACAAGGACA TACTGCTCTG CGACTGTCCC GGTCTGGTGA TGCCGAGTTT TGTGTTAACC 
AAGGCGGACA TGTTGCTCAA CGGCATCCTG CCCATTGACC AAATGCGCGA TCACGTTCCC 
GCCGTCAACT TGCTGTGCGA GCGCATTCCG CGCCACGTCC TTGAAGACAA ATACGGTATT 
GTGATAGCCA AGCCGCTGGA AGGCGAGGAC ATGGAAAGGC CACCGCATTC GGAGGAGCTG 
CTGCTGGCTT ATGGATACAA TCGCGGTTTT ATGACCTCCA ACGGGCAGCC GGATCAGGCG 
CGTTCCGCCA GGTATGTGCT TAAAGACTAC GTCAATGGCA GACTGCTCTA CGCCATGAGC 
CCGCCCTCTG TACCGCAGAC CGAGTATCAC ACCTTTCCCG AACGCCAGCG CAGGGTCATC 
GAGGAATCAC AGCTGCCCGG CCAACAGCAG CGAGCCATGC GGATCAACAA AAGCACGTCC 
AAGGAGCTAG ACAACCAATT CTTCTCCGAC AAGCCTACGC ACGCCCACGT CAAAGGACGC 
ACTAACTTCC CTAACGTGCG CCTGGCCAAC GACGGGTCCC TAGTAGCCGG CAATGATCCG 
GCGGCCAAGC CCTGGCGTCA TGTGAAGAAG GAGAGGCGCG AGAAGCTGCG CAAAAAGTTC 
TCGCACCTGG ACGAGCACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Ns3 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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