Pp2A-29B cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pp2A-29B
Entrez Gene ID	2768940
Full Name	Protein phosphatase 2A at 29B
Synonyms	29B,A/PR65,CG13383,CG17291,CG33297,DPR65,Dmel\CG17291,PP2A,PP2A 29B,PP2A A,PP2A-29B,PP2A-A,PP2A[A],PR65,Pp2A,Pp2A29B,dPP2A A,pp2A-29B
General protein information	Preferred Names Protein phosphatase 2A at 29B Names CG17291 gene product from transcript CG17291-RE 65 kDa regulatory subunit of protein phosphatase CG17291-PA CG17291-PB CG17291-PC CG17291-PD CG17291-PE PP2A 65kD subunit A Pp2A-29B-PA Pp2A-29B-PB Pp2A-29B-PC Pp2A-29B-PD Pp2A-29B-PE protein phosphatase 2A protein phosphatase 2A at 29B protein phosphate 2A regulatory subunit A of type 2A protein phosphatase
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 29B2-29B2

mRNA and Protein(s)

mRNA	Protein	Name
NM_001032056.3	NP_001027227.1	protein phosphatase 2A at 29B, isoform A
NM_001032054.3	NP_001027225.1	protein phosphatase 2A at 29B, isoform C
NM_001259015.2	NP_001245944.1	protein phosphatase 2A at 29B, isoform D
NM_001032055.3	NP_001027226.1	protein phosphatase 2A at 29B, isoform B
NM_001298819.1	NP_001285748.1	protein phosphatase 2A at 29B, isoform E

Pathways from BioSystems

Homologies

Eremothecium gossypii	AGOS_ACR279C	NP_983681.1
Neurospora crassa	NCU00488	XP_001728513.1
Bos taurus (cattle)	PPP2R1A	NP_001032554.1
Mus musculus (house mouse)	Ppp2r1a	NP_058587.1
Drosophila melanogaster (fruit fly)	Pp2A-29B	NP_001027225.1
Arabidopsis thaliana (thale cress)	RCN1	NP_173920.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009105	XP_319856.4
Kluyveromyces lactis	KLLA0F07689g	XP_455428.1
Schizosaccharomyces pombe (fission yeast)	paa1	NP_594948.1
Homo sapiens (human)	PPP2R1A	NP_055040.2
Pan troglodytes (chimpanzee)	PPP2R1A	XP_001174546.1
	PPP2R1A	XP_001116604.1
Canis lupus familiaris (dog)	LOC484335	XP_850993.1
Rattus norvegicus (Norway rat)	Ppp2r1a	NP_476481.1
Caenorhabditis elegans (roundworm)	paa-1	NP_498162.2
Magnaporthe oryzae (rice blast fungus)	MGG_05671	XP_003710570.1
Xenopus tropicalis (tropical clawed frog)	ppp2r1a	NP_989405.1
Saccharomyces cerevisiae (baker's yeast)	TPD3	NP_009386.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

This study show that protein phosphatase 2A (PP2A) is required for the development of structurally normal active zones opposite glutamate receptors. When PP2A is inhibited presynaptically.
Title: PP2A and GSK-3beta act antagonistically to regulate active zone development.

The balance of PAR-1 and PP2A effects on Baz phosphorylation determines neuroblasts polarity.
Title: PP2A antagonizes phosphorylation of Bazooka by PAR-1 to control apical-basal polarity in dividing embryonic neuroblasts.

B56epsilon is alternatively translated adds a new level of regulation to PP2A holoenzymes
Title: The 48-kDa alternative translation isoform of PP2A:B56epsilon is required for Wnt signaling during midbrain-hindbrain boundary formation.

Dephosphorylation of inactivation no afterpotential D (INAD) protein is catalyzed by PP2A, thereby modulating the fast deactivation of the visual response.
Title: Role of protein phosphatase 2A in regulating the visual signaling in Drosophila.

The following Pp2A-29B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pp2A-29B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa15536	NM_001032056.3 Latest version!	Drosophila melanogaster protein phosphatase 2A at 29B, transcript variant A (Pp2A-29B), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa15536	NM_001032055.3 Latest version!	Drosophila melanogaster protein phosphatase 2A at 29B, transcript variant B (Pp2A-29B), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa15536	NM_001032054.3 Latest version!	Drosophila melanogaster protein phosphatase 2A at 29B, transcript variant C (Pp2A-29B), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa16562	NM_001259015.2 Latest version!	Drosophila melanogaster protein phosphatase 2A at 29B, transcript variant D (Pp2A-29B), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa22360	NM_001298819.1 Latest version!	Drosophila melanogaster protein phosphatase 2A at 29B, transcript variant E (Pp2A-29B), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pp2A-29B ( NM_001032056.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001027227.1 Drosophila melanogaster protein phosphatase 2A at 29B, transcript variant A (Pp2A-29B), mRNA.

Price & Availability↑

CloneID	OFa15536
Clone ID Related Accession (Same CDS sequence)	NM_001032056.3 , NM_001032054.3 , NM_001032055.3
Accession Version	NM_001032056.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1776bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	protein phosphatase 2A at 29B, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_001032056.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCAGCAA GCGACAAATC GGTCGACGAT TCACTATATC CCATTGCGGT TCTAATCGAT 
GAACTGAAAA ACGAGGACGT TCAGCTTCGG TTGAACTCCA TCAAGAAACT GTCCACCATT 
GCACTCGCTT TGGGCGAGGA GCGCACACGG TCCGAGTTGA TTCCCTTCCT CACCGAGACC 
ATATACGATG AGGACGAGGT ACTGCTGGCC CTGGCCGACC AACTGGGCAA CTTTACTAGT 
CTCGTTGGTG GGCCAGAGTT TGCCATGTAC TTGATTCCGC CCCTCGAGAG TTTGGCCACC 
GTAGAGGAAA CCGTGGTGCG AGACAAGGCT GTGGAATCTC TACGCACCGT GGCCGCTGAG 
CACAGCGCCC AGGATTTGGA GATCCATGTG GTGCCGACAC TGCAGCGATT GGTTTCCGGT 
GACTGGTTCA CCTCACGCAC CTCTGCCTGC GGCCTCTTCT CGGTCTGCTA TCCACGCGTC 
ACACAGCCAG TGAAGGCCGA GCTGCGCGCC AACTTCCGAA AGCTCTGCCA GGATGAGACA 
CCCATGGTGC GCCGTGCAGC GGCCAACAAG CTGGGCGAGT TTGCCAAGGT CGTTGAGACG 
GAGTATCTGA AGTCCGATTT GATTCCCAAC TTTGTCCAGC TGGCACAGGA TGATCAGGAC 
TCTGTCCGTC TGCTGGCTGT AGAGGCATGC GTAAGCATTG CCCAGCTGCT GCCTCAGGAT 
GATGTAGAGC ACCTGGTTCT GCCCACGCTG CGCCAGTGCG CCAGCGACTC TTCCTGGAGG 
GTGCGTTACA TGGTGGCCGA GAAGTTTGTT GATCTGCAAA AGGCTGTGGG CCCAGAGATT 
ACTAGGGTGG ACTTGGTGCC TGCCTTCCAG TACTTGCTCA AGGATGCCGA GGCCGAGGTT 
CGCGCTGCAG TGGCCACCAA GGTGAAGGAC TTCTGCGCCA ATCTGGACAA GGTCAACCAG 
GTGCAAATCA TCCTTAGTTC CATTTTGCCC TATGTCCGCG ATCTTGTCTC GGACCCCAAT 
CCTCATGTGA AGTCAGCTCT GGCCTCAGTG ATCATGGGCT TGAGTCCCAT GCTGGGCGCC 
TATCAGACTG TGGAGCAATT GCTCCCCCTG TTCCTTATTC AACTCAAGGA TGAGTGCCCA 
GAAGTGCGCC TAAACATCAT CTCAAACCTG GATTGCGTTA ACGACGTCAT CGGTATCCAG 
CAACTGTCAC AGTCGCTTCT GCCCGCCATC GTCGAGCTGG CCGAGGACTC CAAGTGGCGT 
GTGCGTCTAG CCATCATCGA GTACATGCCT GCTCTGGCCG GTCAGTTGGG TCAGGAATTC 
TTTGACCAAA AACTGCGCGG TCTCTGCATG GGATGGCTCA ACGATCACGT GTACGCCATT 
CGTGAGGCAG CCACCCTCAA CATGAAGAAG CTCGTCGAGC AGTTCGGAGC TCCCTGGGCC 
GAACAGGCCA TAATTCCAAT GATTCTGGTT ATGTCGCGCA ACAAGAACTA TTTGCACAGA 
ATGACTTGCT TGTTCTGCCT GAATGTTTTG GCAGAGGTCT GCGGCACAGA TATCACCACC 
AAGTTGCTGC TGCCCACAGT TCTCCTGCTT GCCGCTGATC CCGTTGCCAA TGTTCGTTTC 
AACGTGGCAA AGACCCTGCA GAAGATCTCG CCCTTCCTGG AGGCCAGCGT CATTGATGCC 
CAAGTAAAGC CCACACTCGA CAAACTGAAC ACAGACACAG ATGTGGATGT CAAGCATTTT 
GCTGCACAGG CCATTGCCGG CATAGCTGCA GCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001027227.1
CDS	274..2049
Translation	MAASDKSVDDSLYPIAVLIDELKNEDVQLRLNSIKKLSTIALALGEERTRSELIPFLTETIYDEDEVLLALADQLGNFTSLVGGPEFAMYLIPPLESLATVEETVVRDKAVESLRTVAAEHSAQDLEIHVVPTLQRLVSGDWFTSRTSACGLFSVCYPRVTQPVKAELRANFRKLCQDETPMVRRAAANKLGEFAKVVETEYLKSDLIPNFVQLAQDDQDSVRLLAVEACVSIAQLLPQDDVEHLVLPTLRQCASDSSWRVRYMVAEKFVDLQKAVGPEITRVDLVPAFQYLLKDAEAEVRAAVATKVKDFCANLDKVNQVQIILSSILPYVRDLVSDPNPHVKSALASVIMGLSPMLGAYQTVEQLLPLFLIQLKDECPEVRLNIISNLDCVNDVIGIQQLSQSLLPAIVELAEDSKWRVRLAIIEYMPALAGQLGQEFFDQKLRGLCMGWLNDHVYAIREAATLNMKKLVEQFGAPWAEQAIIPMILVMSRNKNYLHRMTCLFCLNVLAEVCGTDITTKLLLPTVLLLAADPVANVRFNVAKTLQKISPFLEASVIDAQVKPTLDKLNTDTDVDVKHFAAQAIAGIAAA

Target ORF information:

RefSeq Version	NM_001032056.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster protein phosphatase 2A at 29B, transcript variant A (Pp2A-29B), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001032056.3

ORF Insert Sequence:

ATGGCAGCAA GCGACAAATC GGTCGACGAT TCACTATATC CCATTGCGGT TCTAATCGAT 
GAACTGAAAA ACGAGGACGT TCAGCTTCGG TTGAACTCCA TCAAGAAACT GTCCACCATT 
GCACTCGCTT TGGGCGAGGA GCGCACACGG TCCGAGTTGA TTCCCTTCCT CACCGAGACC 
ATATACGATG AGGACGAGGT ACTGCTGGCC CTGGCCGACC AACTGGGCAA CTTTACTAGT 
CTCGTTGGTG GGCCAGAGTT TGCCATGTAC TTGATTCCGC CCCTCGAGAG TTTGGCCACC 
GTAGAGGAAA CCGTGGTGCG AGACAAGGCT GTGGAATCTC TACGCACCGT GGCCGCTGAG 
CACAGCGCCC AGGATTTGGA GATCCATGTG GTGCCGACAC TGCAGCGATT GGTTTCCGGT 
GACTGGTTCA CCTCACGCAC CTCTGCCTGC GGCCTCTTCT CGGTCTGCTA TCCACGCGTC 
ACACAGCCAG TGAAGGCCGA GCTGCGCGCC AACTTCCGAA AGCTCTGCCA GGATGAGACA 
CCCATGGTGC GCCGTGCAGC GGCCAACAAG CTGGGCGAGT TTGCCAAGGT CGTTGAGACG 
GAGTATCTGA AGTCCGATTT GATTCCCAAC TTTGTCCAGC TGGCACAGGA TGATCAGGAC 
TCTGTCCGTC TGCTGGCTGT AGAGGCATGC GTAAGCATTG CCCAGCTGCT GCCTCAGGAT 
GATGTAGAGC ACCTGGTTCT GCCCACGCTG CGCCAGTGCG CCAGCGACTC TTCCTGGAGG 
GTGCGTTACA TGGTGGCCGA GAAGTTTGTT GATCTGCAAA AGGCTGTGGG CCCAGAGATT 
ACTAGGGTGG ACTTGGTGCC TGCCTTCCAG TACTTGCTCA AGGATGCCGA GGCCGAGGTT 
CGCGCTGCAG TGGCCACCAA GGTGAAGGAC TTCTGCGCCA ATCTGGACAA GGTCAACCAG 
GTGCAAATCA TCCTTAGTTC CATTTTGCCC TATGTCCGCG ATCTTGTCTC GGACCCCAAT 
CCTCATGTGA AGTCAGCTCT GGCCTCAGTG ATCATGGGCT TGAGTCCCAT GCTGGGCGCC 
TATCAGACTG TGGAGCAATT GCTCCCCCTG TTCCTTATTC AACTCAAGGA TGAGTGCCCA 
GAAGTGCGCC TAAACATCAT CTCAAACCTG GATTGCGTTA ACGACGTCAT CGGTATCCAG 
CAACTGTCAC AGTCGCTTCT GCCCGCCATC GTCGAGCTGG CCGAGGACTC CAAGTGGCGT 
GTGCGTCTAG CCATCATCGA GTACATGCCT GCTCTGGCCG GTCAGTTGGG TCAGGAATTC 
TTTGACCAAA AACTGCGCGG TCTCTGCATG GGATGGCTCA ACGATCACGT GTACGCCATT 
CGTGAGGCAG CCACCCTCAA CATGAAGAAG CTCGTCGAGC AGTTCGGAGC TCCCTGGGCC 
GAACAGGCCA TAATTCCAAT GATTCTGGTT ATGTCGCGCA ACAAGAACTA TTTGCACAGA 
ATGACTTGCT TGTTCTGCCT GAATGTTTTG GCAGAGGTCT GCGGCACAGA TATCACCACC 
AAGTTGCTGC TGCCCACAGT TCTCCTGCTT GCCGCTGATC CCGTTGCCAA TGTTCGTTTC 
AACGTGGCAA AGACCCTGCA GAAGATCTCG CCCTTCCTGG AGGCCAGCGT CATTGATGCC 
CAAGTAAAGC CCACACTCGA CAAACTGAAC ACAGACACAG ATGTGGATGT CAAGCATTTT 
GCTGCACAGG CCATTGCCGG CATAGCTGCA GCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pp2A-29B ( NM_001032054.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001027225.1 Drosophila melanogaster protein phosphatase 2A at 29B, transcript variant C (Pp2A-29B), mRNA.

Price & Availability↑

CloneID	OFa15536
Clone ID Related Accession (Same CDS sequence)	NM_001032056.3 , NM_001032054.3 , NM_001032055.3
Accession Version	NM_001032054.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1776bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	protein phosphatase 2A at 29B, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_001032054.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCAGCAA GCGACAAATC GGTCGACGAT TCACTATATC CCATTGCGGT TCTAATCGAT 
GAACTGAAAA ACGAGGACGT TCAGCTTCGG TTGAACTCCA TCAAGAAACT GTCCACCATT 
GCACTCGCTT TGGGCGAGGA GCGCACACGG TCCGAGTTGA TTCCCTTCCT CACCGAGACC 
ATATACGATG AGGACGAGGT ACTGCTGGCC CTGGCCGACC AACTGGGCAA CTTTACTAGT 
CTCGTTGGTG GGCCAGAGTT TGCCATGTAC TTGATTCCGC CCCTCGAGAG TTTGGCCACC 
GTAGAGGAAA CCGTGGTGCG AGACAAGGCT GTGGAATCTC TACGCACCGT GGCCGCTGAG 
CACAGCGCCC AGGATTTGGA GATCCATGTG GTGCCGACAC TGCAGCGATT GGTTTCCGGT 
GACTGGTTCA CCTCACGCAC CTCTGCCTGC GGCCTCTTCT CGGTCTGCTA TCCACGCGTC 
ACACAGCCAG TGAAGGCCGA GCTGCGCGCC AACTTCCGAA AGCTCTGCCA GGATGAGACA 
CCCATGGTGC GCCGTGCAGC GGCCAACAAG CTGGGCGAGT TTGCCAAGGT CGTTGAGACG 
GAGTATCTGA AGTCCGATTT GATTCCCAAC TTTGTCCAGC TGGCACAGGA TGATCAGGAC 
TCTGTCCGTC TGCTGGCTGT AGAGGCATGC GTAAGCATTG CCCAGCTGCT GCCTCAGGAT 
GATGTAGAGC ACCTGGTTCT GCCCACGCTG CGCCAGTGCG CCAGCGACTC TTCCTGGAGG 
GTGCGTTACA TGGTGGCCGA GAAGTTTGTT GATCTGCAAA AGGCTGTGGG CCCAGAGATT 
ACTAGGGTGG ACTTGGTGCC TGCCTTCCAG TACTTGCTCA AGGATGCCGA GGCCGAGGTT 
CGCGCTGCAG TGGCCACCAA GGTGAAGGAC TTCTGCGCCA ATCTGGACAA GGTCAACCAG 
GTGCAAATCA TCCTTAGTTC CATTTTGCCC TATGTCCGCG ATCTTGTCTC GGACCCCAAT 
CCTCATGTGA AGTCAGCTCT GGCCTCAGTG ATCATGGGCT TGAGTCCCAT GCTGGGCGCC 
TATCAGACTG TGGAGCAATT GCTCCCCCTG TTCCTTATTC AACTCAAGGA TGAGTGCCCA 
GAAGTGCGCC TAAACATCAT CTCAAACCTG GATTGCGTTA ACGACGTCAT CGGTATCCAG 
CAACTGTCAC AGTCGCTTCT GCCCGCCATC GTCGAGCTGG CCGAGGACTC CAAGTGGCGT 
GTGCGTCTAG CCATCATCGA GTACATGCCT GCTCTGGCCG GTCAGTTGGG TCAGGAATTC 
TTTGACCAAA AACTGCGCGG TCTCTGCATG GGATGGCTCA ACGATCACGT GTACGCCATT 
CGTGAGGCAG CCACCCTCAA CATGAAGAAG CTCGTCGAGC AGTTCGGAGC TCCCTGGGCC 
GAACAGGCCA TAATTCCAAT GATTCTGGTT ATGTCGCGCA ACAAGAACTA TTTGCACAGA 
ATGACTTGCT TGTTCTGCCT GAATGTTTTG GCAGAGGTCT GCGGCACAGA TATCACCACC 
AAGTTGCTGC TGCCCACAGT TCTCCTGCTT GCCGCTGATC CCGTTGCCAA TGTTCGTTTC 
AACGTGGCAA AGACCCTGCA GAAGATCTCG CCCTTCCTGG AGGCCAGCGT CATTGATGCC 
CAAGTAAAGC CCACACTCGA CAAACTGAAC ACAGACACAG ATGTGGATGT CAAGCATTTT 
GCTGCACAGG CCATTGCCGG CATAGCTGCA GCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001027225.1
CDS	218..1993
Translation	MAASDKSVDDSLYPIAVLIDELKNEDVQLRLNSIKKLSTIALALGEERTRSELIPFLTETIYDEDEVLLALADQLGNFTSLVGGPEFAMYLIPPLESLATVEETVVRDKAVESLRTVAAEHSAQDLEIHVVPTLQRLVSGDWFTSRTSACGLFSVCYPRVTQPVKAELRANFRKLCQDETPMVRRAAANKLGEFAKVVETEYLKSDLIPNFVQLAQDDQDSVRLLAVEACVSIAQLLPQDDVEHLVLPTLRQCASDSSWRVRYMVAEKFVDLQKAVGPEITRVDLVPAFQYLLKDAEAEVRAAVATKVKDFCANLDKVNQVQIILSSILPYVRDLVSDPNPHVKSALASVIMGLSPMLGAYQTVEQLLPLFLIQLKDECPEVRLNIISNLDCVNDVIGIQQLSQSLLPAIVELAEDSKWRVRLAIIEYMPALAGQLGQEFFDQKLRGLCMGWLNDHVYAIREAATLNMKKLVEQFGAPWAEQAIIPMILVMSRNKNYLHRMTCLFCLNVLAEVCGTDITTKLLLPTVLLLAADPVANVRFNVAKTLQKISPFLEASVIDAQVKPTLDKLNTDTDVDVKHFAAQAIAGIAAA

Target ORF information:

RefSeq Version	NM_001032054.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster protein phosphatase 2A at 29B, transcript variant C (Pp2A-29B), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001032054.3

ORF Insert Sequence:

ATGGCAGCAA GCGACAAATC GGTCGACGAT TCACTATATC CCATTGCGGT TCTAATCGAT 
GAACTGAAAA ACGAGGACGT TCAGCTTCGG TTGAACTCCA TCAAGAAACT GTCCACCATT 
GCACTCGCTT TGGGCGAGGA GCGCACACGG TCCGAGTTGA TTCCCTTCCT CACCGAGACC 
ATATACGATG AGGACGAGGT ACTGCTGGCC CTGGCCGACC AACTGGGCAA CTTTACTAGT 
CTCGTTGGTG GGCCAGAGTT TGCCATGTAC TTGATTCCGC CCCTCGAGAG TTTGGCCACC 
GTAGAGGAAA CCGTGGTGCG AGACAAGGCT GTGGAATCTC TACGCACCGT GGCCGCTGAG 
CACAGCGCCC AGGATTTGGA GATCCATGTG GTGCCGACAC TGCAGCGATT GGTTTCCGGT 
GACTGGTTCA CCTCACGCAC CTCTGCCTGC GGCCTCTTCT CGGTCTGCTA TCCACGCGTC 
ACACAGCCAG TGAAGGCCGA GCTGCGCGCC AACTTCCGAA AGCTCTGCCA GGATGAGACA 
CCCATGGTGC GCCGTGCAGC GGCCAACAAG CTGGGCGAGT TTGCCAAGGT CGTTGAGACG 
GAGTATCTGA AGTCCGATTT GATTCCCAAC TTTGTCCAGC TGGCACAGGA TGATCAGGAC 
TCTGTCCGTC TGCTGGCTGT AGAGGCATGC GTAAGCATTG CCCAGCTGCT GCCTCAGGAT 
GATGTAGAGC ACCTGGTTCT GCCCACGCTG CGCCAGTGCG CCAGCGACTC TTCCTGGAGG 
GTGCGTTACA TGGTGGCCGA GAAGTTTGTT GATCTGCAAA AGGCTGTGGG CCCAGAGATT 
ACTAGGGTGG ACTTGGTGCC TGCCTTCCAG TACTTGCTCA AGGATGCCGA GGCCGAGGTT 
CGCGCTGCAG TGGCCACCAA GGTGAAGGAC TTCTGCGCCA ATCTGGACAA GGTCAACCAG 
GTGCAAATCA TCCTTAGTTC CATTTTGCCC TATGTCCGCG ATCTTGTCTC GGACCCCAAT 
CCTCATGTGA AGTCAGCTCT GGCCTCAGTG ATCATGGGCT TGAGTCCCAT GCTGGGCGCC 
TATCAGACTG TGGAGCAATT GCTCCCCCTG TTCCTTATTC AACTCAAGGA TGAGTGCCCA 
GAAGTGCGCC TAAACATCAT CTCAAACCTG GATTGCGTTA ACGACGTCAT CGGTATCCAG 
CAACTGTCAC AGTCGCTTCT GCCCGCCATC GTCGAGCTGG CCGAGGACTC CAAGTGGCGT 
GTGCGTCTAG CCATCATCGA GTACATGCCT GCTCTGGCCG GTCAGTTGGG TCAGGAATTC 
TTTGACCAAA AACTGCGCGG TCTCTGCATG GGATGGCTCA ACGATCACGT GTACGCCATT 
CGTGAGGCAG CCACCCTCAA CATGAAGAAG CTCGTCGAGC AGTTCGGAGC TCCCTGGGCC 
GAACAGGCCA TAATTCCAAT GATTCTGGTT ATGTCGCGCA ACAAGAACTA TTTGCACAGA 
ATGACTTGCT TGTTCTGCCT GAATGTTTTG GCAGAGGTCT GCGGCACAGA TATCACCACC 
AAGTTGCTGC TGCCCACAGT TCTCCTGCTT GCCGCTGATC CCGTTGCCAA TGTTCGTTTC 
AACGTGGCAA AGACCCTGCA GAAGATCTCG CCCTTCCTGG AGGCCAGCGT CATTGATGCC 
CAAGTAAAGC CCACACTCGA CAAACTGAAC ACAGACACAG ATGTGGATGT CAAGCATTTT 
GCTGCACAGG CCATTGCCGG CATAGCTGCA GCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pp2A-29B ( NM_001032055.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001027226.1 Drosophila melanogaster protein phosphatase 2A at 29B, transcript variant B (Pp2A-29B), mRNA.

Price & Availability↑

CloneID	OFa15536
Clone ID Related Accession (Same CDS sequence)	NM_001032056.3 , NM_001032054.3 , NM_001032055.3
Accession Version	NM_001032055.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1776bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	protein phosphatase 2A at 29B, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_001032055.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCAGCAA GCGACAAATC GGTCGACGAT TCACTATATC CCATTGCGGT TCTAATCGAT 
GAACTGAAAA ACGAGGACGT TCAGCTTCGG TTGAACTCCA TCAAGAAACT GTCCACCATT 
GCACTCGCTT TGGGCGAGGA GCGCACACGG TCCGAGTTGA TTCCCTTCCT CACCGAGACC 
ATATACGATG AGGACGAGGT ACTGCTGGCC CTGGCCGACC AACTGGGCAA CTTTACTAGT 
CTCGTTGGTG GGCCAGAGTT TGCCATGTAC TTGATTCCGC CCCTCGAGAG TTTGGCCACC 
GTAGAGGAAA CCGTGGTGCG AGACAAGGCT GTGGAATCTC TACGCACCGT GGCCGCTGAG 
CACAGCGCCC AGGATTTGGA GATCCATGTG GTGCCGACAC TGCAGCGATT GGTTTCCGGT 
GACTGGTTCA CCTCACGCAC CTCTGCCTGC GGCCTCTTCT CGGTCTGCTA TCCACGCGTC 
ACACAGCCAG TGAAGGCCGA GCTGCGCGCC AACTTCCGAA AGCTCTGCCA GGATGAGACA 
CCCATGGTGC GCCGTGCAGC GGCCAACAAG CTGGGCGAGT TTGCCAAGGT CGTTGAGACG 
GAGTATCTGA AGTCCGATTT GATTCCCAAC TTTGTCCAGC TGGCACAGGA TGATCAGGAC 
TCTGTCCGTC TGCTGGCTGT AGAGGCATGC GTAAGCATTG CCCAGCTGCT GCCTCAGGAT 
GATGTAGAGC ACCTGGTTCT GCCCACGCTG CGCCAGTGCG CCAGCGACTC TTCCTGGAGG 
GTGCGTTACA TGGTGGCCGA GAAGTTTGTT GATCTGCAAA AGGCTGTGGG CCCAGAGATT 
ACTAGGGTGG ACTTGGTGCC TGCCTTCCAG TACTTGCTCA AGGATGCCGA GGCCGAGGTT 
CGCGCTGCAG TGGCCACCAA GGTGAAGGAC TTCTGCGCCA ATCTGGACAA GGTCAACCAG 
GTGCAAATCA TCCTTAGTTC CATTTTGCCC TATGTCCGCG ATCTTGTCTC GGACCCCAAT 
CCTCATGTGA AGTCAGCTCT GGCCTCAGTG ATCATGGGCT TGAGTCCCAT GCTGGGCGCC 
TATCAGACTG TGGAGCAATT GCTCCCCCTG TTCCTTATTC AACTCAAGGA TGAGTGCCCA 
GAAGTGCGCC TAAACATCAT CTCAAACCTG GATTGCGTTA ACGACGTCAT CGGTATCCAG 
CAACTGTCAC AGTCGCTTCT GCCCGCCATC GTCGAGCTGG CCGAGGACTC CAAGTGGCGT 
GTGCGTCTAG CCATCATCGA GTACATGCCT GCTCTGGCCG GTCAGTTGGG TCAGGAATTC 
TTTGACCAAA AACTGCGCGG TCTCTGCATG GGATGGCTCA ACGATCACGT GTACGCCATT 
CGTGAGGCAG CCACCCTCAA CATGAAGAAG CTCGTCGAGC AGTTCGGAGC TCCCTGGGCC 
GAACAGGCCA TAATTCCAAT GATTCTGGTT ATGTCGCGCA ACAAGAACTA TTTGCACAGA 
ATGACTTGCT TGTTCTGCCT GAATGTTTTG GCAGAGGTCT GCGGCACAGA TATCACCACC 
AAGTTGCTGC TGCCCACAGT TCTCCTGCTT GCCGCTGATC CCGTTGCCAA TGTTCGTTTC 
AACGTGGCAA AGACCCTGCA GAAGATCTCG CCCTTCCTGG AGGCCAGCGT CATTGATGCC 
CAAGTAAAGC CCACACTCGA CAAACTGAAC ACAGACACAG ATGTGGATGT CAAGCATTTT 
GCTGCACAGG CCATTGCCGG CATAGCTGCA GCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001027226.1
CDS	205..1980
Translation	MAASDKSVDDSLYPIAVLIDELKNEDVQLRLNSIKKLSTIALALGEERTRSELIPFLTETIYDEDEVLLALADQLGNFTSLVGGPEFAMYLIPPLESLATVEETVVRDKAVESLRTVAAEHSAQDLEIHVVPTLQRLVSGDWFTSRTSACGLFSVCYPRVTQPVKAELRANFRKLCQDETPMVRRAAANKLGEFAKVVETEYLKSDLIPNFVQLAQDDQDSVRLLAVEACVSIAQLLPQDDVEHLVLPTLRQCASDSSWRVRYMVAEKFVDLQKAVGPEITRVDLVPAFQYLLKDAEAEVRAAVATKVKDFCANLDKVNQVQIILSSILPYVRDLVSDPNPHVKSALASVIMGLSPMLGAYQTVEQLLPLFLIQLKDECPEVRLNIISNLDCVNDVIGIQQLSQSLLPAIVELAEDSKWRVRLAIIEYMPALAGQLGQEFFDQKLRGLCMGWLNDHVYAIREAATLNMKKLVEQFGAPWAEQAIIPMILVMSRNKNYLHRMTCLFCLNVLAEVCGTDITTKLLLPTVLLLAADPVANVRFNVAKTLQKISPFLEASVIDAQVKPTLDKLNTDTDVDVKHFAAQAIAGIAAA

Target ORF information:

RefSeq Version	NM_001032055.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster protein phosphatase 2A at 29B, transcript variant B (Pp2A-29B), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001032055.3

ORF Insert Sequence:

ATGGCAGCAA GCGACAAATC GGTCGACGAT TCACTATATC CCATTGCGGT TCTAATCGAT 
GAACTGAAAA ACGAGGACGT TCAGCTTCGG TTGAACTCCA TCAAGAAACT GTCCACCATT 
GCACTCGCTT TGGGCGAGGA GCGCACACGG TCCGAGTTGA TTCCCTTCCT CACCGAGACC 
ATATACGATG AGGACGAGGT ACTGCTGGCC CTGGCCGACC AACTGGGCAA CTTTACTAGT 
CTCGTTGGTG GGCCAGAGTT TGCCATGTAC TTGATTCCGC CCCTCGAGAG TTTGGCCACC 
GTAGAGGAAA CCGTGGTGCG AGACAAGGCT GTGGAATCTC TACGCACCGT GGCCGCTGAG 
CACAGCGCCC AGGATTTGGA GATCCATGTG GTGCCGACAC TGCAGCGATT GGTTTCCGGT 
GACTGGTTCA CCTCACGCAC CTCTGCCTGC GGCCTCTTCT CGGTCTGCTA TCCACGCGTC 
ACACAGCCAG TGAAGGCCGA GCTGCGCGCC AACTTCCGAA AGCTCTGCCA GGATGAGACA 
CCCATGGTGC GCCGTGCAGC GGCCAACAAG CTGGGCGAGT TTGCCAAGGT CGTTGAGACG 
GAGTATCTGA AGTCCGATTT GATTCCCAAC TTTGTCCAGC TGGCACAGGA TGATCAGGAC 
TCTGTCCGTC TGCTGGCTGT AGAGGCATGC GTAAGCATTG CCCAGCTGCT GCCTCAGGAT 
GATGTAGAGC ACCTGGTTCT GCCCACGCTG CGCCAGTGCG CCAGCGACTC TTCCTGGAGG 
GTGCGTTACA TGGTGGCCGA GAAGTTTGTT GATCTGCAAA AGGCTGTGGG CCCAGAGATT 
ACTAGGGTGG ACTTGGTGCC TGCCTTCCAG TACTTGCTCA AGGATGCCGA GGCCGAGGTT 
CGCGCTGCAG TGGCCACCAA GGTGAAGGAC TTCTGCGCCA ATCTGGACAA GGTCAACCAG 
GTGCAAATCA TCCTTAGTTC CATTTTGCCC TATGTCCGCG ATCTTGTCTC GGACCCCAAT 
CCTCATGTGA AGTCAGCTCT GGCCTCAGTG ATCATGGGCT TGAGTCCCAT GCTGGGCGCC 
TATCAGACTG TGGAGCAATT GCTCCCCCTG TTCCTTATTC AACTCAAGGA TGAGTGCCCA 
GAAGTGCGCC TAAACATCAT CTCAAACCTG GATTGCGTTA ACGACGTCAT CGGTATCCAG 
CAACTGTCAC AGTCGCTTCT GCCCGCCATC GTCGAGCTGG CCGAGGACTC CAAGTGGCGT 
GTGCGTCTAG CCATCATCGA GTACATGCCT GCTCTGGCCG GTCAGTTGGG TCAGGAATTC 
TTTGACCAAA AACTGCGCGG TCTCTGCATG GGATGGCTCA ACGATCACGT GTACGCCATT 
CGTGAGGCAG CCACCCTCAA CATGAAGAAG CTCGTCGAGC AGTTCGGAGC TCCCTGGGCC 
GAACAGGCCA TAATTCCAAT GATTCTGGTT ATGTCGCGCA ACAAGAACTA TTTGCACAGA 
ATGACTTGCT TGTTCTGCCT GAATGTTTTG GCAGAGGTCT GCGGCACAGA TATCACCACC 
AAGTTGCTGC TGCCCACAGT TCTCCTGCTT GCCGCTGATC CCGTTGCCAA TGTTCGTTTC 
AACGTGGCAA AGACCCTGCA GAAGATCTCG CCCTTCCTGG AGGCCAGCGT CATTGATGCC 
CAAGTAAAGC CCACACTCGA CAAACTGAAC ACAGACACAG ATGTGGATGT CAAGCATTTT 
GCTGCACAGG CCATTGCCGG CATAGCTGCA GCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pp2A-29B ( NM_001259015.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001245944.1 Drosophila melanogaster protein phosphatase 2A at 29B, transcript variant D (Pp2A-29B), mRNA.

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CloneID	OFa16562
Clone ID Related Accession (Same CDS sequence)	NM_001259015.2
Accession Version	NM_001259015.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1953bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	protein phosphatase 2A at 29B, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_001259015.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCAGCAA GCGACAAATC GGTCGACGAT TCACTATATC CCATTGCGGT TCTAATCGAT 
GAACTGAAAA ACGAGGACGT TCAGCTTCGG TTGAACTCCA TCAAGAAACT GTCCACCATT 
GCACTCGCTT TGGGCGAGGA GCGCACACGG TCCGAGTTGA TTCCCTTCCT CACCGAGACC 
ATATACGATG AGGACGAGGT ACTGCTGGCC CTGGCCGACC AACTGGGCAA CTTTACTAGT 
CTCGTTGGTG GGCCAGAGTT TGCCATGTAC TTGATTCCGC CCCTCGAGAG TTTGGCCACC 
GTAGAGGAAA CCGTGGTGCG AGACAAGGCT GTGGAATCTC TACGCACCGT GGCCGCTGAG 
CACAGCGCCC AGGATTTGGA GATCCATGTG GTGCCGACAC TGCAGCGATT GGTTTCCGGT 
GACTGGTTCA CCTCACGCAC CTCTGCCTGC GGCCTCTTCT CGGTCTGCTA TCCACGCGTC 
ACACAGCCAG TGAAGGCCGA GCTGCGCGCC AACTTCCGAA AGCTCTGCCA GGATGAGACA 
CCCATGGTGC GCCGTGCAGC GGCCAACAAG CTGGGCGAGT TTGCCAAGGT CGTTGAGACG 
GAGTATCTGA AGTCCGATTT GATTCCCAAC TTTGTCCAGC TGGCACAGGA TGATCAGGAC 
TCTGTCCGTC TGCTGGCTGT AGAGGCATGC GTAAGCATTG CCCAGCTGCT GCCTCAGGAT 
GATGTAGAGC ACCTGGTTCT GCCCACGCTG CGCCAGTGCG CCAGCGACTC TTCCTGGAGG 
GTGCGTTACA TGGTGGCCGA GAAGTTTGTT GATCTGCAAA AGGCTGTGGG CCCAGAGATT 
ACTAGGGTGG ACTTGGTGCC TGCCTTCCAG TACTTGCTCA AGGATGCCGA GGCCGAGGTT 
CGCGCTGCAG TGGCCACCAA GGTGAAGGAC TTCTGCGCCA ATCTGGACAA GGTCAACCAG 
GTGCAAATCA TCCTTAGTTC CATTTTGCCC TATGTCCGCG ATCTTGTCTC GGACCCCAAT 
CCTCATGTGA AGTCAGCTCT GGCCTCAGTG ATCATGGGCT TGAGTCCCAT GCTGGGCGCC 
TATCAGACTG TGGAGCAATT GCTCCCCCTG TTCCTTATTC AACTCAAGGA TGAGTGCCCA 
GAAGTGCGCC TAAACATCAT CTCAAACCTG GATTGCGTTA ACGACGTCAT CGGTATCCAG 
CAACTGTCAC AGTCGCTTCT GCCCGCCATC GTCGAGCTGG CCGAGGACTC CAAGTGGCGT 
GTGCGTCTAG CCATCATCGA GTACATGCCT GCTCTGGCCG GTCAGTTGGG TCAGGAATTC 
TTTGACCAAA AACTGCGCGG TCTCTGCATG GGATGGCTCA ACGATCACGT GTACGCCATT 
CGTGAGGCAG CCACCCTCAA CATGAAGAAG CTCGTCGAGC AGTTCGGAGC TCCCTGGGCC 
GAACAGGCCA TAATTCCAAT GATTCTGGTT ATGTCGCGCA ACAAGAACTA TTTGCACAGA 
ATGACTTGCT TGTTCTGCCT GAATGTTTTG GCAGAGGTCT GCGGCACAGA TATCACCACC 
AAGTTGCTGC TGCCCACAGT TCTCCTGCTT GCCGCTGATC CCGTTGCCAA TGTTCGTTTC 
AACGTGGCAA AGACCCTGCA GAAGATCTCG CCCTTCCTGG AGGCCAGCGT CATTGATGCC 
CAAGTAAAGC CCACACTCGA CAAACTGAAC ACAGACACAG ATGTGGATGT CAAGCATTTT 
GCTGCACAGG CCATTGCCGG CATAGCTGCA GAAATGGAAC TGAATGTGTT TAGAACGGCA 
ATACCGCCGT GCATGGGCGC CAAAATTGAG GCGGCAATGG ACTCAAAGCT GATAGTGGAA 
CCGCCCCGAC AGCCGGAGGC AGCTGACAAT GACATTTTGC AGGAGATGAA TGGCAAGGCA 
GCGGAGCCGC CCACGCTTGA CTCGGAATCG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001245944.1
CDS	274..2226
Translation	MAASDKSVDDSLYPIAVLIDELKNEDVQLRLNSIKKLSTIALALGEERTRSELIPFLTETIYDEDEVLLALADQLGNFTSLVGGPEFAMYLIPPLESLATVEETVVRDKAVESLRTVAAEHSAQDLEIHVVPTLQRLVSGDWFTSRTSACGLFSVCYPRVTQPVKAELRANFRKLCQDETPMVRRAAANKLGEFAKVVETEYLKSDLIPNFVQLAQDDQDSVRLLAVEACVSIAQLLPQDDVEHLVLPTLRQCASDSSWRVRYMVAEKFVDLQKAVGPEITRVDLVPAFQYLLKDAEAEVRAAVATKVKDFCANLDKVNQVQIILSSILPYVRDLVSDPNPHVKSALASVIMGLSPMLGAYQTVEQLLPLFLIQLKDECPEVRLNIISNLDCVNDVIGIQQLSQSLLPAIVELAEDSKWRVRLAIIEYMPALAGQLGQEFFDQKLRGLCMGWLNDHVYAIREAATLNMKKLVEQFGAPWAEQAIIPMILVMSRNKNYLHRMTCLFCLNVLAEVCGTDITTKLLLPTVLLLAADPVANVRFNVAKTLQKISPFLEASVIDAQVKPTLDKLNTDTDVDVKHFAAQAIAGIAAEMELNVFRTAIPPCMGAKIEAAMDSKLIVEPPRQPEAADNDILQEMNGKAAEPPTLDSES

Target ORF information:

RefSeq Version	NM_001259015.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster protein phosphatase 2A at 29B, transcript variant D (Pp2A-29B), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001259015.2

ORF Insert Sequence:

ATGGCAGCAA GCGACAAATC GGTCGACGAT TCACTATATC CCATTGCGGT TCTAATCGAT 
GAACTGAAAA ACGAGGACGT TCAGCTTCGG TTGAACTCCA TCAAGAAACT GTCCACCATT 
GCACTCGCTT TGGGCGAGGA GCGCACACGG TCCGAGTTGA TTCCCTTCCT CACCGAGACC 
ATATACGATG AGGACGAGGT ACTGCTGGCC CTGGCCGACC AACTGGGCAA CTTTACTAGT 
CTCGTTGGTG GGCCAGAGTT TGCCATGTAC TTGATTCCGC CCCTCGAGAG TTTGGCCACC 
GTAGAGGAAA CCGTGGTGCG AGACAAGGCT GTGGAATCTC TACGCACCGT GGCCGCTGAG 
CACAGCGCCC AGGATTTGGA GATCCATGTG GTGCCGACAC TGCAGCGATT GGTTTCCGGT 
GACTGGTTCA CCTCACGCAC CTCTGCCTGC GGCCTCTTCT CGGTCTGCTA TCCACGCGTC 
ACACAGCCAG TGAAGGCCGA GCTGCGCGCC AACTTCCGAA AGCTCTGCCA GGATGAGACA 
CCCATGGTGC GCCGTGCAGC GGCCAACAAG CTGGGCGAGT TTGCCAAGGT CGTTGAGACG 
GAGTATCTGA AGTCCGATTT GATTCCCAAC TTTGTCCAGC TGGCACAGGA TGATCAGGAC 
TCTGTCCGTC TGCTGGCTGT AGAGGCATGC GTAAGCATTG CCCAGCTGCT GCCTCAGGAT 
GATGTAGAGC ACCTGGTTCT GCCCACGCTG CGCCAGTGCG CCAGCGACTC TTCCTGGAGG 
GTGCGTTACA TGGTGGCCGA GAAGTTTGTT GATCTGCAAA AGGCTGTGGG CCCAGAGATT 
ACTAGGGTGG ACTTGGTGCC TGCCTTCCAG TACTTGCTCA AGGATGCCGA GGCCGAGGTT 
CGCGCTGCAG TGGCCACCAA GGTGAAGGAC TTCTGCGCCA ATCTGGACAA GGTCAACCAG 
GTGCAAATCA TCCTTAGTTC CATTTTGCCC TATGTCCGCG ATCTTGTCTC GGACCCCAAT 
CCTCATGTGA AGTCAGCTCT GGCCTCAGTG ATCATGGGCT TGAGTCCCAT GCTGGGCGCC 
TATCAGACTG TGGAGCAATT GCTCCCCCTG TTCCTTATTC AACTCAAGGA TGAGTGCCCA 
GAAGTGCGCC TAAACATCAT CTCAAACCTG GATTGCGTTA ACGACGTCAT CGGTATCCAG 
CAACTGTCAC AGTCGCTTCT GCCCGCCATC GTCGAGCTGG CCGAGGACTC CAAGTGGCGT 
GTGCGTCTAG CCATCATCGA GTACATGCCT GCTCTGGCCG GTCAGTTGGG TCAGGAATTC 
TTTGACCAAA AACTGCGCGG TCTCTGCATG GGATGGCTCA ACGATCACGT GTACGCCATT 
CGTGAGGCAG CCACCCTCAA CATGAAGAAG CTCGTCGAGC AGTTCGGAGC TCCCTGGGCC 
GAACAGGCCA TAATTCCAAT GATTCTGGTT ATGTCGCGCA ACAAGAACTA TTTGCACAGA 
ATGACTTGCT TGTTCTGCCT GAATGTTTTG GCAGAGGTCT GCGGCACAGA TATCACCACC 
AAGTTGCTGC TGCCCACAGT TCTCCTGCTT GCCGCTGATC CCGTTGCCAA TGTTCGTTTC 
AACGTGGCAA AGACCCTGCA GAAGATCTCG CCCTTCCTGG AGGCCAGCGT CATTGATGCC 
CAAGTAAAGC CCACACTCGA CAAACTGAAC ACAGACACAG ATGTGGATGT CAAGCATTTT 
GCTGCACAGG CCATTGCCGG CATAGCTGCA GAAATGGAAC TGAATGTGTT TAGAACGGCA 
ATACCGCCGT GCATGGGCGC CAAAATTGAG GCGGCAATGG ACTCAAAGCT GATAGTGGAA 
CCGCCCCGAC AGCCGGAGGC AGCTGACAAT GACATTTTGC AGGAGATGAA TGGCAAGGCA 
GCGGAGCCGC CCACGCTTGA CTCGGAATCG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pp2A-29B ( NM_001298819.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001285748.1 Drosophila melanogaster protein phosphatase 2A at 29B, transcript variant E (Pp2A-29B), mRNA.

Price & Availability↑

CloneID	OFa22360
Clone ID Related Accession (Same CDS sequence)	NM_001298819.1
Accession Version	NM_001298819.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1953bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	protein phosphatase 2A at 29B, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCAGCAA GCGACAAATC GGTCGACGAT TCACTATATC CCATTGCGGT TCTAATCGAT 
GAACTGAAAA ACGAGGACGT TCAGCTTCGG TTGAACTCCA TCAAGAAACT GTCCACCATT 
GCACTCGCTT TGGGCGAGGA GCGCACACGG TCCGAGTTGA TTCCCTTCCT CACCGAGACC 
ATATACGATG AGGACGAGGT ACTGCTGGCC CTGGCCGACC AACTGGGCAA CTTTACTAGT 
CTCGTTGGTG GGCCAGAGTT TGCCATGTAC TTGATTCCGC CCCTCGAGAG TTTGGCCACC 
GTAGAGGAAA CCGTGGTGCG AGACAAGGCT GTGGAATCTC TACGCACCGT GGCCGCTGAG 
CACAGCGCCC AGGATTTGGA GATCCATGTG GTGCCGACAC TGCAGCGATT GGTTTCCGGT 
GACTGGTTCA CCTCACGCAC CTCTGCCTGC GGCCTCTTCT CGGTCTGCTA TCCACGCGTC 
ACACAGCCAG TGAAGGCCGA GCTGCGCGCC AACTTCCGAA AGCTCTGCCA GGATGAGACA 
CCCATGGTGC GCCGTGCAGC GGCCAACAAG CTGGGCGAGT TTGCCAAGGT CGTTGAGACG 
GAGTATCTGA AGTCCGATTT GATTCCCAAC TTTGTCCAGC TGGCACAGGA TGATCAGGAC 
TCTGTCCGTC TGCTGGCTGT AGAGGCATGC GTAAGCATTG CCCAGCTGCT GCCTCAGGAT 
GATGTAGAGC ACCTGGTTCT GCCCACGCTG CGCCAGTGCG CCAGCGACTC TTCCTGGAGG 
GTGCGTTACA TGGTGGCCGA GAAGTTTGTT GATCTGCAAA AGGCTGTGGG CCCAGAGATT 
ACTAGGGTGG ACTTGGTGCC TGCCTTCCAG TACTTGCTCA AGGATGCCGA GGCCGAGGTT 
CGCGCTGCAG TGGCCACCAA GGTGAAGGAC TTCTGCGCCA ATCTGGACAA GGTCAACCAG 
GTGCAAATCA TCCTTAGTTC CATTTTGCCC TATGTCCGCG ATCTTGTCTC GGACCCCAAT 
CCTCATGTGA AGTCAGCTCT GGCCTCAGTG ATCATGGGCT TGAGTCCCAT GCTGGGCGCC 
TATCAGACTG TGGAGCAATT GCTCCCCCTG TTCCTTATTC AACTCAAGGA TGAGTGCCCA 
GAAGTGCGCC TAAACATCAT CTCAAACCTG GATTGCGTTA ACGACGTCAT CGGTATCCAG 
CAACTGTCAC AGTCGCTTCT GCCCGCCATC GTCGAGCTGG CCGAGGACTC CAAGTGGCGT 
GTGCGTCTAG CCATCATCGA GTACATGCCT GCTCTGGCCG GTCAGTTGGG TCAGGAATTC 
TTTGACCAAA AACTGCGCGG TCTCTGCATG GGATGGCTCA ACGATCACGT GTACGCCATT 
CGTGAGGCAG CCACCCTCAA CATGAAGAAG CTCGTCGAGC AGTTCGGAGC TCCCTGGGCC 
GAACAGGCCA TAATTCCAAT GATTCTGGTT ATGTCGCGCA ACAAGAACTA TTTGCACAGA 
ATGACTTGCT TGTTCTGCCT GAATGTTTTG GCAGAGGTCT GCGGCACAGA TATCACCACC 
AAGTTGCTGC TGCCCACAGT TCTCCTGCTT GCCGCTGATC CCGTTGCCAA TGTTCGTTTC 
AACGTGGCAA AGACCCTGCA GAAGATCTCG CCCTTCCTGG AGGCCAGCGT CATTGATGCC 
CAAGTAAAGC CCACACTCGA CAAACTGAAC ACAGACACAG ATGTGGATGT CAAGCATTTT 
GCTGCACAGG CCATTGCCGG CATAGCTGCA GAAATGGAAC TGAATGTGTT TAGAACGGCA 
ATACCGCCGT GCATGGGCGC CAAAATTGAG GCGGCAATGG ACTCAAAGCT GATAGTGGAA 
CCGCCCCGAC AGCCGGAGGC AGCTGACAAT GACATTTTGC AGGAGATGAA TGGCAAGGCA 
GCGGAGCCGC CCACGCTTGA CTCGGAATGT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001285748.1
CDS	274..2226
Translation	MAASDKSVDDSLYPIAVLIDELKNEDVQLRLNSIKKLSTIALALGEERTRSELIPFLTETIYDEDEVLLALADQLGNFTSLVGGPEFAMYLIPPLESLATVEETVVRDKAVESLRTVAAEHSAQDLEIHVVPTLQRLVSGDWFTSRTSACGLFSVCYPRVTQPVKAELRANFRKLCQDETPMVRRAAANKLGEFAKVVETEYLKSDLIPNFVQLAQDDQDSVRLLAVEACVSIAQLLPQDDVEHLVLPTLRQCASDSSWRVRYMVAEKFVDLQKAVGPEITRVDLVPAFQYLLKDAEAEVRAAVATKVKDFCANLDKVNQVQIILSSILPYVRDLVSDPNPHVKSALASVIMGLSPMLGAYQTVEQLLPLFLIQLKDECPEVRLNIISNLDCVNDVIGIQQLSQSLLPAIVELAEDSKWRVRLAIIEYMPALAGQLGQEFFDQKLRGLCMGWLNDHVYAIREAATLNMKKLVEQFGAPWAEQAIIPMILVMSRNKNYLHRMTCLFCLNVLAEVCGTDITTKLLLPTVLLLAADPVANVRFNVAKTLQKISPFLEASVIDAQVKPTLDKLNTDTDVDVKHFAAQAIAGIAAEMELNVFRTAIPPCMGAKIEAAMDSKLIVEPPRQPEAADNDILQEMNGKAAEPPTLDSEC

Target ORF information:

RefSeq Version	NM_001298819.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster protein phosphatase 2A at 29B, transcript variant E (Pp2A-29B), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001298819.1

ORF Insert Sequence:

ATGGCAGCAA GCGACAAATC GGTCGACGAT TCACTATATC CCATTGCGGT TCTAATCGAT 
GAACTGAAAA ACGAGGACGT TCAGCTTCGG TTGAACTCCA TCAAGAAACT GTCCACCATT 
GCACTCGCTT TGGGCGAGGA GCGCACACGG TCCGAGTTGA TTCCCTTCCT CACCGAGACC 
ATATACGATG AGGACGAGGT ACTGCTGGCC CTGGCCGACC AACTGGGCAA CTTTACTAGT 
CTCGTTGGTG GGCCAGAGTT TGCCATGTAC TTGATTCCGC CCCTCGAGAG TTTGGCCACC 
GTAGAGGAAA CCGTGGTGCG AGACAAGGCT GTGGAATCTC TACGCACCGT GGCCGCTGAG 
CACAGCGCCC AGGATTTGGA GATCCATGTG GTGCCGACAC TGCAGCGATT GGTTTCCGGT 
GACTGGTTCA CCTCACGCAC CTCTGCCTGC GGCCTCTTCT CGGTCTGCTA TCCACGCGTC 
ACACAGCCAG TGAAGGCCGA GCTGCGCGCC AACTTCCGAA AGCTCTGCCA GGATGAGACA 
CCCATGGTGC GCCGTGCAGC GGCCAACAAG CTGGGCGAGT TTGCCAAGGT CGTTGAGACG 
GAGTATCTGA AGTCCGATTT GATTCCCAAC TTTGTCCAGC TGGCACAGGA TGATCAGGAC 
TCTGTCCGTC TGCTGGCTGT AGAGGCATGC GTAAGCATTG CCCAGCTGCT GCCTCAGGAT 
GATGTAGAGC ACCTGGTTCT GCCCACGCTG CGCCAGTGCG CCAGCGACTC TTCCTGGAGG 
GTGCGTTACA TGGTGGCCGA GAAGTTTGTT GATCTGCAAA AGGCTGTGGG CCCAGAGATT 
ACTAGGGTGG ACTTGGTGCC TGCCTTCCAG TACTTGCTCA AGGATGCCGA GGCCGAGGTT 
CGCGCTGCAG TGGCCACCAA GGTGAAGGAC TTCTGCGCCA ATCTGGACAA GGTCAACCAG 
GTGCAAATCA TCCTTAGTTC CATTTTGCCC TATGTCCGCG ATCTTGTCTC GGACCCCAAT 
CCTCATGTGA AGTCAGCTCT GGCCTCAGTG ATCATGGGCT TGAGTCCCAT GCTGGGCGCC 
TATCAGACTG TGGAGCAATT GCTCCCCCTG TTCCTTATTC AACTCAAGGA TGAGTGCCCA 
GAAGTGCGCC TAAACATCAT CTCAAACCTG GATTGCGTTA ACGACGTCAT CGGTATCCAG 
CAACTGTCAC AGTCGCTTCT GCCCGCCATC GTCGAGCTGG CCGAGGACTC CAAGTGGCGT 
GTGCGTCTAG CCATCATCGA GTACATGCCT GCTCTGGCCG GTCAGTTGGG TCAGGAATTC 
TTTGACCAAA AACTGCGCGG TCTCTGCATG GGATGGCTCA ACGATCACGT GTACGCCATT 
CGTGAGGCAG CCACCCTCAA CATGAAGAAG CTCGTCGAGC AGTTCGGAGC TCCCTGGGCC 
GAACAGGCCA TAATTCCAAT GATTCTGGTT ATGTCGCGCA ACAAGAACTA TTTGCACAGA 
ATGACTTGCT TGTTCTGCCT GAATGTTTTG GCAGAGGTCT GCGGCACAGA TATCACCACC 
AAGTTGCTGC TGCCCACAGT TCTCCTGCTT GCCGCTGATC CCGTTGCCAA TGTTCGTTTC 
AACGTGGCAA AGACCCTGCA GAAGATCTCG CCCTTCCTGG AGGCCAGCGT CATTGATGCC 
CAAGTAAAGC CCACACTCGA CAAACTGAAC ACAGACACAG ATGTGGATGT CAAGCATTTT 
GCTGCACAGG CCATTGCCGG CATAGCTGCA GAAATGGAAC TGAATGTGTT TAGAACGGCA 
ATACCGCCGT GCATGGGCGC CAAAATTGAG GCGGCAATGG ACTCAAAGCT GATAGTGGAA 
CCGCCCCGAC AGCCGGAGGC AGCTGACAAT GACATTTTGC AGGAGATGAA TGGCAAGGCA 
GCGGAGCCGC CCACGCTTGA CTCGGAATGT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Pp2A-29B cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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