tyms cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	tyms
Entrez Gene ID	81540
Full Name	thymidylate synthetase
Synonyms	zgc:56465,zgc:77659
General protein information	Preferred Names thymidylate synthetase Names thymidylate synthase
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_131760.2	NP_571835.2	thymidylate synthase

Pathways from BioSystems

Homologies

Homo sapiens (human)	TYMS	NP_001062.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010457	XP_311491.3
Arabidopsis thaliana (thale cress)	AT2G21550	NP_179750.1
Canis lupus familiaris (dog)	TYMS	NP_001239103.1
Saccharomyces cerevisiae (baker's yeast)	CDC21	NP_014717.2
Eremothecium gossypii	AGOS_ADR219C	NP_984315.1
Magnaporthe oryzae (rice blast fungus)	MGG_04458	XP_003713554.1
Arabidopsis thaliana (thale cress)	THY-2	NP_195183.2
Xenopus tropicalis (tropical clawed frog)	tyms	NP_001072852.1
Rattus norvegicus (Norway rat)	Tyms	NP_062052.1
Gallus gallus (chicken)	TYMS	XP_419147.3
Drosophila melanogaster (fruit fly)	Ts	NP_477367.1
Schizosaccharomyces pombe (fission yeast)	SPAC15E1.04	NP_594305.1
Mus musculus (house mouse)	Tyms	NP_067263.1
Caenorhabditis elegans (roundworm)	Y110A7A.4	NP_491532.3
Neurospora crassa	NCU10053	XP_957941.1
Pan troglodytes (chimpanzee)	TYMS	NP_001233511.1
	TYMS	XP_001089274.1
Bos taurus (cattle)	TYMS	NP_001032905.1
Danio rerio (zebrafish)	tyms	NP_571835.1
Kluyveromyces lactis	KLLA0A07843g	XP_451349.1
Arabidopsis thaliana (thale cress)	THY-1	NP_179230.1

Gene Ontology
Bibliography

Related articles in PubMed

Dynamic phosphorylation of Histone Deacetylase 1 by Aurora kinases during mitosis regulates zebrafish embryos development.
Loponte S, Segr? CV, Senese S, Miccolo C, Santaguida S, Deflorian G, Citro S, Mattoscio D, Pisati F, Moser MA, Visintin R, Seiser C, Chiocca S
Scientific reports630213(2016 Jul)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase (cad) regulates Notch signaling and vascular development in zebrafish.
Coxam B, Neyt C, Grassini DR, Le Guen L, Smith KA, Schulte-Merker S, Hogan BM
Developmental dynamics : an official publication of the American Association of Anatomists244(1)1-9(2015 Jan)

Zebrafish midbrain slow-amplifying progenitors exhibit high levels of transcripts for nucleotide and ribosome biogenesis.
Recher G, Jouralet J, Brombin A, Heuz? A, Mugniery E, Hermel JM, Desnoulez S, Savy T, Herbomel P, Bourrat F, Peyri?ras N, Jamen F, Joly JS
Development (Cambridge, England)140(24)4860-9(2013 Dec)

Regulation of E2F1 by the von Hippel-Lindau tumour suppressor protein predicts survival in renal cell cancer patients.
Mans DA, Vermaat JS, Weijts BG, van Rooijen E, van Reeuwijk J, Boldt K, Daenen LG, van der Groep P, Rowland BD, Jans JJ, Roepman R, Voest EE, van Diest PJ, Verhaar MC, de Bruin A, Giles RH
The Journal of pathology231(1)117-29(2013 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

This study revealed a structural requirement for the interaction of TS RNA with TS protein.
Title: Interaction between thymidylate synthase and its cognate mRNA in zebrafish embryos.

The following tyms gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the tyms cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa53179	NM_131760.2 Latest version!	Danio rerio thymidylate synthetase (tyms), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$174.30 $249.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

tyms ( NM_131760.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_571835.2 Danio rerio thymidylate synthetase (tyms), mRNA.

Price & Availability↑

CloneID	ODa53179
Clone ID Related Accession (Same CDS sequence)	NM_131760.2
Accession Version	NM_131760.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 957bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	thymidylate synthase
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from FP016237.8, EH580067.1 and EH577904.1. On Sep 1, 2015 this sequence version replaced NM_131760.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY005804.1, GDQH01021766.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCCGACA CGGCAGTGGA AGTGACAAAT GGACATTGCG TTAACGGAGA AAGCAAGAAA 
GAAACAAACG GAGGGAAAAA GGAGTTTTCT CTGTTTTGCG ACGAGCGCGG GTATTTGAGT 
CTGGTGGAGT TTATTTTGCA GAATGGAGCA AAGAAAGGCG ACAGGACAGG CACAGGAGTG 
ATCTCTGTGT TCGGGACACA AGCCAGATAC AGTCTCAGAG ATCAGTTTCC GTTGCTGACG 
ACCAAAAGAG TTTTCTGGAA AGGCATATTG GAGGAGCTGC TGTGGTTCAT CAAGGGTTCA 
ACAAACGCCA AAGATCTGTC GGAGAAGGGT GTGAGGATTT GGGATGCTAA CGGCTCCAGA 
GAGTTTCTGG ATAAGAACGG ATTCACCGAT CGGGAGGAAG GAGATCTGGG TCCAGTTTAT 
GGATTTCAGT GGAGGCATTT TGGAGCCGAA TACAAAGATA TGCATACTGA TTATTCTGGA 
GAAGGAGTCG ATCAGTTACA GAAAGTTATT GACACCATCA AGTCAAACCC AGAGGACCGA 
AGGATCATCA TGTGTGCCTG GAATCCTAAA GATCTCCCTC TGATGGCGCT GCCGCCCTGT 
CATGCGTTGT GTCAGTTTTA CGTGTCGAAC GGTGAGTTGT CATGTCAGCT GTACCAGCGA 
TCGGGAGACA TCGGTCTCGG TGTCCCATTC AACATCGCCA GCTACGCCCT GCTGACCTAC 
ATGATCGCCC ACATCACTGG ACTCAAGCCT GGGGATTTCG TTCATACATT AGGTGACGCC 
CACATCTACA CCAATCACAT CGAGCCTTTA AAAGAGCAGA TTCAGCGAGA GCCGCGTCCG 
TTCCCCAAAC TCCGGATTAA ACGCAAAGTT GAACAAATTA ATGATTTCTG TGCTGAAGAT 
TTTGAGATCT ATGATTATAA CCCACATCCT ACAATCAAAA TGCAAATGGC TGTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_571835.2
CDS	165..1121
Translation	MPDTAVEVTNGHCVNGESKKETNGGKKEFSLFCDERGYLSLVEFILQNGAKKGDRTGTGVISVFGTQARYSLRDQFPLLTTKRVFWKGILEELLWFIKGSTNAKDLSEKGVRIWDANGSREFLDKNGFTDREEGDLGPVYGFQWRHFGAEYKDMHTDYSGEGVDQLQKVIDTIKSNPEDRRIIMCAWNPKDLPLMALPPCHALCQFYVSNGELSCQLYQRSGDIGLGVPFNIASYALLTYMIAHITGLKPGDFVHTLGDAHIYTNHIEPLKEQIQREPRPFPKLRIKRKVEQINDFCAEDFEIYDYNPHPTIKMQMAV

Target ORF information:

RefSeq Version	NM_131760.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio thymidylate synthetase (tyms), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_131760.2

ORF Insert Sequence:

ATGCCCGACA CGGCAGTGGA AGTGACAAAT GGACATTGCG TTAACGGAGA AAGCAAGAAA 
GAAACAAACG GAGGGAAAAA GGAGTTTTCT CTGTTTTGCG ACGAGCGCGG GTATTTGAGT 
CTGGTGGAGT TTATTTTGCA GAATGGAGCA AAGAAAGGCG ACAGGACAGG CACAGGAGTG 
ATCTCTGTGT TCGGGACACA AGCCAGATAC AGTCTCAGAG ATCAGTTTCC GTTGCTGACG 
ACCAAAAGAG TTTTCTGGAA AGGCATATTG GAGGAGCTGC TGTGGTTCAT CAAGGGTTCA 
ACAAACGCCA AAGATCTGTC GGAGAAGGGT GTGAGGATTT GGGATGCTAA CGGCTCCAGA 
GAGTTTCTGG ATAAGAACGG ATTCACCGAT CGGGAGGAAG GAGATCTGGG TCCAGTTTAT 
GGATTTCAGT GGAGGCATTT TGGAGCCGAA TACAAAGATA TGCATACTGA TTATTCTGGA 
GAAGGAGTCG ATCAGTTACA GAAAGTTATT GACACCATCA AGTCAAACCC AGAGGACCGA 
AGGATCATCA TGTGTGCCTG GAATCCTAAA GATCTCCCTC TGATGGCGCT GCCGCCCTGT 
CATGCGTTGT GTCAGTTTTA CGTGTCGAAC GGTGAGTTGT CATGTCAGCT GTACCAGCGA 
TCGGGAGACA TCGGTCTCGG TGTCCCATTC AACATCGCCA GCTACGCCCT GCTGACCTAC 
ATGATCGCCC ACATCACTGG ACTCAAGCCT GGGGATTTCG TTCATACATT AGGTGACGCC 
CACATCTACA CCAATCACAT CGAGCCTTTA AAAGAGCAGA TTCAGCGAGA GCCGCGTCCG 
TTCCCCAAAC TCCGGATTAA ACGCAAAGTT GAACAAATTA ATGATTTCTG TGCTGAAGAT 
TTTGAGATCT ATGATTATAA CCCACATCCT ACAATCAAAA TGCAAATGGC TGTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Dynamic phosphorylation of Histone Deacetylase 1 by Aurora kinases during mitosis regulates zebrafish embryos development.
Scientific reports630213(2016 Jul)
Loponte S,Segr? CV,Senese S,Miccolo C,Santaguida S,Deflorian G,Citro S,Mattoscio D,Pisati F,Moser MA,Visintin R,Seiser C,Chiocca S

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase (cad) regulates Notch signaling and vascular development in zebrafish.
Developmental dynamics : an official publication of the American Association of Anatomists244(1)1-9(2015 Jan)
Coxam B,Neyt C,Grassini DR,Le Guen L,Smith KA,Schulte-Merker S,Hogan BM

Zebrafish midbrain slow-amplifying progenitors exhibit high levels of transcripts for nucleotide and ribosome biogenesis.
Development (Cambridge, England)140(24)4860-9(2013 Dec)
Recher G,Jouralet J,Brombin A,Heuz? A,Mugniery E,Hermel JM,Desnoulez S,Savy T,Herbomel P,Bourrat F,Peyri?ras N,Jamen F,Joly JS

Regulation of E2F1 by the von Hippel-Lindau tumour suppressor protein predicts survival in renal cell cancer patients.
The Journal of pathology231(1)117-29(2013 Sep)
Mans DA,Vermaat JS,Weijts BG,van Rooijen E,van Reeuwijk J,Boldt K,Daenen LG,van der Groep P,Rowland BD,Jans JJ,Roepman R,Voest EE,van Diest PJ,Verhaar MC,de Bruin A,Giles RH

見積依頼

Eメール

japanmarket@genscript.com

電話

03-6811-6572

FAX

03-6811-6573
050-3174-7589(受注専用)

オンラインフォーム

オンライン注文

tyms cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

会社情報

サポート

会社情報