Rheb cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Rheb
Entrez Gene ID	117332
Full Name	Ras homolog enriched in brain
Synonyms	CG1081,DReb,Dm Rheb,DmRheb,Dmel\CG1081,Q9VND8,RHEB,RheB,dRheb,rheb
General protein information	Preferred Names Ras homolog enriched in brain Names CG1081 gene product from transcript CG1081-RA CG1081-PA CG1081-PB RAS homolog enriched in brain Ras homolog enriched in brain ortholog Ras homologue enriched in brain Ras homology enriched in brain Rheb-PA Rheb-PB
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 83B2-83B2

mRNA and Protein(s)

mRNA	Protein	Name
NM_169068.3	NP_730950.2	Ras homolog enriched in brain, isoform A
NM_169069.3	NP_730951.2	Ras homolog enriched in brain, isoform B

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	rheb	NP_001070216.1
Drosophila melanogaster (fruit fly)	Rheb	NP_730951.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001902	XP_321164.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Rheb overexpression in the central brain neurons of flies causes not only morphological phenotypes, but behavioral and aging phenotypes that may mirror symptoms of tuberous sclerosis complex
Title: The small GTPase Rheb affects central brain neuronal morphology and memory formation in Drosophila.

Drosophila TCTP (dTCTP) displays GEF activity to Rheb and is essential for Rheb activation in organ growth
Title: To cease or to proliferate: new insights into TCTP function from a Drosophila study.

Rheb-TOR signaling controls S2 cell growth by promoting ribosome production and protein synthesis, not by direct effects on the import of amino acids or glucose.
Title: Rheb-TOR signaling promotes protein synthesis, but not glucose or amino acid import, in Drosophila.

Our studies identify TCTP as a direct regulator of Rheb and a potential therapeutic target for tuberous sclerosis disease.
Title: Drosophila TCTP is essential for growth and proliferation through regulation of dRheb GTPase.

Results show that mildly increasing systemic Rheb-TOR-S6K signaling sensitizes the whole organism to oxidative stress and promotes senescence of locomotor activity with age.
Title: Increased Rheb-TOR signaling enhances sensitivity of the whole organism to oxidative stress.

The following Rheb gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Rheb cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa07041	NM_169068.3 Latest version!	Drosophila melanogaster Ras homolog enriched in brain (Rheb), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OFa07041	NM_169069.3 Latest version!	Drosophila melanogaster Ras homolog enriched in brain (Rheb), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
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5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Rheb ( NM_169068.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_730950.2 Drosophila melanogaster Ras homolog enriched in brain (Rheb), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa07041
Clone ID Related Accession (Same CDS sequence)	NM_169068.3 , NM_169069.3
Accession Version	NM_169068.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 549bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Ras homolog enriched in brain, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_169068.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCAACCA AGGAGCGCCA CATAGCCATG ATGGGCTACC GATCAGTGGG CAAATCGTCG 
CTATGCATAC AGTTCGTGGA GGGCCAGTTC GTGGACTCCT ATGACCCCAC CATTGAGAAC 
ACCTTCACCA AGATCGAGCG TGTAAAGTCG CAAGACTACA TCGTAAAGCT CATCGACACG 
GCGGGCCAGG ACGAGTACAG CATATTCCCG GTGCAGTACA GCATGGATTA CCACGGCTAC 
GTCCTGGTGT ACTCGATAAC CAGCCAAAAG TCTTTTGAGG TGGTGAAGAT CATATACGAA 
AAACTGCTCG ACGTAATGGG CAAGAAATAT GTACCTGTGG TGTTGGTCGG CAACAAGATC 
GATCTCCACC AGGAACGAAC CGTTTCCACG GAGGAAGGCA AGAAGCTGGC CGAGTCCTGG 
CGAGCTGCAT TTCTCGAAAC GTCCGCCAAA CAGAACGAGT CCGTGGGAGA TATATTCCAT 
CAGCTACTGA TCCTCATCGA AAACGAGAAC GGCAATCCCC AGGAGAAGAG CGGTTGTCTT 
GTATCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_730950.2
CDS	303..851
Translation	MPTKERHIAMMGYRSVGKSSLCIQFVEGQFVDSYDPTIENTFTKIERVKSQDYIVKLIDTAGQDEYSIFPVQYSMDYHGYVLVYSITSQKSFEVVKIIYEKLLDVMGKKYVPVVLVGNKIDLHQERTVSTEEGKKLAESWRAAFLETSAKQNESVGDIFHQLLILIENENGNPQEKSGCLVS

Target ORF information:

RefSeq Version	NM_169068.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Ras homolog enriched in brain (Rheb), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_169068.3

ORF Insert Sequence:

ATGCCAACCA AGGAGCGCCA CATAGCCATG ATGGGCTACC GATCAGTGGG CAAATCGTCG 
CTATGCATAC AGTTCGTGGA GGGCCAGTTC GTGGACTCCT ATGACCCCAC CATTGAGAAC 
ACCTTCACCA AGATCGAGCG TGTAAAGTCG CAAGACTACA TCGTAAAGCT CATCGACACG 
GCGGGCCAGG ACGAGTACAG CATATTCCCG GTGCAGTACA GCATGGATTA CCACGGCTAC 
GTCCTGGTGT ACTCGATAAC CAGCCAAAAG TCTTTTGAGG TGGTGAAGAT CATATACGAA 
AAACTGCTCG ACGTAATGGG CAAGAAATAT GTACCTGTGG TGTTGGTCGG CAACAAGATC 
GATCTCCACC AGGAACGAAC CGTTTCCACG GAGGAAGGCA AGAAGCTGGC CGAGTCCTGG 
CGAGCTGCAT TTCTCGAAAC GTCCGCCAAA CAGAACGAGT CCGTGGGAGA TATATTCCAT 
CAGCTACTGA TCCTCATCGA AAACGAGAAC GGCAATCCCC AGGAGAAGAG CGGTTGTCTT 
GTATCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rheb ( NM_169069.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_730951.2 Drosophila melanogaster Ras homolog enriched in brain (Rheb), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa07041
Clone ID Related Accession (Same CDS sequence)	NM_169068.3 , NM_169069.3
Accession Version	NM_169069.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 549bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Ras homolog enriched in brain, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_169069.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCAACCA AGGAGCGCCA CATAGCCATG ATGGGCTACC GATCAGTGGG CAAATCGTCG 
CTATGCATAC AGTTCGTGGA GGGCCAGTTC GTGGACTCCT ATGACCCCAC CATTGAGAAC 
ACCTTCACCA AGATCGAGCG TGTAAAGTCG CAAGACTACA TCGTAAAGCT CATCGACACG 
GCGGGCCAGG ACGAGTACAG CATATTCCCG GTGCAGTACA GCATGGATTA CCACGGCTAC 
GTCCTGGTGT ACTCGATAAC CAGCCAAAAG TCTTTTGAGG TGGTGAAGAT CATATACGAA 
AAACTGCTCG ACGTAATGGG CAAGAAATAT GTACCTGTGG TGTTGGTCGG CAACAAGATC 
GATCTCCACC AGGAACGAAC CGTTTCCACG GAGGAAGGCA AGAAGCTGGC CGAGTCCTGG 
CGAGCTGCAT TTCTCGAAAC GTCCGCCAAA CAGAACGAGT CCGTGGGAGA TATATTCCAT 
CAGCTACTGA TCCTCATCGA AAACGAGAAC GGCAATCCCC AGGAGAAGAG CGGTTGTCTT 
GTATCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_730951.2
CDS	137..685
Translation	MPTKERHIAMMGYRSVGKSSLCIQFVEGQFVDSYDPTIENTFTKIERVKSQDYIVKLIDTAGQDEYSIFPVQYSMDYHGYVLVYSITSQKSFEVVKIIYEKLLDVMGKKYVPVVLVGNKIDLHQERTVSTEEGKKLAESWRAAFLETSAKQNESVGDIFHQLLILIENENGNPQEKSGCLVS

Target ORF information:

RefSeq Version	NM_169069.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Ras homolog enriched in brain (Rheb), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_169069.3

ORF Insert Sequence:

ATGCCAACCA AGGAGCGCCA CATAGCCATG ATGGGCTACC GATCAGTGGG CAAATCGTCG 
CTATGCATAC AGTTCGTGGA GGGCCAGTTC GTGGACTCCT ATGACCCCAC CATTGAGAAC 
ACCTTCACCA AGATCGAGCG TGTAAAGTCG CAAGACTACA TCGTAAAGCT CATCGACACG 
GCGGGCCAGG ACGAGTACAG CATATTCCCG GTGCAGTACA GCATGGATTA CCACGGCTAC 
GTCCTGGTGT ACTCGATAAC CAGCCAAAAG TCTTTTGAGG TGGTGAAGAT CATATACGAA 
AAACTGCTCG ACGTAATGGG CAAGAAATAT GTACCTGTGG TGTTGGTCGG CAACAAGATC 
GATCTCCACC AGGAACGAAC CGTTTCCACG GAGGAAGGCA AGAAGCTGGC CGAGTCCTGG 
CGAGCTGCAT TTCTCGAAAC GTCCGCCAAA CAGAACGAGT CCGTGGGAGA TATATTCCAT 
CAGCTACTGA TCCTCATCGA AAACGAGAAC GGCAATCCCC AGGAGAAGAG CGGTTGTCTT 
GTATCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Rheb cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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