koko cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	koko
Entrez Gene ID	14462485
Full Name	kokopelli
Synonyms	CG31232,CG44010,CG7683,Dmel\CG44010,Dmel_CG31232
General protein information	Preferred Names kokopelli Names CG44010 gene product from transcript CG44010-RE CG44010-PB CG44010-PC CG44010-PD CG44010-PE koko-PB koko-PC koko-PD koko-PE
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 91A6-91A6

mRNA and Protein(s)

mRNA	Protein	Name
NM_169811.3	NP_732338.1	kokopelli, isoform B
NM_169812.3	NP_732339.1	kokopelli, isoform C
NM_169813.3	NP_732340.1	kokopelli, isoform D
NM_169814.3	NP_732341.1	kokopelli, isoform E

Pathways from BioSystems

Homologies

Homo sapiens (human)	FAM58A	NP_689487.2
Mus musculus (house mouse)	Fam58b	NP_932106.1
Pan troglodytes (chimpanzee)	FAM58A	XP_001137614.2
	FAM58A	XP_001083983.1
Canis lupus familiaris (dog)	FAM58A	XP_549359.3
Bos taurus (cattle)	FAM58A	XP_002699785.1
Rattus norvegicus (Norway rat)	Fam58b	NP_001020583.1
Drosophila melanogaster (fruit fly)	koko	NP_732339.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012763	XP_307016.3
Xenopus tropicalis (tropical clawed frog)	fam58a	NP_001016916.1
Danio rerio (zebrafish)	fam58a	NP_001018459.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005056	XP_313929.4

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following koko gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the koko cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa08987	NM_169812.3 Latest version!	Drosophila melanogaster kokopelli (koko), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OFa08987	NM_169814.3 Latest version!	Drosophila melanogaster kokopelli (koko), transcript variant E, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OFa08987	NM_169813.3 Latest version!	Drosophila melanogaster kokopelli (koko), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OFa08987	NM_169811.3 Latest version!	Drosophila melanogaster kokopelli (koko), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

koko ( NM_169811.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_732338.1 Drosophila melanogaster kokopelli (koko), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa08987
Clone ID Related Accession (Same CDS sequence)	NM_169811.3 , NM_169812.3 , NM_169813.3 , NM_169814.3
Accession Version	NM_169811.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 780bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	kokopelli, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_169811.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAAAACG TTTTGGATGT CATGTCGATG CAGCAGCACG TGGAGTTGAA CAAGGCGCAG 
ACCATGAAGC CCATCGACTA TCGCAAAATG AACAAACCCG GCGTGGTGCC GATGTACATC 
TTTGAGTGCG CTGCCAAGTT GAAAATGAAA CCGCTGACGG CGGCCTGTGC GGCCATAGTG 
TTCCATCGCT TCTTCCGCGA GGTCAAGGCC AGCGACTACG ATGAGTTCCT CATTGCCGCG 
GGCTCGCTGT ACTTGGCTGG CAAAATTAAG GAAGACGAAA GTGTCAAGAT ACGCGATGTC 
ATCAACGTGG CATACTGCAC CCTTAACCGG GGCAATGATC CAGTCGACCT GAACGATGAG 
TACTGGTCGA TGCGGGATGC CATTGTCCAG GCTGAGCTGC TCATCACGCG CACCCTGTGC 
TTCGATCTCA ACATAGATCT GGCCCACAAG TACTTACTCC ACTACATGAA GACCCTACAA 
GACTGGGTGG GCACTGAGGT GTGGAACTCG GTGCCCATTG CCAAGGCAGC CGCCTCGTAT 
CTGCAAGACT TTCACCACAG CGCCAATATC CTCAAGTACA AGCCCACCCA TGTGGCCATT 
GGGTGCCTGT CGCTGGCCCT CCAGACCTAC GGCATCCAGG TGCCGCTAAC CGACGAGTCC 
GACGAATCCG CCATGTGGTA CAAGCCCTTG GTCAAGGACT TCACCCGAGA GAACCAGTGG 
GAGATCATCG AGAACGTGAT CGAGGTCTAC AAGAACGAGG GCTTCCTGAA TGCCACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_732338.1
CDS	219..998
Translation	MKNVLDVMSMQQHVELNKAQTMKPIDYRKMNKPGVVPMYIFECAAKLKMKPLTAACAAIVFHRFFREVKASDYDEFLIAAGSLYLAGKIKEDESVKIRDVINVAYCTLNRGNDPVDLNDEYWSMRDAIVQAELLITRTLCFDLNIDLAHKYLLHYMKTLQDWVGTEVWNSVPIAKAAASYLQDFHHSANILKYKPTHVAIGCLSLALQTYGIQVPLTDESDESAMWYKPLVKDFTRENQWEIIENVIEVYKNEGFLNAT

Target ORF information:

RefSeq Version	NM_169811.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster kokopelli (koko), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_169811.3

ORF Insert Sequence:

ATGAAAAACG TTTTGGATGT CATGTCGATG CAGCAGCACG TGGAGTTGAA CAAGGCGCAG 
ACCATGAAGC CCATCGACTA TCGCAAAATG AACAAACCCG GCGTGGTGCC GATGTACATC 
TTTGAGTGCG CTGCCAAGTT GAAAATGAAA CCGCTGACGG CGGCCTGTGC GGCCATAGTG 
TTCCATCGCT TCTTCCGCGA GGTCAAGGCC AGCGACTACG ATGAGTTCCT CATTGCCGCG 
GGCTCGCTGT ACTTGGCTGG CAAAATTAAG GAAGACGAAA GTGTCAAGAT ACGCGATGTC 
ATCAACGTGG CATACTGCAC CCTTAACCGG GGCAATGATC CAGTCGACCT GAACGATGAG 
TACTGGTCGA TGCGGGATGC CATTGTCCAG GCTGAGCTGC TCATCACGCG CACCCTGTGC 
TTCGATCTCA ACATAGATCT GGCCCACAAG TACTTACTCC ACTACATGAA GACCCTACAA 
GACTGGGTGG GCACTGAGGT GTGGAACTCG GTGCCCATTG CCAAGGCAGC CGCCTCGTAT 
CTGCAAGACT TTCACCACAG CGCCAATATC CTCAAGTACA AGCCCACCCA TGTGGCCATT 
GGGTGCCTGT CGCTGGCCCT CCAGACCTAC GGCATCCAGG TGCCGCTAAC CGACGAGTCC 
GACGAATCCG CCATGTGGTA CAAGCCCTTG GTCAAGGACT TCACCCGAGA GAACCAGTGG 
GAGATCATCG AGAACGTGAT CGAGGTCTAC AAGAACGAGG GCTTCCTGAA TGCCACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

koko ( NM_169812.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_732339.1 Drosophila melanogaster kokopelli (koko), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa08987
Clone ID Related Accession (Same CDS sequence)	NM_169811.3 , NM_169812.3 , NM_169813.3 , NM_169814.3
Accession Version	NM_169812.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 780bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	kokopelli, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_169812.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAAAACG TTTTGGATGT CATGTCGATG CAGCAGCACG TGGAGTTGAA CAAGGCGCAG 
ACCATGAAGC CCATCGACTA TCGCAAAATG AACAAACCCG GCGTGGTGCC GATGTACATC 
TTTGAGTGCG CTGCCAAGTT GAAAATGAAA CCGCTGACGG CGGCCTGTGC GGCCATAGTG 
TTCCATCGCT TCTTCCGCGA GGTCAAGGCC AGCGACTACG ATGAGTTCCT CATTGCCGCG 
GGCTCGCTGT ACTTGGCTGG CAAAATTAAG GAAGACGAAA GTGTCAAGAT ACGCGATGTC 
ATCAACGTGG CATACTGCAC CCTTAACCGG GGCAATGATC CAGTCGACCT GAACGATGAG 
TACTGGTCGA TGCGGGATGC CATTGTCCAG GCTGAGCTGC TCATCACGCG CACCCTGTGC 
TTCGATCTCA ACATAGATCT GGCCCACAAG TACTTACTCC ACTACATGAA GACCCTACAA 
GACTGGGTGG GCACTGAGGT GTGGAACTCG GTGCCCATTG CCAAGGCAGC CGCCTCGTAT 
CTGCAAGACT TTCACCACAG CGCCAATATC CTCAAGTACA AGCCCACCCA TGTGGCCATT 
GGGTGCCTGT CGCTGGCCCT CCAGACCTAC GGCATCCAGG TGCCGCTAAC CGACGAGTCC 
GACGAATCCG CCATGTGGTA CAAGCCCTTG GTCAAGGACT TCACCCGAGA GAACCAGTGG 
GAGATCATCG AGAACGTGAT CGAGGTCTAC AAGAACGAGG GCTTCCTGAA TGCCACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_732339.1
CDS	187..966
Translation	MKNVLDVMSMQQHVELNKAQTMKPIDYRKMNKPGVVPMYIFECAAKLKMKPLTAACAAIVFHRFFREVKASDYDEFLIAAGSLYLAGKIKEDESVKIRDVINVAYCTLNRGNDPVDLNDEYWSMRDAIVQAELLITRTLCFDLNIDLAHKYLLHYMKTLQDWVGTEVWNSVPIAKAAASYLQDFHHSANILKYKPTHVAIGCLSLALQTYGIQVPLTDESDESAMWYKPLVKDFTRENQWEIIENVIEVYKNEGFLNAT

Target ORF information:

RefSeq Version	NM_169812.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster kokopelli (koko), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_169812.3

ORF Insert Sequence:

ATGAAAAACG TTTTGGATGT CATGTCGATG CAGCAGCACG TGGAGTTGAA CAAGGCGCAG 
ACCATGAAGC CCATCGACTA TCGCAAAATG AACAAACCCG GCGTGGTGCC GATGTACATC 
TTTGAGTGCG CTGCCAAGTT GAAAATGAAA CCGCTGACGG CGGCCTGTGC GGCCATAGTG 
TTCCATCGCT TCTTCCGCGA GGTCAAGGCC AGCGACTACG ATGAGTTCCT CATTGCCGCG 
GGCTCGCTGT ACTTGGCTGG CAAAATTAAG GAAGACGAAA GTGTCAAGAT ACGCGATGTC 
ATCAACGTGG CATACTGCAC CCTTAACCGG GGCAATGATC CAGTCGACCT GAACGATGAG 
TACTGGTCGA TGCGGGATGC CATTGTCCAG GCTGAGCTGC TCATCACGCG CACCCTGTGC 
TTCGATCTCA ACATAGATCT GGCCCACAAG TACTTACTCC ACTACATGAA GACCCTACAA 
GACTGGGTGG GCACTGAGGT GTGGAACTCG GTGCCCATTG CCAAGGCAGC CGCCTCGTAT 
CTGCAAGACT TTCACCACAG CGCCAATATC CTCAAGTACA AGCCCACCCA TGTGGCCATT 
GGGTGCCTGT CGCTGGCCCT CCAGACCTAC GGCATCCAGG TGCCGCTAAC CGACGAGTCC 
GACGAATCCG CCATGTGGTA CAAGCCCTTG GTCAAGGACT TCACCCGAGA GAACCAGTGG 
GAGATCATCG AGAACGTGAT CGAGGTCTAC AAGAACGAGG GCTTCCTGAA TGCCACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

koko ( NM_169813.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_732340.1 Drosophila melanogaster kokopelli (koko), transcript variant D, mRNA.

Price & Availability↑

CloneID	OFa08987
Clone ID Related Accession (Same CDS sequence)	NM_169811.3 , NM_169812.3 , NM_169813.3 , NM_169814.3
Accession Version	NM_169813.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 780bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	kokopelli, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_169813.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAAAACG TTTTGGATGT CATGTCGATG CAGCAGCACG TGGAGTTGAA CAAGGCGCAG 
ACCATGAAGC CCATCGACTA TCGCAAAATG AACAAACCCG GCGTGGTGCC GATGTACATC 
TTTGAGTGCG CTGCCAAGTT GAAAATGAAA CCGCTGACGG CGGCCTGTGC GGCCATAGTG 
TTCCATCGCT TCTTCCGCGA GGTCAAGGCC AGCGACTACG ATGAGTTCCT CATTGCCGCG 
GGCTCGCTGT ACTTGGCTGG CAAAATTAAG GAAGACGAAA GTGTCAAGAT ACGCGATGTC 
ATCAACGTGG CATACTGCAC CCTTAACCGG GGCAATGATC CAGTCGACCT GAACGATGAG 
TACTGGTCGA TGCGGGATGC CATTGTCCAG GCTGAGCTGC TCATCACGCG CACCCTGTGC 
TTCGATCTCA ACATAGATCT GGCCCACAAG TACTTACTCC ACTACATGAA GACCCTACAA 
GACTGGGTGG GCACTGAGGT GTGGAACTCG GTGCCCATTG CCAAGGCAGC CGCCTCGTAT 
CTGCAAGACT TTCACCACAG CGCCAATATC CTCAAGTACA AGCCCACCCA TGTGGCCATT 
GGGTGCCTGT CGCTGGCCCT CCAGACCTAC GGCATCCAGG TGCCGCTAAC CGACGAGTCC 
GACGAATCCG CCATGTGGTA CAAGCCCTTG GTCAAGGACT TCACCCGAGA GAACCAGTGG 
GAGATCATCG AGAACGTGAT CGAGGTCTAC AAGAACGAGG GCTTCCTGAA TGCCACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_732340.1
CDS	117..896
Translation	MKNVLDVMSMQQHVELNKAQTMKPIDYRKMNKPGVVPMYIFECAAKLKMKPLTAACAAIVFHRFFREVKASDYDEFLIAAGSLYLAGKIKEDESVKIRDVINVAYCTLNRGNDPVDLNDEYWSMRDAIVQAELLITRTLCFDLNIDLAHKYLLHYMKTLQDWVGTEVWNSVPIAKAAASYLQDFHHSANILKYKPTHVAIGCLSLALQTYGIQVPLTDESDESAMWYKPLVKDFTRENQWEIIENVIEVYKNEGFLNAT

Target ORF information:

RefSeq Version	NM_169813.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster kokopelli (koko), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_169813.3

ORF Insert Sequence:

ATGAAAAACG TTTTGGATGT CATGTCGATG CAGCAGCACG TGGAGTTGAA CAAGGCGCAG 
ACCATGAAGC CCATCGACTA TCGCAAAATG AACAAACCCG GCGTGGTGCC GATGTACATC 
TTTGAGTGCG CTGCCAAGTT GAAAATGAAA CCGCTGACGG CGGCCTGTGC GGCCATAGTG 
TTCCATCGCT TCTTCCGCGA GGTCAAGGCC AGCGACTACG ATGAGTTCCT CATTGCCGCG 
GGCTCGCTGT ACTTGGCTGG CAAAATTAAG GAAGACGAAA GTGTCAAGAT ACGCGATGTC 
ATCAACGTGG CATACTGCAC CCTTAACCGG GGCAATGATC CAGTCGACCT GAACGATGAG 
TACTGGTCGA TGCGGGATGC CATTGTCCAG GCTGAGCTGC TCATCACGCG CACCCTGTGC 
TTCGATCTCA ACATAGATCT GGCCCACAAG TACTTACTCC ACTACATGAA GACCCTACAA 
GACTGGGTGG GCACTGAGGT GTGGAACTCG GTGCCCATTG CCAAGGCAGC CGCCTCGTAT 
CTGCAAGACT TTCACCACAG CGCCAATATC CTCAAGTACA AGCCCACCCA TGTGGCCATT 
GGGTGCCTGT CGCTGGCCCT CCAGACCTAC GGCATCCAGG TGCCGCTAAC CGACGAGTCC 
GACGAATCCG CCATGTGGTA CAAGCCCTTG GTCAAGGACT TCACCCGAGA GAACCAGTGG 
GAGATCATCG AGAACGTGAT CGAGGTCTAC AAGAACGAGG GCTTCCTGAA TGCCACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

koko ( NM_169814.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_732341.1 Drosophila melanogaster kokopelli (koko), transcript variant E, mRNA.

Price & Availability↑

CloneID	OFa08987
Clone ID Related Accession (Same CDS sequence)	NM_169811.3 , NM_169812.3 , NM_169813.3 , NM_169814.3
Accession Version	NM_169814.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 780bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	kokopelli, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_169814.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAAAACG TTTTGGATGT CATGTCGATG CAGCAGCACG TGGAGTTGAA CAAGGCGCAG 
ACCATGAAGC CCATCGACTA TCGCAAAATG AACAAACCCG GCGTGGTGCC GATGTACATC 
TTTGAGTGCG CTGCCAAGTT GAAAATGAAA CCGCTGACGG CGGCCTGTGC GGCCATAGTG 
TTCCATCGCT TCTTCCGCGA GGTCAAGGCC AGCGACTACG ATGAGTTCCT CATTGCCGCG 
GGCTCGCTGT ACTTGGCTGG CAAAATTAAG GAAGACGAAA GTGTCAAGAT ACGCGATGTC 
ATCAACGTGG CATACTGCAC CCTTAACCGG GGCAATGATC CAGTCGACCT GAACGATGAG 
TACTGGTCGA TGCGGGATGC CATTGTCCAG GCTGAGCTGC TCATCACGCG CACCCTGTGC 
TTCGATCTCA ACATAGATCT GGCCCACAAG TACTTACTCC ACTACATGAA GACCCTACAA 
GACTGGGTGG GCACTGAGGT GTGGAACTCG GTGCCCATTG CCAAGGCAGC CGCCTCGTAT 
CTGCAAGACT TTCACCACAG CGCCAATATC CTCAAGTACA AGCCCACCCA TGTGGCCATT 
GGGTGCCTGT CGCTGGCCCT CCAGACCTAC GGCATCCAGG TGCCGCTAAC CGACGAGTCC 
GACGAATCCG CCATGTGGTA CAAGCCCTTG GTCAAGGACT TCACCCGAGA GAACCAGTGG 
GAGATCATCG AGAACGTGAT CGAGGTCTAC AAGAACGAGG GCTTCCTGAA TGCCACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_732341.1
CDS	62..841
Translation	MKNVLDVMSMQQHVELNKAQTMKPIDYRKMNKPGVVPMYIFECAAKLKMKPLTAACAAIVFHRFFREVKASDYDEFLIAAGSLYLAGKIKEDESVKIRDVINVAYCTLNRGNDPVDLNDEYWSMRDAIVQAELLITRTLCFDLNIDLAHKYLLHYMKTLQDWVGTEVWNSVPIAKAAASYLQDFHHSANILKYKPTHVAIGCLSLALQTYGIQVPLTDESDESAMWYKPLVKDFTRENQWEIIENVIEVYKNEGFLNAT

Target ORF information:

RefSeq Version	NM_169814.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster kokopelli (koko), transcript variant E, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_169814.3

ORF Insert Sequence:

ATGAAAAACG TTTTGGATGT CATGTCGATG CAGCAGCACG TGGAGTTGAA CAAGGCGCAG 
ACCATGAAGC CCATCGACTA TCGCAAAATG AACAAACCCG GCGTGGTGCC GATGTACATC 
TTTGAGTGCG CTGCCAAGTT GAAAATGAAA CCGCTGACGG CGGCCTGTGC GGCCATAGTG 
TTCCATCGCT TCTTCCGCGA GGTCAAGGCC AGCGACTACG ATGAGTTCCT CATTGCCGCG 
GGCTCGCTGT ACTTGGCTGG CAAAATTAAG GAAGACGAAA GTGTCAAGAT ACGCGATGTC 
ATCAACGTGG CATACTGCAC CCTTAACCGG GGCAATGATC CAGTCGACCT GAACGATGAG 
TACTGGTCGA TGCGGGATGC CATTGTCCAG GCTGAGCTGC TCATCACGCG CACCCTGTGC 
TTCGATCTCA ACATAGATCT GGCCCACAAG TACTTACTCC ACTACATGAA GACCCTACAA 
GACTGGGTGG GCACTGAGGT GTGGAACTCG GTGCCCATTG CCAAGGCAGC CGCCTCGTAT 
CTGCAAGACT TTCACCACAG CGCCAATATC CTCAAGTACA AGCCCACCCA TGTGGCCATT 
GGGTGCCTGT CGCTGGCCCT CCAGACCTAC GGCATCCAGG TGCCGCTAAC CGACGAGTCC 
GACGAATCCG CCATGTGGTA CAAGCCCTTG GTCAAGGACT TCACCCGAGA GAACCAGTGG 
GAGATCATCG AGAACGTGAT CGAGGTCTAC AAGAACGAGG GCTTCCTGAA TGCCACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
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koko cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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