si:ch211-251j10.3 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	si:ch211-251j10.3
Entrez Gene ID	799483
Full Name	si:ch211-251j10.3
General protein information	Preferred Names si:ch211-251j10.3 Names ral guanine nucleotide dissociation stimulator
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001123317.1	NP_001116789.1	ral guanine nucleotide dissociation stimulator
XM_009304058.3	XP_009302333.1	ral guanine nucleotide dissociation stimulator isoform X3
XM_009304057.2	XP_009302332.1	ral guanine nucleotide dissociation stimulator isoform X2
XM_005167182.4	XP_005167239.1	ral guanine nucleotide dissociation stimulator isoform X1
XM_005167182.3	XP_005167239.1	ral guanine nucleotide dissociation stimulator isoform X1
XM_009304058.2	XP_009302333.1	ral guanine nucleotide dissociation stimulator isoform X3
XM_009304057.3	XP_009302332.1	ral guanine nucleotide dissociation stimulator isoform X2

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	RALGDS	XP_003312415.1
Bos taurus (cattle)	RALGDS	NP_001095448.1
Rattus norvegicus (Norway rat)	Ralgds	NP_062123.2
Danio rerio (zebrafish)	si:ch211-251j10.3	NP_001116789.1
Homo sapiens (human)	RALGDS	NP_001258705.1
Canis lupus familiaris (dog)	RALGDS	XP_005625225.1
Mus musculus (house mouse)	Ralgds	NP_001139308.1
Gallus gallus (chicken)	RALGDS	XP_004945986.1
	RALGDS	XP_001102309.2
Xenopus tropicalis (tropical clawed frog)	ralgds	NP_001107295.1

Gene Ontology
Bibliography

Related articles in PubMed

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Mutagenesis Screen Identifies agtpbp1 and eps15L1 as Essential for T lymphocyte Development in Zebrafish.
Seiler C, Gebhart N, Zhang Y, Shinton SA, Li YS, Ross NL, Liu X, Li Q, Bilbee AN, Varshney GK, LaFave MC, Burgess SM, Balciuniene J, Balciunas D, Hardy RR, Kappes DJ, Wiest DL, Rhodes J
PloS one10(7)e0131908(2015)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following si:ch211-251j10.3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the si:ch211-251j10.3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa31955	NM_001123317.1 Latest version!	Danio rerio si:ch211-251j10.3 (si:ch211-251j10.3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
ODa14382	XM_005167182.4 Latest version!	Danio rerio si:ch211-251j10.3 (si:ch211-251j10.3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
ODa47376	XM_009304057.3 Latest version!	Danio rerio si:ch211-251j10.3 (si:ch211-251j10.3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
ODa47377	XM_009304058.3 Latest version!	Danio rerio si:ch211-251j10.3 (si:ch211-251j10.3), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
View Old Accession Versions >>
ODa14382	XM_005167182.3	Danio rerio si:ch211-251j10.3 (si:ch211-251j10.3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
ODa47377	XM_009304058.2	Danio rerio si:ch211-251j10.3 (si:ch211-251j10.3), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
ODa47376	XM_009304057.2	Danio rerio si:ch211-251j10.3 (si:ch211-251j10.3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

si:ch211-251j10.3 ( NM_001123317.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001116789.1 Danio rerio si:ch211-251j10.3 (si:ch211-251j10.3), mRNA.

Price & Availability↑

CloneID	ODa31955
Clone ID Related Accession (Same CDS sequence)	NM_001123317.1
Accession Version	NM_001123317.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2442bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	ral guanine nucleotide dissociation stimulator
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BX640472.24. On Apr 11, 2008 this sequence version replaced XM_001339797.1. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGGTCTATA TTATGGGGAT ACAGTCGGAC GCCCTGGGTG TTAAACCCAC GTCCCTGGAT 
GAGCTGTTTG AGTCCAGTAC ATGGAGAATT AAAAACATAT GGGATGGGGT GAAGCTGGAG 
ATCGCGGCCG GAGATGGATG TCCGGTGGTC TTAAATAGTT TTACGCATTT GGACCCAGAT 
CTACCTATGT TGGAGAGCTC AGTTCAGGAG ATTGGAGAGG AGGTGGAGGA CGGTGCCATC 
TATACCATTA CACTCCGAAA GGTGCAGCTC CACCAGACGG CCAGTAAGGG CCAGCGATGG 
CTGGGCGTGG AGACAGACTC TGCCCTCAGT CTGTACGAGA CGTGCAAGGG TCGAACCATT 
AAGGCTGGCA CACTGGAGAA ACTAGTGGAG TACATGGTCT CCGCCTTCAA AGGGAAAGAC 
TACACTTACG TCACTATTTT CCTCTGCACC TACAGAGCAT TTGCCACCAC CAAACAAGTG 
CTAGACCTGC TGCTTAACAG ATATGCCAAA CTGCCAGCCT CAGGCAAGAC TAAAGTCTCA 
GCAGAAGACC ACACAGAGCT TAAAAACACT GTGTCATCTA TCCTGGGTGC CTGGTTAGAT 
CAATACTCGG AGGATTTCTG GAGTCCTCCA GATCACGAAT GCCTGCAGAG TCTCATATCC 
TACTTACAAC TCAACTTCTC TGGATCGGAC CTGGAGAGAC GAGCTTTAAA CCTGCTGGAG 
CACTTCCAGC ATCGCCAACA GTGCGAGGTG GAGATTGAAG GTGACCTGTG CTCCTGCCCC 
TTTGCCACAC CAGAGGAGAG CAGTTTGGAG GATGAATTCT CCCCCTCACA TTTCATGTCC 
TTCAGTCCTT CTATAGTTGC TGAGCAGTTA ACAGTCATGG ATGCGGAGCT GTTTAAGAGG 
GTTGTTCCAT ATCATTGTTT AGGGGGCATA TGGTCCCAGA GAGATAAAAA GGGCAAAGAG 
CACCTGGCAC CAACCATCCG TGCTACTGTT TCTCAGTTCA ACAGTGTGAC CAACTGTGTG 
ATCGCCACCT GCCTGGACAA CAGGTCTCTC AGACCTTTCC AAAGAGCCAA GCGCATTGAA 
CATTGGATAG AAGTTGCCAG GAAATGCCGT ATCCTGAAGA GCTTCTCCTC TCTGAAAGCC 
ATCCTGTCTG CCTTGCAGAG TAATGCCATC CACAGGCTGA GAAGGGCCTG GGATGATGTT 
TCACGGGAAA GCTATCGAAC TTTCCAAGAG CTGTCTGAAA TCTTCTCAGA CGACAATAAT 
TACTCACTCA GCAGAGAGCT TCTCATTAAG GAGGGCACCT CTAAATCTGC CATAGTCGAG 
ATAAACCACA AAGGAGCCCA GAGGAGACAC CAGCAGCAAA GAGACATGGG TGTTGTGCAA 
GGAACGATTC CATACCTTGG CACTTTTCTG ACTGACCTCG TCATGTTGGA TACTGCTATG 
AAAGACCATC TGGAGGTCGG ACTGATTAAC TTTGAGAAGA GAAGAAAGGA ATTTGAAGTG 
ATCGCTCAGA TCAAGTTGCT GCAGCTGACC TGCAATTATT ACAACTTCAG CAGAAACCAG 
CGCTTTATTG ACTGGTTTAA GAGAGTGGAG AAACTCACAG AGACCGAGAG CTACTCTATG 
TCGTGTGATA TCGAGCCGCC GTCTGAGTCG TTGTGCAAAA AGAATGGAGG CATCATGAAG 
CGCATGAGCG AGGAGTCGGG CTACAGCAGC GCTGCGTCAC ACTCCAAGTC CTTTGAGCAG 
CCGCGATTAA GCCAGTTCAA AGACTGCTTT AAAGATGGAG CTGATTCCCT CAGCCTCACC 
TCTGCAGGAT CCAGTGGCTC AGATGCAGAC GAGAGTCTCA GCCTGCTCAA CTCTCCTGAA 
TCAGGCAGTC AGAGACTCTG GGAATCACCC ACCCCATCAT CTCTGGAGGC GTCTGGATCA 
GGCTTGTGTT TAGAGTCAGG CTGCAGCAGC TCCACATCTT CCTCCTCTTC CTCCTCGTCC 
TCAGTCTCAG CCTTCACAGG ACGGACCTTC AACTTTCACA AGCGCTCAGT CTCTGGCATC 
TCAGACTACT CGTCTCTCAG TCTGCCGCTC TACAACCAGC AGGCAAACGA CTGCTGCATC 
ATCAGAGTCA GCCTGGACTC AGAAAACGGC AACATGTACA AAAGTATCCT GGTCACTAGT 
CAGGACAAAA CCCCTGGGGT CATTAGGAAA GCCATGGCCA AACACAACCT GGACAATGAC 
AAATCTGAAG ACTATGAATT GTTACAGAGG GTCTCAAAGC ATAAAGAACT CAGAATCCCA 
GATAATGGAA ACGTTTTCTA CGCCATGAAC TCAACTGCCA ACTACGACTT CTTGTTGAGA 
AAGCGTGGCG TCCCAAAAAC CTGCCGCTCG AAAAGCTCCC CGAGCCTGAC TCTACCACGC 
ATGAAGCAGA AAGGCTTCAA AATCCCCAAG AGCTTCTTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001116789.1
CDS	1..2442
Translation	MVYIMGIQSDALGVKPTSLDELFESSTWRIKNIWDGVKLEIAAGDGCPVVLNSFTHLDPDLPMLESSVQEIGEEVEDGAIYTITLRKVQLHQTASKGQRWLGVETDSALSLYETCKGRTIKAGTLEKLVEYMVSAFKGKDYTYVTIFLCTYRAFATTKQVLDLLLNRYAKLPASGKTKVSAEDHTELKNTVSSILGAWLDQYSEDFWSPPDHECLQSLISYLQLNFSGSDLERRALNLLEHFQHRQQCEVEIEGDLCSCPFATPEESSLEDEFSPSHFMSFSPSIVAEQLTVMDAELFKRVVPYHCLGGIWSQRDKKGKEHLAPTIRATVSQFNSVTNCVIATCLDNRSLRPFQRAKRIEHWIEVARKCRILKSFSSLKAILSALQSNAIHRLRRAWDDVSRESYRTFQELSEIFSDDNNYSLSRELLIKEGTSKSAIVEINHKGAQRRHQQQRDMGVVQGTIPYLGTFLTDLVMLDTAMKDHLEVGLINFEKRRKEFEVIAQIKLLQLTCNYYNFSRNQRFIDWFKRVEKLTETESYSMSCDIEPPSESLCKKNGGIMKRMSEESGYSSAASHSKSFEQPRLSQFKDCFKDGADSLSLTSAGSSGSDADESLSLLNSPESGSQRLWESPTPSSLEASGSGLCLESGCSSSTSSSSSSSSSVSAFTGRTFNFHKRSVSGISDYSSLSLPLYNQQANDCCIIRVSLDSENGNMYKSILVTSQDKTPGVIRKAMAKHNLDNDKSEDYELLQRVSKHKELRIPDNGNVFYAMNSTANYDFLLRKRGVPKTCRSKSSPSLTLPRMKQKGFKIPKSFF

Target ORF information:

RefSeq Version	NM_001123317.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio si:ch211-251j10.3 (si:ch211-251j10.3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001123317.1

ORF Insert Sequence:

ATGGTCTATA TTATGGGGAT ACAGTCGGAC GCCCTGGGTG TTAAACCCAC GTCCCTGGAT 
GAGCTGTTTG AGTCCAGTAC ATGGAGAATT AAAAACATAT GGGATGGGGT GAAGCTGGAG 
ATCGCGGCCG GAGATGGATG TCCGGTGGTC TTAAATAGTT TTACGCATTT GGACCCAGAT 
CTACCTATGT TGGAGAGCTC AGTTCAGGAG ATTGGAGAGG AGGTGGAGGA CGGTGCCATC 
TATACCATTA CACTCCGAAA GGTGCAGCTC CACCAGACGG CCAGTAAGGG CCAGCGATGG 
CTGGGCGTGG AGACAGACTC TGCCCTCAGT CTGTACGAGA CGTGCAAGGG TCGAACCATT 
AAGGCTGGCA CACTGGAGAA ACTAGTGGAG TACATGGTCT CCGCCTTCAA AGGGAAAGAC 
TACACTTACG TCACTATTTT CCTCTGCACC TACAGAGCAT TTGCCACCAC CAAACAAGTG 
CTAGACCTGC TGCTTAACAG ATATGCCAAA CTGCCAGCCT CAGGCAAGAC TAAAGTCTCA 
GCAGAAGACC ACACAGAGCT TAAAAACACT GTGTCATCTA TCCTGGGTGC CTGGTTAGAT 
CAATACTCGG AGGATTTCTG GAGTCCTCCA GATCACGAAT GCCTGCAGAG TCTCATATCC 
TACTTACAAC TCAACTTCTC TGGATCGGAC CTGGAGAGAC GAGCTTTAAA CCTGCTGGAG 
CACTTCCAGC ATCGCCAACA GTGCGAGGTG GAGATTGAAG GTGACCTGTG CTCCTGCCCC 
TTTGCCACAC CAGAGGAGAG CAGTTTGGAG GATGAATTCT CCCCCTCACA TTTCATGTCC 
TTCAGTCCTT CTATAGTTGC TGAGCAGTTA ACAGTCATGG ATGCGGAGCT GTTTAAGAGG 
GTTGTTCCAT ATCATTGTTT AGGGGGCATA TGGTCCCAGA GAGATAAAAA GGGCAAAGAG 
CACCTGGCAC CAACCATCCG TGCTACTGTT TCTCAGTTCA ACAGTGTGAC CAACTGTGTG 
ATCGCCACCT GCCTGGACAA CAGGTCTCTC AGACCTTTCC AAAGAGCCAA GCGCATTGAA 
CATTGGATAG AAGTTGCCAG GAAATGCCGT ATCCTGAAGA GCTTCTCCTC TCTGAAAGCC 
ATCCTGTCTG CCTTGCAGAG TAATGCCATC CACAGGCTGA GAAGGGCCTG GGATGATGTT 
TCACGGGAAA GCTATCGAAC TTTCCAAGAG CTGTCTGAAA TCTTCTCAGA CGACAATAAT 
TACTCACTCA GCAGAGAGCT TCTCATTAAG GAGGGCACCT CTAAATCTGC CATAGTCGAG 
ATAAACCACA AAGGAGCCCA GAGGAGACAC CAGCAGCAAA GAGACATGGG TGTTGTGCAA 
GGAACGATTC CATACCTTGG CACTTTTCTG ACTGACCTCG TCATGTTGGA TACTGCTATG 
AAAGACCATC TGGAGGTCGG ACTGATTAAC TTTGAGAAGA GAAGAAAGGA ATTTGAAGTG 
ATCGCTCAGA TCAAGTTGCT GCAGCTGACC TGCAATTATT ACAACTTCAG CAGAAACCAG 
CGCTTTATTG ACTGGTTTAA GAGAGTGGAG AAACTCACAG AGACCGAGAG CTACTCTATG 
TCGTGTGATA TCGAGCCGCC GTCTGAGTCG TTGTGCAAAA AGAATGGAGG CATCATGAAG 
CGCATGAGCG AGGAGTCGGG CTACAGCAGC GCTGCGTCAC ACTCCAAGTC CTTTGAGCAG 
CCGCGATTAA GCCAGTTCAA AGACTGCTTT AAAGATGGAG CTGATTCCCT CAGCCTCACC 
TCTGCAGGAT CCAGTGGCTC AGATGCAGAC GAGAGTCTCA GCCTGCTCAA CTCTCCTGAA 
TCAGGCAGTC AGAGACTCTG GGAATCACCC ACCCCATCAT CTCTGGAGGC GTCTGGATCA 
GGCTTGTGTT TAGAGTCAGG CTGCAGCAGC TCCACATCTT CCTCCTCTTC CTCCTCGTCC 
TCAGTCTCAG CCTTCACAGG ACGGACCTTC AACTTTCACA AGCGCTCAGT CTCTGGCATC 
TCAGACTACT CGTCTCTCAG TCTGCCGCTC TACAACCAGC AGGCAAACGA CTGCTGCATC 
ATCAGAGTCA GCCTGGACTC AGAAAACGGC AACATGTACA AAAGTATCCT GGTCACTAGT 
CAGGACAAAA CCCCTGGGGT CATTAGGAAA GCCATGGCCA AACACAACCT GGACAATGAC 
AAATCTGAAG ACTATGAATT GTTACAGAGG GTCTCAAAGC ATAAAGAACT CAGAATCCCA 
GATAATGGAA ACGTTTTCTA CGCCATGAAC TCAACTGCCA ACTACGACTT CTTGTTGAGA 
AAGCGTGGCG TCCCAAAAAC CTGCCGCTCG AAAAGCTCCC CGAGCCTGAC TCTACCACGC 
ATGAAGCAGA AAGGCTTCAA AATCCCCAAG AGCTTCTTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

si:ch211-251j10.3 ( XM_005167182.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005167239.1 Danio rerio si:ch211-251j10.3 (si:ch211-251j10.3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa14382
Clone ID Related Accession (Same CDS sequence)	XM_005167182.4 , XM_005167182.3
Accession Version	XM_005167182.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2514bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	ral guanine nucleotide dissociation stimulator isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005167182.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCTATA TTATGGGGAT ACAGTCGGAC GCCCTGGGTG TTAAACCCAC GTCCCTGGAT 
GAGCTGTTTG AGTCCAGTAC ATGGAGAATT AAAAACATAT GGGATGGGGT GAAGCTGGAG 
ATCGCGGCCG GAGATGGATG TCCGGTGGTC TTAAATAGTT TTACGCATTT GGACCCAGAT 
CTACCTATGT TGGAGAGCTC AGTTCAGGAG ATTGGAGAGG AGGTGGAGGA CGGTGCCATC 
TATACCATTA CACTCCGAAA GGTGCAGCTC CACCAGACGG CCAGTAAGGG CCAGCGATGG 
CTGGGCGTGG AGACAGACTC TGCCCTCAGT CTGTACGAGA CGTGCAAGGG TCGAACCATT 
AAGGCTGGCA CACTGGAGAA ACTAGTGGAG TACATGGTCT CCGCCTTCAA AGGGAAAGAC 
TACACTTACG TCACTATTTT CCTCTGCACC TACAGAGCAT TTGCCACCAC CAAACAAGTG 
CTAGACCTGC TGCTTAACAG ATATGCCAAA CTGCCAGCCT CAGGCAAGAC TAAAGTCTCA 
GCAGAAGACC ACACAGAGCT TAAAAACACT GTGTCATCTA TCCTGGGTGC CTGGTTAGAT 
CAATACTCGG AGGATTTCTG GAGTCCTCCA GATCACGAAT GCCTGCAGAG TCTCATATCC 
TACTTACAAC TCAACTTCTC TGGATCGGAC CTGGAGAGAC GAGCTTTAAA CCTGCTGGAG 
CACTTCCAGC ATCGCCAACA GTGCGAGGTG GAGATTGAAG GTGACCTGTG CTCCTGCCCC 
TTTGCCACAC CAGAGGAGAG CAGTTTGGAG GATGAATTCT CCCCCTCACA TTTCATGTCC 
TTCAGTCCTT CTATAGTTGC TGAGCAGTTA ACAGTCATGG ATGCGGAGCT GTTTAAGAGG 
GTTGTTCCAT ATCATTGTTT AGGGGGCATA TGGTCCCAGA GAGATAAAAA GGGCAAAGAG 
CACCTGGCAC CAACCATCCG TGCTACTGTT TCTCAGTTCA ACAGTGTGAC CAACTGTGTG 
ATCGCCACCT GCCTGGACAA CAGGTCTCTC AGACCTTTCC AAAGAGCCAA GCGCATTGAA 
CATTGGATAG AAGTTGCCAG GAAATGCCGT ATCCTGAAGA GCTTCTCCTC TCTGAAAGCC 
ATCCTGTCTG CCTTGCAGAG TAATGCCATC CACAGGCTGA GAAGGGCCTG GGATGATGTT 
TCACGGGAAA GCTATCGAAC TTTCCAAGAG CTGTCTGAAA TCTTCTCAGA CGACAATAAT 
TACTCACTCA GCAGAGAGCT TCTCATTAAG GAGGGCACCT CTAAATCTGC CATAGTCGAG 
ATAAACCACA AAGGAGCCCA GAGGAGACAC CAGCAGCAAA GAGACATGGG TGTTGTGCAA 
GGAACGATTC CATACCTTGG CACTTTTCTG ACTGACCTCG TCATGTTGGA TACTGCTATG 
AAAGACCATC TGGAGGTCGG ACTGATTAAC TTTGAGAAGA GAAGAAAGGA ATTTGAAGTG 
ATCGCTCAGA TCAAGTTGCT GCAGCTGACC TGCAATTATT ACAACTTCAG CAGAAACCAG 
CGCTTTATTG ACTGGTTTAA GAGAGTGGAG AAACTCACAG AGACCGAGAG CTACTCTATG 
TCGTGTGATA TCGAGCCGCC GTCTGAGTCG TTGTGCAAAA AGAATGGAGG CATCATGAAG 
CGCATGAGCG AGGAGTCGGG CTACAGCAGC GCTGCGTCAC ACTCCAAGTC CTTTGAGCAG 
CCGCGATTAA GCCAGTTCAA AGACTGCTTT AAAGATGGAG CTGATTCCCT CAGCCTCACC 
TCTGCAGGAT CCAGTGGCTC AGATGCAGAC GAGAGTCTCA GCCTGCTCAA CTCTCCTGAA 
TCAGGCAGTC AGAGAACATC CACACCAACT GTGAAACACT CATCAACCTC AGAAACAGGA 
GAGCTCCCGT CTGATTCCTC AACCAAGCTC TGGGAATCAC CCACCCCATC ATCTCTGGAG 
GCGTCTGGAT CAGGCTTGTG TTTAGAGTCA GGCTGCAGCA GCTCCACATC TTCCTCCTCT 
TCCTCCTCGT CCTCAGTCTC AGCCTTCACA GGACGGACCT TCAACTTTCA CAAGCGCTCA 
GTCTCTGGCA TCTCAGACTA CTCGTCTCTC AGTCTGCCGC TCTACAACCA GCAGGCAAAC 
GACTGCTGCA TCATCAGAGT CAGCCTGGAC TCAGAAAACG GCAACATGTA CAAAAGTATC 
CTGGTCACTA GTCAGGACAA AACCCCTGGG GTCATTAGGA AAGCCATGGC CAAACACAAC 
CTGGACAATG ACAAATCTGA AGACTATGAA TTGTTACAGA GGGTCTCAAA GCATAAAGAA 
CTCAGAATCC CAGATAATGG AAACGTTTTC TACGCCATGA ACTCAACTGC CAACTACGAC 
TTCTTGTTGA GAAAGCGTGG CGTCCCAAAA ACCTGCCGCT CGAAAAGCTC CCCGAGCCTG 
ACTCTACCAC GCATGAAGCA GAAAGGCTTC AAAATCCCCA AGAGCTTCTT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005167239.1
CDS	139..2652
Translation	MVYIMGIQSDALGVKPTSLDELFESSTWRIKNIWDGVKLEIAAGDGCPVVLNSFTHLDPDLPMLESSVQEIGEEVEDGAIYTITLRKVQLHQTASKGQRWLGVETDSALSLYETCKGRTIKAGTLEKLVEYMVSAFKGKDYTYVTIFLCTYRAFATTKQVLDLLLNRYAKLPASGKTKVSAEDHTELKNTVSSILGAWLDQYSEDFWSPPDHECLQSLISYLQLNFSGSDLERRALNLLEHFQHRQQCEVEIEGDLCSCPFATPEESSLEDEFSPSHFMSFSPSIVAEQLTVMDAELFKRVVPYHCLGGIWSQRDKKGKEHLAPTIRATVSQFNSVTNCVIATCLDNRSLRPFQRAKRIEHWIEVARKCRILKSFSSLKAILSALQSNAIHRLRRAWDDVSRESYRTFQELSEIFSDDNNYSLSRELLIKEGTSKSAIVEINHKGAQRRHQQQRDMGVVQGTIPYLGTFLTDLVMLDTAMKDHLEVGLINFEKRRKEFEVIAQIKLLQLTCNYYNFSRNQRFIDWFKRVEKLTETESYSMSCDIEPPSESLCKKNGGIMKRMSEESGYSSAASHSKSFEQPRLSQFKDCFKDGADSLSLTSAGSSGSDADESLSLLNSPESGSQRTSTPTVKHSSTSETGELPSDSSTKLWESPTPSSLEASGSGLCLESGCSSSTSSSSSSSSSVSAFTGRTFNFHKRSVSGISDYSSLSLPLYNQQANDCCIIRVSLDSENGNMYKSILVTSQDKTPGVIRKAMAKHNLDNDKSEDYELLQRVSKHKELRIPDNGNVFYAMNSTANYDFLLRKRGVPKTCRSKSSPSLTLPRMKQKGFKIPKSFF

Target ORF information:

RefSeq Version	XM_005167182.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio si:ch211-251j10.3 (si:ch211-251j10.3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005167182.4

ORF Insert Sequence:

ATGGTCTATA TTATGGGGAT ACAGTCGGAC GCCCTGGGTG TTAAACCCAC GTCCCTGGAT 
GAGCTGTTTG AGTCCAGTAC ATGGAGAATT AAAAACATAT GGGATGGGGT GAAGCTGGAG 
ATCGCGGCCG GAGATGGATG TCCGGTGGTC TTAAATAGTT TTACGCATTT GGACCCAGAT 
CTACCTATGT TGGAGAGCTC AGTTCAGGAG ATTGGAGAGG AGGTGGAGGA CGGTGCCATC 
TATACCATTA CACTCCGAAA GGTGCAGCTC CACCAGACGG CCAGTAAGGG CCAGCGATGG 
CTGGGCGTGG AGACAGACTC TGCCCTCAGT CTGTACGAGA CGTGCAAGGG TCGAACCATT 
AAGGCTGGCA CACTGGAGAA ACTAGTGGAG TACATGGTCT CCGCCTTCAA AGGGAAAGAC 
TACACTTACG TCACTATTTT CCTCTGCACC TACAGAGCAT TTGCCACCAC CAAACAAGTG 
CTAGACCTGC TGCTTAACAG ATATGCCAAA CTGCCAGCCT CAGGCAAGAC TAAAGTCTCA 
GCAGAAGACC ACACAGAGCT TAAAAACACT GTGTCATCTA TCCTGGGTGC CTGGTTAGAT 
CAATACTCGG AGGATTTCTG GAGTCCTCCA GATCACGAAT GCCTGCAGAG TCTCATATCC 
TACTTACAAC TCAACTTCTC TGGATCGGAC CTGGAGAGAC GAGCTTTAAA CCTGCTGGAG 
CACTTCCAGC ATCGCCAACA GTGCGAGGTG GAGATTGAAG GTGACCTGTG CTCCTGCCCC 
TTTGCCACAC CAGAGGAGAG CAGTTTGGAG GATGAATTCT CCCCCTCACA TTTCATGTCC 
TTCAGTCCTT CTATAGTTGC TGAGCAGTTA ACAGTCATGG ATGCGGAGCT GTTTAAGAGG 
GTTGTTCCAT ATCATTGTTT AGGGGGCATA TGGTCCCAGA GAGATAAAAA GGGCAAAGAG 
CACCTGGCAC CAACCATCCG TGCTACTGTT TCTCAGTTCA ACAGTGTGAC CAACTGTGTG 
ATCGCCACCT GCCTGGACAA CAGGTCTCTC AGACCTTTCC AAAGAGCCAA GCGCATTGAA 
CATTGGATAG AAGTTGCCAG GAAATGCCGT ATCCTGAAGA GCTTCTCCTC TCTGAAAGCC 
ATCCTGTCTG CCTTGCAGAG TAATGCCATC CACAGGCTGA GAAGGGCCTG GGATGATGTT 
TCACGGGAAA GCTATCGAAC TTTCCAAGAG CTGTCTGAAA TCTTCTCAGA CGACAATAAT 
TACTCACTCA GCAGAGAGCT TCTCATTAAG GAGGGCACCT CTAAATCTGC CATAGTCGAG 
ATAAACCACA AAGGAGCCCA GAGGAGACAC CAGCAGCAAA GAGACATGGG TGTTGTGCAA 
GGAACGATTC CATACCTTGG CACTTTTCTG ACTGACCTCG TCATGTTGGA TACTGCTATG 
AAAGACCATC TGGAGGTCGG ACTGATTAAC TTTGAGAAGA GAAGAAAGGA ATTTGAAGTG 
ATCGCTCAGA TCAAGTTGCT GCAGCTGACC TGCAATTATT ACAACTTCAG CAGAAACCAG 
CGCTTTATTG ACTGGTTTAA GAGAGTGGAG AAACTCACAG AGACCGAGAG CTACTCTATG 
TCGTGTGATA TCGAGCCGCC GTCTGAGTCG TTGTGCAAAA AGAATGGAGG CATCATGAAG 
CGCATGAGCG AGGAGTCGGG CTACAGCAGC GCTGCGTCAC ACTCCAAGTC CTTTGAGCAG 
CCGCGATTAA GCCAGTTCAA AGACTGCTTT AAAGATGGAG CTGATTCCCT CAGCCTCACC 
TCTGCAGGAT CCAGTGGCTC AGATGCAGAC GAGAGTCTCA GCCTGCTCAA CTCTCCTGAA 
TCAGGCAGTC AGAGAACATC CACACCAACT GTGAAACACT CATCAACCTC AGAAACAGGA 
GAGCTCCCGT CTGATTCCTC AACCAAGCTC TGGGAATCAC CCACCCCATC ATCTCTGGAG 
GCGTCTGGAT CAGGCTTGTG TTTAGAGTCA GGCTGCAGCA GCTCCACATC TTCCTCCTCT 
TCCTCCTCGT CCTCAGTCTC AGCCTTCACA GGACGGACCT TCAACTTTCA CAAGCGCTCA 
GTCTCTGGCA TCTCAGACTA CTCGTCTCTC AGTCTGCCGC TCTACAACCA GCAGGCAAAC 
GACTGCTGCA TCATCAGAGT CAGCCTGGAC TCAGAAAACG GCAACATGTA CAAAAGTATC 
CTGGTCACTA GTCAGGACAA AACCCCTGGG GTCATTAGGA AAGCCATGGC CAAACACAAC 
CTGGACAATG ACAAATCTGA AGACTATGAA TTGTTACAGA GGGTCTCAAA GCATAAAGAA 
CTCAGAATCC CAGATAATGG AAACGTTTTC TACGCCATGA ACTCAACTGC CAACTACGAC 
TTCTTGTTGA GAAAGCGTGG CGTCCCAAAA ACCTGCCGCT CGAAAAGCTC CCCGAGCCTG 
ACTCTACCAC GCATGAAGCA GAAAGGCTTC AAAATCCCCA AGAGCTTCTT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

si:ch211-251j10.3 ( XM_005167182.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005167239.1 Danio rerio si:ch211-251j10.3 (si:ch211-251j10.3), transcript variant X1, mRNA.

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CloneID	ODa14382
Clone ID Related Accession (Same CDS sequence)	XM_005167182.4 , XM_005167182.3
Accession Version	XM_005167182.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2514bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	ral guanine nucleotide dissociation stimulator isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005167182.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCTATA TTATGGGGAT ACAGTCGGAC GCCCTGGGTG TTAAACCCAC GTCCCTGGAT 
GAGCTGTTTG AGTCCAGTAC ATGGAGAATT AAAAACATAT GGGATGGGGT GAAGCTGGAG 
ATCGCGGCCG GAGATGGATG TCCGGTGGTC TTAAATAGTT TTACGCATTT GGACCCAGAT 
CTACCTATGT TGGAGAGCTC AGTTCAGGAG ATTGGAGAGG AGGTGGAGGA CGGTGCCATC 
TATACCATTA CACTCCGAAA GGTGCAGCTC CACCAGACGG CCAGTAAGGG CCAGCGATGG 
CTGGGCGTGG AGACAGACTC TGCCCTCAGT CTGTACGAGA CGTGCAAGGG TCGAACCATT 
AAGGCTGGCA CACTGGAGAA ACTAGTGGAG TACATGGTCT CCGCCTTCAA AGGGAAAGAC 
TACACTTACG TCACTATTTT CCTCTGCACC TACAGAGCAT TTGCCACCAC CAAACAAGTG 
CTAGACCTGC TGCTTAACAG ATATGCCAAA CTGCCAGCCT CAGGCAAGAC TAAAGTCTCA 
GCAGAAGACC ACACAGAGCT TAAAAACACT GTGTCATCTA TCCTGGGTGC CTGGTTAGAT 
CAATACTCGG AGGATTTCTG GAGTCCTCCA GATCACGAAT GCCTGCAGAG TCTCATATCC 
TACTTACAAC TCAACTTCTC TGGATCGGAC CTGGAGAGAC GAGCTTTAAA CCTGCTGGAG 
CACTTCCAGC ATCGCCAACA GTGCGAGGTG GAGATTGAAG GTGACCTGTG CTCCTGCCCC 
TTTGCCACAC CAGAGGAGAG CAGTTTGGAG GATGAATTCT CCCCCTCACA TTTCATGTCC 
TTCAGTCCTT CTATAGTTGC TGAGCAGTTA ACAGTCATGG ATGCGGAGCT GTTTAAGAGG 
GTTGTTCCAT ATCATTGTTT AGGGGGCATA TGGTCCCAGA GAGATAAAAA GGGCAAAGAG 
CACCTGGCAC CAACCATCCG TGCTACTGTT TCTCAGTTCA ACAGTGTGAC CAACTGTGTG 
ATCGCCACCT GCCTGGACAA CAGGTCTCTC AGACCTTTCC AAAGAGCCAA GCGCATTGAA 
CATTGGATAG AAGTTGCCAG GAAATGCCGT ATCCTGAAGA GCTTCTCCTC TCTGAAAGCC 
ATCCTGTCTG CCTTGCAGAG TAATGCCATC CACAGGCTGA GAAGGGCCTG GGATGATGTT 
TCACGGGAAA GCTATCGAAC TTTCCAAGAG CTGTCTGAAA TCTTCTCAGA CGACAATAAT 
TACTCACTCA GCAGAGAGCT TCTCATTAAG GAGGGCACCT CTAAATCTGC CATAGTCGAG 
ATAAACCACA AAGGAGCCCA GAGGAGACAC CAGCAGCAAA GAGACATGGG TGTTGTGCAA 
GGAACGATTC CATACCTTGG CACTTTTCTG ACTGACCTCG TCATGTTGGA TACTGCTATG 
AAAGACCATC TGGAGGTCGG ACTGATTAAC TTTGAGAAGA GAAGAAAGGA ATTTGAAGTG 
ATCGCTCAGA TCAAGTTGCT GCAGCTGACC TGCAATTATT ACAACTTCAG CAGAAACCAG 
CGCTTTATTG ACTGGTTTAA GAGAGTGGAG AAACTCACAG AGACCGAGAG CTACTCTATG 
TCGTGTGATA TCGAGCCGCC GTCTGAGTCG TTGTGCAAAA AGAATGGAGG CATCATGAAG 
CGCATGAGCG AGGAGTCGGG CTACAGCAGC GCTGCGTCAC ACTCCAAGTC CTTTGAGCAG 
CCGCGATTAA GCCAGTTCAA AGACTGCTTT AAAGATGGAG CTGATTCCCT CAGCCTCACC 
TCTGCAGGAT CCAGTGGCTC AGATGCAGAC GAGAGTCTCA GCCTGCTCAA CTCTCCTGAA 
TCAGGCAGTC AGAGAACATC CACACCAACT GTGAAACACT CATCAACCTC AGAAACAGGA 
GAGCTCCCGT CTGATTCCTC AACCAAGCTC TGGGAATCAC CCACCCCATC ATCTCTGGAG 
GCGTCTGGAT CAGGCTTGTG TTTAGAGTCA GGCTGCAGCA GCTCCACATC TTCCTCCTCT 
TCCTCCTCGT CCTCAGTCTC AGCCTTCACA GGACGGACCT TCAACTTTCA CAAGCGCTCA 
GTCTCTGGCA TCTCAGACTA CTCGTCTCTC AGTCTGCCGC TCTACAACCA GCAGGCAAAC 
GACTGCTGCA TCATCAGAGT CAGCCTGGAC TCAGAAAACG GCAACATGTA CAAAAGTATC 
CTGGTCACTA GTCAGGACAA AACCCCTGGG GTCATTAGGA AAGCCATGGC CAAACACAAC 
CTGGACAATG ACAAATCTGA AGACTATGAA TTGTTACAGA GGGTCTCAAA GCATAAAGAA 
CTCAGAATCC CAGATAATGG AAACGTTTTC TACGCCATGA ACTCAACTGC CAACTACGAC 
TTCTTGTTGA GAAAGCGTGG CGTCCCAAAA ACCTGCCGCT CGAAAAGCTC CCCGAGCCTG 
ACTCTACCAC GCATGAAGCA GAAAGGCTTC AAAATCCCCA AGAGCTTCTT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005167239.1
CDS	158..2671
Translation	MVYIMGIQSDALGVKPTSLDELFESSTWRIKNIWDGVKLEIAAGDGCPVVLNSFTHLDPDLPMLESSVQEIGEEVEDGAIYTITLRKVQLHQTASKGQRWLGVETDSALSLYETCKGRTIKAGTLEKLVEYMVSAFKGKDYTYVTIFLCTYRAFATTKQVLDLLLNRYAKLPASGKTKVSAEDHTELKNTVSSILGAWLDQYSEDFWSPPDHECLQSLISYLQLNFSGSDLERRALNLLEHFQHRQQCEVEIEGDLCSCPFATPEESSLEDEFSPSHFMSFSPSIVAEQLTVMDAELFKRVVPYHCLGGIWSQRDKKGKEHLAPTIRATVSQFNSVTNCVIATCLDNRSLRPFQRAKRIEHWIEVARKCRILKSFSSLKAILSALQSNAIHRLRRAWDDVSRESYRTFQELSEIFSDDNNYSLSRELLIKEGTSKSAIVEINHKGAQRRHQQQRDMGVVQGTIPYLGTFLTDLVMLDTAMKDHLEVGLINFEKRRKEFEVIAQIKLLQLTCNYYNFSRNQRFIDWFKRVEKLTETESYSMSCDIEPPSESLCKKNGGIMKRMSEESGYSSAASHSKSFEQPRLSQFKDCFKDGADSLSLTSAGSSGSDADESLSLLNSPESGSQRTSTPTVKHSSTSETGELPSDSSTKLWESPTPSSLEASGSGLCLESGCSSSTSSSSSSSSSVSAFTGRTFNFHKRSVSGISDYSSLSLPLYNQQANDCCIIRVSLDSENGNMYKSILVTSQDKTPGVIRKAMAKHNLDNDKSEDYELLQRVSKHKELRIPDNGNVFYAMNSTANYDFLLRKRGVPKTCRSKSSPSLTLPRMKQKGFKIPKSFF

Target ORF information:

RefSeq Version	XM_005167182.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio si:ch211-251j10.3 (si:ch211-251j10.3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005167182.3

ORF Insert Sequence:

ATGGTCTATA TTATGGGGAT ACAGTCGGAC GCCCTGGGTG TTAAACCCAC GTCCCTGGAT 
GAGCTGTTTG AGTCCAGTAC ATGGAGAATT AAAAACATAT GGGATGGGGT GAAGCTGGAG 
ATCGCGGCCG GAGATGGATG TCCGGTGGTC TTAAATAGTT TTACGCATTT GGACCCAGAT 
CTACCTATGT TGGAGAGCTC AGTTCAGGAG ATTGGAGAGG AGGTGGAGGA CGGTGCCATC 
TATACCATTA CACTCCGAAA GGTGCAGCTC CACCAGACGG CCAGTAAGGG CCAGCGATGG 
CTGGGCGTGG AGACAGACTC TGCCCTCAGT CTGTACGAGA CGTGCAAGGG TCGAACCATT 
AAGGCTGGCA CACTGGAGAA ACTAGTGGAG TACATGGTCT CCGCCTTCAA AGGGAAAGAC 
TACACTTACG TCACTATTTT CCTCTGCACC TACAGAGCAT TTGCCACCAC CAAACAAGTG 
CTAGACCTGC TGCTTAACAG ATATGCCAAA CTGCCAGCCT CAGGCAAGAC TAAAGTCTCA 
GCAGAAGACC ACACAGAGCT TAAAAACACT GTGTCATCTA TCCTGGGTGC CTGGTTAGAT 
CAATACTCGG AGGATTTCTG GAGTCCTCCA GATCACGAAT GCCTGCAGAG TCTCATATCC 
TACTTACAAC TCAACTTCTC TGGATCGGAC CTGGAGAGAC GAGCTTTAAA CCTGCTGGAG 
CACTTCCAGC ATCGCCAACA GTGCGAGGTG GAGATTGAAG GTGACCTGTG CTCCTGCCCC 
TTTGCCACAC CAGAGGAGAG CAGTTTGGAG GATGAATTCT CCCCCTCACA TTTCATGTCC 
TTCAGTCCTT CTATAGTTGC TGAGCAGTTA ACAGTCATGG ATGCGGAGCT GTTTAAGAGG 
GTTGTTCCAT ATCATTGTTT AGGGGGCATA TGGTCCCAGA GAGATAAAAA GGGCAAAGAG 
CACCTGGCAC CAACCATCCG TGCTACTGTT TCTCAGTTCA ACAGTGTGAC CAACTGTGTG 
ATCGCCACCT GCCTGGACAA CAGGTCTCTC AGACCTTTCC AAAGAGCCAA GCGCATTGAA 
CATTGGATAG AAGTTGCCAG GAAATGCCGT ATCCTGAAGA GCTTCTCCTC TCTGAAAGCC 
ATCCTGTCTG CCTTGCAGAG TAATGCCATC CACAGGCTGA GAAGGGCCTG GGATGATGTT 
TCACGGGAAA GCTATCGAAC TTTCCAAGAG CTGTCTGAAA TCTTCTCAGA CGACAATAAT 
TACTCACTCA GCAGAGAGCT TCTCATTAAG GAGGGCACCT CTAAATCTGC CATAGTCGAG 
ATAAACCACA AAGGAGCCCA GAGGAGACAC CAGCAGCAAA GAGACATGGG TGTTGTGCAA 
GGAACGATTC CATACCTTGG CACTTTTCTG ACTGACCTCG TCATGTTGGA TACTGCTATG 
AAAGACCATC TGGAGGTCGG ACTGATTAAC TTTGAGAAGA GAAGAAAGGA ATTTGAAGTG 
ATCGCTCAGA TCAAGTTGCT GCAGCTGACC TGCAATTATT ACAACTTCAG CAGAAACCAG 
CGCTTTATTG ACTGGTTTAA GAGAGTGGAG AAACTCACAG AGACCGAGAG CTACTCTATG 
TCGTGTGATA TCGAGCCGCC GTCTGAGTCG TTGTGCAAAA AGAATGGAGG CATCATGAAG 
CGCATGAGCG AGGAGTCGGG CTACAGCAGC GCTGCGTCAC ACTCCAAGTC CTTTGAGCAG 
CCGCGATTAA GCCAGTTCAA AGACTGCTTT AAAGATGGAG CTGATTCCCT CAGCCTCACC 
TCTGCAGGAT CCAGTGGCTC AGATGCAGAC GAGAGTCTCA GCCTGCTCAA CTCTCCTGAA 
TCAGGCAGTC AGAGAACATC CACACCAACT GTGAAACACT CATCAACCTC AGAAACAGGA 
GAGCTCCCGT CTGATTCCTC AACCAAGCTC TGGGAATCAC CCACCCCATC ATCTCTGGAG 
GCGTCTGGAT CAGGCTTGTG TTTAGAGTCA GGCTGCAGCA GCTCCACATC TTCCTCCTCT 
TCCTCCTCGT CCTCAGTCTC AGCCTTCACA GGACGGACCT TCAACTTTCA CAAGCGCTCA 
GTCTCTGGCA TCTCAGACTA CTCGTCTCTC AGTCTGCCGC TCTACAACCA GCAGGCAAAC 
GACTGCTGCA TCATCAGAGT CAGCCTGGAC TCAGAAAACG GCAACATGTA CAAAAGTATC 
CTGGTCACTA GTCAGGACAA AACCCCTGGG GTCATTAGGA AAGCCATGGC CAAACACAAC 
CTGGACAATG ACAAATCTGA AGACTATGAA TTGTTACAGA GGGTCTCAAA GCATAAAGAA 
CTCAGAATCC CAGATAATGG AAACGTTTTC TACGCCATGA ACTCAACTGC CAACTACGAC 
TTCTTGTTGA GAAAGCGTGG CGTCCCAAAA ACCTGCCGCT CGAAAAGCTC CCCGAGCCTG 
ACTCTACCAC GCATGAAGCA GAAAGGCTTC AAAATCCCCA AGAGCTTCTT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

si:ch211-251j10.3 ( XM_009304057.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009302332.1 Danio rerio si:ch211-251j10.3 (si:ch211-251j10.3), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa47376
Clone ID Related Accession (Same CDS sequence)	XM_009304057.2 , XM_009304057.3
Accession Version	XM_009304057.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2499bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	ral guanine nucleotide dissociation stimulator isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009304057.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAGCAG ACAAGGGGGA GCAGCATTCC CCTAATATGA AGCAGGCGCC TGGACAGCAG 
CCTACACTCT CTCCATCGGG TAAGATGGAG CCCAAATCTC TGTTCAGCCT GCATAAAGCT 
CTGGCTCAAC CTGTGAAGAT GTGCATGTTT GATTTCCCCG TCTCCATCCT GGACGATCTG 
AGCTCAGTTC AGGAGATTGG AGAGGAGGTG GAGGACGGTG CCATCTATAC CATTACACTC 
CGAAAGGTGC AGCTCCACCA GACGGCCAGT AAGGGCCAGC GATGGCTGGG CGTGGAGACA 
GACTCTGCCC TCAGTCTGTA CGAGACGTGC AAGGGTCGAA CCATTAAGGC TGGCACACTG 
GAGAAACTAG TGGAGTACAT GGTCTCCGCC TTCAAAGGGA AAGACTACAC TTACGTCACT 
ATTTTCCTCT GCACCTACAG AGCATTTGCC ACCACCAAAC AAGTGCTAGA CCTGCTGCTT 
AACAGATATG CCAAACTGCC AGCCTCAGGC AAGACTAAAG TCTCAGCAGA AGACCACACA 
GAGCTTAAAA ACACTGTGTC ATCTATCCTG GGTGCCTGGT TAGATCAATA CTCGGAGGAT 
TTCTGGAGTC CTCCAGATCA CGAATGCCTG CAGAGTCTCA TATCCTACTT ACAACTCAAC 
TTCTCTGGAT CGGACCTGGA GAGACGAGCT TTAAACCTGC TGGAGCACTT CCAGCATCGC 
CAACAGTGCG AGGTGGAGAT TGAAGGTGAC CTGTGCTCCT GCCCCTTTGC CACACCAGAG 
GAGAGCAGTT TGGAGGATGA ATTCTCCCCC TCACATTTCA TGTCCTTCAG TCCTTCTATA 
GTTGCTGAGC AGTTAACAGT CATGGATGCG GAGCTGTTTA AGAGGGTTGT TCCATATCAT 
TGTTTAGGGG GCATATGGTC CCAGAGAGAT AAAAAGGGCA AAGAGCACCT GGCACCAACC 
ATCCGTGCTA CTGTTTCTCA GTTCAACAGT GTGACCAACT GTGTGATCGC CACCTGCCTG 
GACAACAGGT CTCTCAGACC TTTCCAAAGA GCCAAGCGCA TTGAACATTG GATAGAAGTT 
GCCAGGAAAT GCCGTATCCT GAAGAGCTTC TCCTCTCTGA AAGCCATCCT GTCTGCCTTG 
CAGAGTAATG CCATCCACAG GCTGAGAAGG GCCTGGGATG ATGTTTCACG GGAAAGCTAT 
CGAACTTTCC AAGAGCTGTC TGAAATCTTC TCAGACGACA ATAATTACTC ACTCAGCAGA 
GAGCTTCTCA TTAAGGAGGG CACCTCTAAA TCTGCCATAG TCGAGATAAA CCACAAAGGA 
GCCCAGAGGA GACACCAGCA GCAAAGAGAC ATGGGTGTTG TGCAAGGAAC GATTCCATAC 
CTTGGCACTT TTCTGACTGA CCTCGTCATG TTGGATACTG CTATGAAAGA CCATCTGGAG 
GTCGGACTGA TTAACTTTGA GAAGAGAAGA AAGGAATTTG AAGTGATCGC TCAGATCAAG 
TTGCTGCAGC TGACCTGCAA TTATTACAAC TTCAGCAGAA ACCAGCGCTT TATTGACTGG 
TTTAAGAGAG TGGAGAAACT CACAGAGACC GAGAGCTACT CTATGTCGTG TGATATCGAG 
CCGCCGTCTG AGTCGTTGTG CAAAAAGAAT GGAGGCATCA TGAAGCGCAT GAGCGAGGAG 
TCGGGCTACA GCAGCGCTGC GTCACACTCC AAGTCCTTTG AGCAGCCGCG ATTAAGCCAG 
TTCAAAGACT GCTTTAAAGA TGGAGCTGAT TCCCTCAGCC TCACCTCTGC AGGATCCAGT 
GGCTCAGATG CAGACGAGAG TCTCAGCCTG CTCAACTCTC CTGAATCAGG CAGTCAGAGA 
ACATCCACAC CAACTGTGAA ACACTCATCA ACCTCAGAAA CAGGAGAGCT CCCGTCTGAT 
TCCTCAACCA AGCTCTGGGA ATCACCCACC CCATCATCTC TGGAGGCGTC TGGATCAGGC 
TTGTGTTTAG AGTCAGGCTG CAGCAGCTCC ACATCTTCCT CCTCTTCCTC CTCGTCCTCA 
GTCTCAGCCT TCACAGGACG GACCTTCAAC TTTCACAAGC GCTCAGTCTC TGGCATCTCA 
GACTACTCGT CTCTCAGTCT GCCGCTCTAC AACCAGCAGG CAAACGACTG CTGCATCATC 
AGAGTCAGCC TGGACTCAGA AAACGGCAAC ATGTACAAAA GTATCCTGGT CACTAGTCAG 
GACAAAACCC CTGGGGTCAT TAGGAAAGCC ATGGCCAAAC ACAACCTGGA CAATGACAAA 
TCTGAAGACT ATGAATTGTT ACAGAGGGTC TCAAAGCATA AAGAACTCAG AATCCCAGAT 
AATGGAAACG TTTTCTACGC CATGAACTCA ACTGCCAACT ACGACTTCTT GTTGAGAAAG 
CGTGGCGTCC CAAAAACCTG CCGCTCGAAA AGCTCCCCGA GCCTGACTCT ACCACGCATG 
AAGCAGAAAG GCTTCAAAAT CCCCAAGAGC TTCTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009302332.1
CDS	192..2690
Translation	MRADKGEQHSPNMKQAPGQQPTLSPSGKMEPKSLFSLHKALAQPVKMCMFDFPVSILDDLSSVQEIGEEVEDGAIYTITLRKVQLHQTASKGQRWLGVETDSALSLYETCKGRTIKAGTLEKLVEYMVSAFKGKDYTYVTIFLCTYRAFATTKQVLDLLLNRYAKLPASGKTKVSAEDHTELKNTVSSILGAWLDQYSEDFWSPPDHECLQSLISYLQLNFSGSDLERRALNLLEHFQHRQQCEVEIEGDLCSCPFATPEESSLEDEFSPSHFMSFSPSIVAEQLTVMDAELFKRVVPYHCLGGIWSQRDKKGKEHLAPTIRATVSQFNSVTNCVIATCLDNRSLRPFQRAKRIEHWIEVARKCRILKSFSSLKAILSALQSNAIHRLRRAWDDVSRESYRTFQELSEIFSDDNNYSLSRELLIKEGTSKSAIVEINHKGAQRRHQQQRDMGVVQGTIPYLGTFLTDLVMLDTAMKDHLEVGLINFEKRRKEFEVIAQIKLLQLTCNYYNFSRNQRFIDWFKRVEKLTETESYSMSCDIEPPSESLCKKNGGIMKRMSEESGYSSAASHSKSFEQPRLSQFKDCFKDGADSLSLTSAGSSGSDADESLSLLNSPESGSQRTSTPTVKHSSTSETGELPSDSSTKLWESPTPSSLEASGSGLCLESGCSSSTSSSSSSSSSVSAFTGRTFNFHKRSVSGISDYSSLSLPLYNQQANDCCIIRVSLDSENGNMYKSILVTSQDKTPGVIRKAMAKHNLDNDKSEDYELLQRVSKHKELRIPDNGNVFYAMNSTANYDFLLRKRGVPKTCRSKSSPSLTLPRMKQKGFKIPKSFF

Target ORF information:

RefSeq Version	XM_009304057.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio si:ch211-251j10.3 (si:ch211-251j10.3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009304057.2

ORF Insert Sequence:

ATGAGAGCAG ACAAGGGGGA GCAGCATTCC CCTAATATGA AGCAGGCGCC TGGACAGCAG 
CCTACACTCT CTCCATCGGG TAAGATGGAG CCCAAATCTC TGTTCAGCCT GCATAAAGCT 
CTGGCTCAAC CTGTGAAGAT GTGCATGTTT GATTTCCCCG TCTCCATCCT GGACGATCTG 
AGCTCAGTTC AGGAGATTGG AGAGGAGGTG GAGGACGGTG CCATCTATAC CATTACACTC 
CGAAAGGTGC AGCTCCACCA GACGGCCAGT AAGGGCCAGC GATGGCTGGG CGTGGAGACA 
GACTCTGCCC TCAGTCTGTA CGAGACGTGC AAGGGTCGAA CCATTAAGGC TGGCACACTG 
GAGAAACTAG TGGAGTACAT GGTCTCCGCC TTCAAAGGGA AAGACTACAC TTACGTCACT 
ATTTTCCTCT GCACCTACAG AGCATTTGCC ACCACCAAAC AAGTGCTAGA CCTGCTGCTT 
AACAGATATG CCAAACTGCC AGCCTCAGGC AAGACTAAAG TCTCAGCAGA AGACCACACA 
GAGCTTAAAA ACACTGTGTC ATCTATCCTG GGTGCCTGGT TAGATCAATA CTCGGAGGAT 
TTCTGGAGTC CTCCAGATCA CGAATGCCTG CAGAGTCTCA TATCCTACTT ACAACTCAAC 
TTCTCTGGAT CGGACCTGGA GAGACGAGCT TTAAACCTGC TGGAGCACTT CCAGCATCGC 
CAACAGTGCG AGGTGGAGAT TGAAGGTGAC CTGTGCTCCT GCCCCTTTGC CACACCAGAG 
GAGAGCAGTT TGGAGGATGA ATTCTCCCCC TCACATTTCA TGTCCTTCAG TCCTTCTATA 
GTTGCTGAGC AGTTAACAGT CATGGATGCG GAGCTGTTTA AGAGGGTTGT TCCATATCAT 
TGTTTAGGGG GCATATGGTC CCAGAGAGAT AAAAAGGGCA AAGAGCACCT GGCACCAACC 
ATCCGTGCTA CTGTTTCTCA GTTCAACAGT GTGACCAACT GTGTGATCGC CACCTGCCTG 
GACAACAGGT CTCTCAGACC TTTCCAAAGA GCCAAGCGCA TTGAACATTG GATAGAAGTT 
GCCAGGAAAT GCCGTATCCT GAAGAGCTTC TCCTCTCTGA AAGCCATCCT GTCTGCCTTG 
CAGAGTAATG CCATCCACAG GCTGAGAAGG GCCTGGGATG ATGTTTCACG GGAAAGCTAT 
CGAACTTTCC AAGAGCTGTC TGAAATCTTC TCAGACGACA ATAATTACTC ACTCAGCAGA 
GAGCTTCTCA TTAAGGAGGG CACCTCTAAA TCTGCCATAG TCGAGATAAA CCACAAAGGA 
GCCCAGAGGA GACACCAGCA GCAAAGAGAC ATGGGTGTTG TGCAAGGAAC GATTCCATAC 
CTTGGCACTT TTCTGACTGA CCTCGTCATG TTGGATACTG CTATGAAAGA CCATCTGGAG 
GTCGGACTGA TTAACTTTGA GAAGAGAAGA AAGGAATTTG AAGTGATCGC TCAGATCAAG 
TTGCTGCAGC TGACCTGCAA TTATTACAAC TTCAGCAGAA ACCAGCGCTT TATTGACTGG 
TTTAAGAGAG TGGAGAAACT CACAGAGACC GAGAGCTACT CTATGTCGTG TGATATCGAG 
CCGCCGTCTG AGTCGTTGTG CAAAAAGAAT GGAGGCATCA TGAAGCGCAT GAGCGAGGAG 
TCGGGCTACA GCAGCGCTGC GTCACACTCC AAGTCCTTTG AGCAGCCGCG ATTAAGCCAG 
TTCAAAGACT GCTTTAAAGA TGGAGCTGAT TCCCTCAGCC TCACCTCTGC AGGATCCAGT 
GGCTCAGATG CAGACGAGAG TCTCAGCCTG CTCAACTCTC CTGAATCAGG CAGTCAGAGA 
ACATCCACAC CAACTGTGAA ACACTCATCA ACCTCAGAAA CAGGAGAGCT CCCGTCTGAT 
TCCTCAACCA AGCTCTGGGA ATCACCCACC CCATCATCTC TGGAGGCGTC TGGATCAGGC 
TTGTGTTTAG AGTCAGGCTG CAGCAGCTCC ACATCTTCCT CCTCTTCCTC CTCGTCCTCA 
GTCTCAGCCT TCACAGGACG GACCTTCAAC TTTCACAAGC GCTCAGTCTC TGGCATCTCA 
GACTACTCGT CTCTCAGTCT GCCGCTCTAC AACCAGCAGG CAAACGACTG CTGCATCATC 
AGAGTCAGCC TGGACTCAGA AAACGGCAAC ATGTACAAAA GTATCCTGGT CACTAGTCAG 
GACAAAACCC CTGGGGTCAT TAGGAAAGCC ATGGCCAAAC ACAACCTGGA CAATGACAAA 
TCTGAAGACT ATGAATTGTT ACAGAGGGTC TCAAAGCATA AAGAACTCAG AATCCCAGAT 
AATGGAAACG TTTTCTACGC CATGAACTCA ACTGCCAACT ACGACTTCTT GTTGAGAAAG 
CGTGGCGTCC CAAAAACCTG CCGCTCGAAA AGCTCCCCGA GCCTGACTCT ACCACGCATG 
AAGCAGAAAG GCTTCAAAAT CCCCAAGAGC TTCTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

si:ch211-251j10.3 ( XM_009304057.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009302332.1 Danio rerio si:ch211-251j10.3 (si:ch211-251j10.3), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa47376
Clone ID Related Accession (Same CDS sequence)	XM_009304057.2 , XM_009304057.3
Accession Version	XM_009304057.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2499bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	ral guanine nucleotide dissociation stimulator isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009304057.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAGCAG ACAAGGGGGA GCAGCATTCC CCTAATATGA AGCAGGCGCC TGGACAGCAG 
CCTACACTCT CTCCATCGGG TAAGATGGAG CCCAAATCTC TGTTCAGCCT GCATAAAGCT 
CTGGCTCAAC CTGTGAAGAT GTGCATGTTT GATTTCCCCG TCTCCATCCT GGACGATCTG 
AGCTCAGTTC AGGAGATTGG AGAGGAGGTG GAGGACGGTG CCATCTATAC CATTACACTC 
CGAAAGGTGC AGCTCCACCA GACGGCCAGT AAGGGCCAGC GATGGCTGGG CGTGGAGACA 
GACTCTGCCC TCAGTCTGTA CGAGACGTGC AAGGGTCGAA CCATTAAGGC TGGCACACTG 
GAGAAACTAG TGGAGTACAT GGTCTCCGCC TTCAAAGGGA AAGACTACAC TTACGTCACT 
ATTTTCCTCT GCACCTACAG AGCATTTGCC ACCACCAAAC AAGTGCTAGA CCTGCTGCTT 
AACAGATATG CCAAACTGCC AGCCTCAGGC AAGACTAAAG TCTCAGCAGA AGACCACACA 
GAGCTTAAAA ACACTGTGTC ATCTATCCTG GGTGCCTGGT TAGATCAATA CTCGGAGGAT 
TTCTGGAGTC CTCCAGATCA CGAATGCCTG CAGAGTCTCA TATCCTACTT ACAACTCAAC 
TTCTCTGGAT CGGACCTGGA GAGACGAGCT TTAAACCTGC TGGAGCACTT CCAGCATCGC 
CAACAGTGCG AGGTGGAGAT TGAAGGTGAC CTGTGCTCCT GCCCCTTTGC CACACCAGAG 
GAGAGCAGTT TGGAGGATGA ATTCTCCCCC TCACATTTCA TGTCCTTCAG TCCTTCTATA 
GTTGCTGAGC AGTTAACAGT CATGGATGCG GAGCTGTTTA AGAGGGTTGT TCCATATCAT 
TGTTTAGGGG GCATATGGTC CCAGAGAGAT AAAAAGGGCA AAGAGCACCT GGCACCAACC 
ATCCGTGCTA CTGTTTCTCA GTTCAACAGT GTGACCAACT GTGTGATCGC CACCTGCCTG 
GACAACAGGT CTCTCAGACC TTTCCAAAGA GCCAAGCGCA TTGAACATTG GATAGAAGTT 
GCCAGGAAAT GCCGTATCCT GAAGAGCTTC TCCTCTCTGA AAGCCATCCT GTCTGCCTTG 
CAGAGTAATG CCATCCACAG GCTGAGAAGG GCCTGGGATG ATGTTTCACG GGAAAGCTAT 
CGAACTTTCC AAGAGCTGTC TGAAATCTTC TCAGACGACA ATAATTACTC ACTCAGCAGA 
GAGCTTCTCA TTAAGGAGGG CACCTCTAAA TCTGCCATAG TCGAGATAAA CCACAAAGGA 
GCCCAGAGGA GACACCAGCA GCAAAGAGAC ATGGGTGTTG TGCAAGGAAC GATTCCATAC 
CTTGGCACTT TTCTGACTGA CCTCGTCATG TTGGATACTG CTATGAAAGA CCATCTGGAG 
GTCGGACTGA TTAACTTTGA GAAGAGAAGA AAGGAATTTG AAGTGATCGC TCAGATCAAG 
TTGCTGCAGC TGACCTGCAA TTATTACAAC TTCAGCAGAA ACCAGCGCTT TATTGACTGG 
TTTAAGAGAG TGGAGAAACT CACAGAGACC GAGAGCTACT CTATGTCGTG TGATATCGAG 
CCGCCGTCTG AGTCGTTGTG CAAAAAGAAT GGAGGCATCA TGAAGCGCAT GAGCGAGGAG 
TCGGGCTACA GCAGCGCTGC GTCACACTCC AAGTCCTTTG AGCAGCCGCG ATTAAGCCAG 
TTCAAAGACT GCTTTAAAGA TGGAGCTGAT TCCCTCAGCC TCACCTCTGC AGGATCCAGT 
GGCTCAGATG CAGACGAGAG TCTCAGCCTG CTCAACTCTC CTGAATCAGG CAGTCAGAGA 
ACATCCACAC CAACTGTGAA ACACTCATCA ACCTCAGAAA CAGGAGAGCT CCCGTCTGAT 
TCCTCAACCA AGCTCTGGGA ATCACCCACC CCATCATCTC TGGAGGCGTC TGGATCAGGC 
TTGTGTTTAG AGTCAGGCTG CAGCAGCTCC ACATCTTCCT CCTCTTCCTC CTCGTCCTCA 
GTCTCAGCCT TCACAGGACG GACCTTCAAC TTTCACAAGC GCTCAGTCTC TGGCATCTCA 
GACTACTCGT CTCTCAGTCT GCCGCTCTAC AACCAGCAGG CAAACGACTG CTGCATCATC 
AGAGTCAGCC TGGACTCAGA AAACGGCAAC ATGTACAAAA GTATCCTGGT CACTAGTCAG 
GACAAAACCC CTGGGGTCAT TAGGAAAGCC ATGGCCAAAC ACAACCTGGA CAATGACAAA 
TCTGAAGACT ATGAATTGTT ACAGAGGGTC TCAAAGCATA AAGAACTCAG AATCCCAGAT 
AATGGAAACG TTTTCTACGC CATGAACTCA ACTGCCAACT ACGACTTCTT GTTGAGAAAG 
CGTGGCGTCC CAAAAACCTG CCGCTCGAAA AGCTCCCCGA GCCTGACTCT ACCACGCATG 
AAGCAGAAAG GCTTCAAAAT CCCCAAGAGC TTCTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009302332.1
CDS	143..2641
Translation	MRADKGEQHSPNMKQAPGQQPTLSPSGKMEPKSLFSLHKALAQPVKMCMFDFPVSILDDLSSVQEIGEEVEDGAIYTITLRKVQLHQTASKGQRWLGVETDSALSLYETCKGRTIKAGTLEKLVEYMVSAFKGKDYTYVTIFLCTYRAFATTKQVLDLLLNRYAKLPASGKTKVSAEDHTELKNTVSSILGAWLDQYSEDFWSPPDHECLQSLISYLQLNFSGSDLERRALNLLEHFQHRQQCEVEIEGDLCSCPFATPEESSLEDEFSPSHFMSFSPSIVAEQLTVMDAELFKRVVPYHCLGGIWSQRDKKGKEHLAPTIRATVSQFNSVTNCVIATCLDNRSLRPFQRAKRIEHWIEVARKCRILKSFSSLKAILSALQSNAIHRLRRAWDDVSRESYRTFQELSEIFSDDNNYSLSRELLIKEGTSKSAIVEINHKGAQRRHQQQRDMGVVQGTIPYLGTFLTDLVMLDTAMKDHLEVGLINFEKRRKEFEVIAQIKLLQLTCNYYNFSRNQRFIDWFKRVEKLTETESYSMSCDIEPPSESLCKKNGGIMKRMSEESGYSSAASHSKSFEQPRLSQFKDCFKDGADSLSLTSAGSSGSDADESLSLLNSPESGSQRTSTPTVKHSSTSETGELPSDSSTKLWESPTPSSLEASGSGLCLESGCSSSTSSSSSSSSSVSAFTGRTFNFHKRSVSGISDYSSLSLPLYNQQANDCCIIRVSLDSENGNMYKSILVTSQDKTPGVIRKAMAKHNLDNDKSEDYELLQRVSKHKELRIPDNGNVFYAMNSTANYDFLLRKRGVPKTCRSKSSPSLTLPRMKQKGFKIPKSFF

Target ORF information:

RefSeq Version	XM_009304057.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio si:ch211-251j10.3 (si:ch211-251j10.3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009304057.3

ORF Insert Sequence:

ATGAGAGCAG ACAAGGGGGA GCAGCATTCC CCTAATATGA AGCAGGCGCC TGGACAGCAG 
CCTACACTCT CTCCATCGGG TAAGATGGAG CCCAAATCTC TGTTCAGCCT GCATAAAGCT 
CTGGCTCAAC CTGTGAAGAT GTGCATGTTT GATTTCCCCG TCTCCATCCT GGACGATCTG 
AGCTCAGTTC AGGAGATTGG AGAGGAGGTG GAGGACGGTG CCATCTATAC CATTACACTC 
CGAAAGGTGC AGCTCCACCA GACGGCCAGT AAGGGCCAGC GATGGCTGGG CGTGGAGACA 
GACTCTGCCC TCAGTCTGTA CGAGACGTGC AAGGGTCGAA CCATTAAGGC TGGCACACTG 
GAGAAACTAG TGGAGTACAT GGTCTCCGCC TTCAAAGGGA AAGACTACAC TTACGTCACT 
ATTTTCCTCT GCACCTACAG AGCATTTGCC ACCACCAAAC AAGTGCTAGA CCTGCTGCTT 
AACAGATATG CCAAACTGCC AGCCTCAGGC AAGACTAAAG TCTCAGCAGA AGACCACACA 
GAGCTTAAAA ACACTGTGTC ATCTATCCTG GGTGCCTGGT TAGATCAATA CTCGGAGGAT 
TTCTGGAGTC CTCCAGATCA CGAATGCCTG CAGAGTCTCA TATCCTACTT ACAACTCAAC 
TTCTCTGGAT CGGACCTGGA GAGACGAGCT TTAAACCTGC TGGAGCACTT CCAGCATCGC 
CAACAGTGCG AGGTGGAGAT TGAAGGTGAC CTGTGCTCCT GCCCCTTTGC CACACCAGAG 
GAGAGCAGTT TGGAGGATGA ATTCTCCCCC TCACATTTCA TGTCCTTCAG TCCTTCTATA 
GTTGCTGAGC AGTTAACAGT CATGGATGCG GAGCTGTTTA AGAGGGTTGT TCCATATCAT 
TGTTTAGGGG GCATATGGTC CCAGAGAGAT AAAAAGGGCA AAGAGCACCT GGCACCAACC 
ATCCGTGCTA CTGTTTCTCA GTTCAACAGT GTGACCAACT GTGTGATCGC CACCTGCCTG 
GACAACAGGT CTCTCAGACC TTTCCAAAGA GCCAAGCGCA TTGAACATTG GATAGAAGTT 
GCCAGGAAAT GCCGTATCCT GAAGAGCTTC TCCTCTCTGA AAGCCATCCT GTCTGCCTTG 
CAGAGTAATG CCATCCACAG GCTGAGAAGG GCCTGGGATG ATGTTTCACG GGAAAGCTAT 
CGAACTTTCC AAGAGCTGTC TGAAATCTTC TCAGACGACA ATAATTACTC ACTCAGCAGA 
GAGCTTCTCA TTAAGGAGGG CACCTCTAAA TCTGCCATAG TCGAGATAAA CCACAAAGGA 
GCCCAGAGGA GACACCAGCA GCAAAGAGAC ATGGGTGTTG TGCAAGGAAC GATTCCATAC 
CTTGGCACTT TTCTGACTGA CCTCGTCATG TTGGATACTG CTATGAAAGA CCATCTGGAG 
GTCGGACTGA TTAACTTTGA GAAGAGAAGA AAGGAATTTG AAGTGATCGC TCAGATCAAG 
TTGCTGCAGC TGACCTGCAA TTATTACAAC TTCAGCAGAA ACCAGCGCTT TATTGACTGG 
TTTAAGAGAG TGGAGAAACT CACAGAGACC GAGAGCTACT CTATGTCGTG TGATATCGAG 
CCGCCGTCTG AGTCGTTGTG CAAAAAGAAT GGAGGCATCA TGAAGCGCAT GAGCGAGGAG 
TCGGGCTACA GCAGCGCTGC GTCACACTCC AAGTCCTTTG AGCAGCCGCG ATTAAGCCAG 
TTCAAAGACT GCTTTAAAGA TGGAGCTGAT TCCCTCAGCC TCACCTCTGC AGGATCCAGT 
GGCTCAGATG CAGACGAGAG TCTCAGCCTG CTCAACTCTC CTGAATCAGG CAGTCAGAGA 
ACATCCACAC CAACTGTGAA ACACTCATCA ACCTCAGAAA CAGGAGAGCT CCCGTCTGAT 
TCCTCAACCA AGCTCTGGGA ATCACCCACC CCATCATCTC TGGAGGCGTC TGGATCAGGC 
TTGTGTTTAG AGTCAGGCTG CAGCAGCTCC ACATCTTCCT CCTCTTCCTC CTCGTCCTCA 
GTCTCAGCCT TCACAGGACG GACCTTCAAC TTTCACAAGC GCTCAGTCTC TGGCATCTCA 
GACTACTCGT CTCTCAGTCT GCCGCTCTAC AACCAGCAGG CAAACGACTG CTGCATCATC 
AGAGTCAGCC TGGACTCAGA AAACGGCAAC ATGTACAAAA GTATCCTGGT CACTAGTCAG 
GACAAAACCC CTGGGGTCAT TAGGAAAGCC ATGGCCAAAC ACAACCTGGA CAATGACAAA 
TCTGAAGACT ATGAATTGTT ACAGAGGGTC TCAAAGCATA AAGAACTCAG AATCCCAGAT 
AATGGAAACG TTTTCTACGC CATGAACTCA ACTGCCAACT ACGACTTCTT GTTGAGAAAG 
CGTGGCGTCC CAAAAACCTG CCGCTCGAAA AGCTCCCCGA GCCTGACTCT ACCACGCATG 
AAGCAGAAAG GCTTCAAAAT CCCCAAGAGC TTCTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

si:ch211-251j10.3 ( XM_009304058.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009302333.1 Danio rerio si:ch211-251j10.3 (si:ch211-251j10.3), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa47377
Clone ID Related Accession (Same CDS sequence)	XM_009304058.3 , XM_009304058.2
Accession Version	XM_009304058.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2439bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	ral guanine nucleotide dissociation stimulator isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009304058.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTTGGA AAGACAGGAT TGATCAACTC ATTCAACTGA TACGCTGGTG TGGCTTGGGT 
TGTATGTACG TGAATGGAGA TCTGTACTGT GAGTTGGGAG ATTTAGAGGA ACGGAAACAG 
AGCTCAGTTC AGGAGATTGG AGAGGAGGTG GAGGACGGTG CCATCTATAC CATTACACTC 
CGAAAGGTGC AGCTCCACCA GACGGCCAGT AAGGGCCAGC GATGGCTGGG CGTGGAGACA 
GACTCTGCCC TCAGTCTGTA CGAGACGTGC AAGGGTCGAA CCATTAAGGC TGGCACACTG 
GAGAAACTAG TGGAGTACAT GGTCTCCGCC TTCAAAGGGA AAGACTACAC TTACGTCACT 
ATTTTCCTCT GCACCTACAG AGCATTTGCC ACCACCAAAC AAGTGCTAGA CCTGCTGCTT 
AACAGATATG CCAAACTGCC AGCCTCAGGC AAGACTAAAG TCTCAGCAGA AGACCACACA 
GAGCTTAAAA ACACTGTGTC ATCTATCCTG GGTGCCTGGT TAGATCAATA CTCGGAGGAT 
TTCTGGAGTC CTCCAGATCA CGAATGCCTG CAGAGTCTCA TATCCTACTT ACAACTCAAC 
TTCTCTGGAT CGGACCTGGA GAGACGAGCT TTAAACCTGC TGGAGCACTT CCAGCATCGC 
CAACAGTGCG AGGTGGAGAT TGAAGGTGAC CTGTGCTCCT GCCCCTTTGC CACACCAGAG 
GAGAGCAGTT TGGAGGATGA ATTCTCCCCC TCACATTTCA TGTCCTTCAG TCCTTCTATA 
GTTGCTGAGC AGTTAACAGT CATGGATGCG GAGCTGTTTA AGAGGGTTGT TCCATATCAT 
TGTTTAGGGG GCATATGGTC CCAGAGAGAT AAAAAGGGCA AAGAGCACCT GGCACCAACC 
ATCCGTGCTA CTGTTTCTCA GTTCAACAGT GTGACCAACT GTGTGATCGC CACCTGCCTG 
GACAACAGGT CTCTCAGACC TTTCCAAAGA GCCAAGCGCA TTGAACATTG GATAGAAGTT 
GCCAGGAAAT GCCGTATCCT GAAGAGCTTC TCCTCTCTGA AAGCCATCCT GTCTGCCTTG 
CAGAGTAATG CCATCCACAG GCTGAGAAGG GCCTGGGATG ATGTTTCACG GGAAAGCTAT 
CGAACTTTCC AAGAGCTGTC TGAAATCTTC TCAGACGACA ATAATTACTC ACTCAGCAGA 
GAGCTTCTCA TTAAGGAGGG CACCTCTAAA TCTGCCATAG TCGAGATAAA CCACAAAGGA 
GCCCAGAGGA GACACCAGCA GCAAAGAGAC ATGGGTGTTG TGCAAGGAAC GATTCCATAC 
CTTGGCACTT TTCTGACTGA CCTCGTCATG TTGGATACTG CTATGAAAGA CCATCTGGAG 
GTCGGACTGA TTAACTTTGA GAAGAGAAGA AAGGAATTTG AAGTGATCGC TCAGATCAAG 
TTGCTGCAGC TGACCTGCAA TTATTACAAC TTCAGCAGAA ACCAGCGCTT TATTGACTGG 
TTTAAGAGAG TGGAGAAACT CACAGAGACC GAGAGCTACT CTATGTCGTG TGATATCGAG 
CCGCCGTCTG AGTCGTTGTG CAAAAAGAAT GGAGGCATCA TGAAGCGCAT GAGCGAGGAG 
TCGGGCTACA GCAGCGCTGC GTCACACTCC AAGTCCTTTG AGCAGCCGCG ATTAAGCCAG 
TTCAAAGACT GCTTTAAAGA TGGAGCTGAT TCCCTCAGCC TCACCTCTGC AGGATCCAGT 
GGCTCAGATG CAGACGAGAG TCTCAGCCTG CTCAACTCTC CTGAATCAGG CAGTCAGAGA 
ACATCCACAC CAACTGTGAA ACACTCATCA ACCTCAGAAA CAGGAGAGCT CCCGTCTGAT 
TCCTCAACCA AGCTCTGGGA ATCACCCACC CCATCATCTC TGGAGGCGTC TGGATCAGGC 
TTGTGTTTAG AGTCAGGCTG CAGCAGCTCC ACATCTTCCT CCTCTTCCTC CTCGTCCTCA 
GTCTCAGCCT TCACAGGACG GACCTTCAAC TTTCACAAGC GCTCAGTCTC TGGCATCTCA 
GACTACTCGT CTCTCAGTCT GCCGCTCTAC AACCAGCAGG CAAACGACTG CTGCATCATC 
AGAGTCAGCC TGGACTCAGA AAACGGCAAC ATGTACAAAA GTATCCTGGT CACTAGTCAG 
GACAAAACCC CTGGGGTCAT TAGGAAAGCC ATGGCCAAAC ACAACCTGGA CAATGACAAA 
TCTGAAGACT ATGAATTGTT ACAGAGGGTC TCAAAGCATA AAGAACTCAG AATCCCAGAT 
AATGGAAACG TTTTCTACGC CATGAACTCA ACTGCCAACT ACGACTTCTT GTTGAGAAAG 
CGTGGCGTCC CAAAAACCTG CCGCTCGAAA AGCTCCCCGA GCCTGACTCT ACCACGCATG 
AAGCAGAAAG GCTTCAAAAT CCCCAAGAGC TTCTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009302333.1
CDS	900..3338
Translation	MCWKDRIDQLIQLIRWCGLGCMYVNGDLYCELGDLEERKQSSVQEIGEEVEDGAIYTITLRKVQLHQTASKGQRWLGVETDSALSLYETCKGRTIKAGTLEKLVEYMVSAFKGKDYTYVTIFLCTYRAFATTKQVLDLLLNRYAKLPASGKTKVSAEDHTELKNTVSSILGAWLDQYSEDFWSPPDHECLQSLISYLQLNFSGSDLERRALNLLEHFQHRQQCEVEIEGDLCSCPFATPEESSLEDEFSPSHFMSFSPSIVAEQLTVMDAELFKRVVPYHCLGGIWSQRDKKGKEHLAPTIRATVSQFNSVTNCVIATCLDNRSLRPFQRAKRIEHWIEVARKCRILKSFSSLKAILSALQSNAIHRLRRAWDDVSRESYRTFQELSEIFSDDNNYSLSRELLIKEGTSKSAIVEINHKGAQRRHQQQRDMGVVQGTIPYLGTFLTDLVMLDTAMKDHLEVGLINFEKRRKEFEVIAQIKLLQLTCNYYNFSRNQRFIDWFKRVEKLTETESYSMSCDIEPPSESLCKKNGGIMKRMSEESGYSSAASHSKSFEQPRLSQFKDCFKDGADSLSLTSAGSSGSDADESLSLLNSPESGSQRTSTPTVKHSSTSETGELPSDSSTKLWESPTPSSLEASGSGLCLESGCSSSTSSSSSSSSSVSAFTGRTFNFHKRSVSGISDYSSLSLPLYNQQANDCCIIRVSLDSENGNMYKSILVTSQDKTPGVIRKAMAKHNLDNDKSEDYELLQRVSKHKELRIPDNGNVFYAMNSTANYDFLLRKRGVPKTCRSKSSPSLTLPRMKQKGFKIPKSFF

Target ORF information:

RefSeq Version	XM_009304058.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio si:ch211-251j10.3 (si:ch211-251j10.3), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009304058.3

ORF Insert Sequence:

ATGTGTTGGA AAGACAGGAT TGATCAACTC ATTCAACTGA TACGCTGGTG TGGCTTGGGT 
TGTATGTACG TGAATGGAGA TCTGTACTGT GAGTTGGGAG ATTTAGAGGA ACGGAAACAG 
AGCTCAGTTC AGGAGATTGG AGAGGAGGTG GAGGACGGTG CCATCTATAC CATTACACTC 
CGAAAGGTGC AGCTCCACCA GACGGCCAGT AAGGGCCAGC GATGGCTGGG CGTGGAGACA 
GACTCTGCCC TCAGTCTGTA CGAGACGTGC AAGGGTCGAA CCATTAAGGC TGGCACACTG 
GAGAAACTAG TGGAGTACAT GGTCTCCGCC TTCAAAGGGA AAGACTACAC TTACGTCACT 
ATTTTCCTCT GCACCTACAG AGCATTTGCC ACCACCAAAC AAGTGCTAGA CCTGCTGCTT 
AACAGATATG CCAAACTGCC AGCCTCAGGC AAGACTAAAG TCTCAGCAGA AGACCACACA 
GAGCTTAAAA ACACTGTGTC ATCTATCCTG GGTGCCTGGT TAGATCAATA CTCGGAGGAT 
TTCTGGAGTC CTCCAGATCA CGAATGCCTG CAGAGTCTCA TATCCTACTT ACAACTCAAC 
TTCTCTGGAT CGGACCTGGA GAGACGAGCT TTAAACCTGC TGGAGCACTT CCAGCATCGC 
CAACAGTGCG AGGTGGAGAT TGAAGGTGAC CTGTGCTCCT GCCCCTTTGC CACACCAGAG 
GAGAGCAGTT TGGAGGATGA ATTCTCCCCC TCACATTTCA TGTCCTTCAG TCCTTCTATA 
GTTGCTGAGC AGTTAACAGT CATGGATGCG GAGCTGTTTA AGAGGGTTGT TCCATATCAT 
TGTTTAGGGG GCATATGGTC CCAGAGAGAT AAAAAGGGCA AAGAGCACCT GGCACCAACC 
ATCCGTGCTA CTGTTTCTCA GTTCAACAGT GTGACCAACT GTGTGATCGC CACCTGCCTG 
GACAACAGGT CTCTCAGACC TTTCCAAAGA GCCAAGCGCA TTGAACATTG GATAGAAGTT 
GCCAGGAAAT GCCGTATCCT GAAGAGCTTC TCCTCTCTGA AAGCCATCCT GTCTGCCTTG 
CAGAGTAATG CCATCCACAG GCTGAGAAGG GCCTGGGATG ATGTTTCACG GGAAAGCTAT 
CGAACTTTCC AAGAGCTGTC TGAAATCTTC TCAGACGACA ATAATTACTC ACTCAGCAGA 
GAGCTTCTCA TTAAGGAGGG CACCTCTAAA TCTGCCATAG TCGAGATAAA CCACAAAGGA 
GCCCAGAGGA GACACCAGCA GCAAAGAGAC ATGGGTGTTG TGCAAGGAAC GATTCCATAC 
CTTGGCACTT TTCTGACTGA CCTCGTCATG TTGGATACTG CTATGAAAGA CCATCTGGAG 
GTCGGACTGA TTAACTTTGA GAAGAGAAGA AAGGAATTTG AAGTGATCGC TCAGATCAAG 
TTGCTGCAGC TGACCTGCAA TTATTACAAC TTCAGCAGAA ACCAGCGCTT TATTGACTGG 
TTTAAGAGAG TGGAGAAACT CACAGAGACC GAGAGCTACT CTATGTCGTG TGATATCGAG 
CCGCCGTCTG AGTCGTTGTG CAAAAAGAAT GGAGGCATCA TGAAGCGCAT GAGCGAGGAG 
TCGGGCTACA GCAGCGCTGC GTCACACTCC AAGTCCTTTG AGCAGCCGCG ATTAAGCCAG 
TTCAAAGACT GCTTTAAAGA TGGAGCTGAT TCCCTCAGCC TCACCTCTGC AGGATCCAGT 
GGCTCAGATG CAGACGAGAG TCTCAGCCTG CTCAACTCTC CTGAATCAGG CAGTCAGAGA 
ACATCCACAC CAACTGTGAA ACACTCATCA ACCTCAGAAA CAGGAGAGCT CCCGTCTGAT 
TCCTCAACCA AGCTCTGGGA ATCACCCACC CCATCATCTC TGGAGGCGTC TGGATCAGGC 
TTGTGTTTAG AGTCAGGCTG CAGCAGCTCC ACATCTTCCT CCTCTTCCTC CTCGTCCTCA 
GTCTCAGCCT TCACAGGACG GACCTTCAAC TTTCACAAGC GCTCAGTCTC TGGCATCTCA 
GACTACTCGT CTCTCAGTCT GCCGCTCTAC AACCAGCAGG CAAACGACTG CTGCATCATC 
AGAGTCAGCC TGGACTCAGA AAACGGCAAC ATGTACAAAA GTATCCTGGT CACTAGTCAG 
GACAAAACCC CTGGGGTCAT TAGGAAAGCC ATGGCCAAAC ACAACCTGGA CAATGACAAA 
TCTGAAGACT ATGAATTGTT ACAGAGGGTC TCAAAGCATA AAGAACTCAG AATCCCAGAT 
AATGGAAACG TTTTCTACGC CATGAACTCA ACTGCCAACT ACGACTTCTT GTTGAGAAAG 
CGTGGCGTCC CAAAAACCTG CCGCTCGAAA AGCTCCCCGA GCCTGACTCT ACCACGCATG 
AAGCAGAAAG GCTTCAAAAT CCCCAAGAGC TTCTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

si:ch211-251j10.3 ( XM_009304058.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009302333.1 Danio rerio si:ch211-251j10.3 (si:ch211-251j10.3), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa47377
Clone ID Related Accession (Same CDS sequence)	XM_009304058.3 , XM_009304058.2
Accession Version	XM_009304058.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2439bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	ral guanine nucleotide dissociation stimulator isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009304058.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTTGGA AAGACAGGAT TGATCAACTC ATTCAACTGA TACGCTGGTG TGGCTTGGGT 
TGTATGTACG TGAATGGAGA TCTGTACTGT GAGTTGGGAG ATTTAGAGGA ACGGAAACAG 
AGCTCAGTTC AGGAGATTGG AGAGGAGGTG GAGGACGGTG CCATCTATAC CATTACACTC 
CGAAAGGTGC AGCTCCACCA GACGGCCAGT AAGGGCCAGC GATGGCTGGG CGTGGAGACA 
GACTCTGCCC TCAGTCTGTA CGAGACGTGC AAGGGTCGAA CCATTAAGGC TGGCACACTG 
GAGAAACTAG TGGAGTACAT GGTCTCCGCC TTCAAAGGGA AAGACTACAC TTACGTCACT 
ATTTTCCTCT GCACCTACAG AGCATTTGCC ACCACCAAAC AAGTGCTAGA CCTGCTGCTT 
AACAGATATG CCAAACTGCC AGCCTCAGGC AAGACTAAAG TCTCAGCAGA AGACCACACA 
GAGCTTAAAA ACACTGTGTC ATCTATCCTG GGTGCCTGGT TAGATCAATA CTCGGAGGAT 
TTCTGGAGTC CTCCAGATCA CGAATGCCTG CAGAGTCTCA TATCCTACTT ACAACTCAAC 
TTCTCTGGAT CGGACCTGGA GAGACGAGCT TTAAACCTGC TGGAGCACTT CCAGCATCGC 
CAACAGTGCG AGGTGGAGAT TGAAGGTGAC CTGTGCTCCT GCCCCTTTGC CACACCAGAG 
GAGAGCAGTT TGGAGGATGA ATTCTCCCCC TCACATTTCA TGTCCTTCAG TCCTTCTATA 
GTTGCTGAGC AGTTAACAGT CATGGATGCG GAGCTGTTTA AGAGGGTTGT TCCATATCAT 
TGTTTAGGGG GCATATGGTC CCAGAGAGAT AAAAAGGGCA AAGAGCACCT GGCACCAACC 
ATCCGTGCTA CTGTTTCTCA GTTCAACAGT GTGACCAACT GTGTGATCGC CACCTGCCTG 
GACAACAGGT CTCTCAGACC TTTCCAAAGA GCCAAGCGCA TTGAACATTG GATAGAAGTT 
GCCAGGAAAT GCCGTATCCT GAAGAGCTTC TCCTCTCTGA AAGCCATCCT GTCTGCCTTG 
CAGAGTAATG CCATCCACAG GCTGAGAAGG GCCTGGGATG ATGTTTCACG GGAAAGCTAT 
CGAACTTTCC AAGAGCTGTC TGAAATCTTC TCAGACGACA ATAATTACTC ACTCAGCAGA 
GAGCTTCTCA TTAAGGAGGG CACCTCTAAA TCTGCCATAG TCGAGATAAA CCACAAAGGA 
GCCCAGAGGA GACACCAGCA GCAAAGAGAC ATGGGTGTTG TGCAAGGAAC GATTCCATAC 
CTTGGCACTT TTCTGACTGA CCTCGTCATG TTGGATACTG CTATGAAAGA CCATCTGGAG 
GTCGGACTGA TTAACTTTGA GAAGAGAAGA AAGGAATTTG AAGTGATCGC TCAGATCAAG 
TTGCTGCAGC TGACCTGCAA TTATTACAAC TTCAGCAGAA ACCAGCGCTT TATTGACTGG 
TTTAAGAGAG TGGAGAAACT CACAGAGACC GAGAGCTACT CTATGTCGTG TGATATCGAG 
CCGCCGTCTG AGTCGTTGTG CAAAAAGAAT GGAGGCATCA TGAAGCGCAT GAGCGAGGAG 
TCGGGCTACA GCAGCGCTGC GTCACACTCC AAGTCCTTTG AGCAGCCGCG ATTAAGCCAG 
TTCAAAGACT GCTTTAAAGA TGGAGCTGAT TCCCTCAGCC TCACCTCTGC AGGATCCAGT 
GGCTCAGATG CAGACGAGAG TCTCAGCCTG CTCAACTCTC CTGAATCAGG CAGTCAGAGA 
ACATCCACAC CAACTGTGAA ACACTCATCA ACCTCAGAAA CAGGAGAGCT CCCGTCTGAT 
TCCTCAACCA AGCTCTGGGA ATCACCCACC CCATCATCTC TGGAGGCGTC TGGATCAGGC 
TTGTGTTTAG AGTCAGGCTG CAGCAGCTCC ACATCTTCCT CCTCTTCCTC CTCGTCCTCA 
GTCTCAGCCT TCACAGGACG GACCTTCAAC TTTCACAAGC GCTCAGTCTC TGGCATCTCA 
GACTACTCGT CTCTCAGTCT GCCGCTCTAC AACCAGCAGG CAAACGACTG CTGCATCATC 
AGAGTCAGCC TGGACTCAGA AAACGGCAAC ATGTACAAAA GTATCCTGGT CACTAGTCAG 
GACAAAACCC CTGGGGTCAT TAGGAAAGCC ATGGCCAAAC ACAACCTGGA CAATGACAAA 
TCTGAAGACT ATGAATTGTT ACAGAGGGTC TCAAAGCATA AAGAACTCAG AATCCCAGAT 
AATGGAAACG TTTTCTACGC CATGAACTCA ACTGCCAACT ACGACTTCTT GTTGAGAAAG 
CGTGGCGTCC CAAAAACCTG CCGCTCGAAA AGCTCCCCGA GCCTGACTCT ACCACGCATG 
AAGCAGAAAG GCTTCAAAAT CCCCAAGAGC TTCTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009302333.1
CDS	588..3026
Translation	MCWKDRIDQLIQLIRWCGLGCMYVNGDLYCELGDLEERKQSSVQEIGEEVEDGAIYTITLRKVQLHQTASKGQRWLGVETDSALSLYETCKGRTIKAGTLEKLVEYMVSAFKGKDYTYVTIFLCTYRAFATTKQVLDLLLNRYAKLPASGKTKVSAEDHTELKNTVSSILGAWLDQYSEDFWSPPDHECLQSLISYLQLNFSGSDLERRALNLLEHFQHRQQCEVEIEGDLCSCPFATPEESSLEDEFSPSHFMSFSPSIVAEQLTVMDAELFKRVVPYHCLGGIWSQRDKKGKEHLAPTIRATVSQFNSVTNCVIATCLDNRSLRPFQRAKRIEHWIEVARKCRILKSFSSLKAILSALQSNAIHRLRRAWDDVSRESYRTFQELSEIFSDDNNYSLSRELLIKEGTSKSAIVEINHKGAQRRHQQQRDMGVVQGTIPYLGTFLTDLVMLDTAMKDHLEVGLINFEKRRKEFEVIAQIKLLQLTCNYYNFSRNQRFIDWFKRVEKLTETESYSMSCDIEPPSESLCKKNGGIMKRMSEESGYSSAASHSKSFEQPRLSQFKDCFKDGADSLSLTSAGSSGSDADESLSLLNSPESGSQRTSTPTVKHSSTSETGELPSDSSTKLWESPTPSSLEASGSGLCLESGCSSSTSSSSSSSSSVSAFTGRTFNFHKRSVSGISDYSSLSLPLYNQQANDCCIIRVSLDSENGNMYKSILVTSQDKTPGVIRKAMAKHNLDNDKSEDYELLQRVSKHKELRIPDNGNVFYAMNSTANYDFLLRKRGVPKTCRSKSSPSLTLPRMKQKGFKIPKSFF

Target ORF information:

RefSeq Version	XM_009304058.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio si:ch211-251j10.3 (si:ch211-251j10.3), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009304058.2

ORF Insert Sequence:

ATGTGTTGGA AAGACAGGAT TGATCAACTC ATTCAACTGA TACGCTGGTG TGGCTTGGGT 
TGTATGTACG TGAATGGAGA TCTGTACTGT GAGTTGGGAG ATTTAGAGGA ACGGAAACAG 
AGCTCAGTTC AGGAGATTGG AGAGGAGGTG GAGGACGGTG CCATCTATAC CATTACACTC 
CGAAAGGTGC AGCTCCACCA GACGGCCAGT AAGGGCCAGC GATGGCTGGG CGTGGAGACA 
GACTCTGCCC TCAGTCTGTA CGAGACGTGC AAGGGTCGAA CCATTAAGGC TGGCACACTG 
GAGAAACTAG TGGAGTACAT GGTCTCCGCC TTCAAAGGGA AAGACTACAC TTACGTCACT 
ATTTTCCTCT GCACCTACAG AGCATTTGCC ACCACCAAAC AAGTGCTAGA CCTGCTGCTT 
AACAGATATG CCAAACTGCC AGCCTCAGGC AAGACTAAAG TCTCAGCAGA AGACCACACA 
GAGCTTAAAA ACACTGTGTC ATCTATCCTG GGTGCCTGGT TAGATCAATA CTCGGAGGAT 
TTCTGGAGTC CTCCAGATCA CGAATGCCTG CAGAGTCTCA TATCCTACTT ACAACTCAAC 
TTCTCTGGAT CGGACCTGGA GAGACGAGCT TTAAACCTGC TGGAGCACTT CCAGCATCGC 
CAACAGTGCG AGGTGGAGAT TGAAGGTGAC CTGTGCTCCT GCCCCTTTGC CACACCAGAG 
GAGAGCAGTT TGGAGGATGA ATTCTCCCCC TCACATTTCA TGTCCTTCAG TCCTTCTATA 
GTTGCTGAGC AGTTAACAGT CATGGATGCG GAGCTGTTTA AGAGGGTTGT TCCATATCAT 
TGTTTAGGGG GCATATGGTC CCAGAGAGAT AAAAAGGGCA AAGAGCACCT GGCACCAACC 
ATCCGTGCTA CTGTTTCTCA GTTCAACAGT GTGACCAACT GTGTGATCGC CACCTGCCTG 
GACAACAGGT CTCTCAGACC TTTCCAAAGA GCCAAGCGCA TTGAACATTG GATAGAAGTT 
GCCAGGAAAT GCCGTATCCT GAAGAGCTTC TCCTCTCTGA AAGCCATCCT GTCTGCCTTG 
CAGAGTAATG CCATCCACAG GCTGAGAAGG GCCTGGGATG ATGTTTCACG GGAAAGCTAT 
CGAACTTTCC AAGAGCTGTC TGAAATCTTC TCAGACGACA ATAATTACTC ACTCAGCAGA 
GAGCTTCTCA TTAAGGAGGG CACCTCTAAA TCTGCCATAG TCGAGATAAA CCACAAAGGA 
GCCCAGAGGA GACACCAGCA GCAAAGAGAC ATGGGTGTTG TGCAAGGAAC GATTCCATAC 
CTTGGCACTT TTCTGACTGA CCTCGTCATG TTGGATACTG CTATGAAAGA CCATCTGGAG 
GTCGGACTGA TTAACTTTGA GAAGAGAAGA AAGGAATTTG AAGTGATCGC TCAGATCAAG 
TTGCTGCAGC TGACCTGCAA TTATTACAAC TTCAGCAGAA ACCAGCGCTT TATTGACTGG 
TTTAAGAGAG TGGAGAAACT CACAGAGACC GAGAGCTACT CTATGTCGTG TGATATCGAG 
CCGCCGTCTG AGTCGTTGTG CAAAAAGAAT GGAGGCATCA TGAAGCGCAT GAGCGAGGAG 
TCGGGCTACA GCAGCGCTGC GTCACACTCC AAGTCCTTTG AGCAGCCGCG ATTAAGCCAG 
TTCAAAGACT GCTTTAAAGA TGGAGCTGAT TCCCTCAGCC TCACCTCTGC AGGATCCAGT 
GGCTCAGATG CAGACGAGAG TCTCAGCCTG CTCAACTCTC CTGAATCAGG CAGTCAGAGA 
ACATCCACAC CAACTGTGAA ACACTCATCA ACCTCAGAAA CAGGAGAGCT CCCGTCTGAT 
TCCTCAACCA AGCTCTGGGA ATCACCCACC CCATCATCTC TGGAGGCGTC TGGATCAGGC 
TTGTGTTTAG AGTCAGGCTG CAGCAGCTCC ACATCTTCCT CCTCTTCCTC CTCGTCCTCA 
GTCTCAGCCT TCACAGGACG GACCTTCAAC TTTCACAAGC GCTCAGTCTC TGGCATCTCA 
GACTACTCGT CTCTCAGTCT GCCGCTCTAC AACCAGCAGG CAAACGACTG CTGCATCATC 
AGAGTCAGCC TGGACTCAGA AAACGGCAAC ATGTACAAAA GTATCCTGGT CACTAGTCAG 
GACAAAACCC CTGGGGTCAT TAGGAAAGCC ATGGCCAAAC ACAACCTGGA CAATGACAAA 
TCTGAAGACT ATGAATTGTT ACAGAGGGTC TCAAAGCATA AAGAACTCAG AATCCCAGAT 
AATGGAAACG TTTTCTACGC CATGAACTCA ACTGCCAACT ACGACTTCTT GTTGAGAAAG 
CGTGGCGTCC CAAAAACCTG CCGCTCGAAA AGCTCCCCGA GCCTGACTCT ACCACGCATG 
AAGCAGAAAG GCTTCAAAAT CCCCAAGAGC TTCTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Mutagenesis Screen Identifies agtpbp1 and eps15L1 as Essential for T lymphocyte Development in Zebrafish.
PloS one10(7)e0131908(2015)
Seiler C,Gebhart N,Zhang Y,Shinton SA,Li YS,Ross NL,Liu X,Li Q,Bilbee AN,Varshney GK,LaFave MC,Burgess SM,Balciuniene J,Balciunas D,Hardy RR,Kappes DJ,Wiest DL,Rhodes J

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si:ch211-251j10.3 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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