ncoa2 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	ncoa2
Entrez Gene ID	81884
Full Name	nuclear receptor coactivator 2
Synonyms	tif2,wu:fc41a10,wu:fe26a11
General protein information	Preferred Names nuclear receptor coactivator 2 Names nuclear receptor coactivator 2 NCoA2 grip1 src2
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009297338.2	XP_009295613.1	nuclear receptor coactivator 2 isoform X3
XM_009297338.3	XP_009295613.1	nuclear receptor coactivator 2 isoform X3
NM_131777.1	NP_571852.1	nuclear receptor coactivator 2
XM_009297337.2	XP_009295612.1	nuclear receptor coactivator 2 isoform X2
XM_009297336.2	XP_009295611.1	nuclear receptor coactivator 2 isoform X1
XM_009297336.3	XP_009295611.1	nuclear receptor coactivator 2 isoform X1
XM_021470228.1	XP_021325903.1	nuclear receptor coactivator 2 isoform X2

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	NCOA2	XP_001163706.1
Rattus norvegicus (Norway rat)	Ncoa2	NP_114010.1
Gallus gallus (chicken)	NCOA2	XP_001231578.2
Danio rerio (zebrafish)	ncoa2	NP_571852.1
	NCOA2	XP_001082161.1
Mus musculus (house mouse)	Ncoa2	NP_032704.2
Canis lupus familiaris (dog)	NCOA2	XP_544118.2
Bos taurus (cattle)	NCOA2	XP_005215601.1
Homo sapiens (human)	NCOA2	NP_006531.1
Xenopus tropicalis (tropical clawed frog)	ncoa2	NP_001135631.1

Gene Ontology
Bibliography

Related articles in PubMed

Pharmacokinetics and effects of tetrabromobisphenol a (TBBPA) to early life stages of zebrafish (Danio rerio).
Liu H, Ma Z, Zhang T, Yu N, Su G, Giesy JP, Yu H
Chemosphere190243-252(2018 Jan)

Integrated in silico and in vivo approaches to investigate effects of BDE-99 mediated by the nuclear receptors on developing zebrafish.
Zhang L, Jin Y, Han Z, Liu H, Shi L, Hua X, Doering JA, Tang S, Giesy JP, Yu H
Environmental toxicology and chemistry(2017 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The tif2 is involved in embryogenesis and in primitive hematopoiesis. tif2-knockdown zebrafish embryos are smaller than controls.
Title: A role for the transcription intermediary factor 2 in zebrafish myelopoiesis.

The following ncoa2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ncoa2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa37427	NM_131777.1 Latest version!	Danio rerio nuclear receptor coactivator 2 (ncoa2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
ODa39440	XM_009297336.3 Latest version!	Danio rerio nuclear receptor coactivator 2 (ncoa2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
ODa39441	XM_021470228.1 Latest version!	Danio rerio nuclear receptor coactivator 2 (ncoa2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
ODa39441	XM_009297337.2 Latest version!	Danio rerio nuclear receptor coactivator 2 (ncoa2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
ODa39442	XM_009297338.3 Latest version!	Danio rerio nuclear receptor coactivator 2 (ncoa2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
View Old Accession Versions >>
ODa39442	XM_009297338.2	Danio rerio nuclear receptor coactivator 2 (ncoa2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
ODa39440	XM_009297336.2	Danio rerio nuclear receptor coactivator 2 (ncoa2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ncoa2 ( NM_131777.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_571852.1 Danio rerio nuclear receptor coactivator 2 (ncoa2), mRNA.

Price & Availability↑

CloneID	ODa37427
Clone ID Related Accession (Same CDS sequence)	NM_131777.1
Accession Version	NM_131777.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4518bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Danio rerio(zebrafish)
Product	nuclear receptor coactivator 2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF323989.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF323989.1, BC163724.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGAGTGCTG TCGGGGAAAA CTCGTCTGAC CCTGCCAGGT CAGAGGCTCA GAAGCGCAAG 
GAAGGCCCAT CAGACCTCCT GGGTCCAAGT CCCAAACGAA GTACAGAAAA ACGTAATCGG 
GAACATGAAA GTAAGTACAT TGAGGAGCTC GCCGAGCTGA TTTTCGCAAA CTTCAATGAC 
ATTGACAACT TCAACGTCAA GCCTGACAAA TGTGCAATCC TAAAGGAAAC CGTGAAACAG 
ATTCGGCAAA TTAAAGAGCA AGAGAAAGCT GCTGCAGCCA ATGAGGATGA AGTGCAGAAG 
GCTGATGTGT CGTCGACAGG CCAAAGTGTT ATTGACAAAG ATGCCCTTGG ACCAATGATG 
CTGGAGGCCT TGGATGGGTT CTTCTTTGTG GTGAACATGG AGGGCAACAT TGTGTTTGTA 
TCGGAGAATG TGACGCAGTA CCTGCGCTAT AATCAGGAAG AGCTGATGAA CACCAGTGTG 
TACAGCATAC TGCATGTAGG AGACCATGCC GAATTCATCA AAAACCTCCT GCCTAAGTCA 
CACGTGAATG GAGTGCCGTG GTCCAGCGAA AACCCTCGGC GGAACAGCCA CACCTTCAAC 
TGCCGCATGC TTGTGAACCC TCACAGCGAG GCCGAAGAGA CGCAGGATCA CGAGGCACAG 
CAGAAATATG AGACTATGCA GTGTTTTGCT GTGTCTGAAC CAAAGTCCAT CAAGGAGGAG 
GGAGAGGATT TCCAGTCGTG TCTGATCTGC GTGGCGCGGC GAGTGCCCAT GAAAGAGAGA 
CCGATGCTCC CCACGCAGGA GAGCTTCACC ACCCGCCAGG ACCTCCAAGG TAAAATCACG 
TCTCTGGACA CAAGTCTGTT ACGAGCGTCT ATGAAGCCAG GCTGGGAAGA CTTGGTGCGG 
AGATGCATTC AGCGTTTTCA TCTTCAGAAC GATGGAGACA TTTCCTTCGC CAAACGACAT 
CAGCAAGAAG TGATAAGGCA CGGGCAGGCG TTCAGTCCCA TCTACCGGTT CTCCCTGTCA 
GACGGGACCA TCGTCTCTGC TCACACCAAG AGCAAACTGG TTCGCTCATC CTCTACCAAC 
GAGCCACAGC TCTACATGTC CCTGCACATC TTACAGAGGG AGCAGCCCGT CTGTGGCATG 
GCCTCTGATC TTGGAAATGC TCAGACCATG GGAAAACCCA TGAACCCCAT GAGCTCTCCA 
AACACAGCGG GTTCCTCGTG CACTCCCCAA GGGCAGGACG CCACCATCAG CAGCAACACA 
TCCACTTTCC CCTCCCCTGG TGCCCAGAAG GAGCCTGGTG CCATGCACCG TTTCGGCTGC 
CCGGGAACCA TGAGCCATTC GGCAACCATG CAAGCGGCCA CCCCTCAGGG CAGCGGGTAT 
CCACTGAAGC TGAGCAGCCC GTCTCAGGGT AGTCCGGGCA TGCTTTCTCC ACGTCACCGA 
GCCAGTCCTG GAGTAGCAGG ATCTCCACGC TTACCCCCGC CACAGTTCTC TCCAGCGGGA 
AGCCTCCATT CACCTGCCAG CATGTGCACT AGCAGTACTG GTGGCAATGG GGGAGCAGGG 
GCCAACCATG GTTACACAAG CAGTTCACTT AATGCTCTTC AGGCGCTTAG CGAATGCCAT 
GGCGTAAGCC ACGGACAATC CCTGGGATCG CCTGATAGAA AGTTAGGGTC CCCAGCTGCA 
CATTCGGTAG CAGTAAGTCA TCACCTAATT AACAAAATGA GCGTACCTGA GTCATTCGGA 
GAGCAGAACC AATCAGAGCA GGGCACCCCT GGACAGGATG AAGGCATTGA TCTGCCAAGA 
GATGAAAAGG GCAACCTCGG GCAGTTCGGC AACCTGGACG GCAGTGATCC TCAAAGCCGA 
CTGCGCGACA ACAAAAGTCA CACTAAACTC CTGCAGCTCC TCACCACTAA AACTGAGCCT 
ATCGAGTCCA CTTCACCCCC TCCAATGGCC GGTGGAGAAC CTGGGTGTAA AGACGGCGGT 
GCAGGGAATG GTGGCATGGG TTCTCAAGGC GGAAACGGGT CACATGCTAC CTCCCTGAAG 
GAGAAACACA AAATCCTTCA CAGACTCCTC CAGAACAGCT CGTCGCCTGT TGATTTGGCC 
AAACTTACAG CTGAGGCCAC AGGGAAAGAG CTGTGTCAGG ATTCTGCTGG CGGGACGGCC 
GGTGTGCCCG AGTTGGCCAT TAAACAAGAG CCTGTCAGTC CCAAGAAAAA GGACAATGCC 
TTGCTGCGCT ACCTACTGGA TAAAGATGAC AATGTACTCA AGGGTAAAGG TATTAAAATG 
GAGCCTGGAG AGATCAAGGA GGAGGGGGGC AAGATGACTG GGATTAAGAC CGAGAAAACT 
GATGGTGGCT ACGACCGTGT GGAGCCGTCA AGTGAGCTGG ATGATATCCT GGATGATCTT 
CAGAACAGTC AGCCGGGCCT GTTCACTGAC TCCAGGCCTG TGTCTCTGCC TTCTGCTGTG 
GACAAGCAGT CAATCATCAA TGACATCCTG CAGATGACAG GAGAGAGCGG AGCTAACATG 
AGTCCTCAAC AGCAGAGAGC ACTGCAGACT GCAGTCAGCC AACAGAATTT CCCTGCTCCA 
CGGCCCGGTC AGCCTGGCAG AGCTCCTCCG GTCAGAAGCG TGTCTCTGGA CATGAACATG 
CCTCCTAAAG GCGCTCAGGG TCAGTATCCT ATGATGAGGA ACAACAACCC CTACTCAATC 
ATGCAGCAGC AAGGCATGAT GGGAAACCAC GCCGTCATGC CCAATCAGCC CAACATGGTG 
AACGCAGGAG TCATGGGTAA TAATGGTCCC CGTGTAGGCA TGCAGCAGGA CGGTTGGGGT 
GCTCAGGGTA CCGTCGGCAG CGCAGCTTCA CCTGCCACAG CTGCTCCCGC TGGCCAGCAC 
TCTCTCCCGC AGGGGGCACT GCACAGCCGC ATGGTGCCCA ACCCTGCCAC AGGAATGCCC 
ATGAGACCCA ACAGCCAGCC TGGACCCAGA CCAATGCTGC AACCGCAGAT GATGGCCAAT 
GCTCAGTCAA ACATGGACAT GGGCATGGCT GGCCACCAGT TTCCCCAGCA ACAGGCACCG 
CCCAATCAGA CGGCTCCATG GCCAGACAGT GTGATGCCTA TAGAGCCGGT GCCGTTTGGG 
AACCAGAGCA GTTCCATGTA CGGCGGGTCT CAGGAGGATG TGTTGTGCCC TCCTGCCTCT 
GAAGGTCCGG CTGATGAGGG GGCTTTACTC AGCCAGCTCT ACTCGGCCCT CAAAGATTTT 
GATGGTCTGG AGGAGATTGA CCGTGCGCTT GGCATTCCAG CGCTAGTGGG ACAGGCTCAG 
CCTGTGGAGC AGGATCAGTT CCCTGGTCAG GACCCTAGTA TGATGATGGA TCAGAAGCCA 
CCGATGTACG GGCAGCAACA GTATGCCTCG TCCCCGGCAA ACATGCAGCA GGCTGGATAC 
ACGCCAATGC AAGATGCCAC TTTCCACGGC TTGCAAGGAC AGATGGGACA GAGACCCGGA 
TACCCAATGC TCCGAATGCA AGCGAGACCG GGACTCAGAC CCGGTGGAGT CGTCCCAAAT 
CAACCCAATA CACTCAGACT GCAGCTTCAG CACAGGCTAC AAGCACAGCA GAATCGTCAG 
CCTATGATGA ATCAGATGGG TGGAGTGTCA AATATGAACC TACCTCTCAG ACCCAACGTT 
CCAAATCAGG GGACAATCAA CGCTCAGATG CTGGCCCAGC GGCAGAGAGA GTTGCTGAGT 
AATCATTTAC GTCAGCGGCA GCTCGACCAG CAGCGGCAGC AACAGCAACA GCAGCAGCAG 
CAGCGCAGTC TGGCCATGCG GGCGCAGGGC CTCACCATGC CACCCAACAT GGCGGCAGCC 
GCAGCGGGCA TGCCAGGAGC CATGAGCAAC CCGCGGATCC CCCAGGCCAA CCTTCAGCAG 
TTCCCCTACC CTCCCAACTA CGGTACAGGA CTGGCCTCGC CGCCCCCTTC CACCAGCCCT 
TTCTCCCCCG GTTCACCCAA CCTCCCTGCC CCTCAGCTGC TCTCCCACAG CGCCATGCAT 
GGCTCTCAGA TGAGTCTAGC TAACCAGGGG ATGCTGGGCA GTCTGAGCGG ACAGTTTGGG 
GCTGTTATGA GCCCTCAAAT GCAGCACAGT GCCTTTCAGT TCCCCAGCTC AGGTATGAGT 
CAGCAGTCAG ACGGTGGGTT TGGTGGTGCC GCGACCCCTC AGAGCCCCCT CATGTCACCC 
AGGATGGGTC ACGCTCAGAG TCCCATGATG CAACAGGCGC AGGGAAACAG CTCTTTCCAG 
TCGTCTCCTG ATATGAACGG CTGGCCGCAG GGCAACATCA ACACCAACAG TATGTTCACT 
CAGCAGTCGC CACCTCAGTT CAGCCAACAA GCCAACAACA ACATGTACAA CGGCAACGGC 
ATGAACCTCA ACGTGTCCAT GGCCGCCAAC TCCAGCAACA TGGGCCAAAT GGGCAACCAG 
ATGAGCATGA GCTCCATGAA CTCTGGGCCT GGGTCCGGCA TGGCCAACAT GGGCCCTGAA 
CAGCAGAAGT ATTGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_571852.1
CDS	196..4713
Translation	MSAVGENSSDPARSEAQKRKEGPSDLLGPSPKRSTEKRNREHESKYIEELAELIFANFNDIDNFNVKPDKCAILKETVKQIRQIKEQEKAAAANEDEVQKADVSSTGQSVIDKDALGPMMLEALDGFFFVVNMEGNIVFVSENVTQYLRYNQEELMNTSVYSILHVGDHAEFIKNLLPKSHVNGVPWSSENPRRNSHTFNCRMLVNPHSEAEETQDHEAQQKYETMQCFAVSEPKSIKEEGEDFQSCLICVARRVPMKERPMLPTQESFTTRQDLQGKITSLDTSLLRASMKPGWEDLVRRCIQRFHLQNDGDISFAKRHQQEVIRHGQAFSPIYRFSLSDGTIVSAHTKSKLVRSSSTNEPQLYMSLHILQREQPVCGMASDLGNAQTMGKPMNPMSSPNTAGSSCTPQGQDATISSNTSTFPSPGAQKEPGAMHRFGCPGTMSHSATMQAATPQGSGYPLKLSSPSQGSPGMLSPRHRASPGVAGSPRLPPPQFSPAGSLHSPASMCTSSTGGNGGAGANHGYTSSSLNALQALSECHGVSHGQSLGSPDRKLGSPAAHSVAVSHHLINKMSVPESFGEQNQSEQGTPGQDEGIDLPRDEKGNLGQFGNLDGSDPQSRLRDNKSHTKLLQLLTTKTEPIESTSPPPMAGGEPGCKDGGAGNGGMGSQGGNGSHATSLKEKHKILHRLLQNSSSPVDLAKLTAEATGKELCQDSAGGTAGVPELAIKQEPVSPKKKDNALLRYLLDKDDNVLKGKGIKMEPGEIKEEGGKMTGIKTEKTDGGYDRVEPSSELDDILDDLQNSQPGLFTDSRPVSLPSAVDKQSIINDILQMTGESGANMSPQQQRALQTAVSQQNFPAPRPGQPGRAPPVRSVSLDMNMPPKGAQGQYPMMRNNNPYSIMQQQGMMGNHAVMPNQPNMVNAGVMGNNGPRVGMQQDGWGAQGTVGSAASPATAAPAGQHSLPQGALHSRMVPNPATGMPMRPNSQPGPRPMLQPQMMANAQSNMDMGMAGHQFPQQQAPPNQTAPWPDSVMPIEPVPFGNQSSSMYGGSQEDVLCPPASEGPADEGALLSQLYSALKDFDGLEEIDRALGIPALVGQAQPVEQDQFPGQDPSMMMDQKPPMYGQQQYASSPANMQQAGYTPMQDATFHGLQGQMGQRPGYPMLRMQARPGLRPGGVVPNQPNTLRLQLQHRLQAQQNRQPMMNQMGGVSNMNLPLRPNVPNQGTINAQMLAQRQRELLSNHLRQRQLDQQRQQQQQQQQQRSLAMRAQGLTMPPNMAAAAAGMPGAMSNPRIPQANLQQFPYPPNYGTGLASPPPSTSPFSPGSPNLPAPQLLSHSAMHGSQMSLANQGMLGSLSGQFGAVMSPQMQHSAFQFPSSGMSQQSDGGFGGAATPQSPLMSPRMGHAQSPMMQQAQGNSSFQSSPDMNGWPQGNINTNSMFTQQSPPQFSQQANNNMYNGNGMNLNVSMAANSSNMGQMGNQMSMSSMNSGPGSGMANMGPEQQKYC

Target ORF information:

RefSeq Version	NM_131777.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio nuclear receptor coactivator 2 (ncoa2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_131777.1

ORF Insert Sequence:

ATGAGTGCTG TCGGGGAAAA CTCGTCTGAC CCTGCCAGGT CAGAGGCTCA GAAGCGCAAG 
GAAGGCCCAT CAGACCTCCT GGGTCCAAGT CCCAAACGAA GTACAGAAAA ACGTAATCGG 
GAACATGAAA GTAAGTACAT TGAGGAGCTC GCCGAGCTGA TTTTCGCAAA CTTCAATGAC 
ATTGACAACT TCAACGTCAA GCCTGACAAA TGTGCAATCC TAAAGGAAAC CGTGAAACAG 
ATTCGGCAAA TTAAAGAGCA AGAGAAAGCT GCTGCAGCCA ATGAGGATGA AGTGCAGAAG 
GCTGATGTGT CGTCGACAGG CCAAAGTGTT ATTGACAAAG ATGCCCTTGG ACCAATGATG 
CTGGAGGCCT TGGATGGGTT CTTCTTTGTG GTGAACATGG AGGGCAACAT TGTGTTTGTA 
TCGGAGAATG TGACGCAGTA CCTGCGCTAT AATCAGGAAG AGCTGATGAA CACCAGTGTG 
TACAGCATAC TGCATGTAGG AGACCATGCC GAATTCATCA AAAACCTCCT GCCTAAGTCA 
CACGTGAATG GAGTGCCGTG GTCCAGCGAA AACCCTCGGC GGAACAGCCA CACCTTCAAC 
TGCCGCATGC TTGTGAACCC TCACAGCGAG GCCGAAGAGA CGCAGGATCA CGAGGCACAG 
CAGAAATATG AGACTATGCA GTGTTTTGCT GTGTCTGAAC CAAAGTCCAT CAAGGAGGAG 
GGAGAGGATT TCCAGTCGTG TCTGATCTGC GTGGCGCGGC GAGTGCCCAT GAAAGAGAGA 
CCGATGCTCC CCACGCAGGA GAGCTTCACC ACCCGCCAGG ACCTCCAAGG TAAAATCACG 
TCTCTGGACA CAAGTCTGTT ACGAGCGTCT ATGAAGCCAG GCTGGGAAGA CTTGGTGCGG 
AGATGCATTC AGCGTTTTCA TCTTCAGAAC GATGGAGACA TTTCCTTCGC CAAACGACAT 
CAGCAAGAAG TGATAAGGCA CGGGCAGGCG TTCAGTCCCA TCTACCGGTT CTCCCTGTCA 
GACGGGACCA TCGTCTCTGC TCACACCAAG AGCAAACTGG TTCGCTCATC CTCTACCAAC 
GAGCCACAGC TCTACATGTC CCTGCACATC TTACAGAGGG AGCAGCCCGT CTGTGGCATG 
GCCTCTGATC TTGGAAATGC TCAGACCATG GGAAAACCCA TGAACCCCAT GAGCTCTCCA 
AACACAGCGG GTTCCTCGTG CACTCCCCAA GGGCAGGACG CCACCATCAG CAGCAACACA 
TCCACTTTCC CCTCCCCTGG TGCCCAGAAG GAGCCTGGTG CCATGCACCG TTTCGGCTGC 
CCGGGAACCA TGAGCCATTC GGCAACCATG CAAGCGGCCA CCCCTCAGGG CAGCGGGTAT 
CCACTGAAGC TGAGCAGCCC GTCTCAGGGT AGTCCGGGCA TGCTTTCTCC ACGTCACCGA 
GCCAGTCCTG GAGTAGCAGG ATCTCCACGC TTACCCCCGC CACAGTTCTC TCCAGCGGGA 
AGCCTCCATT CACCTGCCAG CATGTGCACT AGCAGTACTG GTGGCAATGG GGGAGCAGGG 
GCCAACCATG GTTACACAAG CAGTTCACTT AATGCTCTTC AGGCGCTTAG CGAATGCCAT 
GGCGTAAGCC ACGGACAATC CCTGGGATCG CCTGATAGAA AGTTAGGGTC CCCAGCTGCA 
CATTCGGTAG CAGTAAGTCA TCACCTAATT AACAAAATGA GCGTACCTGA GTCATTCGGA 
GAGCAGAACC AATCAGAGCA GGGCACCCCT GGACAGGATG AAGGCATTGA TCTGCCAAGA 
GATGAAAAGG GCAACCTCGG GCAGTTCGGC AACCTGGACG GCAGTGATCC TCAAAGCCGA 
CTGCGCGACA ACAAAAGTCA CACTAAACTC CTGCAGCTCC TCACCACTAA AACTGAGCCT 
ATCGAGTCCA CTTCACCCCC TCCAATGGCC GGTGGAGAAC CTGGGTGTAA AGACGGCGGT 
GCAGGGAATG GTGGCATGGG TTCTCAAGGC GGAAACGGGT CACATGCTAC CTCCCTGAAG 
GAGAAACACA AAATCCTTCA CAGACTCCTC CAGAACAGCT CGTCGCCTGT TGATTTGGCC 
AAACTTACAG CTGAGGCCAC AGGGAAAGAG CTGTGTCAGG ATTCTGCTGG CGGGACGGCC 
GGTGTGCCCG AGTTGGCCAT TAAACAAGAG CCTGTCAGTC CCAAGAAAAA GGACAATGCC 
TTGCTGCGCT ACCTACTGGA TAAAGATGAC AATGTACTCA AGGGTAAAGG TATTAAAATG 
GAGCCTGGAG AGATCAAGGA GGAGGGGGGC AAGATGACTG GGATTAAGAC CGAGAAAACT 
GATGGTGGCT ACGACCGTGT GGAGCCGTCA AGTGAGCTGG ATGATATCCT GGATGATCTT 
CAGAACAGTC AGCCGGGCCT GTTCACTGAC TCCAGGCCTG TGTCTCTGCC TTCTGCTGTG 
GACAAGCAGT CAATCATCAA TGACATCCTG CAGATGACAG GAGAGAGCGG AGCTAACATG 
AGTCCTCAAC AGCAGAGAGC ACTGCAGACT GCAGTCAGCC AACAGAATTT CCCTGCTCCA 
CGGCCCGGTC AGCCTGGCAG AGCTCCTCCG GTCAGAAGCG TGTCTCTGGA CATGAACATG 
CCTCCTAAAG GCGCTCAGGG TCAGTATCCT ATGATGAGGA ACAACAACCC CTACTCAATC 
ATGCAGCAGC AAGGCATGAT GGGAAACCAC GCCGTCATGC CCAATCAGCC CAACATGGTG 
AACGCAGGAG TCATGGGTAA TAATGGTCCC CGTGTAGGCA TGCAGCAGGA CGGTTGGGGT 
GCTCAGGGTA CCGTCGGCAG CGCAGCTTCA CCTGCCACAG CTGCTCCCGC TGGCCAGCAC 
TCTCTCCCGC AGGGGGCACT GCACAGCCGC ATGGTGCCCA ACCCTGCCAC AGGAATGCCC 
ATGAGACCCA ACAGCCAGCC TGGACCCAGA CCAATGCTGC AACCGCAGAT GATGGCCAAT 
GCTCAGTCAA ACATGGACAT GGGCATGGCT GGCCACCAGT TTCCCCAGCA ACAGGCACCG 
CCCAATCAGA CGGCTCCATG GCCAGACAGT GTGATGCCTA TAGAGCCGGT GCCGTTTGGG 
AACCAGAGCA GTTCCATGTA CGGCGGGTCT CAGGAGGATG TGTTGTGCCC TCCTGCCTCT 
GAAGGTCCGG CTGATGAGGG GGCTTTACTC AGCCAGCTCT ACTCGGCCCT CAAAGATTTT 
GATGGTCTGG AGGAGATTGA CCGTGCGCTT GGCATTCCAG CGCTAGTGGG ACAGGCTCAG 
CCTGTGGAGC AGGATCAGTT CCCTGGTCAG GACCCTAGTA TGATGATGGA TCAGAAGCCA 
CCGATGTACG GGCAGCAACA GTATGCCTCG TCCCCGGCAA ACATGCAGCA GGCTGGATAC 
ACGCCAATGC AAGATGCCAC TTTCCACGGC TTGCAAGGAC AGATGGGACA GAGACCCGGA 
TACCCAATGC TCCGAATGCA AGCGAGACCG GGACTCAGAC CCGGTGGAGT CGTCCCAAAT 
CAACCCAATA CACTCAGACT GCAGCTTCAG CACAGGCTAC AAGCACAGCA GAATCGTCAG 
CCTATGATGA ATCAGATGGG TGGAGTGTCA AATATGAACC TACCTCTCAG ACCCAACGTT 
CCAAATCAGG GGACAATCAA CGCTCAGATG CTGGCCCAGC GGCAGAGAGA GTTGCTGAGT 
AATCATTTAC GTCAGCGGCA GCTCGACCAG CAGCGGCAGC AACAGCAACA GCAGCAGCAG 
CAGCGCAGTC TGGCCATGCG GGCGCAGGGC CTCACCATGC CACCCAACAT GGCGGCAGCC 
GCAGCGGGCA TGCCAGGAGC CATGAGCAAC CCGCGGATCC CCCAGGCCAA CCTTCAGCAG 
TTCCCCTACC CTCCCAACTA CGGTACAGGA CTGGCCTCGC CGCCCCCTTC CACCAGCCCT 
TTCTCCCCCG GTTCACCCAA CCTCCCTGCC CCTCAGCTGC TCTCCCACAG CGCCATGCAT 
GGCTCTCAGA TGAGTCTAGC TAACCAGGGG ATGCTGGGCA GTCTGAGCGG ACAGTTTGGG 
GCTGTTATGA GCCCTCAAAT GCAGCACAGT GCCTTTCAGT TCCCCAGCTC AGGTATGAGT 
CAGCAGTCAG ACGGTGGGTT TGGTGGTGCC GCGACCCCTC AGAGCCCCCT CATGTCACCC 
AGGATGGGTC ACGCTCAGAG TCCCATGATG CAACAGGCGC AGGGAAACAG CTCTTTCCAG 
TCGTCTCCTG ATATGAACGG CTGGCCGCAG GGCAACATCA ACACCAACAG TATGTTCACT 
CAGCAGTCGC CACCTCAGTT CAGCCAACAA GCCAACAACA ACATGTACAA CGGCAACGGC 
ATGAACCTCA ACGTGTCCAT GGCCGCCAAC TCCAGCAACA TGGGCCAAAT GGGCAACCAG 
ATGAGCATGA GCTCCATGAA CTCTGGGCCT GGGTCCGGCA TGGCCAACAT GGGCCCTGAA 
CAGCAGAAGT ATTGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ncoa2 ( XM_009297336.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009295611.1 Danio rerio nuclear receptor coactivator 2 (ncoa2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa39440
Clone ID Related Accession (Same CDS sequence)	XM_009297336.2 , XM_009297336.3
Accession Version	XM_009297336.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4518bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	nuclear receptor coactivator 2 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007135.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009297336.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGCTG TCGGGGAAAA CTCGTCTGAC CCTGCCAGGT CAGAGGCTCA GAAGCGCAAG 
GAAGGCCCAT CAGACCTCCT GGGTCCAAGT CCCAAACGAA GTACAGAAAA ACGTAACCGG 
GAACATGAAA ATAAGTACAT TGAGGAGCTC GCCGAGCTGA TTTTCGCAAA CTTCAATGAC 
ATTGACAACT TCAACGTCAA GCCTGACAAA TGTGCAATCC TAAAGGAAAC CGTGAAACAG 
ATTCGGCAAA TTAAAGAGCA AGAGAAAGCT GCTGCAGCCA ATGAGGATGA AGTGCAGAAG 
GCTGATGTGT CGTCGACAGG CCAAAGTGTT ATTGACAAAG ATGCCCTTGG ACCAATGATG 
CTGGAGGCCT TGGATGGGTT CTTCTTTGTG GTGAACATGG AGGGCAACAT TGTGTTTGTA 
TCGGAGAATG TGACGCAGTA CCTGCGCTAT AATCAGGAAG AGCTGATGAA CACCAGTGTG 
TACAGCATAC TGCATGTAGG AGACCATGCC GAATTCATCA AAAACCTCCT GCCTAAGTCA 
CACGTGAATG GAGTGCCGTG GTCCAGCGAA AACCCTCGGC GGAACAGCCA CACCTTCAAC 
TGCCGCATGC TTGTGAACCC TCACAGCGAG GCCGAAGAGA CGCAGGATCA CGAGGCACAG 
CAGAAATATG AGACTATGCA GTGTTTTGCT GTGTCTGAAC CAAAGTCCAT CAAGGAGGAG 
GGAGAGGATT TCCAGTCGTG TCTGATCTGC GTGGCGCGGC GAGTGCCCAT GAAAGAGAGA 
CCGATGCTCC CCACGCAGGA GAGCTTCACC ACCCGCCAGG ACCTCCAAGG TAAAATCACG 
TCTCTGGACA CAAGTCTGTT ACGAGCGTCT ATGAAGCCAG GCTGGGAAGA CTTGGTGCGG 
AGATGCATTC AGCGTTTTCA TCTTCAGAAC GATGGAGACA TTTCCTTCGC CAAACGACAT 
CAGCAAGAAG TGATAAGGCA CGGGCAGGCG TTCAGTCCCA TCTACCGGTT CTCCCTGTCA 
GACGGGACCA TCGTCTCTGC TCACACCAAG AGCAAACTGG TTCGCTCATC CTCTACCAAC 
GAGCCACAGC TCTACATGTC CCTGCACATC TTACAGAGGG AGCAGCCCGT CTGTGGCATG 
GCCTCTGATC TTGGAAATGC TCAGACCATG GGAAAACCCA TGAACCCCAT GAGCTCTCCA 
AACACAGCGG GTTCCTCGTG CACTCCCCAA GGGCAGGACG CCACCATCAG CAGCAACACA 
TCCACTTTCC CCTCCCCTGG TGCCCAGAAG GAGCCTGGTG CCATGCACCG TTTCGGCTGC 
CCGGGAACCA TGAGCCATTC GGCAACCATG CAAGCGGCCA CCCCTCAGGG CAGCGGGTAT 
CCACTGAAGC TGAGCAGCCC GTCTCAGGGT AGTCCGGGCA TGCTTTCTCC ACGTCACCGA 
GCCAGTCCTG GAGTAGCAGG ATCTCCACGC TTACCCCCGC CACAGTTCTC TCCAGCGGGA 
AGCCTCCATT CACCTGCCAG CATGTGCACT AGCAGTACTG GTGGCAATGG GGGAGCAGGG 
GCCAACCATG GTTACACAAG CAGTTCACTT AATGCTCTTC AGGCGCTTAG CGAATGCCAT 
GGCGTAAGCC ACGGACAATC CCTGGGATCG CCTGATAGAA AGTTAGGGTC CCCAGCTGCA 
CATTCGGTAG CAGTAAGTCA TCACCTAATT AACAAAATGA GCGTACCTGA GTCATTCGGA 
GAGCAGAACC AATCAGAGCA GGGCACCCCT GGACAGGATG AAGGCATTGA TCTGCCAAGA 
GATGAAAAGG GCAACCTCGG GCAGTTCGGC AACCTGGACG GCAGTGATCC TCAAAGCCGA 
CTGCGCGACA ACAAAAGTCA CACTAAACTC CTGCAGCTCC TCACCACTAA AACTGAGCCT 
ATCGAGTCCA CTTCACCCCC TCCAATGGCC GGTGGAGAAC CTGGGTGTAA AGACGGCGGT 
GCAGGGAATG GTGGCATGGG TTCTCAAGGC GGAAACGGGT CACATGCTAC CTCCCTGAAG 
GAGAAACACA AAATCCTTCA CAGACTCCTC CAGAACAGCT CTTCGCCTGT TGATTTGGCC 
AAACTTACAG CTGAGGCCAC AGGGAAAGAG CTGTGTCAGG ATTCTGCTGG CGGGACGGCC 
GGTGTGCCCG AGTTGGCCAT TAAACAAGAG CCTGTCAGTC CCAAGAAAAA GGACAATGCC 
TTGCTGCGCT ACCTACTGGA TAAAGATGAC AATGTACTCA AGGGTAAAGG TATTAAAATG 
GAGCCTGGAG AGATCAAGGA GGAGGGGGGC AAGATGACTG GGATTAAGAC CGAGAAAACT 
GATGGTGGCT ACGACCGTGT GGAGCCGTCA AGTGAGCTGG ATGATATCCT GGATGATCTT 
CAGAACAGTC AGCCGGGCCT GTTCACTGAC TCCAGGCCTG TGTCTCTGCC TTCTGCTGTG 
GACAAGCAGT CAATCATCAA TGACATCCTG CAGATGACAG GAGAGAGCGG AGCTAACATG 
AGTCCTCAAC AGCAGAGAGC ACTGCAGACT GCAGTCAGCC AACAGAATTT CCCTGCTCCA 
CGGCCCGGTC AGCCTGGCAG AGCTCCTCCG GTCAGAAGCG TGTCTCTGGA CATGAACATG 
CCTCCTAAAG GCGCTCAGGG TCAGTATCCT ATGATGAGGA ACAACAACCC CTACTCAATC 
ATGCAGCAGC AAGGCATGAT GGGAAACCAC GCCGTCATGC CCAATCAGCC CAACATGGTG 
AACGCAGGAG TCATGGGTAA TAATGGTCCC CGTGTAGGCA TGCAGCAGGA CGGTTGGGGT 
GCTCAGGGTA CCGTCGGCAG CGCAGCTTCA CCTGCCACAG CTGCTCCCGC TGGCCAGCAC 
TCTCTCCCGC AGGGGGCACT GCACAGCCGC ATGGTGCCCA ACCCTGCCAC AGGAATGCCC 
ATGAGACCCA ACAGCCAGCC TGGACCCAGA CCAATGCTGC AACCGCAGAT GATGGCCAAT 
GCTCAGTCAA ACATGGACAT GGGCATGGCT GGCCACCAGT TTCCCCAGCA ACAGGCACCG 
CCCAATCAGA CGGCTCCATG GCCAGACAGT GTGATGCCTA TAGAGCCGGT GCCGTTTGGG 
AACCAGAGCA GTTCCATGTA CGGCGGGTCT CAGGAGGATG TGTTGTGCCC TCCTGCCTCT 
GAAGGTCCGG CTGATGAGGG GGCTTTACTC AGCCAGCTCT ACTCGGCCCT CAAAGATTTT 
GATGGTCTGG AGGAGATTGA CCGTGCGCTT GGCATTCCAG CGCTAGTGGG ACAGGCTCAG 
CCTGTGGAGC AGGATCAGTT CCCTGGTCAG GACCCTAGTA TGATGATGGA TCAGAAGCCA 
CCGATGTACG GGCAGCAACA GTATGCCTCG TCCCCGGCAA ACATGCAGCA GGCTGGATAC 
ACGCCAATGC AAGATGCCAC TTTCCACGGC TTGCAAGGAC AGATGGGACA GAGACCCGGA 
TACCCAATGC TCCGAATGCA AGCGAGACCG GGACTCAGAC CCGGTGGAGT CGTCCCAAAT 
CAACCCAATA CACTCAGACT GCAGCTTCAG CACAGGCTAC AAGCACAGCA GAATCGTCAG 
CCTATGATGA ATCAGATGGG TGGAGTGTCA AATATGAACC TACCTCTCAG ACCCAACGTT 
CCAAATCAGG GGACAATCAA CGCTCAGATG CTGGCCCAGC GGCAGAGAGA GTTGCTGAGT 
AATCATTTAC GTCAGCGGCA GCTCGACCAG CAGCGGCAGC AACAGCAACA GCAGCAGCAG 
CAGCGCAGTC TGGCCATGCG GGCGCAGGGC CTCACCATGC CACCCAACAT GGCGGCAGCC 
GCAGCGGGCA TGCCAGGAGC CATGAGCAAC CCGCGGATCC CCCAGGCCAA CCCTCAGCAG 
TTCCCCTACC CTCCCAACTA CGGTACAGGA CTGGCCTCGC CGCCCCCTTC CACCAGCCCT 
TTCTCCCCCG GTTCACCCAA CCTCCCTGCC CCTCAGCTGC TCTCCCACAG CGCCATGCAT 
GGCTCTCAGA TGAGTCTAGC TAACCAGGGG ATGCTGGGCA GTCTGAGCGG ACAGTTTGGG 
GCTGTTATGA GCCCTCAAAT GCAGCACAGT GCCTTTCAGT TCCCCAGCTC AGGTATGAGT 
CAGCAGTCAG ACGGTGGGTT TGGTGGTGCC GCGACCCCTC AGAGCCCCCT CATGTCACCC 
AGGATGGGTC ACGCTCAGAG TCCCATGATG CAACAGGCGC AGGGAAACAG CTCTTTCCAG 
TCGTCTCCTG ATATGAACGG CTGGCCGCAG GGCAACATCA ACACCAACAG TATGTTTACT 
CAGCAGTCGC CACCTCAGTT CAGCCAACAA GCCAACAACA ACATGTACAA CGGCAACGGC 
ATGAACCTCA ACGTGTCCAT GGCCGCCAAC TCCAGCAACA TGGGCCAAAT GGGCAACCAG 
ATGAGCATGA GCTCCATGAA CTCTGGGCCT GGGTCCGGCA TGGCCAACAT GGGCCCTGAA 
CAGCAGAAGT ATTGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009295611.1
CDS	403..4920
Translation	MSAVGENSSDPARSEAQKRKEGPSDLLGPSPKRSTEKRNREHENKYIEELAELIFANFNDIDNFNVKPDKCAILKETVKQIRQIKEQEKAAAANEDEVQKADVSSTGQSVIDKDALGPMMLEALDGFFFVVNMEGNIVFVSENVTQYLRYNQEELMNTSVYSILHVGDHAEFIKNLLPKSHVNGVPWSSENPRRNSHTFNCRMLVNPHSEAEETQDHEAQQKYETMQCFAVSEPKSIKEEGEDFQSCLICVARRVPMKERPMLPTQESFTTRQDLQGKITSLDTSLLRASMKPGWEDLVRRCIQRFHLQNDGDISFAKRHQQEVIRHGQAFSPIYRFSLSDGTIVSAHTKSKLVRSSSTNEPQLYMSLHILQREQPVCGMASDLGNAQTMGKPMNPMSSPNTAGSSCTPQGQDATISSNTSTFPSPGAQKEPGAMHRFGCPGTMSHSATMQAATPQGSGYPLKLSSPSQGSPGMLSPRHRASPGVAGSPRLPPPQFSPAGSLHSPASMCTSSTGGNGGAGANHGYTSSSLNALQALSECHGVSHGQSLGSPDRKLGSPAAHSVAVSHHLINKMSVPESFGEQNQSEQGTPGQDEGIDLPRDEKGNLGQFGNLDGSDPQSRLRDNKSHTKLLQLLTTKTEPIESTSPPPMAGGEPGCKDGGAGNGGMGSQGGNGSHATSLKEKHKILHRLLQNSSSPVDLAKLTAEATGKELCQDSAGGTAGVPELAIKQEPVSPKKKDNALLRYLLDKDDNVLKGKGIKMEPGEIKEEGGKMTGIKTEKTDGGYDRVEPSSELDDILDDLQNSQPGLFTDSRPVSLPSAVDKQSIINDILQMTGESGANMSPQQQRALQTAVSQQNFPAPRPGQPGRAPPVRSVSLDMNMPPKGAQGQYPMMRNNNPYSIMQQQGMMGNHAVMPNQPNMVNAGVMGNNGPRVGMQQDGWGAQGTVGSAASPATAAPAGQHSLPQGALHSRMVPNPATGMPMRPNSQPGPRPMLQPQMMANAQSNMDMGMAGHQFPQQQAPPNQTAPWPDSVMPIEPVPFGNQSSSMYGGSQEDVLCPPASEGPADEGALLSQLYSALKDFDGLEEIDRALGIPALVGQAQPVEQDQFPGQDPSMMMDQKPPMYGQQQYASSPANMQQAGYTPMQDATFHGLQGQMGQRPGYPMLRMQARPGLRPGGVVPNQPNTLRLQLQHRLQAQQNRQPMMNQMGGVSNMNLPLRPNVPNQGTINAQMLAQRQRELLSNHLRQRQLDQQRQQQQQQQQQRSLAMRAQGLTMPPNMAAAAAGMPGAMSNPRIPQANPQQFPYPPNYGTGLASPPPSTSPFSPGSPNLPAPQLLSHSAMHGSQMSLANQGMLGSLSGQFGAVMSPQMQHSAFQFPSSGMSQQSDGGFGGAATPQSPLMSPRMGHAQSPMMQQAQGNSSFQSSPDMNGWPQGNINTNSMFTQQSPPQFSQQANNNMYNGNGMNLNVSMAANSSNMGQMGNQMSMSSMNSGPGSGMANMGPEQQKYC

Target ORF information:

RefSeq Version	XM_009297336.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio nuclear receptor coactivator 2 (ncoa2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009297336.2

ORF Insert Sequence:

ATGAGTGCTG TCGGGGAAAA CTCGTCTGAC CCTGCCAGGT CAGAGGCTCA GAAGCGCAAG 
GAAGGCCCAT CAGACCTCCT GGGTCCAAGT CCCAAACGAA GTACAGAAAA ACGTAACCGG 
GAACATGAAA ATAAGTACAT TGAGGAGCTC GCCGAGCTGA TTTTCGCAAA CTTCAATGAC 
ATTGACAACT TCAACGTCAA GCCTGACAAA TGTGCAATCC TAAAGGAAAC CGTGAAACAG 
ATTCGGCAAA TTAAAGAGCA AGAGAAAGCT GCTGCAGCCA ATGAGGATGA AGTGCAGAAG 
GCTGATGTGT CGTCGACAGG CCAAAGTGTT ATTGACAAAG ATGCCCTTGG ACCAATGATG 
CTGGAGGCCT TGGATGGGTT CTTCTTTGTG GTGAACATGG AGGGCAACAT TGTGTTTGTA 
TCGGAGAATG TGACGCAGTA CCTGCGCTAT AATCAGGAAG AGCTGATGAA CACCAGTGTG 
TACAGCATAC TGCATGTAGG AGACCATGCC GAATTCATCA AAAACCTCCT GCCTAAGTCA 
CACGTGAATG GAGTGCCGTG GTCCAGCGAA AACCCTCGGC GGAACAGCCA CACCTTCAAC 
TGCCGCATGC TTGTGAACCC TCACAGCGAG GCCGAAGAGA CGCAGGATCA CGAGGCACAG 
CAGAAATATG AGACTATGCA GTGTTTTGCT GTGTCTGAAC CAAAGTCCAT CAAGGAGGAG 
GGAGAGGATT TCCAGTCGTG TCTGATCTGC GTGGCGCGGC GAGTGCCCAT GAAAGAGAGA 
CCGATGCTCC CCACGCAGGA GAGCTTCACC ACCCGCCAGG ACCTCCAAGG TAAAATCACG 
TCTCTGGACA CAAGTCTGTT ACGAGCGTCT ATGAAGCCAG GCTGGGAAGA CTTGGTGCGG 
AGATGCATTC AGCGTTTTCA TCTTCAGAAC GATGGAGACA TTTCCTTCGC CAAACGACAT 
CAGCAAGAAG TGATAAGGCA CGGGCAGGCG TTCAGTCCCA TCTACCGGTT CTCCCTGTCA 
GACGGGACCA TCGTCTCTGC TCACACCAAG AGCAAACTGG TTCGCTCATC CTCTACCAAC 
GAGCCACAGC TCTACATGTC CCTGCACATC TTACAGAGGG AGCAGCCCGT CTGTGGCATG 
GCCTCTGATC TTGGAAATGC TCAGACCATG GGAAAACCCA TGAACCCCAT GAGCTCTCCA 
AACACAGCGG GTTCCTCGTG CACTCCCCAA GGGCAGGACG CCACCATCAG CAGCAACACA 
TCCACTTTCC CCTCCCCTGG TGCCCAGAAG GAGCCTGGTG CCATGCACCG TTTCGGCTGC 
CCGGGAACCA TGAGCCATTC GGCAACCATG CAAGCGGCCA CCCCTCAGGG CAGCGGGTAT 
CCACTGAAGC TGAGCAGCCC GTCTCAGGGT AGTCCGGGCA TGCTTTCTCC ACGTCACCGA 
GCCAGTCCTG GAGTAGCAGG ATCTCCACGC TTACCCCCGC CACAGTTCTC TCCAGCGGGA 
AGCCTCCATT CACCTGCCAG CATGTGCACT AGCAGTACTG GTGGCAATGG GGGAGCAGGG 
GCCAACCATG GTTACACAAG CAGTTCACTT AATGCTCTTC AGGCGCTTAG CGAATGCCAT 
GGCGTAAGCC ACGGACAATC CCTGGGATCG CCTGATAGAA AGTTAGGGTC CCCAGCTGCA 
CATTCGGTAG CAGTAAGTCA TCACCTAATT AACAAAATGA GCGTACCTGA GTCATTCGGA 
GAGCAGAACC AATCAGAGCA GGGCACCCCT GGACAGGATG AAGGCATTGA TCTGCCAAGA 
GATGAAAAGG GCAACCTCGG GCAGTTCGGC AACCTGGACG GCAGTGATCC TCAAAGCCGA 
CTGCGCGACA ACAAAAGTCA CACTAAACTC CTGCAGCTCC TCACCACTAA AACTGAGCCT 
ATCGAGTCCA CTTCACCCCC TCCAATGGCC GGTGGAGAAC CTGGGTGTAA AGACGGCGGT 
GCAGGGAATG GTGGCATGGG TTCTCAAGGC GGAAACGGGT CACATGCTAC CTCCCTGAAG 
GAGAAACACA AAATCCTTCA CAGACTCCTC CAGAACAGCT CTTCGCCTGT TGATTTGGCC 
AAACTTACAG CTGAGGCCAC AGGGAAAGAG CTGTGTCAGG ATTCTGCTGG CGGGACGGCC 
GGTGTGCCCG AGTTGGCCAT TAAACAAGAG CCTGTCAGTC CCAAGAAAAA GGACAATGCC 
TTGCTGCGCT ACCTACTGGA TAAAGATGAC AATGTACTCA AGGGTAAAGG TATTAAAATG 
GAGCCTGGAG AGATCAAGGA GGAGGGGGGC AAGATGACTG GGATTAAGAC CGAGAAAACT 
GATGGTGGCT ACGACCGTGT GGAGCCGTCA AGTGAGCTGG ATGATATCCT GGATGATCTT 
CAGAACAGTC AGCCGGGCCT GTTCACTGAC TCCAGGCCTG TGTCTCTGCC TTCTGCTGTG 
GACAAGCAGT CAATCATCAA TGACATCCTG CAGATGACAG GAGAGAGCGG AGCTAACATG 
AGTCCTCAAC AGCAGAGAGC ACTGCAGACT GCAGTCAGCC AACAGAATTT CCCTGCTCCA 
CGGCCCGGTC AGCCTGGCAG AGCTCCTCCG GTCAGAAGCG TGTCTCTGGA CATGAACATG 
CCTCCTAAAG GCGCTCAGGG TCAGTATCCT ATGATGAGGA ACAACAACCC CTACTCAATC 
ATGCAGCAGC AAGGCATGAT GGGAAACCAC GCCGTCATGC CCAATCAGCC CAACATGGTG 
AACGCAGGAG TCATGGGTAA TAATGGTCCC CGTGTAGGCA TGCAGCAGGA CGGTTGGGGT 
GCTCAGGGTA CCGTCGGCAG CGCAGCTTCA CCTGCCACAG CTGCTCCCGC TGGCCAGCAC 
TCTCTCCCGC AGGGGGCACT GCACAGCCGC ATGGTGCCCA ACCCTGCCAC AGGAATGCCC 
ATGAGACCCA ACAGCCAGCC TGGACCCAGA CCAATGCTGC AACCGCAGAT GATGGCCAAT 
GCTCAGTCAA ACATGGACAT GGGCATGGCT GGCCACCAGT TTCCCCAGCA ACAGGCACCG 
CCCAATCAGA CGGCTCCATG GCCAGACAGT GTGATGCCTA TAGAGCCGGT GCCGTTTGGG 
AACCAGAGCA GTTCCATGTA CGGCGGGTCT CAGGAGGATG TGTTGTGCCC TCCTGCCTCT 
GAAGGTCCGG CTGATGAGGG GGCTTTACTC AGCCAGCTCT ACTCGGCCCT CAAAGATTTT 
GATGGTCTGG AGGAGATTGA CCGTGCGCTT GGCATTCCAG CGCTAGTGGG ACAGGCTCAG 
CCTGTGGAGC AGGATCAGTT CCCTGGTCAG GACCCTAGTA TGATGATGGA TCAGAAGCCA 
CCGATGTACG GGCAGCAACA GTATGCCTCG TCCCCGGCAA ACATGCAGCA GGCTGGATAC 
ACGCCAATGC AAGATGCCAC TTTCCACGGC TTGCAAGGAC AGATGGGACA GAGACCCGGA 
TACCCAATGC TCCGAATGCA AGCGAGACCG GGACTCAGAC CCGGTGGAGT CGTCCCAAAT 
CAACCCAATA CACTCAGACT GCAGCTTCAG CACAGGCTAC AAGCACAGCA GAATCGTCAG 
CCTATGATGA ATCAGATGGG TGGAGTGTCA AATATGAACC TACCTCTCAG ACCCAACGTT 
CCAAATCAGG GGACAATCAA CGCTCAGATG CTGGCCCAGC GGCAGAGAGA GTTGCTGAGT 
AATCATTTAC GTCAGCGGCA GCTCGACCAG CAGCGGCAGC AACAGCAACA GCAGCAGCAG 
CAGCGCAGTC TGGCCATGCG GGCGCAGGGC CTCACCATGC CACCCAACAT GGCGGCAGCC 
GCAGCGGGCA TGCCAGGAGC CATGAGCAAC CCGCGGATCC CCCAGGCCAA CCCTCAGCAG 
TTCCCCTACC CTCCCAACTA CGGTACAGGA CTGGCCTCGC CGCCCCCTTC CACCAGCCCT 
TTCTCCCCCG GTTCACCCAA CCTCCCTGCC CCTCAGCTGC TCTCCCACAG CGCCATGCAT 
GGCTCTCAGA TGAGTCTAGC TAACCAGGGG ATGCTGGGCA GTCTGAGCGG ACAGTTTGGG 
GCTGTTATGA GCCCTCAAAT GCAGCACAGT GCCTTTCAGT TCCCCAGCTC AGGTATGAGT 
CAGCAGTCAG ACGGTGGGTT TGGTGGTGCC GCGACCCCTC AGAGCCCCCT CATGTCACCC 
AGGATGGGTC ACGCTCAGAG TCCCATGATG CAACAGGCGC AGGGAAACAG CTCTTTCCAG 
TCGTCTCCTG ATATGAACGG CTGGCCGCAG GGCAACATCA ACACCAACAG TATGTTTACT 
CAGCAGTCGC CACCTCAGTT CAGCCAACAA GCCAACAACA ACATGTACAA CGGCAACGGC 
ATGAACCTCA ACGTGTCCAT GGCCGCCAAC TCCAGCAACA TGGGCCAAAT GGGCAACCAG 
ATGAGCATGA GCTCCATGAA CTCTGGGCCT GGGTCCGGCA TGGCCAACAT GGGCCCTGAA 
CAGCAGAAGT ATTGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ncoa2 ( XM_009297336.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009295611.1 Danio rerio nuclear receptor coactivator 2 (ncoa2), transcript variant X1, mRNA.

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CloneID	ODa39440
Clone ID Related Accession (Same CDS sequence)	XM_009297336.2 , XM_009297336.3
Accession Version	XM_009297336.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4518bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	nuclear receptor coactivator 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007135.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009297336.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGCTG TCGGGGAAAA CTCGTCTGAC CCTGCCAGGT CAGAGGCTCA GAAGCGCAAG 
GAAGGCCCAT CAGACCTCCT GGGTCCAAGT CCCAAACGAA GTACAGAAAA ACGTAACCGG 
GAACATGAAA ATAAGTACAT TGAGGAGCTC GCCGAGCTGA TTTTCGCAAA CTTCAATGAC 
ATTGACAACT TCAACGTCAA GCCTGACAAA TGTGCAATCC TAAAGGAAAC CGTGAAACAG 
ATTCGGCAAA TTAAAGAGCA AGAGAAAGCT GCTGCAGCCA ATGAGGATGA AGTGCAGAAG 
GCTGATGTGT CGTCGACAGG CCAAAGTGTT ATTGACAAAG ATGCCCTTGG ACCAATGATG 
CTGGAGGCCT TGGATGGGTT CTTCTTTGTG GTGAACATGG AGGGCAACAT TGTGTTTGTA 
TCGGAGAATG TGACGCAGTA CCTGCGCTAT AATCAGGAAG AGCTGATGAA CACCAGTGTG 
TACAGCATAC TGCATGTAGG AGACCATGCC GAATTCATCA AAAACCTCCT GCCTAAGTCA 
CACGTGAATG GAGTGCCGTG GTCCAGCGAA AACCCTCGGC GGAACAGCCA CACCTTCAAC 
TGCCGCATGC TTGTGAACCC TCACAGCGAG GCCGAAGAGA CGCAGGATCA CGAGGCACAG 
CAGAAATATG AGACTATGCA GTGTTTTGCT GTGTCTGAAC CAAAGTCCAT CAAGGAGGAG 
GGAGAGGATT TCCAGTCGTG TCTGATCTGC GTGGCGCGGC GAGTGCCCAT GAAAGAGAGA 
CCGATGCTCC CCACGCAGGA GAGCTTCACC ACCCGCCAGG ACCTCCAAGG TAAAATCACG 
TCTCTGGACA CAAGTCTGTT ACGAGCGTCT ATGAAGCCAG GCTGGGAAGA CTTGGTGCGG 
AGATGCATTC AGCGTTTTCA TCTTCAGAAC GATGGAGACA TTTCCTTCGC CAAACGACAT 
CAGCAAGAAG TGATAAGGCA CGGGCAGGCG TTCAGTCCCA TCTACCGGTT CTCCCTGTCA 
GACGGGACCA TCGTCTCTGC TCACACCAAG AGCAAACTGG TTCGCTCATC CTCTACCAAC 
GAGCCACAGC TCTACATGTC CCTGCACATC TTACAGAGGG AGCAGCCCGT CTGTGGCATG 
GCCTCTGATC TTGGAAATGC TCAGACCATG GGAAAACCCA TGAACCCCAT GAGCTCTCCA 
AACACAGCGG GTTCCTCGTG CACTCCCCAA GGGCAGGACG CCACCATCAG CAGCAACACA 
TCCACTTTCC CCTCCCCTGG TGCCCAGAAG GAGCCTGGTG CCATGCACCG TTTCGGCTGC 
CCGGGAACCA TGAGCCATTC GGCAACCATG CAAGCGGCCA CCCCTCAGGG CAGCGGGTAT 
CCACTGAAGC TGAGCAGCCC GTCTCAGGGT AGTCCGGGCA TGCTTTCTCC ACGTCACCGA 
GCCAGTCCTG GAGTAGCAGG ATCTCCACGC TTACCCCCGC CACAGTTCTC TCCAGCGGGA 
AGCCTCCATT CACCTGCCAG CATGTGCACT AGCAGTACTG GTGGCAATGG GGGAGCAGGG 
GCCAACCATG GTTACACAAG CAGTTCACTT AATGCTCTTC AGGCGCTTAG CGAATGCCAT 
GGCGTAAGCC ACGGACAATC CCTGGGATCG CCTGATAGAA AGTTAGGGTC CCCAGCTGCA 
CATTCGGTAG CAGTAAGTCA TCACCTAATT AACAAAATGA GCGTACCTGA GTCATTCGGA 
GAGCAGAACC AATCAGAGCA GGGCACCCCT GGACAGGATG AAGGCATTGA TCTGCCAAGA 
GATGAAAAGG GCAACCTCGG GCAGTTCGGC AACCTGGACG GCAGTGATCC TCAAAGCCGA 
CTGCGCGACA ACAAAAGTCA CACTAAACTC CTGCAGCTCC TCACCACTAA AACTGAGCCT 
ATCGAGTCCA CTTCACCCCC TCCAATGGCC GGTGGAGAAC CTGGGTGTAA AGACGGCGGT 
GCAGGGAATG GTGGCATGGG TTCTCAAGGC GGAAACGGGT CACATGCTAC CTCCCTGAAG 
GAGAAACACA AAATCCTTCA CAGACTCCTC CAGAACAGCT CTTCGCCTGT TGATTTGGCC 
AAACTTACAG CTGAGGCCAC AGGGAAAGAG CTGTGTCAGG ATTCTGCTGG CGGGACGGCC 
GGTGTGCCCG AGTTGGCCAT TAAACAAGAG CCTGTCAGTC CCAAGAAAAA GGACAATGCC 
TTGCTGCGCT ACCTACTGGA TAAAGATGAC AATGTACTCA AGGGTAAAGG TATTAAAATG 
GAGCCTGGAG AGATCAAGGA GGAGGGGGGC AAGATGACTG GGATTAAGAC CGAGAAAACT 
GATGGTGGCT ACGACCGTGT GGAGCCGTCA AGTGAGCTGG ATGATATCCT GGATGATCTT 
CAGAACAGTC AGCCGGGCCT GTTCACTGAC TCCAGGCCTG TGTCTCTGCC TTCTGCTGTG 
GACAAGCAGT CAATCATCAA TGACATCCTG CAGATGACAG GAGAGAGCGG AGCTAACATG 
AGTCCTCAAC AGCAGAGAGC ACTGCAGACT GCAGTCAGCC AACAGAATTT CCCTGCTCCA 
CGGCCCGGTC AGCCTGGCAG AGCTCCTCCG GTCAGAAGCG TGTCTCTGGA CATGAACATG 
CCTCCTAAAG GCGCTCAGGG TCAGTATCCT ATGATGAGGA ACAACAACCC CTACTCAATC 
ATGCAGCAGC AAGGCATGAT GGGAAACCAC GCCGTCATGC CCAATCAGCC CAACATGGTG 
AACGCAGGAG TCATGGGTAA TAATGGTCCC CGTGTAGGCA TGCAGCAGGA CGGTTGGGGT 
GCTCAGGGTA CCGTCGGCAG CGCAGCTTCA CCTGCCACAG CTGCTCCCGC TGGCCAGCAC 
TCTCTCCCGC AGGGGGCACT GCACAGCCGC ATGGTGCCCA ACCCTGCCAC AGGAATGCCC 
ATGAGACCCA ACAGCCAGCC TGGACCCAGA CCAATGCTGC AACCGCAGAT GATGGCCAAT 
GCTCAGTCAA ACATGGACAT GGGCATGGCT GGCCACCAGT TTCCCCAGCA ACAGGCACCG 
CCCAATCAGA CGGCTCCATG GCCAGACAGT GTGATGCCTA TAGAGCCGGT GCCGTTTGGG 
AACCAGAGCA GTTCCATGTA CGGCGGGTCT CAGGAGGATG TGTTGTGCCC TCCTGCCTCT 
GAAGGTCCGG CTGATGAGGG GGCTTTACTC AGCCAGCTCT ACTCGGCCCT CAAAGATTTT 
GATGGTCTGG AGGAGATTGA CCGTGCGCTT GGCATTCCAG CGCTAGTGGG ACAGGCTCAG 
CCTGTGGAGC AGGATCAGTT CCCTGGTCAG GACCCTAGTA TGATGATGGA TCAGAAGCCA 
CCGATGTACG GGCAGCAACA GTATGCCTCG TCCCCGGCAA ACATGCAGCA GGCTGGATAC 
ACGCCAATGC AAGATGCCAC TTTCCACGGC TTGCAAGGAC AGATGGGACA GAGACCCGGA 
TACCCAATGC TCCGAATGCA AGCGAGACCG GGACTCAGAC CCGGTGGAGT CGTCCCAAAT 
CAACCCAATA CACTCAGACT GCAGCTTCAG CACAGGCTAC AAGCACAGCA GAATCGTCAG 
CCTATGATGA ATCAGATGGG TGGAGTGTCA AATATGAACC TACCTCTCAG ACCCAACGTT 
CCAAATCAGG GGACAATCAA CGCTCAGATG CTGGCCCAGC GGCAGAGAGA GTTGCTGAGT 
AATCATTTAC GTCAGCGGCA GCTCGACCAG CAGCGGCAGC AACAGCAACA GCAGCAGCAG 
CAGCGCAGTC TGGCCATGCG GGCGCAGGGC CTCACCATGC CACCCAACAT GGCGGCAGCC 
GCAGCGGGCA TGCCAGGAGC CATGAGCAAC CCGCGGATCC CCCAGGCCAA CCCTCAGCAG 
TTCCCCTACC CTCCCAACTA CGGTACAGGA CTGGCCTCGC CGCCCCCTTC CACCAGCCCT 
TTCTCCCCCG GTTCACCCAA CCTCCCTGCC CCTCAGCTGC TCTCCCACAG CGCCATGCAT 
GGCTCTCAGA TGAGTCTAGC TAACCAGGGG ATGCTGGGCA GTCTGAGCGG ACAGTTTGGG 
GCTGTTATGA GCCCTCAAAT GCAGCACAGT GCCTTTCAGT TCCCCAGCTC AGGTATGAGT 
CAGCAGTCAG ACGGTGGGTT TGGTGGTGCC GCGACCCCTC AGAGCCCCCT CATGTCACCC 
AGGATGGGTC ACGCTCAGAG TCCCATGATG CAACAGGCGC AGGGAAACAG CTCTTTCCAG 
TCGTCTCCTG ATATGAACGG CTGGCCGCAG GGCAACATCA ACACCAACAG TATGTTTACT 
CAGCAGTCGC CACCTCAGTT CAGCCAACAA GCCAACAACA ACATGTACAA CGGCAACGGC 
ATGAACCTCA ACGTGTCCAT GGCCGCCAAC TCCAGCAACA TGGGCCAAAT GGGCAACCAG 
ATGAGCATGA GCTCCATGAA CTCTGGGCCT GGGTCCGGCA TGGCCAACAT GGGCCCTGAA 
CAGCAGAAGT ATTGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009295611.1
CDS	460..4977
Translation	MSAVGENSSDPARSEAQKRKEGPSDLLGPSPKRSTEKRNREHENKYIEELAELIFANFNDIDNFNVKPDKCAILKETVKQIRQIKEQEKAAAANEDEVQKADVSSTGQSVIDKDALGPMMLEALDGFFFVVNMEGNIVFVSENVTQYLRYNQEELMNTSVYSILHVGDHAEFIKNLLPKSHVNGVPWSSENPRRNSHTFNCRMLVNPHSEAEETQDHEAQQKYETMQCFAVSEPKSIKEEGEDFQSCLICVARRVPMKERPMLPTQESFTTRQDLQGKITSLDTSLLRASMKPGWEDLVRRCIQRFHLQNDGDISFAKRHQQEVIRHGQAFSPIYRFSLSDGTIVSAHTKSKLVRSSSTNEPQLYMSLHILQREQPVCGMASDLGNAQTMGKPMNPMSSPNTAGSSCTPQGQDATISSNTSTFPSPGAQKEPGAMHRFGCPGTMSHSATMQAATPQGSGYPLKLSSPSQGSPGMLSPRHRASPGVAGSPRLPPPQFSPAGSLHSPASMCTSSTGGNGGAGANHGYTSSSLNALQALSECHGVSHGQSLGSPDRKLGSPAAHSVAVSHHLINKMSVPESFGEQNQSEQGTPGQDEGIDLPRDEKGNLGQFGNLDGSDPQSRLRDNKSHTKLLQLLTTKTEPIESTSPPPMAGGEPGCKDGGAGNGGMGSQGGNGSHATSLKEKHKILHRLLQNSSSPVDLAKLTAEATGKELCQDSAGGTAGVPELAIKQEPVSPKKKDNALLRYLLDKDDNVLKGKGIKMEPGEIKEEGGKMTGIKTEKTDGGYDRVEPSSELDDILDDLQNSQPGLFTDSRPVSLPSAVDKQSIINDILQMTGESGANMSPQQQRALQTAVSQQNFPAPRPGQPGRAPPVRSVSLDMNMPPKGAQGQYPMMRNNNPYSIMQQQGMMGNHAVMPNQPNMVNAGVMGNNGPRVGMQQDGWGAQGTVGSAASPATAAPAGQHSLPQGALHSRMVPNPATGMPMRPNSQPGPRPMLQPQMMANAQSNMDMGMAGHQFPQQQAPPNQTAPWPDSVMPIEPVPFGNQSSSMYGGSQEDVLCPPASEGPADEGALLSQLYSALKDFDGLEEIDRALGIPALVGQAQPVEQDQFPGQDPSMMMDQKPPMYGQQQYASSPANMQQAGYTPMQDATFHGLQGQMGQRPGYPMLRMQARPGLRPGGVVPNQPNTLRLQLQHRLQAQQNRQPMMNQMGGVSNMNLPLRPNVPNQGTINAQMLAQRQRELLSNHLRQRQLDQQRQQQQQQQQQRSLAMRAQGLTMPPNMAAAAAGMPGAMSNPRIPQANPQQFPYPPNYGTGLASPPPSTSPFSPGSPNLPAPQLLSHSAMHGSQMSLANQGMLGSLSGQFGAVMSPQMQHSAFQFPSSGMSQQSDGGFGGAATPQSPLMSPRMGHAQSPMMQQAQGNSSFQSSPDMNGWPQGNINTNSMFTQQSPPQFSQQANNNMYNGNGMNLNVSMAANSSNMGQMGNQMSMSSMNSGPGSGMANMGPEQQKYC

Target ORF information:

RefSeq Version	XM_009297336.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio nuclear receptor coactivator 2 (ncoa2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009297336.3

ORF Insert Sequence:

ATGAGTGCTG TCGGGGAAAA CTCGTCTGAC CCTGCCAGGT CAGAGGCTCA GAAGCGCAAG 
GAAGGCCCAT CAGACCTCCT GGGTCCAAGT CCCAAACGAA GTACAGAAAA ACGTAACCGG 
GAACATGAAA ATAAGTACAT TGAGGAGCTC GCCGAGCTGA TTTTCGCAAA CTTCAATGAC 
ATTGACAACT TCAACGTCAA GCCTGACAAA TGTGCAATCC TAAAGGAAAC CGTGAAACAG 
ATTCGGCAAA TTAAAGAGCA AGAGAAAGCT GCTGCAGCCA ATGAGGATGA AGTGCAGAAG 
GCTGATGTGT CGTCGACAGG CCAAAGTGTT ATTGACAAAG ATGCCCTTGG ACCAATGATG 
CTGGAGGCCT TGGATGGGTT CTTCTTTGTG GTGAACATGG AGGGCAACAT TGTGTTTGTA 
TCGGAGAATG TGACGCAGTA CCTGCGCTAT AATCAGGAAG AGCTGATGAA CACCAGTGTG 
TACAGCATAC TGCATGTAGG AGACCATGCC GAATTCATCA AAAACCTCCT GCCTAAGTCA 
CACGTGAATG GAGTGCCGTG GTCCAGCGAA AACCCTCGGC GGAACAGCCA CACCTTCAAC 
TGCCGCATGC TTGTGAACCC TCACAGCGAG GCCGAAGAGA CGCAGGATCA CGAGGCACAG 
CAGAAATATG AGACTATGCA GTGTTTTGCT GTGTCTGAAC CAAAGTCCAT CAAGGAGGAG 
GGAGAGGATT TCCAGTCGTG TCTGATCTGC GTGGCGCGGC GAGTGCCCAT GAAAGAGAGA 
CCGATGCTCC CCACGCAGGA GAGCTTCACC ACCCGCCAGG ACCTCCAAGG TAAAATCACG 
TCTCTGGACA CAAGTCTGTT ACGAGCGTCT ATGAAGCCAG GCTGGGAAGA CTTGGTGCGG 
AGATGCATTC AGCGTTTTCA TCTTCAGAAC GATGGAGACA TTTCCTTCGC CAAACGACAT 
CAGCAAGAAG TGATAAGGCA CGGGCAGGCG TTCAGTCCCA TCTACCGGTT CTCCCTGTCA 
GACGGGACCA TCGTCTCTGC TCACACCAAG AGCAAACTGG TTCGCTCATC CTCTACCAAC 
GAGCCACAGC TCTACATGTC CCTGCACATC TTACAGAGGG AGCAGCCCGT CTGTGGCATG 
GCCTCTGATC TTGGAAATGC TCAGACCATG GGAAAACCCA TGAACCCCAT GAGCTCTCCA 
AACACAGCGG GTTCCTCGTG CACTCCCCAA GGGCAGGACG CCACCATCAG CAGCAACACA 
TCCACTTTCC CCTCCCCTGG TGCCCAGAAG GAGCCTGGTG CCATGCACCG TTTCGGCTGC 
CCGGGAACCA TGAGCCATTC GGCAACCATG CAAGCGGCCA CCCCTCAGGG CAGCGGGTAT 
CCACTGAAGC TGAGCAGCCC GTCTCAGGGT AGTCCGGGCA TGCTTTCTCC ACGTCACCGA 
GCCAGTCCTG GAGTAGCAGG ATCTCCACGC TTACCCCCGC CACAGTTCTC TCCAGCGGGA 
AGCCTCCATT CACCTGCCAG CATGTGCACT AGCAGTACTG GTGGCAATGG GGGAGCAGGG 
GCCAACCATG GTTACACAAG CAGTTCACTT AATGCTCTTC AGGCGCTTAG CGAATGCCAT 
GGCGTAAGCC ACGGACAATC CCTGGGATCG CCTGATAGAA AGTTAGGGTC CCCAGCTGCA 
CATTCGGTAG CAGTAAGTCA TCACCTAATT AACAAAATGA GCGTACCTGA GTCATTCGGA 
GAGCAGAACC AATCAGAGCA GGGCACCCCT GGACAGGATG AAGGCATTGA TCTGCCAAGA 
GATGAAAAGG GCAACCTCGG GCAGTTCGGC AACCTGGACG GCAGTGATCC TCAAAGCCGA 
CTGCGCGACA ACAAAAGTCA CACTAAACTC CTGCAGCTCC TCACCACTAA AACTGAGCCT 
ATCGAGTCCA CTTCACCCCC TCCAATGGCC GGTGGAGAAC CTGGGTGTAA AGACGGCGGT 
GCAGGGAATG GTGGCATGGG TTCTCAAGGC GGAAACGGGT CACATGCTAC CTCCCTGAAG 
GAGAAACACA AAATCCTTCA CAGACTCCTC CAGAACAGCT CTTCGCCTGT TGATTTGGCC 
AAACTTACAG CTGAGGCCAC AGGGAAAGAG CTGTGTCAGG ATTCTGCTGG CGGGACGGCC 
GGTGTGCCCG AGTTGGCCAT TAAACAAGAG CCTGTCAGTC CCAAGAAAAA GGACAATGCC 
TTGCTGCGCT ACCTACTGGA TAAAGATGAC AATGTACTCA AGGGTAAAGG TATTAAAATG 
GAGCCTGGAG AGATCAAGGA GGAGGGGGGC AAGATGACTG GGATTAAGAC CGAGAAAACT 
GATGGTGGCT ACGACCGTGT GGAGCCGTCA AGTGAGCTGG ATGATATCCT GGATGATCTT 
CAGAACAGTC AGCCGGGCCT GTTCACTGAC TCCAGGCCTG TGTCTCTGCC TTCTGCTGTG 
GACAAGCAGT CAATCATCAA TGACATCCTG CAGATGACAG GAGAGAGCGG AGCTAACATG 
AGTCCTCAAC AGCAGAGAGC ACTGCAGACT GCAGTCAGCC AACAGAATTT CCCTGCTCCA 
CGGCCCGGTC AGCCTGGCAG AGCTCCTCCG GTCAGAAGCG TGTCTCTGGA CATGAACATG 
CCTCCTAAAG GCGCTCAGGG TCAGTATCCT ATGATGAGGA ACAACAACCC CTACTCAATC 
ATGCAGCAGC AAGGCATGAT GGGAAACCAC GCCGTCATGC CCAATCAGCC CAACATGGTG 
AACGCAGGAG TCATGGGTAA TAATGGTCCC CGTGTAGGCA TGCAGCAGGA CGGTTGGGGT 
GCTCAGGGTA CCGTCGGCAG CGCAGCTTCA CCTGCCACAG CTGCTCCCGC TGGCCAGCAC 
TCTCTCCCGC AGGGGGCACT GCACAGCCGC ATGGTGCCCA ACCCTGCCAC AGGAATGCCC 
ATGAGACCCA ACAGCCAGCC TGGACCCAGA CCAATGCTGC AACCGCAGAT GATGGCCAAT 
GCTCAGTCAA ACATGGACAT GGGCATGGCT GGCCACCAGT TTCCCCAGCA ACAGGCACCG 
CCCAATCAGA CGGCTCCATG GCCAGACAGT GTGATGCCTA TAGAGCCGGT GCCGTTTGGG 
AACCAGAGCA GTTCCATGTA CGGCGGGTCT CAGGAGGATG TGTTGTGCCC TCCTGCCTCT 
GAAGGTCCGG CTGATGAGGG GGCTTTACTC AGCCAGCTCT ACTCGGCCCT CAAAGATTTT 
GATGGTCTGG AGGAGATTGA CCGTGCGCTT GGCATTCCAG CGCTAGTGGG ACAGGCTCAG 
CCTGTGGAGC AGGATCAGTT CCCTGGTCAG GACCCTAGTA TGATGATGGA TCAGAAGCCA 
CCGATGTACG GGCAGCAACA GTATGCCTCG TCCCCGGCAA ACATGCAGCA GGCTGGATAC 
ACGCCAATGC AAGATGCCAC TTTCCACGGC TTGCAAGGAC AGATGGGACA GAGACCCGGA 
TACCCAATGC TCCGAATGCA AGCGAGACCG GGACTCAGAC CCGGTGGAGT CGTCCCAAAT 
CAACCCAATA CACTCAGACT GCAGCTTCAG CACAGGCTAC AAGCACAGCA GAATCGTCAG 
CCTATGATGA ATCAGATGGG TGGAGTGTCA AATATGAACC TACCTCTCAG ACCCAACGTT 
CCAAATCAGG GGACAATCAA CGCTCAGATG CTGGCCCAGC GGCAGAGAGA GTTGCTGAGT 
AATCATTTAC GTCAGCGGCA GCTCGACCAG CAGCGGCAGC AACAGCAACA GCAGCAGCAG 
CAGCGCAGTC TGGCCATGCG GGCGCAGGGC CTCACCATGC CACCCAACAT GGCGGCAGCC 
GCAGCGGGCA TGCCAGGAGC CATGAGCAAC CCGCGGATCC CCCAGGCCAA CCCTCAGCAG 
TTCCCCTACC CTCCCAACTA CGGTACAGGA CTGGCCTCGC CGCCCCCTTC CACCAGCCCT 
TTCTCCCCCG GTTCACCCAA CCTCCCTGCC CCTCAGCTGC TCTCCCACAG CGCCATGCAT 
GGCTCTCAGA TGAGTCTAGC TAACCAGGGG ATGCTGGGCA GTCTGAGCGG ACAGTTTGGG 
GCTGTTATGA GCCCTCAAAT GCAGCACAGT GCCTTTCAGT TCCCCAGCTC AGGTATGAGT 
CAGCAGTCAG ACGGTGGGTT TGGTGGTGCC GCGACCCCTC AGAGCCCCCT CATGTCACCC 
AGGATGGGTC ACGCTCAGAG TCCCATGATG CAACAGGCGC AGGGAAACAG CTCTTTCCAG 
TCGTCTCCTG ATATGAACGG CTGGCCGCAG GGCAACATCA ACACCAACAG TATGTTTACT 
CAGCAGTCGC CACCTCAGTT CAGCCAACAA GCCAACAACA ACATGTACAA CGGCAACGGC 
ATGAACCTCA ACGTGTCCAT GGCCGCCAAC TCCAGCAACA TGGGCCAAAT GGGCAACCAG 
ATGAGCATGA GCTCCATGAA CTCTGGGCCT GGGTCCGGCA TGGCCAACAT GGGCCCTGAA 
CAGCAGAAGT ATTGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ncoa2 ( XM_009297337.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009295612.1 Danio rerio nuclear receptor coactivator 2 (ncoa2), transcript variant X2, mRNA.

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CloneID	ODa39441
Clone ID Related Accession (Same CDS sequence)	XM_009297337.2 , XM_021470228.1
Accession Version	XM_009297337.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4515bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	nuclear receptor coactivator 2 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007135.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009297337.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGCTG TCGGGGAAAA CTCGTCTGAC CCTGCCAGGT CAGAGGCTCA GAAGCGCAAG 
GAAGGCCCAT CAGACCTCCT GGGTCCAAGT CCCAAACGAA GTACAGAAAA ACGTAACCGG 
GAACATGAAA ATAAGTACAT TGAGGAGCTC GCCGAGCTGA TTTTCGCAAA CTTCAATGAC 
ATTGACAACT TCAACGTCAA GCCTGACAAA TGTGCAATCC TAAAGGAAAC CGTGAAACAG 
ATTCGGCAAA TTAAAGAGCA AGAGAAAGCT GCTGCAGCCA ATGAGGATGA AGTGCAGAAG 
GCTGATGTGT CGTCGACAGG CCAAAGTGTT ATTGACAAAG ATGCCCTTGG ACCAATGATG 
CTGGAGGCCT TGGATGGGTT CTTCTTTGTG GTGAACATGG AGGGCAACAT TGTGTTTGTA 
TCGGAGAATG TGACGCAGTA CCTGCGCTAT AATCAGGAAG AGCTGATGAA CACCAGTGTG 
TACAGCATAC TGCATGTAGG AGACCATGCC GAATTCATCA AAAACCTCCT GCCTAAGTCA 
CACGTGAATG GAGTGCCGTG GTCCAGCGAA AACCCTCGGC GGAACAGCCA CACCTTCAAC 
TGCCGCATGC TTGTGAACCC TCACAGCGAG GCCGAAGAGA CGCAGGATCA CGAGGCACAG 
CAGAAATATG AGACTATGCA GTGTTTTGCT GTGTCTGAAC CAAAGTCCAT CAAGGAGGAG 
GGAGAGGATT TCCAGTCGTG TCTGATCTGC GTGGCGCGGC GAGTGCCCAT GAAAGAGAGA 
CCGATGCTCC CCACGCAGGA GAGCTTCACC ACCCGCCAGG ACCTCCAAGG TAAAATCACG 
TCTCTGGACA CAAGTCTGTT ACGAGCGTCT ATGAAGCCAG GCTGGGAAGA CTTGGTGCGG 
AGATGCATTC AGCGTTTTCA TCTTCAGAAC GATGGAGACA TTTCCTTCGC CAAACGACAT 
CAGCAAGAAG TGATAAGGCA CGGGCAGGCG TTCAGTCCCA TCTACCGGTT CTCCCTGTCA 
GACGGGACCA TCGTCTCTGC TCACACCAAG AGCAAACTGG TTCGCTCATC CTCTACCAAC 
GAGCCACAGC TCTACATGTC CCTGCACATC TTACAGAGGG AGCAGCCCGT CTGTGGCATG 
GCCTCTGATC TTGGAAATGC TCAGACCATG GGAAAACCCA TGAACCCCAT GAGCTCTCCA 
AACACAGCGG GTTCCTCGTG CACTCCCCAA GGGCAGGACG CCACCATCAG CAGCAACACA 
TCCACTTTCC CCTCCCCTGG TGCCCAGAAG GAGCCTGGTG CCATGCACCG TTTCGGCTGC 
CCGGGAACCA TGAGCCATTC GGCAACCATG CAAGCGGCCA CCCCTCAGGG CAGCGGGTAT 
CCACTGAAGC TGAGCAGCCC GTCTCAGGGT AGTCCGGGCA TGCTTTCTCC ACGTCACCGA 
GCCAGTCCTG GAGTAGCAGG ATCTCCACGC TTACCCCCGC CACAGTTCTC TCCAGCGGGA 
AGCCTCCATT CACCTGCCAG CATGTGCACT AGCAGTACTG GTGGCAATGG GGGAGCAGGG 
GCCAACCATG GTTACACAAG CAGTTCACTT AATGCTCTTC AGGCGCTTAG CGAATGCCAT 
GGCGTAAGCC ACGGACAATC CCTGGGATCG CCTGATAGAA AGTTAGGGTC CCCAGCTGCA 
CATTCGGTAG CAGTAAGTCA TCACCTAATT AACAAAATGA GCGTACCTGA GTCATTCGGA 
GAGCAGAACC AATCAGAGCA GGGCACCCCT GGACAGGATG AAGGCATTGA TCTGCCAAGA 
GATGAAAAGG GCAACCTCGG GCAGTTCGGC AACCTGGACG GCAGTGATCC TCAAAGCCGA 
CTGCGCGACA ACAAAAGTCA CACTAAACTC CTGCAGCTCC TCACCACTAA AACTGAGCCT 
ATCGAGTCCA CTTCACCCCC TCCAATGGCC GGTGGAGAAC CTGGGTGTAA AGACGGCGGT 
GCAGGGAATG GTGGCATGGG TTCTCAAGGC GGAAACGGGT CACATGCTAC CTCCCTGAAG 
GAGAAACACA AAATCCTTCA CAGACTCCTC CAGAACAGCT CTTCGCCTGT TGATTTGGCC 
AAACTTACAG CTGAGGCCAC AGGGAAAGAG CTGTGTCAGG ATTCTGCTGG CGGGACGGCC 
GGTGTGCCCG AGTTGGCCAT TAAACAAGAG CCTGTCAGTC CCAAGAAAAA GGACAATGCC 
TTGCTGCGCT ACCTACTGGA TAAAGATGAC AATGTACTCA AGGGTAAAGG TATTAAAATG 
GAGCCTGGAG AGATCAAGGA GGAGGGGGGC AAGATGACTG GGATTAAGAC CGAGAAAACT 
GATGGTGGCT ACGACCGTGT GGAGCCGTCA AGTGAGCTGG ATGATATCCT GGATGATCTT 
CAGAACAGTC AGCCGGGCCT GTTCACTGAC TCCAGGCCTG TGTCTCTGCC TTCTGCTGTG 
GACAAGCAGT CAATCATCAA TGACATCCTG CAGATGACAG GAGAGAGCGG AGCTAACATG 
AGTCCTCAAC AGCAGAGAGC ACTGCAGACT GCAGTCAGCC AACAGAATTT CCCTGCTCCA 
CGGCCCGGTC AGCCTGGCAG AGCTCCTCCG GTCAGAAGCG TGTCTCTGGA CATGAACATG 
CCTCCTAAAG GCGCTCAGGG TCAGTATCCT ATGATGAGGA ACAACAACCC CTACTCAATC 
ATGCAGCAGC AAGGCATGAT GGGAAACCAC GCCGTCATGC CCAATCAGCC CAACATGGTG 
AACGCAGGAG TCATGGGTAA TAATGGTCCC CGTGTAGGCA TGCAGCAGGA CGGTTGGGGT 
GCTCAGGGTA CCGTCGGCAG CGCAGCTTCA CCTGCCACAG CTGCTCCCGC TGGCCAGCAC 
TCTCTCCCGC AGGGGGCACT GCACAGCCGC ATGGTGCCCA ACCCTGCCAC AGGAATGCCC 
ATGAGACCCA ACAGCCAGCC TGGACCCAGA CCAATGCTGC AACCGCAGAT GATGGCCAAT 
GCTCAGTCAA ACATGGACAT GGGCATGGCT GGCCACCAGT TTCCCCAGCA ACAGGCACCG 
CCCAATCAGA CGGCTCCATG GCCAGACAGT GTGATGCCTA TAGAGCCGGT GCCGTTTGGG 
AACCAGAGCA GTTCCATGTA CGGCGGGTCT CAGGAGGATG TGTTGTGCCC TCCTGCCTCT 
GAAGGTCCGG CTGATGAGGG GGCTTTACTC AGCCAGCTCT ACTCGGCCCT CAAAGATTTT 
GATGGTCTGG AGGAGATTGA CCGTGCGCTT GGCATTCCAG CGCTAGTGGG ACAGGCTCAG 
CCTGTGGAGC AGGATCAGTT CCCTGGTCAG GACCCTAGTA TGATGATGGA TCAGAAGCCA 
CCGATGTACG GGCAGCAACA GTATGCCTCG TCCCCGGCAA ACATGCAGCA GGCTGGATAC 
ACGCCAATGC AAGATGCCAC TTTCCACGGC TTGCAAGGAC AGATGGGACA GAGACCCGGA 
TACCCAATGC TCCGAATGCA AGCGAGACCG GGACTCAGAC CCGGTGGAGT CGTCCCAAAT 
CAACCCAATA CACTCAGACT GCAGCTTCAG CACAGGCTAC AAGCACAGCA GAATCGTCAG 
CCTATGATGA ATCAGATGGG TGGAGTGTCA AATATGAACC TACCTCTCAG ACCCAACGTT 
CCAAATCAGG GGACAATCAA CGCTCAGATG CTGGCCCAGC GGCAGAGAGA GTTGCTGAGT 
AATCATTTAC GTCAGCGGCA GCTCGACCAG CAGCGGCAGC AACAGCAACA GCAGCAGCAG 
CAGCGCAGTC TGGCCATGCG GGCGCAGGGC CTCACCATGC CACCCAACAT GGCGGCAGCC 
GCAGCGGGCA TGCCAGGAGC CATGAGCAAC CCGCGGATCC CCCAGGCCAA CCCTCAGCAG 
TTCCCCTACC CTCCCAACTA CGGTACAGGA CTGGCCTCGC CGCCCCCTTC CACCAGCCCT 
TTCTCCCCCG GTTCACCCAA CCTCCCTGCC CCTCAGCTGC TCTCCCACAG CGCCATGCAT 
GGCTCTCAGA TGAGTCTAGC TAACCAGGGG ATGCTGGGCA GTCTGAGCGG ACAGTTTGGG 
GCTGTTATGA GCCCTCAAAT GCAGCACAGT GCCTTTCAGT TCCCCAGCTC AGGTATGAGT 
CAGCAGTCAG ACGGTGGGTT TGGTGGTGCC GCGACCCCTC AGAGCCCCCT CATGTCACCC 
AGGATGGGTC ACGCTCAGAG TCCCATGATG CAACAGGCGC AGGGAAACAG CTCTTTCCAG 
TCGTCTCCTG ATATGAACGG CTGGCCGCAG GGCAACATCA ACACCAACAG TATGTTTACT 
CAGCAGTCGC CACCTCAGTT CAGCCAACAA GCCAACAACA ACATGTACAA CGGCAACGGC 
ATGAACCTCA ACGTGTCCAT GGCCGCCAAC TCCAGCAACA TGGGCCAAAT GGGCAACCAG 
ATGAGCATGA GCTCCATGAA CTCTGGGCCT GGGTCCGGCA TGGCCAACAT GGGCCCTGAA 
CAGAAGTATT GTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009295612.1
CDS	1294..5808
Translation	MSAVGENSSDPARSEAQKRKEGPSDLLGPSPKRSTEKRNREHENKYIEELAELIFANFNDIDNFNVKPDKCAILKETVKQIRQIKEQEKAAAANEDEVQKADVSSTGQSVIDKDALGPMMLEALDGFFFVVNMEGNIVFVSENVTQYLRYNQEELMNTSVYSILHVGDHAEFIKNLLPKSHVNGVPWSSENPRRNSHTFNCRMLVNPHSEAEETQDHEAQQKYETMQCFAVSEPKSIKEEGEDFQSCLICVARRVPMKERPMLPTQESFTTRQDLQGKITSLDTSLLRASMKPGWEDLVRRCIQRFHLQNDGDISFAKRHQQEVIRHGQAFSPIYRFSLSDGTIVSAHTKSKLVRSSSTNEPQLYMSLHILQREQPVCGMASDLGNAQTMGKPMNPMSSPNTAGSSCTPQGQDATISSNTSTFPSPGAQKEPGAMHRFGCPGTMSHSATMQAATPQGSGYPLKLSSPSQGSPGMLSPRHRASPGVAGSPRLPPPQFSPAGSLHSPASMCTSSTGGNGGAGANHGYTSSSLNALQALSECHGVSHGQSLGSPDRKLGSPAAHSVAVSHHLINKMSVPESFGEQNQSEQGTPGQDEGIDLPRDEKGNLGQFGNLDGSDPQSRLRDNKSHTKLLQLLTTKTEPIESTSPPPMAGGEPGCKDGGAGNGGMGSQGGNGSHATSLKEKHKILHRLLQNSSSPVDLAKLTAEATGKELCQDSAGGTAGVPELAIKQEPVSPKKKDNALLRYLLDKDDNVLKGKGIKMEPGEIKEEGGKMTGIKTEKTDGGYDRVEPSSELDDILDDLQNSQPGLFTDSRPVSLPSAVDKQSIINDILQMTGESGANMSPQQQRALQTAVSQQNFPAPRPGQPGRAPPVRSVSLDMNMPPKGAQGQYPMMRNNNPYSIMQQQGMMGNHAVMPNQPNMVNAGVMGNNGPRVGMQQDGWGAQGTVGSAASPATAAPAGQHSLPQGALHSRMVPNPATGMPMRPNSQPGPRPMLQPQMMANAQSNMDMGMAGHQFPQQQAPPNQTAPWPDSVMPIEPVPFGNQSSSMYGGSQEDVLCPPASEGPADEGALLSQLYSALKDFDGLEEIDRALGIPALVGQAQPVEQDQFPGQDPSMMMDQKPPMYGQQQYASSPANMQQAGYTPMQDATFHGLQGQMGQRPGYPMLRMQARPGLRPGGVVPNQPNTLRLQLQHRLQAQQNRQPMMNQMGGVSNMNLPLRPNVPNQGTINAQMLAQRQRELLSNHLRQRQLDQQRQQQQQQQQQRSLAMRAQGLTMPPNMAAAAAGMPGAMSNPRIPQANPQQFPYPPNYGTGLASPPPSTSPFSPGSPNLPAPQLLSHSAMHGSQMSLANQGMLGSLSGQFGAVMSPQMQHSAFQFPSSGMSQQSDGGFGGAATPQSPLMSPRMGHAQSPMMQQAQGNSSFQSSPDMNGWPQGNINTNSMFTQQSPPQFSQQANNNMYNGNGMNLNVSMAANSSNMGQMGNQMSMSSMNSGPGSGMANMGPEQKYC

Target ORF information:

RefSeq Version	XM_009297337.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio nuclear receptor coactivator 2 (ncoa2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009297337.2

ORF Insert Sequence:

ATGAGTGCTG TCGGGGAAAA CTCGTCTGAC CCTGCCAGGT CAGAGGCTCA GAAGCGCAAG 
GAAGGCCCAT CAGACCTCCT GGGTCCAAGT CCCAAACGAA GTACAGAAAA ACGTAACCGG 
GAACATGAAA ATAAGTACAT TGAGGAGCTC GCCGAGCTGA TTTTCGCAAA CTTCAATGAC 
ATTGACAACT TCAACGTCAA GCCTGACAAA TGTGCAATCC TAAAGGAAAC CGTGAAACAG 
ATTCGGCAAA TTAAAGAGCA AGAGAAAGCT GCTGCAGCCA ATGAGGATGA AGTGCAGAAG 
GCTGATGTGT CGTCGACAGG CCAAAGTGTT ATTGACAAAG ATGCCCTTGG ACCAATGATG 
CTGGAGGCCT TGGATGGGTT CTTCTTTGTG GTGAACATGG AGGGCAACAT TGTGTTTGTA 
TCGGAGAATG TGACGCAGTA CCTGCGCTAT AATCAGGAAG AGCTGATGAA CACCAGTGTG 
TACAGCATAC TGCATGTAGG AGACCATGCC GAATTCATCA AAAACCTCCT GCCTAAGTCA 
CACGTGAATG GAGTGCCGTG GTCCAGCGAA AACCCTCGGC GGAACAGCCA CACCTTCAAC 
TGCCGCATGC TTGTGAACCC TCACAGCGAG GCCGAAGAGA CGCAGGATCA CGAGGCACAG 
CAGAAATATG AGACTATGCA GTGTTTTGCT GTGTCTGAAC CAAAGTCCAT CAAGGAGGAG 
GGAGAGGATT TCCAGTCGTG TCTGATCTGC GTGGCGCGGC GAGTGCCCAT GAAAGAGAGA 
CCGATGCTCC CCACGCAGGA GAGCTTCACC ACCCGCCAGG ACCTCCAAGG TAAAATCACG 
TCTCTGGACA CAAGTCTGTT ACGAGCGTCT ATGAAGCCAG GCTGGGAAGA CTTGGTGCGG 
AGATGCATTC AGCGTTTTCA TCTTCAGAAC GATGGAGACA TTTCCTTCGC CAAACGACAT 
CAGCAAGAAG TGATAAGGCA CGGGCAGGCG TTCAGTCCCA TCTACCGGTT CTCCCTGTCA 
GACGGGACCA TCGTCTCTGC TCACACCAAG AGCAAACTGG TTCGCTCATC CTCTACCAAC 
GAGCCACAGC TCTACATGTC CCTGCACATC TTACAGAGGG AGCAGCCCGT CTGTGGCATG 
GCCTCTGATC TTGGAAATGC TCAGACCATG GGAAAACCCA TGAACCCCAT GAGCTCTCCA 
AACACAGCGG GTTCCTCGTG CACTCCCCAA GGGCAGGACG CCACCATCAG CAGCAACACA 
TCCACTTTCC CCTCCCCTGG TGCCCAGAAG GAGCCTGGTG CCATGCACCG TTTCGGCTGC 
CCGGGAACCA TGAGCCATTC GGCAACCATG CAAGCGGCCA CCCCTCAGGG CAGCGGGTAT 
CCACTGAAGC TGAGCAGCCC GTCTCAGGGT AGTCCGGGCA TGCTTTCTCC ACGTCACCGA 
GCCAGTCCTG GAGTAGCAGG ATCTCCACGC TTACCCCCGC CACAGTTCTC TCCAGCGGGA 
AGCCTCCATT CACCTGCCAG CATGTGCACT AGCAGTACTG GTGGCAATGG GGGAGCAGGG 
GCCAACCATG GTTACACAAG CAGTTCACTT AATGCTCTTC AGGCGCTTAG CGAATGCCAT 
GGCGTAAGCC ACGGACAATC CCTGGGATCG CCTGATAGAA AGTTAGGGTC CCCAGCTGCA 
CATTCGGTAG CAGTAAGTCA TCACCTAATT AACAAAATGA GCGTACCTGA GTCATTCGGA 
GAGCAGAACC AATCAGAGCA GGGCACCCCT GGACAGGATG AAGGCATTGA TCTGCCAAGA 
GATGAAAAGG GCAACCTCGG GCAGTTCGGC AACCTGGACG GCAGTGATCC TCAAAGCCGA 
CTGCGCGACA ACAAAAGTCA CACTAAACTC CTGCAGCTCC TCACCACTAA AACTGAGCCT 
ATCGAGTCCA CTTCACCCCC TCCAATGGCC GGTGGAGAAC CTGGGTGTAA AGACGGCGGT 
GCAGGGAATG GTGGCATGGG TTCTCAAGGC GGAAACGGGT CACATGCTAC CTCCCTGAAG 
GAGAAACACA AAATCCTTCA CAGACTCCTC CAGAACAGCT CTTCGCCTGT TGATTTGGCC 
AAACTTACAG CTGAGGCCAC AGGGAAAGAG CTGTGTCAGG ATTCTGCTGG CGGGACGGCC 
GGTGTGCCCG AGTTGGCCAT TAAACAAGAG CCTGTCAGTC CCAAGAAAAA GGACAATGCC 
TTGCTGCGCT ACCTACTGGA TAAAGATGAC AATGTACTCA AGGGTAAAGG TATTAAAATG 
GAGCCTGGAG AGATCAAGGA GGAGGGGGGC AAGATGACTG GGATTAAGAC CGAGAAAACT 
GATGGTGGCT ACGACCGTGT GGAGCCGTCA AGTGAGCTGG ATGATATCCT GGATGATCTT 
CAGAACAGTC AGCCGGGCCT GTTCACTGAC TCCAGGCCTG TGTCTCTGCC TTCTGCTGTG 
GACAAGCAGT CAATCATCAA TGACATCCTG CAGATGACAG GAGAGAGCGG AGCTAACATG 
AGTCCTCAAC AGCAGAGAGC ACTGCAGACT GCAGTCAGCC AACAGAATTT CCCTGCTCCA 
CGGCCCGGTC AGCCTGGCAG AGCTCCTCCG GTCAGAAGCG TGTCTCTGGA CATGAACATG 
CCTCCTAAAG GCGCTCAGGG TCAGTATCCT ATGATGAGGA ACAACAACCC CTACTCAATC 
ATGCAGCAGC AAGGCATGAT GGGAAACCAC GCCGTCATGC CCAATCAGCC CAACATGGTG 
AACGCAGGAG TCATGGGTAA TAATGGTCCC CGTGTAGGCA TGCAGCAGGA CGGTTGGGGT 
GCTCAGGGTA CCGTCGGCAG CGCAGCTTCA CCTGCCACAG CTGCTCCCGC TGGCCAGCAC 
TCTCTCCCGC AGGGGGCACT GCACAGCCGC ATGGTGCCCA ACCCTGCCAC AGGAATGCCC 
ATGAGACCCA ACAGCCAGCC TGGACCCAGA CCAATGCTGC AACCGCAGAT GATGGCCAAT 
GCTCAGTCAA ACATGGACAT GGGCATGGCT GGCCACCAGT TTCCCCAGCA ACAGGCACCG 
CCCAATCAGA CGGCTCCATG GCCAGACAGT GTGATGCCTA TAGAGCCGGT GCCGTTTGGG 
AACCAGAGCA GTTCCATGTA CGGCGGGTCT CAGGAGGATG TGTTGTGCCC TCCTGCCTCT 
GAAGGTCCGG CTGATGAGGG GGCTTTACTC AGCCAGCTCT ACTCGGCCCT CAAAGATTTT 
GATGGTCTGG AGGAGATTGA CCGTGCGCTT GGCATTCCAG CGCTAGTGGG ACAGGCTCAG 
CCTGTGGAGC AGGATCAGTT CCCTGGTCAG GACCCTAGTA TGATGATGGA TCAGAAGCCA 
CCGATGTACG GGCAGCAACA GTATGCCTCG TCCCCGGCAA ACATGCAGCA GGCTGGATAC 
ACGCCAATGC AAGATGCCAC TTTCCACGGC TTGCAAGGAC AGATGGGACA GAGACCCGGA 
TACCCAATGC TCCGAATGCA AGCGAGACCG GGACTCAGAC CCGGTGGAGT CGTCCCAAAT 
CAACCCAATA CACTCAGACT GCAGCTTCAG CACAGGCTAC AAGCACAGCA GAATCGTCAG 
CCTATGATGA ATCAGATGGG TGGAGTGTCA AATATGAACC TACCTCTCAG ACCCAACGTT 
CCAAATCAGG GGACAATCAA CGCTCAGATG CTGGCCCAGC GGCAGAGAGA GTTGCTGAGT 
AATCATTTAC GTCAGCGGCA GCTCGACCAG CAGCGGCAGC AACAGCAACA GCAGCAGCAG 
CAGCGCAGTC TGGCCATGCG GGCGCAGGGC CTCACCATGC CACCCAACAT GGCGGCAGCC 
GCAGCGGGCA TGCCAGGAGC CATGAGCAAC CCGCGGATCC CCCAGGCCAA CCCTCAGCAG 
TTCCCCTACC CTCCCAACTA CGGTACAGGA CTGGCCTCGC CGCCCCCTTC CACCAGCCCT 
TTCTCCCCCG GTTCACCCAA CCTCCCTGCC CCTCAGCTGC TCTCCCACAG CGCCATGCAT 
GGCTCTCAGA TGAGTCTAGC TAACCAGGGG ATGCTGGGCA GTCTGAGCGG ACAGTTTGGG 
GCTGTTATGA GCCCTCAAAT GCAGCACAGT GCCTTTCAGT TCCCCAGCTC AGGTATGAGT 
CAGCAGTCAG ACGGTGGGTT TGGTGGTGCC GCGACCCCTC AGAGCCCCCT CATGTCACCC 
AGGATGGGTC ACGCTCAGAG TCCCATGATG CAACAGGCGC AGGGAAACAG CTCTTTCCAG 
TCGTCTCCTG ATATGAACGG CTGGCCGCAG GGCAACATCA ACACCAACAG TATGTTTACT 
CAGCAGTCGC CACCTCAGTT CAGCCAACAA GCCAACAACA ACATGTACAA CGGCAACGGC 
ATGAACCTCA ACGTGTCCAT GGCCGCCAAC TCCAGCAACA TGGGCCAAAT GGGCAACCAG 
ATGAGCATGA GCTCCATGAA CTCTGGGCCT GGGTCCGGCA TGGCCAACAT GGGCCCTGAA 
CAGAAGTATT GTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ncoa2 ( XM_021470228.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021325903.1 Danio rerio nuclear receptor coactivator 2 (ncoa2), transcript variant X2, mRNA.

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CloneID	ODa39441
Clone ID Related Accession (Same CDS sequence)	XM_009297337.2 , XM_021470228.1
Accession Version	XM_021470228.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4515bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	nuclear receptor coactivator 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007135.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGCTG TCGGGGAAAA CTCGTCTGAC CCTGCCAGGT CAGAGGCTCA GAAGCGCAAG 
GAAGGCCCAT CAGACCTCCT GGGTCCAAGT CCCAAACGAA GTACAGAAAA ACGTAACCGG 
GAACATGAAA ATAAGTACAT TGAGGAGCTC GCCGAGCTGA TTTTCGCAAA CTTCAATGAC 
ATTGACAACT TCAACGTCAA GCCTGACAAA TGTGCAATCC TAAAGGAAAC CGTGAAACAG 
ATTCGGCAAA TTAAAGAGCA AGAGAAAGCT GCTGCAGCCA ATGAGGATGA AGTGCAGAAG 
GCTGATGTGT CGTCGACAGG CCAAAGTGTT ATTGACAAAG ATGCCCTTGG ACCAATGATG 
CTGGAGGCCT TGGATGGGTT CTTCTTTGTG GTGAACATGG AGGGCAACAT TGTGTTTGTA 
TCGGAGAATG TGACGCAGTA CCTGCGCTAT AATCAGGAAG AGCTGATGAA CACCAGTGTG 
TACAGCATAC TGCATGTAGG AGACCATGCC GAATTCATCA AAAACCTCCT GCCTAAGTCA 
CACGTGAATG GAGTGCCGTG GTCCAGCGAA AACCCTCGGC GGAACAGCCA CACCTTCAAC 
TGCCGCATGC TTGTGAACCC TCACAGCGAG GCCGAAGAGA CGCAGGATCA CGAGGCACAG 
CAGAAATATG AGACTATGCA GTGTTTTGCT GTGTCTGAAC CAAAGTCCAT CAAGGAGGAG 
GGAGAGGATT TCCAGTCGTG TCTGATCTGC GTGGCGCGGC GAGTGCCCAT GAAAGAGAGA 
CCGATGCTCC CCACGCAGGA GAGCTTCACC ACCCGCCAGG ACCTCCAAGG TAAAATCACG 
TCTCTGGACA CAAGTCTGTT ACGAGCGTCT ATGAAGCCAG GCTGGGAAGA CTTGGTGCGG 
AGATGCATTC AGCGTTTTCA TCTTCAGAAC GATGGAGACA TTTCCTTCGC CAAACGACAT 
CAGCAAGAAG TGATAAGGCA CGGGCAGGCG TTCAGTCCCA TCTACCGGTT CTCCCTGTCA 
GACGGGACCA TCGTCTCTGC TCACACCAAG AGCAAACTGG TTCGCTCATC CTCTACCAAC 
GAGCCACAGC TCTACATGTC CCTGCACATC TTACAGAGGG AGCAGCCCGT CTGTGGCATG 
GCCTCTGATC TTGGAAATGC TCAGACCATG GGAAAACCCA TGAACCCCAT GAGCTCTCCA 
AACACAGCGG GTTCCTCGTG CACTCCCCAA GGGCAGGACG CCACCATCAG CAGCAACACA 
TCCACTTTCC CCTCCCCTGG TGCCCAGAAG GAGCCTGGTG CCATGCACCG TTTCGGCTGC 
CCGGGAACCA TGAGCCATTC GGCAACCATG CAAGCGGCCA CCCCTCAGGG CAGCGGGTAT 
CCACTGAAGC TGAGCAGCCC GTCTCAGGGT AGTCCGGGCA TGCTTTCTCC ACGTCACCGA 
GCCAGTCCTG GAGTAGCAGG ATCTCCACGC TTACCCCCGC CACAGTTCTC TCCAGCGGGA 
AGCCTCCATT CACCTGCCAG CATGTGCACT AGCAGTACTG GTGGCAATGG GGGAGCAGGG 
GCCAACCATG GTTACACAAG CAGTTCACTT AATGCTCTTC AGGCGCTTAG CGAATGCCAT 
GGCGTAAGCC ACGGACAATC CCTGGGATCG CCTGATAGAA AGTTAGGGTC CCCAGCTGCA 
CATTCGGTAG CAGTAAGTCA TCACCTAATT AACAAAATGA GCGTACCTGA GTCATTCGGA 
GAGCAGAACC AATCAGAGCA GGGCACCCCT GGACAGGATG AAGGCATTGA TCTGCCAAGA 
GATGAAAAGG GCAACCTCGG GCAGTTCGGC AACCTGGACG GCAGTGATCC TCAAAGCCGA 
CTGCGCGACA ACAAAAGTCA CACTAAACTC CTGCAGCTCC TCACCACTAA AACTGAGCCT 
ATCGAGTCCA CTTCACCCCC TCCAATGGCC GGTGGAGAAC CTGGGTGTAA AGACGGCGGT 
GCAGGGAATG GTGGCATGGG TTCTCAAGGC GGAAACGGGT CACATGCTAC CTCCCTGAAG 
GAGAAACACA AAATCCTTCA CAGACTCCTC CAGAACAGCT CTTCGCCTGT TGATTTGGCC 
AAACTTACAG CTGAGGCCAC AGGGAAAGAG CTGTGTCAGG ATTCTGCTGG CGGGACGGCC 
GGTGTGCCCG AGTTGGCCAT TAAACAAGAG CCTGTCAGTC CCAAGAAAAA GGACAATGCC 
TTGCTGCGCT ACCTACTGGA TAAAGATGAC AATGTACTCA AGGGTAAAGG TATTAAAATG 
GAGCCTGGAG AGATCAAGGA GGAGGGGGGC AAGATGACTG GGATTAAGAC CGAGAAAACT 
GATGGTGGCT ACGACCGTGT GGAGCCGTCA AGTGAGCTGG ATGATATCCT GGATGATCTT 
CAGAACAGTC AGCCGGGCCT GTTCACTGAC TCCAGGCCTG TGTCTCTGCC TTCTGCTGTG 
GACAAGCAGT CAATCATCAA TGACATCCTG CAGATGACAG GAGAGAGCGG AGCTAACATG 
AGTCCTCAAC AGCAGAGAGC ACTGCAGACT GCAGTCAGCC AACAGAATTT CCCTGCTCCA 
CGGCCCGGTC AGCCTGGCAG AGCTCCTCCG GTCAGAAGCG TGTCTCTGGA CATGAACATG 
CCTCCTAAAG GCGCTCAGGG TCAGTATCCT ATGATGAGGA ACAACAACCC CTACTCAATC 
ATGCAGCAGC AAGGCATGAT GGGAAACCAC GCCGTCATGC CCAATCAGCC CAACATGGTG 
AACGCAGGAG TCATGGGTAA TAATGGTCCC CGTGTAGGCA TGCAGCAGGA CGGTTGGGGT 
GCTCAGGGTA CCGTCGGCAG CGCAGCTTCA CCTGCCACAG CTGCTCCCGC TGGCCAGCAC 
TCTCTCCCGC AGGGGGCACT GCACAGCCGC ATGGTGCCCA ACCCTGCCAC AGGAATGCCC 
ATGAGACCCA ACAGCCAGCC TGGACCCAGA CCAATGCTGC AACCGCAGAT GATGGCCAAT 
GCTCAGTCAA ACATGGACAT GGGCATGGCT GGCCACCAGT TTCCCCAGCA ACAGGCACCG 
CCCAATCAGA CGGCTCCATG GCCAGACAGT GTGATGCCTA TAGAGCCGGT GCCGTTTGGG 
AACCAGAGCA GTTCCATGTA CGGCGGGTCT CAGGAGGATG TGTTGTGCCC TCCTGCCTCT 
GAAGGTCCGG CTGATGAGGG GGCTTTACTC AGCCAGCTCT ACTCGGCCCT CAAAGATTTT 
GATGGTCTGG AGGAGATTGA CCGTGCGCTT GGCATTCCAG CGCTAGTGGG ACAGGCTCAG 
CCTGTGGAGC AGGATCAGTT CCCTGGTCAG GACCCTAGTA TGATGATGGA TCAGAAGCCA 
CCGATGTACG GGCAGCAACA GTATGCCTCG TCCCCGGCAA ACATGCAGCA GGCTGGATAC 
ACGCCAATGC AAGATGCCAC TTTCCACGGC TTGCAAGGAC AGATGGGACA GAGACCCGGA 
TACCCAATGC TCCGAATGCA AGCGAGACCG GGACTCAGAC CCGGTGGAGT CGTCCCAAAT 
CAACCCAATA CACTCAGACT GCAGCTTCAG CACAGGCTAC AAGCACAGCA GAATCGTCAG 
CCTATGATGA ATCAGATGGG TGGAGTGTCA AATATGAACC TACCTCTCAG ACCCAACGTT 
CCAAATCAGG GGACAATCAA CGCTCAGATG CTGGCCCAGC GGCAGAGAGA GTTGCTGAGT 
AATCATTTAC GTCAGCGGCA GCTCGACCAG CAGCGGCAGC AACAGCAACA GCAGCAGCAG 
CAGCGCAGTC TGGCCATGCG GGCGCAGGGC CTCACCATGC CACCCAACAT GGCGGCAGCC 
GCAGCGGGCA TGCCAGGAGC CATGAGCAAC CCGCGGATCC CCCAGGCCAA CCCTCAGCAG 
TTCCCCTACC CTCCCAACTA CGGTACAGGA CTGGCCTCGC CGCCCCCTTC CACCAGCCCT 
TTCTCCCCCG GTTCACCCAA CCTCCCTGCC CCTCAGCTGC TCTCCCACAG CGCCATGCAT 
GGCTCTCAGA TGAGTCTAGC TAACCAGGGG ATGCTGGGCA GTCTGAGCGG ACAGTTTGGG 
GCTGTTATGA GCCCTCAAAT GCAGCACAGT GCCTTTCAGT TCCCCAGCTC AGGTATGAGT 
CAGCAGTCAG ACGGTGGGTT TGGTGGTGCC GCGACCCCTC AGAGCCCCCT CATGTCACCC 
AGGATGGGTC ACGCTCAGAG TCCCATGATG CAACAGGCGC AGGGAAACAG CTCTTTCCAG 
TCGTCTCCTG ATATGAACGG CTGGCCGCAG GGCAACATCA ACACCAACAG TATGTTTACT 
CAGCAGTCGC CACCTCAGTT CAGCCAACAA GCCAACAACA ACATGTACAA CGGCAACGGC 
ATGAACCTCA ACGTGTCCAT GGCCGCCAAC TCCAGCAACA TGGGCCAAAT GGGCAACCAG 
ATGAGCATGA GCTCCATGAA CTCTGGGCCT GGGTCCGGCA TGGCCAACAT GGGCCCTGAA 
CAGAAGTATT GTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021325903.1
CDS	1..4515
Translation	MSAVGENSSDPARSEAQKRKEGPSDLLGPSPKRSTEKRNREHENKYIEELAELIFANFNDIDNFNVKPDKCAILKETVKQIRQIKEQEKAAAANEDEVQKADVSSTGQSVIDKDALGPMMLEALDGFFFVVNMEGNIVFVSENVTQYLRYNQEELMNTSVYSILHVGDHAEFIKNLLPKSHVNGVPWSSENPRRNSHTFNCRMLVNPHSEAEETQDHEAQQKYETMQCFAVSEPKSIKEEGEDFQSCLICVARRVPMKERPMLPTQESFTTRQDLQGKITSLDTSLLRASMKPGWEDLVRRCIQRFHLQNDGDISFAKRHQQEVIRHGQAFSPIYRFSLSDGTIVSAHTKSKLVRSSSTNEPQLYMSLHILQREQPVCGMASDLGNAQTMGKPMNPMSSPNTAGSSCTPQGQDATISSNTSTFPSPGAQKEPGAMHRFGCPGTMSHSATMQAATPQGSGYPLKLSSPSQGSPGMLSPRHRASPGVAGSPRLPPPQFSPAGSLHSPASMCTSSTGGNGGAGANHGYTSSSLNALQALSECHGVSHGQSLGSPDRKLGSPAAHSVAVSHHLINKMSVPESFGEQNQSEQGTPGQDEGIDLPRDEKGNLGQFGNLDGSDPQSRLRDNKSHTKLLQLLTTKTEPIESTSPPPMAGGEPGCKDGGAGNGGMGSQGGNGSHATSLKEKHKILHRLLQNSSSPVDLAKLTAEATGKELCQDSAGGTAGVPELAIKQEPVSPKKKDNALLRYLLDKDDNVLKGKGIKMEPGEIKEEGGKMTGIKTEKTDGGYDRVEPSSELDDILDDLQNSQPGLFTDSRPVSLPSAVDKQSIINDILQMTGESGANMSPQQQRALQTAVSQQNFPAPRPGQPGRAPPVRSVSLDMNMPPKGAQGQYPMMRNNNPYSIMQQQGMMGNHAVMPNQPNMVNAGVMGNNGPRVGMQQDGWGAQGTVGSAASPATAAPAGQHSLPQGALHSRMVPNPATGMPMRPNSQPGPRPMLQPQMMANAQSNMDMGMAGHQFPQQQAPPNQTAPWPDSVMPIEPVPFGNQSSSMYGGSQEDVLCPPASEGPADEGALLSQLYSALKDFDGLEEIDRALGIPALVGQAQPVEQDQFPGQDPSMMMDQKPPMYGQQQYASSPANMQQAGYTPMQDATFHGLQGQMGQRPGYPMLRMQARPGLRPGGVVPNQPNTLRLQLQHRLQAQQNRQPMMNQMGGVSNMNLPLRPNVPNQGTINAQMLAQRQRELLSNHLRQRQLDQQRQQQQQQQQQRSLAMRAQGLTMPPNMAAAAAGMPGAMSNPRIPQANPQQFPYPPNYGTGLASPPPSTSPFSPGSPNLPAPQLLSHSAMHGSQMSLANQGMLGSLSGQFGAVMSPQMQHSAFQFPSSGMSQQSDGGFGGAATPQSPLMSPRMGHAQSPMMQQAQGNSSFQSSPDMNGWPQGNINTNSMFTQQSPPQFSQQANNNMYNGNGMNLNVSMAANSSNMGQMGNQMSMSSMNSGPGSGMANMGPEQKYC

Target ORF information:

RefSeq Version	XM_021470228.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio nuclear receptor coactivator 2 (ncoa2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021470228.1

ORF Insert Sequence:

ATGAGTGCTG TCGGGGAAAA CTCGTCTGAC CCTGCCAGGT CAGAGGCTCA GAAGCGCAAG 
GAAGGCCCAT CAGACCTCCT GGGTCCAAGT CCCAAACGAA GTACAGAAAA ACGTAACCGG 
GAACATGAAA ATAAGTACAT TGAGGAGCTC GCCGAGCTGA TTTTCGCAAA CTTCAATGAC 
ATTGACAACT TCAACGTCAA GCCTGACAAA TGTGCAATCC TAAAGGAAAC CGTGAAACAG 
ATTCGGCAAA TTAAAGAGCA AGAGAAAGCT GCTGCAGCCA ATGAGGATGA AGTGCAGAAG 
GCTGATGTGT CGTCGACAGG CCAAAGTGTT ATTGACAAAG ATGCCCTTGG ACCAATGATG 
CTGGAGGCCT TGGATGGGTT CTTCTTTGTG GTGAACATGG AGGGCAACAT TGTGTTTGTA 
TCGGAGAATG TGACGCAGTA CCTGCGCTAT AATCAGGAAG AGCTGATGAA CACCAGTGTG 
TACAGCATAC TGCATGTAGG AGACCATGCC GAATTCATCA AAAACCTCCT GCCTAAGTCA 
CACGTGAATG GAGTGCCGTG GTCCAGCGAA AACCCTCGGC GGAACAGCCA CACCTTCAAC 
TGCCGCATGC TTGTGAACCC TCACAGCGAG GCCGAAGAGA CGCAGGATCA CGAGGCACAG 
CAGAAATATG AGACTATGCA GTGTTTTGCT GTGTCTGAAC CAAAGTCCAT CAAGGAGGAG 
GGAGAGGATT TCCAGTCGTG TCTGATCTGC GTGGCGCGGC GAGTGCCCAT GAAAGAGAGA 
CCGATGCTCC CCACGCAGGA GAGCTTCACC ACCCGCCAGG ACCTCCAAGG TAAAATCACG 
TCTCTGGACA CAAGTCTGTT ACGAGCGTCT ATGAAGCCAG GCTGGGAAGA CTTGGTGCGG 
AGATGCATTC AGCGTTTTCA TCTTCAGAAC GATGGAGACA TTTCCTTCGC CAAACGACAT 
CAGCAAGAAG TGATAAGGCA CGGGCAGGCG TTCAGTCCCA TCTACCGGTT CTCCCTGTCA 
GACGGGACCA TCGTCTCTGC TCACACCAAG AGCAAACTGG TTCGCTCATC CTCTACCAAC 
GAGCCACAGC TCTACATGTC CCTGCACATC TTACAGAGGG AGCAGCCCGT CTGTGGCATG 
GCCTCTGATC TTGGAAATGC TCAGACCATG GGAAAACCCA TGAACCCCAT GAGCTCTCCA 
AACACAGCGG GTTCCTCGTG CACTCCCCAA GGGCAGGACG CCACCATCAG CAGCAACACA 
TCCACTTTCC CCTCCCCTGG TGCCCAGAAG GAGCCTGGTG CCATGCACCG TTTCGGCTGC 
CCGGGAACCA TGAGCCATTC GGCAACCATG CAAGCGGCCA CCCCTCAGGG CAGCGGGTAT 
CCACTGAAGC TGAGCAGCCC GTCTCAGGGT AGTCCGGGCA TGCTTTCTCC ACGTCACCGA 
GCCAGTCCTG GAGTAGCAGG ATCTCCACGC TTACCCCCGC CACAGTTCTC TCCAGCGGGA 
AGCCTCCATT CACCTGCCAG CATGTGCACT AGCAGTACTG GTGGCAATGG GGGAGCAGGG 
GCCAACCATG GTTACACAAG CAGTTCACTT AATGCTCTTC AGGCGCTTAG CGAATGCCAT 
GGCGTAAGCC ACGGACAATC CCTGGGATCG CCTGATAGAA AGTTAGGGTC CCCAGCTGCA 
CATTCGGTAG CAGTAAGTCA TCACCTAATT AACAAAATGA GCGTACCTGA GTCATTCGGA 
GAGCAGAACC AATCAGAGCA GGGCACCCCT GGACAGGATG AAGGCATTGA TCTGCCAAGA 
GATGAAAAGG GCAACCTCGG GCAGTTCGGC AACCTGGACG GCAGTGATCC TCAAAGCCGA 
CTGCGCGACA ACAAAAGTCA CACTAAACTC CTGCAGCTCC TCACCACTAA AACTGAGCCT 
ATCGAGTCCA CTTCACCCCC TCCAATGGCC GGTGGAGAAC CTGGGTGTAA AGACGGCGGT 
GCAGGGAATG GTGGCATGGG TTCTCAAGGC GGAAACGGGT CACATGCTAC CTCCCTGAAG 
GAGAAACACA AAATCCTTCA CAGACTCCTC CAGAACAGCT CTTCGCCTGT TGATTTGGCC 
AAACTTACAG CTGAGGCCAC AGGGAAAGAG CTGTGTCAGG ATTCTGCTGG CGGGACGGCC 
GGTGTGCCCG AGTTGGCCAT TAAACAAGAG CCTGTCAGTC CCAAGAAAAA GGACAATGCC 
TTGCTGCGCT ACCTACTGGA TAAAGATGAC AATGTACTCA AGGGTAAAGG TATTAAAATG 
GAGCCTGGAG AGATCAAGGA GGAGGGGGGC AAGATGACTG GGATTAAGAC CGAGAAAACT 
GATGGTGGCT ACGACCGTGT GGAGCCGTCA AGTGAGCTGG ATGATATCCT GGATGATCTT 
CAGAACAGTC AGCCGGGCCT GTTCACTGAC TCCAGGCCTG TGTCTCTGCC TTCTGCTGTG 
GACAAGCAGT CAATCATCAA TGACATCCTG CAGATGACAG GAGAGAGCGG AGCTAACATG 
AGTCCTCAAC AGCAGAGAGC ACTGCAGACT GCAGTCAGCC AACAGAATTT CCCTGCTCCA 
CGGCCCGGTC AGCCTGGCAG AGCTCCTCCG GTCAGAAGCG TGTCTCTGGA CATGAACATG 
CCTCCTAAAG GCGCTCAGGG TCAGTATCCT ATGATGAGGA ACAACAACCC CTACTCAATC 
ATGCAGCAGC AAGGCATGAT GGGAAACCAC GCCGTCATGC CCAATCAGCC CAACATGGTG 
AACGCAGGAG TCATGGGTAA TAATGGTCCC CGTGTAGGCA TGCAGCAGGA CGGTTGGGGT 
GCTCAGGGTA CCGTCGGCAG CGCAGCTTCA CCTGCCACAG CTGCTCCCGC TGGCCAGCAC 
TCTCTCCCGC AGGGGGCACT GCACAGCCGC ATGGTGCCCA ACCCTGCCAC AGGAATGCCC 
ATGAGACCCA ACAGCCAGCC TGGACCCAGA CCAATGCTGC AACCGCAGAT GATGGCCAAT 
GCTCAGTCAA ACATGGACAT GGGCATGGCT GGCCACCAGT TTCCCCAGCA ACAGGCACCG 
CCCAATCAGA CGGCTCCATG GCCAGACAGT GTGATGCCTA TAGAGCCGGT GCCGTTTGGG 
AACCAGAGCA GTTCCATGTA CGGCGGGTCT CAGGAGGATG TGTTGTGCCC TCCTGCCTCT 
GAAGGTCCGG CTGATGAGGG GGCTTTACTC AGCCAGCTCT ACTCGGCCCT CAAAGATTTT 
GATGGTCTGG AGGAGATTGA CCGTGCGCTT GGCATTCCAG CGCTAGTGGG ACAGGCTCAG 
CCTGTGGAGC AGGATCAGTT CCCTGGTCAG GACCCTAGTA TGATGATGGA TCAGAAGCCA 
CCGATGTACG GGCAGCAACA GTATGCCTCG TCCCCGGCAA ACATGCAGCA GGCTGGATAC 
ACGCCAATGC AAGATGCCAC TTTCCACGGC TTGCAAGGAC AGATGGGACA GAGACCCGGA 
TACCCAATGC TCCGAATGCA AGCGAGACCG GGACTCAGAC CCGGTGGAGT CGTCCCAAAT 
CAACCCAATA CACTCAGACT GCAGCTTCAG CACAGGCTAC AAGCACAGCA GAATCGTCAG 
CCTATGATGA ATCAGATGGG TGGAGTGTCA AATATGAACC TACCTCTCAG ACCCAACGTT 
CCAAATCAGG GGACAATCAA CGCTCAGATG CTGGCCCAGC GGCAGAGAGA GTTGCTGAGT 
AATCATTTAC GTCAGCGGCA GCTCGACCAG CAGCGGCAGC AACAGCAACA GCAGCAGCAG 
CAGCGCAGTC TGGCCATGCG GGCGCAGGGC CTCACCATGC CACCCAACAT GGCGGCAGCC 
GCAGCGGGCA TGCCAGGAGC CATGAGCAAC CCGCGGATCC CCCAGGCCAA CCCTCAGCAG 
TTCCCCTACC CTCCCAACTA CGGTACAGGA CTGGCCTCGC CGCCCCCTTC CACCAGCCCT 
TTCTCCCCCG GTTCACCCAA CCTCCCTGCC CCTCAGCTGC TCTCCCACAG CGCCATGCAT 
GGCTCTCAGA TGAGTCTAGC TAACCAGGGG ATGCTGGGCA GTCTGAGCGG ACAGTTTGGG 
GCTGTTATGA GCCCTCAAAT GCAGCACAGT GCCTTTCAGT TCCCCAGCTC AGGTATGAGT 
CAGCAGTCAG ACGGTGGGTT TGGTGGTGCC GCGACCCCTC AGAGCCCCCT CATGTCACCC 
AGGATGGGTC ACGCTCAGAG TCCCATGATG CAACAGGCGC AGGGAAACAG CTCTTTCCAG 
TCGTCTCCTG ATATGAACGG CTGGCCGCAG GGCAACATCA ACACCAACAG TATGTTTACT 
CAGCAGTCGC CACCTCAGTT CAGCCAACAA GCCAACAACA ACATGTACAA CGGCAACGGC 
ATGAACCTCA ACGTGTCCAT GGCCGCCAAC TCCAGCAACA TGGGCCAAAT GGGCAACCAG 
ATGAGCATGA GCTCCATGAA CTCTGGGCCT GGGTCCGGCA TGGCCAACAT GGGCCCTGAA 
CAGAAGTATT GTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ncoa2 ( XM_009297338.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009295613.1 Danio rerio nuclear receptor coactivator 2 (ncoa2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa39442
Clone ID Related Accession (Same CDS sequence)	XM_009297338.2 , XM_009297338.3
Accession Version	XM_009297338.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4308bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	nuclear receptor coactivator 2 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007135.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009297338.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGCTG TCGGGGAAAA CTCGTCTGAC CCTGCCAGGT CAGAGGCTCA GAAGCGCAAG 
GAAGGCCCAT CAGACCTCCT GGGTCCAAGT CCCAAACGAA GTACAGAAAA ACGTAACCGG 
GAACATGAAA ATAAGTACAT TGAGGAGCTC GCCGAGCTGA TTTTCGCAAA CTTCAATGAC 
ATTGACAACT TCAACGTCAA GCCTGACAAA TGTGCAATCC TAAAGGAAAC CGTGAAACAG 
ATTCGGCAAA TTAAAGAGCA AGAGAAAGCT GCTGCAGCCA ATGAGGATGA AGTGCAGAAG 
GCTGATGTGT CGTCGACAGG CCAAAGTGTT ATTGACAAAG ATGCCCTTGG ACCAATGATG 
CTGGAGGCCT TGGATGGGTT CTTCTTTGTG GTGAACATGG AGGGCAACAT TGTGTTTGTA 
TCGGAGAATG TGACGCAGTA CCTGCGCTAT AATCAGGAAG AGCTGATGAA CACCAGTGTG 
TACAGCATAC TGCATGTAGG AGACCATGCC GAATTCATCA AAAACCTCCT GCCTAAGTCA 
CACGTGAATG GAGTGCCGTG GTCCAGCGAA AACCCTCGGC GGAACAGCCA CACCTTCAAC 
TGCCGCATGC TTGTGAACCC TCACAGCGAG GCCGAAGAGA CGCAGGATCA CGAGGCACAG 
CAGAAATATG AGACTATGCA GTGTTTTGCT GTGTCTGAAC CAAAGTCCAT CAAGGAGGAG 
GGAGAGGATT TCCAGTCGTG TCTGATCTGC GTGGCGCGGC GAGTGCCCAT GAAAGAGAGA 
CCGATGCTCC CCACGCAGGA GAGCTTCACC ACCCGCCAGG ACCTCCAAGG TAAAATCACG 
TCTCTGGACA CAAGTCTGTT ACGAGCGTCT ATGAAGCCAG GCTGGGAAGA CTTGGTGCGG 
AGATGCATTC AGCGTTTTCA TCTTCAGAAC GATGGAGACA TTTCCTTCGC CAAACGACAT 
CAGCAAGAAG TGATAAGGCA CGGGCAGGCG TTCAGTCCCA TCTACCGGTT CTCCCTGTCA 
GACGGGACCA TCGTCTCTGC TCACACCAAG AGCAAACTGG TTCGCTCATC CTCTACCAAC 
GAGCCACAGC TCTACATGTC CCTGCACATC TTACAGAGGG AGCAGCCCGT CTGTGGCATG 
GCCTCTGATC TTGGAAATGC TCAGACCATG GGAAAACCCA TGAACCCCAT GAGCTCTCCA 
AACACAGCGG GTTCCTCGTG CACTCCCCAA GGGCAGGACG CCACCATCAG CAGCAACACA 
TCCACTTTCC CCTCCCCTGG TGCCCAGAAG GAGCCTGGTG CCATGCACCG TTTCGGCTGC 
CCGGGAACCA TGAGCCATTC GGCAACCATG CAAGCGGCCA CCCCTCAGGG CAGCGGGTAT 
CCACTGAAGC TGAGCAGCCC GTCTCAGGGT AGTCCGGGCA TGCTTTCTCC ACGTCACCGA 
GCCAGTCCTG GAGTAGCAGG ATCTCCACGC TTACCCCCGC CACAGTTCTC TCCAGCGGGA 
AGCCTCCATT CACCTGCCAG CATGTGCACT AGCAGTACTG GTGGCAATGG GGGAGCAGGG 
GCCAACCATG GTTACACAAG CAGTTCACTT AATGCTCTTC AGGCGCTTAG CGAATGCCAT 
GGCGTAAGCC ACGGACAATC CCTGGGATCG CCTGATAGAA AGTTAGGGTC CCCAGCTGCA 
CATTCGGTAG CAGTAAGTCA TCACCTAATT AACAAAATGA GCGTACCTGA GTCATTCGGA 
GAGCAGAACC AATCAGAGCA GGGCACCCCT GGACAGGATG AAGGCATTGA TCTGCCAAGA 
GATGAAAAGG GCAACCTCGG GCAGTTCGGC AACCTGGACG GCAGTGATCC TCAAAGCCGA 
CTGCGCGACA ACAAAAGTCA CACTAAACTC CTGCAGCTCC TCACCACTAA AACTGAGCCT 
ATCGAGTCCA CTTCACCCCC TCCAATGGCC GGTGGAGAAC CTGGGTGTAA AGACGGCGGT 
GCAGGGAATG GTGGCATGGG TTCTCAAGGC GGAAACGGGT CACATGCTAC CTCCCTGAAG 
GAGAAACACA AAATCCTTCA CAGACTCCTC CAGAACAGCT CTTCGCCTGT TGATTTGGCC 
AAACTTACAG CTGAGGCCAC AGGGAAAGAG CTGTGTCAGG ATTCTGCTGG CGGGACGGCC 
GGTGTGCCCG AGTTGGCCAT TAAACAAGAG CCTGTCAGTC CCAAGAAAAA GGACAATGCC 
TTGCTGCGCT ACCTACTGGA TAAAGATGAC AATGTACTCA AGGGTAAAGG TATTAAAATG 
GAGCCTGGAG AGATCAAGGA GGAGGGGGGC AAGATGACTG GGATTAAGAC CGAGAAAACT 
GATGGTGGCT ACGACCGTGT GGAGCCGTCA AGTGAGCTGG ATGATATCCT GGATGATCTT 
CAGAACAGTC AGCCGGGCCT GTTCACTGAC TCCAGGCCTG TGTCTCTGCC TTCTGCTGTG 
GACAAGCAGT CAATCATCAA TGACATCCTG CAGATGACAG GAGAGAGCGG AGCTAACATG 
AGTCCTCAAC AGCAGAGAGC ACTGCAGACT GCAGTCAGCC AACAGAATTT CCCTGCTCCA 
CGGCCCGGTC AGCCTGGCAG AGCTCCTCCG GTCAGAAGCG TGTCTCTGGA CATGAACATG 
CCTCCTAAAG GCGCTCAGGG TCAGTATCCT ATGATGAGGA ACAACAACCC CTACTCAATC 
ATGCAGCAGC AAGGCATGAT GGGAAACCAC GCCGTCATGC CCAATCAGCC CAACATGGTG 
AACGCAGGAG TCATGGGTAA TAATGGTCCC CGTGTAGGCA TGCAGCAGGA CGGTTGGGGT 
GCTCAGGGTA CCGTCGGCAG CGCAGCTTCA CCTGCCACAG CTGCTCCCGC TGGCCAGCAC 
TCTCTCCCGC AGGGGGCACT GCACAGCCGC ATGGTGCCCA ACCCTGCCAC AGGAATGCCC 
ATGAGACCCA ACAGCCAGCC TGGACCCAGA CCAATGCTGC AACCGCAGAT GATGGCCAAT 
GCTCAGTCAA ACATGGACAT GGGCATGGCT GGCCACCAGT TTCCCCAGCA ACAGGCACCG 
CCCAATCAGA CGGCTCCATG GCCAGACAGT GTGATGCCTA TAGAGCCGGT GCCGTTTGGG 
AACCAGAGCA GTTCCATGTA CGGCGGGTCT CAGGAGGATG TGTTGTGCCC TCCTGCCTCT 
GAAGGTCCGG CTGATGAGGG GGCTTTACTC AGCCAGCTCT ACTCGGCCCT CAAAGATTTT 
GATGGTCTGG AGGAGATTGA CCGTGCGCTT GGCATTCCAG CGCTAGTGGG ACAGGCTCAG 
CCTGTGGAGC AGGATCAGTT CCCTGGTCAG GACCCTAGTA TGATGATGGA TCAGAAGCCA 
CCGATGTACG GGCAGCAACA GTATGCCTCG TCCCCGGCAA ACATGCAGCA GGCTGGATAC 
ACGCCAATGC AAGATGCCAC TTTCCACGGC TTGCAAGGAC AGATGGGACA GAGACCCGGA 
TACCCAATGC TCCGAATGCA AGCGAGACCG GGACTCAGAC CCGGTGGAGT CGTCCCAAAT 
CAACCCAATA CACTCAGACT GCAGCTTCAG CACAGGCTAC AAGCACAGCA GAATCGTCAG 
CCTATGATGA ATCAGATGGG TGGAGTGTCA AATATGAACC TACCTCTCAG ACCCAACGTT 
CCAAATCAGG GGACAATCAA CGCTCAGATG CTGGCCCAGC GGCAGAGAGA GTTGCTGAGT 
AATCATTTAC GTCAGCGGCA GCTCGACCAG CAGCGGCAGC AACAGCAACA GCAGCAGCAG 
CAGCGCAGTC TGGCCATGCG GGCGCAGGGC CTCACCATGC CACCCAACAT GGCGGCAGCC 
GCAGCGGGCA TGCCAGGAGC CATGAGCAAC CCGCGGATCC CCCAGGCCAA CCCTCAGCAG 
TTCCCCTACC CTCCCAACTA CGGTATGAGT CAGCAGTCAG ACGGTGGGTT TGGTGGTGCC 
GCGACCCCTC AGAGCCCCCT CATGTCACCC AGGATGGGTC ACGCTCAGAG TCCCATGATG 
CAACAGGCGC AGGGAAACAG CTCTTTCCAG TCGTCTCCTG ATATGAACGG CTGGCCGCAG 
GGCAACATCA ACACCAACAG TATGTTTACT CAGCAGTCGC CACCTCAGTT CAGCCAACAA 
GCCAACAACA ACATGTACAA CGGCAACGGC ATGAACCTCA ACGTGTCCAT GGCCGCCAAC 
TCCAGCAACA TGGGCCAAAT GGGCAACCAG ATGAGCATGA GCTCCATGAA CTCTGGGCCT 
GGGTCCGGCA TGGCCAACAT GGGCCCTGAA CAGCAGAAGT ATTGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009295613.1
CDS	1294..5601
Translation	MSAVGENSSDPARSEAQKRKEGPSDLLGPSPKRSTEKRNREHENKYIEELAELIFANFNDIDNFNVKPDKCAILKETVKQIRQIKEQEKAAAANEDEVQKADVSSTGQSVIDKDALGPMMLEALDGFFFVVNMEGNIVFVSENVTQYLRYNQEELMNTSVYSILHVGDHAEFIKNLLPKSHVNGVPWSSENPRRNSHTFNCRMLVNPHSEAEETQDHEAQQKYETMQCFAVSEPKSIKEEGEDFQSCLICVARRVPMKERPMLPTQESFTTRQDLQGKITSLDTSLLRASMKPGWEDLVRRCIQRFHLQNDGDISFAKRHQQEVIRHGQAFSPIYRFSLSDGTIVSAHTKSKLVRSSSTNEPQLYMSLHILQREQPVCGMASDLGNAQTMGKPMNPMSSPNTAGSSCTPQGQDATISSNTSTFPSPGAQKEPGAMHRFGCPGTMSHSATMQAATPQGSGYPLKLSSPSQGSPGMLSPRHRASPGVAGSPRLPPPQFSPAGSLHSPASMCTSSTGGNGGAGANHGYTSSSLNALQALSECHGVSHGQSLGSPDRKLGSPAAHSVAVSHHLINKMSVPESFGEQNQSEQGTPGQDEGIDLPRDEKGNLGQFGNLDGSDPQSRLRDNKSHTKLLQLLTTKTEPIESTSPPPMAGGEPGCKDGGAGNGGMGSQGGNGSHATSLKEKHKILHRLLQNSSSPVDLAKLTAEATGKELCQDSAGGTAGVPELAIKQEPVSPKKKDNALLRYLLDKDDNVLKGKGIKMEPGEIKEEGGKMTGIKTEKTDGGYDRVEPSSELDDILDDLQNSQPGLFTDSRPVSLPSAVDKQSIINDILQMTGESGANMSPQQQRALQTAVSQQNFPAPRPGQPGRAPPVRSVSLDMNMPPKGAQGQYPMMRNNNPYSIMQQQGMMGNHAVMPNQPNMVNAGVMGNNGPRVGMQQDGWGAQGTVGSAASPATAAPAGQHSLPQGALHSRMVPNPATGMPMRPNSQPGPRPMLQPQMMANAQSNMDMGMAGHQFPQQQAPPNQTAPWPDSVMPIEPVPFGNQSSSMYGGSQEDVLCPPASEGPADEGALLSQLYSALKDFDGLEEIDRALGIPALVGQAQPVEQDQFPGQDPSMMMDQKPPMYGQQQYASSPANMQQAGYTPMQDATFHGLQGQMGQRPGYPMLRMQARPGLRPGGVVPNQPNTLRLQLQHRLQAQQNRQPMMNQMGGVSNMNLPLRPNVPNQGTINAQMLAQRQRELLSNHLRQRQLDQQRQQQQQQQQQRSLAMRAQGLTMPPNMAAAAAGMPGAMSNPRIPQANPQQFPYPPNYGMSQQSDGGFGGAATPQSPLMSPRMGHAQSPMMQQAQGNSSFQSSPDMNGWPQGNINTNSMFTQQSPPQFSQQANNNMYNGNGMNLNVSMAANSSNMGQMGNQMSMSSMNSGPGSGMANMGPEQQKYC

Target ORF information:

RefSeq Version	XM_009297338.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio nuclear receptor coactivator 2 (ncoa2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009297338.2

ORF Insert Sequence:

ATGAGTGCTG TCGGGGAAAA CTCGTCTGAC CCTGCCAGGT CAGAGGCTCA GAAGCGCAAG 
GAAGGCCCAT CAGACCTCCT GGGTCCAAGT CCCAAACGAA GTACAGAAAA ACGTAACCGG 
GAACATGAAA ATAAGTACAT TGAGGAGCTC GCCGAGCTGA TTTTCGCAAA CTTCAATGAC 
ATTGACAACT TCAACGTCAA GCCTGACAAA TGTGCAATCC TAAAGGAAAC CGTGAAACAG 
ATTCGGCAAA TTAAAGAGCA AGAGAAAGCT GCTGCAGCCA ATGAGGATGA AGTGCAGAAG 
GCTGATGTGT CGTCGACAGG CCAAAGTGTT ATTGACAAAG ATGCCCTTGG ACCAATGATG 
CTGGAGGCCT TGGATGGGTT CTTCTTTGTG GTGAACATGG AGGGCAACAT TGTGTTTGTA 
TCGGAGAATG TGACGCAGTA CCTGCGCTAT AATCAGGAAG AGCTGATGAA CACCAGTGTG 
TACAGCATAC TGCATGTAGG AGACCATGCC GAATTCATCA AAAACCTCCT GCCTAAGTCA 
CACGTGAATG GAGTGCCGTG GTCCAGCGAA AACCCTCGGC GGAACAGCCA CACCTTCAAC 
TGCCGCATGC TTGTGAACCC TCACAGCGAG GCCGAAGAGA CGCAGGATCA CGAGGCACAG 
CAGAAATATG AGACTATGCA GTGTTTTGCT GTGTCTGAAC CAAAGTCCAT CAAGGAGGAG 
GGAGAGGATT TCCAGTCGTG TCTGATCTGC GTGGCGCGGC GAGTGCCCAT GAAAGAGAGA 
CCGATGCTCC CCACGCAGGA GAGCTTCACC ACCCGCCAGG ACCTCCAAGG TAAAATCACG 
TCTCTGGACA CAAGTCTGTT ACGAGCGTCT ATGAAGCCAG GCTGGGAAGA CTTGGTGCGG 
AGATGCATTC AGCGTTTTCA TCTTCAGAAC GATGGAGACA TTTCCTTCGC CAAACGACAT 
CAGCAAGAAG TGATAAGGCA CGGGCAGGCG TTCAGTCCCA TCTACCGGTT CTCCCTGTCA 
GACGGGACCA TCGTCTCTGC TCACACCAAG AGCAAACTGG TTCGCTCATC CTCTACCAAC 
GAGCCACAGC TCTACATGTC CCTGCACATC TTACAGAGGG AGCAGCCCGT CTGTGGCATG 
GCCTCTGATC TTGGAAATGC TCAGACCATG GGAAAACCCA TGAACCCCAT GAGCTCTCCA 
AACACAGCGG GTTCCTCGTG CACTCCCCAA GGGCAGGACG CCACCATCAG CAGCAACACA 
TCCACTTTCC CCTCCCCTGG TGCCCAGAAG GAGCCTGGTG CCATGCACCG TTTCGGCTGC 
CCGGGAACCA TGAGCCATTC GGCAACCATG CAAGCGGCCA CCCCTCAGGG CAGCGGGTAT 
CCACTGAAGC TGAGCAGCCC GTCTCAGGGT AGTCCGGGCA TGCTTTCTCC ACGTCACCGA 
GCCAGTCCTG GAGTAGCAGG ATCTCCACGC TTACCCCCGC CACAGTTCTC TCCAGCGGGA 
AGCCTCCATT CACCTGCCAG CATGTGCACT AGCAGTACTG GTGGCAATGG GGGAGCAGGG 
GCCAACCATG GTTACACAAG CAGTTCACTT AATGCTCTTC AGGCGCTTAG CGAATGCCAT 
GGCGTAAGCC ACGGACAATC CCTGGGATCG CCTGATAGAA AGTTAGGGTC CCCAGCTGCA 
CATTCGGTAG CAGTAAGTCA TCACCTAATT AACAAAATGA GCGTACCTGA GTCATTCGGA 
GAGCAGAACC AATCAGAGCA GGGCACCCCT GGACAGGATG AAGGCATTGA TCTGCCAAGA 
GATGAAAAGG GCAACCTCGG GCAGTTCGGC AACCTGGACG GCAGTGATCC TCAAAGCCGA 
CTGCGCGACA ACAAAAGTCA CACTAAACTC CTGCAGCTCC TCACCACTAA AACTGAGCCT 
ATCGAGTCCA CTTCACCCCC TCCAATGGCC GGTGGAGAAC CTGGGTGTAA AGACGGCGGT 
GCAGGGAATG GTGGCATGGG TTCTCAAGGC GGAAACGGGT CACATGCTAC CTCCCTGAAG 
GAGAAACACA AAATCCTTCA CAGACTCCTC CAGAACAGCT CTTCGCCTGT TGATTTGGCC 
AAACTTACAG CTGAGGCCAC AGGGAAAGAG CTGTGTCAGG ATTCTGCTGG CGGGACGGCC 
GGTGTGCCCG AGTTGGCCAT TAAACAAGAG CCTGTCAGTC CCAAGAAAAA GGACAATGCC 
TTGCTGCGCT ACCTACTGGA TAAAGATGAC AATGTACTCA AGGGTAAAGG TATTAAAATG 
GAGCCTGGAG AGATCAAGGA GGAGGGGGGC AAGATGACTG GGATTAAGAC CGAGAAAACT 
GATGGTGGCT ACGACCGTGT GGAGCCGTCA AGTGAGCTGG ATGATATCCT GGATGATCTT 
CAGAACAGTC AGCCGGGCCT GTTCACTGAC TCCAGGCCTG TGTCTCTGCC TTCTGCTGTG 
GACAAGCAGT CAATCATCAA TGACATCCTG CAGATGACAG GAGAGAGCGG AGCTAACATG 
AGTCCTCAAC AGCAGAGAGC ACTGCAGACT GCAGTCAGCC AACAGAATTT CCCTGCTCCA 
CGGCCCGGTC AGCCTGGCAG AGCTCCTCCG GTCAGAAGCG TGTCTCTGGA CATGAACATG 
CCTCCTAAAG GCGCTCAGGG TCAGTATCCT ATGATGAGGA ACAACAACCC CTACTCAATC 
ATGCAGCAGC AAGGCATGAT GGGAAACCAC GCCGTCATGC CCAATCAGCC CAACATGGTG 
AACGCAGGAG TCATGGGTAA TAATGGTCCC CGTGTAGGCA TGCAGCAGGA CGGTTGGGGT 
GCTCAGGGTA CCGTCGGCAG CGCAGCTTCA CCTGCCACAG CTGCTCCCGC TGGCCAGCAC 
TCTCTCCCGC AGGGGGCACT GCACAGCCGC ATGGTGCCCA ACCCTGCCAC AGGAATGCCC 
ATGAGACCCA ACAGCCAGCC TGGACCCAGA CCAATGCTGC AACCGCAGAT GATGGCCAAT 
GCTCAGTCAA ACATGGACAT GGGCATGGCT GGCCACCAGT TTCCCCAGCA ACAGGCACCG 
CCCAATCAGA CGGCTCCATG GCCAGACAGT GTGATGCCTA TAGAGCCGGT GCCGTTTGGG 
AACCAGAGCA GTTCCATGTA CGGCGGGTCT CAGGAGGATG TGTTGTGCCC TCCTGCCTCT 
GAAGGTCCGG CTGATGAGGG GGCTTTACTC AGCCAGCTCT ACTCGGCCCT CAAAGATTTT 
GATGGTCTGG AGGAGATTGA CCGTGCGCTT GGCATTCCAG CGCTAGTGGG ACAGGCTCAG 
CCTGTGGAGC AGGATCAGTT CCCTGGTCAG GACCCTAGTA TGATGATGGA TCAGAAGCCA 
CCGATGTACG GGCAGCAACA GTATGCCTCG TCCCCGGCAA ACATGCAGCA GGCTGGATAC 
ACGCCAATGC AAGATGCCAC TTTCCACGGC TTGCAAGGAC AGATGGGACA GAGACCCGGA 
TACCCAATGC TCCGAATGCA AGCGAGACCG GGACTCAGAC CCGGTGGAGT CGTCCCAAAT 
CAACCCAATA CACTCAGACT GCAGCTTCAG CACAGGCTAC AAGCACAGCA GAATCGTCAG 
CCTATGATGA ATCAGATGGG TGGAGTGTCA AATATGAACC TACCTCTCAG ACCCAACGTT 
CCAAATCAGG GGACAATCAA CGCTCAGATG CTGGCCCAGC GGCAGAGAGA GTTGCTGAGT 
AATCATTTAC GTCAGCGGCA GCTCGACCAG CAGCGGCAGC AACAGCAACA GCAGCAGCAG 
CAGCGCAGTC TGGCCATGCG GGCGCAGGGC CTCACCATGC CACCCAACAT GGCGGCAGCC 
GCAGCGGGCA TGCCAGGAGC CATGAGCAAC CCGCGGATCC CCCAGGCCAA CCCTCAGCAG 
TTCCCCTACC CTCCCAACTA CGGTATGAGT CAGCAGTCAG ACGGTGGGTT TGGTGGTGCC 
GCGACCCCTC AGAGCCCCCT CATGTCACCC AGGATGGGTC ACGCTCAGAG TCCCATGATG 
CAACAGGCGC AGGGAAACAG CTCTTTCCAG TCGTCTCCTG ATATGAACGG CTGGCCGCAG 
GGCAACATCA ACACCAACAG TATGTTTACT CAGCAGTCGC CACCTCAGTT CAGCCAACAA 
GCCAACAACA ACATGTACAA CGGCAACGGC ATGAACCTCA ACGTGTCCAT GGCCGCCAAC 
TCCAGCAACA TGGGCCAAAT GGGCAACCAG ATGAGCATGA GCTCCATGAA CTCTGGGCCT 
GGGTCCGGCA TGGCCAACAT GGGCCCTGAA CAGCAGAAGT ATTGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ncoa2 ( XM_009297338.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009295613.1 Danio rerio nuclear receptor coactivator 2 (ncoa2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa39442
Clone ID Related Accession (Same CDS sequence)	XM_009297338.2 , XM_009297338.3
Accession Version	XM_009297338.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4308bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	nuclear receptor coactivator 2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007135.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009297338.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGCTG TCGGGGAAAA CTCGTCTGAC CCTGCCAGGT CAGAGGCTCA GAAGCGCAAG 
GAAGGCCCAT CAGACCTCCT GGGTCCAAGT CCCAAACGAA GTACAGAAAA ACGTAACCGG 
GAACATGAAA ATAAGTACAT TGAGGAGCTC GCCGAGCTGA TTTTCGCAAA CTTCAATGAC 
ATTGACAACT TCAACGTCAA GCCTGACAAA TGTGCAATCC TAAAGGAAAC CGTGAAACAG 
ATTCGGCAAA TTAAAGAGCA AGAGAAAGCT GCTGCAGCCA ATGAGGATGA AGTGCAGAAG 
GCTGATGTGT CGTCGACAGG CCAAAGTGTT ATTGACAAAG ATGCCCTTGG ACCAATGATG 
CTGGAGGCCT TGGATGGGTT CTTCTTTGTG GTGAACATGG AGGGCAACAT TGTGTTTGTA 
TCGGAGAATG TGACGCAGTA CCTGCGCTAT AATCAGGAAG AGCTGATGAA CACCAGTGTG 
TACAGCATAC TGCATGTAGG AGACCATGCC GAATTCATCA AAAACCTCCT GCCTAAGTCA 
CACGTGAATG GAGTGCCGTG GTCCAGCGAA AACCCTCGGC GGAACAGCCA CACCTTCAAC 
TGCCGCATGC TTGTGAACCC TCACAGCGAG GCCGAAGAGA CGCAGGATCA CGAGGCACAG 
CAGAAATATG AGACTATGCA GTGTTTTGCT GTGTCTGAAC CAAAGTCCAT CAAGGAGGAG 
GGAGAGGATT TCCAGTCGTG TCTGATCTGC GTGGCGCGGC GAGTGCCCAT GAAAGAGAGA 
CCGATGCTCC CCACGCAGGA GAGCTTCACC ACCCGCCAGG ACCTCCAAGG TAAAATCACG 
TCTCTGGACA CAAGTCTGTT ACGAGCGTCT ATGAAGCCAG GCTGGGAAGA CTTGGTGCGG 
AGATGCATTC AGCGTTTTCA TCTTCAGAAC GATGGAGACA TTTCCTTCGC CAAACGACAT 
CAGCAAGAAG TGATAAGGCA CGGGCAGGCG TTCAGTCCCA TCTACCGGTT CTCCCTGTCA 
GACGGGACCA TCGTCTCTGC TCACACCAAG AGCAAACTGG TTCGCTCATC CTCTACCAAC 
GAGCCACAGC TCTACATGTC CCTGCACATC TTACAGAGGG AGCAGCCCGT CTGTGGCATG 
GCCTCTGATC TTGGAAATGC TCAGACCATG GGAAAACCCA TGAACCCCAT GAGCTCTCCA 
AACACAGCGG GTTCCTCGTG CACTCCCCAA GGGCAGGACG CCACCATCAG CAGCAACACA 
TCCACTTTCC CCTCCCCTGG TGCCCAGAAG GAGCCTGGTG CCATGCACCG TTTCGGCTGC 
CCGGGAACCA TGAGCCATTC GGCAACCATG CAAGCGGCCA CCCCTCAGGG CAGCGGGTAT 
CCACTGAAGC TGAGCAGCCC GTCTCAGGGT AGTCCGGGCA TGCTTTCTCC ACGTCACCGA 
GCCAGTCCTG GAGTAGCAGG ATCTCCACGC TTACCCCCGC CACAGTTCTC TCCAGCGGGA 
AGCCTCCATT CACCTGCCAG CATGTGCACT AGCAGTACTG GTGGCAATGG GGGAGCAGGG 
GCCAACCATG GTTACACAAG CAGTTCACTT AATGCTCTTC AGGCGCTTAG CGAATGCCAT 
GGCGTAAGCC ACGGACAATC CCTGGGATCG CCTGATAGAA AGTTAGGGTC CCCAGCTGCA 
CATTCGGTAG CAGTAAGTCA TCACCTAATT AACAAAATGA GCGTACCTGA GTCATTCGGA 
GAGCAGAACC AATCAGAGCA GGGCACCCCT GGACAGGATG AAGGCATTGA TCTGCCAAGA 
GATGAAAAGG GCAACCTCGG GCAGTTCGGC AACCTGGACG GCAGTGATCC TCAAAGCCGA 
CTGCGCGACA ACAAAAGTCA CACTAAACTC CTGCAGCTCC TCACCACTAA AACTGAGCCT 
ATCGAGTCCA CTTCACCCCC TCCAATGGCC GGTGGAGAAC CTGGGTGTAA AGACGGCGGT 
GCAGGGAATG GTGGCATGGG TTCTCAAGGC GGAAACGGGT CACATGCTAC CTCCCTGAAG 
GAGAAACACA AAATCCTTCA CAGACTCCTC CAGAACAGCT CTTCGCCTGT TGATTTGGCC 
AAACTTACAG CTGAGGCCAC AGGGAAAGAG CTGTGTCAGG ATTCTGCTGG CGGGACGGCC 
GGTGTGCCCG AGTTGGCCAT TAAACAAGAG CCTGTCAGTC CCAAGAAAAA GGACAATGCC 
TTGCTGCGCT ACCTACTGGA TAAAGATGAC AATGTACTCA AGGGTAAAGG TATTAAAATG 
GAGCCTGGAG AGATCAAGGA GGAGGGGGGC AAGATGACTG GGATTAAGAC CGAGAAAACT 
GATGGTGGCT ACGACCGTGT GGAGCCGTCA AGTGAGCTGG ATGATATCCT GGATGATCTT 
CAGAACAGTC AGCCGGGCCT GTTCACTGAC TCCAGGCCTG TGTCTCTGCC TTCTGCTGTG 
GACAAGCAGT CAATCATCAA TGACATCCTG CAGATGACAG GAGAGAGCGG AGCTAACATG 
AGTCCTCAAC AGCAGAGAGC ACTGCAGACT GCAGTCAGCC AACAGAATTT CCCTGCTCCA 
CGGCCCGGTC AGCCTGGCAG AGCTCCTCCG GTCAGAAGCG TGTCTCTGGA CATGAACATG 
CCTCCTAAAG GCGCTCAGGG TCAGTATCCT ATGATGAGGA ACAACAACCC CTACTCAATC 
ATGCAGCAGC AAGGCATGAT GGGAAACCAC GCCGTCATGC CCAATCAGCC CAACATGGTG 
AACGCAGGAG TCATGGGTAA TAATGGTCCC CGTGTAGGCA TGCAGCAGGA CGGTTGGGGT 
GCTCAGGGTA CCGTCGGCAG CGCAGCTTCA CCTGCCACAG CTGCTCCCGC TGGCCAGCAC 
TCTCTCCCGC AGGGGGCACT GCACAGCCGC ATGGTGCCCA ACCCTGCCAC AGGAATGCCC 
ATGAGACCCA ACAGCCAGCC TGGACCCAGA CCAATGCTGC AACCGCAGAT GATGGCCAAT 
GCTCAGTCAA ACATGGACAT GGGCATGGCT GGCCACCAGT TTCCCCAGCA ACAGGCACCG 
CCCAATCAGA CGGCTCCATG GCCAGACAGT GTGATGCCTA TAGAGCCGGT GCCGTTTGGG 
AACCAGAGCA GTTCCATGTA CGGCGGGTCT CAGGAGGATG TGTTGTGCCC TCCTGCCTCT 
GAAGGTCCGG CTGATGAGGG GGCTTTACTC AGCCAGCTCT ACTCGGCCCT CAAAGATTTT 
GATGGTCTGG AGGAGATTGA CCGTGCGCTT GGCATTCCAG CGCTAGTGGG ACAGGCTCAG 
CCTGTGGAGC AGGATCAGTT CCCTGGTCAG GACCCTAGTA TGATGATGGA TCAGAAGCCA 
CCGATGTACG GGCAGCAACA GTATGCCTCG TCCCCGGCAA ACATGCAGCA GGCTGGATAC 
ACGCCAATGC AAGATGCCAC TTTCCACGGC TTGCAAGGAC AGATGGGACA GAGACCCGGA 
TACCCAATGC TCCGAATGCA AGCGAGACCG GGACTCAGAC CCGGTGGAGT CGTCCCAAAT 
CAACCCAATA CACTCAGACT GCAGCTTCAG CACAGGCTAC AAGCACAGCA GAATCGTCAG 
CCTATGATGA ATCAGATGGG TGGAGTGTCA AATATGAACC TACCTCTCAG ACCCAACGTT 
CCAAATCAGG GGACAATCAA CGCTCAGATG CTGGCCCAGC GGCAGAGAGA GTTGCTGAGT 
AATCATTTAC GTCAGCGGCA GCTCGACCAG CAGCGGCAGC AACAGCAACA GCAGCAGCAG 
CAGCGCAGTC TGGCCATGCG GGCGCAGGGC CTCACCATGC CACCCAACAT GGCGGCAGCC 
GCAGCGGGCA TGCCAGGAGC CATGAGCAAC CCGCGGATCC CCCAGGCCAA CCCTCAGCAG 
TTCCCCTACC CTCCCAACTA CGGTATGAGT CAGCAGTCAG ACGGTGGGTT TGGTGGTGCC 
GCGACCCCTC AGAGCCCCCT CATGTCACCC AGGATGGGTC ACGCTCAGAG TCCCATGATG 
CAACAGGCGC AGGGAAACAG CTCTTTCCAG TCGTCTCCTG ATATGAACGG CTGGCCGCAG 
GGCAACATCA ACACCAACAG TATGTTTACT CAGCAGTCGC CACCTCAGTT CAGCCAACAA 
GCCAACAACA ACATGTACAA CGGCAACGGC ATGAACCTCA ACGTGTCCAT GGCCGCCAAC 
TCCAGCAACA TGGGCCAAAT GGGCAACCAG ATGAGCATGA GCTCCATGAA CTCTGGGCCT 
GGGTCCGGCA TGGCCAACAT GGGCCCTGAA CAGCAGAAGT ATTGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009295613.1
CDS	322..4629
Translation	MSAVGENSSDPARSEAQKRKEGPSDLLGPSPKRSTEKRNREHENKYIEELAELIFANFNDIDNFNVKPDKCAILKETVKQIRQIKEQEKAAAANEDEVQKADVSSTGQSVIDKDALGPMMLEALDGFFFVVNMEGNIVFVSENVTQYLRYNQEELMNTSVYSILHVGDHAEFIKNLLPKSHVNGVPWSSENPRRNSHTFNCRMLVNPHSEAEETQDHEAQQKYETMQCFAVSEPKSIKEEGEDFQSCLICVARRVPMKERPMLPTQESFTTRQDLQGKITSLDTSLLRASMKPGWEDLVRRCIQRFHLQNDGDISFAKRHQQEVIRHGQAFSPIYRFSLSDGTIVSAHTKSKLVRSSSTNEPQLYMSLHILQREQPVCGMASDLGNAQTMGKPMNPMSSPNTAGSSCTPQGQDATISSNTSTFPSPGAQKEPGAMHRFGCPGTMSHSATMQAATPQGSGYPLKLSSPSQGSPGMLSPRHRASPGVAGSPRLPPPQFSPAGSLHSPASMCTSSTGGNGGAGANHGYTSSSLNALQALSECHGVSHGQSLGSPDRKLGSPAAHSVAVSHHLINKMSVPESFGEQNQSEQGTPGQDEGIDLPRDEKGNLGQFGNLDGSDPQSRLRDNKSHTKLLQLLTTKTEPIESTSPPPMAGGEPGCKDGGAGNGGMGSQGGNGSHATSLKEKHKILHRLLQNSSSPVDLAKLTAEATGKELCQDSAGGTAGVPELAIKQEPVSPKKKDNALLRYLLDKDDNVLKGKGIKMEPGEIKEEGGKMTGIKTEKTDGGYDRVEPSSELDDILDDLQNSQPGLFTDSRPVSLPSAVDKQSIINDILQMTGESGANMSPQQQRALQTAVSQQNFPAPRPGQPGRAPPVRSVSLDMNMPPKGAQGQYPMMRNNNPYSIMQQQGMMGNHAVMPNQPNMVNAGVMGNNGPRVGMQQDGWGAQGTVGSAASPATAAPAGQHSLPQGALHSRMVPNPATGMPMRPNSQPGPRPMLQPQMMANAQSNMDMGMAGHQFPQQQAPPNQTAPWPDSVMPIEPVPFGNQSSSMYGGSQEDVLCPPASEGPADEGALLSQLYSALKDFDGLEEIDRALGIPALVGQAQPVEQDQFPGQDPSMMMDQKPPMYGQQQYASSPANMQQAGYTPMQDATFHGLQGQMGQRPGYPMLRMQARPGLRPGGVVPNQPNTLRLQLQHRLQAQQNRQPMMNQMGGVSNMNLPLRPNVPNQGTINAQMLAQRQRELLSNHLRQRQLDQQRQQQQQQQQQRSLAMRAQGLTMPPNMAAAAAGMPGAMSNPRIPQANPQQFPYPPNYGMSQQSDGGFGGAATPQSPLMSPRMGHAQSPMMQQAQGNSSFQSSPDMNGWPQGNINTNSMFTQQSPPQFSQQANNNMYNGNGMNLNVSMAANSSNMGQMGNQMSMSSMNSGPGSGMANMGPEQQKYC

Target ORF information:

RefSeq Version	XM_009297338.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio nuclear receptor coactivator 2 (ncoa2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009297338.3

ORF Insert Sequence:

ATGAGTGCTG TCGGGGAAAA CTCGTCTGAC CCTGCCAGGT CAGAGGCTCA GAAGCGCAAG 
GAAGGCCCAT CAGACCTCCT GGGTCCAAGT CCCAAACGAA GTACAGAAAA ACGTAACCGG 
GAACATGAAA ATAAGTACAT TGAGGAGCTC GCCGAGCTGA TTTTCGCAAA CTTCAATGAC 
ATTGACAACT TCAACGTCAA GCCTGACAAA TGTGCAATCC TAAAGGAAAC CGTGAAACAG 
ATTCGGCAAA TTAAAGAGCA AGAGAAAGCT GCTGCAGCCA ATGAGGATGA AGTGCAGAAG 
GCTGATGTGT CGTCGACAGG CCAAAGTGTT ATTGACAAAG ATGCCCTTGG ACCAATGATG 
CTGGAGGCCT TGGATGGGTT CTTCTTTGTG GTGAACATGG AGGGCAACAT TGTGTTTGTA 
TCGGAGAATG TGACGCAGTA CCTGCGCTAT AATCAGGAAG AGCTGATGAA CACCAGTGTG 
TACAGCATAC TGCATGTAGG AGACCATGCC GAATTCATCA AAAACCTCCT GCCTAAGTCA 
CACGTGAATG GAGTGCCGTG GTCCAGCGAA AACCCTCGGC GGAACAGCCA CACCTTCAAC 
TGCCGCATGC TTGTGAACCC TCACAGCGAG GCCGAAGAGA CGCAGGATCA CGAGGCACAG 
CAGAAATATG AGACTATGCA GTGTTTTGCT GTGTCTGAAC CAAAGTCCAT CAAGGAGGAG 
GGAGAGGATT TCCAGTCGTG TCTGATCTGC GTGGCGCGGC GAGTGCCCAT GAAAGAGAGA 
CCGATGCTCC CCACGCAGGA GAGCTTCACC ACCCGCCAGG ACCTCCAAGG TAAAATCACG 
TCTCTGGACA CAAGTCTGTT ACGAGCGTCT ATGAAGCCAG GCTGGGAAGA CTTGGTGCGG 
AGATGCATTC AGCGTTTTCA TCTTCAGAAC GATGGAGACA TTTCCTTCGC CAAACGACAT 
CAGCAAGAAG TGATAAGGCA CGGGCAGGCG TTCAGTCCCA TCTACCGGTT CTCCCTGTCA 
GACGGGACCA TCGTCTCTGC TCACACCAAG AGCAAACTGG TTCGCTCATC CTCTACCAAC 
GAGCCACAGC TCTACATGTC CCTGCACATC TTACAGAGGG AGCAGCCCGT CTGTGGCATG 
GCCTCTGATC TTGGAAATGC TCAGACCATG GGAAAACCCA TGAACCCCAT GAGCTCTCCA 
AACACAGCGG GTTCCTCGTG CACTCCCCAA GGGCAGGACG CCACCATCAG CAGCAACACA 
TCCACTTTCC CCTCCCCTGG TGCCCAGAAG GAGCCTGGTG CCATGCACCG TTTCGGCTGC 
CCGGGAACCA TGAGCCATTC GGCAACCATG CAAGCGGCCA CCCCTCAGGG CAGCGGGTAT 
CCACTGAAGC TGAGCAGCCC GTCTCAGGGT AGTCCGGGCA TGCTTTCTCC ACGTCACCGA 
GCCAGTCCTG GAGTAGCAGG ATCTCCACGC TTACCCCCGC CACAGTTCTC TCCAGCGGGA 
AGCCTCCATT CACCTGCCAG CATGTGCACT AGCAGTACTG GTGGCAATGG GGGAGCAGGG 
GCCAACCATG GTTACACAAG CAGTTCACTT AATGCTCTTC AGGCGCTTAG CGAATGCCAT 
GGCGTAAGCC ACGGACAATC CCTGGGATCG CCTGATAGAA AGTTAGGGTC CCCAGCTGCA 
CATTCGGTAG CAGTAAGTCA TCACCTAATT AACAAAATGA GCGTACCTGA GTCATTCGGA 
GAGCAGAACC AATCAGAGCA GGGCACCCCT GGACAGGATG AAGGCATTGA TCTGCCAAGA 
GATGAAAAGG GCAACCTCGG GCAGTTCGGC AACCTGGACG GCAGTGATCC TCAAAGCCGA 
CTGCGCGACA ACAAAAGTCA CACTAAACTC CTGCAGCTCC TCACCACTAA AACTGAGCCT 
ATCGAGTCCA CTTCACCCCC TCCAATGGCC GGTGGAGAAC CTGGGTGTAA AGACGGCGGT 
GCAGGGAATG GTGGCATGGG TTCTCAAGGC GGAAACGGGT CACATGCTAC CTCCCTGAAG 
GAGAAACACA AAATCCTTCA CAGACTCCTC CAGAACAGCT CTTCGCCTGT TGATTTGGCC 
AAACTTACAG CTGAGGCCAC AGGGAAAGAG CTGTGTCAGG ATTCTGCTGG CGGGACGGCC 
GGTGTGCCCG AGTTGGCCAT TAAACAAGAG CCTGTCAGTC CCAAGAAAAA GGACAATGCC 
TTGCTGCGCT ACCTACTGGA TAAAGATGAC AATGTACTCA AGGGTAAAGG TATTAAAATG 
GAGCCTGGAG AGATCAAGGA GGAGGGGGGC AAGATGACTG GGATTAAGAC CGAGAAAACT 
GATGGTGGCT ACGACCGTGT GGAGCCGTCA AGTGAGCTGG ATGATATCCT GGATGATCTT 
CAGAACAGTC AGCCGGGCCT GTTCACTGAC TCCAGGCCTG TGTCTCTGCC TTCTGCTGTG 
GACAAGCAGT CAATCATCAA TGACATCCTG CAGATGACAG GAGAGAGCGG AGCTAACATG 
AGTCCTCAAC AGCAGAGAGC ACTGCAGACT GCAGTCAGCC AACAGAATTT CCCTGCTCCA 
CGGCCCGGTC AGCCTGGCAG AGCTCCTCCG GTCAGAAGCG TGTCTCTGGA CATGAACATG 
CCTCCTAAAG GCGCTCAGGG TCAGTATCCT ATGATGAGGA ACAACAACCC CTACTCAATC 
ATGCAGCAGC AAGGCATGAT GGGAAACCAC GCCGTCATGC CCAATCAGCC CAACATGGTG 
AACGCAGGAG TCATGGGTAA TAATGGTCCC CGTGTAGGCA TGCAGCAGGA CGGTTGGGGT 
GCTCAGGGTA CCGTCGGCAG CGCAGCTTCA CCTGCCACAG CTGCTCCCGC TGGCCAGCAC 
TCTCTCCCGC AGGGGGCACT GCACAGCCGC ATGGTGCCCA ACCCTGCCAC AGGAATGCCC 
ATGAGACCCA ACAGCCAGCC TGGACCCAGA CCAATGCTGC AACCGCAGAT GATGGCCAAT 
GCTCAGTCAA ACATGGACAT GGGCATGGCT GGCCACCAGT TTCCCCAGCA ACAGGCACCG 
CCCAATCAGA CGGCTCCATG GCCAGACAGT GTGATGCCTA TAGAGCCGGT GCCGTTTGGG 
AACCAGAGCA GTTCCATGTA CGGCGGGTCT CAGGAGGATG TGTTGTGCCC TCCTGCCTCT 
GAAGGTCCGG CTGATGAGGG GGCTTTACTC AGCCAGCTCT ACTCGGCCCT CAAAGATTTT 
GATGGTCTGG AGGAGATTGA CCGTGCGCTT GGCATTCCAG CGCTAGTGGG ACAGGCTCAG 
CCTGTGGAGC AGGATCAGTT CCCTGGTCAG GACCCTAGTA TGATGATGGA TCAGAAGCCA 
CCGATGTACG GGCAGCAACA GTATGCCTCG TCCCCGGCAA ACATGCAGCA GGCTGGATAC 
ACGCCAATGC AAGATGCCAC TTTCCACGGC TTGCAAGGAC AGATGGGACA GAGACCCGGA 
TACCCAATGC TCCGAATGCA AGCGAGACCG GGACTCAGAC CCGGTGGAGT CGTCCCAAAT 
CAACCCAATA CACTCAGACT GCAGCTTCAG CACAGGCTAC AAGCACAGCA GAATCGTCAG 
CCTATGATGA ATCAGATGGG TGGAGTGTCA AATATGAACC TACCTCTCAG ACCCAACGTT 
CCAAATCAGG GGACAATCAA CGCTCAGATG CTGGCCCAGC GGCAGAGAGA GTTGCTGAGT 
AATCATTTAC GTCAGCGGCA GCTCGACCAG CAGCGGCAGC AACAGCAACA GCAGCAGCAG 
CAGCGCAGTC TGGCCATGCG GGCGCAGGGC CTCACCATGC CACCCAACAT GGCGGCAGCC 
GCAGCGGGCA TGCCAGGAGC CATGAGCAAC CCGCGGATCC CCCAGGCCAA CCCTCAGCAG 
TTCCCCTACC CTCCCAACTA CGGTATGAGT CAGCAGTCAG ACGGTGGGTT TGGTGGTGCC 
GCGACCCCTC AGAGCCCCCT CATGTCACCC AGGATGGGTC ACGCTCAGAG TCCCATGATG 
CAACAGGCGC AGGGAAACAG CTCTTTCCAG TCGTCTCCTG ATATGAACGG CTGGCCGCAG 
GGCAACATCA ACACCAACAG TATGTTTACT CAGCAGTCGC CACCTCAGTT CAGCCAACAA 
GCCAACAACA ACATGTACAA CGGCAACGGC ATGAACCTCA ACGTGTCCAT GGCCGCCAAC 
TCCAGCAACA TGGGCCAAAT GGGCAACCAG ATGAGCATGA GCTCCATGAA CTCTGGGCCT 
GGGTCCGGCA TGGCCAACAT GGGCCCTGAA CAGCAGAAGT ATTGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Pharmacokinetics and effects of tetrabromobisphenol a (TBBPA) to early life stages of zebrafish (Danio rerio).
Chemosphere190243-252(2018 Jan)
Liu H,Ma Z,Zhang T,Yu N,Su G,Giesy JP,Yu H

Integrated in silico and in vivo approaches to investigate effects of BDE-99 mediated by the nuclear receptors on developing zebrafish.
Environmental toxicology and chemistry(2017 Oct)
Zhang L,Jin Y,Han Z,Liu H,Shi L,Hua X,Doering JA,Tang S,Giesy JP,Yu H

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ncoa2 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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