Ndf cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ndf
Entrez Gene ID	192507
Full Name	CG4747 gene product from transcript CG4747-RB
Synonyms	BEST:GH19401,BEST:LD22483,BEST:LD29743,Dm_AI388468,Dmel\CG4747,LD29743
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 30F5-30F5

mRNA and Protein(s)

mRNA	Protein	Name
NM_001298862.1	NP_001285791.1	Nucleosome-destabilizing factor, isoform C
NM_001201838.2	NP_001188767.1	Nucleosome-destabilizing factor, isoform B
NM_135492.5	NP_609336.3	Nucleosome-destabilizing factor, isoform A

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Glyr1	NP_001007801.1
Drosophila melanogaster (fruit fly)	CG4747	NP_001188767.1
Oryza sativa (rice)	Os02g0562700	NP_001047154.1
Xenopus tropicalis (tropical clawed frog)	glyr1	NP_001025665.1
	GLYR1	XP_001099732.2
Canis lupus familiaris (dog)	GLYR1	XP_851659.3
Gallus gallus (chicken)	GLYR1	NP_001006572.1
Anopheles gambiae (African malaria mosquito)	GLYR1_ANOGA	XP_319082.4
Mus musculus (house mouse)	Glyr1	NP_001073282.1
Arabidopsis thaliana (thale cress)	GLYR1	NP_566768.1
Danio rerio (zebrafish)	glyr1	NP_001007772.1
Homo sapiens (human)	GLYR1	NP_115958.2
Pan troglodytes (chimpanzee)	GLYR1	XP_001169777.3
Bos taurus (cattle)	GLYR1	NP_001035658.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

CG4747 and Set2 function together to facilitate targeting of the male-specific lethal complex to active genes.
Title: Chromatin proteins captured by ChIP-mass spectrometry are linked to dosage compensation in Drosophila.

The following Ndf gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ndf cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa15377	NM_001298862.1 Latest version!	Drosophila melanogaster Nucleosome-destabilizing factor (Ndf), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa15377	NM_135492.5 Latest version!	Drosophila melanogaster Nucleosome-destabilizing factor (Ndf), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa15788	NM_001201838.2 Latest version!	Drosophila melanogaster Nucleosome-destabilizing factor (Ndf), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ndf ( NM_001298862.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001285791.1 Drosophila melanogaster Nucleosome-destabilizing factor (Ndf), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa15377
Clone ID Related Accession (Same CDS sequence)	NM_001298862.1 , NM_135492.5
Accession Version	NM_001298862.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1809bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Nucleosome-destabilizing factor, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGAAGA ATAAAAAACT GTCGCTGTCG GGCGGCGATA CGACGGAGAA ATTTATTTAC 
AAACCCAAGG ATCTGATATG GGCCAAAATG AAGGGCTTCA CTCCGTGGCC AGGAATGATT 
GTCGATCCTC CACTTGATCT GCTCAGCCAG CAGCGTCGGG CGAATACCAA ATGCGTGTTT 
TTCTTTGGAT CTAGGAATTT TGCCTGGATT GAGGAGAACA ACATCAAGCC CTTCGAAGGA 
CCGTGGAAGG AGGAACTGGC CAAGGTGAGC AAGCCCGCTG CTTTCCGACA CGCCATGACG 
GATATCGAAA AGTACATCGA CGACCCCGCC GAGGTGGACG AGCAGGTGAA CAAAAGCTGC 
GGTGCGCCGA ATCACGCAAC CGAGGCTGAT TTTGACAAGA TCCGGGATGG CCTGGACAGC 
GAGGAGATTG TGGGAGAGGA AGCAACTGCC GATGGCAACA ATGGAGTGGT GGCCCATGTG 
GTGGGCAGCC CCGATGAGGG CGATGGCTTG GATGTGGAAA TCAATGCAGA TTCATCAGCG 
TCACCAGTCA CGTCGCCGGC GGTCACAACG AAGGCAGCTG GCAAGCGGAC GCCGAAAGCA 
AAGTCCGTGG CCGCTACATC GGTCAAGTCG ACAAAGGGAT CGGCAAAATC AGCACAGAAA 
CGGCGTACCT CTGCCCAACA GTCGCCCAGT GGGCCATCGA ATGCCAAGCG AGGCAAGCGT 
GATGTTTCCG GGGAGGCATT GCAGGACGCG GATGAAGCCA GCTCCACGCC CACTGGCAGA 
CGTCGGGTCG AGACTGACGC ACTCTTGGCC TCAATCGCTG CCAAGCGTGC ACCGAATGCA 
ATTGCTCTGC TTGACCGACC GGTTGTCACG CGGCCAGAGG CCCAAGTCAT CGACATGAGC 
TCGCGCTCCA ACACACTGGC CGATCGCGAT ATCGTGCCTT CAGAGCAGAC CTTCGGCTTC 
CTGGGTCTTG GAATGATGGG ATCCACCATA GTCAAGGATT TGATCTACAC GGGTCACAAG 
GTTGTGGTCT GGAACCGCAC CATTGACAAG TGCCAACCGT TTGCCGAAGC CGGCGCTGAG 
GTTAAGGACA CTCCCATGGA TGTTGTGGAA GCTGCCGACG TTATCTTTTG CTGTGTGTCC 
GATCCAAAGG GCGCCAAGGA TCTCGTATTT GGCAACTGCG GCGTTCTGCA GTTGAAGGAC 
TTGAACAATA AGGCCTACGT TGAAATGTCT ACAATTGATC CGGACACATC GTTGGACATT 
GGCGAGGGCA TCAAGCAGTG CAACGGTCGC TATCTTGAGG CACAAATTCA CGGCTCTCGG 
CAGGAGGCTG CCGAAGGCAT GCTCATCATT CTCGCCGGTG GAGATCGTTC AGTGTTCGAG 
GAGTGCCACT CGTGCTTCAA GACCATTGCC AAGAACACCT TCTTCTTGGG GAATATCGGT 
AACGCCTGCA AAGTAAACCT AATTTTGCAA ACCATCTTGG GAGTGAGTCT GGTCGGGTTG 
GCTGAGGCGT TAGCACTTGC TGATCGGTTC TCGATCTCTC TGAACGACAT CATAGACATT 
TTCGATTTGA CTTCAATGAA ATCACCCATG CTGCTGGCTA AGGGCAAAGA AATGGCCAAG 
GGTGACTTCA ATCCCCAGCA GCCTTTGAGC CACATGCAAC GCGACTTGCG CTTGGTGTTA 
AACATGGCTG AGAACCTGGA CCAGTCCATG CCCGTCACCA GCATCACCAA CGAGGTGTTC 
AAGCACACCA AGCGCTTGGG CTACAGTGAA CACGACTCGA GCGCCGTATT CGTAAGATCC 
AGATTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001285791.1
CDS	216..2024
Translation	MSKNKKLSLSGGDTTEKFIYKPKDLIWAKMKGFTPWPGMIVDPPLDLLSQQRRANTKCVFFFGSRNFAWIEENNIKPFEGPWKEELAKVSKPAAFRHAMTDIEKYIDDPAEVDEQVNKSCGAPNHATEADFDKIRDGLDSEEIVGEEATADGNNGVVAHVVGSPDEGDGLDVEINADSSASPVTSPAVTTKAAGKRTPKAKSVAATSVKSTKGSAKSAQKRRTSAQQSPSGPSNAKRGKRDVSGEALQDADEASSTPTGRRRVETDALLASIAAKRAPNAIALLDRPVVTRPEAQVIDMSSRSNTLADRDIVPSEQTFGFLGLGMMGSTIVKDLIYTGHKVVVWNRTIDKCQPFAEAGAEVKDTPMDVVEAADVIFCCVSDPKGAKDLVFGNCGVLQLKDLNNKAYVEMSTIDPDTSLDIGEGIKQCNGRYLEAQIHGSRQEAAEGMLIILAGGDRSVFEECHSCFKTIAKNTFFLGNIGNACKVNLILQTILGVSLVGLAEALALADRFSISLNDIIDIFDLTSMKSPMLLAKGKEMAKGDFNPQQPLSHMQRDLRLVLNMAENLDQSMPVTSITNEVFKHTKRLGYSEHDSSAVFVRSRF

Target ORF information:

RefSeq Version	NM_001298862.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Nucleosome-destabilizing factor (Ndf), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001298862.1

ORF Insert Sequence:

ATGTCGAAGA ATAAAAAACT GTCGCTGTCG GGCGGCGATA CGACGGAGAA ATTTATTTAC 
AAACCCAAGG ATCTGATATG GGCCAAAATG AAGGGCTTCA CTCCGTGGCC AGGAATGATT 
GTCGATCCTC CACTTGATCT GCTCAGCCAG CAGCGTCGGG CGAATACCAA ATGCGTGTTT 
TTCTTTGGAT CTAGGAATTT TGCCTGGATT GAGGAGAACA ACATCAAGCC CTTCGAAGGA 
CCGTGGAAGG AGGAACTGGC CAAGGTGAGC AAGCCCGCTG CTTTCCGACA CGCCATGACG 
GATATCGAAA AGTACATCGA CGACCCCGCC GAGGTGGACG AGCAGGTGAA CAAAAGCTGC 
GGTGCGCCGA ATCACGCAAC CGAGGCTGAT TTTGACAAGA TCCGGGATGG CCTGGACAGC 
GAGGAGATTG TGGGAGAGGA AGCAACTGCC GATGGCAACA ATGGAGTGGT GGCCCATGTG 
GTGGGCAGCC CCGATGAGGG CGATGGCTTG GATGTGGAAA TCAATGCAGA TTCATCAGCG 
TCACCAGTCA CGTCGCCGGC GGTCACAACG AAGGCAGCTG GCAAGCGGAC GCCGAAAGCA 
AAGTCCGTGG CCGCTACATC GGTCAAGTCG ACAAAGGGAT CGGCAAAATC AGCACAGAAA 
CGGCGTACCT CTGCCCAACA GTCGCCCAGT GGGCCATCGA ATGCCAAGCG AGGCAAGCGT 
GATGTTTCCG GGGAGGCATT GCAGGACGCG GATGAAGCCA GCTCCACGCC CACTGGCAGA 
CGTCGGGTCG AGACTGACGC ACTCTTGGCC TCAATCGCTG CCAAGCGTGC ACCGAATGCA 
ATTGCTCTGC TTGACCGACC GGTTGTCACG CGGCCAGAGG CCCAAGTCAT CGACATGAGC 
TCGCGCTCCA ACACACTGGC CGATCGCGAT ATCGTGCCTT CAGAGCAGAC CTTCGGCTTC 
CTGGGTCTTG GAATGATGGG ATCCACCATA GTCAAGGATT TGATCTACAC GGGTCACAAG 
GTTGTGGTCT GGAACCGCAC CATTGACAAG TGCCAACCGT TTGCCGAAGC CGGCGCTGAG 
GTTAAGGACA CTCCCATGGA TGTTGTGGAA GCTGCCGACG TTATCTTTTG CTGTGTGTCC 
GATCCAAAGG GCGCCAAGGA TCTCGTATTT GGCAACTGCG GCGTTCTGCA GTTGAAGGAC 
TTGAACAATA AGGCCTACGT TGAAATGTCT ACAATTGATC CGGACACATC GTTGGACATT 
GGCGAGGGCA TCAAGCAGTG CAACGGTCGC TATCTTGAGG CACAAATTCA CGGCTCTCGG 
CAGGAGGCTG CCGAAGGCAT GCTCATCATT CTCGCCGGTG GAGATCGTTC AGTGTTCGAG 
GAGTGCCACT CGTGCTTCAA GACCATTGCC AAGAACACCT TCTTCTTGGG GAATATCGGT 
AACGCCTGCA AAGTAAACCT AATTTTGCAA ACCATCTTGG GAGTGAGTCT GGTCGGGTTG 
GCTGAGGCGT TAGCACTTGC TGATCGGTTC TCGATCTCTC TGAACGACAT CATAGACATT 
TTCGATTTGA CTTCAATGAA ATCACCCATG CTGCTGGCTA AGGGCAAAGA AATGGCCAAG 
GGTGACTTCA ATCCCCAGCA GCCTTTGAGC CACATGCAAC GCGACTTGCG CTTGGTGTTA 
AACATGGCTG AGAACCTGGA CCAGTCCATG CCCGTCACCA GCATCACCAA CGAGGTGTTC 
AAGCACACCA AGCGCTTGGG CTACAGTGAA CACGACTCGA GCGCCGTATT CGTAAGATCC 
AGATTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ndf ( NM_135492.5 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_609336.3 Drosophila melanogaster Nucleosome-destabilizing factor (Ndf), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa15377
Clone ID Related Accession (Same CDS sequence)	NM_001298862.1 , NM_135492.5
Accession Version	NM_135492.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1809bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Nucleosome-destabilizing factor, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_135492.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGAAGA ATAAAAAACT GTCGCTGTCG GGCGGCGATA CGACGGAGAA ATTTATTTAC 
AAACCCAAGG ATCTGATATG GGCCAAAATG AAGGGCTTCA CTCCGTGGCC AGGAATGATT 
GTCGATCCTC CACTTGATCT GCTCAGCCAG CAGCGTCGGG CGAATACCAA ATGCGTGTTT 
TTCTTTGGAT CTAGGAATTT TGCCTGGATT GAGGAGAACA ACATCAAGCC CTTCGAAGGA 
CCGTGGAAGG AGGAACTGGC CAAGGTGAGC AAGCCCGCTG CTTTCCGACA CGCCATGACG 
GATATCGAAA AGTACATCGA CGACCCCGCC GAGGTGGACG AGCAGGTGAA CAAAAGCTGC 
GGTGCGCCGA ATCACGCAAC CGAGGCTGAT TTTGACAAGA TCCGGGATGG CCTGGACAGC 
GAGGAGATTG TGGGAGAGGA AGCAACTGCC GATGGCAACA ATGGAGTGGT GGCCCATGTG 
GTGGGCAGCC CCGATGAGGG CGATGGCTTG GATGTGGAAA TCAATGCAGA TTCATCAGCG 
TCACCAGTCA CGTCGCCGGC GGTCACAACG AAGGCAGCTG GCAAGCGGAC GCCGAAAGCA 
AAGTCCGTGG CCGCTACATC GGTCAAGTCG ACAAAGGGAT CGGCAAAATC AGCACAGAAA 
CGGCGTACCT CTGCCCAACA GTCGCCCAGT GGGCCATCGA ATGCCAAGCG AGGCAAGCGT 
GATGTTTCCG GGGAGGCATT GCAGGACGCG GATGAAGCCA GCTCCACGCC CACTGGCAGA 
CGTCGGGTCG AGACTGACGC ACTCTTGGCC TCAATCGCTG CCAAGCGTGC ACCGAATGCA 
ATTGCTCTGC TTGACCGACC GGTTGTCACG CGGCCAGAGG CCCAAGTCAT CGACATGAGC 
TCGCGCTCCA ACACACTGGC CGATCGCGAT ATCGTGCCTT CAGAGCAGAC CTTCGGCTTC 
CTGGGTCTTG GAATGATGGG ATCCACCATA GTCAAGGATT TGATCTACAC GGGTCACAAG 
GTTGTGGTCT GGAACCGCAC CATTGACAAG TGCCAACCGT TTGCCGAAGC CGGCGCTGAG 
GTTAAGGACA CTCCCATGGA TGTTGTGGAA GCTGCCGACG TTATCTTTTG CTGTGTGTCC 
GATCCAAAGG GCGCCAAGGA TCTCGTATTT GGCAACTGCG GCGTTCTGCA GTTGAAGGAC 
TTGAACAATA AGGCCTACGT TGAAATGTCT ACAATTGATC CGGACACATC GTTGGACATT 
GGCGAGGGCA TCAAGCAGTG CAACGGTCGC TATCTTGAGG CACAAATTCA CGGCTCTCGG 
CAGGAGGCTG CCGAAGGCAT GCTCATCATT CTCGCCGGTG GAGATCGTTC AGTGTTCGAG 
GAGTGCCACT CGTGCTTCAA GACCATTGCC AAGAACACCT TCTTCTTGGG GAATATCGGT 
AACGCCTGCA AAGTAAACCT AATTTTGCAA ACCATCTTGG GAGTGAGTCT GGTCGGGTTG 
GCTGAGGCGT TAGCACTTGC TGATCGGTTC TCGATCTCTC TGAACGACAT CATAGACATT 
TTCGATTTGA CTTCAATGAA ATCACCCATG CTGCTGGCTA AGGGCAAAGA AATGGCCAAG 
GGTGACTTCA ATCCCCAGCA GCCTTTGAGC CACATGCAAC GCGACTTGCG CTTGGTGTTA 
AACATGGCTG AGAACCTGGA CCAGTCCATG CCCGTCACCA GCATCACCAA CGAGGTGTTC 
AAGCACACCA AGCGCTTGGG CTACAGTGAA CACGACTCGA GCGCCGTATT CGTAAGATCC 
AGATTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_609336.3
CDS	216..2024
Translation	MSKNKKLSLSGGDTTEKFIYKPKDLIWAKMKGFTPWPGMIVDPPLDLLSQQRRANTKCVFFFGSRNFAWIEENNIKPFEGPWKEELAKVSKPAAFRHAMTDIEKYIDDPAEVDEQVNKSCGAPNHATEADFDKIRDGLDSEEIVGEEATADGNNGVVAHVVGSPDEGDGLDVEINADSSASPVTSPAVTTKAAGKRTPKAKSVAATSVKSTKGSAKSAQKRRTSAQQSPSGPSNAKRGKRDVSGEALQDADEASSTPTGRRRVETDALLASIAAKRAPNAIALLDRPVVTRPEAQVIDMSSRSNTLADRDIVPSEQTFGFLGLGMMGSTIVKDLIYTGHKVVVWNRTIDKCQPFAEAGAEVKDTPMDVVEAADVIFCCVSDPKGAKDLVFGNCGVLQLKDLNNKAYVEMSTIDPDTSLDIGEGIKQCNGRYLEAQIHGSRQEAAEGMLIILAGGDRSVFEECHSCFKTIAKNTFFLGNIGNACKVNLILQTILGVSLVGLAEALALADRFSISLNDIIDIFDLTSMKSPMLLAKGKEMAKGDFNPQQPLSHMQRDLRLVLNMAENLDQSMPVTSITNEVFKHTKRLGYSEHDSSAVFVRSRF

Target ORF information:

RefSeq Version	NM_135492.5
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Nucleosome-destabilizing factor (Ndf), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_135492.5

ORF Insert Sequence:

ATGTCGAAGA ATAAAAAACT GTCGCTGTCG GGCGGCGATA CGACGGAGAA ATTTATTTAC 
AAACCCAAGG ATCTGATATG GGCCAAAATG AAGGGCTTCA CTCCGTGGCC AGGAATGATT 
GTCGATCCTC CACTTGATCT GCTCAGCCAG CAGCGTCGGG CGAATACCAA ATGCGTGTTT 
TTCTTTGGAT CTAGGAATTT TGCCTGGATT GAGGAGAACA ACATCAAGCC CTTCGAAGGA 
CCGTGGAAGG AGGAACTGGC CAAGGTGAGC AAGCCCGCTG CTTTCCGACA CGCCATGACG 
GATATCGAAA AGTACATCGA CGACCCCGCC GAGGTGGACG AGCAGGTGAA CAAAAGCTGC 
GGTGCGCCGA ATCACGCAAC CGAGGCTGAT TTTGACAAGA TCCGGGATGG CCTGGACAGC 
GAGGAGATTG TGGGAGAGGA AGCAACTGCC GATGGCAACA ATGGAGTGGT GGCCCATGTG 
GTGGGCAGCC CCGATGAGGG CGATGGCTTG GATGTGGAAA TCAATGCAGA TTCATCAGCG 
TCACCAGTCA CGTCGCCGGC GGTCACAACG AAGGCAGCTG GCAAGCGGAC GCCGAAAGCA 
AAGTCCGTGG CCGCTACATC GGTCAAGTCG ACAAAGGGAT CGGCAAAATC AGCACAGAAA 
CGGCGTACCT CTGCCCAACA GTCGCCCAGT GGGCCATCGA ATGCCAAGCG AGGCAAGCGT 
GATGTTTCCG GGGAGGCATT GCAGGACGCG GATGAAGCCA GCTCCACGCC CACTGGCAGA 
CGTCGGGTCG AGACTGACGC ACTCTTGGCC TCAATCGCTG CCAAGCGTGC ACCGAATGCA 
ATTGCTCTGC TTGACCGACC GGTTGTCACG CGGCCAGAGG CCCAAGTCAT CGACATGAGC 
TCGCGCTCCA ACACACTGGC CGATCGCGAT ATCGTGCCTT CAGAGCAGAC CTTCGGCTTC 
CTGGGTCTTG GAATGATGGG ATCCACCATA GTCAAGGATT TGATCTACAC GGGTCACAAG 
GTTGTGGTCT GGAACCGCAC CATTGACAAG TGCCAACCGT TTGCCGAAGC CGGCGCTGAG 
GTTAAGGACA CTCCCATGGA TGTTGTGGAA GCTGCCGACG TTATCTTTTG CTGTGTGTCC 
GATCCAAAGG GCGCCAAGGA TCTCGTATTT GGCAACTGCG GCGTTCTGCA GTTGAAGGAC 
TTGAACAATA AGGCCTACGT TGAAATGTCT ACAATTGATC CGGACACATC GTTGGACATT 
GGCGAGGGCA TCAAGCAGTG CAACGGTCGC TATCTTGAGG CACAAATTCA CGGCTCTCGG 
CAGGAGGCTG CCGAAGGCAT GCTCATCATT CTCGCCGGTG GAGATCGTTC AGTGTTCGAG 
GAGTGCCACT CGTGCTTCAA GACCATTGCC AAGAACACCT TCTTCTTGGG GAATATCGGT 
AACGCCTGCA AAGTAAACCT AATTTTGCAA ACCATCTTGG GAGTGAGTCT GGTCGGGTTG 
GCTGAGGCGT TAGCACTTGC TGATCGGTTC TCGATCTCTC TGAACGACAT CATAGACATT 
TTCGATTTGA CTTCAATGAA ATCACCCATG CTGCTGGCTA AGGGCAAAGA AATGGCCAAG 
GGTGACTTCA ATCCCCAGCA GCCTTTGAGC CACATGCAAC GCGACTTGCG CTTGGTGTTA 
AACATGGCTG AGAACCTGGA CCAGTCCATG CCCGTCACCA GCATCACCAA CGAGGTGTTC 
AAGCACACCA AGCGCTTGGG CTACAGTGAA CACGACTCGA GCGCCGTATT CGTAAGATCC 
AGATTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ndf ( NM_001201838.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001188767.1 Drosophila melanogaster Nucleosome-destabilizing factor (Ndf), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa15788
Clone ID Related Accession (Same CDS sequence)	NM_001201838.2
Accession Version	NM_001201838.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1812bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Nucleosome-destabilizing factor, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_001201838.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGAAGA ATAAAAAACT GTCGCTGTCG GGCGGCGATA CGACGGAGAA ATTTATTTAC 
AAACCCAAGG ATCTGATATG GGCCAAAATG AAGGGCTTCA CTCCGTGGCC AGGAATGATT 
GTCGATCCTC CACTTGATCT GCTCAGCCAG CAGCGTCGGG CGAATACCAA ATGCGTGTTT 
TTCTTTGGAT CTAGGAATTT TGCCTGGATT GAGGAGAACA ACATCAAGCC CTTCGAAGGA 
CCGTGGAAGG AGGAACTGGC CAAGGTGAGC AAGCCCGCTG CTTTCCGACA CGCCATGACG 
GATATCGAAA AGTACATCGA CGACCCCGCC GAGGTGGACG AGCAGGTGAA CAAAAGCTGC 
GGTGCGCCGA ATCACGCAAC CGAGGCTGAT TTTGACAAGA TCCGGGATGG CCTGGACAGC 
GAGGAGATTG TGGGAGAGGA AGCAACTGCC GATGGCAACA ATGGAGTGGT GGCCCATGTG 
GTGGGCAGCC CCGATGAGGG CGATGGCTTG GATGTGGAAA TCAATGCAGA TTCATCAGCG 
TCACCAGTCA CGTCGCCGGC GGTCACAACG AAGGCAGCTG GCAAGCGGAC GCCGAAAGCA 
AAGTCCGTGG CCGCTACATC GGTCAAGTCG ACAAAGGGAT CGGCAAAATC AGCACAGAAA 
CGGCGTACCT CTGCCCAACA GTCGCCCAGT GGGCCATCGA ATGCCAAGCG AGGCAAGCGT 
GATGTTTCCG GGGAGGCATT GCAGGACGCG GATGAAGCCA GCTCCACGCC CACTGGCAGA 
CGTCGGGTCG AGACTGACGC ACTCTTGGCC TCAATCGCTG CCAAGCGTGC ACCGAATGCA 
ATTGCTCTGC TTGACCGACC GGTTGTCACG CGGCCAGAGG CCCAAGTCAT CGACATGAGC 
TCGCGCTCCA ACACACTGGC CGATCGCGAT ATCGTGCCTT CAGAGCAGAC CTTCGGCTTC 
CTGGGTCTTG GAATGATGGG ATCCACCATA GTCAAGGATT TGATCTACAC GGGTCACAAG 
GTTGTGGTCT GGAACCGCAC CATTGACAAG TGCCAACCGT TTGCCGAAGC CGGCGCTGAG 
GTTAAGGACA CTCCCATGGA TGTTGTGGAA GCTGCCGACG TTATCTTTTG CTGTGTGTCC 
GATCCAAAGG GCGCCAAGGA TCTCGTATTT GGCAACTGCG GCGTTCTGCA GTTGAAGGAC 
TTGAACAATA AGGCCTACGT TGAAATGTCT ACAATTGATC CGGACACATC GTTGGACATT 
GGCGAGGGCA TCAAGCAGTG CAACGGTCGC TATCTTGAGG CACAAATTCA CGGCTCTCGG 
CAGGAGGCTG CCGAAGGCAT GCTCATCATT CTCGCCGGTG GAGATCGTTC AGTGTTCGAG 
GAGTGCCACT CGTGCTTCAA GACCATTGCC AAGAACACCT TCTTCTTGGG GACAGATATC 
GGTAACGCCT GCAAAGTAAA CCTAATTTTG CAAACCATCT TGGGAGTGAG TCTGGTCGGG 
TTGGCTGAGG CGTTAGCACT TGCTGATCGG TTCTCGATCT CTCTGAACGA CATCATAGAC 
ATTTTCGATT TGACTTCAAT GAAATCACCC ATGCTGCTGG CTAAGGGCAA AGAAATGGCC 
AAGGGTGACT TCAATCCCCA GCAGCCTTTG AGCCACATGC AACGCGACTT GCGCTTGGTG 
TTAAACATGG CTGAGAACCT GGACCAGTCC ATGCCCGTCA CCAGCATCAC CAACGAGGTG 
TTCAAGCACA CCAAGCGCTT GGGCTACAGT GAACACGACT CGAGCGCCGT ATTCGTAAGA 
TCCAGATTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001188767.1
CDS	216..2027
Translation	MSKNKKLSLSGGDTTEKFIYKPKDLIWAKMKGFTPWPGMIVDPPLDLLSQQRRANTKCVFFFGSRNFAWIEENNIKPFEGPWKEELAKVSKPAAFRHAMTDIEKYIDDPAEVDEQVNKSCGAPNHATEADFDKIRDGLDSEEIVGEEATADGNNGVVAHVVGSPDEGDGLDVEINADSSASPVTSPAVTTKAAGKRTPKAKSVAATSVKSTKGSAKSAQKRRTSAQQSPSGPSNAKRGKRDVSGEALQDADEASSTPTGRRRVETDALLASIAAKRAPNAIALLDRPVVTRPEAQVIDMSSRSNTLADRDIVPSEQTFGFLGLGMMGSTIVKDLIYTGHKVVVWNRTIDKCQPFAEAGAEVKDTPMDVVEAADVIFCCVSDPKGAKDLVFGNCGVLQLKDLNNKAYVEMSTIDPDTSLDIGEGIKQCNGRYLEAQIHGSRQEAAEGMLIILAGGDRSVFEECHSCFKTIAKNTFFLGTDIGNACKVNLILQTILGVSLVGLAEALALADRFSISLNDIIDIFDLTSMKSPMLLAKGKEMAKGDFNPQQPLSHMQRDLRLVLNMAENLDQSMPVTSITNEVFKHTKRLGYSEHDSSAVFVRSRF

Target ORF information:

RefSeq Version	NM_001201838.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Nucleosome-destabilizing factor (Ndf), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001201838.2

ORF Insert Sequence:

ATGTCGAAGA ATAAAAAACT GTCGCTGTCG GGCGGCGATA CGACGGAGAA ATTTATTTAC 
AAACCCAAGG ATCTGATATG GGCCAAAATG AAGGGCTTCA CTCCGTGGCC AGGAATGATT 
GTCGATCCTC CACTTGATCT GCTCAGCCAG CAGCGTCGGG CGAATACCAA ATGCGTGTTT 
TTCTTTGGAT CTAGGAATTT TGCCTGGATT GAGGAGAACA ACATCAAGCC CTTCGAAGGA 
CCGTGGAAGG AGGAACTGGC CAAGGTGAGC AAGCCCGCTG CTTTCCGACA CGCCATGACG 
GATATCGAAA AGTACATCGA CGACCCCGCC GAGGTGGACG AGCAGGTGAA CAAAAGCTGC 
GGTGCGCCGA ATCACGCAAC CGAGGCTGAT TTTGACAAGA TCCGGGATGG CCTGGACAGC 
GAGGAGATTG TGGGAGAGGA AGCAACTGCC GATGGCAACA ATGGAGTGGT GGCCCATGTG 
GTGGGCAGCC CCGATGAGGG CGATGGCTTG GATGTGGAAA TCAATGCAGA TTCATCAGCG 
TCACCAGTCA CGTCGCCGGC GGTCACAACG AAGGCAGCTG GCAAGCGGAC GCCGAAAGCA 
AAGTCCGTGG CCGCTACATC GGTCAAGTCG ACAAAGGGAT CGGCAAAATC AGCACAGAAA 
CGGCGTACCT CTGCCCAACA GTCGCCCAGT GGGCCATCGA ATGCCAAGCG AGGCAAGCGT 
GATGTTTCCG GGGAGGCATT GCAGGACGCG GATGAAGCCA GCTCCACGCC CACTGGCAGA 
CGTCGGGTCG AGACTGACGC ACTCTTGGCC TCAATCGCTG CCAAGCGTGC ACCGAATGCA 
ATTGCTCTGC TTGACCGACC GGTTGTCACG CGGCCAGAGG CCCAAGTCAT CGACATGAGC 
TCGCGCTCCA ACACACTGGC CGATCGCGAT ATCGTGCCTT CAGAGCAGAC CTTCGGCTTC 
CTGGGTCTTG GAATGATGGG ATCCACCATA GTCAAGGATT TGATCTACAC GGGTCACAAG 
GTTGTGGTCT GGAACCGCAC CATTGACAAG TGCCAACCGT TTGCCGAAGC CGGCGCTGAG 
GTTAAGGACA CTCCCATGGA TGTTGTGGAA GCTGCCGACG TTATCTTTTG CTGTGTGTCC 
GATCCAAAGG GCGCCAAGGA TCTCGTATTT GGCAACTGCG GCGTTCTGCA GTTGAAGGAC 
TTGAACAATA AGGCCTACGT TGAAATGTCT ACAATTGATC CGGACACATC GTTGGACATT 
GGCGAGGGCA TCAAGCAGTG CAACGGTCGC TATCTTGAGG CACAAATTCA CGGCTCTCGG 
CAGGAGGCTG CCGAAGGCAT GCTCATCATT CTCGCCGGTG GAGATCGTTC AGTGTTCGAG 
GAGTGCCACT CGTGCTTCAA GACCATTGCC AAGAACACCT TCTTCTTGGG GACAGATATC 
GGTAACGCCT GCAAAGTAAA CCTAATTTTG CAAACCATCT TGGGAGTGAG TCTGGTCGGG 
TTGGCTGAGG CGTTAGCACT TGCTGATCGG TTCTCGATCT CTCTGAACGA CATCATAGAC 
ATTTTCGATT TGACTTCAAT GAAATCACCC ATGCTGCTGG CTAAGGGCAA AGAAATGGCC 
AAGGGTGACT TCAATCCCCA GCAGCCTTTG AGCCACATGC AACGCGACTT GCGCTTGGTG 
TTAAACATGG CTGAGAACCT GGACCAGTCC ATGCCCGTCA CCAGCATCAC CAACGAGGTG 
TTCAAGCACA CCAAGCGCTT GGGCTACAGT GAACACGACT CGAGCGCCGT ATTCGTAAGA 
TCCAGATTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Ndf cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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