dennd5a cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	dennd5a
Entrez Gene ID	799847
Full Name	DENN/MADD domain containing 5A
Synonyms	rab6ip1
General protein information	Preferred Names DENN/MADD domain containing 5A Names DENN domain-containing protein 5A rab6 interacting protein 1
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009303571.3	XP_009301846.1	DENN domain-containing protein 5A isoform X1
XM_009303572.3	XP_009301847.1	DENN domain-containing protein 5A isoform X2
XM_005166611.3	XP_005166668.1	DENN domain-containing protein 5A isoform X4
XM_001340126.8	XP_001340162.4	DENN domain-containing protein 5A isoform X3
XM_009303572.2	XP_009301847.1	DENN domain-containing protein 5A isoform X2
XM_009303571.2	XP_009301846.1	DENN domain-containing protein 5A isoform X1
XM_001340126.7	XP_001340162.4	DENN domain-containing protein 5A isoform X3
XM_005166611.4	XP_005166668.1	DENN domain-containing protein 5A isoform X4

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	DENND5A	XP_508914.2
Bos taurus (cattle)	DENND5A	XP_002693152.1
Danio rerio (zebrafish)	dennd5a	XP_001340162.4
Rattus norvegicus (Norway rat)	Dennd5a	NP_001101016.2
Caenorhabditis elegans (roundworm)	C52E12.4	NP_495437.2
Xenopus tropicalis (tropical clawed frog)	dennd5a	NP_001121468.1
Homo sapiens (human)	DENND5A	NP_056028.2
	DENND5A	XP_002799687.1
Gallus gallus (chicken)	DENND5A	XP_004941436.1
Canis lupus familiaris (dog)	DENND5A	XP_534048.3
Mus musculus (house mouse)	Dennd5a	NP_067469.1

Gene Ontology

The following dennd5a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the dennd5a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa13598	XM_001340126.8 Latest version!	Danio rerio DENN/MADD domain containing 5A (dennd5a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
ODa13599	XM_005166611.4 Latest version!	Danio rerio DENN/MADD domain containing 5A (dennd5a), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
ODa47407	XM_009303571.3 Latest version!	Danio rerio DENN/MADD domain containing 5A (dennd5a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
ODa47408	XM_009303572.3 Latest version!	Danio rerio DENN/MADD domain containing 5A (dennd5a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
View Old Accession Versions >>
ODa47408	XM_009303572.2	Danio rerio DENN/MADD domain containing 5A (dennd5a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
ODa47407	XM_009303571.2	Danio rerio DENN/MADD domain containing 5A (dennd5a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
ODa13598	XM_001340126.7	Danio rerio DENN/MADD domain containing 5A (dennd5a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
ODa13599	XM_005166611.3	Danio rerio DENN/MADD domain containing 5A (dennd5a), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

dennd5a ( XM_001340126.8 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_001340162.4 Danio rerio DENN/MADD domain containing 5A (dennd5a), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa13598
Clone ID Related Accession (Same CDS sequence)	XM_001340126.8 , XM_001340126.7
Accession Version	XM_001340126.8 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3867bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	DENN domain-containing protein 5A isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_001340126.7. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCACCG GATTCAGCTC CAGCTCCTGC CGCTTCGCGG ATTATTTCGT GATCTGCGGT 
CTGGACACCG AGAGCGGCTT AGAACCAGAC GAGCTGTCCG CTTTATGCCA GTATATTCAG 
GCTACTAAAT TCACAGATGG CGCCAGAGGA AAGTTGGCGA GTGCAAATGA AGGTGAGAAT 
TTTGAGCAGA GTCCACTGCG GAGAACGTTT AAATCGAAGG TTCTAGCGCA TTATCCTGAC 
AATGTGGAGT GGAACCCTTT TGACCAGGAT GCAGTGGGAA TGCTCTGTAT GCCAAAAGGC 
CTGTCCTTCC GCACTCAAAC AGACACCCGT GAGCCCCAGT TCCACTCCTT CATTATCACC 
AGAGAGGACG GCTCCCGGAC GTACGGCTTT GCCCTCACTT TCTTTGAGGA GGTGACCAGC 
AAACAGATCT GCAGTGCCAT GCAGACACTT TATCACATGC ACAATGCGGA ACAGTATGAT 
ATCCTGCACA CACCACCCAC GCCTCGCAGT CCAGAAGATC CCCGTCCCAC CACGCAACAA 
CTGCACGCCT CCCCTGCTCT GTCCAGGCTG CAGCGCTTCA ACTCATACGA CATCAGCCGG 
GACACGCTGT ACGTGTCCAA ATGCATCTGC CTGATCACCC CCATGGCCTT CGCCCACGCC 
TGCAGGAAGG TTCTGGAGCA GCTGCATCAG GCCGTCACCT CACCTCAGCC CCCGCCGCTG 
CCGCTGGAGA GCTATGTCTA CAATGTCCTC TATGAGGTCC CACTGCCACC AGCCGGACGC 
TCCTTGAAGT TTTCAGGCGT CTACAGGCCA GTGATCTGTC AGAGACCCAG CACAGCCGAG 
CTGCCCCTCT TTGACTTCCC CCTTCAGGAG GTGTTTGAGC TGCTAGGGCT GGAGAACATC 
TTGCAGCTCT TCACCTGTGC GCTGCTGGAG ATCCAGATCC TTCTGTACTC ACAACATTAC 
CAGCGGCTGA TGACGGTGGC GGAGAGCATC ACAGCCTTAA TGTTCCCCTT TCAGTGGCAG 
CATGTGTACG TGCCCATCCT GCCCGCTTCC CTGCTCCACT TCCTGGACGC TCCTGTGCCG 
TACCTCATGG GTCTGCACTC TAACGGCCAG GATGACCGCA CCAAACTGGA GCTTCCCCAA 
GAGGCCAATC TCTGCTTTGT CGATATTGAC AACCACTTCA TCGAGCTACC CGAAGACCTG 
CCTCAGTTCC CCAACAAGCT GGAGTTTATC CAGGAGATCT CTGAGGTTCT GCTGGCGTTT 
GGCTTGTCTC CAGAAGCGAA CCCACAATGC AGCGAGGGCC AGAGCAAGAT CAGGGGCTTC 
CGGTGTGGCG ACATGGCCTC CGACAAGCGC AACGGGAACC TGGCCGGCTC GCCGCTCAAT 
TCGTTCCTGC TGAGAGAAAA CCAAACCATC GCTCGCCTGC AAGCGCTGGT CAAGAGGACT 
GGAGTCAGTC TGGAGAAGTT GGACGTGAAG GACGACGCAA GCACTAATAA GGATACGAAA 
GTGCAGTGTG AAGAAGAAGA GATGAAAATG CACCAGCTGA ACATTCGGGT GCGAGAGGTC 
TTCGCCAACC GTTTCTCTCA GATGTTCTCG GATTATGAGG TGTTTGTTAT TCAGCCCAGC 
CAAGACAAAG AGTCCTGGTT CAGCAACCGA GACCAGATGC AGAACTTTGA CAAGGCCTCC 
TTCCTGTCAG ATCAGCCCGA ACCGTACCTG CCGTTCCTCT CCCGCTTTCT GGAGACGCAG 
ATGTTTGCCT CTTTTATCGA CAGTAAGATT CTGTGCCACG ATGACGAGGA CAAAGAGCAC 
ACACTGCGAG TCTTTGATTC CCGTGTTGAC AAGATCCGCA TGATAAATGT CAGGACACCC 
ACCCTGCGGA CCTCCATGTA CCAGAAATGC ACCAATATCG ATGAAGCAGA AAAAGCCATA 
GAGGTGAGAC TATGTAAGAT CGACCACACG GCTCTGCATC CCCATCTGCT GGACATGAAG 
ATCGGTCAGG GCCGCTATGA GCCAGGCTTT TTCCCTCGCT TACAGTCTGA TGTGCTCTCC 
ACTGGACCCA CCAGCAACAA GTGGGCGAAG CGCAGTGCAC CGGCCCAATG GCGGAGGAAA 
GACCGACAGA AACAGCACGC CGAACATCTC TATCTGGACA ATGACCAGAG AGAGAAATAC 
ATCCAGGAAG CCAGAAACCT TGGCACCACA CTCCGTCAGC CAAAACTCTC CAACCTGTCG 
CCTTCGGTCA TTGCACAGAC CAACTGGAAG TTTGTTGAGG GACTGTTGAA GGAGTGCAGA 
AATAAGACAA AGCGCATGCT GGTGGAGAAG ATGGGACGGG AGGCAGTGGA GCTGGGCCAC 
GGTGAGGTGA GCATCACCGG TGTGGAGGAA AACACACTCA TCGCCAGCCT GTGTGACCTG 
CTGGAGCGAA TCTGGAGCCA CGGCCTACAG GTCAAACAGG GAAAATCCGC CCTGTGGTCG 
CACCTTTTAC ATTATCAAGA GAGTAAAGAG AAGAGAGACG CTACTCCAGC AGGCCTCGGC 
CCTCCTGGTA TCATTCATGA CACAGAGAGA CGGAAGTCTG ATGCTAGCTG TGCGATGCCG 
CCTCTCAAAG TCTCCTTGAT TCAGGACATG AGGCACATCC AGAACATCAG CGAGATCAAG 
ACGGACGTTG GCAAGGCCAG GGCTTGGGTG CGTCTCTCCA TGGAGAAGAA ACTGCTGTCC 
AGACACCTGA AGCAACTGCT GTCTGACCAC GAGCTCACCA AGAAACTCTA TAAGCGATAT 
GCTTTTCTGC GCTGTGATGA CGAGAAGGAG CAGTTCCTCT ACCATCTCCT CTCTTTCAAC 
GCTGTGGACT ACTTCTGCTT CACTAATGTC TTCACCACAA TTATGATTCC ATACCATGTG 
GTCATCATTC CTAGTAAGAA ACTCGGTGGC TCTATGTTCA CAGCCAACCC CTGGGTGTGT 
GTCTCAGGGG AACTAGCCGA GACTGGGGTC CTGCAGGTCC CTAAAAACAC ACTGGAGATT 
ACATTTGAGT GCCAGAATCT GGGGAAGCTG ACCACTGTAC AGATGGGCCA TGATAACTCA 
GGGCTGTATG CTAAATGGCT AGTGGAGTGC GTAATGGTCC GCAATGGGAT TACAGGACAC 
ACCTATAAGT TTCCATGTGG CCGTTGGCTG GGTAAAGGAG TAGATGATGG CAGCTTGGAG 
AGGATCCTGG TGGGCGAATT GGTGACTAAC ATGGAGAGCG ATGAAAGAAT GTGCCGGACT 
CCTCCGATGC AGCAATCCCC CGGGATGATG CGAAGATTTG TCACCATTTC GCCAAACAGC 
AAACCAAAGT TAAACACGGG CCAGATCCAG GAAAGTGTGG GAGAGGCGAT TAACGGGATC 
GTCAAACACT TCCATAAGCC GGAGAAAGAG AGAGGAAGCC TCACGTTGCT TCTGTGTGGG 
GAGTATGGTT TGGTTTGGGC TCTGGAGCAG GTGTTTCAGC ATGGCTTCAA GTCACCGCGA 
CTGTTCAAGA ACGTCTTCAT TTGGGACTTT CTAGAAAAGG CGCAGGTTTA CTTTGAGAGT 
GCGGAGCGGA GTCAGATGAC CCAGGATGAG AACTGGCAGA TGAGGGTCCG TCATTTCTGC 
CATTTCATGC GCGCCATCAA CAGCACACCC AGAAACATCG GCAAAGATGG GAAGTTTCAG 
ATGCTTGTGT GTCTTGGGGC CAGAGATCAC CTGCTCCACC ATTGGATCGC GCTTCTTGCC 
GAGTGTCCAA TCACAGCACA GATGTACGAG GACGTGGCGC TCTTAAAGGA CCATTCATTG 
GTGAACTCTC TGATTCGAGT ACTACAGACT CTGCAGGAGT TCAATATCAC TCTGGAAGCT 
TCTCTCGTCA AAGGGATTGG GATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001340162.4
CDS	210..4076
Translation	MSTGFSSSSCRFADYFVICGLDTESGLEPDELSALCQYIQATKFTDGARGKLASANEGENFEQSPLRRTFKSKVLAHYPDNVEWNPFDQDAVGMLCMPKGLSFRTQTDTREPQFHSFIITREDGSRTYGFALTFFEEVTSKQICSAMQTLYHMHNAEQYDILHTPPTPRSPEDPRPTTQQLHASPALSRLQRFNSYDISRDTLYVSKCICLITPMAFAHACRKVLEQLHQAVTSPQPPPLPLESYVYNVLYEVPLPPAGRSLKFSGVYRPVICQRPSTAELPLFDFPLQEVFELLGLENILQLFTCALLEIQILLYSQHYQRLMTVAESITALMFPFQWQHVYVPILPASLLHFLDAPVPYLMGLHSNGQDDRTKLELPQEANLCFVDIDNHFIELPEDLPQFPNKLEFIQEISEVLLAFGLSPEANPQCSEGQSKIRGFRCGDMASDKRNGNLAGSPLNSFLLRENQTIARLQALVKRTGVSLEKLDVKDDASTNKDTKVQCEEEEMKMHQLNIRVREVFANRFSQMFSDYEVFVIQPSQDKESWFSNRDQMQNFDKASFLSDQPEPYLPFLSRFLETQMFASFIDSKILCHDDEDKEHTLRVFDSRVDKIRMINVRTPTLRTSMYQKCTNIDEAEKAIEVRLCKIDHTALHPHLLDMKIGQGRYEPGFFPRLQSDVLSTGPTSNKWAKRSAPAQWRRKDRQKQHAEHLYLDNDQREKYIQEARNLGTTLRQPKLSNLSPSVIAQTNWKFVEGLLKECRNKTKRMLVEKMGREAVELGHGEVSITGVEENTLIASLCDLLERIWSHGLQVKQGKSALWSHLLHYQESKEKRDATPAGLGPPGIIHDTERRKSDASCAMPPLKVSLIQDMRHIQNISEIKTDVGKARAWVRLSMEKKLLSRHLKQLLSDHELTKKLYKRYAFLRCDDEKEQFLYHLLSFNAVDYFCFTNVFTTIMIPYHVVIIPSKKLGGSMFTANPWVCVSGELAETGVLQVPKNTLEITFECQNLGKLTTVQMGHDNSGLYAKWLVECVMVRNGITGHTYKFPCGRWLGKGVDDGSLERILVGELVTNMESDERMCRTPPMQQSPGMMRRFVTISPNSKPKLNTGQIQESVGEAINGIVKHFHKPEKERGSLTLLLCGEYGLVWALEQVFQHGFKSPRLFKNVFIWDFLEKAQVYFESAERSQMTQDENWQMRVRHFCHFMRAINSTPRNIGKDGKFQMLVCLGARDHLLHHWIALLAECPITAQMYEDVALLKDHSLVNSLIRVLQTLQEFNITLEASLVKGIGI

Target ORF information:

RefSeq Version	XM_001340126.8
Organism	Danio rerio(zebrafish)
Definition	Danio rerio DENN/MADD domain containing 5A (dennd5a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001340126.8

ORF Insert Sequence:

ATGAGCACCG GATTCAGCTC CAGCTCCTGC CGCTTCGCGG ATTATTTCGT GATCTGCGGT 
CTGGACACCG AGAGCGGCTT AGAACCAGAC GAGCTGTCCG CTTTATGCCA GTATATTCAG 
GCTACTAAAT TCACAGATGG CGCCAGAGGA AAGTTGGCGA GTGCAAATGA AGGTGAGAAT 
TTTGAGCAGA GTCCACTGCG GAGAACGTTT AAATCGAAGG TTCTAGCGCA TTATCCTGAC 
AATGTGGAGT GGAACCCTTT TGACCAGGAT GCAGTGGGAA TGCTCTGTAT GCCAAAAGGC 
CTGTCCTTCC GCACTCAAAC AGACACCCGT GAGCCCCAGT TCCACTCCTT CATTATCACC 
AGAGAGGACG GCTCCCGGAC GTACGGCTTT GCCCTCACTT TCTTTGAGGA GGTGACCAGC 
AAACAGATCT GCAGTGCCAT GCAGACACTT TATCACATGC ACAATGCGGA ACAGTATGAT 
ATCCTGCACA CACCACCCAC GCCTCGCAGT CCAGAAGATC CCCGTCCCAC CACGCAACAA 
CTGCACGCCT CCCCTGCTCT GTCCAGGCTG CAGCGCTTCA ACTCATACGA CATCAGCCGG 
GACACGCTGT ACGTGTCCAA ATGCATCTGC CTGATCACCC CCATGGCCTT CGCCCACGCC 
TGCAGGAAGG TTCTGGAGCA GCTGCATCAG GCCGTCACCT CACCTCAGCC CCCGCCGCTG 
CCGCTGGAGA GCTATGTCTA CAATGTCCTC TATGAGGTCC CACTGCCACC AGCCGGACGC 
TCCTTGAAGT TTTCAGGCGT CTACAGGCCA GTGATCTGTC AGAGACCCAG CACAGCCGAG 
CTGCCCCTCT TTGACTTCCC CCTTCAGGAG GTGTTTGAGC TGCTAGGGCT GGAGAACATC 
TTGCAGCTCT TCACCTGTGC GCTGCTGGAG ATCCAGATCC TTCTGTACTC ACAACATTAC 
CAGCGGCTGA TGACGGTGGC GGAGAGCATC ACAGCCTTAA TGTTCCCCTT TCAGTGGCAG 
CATGTGTACG TGCCCATCCT GCCCGCTTCC CTGCTCCACT TCCTGGACGC TCCTGTGCCG 
TACCTCATGG GTCTGCACTC TAACGGCCAG GATGACCGCA CCAAACTGGA GCTTCCCCAA 
GAGGCCAATC TCTGCTTTGT CGATATTGAC AACCACTTCA TCGAGCTACC CGAAGACCTG 
CCTCAGTTCC CCAACAAGCT GGAGTTTATC CAGGAGATCT CTGAGGTTCT GCTGGCGTTT 
GGCTTGTCTC CAGAAGCGAA CCCACAATGC AGCGAGGGCC AGAGCAAGAT CAGGGGCTTC 
CGGTGTGGCG ACATGGCCTC CGACAAGCGC AACGGGAACC TGGCCGGCTC GCCGCTCAAT 
TCGTTCCTGC TGAGAGAAAA CCAAACCATC GCTCGCCTGC AAGCGCTGGT CAAGAGGACT 
GGAGTCAGTC TGGAGAAGTT GGACGTGAAG GACGACGCAA GCACTAATAA GGATACGAAA 
GTGCAGTGTG AAGAAGAAGA GATGAAAATG CACCAGCTGA ACATTCGGGT GCGAGAGGTC 
TTCGCCAACC GTTTCTCTCA GATGTTCTCG GATTATGAGG TGTTTGTTAT TCAGCCCAGC 
CAAGACAAAG AGTCCTGGTT CAGCAACCGA GACCAGATGC AGAACTTTGA CAAGGCCTCC 
TTCCTGTCAG ATCAGCCCGA ACCGTACCTG CCGTTCCTCT CCCGCTTTCT GGAGACGCAG 
ATGTTTGCCT CTTTTATCGA CAGTAAGATT CTGTGCCACG ATGACGAGGA CAAAGAGCAC 
ACACTGCGAG TCTTTGATTC CCGTGTTGAC AAGATCCGCA TGATAAATGT CAGGACACCC 
ACCCTGCGGA CCTCCATGTA CCAGAAATGC ACCAATATCG ATGAAGCAGA AAAAGCCATA 
GAGGTGAGAC TATGTAAGAT CGACCACACG GCTCTGCATC CCCATCTGCT GGACATGAAG 
ATCGGTCAGG GCCGCTATGA GCCAGGCTTT TTCCCTCGCT TACAGTCTGA TGTGCTCTCC 
ACTGGACCCA CCAGCAACAA GTGGGCGAAG CGCAGTGCAC CGGCCCAATG GCGGAGGAAA 
GACCGACAGA AACAGCACGC CGAACATCTC TATCTGGACA ATGACCAGAG AGAGAAATAC 
ATCCAGGAAG CCAGAAACCT TGGCACCACA CTCCGTCAGC CAAAACTCTC CAACCTGTCG 
CCTTCGGTCA TTGCACAGAC CAACTGGAAG TTTGTTGAGG GACTGTTGAA GGAGTGCAGA 
AATAAGACAA AGCGCATGCT GGTGGAGAAG ATGGGACGGG AGGCAGTGGA GCTGGGCCAC 
GGTGAGGTGA GCATCACCGG TGTGGAGGAA AACACACTCA TCGCCAGCCT GTGTGACCTG 
CTGGAGCGAA TCTGGAGCCA CGGCCTACAG GTCAAACAGG GAAAATCCGC CCTGTGGTCG 
CACCTTTTAC ATTATCAAGA GAGTAAAGAG AAGAGAGACG CTACTCCAGC AGGCCTCGGC 
CCTCCTGGTA TCATTCATGA CACAGAGAGA CGGAAGTCTG ATGCTAGCTG TGCGATGCCG 
CCTCTCAAAG TCTCCTTGAT TCAGGACATG AGGCACATCC AGAACATCAG CGAGATCAAG 
ACGGACGTTG GCAAGGCCAG GGCTTGGGTG CGTCTCTCCA TGGAGAAGAA ACTGCTGTCC 
AGACACCTGA AGCAACTGCT GTCTGACCAC GAGCTCACCA AGAAACTCTA TAAGCGATAT 
GCTTTTCTGC GCTGTGATGA CGAGAAGGAG CAGTTCCTCT ACCATCTCCT CTCTTTCAAC 
GCTGTGGACT ACTTCTGCTT CACTAATGTC TTCACCACAA TTATGATTCC ATACCATGTG 
GTCATCATTC CTAGTAAGAA ACTCGGTGGC TCTATGTTCA CAGCCAACCC CTGGGTGTGT 
GTCTCAGGGG AACTAGCCGA GACTGGGGTC CTGCAGGTCC CTAAAAACAC ACTGGAGATT 
ACATTTGAGT GCCAGAATCT GGGGAAGCTG ACCACTGTAC AGATGGGCCA TGATAACTCA 
GGGCTGTATG CTAAATGGCT AGTGGAGTGC GTAATGGTCC GCAATGGGAT TACAGGACAC 
ACCTATAAGT TTCCATGTGG CCGTTGGCTG GGTAAAGGAG TAGATGATGG CAGCTTGGAG 
AGGATCCTGG TGGGCGAATT GGTGACTAAC ATGGAGAGCG ATGAAAGAAT GTGCCGGACT 
CCTCCGATGC AGCAATCCCC CGGGATGATG CGAAGATTTG TCACCATTTC GCCAAACAGC 
AAACCAAAGT TAAACACGGG CCAGATCCAG GAAAGTGTGG GAGAGGCGAT TAACGGGATC 
GTCAAACACT TCCATAAGCC GGAGAAAGAG AGAGGAAGCC TCACGTTGCT TCTGTGTGGG 
GAGTATGGTT TGGTTTGGGC TCTGGAGCAG GTGTTTCAGC ATGGCTTCAA GTCACCGCGA 
CTGTTCAAGA ACGTCTTCAT TTGGGACTTT CTAGAAAAGG CGCAGGTTTA CTTTGAGAGT 
GCGGAGCGGA GTCAGATGAC CCAGGATGAG AACTGGCAGA TGAGGGTCCG TCATTTCTGC 
CATTTCATGC GCGCCATCAA CAGCACACCC AGAAACATCG GCAAAGATGG GAAGTTTCAG 
ATGCTTGTGT GTCTTGGGGC CAGAGATCAC CTGCTCCACC ATTGGATCGC GCTTCTTGCC 
GAGTGTCCAA TCACAGCACA GATGTACGAG GACGTGGCGC TCTTAAAGGA CCATTCATTG 
GTGAACTCTC TGATTCGAGT ACTACAGACT CTGCAGGAGT TCAATATCAC TCTGGAAGCT 
TCTCTCGTCA AAGGGATTGG GATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dennd5a ( XM_001340126.7 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_001340162.4 Danio rerio DENN/MADD domain containing 5A (dennd5a), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa13598
Clone ID Related Accession (Same CDS sequence)	XM_001340126.8 , XM_001340126.7
Accession Version	XM_001340126.7	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3867bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	DENN domain-containing protein 5A isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_001340126.6. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCACCG GATTCAGCTC CAGCTCCTGC CGCTTCGCGG ATTATTTCGT GATCTGCGGT 
CTGGACACCG AGAGCGGCTT AGAACCAGAC GAGCTGTCCG CTTTATGCCA GTATATTCAG 
GCTACTAAAT TCACAGATGG CGCCAGAGGA AAGTTGGCGA GTGCAAATGA AGGTGAGAAT 
TTTGAGCAGA GTCCACTGCG GAGAACGTTT AAATCGAAGG TTCTAGCGCA TTATCCTGAC 
AATGTGGAGT GGAACCCTTT TGACCAGGAT GCAGTGGGAA TGCTCTGTAT GCCAAAAGGC 
CTGTCCTTCC GCACTCAAAC AGACACCCGT GAGCCCCAGT TCCACTCCTT CATTATCACC 
AGAGAGGACG GCTCCCGGAC GTACGGCTTT GCCCTCACTT TCTTTGAGGA GGTGACCAGC 
AAACAGATCT GCAGTGCCAT GCAGACACTT TATCACATGC ACAATGCGGA ACAGTATGAT 
ATCCTGCACA CACCACCCAC GCCTCGCAGT CCAGAAGATC CCCGTCCCAC CACGCAACAA 
CTGCACGCCT CCCCTGCTCT GTCCAGGCTG CAGCGCTTCA ACTCATACGA CATCAGCCGG 
GACACGCTGT ACGTGTCCAA ATGCATCTGC CTGATCACCC CCATGGCCTT CGCCCACGCC 
TGCAGGAAGG TTCTGGAGCA GCTGCATCAG GCCGTCACCT CACCTCAGCC CCCGCCGCTG 
CCGCTGGAGA GCTATGTCTA CAATGTCCTC TATGAGGTCC CACTGCCACC AGCCGGACGC 
TCCTTGAAGT TTTCAGGCGT CTACAGGCCA GTGATCTGTC AGAGACCCAG CACAGCCGAG 
CTGCCCCTCT TTGACTTCCC CCTTCAGGAG GTGTTTGAGC TGCTAGGGCT GGAGAACATC 
TTGCAGCTCT TCACCTGTGC GCTGCTGGAG ATCCAGATCC TTCTGTACTC ACAACATTAC 
CAGCGGCTGA TGACGGTGGC GGAGAGCATC ACAGCCTTAA TGTTCCCCTT TCAGTGGCAG 
CATGTGTACG TGCCCATCCT GCCCGCTTCC CTGCTCCACT TCCTGGACGC TCCTGTGCCG 
TACCTCATGG GTCTGCACTC TAACGGCCAG GATGACCGCA CCAAACTGGA GCTTCCCCAA 
GAGGCCAATC TCTGCTTTGT CGATATTGAC AACCACTTCA TCGAGCTACC CGAAGACCTG 
CCTCAGTTCC CCAACAAGCT GGAGTTTATC CAGGAGATCT CTGAGGTTCT GCTGGCGTTT 
GGCTTGTCTC CAGAAGCGAA CCCACAATGC AGCGAGGGCC AGAGCAAGAT CAGGGGCTTC 
CGGTGTGGCG ACATGGCCTC CGACAAGCGC AACGGGAACC TGGCCGGCTC GCCGCTCAAT 
TCGTTCCTGC TGAGAGAAAA CCAAACCATC GCTCGCCTGC AAGCGCTGGT CAAGAGGACT 
GGAGTCAGTC TGGAGAAGTT GGACGTGAAG GACGACGCAA GCACTAATAA GGATACGAAA 
GTGCAGTGTG AAGAAGAAGA GATGAAAATG CACCAGCTGA ACATTCGGGT GCGAGAGGTC 
TTCGCCAACC GTTTCTCTCA GATGTTCTCG GATTATGAGG TGTTTGTTAT TCAGCCCAGC 
CAAGACAAAG AGTCCTGGTT CAGCAACCGA GACCAGATGC AGAACTTTGA CAAGGCCTCC 
TTCCTGTCAG ATCAGCCCGA ACCGTACCTG CCGTTCCTCT CCCGCTTTCT GGAGACGCAG 
ATGTTTGCCT CTTTTATCGA CAGTAAGATT CTGTGCCACG ATGACGAGGA CAAAGAGCAC 
ACACTGCGAG TCTTTGATTC CCGTGTTGAC AAGATCCGCA TGATAAATGT CAGGACACCC 
ACCCTGCGGA CCTCCATGTA CCAGAAATGC ACCAATATCG ATGAAGCAGA AAAAGCCATA 
GAGGTGAGAC TATGTAAGAT CGACCACACG GCTCTGCATC CCCATCTGCT GGACATGAAG 
ATCGGTCAGG GCCGCTATGA GCCAGGCTTT TTCCCTCGCT TACAGTCTGA TGTGCTCTCC 
ACTGGACCCA CCAGCAACAA GTGGGCGAAG CGCAGTGCAC CGGCCCAATG GCGGAGGAAA 
GACCGACAGA AACAGCACGC CGAACATCTC TATCTGGACA ATGACCAGAG AGAGAAATAC 
ATCCAGGAAG CCAGAAACCT TGGCACCACA CTCCGTCAGC CAAAACTCTC CAACCTGTCG 
CCTTCGGTCA TTGCACAGAC CAACTGGAAG TTTGTTGAGG GACTGTTGAA GGAGTGCAGA 
AATAAGACAA AGCGCATGCT GGTGGAGAAG ATGGGACGGG AGGCAGTGGA GCTGGGCCAC 
GGTGAGGTGA GCATCACCGG TGTGGAGGAA AACACACTCA TCGCCAGCCT GTGTGACCTG 
CTGGAGCGAA TCTGGAGCCA CGGCCTACAG GTCAAACAGG GAAAATCCGC CCTGTGGTCG 
CACCTTTTAC ATTATCAAGA GAGTAAAGAG AAGAGAGACG CTACTCCAGC AGGCCTCGGC 
CCTCCTGGTA TCATTCATGA CACAGAGAGA CGGAAGTCTG ATGCTAGCTG TGCGATGCCG 
CCTCTCAAAG TCTCCTTGAT TCAGGACATG AGGCACATCC AGAACATCAG CGAGATCAAG 
ACGGACGTTG GCAAGGCCAG GGCTTGGGTG CGTCTCTCCA TGGAGAAGAA ACTGCTGTCC 
AGACACCTGA AGCAACTGCT GTCTGACCAC GAGCTCACCA AGAAACTCTA TAAGCGATAT 
GCTTTTCTGC GCTGTGATGA CGAGAAGGAG CAGTTCCTCT ACCATCTCCT CTCTTTCAAC 
GCTGTGGACT ACTTCTGCTT CACTAATGTC TTCACCACAA TTATGATTCC ATACCATGTG 
GTCATCATTC CTAGTAAGAA ACTCGGTGGC TCTATGTTCA CAGCCAACCC CTGGGTGTGT 
GTCTCAGGGG AACTAGCCGA GACTGGGGTC CTGCAGGTCC CTAAAAACAC ACTGGAGATT 
ACATTTGAGT GCCAGAATCT GGGGAAGCTG ACCACTGTAC AGATGGGCCA TGATAACTCA 
GGGCTGTATG CTAAATGGCT AGTGGAGTGC GTAATGGTCC GCAATGGGAT TACAGGACAC 
ACCTATAAGT TTCCATGTGG CCGTTGGCTG GGTAAAGGAG TAGATGATGG CAGCTTGGAG 
AGGATCCTGG TGGGCGAATT GGTGACTAAC ATGGAGAGCG ATGAAAGAAT GTGCCGGACT 
CCTCCGATGC AGCAATCCCC CGGGATGATG CGAAGATTTG TCACCATTTC GCCAAACAGC 
AAACCAAAGT TAAACACGGG CCAGATCCAG GAAAGTGTGG GAGAGGCGAT TAACGGGATC 
GTCAAACACT TCCATAAGCC GGAGAAAGAG AGAGGAAGCC TCACGTTGCT TCTGTGTGGG 
GAGTATGGTT TGGTTTGGGC TCTGGAGCAG GTGTTTCAGC ATGGCTTCAA GTCACCGCGA 
CTGTTCAAGA ACGTCTTCAT TTGGGACTTT CTAGAAAAGG CGCAGGTTTA CTTTGAGAGT 
GCGGAGCGGA GTCAGATGAC CCAGGATGAG AACTGGCAGA TGAGGGTCCG TCATTTCTGC 
CATTTCATGC GCGCCATCAA CAGCACACCC AGAAACATCG GCAAAGATGG GAAGTTTCAG 
ATGCTTGTGT GTCTTGGGGC CAGAGATCAC CTGCTCCACC ATTGGATCGC GCTTCTTGCC 
GAGTGTCCAA TCACAGCACA GATGTACGAG GACGTGGCGC TCTTAAAGGA CCATTCATTG 
GTGAACTCTC TGATTCGAGT ACTACAGACT CTGCAGGAGT TCAATATCAC TCTGGAAGCT 
TCTCTCGTCA AAGGGATTGG GATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001340162.4
CDS	205..4071
Translation	MSTGFSSSSCRFADYFVICGLDTESGLEPDELSALCQYIQATKFTDGARGKLASANEGENFEQSPLRRTFKSKVLAHYPDNVEWNPFDQDAVGMLCMPKGLSFRTQTDTREPQFHSFIITREDGSRTYGFALTFFEEVTSKQICSAMQTLYHMHNAEQYDILHTPPTPRSPEDPRPTTQQLHASPALSRLQRFNSYDISRDTLYVSKCICLITPMAFAHACRKVLEQLHQAVTSPQPPPLPLESYVYNVLYEVPLPPAGRSLKFSGVYRPVICQRPSTAELPLFDFPLQEVFELLGLENILQLFTCALLEIQILLYSQHYQRLMTVAESITALMFPFQWQHVYVPILPASLLHFLDAPVPYLMGLHSNGQDDRTKLELPQEANLCFVDIDNHFIELPEDLPQFPNKLEFIQEISEVLLAFGLSPEANPQCSEGQSKIRGFRCGDMASDKRNGNLAGSPLNSFLLRENQTIARLQALVKRTGVSLEKLDVKDDASTNKDTKVQCEEEEMKMHQLNIRVREVFANRFSQMFSDYEVFVIQPSQDKESWFSNRDQMQNFDKASFLSDQPEPYLPFLSRFLETQMFASFIDSKILCHDDEDKEHTLRVFDSRVDKIRMINVRTPTLRTSMYQKCTNIDEAEKAIEVRLCKIDHTALHPHLLDMKIGQGRYEPGFFPRLQSDVLSTGPTSNKWAKRSAPAQWRRKDRQKQHAEHLYLDNDQREKYIQEARNLGTTLRQPKLSNLSPSVIAQTNWKFVEGLLKECRNKTKRMLVEKMGREAVELGHGEVSITGVEENTLIASLCDLLERIWSHGLQVKQGKSALWSHLLHYQESKEKRDATPAGLGPPGIIHDTERRKSDASCAMPPLKVSLIQDMRHIQNISEIKTDVGKARAWVRLSMEKKLLSRHLKQLLSDHELTKKLYKRYAFLRCDDEKEQFLYHLLSFNAVDYFCFTNVFTTIMIPYHVVIIPSKKLGGSMFTANPWVCVSGELAETGVLQVPKNTLEITFECQNLGKLTTVQMGHDNSGLYAKWLVECVMVRNGITGHTYKFPCGRWLGKGVDDGSLERILVGELVTNMESDERMCRTPPMQQSPGMMRRFVTISPNSKPKLNTGQIQESVGEAINGIVKHFHKPEKERGSLTLLLCGEYGLVWALEQVFQHGFKSPRLFKNVFIWDFLEKAQVYFESAERSQMTQDENWQMRVRHFCHFMRAINSTPRNIGKDGKFQMLVCLGARDHLLHHWIALLAECPITAQMYEDVALLKDHSLVNSLIRVLQTLQEFNITLEASLVKGIGI

Target ORF information:

RefSeq Version	XM_001340126.7
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio DENN/MADD domain containing 5A (dennd5a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001340126.7

ORF Insert Sequence:

ATGAGCACCG GATTCAGCTC CAGCTCCTGC CGCTTCGCGG ATTATTTCGT GATCTGCGGT 
CTGGACACCG AGAGCGGCTT AGAACCAGAC GAGCTGTCCG CTTTATGCCA GTATATTCAG 
GCTACTAAAT TCACAGATGG CGCCAGAGGA AAGTTGGCGA GTGCAAATGA AGGTGAGAAT 
TTTGAGCAGA GTCCACTGCG GAGAACGTTT AAATCGAAGG TTCTAGCGCA TTATCCTGAC 
AATGTGGAGT GGAACCCTTT TGACCAGGAT GCAGTGGGAA TGCTCTGTAT GCCAAAAGGC 
CTGTCCTTCC GCACTCAAAC AGACACCCGT GAGCCCCAGT TCCACTCCTT CATTATCACC 
AGAGAGGACG GCTCCCGGAC GTACGGCTTT GCCCTCACTT TCTTTGAGGA GGTGACCAGC 
AAACAGATCT GCAGTGCCAT GCAGACACTT TATCACATGC ACAATGCGGA ACAGTATGAT 
ATCCTGCACA CACCACCCAC GCCTCGCAGT CCAGAAGATC CCCGTCCCAC CACGCAACAA 
CTGCACGCCT CCCCTGCTCT GTCCAGGCTG CAGCGCTTCA ACTCATACGA CATCAGCCGG 
GACACGCTGT ACGTGTCCAA ATGCATCTGC CTGATCACCC CCATGGCCTT CGCCCACGCC 
TGCAGGAAGG TTCTGGAGCA GCTGCATCAG GCCGTCACCT CACCTCAGCC CCCGCCGCTG 
CCGCTGGAGA GCTATGTCTA CAATGTCCTC TATGAGGTCC CACTGCCACC AGCCGGACGC 
TCCTTGAAGT TTTCAGGCGT CTACAGGCCA GTGATCTGTC AGAGACCCAG CACAGCCGAG 
CTGCCCCTCT TTGACTTCCC CCTTCAGGAG GTGTTTGAGC TGCTAGGGCT GGAGAACATC 
TTGCAGCTCT TCACCTGTGC GCTGCTGGAG ATCCAGATCC TTCTGTACTC ACAACATTAC 
CAGCGGCTGA TGACGGTGGC GGAGAGCATC ACAGCCTTAA TGTTCCCCTT TCAGTGGCAG 
CATGTGTACG TGCCCATCCT GCCCGCTTCC CTGCTCCACT TCCTGGACGC TCCTGTGCCG 
TACCTCATGG GTCTGCACTC TAACGGCCAG GATGACCGCA CCAAACTGGA GCTTCCCCAA 
GAGGCCAATC TCTGCTTTGT CGATATTGAC AACCACTTCA TCGAGCTACC CGAAGACCTG 
CCTCAGTTCC CCAACAAGCT GGAGTTTATC CAGGAGATCT CTGAGGTTCT GCTGGCGTTT 
GGCTTGTCTC CAGAAGCGAA CCCACAATGC AGCGAGGGCC AGAGCAAGAT CAGGGGCTTC 
CGGTGTGGCG ACATGGCCTC CGACAAGCGC AACGGGAACC TGGCCGGCTC GCCGCTCAAT 
TCGTTCCTGC TGAGAGAAAA CCAAACCATC GCTCGCCTGC AAGCGCTGGT CAAGAGGACT 
GGAGTCAGTC TGGAGAAGTT GGACGTGAAG GACGACGCAA GCACTAATAA GGATACGAAA 
GTGCAGTGTG AAGAAGAAGA GATGAAAATG CACCAGCTGA ACATTCGGGT GCGAGAGGTC 
TTCGCCAACC GTTTCTCTCA GATGTTCTCG GATTATGAGG TGTTTGTTAT TCAGCCCAGC 
CAAGACAAAG AGTCCTGGTT CAGCAACCGA GACCAGATGC AGAACTTTGA CAAGGCCTCC 
TTCCTGTCAG ATCAGCCCGA ACCGTACCTG CCGTTCCTCT CCCGCTTTCT GGAGACGCAG 
ATGTTTGCCT CTTTTATCGA CAGTAAGATT CTGTGCCACG ATGACGAGGA CAAAGAGCAC 
ACACTGCGAG TCTTTGATTC CCGTGTTGAC AAGATCCGCA TGATAAATGT CAGGACACCC 
ACCCTGCGGA CCTCCATGTA CCAGAAATGC ACCAATATCG ATGAAGCAGA AAAAGCCATA 
GAGGTGAGAC TATGTAAGAT CGACCACACG GCTCTGCATC CCCATCTGCT GGACATGAAG 
ATCGGTCAGG GCCGCTATGA GCCAGGCTTT TTCCCTCGCT TACAGTCTGA TGTGCTCTCC 
ACTGGACCCA CCAGCAACAA GTGGGCGAAG CGCAGTGCAC CGGCCCAATG GCGGAGGAAA 
GACCGACAGA AACAGCACGC CGAACATCTC TATCTGGACA ATGACCAGAG AGAGAAATAC 
ATCCAGGAAG CCAGAAACCT TGGCACCACA CTCCGTCAGC CAAAACTCTC CAACCTGTCG 
CCTTCGGTCA TTGCACAGAC CAACTGGAAG TTTGTTGAGG GACTGTTGAA GGAGTGCAGA 
AATAAGACAA AGCGCATGCT GGTGGAGAAG ATGGGACGGG AGGCAGTGGA GCTGGGCCAC 
GGTGAGGTGA GCATCACCGG TGTGGAGGAA AACACACTCA TCGCCAGCCT GTGTGACCTG 
CTGGAGCGAA TCTGGAGCCA CGGCCTACAG GTCAAACAGG GAAAATCCGC CCTGTGGTCG 
CACCTTTTAC ATTATCAAGA GAGTAAAGAG AAGAGAGACG CTACTCCAGC AGGCCTCGGC 
CCTCCTGGTA TCATTCATGA CACAGAGAGA CGGAAGTCTG ATGCTAGCTG TGCGATGCCG 
CCTCTCAAAG TCTCCTTGAT TCAGGACATG AGGCACATCC AGAACATCAG CGAGATCAAG 
ACGGACGTTG GCAAGGCCAG GGCTTGGGTG CGTCTCTCCA TGGAGAAGAA ACTGCTGTCC 
AGACACCTGA AGCAACTGCT GTCTGACCAC GAGCTCACCA AGAAACTCTA TAAGCGATAT 
GCTTTTCTGC GCTGTGATGA CGAGAAGGAG CAGTTCCTCT ACCATCTCCT CTCTTTCAAC 
GCTGTGGACT ACTTCTGCTT CACTAATGTC TTCACCACAA TTATGATTCC ATACCATGTG 
GTCATCATTC CTAGTAAGAA ACTCGGTGGC TCTATGTTCA CAGCCAACCC CTGGGTGTGT 
GTCTCAGGGG AACTAGCCGA GACTGGGGTC CTGCAGGTCC CTAAAAACAC ACTGGAGATT 
ACATTTGAGT GCCAGAATCT GGGGAAGCTG ACCACTGTAC AGATGGGCCA TGATAACTCA 
GGGCTGTATG CTAAATGGCT AGTGGAGTGC GTAATGGTCC GCAATGGGAT TACAGGACAC 
ACCTATAAGT TTCCATGTGG CCGTTGGCTG GGTAAAGGAG TAGATGATGG CAGCTTGGAG 
AGGATCCTGG TGGGCGAATT GGTGACTAAC ATGGAGAGCG ATGAAAGAAT GTGCCGGACT 
CCTCCGATGC AGCAATCCCC CGGGATGATG CGAAGATTTG TCACCATTTC GCCAAACAGC 
AAACCAAAGT TAAACACGGG CCAGATCCAG GAAAGTGTGG GAGAGGCGAT TAACGGGATC 
GTCAAACACT TCCATAAGCC GGAGAAAGAG AGAGGAAGCC TCACGTTGCT TCTGTGTGGG 
GAGTATGGTT TGGTTTGGGC TCTGGAGCAG GTGTTTCAGC ATGGCTTCAA GTCACCGCGA 
CTGTTCAAGA ACGTCTTCAT TTGGGACTTT CTAGAAAAGG CGCAGGTTTA CTTTGAGAGT 
GCGGAGCGGA GTCAGATGAC CCAGGATGAG AACTGGCAGA TGAGGGTCCG TCATTTCTGC 
CATTTCATGC GCGCCATCAA CAGCACACCC AGAAACATCG GCAAAGATGG GAAGTTTCAG 
ATGCTTGTGT GTCTTGGGGC CAGAGATCAC CTGCTCCACC ATTGGATCGC GCTTCTTGCC 
GAGTGTCCAA TCACAGCACA GATGTACGAG GACGTGGCGC TCTTAAAGGA CCATTCATTG 
GTGAACTCTC TGATTCGAGT ACTACAGACT CTGCAGGAGT TCAATATCAC TCTGGAAGCT 
TCTCTCGTCA AAGGGATTGG GATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dennd5a ( XM_005166611.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005166668.1 Danio rerio DENN/MADD domain containing 5A (dennd5a), transcript variant X4, mRNA.

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CloneID	ODa13599
Clone ID Related Accession (Same CDS sequence)	XM_005166611.3 , XM_005166611.4
Accession Version	XM_005166611.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3795bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	DENN domain-containing protein 5A isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005166611.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCACCG GATTCAGCTC CAGCTCCTGC CGCTTCGCGG ATTATTTCGT GATCTGCGGT 
CTGGACACCG AGAGCGGCTT AGAACCAGAC GAGCTGTCCG GTGAGAATTT TGAGCAGAGT 
CCACTGCGGA GAACGTTTAA ATCGAAGGTT CTAGCGCATT ATCCTGACAA TGTGGAGTGG 
AACCCTTTTG ACCAGGATGC AGTGGGAATG CTCTGTATGC CAAAAGGCCT GTCCTTCCGC 
ACTCAAACAG ACACCCGTGA GCCCCAGTTC CACTCCTTCA TTATCACCAG AGAGGACGGC 
TCCCGGACGT ACGGCTTTGC CCTCACTTTC TTTGAGGAGG TGACCAGCAA ACAGATCTGC 
AGTGCCATGC AGACACTTTA TCACATGCAC AATGCGGAAC AGTATGATAT CCTGCACACA 
CCACCCACGC CTCGCAGTCC AGAAGATCCC CGTCCCACCA CGCAACAACT GCACGCCTCC 
CCTGCTCTGT CCAGGCTGCA GCGCTTCAAC TCATACGACA TCAGCCGGGA CACGCTGTAC 
GTGTCCAAAT GCATCTGCCT GATCACCCCC ATGGCCTTCG CCCACGCCTG CAGGAAGGTT 
CTGGAGCAGC TGCATCAGGC CGTCACCTCA CCTCAGCCCC CGCCGCTGCC GCTGGAGAGC 
TATGTCTACA ATGTCCTCTA TGAGGTCCCA CTGCCACCAG CCGGACGCTC CTTGAAGTTT 
TCAGGCGTCT ACAGGCCAGT GATCTGTCAG AGACCCAGCA CAGCCGAGCT GCCCCTCTTT 
GACTTCCCCC TTCAGGAGGT GTTTGAGCTG CTAGGGCTGG AGAACATCTT GCAGCTCTTC 
ACCTGTGCGC TGCTGGAGAT CCAGATCCTT CTGTACTCAC AACATTACCA GCGGCTGATG 
ACGGTGGCGG AGAGCATCAC AGCCTTAATG TTCCCCTTTC AGTGGCAGCA TGTGTACGTG 
CCCATCCTGC CCGCTTCCCT GCTCCACTTC CTGGACGCTC CTGTGCCGTA CCTCATGGGT 
CTGCACTCTA ACGGCCAGGA TGACCGCACC AAACTGGAGC TTCCCCAAGA GGCCAATCTC 
TGCTTTGTCG ATATTGACAA CCACTTCATC GAGCTACCCG AAGACCTGCC TCAGTTCCCC 
AACAAGCTGG AGTTTATCCA GGAGATCTCT GAGGTTCTGC TGGCGTTTGG CTTGTCTCCA 
GAAGCGAACC CACAATGCAG CGAGGGCCAG AGCAAGATCA GGGGCTTCCG GTGTGGCGAC 
ATGGCCTCCG ACAAGCGCAA CGGGAACCTG GCCGGCTCGC CGCTCAATTC GTTCCTGCTG 
AGAGAAAACC AAACCATCGC TCGCCTGCAA GCGCTGGTCA AGAGGACTGG AGTCAGTCTG 
GAGAAGTTGG ACGTGAAGGA CGACGCAAGC ACTAATAAGG ATACGAAAGT GCAGTGTGAA 
GAAGAAGAGA TGAAAATGCA CCAGCTGAAC ATTCGGGTGC GAGAGGTCTT CGCCAACCGT 
TTCTCTCAGA TGTTCTCGGA TTATGAGGTG TTTGTTATTC AGCCCAGCCA AGACAAAGAG 
TCCTGGTTCA GCAACCGAGA CCAGATGCAG AACTTTGACA AGGCCTCCTT CCTGTCAGAT 
CAGCCCGAAC CGTACCTGCC GTTCCTCTCC CGCTTTCTGG AGACGCAGAT GTTTGCCTCT 
TTTATCGACA GTAAGATTCT GTGCCACGAT GACGAGGACA AAGAGCACAC ACTGCGAGTC 
TTTGATTCCC GTGTTGACAA GATCCGCATG ATAAATGTCA GGACACCCAC CCTGCGGACC 
TCCATGTACC AGAAATGCAC CAATATCGAT GAAGCAGAAA AAGCCATAGA GGTGAGACTA 
TGTAAGATCG ACCACACGGC TCTGCATCCC CATCTGCTGG ACATGAAGAT CGGTCAGGGC 
CGCTATGAGC CAGGCTTTTT CCCTCGCTTA CAGTCTGATG TGCTCTCCAC TGGACCCACC 
AGCAACAAGT GGGCGAAGCG CAGTGCACCG GCCCAATGGC GGAGGAAAGA CCGACAGAAA 
CAGCACGCCG AACATCTCTA TCTGGACAAT GACCAGAGAG AGAAATACAT CCAGGAAGCC 
AGAAACCTTG GCACCACACT CCGTCAGCCA AAACTCTCCA ACCTGTCGCC TTCGGTCATT 
GCACAGACCA ACTGGAAGTT TGTTGAGGGA CTGTTGAAGG AGTGCAGAAA TAAGACAAAG 
CGCATGCTGG TGGAGAAGAT GGGACGGGAG GCAGTGGAGC TGGGCCACGG TGAGGTGAGC 
ATCACCGGTG TGGAGGAAAA CACACTCATC GCCAGCCTGT GTGACCTGCT GGAGCGAATC 
TGGAGCCACG GCCTACAGGT CAAACAGGGA AAATCCGCCC TGTGGTCGCA CCTTTTACAT 
TATCAAGAGA GTAAAGAGAA GAGAGACGCT ACTCCAGCAG GCCTCGGCCC TCCTGGTATC 
ATTCATGACA CAGAGAGACG GAAGTCTGAT GCTAGCTGTG CGATGCCGCC TCTCAAAGTC 
TCCTTGATTC AGGACATGAG GCACATCCAG AACATCAGCG AGATCAAGAC GGACGTTGGC 
AAGGCCAGGG CTTGGGTGCG TCTCTCCATG GAGAAGAAAC TGCTGTCCAG ACACCTGAAG 
CAACTGCTGT CTGACCACGA GCTCACCAAG AAACTCTATA AGCGATATGC TTTTCTGCGC 
TGTGATGACG AGAAGGAGCA GTTCCTCTAC CATCTCCTCT CTTTCAACGC TGTGGACTAC 
TTCTGCTTCA CTAATGTCTT CACCACAATT ATGATTCCAT ACCATGTGGT CATCATTCCT 
AGTAAGAAAC TCGGTGGCTC TATGTTCACA GCCAACCCCT GGGTGTGTGT CTCAGGGGAA 
CTAGCCGAGA CTGGGGTCCT GCAGGTCCCT AAAAACACAC TGGAGATTAC ATTTGAGTGC 
CAGAATCTGG GGAAGCTGAC CACTGTACAG ATGGGCCATG ATAACTCAGG GCTGTATGCT 
AAATGGCTAG TGGAGTGCGT AATGGTCCGC AATGGGATTA CAGGACACAC CTATAAGTTT 
CCATGTGGCC GTTGGCTGGG TAAAGGAGTA GATGATGGCA GCTTGGAGAG GATCCTGGTG 
GGCGAATTGG TGACTAACAT GGAGAGCGAT GAAAGAATGT GCCGGACTCC TCCGATGCAG 
CAATCCCCCG GGATGATGCG AAGATTTGTC ACCATTTCGC CAAACAGCAA ACCAAAGTTA 
AACACGGGCC AGATCCAGGA AAGTGTGGGA GAGGCGATTA ACGGGATCGT CAAACACTTC 
CATAAGCCGG AGAAAGAGAG AGGAAGCCTC ACGTTGCTTC TGTGTGGGGA GTATGGTTTG 
GTTTGGGCTC TGGAGCAGGT GTTTCAGCAT GGCTTCAAGT CACCGCGACT GTTCAAGAAC 
GTCTTCATTT GGGACTTTCT AGAAAAGGCG CAGGTTTACT TTGAGAGTGC GGAGCGGAGT 
CAGATGACCC AGGATGAGAA CTGGCAGATG AGGGTCCGTC ATTTCTGCCA TTTCATGCGC 
GCCATCAACA GCACACCCAG AAACATCGGC AAAGATGGGA AGTTTCAGAT GCTTGTGTGT 
CTTGGGGCCA GAGATCACCT GCTCCACCAT TGGATCGCGC TTCTTGCCGA GTGTCCAATC 
ACAGCACAGA TGTACGAGGA CGTGGCGCTC TTAAAGGACC ATTCATTGGT GAACTCTCTG 
ATTCGAGTAC TACAGACTCT GCAGGAGTTC AATATCACTC TGGAAGCTTC TCTCGTCAAA 
GGGATTGGGA TATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005166668.1
CDS	205..3999
Translation	MSTGFSSSSCRFADYFVICGLDTESGLEPDELSGENFEQSPLRRTFKSKVLAHYPDNVEWNPFDQDAVGMLCMPKGLSFRTQTDTREPQFHSFIITREDGSRTYGFALTFFEEVTSKQICSAMQTLYHMHNAEQYDILHTPPTPRSPEDPRPTTQQLHASPALSRLQRFNSYDISRDTLYVSKCICLITPMAFAHACRKVLEQLHQAVTSPQPPPLPLESYVYNVLYEVPLPPAGRSLKFSGVYRPVICQRPSTAELPLFDFPLQEVFELLGLENILQLFTCALLEIQILLYSQHYQRLMTVAESITALMFPFQWQHVYVPILPASLLHFLDAPVPYLMGLHSNGQDDRTKLELPQEANLCFVDIDNHFIELPEDLPQFPNKLEFIQEISEVLLAFGLSPEANPQCSEGQSKIRGFRCGDMASDKRNGNLAGSPLNSFLLRENQTIARLQALVKRTGVSLEKLDVKDDASTNKDTKVQCEEEEMKMHQLNIRVREVFANRFSQMFSDYEVFVIQPSQDKESWFSNRDQMQNFDKASFLSDQPEPYLPFLSRFLETQMFASFIDSKILCHDDEDKEHTLRVFDSRVDKIRMINVRTPTLRTSMYQKCTNIDEAEKAIEVRLCKIDHTALHPHLLDMKIGQGRYEPGFFPRLQSDVLSTGPTSNKWAKRSAPAQWRRKDRQKQHAEHLYLDNDQREKYIQEARNLGTTLRQPKLSNLSPSVIAQTNWKFVEGLLKECRNKTKRMLVEKMGREAVELGHGEVSITGVEENTLIASLCDLLERIWSHGLQVKQGKSALWSHLLHYQESKEKRDATPAGLGPPGIIHDTERRKSDASCAMPPLKVSLIQDMRHIQNISEIKTDVGKARAWVRLSMEKKLLSRHLKQLLSDHELTKKLYKRYAFLRCDDEKEQFLYHLLSFNAVDYFCFTNVFTTIMIPYHVVIIPSKKLGGSMFTANPWVCVSGELAETGVLQVPKNTLEITFECQNLGKLTTVQMGHDNSGLYAKWLVECVMVRNGITGHTYKFPCGRWLGKGVDDGSLERILVGELVTNMESDERMCRTPPMQQSPGMMRRFVTISPNSKPKLNTGQIQESVGEAINGIVKHFHKPEKERGSLTLLLCGEYGLVWALEQVFQHGFKSPRLFKNVFIWDFLEKAQVYFESAERSQMTQDENWQMRVRHFCHFMRAINSTPRNIGKDGKFQMLVCLGARDHLLHHWIALLAECPITAQMYEDVALLKDHSLVNSLIRVLQTLQEFNITLEASLVKGIGI

Target ORF information:

RefSeq Version	XM_005166611.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio DENN/MADD domain containing 5A (dennd5a), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005166611.3

ORF Insert Sequence:

ATGAGCACCG GATTCAGCTC CAGCTCCTGC CGCTTCGCGG ATTATTTCGT GATCTGCGGT 
CTGGACACCG AGAGCGGCTT AGAACCAGAC GAGCTGTCCG GTGAGAATTT TGAGCAGAGT 
CCACTGCGGA GAACGTTTAA ATCGAAGGTT CTAGCGCATT ATCCTGACAA TGTGGAGTGG 
AACCCTTTTG ACCAGGATGC AGTGGGAATG CTCTGTATGC CAAAAGGCCT GTCCTTCCGC 
ACTCAAACAG ACACCCGTGA GCCCCAGTTC CACTCCTTCA TTATCACCAG AGAGGACGGC 
TCCCGGACGT ACGGCTTTGC CCTCACTTTC TTTGAGGAGG TGACCAGCAA ACAGATCTGC 
AGTGCCATGC AGACACTTTA TCACATGCAC AATGCGGAAC AGTATGATAT CCTGCACACA 
CCACCCACGC CTCGCAGTCC AGAAGATCCC CGTCCCACCA CGCAACAACT GCACGCCTCC 
CCTGCTCTGT CCAGGCTGCA GCGCTTCAAC TCATACGACA TCAGCCGGGA CACGCTGTAC 
GTGTCCAAAT GCATCTGCCT GATCACCCCC ATGGCCTTCG CCCACGCCTG CAGGAAGGTT 
CTGGAGCAGC TGCATCAGGC CGTCACCTCA CCTCAGCCCC CGCCGCTGCC GCTGGAGAGC 
TATGTCTACA ATGTCCTCTA TGAGGTCCCA CTGCCACCAG CCGGACGCTC CTTGAAGTTT 
TCAGGCGTCT ACAGGCCAGT GATCTGTCAG AGACCCAGCA CAGCCGAGCT GCCCCTCTTT 
GACTTCCCCC TTCAGGAGGT GTTTGAGCTG CTAGGGCTGG AGAACATCTT GCAGCTCTTC 
ACCTGTGCGC TGCTGGAGAT CCAGATCCTT CTGTACTCAC AACATTACCA GCGGCTGATG 
ACGGTGGCGG AGAGCATCAC AGCCTTAATG TTCCCCTTTC AGTGGCAGCA TGTGTACGTG 
CCCATCCTGC CCGCTTCCCT GCTCCACTTC CTGGACGCTC CTGTGCCGTA CCTCATGGGT 
CTGCACTCTA ACGGCCAGGA TGACCGCACC AAACTGGAGC TTCCCCAAGA GGCCAATCTC 
TGCTTTGTCG ATATTGACAA CCACTTCATC GAGCTACCCG AAGACCTGCC TCAGTTCCCC 
AACAAGCTGG AGTTTATCCA GGAGATCTCT GAGGTTCTGC TGGCGTTTGG CTTGTCTCCA 
GAAGCGAACC CACAATGCAG CGAGGGCCAG AGCAAGATCA GGGGCTTCCG GTGTGGCGAC 
ATGGCCTCCG ACAAGCGCAA CGGGAACCTG GCCGGCTCGC CGCTCAATTC GTTCCTGCTG 
AGAGAAAACC AAACCATCGC TCGCCTGCAA GCGCTGGTCA AGAGGACTGG AGTCAGTCTG 
GAGAAGTTGG ACGTGAAGGA CGACGCAAGC ACTAATAAGG ATACGAAAGT GCAGTGTGAA 
GAAGAAGAGA TGAAAATGCA CCAGCTGAAC ATTCGGGTGC GAGAGGTCTT CGCCAACCGT 
TTCTCTCAGA TGTTCTCGGA TTATGAGGTG TTTGTTATTC AGCCCAGCCA AGACAAAGAG 
TCCTGGTTCA GCAACCGAGA CCAGATGCAG AACTTTGACA AGGCCTCCTT CCTGTCAGAT 
CAGCCCGAAC CGTACCTGCC GTTCCTCTCC CGCTTTCTGG AGACGCAGAT GTTTGCCTCT 
TTTATCGACA GTAAGATTCT GTGCCACGAT GACGAGGACA AAGAGCACAC ACTGCGAGTC 
TTTGATTCCC GTGTTGACAA GATCCGCATG ATAAATGTCA GGACACCCAC CCTGCGGACC 
TCCATGTACC AGAAATGCAC CAATATCGAT GAAGCAGAAA AAGCCATAGA GGTGAGACTA 
TGTAAGATCG ACCACACGGC TCTGCATCCC CATCTGCTGG ACATGAAGAT CGGTCAGGGC 
CGCTATGAGC CAGGCTTTTT CCCTCGCTTA CAGTCTGATG TGCTCTCCAC TGGACCCACC 
AGCAACAAGT GGGCGAAGCG CAGTGCACCG GCCCAATGGC GGAGGAAAGA CCGACAGAAA 
CAGCACGCCG AACATCTCTA TCTGGACAAT GACCAGAGAG AGAAATACAT CCAGGAAGCC 
AGAAACCTTG GCACCACACT CCGTCAGCCA AAACTCTCCA ACCTGTCGCC TTCGGTCATT 
GCACAGACCA ACTGGAAGTT TGTTGAGGGA CTGTTGAAGG AGTGCAGAAA TAAGACAAAG 
CGCATGCTGG TGGAGAAGAT GGGACGGGAG GCAGTGGAGC TGGGCCACGG TGAGGTGAGC 
ATCACCGGTG TGGAGGAAAA CACACTCATC GCCAGCCTGT GTGACCTGCT GGAGCGAATC 
TGGAGCCACG GCCTACAGGT CAAACAGGGA AAATCCGCCC TGTGGTCGCA CCTTTTACAT 
TATCAAGAGA GTAAAGAGAA GAGAGACGCT ACTCCAGCAG GCCTCGGCCC TCCTGGTATC 
ATTCATGACA CAGAGAGACG GAAGTCTGAT GCTAGCTGTG CGATGCCGCC TCTCAAAGTC 
TCCTTGATTC AGGACATGAG GCACATCCAG AACATCAGCG AGATCAAGAC GGACGTTGGC 
AAGGCCAGGG CTTGGGTGCG TCTCTCCATG GAGAAGAAAC TGCTGTCCAG ACACCTGAAG 
CAACTGCTGT CTGACCACGA GCTCACCAAG AAACTCTATA AGCGATATGC TTTTCTGCGC 
TGTGATGACG AGAAGGAGCA GTTCCTCTAC CATCTCCTCT CTTTCAACGC TGTGGACTAC 
TTCTGCTTCA CTAATGTCTT CACCACAATT ATGATTCCAT ACCATGTGGT CATCATTCCT 
AGTAAGAAAC TCGGTGGCTC TATGTTCACA GCCAACCCCT GGGTGTGTGT CTCAGGGGAA 
CTAGCCGAGA CTGGGGTCCT GCAGGTCCCT AAAAACACAC TGGAGATTAC ATTTGAGTGC 
CAGAATCTGG GGAAGCTGAC CACTGTACAG ATGGGCCATG ATAACTCAGG GCTGTATGCT 
AAATGGCTAG TGGAGTGCGT AATGGTCCGC AATGGGATTA CAGGACACAC CTATAAGTTT 
CCATGTGGCC GTTGGCTGGG TAAAGGAGTA GATGATGGCA GCTTGGAGAG GATCCTGGTG 
GGCGAATTGG TGACTAACAT GGAGAGCGAT GAAAGAATGT GCCGGACTCC TCCGATGCAG 
CAATCCCCCG GGATGATGCG AAGATTTGTC ACCATTTCGC CAAACAGCAA ACCAAAGTTA 
AACACGGGCC AGATCCAGGA AAGTGTGGGA GAGGCGATTA ACGGGATCGT CAAACACTTC 
CATAAGCCGG AGAAAGAGAG AGGAAGCCTC ACGTTGCTTC TGTGTGGGGA GTATGGTTTG 
GTTTGGGCTC TGGAGCAGGT GTTTCAGCAT GGCTTCAAGT CACCGCGACT GTTCAAGAAC 
GTCTTCATTT GGGACTTTCT AGAAAAGGCG CAGGTTTACT TTGAGAGTGC GGAGCGGAGT 
CAGATGACCC AGGATGAGAA CTGGCAGATG AGGGTCCGTC ATTTCTGCCA TTTCATGCGC 
GCCATCAACA GCACACCCAG AAACATCGGC AAAGATGGGA AGTTTCAGAT GCTTGTGTGT 
CTTGGGGCCA GAGATCACCT GCTCCACCAT TGGATCGCGC TTCTTGCCGA GTGTCCAATC 
ACAGCACAGA TGTACGAGGA CGTGGCGCTC TTAAAGGACC ATTCATTGGT GAACTCTCTG 
ATTCGAGTAC TACAGACTCT GCAGGAGTTC AATATCACTC TGGAAGCTTC TCTCGTCAAA 
GGGATTGGGA TATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dennd5a ( XM_005166611.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005166668.1 Danio rerio DENN/MADD domain containing 5A (dennd5a), transcript variant X4, mRNA.

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CloneID	ODa13599
Clone ID Related Accession (Same CDS sequence)	XM_005166611.3 , XM_005166611.4
Accession Version	XM_005166611.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3795bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	DENN domain-containing protein 5A isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005166611.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCACCG GATTCAGCTC CAGCTCCTGC CGCTTCGCGG ATTATTTCGT GATCTGCGGT 
CTGGACACCG AGAGCGGCTT AGAACCAGAC GAGCTGTCCG GTGAGAATTT TGAGCAGAGT 
CCACTGCGGA GAACGTTTAA ATCGAAGGTT CTAGCGCATT ATCCTGACAA TGTGGAGTGG 
AACCCTTTTG ACCAGGATGC AGTGGGAATG CTCTGTATGC CAAAAGGCCT GTCCTTCCGC 
ACTCAAACAG ACACCCGTGA GCCCCAGTTC CACTCCTTCA TTATCACCAG AGAGGACGGC 
TCCCGGACGT ACGGCTTTGC CCTCACTTTC TTTGAGGAGG TGACCAGCAA ACAGATCTGC 
AGTGCCATGC AGACACTTTA TCACATGCAC AATGCGGAAC AGTATGATAT CCTGCACACA 
CCACCCACGC CTCGCAGTCC AGAAGATCCC CGTCCCACCA CGCAACAACT GCACGCCTCC 
CCTGCTCTGT CCAGGCTGCA GCGCTTCAAC TCATACGACA TCAGCCGGGA CACGCTGTAC 
GTGTCCAAAT GCATCTGCCT GATCACCCCC ATGGCCTTCG CCCACGCCTG CAGGAAGGTT 
CTGGAGCAGC TGCATCAGGC CGTCACCTCA CCTCAGCCCC CGCCGCTGCC GCTGGAGAGC 
TATGTCTACA ATGTCCTCTA TGAGGTCCCA CTGCCACCAG CCGGACGCTC CTTGAAGTTT 
TCAGGCGTCT ACAGGCCAGT GATCTGTCAG AGACCCAGCA CAGCCGAGCT GCCCCTCTTT 
GACTTCCCCC TTCAGGAGGT GTTTGAGCTG CTAGGGCTGG AGAACATCTT GCAGCTCTTC 
ACCTGTGCGC TGCTGGAGAT CCAGATCCTT CTGTACTCAC AACATTACCA GCGGCTGATG 
ACGGTGGCGG AGAGCATCAC AGCCTTAATG TTCCCCTTTC AGTGGCAGCA TGTGTACGTG 
CCCATCCTGC CCGCTTCCCT GCTCCACTTC CTGGACGCTC CTGTGCCGTA CCTCATGGGT 
CTGCACTCTA ACGGCCAGGA TGACCGCACC AAACTGGAGC TTCCCCAAGA GGCCAATCTC 
TGCTTTGTCG ATATTGACAA CCACTTCATC GAGCTACCCG AAGACCTGCC TCAGTTCCCC 
AACAAGCTGG AGTTTATCCA GGAGATCTCT GAGGTTCTGC TGGCGTTTGG CTTGTCTCCA 
GAAGCGAACC CACAATGCAG CGAGGGCCAG AGCAAGATCA GGGGCTTCCG GTGTGGCGAC 
ATGGCCTCCG ACAAGCGCAA CGGGAACCTG GCCGGCTCGC CGCTCAATTC GTTCCTGCTG 
AGAGAAAACC AAACCATCGC TCGCCTGCAA GCGCTGGTCA AGAGGACTGG AGTCAGTCTG 
GAGAAGTTGG ACGTGAAGGA CGACGCAAGC ACTAATAAGG ATACGAAAGT GCAGTGTGAA 
GAAGAAGAGA TGAAAATGCA CCAGCTGAAC ATTCGGGTGC GAGAGGTCTT CGCCAACCGT 
TTCTCTCAGA TGTTCTCGGA TTATGAGGTG TTTGTTATTC AGCCCAGCCA AGACAAAGAG 
TCCTGGTTCA GCAACCGAGA CCAGATGCAG AACTTTGACA AGGCCTCCTT CCTGTCAGAT 
CAGCCCGAAC CGTACCTGCC GTTCCTCTCC CGCTTTCTGG AGACGCAGAT GTTTGCCTCT 
TTTATCGACA GTAAGATTCT GTGCCACGAT GACGAGGACA AAGAGCACAC ACTGCGAGTC 
TTTGATTCCC GTGTTGACAA GATCCGCATG ATAAATGTCA GGACACCCAC CCTGCGGACC 
TCCATGTACC AGAAATGCAC CAATATCGAT GAAGCAGAAA AAGCCATAGA GGTGAGACTA 
TGTAAGATCG ACCACACGGC TCTGCATCCC CATCTGCTGG ACATGAAGAT CGGTCAGGGC 
CGCTATGAGC CAGGCTTTTT CCCTCGCTTA CAGTCTGATG TGCTCTCCAC TGGACCCACC 
AGCAACAAGT GGGCGAAGCG CAGTGCACCG GCCCAATGGC GGAGGAAAGA CCGACAGAAA 
CAGCACGCCG AACATCTCTA TCTGGACAAT GACCAGAGAG AGAAATACAT CCAGGAAGCC 
AGAAACCTTG GCACCACACT CCGTCAGCCA AAACTCTCCA ACCTGTCGCC TTCGGTCATT 
GCACAGACCA ACTGGAAGTT TGTTGAGGGA CTGTTGAAGG AGTGCAGAAA TAAGACAAAG 
CGCATGCTGG TGGAGAAGAT GGGACGGGAG GCAGTGGAGC TGGGCCACGG TGAGGTGAGC 
ATCACCGGTG TGGAGGAAAA CACACTCATC GCCAGCCTGT GTGACCTGCT GGAGCGAATC 
TGGAGCCACG GCCTACAGGT CAAACAGGGA AAATCCGCCC TGTGGTCGCA CCTTTTACAT 
TATCAAGAGA GTAAAGAGAA GAGAGACGCT ACTCCAGCAG GCCTCGGCCC TCCTGGTATC 
ATTCATGACA CAGAGAGACG GAAGTCTGAT GCTAGCTGTG CGATGCCGCC TCTCAAAGTC 
TCCTTGATTC AGGACATGAG GCACATCCAG AACATCAGCG AGATCAAGAC GGACGTTGGC 
AAGGCCAGGG CTTGGGTGCG TCTCTCCATG GAGAAGAAAC TGCTGTCCAG ACACCTGAAG 
CAACTGCTGT CTGACCACGA GCTCACCAAG AAACTCTATA AGCGATATGC TTTTCTGCGC 
TGTGATGACG AGAAGGAGCA GTTCCTCTAC CATCTCCTCT CTTTCAACGC TGTGGACTAC 
TTCTGCTTCA CTAATGTCTT CACCACAATT ATGATTCCAT ACCATGTGGT CATCATTCCT 
AGTAAGAAAC TCGGTGGCTC TATGTTCACA GCCAACCCCT GGGTGTGTGT CTCAGGGGAA 
CTAGCCGAGA CTGGGGTCCT GCAGGTCCCT AAAAACACAC TGGAGATTAC ATTTGAGTGC 
CAGAATCTGG GGAAGCTGAC CACTGTACAG ATGGGCCATG ATAACTCAGG GCTGTATGCT 
AAATGGCTAG TGGAGTGCGT AATGGTCCGC AATGGGATTA CAGGACACAC CTATAAGTTT 
CCATGTGGCC GTTGGCTGGG TAAAGGAGTA GATGATGGCA GCTTGGAGAG GATCCTGGTG 
GGCGAATTGG TGACTAACAT GGAGAGCGAT GAAAGAATGT GCCGGACTCC TCCGATGCAG 
CAATCCCCCG GGATGATGCG AAGATTTGTC ACCATTTCGC CAAACAGCAA ACCAAAGTTA 
AACACGGGCC AGATCCAGGA AAGTGTGGGA GAGGCGATTA ACGGGATCGT CAAACACTTC 
CATAAGCCGG AGAAAGAGAG AGGAAGCCTC ACGTTGCTTC TGTGTGGGGA GTATGGTTTG 
GTTTGGGCTC TGGAGCAGGT GTTTCAGCAT GGCTTCAAGT CACCGCGACT GTTCAAGAAC 
GTCTTCATTT GGGACTTTCT AGAAAAGGCG CAGGTTTACT TTGAGAGTGC GGAGCGGAGT 
CAGATGACCC AGGATGAGAA CTGGCAGATG AGGGTCCGTC ATTTCTGCCA TTTCATGCGC 
GCCATCAACA GCACACCCAG AAACATCGGC AAAGATGGGA AGTTTCAGAT GCTTGTGTGT 
CTTGGGGCCA GAGATCACCT GCTCCACCAT TGGATCGCGC TTCTTGCCGA GTGTCCAATC 
ACAGCACAGA TGTACGAGGA CGTGGCGCTC TTAAAGGACC ATTCATTGGT GAACTCTCTG 
ATTCGAGTAC TACAGACTCT GCAGGAGTTC AATATCACTC TGGAAGCTTC TCTCGTCAAA 
GGGATTGGGA TATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005166668.1
CDS	210..4004
Translation	MSTGFSSSSCRFADYFVICGLDTESGLEPDELSGENFEQSPLRRTFKSKVLAHYPDNVEWNPFDQDAVGMLCMPKGLSFRTQTDTREPQFHSFIITREDGSRTYGFALTFFEEVTSKQICSAMQTLYHMHNAEQYDILHTPPTPRSPEDPRPTTQQLHASPALSRLQRFNSYDISRDTLYVSKCICLITPMAFAHACRKVLEQLHQAVTSPQPPPLPLESYVYNVLYEVPLPPAGRSLKFSGVYRPVICQRPSTAELPLFDFPLQEVFELLGLENILQLFTCALLEIQILLYSQHYQRLMTVAESITALMFPFQWQHVYVPILPASLLHFLDAPVPYLMGLHSNGQDDRTKLELPQEANLCFVDIDNHFIELPEDLPQFPNKLEFIQEISEVLLAFGLSPEANPQCSEGQSKIRGFRCGDMASDKRNGNLAGSPLNSFLLRENQTIARLQALVKRTGVSLEKLDVKDDASTNKDTKVQCEEEEMKMHQLNIRVREVFANRFSQMFSDYEVFVIQPSQDKESWFSNRDQMQNFDKASFLSDQPEPYLPFLSRFLETQMFASFIDSKILCHDDEDKEHTLRVFDSRVDKIRMINVRTPTLRTSMYQKCTNIDEAEKAIEVRLCKIDHTALHPHLLDMKIGQGRYEPGFFPRLQSDVLSTGPTSNKWAKRSAPAQWRRKDRQKQHAEHLYLDNDQREKYIQEARNLGTTLRQPKLSNLSPSVIAQTNWKFVEGLLKECRNKTKRMLVEKMGREAVELGHGEVSITGVEENTLIASLCDLLERIWSHGLQVKQGKSALWSHLLHYQESKEKRDATPAGLGPPGIIHDTERRKSDASCAMPPLKVSLIQDMRHIQNISEIKTDVGKARAWVRLSMEKKLLSRHLKQLLSDHELTKKLYKRYAFLRCDDEKEQFLYHLLSFNAVDYFCFTNVFTTIMIPYHVVIIPSKKLGGSMFTANPWVCVSGELAETGVLQVPKNTLEITFECQNLGKLTTVQMGHDNSGLYAKWLVECVMVRNGITGHTYKFPCGRWLGKGVDDGSLERILVGELVTNMESDERMCRTPPMQQSPGMMRRFVTISPNSKPKLNTGQIQESVGEAINGIVKHFHKPEKERGSLTLLLCGEYGLVWALEQVFQHGFKSPRLFKNVFIWDFLEKAQVYFESAERSQMTQDENWQMRVRHFCHFMRAINSTPRNIGKDGKFQMLVCLGARDHLLHHWIALLAECPITAQMYEDVALLKDHSLVNSLIRVLQTLQEFNITLEASLVKGIGI

Target ORF information:

RefSeq Version	XM_005166611.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio DENN/MADD domain containing 5A (dennd5a), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005166611.4

ORF Insert Sequence:

ATGAGCACCG GATTCAGCTC CAGCTCCTGC CGCTTCGCGG ATTATTTCGT GATCTGCGGT 
CTGGACACCG AGAGCGGCTT AGAACCAGAC GAGCTGTCCG GTGAGAATTT TGAGCAGAGT 
CCACTGCGGA GAACGTTTAA ATCGAAGGTT CTAGCGCATT ATCCTGACAA TGTGGAGTGG 
AACCCTTTTG ACCAGGATGC AGTGGGAATG CTCTGTATGC CAAAAGGCCT GTCCTTCCGC 
ACTCAAACAG ACACCCGTGA GCCCCAGTTC CACTCCTTCA TTATCACCAG AGAGGACGGC 
TCCCGGACGT ACGGCTTTGC CCTCACTTTC TTTGAGGAGG TGACCAGCAA ACAGATCTGC 
AGTGCCATGC AGACACTTTA TCACATGCAC AATGCGGAAC AGTATGATAT CCTGCACACA 
CCACCCACGC CTCGCAGTCC AGAAGATCCC CGTCCCACCA CGCAACAACT GCACGCCTCC 
CCTGCTCTGT CCAGGCTGCA GCGCTTCAAC TCATACGACA TCAGCCGGGA CACGCTGTAC 
GTGTCCAAAT GCATCTGCCT GATCACCCCC ATGGCCTTCG CCCACGCCTG CAGGAAGGTT 
CTGGAGCAGC TGCATCAGGC CGTCACCTCA CCTCAGCCCC CGCCGCTGCC GCTGGAGAGC 
TATGTCTACA ATGTCCTCTA TGAGGTCCCA CTGCCACCAG CCGGACGCTC CTTGAAGTTT 
TCAGGCGTCT ACAGGCCAGT GATCTGTCAG AGACCCAGCA CAGCCGAGCT GCCCCTCTTT 
GACTTCCCCC TTCAGGAGGT GTTTGAGCTG CTAGGGCTGG AGAACATCTT GCAGCTCTTC 
ACCTGTGCGC TGCTGGAGAT CCAGATCCTT CTGTACTCAC AACATTACCA GCGGCTGATG 
ACGGTGGCGG AGAGCATCAC AGCCTTAATG TTCCCCTTTC AGTGGCAGCA TGTGTACGTG 
CCCATCCTGC CCGCTTCCCT GCTCCACTTC CTGGACGCTC CTGTGCCGTA CCTCATGGGT 
CTGCACTCTA ACGGCCAGGA TGACCGCACC AAACTGGAGC TTCCCCAAGA GGCCAATCTC 
TGCTTTGTCG ATATTGACAA CCACTTCATC GAGCTACCCG AAGACCTGCC TCAGTTCCCC 
AACAAGCTGG AGTTTATCCA GGAGATCTCT GAGGTTCTGC TGGCGTTTGG CTTGTCTCCA 
GAAGCGAACC CACAATGCAG CGAGGGCCAG AGCAAGATCA GGGGCTTCCG GTGTGGCGAC 
ATGGCCTCCG ACAAGCGCAA CGGGAACCTG GCCGGCTCGC CGCTCAATTC GTTCCTGCTG 
AGAGAAAACC AAACCATCGC TCGCCTGCAA GCGCTGGTCA AGAGGACTGG AGTCAGTCTG 
GAGAAGTTGG ACGTGAAGGA CGACGCAAGC ACTAATAAGG ATACGAAAGT GCAGTGTGAA 
GAAGAAGAGA TGAAAATGCA CCAGCTGAAC ATTCGGGTGC GAGAGGTCTT CGCCAACCGT 
TTCTCTCAGA TGTTCTCGGA TTATGAGGTG TTTGTTATTC AGCCCAGCCA AGACAAAGAG 
TCCTGGTTCA GCAACCGAGA CCAGATGCAG AACTTTGACA AGGCCTCCTT CCTGTCAGAT 
CAGCCCGAAC CGTACCTGCC GTTCCTCTCC CGCTTTCTGG AGACGCAGAT GTTTGCCTCT 
TTTATCGACA GTAAGATTCT GTGCCACGAT GACGAGGACA AAGAGCACAC ACTGCGAGTC 
TTTGATTCCC GTGTTGACAA GATCCGCATG ATAAATGTCA GGACACCCAC CCTGCGGACC 
TCCATGTACC AGAAATGCAC CAATATCGAT GAAGCAGAAA AAGCCATAGA GGTGAGACTA 
TGTAAGATCG ACCACACGGC TCTGCATCCC CATCTGCTGG ACATGAAGAT CGGTCAGGGC 
CGCTATGAGC CAGGCTTTTT CCCTCGCTTA CAGTCTGATG TGCTCTCCAC TGGACCCACC 
AGCAACAAGT GGGCGAAGCG CAGTGCACCG GCCCAATGGC GGAGGAAAGA CCGACAGAAA 
CAGCACGCCG AACATCTCTA TCTGGACAAT GACCAGAGAG AGAAATACAT CCAGGAAGCC 
AGAAACCTTG GCACCACACT CCGTCAGCCA AAACTCTCCA ACCTGTCGCC TTCGGTCATT 
GCACAGACCA ACTGGAAGTT TGTTGAGGGA CTGTTGAAGG AGTGCAGAAA TAAGACAAAG 
CGCATGCTGG TGGAGAAGAT GGGACGGGAG GCAGTGGAGC TGGGCCACGG TGAGGTGAGC 
ATCACCGGTG TGGAGGAAAA CACACTCATC GCCAGCCTGT GTGACCTGCT GGAGCGAATC 
TGGAGCCACG GCCTACAGGT CAAACAGGGA AAATCCGCCC TGTGGTCGCA CCTTTTACAT 
TATCAAGAGA GTAAAGAGAA GAGAGACGCT ACTCCAGCAG GCCTCGGCCC TCCTGGTATC 
ATTCATGACA CAGAGAGACG GAAGTCTGAT GCTAGCTGTG CGATGCCGCC TCTCAAAGTC 
TCCTTGATTC AGGACATGAG GCACATCCAG AACATCAGCG AGATCAAGAC GGACGTTGGC 
AAGGCCAGGG CTTGGGTGCG TCTCTCCATG GAGAAGAAAC TGCTGTCCAG ACACCTGAAG 
CAACTGCTGT CTGACCACGA GCTCACCAAG AAACTCTATA AGCGATATGC TTTTCTGCGC 
TGTGATGACG AGAAGGAGCA GTTCCTCTAC CATCTCCTCT CTTTCAACGC TGTGGACTAC 
TTCTGCTTCA CTAATGTCTT CACCACAATT ATGATTCCAT ACCATGTGGT CATCATTCCT 
AGTAAGAAAC TCGGTGGCTC TATGTTCACA GCCAACCCCT GGGTGTGTGT CTCAGGGGAA 
CTAGCCGAGA CTGGGGTCCT GCAGGTCCCT AAAAACACAC TGGAGATTAC ATTTGAGTGC 
CAGAATCTGG GGAAGCTGAC CACTGTACAG ATGGGCCATG ATAACTCAGG GCTGTATGCT 
AAATGGCTAG TGGAGTGCGT AATGGTCCGC AATGGGATTA CAGGACACAC CTATAAGTTT 
CCATGTGGCC GTTGGCTGGG TAAAGGAGTA GATGATGGCA GCTTGGAGAG GATCCTGGTG 
GGCGAATTGG TGACTAACAT GGAGAGCGAT GAAAGAATGT GCCGGACTCC TCCGATGCAG 
CAATCCCCCG GGATGATGCG AAGATTTGTC ACCATTTCGC CAAACAGCAA ACCAAAGTTA 
AACACGGGCC AGATCCAGGA AAGTGTGGGA GAGGCGATTA ACGGGATCGT CAAACACTTC 
CATAAGCCGG AGAAAGAGAG AGGAAGCCTC ACGTTGCTTC TGTGTGGGGA GTATGGTTTG 
GTTTGGGCTC TGGAGCAGGT GTTTCAGCAT GGCTTCAAGT CACCGCGACT GTTCAAGAAC 
GTCTTCATTT GGGACTTTCT AGAAAAGGCG CAGGTTTACT TTGAGAGTGC GGAGCGGAGT 
CAGATGACCC AGGATGAGAA CTGGCAGATG AGGGTCCGTC ATTTCTGCCA TTTCATGCGC 
GCCATCAACA GCACACCCAG AAACATCGGC AAAGATGGGA AGTTTCAGAT GCTTGTGTGT 
CTTGGGGCCA GAGATCACCT GCTCCACCAT TGGATCGCGC TTCTTGCCGA GTGTCCAATC 
ACAGCACAGA TGTACGAGGA CGTGGCGCTC TTAAAGGACC ATTCATTGGT GAACTCTCTG 
ATTCGAGTAC TACAGACTCT GCAGGAGTTC AATATCACTC TGGAAGCTTC TCTCGTCAAA 
GGGATTGGGA TATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dennd5a ( XM_009303571.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009301846.1 Danio rerio DENN/MADD domain containing 5A (dennd5a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa47407
Clone ID Related Accession (Same CDS sequence)	XM_009303571.3 , XM_009303571.2
Accession Version	XM_009303571.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3972bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	DENN domain-containing protein 5A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009303571.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCACCG GATTCAGCTC CAGCTCCTGC CGCTTCGCGG ATTATTTCGT GATCTGCGGT 
CTGGACACCG AGAGCGGCTT AGAACCAGAC GAGCTGTCCG CTTTATGCCA GTATATTCAG 
GCTACTAAAT TCACAGATGG CGCCAGAGGA AAGTTGGCGA GTGCAAATGA AGGTGAGAAT 
TTTGAGCAGA GTCCACTGCG GAGAACGTTT AAATCGAAGG TTCTAGCGCA TTATCCTGAC 
AATGTGGAGT GGAACCCTTT TGACCAGGAT GCAGTGGGAA TGCTCTGTAT GCCAAAAGGC 
CTGTCCTTCC GCACTCAAAC AGACACCCGT GAGCCCCAGT TCCACTCCTT CATTATCACC 
AGAGAGGACG GCTCCCGGAC GTACGGCTTT GCCCTCACTT TCTTTGAGGA GGTGACCAGC 
AAACAGATCT GCAGTGCCAT GCAGACACTT TATCACATGC ACAATGCGGA ACAGTATGAT 
ATCCTGCACA CACCACCCAC GCCTCGCAGT CCAGAAGATC CCCGTCCCAC CACGCAACAA 
CTGCACGCCT CCCCTGCTCT GTCCAGGCTG CAGCGCTTCA ACTCATACGA CATCAGCCGG 
GACACGCTGT ACGTGTCCAA ATGCATCTGC CTGATCACCC CCATGGCCTT CGCCCACGCC 
TGCAGGAAGG TTCTGGAGCA GCTGCATCAG GCCGTCACCT CACCTCAGCC CCCGCCGCTG 
CCGCTGGAGA GCTATGTCTA CAATGTCCTC TATGAGGTCC CACTGCCACC AGCCGGACGC 
TCCTTGAAGT TTTCAGGCGT CTACAGGCCA GTGATCTGTC AGAGACCCAG CACAGCCGAG 
CTGCCCCTCT TTGACTTCCC CCTTCAGGAG GTGTTTGAGC TGCTAGGGCT GGAGAACATC 
TTGCAGCTCT TCACCTGTGC GCTGCTGGAG ATCCAGATCC TTCTGTACTC ACAACATTAC 
CAGCGGCTGA TGACGGTGGC GGAGAGCATC ACAGCCTTAA TGTTCCCCTT TCAGTGGCAG 
CATGTGTACG TGCCCATCCT GCCCGCTTCC CTGCTCCACT TCCTGGACGC TCCTGTGCCG 
TACCTCATGG GTCTGCACTC TAACGGCCAG GATGACCGCA CCAAACTGGA GCTTCCCCAA 
GAGGCCAATC TCTGCTTTGT CGATATTGAC AACCACTTCA TCGAGCTACC CGAAGACCTG 
CCTCAGTTCC CCAACAAGCT GGAGTTTATC CAGGAGATCT CTGAGGTTCT GCTGGCGTTT 
GGCTTGTCTC CAGAAGCGAA CCCACAATGC AGCGAGGGCC AGAGCAAGAT CAGGGGCTTC 
CGGTGTGGCG ACATGGCCTC CGACAAGCGC AACGGGAACC TGGCCGGCTC GCCGCTCAAT 
TCGTTCCTGC TGAGAGAAAA CCAAACCATC GCTCGCCTGC AAGCGCTGGT CAAGAGGACT 
GGAGTCAGTC TGGAGAAGTT GGACGTGAAG GACGACGCAA GCACTAATAA GGATACGAAA 
GTGCAGTGTG AAGAAGAAGA GATGAAAATG CACCAGCTGA ACATTCGGGT GCGAGAGGTC 
TTCGCCAACC GTTTCTCTCA GATGTTCTCG GATTATGAGG TGTTTGTTAT TCAGCCCAGC 
CAAGACAAAG AGTCCTGGTT CAGCAACCGA GACCAGATGC AGAACTTTGA CAAGGCCTCC 
TTCCTGTCAG ATCAGCCCGA ACCGTACCTG CCGTTCCTCT CCCGCTTTCT GGAGACGCAG 
ATGTTTGCCT CTTTTATCGA CAGTAAGATT CTGTGCCACG ATGACGAGGA CAAAGAGCAC 
ACACTGCGAG TCTTTGATTC CCGTGTTGAC AAGATCCGCA TGATAAATGT CAGGACACCC 
ACCCTGCGGA CCTCCATGTA CCAGAAATGC ACCAATATCG ATGAAGCAGA AAAAGCCATA 
GAGGTGAGAC TATGTAAGAT CGACCACACG GCTCTGCATC CCCATCTGCT GGACATGAAG 
ATCGGTCAGG GCCGCTATGA GCCAGGCTTT TTCCCTCGCT TACAGTCTGA TGTGCTCTCC 
ACTGGACCCA CCAGCAACAA GTGGGCGAAG CGCAGTGCAC CGGCCCAATG GCGGAGGAAA 
GACCGACAGA AACAGCACGC CGAACATCTC TATCTGGACA ATGACCAGAG AGAGCATGTG 
GAGTGTCTGT TTTCTGAGCT TCAGCTTCTG CTGGCTCTGG ACTACTACTG GTTTTCGCAG 
TCCTTCAGGC CCTGTCTGAA GTCCGTCAAG GTGGATGTGA AATACATCCA GGAAGCCAGA 
AACCTTGGCA CCACACTCCG TCAGCCAAAA CTCTCCAACC TGTCGCCTTC GGTCATTGCA 
CAGACCAACT GGAAGTTTGT TGAGGGACTG TTGAAGGAGT GCAGAAATAA GACAAAGCGC 
ATGCTGGTGG AGAAGATGGG ACGGGAGGCA GTGGAGCTGG GCCACGGTGA GGTGAGCATC 
ACCGGTGTGG AGGAAAACAC ACTCATCGCC AGCCTGTGTG ACCTGCTGGA GCGAATCTGG 
AGCCACGGCC TACAGGTCAA ACAGGGAAAA TCCGCCCTGT GGTCGCACCT TTTACATTAT 
CAAGAGAGTA AAGAGAAGAG AGACGCTACT CCAGCAGGCC TCGGCCCTCC TGGTATCATT 
CATGACACAG AGAGACGGAA GTCTGATGCT AGCTGTGCGA TGCCGCCTCT CAAAGTCTCC 
TTGATTCAGG ACATGAGGCA CATCCAGAAC ATCAGCGAGA TCAAGACGGA CGTTGGCAAG 
GCCAGGGCTT GGGTGCGTCT CTCCATGGAG AAGAAACTGC TGTCCAGACA CCTGAAGCAA 
CTGCTGTCTG ACCACGAGCT CACCAAGAAA CTCTATAAGC GATATGCTTT TCTGCGCTGT 
GATGACGAGA AGGAGCAGTT CCTCTACCAT CTCCTCTCTT TCAACGCTGT GGACTACTTC 
TGCTTCACTA ATGTCTTCAC CACAATTATG ATTCCATACC ATGTGGTCAT CATTCCTAGT 
AAGAAACTCG GTGGCTCTAT GTTCACAGCC AACCCCTGGG TGTGTGTCTC AGGGGAACTA 
GCCGAGACTG GGGTCCTGCA GGTCCCTAAA AACACACTGG AGATTACATT TGAGTGCCAG 
AATCTGGGGA AGCTGACCAC TGTACAGATG GGCCATGATA ACTCAGGGCT GTATGCTAAA 
TGGCTAGTGG AGTGCGTAAT GGTCCGCAAT GGGATTACAG GACACACCTA TAAGTTTCCA 
TGTGGCCGTT GGCTGGGTAA AGGAGTAGAT GATGGCAGCT TGGAGAGGAT CCTGGTGGGC 
GAATTGGTGA CTAACATGGA GAGCGATGAA AGAATGTGCC GGACTCCTCC GATGCAGCAA 
TCCCCCGGGA TGATGCGAAG ATTTGTCACC ATTTCGCCAA ACAGCAAACC AAAGTTAAAC 
ACGGGCCAGA TCCAGGAAAG TGTGGGAGAG GCGATTAACG GGATCGTCAA ACACTTCCAT 
AAGCCGGAGA AAGAGAGAGG AAGCCTCACG TTGCTTCTGT GTGGGGAGTA TGGTTTGGTT 
TGGGCTCTGG AGCAGGTGTT TCAGCATGGC TTCAAGTCAC CGCGACTGTT CAAGAACGTC 
TTCATTTGGG ACTTTCTAGA AAAGGCGCAG GTTTACTTTG AGAGTGCGGA GCGGAGTCAG 
ATGACCCAGG ATGAGAACTG GCAGATGAGG GTCCGTCATT TCTGCCATTT CATGCGCGCC 
ATCAACAGCA CACCCAGAAA CATCGGCAAA GATGGGAAGT TTCAGATGCT TGTGTGTCTT 
GGGGCCAGAG ATCACCTGCT CCACCATTGG ATCGCGCTTC TTGCCGAGTG TCCAATCACA 
GCACAGATGT ACGAGGACGT GGCGCTCTTA AAGGACCATT CATTGGTGAA CTCTCTGATT 
CGAGTACTAC AGACTCTGCA GGAGTTCAAT ATCACTCTGG AAGCTTCTCT CGTCAAAGGG 
ATTGGGATAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009301846.1
CDS	210..4181
Translation	MSTGFSSSSCRFADYFVICGLDTESGLEPDELSALCQYIQATKFTDGARGKLASANEGENFEQSPLRRTFKSKVLAHYPDNVEWNPFDQDAVGMLCMPKGLSFRTQTDTREPQFHSFIITREDGSRTYGFALTFFEEVTSKQICSAMQTLYHMHNAEQYDILHTPPTPRSPEDPRPTTQQLHASPALSRLQRFNSYDISRDTLYVSKCICLITPMAFAHACRKVLEQLHQAVTSPQPPPLPLESYVYNVLYEVPLPPAGRSLKFSGVYRPVICQRPSTAELPLFDFPLQEVFELLGLENILQLFTCALLEIQILLYSQHYQRLMTVAESITALMFPFQWQHVYVPILPASLLHFLDAPVPYLMGLHSNGQDDRTKLELPQEANLCFVDIDNHFIELPEDLPQFPNKLEFIQEISEVLLAFGLSPEANPQCSEGQSKIRGFRCGDMASDKRNGNLAGSPLNSFLLRENQTIARLQALVKRTGVSLEKLDVKDDASTNKDTKVQCEEEEMKMHQLNIRVREVFANRFSQMFSDYEVFVIQPSQDKESWFSNRDQMQNFDKASFLSDQPEPYLPFLSRFLETQMFASFIDSKILCHDDEDKEHTLRVFDSRVDKIRMINVRTPTLRTSMYQKCTNIDEAEKAIEVRLCKIDHTALHPHLLDMKIGQGRYEPGFFPRLQSDVLSTGPTSNKWAKRSAPAQWRRKDRQKQHAEHLYLDNDQREHVECLFSELQLLLALDYYWFSQSFRPCLKSVKVDVKYIQEARNLGTTLRQPKLSNLSPSVIAQTNWKFVEGLLKECRNKTKRMLVEKMGREAVELGHGEVSITGVEENTLIASLCDLLERIWSHGLQVKQGKSALWSHLLHYQESKEKRDATPAGLGPPGIIHDTERRKSDASCAMPPLKVSLIQDMRHIQNISEIKTDVGKARAWVRLSMEKKLLSRHLKQLLSDHELTKKLYKRYAFLRCDDEKEQFLYHLLSFNAVDYFCFTNVFTTIMIPYHVVIIPSKKLGGSMFTANPWVCVSGELAETGVLQVPKNTLEITFECQNLGKLTTVQMGHDNSGLYAKWLVECVMVRNGITGHTYKFPCGRWLGKGVDDGSLERILVGELVTNMESDERMCRTPPMQQSPGMMRRFVTISPNSKPKLNTGQIQESVGEAINGIVKHFHKPEKERGSLTLLLCGEYGLVWALEQVFQHGFKSPRLFKNVFIWDFLEKAQVYFESAERSQMTQDENWQMRVRHFCHFMRAINSTPRNIGKDGKFQMLVCLGARDHLLHHWIALLAECPITAQMYEDVALLKDHSLVNSLIRVLQTLQEFNITLEASLVKGIGI

Target ORF information:

RefSeq Version	XM_009303571.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio DENN/MADD domain containing 5A (dennd5a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009303571.3

ORF Insert Sequence:

ATGAGCACCG GATTCAGCTC CAGCTCCTGC CGCTTCGCGG ATTATTTCGT GATCTGCGGT 
CTGGACACCG AGAGCGGCTT AGAACCAGAC GAGCTGTCCG CTTTATGCCA GTATATTCAG 
GCTACTAAAT TCACAGATGG CGCCAGAGGA AAGTTGGCGA GTGCAAATGA AGGTGAGAAT 
TTTGAGCAGA GTCCACTGCG GAGAACGTTT AAATCGAAGG TTCTAGCGCA TTATCCTGAC 
AATGTGGAGT GGAACCCTTT TGACCAGGAT GCAGTGGGAA TGCTCTGTAT GCCAAAAGGC 
CTGTCCTTCC GCACTCAAAC AGACACCCGT GAGCCCCAGT TCCACTCCTT CATTATCACC 
AGAGAGGACG GCTCCCGGAC GTACGGCTTT GCCCTCACTT TCTTTGAGGA GGTGACCAGC 
AAACAGATCT GCAGTGCCAT GCAGACACTT TATCACATGC ACAATGCGGA ACAGTATGAT 
ATCCTGCACA CACCACCCAC GCCTCGCAGT CCAGAAGATC CCCGTCCCAC CACGCAACAA 
CTGCACGCCT CCCCTGCTCT GTCCAGGCTG CAGCGCTTCA ACTCATACGA CATCAGCCGG 
GACACGCTGT ACGTGTCCAA ATGCATCTGC CTGATCACCC CCATGGCCTT CGCCCACGCC 
TGCAGGAAGG TTCTGGAGCA GCTGCATCAG GCCGTCACCT CACCTCAGCC CCCGCCGCTG 
CCGCTGGAGA GCTATGTCTA CAATGTCCTC TATGAGGTCC CACTGCCACC AGCCGGACGC 
TCCTTGAAGT TTTCAGGCGT CTACAGGCCA GTGATCTGTC AGAGACCCAG CACAGCCGAG 
CTGCCCCTCT TTGACTTCCC CCTTCAGGAG GTGTTTGAGC TGCTAGGGCT GGAGAACATC 
TTGCAGCTCT TCACCTGTGC GCTGCTGGAG ATCCAGATCC TTCTGTACTC ACAACATTAC 
CAGCGGCTGA TGACGGTGGC GGAGAGCATC ACAGCCTTAA TGTTCCCCTT TCAGTGGCAG 
CATGTGTACG TGCCCATCCT GCCCGCTTCC CTGCTCCACT TCCTGGACGC TCCTGTGCCG 
TACCTCATGG GTCTGCACTC TAACGGCCAG GATGACCGCA CCAAACTGGA GCTTCCCCAA 
GAGGCCAATC TCTGCTTTGT CGATATTGAC AACCACTTCA TCGAGCTACC CGAAGACCTG 
CCTCAGTTCC CCAACAAGCT GGAGTTTATC CAGGAGATCT CTGAGGTTCT GCTGGCGTTT 
GGCTTGTCTC CAGAAGCGAA CCCACAATGC AGCGAGGGCC AGAGCAAGAT CAGGGGCTTC 
CGGTGTGGCG ACATGGCCTC CGACAAGCGC AACGGGAACC TGGCCGGCTC GCCGCTCAAT 
TCGTTCCTGC TGAGAGAAAA CCAAACCATC GCTCGCCTGC AAGCGCTGGT CAAGAGGACT 
GGAGTCAGTC TGGAGAAGTT GGACGTGAAG GACGACGCAA GCACTAATAA GGATACGAAA 
GTGCAGTGTG AAGAAGAAGA GATGAAAATG CACCAGCTGA ACATTCGGGT GCGAGAGGTC 
TTCGCCAACC GTTTCTCTCA GATGTTCTCG GATTATGAGG TGTTTGTTAT TCAGCCCAGC 
CAAGACAAAG AGTCCTGGTT CAGCAACCGA GACCAGATGC AGAACTTTGA CAAGGCCTCC 
TTCCTGTCAG ATCAGCCCGA ACCGTACCTG CCGTTCCTCT CCCGCTTTCT GGAGACGCAG 
ATGTTTGCCT CTTTTATCGA CAGTAAGATT CTGTGCCACG ATGACGAGGA CAAAGAGCAC 
ACACTGCGAG TCTTTGATTC CCGTGTTGAC AAGATCCGCA TGATAAATGT CAGGACACCC 
ACCCTGCGGA CCTCCATGTA CCAGAAATGC ACCAATATCG ATGAAGCAGA AAAAGCCATA 
GAGGTGAGAC TATGTAAGAT CGACCACACG GCTCTGCATC CCCATCTGCT GGACATGAAG 
ATCGGTCAGG GCCGCTATGA GCCAGGCTTT TTCCCTCGCT TACAGTCTGA TGTGCTCTCC 
ACTGGACCCA CCAGCAACAA GTGGGCGAAG CGCAGTGCAC CGGCCCAATG GCGGAGGAAA 
GACCGACAGA AACAGCACGC CGAACATCTC TATCTGGACA ATGACCAGAG AGAGCATGTG 
GAGTGTCTGT TTTCTGAGCT TCAGCTTCTG CTGGCTCTGG ACTACTACTG GTTTTCGCAG 
TCCTTCAGGC CCTGTCTGAA GTCCGTCAAG GTGGATGTGA AATACATCCA GGAAGCCAGA 
AACCTTGGCA CCACACTCCG TCAGCCAAAA CTCTCCAACC TGTCGCCTTC GGTCATTGCA 
CAGACCAACT GGAAGTTTGT TGAGGGACTG TTGAAGGAGT GCAGAAATAA GACAAAGCGC 
ATGCTGGTGG AGAAGATGGG ACGGGAGGCA GTGGAGCTGG GCCACGGTGA GGTGAGCATC 
ACCGGTGTGG AGGAAAACAC ACTCATCGCC AGCCTGTGTG ACCTGCTGGA GCGAATCTGG 
AGCCACGGCC TACAGGTCAA ACAGGGAAAA TCCGCCCTGT GGTCGCACCT TTTACATTAT 
CAAGAGAGTA AAGAGAAGAG AGACGCTACT CCAGCAGGCC TCGGCCCTCC TGGTATCATT 
CATGACACAG AGAGACGGAA GTCTGATGCT AGCTGTGCGA TGCCGCCTCT CAAAGTCTCC 
TTGATTCAGG ACATGAGGCA CATCCAGAAC ATCAGCGAGA TCAAGACGGA CGTTGGCAAG 
GCCAGGGCTT GGGTGCGTCT CTCCATGGAG AAGAAACTGC TGTCCAGACA CCTGAAGCAA 
CTGCTGTCTG ACCACGAGCT CACCAAGAAA CTCTATAAGC GATATGCTTT TCTGCGCTGT 
GATGACGAGA AGGAGCAGTT CCTCTACCAT CTCCTCTCTT TCAACGCTGT GGACTACTTC 
TGCTTCACTA ATGTCTTCAC CACAATTATG ATTCCATACC ATGTGGTCAT CATTCCTAGT 
AAGAAACTCG GTGGCTCTAT GTTCACAGCC AACCCCTGGG TGTGTGTCTC AGGGGAACTA 
GCCGAGACTG GGGTCCTGCA GGTCCCTAAA AACACACTGG AGATTACATT TGAGTGCCAG 
AATCTGGGGA AGCTGACCAC TGTACAGATG GGCCATGATA ACTCAGGGCT GTATGCTAAA 
TGGCTAGTGG AGTGCGTAAT GGTCCGCAAT GGGATTACAG GACACACCTA TAAGTTTCCA 
TGTGGCCGTT GGCTGGGTAA AGGAGTAGAT GATGGCAGCT TGGAGAGGAT CCTGGTGGGC 
GAATTGGTGA CTAACATGGA GAGCGATGAA AGAATGTGCC GGACTCCTCC GATGCAGCAA 
TCCCCCGGGA TGATGCGAAG ATTTGTCACC ATTTCGCCAA ACAGCAAACC AAAGTTAAAC 
ACGGGCCAGA TCCAGGAAAG TGTGGGAGAG GCGATTAACG GGATCGTCAA ACACTTCCAT 
AAGCCGGAGA AAGAGAGAGG AAGCCTCACG TTGCTTCTGT GTGGGGAGTA TGGTTTGGTT 
TGGGCTCTGG AGCAGGTGTT TCAGCATGGC TTCAAGTCAC CGCGACTGTT CAAGAACGTC 
TTCATTTGGG ACTTTCTAGA AAAGGCGCAG GTTTACTTTG AGAGTGCGGA GCGGAGTCAG 
ATGACCCAGG ATGAGAACTG GCAGATGAGG GTCCGTCATT TCTGCCATTT CATGCGCGCC 
ATCAACAGCA CACCCAGAAA CATCGGCAAA GATGGGAAGT TTCAGATGCT TGTGTGTCTT 
GGGGCCAGAG ATCACCTGCT CCACCATTGG ATCGCGCTTC TTGCCGAGTG TCCAATCACA 
GCACAGATGT ACGAGGACGT GGCGCTCTTA AAGGACCATT CATTGGTGAA CTCTCTGATT 
CGAGTACTAC AGACTCTGCA GGAGTTCAAT ATCACTCTGG AAGCTTCTCT CGTCAAAGGG 
ATTGGGATAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dennd5a ( XM_009303571.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009301846.1 Danio rerio DENN/MADD domain containing 5A (dennd5a), transcript variant X1, mRNA.

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CloneID	ODa47407
Clone ID Related Accession (Same CDS sequence)	XM_009303571.3 , XM_009303571.2
Accession Version	XM_009303571.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3972bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	DENN domain-containing protein 5A isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009303571.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCACCG GATTCAGCTC CAGCTCCTGC CGCTTCGCGG ATTATTTCGT GATCTGCGGT 
CTGGACACCG AGAGCGGCTT AGAACCAGAC GAGCTGTCCG CTTTATGCCA GTATATTCAG 
GCTACTAAAT TCACAGATGG CGCCAGAGGA AAGTTGGCGA GTGCAAATGA AGGTGAGAAT 
TTTGAGCAGA GTCCACTGCG GAGAACGTTT AAATCGAAGG TTCTAGCGCA TTATCCTGAC 
AATGTGGAGT GGAACCCTTT TGACCAGGAT GCAGTGGGAA TGCTCTGTAT GCCAAAAGGC 
CTGTCCTTCC GCACTCAAAC AGACACCCGT GAGCCCCAGT TCCACTCCTT CATTATCACC 
AGAGAGGACG GCTCCCGGAC GTACGGCTTT GCCCTCACTT TCTTTGAGGA GGTGACCAGC 
AAACAGATCT GCAGTGCCAT GCAGACACTT TATCACATGC ACAATGCGGA ACAGTATGAT 
ATCCTGCACA CACCACCCAC GCCTCGCAGT CCAGAAGATC CCCGTCCCAC CACGCAACAA 
CTGCACGCCT CCCCTGCTCT GTCCAGGCTG CAGCGCTTCA ACTCATACGA CATCAGCCGG 
GACACGCTGT ACGTGTCCAA ATGCATCTGC CTGATCACCC CCATGGCCTT CGCCCACGCC 
TGCAGGAAGG TTCTGGAGCA GCTGCATCAG GCCGTCACCT CACCTCAGCC CCCGCCGCTG 
CCGCTGGAGA GCTATGTCTA CAATGTCCTC TATGAGGTCC CACTGCCACC AGCCGGACGC 
TCCTTGAAGT TTTCAGGCGT CTACAGGCCA GTGATCTGTC AGAGACCCAG CACAGCCGAG 
CTGCCCCTCT TTGACTTCCC CCTTCAGGAG GTGTTTGAGC TGCTAGGGCT GGAGAACATC 
TTGCAGCTCT TCACCTGTGC GCTGCTGGAG ATCCAGATCC TTCTGTACTC ACAACATTAC 
CAGCGGCTGA TGACGGTGGC GGAGAGCATC ACAGCCTTAA TGTTCCCCTT TCAGTGGCAG 
CATGTGTACG TGCCCATCCT GCCCGCTTCC CTGCTCCACT TCCTGGACGC TCCTGTGCCG 
TACCTCATGG GTCTGCACTC TAACGGCCAG GATGACCGCA CCAAACTGGA GCTTCCCCAA 
GAGGCCAATC TCTGCTTTGT CGATATTGAC AACCACTTCA TCGAGCTACC CGAAGACCTG 
CCTCAGTTCC CCAACAAGCT GGAGTTTATC CAGGAGATCT CTGAGGTTCT GCTGGCGTTT 
GGCTTGTCTC CAGAAGCGAA CCCACAATGC AGCGAGGGCC AGAGCAAGAT CAGGGGCTTC 
CGGTGTGGCG ACATGGCCTC CGACAAGCGC AACGGGAACC TGGCCGGCTC GCCGCTCAAT 
TCGTTCCTGC TGAGAGAAAA CCAAACCATC GCTCGCCTGC AAGCGCTGGT CAAGAGGACT 
GGAGTCAGTC TGGAGAAGTT GGACGTGAAG GACGACGCAA GCACTAATAA GGATACGAAA 
GTGCAGTGTG AAGAAGAAGA GATGAAAATG CACCAGCTGA ACATTCGGGT GCGAGAGGTC 
TTCGCCAACC GTTTCTCTCA GATGTTCTCG GATTATGAGG TGTTTGTTAT TCAGCCCAGC 
CAAGACAAAG AGTCCTGGTT CAGCAACCGA GACCAGATGC AGAACTTTGA CAAGGCCTCC 
TTCCTGTCAG ATCAGCCCGA ACCGTACCTG CCGTTCCTCT CCCGCTTTCT GGAGACGCAG 
ATGTTTGCCT CTTTTATCGA CAGTAAGATT CTGTGCCACG ATGACGAGGA CAAAGAGCAC 
ACACTGCGAG TCTTTGATTC CCGTGTTGAC AAGATCCGCA TGATAAATGT CAGGACACCC 
ACCCTGCGGA CCTCCATGTA CCAGAAATGC ACCAATATCG ATGAAGCAGA AAAAGCCATA 
GAGGTGAGAC TATGTAAGAT CGACCACACG GCTCTGCATC CCCATCTGCT GGACATGAAG 
ATCGGTCAGG GCCGCTATGA GCCAGGCTTT TTCCCTCGCT TACAGTCTGA TGTGCTCTCC 
ACTGGACCCA CCAGCAACAA GTGGGCGAAG CGCAGTGCAC CGGCCCAATG GCGGAGGAAA 
GACCGACAGA AACAGCACGC CGAACATCTC TATCTGGACA ATGACCAGAG AGAGCATGTG 
GAGTGTCTGT TTTCTGAGCT TCAGCTTCTG CTGGCTCTGG ACTACTACTG GTTTTCGCAG 
TCCTTCAGGC CCTGTCTGAA GTCCGTCAAG GTGGATGTGA AATACATCCA GGAAGCCAGA 
AACCTTGGCA CCACACTCCG TCAGCCAAAA CTCTCCAACC TGTCGCCTTC GGTCATTGCA 
CAGACCAACT GGAAGTTTGT TGAGGGACTG TTGAAGGAGT GCAGAAATAA GACAAAGCGC 
ATGCTGGTGG AGAAGATGGG ACGGGAGGCA GTGGAGCTGG GCCACGGTGA GGTGAGCATC 
ACCGGTGTGG AGGAAAACAC ACTCATCGCC AGCCTGTGTG ACCTGCTGGA GCGAATCTGG 
AGCCACGGCC TACAGGTCAA ACAGGGAAAA TCCGCCCTGT GGTCGCACCT TTTACATTAT 
CAAGAGAGTA AAGAGAAGAG AGACGCTACT CCAGCAGGCC TCGGCCCTCC TGGTATCATT 
CATGACACAG AGAGACGGAA GTCTGATGCT AGCTGTGCGA TGCCGCCTCT CAAAGTCTCC 
TTGATTCAGG ACATGAGGCA CATCCAGAAC ATCAGCGAGA TCAAGACGGA CGTTGGCAAG 
GCCAGGGCTT GGGTGCGTCT CTCCATGGAG AAGAAACTGC TGTCCAGACA CCTGAAGCAA 
CTGCTGTCTG ACCACGAGCT CACCAAGAAA CTCTATAAGC GATATGCTTT TCTGCGCTGT 
GATGACGAGA AGGAGCAGTT CCTCTACCAT CTCCTCTCTT TCAACGCTGT GGACTACTTC 
TGCTTCACTA ATGTCTTCAC CACAATTATG ATTCCATACC ATGTGGTCAT CATTCCTAGT 
AAGAAACTCG GTGGCTCTAT GTTCACAGCC AACCCCTGGG TGTGTGTCTC AGGGGAACTA 
GCCGAGACTG GGGTCCTGCA GGTCCCTAAA AACACACTGG AGATTACATT TGAGTGCCAG 
AATCTGGGGA AGCTGACCAC TGTACAGATG GGCCATGATA ACTCAGGGCT GTATGCTAAA 
TGGCTAGTGG AGTGCGTAAT GGTCCGCAAT GGGATTACAG GACACACCTA TAAGTTTCCA 
TGTGGCCGTT GGCTGGGTAA AGGAGTAGAT GATGGCAGCT TGGAGAGGAT CCTGGTGGGC 
GAATTGGTGA CTAACATGGA GAGCGATGAA AGAATGTGCC GGACTCCTCC GATGCAGCAA 
TCCCCCGGGA TGATGCGAAG ATTTGTCACC ATTTCGCCAA ACAGCAAACC AAAGTTAAAC 
ACGGGCCAGA TCCAGGAAAG TGTGGGAGAG GCGATTAACG GGATCGTCAA ACACTTCCAT 
AAGCCGGAGA AAGAGAGAGG AAGCCTCACG TTGCTTCTGT GTGGGGAGTA TGGTTTGGTT 
TGGGCTCTGG AGCAGGTGTT TCAGCATGGC TTCAAGTCAC CGCGACTGTT CAAGAACGTC 
TTCATTTGGG ACTTTCTAGA AAAGGCGCAG GTTTACTTTG AGAGTGCGGA GCGGAGTCAG 
ATGACCCAGG ATGAGAACTG GCAGATGAGG GTCCGTCATT TCTGCCATTT CATGCGCGCC 
ATCAACAGCA CACCCAGAAA CATCGGCAAA GATGGGAAGT TTCAGATGCT TGTGTGTCTT 
GGGGCCAGAG ATCACCTGCT CCACCATTGG ATCGCGCTTC TTGCCGAGTG TCCAATCACA 
GCACAGATGT ACGAGGACGT GGCGCTCTTA AAGGACCATT CATTGGTGAA CTCTCTGATT 
CGAGTACTAC AGACTCTGCA GGAGTTCAAT ATCACTCTGG AAGCTTCTCT CGTCAAAGGG 
ATTGGGATAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009301846.1
CDS	205..4176
Translation	MSTGFSSSSCRFADYFVICGLDTESGLEPDELSALCQYIQATKFTDGARGKLASANEGENFEQSPLRRTFKSKVLAHYPDNVEWNPFDQDAVGMLCMPKGLSFRTQTDTREPQFHSFIITREDGSRTYGFALTFFEEVTSKQICSAMQTLYHMHNAEQYDILHTPPTPRSPEDPRPTTQQLHASPALSRLQRFNSYDISRDTLYVSKCICLITPMAFAHACRKVLEQLHQAVTSPQPPPLPLESYVYNVLYEVPLPPAGRSLKFSGVYRPVICQRPSTAELPLFDFPLQEVFELLGLENILQLFTCALLEIQILLYSQHYQRLMTVAESITALMFPFQWQHVYVPILPASLLHFLDAPVPYLMGLHSNGQDDRTKLELPQEANLCFVDIDNHFIELPEDLPQFPNKLEFIQEISEVLLAFGLSPEANPQCSEGQSKIRGFRCGDMASDKRNGNLAGSPLNSFLLRENQTIARLQALVKRTGVSLEKLDVKDDASTNKDTKVQCEEEEMKMHQLNIRVREVFANRFSQMFSDYEVFVIQPSQDKESWFSNRDQMQNFDKASFLSDQPEPYLPFLSRFLETQMFASFIDSKILCHDDEDKEHTLRVFDSRVDKIRMINVRTPTLRTSMYQKCTNIDEAEKAIEVRLCKIDHTALHPHLLDMKIGQGRYEPGFFPRLQSDVLSTGPTSNKWAKRSAPAQWRRKDRQKQHAEHLYLDNDQREHVECLFSELQLLLALDYYWFSQSFRPCLKSVKVDVKYIQEARNLGTTLRQPKLSNLSPSVIAQTNWKFVEGLLKECRNKTKRMLVEKMGREAVELGHGEVSITGVEENTLIASLCDLLERIWSHGLQVKQGKSALWSHLLHYQESKEKRDATPAGLGPPGIIHDTERRKSDASCAMPPLKVSLIQDMRHIQNISEIKTDVGKARAWVRLSMEKKLLSRHLKQLLSDHELTKKLYKRYAFLRCDDEKEQFLYHLLSFNAVDYFCFTNVFTTIMIPYHVVIIPSKKLGGSMFTANPWVCVSGELAETGVLQVPKNTLEITFECQNLGKLTTVQMGHDNSGLYAKWLVECVMVRNGITGHTYKFPCGRWLGKGVDDGSLERILVGELVTNMESDERMCRTPPMQQSPGMMRRFVTISPNSKPKLNTGQIQESVGEAINGIVKHFHKPEKERGSLTLLLCGEYGLVWALEQVFQHGFKSPRLFKNVFIWDFLEKAQVYFESAERSQMTQDENWQMRVRHFCHFMRAINSTPRNIGKDGKFQMLVCLGARDHLLHHWIALLAECPITAQMYEDVALLKDHSLVNSLIRVLQTLQEFNITLEASLVKGIGI

Target ORF information:

RefSeq Version	XM_009303571.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio DENN/MADD domain containing 5A (dennd5a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009303571.2

ORF Insert Sequence:

ATGAGCACCG GATTCAGCTC CAGCTCCTGC CGCTTCGCGG ATTATTTCGT GATCTGCGGT 
CTGGACACCG AGAGCGGCTT AGAACCAGAC GAGCTGTCCG CTTTATGCCA GTATATTCAG 
GCTACTAAAT TCACAGATGG CGCCAGAGGA AAGTTGGCGA GTGCAAATGA AGGTGAGAAT 
TTTGAGCAGA GTCCACTGCG GAGAACGTTT AAATCGAAGG TTCTAGCGCA TTATCCTGAC 
AATGTGGAGT GGAACCCTTT TGACCAGGAT GCAGTGGGAA TGCTCTGTAT GCCAAAAGGC 
CTGTCCTTCC GCACTCAAAC AGACACCCGT GAGCCCCAGT TCCACTCCTT CATTATCACC 
AGAGAGGACG GCTCCCGGAC GTACGGCTTT GCCCTCACTT TCTTTGAGGA GGTGACCAGC 
AAACAGATCT GCAGTGCCAT GCAGACACTT TATCACATGC ACAATGCGGA ACAGTATGAT 
ATCCTGCACA CACCACCCAC GCCTCGCAGT CCAGAAGATC CCCGTCCCAC CACGCAACAA 
CTGCACGCCT CCCCTGCTCT GTCCAGGCTG CAGCGCTTCA ACTCATACGA CATCAGCCGG 
GACACGCTGT ACGTGTCCAA ATGCATCTGC CTGATCACCC CCATGGCCTT CGCCCACGCC 
TGCAGGAAGG TTCTGGAGCA GCTGCATCAG GCCGTCACCT CACCTCAGCC CCCGCCGCTG 
CCGCTGGAGA GCTATGTCTA CAATGTCCTC TATGAGGTCC CACTGCCACC AGCCGGACGC 
TCCTTGAAGT TTTCAGGCGT CTACAGGCCA GTGATCTGTC AGAGACCCAG CACAGCCGAG 
CTGCCCCTCT TTGACTTCCC CCTTCAGGAG GTGTTTGAGC TGCTAGGGCT GGAGAACATC 
TTGCAGCTCT TCACCTGTGC GCTGCTGGAG ATCCAGATCC TTCTGTACTC ACAACATTAC 
CAGCGGCTGA TGACGGTGGC GGAGAGCATC ACAGCCTTAA TGTTCCCCTT TCAGTGGCAG 
CATGTGTACG TGCCCATCCT GCCCGCTTCC CTGCTCCACT TCCTGGACGC TCCTGTGCCG 
TACCTCATGG GTCTGCACTC TAACGGCCAG GATGACCGCA CCAAACTGGA GCTTCCCCAA 
GAGGCCAATC TCTGCTTTGT CGATATTGAC AACCACTTCA TCGAGCTACC CGAAGACCTG 
CCTCAGTTCC CCAACAAGCT GGAGTTTATC CAGGAGATCT CTGAGGTTCT GCTGGCGTTT 
GGCTTGTCTC CAGAAGCGAA CCCACAATGC AGCGAGGGCC AGAGCAAGAT CAGGGGCTTC 
CGGTGTGGCG ACATGGCCTC CGACAAGCGC AACGGGAACC TGGCCGGCTC GCCGCTCAAT 
TCGTTCCTGC TGAGAGAAAA CCAAACCATC GCTCGCCTGC AAGCGCTGGT CAAGAGGACT 
GGAGTCAGTC TGGAGAAGTT GGACGTGAAG GACGACGCAA GCACTAATAA GGATACGAAA 
GTGCAGTGTG AAGAAGAAGA GATGAAAATG CACCAGCTGA ACATTCGGGT GCGAGAGGTC 
TTCGCCAACC GTTTCTCTCA GATGTTCTCG GATTATGAGG TGTTTGTTAT TCAGCCCAGC 
CAAGACAAAG AGTCCTGGTT CAGCAACCGA GACCAGATGC AGAACTTTGA CAAGGCCTCC 
TTCCTGTCAG ATCAGCCCGA ACCGTACCTG CCGTTCCTCT CCCGCTTTCT GGAGACGCAG 
ATGTTTGCCT CTTTTATCGA CAGTAAGATT CTGTGCCACG ATGACGAGGA CAAAGAGCAC 
ACACTGCGAG TCTTTGATTC CCGTGTTGAC AAGATCCGCA TGATAAATGT CAGGACACCC 
ACCCTGCGGA CCTCCATGTA CCAGAAATGC ACCAATATCG ATGAAGCAGA AAAAGCCATA 
GAGGTGAGAC TATGTAAGAT CGACCACACG GCTCTGCATC CCCATCTGCT GGACATGAAG 
ATCGGTCAGG GCCGCTATGA GCCAGGCTTT TTCCCTCGCT TACAGTCTGA TGTGCTCTCC 
ACTGGACCCA CCAGCAACAA GTGGGCGAAG CGCAGTGCAC CGGCCCAATG GCGGAGGAAA 
GACCGACAGA AACAGCACGC CGAACATCTC TATCTGGACA ATGACCAGAG AGAGCATGTG 
GAGTGTCTGT TTTCTGAGCT TCAGCTTCTG CTGGCTCTGG ACTACTACTG GTTTTCGCAG 
TCCTTCAGGC CCTGTCTGAA GTCCGTCAAG GTGGATGTGA AATACATCCA GGAAGCCAGA 
AACCTTGGCA CCACACTCCG TCAGCCAAAA CTCTCCAACC TGTCGCCTTC GGTCATTGCA 
CAGACCAACT GGAAGTTTGT TGAGGGACTG TTGAAGGAGT GCAGAAATAA GACAAAGCGC 
ATGCTGGTGG AGAAGATGGG ACGGGAGGCA GTGGAGCTGG GCCACGGTGA GGTGAGCATC 
ACCGGTGTGG AGGAAAACAC ACTCATCGCC AGCCTGTGTG ACCTGCTGGA GCGAATCTGG 
AGCCACGGCC TACAGGTCAA ACAGGGAAAA TCCGCCCTGT GGTCGCACCT TTTACATTAT 
CAAGAGAGTA AAGAGAAGAG AGACGCTACT CCAGCAGGCC TCGGCCCTCC TGGTATCATT 
CATGACACAG AGAGACGGAA GTCTGATGCT AGCTGTGCGA TGCCGCCTCT CAAAGTCTCC 
TTGATTCAGG ACATGAGGCA CATCCAGAAC ATCAGCGAGA TCAAGACGGA CGTTGGCAAG 
GCCAGGGCTT GGGTGCGTCT CTCCATGGAG AAGAAACTGC TGTCCAGACA CCTGAAGCAA 
CTGCTGTCTG ACCACGAGCT CACCAAGAAA CTCTATAAGC GATATGCTTT TCTGCGCTGT 
GATGACGAGA AGGAGCAGTT CCTCTACCAT CTCCTCTCTT TCAACGCTGT GGACTACTTC 
TGCTTCACTA ATGTCTTCAC CACAATTATG ATTCCATACC ATGTGGTCAT CATTCCTAGT 
AAGAAACTCG GTGGCTCTAT GTTCACAGCC AACCCCTGGG TGTGTGTCTC AGGGGAACTA 
GCCGAGACTG GGGTCCTGCA GGTCCCTAAA AACACACTGG AGATTACATT TGAGTGCCAG 
AATCTGGGGA AGCTGACCAC TGTACAGATG GGCCATGATA ACTCAGGGCT GTATGCTAAA 
TGGCTAGTGG AGTGCGTAAT GGTCCGCAAT GGGATTACAG GACACACCTA TAAGTTTCCA 
TGTGGCCGTT GGCTGGGTAA AGGAGTAGAT GATGGCAGCT TGGAGAGGAT CCTGGTGGGC 
GAATTGGTGA CTAACATGGA GAGCGATGAA AGAATGTGCC GGACTCCTCC GATGCAGCAA 
TCCCCCGGGA TGATGCGAAG ATTTGTCACC ATTTCGCCAA ACAGCAAACC AAAGTTAAAC 
ACGGGCCAGA TCCAGGAAAG TGTGGGAGAG GCGATTAACG GGATCGTCAA ACACTTCCAT 
AAGCCGGAGA AAGAGAGAGG AAGCCTCACG TTGCTTCTGT GTGGGGAGTA TGGTTTGGTT 
TGGGCTCTGG AGCAGGTGTT TCAGCATGGC TTCAAGTCAC CGCGACTGTT CAAGAACGTC 
TTCATTTGGG ACTTTCTAGA AAAGGCGCAG GTTTACTTTG AGAGTGCGGA GCGGAGTCAG 
ATGACCCAGG ATGAGAACTG GCAGATGAGG GTCCGTCATT TCTGCCATTT CATGCGCGCC 
ATCAACAGCA CACCCAGAAA CATCGGCAAA GATGGGAAGT TTCAGATGCT TGTGTGTCTT 
GGGGCCAGAG ATCACCTGCT CCACCATTGG ATCGCGCTTC TTGCCGAGTG TCCAATCACA 
GCACAGATGT ACGAGGACGT GGCGCTCTTA AAGGACCATT CATTGGTGAA CTCTCTGATT 
CGAGTACTAC AGACTCTGCA GGAGTTCAAT ATCACTCTGG AAGCTTCTCT CGTCAAAGGG 
ATTGGGATAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dennd5a ( XM_009303572.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009301847.1 Danio rerio DENN/MADD domain containing 5A (dennd5a), transcript variant X2, mRNA.

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CloneID	ODa47408
Clone ID Related Accession (Same CDS sequence)	XM_009303572.3 , XM_009303572.2
Accession Version	XM_009303572.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3900bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	DENN domain-containing protein 5A isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009303572.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCACCG GATTCAGCTC CAGCTCCTGC CGCTTCGCGG ATTATTTCGT GATCTGCGGT 
CTGGACACCG AGAGCGGCTT AGAACCAGAC GAGCTGTCCG GTGAGAATTT TGAGCAGAGT 
CCACTGCGGA GAACGTTTAA ATCGAAGGTT CTAGCGCATT ATCCTGACAA TGTGGAGTGG 
AACCCTTTTG ACCAGGATGC AGTGGGAATG CTCTGTATGC CAAAAGGCCT GTCCTTCCGC 
ACTCAAACAG ACACCCGTGA GCCCCAGTTC CACTCCTTCA TTATCACCAG AGAGGACGGC 
TCCCGGACGT ACGGCTTTGC CCTCACTTTC TTTGAGGAGG TGACCAGCAA ACAGATCTGC 
AGTGCCATGC AGACACTTTA TCACATGCAC AATGCGGAAC AGTATGATAT CCTGCACACA 
CCACCCACGC CTCGCAGTCC AGAAGATCCC CGTCCCACCA CGCAACAACT GCACGCCTCC 
CCTGCTCTGT CCAGGCTGCA GCGCTTCAAC TCATACGACA TCAGCCGGGA CACGCTGTAC 
GTGTCCAAAT GCATCTGCCT GATCACCCCC ATGGCCTTCG CCCACGCCTG CAGGAAGGTT 
CTGGAGCAGC TGCATCAGGC CGTCACCTCA CCTCAGCCCC CGCCGCTGCC GCTGGAGAGC 
TATGTCTACA ATGTCCTCTA TGAGGTCCCA CTGCCACCAG CCGGACGCTC CTTGAAGTTT 
TCAGGCGTCT ACAGGCCAGT GATCTGTCAG AGACCCAGCA CAGCCGAGCT GCCCCTCTTT 
GACTTCCCCC TTCAGGAGGT GTTTGAGCTG CTAGGGCTGG AGAACATCTT GCAGCTCTTC 
ACCTGTGCGC TGCTGGAGAT CCAGATCCTT CTGTACTCAC AACATTACCA GCGGCTGATG 
ACGGTGGCGG AGAGCATCAC AGCCTTAATG TTCCCCTTTC AGTGGCAGCA TGTGTACGTG 
CCCATCCTGC CCGCTTCCCT GCTCCACTTC CTGGACGCTC CTGTGCCGTA CCTCATGGGT 
CTGCACTCTA ACGGCCAGGA TGACCGCACC AAACTGGAGC TTCCCCAAGA GGCCAATCTC 
TGCTTTGTCG ATATTGACAA CCACTTCATC GAGCTACCCG AAGACCTGCC TCAGTTCCCC 
AACAAGCTGG AGTTTATCCA GGAGATCTCT GAGGTTCTGC TGGCGTTTGG CTTGTCTCCA 
GAAGCGAACC CACAATGCAG CGAGGGCCAG AGCAAGATCA GGGGCTTCCG GTGTGGCGAC 
ATGGCCTCCG ACAAGCGCAA CGGGAACCTG GCCGGCTCGC CGCTCAATTC GTTCCTGCTG 
AGAGAAAACC AAACCATCGC TCGCCTGCAA GCGCTGGTCA AGAGGACTGG AGTCAGTCTG 
GAGAAGTTGG ACGTGAAGGA CGACGCAAGC ACTAATAAGG ATACGAAAGT GCAGTGTGAA 
GAAGAAGAGA TGAAAATGCA CCAGCTGAAC ATTCGGGTGC GAGAGGTCTT CGCCAACCGT 
TTCTCTCAGA TGTTCTCGGA TTATGAGGTG TTTGTTATTC AGCCCAGCCA AGACAAAGAG 
TCCTGGTTCA GCAACCGAGA CCAGATGCAG AACTTTGACA AGGCCTCCTT CCTGTCAGAT 
CAGCCCGAAC CGTACCTGCC GTTCCTCTCC CGCTTTCTGG AGACGCAGAT GTTTGCCTCT 
TTTATCGACA GTAAGATTCT GTGCCACGAT GACGAGGACA AAGAGCACAC ACTGCGAGTC 
TTTGATTCCC GTGTTGACAA GATCCGCATG ATAAATGTCA GGACACCCAC CCTGCGGACC 
TCCATGTACC AGAAATGCAC CAATATCGAT GAAGCAGAAA AAGCCATAGA GGTGAGACTA 
TGTAAGATCG ACCACACGGC TCTGCATCCC CATCTGCTGG ACATGAAGAT CGGTCAGGGC 
CGCTATGAGC CAGGCTTTTT CCCTCGCTTA CAGTCTGATG TGCTCTCCAC TGGACCCACC 
AGCAACAAGT GGGCGAAGCG CAGTGCACCG GCCCAATGGC GGAGGAAAGA CCGACAGAAA 
CAGCACGCCG AACATCTCTA TCTGGACAAT GACCAGAGAG AGCATGTGGA GTGTCTGTTT 
TCTGAGCTTC AGCTTCTGCT GGCTCTGGAC TACTACTGGT TTTCGCAGTC CTTCAGGCCC 
TGTCTGAAGT CCGTCAAGGT GGATGTGAAA TACATCCAGG AAGCCAGAAA CCTTGGCACC 
ACACTCCGTC AGCCAAAACT CTCCAACCTG TCGCCTTCGG TCATTGCACA GACCAACTGG 
AAGTTTGTTG AGGGACTGTT GAAGGAGTGC AGAAATAAGA CAAAGCGCAT GCTGGTGGAG 
AAGATGGGAC GGGAGGCAGT GGAGCTGGGC CACGGTGAGG TGAGCATCAC CGGTGTGGAG 
GAAAACACAC TCATCGCCAG CCTGTGTGAC CTGCTGGAGC GAATCTGGAG CCACGGCCTA 
CAGGTCAAAC AGGGAAAATC CGCCCTGTGG TCGCACCTTT TACATTATCA AGAGAGTAAA 
GAGAAGAGAG ACGCTACTCC AGCAGGCCTC GGCCCTCCTG GTATCATTCA TGACACAGAG 
AGACGGAAGT CTGATGCTAG CTGTGCGATG CCGCCTCTCA AAGTCTCCTT GATTCAGGAC 
ATGAGGCACA TCCAGAACAT CAGCGAGATC AAGACGGACG TTGGCAAGGC CAGGGCTTGG 
GTGCGTCTCT CCATGGAGAA GAAACTGCTG TCCAGACACC TGAAGCAACT GCTGTCTGAC 
CACGAGCTCA CCAAGAAACT CTATAAGCGA TATGCTTTTC TGCGCTGTGA TGACGAGAAG 
GAGCAGTTCC TCTACCATCT CCTCTCTTTC AACGCTGTGG ACTACTTCTG CTTCACTAAT 
GTCTTCACCA CAATTATGAT TCCATACCAT GTGGTCATCA TTCCTAGTAA GAAACTCGGT 
GGCTCTATGT TCACAGCCAA CCCCTGGGTG TGTGTCTCAG GGGAACTAGC CGAGACTGGG 
GTCCTGCAGG TCCCTAAAAA CACACTGGAG ATTACATTTG AGTGCCAGAA TCTGGGGAAG 
CTGACCACTG TACAGATGGG CCATGATAAC TCAGGGCTGT ATGCTAAATG GCTAGTGGAG 
TGCGTAATGG TCCGCAATGG GATTACAGGA CACACCTATA AGTTTCCATG TGGCCGTTGG 
CTGGGTAAAG GAGTAGATGA TGGCAGCTTG GAGAGGATCC TGGTGGGCGA ATTGGTGACT 
AACATGGAGA GCGATGAAAG AATGTGCCGG ACTCCTCCGA TGCAGCAATC CCCCGGGATG 
ATGCGAAGAT TTGTCACCAT TTCGCCAAAC AGCAAACCAA AGTTAAACAC GGGCCAGATC 
CAGGAAAGTG TGGGAGAGGC GATTAACGGG ATCGTCAAAC ACTTCCATAA GCCGGAGAAA 
GAGAGAGGAA GCCTCACGTT GCTTCTGTGT GGGGAGTATG GTTTGGTTTG GGCTCTGGAG 
CAGGTGTTTC AGCATGGCTT CAAGTCACCG CGACTGTTCA AGAACGTCTT CATTTGGGAC 
TTTCTAGAAA AGGCGCAGGT TTACTTTGAG AGTGCGGAGC GGAGTCAGAT GACCCAGGAT 
GAGAACTGGC AGATGAGGGT CCGTCATTTC TGCCATTTCA TGCGCGCCAT CAACAGCACA 
CCCAGAAACA TCGGCAAAGA TGGGAAGTTT CAGATGCTTG TGTGTCTTGG GGCCAGAGAT 
CACCTGCTCC ACCATTGGAT CGCGCTTCTT GCCGAGTGTC CAATCACAGC ACAGATGTAC 
GAGGACGTGG CGCTCTTAAA GGACCATTCA TTGGTGAACT CTCTGATTCG AGTACTACAG 
ACTCTGCAGG AGTTCAATAT CACTCTGGAA GCTTCTCTCG TCAAAGGGAT TGGGATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009301847.1
CDS	210..4109
Translation	MSTGFSSSSCRFADYFVICGLDTESGLEPDELSGENFEQSPLRRTFKSKVLAHYPDNVEWNPFDQDAVGMLCMPKGLSFRTQTDTREPQFHSFIITREDGSRTYGFALTFFEEVTSKQICSAMQTLYHMHNAEQYDILHTPPTPRSPEDPRPTTQQLHASPALSRLQRFNSYDISRDTLYVSKCICLITPMAFAHACRKVLEQLHQAVTSPQPPPLPLESYVYNVLYEVPLPPAGRSLKFSGVYRPVICQRPSTAELPLFDFPLQEVFELLGLENILQLFTCALLEIQILLYSQHYQRLMTVAESITALMFPFQWQHVYVPILPASLLHFLDAPVPYLMGLHSNGQDDRTKLELPQEANLCFVDIDNHFIELPEDLPQFPNKLEFIQEISEVLLAFGLSPEANPQCSEGQSKIRGFRCGDMASDKRNGNLAGSPLNSFLLRENQTIARLQALVKRTGVSLEKLDVKDDASTNKDTKVQCEEEEMKMHQLNIRVREVFANRFSQMFSDYEVFVIQPSQDKESWFSNRDQMQNFDKASFLSDQPEPYLPFLSRFLETQMFASFIDSKILCHDDEDKEHTLRVFDSRVDKIRMINVRTPTLRTSMYQKCTNIDEAEKAIEVRLCKIDHTALHPHLLDMKIGQGRYEPGFFPRLQSDVLSTGPTSNKWAKRSAPAQWRRKDRQKQHAEHLYLDNDQREHVECLFSELQLLLALDYYWFSQSFRPCLKSVKVDVKYIQEARNLGTTLRQPKLSNLSPSVIAQTNWKFVEGLLKECRNKTKRMLVEKMGREAVELGHGEVSITGVEENTLIASLCDLLERIWSHGLQVKQGKSALWSHLLHYQESKEKRDATPAGLGPPGIIHDTERRKSDASCAMPPLKVSLIQDMRHIQNISEIKTDVGKARAWVRLSMEKKLLSRHLKQLLSDHELTKKLYKRYAFLRCDDEKEQFLYHLLSFNAVDYFCFTNVFTTIMIPYHVVIIPSKKLGGSMFTANPWVCVSGELAETGVLQVPKNTLEITFECQNLGKLTTVQMGHDNSGLYAKWLVECVMVRNGITGHTYKFPCGRWLGKGVDDGSLERILVGELVTNMESDERMCRTPPMQQSPGMMRRFVTISPNSKPKLNTGQIQESVGEAINGIVKHFHKPEKERGSLTLLLCGEYGLVWALEQVFQHGFKSPRLFKNVFIWDFLEKAQVYFESAERSQMTQDENWQMRVRHFCHFMRAINSTPRNIGKDGKFQMLVCLGARDHLLHHWIALLAECPITAQMYEDVALLKDHSLVNSLIRVLQTLQEFNITLEASLVKGIGI

Target ORF information:

RefSeq Version	XM_009303572.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio DENN/MADD domain containing 5A (dennd5a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009303572.3

ORF Insert Sequence:

ATGAGCACCG GATTCAGCTC CAGCTCCTGC CGCTTCGCGG ATTATTTCGT GATCTGCGGT 
CTGGACACCG AGAGCGGCTT AGAACCAGAC GAGCTGTCCG GTGAGAATTT TGAGCAGAGT 
CCACTGCGGA GAACGTTTAA ATCGAAGGTT CTAGCGCATT ATCCTGACAA TGTGGAGTGG 
AACCCTTTTG ACCAGGATGC AGTGGGAATG CTCTGTATGC CAAAAGGCCT GTCCTTCCGC 
ACTCAAACAG ACACCCGTGA GCCCCAGTTC CACTCCTTCA TTATCACCAG AGAGGACGGC 
TCCCGGACGT ACGGCTTTGC CCTCACTTTC TTTGAGGAGG TGACCAGCAA ACAGATCTGC 
AGTGCCATGC AGACACTTTA TCACATGCAC AATGCGGAAC AGTATGATAT CCTGCACACA 
CCACCCACGC CTCGCAGTCC AGAAGATCCC CGTCCCACCA CGCAACAACT GCACGCCTCC 
CCTGCTCTGT CCAGGCTGCA GCGCTTCAAC TCATACGACA TCAGCCGGGA CACGCTGTAC 
GTGTCCAAAT GCATCTGCCT GATCACCCCC ATGGCCTTCG CCCACGCCTG CAGGAAGGTT 
CTGGAGCAGC TGCATCAGGC CGTCACCTCA CCTCAGCCCC CGCCGCTGCC GCTGGAGAGC 
TATGTCTACA ATGTCCTCTA TGAGGTCCCA CTGCCACCAG CCGGACGCTC CTTGAAGTTT 
TCAGGCGTCT ACAGGCCAGT GATCTGTCAG AGACCCAGCA CAGCCGAGCT GCCCCTCTTT 
GACTTCCCCC TTCAGGAGGT GTTTGAGCTG CTAGGGCTGG AGAACATCTT GCAGCTCTTC 
ACCTGTGCGC TGCTGGAGAT CCAGATCCTT CTGTACTCAC AACATTACCA GCGGCTGATG 
ACGGTGGCGG AGAGCATCAC AGCCTTAATG TTCCCCTTTC AGTGGCAGCA TGTGTACGTG 
CCCATCCTGC CCGCTTCCCT GCTCCACTTC CTGGACGCTC CTGTGCCGTA CCTCATGGGT 
CTGCACTCTA ACGGCCAGGA TGACCGCACC AAACTGGAGC TTCCCCAAGA GGCCAATCTC 
TGCTTTGTCG ATATTGACAA CCACTTCATC GAGCTACCCG AAGACCTGCC TCAGTTCCCC 
AACAAGCTGG AGTTTATCCA GGAGATCTCT GAGGTTCTGC TGGCGTTTGG CTTGTCTCCA 
GAAGCGAACC CACAATGCAG CGAGGGCCAG AGCAAGATCA GGGGCTTCCG GTGTGGCGAC 
ATGGCCTCCG ACAAGCGCAA CGGGAACCTG GCCGGCTCGC CGCTCAATTC GTTCCTGCTG 
AGAGAAAACC AAACCATCGC TCGCCTGCAA GCGCTGGTCA AGAGGACTGG AGTCAGTCTG 
GAGAAGTTGG ACGTGAAGGA CGACGCAAGC ACTAATAAGG ATACGAAAGT GCAGTGTGAA 
GAAGAAGAGA TGAAAATGCA CCAGCTGAAC ATTCGGGTGC GAGAGGTCTT CGCCAACCGT 
TTCTCTCAGA TGTTCTCGGA TTATGAGGTG TTTGTTATTC AGCCCAGCCA AGACAAAGAG 
TCCTGGTTCA GCAACCGAGA CCAGATGCAG AACTTTGACA AGGCCTCCTT CCTGTCAGAT 
CAGCCCGAAC CGTACCTGCC GTTCCTCTCC CGCTTTCTGG AGACGCAGAT GTTTGCCTCT 
TTTATCGACA GTAAGATTCT GTGCCACGAT GACGAGGACA AAGAGCACAC ACTGCGAGTC 
TTTGATTCCC GTGTTGACAA GATCCGCATG ATAAATGTCA GGACACCCAC CCTGCGGACC 
TCCATGTACC AGAAATGCAC CAATATCGAT GAAGCAGAAA AAGCCATAGA GGTGAGACTA 
TGTAAGATCG ACCACACGGC TCTGCATCCC CATCTGCTGG ACATGAAGAT CGGTCAGGGC 
CGCTATGAGC CAGGCTTTTT CCCTCGCTTA CAGTCTGATG TGCTCTCCAC TGGACCCACC 
AGCAACAAGT GGGCGAAGCG CAGTGCACCG GCCCAATGGC GGAGGAAAGA CCGACAGAAA 
CAGCACGCCG AACATCTCTA TCTGGACAAT GACCAGAGAG AGCATGTGGA GTGTCTGTTT 
TCTGAGCTTC AGCTTCTGCT GGCTCTGGAC TACTACTGGT TTTCGCAGTC CTTCAGGCCC 
TGTCTGAAGT CCGTCAAGGT GGATGTGAAA TACATCCAGG AAGCCAGAAA CCTTGGCACC 
ACACTCCGTC AGCCAAAACT CTCCAACCTG TCGCCTTCGG TCATTGCACA GACCAACTGG 
AAGTTTGTTG AGGGACTGTT GAAGGAGTGC AGAAATAAGA CAAAGCGCAT GCTGGTGGAG 
AAGATGGGAC GGGAGGCAGT GGAGCTGGGC CACGGTGAGG TGAGCATCAC CGGTGTGGAG 
GAAAACACAC TCATCGCCAG CCTGTGTGAC CTGCTGGAGC GAATCTGGAG CCACGGCCTA 
CAGGTCAAAC AGGGAAAATC CGCCCTGTGG TCGCACCTTT TACATTATCA AGAGAGTAAA 
GAGAAGAGAG ACGCTACTCC AGCAGGCCTC GGCCCTCCTG GTATCATTCA TGACACAGAG 
AGACGGAAGT CTGATGCTAG CTGTGCGATG CCGCCTCTCA AAGTCTCCTT GATTCAGGAC 
ATGAGGCACA TCCAGAACAT CAGCGAGATC AAGACGGACG TTGGCAAGGC CAGGGCTTGG 
GTGCGTCTCT CCATGGAGAA GAAACTGCTG TCCAGACACC TGAAGCAACT GCTGTCTGAC 
CACGAGCTCA CCAAGAAACT CTATAAGCGA TATGCTTTTC TGCGCTGTGA TGACGAGAAG 
GAGCAGTTCC TCTACCATCT CCTCTCTTTC AACGCTGTGG ACTACTTCTG CTTCACTAAT 
GTCTTCACCA CAATTATGAT TCCATACCAT GTGGTCATCA TTCCTAGTAA GAAACTCGGT 
GGCTCTATGT TCACAGCCAA CCCCTGGGTG TGTGTCTCAG GGGAACTAGC CGAGACTGGG 
GTCCTGCAGG TCCCTAAAAA CACACTGGAG ATTACATTTG AGTGCCAGAA TCTGGGGAAG 
CTGACCACTG TACAGATGGG CCATGATAAC TCAGGGCTGT ATGCTAAATG GCTAGTGGAG 
TGCGTAATGG TCCGCAATGG GATTACAGGA CACACCTATA AGTTTCCATG TGGCCGTTGG 
CTGGGTAAAG GAGTAGATGA TGGCAGCTTG GAGAGGATCC TGGTGGGCGA ATTGGTGACT 
AACATGGAGA GCGATGAAAG AATGTGCCGG ACTCCTCCGA TGCAGCAATC CCCCGGGATG 
ATGCGAAGAT TTGTCACCAT TTCGCCAAAC AGCAAACCAA AGTTAAACAC GGGCCAGATC 
CAGGAAAGTG TGGGAGAGGC GATTAACGGG ATCGTCAAAC ACTTCCATAA GCCGGAGAAA 
GAGAGAGGAA GCCTCACGTT GCTTCTGTGT GGGGAGTATG GTTTGGTTTG GGCTCTGGAG 
CAGGTGTTTC AGCATGGCTT CAAGTCACCG CGACTGTTCA AGAACGTCTT CATTTGGGAC 
TTTCTAGAAA AGGCGCAGGT TTACTTTGAG AGTGCGGAGC GGAGTCAGAT GACCCAGGAT 
GAGAACTGGC AGATGAGGGT CCGTCATTTC TGCCATTTCA TGCGCGCCAT CAACAGCACA 
CCCAGAAACA TCGGCAAAGA TGGGAAGTTT CAGATGCTTG TGTGTCTTGG GGCCAGAGAT 
CACCTGCTCC ACCATTGGAT CGCGCTTCTT GCCGAGTGTC CAATCACAGC ACAGATGTAC 
GAGGACGTGG CGCTCTTAAA GGACCATTCA TTGGTGAACT CTCTGATTCG AGTACTACAG 
ACTCTGCAGG AGTTCAATAT CACTCTGGAA GCTTCTCTCG TCAAAGGGAT TGGGATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dennd5a ( XM_009303572.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009301847.1 Danio rerio DENN/MADD domain containing 5A (dennd5a), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa47408
Clone ID Related Accession (Same CDS sequence)	XM_009303572.3 , XM_009303572.2
Accession Version	XM_009303572.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3900bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	DENN domain-containing protein 5A isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009303572.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCACCG GATTCAGCTC CAGCTCCTGC CGCTTCGCGG ATTATTTCGT GATCTGCGGT 
CTGGACACCG AGAGCGGCTT AGAACCAGAC GAGCTGTCCG GTGAGAATTT TGAGCAGAGT 
CCACTGCGGA GAACGTTTAA ATCGAAGGTT CTAGCGCATT ATCCTGACAA TGTGGAGTGG 
AACCCTTTTG ACCAGGATGC AGTGGGAATG CTCTGTATGC CAAAAGGCCT GTCCTTCCGC 
ACTCAAACAG ACACCCGTGA GCCCCAGTTC CACTCCTTCA TTATCACCAG AGAGGACGGC 
TCCCGGACGT ACGGCTTTGC CCTCACTTTC TTTGAGGAGG TGACCAGCAA ACAGATCTGC 
AGTGCCATGC AGACACTTTA TCACATGCAC AATGCGGAAC AGTATGATAT CCTGCACACA 
CCACCCACGC CTCGCAGTCC AGAAGATCCC CGTCCCACCA CGCAACAACT GCACGCCTCC 
CCTGCTCTGT CCAGGCTGCA GCGCTTCAAC TCATACGACA TCAGCCGGGA CACGCTGTAC 
GTGTCCAAAT GCATCTGCCT GATCACCCCC ATGGCCTTCG CCCACGCCTG CAGGAAGGTT 
CTGGAGCAGC TGCATCAGGC CGTCACCTCA CCTCAGCCCC CGCCGCTGCC GCTGGAGAGC 
TATGTCTACA ATGTCCTCTA TGAGGTCCCA CTGCCACCAG CCGGACGCTC CTTGAAGTTT 
TCAGGCGTCT ACAGGCCAGT GATCTGTCAG AGACCCAGCA CAGCCGAGCT GCCCCTCTTT 
GACTTCCCCC TTCAGGAGGT GTTTGAGCTG CTAGGGCTGG AGAACATCTT GCAGCTCTTC 
ACCTGTGCGC TGCTGGAGAT CCAGATCCTT CTGTACTCAC AACATTACCA GCGGCTGATG 
ACGGTGGCGG AGAGCATCAC AGCCTTAATG TTCCCCTTTC AGTGGCAGCA TGTGTACGTG 
CCCATCCTGC CCGCTTCCCT GCTCCACTTC CTGGACGCTC CTGTGCCGTA CCTCATGGGT 
CTGCACTCTA ACGGCCAGGA TGACCGCACC AAACTGGAGC TTCCCCAAGA GGCCAATCTC 
TGCTTTGTCG ATATTGACAA CCACTTCATC GAGCTACCCG AAGACCTGCC TCAGTTCCCC 
AACAAGCTGG AGTTTATCCA GGAGATCTCT GAGGTTCTGC TGGCGTTTGG CTTGTCTCCA 
GAAGCGAACC CACAATGCAG CGAGGGCCAG AGCAAGATCA GGGGCTTCCG GTGTGGCGAC 
ATGGCCTCCG ACAAGCGCAA CGGGAACCTG GCCGGCTCGC CGCTCAATTC GTTCCTGCTG 
AGAGAAAACC AAACCATCGC TCGCCTGCAA GCGCTGGTCA AGAGGACTGG AGTCAGTCTG 
GAGAAGTTGG ACGTGAAGGA CGACGCAAGC ACTAATAAGG ATACGAAAGT GCAGTGTGAA 
GAAGAAGAGA TGAAAATGCA CCAGCTGAAC ATTCGGGTGC GAGAGGTCTT CGCCAACCGT 
TTCTCTCAGA TGTTCTCGGA TTATGAGGTG TTTGTTATTC AGCCCAGCCA AGACAAAGAG 
TCCTGGTTCA GCAACCGAGA CCAGATGCAG AACTTTGACA AGGCCTCCTT CCTGTCAGAT 
CAGCCCGAAC CGTACCTGCC GTTCCTCTCC CGCTTTCTGG AGACGCAGAT GTTTGCCTCT 
TTTATCGACA GTAAGATTCT GTGCCACGAT GACGAGGACA AAGAGCACAC ACTGCGAGTC 
TTTGATTCCC GTGTTGACAA GATCCGCATG ATAAATGTCA GGACACCCAC CCTGCGGACC 
TCCATGTACC AGAAATGCAC CAATATCGAT GAAGCAGAAA AAGCCATAGA GGTGAGACTA 
TGTAAGATCG ACCACACGGC TCTGCATCCC CATCTGCTGG ACATGAAGAT CGGTCAGGGC 
CGCTATGAGC CAGGCTTTTT CCCTCGCTTA CAGTCTGATG TGCTCTCCAC TGGACCCACC 
AGCAACAAGT GGGCGAAGCG CAGTGCACCG GCCCAATGGC GGAGGAAAGA CCGACAGAAA 
CAGCACGCCG AACATCTCTA TCTGGACAAT GACCAGAGAG AGCATGTGGA GTGTCTGTTT 
TCTGAGCTTC AGCTTCTGCT GGCTCTGGAC TACTACTGGT TTTCGCAGTC CTTCAGGCCC 
TGTCTGAAGT CCGTCAAGGT GGATGTGAAA TACATCCAGG AAGCCAGAAA CCTTGGCACC 
ACACTCCGTC AGCCAAAACT CTCCAACCTG TCGCCTTCGG TCATTGCACA GACCAACTGG 
AAGTTTGTTG AGGGACTGTT GAAGGAGTGC AGAAATAAGA CAAAGCGCAT GCTGGTGGAG 
AAGATGGGAC GGGAGGCAGT GGAGCTGGGC CACGGTGAGG TGAGCATCAC CGGTGTGGAG 
GAAAACACAC TCATCGCCAG CCTGTGTGAC CTGCTGGAGC GAATCTGGAG CCACGGCCTA 
CAGGTCAAAC AGGGAAAATC CGCCCTGTGG TCGCACCTTT TACATTATCA AGAGAGTAAA 
GAGAAGAGAG ACGCTACTCC AGCAGGCCTC GGCCCTCCTG GTATCATTCA TGACACAGAG 
AGACGGAAGT CTGATGCTAG CTGTGCGATG CCGCCTCTCA AAGTCTCCTT GATTCAGGAC 
ATGAGGCACA TCCAGAACAT CAGCGAGATC AAGACGGACG TTGGCAAGGC CAGGGCTTGG 
GTGCGTCTCT CCATGGAGAA GAAACTGCTG TCCAGACACC TGAAGCAACT GCTGTCTGAC 
CACGAGCTCA CCAAGAAACT CTATAAGCGA TATGCTTTTC TGCGCTGTGA TGACGAGAAG 
GAGCAGTTCC TCTACCATCT CCTCTCTTTC AACGCTGTGG ACTACTTCTG CTTCACTAAT 
GTCTTCACCA CAATTATGAT TCCATACCAT GTGGTCATCA TTCCTAGTAA GAAACTCGGT 
GGCTCTATGT TCACAGCCAA CCCCTGGGTG TGTGTCTCAG GGGAACTAGC CGAGACTGGG 
GTCCTGCAGG TCCCTAAAAA CACACTGGAG ATTACATTTG AGTGCCAGAA TCTGGGGAAG 
CTGACCACTG TACAGATGGG CCATGATAAC TCAGGGCTGT ATGCTAAATG GCTAGTGGAG 
TGCGTAATGG TCCGCAATGG GATTACAGGA CACACCTATA AGTTTCCATG TGGCCGTTGG 
CTGGGTAAAG GAGTAGATGA TGGCAGCTTG GAGAGGATCC TGGTGGGCGA ATTGGTGACT 
AACATGGAGA GCGATGAAAG AATGTGCCGG ACTCCTCCGA TGCAGCAATC CCCCGGGATG 
ATGCGAAGAT TTGTCACCAT TTCGCCAAAC AGCAAACCAA AGTTAAACAC GGGCCAGATC 
CAGGAAAGTG TGGGAGAGGC GATTAACGGG ATCGTCAAAC ACTTCCATAA GCCGGAGAAA 
GAGAGAGGAA GCCTCACGTT GCTTCTGTGT GGGGAGTATG GTTTGGTTTG GGCTCTGGAG 
CAGGTGTTTC AGCATGGCTT CAAGTCACCG CGACTGTTCA AGAACGTCTT CATTTGGGAC 
TTTCTAGAAA AGGCGCAGGT TTACTTTGAG AGTGCGGAGC GGAGTCAGAT GACCCAGGAT 
GAGAACTGGC AGATGAGGGT CCGTCATTTC TGCCATTTCA TGCGCGCCAT CAACAGCACA 
CCCAGAAACA TCGGCAAAGA TGGGAAGTTT CAGATGCTTG TGTGTCTTGG GGCCAGAGAT 
CACCTGCTCC ACCATTGGAT CGCGCTTCTT GCCGAGTGTC CAATCACAGC ACAGATGTAC 
GAGGACGTGG CGCTCTTAAA GGACCATTCA TTGGTGAACT CTCTGATTCG AGTACTACAG 
ACTCTGCAGG AGTTCAATAT CACTCTGGAA GCTTCTCTCG TCAAAGGGAT TGGGATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009301847.1
CDS	205..4104
Translation	MSTGFSSSSCRFADYFVICGLDTESGLEPDELSGENFEQSPLRRTFKSKVLAHYPDNVEWNPFDQDAVGMLCMPKGLSFRTQTDTREPQFHSFIITREDGSRTYGFALTFFEEVTSKQICSAMQTLYHMHNAEQYDILHTPPTPRSPEDPRPTTQQLHASPALSRLQRFNSYDISRDTLYVSKCICLITPMAFAHACRKVLEQLHQAVTSPQPPPLPLESYVYNVLYEVPLPPAGRSLKFSGVYRPVICQRPSTAELPLFDFPLQEVFELLGLENILQLFTCALLEIQILLYSQHYQRLMTVAESITALMFPFQWQHVYVPILPASLLHFLDAPVPYLMGLHSNGQDDRTKLELPQEANLCFVDIDNHFIELPEDLPQFPNKLEFIQEISEVLLAFGLSPEANPQCSEGQSKIRGFRCGDMASDKRNGNLAGSPLNSFLLRENQTIARLQALVKRTGVSLEKLDVKDDASTNKDTKVQCEEEEMKMHQLNIRVREVFANRFSQMFSDYEVFVIQPSQDKESWFSNRDQMQNFDKASFLSDQPEPYLPFLSRFLETQMFASFIDSKILCHDDEDKEHTLRVFDSRVDKIRMINVRTPTLRTSMYQKCTNIDEAEKAIEVRLCKIDHTALHPHLLDMKIGQGRYEPGFFPRLQSDVLSTGPTSNKWAKRSAPAQWRRKDRQKQHAEHLYLDNDQREHVECLFSELQLLLALDYYWFSQSFRPCLKSVKVDVKYIQEARNLGTTLRQPKLSNLSPSVIAQTNWKFVEGLLKECRNKTKRMLVEKMGREAVELGHGEVSITGVEENTLIASLCDLLERIWSHGLQVKQGKSALWSHLLHYQESKEKRDATPAGLGPPGIIHDTERRKSDASCAMPPLKVSLIQDMRHIQNISEIKTDVGKARAWVRLSMEKKLLSRHLKQLLSDHELTKKLYKRYAFLRCDDEKEQFLYHLLSFNAVDYFCFTNVFTTIMIPYHVVIIPSKKLGGSMFTANPWVCVSGELAETGVLQVPKNTLEITFECQNLGKLTTVQMGHDNSGLYAKWLVECVMVRNGITGHTYKFPCGRWLGKGVDDGSLERILVGELVTNMESDERMCRTPPMQQSPGMMRRFVTISPNSKPKLNTGQIQESVGEAINGIVKHFHKPEKERGSLTLLLCGEYGLVWALEQVFQHGFKSPRLFKNVFIWDFLEKAQVYFESAERSQMTQDENWQMRVRHFCHFMRAINSTPRNIGKDGKFQMLVCLGARDHLLHHWIALLAECPITAQMYEDVALLKDHSLVNSLIRVLQTLQEFNITLEASLVKGIGI

Target ORF information:

RefSeq Version	XM_009303572.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio DENN/MADD domain containing 5A (dennd5a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009303572.2

ORF Insert Sequence:

ATGAGCACCG GATTCAGCTC CAGCTCCTGC CGCTTCGCGG ATTATTTCGT GATCTGCGGT 
CTGGACACCG AGAGCGGCTT AGAACCAGAC GAGCTGTCCG GTGAGAATTT TGAGCAGAGT 
CCACTGCGGA GAACGTTTAA ATCGAAGGTT CTAGCGCATT ATCCTGACAA TGTGGAGTGG 
AACCCTTTTG ACCAGGATGC AGTGGGAATG CTCTGTATGC CAAAAGGCCT GTCCTTCCGC 
ACTCAAACAG ACACCCGTGA GCCCCAGTTC CACTCCTTCA TTATCACCAG AGAGGACGGC 
TCCCGGACGT ACGGCTTTGC CCTCACTTTC TTTGAGGAGG TGACCAGCAA ACAGATCTGC 
AGTGCCATGC AGACACTTTA TCACATGCAC AATGCGGAAC AGTATGATAT CCTGCACACA 
CCACCCACGC CTCGCAGTCC AGAAGATCCC CGTCCCACCA CGCAACAACT GCACGCCTCC 
CCTGCTCTGT CCAGGCTGCA GCGCTTCAAC TCATACGACA TCAGCCGGGA CACGCTGTAC 
GTGTCCAAAT GCATCTGCCT GATCACCCCC ATGGCCTTCG CCCACGCCTG CAGGAAGGTT 
CTGGAGCAGC TGCATCAGGC CGTCACCTCA CCTCAGCCCC CGCCGCTGCC GCTGGAGAGC 
TATGTCTACA ATGTCCTCTA TGAGGTCCCA CTGCCACCAG CCGGACGCTC CTTGAAGTTT 
TCAGGCGTCT ACAGGCCAGT GATCTGTCAG AGACCCAGCA CAGCCGAGCT GCCCCTCTTT 
GACTTCCCCC TTCAGGAGGT GTTTGAGCTG CTAGGGCTGG AGAACATCTT GCAGCTCTTC 
ACCTGTGCGC TGCTGGAGAT CCAGATCCTT CTGTACTCAC AACATTACCA GCGGCTGATG 
ACGGTGGCGG AGAGCATCAC AGCCTTAATG TTCCCCTTTC AGTGGCAGCA TGTGTACGTG 
CCCATCCTGC CCGCTTCCCT GCTCCACTTC CTGGACGCTC CTGTGCCGTA CCTCATGGGT 
CTGCACTCTA ACGGCCAGGA TGACCGCACC AAACTGGAGC TTCCCCAAGA GGCCAATCTC 
TGCTTTGTCG ATATTGACAA CCACTTCATC GAGCTACCCG AAGACCTGCC TCAGTTCCCC 
AACAAGCTGG AGTTTATCCA GGAGATCTCT GAGGTTCTGC TGGCGTTTGG CTTGTCTCCA 
GAAGCGAACC CACAATGCAG CGAGGGCCAG AGCAAGATCA GGGGCTTCCG GTGTGGCGAC 
ATGGCCTCCG ACAAGCGCAA CGGGAACCTG GCCGGCTCGC CGCTCAATTC GTTCCTGCTG 
AGAGAAAACC AAACCATCGC TCGCCTGCAA GCGCTGGTCA AGAGGACTGG AGTCAGTCTG 
GAGAAGTTGG ACGTGAAGGA CGACGCAAGC ACTAATAAGG ATACGAAAGT GCAGTGTGAA 
GAAGAAGAGA TGAAAATGCA CCAGCTGAAC ATTCGGGTGC GAGAGGTCTT CGCCAACCGT 
TTCTCTCAGA TGTTCTCGGA TTATGAGGTG TTTGTTATTC AGCCCAGCCA AGACAAAGAG 
TCCTGGTTCA GCAACCGAGA CCAGATGCAG AACTTTGACA AGGCCTCCTT CCTGTCAGAT 
CAGCCCGAAC CGTACCTGCC GTTCCTCTCC CGCTTTCTGG AGACGCAGAT GTTTGCCTCT 
TTTATCGACA GTAAGATTCT GTGCCACGAT GACGAGGACA AAGAGCACAC ACTGCGAGTC 
TTTGATTCCC GTGTTGACAA GATCCGCATG ATAAATGTCA GGACACCCAC CCTGCGGACC 
TCCATGTACC AGAAATGCAC CAATATCGAT GAAGCAGAAA AAGCCATAGA GGTGAGACTA 
TGTAAGATCG ACCACACGGC TCTGCATCCC CATCTGCTGG ACATGAAGAT CGGTCAGGGC 
CGCTATGAGC CAGGCTTTTT CCCTCGCTTA CAGTCTGATG TGCTCTCCAC TGGACCCACC 
AGCAACAAGT GGGCGAAGCG CAGTGCACCG GCCCAATGGC GGAGGAAAGA CCGACAGAAA 
CAGCACGCCG AACATCTCTA TCTGGACAAT GACCAGAGAG AGCATGTGGA GTGTCTGTTT 
TCTGAGCTTC AGCTTCTGCT GGCTCTGGAC TACTACTGGT TTTCGCAGTC CTTCAGGCCC 
TGTCTGAAGT CCGTCAAGGT GGATGTGAAA TACATCCAGG AAGCCAGAAA CCTTGGCACC 
ACACTCCGTC AGCCAAAACT CTCCAACCTG TCGCCTTCGG TCATTGCACA GACCAACTGG 
AAGTTTGTTG AGGGACTGTT GAAGGAGTGC AGAAATAAGA CAAAGCGCAT GCTGGTGGAG 
AAGATGGGAC GGGAGGCAGT GGAGCTGGGC CACGGTGAGG TGAGCATCAC CGGTGTGGAG 
GAAAACACAC TCATCGCCAG CCTGTGTGAC CTGCTGGAGC GAATCTGGAG CCACGGCCTA 
CAGGTCAAAC AGGGAAAATC CGCCCTGTGG TCGCACCTTT TACATTATCA AGAGAGTAAA 
GAGAAGAGAG ACGCTACTCC AGCAGGCCTC GGCCCTCCTG GTATCATTCA TGACACAGAG 
AGACGGAAGT CTGATGCTAG CTGTGCGATG CCGCCTCTCA AAGTCTCCTT GATTCAGGAC 
ATGAGGCACA TCCAGAACAT CAGCGAGATC AAGACGGACG TTGGCAAGGC CAGGGCTTGG 
GTGCGTCTCT CCATGGAGAA GAAACTGCTG TCCAGACACC TGAAGCAACT GCTGTCTGAC 
CACGAGCTCA CCAAGAAACT CTATAAGCGA TATGCTTTTC TGCGCTGTGA TGACGAGAAG 
GAGCAGTTCC TCTACCATCT CCTCTCTTTC AACGCTGTGG ACTACTTCTG CTTCACTAAT 
GTCTTCACCA CAATTATGAT TCCATACCAT GTGGTCATCA TTCCTAGTAA GAAACTCGGT 
GGCTCTATGT TCACAGCCAA CCCCTGGGTG TGTGTCTCAG GGGAACTAGC CGAGACTGGG 
GTCCTGCAGG TCCCTAAAAA CACACTGGAG ATTACATTTG AGTGCCAGAA TCTGGGGAAG 
CTGACCACTG TACAGATGGG CCATGATAAC TCAGGGCTGT ATGCTAAATG GCTAGTGGAG 
TGCGTAATGG TCCGCAATGG GATTACAGGA CACACCTATA AGTTTCCATG TGGCCGTTGG 
CTGGGTAAAG GAGTAGATGA TGGCAGCTTG GAGAGGATCC TGGTGGGCGA ATTGGTGACT 
AACATGGAGA GCGATGAAAG AATGTGCCGG ACTCCTCCGA TGCAGCAATC CCCCGGGATG 
ATGCGAAGAT TTGTCACCAT TTCGCCAAAC AGCAAACCAA AGTTAAACAC GGGCCAGATC 
CAGGAAAGTG TGGGAGAGGC GATTAACGGG ATCGTCAAAC ACTTCCATAA GCCGGAGAAA 
GAGAGAGGAA GCCTCACGTT GCTTCTGTGT GGGGAGTATG GTTTGGTTTG GGCTCTGGAG 
CAGGTGTTTC AGCATGGCTT CAAGTCACCG CGACTGTTCA AGAACGTCTT CATTTGGGAC 
TTTCTAGAAA AGGCGCAGGT TTACTTTGAG AGTGCGGAGC GGAGTCAGAT GACCCAGGAT 
GAGAACTGGC AGATGAGGGT CCGTCATTTC TGCCATTTCA TGCGCGCCAT CAACAGCACA 
CCCAGAAACA TCGGCAAAGA TGGGAAGTTT CAGATGCTTG TGTGTCTTGG GGCCAGAGAT 
CACCTGCTCC ACCATTGGAT CGCGCTTCTT GCCGAGTGTC CAATCACAGC ACAGATGTAC 
GAGGACGTGG CGCTCTTAAA GGACCATTCA TTGGTGAACT CTCTGATTCG AGTACTACAG 
ACTCTGCAGG AGTTCAATAT CACTCTGGAA GCTTCTCTCG TCAAAGGGAT TGGGATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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dennd5a cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Eメール

電話

FAX

オンラインフォーム

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