CG16989 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	CG16989
Entrez Gene ID	31026
Full Name	CG16989 gene product from transcript CG16989-RB
Synonyms	Dmel\CG16989,EG:34F3.4
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 1C5-1C5

mRNA and Protein(s)

mRNA	Protein	Name
NM_001297832.1	NP_001284761.1	uncharacterized protein, isoform B
NM_130504.3	NP_569860.2	uncharacterized protein, isoform A

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	HEATR6	XP_001140673.1
Gallus gallus (chicken)	HEATR6	XP_415906.3
Drosophila melanogaster (fruit fly)	CG16989	NP_569860.2
Xenopus tropicalis (tropical clawed frog)	LOC100487263	XP_002939678.1
Homo sapiens (human)	HEATR6	NP_071353.4
Mus musculus (house mouse)	Heatr6	NP_663407.2
Danio rerio (zebrafish)	zgc:172359	NP_001107785.1
Canis lupus familiaris (dog)	HEATR6	XP_005624849.1
Rattus norvegicus (Norway rat)	Heatr6	NP_001073366.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007509	XP_308367.4
	HEATR6	XP_001115127.2
Bos taurus (cattle)	HEATR6	NP_001178374.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following CG16989 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CG16989 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa00404	NM_001297832.1 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant B (CG16989), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa00404	NM_130504.3 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant A (CG16989), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CG16989 ( NM_001297832.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001284761.1 Drosophila melanogaster uncharacterized protein, transcript variant B (CG16989), mRNA.

Price & Availability↑

CloneID	OFa00404
Clone ID Related Accession (Same CDS sequence)	NM_001297832.1 , NM_130504.3
Accession Version	NM_001297832.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2823bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTGACTG TGCTTAAATC CACCCCGAAG GAGAACCTGG CTGGGTTTTG GCCCCGATTG 
AGCGCATTAA CTCATGCCAA AGATATGGAC GTCGCTCTGT TGCTGCTGCA ATGCCAAGAT 
CGTGTGCTGG CCGAGCTGGA GGAATCGCCG GAGGTGGCCG AGCACAACCA GGGACTGGTT 
AACTCCCTCA TCAACATGCA CTTTGAGGGA CGCTGGCTGG TCGCCAAGGA GCAGTTGCCC 
CTGCTGCTGG CGCAGAGCCT GCAACTGATT CAGAAGCTGG TGACCAGGCA GCCCGACTAC 
GCCGCGCAAA GAGTCCCCGA ACTGATGGGG CTAGTCCAAT GCTATCTGCA GTATGGAGCT 
GAAGGAGAAA CCCACCTAAA GCCCCGGAAG TTGCCGCCCG CACAACAGTC GGCTGCCTAT 
GGGCAGGAGA AGGAAGAGGA AGACCAGCTG CTGGCCCAGA TGTCCCGCCC TTGTGGTCGC 
AAGAACAAAG TGCGCAAGAT GCGTTCGCTT GCCAAACAGC GCAACCAGGC GGTCAATGAA 
CACCATGAGC CGAATCCGCG AGAGCGTCTG GTACTGATGG GTAACCAGGA CTACGGCTGC 
TTAACTGGAG ACTCGGGCGA GAACGGGCTG CCCTCTGATT CGGATCAGCA GCAGACAGAA 
AACAAGCACC ATCGGCACCA AGAGGCCAAG GTGAGGATCT CCGCATTACA TTTGCTCAGC 
ACCCTGACCA ACCAACTGCC GCGCCGCTCG TTTTATGGCT ACTGGCACGT CCTGTTCCCT 
GACGGAGACG CGGGTGGTAC TCCCCACCTA CTGCTCCTTG GTCTGAAAGA CACAAATTTC 
CGTTGTCGAG CTCTGGCCCT GCAGGTGGGA GCGCAGCTGT TGTACGGTTC AAAGGCATTC 
CTCAGCCAGG CATGCTCGCT CGGACCCAGC AACTTTACGC CATTTGCCGT CAGCCTGGCC 
AGCTCTGTAT TGACCGCTTA TCGCATCTTG ATCGCTATCC TCGAGAGAGA GTATACACCG 
CCTGTGCTGA CCCAGTGCCT CAAGTGCTTA GCCGTTTTGG TGCAAGCCAC GCCCTTTCAT 
CAGCTGGAAA TGGGATTTGT CTACGAGTTT GTGGGCCACG TGAAAAAACT GATAAAGAGT 
AGTGACCCTC CGGTCGCCGT GTCCGCCTTA CTGGTCATGG AAATGCTGGT GGGCACATCC 
AAGCTTACAC CGGAGATTGC CAGCTCCATA GGACTGGCTC CAAGTCAGAG AAACCTTCAA 
ATGGAACACG CGAGTACGGC GGAAACATAC CAGGAGTTGT GCGACTCGGA TGCGGAGATG 
GAACTCGAGG AGGGGGATGA GCAGCAGCAG CCATCGCAGA ACAATGTGGT GAATCCCGTC 
CACACAGTGT CCCCAACCAT TCCGCGCAAT TCATGGCTGC TAAGGCAGGT CCTCCGCTGT 
CTGGAGGGCA TGAGAATTCC ATCCGCCGTG CGCTTGGAGT GTTTCCAGGT GCTTCAGGCC 
ATGGCCACAC ACATAGGCCT GCTGCGAGGA CATCAGGTGC GTTTGTCAAG GGTGATATCC 
GCTGGGCTGA CTGATCCCGC TACAGATGTA AAGCTGTACG CCGCACGCTG CTTGGATTCA 
GTTGGCTACC AGTTGGGCAG ACTGTTACCG GAACCGGCAG AACGGGAGCT GCAGATGTCG 
TTTTGGCTTA CCCTGCTGCC GGCCACCTAT GAAGCCTACG ATTCCGCCGG GTTCTCCTTG 
AAGTGCGCGC TCTGCGACGC CCTGTCCAAC ATGGGTGCTA TTAGCTTTGA GAGACTACCG 
GATGGCCAGA GGACCGCCCT GTTGGCCTTC CTAAGCGGTT GCGCCAGCGA CGATCATGAG 
GAAACACTGG TCAGGGTAGC CGCTTTGCGG GCTATGGCCG TCTACGTGCT GCATCCCTCG 
CTAAGGACGG ATCTTGGATT TGTGGAGAAT GCAGCGGAGC TGACATTGCG TATCTTAGGT 
GATTCCCAGC TCTTGGCTAG AATCAAGGCC GCATGGGCCC TGGGGAACAT CAGTGACGCA 
CTGGTGGCCG GTATTCCCAA CCAGACCGAA CGAATTTCCG CCGAACTTTT AGAACGCCTC 
ATCCAGGCAG CCACCAAGAG CTGTGCCGAT AACGACAAGG TCAGGGCCAA CGCGGTGCGT 
GCCCTGGGAA ATCTCTTGCA GATCTTGCAG GTCCAGCCCT TAGGAAACGG TGAACTGATG 
CAAGTGGCCA TGTCTAAGCT GCTAGACTGT GTAACATTGC CAGGCAACGC TAAGGTGAAG 
TGGAATGCAT GCTATGTCAT CGGAAACCTA GTGAAGCACA GGGCTTTCTT TGCCAACAAC 
CGCCTGGCTG GTATCTTATT TCCCACCCTG TGTCAGTTGG TGGTGCAGCA CGCTAACTTC 
AAGGTACGCA CCAATGCAGC CGGAGTTCTG CTGCAGGTCA AGCAGCGTGA GGACTTCAGT 
GCCCACTTTC CTCTGCTATG GCGTTCGCTC CTGGACGCTC TGGTGCGCTC TAATGTATTG 
GAAAGCTTTG AAGAGTACAA CCACCGGGAT GCACTTCAGC AACAGTTGTG CCTAGCCATT 
GCCCACCTAC TGTTGCTGGC CAGGAGCTCG GATCTGCCGA TGATGCGAGA GTCCCTAGAG 
GAGGATCGCC TAGAGGAGGT GCAGGCCACG TGGCGGCGCG TCGCATTCCG CATTGTTCCT 
GAGCAGTCGG ATCCGCTCTT CACCTGCAGT CCTCTGCTGC AGCAGCGTCT CCAAGGGGAT 
GTCAACAATC TGCAGCGCTG CGCCTTGGCG TTCATCACTA GCACACTCCG CCTGGACCCA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001284761.1
CDS	331..3153
Translation	MLTVLKSTPKENLAGFWPRLSALTHAKDMDVALLLLQCQDRVLAELEESPEVAEHNQGLVNSLINMHFEGRWLVAKEQLPLLLAQSLQLIQKLVTRQPDYAAQRVPELMGLVQCYLQYGAEGETHLKPRKLPPAQQSAAYGQEKEEEDQLLAQMSRPCGRKNKVRKMRSLAKQRNQAVNEHHEPNPRERLVLMGNQDYGCLTGDSGENGLPSDSDQQQTENKHHRHQEAKVRISALHLLSTLTNQLPRRSFYGYWHVLFPDGDAGGTPHLLLLGLKDTNFRCRALALQVGAQLLYGSKAFLSQACSLGPSNFTPFAVSLASSVLTAYRILIAILEREYTPPVLTQCLKCLAVLVQATPFHQLEMGFVYEFVGHVKKLIKSSDPPVAVSALLVMEMLVGTSKLTPEIASSIGLAPSQRNLQMEHASTAETYQELCDSDAEMELEEGDEQQQPSQNNVVNPVHTVSPTIPRNSWLLRQVLRCLEGMRIPSAVRLECFQVLQAMATHIGLLRGHQVRLSRVISAGLTDPATDVKLYAARCLDSVGYQLGRLLPEPAERELQMSFWLTLLPATYEAYDSAGFSLKCALCDALSNMGAISFERLPDGQRTALLAFLSGCASDDHEETLVRVAALRAMAVYVLHPSLRTDLGFVENAAELTLRILGDSQLLARIKAAWALGNISDALVAGIPNQTERISAELLERLIQAATKSCADNDKVRANAVRALGNLLQILQVQPLGNGELMQVAMSKLLDCVTLPGNAKVKWNACYVIGNLVKHRAFFANNRLAGILFPTLCQLVVQHANFKVRTNAAGVLLQVKQREDFSAHFPLLWRSLLDALVRSNVLESFEEYNHRDALQQQLCLAIAHLLLLARSSDLPMMRESLEEDRLEEVQATWRRVAFRIVPEQSDPLFTCSPLLQQRLQGDVNNLQRCALAFITSTLRLDP

Target ORF information:

RefSeq Version	NM_001297832.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant B (CG16989), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001297832.1

ORF Insert Sequence:

ATGTTGACTG TGCTTAAATC CACCCCGAAG GAGAACCTGG CTGGGTTTTG GCCCCGATTG 
AGCGCATTAA CTCATGCCAA AGATATGGAC GTCGCTCTGT TGCTGCTGCA ATGCCAAGAT 
CGTGTGCTGG CCGAGCTGGA GGAATCGCCG GAGGTGGCCG AGCACAACCA GGGACTGGTT 
AACTCCCTCA TCAACATGCA CTTTGAGGGA CGCTGGCTGG TCGCCAAGGA GCAGTTGCCC 
CTGCTGCTGG CGCAGAGCCT GCAACTGATT CAGAAGCTGG TGACCAGGCA GCCCGACTAC 
GCCGCGCAAA GAGTCCCCGA ACTGATGGGG CTAGTCCAAT GCTATCTGCA GTATGGAGCT 
GAAGGAGAAA CCCACCTAAA GCCCCGGAAG TTGCCGCCCG CACAACAGTC GGCTGCCTAT 
GGGCAGGAGA AGGAAGAGGA AGACCAGCTG CTGGCCCAGA TGTCCCGCCC TTGTGGTCGC 
AAGAACAAAG TGCGCAAGAT GCGTTCGCTT GCCAAACAGC GCAACCAGGC GGTCAATGAA 
CACCATGAGC CGAATCCGCG AGAGCGTCTG GTACTGATGG GTAACCAGGA CTACGGCTGC 
TTAACTGGAG ACTCGGGCGA GAACGGGCTG CCCTCTGATT CGGATCAGCA GCAGACAGAA 
AACAAGCACC ATCGGCACCA AGAGGCCAAG GTGAGGATCT CCGCATTACA TTTGCTCAGC 
ACCCTGACCA ACCAACTGCC GCGCCGCTCG TTTTATGGCT ACTGGCACGT CCTGTTCCCT 
GACGGAGACG CGGGTGGTAC TCCCCACCTA CTGCTCCTTG GTCTGAAAGA CACAAATTTC 
CGTTGTCGAG CTCTGGCCCT GCAGGTGGGA GCGCAGCTGT TGTACGGTTC AAAGGCATTC 
CTCAGCCAGG CATGCTCGCT CGGACCCAGC AACTTTACGC CATTTGCCGT CAGCCTGGCC 
AGCTCTGTAT TGACCGCTTA TCGCATCTTG ATCGCTATCC TCGAGAGAGA GTATACACCG 
CCTGTGCTGA CCCAGTGCCT CAAGTGCTTA GCCGTTTTGG TGCAAGCCAC GCCCTTTCAT 
CAGCTGGAAA TGGGATTTGT CTACGAGTTT GTGGGCCACG TGAAAAAACT GATAAAGAGT 
AGTGACCCTC CGGTCGCCGT GTCCGCCTTA CTGGTCATGG AAATGCTGGT GGGCACATCC 
AAGCTTACAC CGGAGATTGC CAGCTCCATA GGACTGGCTC CAAGTCAGAG AAACCTTCAA 
ATGGAACACG CGAGTACGGC GGAAACATAC CAGGAGTTGT GCGACTCGGA TGCGGAGATG 
GAACTCGAGG AGGGGGATGA GCAGCAGCAG CCATCGCAGA ACAATGTGGT GAATCCCGTC 
CACACAGTGT CCCCAACCAT TCCGCGCAAT TCATGGCTGC TAAGGCAGGT CCTCCGCTGT 
CTGGAGGGCA TGAGAATTCC ATCCGCCGTG CGCTTGGAGT GTTTCCAGGT GCTTCAGGCC 
ATGGCCACAC ACATAGGCCT GCTGCGAGGA CATCAGGTGC GTTTGTCAAG GGTGATATCC 
GCTGGGCTGA CTGATCCCGC TACAGATGTA AAGCTGTACG CCGCACGCTG CTTGGATTCA 
GTTGGCTACC AGTTGGGCAG ACTGTTACCG GAACCGGCAG AACGGGAGCT GCAGATGTCG 
TTTTGGCTTA CCCTGCTGCC GGCCACCTAT GAAGCCTACG ATTCCGCCGG GTTCTCCTTG 
AAGTGCGCGC TCTGCGACGC CCTGTCCAAC ATGGGTGCTA TTAGCTTTGA GAGACTACCG 
GATGGCCAGA GGACCGCCCT GTTGGCCTTC CTAAGCGGTT GCGCCAGCGA CGATCATGAG 
GAAACACTGG TCAGGGTAGC CGCTTTGCGG GCTATGGCCG TCTACGTGCT GCATCCCTCG 
CTAAGGACGG ATCTTGGATT TGTGGAGAAT GCAGCGGAGC TGACATTGCG TATCTTAGGT 
GATTCCCAGC TCTTGGCTAG AATCAAGGCC GCATGGGCCC TGGGGAACAT CAGTGACGCA 
CTGGTGGCCG GTATTCCCAA CCAGACCGAA CGAATTTCCG CCGAACTTTT AGAACGCCTC 
ATCCAGGCAG CCACCAAGAG CTGTGCCGAT AACGACAAGG TCAGGGCCAA CGCGGTGCGT 
GCCCTGGGAA ATCTCTTGCA GATCTTGCAG GTCCAGCCCT TAGGAAACGG TGAACTGATG 
CAAGTGGCCA TGTCTAAGCT GCTAGACTGT GTAACATTGC CAGGCAACGC TAAGGTGAAG 
TGGAATGCAT GCTATGTCAT CGGAAACCTA GTGAAGCACA GGGCTTTCTT TGCCAACAAC 
CGCCTGGCTG GTATCTTATT TCCCACCCTG TGTCAGTTGG TGGTGCAGCA CGCTAACTTC 
AAGGTACGCA CCAATGCAGC CGGAGTTCTG CTGCAGGTCA AGCAGCGTGA GGACTTCAGT 
GCCCACTTTC CTCTGCTATG GCGTTCGCTC CTGGACGCTC TGGTGCGCTC TAATGTATTG 
GAAAGCTTTG AAGAGTACAA CCACCGGGAT GCACTTCAGC AACAGTTGTG CCTAGCCATT 
GCCCACCTAC TGTTGCTGGC CAGGAGCTCG GATCTGCCGA TGATGCGAGA GTCCCTAGAG 
GAGGATCGCC TAGAGGAGGT GCAGGCCACG TGGCGGCGCG TCGCATTCCG CATTGTTCCT 
GAGCAGTCGG ATCCGCTCTT CACCTGCAGT CCTCTGCTGC AGCAGCGTCT CCAAGGGGAT 
GTCAACAATC TGCAGCGCTG CGCCTTGGCG TTCATCACTA GCACACTCCG CCTGGACCCA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CG16989 ( NM_130504.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_569860.2 Drosophila melanogaster uncharacterized protein, transcript variant A (CG16989), mRNA.

Price & Availability↑

CloneID	OFa00404
Clone ID Related Accession (Same CDS sequence)	NM_001297832.1 , NM_130504.3
Accession Version	NM_130504.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2823bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_130504.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTGACTG TGCTTAAATC CACCCCGAAG GAGAACCTGG CTGGGTTTTG GCCCCGATTG 
AGCGCATTAA CTCATGCCAA AGATATGGAC GTCGCTCTGT TGCTGCTGCA ATGCCAAGAT 
CGTGTGCTGG CCGAGCTGGA GGAATCGCCG GAGGTGGCCG AGCACAACCA GGGACTGGTT 
AACTCCCTCA TCAACATGCA CTTTGAGGGA CGCTGGCTGG TCGCCAAGGA GCAGTTGCCC 
CTGCTGCTGG CGCAGAGCCT GCAACTGATT CAGAAGCTGG TGACCAGGCA GCCCGACTAC 
GCCGCGCAAA GAGTCCCCGA ACTGATGGGG CTAGTCCAAT GCTATCTGCA GTATGGAGCT 
GAAGGAGAAA CCCACCTAAA GCCCCGGAAG TTGCCGCCCG CACAACAGTC GGCTGCCTAT 
GGGCAGGAGA AGGAAGAGGA AGACCAGCTG CTGGCCCAGA TGTCCCGCCC TTGTGGTCGC 
AAGAACAAAG TGCGCAAGAT GCGTTCGCTT GCCAAACAGC GCAACCAGGC GGTCAATGAA 
CACCATGAGC CGAATCCGCG AGAGCGTCTG GTACTGATGG GTAACCAGGA CTACGGCTGC 
TTAACTGGAG ACTCGGGCGA GAACGGGCTG CCCTCTGATT CGGATCAGCA GCAGACAGAA 
AACAAGCACC ATCGGCACCA AGAGGCCAAG GTGAGGATCT CCGCATTACA TTTGCTCAGC 
ACCCTGACCA ACCAACTGCC GCGCCGCTCG TTTTATGGCT ACTGGCACGT CCTGTTCCCT 
GACGGAGACG CGGGTGGTAC TCCCCACCTA CTGCTCCTTG GTCTGAAAGA CACAAATTTC 
CGTTGTCGAG CTCTGGCCCT GCAGGTGGGA GCGCAGCTGT TGTACGGTTC AAAGGCATTC 
CTCAGCCAGG CATGCTCGCT CGGACCCAGC AACTTTACGC CATTTGCCGT CAGCCTGGCC 
AGCTCTGTAT TGACCGCTTA TCGCATCTTG ATCGCTATCC TCGAGAGAGA GTATACACCG 
CCTGTGCTGA CCCAGTGCCT CAAGTGCTTA GCCGTTTTGG TGCAAGCCAC GCCCTTTCAT 
CAGCTGGAAA TGGGATTTGT CTACGAGTTT GTGGGCCACG TGAAAAAACT GATAAAGAGT 
AGTGACCCTC CGGTCGCCGT GTCCGCCTTA CTGGTCATGG AAATGCTGGT GGGCACATCC 
AAGCTTACAC CGGAGATTGC CAGCTCCATA GGACTGGCTC CAAGTCAGAG AAACCTTCAA 
ATGGAACACG CGAGTACGGC GGAAACATAC CAGGAGTTGT GCGACTCGGA TGCGGAGATG 
GAACTCGAGG AGGGGGATGA GCAGCAGCAG CCATCGCAGA ACAATGTGGT GAATCCCGTC 
CACACAGTGT CCCCAACCAT TCCGCGCAAT TCATGGCTGC TAAGGCAGGT CCTCCGCTGT 
CTGGAGGGCA TGAGAATTCC ATCCGCCGTG CGCTTGGAGT GTTTCCAGGT GCTTCAGGCC 
ATGGCCACAC ACATAGGCCT GCTGCGAGGA CATCAGGTGC GTTTGTCAAG GGTGATATCC 
GCTGGGCTGA CTGATCCCGC TACAGATGTA AAGCTGTACG CCGCACGCTG CTTGGATTCA 
GTTGGCTACC AGTTGGGCAG ACTGTTACCG GAACCGGCAG AACGGGAGCT GCAGATGTCG 
TTTTGGCTTA CCCTGCTGCC GGCCACCTAT GAAGCCTACG ATTCCGCCGG GTTCTCCTTG 
AAGTGCGCGC TCTGCGACGC CCTGTCCAAC ATGGGTGCTA TTAGCTTTGA GAGACTACCG 
GATGGCCAGA GGACCGCCCT GTTGGCCTTC CTAAGCGGTT GCGCCAGCGA CGATCATGAG 
GAAACACTGG TCAGGGTAGC CGCTTTGCGG GCTATGGCCG TCTACGTGCT GCATCCCTCG 
CTAAGGACGG ATCTTGGATT TGTGGAGAAT GCAGCGGAGC TGACATTGCG TATCTTAGGT 
GATTCCCAGC TCTTGGCTAG AATCAAGGCC GCATGGGCCC TGGGGAACAT CAGTGACGCA 
CTGGTGGCCG GTATTCCCAA CCAGACCGAA CGAATTTCCG CCGAACTTTT AGAACGCCTC 
ATCCAGGCAG CCACCAAGAG CTGTGCCGAT AACGACAAGG TCAGGGCCAA CGCGGTGCGT 
GCCCTGGGAA ATCTCTTGCA GATCTTGCAG GTCCAGCCCT TAGGAAACGG TGAACTGATG 
CAAGTGGCCA TGTCTAAGCT GCTAGACTGT GTAACATTGC CAGGCAACGC TAAGGTGAAG 
TGGAATGCAT GCTATGTCAT CGGAAACCTA GTGAAGCACA GGGCTTTCTT TGCCAACAAC 
CGCCTGGCTG GTATCTTATT TCCCACCCTG TGTCAGTTGG TGGTGCAGCA CGCTAACTTC 
AAGGTACGCA CCAATGCAGC CGGAGTTCTG CTGCAGGTCA AGCAGCGTGA GGACTTCAGT 
GCCCACTTTC CTCTGCTATG GCGTTCGCTC CTGGACGCTC TGGTGCGCTC TAATGTATTG 
GAAAGCTTTG AAGAGTACAA CCACCGGGAT GCACTTCAGC AACAGTTGTG CCTAGCCATT 
GCCCACCTAC TGTTGCTGGC CAGGAGCTCG GATCTGCCGA TGATGCGAGA GTCCCTAGAG 
GAGGATCGCC TAGAGGAGGT GCAGGCCACG TGGCGGCGCG TCGCATTCCG CATTGTTCCT 
GAGCAGTCGG ATCCGCTCTT CACCTGCAGT CCTCTGCTGC AGCAGCGTCT CCAAGGGGAT 
GTCAACAATC TGCAGCGCTG CGCCTTGGCG TTCATCACTA GCACACTCCG CCTGGACCCA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_569860.2
CDS	331..3153
Translation	MLTVLKSTPKENLAGFWPRLSALTHAKDMDVALLLLQCQDRVLAELEESPEVAEHNQGLVNSLINMHFEGRWLVAKEQLPLLLAQSLQLIQKLVTRQPDYAAQRVPELMGLVQCYLQYGAEGETHLKPRKLPPAQQSAAYGQEKEEEDQLLAQMSRPCGRKNKVRKMRSLAKQRNQAVNEHHEPNPRERLVLMGNQDYGCLTGDSGENGLPSDSDQQQTENKHHRHQEAKVRISALHLLSTLTNQLPRRSFYGYWHVLFPDGDAGGTPHLLLLGLKDTNFRCRALALQVGAQLLYGSKAFLSQACSLGPSNFTPFAVSLASSVLTAYRILIAILEREYTPPVLTQCLKCLAVLVQATPFHQLEMGFVYEFVGHVKKLIKSSDPPVAVSALLVMEMLVGTSKLTPEIASSIGLAPSQRNLQMEHASTAETYQELCDSDAEMELEEGDEQQQPSQNNVVNPVHTVSPTIPRNSWLLRQVLRCLEGMRIPSAVRLECFQVLQAMATHIGLLRGHQVRLSRVISAGLTDPATDVKLYAARCLDSVGYQLGRLLPEPAERELQMSFWLTLLPATYEAYDSAGFSLKCALCDALSNMGAISFERLPDGQRTALLAFLSGCASDDHEETLVRVAALRAMAVYVLHPSLRTDLGFVENAAELTLRILGDSQLLARIKAAWALGNISDALVAGIPNQTERISAELLERLIQAATKSCADNDKVRANAVRALGNLLQILQVQPLGNGELMQVAMSKLLDCVTLPGNAKVKWNACYVIGNLVKHRAFFANNRLAGILFPTLCQLVVQHANFKVRTNAAGVLLQVKQREDFSAHFPLLWRSLLDALVRSNVLESFEEYNHRDALQQQLCLAIAHLLLLARSSDLPMMRESLEEDRLEEVQATWRRVAFRIVPEQSDPLFTCSPLLQQRLQGDVNNLQRCALAFITSTLRLDP

Target ORF information:

RefSeq Version	NM_130504.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant A (CG16989), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_130504.3

ORF Insert Sequence:

ATGTTGACTG TGCTTAAATC CACCCCGAAG GAGAACCTGG CTGGGTTTTG GCCCCGATTG 
AGCGCATTAA CTCATGCCAA AGATATGGAC GTCGCTCTGT TGCTGCTGCA ATGCCAAGAT 
CGTGTGCTGG CCGAGCTGGA GGAATCGCCG GAGGTGGCCG AGCACAACCA GGGACTGGTT 
AACTCCCTCA TCAACATGCA CTTTGAGGGA CGCTGGCTGG TCGCCAAGGA GCAGTTGCCC 
CTGCTGCTGG CGCAGAGCCT GCAACTGATT CAGAAGCTGG TGACCAGGCA GCCCGACTAC 
GCCGCGCAAA GAGTCCCCGA ACTGATGGGG CTAGTCCAAT GCTATCTGCA GTATGGAGCT 
GAAGGAGAAA CCCACCTAAA GCCCCGGAAG TTGCCGCCCG CACAACAGTC GGCTGCCTAT 
GGGCAGGAGA AGGAAGAGGA AGACCAGCTG CTGGCCCAGA TGTCCCGCCC TTGTGGTCGC 
AAGAACAAAG TGCGCAAGAT GCGTTCGCTT GCCAAACAGC GCAACCAGGC GGTCAATGAA 
CACCATGAGC CGAATCCGCG AGAGCGTCTG GTACTGATGG GTAACCAGGA CTACGGCTGC 
TTAACTGGAG ACTCGGGCGA GAACGGGCTG CCCTCTGATT CGGATCAGCA GCAGACAGAA 
AACAAGCACC ATCGGCACCA AGAGGCCAAG GTGAGGATCT CCGCATTACA TTTGCTCAGC 
ACCCTGACCA ACCAACTGCC GCGCCGCTCG TTTTATGGCT ACTGGCACGT CCTGTTCCCT 
GACGGAGACG CGGGTGGTAC TCCCCACCTA CTGCTCCTTG GTCTGAAAGA CACAAATTTC 
CGTTGTCGAG CTCTGGCCCT GCAGGTGGGA GCGCAGCTGT TGTACGGTTC AAAGGCATTC 
CTCAGCCAGG CATGCTCGCT CGGACCCAGC AACTTTACGC CATTTGCCGT CAGCCTGGCC 
AGCTCTGTAT TGACCGCTTA TCGCATCTTG ATCGCTATCC TCGAGAGAGA GTATACACCG 
CCTGTGCTGA CCCAGTGCCT CAAGTGCTTA GCCGTTTTGG TGCAAGCCAC GCCCTTTCAT 
CAGCTGGAAA TGGGATTTGT CTACGAGTTT GTGGGCCACG TGAAAAAACT GATAAAGAGT 
AGTGACCCTC CGGTCGCCGT GTCCGCCTTA CTGGTCATGG AAATGCTGGT GGGCACATCC 
AAGCTTACAC CGGAGATTGC CAGCTCCATA GGACTGGCTC CAAGTCAGAG AAACCTTCAA 
ATGGAACACG CGAGTACGGC GGAAACATAC CAGGAGTTGT GCGACTCGGA TGCGGAGATG 
GAACTCGAGG AGGGGGATGA GCAGCAGCAG CCATCGCAGA ACAATGTGGT GAATCCCGTC 
CACACAGTGT CCCCAACCAT TCCGCGCAAT TCATGGCTGC TAAGGCAGGT CCTCCGCTGT 
CTGGAGGGCA TGAGAATTCC ATCCGCCGTG CGCTTGGAGT GTTTCCAGGT GCTTCAGGCC 
ATGGCCACAC ACATAGGCCT GCTGCGAGGA CATCAGGTGC GTTTGTCAAG GGTGATATCC 
GCTGGGCTGA CTGATCCCGC TACAGATGTA AAGCTGTACG CCGCACGCTG CTTGGATTCA 
GTTGGCTACC AGTTGGGCAG ACTGTTACCG GAACCGGCAG AACGGGAGCT GCAGATGTCG 
TTTTGGCTTA CCCTGCTGCC GGCCACCTAT GAAGCCTACG ATTCCGCCGG GTTCTCCTTG 
AAGTGCGCGC TCTGCGACGC CCTGTCCAAC ATGGGTGCTA TTAGCTTTGA GAGACTACCG 
GATGGCCAGA GGACCGCCCT GTTGGCCTTC CTAAGCGGTT GCGCCAGCGA CGATCATGAG 
GAAACACTGG TCAGGGTAGC CGCTTTGCGG GCTATGGCCG TCTACGTGCT GCATCCCTCG 
CTAAGGACGG ATCTTGGATT TGTGGAGAAT GCAGCGGAGC TGACATTGCG TATCTTAGGT 
GATTCCCAGC TCTTGGCTAG AATCAAGGCC GCATGGGCCC TGGGGAACAT CAGTGACGCA 
CTGGTGGCCG GTATTCCCAA CCAGACCGAA CGAATTTCCG CCGAACTTTT AGAACGCCTC 
ATCCAGGCAG CCACCAAGAG CTGTGCCGAT AACGACAAGG TCAGGGCCAA CGCGGTGCGT 
GCCCTGGGAA ATCTCTTGCA GATCTTGCAG GTCCAGCCCT TAGGAAACGG TGAACTGATG 
CAAGTGGCCA TGTCTAAGCT GCTAGACTGT GTAACATTGC CAGGCAACGC TAAGGTGAAG 
TGGAATGCAT GCTATGTCAT CGGAAACCTA GTGAAGCACA GGGCTTTCTT TGCCAACAAC 
CGCCTGGCTG GTATCTTATT TCCCACCCTG TGTCAGTTGG TGGTGCAGCA CGCTAACTTC 
AAGGTACGCA CCAATGCAGC CGGAGTTCTG CTGCAGGTCA AGCAGCGTGA GGACTTCAGT 
GCCCACTTTC CTCTGCTATG GCGTTCGCTC CTGGACGCTC TGGTGCGCTC TAATGTATTG 
GAAAGCTTTG AAGAGTACAA CCACCGGGAT GCACTTCAGC AACAGTTGTG CCTAGCCATT 
GCCCACCTAC TGTTGCTGGC CAGGAGCTCG GATCTGCCGA TGATGCGAGA GTCCCTAGAG 
GAGGATCGCC TAGAGGAGGT GCAGGCCACG TGGCGGCGCG TCGCATTCCG CATTGTTCCT 
GAGCAGTCGG ATCCGCTCTT CACCTGCAGT CCTCTGCTGC AGCAGCGTCT CCAAGGGGAT 
GTCAACAATC TGCAGCGCTG CGCCTTGGCG TTCATCACTA GCACACTCCG CCTGGACCCA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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CG16989 cDNA ORF clone, Drosophila melanogaster(fruit fly)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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