HSPC300 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	HSPC300
Entrez Gene ID	246497
Full Name	Haematopoietic stem/progenitor cell protein 300
Synonyms	Brk1,CG30173,Dmel\CG30173,SIP1,dHSPC,dHSPC300
General protein information	Preferred Names Haematopoietic stem/progenitor cell protein 300 Names CG30173 gene product from transcript CG30173-RB CG30173-PA CG30173-PB HSPC300-PA HSPC300-PB haematopoietic stem/progenitor cell protein 300 syntaxin interacting protein 1
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 60B4-60B4

mRNA and Protein(s)

mRNA	Protein	Name
NM_166648.3	NP_726400.1	haematopoietic stem/progenitor cell protein 300, isoform A
NM_001299881.1	NP_001286810.1	haematopoietic stem/progenitor cell protein 300, isoform B

Pathways from BioSystems

Homologies

Bos taurus (cattle)	BRK1	NP_001107982.1
Danio rerio (zebrafish)	zgc:86903	NP_001002097.1
Rattus norvegicus (Norway rat)	Brk1	NP_001182405.1
Xenopus tropicalis (tropical clawed frog)	brk1	NP_001015994.1
Pan troglodytes (chimpanzee)	BRK1	XP_003950077.1
	LOC720448	XP_002806005.1
Canis lupus familiaris (dog)	LOC607701	XP_005632227.1
Mus musculus (house mouse)	Brk1	NP_598698.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007614	XP_308258.2
Homo sapiens (human)	BRK1	NP_060932.2
	LOC717243	XP_002799727.1
Drosophila melanogaster (fruit fly)	HSPC300	NP_726400.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

HSPC300 plays a role in nervous system development and functions in the context of the WAVE/SCAR complex.
Title: HSPC300 and its role in neuronal connectivity.

The following HSPC300 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HSPC300 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa11623	NM_166648.3 Latest version!	Drosophila melanogaster haematopoietic stem/progenitor cell protein 300, transcript variant A (HSPC300), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OFa11623	NM_001299881.1 Latest version!	Drosophila melanogaster haematopoietic stem/progenitor cell protein 300, transcript variant B (HSPC300), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

HSPC300 ( NM_166648.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_726400.1 Drosophila melanogaster haematopoietic stem/progenitor cell protein 300, transcript variant A (HSPC300), mRNA.

Price & Availability↑

CloneID	OFa11623
Clone ID Related Accession (Same CDS sequence)	NM_166648.3 , NM_001299881.1
Accession Version	NM_166648.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 231bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	haematopoietic stem/progenitor cell protein 300, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_166648.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGTGGGG CTCACAGAGA GGCGATCCAA AAGCAGATCC ACCAGGACTG GGCGAACAGG 
GAGTATATCG AAGTGATAAC TGCCAGCATA AAGAGAATCA CCGACTTTCT GAACTCTTTC 
GATATGTCCT GTCGCTCCCG TCTGGCGGTG CTGAACGAAA AACTAACGAT CCTGGAGCGG 
CGCATAGACT ATCTGGAGGC GTGCGTCGCC CAGGGTGAAA CATTAACGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_726400.1
CDS	56..286
Translation	MSGAHREAIQKQIHQDWANREYIEVITASIKRITDFLNSFDMSCRSRLAVLNEKLTILERRIDYLEACVAQGETLT

Target ORF information:

RefSeq Version	NM_166648.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster haematopoietic stem/progenitor cell protein 300, transcript variant A (HSPC300), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_166648.3

ORF Insert Sequence:

ATGAGTGGGG CTCACAGAGA GGCGATCCAA AAGCAGATCC ACCAGGACTG GGCGAACAGG 
GAGTATATCG AAGTGATAAC TGCCAGCATA AAGAGAATCA CCGACTTTCT GAACTCTTTC 
GATATGTCCT GTCGCTCCCG TCTGGCGGTG CTGAACGAAA AACTAACGAT CCTGGAGCGG 
CGCATAGACT ATCTGGAGGC GTGCGTCGCC CAGGGTGAAA CATTAACGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HSPC300 ( NM_001299881.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001286810.1 Drosophila melanogaster haematopoietic stem/progenitor cell protein 300, transcript variant B (HSPC300), mRNA.

Price & Availability↑

CloneID	OFa11623
Clone ID Related Accession (Same CDS sequence)	NM_166648.3 , NM_001299881.1
Accession Version	NM_001299881.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 231bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	haematopoietic stem/progenitor cell protein 300, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGTGGGG CTCACAGAGA GGCGATCCAA AAGCAGATCC ACCAGGACTG GGCGAACAGG 
GAGTATATCG AAGTGATAAC TGCCAGCATA AAGAGAATCA CCGACTTTCT GAACTCTTTC 
GATATGTCCT GTCGCTCCCG TCTGGCGGTG CTGAACGAAA AACTAACGAT CCTGGAGCGG 
CGCATAGACT ATCTGGAGGC GTGCGTCGCC CAGGGTGAAA CATTAACGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001286810.1
CDS	56..286
Translation	MSGAHREAIQKQIHQDWANREYIEVITASIKRITDFLNSFDMSCRSRLAVLNEKLTILERRIDYLEACVAQGETLT

Target ORF information:

RefSeq Version	NM_001299881.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster haematopoietic stem/progenitor cell protein 300, transcript variant B (HSPC300), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001299881.1

ORF Insert Sequence:

ATGAGTGGGG CTCACAGAGA GGCGATCCAA AAGCAGATCC ACCAGGACTG GGCGAACAGG 
GAGTATATCG AAGTGATAAC TGCCAGCATA AAGAGAATCA CCGACTTTCT GAACTCTTTC 
GATATGTCCT GTCGCTCCCG TCTGGCGGTG CTGAACGAAA AACTAACGAT CCTGGAGCGG 
CGCATAGACT ATCTGGAGGC GTGCGTCGCC CAGGGTGAAA CATTAACGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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HSPC300 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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