bwa cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	bwa
Entrez Gene ID	250736
Full Name	brain washing
Synonyms	38B.6,CG13969,Dmel\CG13969,dacer
General protein information	Preferred Names brain washing Names CG13969 gene product from transcript CG13969-RA CG13969-PA CG13969-PB brainwashing bwa-PA bwa-PB
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 38B2-38B3

mRNA and Protein(s)

mRNA	Protein	Name
NM_001273687.2	NP_001260616.1	brain washing, isoform B
NM_136176.4	NP_610020.1	brain washing, isoform A

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	ACER2	XP_003639431.1
Bos taurus (cattle)	ACER2	NP_001179704.1
Homo sapiens (human)	ACER2	NP_001010887.2
Mus musculus (house mouse)	Acer2	NP_647467.1
Gallus gallus (chicken)	ACER2	XP_424820.3
Danio rerio (zebrafish)	LOC100331179	XP_002663077.2
Drosophila melanogaster (fruit fly)	bwa	NP_001260616.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008729	XP_314841.2
Xenopus tropicalis (tropical clawed frog)	acer2	NP_001017116.1
Pan troglodytes (chimpanzee)	ACER2	XP_520508.2
Rattus norvegicus (Norway rat)	Acer2	NP_001101413.1
Caenorhabditis elegans (roundworm)	CELE_W02F12.2	NP_504697.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

study demonstrated that BWA does not exhibit ceramidase activity; although BWA is unlikely to be a ceramidase, it is a regulator of sphingolipid flux; Bwa exhibits strong genetic interaction with other genes coding for ceramide-metabolizing enzymes
Title: CDase is a pan-ceramidase in Drosophila.

The following bwa gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the bwa cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa15385	NM_136176.4 Latest version!	Drosophila melanogaster brain washing (bwa), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OFa15793	NM_001273687.2 Latest version!	Drosophila melanogaster brain washing (bwa), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

bwa ( NM_136176.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_610020.1 Drosophila melanogaster brain washing (bwa), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa15385
Clone ID Related Accession (Same CDS sequence)	NM_136176.4
Accession Version	NM_136176.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 852bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	brain washing, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_136176.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGCGGGA TGGGGGGCGG CGGGCTGCTG GACATCTACG CCATGGCCTG GGAGCACCTA 
AGACCCGGAA GCTCGCCCGT CGACTGGTGC GAAGGCAACT ACTTGATTTC GTCCAACATC 
GCCGAGTTCG TGAACACGTT TAGCAACTTC CTGTTCATCC TACTGCCGCC CGTCCTTATA 
ATGCTCTTCA AGGAGTACGG ACGCTTTGTG ACGCCCGGAA TCCACGTCAT CTGGGTGCTG 
CTCATCGTGG TTGGCCTGAG TTCGATGTAC TTCCATGCCA CTTTGAGTCT GATTGGCCAG 
CTGCTGGACG AACTGGCCAT ACTCTGGGTC TTCATGGCGG CCTTTTCGCT CTTCTATCCG 
AAGCGATACT ATCCCAAGTT CGTGAAAAAC GATCGCAAAA CCTTCAGTTG GCTCATGCTC 
TTGTCGGCGA TTGCTGCGAC GGGCTTGTCG TGGTGGAAGC CCATTGTTAA CGCCTTTGTT 
CTCATGTTCA TGAGTGTGCC GACCATGGTA ATGCTCTACA CAGAGCTGCA GAGAGTTAGT 
GACCAGAGGG TCTACCGCCT GGGCATCCGA TCGACGACCG TCTGGGCTGT TGCGGTGTTC 
TGCTGGATCA ATGACCGGAT CTTCTGCGAG GCCTGGTCCT CGATCAATTT TCCATACCTG 
CACGGCTTCT GGCACATATT CATTTTTATA GCCGCCTACA CGGTGCTGGT GCTTTTCGCC 
TACTTCTACG TGGAATCGGA GCTGCCCCAG CGACAGCCGC TGCTGAAGTA CTGGCCAAAG 
AACGAGTTCG AGTTCGGGAT ACCCTTCATT TCGATCCGGA ATCCAGGTAA GGCTCTGCGC 
AACACCATCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_610020.1
CDS	371..1222
Translation	MGGMGGGGLLDIYAMAWEHLRPGSSPVDWCEGNYLISSNIAEFVNTFSNFLFILLPPVLIMLFKEYGRFVTPGIHVIWVLLIVVGLSSMYFHATLSLIGQLLDELAILWVFMAAFSLFYPKRYYPKFVKNDRKTFSWLMLLSAIAATGLSWWKPIVNAFVLMFMSVPTMVMLYTELQRVSDQRVYRLGIRSTTVWAVAVFCWINDRIFCEAWSSINFPYLHGFWHIFIFIAAYTVLVLFAYFYVESELPQRQPLLKYWPKNEFEFGIPFISIRNPGKALRNTI

Target ORF information:

RefSeq Version	NM_136176.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster brain washing (bwa), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_136176.4

ORF Insert Sequence:

ATGGGCGGGA TGGGGGGCGG CGGGCTGCTG GACATCTACG CCATGGCCTG GGAGCACCTA 
AGACCCGGAA GCTCGCCCGT CGACTGGTGC GAAGGCAACT ACTTGATTTC GTCCAACATC 
GCCGAGTTCG TGAACACGTT TAGCAACTTC CTGTTCATCC TACTGCCGCC CGTCCTTATA 
ATGCTCTTCA AGGAGTACGG ACGCTTTGTG ACGCCCGGAA TCCACGTCAT CTGGGTGCTG 
CTCATCGTGG TTGGCCTGAG TTCGATGTAC TTCCATGCCA CTTTGAGTCT GATTGGCCAG 
CTGCTGGACG AACTGGCCAT ACTCTGGGTC TTCATGGCGG CCTTTTCGCT CTTCTATCCG 
AAGCGATACT ATCCCAAGTT CGTGAAAAAC GATCGCAAAA CCTTCAGTTG GCTCATGCTC 
TTGTCGGCGA TTGCTGCGAC GGGCTTGTCG TGGTGGAAGC CCATTGTTAA CGCCTTTGTT 
CTCATGTTCA TGAGTGTGCC GACCATGGTA ATGCTCTACA CAGAGCTGCA GAGAGTTAGT 
GACCAGAGGG TCTACCGCCT GGGCATCCGA TCGACGACCG TCTGGGCTGT TGCGGTGTTC 
TGCTGGATCA ATGACCGGAT CTTCTGCGAG GCCTGGTCCT CGATCAATTT TCCATACCTG 
CACGGCTTCT GGCACATATT CATTTTTATA GCCGCCTACA CGGTGCTGGT GCTTTTCGCC 
TACTTCTACG TGGAATCGGA GCTGCCCCAG CGACAGCCGC TGCTGAAGTA CTGGCCAAAG 
AACGAGTTCG AGTTCGGGAT ACCCTTCATT TCGATCCGGA ATCCAGGTAA GGCTCTGCGC 
AACACCATCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

bwa ( NM_001273687.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001260616.1 Drosophila melanogaster brain washing (bwa), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa15793
Clone ID Related Accession (Same CDS sequence)	NM_001273687.2
Accession Version	NM_001273687.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 903bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	brain washing, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_001273687.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGCGGGA TGGGGGGCGG CGGGCTGCTG GACATCTACG CCATGGCCTG GGAGCACCTA 
AGACCCGGAA GCTCGCCCGT CGACTGGTGC GAAGGCAACT ACTTGATTTC GTCCAACATC 
GCCGAGTTCG TGAACACGTT TAGCAACTTC CTGTTCATCC TACTGCCGCC CGTCCTTATA 
ATGCTCTTCA AGGAGTACGG ACGCTTTGTG ACGCCCGGAA TCCACGTCAT CTGGGTGCTG 
CTCATCGTGG TTGGCCTGAG TTCGATGTAC TTCCATGCCA CTTTGAGTCT GATTGGCCAG 
CTGCTGGACG AACTGGCCAT ACTCTGGGTC TTCATGGCGG CCTTTTCGCT CTTCTATCCG 
AAGCGATACT ATCCCAAGTT CGTGAAAAAC GATCGCAAAA CCTTCAGTTG GCTCATGCTC 
TTGTCGGCGA TTGCTGCGAC GGGCTTGTCG TGGTGGAAGC CCATTGTTAA CGCCTTTGTT 
CTCATGTTCA TGAGTGTGCC GACCATGGTA ATGCTCTACA CAGAGCTGCA GAGAGTTAGT 
GACCAGAGGG TCTACCGCCT GGGCATCCGA TCGACGACCG TCTGGGCTGT TGCGGTGTTC 
TGCTGGATCA ATGACCGGAT CTTCTGCGAG GCCTGGTCCT CGATCAATTT TCCATACCTG 
CACGGCTTCT GGCACATATT CATTTTTATA GCCGCCTACA CGGTGCTGGT GCTTTTCGCC 
TACTTCTACG TGGAATCGGA GCTGCCCCAG CGACAGCCGC TGCTGAAGTA CTGGCCAAAG 
AACGAGTTCG AGTTCGGGAT ACCCTTCATT TCGATCCGGA ATCCAGATTC GAAAGATTGG 
CTCGTCTACT GCAGTCTGTA CATGCAGGAC TATGACTATG ACTACAAGCA CCGAAAGAAC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001260616.1
CDS	371..1273
Translation	MGGMGGGGLLDIYAMAWEHLRPGSSPVDWCEGNYLISSNIAEFVNTFSNFLFILLPPVLIMLFKEYGRFVTPGIHVIWVLLIVVGLSSMYFHATLSLIGQLLDELAILWVFMAAFSLFYPKRYYPKFVKNDRKTFSWLMLLSAIAATGLSWWKPIVNAFVLMFMSVPTMVMLYTELQRVSDQRVYRLGIRSTTVWAVAVFCWINDRIFCEAWSSINFPYLHGFWHIFIFIAAYTVLVLFAYFYVESELPQRQPLLKYWPKNEFEFGIPFISIRNPDSKDWLVYCSLYMQDYDYDYKHRKN

Target ORF information:

RefSeq Version	NM_001273687.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster brain washing (bwa), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001273687.2

ORF Insert Sequence:

ATGGGCGGGA TGGGGGGCGG CGGGCTGCTG GACATCTACG CCATGGCCTG GGAGCACCTA 
AGACCCGGAA GCTCGCCCGT CGACTGGTGC GAAGGCAACT ACTTGATTTC GTCCAACATC 
GCCGAGTTCG TGAACACGTT TAGCAACTTC CTGTTCATCC TACTGCCGCC CGTCCTTATA 
ATGCTCTTCA AGGAGTACGG ACGCTTTGTG ACGCCCGGAA TCCACGTCAT CTGGGTGCTG 
CTCATCGTGG TTGGCCTGAG TTCGATGTAC TTCCATGCCA CTTTGAGTCT GATTGGCCAG 
CTGCTGGACG AACTGGCCAT ACTCTGGGTC TTCATGGCGG CCTTTTCGCT CTTCTATCCG 
AAGCGATACT ATCCCAAGTT CGTGAAAAAC GATCGCAAAA CCTTCAGTTG GCTCATGCTC 
TTGTCGGCGA TTGCTGCGAC GGGCTTGTCG TGGTGGAAGC CCATTGTTAA CGCCTTTGTT 
CTCATGTTCA TGAGTGTGCC GACCATGGTA ATGCTCTACA CAGAGCTGCA GAGAGTTAGT 
GACCAGAGGG TCTACCGCCT GGGCATCCGA TCGACGACCG TCTGGGCTGT TGCGGTGTTC 
TGCTGGATCA ATGACCGGAT CTTCTGCGAG GCCTGGTCCT CGATCAATTT TCCATACCTG 
CACGGCTTCT GGCACATATT CATTTTTATA GCCGCCTACA CGGTGCTGGT GCTTTTCGCC 
TACTTCTACG TGGAATCGGA GCTGCCCCAG CGACAGCCGC TGCTGAAGTA CTGGCCAAAG 
AACGAGTTCG AGTTCGGGAT ACCCTTCATT TCGATCCGGA ATCCAGATTC GAAAGATTGG 
CTCGTCTACT GCAGTCTGTA CATGCAGGAC TATGACTATG ACTACAAGCA CCGAAAGAAC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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bwa cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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