HBS1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

The following HBS1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HBS1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OFa19492 NM_144472.4
Latest version!
Drosophila melanogaster HBS1, transcript variant A (HBS1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OFa19492 NM_001299977.1
Latest version!
Drosophila melanogaster HBS1, transcript variant B (HBS1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OFa19492
    Clone ID Related Accession (Same CDS sequence) NM_144472.4 , NM_001299977.1
    Accession Version NM_144472.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2013bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product HBS1, isoform A
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_144472.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGTCGCGGC ACAGGATAGT GCGCACCATG GACTACAATG ATGAGTACGA CGGCTACGAC 
    GACATCTACG GGCACTCCGT GGAGGACGAG CACTGCATCT CGCCCACGGA TGCCCAGCAA
    TGGCTCTACG ATCGGGCTCG TGGCCAGCAG AGCATCTCGG CGTTCATCAG CAAGAACAAG
    GACATCCAAG AGGAGGAGGC CGACGAGGAT GAGGACGAGG ACGCCGCCTT CGCCAAGGCT
    CGCCGCGACT CCGAGAGCTT CCAAATGCCG CAACTGGACG AGATCGAGCA GGCCAAGCTG
    AGCTCCTGCG TGGACGAGGT GCGCAGCGTG GTGGGCGATG CCGTCTCCGA GCGCCGCATC
    GTGGAGACAT CCATGAAATT CGACTATGAC ATGCAGAAGA TCCTGGACGA GATCCTCAAC
    GAAGAGACCA ACAAGTCTGC CAAGCCGGCT GTGAATAAGA TGAAAGCGCC CGCTGCTCCA
    GTGCTGCCAA AGACGGTCAG CAAAACGGTG CCTACGCCAC CACCCAAGAT CAGCCTTAAA
    GAGCCGCGAC GTGGCTTCGA AATACCCTCG CCCAAGGTGC CCTCATCGCC TGTGGTCTCG
    GGCAGAAACA CACCCGTTGA CATTAGCGCC GGCGACGACA TCTCCCGTAG CTCAGCAACC
    GTTTTCAAGG TCTCCAAGGA ACAGGCCGTG CGCAATGCCC GCCAGCTGTA CGAGAAGGAG
    CGAGCCGACC AGAAGAGCCA CATTCACATG ATTGTCATTG GTCACGTCGA CGCCGGGAAG
    AGTACGCTGA TGGGCCATCT ACTGTACGAC ACCGGCAACG TTTCGCAGCG AGTGATGCAC
    AAGCACGAGC AGGAGTCCAA GAAGCTGGGC AAGCAGAGCT TCATGTATGC CTGGGTGCTG
    GACGAGACGG GCGAGGAGCG GGCCCGCGGC ATCACCATGG ACGTGGGCCA GTCGCGTATC
    GAAACCAAGA CCAAGATCGT CACACTGCTG GATGCTCCGG GGCACAAGGA CTTCATTCCG
    AACATGATCT CCGGTGCCAC ACAGGCGGAT GTTGCCCTTC TGGTGGTGGA TGCTACAAGG
    GGAGAGTTTG AATCCGGCTT TGAGCTCGGC GGACAGACTA GAGAGCACGC CATTCTAGTG
    CGATCCCTGG GGGTTAACCA GCTAGGCGTG GTCATCAACA AGCTGGACAC CGTTGGCTGG
    TCCCAGGACC GATTCACAGA GATTGTGACC AAGCTAAAGT CCTTCCTCAA ACTGGCGGGC
    TTCAAGGATT CCGATGTGAG TTTTACGCCC TGTTCCGGAC TAACCGGCGA GAACCTCACC
    AAGAAAGCCC AGGAACCCGC CCTGACCAAC TGGTACAGTG GACGCCACCT CCTCGATGTC
    ATTGAGAACT TCAAGATCCC CGAAAGAGCC ATCGACAGAC CGCTGCGAAT GTCCGTGTCG
    GACATTTACA AGGGCACTGG CTCCGGATTC TGCATCTCGG GACGAGTGGA AACCGGAGTG
    CTGTGCCTGA ACGACAAGGT GCTGGTGGGT GCCAGCCGGG AGCAGGCCCA AGTGAAATCC
    CTTACCATGA ACGAATTCCC ACAGACTTGC GTGTTCGCCG GCGATCAGGT CTCCGTCACC
    CTGCCCGCCT TGGACATCAA CAACGTCACC GTGGGCTGCA TCATCAGCGA TCCACAAACC
    CCTATTCCGG TGACAACGCG ATTCCAGGCC CGCATAATCG TGTTCAACGT CAAGGTGCCC
    ATTACCATGG GCTTCCCCGT GCTGCTGCAC CACCAATCCC TAATAGAGCC CGCCGTGGTC
    TGCAAGCTGA CCGCATCGAT TCACAAGAGC ACCGGCGAGG TGGTCAAGAA GAAGCCGCGT
    TGCCTGGGCA ACAACTCGTG TGCCCTAGTG GAACTGGAAA CCAGCAGACC CATCTGCATC
    GAGCGGTATG CGGACTTCAA GGAGCTGGGA CGCGTGATGC TTCGCGTGGC GGGAGTCACC
    ATTGCCGCCG GAATGGTCAC CAAGATCCGC TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_652729.2
    CDS95..2107
    Translation

    Target ORF information:

    RefSeq Version NM_144472.4
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster HBS1, transcript variant A (HBS1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_144472.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGTCGCGGC ACAGGATAGT GCGCACCATG GACTACAATG ATGAGTACGA CGGCTACGAC 
    GACATCTACG GGCACTCCGT GGAGGACGAG CACTGCATCT CGCCCACGGA TGCCCAGCAA
    TGGCTCTACG ATCGGGCTCG TGGCCAGCAG AGCATCTCGG CGTTCATCAG CAAGAACAAG
    GACATCCAAG AGGAGGAGGC CGACGAGGAT GAGGACGAGG ACGCCGCCTT CGCCAAGGCT
    CGCCGCGACT CCGAGAGCTT CCAAATGCCG CAACTGGACG AGATCGAGCA GGCCAAGCTG
    AGCTCCTGCG TGGACGAGGT GCGCAGCGTG GTGGGCGATG CCGTCTCCGA GCGCCGCATC
    GTGGAGACAT CCATGAAATT CGACTATGAC ATGCAGAAGA TCCTGGACGA GATCCTCAAC
    GAAGAGACCA ACAAGTCTGC CAAGCCGGCT GTGAATAAGA TGAAAGCGCC CGCTGCTCCA
    GTGCTGCCAA AGACGGTCAG CAAAACGGTG CCTACGCCAC CACCCAAGAT CAGCCTTAAA
    GAGCCGCGAC GTGGCTTCGA AATACCCTCG CCCAAGGTGC CCTCATCGCC TGTGGTCTCG
    GGCAGAAACA CACCCGTTGA CATTAGCGCC GGCGACGACA TCTCCCGTAG CTCAGCAACC
    GTTTTCAAGG TCTCCAAGGA ACAGGCCGTG CGCAATGCCC GCCAGCTGTA CGAGAAGGAG
    CGAGCCGACC AGAAGAGCCA CATTCACATG ATTGTCATTG GTCACGTCGA CGCCGGGAAG
    AGTACGCTGA TGGGCCATCT ACTGTACGAC ACCGGCAACG TTTCGCAGCG AGTGATGCAC
    AAGCACGAGC AGGAGTCCAA GAAGCTGGGC AAGCAGAGCT TCATGTATGC CTGGGTGCTG
    GACGAGACGG GCGAGGAGCG GGCCCGCGGC ATCACCATGG ACGTGGGCCA GTCGCGTATC
    GAAACCAAGA CCAAGATCGT CACACTGCTG GATGCTCCGG GGCACAAGGA CTTCATTCCG
    AACATGATCT CCGGTGCCAC ACAGGCGGAT GTTGCCCTTC TGGTGGTGGA TGCTACAAGG
    GGAGAGTTTG AATCCGGCTT TGAGCTCGGC GGACAGACTA GAGAGCACGC CATTCTAGTG
    CGATCCCTGG GGGTTAACCA GCTAGGCGTG GTCATCAACA AGCTGGACAC CGTTGGCTGG
    TCCCAGGACC GATTCACAGA GATTGTGACC AAGCTAAAGT CCTTCCTCAA ACTGGCGGGC
    TTCAAGGATT CCGATGTGAG TTTTACGCCC TGTTCCGGAC TAACCGGCGA GAACCTCACC
    AAGAAAGCCC AGGAACCCGC CCTGACCAAC TGGTACAGTG GACGCCACCT CCTCGATGTC
    ATTGAGAACT TCAAGATCCC CGAAAGAGCC ATCGACAGAC CGCTGCGAAT GTCCGTGTCG
    GACATTTACA AGGGCACTGG CTCCGGATTC TGCATCTCGG GACGAGTGGA AACCGGAGTG
    CTGTGCCTGA ACGACAAGGT GCTGGTGGGT GCCAGCCGGG AGCAGGCCCA AGTGAAATCC
    CTTACCATGA ACGAATTCCC ACAGACTTGC GTGTTCGCCG GCGATCAGGT CTCCGTCACC
    CTGCCCGCCT TGGACATCAA CAACGTCACC GTGGGCTGCA TCATCAGCGA TCCACAAACC
    CCTATTCCGG TGACAACGCG ATTCCAGGCC CGCATAATCG TGTTCAACGT CAAGGTGCCC
    ATTACCATGG GCTTCCCCGT GCTGCTGCAC CACCAATCCC TAATAGAGCC CGCCGTGGTC
    TGCAAGCTGA CCGCATCGAT TCACAAGAGC ACCGGCGAGG TGGTCAAGAA GAAGCCGCGT
    TGCCTGGGCA ACAACTCGTG TGCCCTAGTG GAACTGGAAA CCAGCAGACC CATCTGCATC
    GAGCGGTATG CGGACTTCAA GGAGCTGGGA CGCGTGATGC TTCGCGTGGC GGGAGTCACC
    ATTGCCGCCG GAATGGTCAC CAAGATCCGC TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa19492
    Clone ID Related Accession (Same CDS sequence) NM_144472.4 , NM_001299977.1
    Accession Version NM_001299977.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2013bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product HBS1, isoform B
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGTCGCGGC ACAGGATAGT GCGCACCATG GACTACAATG ATGAGTACGA CGGCTACGAC 
    GACATCTACG GGCACTCCGT GGAGGACGAG CACTGCATCT CGCCCACGGA TGCCCAGCAA
    TGGCTCTACG ATCGGGCTCG TGGCCAGCAG AGCATCTCGG CGTTCATCAG CAAGAACAAG
    GACATCCAAG AGGAGGAGGC CGACGAGGAT GAGGACGAGG ACGCCGCCTT CGCCAAGGCT
    CGCCGCGACT CCGAGAGCTT CCAAATGCCG CAACTGGACG AGATCGAGCA GGCCAAGCTG
    AGCTCCTGCG TGGACGAGGT GCGCAGCGTG GTGGGCGATG CCGTCTCCGA GCGCCGCATC
    GTGGAGACAT CCATGAAATT CGACTATGAC ATGCAGAAGA TCCTGGACGA GATCCTCAAC
    GAAGAGACCA ACAAGTCTGC CAAGCCGGCT GTGAATAAGA TGAAAGCGCC CGCTGCTCCA
    GTGCTGCCAA AGACGGTCAG CAAAACGGTG CCTACGCCAC CACCCAAGAT CAGCCTTAAA
    GAGCCGCGAC GTGGCTTCGA AATACCCTCG CCCAAGGTGC CCTCATCGCC TGTGGTCTCG
    GGCAGAAACA CACCCGTTGA CATTAGCGCC GGCGACGACA TCTCCCGTAG CTCAGCAACC
    GTTTTCAAGG TCTCCAAGGA ACAGGCCGTG CGCAATGCCC GCCAGCTGTA CGAGAAGGAG
    CGAGCCGACC AGAAGAGCCA CATTCACATG ATTGTCATTG GTCACGTCGA CGCCGGGAAG
    AGTACGCTGA TGGGCCATCT ACTGTACGAC ACCGGCAACG TTTCGCAGCG AGTGATGCAC
    AAGCACGAGC AGGAGTCCAA GAAGCTGGGC AAGCAGAGCT TCATGTATGC CTGGGTGCTG
    GACGAGACGG GCGAGGAGCG GGCCCGCGGC ATCACCATGG ACGTGGGCCA GTCGCGTATC
    GAAACCAAGA CCAAGATCGT CACACTGCTG GATGCTCCGG GGCACAAGGA CTTCATTCCG
    AACATGATCT CCGGTGCCAC ACAGGCGGAT GTTGCCCTTC TGGTGGTGGA TGCTACAAGG
    GGAGAGTTTG AATCCGGCTT TGAGCTCGGC GGACAGACTA GAGAGCACGC CATTCTAGTG
    CGATCCCTGG GGGTTAACCA GCTAGGCGTG GTCATCAACA AGCTGGACAC CGTTGGCTGG
    TCCCAGGACC GATTCACAGA GATTGTGACC AAGCTAAAGT CCTTCCTCAA ACTGGCGGGC
    TTCAAGGATT CCGATGTGAG TTTTACGCCC TGTTCCGGAC TAACCGGCGA GAACCTCACC
    AAGAAAGCCC AGGAACCCGC CCTGACCAAC TGGTACAGTG GACGCCACCT CCTCGATGTC
    ATTGAGAACT TCAAGATCCC CGAAAGAGCC ATCGACAGAC CGCTGCGAAT GTCCGTGTCG
    GACATTTACA AGGGCACTGG CTCCGGATTC TGCATCTCGG GACGAGTGGA AACCGGAGTG
    CTGTGCCTGA ACGACAAGGT GCTGGTGGGT GCCAGCCGGG AGCAGGCCCA AGTGAAATCC
    CTTACCATGA ACGAATTCCC ACAGACTTGC GTGTTCGCCG GCGATCAGGT CTCCGTCACC
    CTGCCCGCCT TGGACATCAA CAACGTCACC GTGGGCTGCA TCATCAGCGA TCCACAAACC
    CCTATTCCGG TGACAACGCG ATTCCAGGCC CGCATAATCG TGTTCAACGT CAAGGTGCCC
    ATTACCATGG GCTTCCCCGT GCTGCTGCAC CACCAATCCC TAATAGAGCC CGCCGTGGTC
    TGCAAGCTGA CCGCATCGAT TCACAAGAGC ACCGGCGAGG TGGTCAAGAA GAAGCCGCGT
    TGCCTGGGCA ACAACTCGTG TGCCCTAGTG GAACTGGAAA CCAGCAGACC CATCTGCATC
    GAGCGGTATG CGGACTTCAA GGAGCTGGGA CGCGTGATGC TTCGCGTGGC GGGAGTCACC
    ATTGCCGCCG GAATGGTCAC CAAGATCCGC TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001286906.1
    CDS196..2208
    Translation

    Target ORF information:

    RefSeq Version NM_001299977.1
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster HBS1, transcript variant B (HBS1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001299977.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGTCGCGGC ACAGGATAGT GCGCACCATG GACTACAATG ATGAGTACGA CGGCTACGAC 
    GACATCTACG GGCACTCCGT GGAGGACGAG CACTGCATCT CGCCCACGGA TGCCCAGCAA
    TGGCTCTACG ATCGGGCTCG TGGCCAGCAG AGCATCTCGG CGTTCATCAG CAAGAACAAG
    GACATCCAAG AGGAGGAGGC CGACGAGGAT GAGGACGAGG ACGCCGCCTT CGCCAAGGCT
    CGCCGCGACT CCGAGAGCTT CCAAATGCCG CAACTGGACG AGATCGAGCA GGCCAAGCTG
    AGCTCCTGCG TGGACGAGGT GCGCAGCGTG GTGGGCGATG CCGTCTCCGA GCGCCGCATC
    GTGGAGACAT CCATGAAATT CGACTATGAC ATGCAGAAGA TCCTGGACGA GATCCTCAAC
    GAAGAGACCA ACAAGTCTGC CAAGCCGGCT GTGAATAAGA TGAAAGCGCC CGCTGCTCCA
    GTGCTGCCAA AGACGGTCAG CAAAACGGTG CCTACGCCAC CACCCAAGAT CAGCCTTAAA
    GAGCCGCGAC GTGGCTTCGA AATACCCTCG CCCAAGGTGC CCTCATCGCC TGTGGTCTCG
    GGCAGAAACA CACCCGTTGA CATTAGCGCC GGCGACGACA TCTCCCGTAG CTCAGCAACC
    GTTTTCAAGG TCTCCAAGGA ACAGGCCGTG CGCAATGCCC GCCAGCTGTA CGAGAAGGAG
    CGAGCCGACC AGAAGAGCCA CATTCACATG ATTGTCATTG GTCACGTCGA CGCCGGGAAG
    AGTACGCTGA TGGGCCATCT ACTGTACGAC ACCGGCAACG TTTCGCAGCG AGTGATGCAC
    AAGCACGAGC AGGAGTCCAA GAAGCTGGGC AAGCAGAGCT TCATGTATGC CTGGGTGCTG
    GACGAGACGG GCGAGGAGCG GGCCCGCGGC ATCACCATGG ACGTGGGCCA GTCGCGTATC
    GAAACCAAGA CCAAGATCGT CACACTGCTG GATGCTCCGG GGCACAAGGA CTTCATTCCG
    AACATGATCT CCGGTGCCAC ACAGGCGGAT GTTGCCCTTC TGGTGGTGGA TGCTACAAGG
    GGAGAGTTTG AATCCGGCTT TGAGCTCGGC GGACAGACTA GAGAGCACGC CATTCTAGTG
    CGATCCCTGG GGGTTAACCA GCTAGGCGTG GTCATCAACA AGCTGGACAC CGTTGGCTGG
    TCCCAGGACC GATTCACAGA GATTGTGACC AAGCTAAAGT CCTTCCTCAA ACTGGCGGGC
    TTCAAGGATT CCGATGTGAG TTTTACGCCC TGTTCCGGAC TAACCGGCGA GAACCTCACC
    AAGAAAGCCC AGGAACCCGC CCTGACCAAC TGGTACAGTG GACGCCACCT CCTCGATGTC
    ATTGAGAACT TCAAGATCCC CGAAAGAGCC ATCGACAGAC CGCTGCGAAT GTCCGTGTCG
    GACATTTACA AGGGCACTGG CTCCGGATTC TGCATCTCGG GACGAGTGGA AACCGGAGTG
    CTGTGCCTGA ACGACAAGGT GCTGGTGGGT GCCAGCCGGG AGCAGGCCCA AGTGAAATCC
    CTTACCATGA ACGAATTCCC ACAGACTTGC GTGTTCGCCG GCGATCAGGT CTCCGTCACC
    CTGCCCGCCT TGGACATCAA CAACGTCACC GTGGGCTGCA TCATCAGCGA TCCACAAACC
    CCTATTCCGG TGACAACGCG ATTCCAGGCC CGCATAATCG TGTTCAACGT CAAGGTGCCC
    ATTACCATGG GCTTCCCCGT GCTGCTGCAC CACCAATCCC TAATAGAGCC CGCCGTGGTC
    TGCAAGCTGA CCGCATCGAT TCACAAGAGC ACCGGCGAGG TGGTCAAGAA GAAGCCGCGT
    TGCCTGGGCA ACAACTCGTG TGCCCTAGTG GAACTGGAAA CCAGCAGACC CATCTGCATC
    GAGCGGTATG CGGACTTCAA GGAGCTGGGA CGCGTGATGC TTCGCGTGGC GGGAGTCACC
    ATTGCCGCCG GAATGGTCAC CAAGATCCGC TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.