Roc1a cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Roc1a
Entrez Gene ID	31014
Full Name	Regulator of cullins 1a
Synonyms	BEST:CK01110,CG16982,CK01110,Dmel\CG16982,EG:115C2.11,ROC1,Rbx1,Rbx1/Roc,Roc,Roc1alpha,anon-EST:Posey65,dRbx1,dRoc1,dRoc1a
General protein information	Preferred Names Regulator of cullins 1a Names CG16982 gene product from transcript CG16982-RC CG16982-PA CG16982-PC CG16982-PD Roc1a-PA Roc1a-PC Roc1a-PD regulator of cullins 1a
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 1B13-1B13

mRNA and Protein(s)

mRNA	Protein	Name
NM_001144671.3	NP_001138143.2	regulator of cullins 1a, isoform C
NM_001297825.1	NP_001284754.1	regulator of cullins 1a, isoform D
NM_130496.4	NP_569852.1	regulator of cullins 1a, isoform A

Pathways from BioSystems

Homologies

Homo sapiens (human)	RBX1	NP_055063.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005202	XP_001688524.1
Arabidopsis thaliana (thale cress)	RBX1	NP_001154725.1
Xenopus tropicalis (tropical clawed frog)	rbx1	XP_002934772.2
Pan troglodytes (chimpanzee)	RBX1	XP_515153.2
Canis lupus familiaris (dog)	RBX1	XP_531722.2
Mus musculus (house mouse)	Gm9840	XP_006525295.1
Danio rerio (zebrafish)	rbx1	NP_001077013.1
Bos taurus (cattle)	RBX1	NP_001039706.1
Drosophila melanogaster (fruit fly)	Roc1a	NP_569852.1
Magnaporthe oryzae (rice blast fungus)	MGG_08844	XP_003713864.1
Gallus gallus (chicken)	RBX1	NP_001186179.1
Schizosaccharomyces pombe (fission yeast)	rbx1	NP_593388.1
	LOC710252	XP_001098854.2
Caenorhabditis elegans (roundworm)	rbx-1	NP_505496.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

BRWD3 functions as a Damage-specific DNA binding protein 1 (DDB1)- and CULLIN (CUL)4-associated factor in a Cullin4-RING Finger E3 Ligase (CRL4) that mediates light-dependent binding of dCRY to CUL4-ROC1-DDB1-BRWD3, inducing ubiquitylation of dCRY
Title: Ramshackle (Brwd3) promotes light-induced ubiquitylation of Drosophila Cryptochrome by DDB1-CUL4-ROC1 E3 ligase complex.

Roc1b, Roc1a and Roc2 proteins play nonredundant roles during development
Title: Targeted disruption of Drosophila Roc1b reveals functional differences in the Roc subunit of Cullin-dependent E3 ubiquitin ligases.

Roc1a encodes a RING-H2 protein with a unique function in processing the Hh signal transducer Ci by the SCF E3 ubiquitin ligase.
Title: Drosophila Roc1a encodes a RING-H2 protein with a unique function in processing the Hh signal transducer Ci by the SCF E3 ubiquitin ligase.

The following Roc1a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Roc1a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa00391	NM_001144671.3 Latest version!	Drosophila melanogaster regulator of cullins 1a, transcript variant C (Roc1a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OFa00392	NM_001297825.1 Latest version!	Drosophila melanogaster regulator of cullins 1a, transcript variant D (Roc1a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OFa00392	NM_130496.4 Latest version!	Drosophila melanogaster regulator of cullins 1a, transcript variant A (Roc1a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Roc1a ( NM_001144671.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001138143.2 Drosophila melanogaster regulator of cullins 1a, transcript variant C (Roc1a), mRNA.

Price & Availability↑

CloneID	OFa00391
Clone ID Related Accession (Same CDS sequence)	NM_001144671.3
Accession Version	NM_001144671.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 411bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	regulator of cullins 1a, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_001144671.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAAGTCG ACGAGGATGG ATACGAGGTT CCCTCCAGCA GCAGCAAGGG CGATAAGAAG 
CGCTTTGAGG TGAAGAAGGT AAGTGGTCAG CAAAAAAGCA GGGTCATTGT CAATGAATGT 
ACTGATGGTA ACACTTCATC CTTTCCGCTT CGCCGAAAAC AGTGGAACGC CGTGGCTCTG 
TGGGCCTGGG ACATCGTGGT GGACAACTGC GCCATCTGCC GCAACCACAT CATGGACTTG 
TGCATCGAGT GTCAGGCGAA CCAGGCGTCC GCCACTAGCG AGGAGTGCAC CGTGGCCTGG 
GGCGTCTGCA ACCACGCCTT CCATTTCCAC TGCATCTCTC GCTGGCTAAA GACGCGCCAG 
GTATGCCCAC TGGACAACCG CGAGTGGGAT TTCCAGAAGT ACGGCCACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001138143.2
CDS	145..555
Translation	MEVDEDGYEVPSSSSKGDKKRFEVKKVSGQQKSRVIVNECTDGNTSSFPLRRKQWNAVALWAWDIVVDNCAICRNHIMDLCIECQANQASATSEECTVAWGVCNHAFHFHCISRWLKTRQVCPLDNREWDFQKYGH

Target ORF information:

RefSeq Version	NM_001144671.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster regulator of cullins 1a, transcript variant C (Roc1a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001144671.3

ORF Insert Sequence:

ATGGAAGTCG ACGAGGATGG ATACGAGGTT CCCTCCAGCA GCAGCAAGGG CGATAAGAAG 
CGCTTTGAGG TGAAGAAGGT AAGTGGTCAG CAAAAAAGCA GGGTCATTGT CAATGAATGT 
ACTGATGGTA ACACTTCATC CTTTCCGCTT CGCCGAAAAC AGTGGAACGC CGTGGCTCTG 
TGGGCCTGGG ACATCGTGGT GGACAACTGC GCCATCTGCC GCAACCACAT CATGGACTTG 
TGCATCGAGT GTCAGGCGAA CCAGGCGTCC GCCACTAGCG AGGAGTGCAC CGTGGCCTGG 
GGCGTCTGCA ACCACGCCTT CCATTTCCAC TGCATCTCTC GCTGGCTAAA GACGCGCCAG 
GTATGCCCAC TGGACAACCG CGAGTGGGAT TTCCAGAAGT ACGGCCACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Roc1a ( NM_001297825.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001284754.1 Drosophila melanogaster regulator of cullins 1a, transcript variant D (Roc1a), mRNA.

Price & Availability↑

CloneID	OFa00392
Clone ID Related Accession (Same CDS sequence)	NM_001297825.1 , NM_130496.4
Accession Version	NM_001297825.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 327bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	regulator of cullins 1a, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAAGTCG ACGAGGATGG ATACGAGGTT CCCTCCAGCA GCAGCAAGGG CGATAAGAAG 
CGCTTTGAGG TGAAGAAGTG GAACGCCGTG GCTCTGTGGG CCTGGGACAT CGTGGTGGAC 
AACTGCGCCA TCTGCCGCAA CCACATCATG GACTTGTGCA TCGAGTGTCA GGCGAACCAG 
GCGTCCGCCA CTAGCGAGGA GTGCACCGTG GCCTGGGGCG TCTGCAACCA CGCCTTCCAT 
TTCCACTGCA TCTCTCGCTG GCTAAAGACG CGCCAGGTAT GCCCACTGGA CAACCGCGAG 
TGGGATTTCC AGAAGTACGG CCACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001284754.1
CDS	145..471
Translation	MEVDEDGYEVPSSSSKGDKKRFEVKKWNAVALWAWDIVVDNCAICRNHIMDLCIECQANQASATSEECTVAWGVCNHAFHFHCISRWLKTRQVCPLDNREWDFQKYGH

Target ORF information:

RefSeq Version	NM_001297825.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster regulator of cullins 1a, transcript variant D (Roc1a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001297825.1

ORF Insert Sequence:

ATGGAAGTCG ACGAGGATGG ATACGAGGTT CCCTCCAGCA GCAGCAAGGG CGATAAGAAG 
CGCTTTGAGG TGAAGAAGTG GAACGCCGTG GCTCTGTGGG CCTGGGACAT CGTGGTGGAC 
AACTGCGCCA TCTGCCGCAA CCACATCATG GACTTGTGCA TCGAGTGTCA GGCGAACCAG 
GCGTCCGCCA CTAGCGAGGA GTGCACCGTG GCCTGGGGCG TCTGCAACCA CGCCTTCCAT 
TTCCACTGCA TCTCTCGCTG GCTAAAGACG CGCCAGGTAT GCCCACTGGA CAACCGCGAG 
TGGGATTTCC AGAAGTACGG CCACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Roc1a ( NM_130496.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_569852.1 Drosophila melanogaster regulator of cullins 1a, transcript variant A (Roc1a), mRNA.

Price & Availability↑

CloneID	OFa00392
Clone ID Related Accession (Same CDS sequence)	NM_001297825.1 , NM_130496.4
Accession Version	NM_130496.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 327bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	regulator of cullins 1a, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_130496.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAAGTCG ACGAGGATGG ATACGAGGTT CCCTCCAGCA GCAGCAAGGG CGATAAGAAG 
CGCTTTGAGG TGAAGAAGTG GAACGCCGTG GCTCTGTGGG CCTGGGACAT CGTGGTGGAC 
AACTGCGCCA TCTGCCGCAA CCACATCATG GACTTGTGCA TCGAGTGTCA GGCGAACCAG 
GCGTCCGCCA CTAGCGAGGA GTGCACCGTG GCCTGGGGCG TCTGCAACCA CGCCTTCCAT 
TTCCACTGCA TCTCTCGCTG GCTAAAGACG CGCCAGGTAT GCCCACTGGA CAACCGCGAG 
TGGGATTTCC AGAAGTACGG CCACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_569852.1
CDS	145..471
Translation	MEVDEDGYEVPSSSSKGDKKRFEVKKWNAVALWAWDIVVDNCAICRNHIMDLCIECQANQASATSEECTVAWGVCNHAFHFHCISRWLKTRQVCPLDNREWDFQKYGH

Target ORF information:

RefSeq Version	NM_130496.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster regulator of cullins 1a, transcript variant A (Roc1a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_130496.4

ORF Insert Sequence:

ATGGAAGTCG ACGAGGATGG ATACGAGGTT CCCTCCAGCA GCAGCAAGGG CGATAAGAAG 
CGCTTTGAGG TGAAGAAGTG GAACGCCGTG GCTCTGTGGG CCTGGGACAT CGTGGTGGAC 
AACTGCGCCA TCTGCCGCAA CCACATCATG GACTTGTGCA TCGAGTGTCA GGCGAACCAG 
GCGTCCGCCA CTAGCGAGGA GTGCACCGTG GCCTGGGGCG TCTGCAACCA CGCCTTCCAT 
TTCCACTGCA TCTCTCGCTG GCTAAAGACG CGCCAGGTAT GCCCACTGGA CAACCGCGAG 
TGGGATTTCC AGAAGTACGG CCACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Roc1a cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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