ppk25 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	ppk25
Entrez Gene ID	2768722
Full Name	pickpocket 25
Synonyms	CG15242,CG33349,Dmel\CG33349,llz
General protein information	Preferred Names pickpocket 25 Names CG33349 gene product from transcript CG33349-RA CG33349-PA lounge lizard ppk25-PA
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 42E1-42E1

mRNA and Protein(s)

mRNA	Protein	Name
NM_206044.1	NP_995766.1	pickpocket 25

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	ppk25	NP_995766.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005516	XP_315517.3

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

While ppk25 neurons are required for the responses to multiple pheromones, in both males and females these neurons are specifically involved in stimulating courtship and mating.
Title: Drosophila pheromone-sensing neurons expressing the ppk25 ion channel subunit stimulate male courtship and female receptivity.

This study indicated that two DEG/ENaC subunits, Nope and Ppk25, are coexpressed and have specific, nonredundant functions in a common subset of courtship-activating gustatory neurons in the wings and legs of males
Title: Two Drosophila DEG/ENaC channel subunits have distinct functions in gustatory neurons that activate male courtship.

These data show that ppk25 functions specifically in peripheral taste neurons involved in activation of courtship behavior.
Title: A Drosophila DEG/ENaC subunit functions specifically in gustatory neurons required for male courtship behavior.

Data show that when coexpressed, LLZ coprecipitated with CHEB42A, suggesting that the two proteins form a complex.
Title: The Drosophila gene CheB42a is a novel modifier of Deg/ENaC channel function.

ppk25 is necessary for response to female pheromones by D. melanogaster males
Title: A Drosophila DEG/ENaC channel subunit is required for male response to female pheromones.

The following ppk25 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ppk25 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa11773	NM_206044.1 Latest version!	Drosophila melanogaster pickpocket 25 (ppk25), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ppk25 ( NM_206044.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_995766.1 Drosophila melanogaster pickpocket 25 (ppk25), partial mRNA.

Price & Availability↑

CloneID	OFa11773
Clone ID Related Accession (Same CDS sequence)	NM_206044.1
Accession Version	NM_206044.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1356bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	pickpocket 25
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on the 3' end.

ATGGAATCCA AAATGAGTCG GCCGAAGCAA CCGGACTGGC TCGCCGAGCT GTGCCAGGAG 
TCCTCCATCC ACGGCATGCC CTACATCGCC CGCAGGGATC TACACTGGGC CGAGCGCCTC 
TTTTGGACAT TCATAATCCT GGGCTCGGCC TACTACGCCA TCAGCAGCTG CCTTAACCAA 
TGGTACCGGT TCCGGGACAA TCCTATTGTC TACGAGTACG AATATCTCTT TGGGCTGCGC 
ATCTTCCCCT TCGTGGGGAT AACACTGTGC CCTAGGTACC ACGACGAAAC GGAGATCCCA 
AGGCTTATAA ATCAAACTTG GGGAGTGGAT CCCAGCGAAG ACAAAGAAAA AGCTGTGTAC 
TACAGAAAGT TTCTGCTTGC AATCAACGGC CTTCGTTACT CCACTCTGGA GACGCTGGAA 
CCCTTCGAGA ATGATACCAC TTTGGACAAC GTGAATTACT TGAACATTTT GCTCACGCTG 
CAAAAAAAGG TAATAGCGGT TAAAATCCCG CCAGAACTGG CGCCAATCAT TACTGAGGTG 
GGTCTGTGCC AAACATCCAG CCAGTTAAAC CGATACGGAA ATCCCTACGG CAAACTAGAA 
ACACAGGATA TGGAACCCAT GAAACAGTGC GGCTATTTGA GTAATTGCAT CACATCTCTG 
AAGCCTATAA ATAGCATTGT TGCCCCCATA TTTATGTACC TTCACGATGT CGAAGAAATG 
ATGCTACCCG ACGACATGCG AACTCCCTCT TTTGATGCTA AAGACATCGA ATCCAAGGAT 
CTCGATATTA TGCTACATAC CACATCGGCG GAAAGCGAGG TGCGAAATCT ACCGGTAGCA 
TATCGCAAGT GTCGCTTCAG TGACGAAAAC AATCTGCAGT ATTACAGTCC CTACCACCCA 
AGTCTTTGCC GACTTGAGTG CCGAATCAAA TGGGCCCTGA GCCTGTGCAA CTGCAAGCCC 
TATTTCTACG TAGCAGCTCC CGAAGTTCCA ATCTGCACAG TATCCGGAAT GCTCTGCCTA 
GCCCGGTCCA AGTGGCTTGA AAGACCATGC GATTGCTATC CGTCTTGTCG CGAGGAAACC 
TTTACCATCT TCAAAGTGTC TGACCAGACT GGGGGCGATG ACAACTACTC TGGCGAGAGG 
TTCGAGCGGA CGCTGATCAT CAACCTGCAA ATTTCGAGGA TGGGCATAAA TCGGCGGGTT 
GTATTCAGCA CGGATCAGTT GATAATGTCG TTTGGTGGAG CCATTGGCCT CTTTCTTGGA 
GCCAGCTTCA TGACCATATA CGGCGTGGTA TACTTTTTTC TAACTTTCAT AGCTTATACA 
TGCAAGAACC GATTTTGCAA GCGTTTTTTT TTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_995766.1
CDS	7..1362
Translation	MESKMSRPKQPDWLAELCQESSIHGMPYIARRDLHWAERLFWTFIILGSAYYAISSCLNQWYRFRDNPIVYEYEYLFGLRIFPFVGITLCPRYHDETEIPRLINQTWGVDPSEDKEKAVYYRKFLLAINGLRYSTLETLEPFENDTTLDNVNYLNILLTLQKKVIAVKIPPELAPIITEVGLCQTSSQLNRYGNPYGKLETQDMEPMKQCGYLSNCITSLKPINSIVAPIFMYLHDVEEMMLPDDMRTPSFDAKDIESKDLDIMLHTTSAESEVRNLPVAYRKCRFSDENNLQYYSPYHPSLCRLECRIKWALSLCNCKPYFYVAAPEVPICTVSGMLCLARSKWLERPCDCYPSCREETFTIFKVSDQTGGDDNYSGERFERTLIINLQISRMGINRRVVFSTDQLIMSFGGAIGLFLGASFMTIYGVVYFFLTFIAYTCKNRFCKRFFF

Target ORF information:

RefSeq Version	NM_206044.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster pickpocket 25 (ppk25), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_206044.1

ORF Insert Sequence:

ATGGAATCCA AAATGAGTCG GCCGAAGCAA CCGGACTGGC TCGCCGAGCT GTGCCAGGAG 
TCCTCCATCC ACGGCATGCC CTACATCGCC CGCAGGGATC TACACTGGGC CGAGCGCCTC 
TTTTGGACAT TCATAATCCT GGGCTCGGCC TACTACGCCA TCAGCAGCTG CCTTAACCAA 
TGGTACCGGT TCCGGGACAA TCCTATTGTC TACGAGTACG AATATCTCTT TGGGCTGCGC 
ATCTTCCCCT TCGTGGGGAT AACACTGTGC CCTAGGTACC ACGACGAAAC GGAGATCCCA 
AGGCTTATAA ATCAAACTTG GGGAGTGGAT CCCAGCGAAG ACAAAGAAAA AGCTGTGTAC 
TACAGAAAGT TTCTGCTTGC AATCAACGGC CTTCGTTACT CCACTCTGGA GACGCTGGAA 
CCCTTCGAGA ATGATACCAC TTTGGACAAC GTGAATTACT TGAACATTTT GCTCACGCTG 
CAAAAAAAGG TAATAGCGGT TAAAATCCCG CCAGAACTGG CGCCAATCAT TACTGAGGTG 
GGTCTGTGCC AAACATCCAG CCAGTTAAAC CGATACGGAA ATCCCTACGG CAAACTAGAA 
ACACAGGATA TGGAACCCAT GAAACAGTGC GGCTATTTGA GTAATTGCAT CACATCTCTG 
AAGCCTATAA ATAGCATTGT TGCCCCCATA TTTATGTACC TTCACGATGT CGAAGAAATG 
ATGCTACCCG ACGACATGCG AACTCCCTCT TTTGATGCTA AAGACATCGA ATCCAAGGAT 
CTCGATATTA TGCTACATAC CACATCGGCG GAAAGCGAGG TGCGAAATCT ACCGGTAGCA 
TATCGCAAGT GTCGCTTCAG TGACGAAAAC AATCTGCAGT ATTACAGTCC CTACCACCCA 
AGTCTTTGCC GACTTGAGTG CCGAATCAAA TGGGCCCTGA GCCTGTGCAA CTGCAAGCCC 
TATTTCTACG TAGCAGCTCC CGAAGTTCCA ATCTGCACAG TATCCGGAAT GCTCTGCCTA 
GCCCGGTCCA AGTGGCTTGA AAGACCATGC GATTGCTATC CGTCTTGTCG CGAGGAAACC 
TTTACCATCT TCAAAGTGTC TGACCAGACT GGGGGCGATG ACAACTACTC TGGCGAGAGG 
TTCGAGCGGA CGCTGATCAT CAACCTGCAA ATTTCGAGGA TGGGCATAAA TCGGCGGGTT 
GTATTCAGCA CGGATCAGTT GATAATGTCG TTTGGTGGAG CCATTGGCCT CTTTCTTGGA 
GCCAGCTTCA TGACCATATA CGGCGTGGTA TACTTTTTTC TAACTTTCAT AGCTTATACA 
TGCAAGAACC GATTTTGCAA GCGTTTTTTT TTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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ppk25 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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