G9a cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	G9a
Entrez Gene ID	30971
Full Name	CG2995 gene product from transcript CG2995-RB
Synonyms	CG2995,Dmel\CG2995,EG:BACR37P7.2,EHMT,cab65850 G9a Dm,dG9a
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 1A1-1A1

mRNA and Protein(s)

mRNA	Protein	Name
NM_130478.3	NP_569834.1	G9a, isoform A
NM_001272159.1	NP_001259088.1	G9a, isoform B

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

trr and G9a also have common direct targets, including the Drosophila ortholog of Arc (Arc1), a key regulator of synaptic plasticity. Our data highlight the clinical and molecular convergence between the KMT2 and EHMT protein families, which may contribute to a molecular network underlying a larger group of intellectual disability / autism spectrum disorder -related disorders.
Title: Functional convergence of histone methyltransferases EHMT1 and KMT2C involved in intellectual disability and autism spectrum disorder.

These data demonstrate a strong genetic link between dG9a and the EGFR signaling pathway.
Title: Genome-wide genetic screen identified the link between dG9a and epidermal growth factor receptor signaling pathway in vivo.

Authors show that the time required for hatching of a homozygous dG9a null mutant and heteroallelic combination of dG9a null mutants is delayed, suggesting that dG9a is at least partially responsible for progression of embryogenesis.
Title: Genomewide identification of target genes of histone methyltransferase dG9a during Drosophila embryogenesis.

G9a-deficient mutants are more sensitive to RNA virus infection and succumb faster to infection than wild-type controls
Title: The epigenetic regulator G9a mediates tolerance to RNA virus infection in Drosophila.

EHMT/G9a as a key regulator of cognition that orchestrates an epigenetic program featuring classic learning and memory genes
Title: Epigenetic regulation of learning and memory by Drosophila EHMT/G9a.

The following G9a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the G9a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa00336	NM_130478.3 Latest version!	Drosophila melanogaster G9a, transcript variant A (G9a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OFa03259	NM_001272159.1 Latest version!	Drosophila melanogaster G9a, transcript variant B (G9a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

G9a ( NM_130478.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_569834.1 Drosophila melanogaster G9a, transcript variant A (G9a), mRNA.

Price & Availability↑

CloneID	OFa00336
Clone ID Related Accession (Same CDS sequence)	NM_130478.3
Accession Version	NM_130478.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4914bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	G9a, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jan 16, 2013 this sequence version replaced NM_130478.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACGGACT TTGTTGAGCT GATGAACAGC ATGTCCAGTA CATTCAATAG CGACTGTGCT 
ACTTCAACAG CGGAGGGTGG TACTTTGCTA AATCTGAACT TGGCGGAAGA CAAAACCCTC 
AAGTGGCGTA ATTTGGCCAA TAACCAGTTC GCTAGTAAAG AAAAGAAGCA CAAGGACAAG 
GAGGAGGAGG AGCGCAAGGA GGCAAGGAAT CAAGAGGAAA TCGAAGACAT CAAAGCACTT 
TTGGCAGATG TTGTCGATGC GGCCGCTGTC AAGCTGGAAG AAGAAGAAGC GCAGAATGCA 
GAAAAAGTCG AACCACATAC AAAATGCGAA ATAGAGGAAG AAGGTAGGAA GGAAATGGAG 
TACGATCAAG ATGTCGCAAA ACAAGACTCC GAAATGGAAA AAAAGCAGAA TGGCAAAGCA 
ACATCCATAA CAGTAAAGAT GGAAAGCAAC GAGCGTGCAG AGAAACACGC AACGGAAATT 
GCAACGACGT CGACAGAGAG ATGGGAAAAC GAAAGCTTTA AAACTGAGCA GCAAAATAAA 
AAAGCTGCTG AGAAAGAAGA AGAACCCATA CTAGCAGCAA CACAAAAATT GGAAGCAAAT 
GCAGAACCAC TGACAACAAC AAGAATTGAG GTAGCAGTAG CATCTCCACT TGTTGTTAGT 
TCTGCTAGCG TTAAACTCGC TGCTGACGCA ACCAACCAAA TGCGAGCAGC GACGTCGGCA 
GGAGCAGCGA CATTAGCTGA TAAAAATGTA CAAGTTTCGC CCGGTGGAAC GCGACGGTCT 
CGAAGAACAC CCAGACCCAT AGACACGCCC ACTTCAGTTA CTGATGAACA TGTTCAGGTG 
GAGAATAAAA AATTTGGAAA GTCGGAGCAG TATACGGATT GTAGTAGCCA TCTGGAGAGG 
TTCACACTGG ACGATAATAC TGCTATTGTC AGACTGCAGT TGAAAAGTGA ACCGGACAAA 
CCGTCATTAA CTGCATTATC TCCTGAGGAA AACTCGGCTC CTGCTCCGAA GCGAGGAAGG 
GGTCGAGCCA GGAAAATAAG ACCCGATGCC GAGGTGGAAA CCAGTGAGGT TATTTTACCC 
TGCGAGGATT CATTAGGGGA GAAGAAACCA GGACGCAAAA GGAAACTCCC GGATGAACCG 
ATAGATCAGC AGCAACTAAG CGACCTTGTC GTGGTAAAAA CAGAGCAGGA GGAATTGGGT 
GACGCTCCCT TGGGAGATGT TAAGAGAATG CGACGTAGCG TTCGTTTAGG CAATCGATTA 
CACGCCGATG GAAGCCCTTG GGAGGAGGTA AAGACAGAGG CACTTCACCC GCAGCCTTCT 
GCAGAACTTT CCTTTGCGGA GGTCACTTCT GAGATCTTGC CACTAGCTGT CTTAGATGAG 
AAGACCCCAC CCAAAAAGCG AGGGCGGAAG GCAAAAACGC CCTGTGTGAA GTTAGAATCT 
GAAACATCCT GCGGGCTACC GTTTGCTAAT GGCAACAAGA AAACGAATTC CTCTGGTGGA 
TGCGAACTTC AGCTGCCCAA GCGTTCAAAA AGAAGAATAA AACCAACGCC AAAGATATTA 
GAAAATGACG AACTGCGCTG TGAGTTCGAA ACGAAGCACA TTGAGCGTAT GACCCAGTGG 
GAGAGTGCTG CAGCAGTAGA TGGGGATTTT GAAACGCCAA CAACAGGCGG TAATGGGTCC 
AATTCCTCGA CCTCAAGGCA AAAGAGCGAC AAGTCCGACG GGAGCAACTT CGAAGGTGGT 
CCAGGACATC CTGCAGGCAC AAGTGCCATA AAGAAAAGAC TATTTTCAAA GTCCCAGCGG 
GACATTGAGA ACTATGGCGC AGCAATGCTA GCCAAAAGTA AGTTACCTCC GTGTCCGGAT 
GTCGAACAAT TTCTAAACGA CATCAAGGCA TCTCGAATTA ACGCGAATCG GTCACCAGAA 
GAAAGGAAAT TGAACAAAAA ACAGCAACGA AAACTGGCCA AACAAAAGGA AAAGCACCTG 
AAGCATTTGG GCCTGCAAAA AAATCACAGA GATGAGCCCT CGGATAACGA CAGTTCCAAT 
ACGGACAATG AATTCTTTCC TACTACAAGG GTGCAAGTGG GAAAACCAAG TGTTACTTTG 
AGAGTAAGGA ATAGTGTTAC CAAGGAGCTT CCCACCACAG CCACTTTGAA ATCACGCCGG 
AATCCAGTCG TGCAGGCAGC CAAACTCACG AGAAGGATAG GTGCAAGAGC CGCCGGTGAA 
GTTACTGAGG CAGCCCGAGC AAGTGTGCCA ATTTCTACAC CTGATGCGGA GCAGCTTCAC 
TCGTTGGACA CTTCTATTCA AGCTGATGTT ACGCCCATAA GAGACTTAGA TATGAGGCCC 
AGCACCTCCA GAGTGTCAAA GTTTATTTGT CTATGTCAGA AGCCTTCCCA GTATTATGCC 
AGGAATGCCC CGGATTCATC ATACTGCTGT GCCATCGATC ACATCGACGA CCAAAAAATT 
GGCTGCTGCA ACGAGCTGTC CTCAGAGGTT CACAATCTAC TCAGACCCAG CCAACGCGTT 
AGCTATATGA TCCTATGCGA TGAGCACAAA AAGCGCCTAC AATCACACAA CTGCTGCGCA 
GGATGTGGCA TATTTTGTAC ACAGGGAAAA TTTGTGCTAT GCAAGCAGCA GCACTTCTTT 
CATCCCGATT GTGCCCAGCG ATTTATTCTA AGCACTTCGT ACGAGAAGGA ACTCGGCGAT 
GAGGAAGATC AGGGCGTCAA ATTCAGCAGC CCTGTCTTAG TGCTTAAGTG CCCCCACTGC 
GGATTAGATA CACCAGAACG GACCTCCACG GTGACCATGA AGTGTCAGTC CCTGCCTGTC 
TTTCTGCGCA CGCAAAAGTA CAAGATCAAA CCCGCTAGAT TGACAACCTC TTCGCATCTA 
ACCCAATTTG GAACAGTTGA GAATGCCAAT ACCCCAGGAG CCACCGCCAG GAACAAGGGA 
GGACTATCCA CCGCCGTAAC CTTAAGCGCG GCATCAAGTC CTGCCTCAAA AACAAATGGA 
GCCCAGAGAG GCAGGGCGGG CACCTCCAAT TCTAACTCCA GGCATGCTCT TAATTCGATC 
AACTTTGCTC AACTTATACC CGAGTCCGTA ATGAATGTAG TGCTGCGGGG CCACGTGGTT 
TCTGCTAGCG GTCGCGTGAC CGCAGAGTTT ACCCCGCGGG ATATGTACTA TGCAGTCCAA 
AACGATGACT TGGAGCGTGT AGCGGAGATT CTGGCTGCCG ACTTCAACGT GCTGACTCCC 
ATACGGGAGT ACTTAAATGG CACCTGCCTT CATTTGGTGG CCCACTCGGG CACACTTCAG 
ATGGCCTATC TCCTTCTCTG CAAAGGAGCA AGCTCACCGG ATTTCGTTAA TATTGTGGAT 
TATGAACTGC GGACTGCTTT AATGTGTGCC GTGATGAACG AGAAGTGCGA CATGCTGAAT 
CTATTTCTTC AATGTGGTGC GGATGTGGCC ATTAAGGGTC CCGATGGTAA GACCAGTCTG 
CATATTGCTG CCCAGTTAGG CAACTTGGAA GCCACACAGT TAATTGTAGA CAGCTATCGA 
ACTTCTCGAA ATATTACCAG TTTTTTAAGT TTCATTGATG CCCAGGATGA AGGCGGTTGG 
ACGGCCATGG TCTGGGCAGC AGAGCTGGGG CACACGGATA TAGTTAGCTT CCTGCTTAAC 
CAGGATGCTG ATCCAAACAT ATGCGACAAT GACAACAATA CGGTGCTGCA CTGGTCTACG 
CTGCACAATG ATGGTCTGGA CACGATTACA GTACTGCTGC AGTCAGGGGC GGACTGCAAT 
GTACAGAATG TAGAGGGCGA CACGCCTTTG CACATTGCTT GCCGGCATTC TGTCACCCGC 
ATGTGCATCG CGTTAATAGC TAATGGAGCC GATCTAATGA TTAAGAACAA GGCGGAACAA 
CTGCCCTTTG ATTGCATTCC GAATGAGGAG TCTGAGTGCG GTCGCACTGT AGGTTTTAAT 
ATGCAAATGC GTAGTTTCAG GCCTTTGGGC CTCCGTACCT TTGTGGTTTG TGCAGATGCG 
TCGAATGGCA GGGAGGCACG ACCAATCCAG GTGGTTCGCA ACGAACTGGC AATGTCTGAA 
AATGAGGATG AGGCCGATTC GCTTATGTGG CCGGATTTTC GCTACGTAAC ACAATGCATT 
ATCCAGCAGA ATTCAGTGCA GATTGACCGA CGCGTTTCTC AAATGCGCAT CTGCTCCTGC 
CTGGATAGTT GCAGTAGCGA TCGCTGTCAG TGCAATGGAG CCTCCTCGCA GAATTGGTAC 
ACGGCAGAAA GTCGGCTAAA CGCCGACTTT AACTACGAGG ATCCTGCGGT AATCTTCGAA 
TGCAACGACG TCTGCGGTTG CAACCAGCTG TCCTGCAAAA ACCGCGTGGT GCAGAATGGT 
ACACGGACAC CCCTACAAAT TGTCGAGTGC GAGGATCAGG CGAAGGGCTG GGGAGTACGG 
GCGCTGGCCA ATGTACCCAA AGGCACTTTT GTGGGCAGTT ATACGGGTGA GATTTTGACC 
GCGATGGAGG CAGATCGACG CACCGATGAC AGCTACTACT TTGACCTGGA CAACGGGCAC 
TGCATCGATG CCAACTATTA TGGAAATGTA ACCCGGTTTT TTAACCACTC GTGTGAGCCG 
AATGTTTTGC CCGTTCGTGT TTTCTACGAG CATCAGGACT ACAGATTCCC TAAGATCGCC 
TTTTTTTCCT GCCGTGACAT CGATGCCGGA GAGGAAATAT GCTTTGACTA CGGAGAAAAA 
TTCTGGCGCG TGGAACATCG CTCCTGTGTT GGATGTCGAT GCCTGACAAC AACCTGCAAG 
TACGCCTCCC AATCCTCAAG CACAAATGCC TCGCCTACGA ATGCAACAAC GGCACCGGAA 
AATGAAACGG GAACGCTGTC GTCTACAAAT ACGGAGAAAA TTGGACACGC GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_569834.1
CDS	873..5786
Translation	MTDFVELMNSMSSTFNSDCATSTAEGGTLLNLNLAEDKTLKWRNLANNQFASKEKKHKDKEEEERKEARNQEEIEDIKALLADVVDAAAVKLEEEEAQNAEKVEPHTKCEIEEEGRKEMEYDQDVAKQDSEMEKKQNGKATSITVKMESNERAEKHATEIATTSTERWENESFKTEQQNKKAAEKEEEPILAATQKLEANAEPLTTTRIEVAVASPLVVSSASVKLAADATNQMRAATSAGAATLADKNVQVSPGGTRRSRRTPRPIDTPTSVTDEHVQVENKKFGKSEQYTDCSSHLERFTLDDNTAIVRLQLKSEPDKPSLTALSPEENSAPAPKRGRGRARKIRPDAEVETSEVILPCEDSLGEKKPGRKRKLPDEPIDQQQLSDLVVVKTEQEELGDAPLGDVKRMRRSVRLGNRLHADGSPWEEVKTEALHPQPSAELSFAEVTSEILPLAVLDEKTPPKKRGRKAKTPCVKLESETSCGLPFANGNKKTNSSGGCELQLPKRSKRRIKPTPKILENDELRCEFETKHIERMTQWESAAAVDGDFETPTTGGNGSNSSTSRQKSDKSDGSNFEGGPGHPAGTSAIKKRLFSKSQRDIENYGAAMLAKSKLPPCPDVEQFLNDIKASRINANRSPEERKLNKKQQRKLAKQKEKHLKHLGLQKNHRDEPSDNDSSNTDNEFFPTTRVQVGKPSVTLRVRNSVTKELPTTATLKSRRNPVVQAAKLTRRIGARAAGEVTEAARASVPISTPDAEQLHSLDTSIQADVTPIRDLDMRPSTSRVSKFICLCQKPSQYYARNAPDSSYCCAIDHIDDQKIGCCNELSSEVHNLLRPSQRVSYMILCDEHKKRLQSHNCCAGCGIFCTQGKFVLCKQQHFFHPDCAQRFILSTSYEKELGDEEDQGVKFSSPVLVLKCPHCGLDTPERTSTVTMKCQSLPVFLRTQKYKIKPARLTTSSHLTQFGTVENANTPGATARNKGGLSTAVTLSAASSPASKTNGAQRGRAGTSNSNSRHALNSINFAQLIPESVMNVVLRGHVVSASGRVTAEFTPRDMYYAVQNDDLERVAEILAADFNVLTPIREYLNGTCLHLVAHSGTLQMAYLLLCKGASSPDFVNIVDYELRTALMCAVMNEKCDMLNLFLQCGADVAIKGPDGKTSLHIAAQLGNLEATQLIVDSYRTSRNITSFLSFIDAQDEGGWTAMVWAAELGHTDIVSFLLNQDADPNICDNDNNTVLHWSTLHNDGLDTITVLLQSGADCNVQNVEGDTPLHIACRHSVTRMCIALIANGADLMIKNKAEQLPFDCIPNEESECGRTVGFNMQMRSFRPLGLRTFVVCADASNGREARPIQVVRNELAMSENEDEADSLMWPDFRYVTQCIIQQNSVQIDRRVSQMRICSCLDSCSSDRCQCNGASSQNWYTAESRLNADFNYEDPAVIFECNDVCGCNQLSCKNRVVQNGTRTPLQIVECEDQAKGWGVRALANVPKGTFVGSYTGEILTAMEADRRTDDSYYFDLDNGHCIDANYYGNVTRFFNHSCEPNVLPVRVFYEHQDYRFPKIAFFSCRDIDAGEEICFDYGEKFWRVEHRSCVGCRCLTTTCKYASQSSSTNASPTNATTAPENETGTLSSTNTEKIGHA

Target ORF information:

RefSeq Version	NM_130478.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster G9a, transcript variant A (G9a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_130478.3

ORF Insert Sequence:

ATGACGGACT TTGTTGAGCT GATGAACAGC ATGTCCAGTA CATTCAATAG CGACTGTGCT 
ACTTCAACAG CGGAGGGTGG TACTTTGCTA AATCTGAACT TGGCGGAAGA CAAAACCCTC 
AAGTGGCGTA ATTTGGCCAA TAACCAGTTC GCTAGTAAAG AAAAGAAGCA CAAGGACAAG 
GAGGAGGAGG AGCGCAAGGA GGCAAGGAAT CAAGAGGAAA TCGAAGACAT CAAAGCACTT 
TTGGCAGATG TTGTCGATGC GGCCGCTGTC AAGCTGGAAG AAGAAGAAGC GCAGAATGCA 
GAAAAAGTCG AACCACATAC AAAATGCGAA ATAGAGGAAG AAGGTAGGAA GGAAATGGAG 
TACGATCAAG ATGTCGCAAA ACAAGACTCC GAAATGGAAA AAAAGCAGAA TGGCAAAGCA 
ACATCCATAA CAGTAAAGAT GGAAAGCAAC GAGCGTGCAG AGAAACACGC AACGGAAATT 
GCAACGACGT CGACAGAGAG ATGGGAAAAC GAAAGCTTTA AAACTGAGCA GCAAAATAAA 
AAAGCTGCTG AGAAAGAAGA AGAACCCATA CTAGCAGCAA CACAAAAATT GGAAGCAAAT 
GCAGAACCAC TGACAACAAC AAGAATTGAG GTAGCAGTAG CATCTCCACT TGTTGTTAGT 
TCTGCTAGCG TTAAACTCGC TGCTGACGCA ACCAACCAAA TGCGAGCAGC GACGTCGGCA 
GGAGCAGCGA CATTAGCTGA TAAAAATGTA CAAGTTTCGC CCGGTGGAAC GCGACGGTCT 
CGAAGAACAC CCAGACCCAT AGACACGCCC ACTTCAGTTA CTGATGAACA TGTTCAGGTG 
GAGAATAAAA AATTTGGAAA GTCGGAGCAG TATACGGATT GTAGTAGCCA TCTGGAGAGG 
TTCACACTGG ACGATAATAC TGCTATTGTC AGACTGCAGT TGAAAAGTGA ACCGGACAAA 
CCGTCATTAA CTGCATTATC TCCTGAGGAA AACTCGGCTC CTGCTCCGAA GCGAGGAAGG 
GGTCGAGCCA GGAAAATAAG ACCCGATGCC GAGGTGGAAA CCAGTGAGGT TATTTTACCC 
TGCGAGGATT CATTAGGGGA GAAGAAACCA GGACGCAAAA GGAAACTCCC GGATGAACCG 
ATAGATCAGC AGCAACTAAG CGACCTTGTC GTGGTAAAAA CAGAGCAGGA GGAATTGGGT 
GACGCTCCCT TGGGAGATGT TAAGAGAATG CGACGTAGCG TTCGTTTAGG CAATCGATTA 
CACGCCGATG GAAGCCCTTG GGAGGAGGTA AAGACAGAGG CACTTCACCC GCAGCCTTCT 
GCAGAACTTT CCTTTGCGGA GGTCACTTCT GAGATCTTGC CACTAGCTGT CTTAGATGAG 
AAGACCCCAC CCAAAAAGCG AGGGCGGAAG GCAAAAACGC CCTGTGTGAA GTTAGAATCT 
GAAACATCCT GCGGGCTACC GTTTGCTAAT GGCAACAAGA AAACGAATTC CTCTGGTGGA 
TGCGAACTTC AGCTGCCCAA GCGTTCAAAA AGAAGAATAA AACCAACGCC AAAGATATTA 
GAAAATGACG AACTGCGCTG TGAGTTCGAA ACGAAGCACA TTGAGCGTAT GACCCAGTGG 
GAGAGTGCTG CAGCAGTAGA TGGGGATTTT GAAACGCCAA CAACAGGCGG TAATGGGTCC 
AATTCCTCGA CCTCAAGGCA AAAGAGCGAC AAGTCCGACG GGAGCAACTT CGAAGGTGGT 
CCAGGACATC CTGCAGGCAC AAGTGCCATA AAGAAAAGAC TATTTTCAAA GTCCCAGCGG 
GACATTGAGA ACTATGGCGC AGCAATGCTA GCCAAAAGTA AGTTACCTCC GTGTCCGGAT 
GTCGAACAAT TTCTAAACGA CATCAAGGCA TCTCGAATTA ACGCGAATCG GTCACCAGAA 
GAAAGGAAAT TGAACAAAAA ACAGCAACGA AAACTGGCCA AACAAAAGGA AAAGCACCTG 
AAGCATTTGG GCCTGCAAAA AAATCACAGA GATGAGCCCT CGGATAACGA CAGTTCCAAT 
ACGGACAATG AATTCTTTCC TACTACAAGG GTGCAAGTGG GAAAACCAAG TGTTACTTTG 
AGAGTAAGGA ATAGTGTTAC CAAGGAGCTT CCCACCACAG CCACTTTGAA ATCACGCCGG 
AATCCAGTCG TGCAGGCAGC CAAACTCACG AGAAGGATAG GTGCAAGAGC CGCCGGTGAA 
GTTACTGAGG CAGCCCGAGC AAGTGTGCCA ATTTCTACAC CTGATGCGGA GCAGCTTCAC 
TCGTTGGACA CTTCTATTCA AGCTGATGTT ACGCCCATAA GAGACTTAGA TATGAGGCCC 
AGCACCTCCA GAGTGTCAAA GTTTATTTGT CTATGTCAGA AGCCTTCCCA GTATTATGCC 
AGGAATGCCC CGGATTCATC ATACTGCTGT GCCATCGATC ACATCGACGA CCAAAAAATT 
GGCTGCTGCA ACGAGCTGTC CTCAGAGGTT CACAATCTAC TCAGACCCAG CCAACGCGTT 
AGCTATATGA TCCTATGCGA TGAGCACAAA AAGCGCCTAC AATCACACAA CTGCTGCGCA 
GGATGTGGCA TATTTTGTAC ACAGGGAAAA TTTGTGCTAT GCAAGCAGCA GCACTTCTTT 
CATCCCGATT GTGCCCAGCG ATTTATTCTA AGCACTTCGT ACGAGAAGGA ACTCGGCGAT 
GAGGAAGATC AGGGCGTCAA ATTCAGCAGC CCTGTCTTAG TGCTTAAGTG CCCCCACTGC 
GGATTAGATA CACCAGAACG GACCTCCACG GTGACCATGA AGTGTCAGTC CCTGCCTGTC 
TTTCTGCGCA CGCAAAAGTA CAAGATCAAA CCCGCTAGAT TGACAACCTC TTCGCATCTA 
ACCCAATTTG GAACAGTTGA GAATGCCAAT ACCCCAGGAG CCACCGCCAG GAACAAGGGA 
GGACTATCCA CCGCCGTAAC CTTAAGCGCG GCATCAAGTC CTGCCTCAAA AACAAATGGA 
GCCCAGAGAG GCAGGGCGGG CACCTCCAAT TCTAACTCCA GGCATGCTCT TAATTCGATC 
AACTTTGCTC AACTTATACC CGAGTCCGTA ATGAATGTAG TGCTGCGGGG CCACGTGGTT 
TCTGCTAGCG GTCGCGTGAC CGCAGAGTTT ACCCCGCGGG ATATGTACTA TGCAGTCCAA 
AACGATGACT TGGAGCGTGT AGCGGAGATT CTGGCTGCCG ACTTCAACGT GCTGACTCCC 
ATACGGGAGT ACTTAAATGG CACCTGCCTT CATTTGGTGG CCCACTCGGG CACACTTCAG 
ATGGCCTATC TCCTTCTCTG CAAAGGAGCA AGCTCACCGG ATTTCGTTAA TATTGTGGAT 
TATGAACTGC GGACTGCTTT AATGTGTGCC GTGATGAACG AGAAGTGCGA CATGCTGAAT 
CTATTTCTTC AATGTGGTGC GGATGTGGCC ATTAAGGGTC CCGATGGTAA GACCAGTCTG 
CATATTGCTG CCCAGTTAGG CAACTTGGAA GCCACACAGT TAATTGTAGA CAGCTATCGA 
ACTTCTCGAA ATATTACCAG TTTTTTAAGT TTCATTGATG CCCAGGATGA AGGCGGTTGG 
ACGGCCATGG TCTGGGCAGC AGAGCTGGGG CACACGGATA TAGTTAGCTT CCTGCTTAAC 
CAGGATGCTG ATCCAAACAT ATGCGACAAT GACAACAATA CGGTGCTGCA CTGGTCTACG 
CTGCACAATG ATGGTCTGGA CACGATTACA GTACTGCTGC AGTCAGGGGC GGACTGCAAT 
GTACAGAATG TAGAGGGCGA CACGCCTTTG CACATTGCTT GCCGGCATTC TGTCACCCGC 
ATGTGCATCG CGTTAATAGC TAATGGAGCC GATCTAATGA TTAAGAACAA GGCGGAACAA 
CTGCCCTTTG ATTGCATTCC GAATGAGGAG TCTGAGTGCG GTCGCACTGT AGGTTTTAAT 
ATGCAAATGC GTAGTTTCAG GCCTTTGGGC CTCCGTACCT TTGTGGTTTG TGCAGATGCG 
TCGAATGGCA GGGAGGCACG ACCAATCCAG GTGGTTCGCA ACGAACTGGC AATGTCTGAA 
AATGAGGATG AGGCCGATTC GCTTATGTGG CCGGATTTTC GCTACGTAAC ACAATGCATT 
ATCCAGCAGA ATTCAGTGCA GATTGACCGA CGCGTTTCTC AAATGCGCAT CTGCTCCTGC 
CTGGATAGTT GCAGTAGCGA TCGCTGTCAG TGCAATGGAG CCTCCTCGCA GAATTGGTAC 
ACGGCAGAAA GTCGGCTAAA CGCCGACTTT AACTACGAGG ATCCTGCGGT AATCTTCGAA 
TGCAACGACG TCTGCGGTTG CAACCAGCTG TCCTGCAAAA ACCGCGTGGT GCAGAATGGT 
ACACGGACAC CCCTACAAAT TGTCGAGTGC GAGGATCAGG CGAAGGGCTG GGGAGTACGG 
GCGCTGGCCA ATGTACCCAA AGGCACTTTT GTGGGCAGTT ATACGGGTGA GATTTTGACC 
GCGATGGAGG CAGATCGACG CACCGATGAC AGCTACTACT TTGACCTGGA CAACGGGCAC 
TGCATCGATG CCAACTATTA TGGAAATGTA ACCCGGTTTT TTAACCACTC GTGTGAGCCG 
AATGTTTTGC CCGTTCGTGT TTTCTACGAG CATCAGGACT ACAGATTCCC TAAGATCGCC 
TTTTTTTCCT GCCGTGACAT CGATGCCGGA GAGGAAATAT GCTTTGACTA CGGAGAAAAA 
TTCTGGCGCG TGGAACATCG CTCCTGTGTT GGATGTCGAT GCCTGACAAC AACCTGCAAG 
TACGCCTCCC AATCCTCAAG CACAAATGCC TCGCCTACGA ATGCAACAAC GGCACCGGAA 
AATGAAACGG GAACGCTGTC GTCTACAAAT ACGGAGAAAA TTGGACACGC GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

G9a ( NM_001272159.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001259088.1 Drosophila melanogaster G9a, transcript variant B (G9a), mRNA.

Price & Availability↑

CloneID	OFa03259
Clone ID Related Accession (Same CDS sequence)	NM_001272159.1
Accession Version	NM_001272159.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4974bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	G9a, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACGGACT TTGTTGAGCT GATGAACAGC ATGTCCAGTA CATTCAATAG CGACTGTGCT 
ACTTCAACAG CGGAGGGTGG TACTTTGCTA AATCTGAACT TGGCGGAAGA CAAAACCCTC 
AAGTGGCGTA ATTTGGCCAA TAACCAGTTC GCTAGTAAAG AAAAGAAGCA CAAGGACAAG 
GAGGAGGAGG AGCGCAAGGA GGCAAGGAAT CAAGAGGAAA TCGAAGACAT CAAAGCACTT 
TTGGCAGATG TTGTCGATGC GGCCGCTGTC AAGCTGGAAG AAGAAGAAGC GCAGAATGCA 
GAAAAAGTCG AACCACATAC AAAATGCGAA ATAGAGGAAG AAGGTAGGAA GGAAATGGAG 
TACGATCAAG ATGTCGCAAA ACAAGACTCC GAAATGGAAA AAAAGCAGAA TGGCAAAGCA 
ACATCCATAA CAGTAAAGAT GGAAAGCAAC GAGCGTGCAG AGAAACACGC AACGGAAATT 
GCAACGACGT CGACAGAGAG ATGGGAAAAC GAAAGCTTTA AAACTGAGCA GCAAAATAAA 
AAAGCTGCTG AGAAAGAAGA AGAACCCATA CTAGCAGCAA CACAAAAATT GGAAGCAAAT 
GCAGAACCAC TGACAACAAC AAGAATTGAG GTAGCAGTAG CATCTCCACT TGTTGTTAGT 
TCTGCTAGCG TTAAACTCGC TGCTGACGCA ACCAACCAAA TGCGAGCAGC GACGTCGGCA 
GGAGCAGCGA CATTAGCTGA TAAAAATGTA CAAGTTTCGC CCGGTGGAAC GCGACGGTCT 
CGAAGAACAC CCAGACCCAT AGACACGCCC ACTTCAGTTA CTGATGAACA TGTTCAGGTG 
GAGAATAAAA AATTTGGAAA GTCGGAGCAG TATACGGATT GTAGTAGCCA TCTGGAGAGG 
TTCACACTGG ACGATAATAC TGCTATTGTC AGACTGCAGT TGAAAAGTGA ACCGGACAAA 
CCGTCATTAA CTGCATTATC TCCTGAGGAA AACTCGGCTC CTGCTCCGAA GCGAGGAAGG 
GGTCGAGCCA GGAAAATAAG ACCCGATGCC GAGGTGGAAA CCAGTGAGGT TATTTTACCC 
TGCGAGGATT CATTAGGGGA GAAGAAACCA GGACGCAAAA GGAAACTCCC GGATGAACCG 
ATAGATCAGC AGCAACTAAG CGACCTTGTC GTGGTAAAAA CAGAGCAGGA GGAATTGGGT 
GACGCTCCCT TGGGAGATGT TAAGAGAATG CGACGTAGCG TTCGTTTAGG CAATCGATTA 
CACGCCGATG GAAGCCCTTG GGAGGAGGTA AAGACAGAGG CACTTCACCC GCAGCCTTCT 
GCAGAACTTT CCTTTGCGGA GGTCACTTCT GAGATCTTGC CACTAGCTGT CTTAGATGAG 
AAGACCCCAC CCAAAAAGCG AGGGCGGAAG GCAAAAACGC CCTGTGTGAA GTTAGAATCT 
GAAACATCCT GCGGGCTACC GTTTGCTAAT GGCAACAAGA AAACGAATTC CTCTGGTGGA 
TGCGAACTTC AGCTGCCCAA GCGTTCAAAA AGAAGAATAA AACCAACGCC AAAGATATTA 
GAAAATGACG AACTGCGCTG TGAGTTCGAA ACGAAGCACA TTGAGCGTAT GACCCAGTGG 
GAGAGTGCTG CAGCAGTAGA TGGGGATTTT GAAACGCCAA CAACAGGCGG TAATGGGTCC 
AATTCCTCGA CCTCAAGGCA AAAGAGCGAC AAGTCCGACG GGAGCAACTT CGAAGGTGGT 
CCAGGACATC CTGCAGGCAC AAGTGCCATA AAGAAAAGAC TATTTTCAAA GTCCCAGCGG 
GACATTGAGA ACTATGGCGC AGCAATGCTA GCCAAAAGTA AGTTACCTCC GTGTCCGGAT 
GTCGAACAAT TTCTAAACGA CATCAAGGCA TCTCGAATTA ACGCGAATCG GTCACCAGAA 
GAAAGGAAAT TGAACAAAAA ACAGCAACGA AAACTGGCCA AACAAAAGGA AAAGCACCTG 
AAGCATTTGG GCCTGCAAAA AAATCACAGA GATGAGCCCT CGGATAACGA CAGTTCCAAT 
ACGGACAATG AATTCTTTCC TACTACAAGG GTGCAAGTGG GAAAACCAAG TGTTACTTTG 
AGAGTAAGGA ATAGTGTTAC CAAGGAGCTT CCCACCACAG CCACTTTGAA ATCACGCCGG 
AATCCAGTCG TGCAGGCAGC CAAACTCACG AGAAGGATAG GTGCAAGAGC CGCCGGTGAA 
GTTACTGAGG CAGCCCGAGC AAGTGTGCCA ATTTCTACAC CTGATGCGGA GCAGCTTCAC 
TCGTTGGACA CTTCTATTCA AGCTGATGTT ACGCCCATAA GAGACTTAGA TATGAGGCCC 
AGCACCTCCA GAGTGTCAAA GTTTATTTGT CTATGTCAGA AGCCTTCCCA GTATTATGCC 
AGGAATGCCC CGGATTCATC ATACTGCTGT GCCATCGATC ACATCGACGA CCAAAAAATT 
GGCTGCTGCA ACGAGCTGTC CTCAGAGGTT CACAATCTAC TCAGACCCAG CCAACGCGTT 
AGCTATATGA TCCTATGCGA TGAGCACAAA AAGCGCCTAC AATCACACAA CTGCTGCGCA 
GGATGTGGCA TATTTTGTAC ACAGGGAAAA TTTGTGCTAT GCAAGCAGCA GCACTTCTTT 
CATCCCGATT GTGCCCAGCG ATTTATTCTA AGCACTTCGT ACGAGAAGGA ACTCGGCGAT 
GAGGAAGATC AGGGCGTCAA ATTCAGCAGC CCTGTCTTAG TGCTTAAGTG CCCCCACTGC 
GGATTAGATA CACCAGAACG GACCTCCACG GTGACCATGA AGTGTCAGTC CCTGCCTGTC 
TTTCTGCGCA CGCAAAAGTA CAAGATCAAA CCCGCTAGAT TGACAACCTC TTCGCATCTA 
ACCCAATTTG GAACAGTTGA GAATGCCAAT ACCCCAGGAG CCACCGCCAG GAACAAGGGA 
GGACTATCCA CCGCCGTAAC CTTAAGCGCG GCATCAAGTC CTGCCTCAAA AACAAATGGA 
GCCCAGAGAG GCAGGGCGGG CACCTCCAAT TCTAACTCCA GGCATGCTCT TAATTCGATC 
AACTTTGCTC AACTTATACC CGAGTCCGTA ATGAATGTAG TGCTGCGGGG CCACGTGGTT 
TCTGCTAGCG GTCGCGTGAC CGCAGAGTTT ACCCCGCGGG ATATGTACTA TGCAGTCCAA 
AACGATGACT TGGAGCGTGT AGCGGAGATT CTGGCTGCCG ACTTCAACGT GCTGACTCCC 
ATACGGGAGT ACTTAAATGG CACCTGCCTT CATTTGGTGG CCCACTCGGG CACACTTCAG 
ATGGCCTATC TCCTTCTCTG CAAAGGAGCA AGCTCACCGG ATTTCGTTAA TATTGTGGAT 
TATGAACTGC GGACTGCTTT AATGTGTGCC GTGATGAACG AGAAGTGCGA CATGCTGAAT 
CTATTTCTTC AATGTGGTGC GGATGTGGCC ATTAAGGGTC CCGATGGTAA GACCAGTCTG 
CATATTGCTG CCCAGTTAGG CAACTTGGAA GCCACACAGT TAATTGTAGA CAGCTATCGA 
ACTTCTCGAA ATATTACCAG TTTTTTAAGT TTCATTGATG CCCAGGATGA AGGCGGTTGG 
ACGGCCATGG TCTGGGCAGC AGAGCTGGGG CACACGGATA TAGTTAGGTT GGCTTCTCTG 
CCCCAAGCGG TTTTCTTAAA GCTAATTAAT ATTTTTCTCT TCATTAGCTT CCTGCTTAAC 
CAGGATGCTG ATCCAAACAT ATGCGACAAT GACAACAATA CGGTGCTGCA CTGGTCTACG 
CTGCACAATG ATGGTCTGGA CACGATTACA GTACTGCTGC AGTCAGGGGC GGACTGCAAT 
GTACAGAATG TAGAGGGCGA CACGCCTTTG CACATTGCTT GCCGGCATTC TGTCACCCGC 
ATGTGCATCG CGTTAATAGC TAATGGAGCC GATCTAATGA TTAAGAACAA GGCGGAACAA 
CTGCCCTTTG ATTGCATTCC GAATGAGGAG TCTGAGTGCG GTCGCACTGT AGGTTTTAAT 
ATGCAAATGC GTAGTTTCAG GCCTTTGGGC CTCCGTACCT TTGTGGTTTG TGCAGATGCG 
TCGAATGGCA GGGAGGCACG ACCAATCCAG GTGGTTCGCA ACGAACTGGC AATGTCTGAA 
AATGAGGATG AGGCCGATTC GCTTATGTGG CCGGATTTTC GCTACGTAAC ACAATGCATT 
ATCCAGCAGA ATTCAGTGCA GATTGACCGA CGCGTTTCTC AAATGCGCAT CTGCTCCTGC 
CTGGATAGTT GCAGTAGCGA TCGCTGTCAG TGCAATGGAG CCTCCTCGCA GAATTGGTAC 
ACGGCAGAAA GTCGGCTAAA CGCCGACTTT AACTACGAGG ATCCTGCGGT AATCTTCGAA 
TGCAACGACG TCTGCGGTTG CAACCAGCTG TCCTGCAAAA ACCGCGTGGT GCAGAATGGT 
ACACGGACAC CCCTACAAAT TGTCGAGTGC GAGGATCAGG CGAAGGGCTG GGGAGTACGG 
GCGCTGGCCA ATGTACCCAA AGGCACTTTT GTGGGCAGTT ATACGGGTGA GATTTTGACC 
GCGATGGAGG CAGATCGACG CACCGATGAC AGCTACTACT TTGACCTGGA CAACGGGCAC 
TGCATCGATG CCAACTATTA TGGAAATGTA ACCCGGTTTT TTAACCACTC GTGTGAGCCG 
AATGTTTTGC CCGTTCGTGT TTTCTACGAG CATCAGGACT ACAGATTCCC TAAGATCGCC 
TTTTTTTCCT GCCGTGACAT CGATGCCGGA GAGGAAATAT GCTTTGACTA CGGAGAAAAA 
TTCTGGCGCG TGGAACATCG CTCCTGTGTT GGATGTCGAT GCCTGACAAC AACCTGCAAG 
TACGCCTCCC AATCCTCAAG CACAAATGCC TCGCCTACGA ATGCAACAAC GGCACCGGAA 
AATGAAACGG GAACGCTGTC GTCTACAAAT ACGGAGAAAA TTGGACACGC GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001259088.1
CDS	873..5846
Translation	MTDFVELMNSMSSTFNSDCATSTAEGGTLLNLNLAEDKTLKWRNLANNQFASKEKKHKDKEEEERKEARNQEEIEDIKALLADVVDAAAVKLEEEEAQNAEKVEPHTKCEIEEEGRKEMEYDQDVAKQDSEMEKKQNGKATSITVKMESNERAEKHATEIATTSTERWENESFKTEQQNKKAAEKEEEPILAATQKLEANAEPLTTTRIEVAVASPLVVSSASVKLAADATNQMRAATSAGAATLADKNVQVSPGGTRRSRRTPRPIDTPTSVTDEHVQVENKKFGKSEQYTDCSSHLERFTLDDNTAIVRLQLKSEPDKPSLTALSPEENSAPAPKRGRGRARKIRPDAEVETSEVILPCEDSLGEKKPGRKRKLPDEPIDQQQLSDLVVVKTEQEELGDAPLGDVKRMRRSVRLGNRLHADGSPWEEVKTEALHPQPSAELSFAEVTSEILPLAVLDEKTPPKKRGRKAKTPCVKLESETSCGLPFANGNKKTNSSGGCELQLPKRSKRRIKPTPKILENDELRCEFETKHIERMTQWESAAAVDGDFETPTTGGNGSNSSTSRQKSDKSDGSNFEGGPGHPAGTSAIKKRLFSKSQRDIENYGAAMLAKSKLPPCPDVEQFLNDIKASRINANRSPEERKLNKKQQRKLAKQKEKHLKHLGLQKNHRDEPSDNDSSNTDNEFFPTTRVQVGKPSVTLRVRNSVTKELPTTATLKSRRNPVVQAAKLTRRIGARAAGEVTEAARASVPISTPDAEQLHSLDTSIQADVTPIRDLDMRPSTSRVSKFICLCQKPSQYYARNAPDSSYCCAIDHIDDQKIGCCNELSSEVHNLLRPSQRVSYMILCDEHKKRLQSHNCCAGCGIFCTQGKFVLCKQQHFFHPDCAQRFILSTSYEKELGDEEDQGVKFSSPVLVLKCPHCGLDTPERTSTVTMKCQSLPVFLRTQKYKIKPARLTTSSHLTQFGTVENANTPGATARNKGGLSTAVTLSAASSPASKTNGAQRGRAGTSNSNSRHALNSINFAQLIPESVMNVVLRGHVVSASGRVTAEFTPRDMYYAVQNDDLERVAEILAADFNVLTPIREYLNGTCLHLVAHSGTLQMAYLLLCKGASSPDFVNIVDYELRTALMCAVMNEKCDMLNLFLQCGADVAIKGPDGKTSLHIAAQLGNLEATQLIVDSYRTSRNITSFLSFIDAQDEGGWTAMVWAAELGHTDIVRLASLPQAVFLKLINIFLFISFLLNQDADPNICDNDNNTVLHWSTLHNDGLDTITVLLQSGADCNVQNVEGDTPLHIACRHSVTRMCIALIANGADLMIKNKAEQLPFDCIPNEESECGRTVGFNMQMRSFRPLGLRTFVVCADASNGREARPIQVVRNELAMSENEDEADSLMWPDFRYVTQCIIQQNSVQIDRRVSQMRICSCLDSCSSDRCQCNGASSQNWYTAESRLNADFNYEDPAVIFECNDVCGCNQLSCKNRVVQNGTRTPLQIVECEDQAKGWGVRALANVPKGTFVGSYTGEILTAMEADRRTDDSYYFDLDNGHCIDANYYGNVTRFFNHSCEPNVLPVRVFYEHQDYRFPKIAFFSCRDIDAGEEICFDYGEKFWRVEHRSCVGCRCLTTTCKYASQSSSTNASPTNATTAPENETGTLSSTNTEKIGHA

Target ORF information:

RefSeq Version	NM_001272159.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster G9a, transcript variant B (G9a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001272159.1

ORF Insert Sequence:

ATGACGGACT TTGTTGAGCT GATGAACAGC ATGTCCAGTA CATTCAATAG CGACTGTGCT 
ACTTCAACAG CGGAGGGTGG TACTTTGCTA AATCTGAACT TGGCGGAAGA CAAAACCCTC 
AAGTGGCGTA ATTTGGCCAA TAACCAGTTC GCTAGTAAAG AAAAGAAGCA CAAGGACAAG 
GAGGAGGAGG AGCGCAAGGA GGCAAGGAAT CAAGAGGAAA TCGAAGACAT CAAAGCACTT 
TTGGCAGATG TTGTCGATGC GGCCGCTGTC AAGCTGGAAG AAGAAGAAGC GCAGAATGCA 
GAAAAAGTCG AACCACATAC AAAATGCGAA ATAGAGGAAG AAGGTAGGAA GGAAATGGAG 
TACGATCAAG ATGTCGCAAA ACAAGACTCC GAAATGGAAA AAAAGCAGAA TGGCAAAGCA 
ACATCCATAA CAGTAAAGAT GGAAAGCAAC GAGCGTGCAG AGAAACACGC AACGGAAATT 
GCAACGACGT CGACAGAGAG ATGGGAAAAC GAAAGCTTTA AAACTGAGCA GCAAAATAAA 
AAAGCTGCTG AGAAAGAAGA AGAACCCATA CTAGCAGCAA CACAAAAATT GGAAGCAAAT 
GCAGAACCAC TGACAACAAC AAGAATTGAG GTAGCAGTAG CATCTCCACT TGTTGTTAGT 
TCTGCTAGCG TTAAACTCGC TGCTGACGCA ACCAACCAAA TGCGAGCAGC GACGTCGGCA 
GGAGCAGCGA CATTAGCTGA TAAAAATGTA CAAGTTTCGC CCGGTGGAAC GCGACGGTCT 
CGAAGAACAC CCAGACCCAT AGACACGCCC ACTTCAGTTA CTGATGAACA TGTTCAGGTG 
GAGAATAAAA AATTTGGAAA GTCGGAGCAG TATACGGATT GTAGTAGCCA TCTGGAGAGG 
TTCACACTGG ACGATAATAC TGCTATTGTC AGACTGCAGT TGAAAAGTGA ACCGGACAAA 
CCGTCATTAA CTGCATTATC TCCTGAGGAA AACTCGGCTC CTGCTCCGAA GCGAGGAAGG 
GGTCGAGCCA GGAAAATAAG ACCCGATGCC GAGGTGGAAA CCAGTGAGGT TATTTTACCC 
TGCGAGGATT CATTAGGGGA GAAGAAACCA GGACGCAAAA GGAAACTCCC GGATGAACCG 
ATAGATCAGC AGCAACTAAG CGACCTTGTC GTGGTAAAAA CAGAGCAGGA GGAATTGGGT 
GACGCTCCCT TGGGAGATGT TAAGAGAATG CGACGTAGCG TTCGTTTAGG CAATCGATTA 
CACGCCGATG GAAGCCCTTG GGAGGAGGTA AAGACAGAGG CACTTCACCC GCAGCCTTCT 
GCAGAACTTT CCTTTGCGGA GGTCACTTCT GAGATCTTGC CACTAGCTGT CTTAGATGAG 
AAGACCCCAC CCAAAAAGCG AGGGCGGAAG GCAAAAACGC CCTGTGTGAA GTTAGAATCT 
GAAACATCCT GCGGGCTACC GTTTGCTAAT GGCAACAAGA AAACGAATTC CTCTGGTGGA 
TGCGAACTTC AGCTGCCCAA GCGTTCAAAA AGAAGAATAA AACCAACGCC AAAGATATTA 
GAAAATGACG AACTGCGCTG TGAGTTCGAA ACGAAGCACA TTGAGCGTAT GACCCAGTGG 
GAGAGTGCTG CAGCAGTAGA TGGGGATTTT GAAACGCCAA CAACAGGCGG TAATGGGTCC 
AATTCCTCGA CCTCAAGGCA AAAGAGCGAC AAGTCCGACG GGAGCAACTT CGAAGGTGGT 
CCAGGACATC CTGCAGGCAC AAGTGCCATA AAGAAAAGAC TATTTTCAAA GTCCCAGCGG 
GACATTGAGA ACTATGGCGC AGCAATGCTA GCCAAAAGTA AGTTACCTCC GTGTCCGGAT 
GTCGAACAAT TTCTAAACGA CATCAAGGCA TCTCGAATTA ACGCGAATCG GTCACCAGAA 
GAAAGGAAAT TGAACAAAAA ACAGCAACGA AAACTGGCCA AACAAAAGGA AAAGCACCTG 
AAGCATTTGG GCCTGCAAAA AAATCACAGA GATGAGCCCT CGGATAACGA CAGTTCCAAT 
ACGGACAATG AATTCTTTCC TACTACAAGG GTGCAAGTGG GAAAACCAAG TGTTACTTTG 
AGAGTAAGGA ATAGTGTTAC CAAGGAGCTT CCCACCACAG CCACTTTGAA ATCACGCCGG 
AATCCAGTCG TGCAGGCAGC CAAACTCACG AGAAGGATAG GTGCAAGAGC CGCCGGTGAA 
GTTACTGAGG CAGCCCGAGC AAGTGTGCCA ATTTCTACAC CTGATGCGGA GCAGCTTCAC 
TCGTTGGACA CTTCTATTCA AGCTGATGTT ACGCCCATAA GAGACTTAGA TATGAGGCCC 
AGCACCTCCA GAGTGTCAAA GTTTATTTGT CTATGTCAGA AGCCTTCCCA GTATTATGCC 
AGGAATGCCC CGGATTCATC ATACTGCTGT GCCATCGATC ACATCGACGA CCAAAAAATT 
GGCTGCTGCA ACGAGCTGTC CTCAGAGGTT CACAATCTAC TCAGACCCAG CCAACGCGTT 
AGCTATATGA TCCTATGCGA TGAGCACAAA AAGCGCCTAC AATCACACAA CTGCTGCGCA 
GGATGTGGCA TATTTTGTAC ACAGGGAAAA TTTGTGCTAT GCAAGCAGCA GCACTTCTTT 
CATCCCGATT GTGCCCAGCG ATTTATTCTA AGCACTTCGT ACGAGAAGGA ACTCGGCGAT 
GAGGAAGATC AGGGCGTCAA ATTCAGCAGC CCTGTCTTAG TGCTTAAGTG CCCCCACTGC 
GGATTAGATA CACCAGAACG GACCTCCACG GTGACCATGA AGTGTCAGTC CCTGCCTGTC 
TTTCTGCGCA CGCAAAAGTA CAAGATCAAA CCCGCTAGAT TGACAACCTC TTCGCATCTA 
ACCCAATTTG GAACAGTTGA GAATGCCAAT ACCCCAGGAG CCACCGCCAG GAACAAGGGA 
GGACTATCCA CCGCCGTAAC CTTAAGCGCG GCATCAAGTC CTGCCTCAAA AACAAATGGA 
GCCCAGAGAG GCAGGGCGGG CACCTCCAAT TCTAACTCCA GGCATGCTCT TAATTCGATC 
AACTTTGCTC AACTTATACC CGAGTCCGTA ATGAATGTAG TGCTGCGGGG CCACGTGGTT 
TCTGCTAGCG GTCGCGTGAC CGCAGAGTTT ACCCCGCGGG ATATGTACTA TGCAGTCCAA 
AACGATGACT TGGAGCGTGT AGCGGAGATT CTGGCTGCCG ACTTCAACGT GCTGACTCCC 
ATACGGGAGT ACTTAAATGG CACCTGCCTT CATTTGGTGG CCCACTCGGG CACACTTCAG 
ATGGCCTATC TCCTTCTCTG CAAAGGAGCA AGCTCACCGG ATTTCGTTAA TATTGTGGAT 
TATGAACTGC GGACTGCTTT AATGTGTGCC GTGATGAACG AGAAGTGCGA CATGCTGAAT 
CTATTTCTTC AATGTGGTGC GGATGTGGCC ATTAAGGGTC CCGATGGTAA GACCAGTCTG 
CATATTGCTG CCCAGTTAGG CAACTTGGAA GCCACACAGT TAATTGTAGA CAGCTATCGA 
ACTTCTCGAA ATATTACCAG TTTTTTAAGT TTCATTGATG CCCAGGATGA AGGCGGTTGG 
ACGGCCATGG TCTGGGCAGC AGAGCTGGGG CACACGGATA TAGTTAGGTT GGCTTCTCTG 
CCCCAAGCGG TTTTCTTAAA GCTAATTAAT ATTTTTCTCT TCATTAGCTT CCTGCTTAAC 
CAGGATGCTG ATCCAAACAT ATGCGACAAT GACAACAATA CGGTGCTGCA CTGGTCTACG 
CTGCACAATG ATGGTCTGGA CACGATTACA GTACTGCTGC AGTCAGGGGC GGACTGCAAT 
GTACAGAATG TAGAGGGCGA CACGCCTTTG CACATTGCTT GCCGGCATTC TGTCACCCGC 
ATGTGCATCG CGTTAATAGC TAATGGAGCC GATCTAATGA TTAAGAACAA GGCGGAACAA 
CTGCCCTTTG ATTGCATTCC GAATGAGGAG TCTGAGTGCG GTCGCACTGT AGGTTTTAAT 
ATGCAAATGC GTAGTTTCAG GCCTTTGGGC CTCCGTACCT TTGTGGTTTG TGCAGATGCG 
TCGAATGGCA GGGAGGCACG ACCAATCCAG GTGGTTCGCA ACGAACTGGC AATGTCTGAA 
AATGAGGATG AGGCCGATTC GCTTATGTGG CCGGATTTTC GCTACGTAAC ACAATGCATT 
ATCCAGCAGA ATTCAGTGCA GATTGACCGA CGCGTTTCTC AAATGCGCAT CTGCTCCTGC 
CTGGATAGTT GCAGTAGCGA TCGCTGTCAG TGCAATGGAG CCTCCTCGCA GAATTGGTAC 
ACGGCAGAAA GTCGGCTAAA CGCCGACTTT AACTACGAGG ATCCTGCGGT AATCTTCGAA 
TGCAACGACG TCTGCGGTTG CAACCAGCTG TCCTGCAAAA ACCGCGTGGT GCAGAATGGT 
ACACGGACAC CCCTACAAAT TGTCGAGTGC GAGGATCAGG CGAAGGGCTG GGGAGTACGG 
GCGCTGGCCA ATGTACCCAA AGGCACTTTT GTGGGCAGTT ATACGGGTGA GATTTTGACC 
GCGATGGAGG CAGATCGACG CACCGATGAC AGCTACTACT TTGACCTGGA CAACGGGCAC 
TGCATCGATG CCAACTATTA TGGAAATGTA ACCCGGTTTT TTAACCACTC GTGTGAGCCG 
AATGTTTTGC CCGTTCGTGT TTTCTACGAG CATCAGGACT ACAGATTCCC TAAGATCGCC 
TTTTTTTCCT GCCGTGACAT CGATGCCGGA GAGGAAATAT GCTTTGACTA CGGAGAAAAA 
TTCTGGCGCG TGGAACATCG CTCCTGTGTT GGATGTCGAT GCCTGACAAC AACCTGCAAG 
TACGCCTCCC AATCCTCAAG CACAAATGCC TCGCCTACGA ATGCAACAAC GGCACCGGAA 
AATGAAACGG GAACGCTGTC GTCTACAAAT ACGGAGAAAA TTGGACACGC GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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G9a cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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