Tre1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Tre1
Entrez Gene ID	140439
Full Name	Trapped in endoderm 1
Synonyms	BEST:LD12308,CG3171,Dmel\CG3171,LD12308,Tre,Tre-1,anon-WO0170980.16,anon-WO0170980.17,anon-WO0170981.1,sctt,tre-1,tre1
General protein information	Preferred Names Trapped in endoderm 1 Names CG3171 gene product from transcript CG3171-RA CG3171-PA CG3171-PB CG3171-PC G protein coupled receptor Tre1-PA Tre1-PB Tre1-PC scattershot trapped in endoderm 1 trapped in endoderm-1
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 5A11-5A12

mRNA and Protein(s)

mRNA	Protein	Name
NM_080053.4	NP_524792.1	trapped in endoderm 1, isoform A
NM_001297972.1	NP_001284901.1	trapped in endoderm 1, isoform C
NM_001258605.1	NP_001245534.1	trapped in endoderm 1, isoform B

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Gpr84	NP_001102979.1
Bos taurus (cattle)	GPR84	NP_001033657.1
Homo sapiens (human)	GPR84	NP_065103.1
Canis lupus familiaris (dog)	GPR84	XP_003433542.1
Drosophila melanogaster (fruit fly)	Tre1	NP_001245534.1
Pan troglodytes (chimpanzee)	GPR84	XP_522412.1
	GPR84	XP_001109742.1
Mus musculus (house mouse)	Gpr84	NP_109645.1
Danio rerio (zebrafish)	gpr84	NP_001099167.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004930	XP_315017.4
Xenopus tropicalis (tropical clawed frog)	gpr84	NP_001072335.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Bifurcation of the Tre1 G protein-coupled receptor signaling response via G protein-dependent and independent branches enables distinct spatiotemporal regulation of germ cell migration.
Title: Domain-specific control of germ cell polarity and migration by multifunction Tre1 GPCR.

feminizing the Tre1-expressing neurons in male flies leads to rapid courtship initiation, a phenotype that leads to increased reproductive success; Tre1 is expressed in a sexually dimorphic pattern in olfactory sensory neurons and the central nervous system
Title: A Novel Gene Controlling the Timing of Courtship Initiation in Drosophila melanogaster.

role of the arginine of the NRY motif in Tre1 was investigated; results suggest that a salt bridge may form between this critical arginine and an aspartic acid in transmembrane alpha-helix (TM6)in Tre1
Title: Molecular dynamics simulations on the Tre1 G protein-coupled receptor: exploring the role of the arginine of the NRY motif in Tre1 structure.

The scattershot allele results in an in-frame deletion of 8 amino acids at the junction of the third transmembrane domain and the second intracellular loop of Tre1 that dramatically impairs the function of this GPCR in germ cell migration.
Title: An evolutionarily conserved arginine is essential for Tre1 G protein-coupled receptor function during germ cell migration in Drosophila melanogaster.

plays an important role in regulation of germ cell migration, polarity , cell adhesion, and cadherin binding. (review)
Title: [New insights into cell migration from Drosophila germ cell migration].

The following Tre1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Tre1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa02483	NM_001258605.1 Latest version!	Drosophila melanogaster trapped in endoderm 1, transcript variant B (Tre1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa02483	NM_080053.4 Latest version!	Drosophila melanogaster trapped in endoderm 1, transcript variant A (Tre1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa22292	NM_001297972.1 Latest version!	Drosophila melanogaster trapped in endoderm 1, transcript variant C (Tre1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Tre1 ( NM_080053.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_524792.1 Drosophila melanogaster trapped in endoderm 1, transcript variant A (Tre1), mRNA.

Price & Availability↑

CloneID	OFa02483
Clone ID Related Accession (Same CDS sequence)	NM_080053.4 , NM_001258605.1
Accession Version	NM_080053.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1179bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	trapped in endoderm 1, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_080053.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGATCAGG ACATGGGCAT GGCAACGGGC TACTTTCAGG ACGCAGACAT GCAGATGGAT 
GAACCGGCGG CCGCCACGCA ATCGATCTAC CCGCATTCGG CGACATTATT CGCGGCCATT 
AGTGCCTGTG TCTTTGTGAC GATCGGCGTT CTTGGCAACC TGATTACCCT GCTGGCGCTG 
CTCAAGAGCC CCACGATACG GGAGCATGCC ACAACCGCCT TCGTCATTTC GCTAAGCATC 
TCCGACCTGC TCTTCTGCTC CTTCAGCCTG CCACTGACCG CAGTGCGATT CTTCCAGGAG 
AGCTGGACTT TTGGTACCAC ATTGTGCAAG ATCTTTCCGG TGATCTTCTA TGGCAATGTG 
GCTGTTTCAC TCCTCAGCAT GGTGGGCATC ACCCTGAACA GATATATACT CATCGCTTGC 
CACAGCCGCT ACTCGCAGAT ATATAAGCCT AAGTTCATAA CCCTGCAGCT GTTGTTCGTT 
TGGGCCGTCT CCTTTCTGCT GTTGCTGCCG CCCATATTGG GCATCTGGGG CGAGATGGGC 
CTGGACGAGG CCACCTTCTC CTGCACAATA CTCAAGAAGG AGGGGCGATC GATCAAGAAG 
ACGCTGTTCG TGATCGGCTT CCTGCTGCCT TGCCTGGTGA TCATCGTTTC GTACTCGTGC 
ATCTACATTA CGGTGCTGCA CCAGAAAAAG AAGATTCGCA ACCACGATAA CTTCCAGATC 
GCAGCGGCCA AGGGCTCCTC GTCGTCCGGC GGCGGATCCT ACATGACCAC CACATGCACG 
CGCAAGGCGC GCGAAGACAA TCGACTGACC GTCATGATGG TCACCATCTT TCTGTGCTTC 
CTCGTCTGCT TCCTGCCGCT AATGCTGGCC AATGTGGTGG ACGACGAGCG CAATACCTCG 
TACCCCTGGC TGCACATCAT CGCCTCCGTG ATGGCCTGGG CTTCCAGTGT CATTAACCCG 
ATCATCTATG CGGCCAGCAA TCGCAACTAC AGAGTGGCCT ACTACAAGAT CTTTGCGCTG 
CTCAAGTTCT GGGGCGAACC GTTGTCGCCG ATGCCGAGTA GAAACTATCA CCAAAGCAAG 
AACTCCAAGG AGCTGTCGGG CGTCATCCGC AGCACGCCGC TGTTTCACGC TGTGCAGAAG 
AATAGTATTA ACCAAATGTG CCAAACATAT TCAGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_524792.1
CDS	320..1498
Translation	MDQDMGMATGYFQDADMQMDEPAAATQSIYPHSATLFAAISACVFVTIGVLGNLITLLALLKSPTIREHATTAFVISLSISDLLFCSFSLPLTAVRFFQESWTFGTTLCKIFPVIFYGNVAVSLLSMVGITLNRYILIACHSRYSQIYKPKFITLQLLFVWAVSFLLLLPPILGIWGEMGLDEATFSCTILKKEGRSIKKTLFVIGFLLPCLVIIVSYSCIYITVLHQKKKIRNHDNFQIAAAKGSSSSGGGSYMTTTCTRKAREDNRLTVMMVTIFLCFLVCFLPLMLANVVDDERNTSYPWLHIIASVMAWASSVINPIIYAASNRNYRVAYYKIFALLKFWGEPLSPMPSRNYHQSKNSKELSGVIRSTPLFHAVQKNSINQMCQTYSV

Target ORF information:

RefSeq Version	NM_080053.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster trapped in endoderm 1, transcript variant A (Tre1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_080053.4

ORF Insert Sequence:

ATGGATCAGG ACATGGGCAT GGCAACGGGC TACTTTCAGG ACGCAGACAT GCAGATGGAT 
GAACCGGCGG CCGCCACGCA ATCGATCTAC CCGCATTCGG CGACATTATT CGCGGCCATT 
AGTGCCTGTG TCTTTGTGAC GATCGGCGTT CTTGGCAACC TGATTACCCT GCTGGCGCTG 
CTCAAGAGCC CCACGATACG GGAGCATGCC ACAACCGCCT TCGTCATTTC GCTAAGCATC 
TCCGACCTGC TCTTCTGCTC CTTCAGCCTG CCACTGACCG CAGTGCGATT CTTCCAGGAG 
AGCTGGACTT TTGGTACCAC ATTGTGCAAG ATCTTTCCGG TGATCTTCTA TGGCAATGTG 
GCTGTTTCAC TCCTCAGCAT GGTGGGCATC ACCCTGAACA GATATATACT CATCGCTTGC 
CACAGCCGCT ACTCGCAGAT ATATAAGCCT AAGTTCATAA CCCTGCAGCT GTTGTTCGTT 
TGGGCCGTCT CCTTTCTGCT GTTGCTGCCG CCCATATTGG GCATCTGGGG CGAGATGGGC 
CTGGACGAGG CCACCTTCTC CTGCACAATA CTCAAGAAGG AGGGGCGATC GATCAAGAAG 
ACGCTGTTCG TGATCGGCTT CCTGCTGCCT TGCCTGGTGA TCATCGTTTC GTACTCGTGC 
ATCTACATTA CGGTGCTGCA CCAGAAAAAG AAGATTCGCA ACCACGATAA CTTCCAGATC 
GCAGCGGCCA AGGGCTCCTC GTCGTCCGGC GGCGGATCCT ACATGACCAC CACATGCACG 
CGCAAGGCGC GCGAAGACAA TCGACTGACC GTCATGATGG TCACCATCTT TCTGTGCTTC 
CTCGTCTGCT TCCTGCCGCT AATGCTGGCC AATGTGGTGG ACGACGAGCG CAATACCTCG 
TACCCCTGGC TGCACATCAT CGCCTCCGTG ATGGCCTGGG CTTCCAGTGT CATTAACCCG 
ATCATCTATG CGGCCAGCAA TCGCAACTAC AGAGTGGCCT ACTACAAGAT CTTTGCGCTG 
CTCAAGTTCT GGGGCGAACC GTTGTCGCCG ATGCCGAGTA GAAACTATCA CCAAAGCAAG 
AACTCCAAGG AGCTGTCGGG CGTCATCCGC AGCACGCCGC TGTTTCACGC TGTGCAGAAG 
AATAGTATTA ACCAAATGTG CCAAACATAT TCAGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tre1 ( NM_001258605.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001245534.1 Drosophila melanogaster trapped in endoderm 1, transcript variant B (Tre1), mRNA.

Price & Availability↑

CloneID	OFa02483
Clone ID Related Accession (Same CDS sequence)	NM_080053.4 , NM_001258605.1
Accession Version	NM_001258605.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1179bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	trapped in endoderm 1, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGATCAGG ACATGGGCAT GGCAACGGGC TACTTTCAGG ACGCAGACAT GCAGATGGAT 
GAACCGGCGG CCGCCACGCA ATCGATCTAC CCGCATTCGG CGACATTATT CGCGGCCATT 
AGTGCCTGTG TCTTTGTGAC GATCGGCGTT CTTGGCAACC TGATTACCCT GCTGGCGCTG 
CTCAAGAGCC CCACGATACG GGAGCATGCC ACAACCGCCT TCGTCATTTC GCTAAGCATC 
TCCGACCTGC TCTTCTGCTC CTTCAGCCTG CCACTGACCG CAGTGCGATT CTTCCAGGAG 
AGCTGGACTT TTGGTACCAC ATTGTGCAAG ATCTTTCCGG TGATCTTCTA TGGCAATGTG 
GCTGTTTCAC TCCTCAGCAT GGTGGGCATC ACCCTGAACA GATATATACT CATCGCTTGC 
CACAGCCGCT ACTCGCAGAT ATATAAGCCT AAGTTCATAA CCCTGCAGCT GTTGTTCGTT 
TGGGCCGTCT CCTTTCTGCT GTTGCTGCCG CCCATATTGG GCATCTGGGG CGAGATGGGC 
CTGGACGAGG CCACCTTCTC CTGCACAATA CTCAAGAAGG AGGGGCGATC GATCAAGAAG 
ACGCTGTTCG TGATCGGCTT CCTGCTGCCT TGCCTGGTGA TCATCGTTTC GTACTCGTGC 
ATCTACATTA CGGTGCTGCA CCAGAAAAAG AAGATTCGCA ACCACGATAA CTTCCAGATC 
GCAGCGGCCA AGGGCTCCTC GTCGTCCGGC GGCGGATCCT ACATGACCAC CACATGCACG 
CGCAAGGCGC GCGAAGACAA TCGACTGACC GTCATGATGG TCACCATCTT TCTGTGCTTC 
CTCGTCTGCT TCCTGCCGCT AATGCTGGCC AATGTGGTGG ACGACGAGCG CAATACCTCG 
TACCCCTGGC TGCACATCAT CGCCTCCGTG ATGGCCTGGG CTTCCAGTGT CATTAACCCG 
ATCATCTATG CGGCCAGCAA TCGCAACTAC AGAGTGGCCT ACTACAAGAT CTTTGCGCTG 
CTCAAGTTCT GGGGCGAACC GTTGTCGCCG ATGCCGAGTA GAAACTATCA CCAAAGCAAG 
AACTCCAAGG AGCTGTCGGG CGTCATCCGC AGCACGCCGC TGTTTCACGC TGTGCAGAAG 
AATAGTATTA ACCAAATGTG CCAAACATAT TCAGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001245534.1
CDS	185..1363
Translation	MDQDMGMATGYFQDADMQMDEPAAATQSIYPHSATLFAAISACVFVTIGVLGNLITLLALLKSPTIREHATTAFVISLSISDLLFCSFSLPLTAVRFFQESWTFGTTLCKIFPVIFYGNVAVSLLSMVGITLNRYILIACHSRYSQIYKPKFITLQLLFVWAVSFLLLLPPILGIWGEMGLDEATFSCTILKKEGRSIKKTLFVIGFLLPCLVIIVSYSCIYITVLHQKKKIRNHDNFQIAAAKGSSSSGGGSYMTTTCTRKAREDNRLTVMMVTIFLCFLVCFLPLMLANVVDDERNTSYPWLHIIASVMAWASSVINPIIYAASNRNYRVAYYKIFALLKFWGEPLSPMPSRNYHQSKNSKELSGVIRSTPLFHAVQKNSINQMCQTYSV

Target ORF information:

RefSeq Version	NM_001258605.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster trapped in endoderm 1, transcript variant B (Tre1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001258605.1

ORF Insert Sequence:

ATGGATCAGG ACATGGGCAT GGCAACGGGC TACTTTCAGG ACGCAGACAT GCAGATGGAT 
GAACCGGCGG CCGCCACGCA ATCGATCTAC CCGCATTCGG CGACATTATT CGCGGCCATT 
AGTGCCTGTG TCTTTGTGAC GATCGGCGTT CTTGGCAACC TGATTACCCT GCTGGCGCTG 
CTCAAGAGCC CCACGATACG GGAGCATGCC ACAACCGCCT TCGTCATTTC GCTAAGCATC 
TCCGACCTGC TCTTCTGCTC CTTCAGCCTG CCACTGACCG CAGTGCGATT CTTCCAGGAG 
AGCTGGACTT TTGGTACCAC ATTGTGCAAG ATCTTTCCGG TGATCTTCTA TGGCAATGTG 
GCTGTTTCAC TCCTCAGCAT GGTGGGCATC ACCCTGAACA GATATATACT CATCGCTTGC 
CACAGCCGCT ACTCGCAGAT ATATAAGCCT AAGTTCATAA CCCTGCAGCT GTTGTTCGTT 
TGGGCCGTCT CCTTTCTGCT GTTGCTGCCG CCCATATTGG GCATCTGGGG CGAGATGGGC 
CTGGACGAGG CCACCTTCTC CTGCACAATA CTCAAGAAGG AGGGGCGATC GATCAAGAAG 
ACGCTGTTCG TGATCGGCTT CCTGCTGCCT TGCCTGGTGA TCATCGTTTC GTACTCGTGC 
ATCTACATTA CGGTGCTGCA CCAGAAAAAG AAGATTCGCA ACCACGATAA CTTCCAGATC 
GCAGCGGCCA AGGGCTCCTC GTCGTCCGGC GGCGGATCCT ACATGACCAC CACATGCACG 
CGCAAGGCGC GCGAAGACAA TCGACTGACC GTCATGATGG TCACCATCTT TCTGTGCTTC 
CTCGTCTGCT TCCTGCCGCT AATGCTGGCC AATGTGGTGG ACGACGAGCG CAATACCTCG 
TACCCCTGGC TGCACATCAT CGCCTCCGTG ATGGCCTGGG CTTCCAGTGT CATTAACCCG 
ATCATCTATG CGGCCAGCAA TCGCAACTAC AGAGTGGCCT ACTACAAGAT CTTTGCGCTG 
CTCAAGTTCT GGGGCGAACC GTTGTCGCCG ATGCCGAGTA GAAACTATCA CCAAAGCAAG 
AACTCCAAGG AGCTGTCGGG CGTCATCCGC AGCACGCCGC TGTTTCACGC TGTGCAGAAG 
AATAGTATTA ACCAAATGTG CCAAACATAT TCAGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tre1 ( NM_001297972.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001284901.1 Drosophila melanogaster trapped in endoderm 1, transcript variant C (Tre1), mRNA.

Price & Availability↑

CloneID	OFa22292
Clone ID Related Accession (Same CDS sequence)	NM_001297972.1
Accession Version	NM_001297972.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1200bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	trapped in endoderm 1, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGATCAGG ACATGGGCAT GGCAACGGGC TACTTTCAGG ACGCAGACAT GCAGATGGAT 
GAACCGGCGG CCGCCACGCA ATCGATCTAC CCGCATTCGG CGACATTATT CGCGGCCATT 
AGTGCCTGTG TCTTTGTGAC GATCGGCGTT CTTGGCAACC TGATTACCCT GCTGGCGCTG 
CTCAAGAGCC CCACGATACG GGAGCATGCC ACAACCGCCT TCGTCATTTC GCTAAGCATC 
TCCGACCTGC TCTTCTGCTC CTTCAGCCTG CCACTGACCG CAGTGCGATT CTTCCAGGAG 
AGCTGGACTT TTGGTACCAC ATTGTGCAAG ATCTTTCCGG TGATCTTCTA TGGCAATGTG 
GCTGTTTCAC TCCTCAGCAT GGTGGGCATC ACCCTGAACA GATATATACT CATCGCTTGC 
CACAGCCGCT ACTCGCAGAT ATATAAGCCT AAGTTCATAA CCCTGCAGCT GTTGTTCGTT 
TGGGCCGTCT CCTTTCTGCT GTTGCTGCCG CCCATATTGG GCATCTGGGG CGAGATGGGC 
CTGGACGAGG CCACCTTCTC CTGCACAATA CTCAAGAAGG AGGGGCGATC GATCAAGAAG 
ACGCTGTTCG TGATCGGCTT CCTGCTGCCT TGCCTGGTGA TCATCGTTTC GTACTCGTGC 
ATCTACATTA CGGTGCTGCA CCAGAAAAAG AAGATTCGCA ACCACGATAA CTTCCAGATC 
GCAGCGGCCA AGGGCTCCTC GTCGTCCGGC GGCGGATCCT ACATGACCAC CACATGCACG 
CGCAAGGCGC GCGAAGACAA TCGACTGACC GTCATGATGG TCACCATCTT TCTGTGCTTC 
CTCGTCTGCT TCCTGCCGCT AATGCTGGCC AATGTGGTGG ACGACGAGCG CAATACCTCG 
TACCCCTGGC TGCACATCAT CGCCTCCGTG ATGGCCTGGG CTTCCAGTGT CATTAACCCG 
ATCATCTATG CGGCCAGCAA TCGCAACTAC AGCGAATCTA TCTTCTATTT CAGAGTGGCC 
TACTACAAGA TCTTTGCGCT GCTCAAGTTC TGGGGCGAAC CGTTGTCGCC GATGCCGAGT 
AGAAACTATC ACCAAAGCAA GAACTCCAAG GAGCTGTCGG GCGTCATCCG CAGCACGCCG 
CTGTTTCACG CTGTGCAGAA GAATAGTATT AACCAAATGT GCCAAACATA TTCAGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001284901.1
CDS	320..1519
Translation	MDQDMGMATGYFQDADMQMDEPAAATQSIYPHSATLFAAISACVFVTIGVLGNLITLLALLKSPTIREHATTAFVISLSISDLLFCSFSLPLTAVRFFQESWTFGTTLCKIFPVIFYGNVAVSLLSMVGITLNRYILIACHSRYSQIYKPKFITLQLLFVWAVSFLLLLPPILGIWGEMGLDEATFSCTILKKEGRSIKKTLFVIGFLLPCLVIIVSYSCIYITVLHQKKKIRNHDNFQIAAAKGSSSSGGGSYMTTTCTRKAREDNRLTVMMVTIFLCFLVCFLPLMLANVVDDERNTSYPWLHIIASVMAWASSVINPIIYAASNRNYSESIFYFRVAYYKIFALLKFWGEPLSPMPSRNYHQSKNSKELSGVIRSTPLFHAVQKNSINQMCQTYSV

Target ORF information:

RefSeq Version	NM_001297972.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster trapped in endoderm 1, transcript variant C (Tre1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001297972.1

ORF Insert Sequence:

ATGGATCAGG ACATGGGCAT GGCAACGGGC TACTTTCAGG ACGCAGACAT GCAGATGGAT 
GAACCGGCGG CCGCCACGCA ATCGATCTAC CCGCATTCGG CGACATTATT CGCGGCCATT 
AGTGCCTGTG TCTTTGTGAC GATCGGCGTT CTTGGCAACC TGATTACCCT GCTGGCGCTG 
CTCAAGAGCC CCACGATACG GGAGCATGCC ACAACCGCCT TCGTCATTTC GCTAAGCATC 
TCCGACCTGC TCTTCTGCTC CTTCAGCCTG CCACTGACCG CAGTGCGATT CTTCCAGGAG 
AGCTGGACTT TTGGTACCAC ATTGTGCAAG ATCTTTCCGG TGATCTTCTA TGGCAATGTG 
GCTGTTTCAC TCCTCAGCAT GGTGGGCATC ACCCTGAACA GATATATACT CATCGCTTGC 
CACAGCCGCT ACTCGCAGAT ATATAAGCCT AAGTTCATAA CCCTGCAGCT GTTGTTCGTT 
TGGGCCGTCT CCTTTCTGCT GTTGCTGCCG CCCATATTGG GCATCTGGGG CGAGATGGGC 
CTGGACGAGG CCACCTTCTC CTGCACAATA CTCAAGAAGG AGGGGCGATC GATCAAGAAG 
ACGCTGTTCG TGATCGGCTT CCTGCTGCCT TGCCTGGTGA TCATCGTTTC GTACTCGTGC 
ATCTACATTA CGGTGCTGCA CCAGAAAAAG AAGATTCGCA ACCACGATAA CTTCCAGATC 
GCAGCGGCCA AGGGCTCCTC GTCGTCCGGC GGCGGATCCT ACATGACCAC CACATGCACG 
CGCAAGGCGC GCGAAGACAA TCGACTGACC GTCATGATGG TCACCATCTT TCTGTGCTTC 
CTCGTCTGCT TCCTGCCGCT AATGCTGGCC AATGTGGTGG ACGACGAGCG CAATACCTCG 
TACCCCTGGC TGCACATCAT CGCCTCCGTG ATGGCCTGGG CTTCCAGTGT CATTAACCCG 
ATCATCTATG CGGCCAGCAA TCGCAACTAC AGCGAATCTA TCTTCTATTT CAGAGTGGCC 
TACTACAAGA TCTTTGCGCT GCTCAAGTTC TGGGGCGAAC CGTTGTCGCC GATGCCGAGT 
AGAAACTATC ACCAAAGCAA GAACTCCAAG GAGCTGTCGG GCGTCATCCG CAGCACGCCG 
CTGTTTCACG CTGTGCAGAA GAATAGTATT AACCAAATGT GCCAAACATA TTCAGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
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Tre1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

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ORF Insert Sequence:

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ORF Insert Sequence:

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ORF Insert Sequence:

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