ryr1a cDNA ORF clone, Danio rerio(zebrafish)

The following ryr1a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ryr1a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa145153 XM_021472316.1
Latest version!
Danio rerio ryanodine receptor 1a (skeletal) (ryr1a), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa145154 XM_021472317.1
Latest version!
Danio rerio ryanodine receptor 1a (skeletal) (ryr1a), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa145155 XM_021472318.1
Latest version!
Danio rerio ryanodine receptor 1a (skeletal) (ryr1a), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa145156 XM_021472319.1
Latest version!
Danio rerio ryanodine receptor 1a (skeletal) (ryr1a), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa145157 XM_021472320.1
Latest version!
Danio rerio ryanodine receptor 1a (skeletal) (ryr1a), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa147734 XM_021479405.1
Latest version!
Danio rerio ryanodine receptor 1a (skeletal) (ryr1a), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa47274 XM_009305502.3
Latest version!
Danio rerio ryanodine receptor 1a (skeletal) (ryr1a), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
View Old Accession Versions >>
ODa47274 XM_009305502.2 Danio rerio ryanodine receptor 1a (skeletal) (ryr1a), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa145153
    Clone ID Related Accession (Same CDS sequence) XM_021472316.1
    Accession Version XM_021472316.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8793bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product ryanodine receptor 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394792.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    ATGATCTACT GGGCTCAAGA GTCTTTCATC CAGAACCCTG AGCTGGTGCG CCTCATGTTT 
    AGTCTGCTAC ACCGGCAGTA TGACGCTTTG GGTGAGCTGA TTCGAGCTTT GCCCAAAGCT
    TATATCATTA ATGCTGTTTC TATACAAGAC ACAATGGAGC TCCTGGAGTG TCTGGGACAG
    ATACGCTCCT TGCTCATCGT TCAGATGGGA CCAGAGGAAG AGAGGCTGAT GATCCAGAGT
    ATCGGAAATA TTATGAGTAA CAGGGTATTT TACCAGCACC CCAACTTGAT GAGAGCTCTG
    GGAATGCATG AGACAGTCAT GGAGGTTATG GTGAACGTAC TCGGAGGCGG AGATTCCAAG
    GAGATCAGAT TCCCTCGAAT GGTCACCAAC TGCTGTCGTT TCCTGTGTTA TTTCTGTCGT
    ATCAGCAGAC AGAATCAGCG CTCGATGTTT GATCATCTGG GCTACCTCCT GCAGAACAGT
    GGCATTGGGC TGGGAATGCG TGGCTCCACT CCTCTTGATG TTGCTGCTGC TTCCTGTATC
    GATAATAATG AGCTTGCCCT GGCTCTCCAA GAGCAAGACC TGGAGATGGT AGTAAAGTAC
    TTGGCAGGCT GTGGTCTGCA GAGCTGCCCT ATGCTCTTGT CAAAGGGTTA CCCGGATATT
    GGATGGAACC CAGTTGGAGG AGAAAAGTAT CTGGATTTCC TGCGCTTTGC TGTGTTTGTG
    AATGGAGAGA GTGTGGAGGA GAACGCTAAT GTGGTGGTCC GGCTGCTGAT CAGGAGGCCT
    GAGTGCTTTG GTCCTGCACT GAGAGGAGAA GGGGGCAATG GTCTGCTGGC TGCCATTGAA
    GAAGCCATCA AGATCTCAGA GGACCCTGCA AGAGATGGAC CCACTGTTAA AAAAGACAGA
    CGCTTCCCGA TGTTTGGAGG TGAAGAGCAG CATGAGGAGA ACAGAGTGCA CCTAGGAAAT
    GCCATCATGT CATTTTACTC AGCTCTTATT GATCTGCTGG GTCGCTGTGC TCCTGAGATG
    CATTTGATCC AGGCTGGTAA AGGTGAAGCT TTGAGAATCA GGGCTATTCT TCGGTCGCTT
    GTGCCCATTG AGGATCTGGT CGGTGTGATC AGCTTGTCAG TCCAGATTCC TGCTTTTGGA
    AAAGATAACA GCATTATTGA GCCGAAGATG TCTGCTAGTT TTGTTCCTGA TCATAAAGCA
    CCAATGGTCC TGTTCCTGGA CAGAGTTTAT GGCATCGACA ATCAGGACTT CCTATTGCAT
    GTACTAGAAG TGGGTTTCCT GCCTGATATG AGGGCGGCAG CCTCCTTAGA CACAGCTGCA
    TTCAGCACTA CAGAAATGGC CTTGGCTCTG AACCGTTACC TGTGCTCCGC CGTGCTTCCT
    CTCATCACTA AATGTGCTCC TTTGTTCGCC GGGACGGACC ACCGAGCCAT CATGATCGAC
    TCCATGCTTC ACACCATCTA CAGGCTGTCT CGAGGACGCT CGCTAACTAA AGCACAAAGA
    GATGTTATTG AGGAATGCCT CATGTCTTTG TGCAGGTATC TGCGCCCTTC CATGCTCCAG
    CACCTTCTCA GGCGGCTAGT ATTTGATGTG CCTATTCTGA ATGAGTATGC CAAAATGCCC
    CTTAAGCTGC TTACCAATCA TTATGAGCGT TGTTGGAAAT ATTATTGCTT GCCTAATGGC
    TGGGCTAACT TTGGCGTATC ATCAGAAGAG GAGCTTCATC TGACCCGTAA ACTCTTTTGG
    GGGATCTTTG AATCTCTGGC TCACAAGAAA TTCGATGCAG AGCTGTTCAA AATCGCCATG
    CCTTGTATAT GTGCGATCGC TGGTGCAATT CCTCCTGATT ATGTGGACGC CAGCTACTCC
    TCCAAAACCG AAAAAAAGGC TTCTGTTGAT GCTGAAGGGA ACTTTGACCC CAAACCTGTT
    GATACCACTA ACACTATAAT TCCCGAGAAG TTGGACGGGT TCATCAACAG ATATGCAGAA
    TATACGCACG ACAGATGGGC TTTTGAAAAA ATTCAAAACA ACTGGACATT TGGTGAGGTG
    ATGGATGAAA ATGCCAAGAC TCATCCCATG CTCAGACCTT ACAAAACATT CTCTGAAAAG
    GATAAAGAAA TCTACCGCTG GCCTATTAAA GAGTCCATCA AGGCCATGAT TGCCTGGGAG
    TGGACACTGG ACAAAGCCAG AGATGGAGAG GATGAGAAAG TTGAGAAGAA AACAGCCCGT
    AAGATCTCAC AGACTGCACA GGCAACCTAT GACCCCAGTC ATGGCTATAG CCCCCAACCT
    ATTGATATCT CAGGCATGAC ACTGTCCAGA GAGCTACAGT CCATGGCAGA GCAGCTCGCA
    GAAAACTACC ACAACACCTG GGGCCGTAAG AAGAAAATGG AATTACAAGC AAAAGGAGGA
    GGTACACACC CTTTGCTTGT GCCTTATGAC ACACTGACGG CTAAAGAAAA GGCACGAGAT
    AGAGAGAAAG CGTATGAGCT ACTCAAGTTT CTGCAGCTCA ATGGATATGC TGTGACAAGA
    GGGCTCAAAG ACATGGAAAG TGACATTTCA TCTATCGAGA AGCGTTTTGC GTATGGCTTC
    CTACAGAAGC TTCTGAAGTG GATGGACATT GCTCAGGAGT TCATAGCACA TCTTGAGGCG
    GTTGTGAGCA GCGGTCGTGT GGAAAAGTCA CCACATGAGC AAGAAATCAA GTTCTTTGCC
    AAGATTCTTT TGCCTTTGAT AAATCAGTAT TTTAAAAACC ACTGCCTTTA CTTCCTCTCC
    ACACCGGCCA AAATCCTAGG CAGCGGCGGA CACGCCTCAA ACAAGGAAAA GGAGATGATT
    GCAAGCATTT TCTGTAAGAT GTCTGCCCTG GTGCGACACA GAGTGTTTCT CTTTGGCACG
    GATGCGCCTG CTATTGTCAA CTGCCTTCAT ATCCTCGCAC GTTCGCTTGA TGCCAGGACG
    GTGATGAAAT CCGGGCCTGA GATTGTGAAA GCAGGACTGC GTTTGTTCTT TGAGAGTGCA
    GCTGATGACA TTGAAAAGAT GGTGGAGAAT CTCAAACTTG GCAAAGTGTC CAAAGGCAAT
    CAGCCAGTGA AAGGTGTGTC CCAGAATATA AACTACACCA CAACGGCTTT GCTTCCTGTC
    CTCACATCAC TGTTTGACCA CATCGCTCAA CACCAATTCG GAGATGATGT TATCCTGGAT
    GATTTGCAGA TGTCCTGCTA CCGAATCATG TGTAGCATCT ACTCTCTTGG GACTGTAAAA
    ACTCCACATG CAGAGAGGCA TAGACCAGCC CTTGGAGAAT GTCTCGCTCA TCTCGCTGCA
    GCAATGCCTG TTGCCTACCT GGAGCCGCAT CTTAATGAAT ACAATGCCTT TTCAGTCTAC
    ACTACCAAGA CACCGAGAGA AAGAGCTATC CTTGGCCTTC CAAACCAAGT TCAAGAGCTG
    TGTTTGGACA TTCCTGAGCT TGACGTTTTA CTTAAGGAGA TCGGAGACCT GGCTGAATCG
    GGAGCTCGAT ACACTGAAAT GCCACATGTG ATTGAGGTCA CTCTGCCCAT GCTGTGTAAC
    TATCTGCCCC GCTGGTGGGA GAGAGGACTG GAGAACTTCC CGGATCTGGA GGGTAAGCTG
    TGCACCGACG TCACCTCTGA ATCCCTCAAC CAGCTTTTGG GAAGCATCAT GAAAATCGTG
    GTCAACAACT TGGGAATCGA CGAAGCCTCC TGGATGAAGA GACTTGCAGT GTTCTCCCAA
    CCCATTGTGA GCCGAGCCAA ACCAGAGATG CTCAAATCCC ACTTCATCCC CACCATGGAG
    AAGCTGAAGA AGCGAACGTC TAAAGTTGTA GCCGAGGAGG AGCATCTGCG TTTGGAGGGA
    AAGTCTGAGG GAGATGAGGA AGAGGGAACC ATCAGAGACG AGTTTGCAGT TCTCTGTAGA
    GACCTCTATG CTCTATATCC TCTTCTCATC CGCTATGTGG ATAACAACAG AGCAAGATGG
    CTGACGTTTC GAGACCCAGA TGCTGAGGAG TTGTTCCGCA TGGTGGGAGA GGTCTTCATC
    TTCTGGTCAA AATCTCACAA TTTCAAGCGA GAGGAGCAAA ACTTTGTGGT GATGAACGAA
    ATCAACAATA TGTCCTTTCT CACTGCTGAC AGTAAGAGCA AGATGAGCAA GGCTGGAGGG
    TCGGATGTGG AGCGTACCAA GAAAAAGAGA CGGGGGGATC GATACTCTGT GCAAACGTCC
    CTCATTGTGG CCGCACTGAA GAAAATGCTT CCCATTGGAC TTAACATGTG TTCTCCTGCA
    GACCAGGAGC TGATTAATCT GGCAAAGACA CGTTATTCTC TGAAAGACAC AGATGAGGAG
    GTCAGAGAGT TCTTGCAAAA TAACCTGCAC CTTCAGGGAA AGGTTGATAA CCCATCTATG
    AGGTGGCAGA TGGCACTGTA TAAGGAAATG GCAGGAAAGG CTGAAGATGC AGATGCCCCA
    GAGAATGTGG TCAAGAGGGT TCAGGAGGTC TCTGCGGTCC TTTACCACAT TGAAGTGACG
    GAGCACCCTT TCAAGTCCAA AAAGATGGTT TGGCACAAAC TGCTGTCGAA GCAGCGGCGT
    AGAGCGGTGG TGGCCTGTTT CAGGATGACT CCACTTTACA ACTTGCCCAG ACACAGAGCA
    TCTAATATGT TCCTGGATGG GTACAAGCGC AACTGGATAA GAAATGAAGG CTATTCCTTT
    GAGGACAGAA TGATTGATGA CCTGTCTAAA GCATTGGAGG AGGAAGAAGA GGAGGAAGAG
    GAAAAGGAGA CAAAGCCAGA CCCTCTTCAT CAGCTCATCC TTCACTTTAG TCGCACAGCA
    CTCACTGAGA AAAGTAAACT GGATATAGAT CACCTATACA TGGCATATGC TGATATCATG
    GCTAAGAGTT GCCACATGGA TGAAGAGGAT GAGGGTGGAG AGGAGGAGAA TGAGGGTGGA
    GAGGAGGAGA TGTCCTTTGA GGTGCGACAG ACAGAGATGG AAAAGGAAAT GGAAAAGCAA
    AGACTGCTCT ACCAGCAGTC GCGTCTACAC AACCGAGGGG CTGCTGAAAT GGTCCTGCAG
    ATGATCAGTG CCTGCAAGGG TGAGACTGGT TGTATGGTCT CTTCCACCCT AAAACTTGGC
    ATTTCTATTT TAAATGGCGG GAACTGTGAA GTTCAGCAGA AAATGCTGGA CTATCTGAAA
    GACAAGAAAG ATGTGGGTTT CTTTCTCAGT GTTCAGGCTT TAATGCAGAC CTGCAGTGTT
    TTGGACCTGA ATGCCTTTGA GAGGCAGAAT AAAGCTGAGG GTCTAGGTAT GGTGTCAGAA
    GAGGGTACAA ACATGAAGCT AGAACGGGGT GAGAAGGTCA TGGCTGATGA CGAGTTCACT
    TGCGACCTCT TCCGCTTTCT CCAGCTCCTC TGTGAGGGTC ACAACAATGA CTTCCAAAAT
    TACCTCAGAA CACAGACAGG CAGCACCACC ACCATCAATG TCATTATCTG TACAGTGGAT
    TACCTGCTTA GACTACAGGA GTCCATCAGT GATTTCTACT GGTATTACTC GGGAAAAGAC
    ATCATAGATG AACCAGGAAA GCGAAACTTC TCTAAAGCTA TGACTGTGGC CAAACAGGTT
    TTCAACAGTC TTACCGAGTA TATCCAGGGT CCCTGCACAG GAAACCAGCA GTCCCTGGCT
    CACAGTCGCT TATGGGATGC TGTGGTGGGC TTTCTGCACG TGTTTGCTCA TATGATGATG
    AAACTGGCTC AGGATTCCAG TCAGATTGGT CTTCTGAAAG AGTTGTTGGA CCTTCAGAAA
    GACATGGTGG TCATGTTATT GTCCTTGTTG GAGGGTAATG TTGTAAATGG CACCATTGCT
    CGCCAGATGG TGGACATGCT AGTGGAATCC TCCAGCAATG TAGAAATGAT CCTTAAGTTC
    TTCGACATGT TCCTCAAGCT GAAGGACATT GTTGCGTCTG ACGCGTTTCG TGACTACGTG
    ACCGACCCTA GAGGTTTGAT ATCCAAGAAA GACTTTCAGA AATCCATGGA CAGCCAGAAA
    CAGTACACAC CTTCAGAGAT CCAATTCCTA TTGTCATGCT CTGAGGCTGA TGAGAATGAG
    ATGATCAATT ACGAGGAGTT TGCAAGTCGC TTTCAAGAGC CGGCCAAAGA TATTGGTTTT
    AACATAGCGG TGCTGCTCAC CAACCTCTCC GAACATGTTC CTCACGATAC AAGACTGCAG
    AACTTCCTCG AACAAGCCGA GAGCGTCCTG AACTATTTCC GCCCATTCTT GGGTCGCATC
    GAGATCATGG GCGCCAGCAG GAAAATCGAG AGAATCTACT TCGAGATCAG CGAGGTGAAT
    CGCAAGCAGT GGGAAATGCC TCAAGTCAAA GAATCGAAGA GACAGTTCAT ATTCGACGTA
    GTCAATGAAG GCGGCGAATC GGAGAAGATG GAGCTTTTCG TCAACTTCTG CGAGGACACC
    ATATTCGAGA TGAACATCGC GTCGCAGATT TCAGAACAAG ACGAGGAAGA GAAAGAAGAG
    GATGATATTG AGGAAGCTGA AGGAGGAGAT GCTGGTGATG AAGAGGGGGG TGGCGAGGAG
    GGACAACCAG AATCAAGCTC TGCATTTGCC GATTTCATTC AAAGCATCAT GAATTTTCTG
    GGTATGTTCA CCTTCAGGAA TATTCGCAGA CAGTACCGCA GACTCCGCAA GATGACCATT
    AAGGAGATCG TGGTAGGGCT TGCTACCTTC ATGTGGACGA TTCTCTTGGG CATCCTCCAT
    TTCATTTACA GTGTTTGTAA AGGCTTCTTC CTCCTCATCT GGCACACGCT GTTTGGAGGA
    GGTTTGGTTG AAGGGGCCAA AAACATGACA GTAACTGAGT TACTAGCCAG CATGCCAGAT
    CCCACACAGG ACGAGGTGCA TGGGGACTTA CCTGGAGAAC CGAGGGGAGG AGAGGAACAG
    GAGACTGGAG GGATAATGGA CTCGATGGAT GCTGGAGGGG GAGAGGAAGA GGATGAGGAT
    ATCCAGGAGA AAGATGGTGG AAAGATTCCT GGTATAGATA CACCTGGTGG ACTTGGAGAT
    ATGGGTGATA CAACTCCTGT AGAACCGCCG ACTCCCGAGG GTACCCCTTT GTTGAAGAGG
    AAAGCGCAAC CAGATGAAGC AGGACCTGAA CAGCCTCCTG CTATTGAAGA GCCTCCTCCT
    GAGCCTGAGA AAGCAGACAT TGAGAATGGT GAGAAGGCTG AGAAGGAAGT GGAGGTCAAG
    CCTGAGCCGA TACCAGAAGA ACCGAAACCT CAGAGAGCCA CCAAAGCCAA GAAGGAGAAG
    AAATCAGTCA AGGGTGCAGG ATTTGAGCTC TGGAATGAGC TGGATGTGCA AAGAAATAAA
    TTCATGAACT GCTTATCTCG TAACTTCTAC AACATTCGTT TCCTGGCTCT GTTCCTTGCC
    TTTGCCTTGA ACTTCATTCT TCTCTTCTAC AAGGTATCTG ATACTCCCCC CAGTTCCGAT
    GAATTTGAAG GTTCTGGTAT CTTTGAAGGC TCTGGGCTGT TTGAAGGCTC TGCGGAGGAT
    TTCGAGGGCT CTGGTGGAGA GGATGGAGGT GATGATGAGG ATGAAGAAGG ACCTGTGTAT
    TATTTTCTGG AAGAAAGCAC GGGATACATG CAGCCCACTC TGTCCTTCCT GGCCATTTTG
    CACACCGTCA TTGCTTTCAT ATCTATCATT GGATACAACT GCCTCAAGAT TCCACTGGTC
    ATCTTTAAGA GAGAGAAAGA GTTGGCGAGA AAACTGGAGT TTGATGGTCT TTACATCACT
    GAACAGCCGG CAGATGATGA CATTAAAGGC CAGTGGGACA GACTGGTGTT AAACACTCCC
    TCTTTTCCCA CCAATTACTG GGACAAATTT GTGAAGCGAA AGGTTCTGGA TAAATATGGA
    GATATTTATG GACGAGAGAG AATCGCAGAG CTGCTGGGTA TGGATCTGGC ATCTCTGGAT
    GTCAGCAAAC AACAGACAGA CAAGAAACCA GAAGAGCCAG ACAACTCTAT GCTTGCATGG
    TTCACTTCCA TTGACATCAA GTATCAGATC TGGAAATTTG GAGTTGTGTT CACAGACAAT
    ACTTTCCTCT ACCTGGTGTG GTACACAGTG ATGTCCCTTC TTGGACATTA CAACAACTTT
    TTCTTTGCTT GTCACCTGCT GGACATCGCA ATGGGTGTCA AGACACTGCG TACAATTCTT
    TCCTCCGTCA CACACAATGG AAAACAGCTG ATGATGACTG TGGGTTTGCT GGCTGTGGTT
    GTGTACCTCT ACACTGTGGT GGCCTTCAAC TTCTTCAGGA AGTTCTACAA TAAGAGCGAG
    GATGAGGATG AGCCGGACAT GAAGTGTGAC GACATGATGA CGTGTTATTT GTTCCACATG
    TATGTGGGTG TACGGGCAGG TGGAGGTATA GGAGATGAGA TCGAGGACCC TGCTGGAGAT
    GAGTATGAGC TCTATCGGGT GGTCTTCGAC ATCACCTTCT TCTTCTTTGT CATCGTCATC
    CTGCTGGCCA TCATTCAGGG TCTGATCATT GATGCCTTTG GTGAACTGAG AGACCAGCAG
    GAGCAAGTGA AAGAGGACAT GGAGACCAAA TGCTTTATAT GTGGCATTGG GAGCGACTAC
    TTTGATACAA CGCCCCATGG CTTTGAGACC CATACACTTG AGGAACACAA CCTGGCCAAT
    TACATGTTTT TCCTGATGTA CTTAATCAAC AAGGATGAAA CAGAGCACAC TGGCCAGGAA
    TCATACGTGT GGAAGATGTA CCAGGAGAGA TGCTGGGATT TCTTCCCTGC CGGAGACTGC
    TTCAGAAAGC AATATGAAGA CCAGCTTGGA TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021327991.1
    CDS162..8954
    Translation

    Target ORF information:

    RefSeq Version XM_021472316.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio ryanodine receptor 1a (skeletal) (ryr1a), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021472316.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    ATGATCTACT GGGCTCAAGA GTCTTTCATC CAGAACCCTG AGCTGGTGCG CCTCATGTTT 
    AGTCTGCTAC ACCGGCAGTA TGACGCTTTG GGTGAGCTGA TTCGAGCTTT GCCCAAAGCT
    TATATCATTA ATGCTGTTTC TATACAAGAC ACAATGGAGC TCCTGGAGTG TCTGGGACAG
    ATACGCTCCT TGCTCATCGT TCAGATGGGA CCAGAGGAAG AGAGGCTGAT GATCCAGAGT
    ATCGGAAATA TTATGAGTAA CAGGGTATTT TACCAGCACC CCAACTTGAT GAGAGCTCTG
    GGAATGCATG AGACAGTCAT GGAGGTTATG GTGAACGTAC TCGGAGGCGG AGATTCCAAG
    GAGATCAGAT TCCCTCGAAT GGTCACCAAC TGCTGTCGTT TCCTGTGTTA TTTCTGTCGT
    ATCAGCAGAC AGAATCAGCG CTCGATGTTT GATCATCTGG GCTACCTCCT GCAGAACAGT
    GGCATTGGGC TGGGAATGCG TGGCTCCACT CCTCTTGATG TTGCTGCTGC TTCCTGTATC
    GATAATAATG AGCTTGCCCT GGCTCTCCAA GAGCAAGACC TGGAGATGGT AGTAAAGTAC
    TTGGCAGGCT GTGGTCTGCA GAGCTGCCCT ATGCTCTTGT CAAAGGGTTA CCCGGATATT
    GGATGGAACC CAGTTGGAGG AGAAAAGTAT CTGGATTTCC TGCGCTTTGC TGTGTTTGTG
    AATGGAGAGA GTGTGGAGGA GAACGCTAAT GTGGTGGTCC GGCTGCTGAT CAGGAGGCCT
    GAGTGCTTTG GTCCTGCACT GAGAGGAGAA GGGGGCAATG GTCTGCTGGC TGCCATTGAA
    GAAGCCATCA AGATCTCAGA GGACCCTGCA AGAGATGGAC CCACTGTTAA AAAAGACAGA
    CGCTTCCCGA TGTTTGGAGG TGAAGAGCAG CATGAGGAGA ACAGAGTGCA CCTAGGAAAT
    GCCATCATGT CATTTTACTC AGCTCTTATT GATCTGCTGG GTCGCTGTGC TCCTGAGATG
    CATTTGATCC AGGCTGGTAA AGGTGAAGCT TTGAGAATCA GGGCTATTCT TCGGTCGCTT
    GTGCCCATTG AGGATCTGGT CGGTGTGATC AGCTTGTCAG TCCAGATTCC TGCTTTTGGA
    AAAGATAACA GCATTATTGA GCCGAAGATG TCTGCTAGTT TTGTTCCTGA TCATAAAGCA
    CCAATGGTCC TGTTCCTGGA CAGAGTTTAT GGCATCGACA ATCAGGACTT CCTATTGCAT
    GTACTAGAAG TGGGTTTCCT GCCTGATATG AGGGCGGCAG CCTCCTTAGA CACAGCTGCA
    TTCAGCACTA CAGAAATGGC CTTGGCTCTG AACCGTTACC TGTGCTCCGC CGTGCTTCCT
    CTCATCACTA AATGTGCTCC TTTGTTCGCC GGGACGGACC ACCGAGCCAT CATGATCGAC
    TCCATGCTTC ACACCATCTA CAGGCTGTCT CGAGGACGCT CGCTAACTAA AGCACAAAGA
    GATGTTATTG AGGAATGCCT CATGTCTTTG TGCAGGTATC TGCGCCCTTC CATGCTCCAG
    CACCTTCTCA GGCGGCTAGT ATTTGATGTG CCTATTCTGA ATGAGTATGC CAAAATGCCC
    CTTAAGCTGC TTACCAATCA TTATGAGCGT TGTTGGAAAT ATTATTGCTT GCCTAATGGC
    TGGGCTAACT TTGGCGTATC ATCAGAAGAG GAGCTTCATC TGACCCGTAA ACTCTTTTGG
    GGGATCTTTG AATCTCTGGC TCACAAGAAA TTCGATGCAG AGCTGTTCAA AATCGCCATG
    CCTTGTATAT GTGCGATCGC TGGTGCAATT CCTCCTGATT ATGTGGACGC CAGCTACTCC
    TCCAAAACCG AAAAAAAGGC TTCTGTTGAT GCTGAAGGGA ACTTTGACCC CAAACCTGTT
    GATACCACTA ACACTATAAT TCCCGAGAAG TTGGACGGGT TCATCAACAG ATATGCAGAA
    TATACGCACG ACAGATGGGC TTTTGAAAAA ATTCAAAACA ACTGGACATT TGGTGAGGTG
    ATGGATGAAA ATGCCAAGAC TCATCCCATG CTCAGACCTT ACAAAACATT CTCTGAAAAG
    GATAAAGAAA TCTACCGCTG GCCTATTAAA GAGTCCATCA AGGCCATGAT TGCCTGGGAG
    TGGACACTGG ACAAAGCCAG AGATGGAGAG GATGAGAAAG TTGAGAAGAA AACAGCCCGT
    AAGATCTCAC AGACTGCACA GGCAACCTAT GACCCCAGTC ATGGCTATAG CCCCCAACCT
    ATTGATATCT CAGGCATGAC ACTGTCCAGA GAGCTACAGT CCATGGCAGA GCAGCTCGCA
    GAAAACTACC ACAACACCTG GGGCCGTAAG AAGAAAATGG AATTACAAGC AAAAGGAGGA
    GGTACACACC CTTTGCTTGT GCCTTATGAC ACACTGACGG CTAAAGAAAA GGCACGAGAT
    AGAGAGAAAG CGTATGAGCT ACTCAAGTTT CTGCAGCTCA ATGGATATGC TGTGACAAGA
    GGGCTCAAAG ACATGGAAAG TGACATTTCA TCTATCGAGA AGCGTTTTGC GTATGGCTTC
    CTACAGAAGC TTCTGAAGTG GATGGACATT GCTCAGGAGT TCATAGCACA TCTTGAGGCG
    GTTGTGAGCA GCGGTCGTGT GGAAAAGTCA CCACATGAGC AAGAAATCAA GTTCTTTGCC
    AAGATTCTTT TGCCTTTGAT AAATCAGTAT TTTAAAAACC ACTGCCTTTA CTTCCTCTCC
    ACACCGGCCA AAATCCTAGG CAGCGGCGGA CACGCCTCAA ACAAGGAAAA GGAGATGATT
    GCAAGCATTT TCTGTAAGAT GTCTGCCCTG GTGCGACACA GAGTGTTTCT CTTTGGCACG
    GATGCGCCTG CTATTGTCAA CTGCCTTCAT ATCCTCGCAC GTTCGCTTGA TGCCAGGACG
    GTGATGAAAT CCGGGCCTGA GATTGTGAAA GCAGGACTGC GTTTGTTCTT TGAGAGTGCA
    GCTGATGACA TTGAAAAGAT GGTGGAGAAT CTCAAACTTG GCAAAGTGTC CAAAGGCAAT
    CAGCCAGTGA AAGGTGTGTC CCAGAATATA AACTACACCA CAACGGCTTT GCTTCCTGTC
    CTCACATCAC TGTTTGACCA CATCGCTCAA CACCAATTCG GAGATGATGT TATCCTGGAT
    GATTTGCAGA TGTCCTGCTA CCGAATCATG TGTAGCATCT ACTCTCTTGG GACTGTAAAA
    ACTCCACATG CAGAGAGGCA TAGACCAGCC CTTGGAGAAT GTCTCGCTCA TCTCGCTGCA
    GCAATGCCTG TTGCCTACCT GGAGCCGCAT CTTAATGAAT ACAATGCCTT TTCAGTCTAC
    ACTACCAAGA CACCGAGAGA AAGAGCTATC CTTGGCCTTC CAAACCAAGT TCAAGAGCTG
    TGTTTGGACA TTCCTGAGCT TGACGTTTTA CTTAAGGAGA TCGGAGACCT GGCTGAATCG
    GGAGCTCGAT ACACTGAAAT GCCACATGTG ATTGAGGTCA CTCTGCCCAT GCTGTGTAAC
    TATCTGCCCC GCTGGTGGGA GAGAGGACTG GAGAACTTCC CGGATCTGGA GGGTAAGCTG
    TGCACCGACG TCACCTCTGA ATCCCTCAAC CAGCTTTTGG GAAGCATCAT GAAAATCGTG
    GTCAACAACT TGGGAATCGA CGAAGCCTCC TGGATGAAGA GACTTGCAGT GTTCTCCCAA
    CCCATTGTGA GCCGAGCCAA ACCAGAGATG CTCAAATCCC ACTTCATCCC CACCATGGAG
    AAGCTGAAGA AGCGAACGTC TAAAGTTGTA GCCGAGGAGG AGCATCTGCG TTTGGAGGGA
    AAGTCTGAGG GAGATGAGGA AGAGGGAACC ATCAGAGACG AGTTTGCAGT TCTCTGTAGA
    GACCTCTATG CTCTATATCC TCTTCTCATC CGCTATGTGG ATAACAACAG AGCAAGATGG
    CTGACGTTTC GAGACCCAGA TGCTGAGGAG TTGTTCCGCA TGGTGGGAGA GGTCTTCATC
    TTCTGGTCAA AATCTCACAA TTTCAAGCGA GAGGAGCAAA ACTTTGTGGT GATGAACGAA
    ATCAACAATA TGTCCTTTCT CACTGCTGAC AGTAAGAGCA AGATGAGCAA GGCTGGAGGG
    TCGGATGTGG AGCGTACCAA GAAAAAGAGA CGGGGGGATC GATACTCTGT GCAAACGTCC
    CTCATTGTGG CCGCACTGAA GAAAATGCTT CCCATTGGAC TTAACATGTG TTCTCCTGCA
    GACCAGGAGC TGATTAATCT GGCAAAGACA CGTTATTCTC TGAAAGACAC AGATGAGGAG
    GTCAGAGAGT TCTTGCAAAA TAACCTGCAC CTTCAGGGAA AGGTTGATAA CCCATCTATG
    AGGTGGCAGA TGGCACTGTA TAAGGAAATG GCAGGAAAGG CTGAAGATGC AGATGCCCCA
    GAGAATGTGG TCAAGAGGGT TCAGGAGGTC TCTGCGGTCC TTTACCACAT TGAAGTGACG
    GAGCACCCTT TCAAGTCCAA AAAGATGGTT TGGCACAAAC TGCTGTCGAA GCAGCGGCGT
    AGAGCGGTGG TGGCCTGTTT CAGGATGACT CCACTTTACA ACTTGCCCAG ACACAGAGCA
    TCTAATATGT TCCTGGATGG GTACAAGCGC AACTGGATAA GAAATGAAGG CTATTCCTTT
    GAGGACAGAA TGATTGATGA CCTGTCTAAA GCATTGGAGG AGGAAGAAGA GGAGGAAGAG
    GAAAAGGAGA CAAAGCCAGA CCCTCTTCAT CAGCTCATCC TTCACTTTAG TCGCACAGCA
    CTCACTGAGA AAAGTAAACT GGATATAGAT CACCTATACA TGGCATATGC TGATATCATG
    GCTAAGAGTT GCCACATGGA TGAAGAGGAT GAGGGTGGAG AGGAGGAGAA TGAGGGTGGA
    GAGGAGGAGA TGTCCTTTGA GGTGCGACAG ACAGAGATGG AAAAGGAAAT GGAAAAGCAA
    AGACTGCTCT ACCAGCAGTC GCGTCTACAC AACCGAGGGG CTGCTGAAAT GGTCCTGCAG
    ATGATCAGTG CCTGCAAGGG TGAGACTGGT TGTATGGTCT CTTCCACCCT AAAACTTGGC
    ATTTCTATTT TAAATGGCGG GAACTGTGAA GTTCAGCAGA AAATGCTGGA CTATCTGAAA
    GACAAGAAAG ATGTGGGTTT CTTTCTCAGT GTTCAGGCTT TAATGCAGAC CTGCAGTGTT
    TTGGACCTGA ATGCCTTTGA GAGGCAGAAT AAAGCTGAGG GTCTAGGTAT GGTGTCAGAA
    GAGGGTACAA ACATGAAGCT AGAACGGGGT GAGAAGGTCA TGGCTGATGA CGAGTTCACT
    TGCGACCTCT TCCGCTTTCT CCAGCTCCTC TGTGAGGGTC ACAACAATGA CTTCCAAAAT
    TACCTCAGAA CACAGACAGG CAGCACCACC ACCATCAATG TCATTATCTG TACAGTGGAT
    TACCTGCTTA GACTACAGGA GTCCATCAGT GATTTCTACT GGTATTACTC GGGAAAAGAC
    ATCATAGATG AACCAGGAAA GCGAAACTTC TCTAAAGCTA TGACTGTGGC CAAACAGGTT
    TTCAACAGTC TTACCGAGTA TATCCAGGGT CCCTGCACAG GAAACCAGCA GTCCCTGGCT
    CACAGTCGCT TATGGGATGC TGTGGTGGGC TTTCTGCACG TGTTTGCTCA TATGATGATG
    AAACTGGCTC AGGATTCCAG TCAGATTGGT CTTCTGAAAG AGTTGTTGGA CCTTCAGAAA
    GACATGGTGG TCATGTTATT GTCCTTGTTG GAGGGTAATG TTGTAAATGG CACCATTGCT
    CGCCAGATGG TGGACATGCT AGTGGAATCC TCCAGCAATG TAGAAATGAT CCTTAAGTTC
    TTCGACATGT TCCTCAAGCT GAAGGACATT GTTGCGTCTG ACGCGTTTCG TGACTACGTG
    ACCGACCCTA GAGGTTTGAT ATCCAAGAAA GACTTTCAGA AATCCATGGA CAGCCAGAAA
    CAGTACACAC CTTCAGAGAT CCAATTCCTA TTGTCATGCT CTGAGGCTGA TGAGAATGAG
    ATGATCAATT ACGAGGAGTT TGCAAGTCGC TTTCAAGAGC CGGCCAAAGA TATTGGTTTT
    AACATAGCGG TGCTGCTCAC CAACCTCTCC GAACATGTTC CTCACGATAC AAGACTGCAG
    AACTTCCTCG AACAAGCCGA GAGCGTCCTG AACTATTTCC GCCCATTCTT GGGTCGCATC
    GAGATCATGG GCGCCAGCAG GAAAATCGAG AGAATCTACT TCGAGATCAG CGAGGTGAAT
    CGCAAGCAGT GGGAAATGCC TCAAGTCAAA GAATCGAAGA GACAGTTCAT ATTCGACGTA
    GTCAATGAAG GCGGCGAATC GGAGAAGATG GAGCTTTTCG TCAACTTCTG CGAGGACACC
    ATATTCGAGA TGAACATCGC GTCGCAGATT TCAGAACAAG ACGAGGAAGA GAAAGAAGAG
    GATGATATTG AGGAAGCTGA AGGAGGAGAT GCTGGTGATG AAGAGGGGGG TGGCGAGGAG
    GGACAACCAG AATCAAGCTC TGCATTTGCC GATTTCATTC AAAGCATCAT GAATTTTCTG
    GGTATGTTCA CCTTCAGGAA TATTCGCAGA CAGTACCGCA GACTCCGCAA GATGACCATT
    AAGGAGATCG TGGTAGGGCT TGCTACCTTC ATGTGGACGA TTCTCTTGGG CATCCTCCAT
    TTCATTTACA GTGTTTGTAA AGGCTTCTTC CTCCTCATCT GGCACACGCT GTTTGGAGGA
    GGTTTGGTTG AAGGGGCCAA AAACATGACA GTAACTGAGT TACTAGCCAG CATGCCAGAT
    CCCACACAGG ACGAGGTGCA TGGGGACTTA CCTGGAGAAC CGAGGGGAGG AGAGGAACAG
    GAGACTGGAG GGATAATGGA CTCGATGGAT GCTGGAGGGG GAGAGGAAGA GGATGAGGAT
    ATCCAGGAGA AAGATGGTGG AAAGATTCCT GGTATAGATA CACCTGGTGG ACTTGGAGAT
    ATGGGTGATA CAACTCCTGT AGAACCGCCG ACTCCCGAGG GTACCCCTTT GTTGAAGAGG
    AAAGCGCAAC CAGATGAAGC AGGACCTGAA CAGCCTCCTG CTATTGAAGA GCCTCCTCCT
    GAGCCTGAGA AAGCAGACAT TGAGAATGGT GAGAAGGCTG AGAAGGAAGT GGAGGTCAAG
    CCTGAGCCGA TACCAGAAGA ACCGAAACCT CAGAGAGCCA CCAAAGCCAA GAAGGAGAAG
    AAATCAGTCA AGGGTGCAGG ATTTGAGCTC TGGAATGAGC TGGATGTGCA AAGAAATAAA
    TTCATGAACT GCTTATCTCG TAACTTCTAC AACATTCGTT TCCTGGCTCT GTTCCTTGCC
    TTTGCCTTGA ACTTCATTCT TCTCTTCTAC AAGGTATCTG ATACTCCCCC CAGTTCCGAT
    GAATTTGAAG GTTCTGGTAT CTTTGAAGGC TCTGGGCTGT TTGAAGGCTC TGCGGAGGAT
    TTCGAGGGCT CTGGTGGAGA GGATGGAGGT GATGATGAGG ATGAAGAAGG ACCTGTGTAT
    TATTTTCTGG AAGAAAGCAC GGGATACATG CAGCCCACTC TGTCCTTCCT GGCCATTTTG
    CACACCGTCA TTGCTTTCAT ATCTATCATT GGATACAACT GCCTCAAGAT TCCACTGGTC
    ATCTTTAAGA GAGAGAAAGA GTTGGCGAGA AAACTGGAGT TTGATGGTCT TTACATCACT
    GAACAGCCGG CAGATGATGA CATTAAAGGC CAGTGGGACA GACTGGTGTT AAACACTCCC
    TCTTTTCCCA CCAATTACTG GGACAAATTT GTGAAGCGAA AGGTTCTGGA TAAATATGGA
    GATATTTATG GACGAGAGAG AATCGCAGAG CTGCTGGGTA TGGATCTGGC ATCTCTGGAT
    GTCAGCAAAC AACAGACAGA CAAGAAACCA GAAGAGCCAG ACAACTCTAT GCTTGCATGG
    TTCACTTCCA TTGACATCAA GTATCAGATC TGGAAATTTG GAGTTGTGTT CACAGACAAT
    ACTTTCCTCT ACCTGGTGTG GTACACAGTG ATGTCCCTTC TTGGACATTA CAACAACTTT
    TTCTTTGCTT GTCACCTGCT GGACATCGCA ATGGGTGTCA AGACACTGCG TACAATTCTT
    TCCTCCGTCA CACACAATGG AAAACAGCTG ATGATGACTG TGGGTTTGCT GGCTGTGGTT
    GTGTACCTCT ACACTGTGGT GGCCTTCAAC TTCTTCAGGA AGTTCTACAA TAAGAGCGAG
    GATGAGGATG AGCCGGACAT GAAGTGTGAC GACATGATGA CGTGTTATTT GTTCCACATG
    TATGTGGGTG TACGGGCAGG TGGAGGTATA GGAGATGAGA TCGAGGACCC TGCTGGAGAT
    GAGTATGAGC TCTATCGGGT GGTCTTCGAC ATCACCTTCT TCTTCTTTGT CATCGTCATC
    CTGCTGGCCA TCATTCAGGG TCTGATCATT GATGCCTTTG GTGAACTGAG AGACCAGCAG
    GAGCAAGTGA AAGAGGACAT GGAGACCAAA TGCTTTATAT GTGGCATTGG GAGCGACTAC
    TTTGATACAA CGCCCCATGG CTTTGAGACC CATACACTTG AGGAACACAA CCTGGCCAAT
    TACATGTTTT TCCTGATGTA CTTAATCAAC AAGGATGAAA CAGAGCACAC TGGCCAGGAA
    TCATACGTGT GGAAGATGTA CCAGGAGAGA TGCTGGGATT TCTTCCCTGC CGGAGACTGC
    TTCAGAAAGC AATATGAAGA CCAGCTTGGA TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa145154
    Clone ID Related Accession (Same CDS sequence) XM_021472317.1
    Accession Version XM_021472317.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8799bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product ryanodine receptor 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394792.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
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    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    ATGATCTACT GGGCTCAAGA GTCTTTCATC CAGAACCCTG AGCTGGTGCG CCTCATGTTT 
    AGTCTGCTAC ACCGGCAGTA TGACGCTTTG GGTGAGCTGA TTCGAGCTTT GCCCAAAGCT
    TATATCATTA ATGCTGTTTC TATACAAGAC ACAATGGAGC TCCTGGAGTG TCTGGGACAG
    ATACGCTCCT TGCTCATCGT TCAGATGGGA CCAGAGGAAG AGAGGCTGAT GATCCAGAGT
    ATCGGAAATA TTATGAGTAA CAGGGTATTT TACCAGCACC CCAACTTGAT GAGAGCTCTG
    GGAATGCATG AGACAGTCAT GGAGGTTATG GTGAACGTAC TCGGAGGCGG AGATTCCAAG
    GAGATCAGAT TCCCTCGAAT GGTCACCAAC TGCTGTCGTT TCCTGTGTTA TTTCTGTCGT
    ATCAGCAGAC AGAATCAGCG CTCGATGTTT GATCATCTGG GCTACCTCCT GCAGAACAGT
    GGCATTGGGC TGGGAATGCG TGGCTCCACT CCTCTTGATG TTGCTGCTGC TTCCTGTATC
    GATAATAATG AGCTTGCCCT GGCTCTCCAA GAGCAAGACC TGGAGATGGT AGTAAAGTAC
    TTGGCAGGCT GTGGTCTGCA GAGCTGCCCT ATGCTCTTGT CAAAGGGTTA CCCGGATATT
    GGATGGAACC CAGTTGGAGG AGAAAAGTAT CTGGATTTCC TGCGCTTTGC TGTGTTTGTG
    AATGGAGAGA GTGTGGAGGA GAACGCTAAT GTGGTGGTCC GGCTGCTGAT CAGGAGGCCT
    GAGTGCTTTG GTCCTGCACT GAGAGGAGAA GGGGGCAATG GTCTGCTGGC TGCCATTGAA
    GAAGCCATCA AGATCTCAGA GGACCCTGCA AGAGATGGAC CCACTGTTAA AAAAGACAGA
    CGCTTCCCGA TGTTTGGAGG TGAAGAGCAG CATGAGGAGA ACAGAGTGCA CCTAGGAAAT
    GCCATCATGT CATTTTACTC AGCTCTTATT GATCTGCTGG GTCGCTGTGC TCCTGAGATG
    CATTTGATCC AGGCTGGTAA AGGTGAAGCT TTGAGAATCA GGGCTATTCT TCGGTCGCTT
    GTGCCCATTG AGGATCTGGT CGGTGTGATC AGCTTGTCAG TCCAGATTCC TGCTTTTGGA
    AAAGATAACA GCATTATTGA GCCGAAGATG TCTGCTAGTT TTGTTCCTGA TCATAAAGCA
    CCAATGGTCC TGTTCCTGGA CAGAGTTTAT GGCATCGACA ATCAGGACTT CCTATTGCAT
    GTACTAGAAG TGGGTTTCCT GCCTGATATG AGGGCGGCAG CCTCCTTAGA CACAGCTGCA
    TTCAGCACTA CAGAAATGGC CTTGGCTCTG AACCGTTACC TGTGCTCCGC CGTGCTTCCT
    CTCATCACTA AATGTGCTCC TTTGTTCGCC GGGACGGACC ACCGAGCCAT CATGATCGAC
    TCCATGCTTC ACACCATCTA CAGGCTGTCT CGAGGACGCT CGCTAACTAA AGCACAAAGA
    GATGTTATTG AGGAATGCCT CATGTCTTTG TGCAGGTATC TGCGCCCTTC CATGCTCCAG
    CACCTTCTCA GGCGGCTAGT ATTTGATGTG CCTATTCTGA ATGAGTATGC CAAAATGCCC
    CTTAAGCTGC TTACCAATCA TTATGAGCGT TGTTGGAAAT ATTATTGCTT GCCTAATGGC
    TGGGCTAACT TTGGCGTATC ATCAGAAGAG GAGCTTCATC TGACCCGTAA ACTCTTTTGG
    GGGATCTTTG AATCTCTGGC TCACAAGAAA TTCGATGCAG AGCTGTTCAA AATCGCCATG
    CCTTGTATAT GTGCGATCGC TGGTGCAATT CCTCCTGATT ATGTGGACGC CAGCTACTCC
    TCCAAAACCG AAAAAAAGGC TTCTGTTGAT GCTGAAGGGA ACTTTGACCC CAAACCTGTT
    GATACCACTA ACACTATAAT TCCCGAGAAG TTGGACGGGT TCATCAACAG ATATGCAGAA
    TATACGCACG ACAGATGGGC TTTTGAAAAA ATTCAAAACA ACTGGACATT TGGTGAGGTG
    ATGGATGAAA ATGCCAAGAC TCATCCCATG CTCAGACCTT ACAAAACATT CTCTGAAAAG
    GATAAAGAAA TCTACCGCTG GCCTATTAAA GAGTCCATCA AGGCCATGAT TGCCTGGGAG
    TGGACACTGG ACAAAGCCAG AGATGGAGAG GATGAGAAAG TTGAGAAGAA AACAGCCCGT
    AAGATCTCAC AGACTGCACA GGCAACCTAT GACCCCAGTC ATGGCTATAG CCCCCAACCT
    ATTGATATCT CAGGCATGAC ACTGTCCAGA GAGCTACAGT CCATGGCAGA GCAGCTCGCA
    GAAAACTACC ACAACACCTG GGGCCGTAAG AAGAAAATGG AATTACAAGC AAAAGGAGGA
    GGTACACACC CTTTGCTTGT GCCTTATGAC ACACTGACGG CTAAAGAAAA GGCACGAGAT
    AGAGAGAAAG CGTATGAGCT ACTCAAGTTT CTGCAGCTCA ATGGATATGC TGTGACAAGA
    GGGCTCAAAG ACATGGAAAG TGACATTTCA TCTATCGAGA AGCGTTTTGC GTATGGCTTC
    CTACAGAAGC TTCTGAAGTG GATGGACATT GCTCAGGAGT TCATAGCACA TCTTGAGGCG
    GTTGTGAGCA GCGGTCGTGT GGAAAAGTCA CCACATGAGC AAGAAATCAA GTTCTTTGCC
    AAGATTCTTT TGCCTTTGAT AAATCAGTAT TTTAAAAACC ACTGCCTTTA CTTCCTCTCC
    ACACCGGCCA AAATCCTAGG CAGCGGCGGA CACGCCTCAA ACAAGGAAAA GGAGATGATT
    GCAAGCATTT TCTGTAAGAT GTCTGCCCTG GTGCGACACA GAGTGTTTCT CTTTGGCACG
    GATGCGCCTG CTATTGTCAA CTGCCTTCAT ATCCTCGCAC GTTCGCTTGA TGCCAGGACG
    GTGATGAAAT CCGGGCCTGA GATTGTGAAA GCAGGACTGC GTTTGTTCTT TGAGAGTGCA
    GCTGATGACA TTGAAAAGAT GGTGGAGAAT CTCAAACTTG GCAAAGTGTC CAAAGGCAAT
    CAGCCAGTGA AAGGTGTGTC CCAGAATATA AACTACACCA CAACGGCTTT GCTTCCTGTC
    CTCACATCAC TGTTTGACCA CATCGCTCAA CACCAATTCG GAGATGATGT TATCCTGGAT
    GATTTGCAGA TGTCCTGCTA CCGAATCATG TGTAGCATCT ACTCTCTTGG GACTGTAAAA
    ACTCCACATG CAGAGAGGCA TAGACCAGCC CTTGGAGAAT GTCTCGCTCA TCTCGCTGCA
    GCAATGCCTG TTGCCTACCT GGAGCCGCAT CTTAATGAAT ACAATGCCTT TTCAGTCTAC
    ACTACCAAGA CACCGAGAGA AAGAGCTATC CTTGGCCTTC CAAACCAAGT TCAAGAGCTG
    TGTTTGGACA TTCCTGAGCT TGACGTTTTA CTTAAGGAGA TCGGAGACCT GGCTGAATCG
    GGAGCTCGAT ACACTGAAAT GCCACATGTG ATTGAGGTCA CTCTGCCCAT GCTGTGTAAC
    TATCTGCCCC GCTGGTGGGA GAGAGGACTG GAGAACTTCC CGGATCTGGA GGGTAAGCTG
    TGCACCGACG TCACCTCTGA ATCCCTCAAC CAGCTTTTGG GAAGCATCAT GAAAATCGTG
    GTCAACAACT TGGGAATCGA CGAAGCCTCC TGGATGAAGA GACTTGCAGT GTTCTCCCAA
    CCCATTGTGA GCCGAGCCAA ACCAGAGATG CTCAAATCCC ACTTCATCCC CACCATGGAG
    AAGCTGAAGA AGCGAACGTC TAAAGTTGTA GCCGAGGAGG AGCATCTGCG TTTGGAGGGA
    AAGTCTGAGG GAGATGAGGA AGAGGGAACC ATCAGAGACG AGTTTGCAGT TCTCTGTAGA
    GACCTCTATG CTCTATATCC TCTTCTCATC CGCTATGTGG ATAACAACAG AGCAAGATGG
    CTGACGTTTC GAGACCCAGA TGCTGAGGAG TTGTTCCGCA TGGTGGGAGA GGTCTTCATC
    TTCTGGTCAA AATCTCACAA TTTCAAGCGA GAGGAGCAAA ACTTTGTGGT GATGAACGAA
    ATCAACAATA TGTCCTTTCT CACTGCTGAC AGTAAGAGCA AGATGAGCAA GGCTGGAGGG
    TCGGATGTGG AGCGTACCAA GAAAAAGAGA CGGGGGGATC GATACTCTGT GCAAACGTCC
    CTCATTGTGG CCGCACTGAA GAAAATGCTT CCCATTGGAC TTAACATGTG TTCTCCTGCA
    GACCAGGAGC TGATTAATCT GGCAAAGACA CGTTATTCTC TGAAAGACAC AGATGAGGAG
    GTCAGAGAGT TCTTGCAAAA TAACCTGCAC CTTCAGGGAA AGGTTGATAA CCCATCTATG
    AGGTGGCAGA TGGCACTGTA TAAGGAAATG GCAGGAAAGG CTGAAGATGC AGATGCCCCA
    GAGAATGTGG TCAAGAGGGT TCAGGAGGTC TCTGCGGTCC TTTACCACAT TGAAGTGACG
    GAGCACCCTT TCAAGTCCAA AAAGATGGTT TGGCACAAAC TGCTGTCGAA GCAGCGGCGT
    AGAGCGGTGG TGGCCTGTTT CAGGATGACT CCACTTTACA ACTTGCCCAG ACACAGAGCA
    TCTAATATGT TCCTGGATGG GTACAAGCGC AACTGGATAA GAAATGAAGG CTATTCCTTT
    GAGGACAGAA TGATTGATGA CCTGTCTAAA GCATTGGAGG AGGAAGAAGA GGAGGAAGAG
    GAAAAGGAGA CAAAGCCAGA CCCTCTTCAT CAGCTCATCC TTCACTTTAG TCGCACAGCA
    CTCACTGAGA AAAGTAAACT GGATATAGAT CACCTATACA TGGCATATGC TGATATCATG
    GCTAAGAGTT GCCACATGGA TGAAGAGGAT GAGGGTGGAG AGGAGGAGAA TGAGGGTGGA
    GAGGAGGAGA TGTCCTTTGA GGTGCGACAG ACAGAGATGG AAAAGGAAAT GGAAAAGCAA
    AGACTGCTCT ACCAGCAGTC GCGTCTACAC AACCGAGGGG CTGCTGAAAT GGTCCTGCAG
    ATGATCAGTG CCTGCAAGGG TGAGACTGGT TGTATGGTCT CTTCCACCCT AAAACTTGGC
    ATTTCTATTT TAAATGGCGG GAACTGTGAA GTTCAGCAGA AAATGCTGGA CTATCTGAAA
    GACAAGAAAG ATGTGGGTTT CTTTCTCAGT GTTCAGGCTT TAATGCAGAC CTGCAGTGTT
    TTGGACCTGA ATGCCTTTGA GAGGCAGAAT AAAGCTGAGG GTCTAGGTAT GGTGTCAGAA
    GAGGGTACAA ACATGAAGCT AGAACGGGGT GAGAAGGTCA TGGCTGATGA CGAGTTCACT
    TGCGACCTCT TCCGCTTTCT CCAGCTCCTC TGTGAGGGTC ACAACAATGA CTTCCAAAAT
    TACCTCAGAA CACAGACAGG CAGCACCACC ACCATCAATG TCATTATCTG TACAGTGGAT
    TACCTGCTTA GACTACAGGA GTCCATCAGT GATTTCTACT GGTATTACTC GGGAAAAGAC
    ATCATAGATG AACCAGGAAA GCGAAACTTC TCTAAAGCTA TGACTGTGGC CAAACAGGTT
    TTCAACAGTC TTACCGAGTA TATCCAGGGT CCCTGCACAG GAAACCAGCA GTCCCTGGCT
    CACAGTCGCT TATGGGATGC TGTGGTGGGC TTTCTGCACG TGTTTGCTCA TATGATGATG
    AAACTGGCTC AGGGAAGAGA TTCCAGTCAG ATTGGTCTTC TGAAAGAGTT GTTGGACCTT
    CAGAAAGACA TGGTGGTCAT GTTATTGTCC TTGTTGGAGG GTAATGTTGT AAATGGCACC
    ATTGCTCGCC AGATGGTGGA CATGCTAGTG GAATCCTCCA GCAATGTAGA AATGATCCTT
    AAGTTCTTCG ACATGTTCCT CAAGCTGAAG GACATTGTTG CGTCTGACGC GTTTCGTGAC
    TACGTGACCG ACCCTAGAGG TTTGATATCC AAGAAAGACT TTCAGAAATC CATGGACAGC
    CAGAAACAGT ACACACCTTC AGAGATCCAA TTCCTATTGT CATGCTCTGA GGCTGATGAG
    AATGAGATGA TCAATTACGA GGAGTTTGCA AGTCGCTTTC AAGAGCCGGC CAAAGATATT
    GGTTTTAACA TAGCGGTGCT GCTCACCAAC CTCTCCGAAC ATGTTCCTCA CGATACAAGA
    CTGCAGAACT TCCTCGAACA AGCCGAGAGC GTCCTGAACT ATTTCCGCCC ATTCTTGGGT
    CGCATCGAGA TCATGGGCGC CAGCAGGAAA ATCGAGAGAA TCTACTTCGA GATCAGCGAG
    GTGAATCGCA AGCAGTGGGA AATGCCTCAA GTCAAAGAAT CGAAGAGACA GTTCATATTC
    GACGTAGTCA ATGAAGGCGG CGAATCGGAG AAGATGGAGC TTTTCGTCAA CTTCTGCGAG
    GACACCATAT TCGAGATGAA CATCGCGTCG CAGATTTCAG AACAAGACGA GGAAGAGAAA
    GAAGAGGATG ATATTGAGGA AGCTGAAGGA GGAGATGCTG GTGATGAAGA GGGGGGTGGC
    GAGGAGGGAC AACCAGAATC AAGCTCTGCA TTTGCCGATT TCATTCAAAG CATCATGAAT
    TTTCTGGGTA TGTTCACCTT CAGGAATATT CGCAGACAGT ACCGCAGACT CCGCAAGATG
    ACCATTAAGG AGATCGTGGT AGGGCTTGCT ACCTTCATGT GGACGATTCT CTTGGGCATC
    CTCCATTTCA TTTACAGTGT TTGTAAAGGC TTCTTCCTCC TCATCTGGCA CACGCTGTTT
    GGAGGAGGTT TGGTTGAAGG GGCCAAAAAC ATGACAGTAA CTGAGTTACT AGCCAGCATG
    CCAGATCCCA CACAGGACGA GGTGCATGGG GACTTACCTG GAGAACCGAG GGGAGGAGAG
    GAACAGGAGA CTGGAGGGAT AATGGACTCG ATGGATGCTG GAGGGGGAGA GGAAGAGGAT
    GAGGATATCC AGGAGAAAGA TGGTGGAAAG ATTCCTGGTA TAGATACACC TGGTGGACTT
    GGAGATATGG GTGATACAAC TCCTGTAGAA CCGCCGACTC CCGAGGGTAC CCCTTTGTTG
    AAGAGGAAAG CGCAACCAGA TGAAGCAGGA CCTGAACAGC CTCCTGCTAT TGAAGAGCCT
    CCTCCTGAGC CTGAGAAAGC AGACATTGAG AATGGTGAGA AGGCTGAGAA GGAAGTGGAG
    GTCAAGCCTG AGCCGATACC AGAAGAACCG AAACCTCAGA GAGCCACCAA AGCCAAGAAG
    GAGAAGAAAT CAGTCAAGGG TGCAGGATTT GAGCTCTGGA ATGAGCTGGA TGTGCAAAGA
    AATAAATTCA TGAACTGCTT ATCTCGTAAC TTCTACAACA TTCGTTTCCT GGCTCTGTTC
    CTTGCCTTTG CCTTGAACTT CATTCTTCTC TTCTACAAGG TATCTGATAC TCCCCCCAGT
    TCCGATGAAT TTGAAGGTTC TGGTATCTTT GAAGGCTCTG GGCTGTTTGA AGGCTCTGCG
    GAGGATTTCG AGGGCTCTGG TGGAGAGGAT GGAGGTGATG ATGAGGATGA AGAAGGACCT
    GTGTATTATT TTCTGGAAGA AAGCACGGGA TACATGCAGC CCACTCTGTC CTTCCTGGCC
    ATTTTGCACA CCGTCATTGC TTTCATATCT ATCATTGGAT ACAACTGCCT CAAGATTCCA
    CTGGTCATCT TTAAGAGAGA GAAAGAGTTG GCGAGAAAAC TGGAGTTTGA TGGTCTTTAC
    ATCACTGAAC AGCCGGCAGA TGATGACATT AAAGGCCAGT GGGACAGACT GGTGTTAAAC
    ACTCCCTCTT TTCCCACCAA TTACTGGGAC AAATTTGTGA AGCGAAAGGT TCTGGATAAA
    TATGGAGATA TTTATGGACG AGAGAGAATC GCAGAGCTGC TGGGTATGGA TCTGGCATCT
    CTGGATGTCA GCAAACAACA GACAGACAAG AAACCAGAAG AGCCAGACAA CTCTATGCTT
    GCATGGTTCA CTTCCATTGA CATCAAGTAT CAGATCTGGA AATTTGGAGT TGTGTTCACA
    GACAATACTT TCCTCTACCT GGTGTGGTAC ACAGTGATGT CCCTTCTTGG ACATTACAAC
    AACTTTTTCT TTGCTTGTCA CCTGCTGGAC ATCGCAATGG GTGTCAAGAC ACTGCGTACA
    ATTCTTTCCT CCGTCACACA CAATGGAAAA CAGCTGATGA TGACTGTGGG TTTGCTGGCT
    GTGGTTGTGT ACCTCTACAC TGTGGTGGCC TTCAACTTCT TCAGGAAGTT CTACAATAAG
    AGCGAGGATG AGGATGAGCC GGACATGAAG TGTGACGACA TGATGACGTG TTATTTGTTC
    CACATGTATG TGGGTGTACG GGCAGGTGGA GGTATAGGAG ATGAGATCGA GGACCCTGCT
    GGAGATGAGT ATGAGCTCTA TCGGGTGGTC TTCGACATCA CCTTCTTCTT CTTTGTCATC
    GTCATCCTGC TGGCCATCAT TCAGGGTCTG ATCATTGATG CCTTTGGTGA ACTGAGAGAC
    CAGCAGGAGC AAGTGAAAGA GGACATGGAG ACCAAATGCT TTATATGTGG CATTGGGAGC
    GACTACTTTG ATACAACGCC CCATGGCTTT GAGACCCATA CACTTGAGGA ACACAACCTG
    GCCAATTACA TGTTTTTCCT GATGTACTTA ATCAACAAGG ATGAAACAGA GCACACTGGC
    CAGGAATCAT ACGTGTGGAA GATGTACCAG GAGAGATGCT GGGATTTCTT CCCTGCCGGA
    GACTGCTTCA GAAAGCAATA TGAAGACCAG CTTGGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021327992.1
    CDS188..8986
    Translation

    Target ORF information:

    RefSeq Version XM_021472317.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio ryanodine receptor 1a (skeletal) (ryr1a), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021472317.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    ATGATCTACT GGGCTCAAGA GTCTTTCATC CAGAACCCTG AGCTGGTGCG CCTCATGTTT 
    AGTCTGCTAC ACCGGCAGTA TGACGCTTTG GGTGAGCTGA TTCGAGCTTT GCCCAAAGCT
    TATATCATTA ATGCTGTTTC TATACAAGAC ACAATGGAGC TCCTGGAGTG TCTGGGACAG
    ATACGCTCCT TGCTCATCGT TCAGATGGGA CCAGAGGAAG AGAGGCTGAT GATCCAGAGT
    ATCGGAAATA TTATGAGTAA CAGGGTATTT TACCAGCACC CCAACTTGAT GAGAGCTCTG
    GGAATGCATG AGACAGTCAT GGAGGTTATG GTGAACGTAC TCGGAGGCGG AGATTCCAAG
    GAGATCAGAT TCCCTCGAAT GGTCACCAAC TGCTGTCGTT TCCTGTGTTA TTTCTGTCGT
    ATCAGCAGAC AGAATCAGCG CTCGATGTTT GATCATCTGG GCTACCTCCT GCAGAACAGT
    GGCATTGGGC TGGGAATGCG TGGCTCCACT CCTCTTGATG TTGCTGCTGC TTCCTGTATC
    GATAATAATG AGCTTGCCCT GGCTCTCCAA GAGCAAGACC TGGAGATGGT AGTAAAGTAC
    TTGGCAGGCT GTGGTCTGCA GAGCTGCCCT ATGCTCTTGT CAAAGGGTTA CCCGGATATT
    GGATGGAACC CAGTTGGAGG AGAAAAGTAT CTGGATTTCC TGCGCTTTGC TGTGTTTGTG
    AATGGAGAGA GTGTGGAGGA GAACGCTAAT GTGGTGGTCC GGCTGCTGAT CAGGAGGCCT
    GAGTGCTTTG GTCCTGCACT GAGAGGAGAA GGGGGCAATG GTCTGCTGGC TGCCATTGAA
    GAAGCCATCA AGATCTCAGA GGACCCTGCA AGAGATGGAC CCACTGTTAA AAAAGACAGA
    CGCTTCCCGA TGTTTGGAGG TGAAGAGCAG CATGAGGAGA ACAGAGTGCA CCTAGGAAAT
    GCCATCATGT CATTTTACTC AGCTCTTATT GATCTGCTGG GTCGCTGTGC TCCTGAGATG
    CATTTGATCC AGGCTGGTAA AGGTGAAGCT TTGAGAATCA GGGCTATTCT TCGGTCGCTT
    GTGCCCATTG AGGATCTGGT CGGTGTGATC AGCTTGTCAG TCCAGATTCC TGCTTTTGGA
    AAAGATAACA GCATTATTGA GCCGAAGATG TCTGCTAGTT TTGTTCCTGA TCATAAAGCA
    CCAATGGTCC TGTTCCTGGA CAGAGTTTAT GGCATCGACA ATCAGGACTT CCTATTGCAT
    GTACTAGAAG TGGGTTTCCT GCCTGATATG AGGGCGGCAG CCTCCTTAGA CACAGCTGCA
    TTCAGCACTA CAGAAATGGC CTTGGCTCTG AACCGTTACC TGTGCTCCGC CGTGCTTCCT
    CTCATCACTA AATGTGCTCC TTTGTTCGCC GGGACGGACC ACCGAGCCAT CATGATCGAC
    TCCATGCTTC ACACCATCTA CAGGCTGTCT CGAGGACGCT CGCTAACTAA AGCACAAAGA
    GATGTTATTG AGGAATGCCT CATGTCTTTG TGCAGGTATC TGCGCCCTTC CATGCTCCAG
    CACCTTCTCA GGCGGCTAGT ATTTGATGTG CCTATTCTGA ATGAGTATGC CAAAATGCCC
    CTTAAGCTGC TTACCAATCA TTATGAGCGT TGTTGGAAAT ATTATTGCTT GCCTAATGGC
    TGGGCTAACT TTGGCGTATC ATCAGAAGAG GAGCTTCATC TGACCCGTAA ACTCTTTTGG
    GGGATCTTTG AATCTCTGGC TCACAAGAAA TTCGATGCAG AGCTGTTCAA AATCGCCATG
    CCTTGTATAT GTGCGATCGC TGGTGCAATT CCTCCTGATT ATGTGGACGC CAGCTACTCC
    TCCAAAACCG AAAAAAAGGC TTCTGTTGAT GCTGAAGGGA ACTTTGACCC CAAACCTGTT
    GATACCACTA ACACTATAAT TCCCGAGAAG TTGGACGGGT TCATCAACAG ATATGCAGAA
    TATACGCACG ACAGATGGGC TTTTGAAAAA ATTCAAAACA ACTGGACATT TGGTGAGGTG
    ATGGATGAAA ATGCCAAGAC TCATCCCATG CTCAGACCTT ACAAAACATT CTCTGAAAAG
    GATAAAGAAA TCTACCGCTG GCCTATTAAA GAGTCCATCA AGGCCATGAT TGCCTGGGAG
    TGGACACTGG ACAAAGCCAG AGATGGAGAG GATGAGAAAG TTGAGAAGAA AACAGCCCGT
    AAGATCTCAC AGACTGCACA GGCAACCTAT GACCCCAGTC ATGGCTATAG CCCCCAACCT
    ATTGATATCT CAGGCATGAC ACTGTCCAGA GAGCTACAGT CCATGGCAGA GCAGCTCGCA
    GAAAACTACC ACAACACCTG GGGCCGTAAG AAGAAAATGG AATTACAAGC AAAAGGAGGA
    GGTACACACC CTTTGCTTGT GCCTTATGAC ACACTGACGG CTAAAGAAAA GGCACGAGAT
    AGAGAGAAAG CGTATGAGCT ACTCAAGTTT CTGCAGCTCA ATGGATATGC TGTGACAAGA
    GGGCTCAAAG ACATGGAAAG TGACATTTCA TCTATCGAGA AGCGTTTTGC GTATGGCTTC
    CTACAGAAGC TTCTGAAGTG GATGGACATT GCTCAGGAGT TCATAGCACA TCTTGAGGCG
    GTTGTGAGCA GCGGTCGTGT GGAAAAGTCA CCACATGAGC AAGAAATCAA GTTCTTTGCC
    AAGATTCTTT TGCCTTTGAT AAATCAGTAT TTTAAAAACC ACTGCCTTTA CTTCCTCTCC
    ACACCGGCCA AAATCCTAGG CAGCGGCGGA CACGCCTCAA ACAAGGAAAA GGAGATGATT
    GCAAGCATTT TCTGTAAGAT GTCTGCCCTG GTGCGACACA GAGTGTTTCT CTTTGGCACG
    GATGCGCCTG CTATTGTCAA CTGCCTTCAT ATCCTCGCAC GTTCGCTTGA TGCCAGGACG
    GTGATGAAAT CCGGGCCTGA GATTGTGAAA GCAGGACTGC GTTTGTTCTT TGAGAGTGCA
    GCTGATGACA TTGAAAAGAT GGTGGAGAAT CTCAAACTTG GCAAAGTGTC CAAAGGCAAT
    CAGCCAGTGA AAGGTGTGTC CCAGAATATA AACTACACCA CAACGGCTTT GCTTCCTGTC
    CTCACATCAC TGTTTGACCA CATCGCTCAA CACCAATTCG GAGATGATGT TATCCTGGAT
    GATTTGCAGA TGTCCTGCTA CCGAATCATG TGTAGCATCT ACTCTCTTGG GACTGTAAAA
    ACTCCACATG CAGAGAGGCA TAGACCAGCC CTTGGAGAAT GTCTCGCTCA TCTCGCTGCA
    GCAATGCCTG TTGCCTACCT GGAGCCGCAT CTTAATGAAT ACAATGCCTT TTCAGTCTAC
    ACTACCAAGA CACCGAGAGA AAGAGCTATC CTTGGCCTTC CAAACCAAGT TCAAGAGCTG
    TGTTTGGACA TTCCTGAGCT TGACGTTTTA CTTAAGGAGA TCGGAGACCT GGCTGAATCG
    GGAGCTCGAT ACACTGAAAT GCCACATGTG ATTGAGGTCA CTCTGCCCAT GCTGTGTAAC
    TATCTGCCCC GCTGGTGGGA GAGAGGACTG GAGAACTTCC CGGATCTGGA GGGTAAGCTG
    TGCACCGACG TCACCTCTGA ATCCCTCAAC CAGCTTTTGG GAAGCATCAT GAAAATCGTG
    GTCAACAACT TGGGAATCGA CGAAGCCTCC TGGATGAAGA GACTTGCAGT GTTCTCCCAA
    CCCATTGTGA GCCGAGCCAA ACCAGAGATG CTCAAATCCC ACTTCATCCC CACCATGGAG
    AAGCTGAAGA AGCGAACGTC TAAAGTTGTA GCCGAGGAGG AGCATCTGCG TTTGGAGGGA
    AAGTCTGAGG GAGATGAGGA AGAGGGAACC ATCAGAGACG AGTTTGCAGT TCTCTGTAGA
    GACCTCTATG CTCTATATCC TCTTCTCATC CGCTATGTGG ATAACAACAG AGCAAGATGG
    CTGACGTTTC GAGACCCAGA TGCTGAGGAG TTGTTCCGCA TGGTGGGAGA GGTCTTCATC
    TTCTGGTCAA AATCTCACAA TTTCAAGCGA GAGGAGCAAA ACTTTGTGGT GATGAACGAA
    ATCAACAATA TGTCCTTTCT CACTGCTGAC AGTAAGAGCA AGATGAGCAA GGCTGGAGGG
    TCGGATGTGG AGCGTACCAA GAAAAAGAGA CGGGGGGATC GATACTCTGT GCAAACGTCC
    CTCATTGTGG CCGCACTGAA GAAAATGCTT CCCATTGGAC TTAACATGTG TTCTCCTGCA
    GACCAGGAGC TGATTAATCT GGCAAAGACA CGTTATTCTC TGAAAGACAC AGATGAGGAG
    GTCAGAGAGT TCTTGCAAAA TAACCTGCAC CTTCAGGGAA AGGTTGATAA CCCATCTATG
    AGGTGGCAGA TGGCACTGTA TAAGGAAATG GCAGGAAAGG CTGAAGATGC AGATGCCCCA
    GAGAATGTGG TCAAGAGGGT TCAGGAGGTC TCTGCGGTCC TTTACCACAT TGAAGTGACG
    GAGCACCCTT TCAAGTCCAA AAAGATGGTT TGGCACAAAC TGCTGTCGAA GCAGCGGCGT
    AGAGCGGTGG TGGCCTGTTT CAGGATGACT CCACTTTACA ACTTGCCCAG ACACAGAGCA
    TCTAATATGT TCCTGGATGG GTACAAGCGC AACTGGATAA GAAATGAAGG CTATTCCTTT
    GAGGACAGAA TGATTGATGA CCTGTCTAAA GCATTGGAGG AGGAAGAAGA GGAGGAAGAG
    GAAAAGGAGA CAAAGCCAGA CCCTCTTCAT CAGCTCATCC TTCACTTTAG TCGCACAGCA
    CTCACTGAGA AAAGTAAACT GGATATAGAT CACCTATACA TGGCATATGC TGATATCATG
    GCTAAGAGTT GCCACATGGA TGAAGAGGAT GAGGGTGGAG AGGAGGAGAA TGAGGGTGGA
    GAGGAGGAGA TGTCCTTTGA GGTGCGACAG ACAGAGATGG AAAAGGAAAT GGAAAAGCAA
    AGACTGCTCT ACCAGCAGTC GCGTCTACAC AACCGAGGGG CTGCTGAAAT GGTCCTGCAG
    ATGATCAGTG CCTGCAAGGG TGAGACTGGT TGTATGGTCT CTTCCACCCT AAAACTTGGC
    ATTTCTATTT TAAATGGCGG GAACTGTGAA GTTCAGCAGA AAATGCTGGA CTATCTGAAA
    GACAAGAAAG ATGTGGGTTT CTTTCTCAGT GTTCAGGCTT TAATGCAGAC CTGCAGTGTT
    TTGGACCTGA ATGCCTTTGA GAGGCAGAAT AAAGCTGAGG GTCTAGGTAT GGTGTCAGAA
    GAGGGTACAA ACATGAAGCT AGAACGGGGT GAGAAGGTCA TGGCTGATGA CGAGTTCACT
    TGCGACCTCT TCCGCTTTCT CCAGCTCCTC TGTGAGGGTC ACAACAATGA CTTCCAAAAT
    TACCTCAGAA CACAGACAGG CAGCACCACC ACCATCAATG TCATTATCTG TACAGTGGAT
    TACCTGCTTA GACTACAGGA GTCCATCAGT GATTTCTACT GGTATTACTC GGGAAAAGAC
    ATCATAGATG AACCAGGAAA GCGAAACTTC TCTAAAGCTA TGACTGTGGC CAAACAGGTT
    TTCAACAGTC TTACCGAGTA TATCCAGGGT CCCTGCACAG GAAACCAGCA GTCCCTGGCT
    CACAGTCGCT TATGGGATGC TGTGGTGGGC TTTCTGCACG TGTTTGCTCA TATGATGATG
    AAACTGGCTC AGGGAAGAGA TTCCAGTCAG ATTGGTCTTC TGAAAGAGTT GTTGGACCTT
    CAGAAAGACA TGGTGGTCAT GTTATTGTCC TTGTTGGAGG GTAATGTTGT AAATGGCACC
    ATTGCTCGCC AGATGGTGGA CATGCTAGTG GAATCCTCCA GCAATGTAGA AATGATCCTT
    AAGTTCTTCG ACATGTTCCT CAAGCTGAAG GACATTGTTG CGTCTGACGC GTTTCGTGAC
    TACGTGACCG ACCCTAGAGG TTTGATATCC AAGAAAGACT TTCAGAAATC CATGGACAGC
    CAGAAACAGT ACACACCTTC AGAGATCCAA TTCCTATTGT CATGCTCTGA GGCTGATGAG
    AATGAGATGA TCAATTACGA GGAGTTTGCA AGTCGCTTTC AAGAGCCGGC CAAAGATATT
    GGTTTTAACA TAGCGGTGCT GCTCACCAAC CTCTCCGAAC ATGTTCCTCA CGATACAAGA
    CTGCAGAACT TCCTCGAACA AGCCGAGAGC GTCCTGAACT ATTTCCGCCC ATTCTTGGGT
    CGCATCGAGA TCATGGGCGC CAGCAGGAAA ATCGAGAGAA TCTACTTCGA GATCAGCGAG
    GTGAATCGCA AGCAGTGGGA AATGCCTCAA GTCAAAGAAT CGAAGAGACA GTTCATATTC
    GACGTAGTCA ATGAAGGCGG CGAATCGGAG AAGATGGAGC TTTTCGTCAA CTTCTGCGAG
    GACACCATAT TCGAGATGAA CATCGCGTCG CAGATTTCAG AACAAGACGA GGAAGAGAAA
    GAAGAGGATG ATATTGAGGA AGCTGAAGGA GGAGATGCTG GTGATGAAGA GGGGGGTGGC
    GAGGAGGGAC AACCAGAATC AAGCTCTGCA TTTGCCGATT TCATTCAAAG CATCATGAAT
    TTTCTGGGTA TGTTCACCTT CAGGAATATT CGCAGACAGT ACCGCAGACT CCGCAAGATG
    ACCATTAAGG AGATCGTGGT AGGGCTTGCT ACCTTCATGT GGACGATTCT CTTGGGCATC
    CTCCATTTCA TTTACAGTGT TTGTAAAGGC TTCTTCCTCC TCATCTGGCA CACGCTGTTT
    GGAGGAGGTT TGGTTGAAGG GGCCAAAAAC ATGACAGTAA CTGAGTTACT AGCCAGCATG
    CCAGATCCCA CACAGGACGA GGTGCATGGG GACTTACCTG GAGAACCGAG GGGAGGAGAG
    GAACAGGAGA CTGGAGGGAT AATGGACTCG ATGGATGCTG GAGGGGGAGA GGAAGAGGAT
    GAGGATATCC AGGAGAAAGA TGGTGGAAAG ATTCCTGGTA TAGATACACC TGGTGGACTT
    GGAGATATGG GTGATACAAC TCCTGTAGAA CCGCCGACTC CCGAGGGTAC CCCTTTGTTG
    AAGAGGAAAG CGCAACCAGA TGAAGCAGGA CCTGAACAGC CTCCTGCTAT TGAAGAGCCT
    CCTCCTGAGC CTGAGAAAGC AGACATTGAG AATGGTGAGA AGGCTGAGAA GGAAGTGGAG
    GTCAAGCCTG AGCCGATACC AGAAGAACCG AAACCTCAGA GAGCCACCAA AGCCAAGAAG
    GAGAAGAAAT CAGTCAAGGG TGCAGGATTT GAGCTCTGGA ATGAGCTGGA TGTGCAAAGA
    AATAAATTCA TGAACTGCTT ATCTCGTAAC TTCTACAACA TTCGTTTCCT GGCTCTGTTC
    CTTGCCTTTG CCTTGAACTT CATTCTTCTC TTCTACAAGG TATCTGATAC TCCCCCCAGT
    TCCGATGAAT TTGAAGGTTC TGGTATCTTT GAAGGCTCTG GGCTGTTTGA AGGCTCTGCG
    GAGGATTTCG AGGGCTCTGG TGGAGAGGAT GGAGGTGATG ATGAGGATGA AGAAGGACCT
    GTGTATTATT TTCTGGAAGA AAGCACGGGA TACATGCAGC CCACTCTGTC CTTCCTGGCC
    ATTTTGCACA CCGTCATTGC TTTCATATCT ATCATTGGAT ACAACTGCCT CAAGATTCCA
    CTGGTCATCT TTAAGAGAGA GAAAGAGTTG GCGAGAAAAC TGGAGTTTGA TGGTCTTTAC
    ATCACTGAAC AGCCGGCAGA TGATGACATT AAAGGCCAGT GGGACAGACT GGTGTTAAAC
    ACTCCCTCTT TTCCCACCAA TTACTGGGAC AAATTTGTGA AGCGAAAGGT TCTGGATAAA
    TATGGAGATA TTTATGGACG AGAGAGAATC GCAGAGCTGC TGGGTATGGA TCTGGCATCT
    CTGGATGTCA GCAAACAACA GACAGACAAG AAACCAGAAG AGCCAGACAA CTCTATGCTT
    GCATGGTTCA CTTCCATTGA CATCAAGTAT CAGATCTGGA AATTTGGAGT TGTGTTCACA
    GACAATACTT TCCTCTACCT GGTGTGGTAC ACAGTGATGT CCCTTCTTGG ACATTACAAC
    AACTTTTTCT TTGCTTGTCA CCTGCTGGAC ATCGCAATGG GTGTCAAGAC ACTGCGTACA
    ATTCTTTCCT CCGTCACACA CAATGGAAAA CAGCTGATGA TGACTGTGGG TTTGCTGGCT
    GTGGTTGTGT ACCTCTACAC TGTGGTGGCC TTCAACTTCT TCAGGAAGTT CTACAATAAG
    AGCGAGGATG AGGATGAGCC GGACATGAAG TGTGACGACA TGATGACGTG TTATTTGTTC
    CACATGTATG TGGGTGTACG GGCAGGTGGA GGTATAGGAG ATGAGATCGA GGACCCTGCT
    GGAGATGAGT ATGAGCTCTA TCGGGTGGTC TTCGACATCA CCTTCTTCTT CTTTGTCATC
    GTCATCCTGC TGGCCATCAT TCAGGGTCTG ATCATTGATG CCTTTGGTGA ACTGAGAGAC
    CAGCAGGAGC AAGTGAAAGA GGACATGGAG ACCAAATGCT TTATATGTGG CATTGGGAGC
    GACTACTTTG ATACAACGCC CCATGGCTTT GAGACCCATA CACTTGAGGA ACACAACCTG
    GCCAATTACA TGTTTTTCCT GATGTACTTA ATCAACAAGG ATGAAACAGA GCACACTGGC
    CAGGAATCAT ACGTGTGGAA GATGTACCAG GAGAGATGCT GGGATTTCTT CCCTGCCGGA
    GACTGCTTCA GAAAGCAATA TGAAGACCAG CTTGGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa145155
    Clone ID Related Accession (Same CDS sequence) XM_021472318.1
    Accession Version XM_021472318.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8781bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product ryanodine receptor 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394792.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    ATGATCTACT GGGCTCAAGA GTCTTTCATC CAGAACCCTG AGCTGGTGCG CCTCATGTTT 
    AGTCTGCTAC ACCGGCAGTA TGACGCTTTG GGTGAGCTGA TTCGAGCTTT GCCCAAAGCT
    TATATCATTA ATGCTGTTTC TATACAAGAC ACAATGGAGC TCCTGGAGTG TCTGGGACAG
    ATACGCTCCT TGCTCATCGT TCAGATGGGA CCAGAGGAAG AGAGGCTGAT GATCCAGAGT
    ATCGGAAATA TTATGAGTAA CAGGGTATTT TACCAGCACC CCAACTTGAT GAGAGCTCTG
    GGAATGCATG AGACAGTCAT GGAGGTTATG GTGAACGTAC TCGGAGGCGG AGATTCCAAG
    GAGATCAGAT TCCCTCGAAT GGTCACCAAC TGCTGTCGTT TCCTGTGTTA TTTCTGTCGT
    ATCAGCAGAC AGAATCAGCG CTCGATGTTT GATCATCTGG GCTACCTCCT GCAGAACAGT
    GGCATTGGGC TGGGAATGCG TGGCTCCACT CCTCTTGATG TTGCTGCTGC TTCCTGTATC
    GATAATAATG AGCTTGCCCT GGCTCTCCAA GAGCAAGACC TGGAGATGGT AGTAAAGTAC
    TTGGCAGGCT GTGGTCTGCA GAGCTGCCCT ATGCTCTTGT CAAAGGGTTA CCCGGATATT
    GGATGGAACC CAGTTGGAGG AGAAAAGTAT CTGGATTTCC TGCGCTTTGC TGTGTTTGTG
    AATGGAGAGA GTGTGGAGGA GAACGCTAAT GTGGTGGTCC GGCTGCTGAT CAGGAGGCCT
    GAGTGCTTTG GTCCTGCACT GAGAGGAGAA GGGGGCAATG GTCTGCTGGC TGCCATTGAA
    GAAGCCATCA AGATCTCAGA GGACCCTGCA AGAGATGGAC CCACTGTTAA AAAAGACAGA
    CGCTTCCCGA TGTTTGGAGG TGAAGAGCAG CATGAGGAGA ACAGAGTGCA CCTAGGAAAT
    GCCATCATGT CATTTTACTC AGCTCTTATT GATCTGCTGG GTCGCTGTGC TCCTGAGATG
    CATTTGATCC AGGCTGGTAA AGGTGAAGCT TTGAGAATCA GGGCTATTCT TCGGTCGCTT
    GTGCCCATTG AGGATCTGGT CGGTGTGATC AGCTTGTCAG TCCAGATTCC TGCTTTTGGA
    AAAGATAACA GCATTATTGA GCCGAAGATG TCTGCTAGTT TTGTTCCTGA TCATAAAGCA
    CCAATGGTCC TGTTCCTGGA CAGAGTTTAT GGCATCGACA ATCAGGACTT CCTATTGCAT
    GTACTAGAAG TGGGTTTCCT GCCTGATATG AGGGCGGCAG CCTCCTTAGA CACAGCTGCA
    TTCAGCACTA CAGAAATGGC CTTGGCTCTG AACCGTTACC TGTGCTCCGC CGTGCTTCCT
    CTCATCACTA AATGTGCTCC TTTGTTCGCC GGGACGGACC ACCGAGCCAT CATGATCGAC
    TCCATGCTTC ACACCATCTA CAGGCTGTCT CGAGGACGCT CGCTAACTAA AGCACAAAGA
    GATGTTATTG AGGAATGCCT CATGTCTTTG TGCAGGTATC TGCGCCCTTC CATGCTCCAG
    CACCTTCTCA GGCGGCTAGT ATTTGATGTG CCTATTCTGA ATGAGTATGC CAAAATGCCC
    CTTAAGCTGC TTACCAATCA TTATGAGCGT TGTTGGAAAT ATTATTGCTT GCCTAATGGC
    TGGGCTAACT TTGGCGTATC ATCAGAAGAG GAGCTTCATC TGACCCGTAA ACTCTTTTGG
    GGGATCTTTG AATCTCTGGC TCACAAGAAA TTCGATGCAG AGCTGTTCAA AATCGCCATG
    CCTTGTATAT GTGCGATCGC TGGTGCAATT CCTCCTGATT ATGTGGACGC CAGCTACTCC
    TCCAAAACCG AAAAAAAGGC TTCTGTTGAT GCTGAAGGGA ACTTTGACCC CAAACCTGTT
    GATACCACTA ACACTATAAT TCCCGAGAAG TTGGACGGGT TCATCAACAG ATATGCAGAA
    TATACGCACG ACAGATGGGC TTTTGAAAAA ATTCAAAACA ACTGGACATT TGGTGAGGTG
    ATGGATGAAA ATGCCAAGAC TCATCCCATG CTCAGACCTT ACAAAACATT CTCTGAAAAG
    GATAAAGAAA TCTACCGCTG GCCTATTAAA GAGTCCATCA AGGCCATGAT TGCCTGGGAG
    TGGACACTGG ACAAAGCCAG AGATGGAGAG GATGAGAAAG TTGAGAAGAA AACAGCCCGT
    AAGATCTCAC AGACTGCACA GGCAACCTAT GACCCCAGTC ATGGCTATAG CCCCCAACCT
    ATTGATATCT CAGGCATGAC ACTGTCCAGA GAGCTACAGT CCATGGCAGA GCAGCTCGCA
    GAAAACTACC ACAACACCTG GGGCCGTAAG AAGAAAATGG AATTACAAGC AAAAGGAGGA
    GGTACACACC CTTTGCTTGT GCCTTATGAC ACACTGACGG CTAAAGAAAA GGCACGAGAT
    AGAGAGAAAG CGTATGAGCT ACTCAAGTTT CTGCAGCTCA ATGGATATGC TGTGACAAGA
    GGGCTCAAAG ACATGGAAAG TGACATTTCA TCTATCGAGA AGCGTTTTGC GTATGGCTTC
    CTACAGAAGC TTCTGAAGTG GATGGACATT GCTCAGGAGT TCATAGCACA TCTTGAGGCG
    GTTGTGAGCA GCGGTCGTGT GGAAAAGTCA CCACATGAGC AAGAAATCAA GTTCTTTGCC
    AAGATTCTTT TGCCTTTGAT AAATCAGTAT TTTAAAAACC ACTGCCTTTA CTTCCTCTCC
    ACACCGGCCA AAATCCTAGG CAGCGGCGGA CACGCCTCAA ACAAGGAAAA GGAGATGATT
    GCAAGCATTT TCTGTAAGAT GTCTGCCCTG GTGCGACACA GAGTGTTTCT CTTTGGCACG
    GATGCGCCTG CTATTGTCAA CTGCCTTCAT ATCCTCGCAC GTTCGCTTGA TGCCAGGACG
    GTGATGAAAT CCGGGCCTGA GATTGTGAAA GCAGGACTGC GTTTGTTCTT TGAGAGTGCA
    GCTGATGACA TTGAAAAGAT GGTGGAGAAT CTCAAACTTG GCAAAGTGTC CAAAGGCAAT
    CAGCCAGTGA AAGGTGTGTC CCAGAATATA AACTACACCA CAACGGCTTT GCTTCCTGTC
    CTCACATCAC TGTTTGACCA CATCGCTCAA CACCAATTCG GAGATGATGT TATCCTGGAT
    GATTTGCAGA TGTCCTGCTA CCGAATCATG TGTAGCATCT ACTCTCTTGG GACTGTAAAA
    ACTCCACATG CAGAGAGGCA TAGACCAGCC CTTGGAGAAT GTCTCGCTCA TCTCGCTGCA
    GCAATGCCTG TTGCCTACCT GGAGCCGCAT CTTAATGAAT ACAATGCCTT TTCAGTCTAC
    ACTACCAAGA CACCGAGAGA AAGAGCTATC CTTGGCCTTC CAAACCAAGT TCAAGAGCTG
    TGTTTGGACA TTCCTGAGCT TGACGTTTTA CTTAAGGAGA TCGGAGACCT GGCTGAATCG
    GGAGCTCGAT ACACTGAAAT GCCACATGTG ATTGAGGTCA CTCTGCCCAT GCTGTGTAAC
    TATCTGCCCC GCTGGTGGGA GAGAGGACTG GAGAACTTCC CGGATCTGGA GGGTAAGCTG
    TGCACCGACG TCACCTCTGA ATCCCTCAAC CAGCTTTTGG GAAGCATCAT GAAAATCGTG
    GTCAACAACT TGGGAATCGA CGAAGCCTCC TGGATGAAGA GACTTGCAGT GTTCTCCCAA
    CCCATTGTGA GCCGAGCCAA ACCAGAGATG CTCAAATCCC ACTTCATCCC CACCATGGAG
    AAGCTGAAGA AGCGAACGTC TAAAGTTGTA GCCGAGGAGG AGCATCTGCG TTTGGAGGGA
    AAGTCTGAGG GAGATGAGGA AGAGGGAACC ATCAGAGACG AGTTTGCAGT TCTCTGTAGA
    GACCTCTATG CTCTATATCC TCTTCTCATC CGCTATGTGG ATAACAACAG AGCAAGATGG
    CTGACGTTTC GAGACCCAGA TGCTGAGGAG TTGTTCCGCA TGGTGGGAGA GGTCTTCATC
    TTCTGGTCAA AATCTCACAA TTTCAAGCGA GAGGAGCAAA ACTTTGTGGT GATGAACGAA
    ATCAACAATA TGTCCTTTCT CACTGCTGAC AGTAAGAGCA AGATGAGCAA GGCTGGAGGG
    TCGGATGTGG AGCGTACCAA GAAAAAGAGA CGGGGGGATC GATACTCTGT GCAAACGTCC
    CTCATTGTGG CCGCACTGAA GAAAATGCTT CCCATTGGAC TTAACATGTG TTCTCCTGCA
    GACCAGGAGC TGATTAATCT GGCAAAGACA CGTTATTCTC TGAAAGACAC AGATGAGGAG
    GTCAGAGAGT TCTTGCAAAA TAACCTGCAC CTTCAGGGAA AGGTTGATAA CCCATCTATG
    AGGTGGCAGA TGGCACTGTA TAAGGAAATG GCAGGAAAGG CTGAAGATGC AGATGCCCCA
    GAGAATGTGG TCAAGAGGGT TCAGGAGGTC TCTGCGGTCC TTTACCACAT TGAAGTGACG
    GAGCACCCTT TCAAGTCCAA AAAGATGGTT TGGCACAAAC TGCTGTCGAA GCAGCGGCGT
    AGAGCGGTGG TGGCCTGTTT CAGGATGACT CCACTTTACA ACTTGCCCAG ACACAGAGCA
    TCTAATATGT TCCTGGATGG GTACAAGCGC AACTGGATAA GAAATGAAGG CTATTCCTTT
    GAGGACAGAA TGATTGATGA CCTGTCTAAA GCATTGGAGG AGGAAGAAGA GGAGGAAGAG
    GAAAAGGAGA CAAAGCCAGA CCCTCTTCAT CAGCTCATCC TTCACTTTAG TCGCACAGCA
    CTCACTGAGA AAAGTAAACT GGATATAGAT CACCTATACA TGGCATATGC TGATATCATG
    GCTAAGAGTT GCCACATGGA TGAAGAGGAT GAGGGTGGAG AGGAGGAGAA TGAGGGTGGA
    GAGGAGGAGA TGTCCTTTGA GGAAAAGGAA ATGGAAAAGC AAAGACTGCT CTACCAGCAG
    TCGCGTCTAC ACAACCGAGG GGCTGCTGAA ATGGTCCTGC AGATGATCAG TGCCTGCAAG
    GGTGAGACTG GTTGTATGGT CTCTTCCACC CTAAAACTTG GCATTTCTAT TTTAAATGGC
    GGGAACTGTG AAGTTCAGCA GAAAATGCTG GACTATCTGA AAGACAAGAA AGATGTGGGT
    TTCTTTCTCA GTGTTCAGGC TTTAATGCAG ACCTGCAGTG TTTTGGACCT GAATGCCTTT
    GAGAGGCAGA ATAAAGCTGA GGGTCTAGGT ATGGTGTCAG AAGAGGGTAC AAACATGAAG
    CTAGAACGGG GTGAGAAGGT CATGGCTGAT GACGAGTTCA CTTGCGACCT CTTCCGCTTT
    CTCCAGCTCC TCTGTGAGGG TCACAACAAT GACTTCCAAA ATTACCTCAG AACACAGACA
    GGCAGCACCA CCACCATCAA TGTCATTATC TGTACAGTGG ATTACCTGCT TAGACTACAG
    GAGTCCATCA GTGATTTCTA CTGGTATTAC TCGGGAAAAG ACATCATAGA TGAACCAGGA
    AAGCGAAACT TCTCTAAAGC TATGACTGTG GCCAAACAGG TTTTCAACAG TCTTACCGAG
    TATATCCAGG GTCCCTGCAC AGGAAACCAG CAGTCCCTGG CTCACAGTCG CTTATGGGAT
    GCTGTGGTGG GCTTTCTGCA CGTGTTTGCT CATATGATGA TGAAACTGGC TCAGGGAAGA
    GATTCCAGTC AGATTGGTCT TCTGAAAGAG TTGTTGGACC TTCAGAAAGA CATGGTGGTC
    ATGTTATTGT CCTTGTTGGA GGGTAATGTT GTAAATGGCA CCATTGCTCG CCAGATGGTG
    GACATGCTAG TGGAATCCTC CAGCAATGTA GAAATGATCC TTAAGTTCTT CGACATGTTC
    CTCAAGCTGA AGGACATTGT TGCGTCTGAC GCGTTTCGTG ACTACGTGAC CGACCCTAGA
    GGTTTGATAT CCAAGAAAGA CTTTCAGAAA TCCATGGACA GCCAGAAACA GTACACACCT
    TCAGAGATCC AATTCCTATT GTCATGCTCT GAGGCTGATG AGAATGAGAT GATCAATTAC
    GAGGAGTTTG CAAGTCGCTT TCAAGAGCCG GCCAAAGATA TTGGTTTTAA CATAGCGGTG
    CTGCTCACCA ACCTCTCCGA ACATGTTCCT CACGATACAA GACTGCAGAA CTTCCTCGAA
    CAAGCCGAGA GCGTCCTGAA CTATTTCCGC CCATTCTTGG GTCGCATCGA GATCATGGGC
    GCCAGCAGGA AAATCGAGAG AATCTACTTC GAGATCAGCG AGGTGAATCG CAAGCAGTGG
    GAAATGCCTC AAGTCAAAGA ATCGAAGAGA CAGTTCATAT TCGACGTAGT CAATGAAGGC
    GGCGAATCGG AGAAGATGGA GCTTTTCGTC AACTTCTGCG AGGACACCAT ATTCGAGATG
    AACATCGCGT CGCAGATTTC AGAACAAGAC GAGGAAGAGA AAGAAGAGGA TGATATTGAG
    GAAGCTGAAG GAGGAGATGC TGGTGATGAA GAGGGGGGTG GCGAGGAGGG ACAACCAGAA
    TCAAGCTCTG CATTTGCCGA TTTCATTCAA AGCATCATGA ATTTTCTGGG TATGTTCACC
    TTCAGGAATA TTCGCAGACA GTACCGCAGA CTCCGCAAGA TGACCATTAA GGAGATCGTG
    GTAGGGCTTG CTACCTTCAT GTGGACGATT CTCTTGGGCA TCCTCCATTT CATTTACAGT
    GTTTGTAAAG GCTTCTTCCT CCTCATCTGG CACACGCTGT TTGGAGGAGG TTTGGTTGAA
    GGGGCCAAAA ACATGACAGT AACTGAGTTA CTAGCCAGCA TGCCAGATCC CACACAGGAC
    GAGGTGCATG GGGACTTACC TGGAGAACCG AGGGGAGGAG AGGAACAGGA GACTGGAGGG
    ATAATGGACT CGATGGATGC TGGAGGGGGA GAGGAAGAGG ATGAGGATAT CCAGGAGAAA
    GATGGTGGAA AGATTCCTGG TATAGATACA CCTGGTGGAC TTGGAGATAT GGGTGATACA
    ACTCCTGTAG AACCGCCGAC TCCCGAGGGT ACCCCTTTGT TGAAGAGGAA AGCGCAACCA
    GATGAAGCAG GACCTGAACA GCCTCCTGCT ATTGAAGAGC CTCCTCCTGA GCCTGAGAAA
    GCAGACATTG AGAATGGTGA GAAGGCTGAG AAGGAAGTGG AGGTCAAGCC TGAGCCGATA
    CCAGAAGAAC CGAAACCTCA GAGAGCCACC AAAGCCAAGA AGGAGAAGAA ATCAGTCAAG
    GGTGCAGGAT TTGAGCTCTG GAATGAGCTG GATGTGCAAA GAAATAAATT CATGAACTGC
    TTATCTCGTA ACTTCTACAA CATTCGTTTC CTGGCTCTGT TCCTTGCCTT TGCCTTGAAC
    TTCATTCTTC TCTTCTACAA GGTATCTGAT ACTCCCCCCA GTTCCGATGA ATTTGAAGGT
    TCTGGTATCT TTGAAGGCTC TGGGCTGTTT GAAGGCTCTG CGGAGGATTT CGAGGGCTCT
    GGTGGAGAGG ATGGAGGTGA TGATGAGGAT GAAGAAGGAC CTGTGTATTA TTTTCTGGAA
    GAAAGCACGG GATACATGCA GCCCACTCTG TCCTTCCTGG CCATTTTGCA CACCGTCATT
    GCTTTCATAT CTATCATTGG ATACAACTGC CTCAAGATTC CACTGGTCAT CTTTAAGAGA
    GAGAAAGAGT TGGCGAGAAA ACTGGAGTTT GATGGTCTTT ACATCACTGA ACAGCCGGCA
    GATGATGACA TTAAAGGCCA GTGGGACAGA CTGGTGTTAA ACACTCCCTC TTTTCCCACC
    AATTACTGGG ACAAATTTGT GAAGCGAAAG GTTCTGGATA AATATGGAGA TATTTATGGA
    CGAGAGAGAA TCGCAGAGCT GCTGGGTATG GATCTGGCAT CTCTGGATGT CAGCAAACAA
    CAGACAGACA AGAAACCAGA AGAGCCAGAC AACTCTATGC TTGCATGGTT CACTTCCATT
    GACATCAAGT ATCAGATCTG GAAATTTGGA GTTGTGTTCA CAGACAATAC TTTCCTCTAC
    CTGGTGTGGT ACACAGTGAT GTCCCTTCTT GGACATTACA ACAACTTTTT CTTTGCTTGT
    CACCTGCTGG ACATCGCAAT GGGTGTCAAG ACACTGCGTA CAATTCTTTC CTCCGTCACA
    CACAATGGAA AACAGCTGAT GATGACTGTG GGTTTGCTGG CTGTGGTTGT GTACCTCTAC
    ACTGTGGTGG CCTTCAACTT CTTCAGGAAG TTCTACAATA AGAGCGAGGA TGAGGATGAG
    CCGGACATGA AGTGTGACGA CATGATGACG TGTTATTTGT TCCACATGTA TGTGGGTGTA
    CGGGCAGGTG GAGGTATAGG AGATGAGATC GAGGACCCTG CTGGAGATGA GTATGAGCTC
    TATCGGGTGG TCTTCGACAT CACCTTCTTC TTCTTTGTCA TCGTCATCCT GCTGGCCATC
    ATTCAGGGTC TGATCATTGA TGCCTTTGGT GAACTGAGAG ACCAGCAGGA GCAAGTGAAA
    GAGGACATGG AGACCAAATG CTTTATATGT GGCATTGGGA GCGACTACTT TGATACAACG
    CCCCATGGCT TTGAGACCCA TACACTTGAG GAACACAACC TGGCCAATTA CATGTTTTTC
    CTGATGTACT TAATCAACAA GGATGAAACA GAGCACACTG GCCAGGAATC ATACGTGTGG
    AAGATGTACC AGGAGAGATG CTGGGATTTC TTCCCTGCCG GAGACTGCTT CAGAAAGCAA
    TATGAAGACC AGCTTGGATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021327993.1
    CDS188..8968
    Translation

    Target ORF information:

    RefSeq Version XM_021472318.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio ryanodine receptor 1a (skeletal) (ryr1a), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021472318.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    ATGATCTACT GGGCTCAAGA GTCTTTCATC CAGAACCCTG AGCTGGTGCG CCTCATGTTT 
    AGTCTGCTAC ACCGGCAGTA TGACGCTTTG GGTGAGCTGA TTCGAGCTTT GCCCAAAGCT
    TATATCATTA ATGCTGTTTC TATACAAGAC ACAATGGAGC TCCTGGAGTG TCTGGGACAG
    ATACGCTCCT TGCTCATCGT TCAGATGGGA CCAGAGGAAG AGAGGCTGAT GATCCAGAGT
    ATCGGAAATA TTATGAGTAA CAGGGTATTT TACCAGCACC CCAACTTGAT GAGAGCTCTG
    GGAATGCATG AGACAGTCAT GGAGGTTATG GTGAACGTAC TCGGAGGCGG AGATTCCAAG
    GAGATCAGAT TCCCTCGAAT GGTCACCAAC TGCTGTCGTT TCCTGTGTTA TTTCTGTCGT
    ATCAGCAGAC AGAATCAGCG CTCGATGTTT GATCATCTGG GCTACCTCCT GCAGAACAGT
    GGCATTGGGC TGGGAATGCG TGGCTCCACT CCTCTTGATG TTGCTGCTGC TTCCTGTATC
    GATAATAATG AGCTTGCCCT GGCTCTCCAA GAGCAAGACC TGGAGATGGT AGTAAAGTAC
    TTGGCAGGCT GTGGTCTGCA GAGCTGCCCT ATGCTCTTGT CAAAGGGTTA CCCGGATATT
    GGATGGAACC CAGTTGGAGG AGAAAAGTAT CTGGATTTCC TGCGCTTTGC TGTGTTTGTG
    AATGGAGAGA GTGTGGAGGA GAACGCTAAT GTGGTGGTCC GGCTGCTGAT CAGGAGGCCT
    GAGTGCTTTG GTCCTGCACT GAGAGGAGAA GGGGGCAATG GTCTGCTGGC TGCCATTGAA
    GAAGCCATCA AGATCTCAGA GGACCCTGCA AGAGATGGAC CCACTGTTAA AAAAGACAGA
    CGCTTCCCGA TGTTTGGAGG TGAAGAGCAG CATGAGGAGA ACAGAGTGCA CCTAGGAAAT
    GCCATCATGT CATTTTACTC AGCTCTTATT GATCTGCTGG GTCGCTGTGC TCCTGAGATG
    CATTTGATCC AGGCTGGTAA AGGTGAAGCT TTGAGAATCA GGGCTATTCT TCGGTCGCTT
    GTGCCCATTG AGGATCTGGT CGGTGTGATC AGCTTGTCAG TCCAGATTCC TGCTTTTGGA
    AAAGATAACA GCATTATTGA GCCGAAGATG TCTGCTAGTT TTGTTCCTGA TCATAAAGCA
    CCAATGGTCC TGTTCCTGGA CAGAGTTTAT GGCATCGACA ATCAGGACTT CCTATTGCAT
    GTACTAGAAG TGGGTTTCCT GCCTGATATG AGGGCGGCAG CCTCCTTAGA CACAGCTGCA
    TTCAGCACTA CAGAAATGGC CTTGGCTCTG AACCGTTACC TGTGCTCCGC CGTGCTTCCT
    CTCATCACTA AATGTGCTCC TTTGTTCGCC GGGACGGACC ACCGAGCCAT CATGATCGAC
    TCCATGCTTC ACACCATCTA CAGGCTGTCT CGAGGACGCT CGCTAACTAA AGCACAAAGA
    GATGTTATTG AGGAATGCCT CATGTCTTTG TGCAGGTATC TGCGCCCTTC CATGCTCCAG
    CACCTTCTCA GGCGGCTAGT ATTTGATGTG CCTATTCTGA ATGAGTATGC CAAAATGCCC
    CTTAAGCTGC TTACCAATCA TTATGAGCGT TGTTGGAAAT ATTATTGCTT GCCTAATGGC
    TGGGCTAACT TTGGCGTATC ATCAGAAGAG GAGCTTCATC TGACCCGTAA ACTCTTTTGG
    GGGATCTTTG AATCTCTGGC TCACAAGAAA TTCGATGCAG AGCTGTTCAA AATCGCCATG
    CCTTGTATAT GTGCGATCGC TGGTGCAATT CCTCCTGATT ATGTGGACGC CAGCTACTCC
    TCCAAAACCG AAAAAAAGGC TTCTGTTGAT GCTGAAGGGA ACTTTGACCC CAAACCTGTT
    GATACCACTA ACACTATAAT TCCCGAGAAG TTGGACGGGT TCATCAACAG ATATGCAGAA
    TATACGCACG ACAGATGGGC TTTTGAAAAA ATTCAAAACA ACTGGACATT TGGTGAGGTG
    ATGGATGAAA ATGCCAAGAC TCATCCCATG CTCAGACCTT ACAAAACATT CTCTGAAAAG
    GATAAAGAAA TCTACCGCTG GCCTATTAAA GAGTCCATCA AGGCCATGAT TGCCTGGGAG
    TGGACACTGG ACAAAGCCAG AGATGGAGAG GATGAGAAAG TTGAGAAGAA AACAGCCCGT
    AAGATCTCAC AGACTGCACA GGCAACCTAT GACCCCAGTC ATGGCTATAG CCCCCAACCT
    ATTGATATCT CAGGCATGAC ACTGTCCAGA GAGCTACAGT CCATGGCAGA GCAGCTCGCA
    GAAAACTACC ACAACACCTG GGGCCGTAAG AAGAAAATGG AATTACAAGC AAAAGGAGGA
    GGTACACACC CTTTGCTTGT GCCTTATGAC ACACTGACGG CTAAAGAAAA GGCACGAGAT
    AGAGAGAAAG CGTATGAGCT ACTCAAGTTT CTGCAGCTCA ATGGATATGC TGTGACAAGA
    GGGCTCAAAG ACATGGAAAG TGACATTTCA TCTATCGAGA AGCGTTTTGC GTATGGCTTC
    CTACAGAAGC TTCTGAAGTG GATGGACATT GCTCAGGAGT TCATAGCACA TCTTGAGGCG
    GTTGTGAGCA GCGGTCGTGT GGAAAAGTCA CCACATGAGC AAGAAATCAA GTTCTTTGCC
    AAGATTCTTT TGCCTTTGAT AAATCAGTAT TTTAAAAACC ACTGCCTTTA CTTCCTCTCC
    ACACCGGCCA AAATCCTAGG CAGCGGCGGA CACGCCTCAA ACAAGGAAAA GGAGATGATT
    GCAAGCATTT TCTGTAAGAT GTCTGCCCTG GTGCGACACA GAGTGTTTCT CTTTGGCACG
    GATGCGCCTG CTATTGTCAA CTGCCTTCAT ATCCTCGCAC GTTCGCTTGA TGCCAGGACG
    GTGATGAAAT CCGGGCCTGA GATTGTGAAA GCAGGACTGC GTTTGTTCTT TGAGAGTGCA
    GCTGATGACA TTGAAAAGAT GGTGGAGAAT CTCAAACTTG GCAAAGTGTC CAAAGGCAAT
    CAGCCAGTGA AAGGTGTGTC CCAGAATATA AACTACACCA CAACGGCTTT GCTTCCTGTC
    CTCACATCAC TGTTTGACCA CATCGCTCAA CACCAATTCG GAGATGATGT TATCCTGGAT
    GATTTGCAGA TGTCCTGCTA CCGAATCATG TGTAGCATCT ACTCTCTTGG GACTGTAAAA
    ACTCCACATG CAGAGAGGCA TAGACCAGCC CTTGGAGAAT GTCTCGCTCA TCTCGCTGCA
    GCAATGCCTG TTGCCTACCT GGAGCCGCAT CTTAATGAAT ACAATGCCTT TTCAGTCTAC
    ACTACCAAGA CACCGAGAGA AAGAGCTATC CTTGGCCTTC CAAACCAAGT TCAAGAGCTG
    TGTTTGGACA TTCCTGAGCT TGACGTTTTA CTTAAGGAGA TCGGAGACCT GGCTGAATCG
    GGAGCTCGAT ACACTGAAAT GCCACATGTG ATTGAGGTCA CTCTGCCCAT GCTGTGTAAC
    TATCTGCCCC GCTGGTGGGA GAGAGGACTG GAGAACTTCC CGGATCTGGA GGGTAAGCTG
    TGCACCGACG TCACCTCTGA ATCCCTCAAC CAGCTTTTGG GAAGCATCAT GAAAATCGTG
    GTCAACAACT TGGGAATCGA CGAAGCCTCC TGGATGAAGA GACTTGCAGT GTTCTCCCAA
    CCCATTGTGA GCCGAGCCAA ACCAGAGATG CTCAAATCCC ACTTCATCCC CACCATGGAG
    AAGCTGAAGA AGCGAACGTC TAAAGTTGTA GCCGAGGAGG AGCATCTGCG TTTGGAGGGA
    AAGTCTGAGG GAGATGAGGA AGAGGGAACC ATCAGAGACG AGTTTGCAGT TCTCTGTAGA
    GACCTCTATG CTCTATATCC TCTTCTCATC CGCTATGTGG ATAACAACAG AGCAAGATGG
    CTGACGTTTC GAGACCCAGA TGCTGAGGAG TTGTTCCGCA TGGTGGGAGA GGTCTTCATC
    TTCTGGTCAA AATCTCACAA TTTCAAGCGA GAGGAGCAAA ACTTTGTGGT GATGAACGAA
    ATCAACAATA TGTCCTTTCT CACTGCTGAC AGTAAGAGCA AGATGAGCAA GGCTGGAGGG
    TCGGATGTGG AGCGTACCAA GAAAAAGAGA CGGGGGGATC GATACTCTGT GCAAACGTCC
    CTCATTGTGG CCGCACTGAA GAAAATGCTT CCCATTGGAC TTAACATGTG TTCTCCTGCA
    GACCAGGAGC TGATTAATCT GGCAAAGACA CGTTATTCTC TGAAAGACAC AGATGAGGAG
    GTCAGAGAGT TCTTGCAAAA TAACCTGCAC CTTCAGGGAA AGGTTGATAA CCCATCTATG
    AGGTGGCAGA TGGCACTGTA TAAGGAAATG GCAGGAAAGG CTGAAGATGC AGATGCCCCA
    GAGAATGTGG TCAAGAGGGT TCAGGAGGTC TCTGCGGTCC TTTACCACAT TGAAGTGACG
    GAGCACCCTT TCAAGTCCAA AAAGATGGTT TGGCACAAAC TGCTGTCGAA GCAGCGGCGT
    AGAGCGGTGG TGGCCTGTTT CAGGATGACT CCACTTTACA ACTTGCCCAG ACACAGAGCA
    TCTAATATGT TCCTGGATGG GTACAAGCGC AACTGGATAA GAAATGAAGG CTATTCCTTT
    GAGGACAGAA TGATTGATGA CCTGTCTAAA GCATTGGAGG AGGAAGAAGA GGAGGAAGAG
    GAAAAGGAGA CAAAGCCAGA CCCTCTTCAT CAGCTCATCC TTCACTTTAG TCGCACAGCA
    CTCACTGAGA AAAGTAAACT GGATATAGAT CACCTATACA TGGCATATGC TGATATCATG
    GCTAAGAGTT GCCACATGGA TGAAGAGGAT GAGGGTGGAG AGGAGGAGAA TGAGGGTGGA
    GAGGAGGAGA TGTCCTTTGA GGAAAAGGAA ATGGAAAAGC AAAGACTGCT CTACCAGCAG
    TCGCGTCTAC ACAACCGAGG GGCTGCTGAA ATGGTCCTGC AGATGATCAG TGCCTGCAAG
    GGTGAGACTG GTTGTATGGT CTCTTCCACC CTAAAACTTG GCATTTCTAT TTTAAATGGC
    GGGAACTGTG AAGTTCAGCA GAAAATGCTG GACTATCTGA AAGACAAGAA AGATGTGGGT
    TTCTTTCTCA GTGTTCAGGC TTTAATGCAG ACCTGCAGTG TTTTGGACCT GAATGCCTTT
    GAGAGGCAGA ATAAAGCTGA GGGTCTAGGT ATGGTGTCAG AAGAGGGTAC AAACATGAAG
    CTAGAACGGG GTGAGAAGGT CATGGCTGAT GACGAGTTCA CTTGCGACCT CTTCCGCTTT
    CTCCAGCTCC TCTGTGAGGG TCACAACAAT GACTTCCAAA ATTACCTCAG AACACAGACA
    GGCAGCACCA CCACCATCAA TGTCATTATC TGTACAGTGG ATTACCTGCT TAGACTACAG
    GAGTCCATCA GTGATTTCTA CTGGTATTAC TCGGGAAAAG ACATCATAGA TGAACCAGGA
    AAGCGAAACT TCTCTAAAGC TATGACTGTG GCCAAACAGG TTTTCAACAG TCTTACCGAG
    TATATCCAGG GTCCCTGCAC AGGAAACCAG CAGTCCCTGG CTCACAGTCG CTTATGGGAT
    GCTGTGGTGG GCTTTCTGCA CGTGTTTGCT CATATGATGA TGAAACTGGC TCAGGGAAGA
    GATTCCAGTC AGATTGGTCT TCTGAAAGAG TTGTTGGACC TTCAGAAAGA CATGGTGGTC
    ATGTTATTGT CCTTGTTGGA GGGTAATGTT GTAAATGGCA CCATTGCTCG CCAGATGGTG
    GACATGCTAG TGGAATCCTC CAGCAATGTA GAAATGATCC TTAAGTTCTT CGACATGTTC
    CTCAAGCTGA AGGACATTGT TGCGTCTGAC GCGTTTCGTG ACTACGTGAC CGACCCTAGA
    GGTTTGATAT CCAAGAAAGA CTTTCAGAAA TCCATGGACA GCCAGAAACA GTACACACCT
    TCAGAGATCC AATTCCTATT GTCATGCTCT GAGGCTGATG AGAATGAGAT GATCAATTAC
    GAGGAGTTTG CAAGTCGCTT TCAAGAGCCG GCCAAAGATA TTGGTTTTAA CATAGCGGTG
    CTGCTCACCA ACCTCTCCGA ACATGTTCCT CACGATACAA GACTGCAGAA CTTCCTCGAA
    CAAGCCGAGA GCGTCCTGAA CTATTTCCGC CCATTCTTGG GTCGCATCGA GATCATGGGC
    GCCAGCAGGA AAATCGAGAG AATCTACTTC GAGATCAGCG AGGTGAATCG CAAGCAGTGG
    GAAATGCCTC AAGTCAAAGA ATCGAAGAGA CAGTTCATAT TCGACGTAGT CAATGAAGGC
    GGCGAATCGG AGAAGATGGA GCTTTTCGTC AACTTCTGCG AGGACACCAT ATTCGAGATG
    AACATCGCGT CGCAGATTTC AGAACAAGAC GAGGAAGAGA AAGAAGAGGA TGATATTGAG
    GAAGCTGAAG GAGGAGATGC TGGTGATGAA GAGGGGGGTG GCGAGGAGGG ACAACCAGAA
    TCAAGCTCTG CATTTGCCGA TTTCATTCAA AGCATCATGA ATTTTCTGGG TATGTTCACC
    TTCAGGAATA TTCGCAGACA GTACCGCAGA CTCCGCAAGA TGACCATTAA GGAGATCGTG
    GTAGGGCTTG CTACCTTCAT GTGGACGATT CTCTTGGGCA TCCTCCATTT CATTTACAGT
    GTTTGTAAAG GCTTCTTCCT CCTCATCTGG CACACGCTGT TTGGAGGAGG TTTGGTTGAA
    GGGGCCAAAA ACATGACAGT AACTGAGTTA CTAGCCAGCA TGCCAGATCC CACACAGGAC
    GAGGTGCATG GGGACTTACC TGGAGAACCG AGGGGAGGAG AGGAACAGGA GACTGGAGGG
    ATAATGGACT CGATGGATGC TGGAGGGGGA GAGGAAGAGG ATGAGGATAT CCAGGAGAAA
    GATGGTGGAA AGATTCCTGG TATAGATACA CCTGGTGGAC TTGGAGATAT GGGTGATACA
    ACTCCTGTAG AACCGCCGAC TCCCGAGGGT ACCCCTTTGT TGAAGAGGAA AGCGCAACCA
    GATGAAGCAG GACCTGAACA GCCTCCTGCT ATTGAAGAGC CTCCTCCTGA GCCTGAGAAA
    GCAGACATTG AGAATGGTGA GAAGGCTGAG AAGGAAGTGG AGGTCAAGCC TGAGCCGATA
    CCAGAAGAAC CGAAACCTCA GAGAGCCACC AAAGCCAAGA AGGAGAAGAA ATCAGTCAAG
    GGTGCAGGAT TTGAGCTCTG GAATGAGCTG GATGTGCAAA GAAATAAATT CATGAACTGC
    TTATCTCGTA ACTTCTACAA CATTCGTTTC CTGGCTCTGT TCCTTGCCTT TGCCTTGAAC
    TTCATTCTTC TCTTCTACAA GGTATCTGAT ACTCCCCCCA GTTCCGATGA ATTTGAAGGT
    TCTGGTATCT TTGAAGGCTC TGGGCTGTTT GAAGGCTCTG CGGAGGATTT CGAGGGCTCT
    GGTGGAGAGG ATGGAGGTGA TGATGAGGAT GAAGAAGGAC CTGTGTATTA TTTTCTGGAA
    GAAAGCACGG GATACATGCA GCCCACTCTG TCCTTCCTGG CCATTTTGCA CACCGTCATT
    GCTTTCATAT CTATCATTGG ATACAACTGC CTCAAGATTC CACTGGTCAT CTTTAAGAGA
    GAGAAAGAGT TGGCGAGAAA ACTGGAGTTT GATGGTCTTT ACATCACTGA ACAGCCGGCA
    GATGATGACA TTAAAGGCCA GTGGGACAGA CTGGTGTTAA ACACTCCCTC TTTTCCCACC
    AATTACTGGG ACAAATTTGT GAAGCGAAAG GTTCTGGATA AATATGGAGA TATTTATGGA
    CGAGAGAGAA TCGCAGAGCT GCTGGGTATG GATCTGGCAT CTCTGGATGT CAGCAAACAA
    CAGACAGACA AGAAACCAGA AGAGCCAGAC AACTCTATGC TTGCATGGTT CACTTCCATT
    GACATCAAGT ATCAGATCTG GAAATTTGGA GTTGTGTTCA CAGACAATAC TTTCCTCTAC
    CTGGTGTGGT ACACAGTGAT GTCCCTTCTT GGACATTACA ACAACTTTTT CTTTGCTTGT
    CACCTGCTGG ACATCGCAAT GGGTGTCAAG ACACTGCGTA CAATTCTTTC CTCCGTCACA
    CACAATGGAA AACAGCTGAT GATGACTGTG GGTTTGCTGG CTGTGGTTGT GTACCTCTAC
    ACTGTGGTGG CCTTCAACTT CTTCAGGAAG TTCTACAATA AGAGCGAGGA TGAGGATGAG
    CCGGACATGA AGTGTGACGA CATGATGACG TGTTATTTGT TCCACATGTA TGTGGGTGTA
    CGGGCAGGTG GAGGTATAGG AGATGAGATC GAGGACCCTG CTGGAGATGA GTATGAGCTC
    TATCGGGTGG TCTTCGACAT CACCTTCTTC TTCTTTGTCA TCGTCATCCT GCTGGCCATC
    ATTCAGGGTC TGATCATTGA TGCCTTTGGT GAACTGAGAG ACCAGCAGGA GCAAGTGAAA
    GAGGACATGG AGACCAAATG CTTTATATGT GGCATTGGGA GCGACTACTT TGATACAACG
    CCCCATGGCT TTGAGACCCA TACACTTGAG GAACACAACC TGGCCAATTA CATGTTTTTC
    CTGATGTACT TAATCAACAA GGATGAAACA GAGCACACTG GCCAGGAATC ATACGTGTGG
    AAGATGTACC AGGAGAGATG CTGGGATTTC TTCCCTGCCG GAGACTGCTT CAGAAAGCAA
    TATGAAGACC AGCTTGGATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa145156
    Clone ID Related Accession (Same CDS sequence) XM_021472319.1
    Accession Version XM_021472319.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8763bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product ryanodine receptor 1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394792.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    1021
    1081
    1141
    1201
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    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
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    1921
    1981
    2041
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    2641
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    3181
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    4141
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    6001
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    6301
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    6421
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    7141
    7201
    7261
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    7381
    7441
    7501
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    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    ATGATCTACT GGGCTCAAGA GTCTTTCATC CAGAACCCTG AGCTGGTGCG CCTCATGTTT 
    AGTCTGCTAC ACCGGCAGTA TGACGCTTTG GGTGAGCTGA TTCGAGCTTT GCCCAAAGCT
    TATATCATTA ATGCTGTTTC TATACAAGAC ACAATGGAGC TCCTGGAGTG TCTGGGACAG
    ATACGCTCCT TGCTCATCGT TCAGATGGGA CCAGAGGAAG AGAGGCTGAT GATCCAGAGT
    ATCGGAAATA TTATGAGTAA CAGGGTATTT TACCAGCACC CCAACTTGAT GAGAGCTCTG
    GGAATGCATG AGACAGTCAT GGAGGTTATG GTGAACGTAC TCGGAGGCGG AGATTCCAAG
    GAGATCAGAT TCCCTCGAAT GGTCACCAAC TGCTGTCGTT TCCTGTGTTA TTTCTGTCGT
    ATCAGCAGAC AGAATCAGCG CTCGATGTTT GATCATCTGG GCTACCTCCT GCAGAACAGT
    GGCATTGGGC TGGGAATGCG TGGCTCCACT CCTCTTGATG TTGCTGCTGC TTCCTGTATC
    GATAATAATG AGCTTGCCCT GGCTCTCCAA GAGCAAGACC TGGAGATGGT AGTAAAGTAC
    TTGGCAGGCT GTGGTCTGCA GAGCTGCCCT ATGCTCTTGT CAAAGGGTTA CCCGGATATT
    GGATGGAACC CAGTTGGAGG AGAAAAGTAT CTGGATTTCC TGCGCTTTGC TGTGTTTGTG
    AATGGAGAGA GTGTGGAGGA GAACGCTAAT GTGGTGGTCC GGCTGCTGAT CAGGAGGCCT
    GAGTGCTTTG GTCCTGCACT GAGAGGAGAA GGGGGCAATG GTCTGCTGGC TGCCATTGAA
    GAAGCCATCA AGATCTCAGA GGACCCTGCA AGAGATGGAC CCACTGTTAA AAAAGACAGA
    CGCTTCCCGA TGTTTGGAGG TGAAGAGCAG CATGAGGAGA ACAGAGTGCA CCTAGGAAAT
    GCCATCATGT CATTTTACTC AGCTCTTATT GATCTGCTGG GTCGCTGTGC TCCTGAGATG
    CATTTGATCC AGGCTGGTAA AGGTGAAGCT TTGAGAATCA GGGCTATTCT TCGGTCGCTT
    GTGCCCATTG AGGATCTGGT CGGTGTGATC AGCTTGTCAG TCCAGATTCC TGCTTTTGGA
    AAAGATAACA GCATTATTGA GCCGAAGATG TCTGCTAGTT TTGTTCCTGA TCATAAAGCA
    CCAATGGTCC TGTTCCTGGA CAGAGTTTAT GGCATCGACA ATCAGGACTT CCTATTGCAT
    GTACTAGAAG TGGGTTTCCT GCCTGATATG AGGGCGGCAG CCTCCTTAGA CACAGCTGCA
    TTCAGCACTA CAGAAATGGC CTTGGCTCTG AACCGTTACC TGTGCTCCGC CGTGCTTCCT
    CTCATCACTA AATGTGCTCC TTTGTTCGCC GGGACGGACC ACCGAGCCAT CATGATCGAC
    TCCATGCTTC ACACCATCTA CAGGCTGTCT CGAGGACGCT CGCTAACTAA AGCACAAAGA
    GATGTTATTG AGGAATGCCT CATGTCTTTG TGCAGGTATC TGCGCCCTTC CATGCTCCAG
    CACCTTCTCA GGCGGCTAGT ATTTGATGTG CCTATTCTGA ATGAGTATGC CAAAATGCCC
    CTTAAGCTGC TTACCAATCA TTATGAGCGT TGTTGGAAAT ATTATTGCTT GCCTAATGGC
    TGGGCTAACT TTGGCGTATC ATCAGAAGAG GAGCTTCATC TGACCCGTAA ACTCTTTTGG
    GGGATCTTTG AATCTCTGGC TCACAAGAAA TTCGATGCAG AGCTGTTCAA AATCGCCATG
    CCTTGTATAT GTGCGATCGC TGGTGCAATT CCTCCTGATT ATGTGGACGC CAGCTACTCC
    TCCAAAACCG AAAAAAAGGC TTCTGTTGAT GCTGAAGGGA ACTTTGACCC CAAACCTGTT
    GATACCACTA ACACTATAAT TCCCGAGAAG TTGGACGGGT TCATCAACAG ATATGCAGAA
    TATACGCACG ACAGATGGGC TTTTGAAAAA ATTCAAAACA ACTGGACATT TGGTGAGGTG
    ATGGATGAAA ATGCCAAGAC TCATCCCATG CTCAGACCTT ACAAAACATT CTCTGAAAAG
    GATAAAGAAA TCTACCGCTG GCCTATTAAA GAGTCCATCA AGGCCATGAT TGCCTGGGAG
    TGGACACTGG ACAAAGCCAG AGATGGAGAG GATGAGAAAG TTGAGAAGAA AACAGCCCGT
    AAGATCTCAC AGACTGCACA GGCAACCTAT GACCCCAGTC ATGGCTATAG CCCCCAACCT
    ATTGATATCT CAGGCATGAC ACTGTCCAGA GAGCTACAGT CCATGGCAGA GCAGCTCGCA
    GAAAACTACC ACAACACCTG GGGCCGTAAG AAGAAAATGG AATTACAAGC AAAAGGAGGA
    GGTACACACC CTTTGCTTGT GCCTTATGAC ACACTGACGG CTAAAGAAAA GGCACGAGAT
    AGAGAGAAAG CGTATGAGCT ACTCAAGTTT CTGCAGCTCA ATGGATATGC TGTGACAAGA
    GGGCTCAAAG ACATGGAAAG TGACATTTCA TCTATCGAGA AGCGTTTTGC GTATGGCTTC
    CTACAGAAGC TTCTGAAGTG GATGGACATT GCTCAGGAGT TCATAGCACA TCTTGAGGCG
    GTTGTGAGCA GCGGTCGTGT GGAAAAGTCA CCACATGAGC AAGAAATCAA GTTCTTTGCC
    AAGATTCTTT TGCCTTTGAT AAATCAGTAT TTTAAAAACC ACTGCCTTTA CTTCCTCTCC
    ACACCGGCCA AAATCCTAGG CAGCGGCGGA CACGCCTCAA ACAAGGAAAA GGAGATGATT
    GCAAGCATTT TCTGTAAGAT GTCTGCCCTG GTGCGACACA GAGTGTTTCT CTTTGGCACG
    GATGCGCCTG CTATTGTCAA CTGCCTTCAT ATCCTCGCAC GTTCGCTTGA TGCCAGGACG
    GTGATGAAAT CCGGGCCTGA GATTGTGAAA GCAGGACTGC GTTTGTTCTT TGAGAGTGCA
    GCTGATGACA TTGAAAAGAT GGTGGAGAAT CTCAAACTTG GCAAAGTGTC CAAAGGCAAT
    CAGCCAGTGA AAGGTGTGTC CCAGAATATA AACTACACCA CAACGGCTTT GCTTCCTGTC
    CTCACATCAC TGTTTGACCA CATCGCTCAA CACCAATTCG GAGATGATGT TATCCTGGAT
    GATTTGCAGA TGTCCTGCTA CCGAATCATG TGTAGCATCT ACTCTCTTGG GACTGTAAAA
    ACTCCACATG CAGAGAGGCA TAGACCAGCC CTTGGAGAAT GTCTCGCTCA TCTCGCTGCA
    GCAATGCCTG TTGCCTACCT GGAGCCGCAT CTTAATGAAT ACAATGCCTT TTCAGTCTAC
    ACTACCAAGA CACCGAGAGA AAGAGCTATC CTTGGCCTTC CAAACCAAGT TCAAGAGCTG
    TGTTTGGACA TTCCTGAGCT TGACGTTTTA CTTAAGGAGA TCGGAGACCT GGCTGAATCG
    GGAGCTCGAT ACACTGAAAT GCCACATGTG ATTGAGGTCA CTCTGCCCAT GCTGTGTAAC
    TATCTGCCCC GCTGGTGGGA GAGAGGACTG GAGAACTTCC CGGATCTGGA GGGTAAGCTG
    TGCACCGACG TCACCTCTGA ATCCCTCAAC CAGCTTTTGG GAAGCATCAT GAAAATCGTG
    GTCAACAACT TGGGAATCGA CGAAGCCTCC TGGATGAAGA GACTTGCAGT GTTCTCCCAA
    CCCATTGTGA GCCGAGCCAA ACCAGAGATG CTCAAATCCC ACTTCATCCC CACCATGGAG
    AAGCTGAAGA AGCGAACGTC TAAAGTTGTA GCCGAGGAGG AGCATCTGCG TTTGGAGGGA
    AAGTCTGAGG GAGATGAGGA AGAGGGAACC ATCAGAGACG AGTTTGCAGT TCTCTGTAGA
    GACCTCTATG CTCTATATCC TCTTCTCATC CGCTATGTGG ATAACAACAG AGCAAGATGG
    CTGACGTTTC GAGACCCAGA TGCTGAGGAG TTGTTCCGCA TGGTGGGAGA GGTCTTCATC
    TTCTGGTCAA AATCTCACAA TTTCAAGCGA GAGGAGCAAA ACTTTGTGGT GATGAACGAA
    ATCAACAATA TGTCCTTTCT CACTGCTGAC AGTAAGAGCA AGATGAGCAA GGCTGGAGGG
    TCGGATGTGG AGCGTACCAA GAAAAAGAGA CGGGGGGATC GATACTCTGT GCAAACGTCC
    CTCATTGTGG CCGCACTGAA GAAAATGCTT CCCATTGGAC TTAACATGTG TTCTCCTGCA
    GACCAGGAGC TGATTAATCT GGCAAAGACA CGTTATTCTC TGAAAGACAC AGATGAGGAG
    GTCAGAGAGT TCTTGCAAAA TAACCTGCAC CTTCAGGGAA AGGTTGATAA CCCATCTATG
    AGGTGGCAGA TGGCACTGTA TAAGGAAATG GCAGGAAAGG CTGAAGATGC AGATGCCCCA
    GAGAATGTGG TCAAGAGGGT TCAGGAGGTC TCTGCGGTCC TTTACCACAT TGAAGTGACG
    GAGCACCCTT TCAAGTCCAA AAAGATGGTT TGGCACAAAC TGCTGTCGAA GCAGCGGCGT
    AGAGCGGTGG TGGCCTGTTT CAGGATGACT CCACTTTACA ACTTGCCCAG ACACAGAGCA
    TCTAATATGT TCCTGGATGG GTACAAGCGC AACTGGATAA GAAATGAAGG CTATTCCTTT
    GAGGACAGAA TGATTGATGA CCTGTCTAAA GCATTGGAGG AGGAAGAAGA GGAGGAAGAG
    GAAAAGGAGA CAAAGCCAGA CCCTCTTCAT CAGCTCATCC TTCACTTTAG TCGCACAGCA
    CTCACTGAGA AAAGTAAACT GGATATAGAT CACCTATACA TGGCATATGC TGATATCATG
    GCTAAGAGTT GCCACATGGA TGAAGAGGAT GAGGGTGGAG AGGAGGAGAA TGAGGGTGGA
    GAGGAGGAGA TGTCCTTTGA GGAAAAGGAA ATGGAAAAGC AAAGACTGCT CTACCAGCAG
    TCGCGTCTAC ACAACCGAGG GGCTGCTGAA ATGGTCCTGC AGATGATCAG TGCCTGCAAG
    GGTGAGACTG GTTGTATGGT CTCTTCCACC CTAAAACTTG GCATTTCTAT TTTAAATGGC
    GGGAACTGTG AAGTTCAGCA GAAAATGCTG GACTATCTGA AAGACAAGAA AGATGTGGGT
    TTCTTTCTCA GTGTTCAGGC TTTAATGCAG ACCTGCAGTG TTTTGGACCT GAATGCCTTT
    GAGAGGCAGA ATAAAGCTGA GGGTCTAGGT ATGGTGTCAG AAGAGGGTAC AAGTGAGAAG
    GTCATGGCTG ATGACGAGTT CACTTGCGAC CTCTTCCGCT TTCTCCAGCT CCTCTGTGAG
    GGTCACAACA ATGACTTCCA AAATTACCTC AGAACACAGA CAGGCAGCAC CACCACCATC
    AATGTCATTA TCTGTACAGT GGATTACCTG CTTAGACTAC AGGAGTCCAT CAGTGATTTC
    TACTGGTATT ACTCGGGAAA AGACATCATA GATGAACCAG GAAAGCGAAA CTTCTCTAAA
    GCTATGACTG TGGCCAAACA GGTTTTCAAC AGTCTTACCG AGTATATCCA GGGTCCCTGC
    ACAGGAAACC AGCAGTCCCT GGCTCACAGT CGCTTATGGG ATGCTGTGGT GGGCTTTCTG
    CACGTGTTTG CTCATATGAT GATGAAACTG GCTCAGGGAA GAGATTCCAG TCAGATTGGT
    CTTCTGAAAG AGTTGTTGGA CCTTCAGAAA GACATGGTGG TCATGTTATT GTCCTTGTTG
    GAGGGTAATG TTGTAAATGG CACCATTGCT CGCCAGATGG TGGACATGCT AGTGGAATCC
    TCCAGCAATG TAGAAATGAT CCTTAAGTTC TTCGACATGT TCCTCAAGCT GAAGGACATT
    GTTGCGTCTG ACGCGTTTCG TGACTACGTG ACCGACCCTA GAGGTTTGAT ATCCAAGAAA
    GACTTTCAGA AATCCATGGA CAGCCAGAAA CAGTACACAC CTTCAGAGAT CCAATTCCTA
    TTGTCATGCT CTGAGGCTGA TGAGAATGAG ATGATCAATT ACGAGGAGTT TGCAAGTCGC
    TTTCAAGAGC CGGCCAAAGA TATTGGTTTT AACATAGCGG TGCTGCTCAC CAACCTCTCC
    GAACATGTTC CTCACGATAC AAGACTGCAG AACTTCCTCG AACAAGCCGA GAGCGTCCTG
    AACTATTTCC GCCCATTCTT GGGTCGCATC GAGATCATGG GCGCCAGCAG GAAAATCGAG
    AGAATCTACT TCGAGATCAG CGAGGTGAAT CGCAAGCAGT GGGAAATGCC TCAAGTCAAA
    GAATCGAAGA GACAGTTCAT ATTCGACGTA GTCAATGAAG GCGGCGAATC GGAGAAGATG
    GAGCTTTTCG TCAACTTCTG CGAGGACACC ATATTCGAGA TGAACATCGC GTCGCAGATT
    TCAGAACAAG ACGAGGAAGA GAAAGAAGAG GATGATATTG AGGAAGCTGA AGGAGGAGAT
    GCTGGTGATG AAGAGGGGGG TGGCGAGGAG GGACAACCAG AATCAAGCTC TGCATTTGCC
    GATTTCATTC AAAGCATCAT GAATTTTCTG GGTATGTTCA CCTTCAGGAA TATTCGCAGA
    CAGTACCGCA GACTCCGCAA GATGACCATT AAGGAGATCG TGGTAGGGCT TGCTACCTTC
    ATGTGGACGA TTCTCTTGGG CATCCTCCAT TTCATTTACA GTGTTTGTAA AGGCTTCTTC
    CTCCTCATCT GGCACACGCT GTTTGGAGGA GGTTTGGTTG AAGGGGCCAA AAACATGACA
    GTAACTGAGT TACTAGCCAG CATGCCAGAT CCCACACAGG ACGAGGTGCA TGGGGACTTA
    CCTGGAGAAC CGAGGGGAGG AGAGGAACAG GAGACTGGAG GGATAATGGA CTCGATGGAT
    GCTGGAGGGG GAGAGGAAGA GGATGAGGAT ATCCAGGAGA AAGATGGTGG AAAGATTCCT
    GGTATAGATA CACCTGGTGG ACTTGGAGAT ATGGGTGATA CAACTCCTGT AGAACCGCCG
    ACTCCCGAGG GTACCCCTTT GTTGAAGAGG AAAGCGCAAC CAGATGAAGC AGGACCTGAA
    CAGCCTCCTG CTATTGAAGA GCCTCCTCCT GAGCCTGAGA AAGCAGACAT TGAGAATGGT
    GAGAAGGCTG AGAAGGAAGT GGAGGTCAAG CCTGAGCCGA TACCAGAAGA ACCGAAACCT
    CAGAGAGCCA CCAAAGCCAA GAAGGAGAAG AAATCAGTCA AGGGTGCAGG ATTTGAGCTC
    TGGAATGAGC TGGATGTGCA AAGAAATAAA TTCATGAACT GCTTATCTCG TAACTTCTAC
    AACATTCGTT TCCTGGCTCT GTTCCTTGCC TTTGCCTTGA ACTTCATTCT TCTCTTCTAC
    AAGGTATCTG ATACTCCCCC CAGTTCCGAT GAATTTGAAG GTTCTGGTAT CTTTGAAGGC
    TCTGGGCTGT TTGAAGGCTC TGCGGAGGAT TTCGAGGGCT CTGGTGGAGA GGATGGAGGT
    GATGATGAGG ATGAAGAAGG ACCTGTGTAT TATTTTCTGG AAGAAAGCAC GGGATACATG
    CAGCCCACTC TGTCCTTCCT GGCCATTTTG CACACCGTCA TTGCTTTCAT ATCTATCATT
    GGATACAACT GCCTCAAGAT TCCACTGGTC ATCTTTAAGA GAGAGAAAGA GTTGGCGAGA
    AAACTGGAGT TTGATGGTCT TTACATCACT GAACAGCCGG CAGATGATGA CATTAAAGGC
    CAGTGGGACA GACTGGTGTT AAACACTCCC TCTTTTCCCA CCAATTACTG GGACAAATTT
    GTGAAGCGAA AGGTTCTGGA TAAATATGGA GATATTTATG GACGAGAGAG AATCGCAGAG
    CTGCTGGGTA TGGATCTGGC ATCTCTGGAT GTCAGCAAAC AACAGACAGA CAAGAAACCA
    GAAGAGCCAG ACAACTCTAT GCTTGCATGG TTCACTTCCA TTGACATCAA GTATCAGATC
    TGGAAATTTG GAGTTGTGTT CACAGACAAT ACTTTCCTCT ACCTGGTGTG GTACACAGTG
    ATGTCCCTTC TTGGACATTA CAACAACTTT TTCTTTGCTT GTCACCTGCT GGACATCGCA
    ATGGGTGTCA AGACACTGCG TACAATTCTT TCCTCCGTCA CACACAATGG AAAACAGCTG
    ATGATGACTG TGGGTTTGCT GGCTGTGGTT GTGTACCTCT ACACTGTGGT GGCCTTCAAC
    TTCTTCAGGA AGTTCTACAA TAAGAGCGAG GATGAGGATG AGCCGGACAT GAAGTGTGAC
    GACATGATGA CGTGTTATTT GTTCCACATG TATGTGGGTG TACGGGCAGG TGGAGGTATA
    GGAGATGAGA TCGAGGACCC TGCTGGAGAT GAGTATGAGC TCTATCGGGT GGTCTTCGAC
    ATCACCTTCT TCTTCTTTGT CATCGTCATC CTGCTGGCCA TCATTCAGGG TCTGATCATT
    GATGCCTTTG GTGAACTGAG AGACCAGCAG GAGCAAGTGA AAGAGGACAT GGAGACCAAA
    TGCTTTATAT GTGGCATTGG GAGCGACTAC TTTGATACAA CGCCCCATGG CTTTGAGACC
    CATACACTTG AGGAACACAA CCTGGCCAAT TACATGTTTT TCCTGATGTA CTTAATCAAC
    AAGGATGAAA CAGAGCACAC TGGCCAGGAA TCATACGTGT GGAAGATGTA CCAGGAGAGA
    TGCTGGGATT TCTTCCCTGC CGGAGACTGC TTCAGAAAGC AATATGAAGA CCAGCTTGGA
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021327994.1
    CDS188..8950
    Translation

    Target ORF information:

    RefSeq Version XM_021472319.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio ryanodine receptor 1a (skeletal) (ryr1a), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021472319.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    ATGATCTACT GGGCTCAAGA GTCTTTCATC CAGAACCCTG AGCTGGTGCG CCTCATGTTT 
    AGTCTGCTAC ACCGGCAGTA TGACGCTTTG GGTGAGCTGA TTCGAGCTTT GCCCAAAGCT
    TATATCATTA ATGCTGTTTC TATACAAGAC ACAATGGAGC TCCTGGAGTG TCTGGGACAG
    ATACGCTCCT TGCTCATCGT TCAGATGGGA CCAGAGGAAG AGAGGCTGAT GATCCAGAGT
    ATCGGAAATA TTATGAGTAA CAGGGTATTT TACCAGCACC CCAACTTGAT GAGAGCTCTG
    GGAATGCATG AGACAGTCAT GGAGGTTATG GTGAACGTAC TCGGAGGCGG AGATTCCAAG
    GAGATCAGAT TCCCTCGAAT GGTCACCAAC TGCTGTCGTT TCCTGTGTTA TTTCTGTCGT
    ATCAGCAGAC AGAATCAGCG CTCGATGTTT GATCATCTGG GCTACCTCCT GCAGAACAGT
    GGCATTGGGC TGGGAATGCG TGGCTCCACT CCTCTTGATG TTGCTGCTGC TTCCTGTATC
    GATAATAATG AGCTTGCCCT GGCTCTCCAA GAGCAAGACC TGGAGATGGT AGTAAAGTAC
    TTGGCAGGCT GTGGTCTGCA GAGCTGCCCT ATGCTCTTGT CAAAGGGTTA CCCGGATATT
    GGATGGAACC CAGTTGGAGG AGAAAAGTAT CTGGATTTCC TGCGCTTTGC TGTGTTTGTG
    AATGGAGAGA GTGTGGAGGA GAACGCTAAT GTGGTGGTCC GGCTGCTGAT CAGGAGGCCT
    GAGTGCTTTG GTCCTGCACT GAGAGGAGAA GGGGGCAATG GTCTGCTGGC TGCCATTGAA
    GAAGCCATCA AGATCTCAGA GGACCCTGCA AGAGATGGAC CCACTGTTAA AAAAGACAGA
    CGCTTCCCGA TGTTTGGAGG TGAAGAGCAG CATGAGGAGA ACAGAGTGCA CCTAGGAAAT
    GCCATCATGT CATTTTACTC AGCTCTTATT GATCTGCTGG GTCGCTGTGC TCCTGAGATG
    CATTTGATCC AGGCTGGTAA AGGTGAAGCT TTGAGAATCA GGGCTATTCT TCGGTCGCTT
    GTGCCCATTG AGGATCTGGT CGGTGTGATC AGCTTGTCAG TCCAGATTCC TGCTTTTGGA
    AAAGATAACA GCATTATTGA GCCGAAGATG TCTGCTAGTT TTGTTCCTGA TCATAAAGCA
    CCAATGGTCC TGTTCCTGGA CAGAGTTTAT GGCATCGACA ATCAGGACTT CCTATTGCAT
    GTACTAGAAG TGGGTTTCCT GCCTGATATG AGGGCGGCAG CCTCCTTAGA CACAGCTGCA
    TTCAGCACTA CAGAAATGGC CTTGGCTCTG AACCGTTACC TGTGCTCCGC CGTGCTTCCT
    CTCATCACTA AATGTGCTCC TTTGTTCGCC GGGACGGACC ACCGAGCCAT CATGATCGAC
    TCCATGCTTC ACACCATCTA CAGGCTGTCT CGAGGACGCT CGCTAACTAA AGCACAAAGA
    GATGTTATTG AGGAATGCCT CATGTCTTTG TGCAGGTATC TGCGCCCTTC CATGCTCCAG
    CACCTTCTCA GGCGGCTAGT ATTTGATGTG CCTATTCTGA ATGAGTATGC CAAAATGCCC
    CTTAAGCTGC TTACCAATCA TTATGAGCGT TGTTGGAAAT ATTATTGCTT GCCTAATGGC
    TGGGCTAACT TTGGCGTATC ATCAGAAGAG GAGCTTCATC TGACCCGTAA ACTCTTTTGG
    GGGATCTTTG AATCTCTGGC TCACAAGAAA TTCGATGCAG AGCTGTTCAA AATCGCCATG
    CCTTGTATAT GTGCGATCGC TGGTGCAATT CCTCCTGATT ATGTGGACGC CAGCTACTCC
    TCCAAAACCG AAAAAAAGGC TTCTGTTGAT GCTGAAGGGA ACTTTGACCC CAAACCTGTT
    GATACCACTA ACACTATAAT TCCCGAGAAG TTGGACGGGT TCATCAACAG ATATGCAGAA
    TATACGCACG ACAGATGGGC TTTTGAAAAA ATTCAAAACA ACTGGACATT TGGTGAGGTG
    ATGGATGAAA ATGCCAAGAC TCATCCCATG CTCAGACCTT ACAAAACATT CTCTGAAAAG
    GATAAAGAAA TCTACCGCTG GCCTATTAAA GAGTCCATCA AGGCCATGAT TGCCTGGGAG
    TGGACACTGG ACAAAGCCAG AGATGGAGAG GATGAGAAAG TTGAGAAGAA AACAGCCCGT
    AAGATCTCAC AGACTGCACA GGCAACCTAT GACCCCAGTC ATGGCTATAG CCCCCAACCT
    ATTGATATCT CAGGCATGAC ACTGTCCAGA GAGCTACAGT CCATGGCAGA GCAGCTCGCA
    GAAAACTACC ACAACACCTG GGGCCGTAAG AAGAAAATGG AATTACAAGC AAAAGGAGGA
    GGTACACACC CTTTGCTTGT GCCTTATGAC ACACTGACGG CTAAAGAAAA GGCACGAGAT
    AGAGAGAAAG CGTATGAGCT ACTCAAGTTT CTGCAGCTCA ATGGATATGC TGTGACAAGA
    GGGCTCAAAG ACATGGAAAG TGACATTTCA TCTATCGAGA AGCGTTTTGC GTATGGCTTC
    CTACAGAAGC TTCTGAAGTG GATGGACATT GCTCAGGAGT TCATAGCACA TCTTGAGGCG
    GTTGTGAGCA GCGGTCGTGT GGAAAAGTCA CCACATGAGC AAGAAATCAA GTTCTTTGCC
    AAGATTCTTT TGCCTTTGAT AAATCAGTAT TTTAAAAACC ACTGCCTTTA CTTCCTCTCC
    ACACCGGCCA AAATCCTAGG CAGCGGCGGA CACGCCTCAA ACAAGGAAAA GGAGATGATT
    GCAAGCATTT TCTGTAAGAT GTCTGCCCTG GTGCGACACA GAGTGTTTCT CTTTGGCACG
    GATGCGCCTG CTATTGTCAA CTGCCTTCAT ATCCTCGCAC GTTCGCTTGA TGCCAGGACG
    GTGATGAAAT CCGGGCCTGA GATTGTGAAA GCAGGACTGC GTTTGTTCTT TGAGAGTGCA
    GCTGATGACA TTGAAAAGAT GGTGGAGAAT CTCAAACTTG GCAAAGTGTC CAAAGGCAAT
    CAGCCAGTGA AAGGTGTGTC CCAGAATATA AACTACACCA CAACGGCTTT GCTTCCTGTC
    CTCACATCAC TGTTTGACCA CATCGCTCAA CACCAATTCG GAGATGATGT TATCCTGGAT
    GATTTGCAGA TGTCCTGCTA CCGAATCATG TGTAGCATCT ACTCTCTTGG GACTGTAAAA
    ACTCCACATG CAGAGAGGCA TAGACCAGCC CTTGGAGAAT GTCTCGCTCA TCTCGCTGCA
    GCAATGCCTG TTGCCTACCT GGAGCCGCAT CTTAATGAAT ACAATGCCTT TTCAGTCTAC
    ACTACCAAGA CACCGAGAGA AAGAGCTATC CTTGGCCTTC CAAACCAAGT TCAAGAGCTG
    TGTTTGGACA TTCCTGAGCT TGACGTTTTA CTTAAGGAGA TCGGAGACCT GGCTGAATCG
    GGAGCTCGAT ACACTGAAAT GCCACATGTG ATTGAGGTCA CTCTGCCCAT GCTGTGTAAC
    TATCTGCCCC GCTGGTGGGA GAGAGGACTG GAGAACTTCC CGGATCTGGA GGGTAAGCTG
    TGCACCGACG TCACCTCTGA ATCCCTCAAC CAGCTTTTGG GAAGCATCAT GAAAATCGTG
    GTCAACAACT TGGGAATCGA CGAAGCCTCC TGGATGAAGA GACTTGCAGT GTTCTCCCAA
    CCCATTGTGA GCCGAGCCAA ACCAGAGATG CTCAAATCCC ACTTCATCCC CACCATGGAG
    AAGCTGAAGA AGCGAACGTC TAAAGTTGTA GCCGAGGAGG AGCATCTGCG TTTGGAGGGA
    AAGTCTGAGG GAGATGAGGA AGAGGGAACC ATCAGAGACG AGTTTGCAGT TCTCTGTAGA
    GACCTCTATG CTCTATATCC TCTTCTCATC CGCTATGTGG ATAACAACAG AGCAAGATGG
    CTGACGTTTC GAGACCCAGA TGCTGAGGAG TTGTTCCGCA TGGTGGGAGA GGTCTTCATC
    TTCTGGTCAA AATCTCACAA TTTCAAGCGA GAGGAGCAAA ACTTTGTGGT GATGAACGAA
    ATCAACAATA TGTCCTTTCT CACTGCTGAC AGTAAGAGCA AGATGAGCAA GGCTGGAGGG
    TCGGATGTGG AGCGTACCAA GAAAAAGAGA CGGGGGGATC GATACTCTGT GCAAACGTCC
    CTCATTGTGG CCGCACTGAA GAAAATGCTT CCCATTGGAC TTAACATGTG TTCTCCTGCA
    GACCAGGAGC TGATTAATCT GGCAAAGACA CGTTATTCTC TGAAAGACAC AGATGAGGAG
    GTCAGAGAGT TCTTGCAAAA TAACCTGCAC CTTCAGGGAA AGGTTGATAA CCCATCTATG
    AGGTGGCAGA TGGCACTGTA TAAGGAAATG GCAGGAAAGG CTGAAGATGC AGATGCCCCA
    GAGAATGTGG TCAAGAGGGT TCAGGAGGTC TCTGCGGTCC TTTACCACAT TGAAGTGACG
    GAGCACCCTT TCAAGTCCAA AAAGATGGTT TGGCACAAAC TGCTGTCGAA GCAGCGGCGT
    AGAGCGGTGG TGGCCTGTTT CAGGATGACT CCACTTTACA ACTTGCCCAG ACACAGAGCA
    TCTAATATGT TCCTGGATGG GTACAAGCGC AACTGGATAA GAAATGAAGG CTATTCCTTT
    GAGGACAGAA TGATTGATGA CCTGTCTAAA GCATTGGAGG AGGAAGAAGA GGAGGAAGAG
    GAAAAGGAGA CAAAGCCAGA CCCTCTTCAT CAGCTCATCC TTCACTTTAG TCGCACAGCA
    CTCACTGAGA AAAGTAAACT GGATATAGAT CACCTATACA TGGCATATGC TGATATCATG
    GCTAAGAGTT GCCACATGGA TGAAGAGGAT GAGGGTGGAG AGGAGGAGAA TGAGGGTGGA
    GAGGAGGAGA TGTCCTTTGA GGAAAAGGAA ATGGAAAAGC AAAGACTGCT CTACCAGCAG
    TCGCGTCTAC ACAACCGAGG GGCTGCTGAA ATGGTCCTGC AGATGATCAG TGCCTGCAAG
    GGTGAGACTG GTTGTATGGT CTCTTCCACC CTAAAACTTG GCATTTCTAT TTTAAATGGC
    GGGAACTGTG AAGTTCAGCA GAAAATGCTG GACTATCTGA AAGACAAGAA AGATGTGGGT
    TTCTTTCTCA GTGTTCAGGC TTTAATGCAG ACCTGCAGTG TTTTGGACCT GAATGCCTTT
    GAGAGGCAGA ATAAAGCTGA GGGTCTAGGT ATGGTGTCAG AAGAGGGTAC AAGTGAGAAG
    GTCATGGCTG ATGACGAGTT CACTTGCGAC CTCTTCCGCT TTCTCCAGCT CCTCTGTGAG
    GGTCACAACA ATGACTTCCA AAATTACCTC AGAACACAGA CAGGCAGCAC CACCACCATC
    AATGTCATTA TCTGTACAGT GGATTACCTG CTTAGACTAC AGGAGTCCAT CAGTGATTTC
    TACTGGTATT ACTCGGGAAA AGACATCATA GATGAACCAG GAAAGCGAAA CTTCTCTAAA
    GCTATGACTG TGGCCAAACA GGTTTTCAAC AGTCTTACCG AGTATATCCA GGGTCCCTGC
    ACAGGAAACC AGCAGTCCCT GGCTCACAGT CGCTTATGGG ATGCTGTGGT GGGCTTTCTG
    CACGTGTTTG CTCATATGAT GATGAAACTG GCTCAGGGAA GAGATTCCAG TCAGATTGGT
    CTTCTGAAAG AGTTGTTGGA CCTTCAGAAA GACATGGTGG TCATGTTATT GTCCTTGTTG
    GAGGGTAATG TTGTAAATGG CACCATTGCT CGCCAGATGG TGGACATGCT AGTGGAATCC
    TCCAGCAATG TAGAAATGAT CCTTAAGTTC TTCGACATGT TCCTCAAGCT GAAGGACATT
    GTTGCGTCTG ACGCGTTTCG TGACTACGTG ACCGACCCTA GAGGTTTGAT ATCCAAGAAA
    GACTTTCAGA AATCCATGGA CAGCCAGAAA CAGTACACAC CTTCAGAGAT CCAATTCCTA
    TTGTCATGCT CTGAGGCTGA TGAGAATGAG ATGATCAATT ACGAGGAGTT TGCAAGTCGC
    TTTCAAGAGC CGGCCAAAGA TATTGGTTTT AACATAGCGG TGCTGCTCAC CAACCTCTCC
    GAACATGTTC CTCACGATAC AAGACTGCAG AACTTCCTCG AACAAGCCGA GAGCGTCCTG
    AACTATTTCC GCCCATTCTT GGGTCGCATC GAGATCATGG GCGCCAGCAG GAAAATCGAG
    AGAATCTACT TCGAGATCAG CGAGGTGAAT CGCAAGCAGT GGGAAATGCC TCAAGTCAAA
    GAATCGAAGA GACAGTTCAT ATTCGACGTA GTCAATGAAG GCGGCGAATC GGAGAAGATG
    GAGCTTTTCG TCAACTTCTG CGAGGACACC ATATTCGAGA TGAACATCGC GTCGCAGATT
    TCAGAACAAG ACGAGGAAGA GAAAGAAGAG GATGATATTG AGGAAGCTGA AGGAGGAGAT
    GCTGGTGATG AAGAGGGGGG TGGCGAGGAG GGACAACCAG AATCAAGCTC TGCATTTGCC
    GATTTCATTC AAAGCATCAT GAATTTTCTG GGTATGTTCA CCTTCAGGAA TATTCGCAGA
    CAGTACCGCA GACTCCGCAA GATGACCATT AAGGAGATCG TGGTAGGGCT TGCTACCTTC
    ATGTGGACGA TTCTCTTGGG CATCCTCCAT TTCATTTACA GTGTTTGTAA AGGCTTCTTC
    CTCCTCATCT GGCACACGCT GTTTGGAGGA GGTTTGGTTG AAGGGGCCAA AAACATGACA
    GTAACTGAGT TACTAGCCAG CATGCCAGAT CCCACACAGG ACGAGGTGCA TGGGGACTTA
    CCTGGAGAAC CGAGGGGAGG AGAGGAACAG GAGACTGGAG GGATAATGGA CTCGATGGAT
    GCTGGAGGGG GAGAGGAAGA GGATGAGGAT ATCCAGGAGA AAGATGGTGG AAAGATTCCT
    GGTATAGATA CACCTGGTGG ACTTGGAGAT ATGGGTGATA CAACTCCTGT AGAACCGCCG
    ACTCCCGAGG GTACCCCTTT GTTGAAGAGG AAAGCGCAAC CAGATGAAGC AGGACCTGAA
    CAGCCTCCTG CTATTGAAGA GCCTCCTCCT GAGCCTGAGA AAGCAGACAT TGAGAATGGT
    GAGAAGGCTG AGAAGGAAGT GGAGGTCAAG CCTGAGCCGA TACCAGAAGA ACCGAAACCT
    CAGAGAGCCA CCAAAGCCAA GAAGGAGAAG AAATCAGTCA AGGGTGCAGG ATTTGAGCTC
    TGGAATGAGC TGGATGTGCA AAGAAATAAA TTCATGAACT GCTTATCTCG TAACTTCTAC
    AACATTCGTT TCCTGGCTCT GTTCCTTGCC TTTGCCTTGA ACTTCATTCT TCTCTTCTAC
    AAGGTATCTG ATACTCCCCC CAGTTCCGAT GAATTTGAAG GTTCTGGTAT CTTTGAAGGC
    TCTGGGCTGT TTGAAGGCTC TGCGGAGGAT TTCGAGGGCT CTGGTGGAGA GGATGGAGGT
    GATGATGAGG ATGAAGAAGG ACCTGTGTAT TATTTTCTGG AAGAAAGCAC GGGATACATG
    CAGCCCACTC TGTCCTTCCT GGCCATTTTG CACACCGTCA TTGCTTTCAT ATCTATCATT
    GGATACAACT GCCTCAAGAT TCCACTGGTC ATCTTTAAGA GAGAGAAAGA GTTGGCGAGA
    AAACTGGAGT TTGATGGTCT TTACATCACT GAACAGCCGG CAGATGATGA CATTAAAGGC
    CAGTGGGACA GACTGGTGTT AAACACTCCC TCTTTTCCCA CCAATTACTG GGACAAATTT
    GTGAAGCGAA AGGTTCTGGA TAAATATGGA GATATTTATG GACGAGAGAG AATCGCAGAG
    CTGCTGGGTA TGGATCTGGC ATCTCTGGAT GTCAGCAAAC AACAGACAGA CAAGAAACCA
    GAAGAGCCAG ACAACTCTAT GCTTGCATGG TTCACTTCCA TTGACATCAA GTATCAGATC
    TGGAAATTTG GAGTTGTGTT CACAGACAAT ACTTTCCTCT ACCTGGTGTG GTACACAGTG
    ATGTCCCTTC TTGGACATTA CAACAACTTT TTCTTTGCTT GTCACCTGCT GGACATCGCA
    ATGGGTGTCA AGACACTGCG TACAATTCTT TCCTCCGTCA CACACAATGG AAAACAGCTG
    ATGATGACTG TGGGTTTGCT GGCTGTGGTT GTGTACCTCT ACACTGTGGT GGCCTTCAAC
    TTCTTCAGGA AGTTCTACAA TAAGAGCGAG GATGAGGATG AGCCGGACAT GAAGTGTGAC
    GACATGATGA CGTGTTATTT GTTCCACATG TATGTGGGTG TACGGGCAGG TGGAGGTATA
    GGAGATGAGA TCGAGGACCC TGCTGGAGAT GAGTATGAGC TCTATCGGGT GGTCTTCGAC
    ATCACCTTCT TCTTCTTTGT CATCGTCATC CTGCTGGCCA TCATTCAGGG TCTGATCATT
    GATGCCTTTG GTGAACTGAG AGACCAGCAG GAGCAAGTGA AAGAGGACAT GGAGACCAAA
    TGCTTTATAT GTGGCATTGG GAGCGACTAC TTTGATACAA CGCCCCATGG CTTTGAGACC
    CATACACTTG AGGAACACAA CCTGGCCAAT TACATGTTTT TCCTGATGTA CTTAATCAAC
    AAGGATGAAA CAGAGCACAC TGGCCAGGAA TCATACGTGT GGAAGATGTA CCAGGAGAGA
    TGCTGGGATT TCTTCCCTGC CGGAGACTGC TTCAGAAAGC AATATGAAGA CCAGCTTGGA
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa145157
    Clone ID Related Accession (Same CDS sequence) XM_021472320.1
    Accession Version XM_021472320.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8775bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product ryanodine receptor 1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394792.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    ATGATCTACT GGGCTCAAGA GTCTTTCATC CAGAACCCTG AGCTGGTGCG CCTCATGTTT 
    AGTCTGCTAC ACCGGCAGTA TGACGCTTTG GGTGAGCTGA TTCGAGCTTT GCCCAAAGCT
    TATATCATTA ATGCTGTTTC TATACAAGAC ACAATGGAGC TCCTGGAGTG TCTGGGACAG
    ATACGCTCCT TGCTCATCGT TCAGATGGGA CCAGAGGAAG AGAGGCTGAT GATCCAGAGT
    ATCGGAAATA TTATGAGTAA CAGGGTATTT TACCAGCACC CCAACTTGAT GAGAGCTCTG
    GGAATGCATG AGACAGTCAT GGAGGTTATG GTGAACGTAC TCGGAGGCGG AGATTCCAAG
    GAGATCAGAT TCCCTCGAAT GGTCACCAAC TGCTGTCGTT TCCTGTGTTA TTTCTGTCGT
    ATCAGCAGAC AGAATCAGCG CTCGATGTTT GATCATCTGG GCTACCTCCT GCAGAACAGT
    GGCATTGGGC TGGGAATGCG TGGCTCCACT CCTCTTGATG TTGCTGCTGC TTCCTGTATC
    GATAATAATG AGCTTGCCCT GGCTCTCCAA GAGCAAGACC TGGAGATGGT AGTAAAGTAC
    TTGGCAGGCT GTGGTCTGCA GAGCTGCCCT ATGCTCTTGT CAAAGGGTTA CCCGGATATT
    GGATGGAACC CAGTTGGAGG AGAAAAGTAT CTGGATTTCC TGCGCTTTGC TGTGTTTGTG
    AATGGAGAGA GTGTGGAGGA GAACGCTAAT GTGGTGGTCC GGCTGCTGAT CAGGAGGCCT
    GAGTGCTTTG GTCCTGCACT GAGAGGAGAA GGGGGCAATG GTCTGCTGGC TGCCATTGAA
    GAAGCCATCA AGATCTCAGA GGACCCTGCA AGAGATGGAC CCACTGTTAA AAAAGACAGA
    CGCTTCCCGA TGTTTGGAGG TGAAGAGCAG CATGAGGAGA ACAGAGTGCA CCTAGGAAAT
    GCCATCATGT CATTTTACTC AGCTCTTATT GATCTGCTGG GTCGCTGTGC TCCTGAGATG
    CATTTGATCC AGGCTGGTAA AGGTGAAGCT TTGAGAATCA GGGCTATTCT TCGGTCGCTT
    GTGCCCATTG AGGATCTGGT CGGTGTGATC AGCTTGTCAG TCCAGATTCC TGCTTTTGGA
    AAAGATAACA GCATTATTGA GCCGAAGATG TCTGCTAGTT TTGTTCCTGA TCATAAAGCA
    CCAATGGTCC TGTTCCTGGA CAGAGTTTAT GGCATCGACA ATCAGGACTT CCTATTGCAT
    GTACTAGAAG TGGGTTTCCT GCCTGATATG AGGGCGGCAG CCTCCTTAGA CACAGCTGCA
    TTCAGCACTA CAGAAATGGC CTTGGCTCTG AACCGTTACC TGTGCTCCGC CGTGCTTCCT
    CTCATCACTA AATGTGCTCC TTTGTTCGCC GGGACGGACC ACCGAGCCAT CATGATCGAC
    TCCATGCTTC ACACCATCTA CAGGCTGTCT CGAGGACGCT CGCTAACTAA AGCACAAAGA
    GATGTTATTG AGGAATGCCT CATGTCTTTG TGCAGGTATC TGCGCCCTTC CATGCTCCAG
    CACCTTCTCA GGCGGCTAGT ATTTGATGTG CCTATTCTGA ATGAGTATGC CAAAATGCCC
    CTTAAGCTGC TTACCAATCA TTATGAGCGT TGTTGGAAAT ATTATTGCTT GCCTAATGGC
    TGGGCTAACT TTGGCGTATC ATCAGAAGAG GAGCTTCATC TGACCCGTAA ACTCTTTTGG
    GGGATCTTTG AATCTCTGGC TCACAAGAAA TTCGATGCAG AGCTGTTCAA AATCGCCATG
    CCTTGTATAT GTGCGATCGC TGGTGCAATT CCTCCTGATT ATGTGGACGC CAGCTACTCC
    TCCAAAACCG AAAAAAAGGC TTCTGTTGAT GCTGAAGGGA ACTTTGACCC CAAACCTGTT
    GATACCACTA ACACTATAAT TCCCGAGAAG TTGGACGGGT TCATCAACAG ATATGCAGAA
    TATACGCACG ACAGATGGGC TTTTGAAAAA ATTCAAAACA ACTGGACATT TGGTGAGGTG
    ATGGATGAAA ATGCCAAGAC TCATCCCATG CTCAGACCTT ACAAAACATT CTCTGAAAAG
    GATAAAGAAA TCTACCGCTG GCCTATTAAA GAGTCCATCA AGGCCATGAT TGCCTGGGAG
    TGGACACTGG ACAAAGCCAG AGATGGAGAG GATGAGAAAG TTGAGAAGAA AACAGCCCGT
    AAGATCTCAC AGACTGCACA GGCAACCTAT GACCCCAGTC ATGGCTATAG CCCCCAACCT
    ATTGATATCT CAGGCATGAC ACTGTCCAGA GAGCTACAGT CCATGGCAGA GCAGCTCGCA
    GAAAACTACC ACAACACCTG GGGCCGTAAG AAGAAAATGG AATTACAAGC AAAAGGAGGA
    GGTACACACC CTTTGCTTGT GCCTTATGAC ACACTGACGG CTAAAGAAAA GGCACGAGAT
    AGAGAGAAAG CGTATGAGCT ACTCAAGTTT CTGCAGCTCA ATGGATATGC TGTGACAAGA
    GGGCTCAAAG ACATGGAAAG TGACATTTCA TCTATCGAGA AGCGTTTTGC GTATGGCTTC
    CTACAGAAGC TTCTGAAGTG GATGGACATT GCTCAGGAGT TCATAGCACA TCTTGAGGCG
    GTTGTGAGCA GCGGTCGTGT GGAAAAGTCA CCACATGAGC AAGAAATCAA GTTCTTTGCC
    AAGATTCTTT TGCCTTTGAT AAATCAGTAT TTTAAAAACC ACTGCCTTTA CTTCCTCTCC
    ACACCGGCCA AAATCCTAGG CAGCGGCGGA CACGCCTCAA ACAAGGAAAA GGAGATGATT
    GCAAGCATTT TCTGTAAGAT GTCTGCCCTG GTGCGACACA GAGTGTTTCT CTTTGGCACG
    GATGCGCCTG CTATTGTCAA CTGCCTTCAT ATCCTCGCAC GTTCGCTTGA TGCCAGGACG
    GTGATGAAAT CCGGGCCTGA GATTGTGAAA GCAGGACTGC GTTTGTTCTT TGAGAGTGCA
    GCTGATGACA TTGAAAAGAT GGTGGAGAAT CTCAAACTTG GCAAAGTGTC CAAAGGCAAT
    CAGCCAGTGA AAGGTGTGTC CCAGAATATA AACTACACCA CAACGGCTTT GCTTCCTGTC
    CTCACATCAC TGTTTGACCA CATCGCTCAA CACCAATTCG GAGATGATGT TATCCTGGAT
    GATTTGCAGA TGTCCTGCTA CCGAATCATG TGTAGCATCT ACTCTCTTGG GACTGTAAAA
    ACTCCACATG CAGAGAGGCA TAGACCAGCC CTTGGAGAAT GTCTCGCTCA TCTCGCTGCA
    GCAATGCCTG TTGCCTACCT GGAGCCGCAT CTTAATGAAT ACAATGCCTT TTCAGTCTAC
    ACTACCAAGA CACCGAGAGA AAGAGCTATC CTTGGCCTTC CAAACCAAGT TCAAGAGCTG
    TGTTTGGACA TTCCTGAGCT TGACGTTTTA CTTAAGGAGA TCGGAGACCT GGCTGAATCG
    GGAGCTCGAT ACACTGAAAT GCCACATGTG ATTGAGGTCA CTCTGCCCAT GCTGTGTAAC
    TATCTGCCCC GCTGGTGGGA GAGAGGACTG GAGAACTTCC CGGATCTGGA GGGTAAGCTG
    TGCACCGACG TCACCTCTGA ATCCCTCAAC CAGCTTTTGG GAAGCATCAT GAAAATCGTG
    GTCAACAACT TGGGAATCGA CGAAGCCTCC TGGATGAAGA GACTTGCAGT GTTCTCCCAA
    CCCATTGTGA GCCGAGCCAA ACCAGAGATG CTCAAATCCC ACTTCATCCC CACCATGGAG
    AAGCTGAAGA AGCGAACGTC TAAAGTTGTA GCCGAGGAGG AGCATCTGCG TTTGGAGGGA
    AAGTCTGAGG GAGATGAGGA AGAGGGAACC ATCAGAGACG AGTTTGCAGT TCTCTGTAGA
    GACCTCTATG CTCTATATCC TCTTCTCATC CGCTATGTGG ATAACAACAG AGCAAGATGG
    CTGACGTTTC GAGACCCAGA TGCTGAGGAG TTGTTCCGCA TGGTGGGAGA GGTCTTCATC
    TTCTGGTCAA AATCTCACAA TTTCAAGCGA GAGGAGCAAA ACTTTGTGGT GATGAACGAA
    ATCAACAATA TGTCCTTTCT CACTGCTGAC AGTAAGAGCA AGATGAGCAA GGCTGGAGGG
    TCGGATGTGG AGCGTACCAA GAAAAAGAGA CGGGGGGATC GATACTCTGT GCAAACGTCC
    CTCATTGTGG CCGCACTGAA GAAAATGCTT CCCATTGGAC TTAACATGTG TTCTCCTGCA
    GACCAGGAGC TGATTAATCT GGCAAAGACA CGTTATTCTC TGAAAGACAC AGATGAGGAG
    GTCAGAGAGT TCTTGCAAAA TAACCTGCAC CTTCAGGGAA AGGTTGATAA CCCATCTATG
    AGGTGGCAGA TGGCACTGTA TAAGGAAATG GCAGGAAAGG CTGAAGATGC AGATGCCCCA
    GAGAATGTGG TCAAGAGGGT TCAGGAGGTC TCTGCGGTCC TTTACCACAT TGAAGTGACG
    GAGCACCCTT TCAAGTCCAA AAAGATGGTT TGGCACAAAC TGCTGTCGAA GCAGCGGCGT
    AGAGCGGTGG TGGCCTGTTT CAGGATGACT CCACTTTACA ACTTGCCCAG ACACAGAGCA
    TCTAATATGT TCCTGGATGG GTACAAGCGC AACTGGATAA GAAATGAAGG CTATTCCTTT
    GAGGACAGAA TGATTGATGA CCTGTCTAAA GCATTGGAGG AGGAAGAAGA GGAGGAAGAG
    GAAAAGGAGA CAAAGCCAGA CCCTCTTCAT CAGCTCATCC TTCACTTTAG TCGCACAGCA
    CTCACTGAGA AAAGTAAACT GGATATAGAT CACCTATACA TGGCATATGC TGATATCATG
    GCTAAGAGTT GCCACATGGA TGAAGAGGAT GAGGGTGGAG AGGAGGAGAA TGAGGGTGGA
    GAGGAGGAGA TGTCCTTTGA GGAAAAGGAA ATGGAAAAGC AAAGACTGCT CTACCAGCAG
    TCGCGTCTAC ACAACCGAGG GGCTGCTGAA ATGGTCCTGC AGATGATCAG TGCCTGCAAG
    GGTGAGACTG GTTGTATGGT CTCTTCCACC CTAAAACTTG GCATTTCTAT TTTAAATGGC
    GGGAACTGTG AAGTTCAGCA GAAAATGCTG GACTATCTGA AAGACAAGAA AGATGTGGGT
    TTCTTTCTCA GTGTTCAGGC TTTAATGCAG ACCTGCAGTG TTTTGGACCT GAATGCCTTT
    GAGAGGCAGA ATAAAGCTGA GGGTCTAGGT ATGGTGTCAG AAGAGGGTAC AAACATGAAG
    CTAGAACGGG GTGAGAAGGT CATGGCTGAT GACGAGTTCA CTTGCGACCT CTTCCGCTTT
    CTCCAGCTCC TCTGTGAGGG TCACAACAAT GACTTCCAAA ATTACCTCAG AACACAGACA
    GGCAGCACCA CCACCATCAA TGTCATTATC TGTACAGTGG ATTACCTGCT TAGACTACAG
    GAGTCCATCA GTGATTTCTA CTGGTATTAC TCGGGAAAAG ACATCATAGA TGAACCAGGA
    AAGCGAAACT TCTCTAAAGC TATGACTGTG GCCAAACAGG TTTTCAACAG TCTTACCGAG
    TATATCCAGG GTCCCTGCAC AGGAAACCAG CAGTCCCTGG CTCACAGTCG CTTATGGGAT
    GCTGTGGTGG GCTTTCTGCA CGTGTTTGCT CATATGATGA TGAAACTGGC TCAGGATTCC
    AGTCAGATTG GTCTTCTGAA AGAGTTGTTG GACCTTCAGA AAGACATGGT GGTCATGTTA
    TTGTCCTTGT TGGAGGGTAA TGTTGTAAAT GGCACCATTG CTCGCCAGAT GGTGGACATG
    CTAGTGGAAT CCTCCAGCAA TGTAGAAATG ATCCTTAAGT TCTTCGACAT GTTCCTCAAG
    CTGAAGGACA TTGTTGCGTC TGACGCGTTT CGTGACTACG TGACCGACCC TAGAGGTTTG
    ATATCCAAGA AAGACTTTCA GAAATCCATG GACAGCCAGA AACAGTACAC ACCTTCAGAG
    ATCCAATTCC TATTGTCATG CTCTGAGGCT GATGAGAATG AGATGATCAA TTACGAGGAG
    TTTGCAAGTC GCTTTCAAGA GCCGGCCAAA GATATTGGTT TTAACATAGC GGTGCTGCTC
    ACCAACCTCT CCGAACATGT TCCTCACGAT ACAAGACTGC AGAACTTCCT CGAACAAGCC
    GAGAGCGTCC TGAACTATTT CCGCCCATTC TTGGGTCGCA TCGAGATCAT GGGCGCCAGC
    AGGAAAATCG AGAGAATCTA CTTCGAGATC AGCGAGGTGA ATCGCAAGCA GTGGGAAATG
    CCTCAAGTCA AAGAATCGAA GAGACAGTTC ATATTCGACG TAGTCAATGA AGGCGGCGAA
    TCGGAGAAGA TGGAGCTTTT CGTCAACTTC TGCGAGGACA CCATATTCGA GATGAACATC
    GCGTCGCAGA TTTCAGAACA AGACGAGGAA GAGAAAGAAG AGGATGATAT TGAGGAAGCT
    GAAGGAGGAG ATGCTGGTGA TGAAGAGGGG GGTGGCGAGG AGGGACAACC AGAATCAAGC
    TCTGCATTTG CCGATTTCAT TCAAAGCATC ATGAATTTTC TGGGTATGTT CACCTTCAGG
    AATATTCGCA GACAGTACCG CAGACTCCGC AAGATGACCA TTAAGGAGAT CGTGGTAGGG
    CTTGCTACCT TCATGTGGAC GATTCTCTTG GGCATCCTCC ATTTCATTTA CAGTGTTTGT
    AAAGGCTTCT TCCTCCTCAT CTGGCACACG CTGTTTGGAG GAGGTTTGGT TGAAGGGGCC
    AAAAACATGA CAGTAACTGA GTTACTAGCC AGCATGCCAG ATCCCACACA GGACGAGGTG
    CATGGGGACT TACCTGGAGA ACCGAGGGGA GGAGAGGAAC AGGAGACTGG AGGGATAATG
    GACTCGATGG ATGCTGGAGG GGGAGAGGAA GAGGATGAGG ATATCCAGGA GAAAGATGGT
    GGAAAGATTC CTGGTATAGA TACACCTGGT GGACTTGGAG ATATGGGTGA TACAACTCCT
    GTAGAACCGC CGACTCCCGA GGGTACCCCT TTGTTGAAGA GGAAAGCGCA ACCAGATGAA
    GCAGGACCTG AACAGCCTCC TGCTATTGAA GAGCCTCCTC CTGAGCCTGA GAAAGCAGAC
    ATTGAGAATG GTGAGAAGGC TGAGAAGGAA GTGGAGGTCA AGCCTGAGCC GATACCAGAA
    GAACCGAAAC CTCAGAGAGC CACCAAAGCC AAGAAGGAGA AGAAATCAGT CAAGGGTGCA
    GGATTTGAGC TCTGGAATGA GCTGGATGTG CAAAGAAATA AATTCATGAA CTGCTTATCT
    CGTAACTTCT ACAACATTCG TTTCCTGGCT CTGTTCCTTG CCTTTGCCTT GAACTTCATT
    CTTCTCTTCT ACAAGGTATC TGATACTCCC CCCAGTTCCG ATGAATTTGA AGGTTCTGGT
    ATCTTTGAAG GCTCTGGGCT GTTTGAAGGC TCTGCGGAGG ATTTCGAGGG CTCTGGTGGA
    GAGGATGGAG GTGATGATGA GGATGAAGAA GGACCTGTGT ATTATTTTCT GGAAGAAAGC
    ACGGGATACA TGCAGCCCAC TCTGTCCTTC CTGGCCATTT TGCACACCGT CATTGCTTTC
    ATATCTATCA TTGGATACAA CTGCCTCAAG ATTCCACTGG TCATCTTTAA GAGAGAGAAA
    GAGTTGGCGA GAAAACTGGA GTTTGATGGT CTTTACATCA CTGAACAGCC GGCAGATGAT
    GACATTAAAG GCCAGTGGGA CAGACTGGTG TTAAACACTC CCTCTTTTCC CACCAATTAC
    TGGGACAAAT TTGTGAAGCG AAAGGTTCTG GATAAATATG GAGATATTTA TGGACGAGAG
    AGAATCGCAG AGCTGCTGGG TATGGATCTG GCATCTCTGG ATGTCAGCAA ACAACAGACA
    GACAAGAAAC CAGAAGAGCC AGACAACTCT ATGCTTGCAT GGTTCACTTC CATTGACATC
    AAGTATCAGA TCTGGAAATT TGGAGTTGTG TTCACAGACA ATACTTTCCT CTACCTGGTG
    TGGTACACAG TGATGTCCCT TCTTGGACAT TACAACAACT TTTTCTTTGC TTGTCACCTG
    CTGGACATCG CAATGGGTGT CAAGACACTG CGTACAATTC TTTCCTCCGT CACACACAAT
    GGAAAACAGC TGATGATGAC TGTGGGTTTG CTGGCTGTGG TTGTGTACCT CTACACTGTG
    GTGGCCTTCA ACTTCTTCAG GAAGTTCTAC AATAAGAGCG AGGATGAGGA TGAGCCGGAC
    ATGAAGTGTG ACGACATGAT GACGTGTTAT TTGTTCCACA TGTATGTGGG TGTACGGGCA
    GGTGGAGGTA TAGGAGATGA GATCGAGGAC CCTGCTGGAG ATGAGTATGA GCTCTATCGG
    GTGGTCTTCG ACATCACCTT CTTCTTCTTT GTCATCGTCA TCCTGCTGGC CATCATTCAG
    GGTCTGATCA TTGATGCCTT TGGTGAACTG AGAGACCAGC AGGAGCAAGT GAAAGAGGAC
    ATGGAGACCA AATGCTTTAT ATGTGGCATT GGGAGCGACT ACTTTGATAC AACGCCCCAT
    GGCTTTGAGA CCCATACACT TGAGGAACAC AACCTGGCCA ATTACATGTT TTTCCTGATG
    TACTTAATCA ACAAGGATGA AACAGAGCAC ACTGGCCAGG AATCATACGT GTGGAAGATG
    TACCAGGAGA GATGCTGGGA TTTCTTCCCT GCCGGAGACT GCTTCAGAAA GCAATATGAA
    GACCAGCTTG GATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021327995.1
    CDS188..8962
    Translation

    Target ORF information:

    RefSeq Version XM_021472320.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio ryanodine receptor 1a (skeletal) (ryr1a), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021472320.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    ATGATCTACT GGGCTCAAGA GTCTTTCATC CAGAACCCTG AGCTGGTGCG CCTCATGTTT 
    AGTCTGCTAC ACCGGCAGTA TGACGCTTTG GGTGAGCTGA TTCGAGCTTT GCCCAAAGCT
    TATATCATTA ATGCTGTTTC TATACAAGAC ACAATGGAGC TCCTGGAGTG TCTGGGACAG
    ATACGCTCCT TGCTCATCGT TCAGATGGGA CCAGAGGAAG AGAGGCTGAT GATCCAGAGT
    ATCGGAAATA TTATGAGTAA CAGGGTATTT TACCAGCACC CCAACTTGAT GAGAGCTCTG
    GGAATGCATG AGACAGTCAT GGAGGTTATG GTGAACGTAC TCGGAGGCGG AGATTCCAAG
    GAGATCAGAT TCCCTCGAAT GGTCACCAAC TGCTGTCGTT TCCTGTGTTA TTTCTGTCGT
    ATCAGCAGAC AGAATCAGCG CTCGATGTTT GATCATCTGG GCTACCTCCT GCAGAACAGT
    GGCATTGGGC TGGGAATGCG TGGCTCCACT CCTCTTGATG TTGCTGCTGC TTCCTGTATC
    GATAATAATG AGCTTGCCCT GGCTCTCCAA GAGCAAGACC TGGAGATGGT AGTAAAGTAC
    TTGGCAGGCT GTGGTCTGCA GAGCTGCCCT ATGCTCTTGT CAAAGGGTTA CCCGGATATT
    GGATGGAACC CAGTTGGAGG AGAAAAGTAT CTGGATTTCC TGCGCTTTGC TGTGTTTGTG
    AATGGAGAGA GTGTGGAGGA GAACGCTAAT GTGGTGGTCC GGCTGCTGAT CAGGAGGCCT
    GAGTGCTTTG GTCCTGCACT GAGAGGAGAA GGGGGCAATG GTCTGCTGGC TGCCATTGAA
    GAAGCCATCA AGATCTCAGA GGACCCTGCA AGAGATGGAC CCACTGTTAA AAAAGACAGA
    CGCTTCCCGA TGTTTGGAGG TGAAGAGCAG CATGAGGAGA ACAGAGTGCA CCTAGGAAAT
    GCCATCATGT CATTTTACTC AGCTCTTATT GATCTGCTGG GTCGCTGTGC TCCTGAGATG
    CATTTGATCC AGGCTGGTAA AGGTGAAGCT TTGAGAATCA GGGCTATTCT TCGGTCGCTT
    GTGCCCATTG AGGATCTGGT CGGTGTGATC AGCTTGTCAG TCCAGATTCC TGCTTTTGGA
    AAAGATAACA GCATTATTGA GCCGAAGATG TCTGCTAGTT TTGTTCCTGA TCATAAAGCA
    CCAATGGTCC TGTTCCTGGA CAGAGTTTAT GGCATCGACA ATCAGGACTT CCTATTGCAT
    GTACTAGAAG TGGGTTTCCT GCCTGATATG AGGGCGGCAG CCTCCTTAGA CACAGCTGCA
    TTCAGCACTA CAGAAATGGC CTTGGCTCTG AACCGTTACC TGTGCTCCGC CGTGCTTCCT
    CTCATCACTA AATGTGCTCC TTTGTTCGCC GGGACGGACC ACCGAGCCAT CATGATCGAC
    TCCATGCTTC ACACCATCTA CAGGCTGTCT CGAGGACGCT CGCTAACTAA AGCACAAAGA
    GATGTTATTG AGGAATGCCT CATGTCTTTG TGCAGGTATC TGCGCCCTTC CATGCTCCAG
    CACCTTCTCA GGCGGCTAGT ATTTGATGTG CCTATTCTGA ATGAGTATGC CAAAATGCCC
    CTTAAGCTGC TTACCAATCA TTATGAGCGT TGTTGGAAAT ATTATTGCTT GCCTAATGGC
    TGGGCTAACT TTGGCGTATC ATCAGAAGAG GAGCTTCATC TGACCCGTAA ACTCTTTTGG
    GGGATCTTTG AATCTCTGGC TCACAAGAAA TTCGATGCAG AGCTGTTCAA AATCGCCATG
    CCTTGTATAT GTGCGATCGC TGGTGCAATT CCTCCTGATT ATGTGGACGC CAGCTACTCC
    TCCAAAACCG AAAAAAAGGC TTCTGTTGAT GCTGAAGGGA ACTTTGACCC CAAACCTGTT
    GATACCACTA ACACTATAAT TCCCGAGAAG TTGGACGGGT TCATCAACAG ATATGCAGAA
    TATACGCACG ACAGATGGGC TTTTGAAAAA ATTCAAAACA ACTGGACATT TGGTGAGGTG
    ATGGATGAAA ATGCCAAGAC TCATCCCATG CTCAGACCTT ACAAAACATT CTCTGAAAAG
    GATAAAGAAA TCTACCGCTG GCCTATTAAA GAGTCCATCA AGGCCATGAT TGCCTGGGAG
    TGGACACTGG ACAAAGCCAG AGATGGAGAG GATGAGAAAG TTGAGAAGAA AACAGCCCGT
    AAGATCTCAC AGACTGCACA GGCAACCTAT GACCCCAGTC ATGGCTATAG CCCCCAACCT
    ATTGATATCT CAGGCATGAC ACTGTCCAGA GAGCTACAGT CCATGGCAGA GCAGCTCGCA
    GAAAACTACC ACAACACCTG GGGCCGTAAG AAGAAAATGG AATTACAAGC AAAAGGAGGA
    GGTACACACC CTTTGCTTGT GCCTTATGAC ACACTGACGG CTAAAGAAAA GGCACGAGAT
    AGAGAGAAAG CGTATGAGCT ACTCAAGTTT CTGCAGCTCA ATGGATATGC TGTGACAAGA
    GGGCTCAAAG ACATGGAAAG TGACATTTCA TCTATCGAGA AGCGTTTTGC GTATGGCTTC
    CTACAGAAGC TTCTGAAGTG GATGGACATT GCTCAGGAGT TCATAGCACA TCTTGAGGCG
    GTTGTGAGCA GCGGTCGTGT GGAAAAGTCA CCACATGAGC AAGAAATCAA GTTCTTTGCC
    AAGATTCTTT TGCCTTTGAT AAATCAGTAT TTTAAAAACC ACTGCCTTTA CTTCCTCTCC
    ACACCGGCCA AAATCCTAGG CAGCGGCGGA CACGCCTCAA ACAAGGAAAA GGAGATGATT
    GCAAGCATTT TCTGTAAGAT GTCTGCCCTG GTGCGACACA GAGTGTTTCT CTTTGGCACG
    GATGCGCCTG CTATTGTCAA CTGCCTTCAT ATCCTCGCAC GTTCGCTTGA TGCCAGGACG
    GTGATGAAAT CCGGGCCTGA GATTGTGAAA GCAGGACTGC GTTTGTTCTT TGAGAGTGCA
    GCTGATGACA TTGAAAAGAT GGTGGAGAAT CTCAAACTTG GCAAAGTGTC CAAAGGCAAT
    CAGCCAGTGA AAGGTGTGTC CCAGAATATA AACTACACCA CAACGGCTTT GCTTCCTGTC
    CTCACATCAC TGTTTGACCA CATCGCTCAA CACCAATTCG GAGATGATGT TATCCTGGAT
    GATTTGCAGA TGTCCTGCTA CCGAATCATG TGTAGCATCT ACTCTCTTGG GACTGTAAAA
    ACTCCACATG CAGAGAGGCA TAGACCAGCC CTTGGAGAAT GTCTCGCTCA TCTCGCTGCA
    GCAATGCCTG TTGCCTACCT GGAGCCGCAT CTTAATGAAT ACAATGCCTT TTCAGTCTAC
    ACTACCAAGA CACCGAGAGA AAGAGCTATC CTTGGCCTTC CAAACCAAGT TCAAGAGCTG
    TGTTTGGACA TTCCTGAGCT TGACGTTTTA CTTAAGGAGA TCGGAGACCT GGCTGAATCG
    GGAGCTCGAT ACACTGAAAT GCCACATGTG ATTGAGGTCA CTCTGCCCAT GCTGTGTAAC
    TATCTGCCCC GCTGGTGGGA GAGAGGACTG GAGAACTTCC CGGATCTGGA GGGTAAGCTG
    TGCACCGACG TCACCTCTGA ATCCCTCAAC CAGCTTTTGG GAAGCATCAT GAAAATCGTG
    GTCAACAACT TGGGAATCGA CGAAGCCTCC TGGATGAAGA GACTTGCAGT GTTCTCCCAA
    CCCATTGTGA GCCGAGCCAA ACCAGAGATG CTCAAATCCC ACTTCATCCC CACCATGGAG
    AAGCTGAAGA AGCGAACGTC TAAAGTTGTA GCCGAGGAGG AGCATCTGCG TTTGGAGGGA
    AAGTCTGAGG GAGATGAGGA AGAGGGAACC ATCAGAGACG AGTTTGCAGT TCTCTGTAGA
    GACCTCTATG CTCTATATCC TCTTCTCATC CGCTATGTGG ATAACAACAG AGCAAGATGG
    CTGACGTTTC GAGACCCAGA TGCTGAGGAG TTGTTCCGCA TGGTGGGAGA GGTCTTCATC
    TTCTGGTCAA AATCTCACAA TTTCAAGCGA GAGGAGCAAA ACTTTGTGGT GATGAACGAA
    ATCAACAATA TGTCCTTTCT CACTGCTGAC AGTAAGAGCA AGATGAGCAA GGCTGGAGGG
    TCGGATGTGG AGCGTACCAA GAAAAAGAGA CGGGGGGATC GATACTCTGT GCAAACGTCC
    CTCATTGTGG CCGCACTGAA GAAAATGCTT CCCATTGGAC TTAACATGTG TTCTCCTGCA
    GACCAGGAGC TGATTAATCT GGCAAAGACA CGTTATTCTC TGAAAGACAC AGATGAGGAG
    GTCAGAGAGT TCTTGCAAAA TAACCTGCAC CTTCAGGGAA AGGTTGATAA CCCATCTATG
    AGGTGGCAGA TGGCACTGTA TAAGGAAATG GCAGGAAAGG CTGAAGATGC AGATGCCCCA
    GAGAATGTGG TCAAGAGGGT TCAGGAGGTC TCTGCGGTCC TTTACCACAT TGAAGTGACG
    GAGCACCCTT TCAAGTCCAA AAAGATGGTT TGGCACAAAC TGCTGTCGAA GCAGCGGCGT
    AGAGCGGTGG TGGCCTGTTT CAGGATGACT CCACTTTACA ACTTGCCCAG ACACAGAGCA
    TCTAATATGT TCCTGGATGG GTACAAGCGC AACTGGATAA GAAATGAAGG CTATTCCTTT
    GAGGACAGAA TGATTGATGA CCTGTCTAAA GCATTGGAGG AGGAAGAAGA GGAGGAAGAG
    GAAAAGGAGA CAAAGCCAGA CCCTCTTCAT CAGCTCATCC TTCACTTTAG TCGCACAGCA
    CTCACTGAGA AAAGTAAACT GGATATAGAT CACCTATACA TGGCATATGC TGATATCATG
    GCTAAGAGTT GCCACATGGA TGAAGAGGAT GAGGGTGGAG AGGAGGAGAA TGAGGGTGGA
    GAGGAGGAGA TGTCCTTTGA GGAAAAGGAA ATGGAAAAGC AAAGACTGCT CTACCAGCAG
    TCGCGTCTAC ACAACCGAGG GGCTGCTGAA ATGGTCCTGC AGATGATCAG TGCCTGCAAG
    GGTGAGACTG GTTGTATGGT CTCTTCCACC CTAAAACTTG GCATTTCTAT TTTAAATGGC
    GGGAACTGTG AAGTTCAGCA GAAAATGCTG GACTATCTGA AAGACAAGAA AGATGTGGGT
    TTCTTTCTCA GTGTTCAGGC TTTAATGCAG ACCTGCAGTG TTTTGGACCT GAATGCCTTT
    GAGAGGCAGA ATAAAGCTGA GGGTCTAGGT ATGGTGTCAG AAGAGGGTAC AAACATGAAG
    CTAGAACGGG GTGAGAAGGT CATGGCTGAT GACGAGTTCA CTTGCGACCT CTTCCGCTTT
    CTCCAGCTCC TCTGTGAGGG TCACAACAAT GACTTCCAAA ATTACCTCAG AACACAGACA
    GGCAGCACCA CCACCATCAA TGTCATTATC TGTACAGTGG ATTACCTGCT TAGACTACAG
    GAGTCCATCA GTGATTTCTA CTGGTATTAC TCGGGAAAAG ACATCATAGA TGAACCAGGA
    AAGCGAAACT TCTCTAAAGC TATGACTGTG GCCAAACAGG TTTTCAACAG TCTTACCGAG
    TATATCCAGG GTCCCTGCAC AGGAAACCAG CAGTCCCTGG CTCACAGTCG CTTATGGGAT
    GCTGTGGTGG GCTTTCTGCA CGTGTTTGCT CATATGATGA TGAAACTGGC TCAGGATTCC
    AGTCAGATTG GTCTTCTGAA AGAGTTGTTG GACCTTCAGA AAGACATGGT GGTCATGTTA
    TTGTCCTTGT TGGAGGGTAA TGTTGTAAAT GGCACCATTG CTCGCCAGAT GGTGGACATG
    CTAGTGGAAT CCTCCAGCAA TGTAGAAATG ATCCTTAAGT TCTTCGACAT GTTCCTCAAG
    CTGAAGGACA TTGTTGCGTC TGACGCGTTT CGTGACTACG TGACCGACCC TAGAGGTTTG
    ATATCCAAGA AAGACTTTCA GAAATCCATG GACAGCCAGA AACAGTACAC ACCTTCAGAG
    ATCCAATTCC TATTGTCATG CTCTGAGGCT GATGAGAATG AGATGATCAA TTACGAGGAG
    TTTGCAAGTC GCTTTCAAGA GCCGGCCAAA GATATTGGTT TTAACATAGC GGTGCTGCTC
    ACCAACCTCT CCGAACATGT TCCTCACGAT ACAAGACTGC AGAACTTCCT CGAACAAGCC
    GAGAGCGTCC TGAACTATTT CCGCCCATTC TTGGGTCGCA TCGAGATCAT GGGCGCCAGC
    AGGAAAATCG AGAGAATCTA CTTCGAGATC AGCGAGGTGA ATCGCAAGCA GTGGGAAATG
    CCTCAAGTCA AAGAATCGAA GAGACAGTTC ATATTCGACG TAGTCAATGA AGGCGGCGAA
    TCGGAGAAGA TGGAGCTTTT CGTCAACTTC TGCGAGGACA CCATATTCGA GATGAACATC
    GCGTCGCAGA TTTCAGAACA AGACGAGGAA GAGAAAGAAG AGGATGATAT TGAGGAAGCT
    GAAGGAGGAG ATGCTGGTGA TGAAGAGGGG GGTGGCGAGG AGGGACAACC AGAATCAAGC
    TCTGCATTTG CCGATTTCAT TCAAAGCATC ATGAATTTTC TGGGTATGTT CACCTTCAGG
    AATATTCGCA GACAGTACCG CAGACTCCGC AAGATGACCA TTAAGGAGAT CGTGGTAGGG
    CTTGCTACCT TCATGTGGAC GATTCTCTTG GGCATCCTCC ATTTCATTTA CAGTGTTTGT
    AAAGGCTTCT TCCTCCTCAT CTGGCACACG CTGTTTGGAG GAGGTTTGGT TGAAGGGGCC
    AAAAACATGA CAGTAACTGA GTTACTAGCC AGCATGCCAG ATCCCACACA GGACGAGGTG
    CATGGGGACT TACCTGGAGA ACCGAGGGGA GGAGAGGAAC AGGAGACTGG AGGGATAATG
    GACTCGATGG ATGCTGGAGG GGGAGAGGAA GAGGATGAGG ATATCCAGGA GAAAGATGGT
    GGAAAGATTC CTGGTATAGA TACACCTGGT GGACTTGGAG ATATGGGTGA TACAACTCCT
    GTAGAACCGC CGACTCCCGA GGGTACCCCT TTGTTGAAGA GGAAAGCGCA ACCAGATGAA
    GCAGGACCTG AACAGCCTCC TGCTATTGAA GAGCCTCCTC CTGAGCCTGA GAAAGCAGAC
    ATTGAGAATG GTGAGAAGGC TGAGAAGGAA GTGGAGGTCA AGCCTGAGCC GATACCAGAA
    GAACCGAAAC CTCAGAGAGC CACCAAAGCC AAGAAGGAGA AGAAATCAGT CAAGGGTGCA
    GGATTTGAGC TCTGGAATGA GCTGGATGTG CAAAGAAATA AATTCATGAA CTGCTTATCT
    CGTAACTTCT ACAACATTCG TTTCCTGGCT CTGTTCCTTG CCTTTGCCTT GAACTTCATT
    CTTCTCTTCT ACAAGGTATC TGATACTCCC CCCAGTTCCG ATGAATTTGA AGGTTCTGGT
    ATCTTTGAAG GCTCTGGGCT GTTTGAAGGC TCTGCGGAGG ATTTCGAGGG CTCTGGTGGA
    GAGGATGGAG GTGATGATGA GGATGAAGAA GGACCTGTGT ATTATTTTCT GGAAGAAAGC
    ACGGGATACA TGCAGCCCAC TCTGTCCTTC CTGGCCATTT TGCACACCGT CATTGCTTTC
    ATATCTATCA TTGGATACAA CTGCCTCAAG ATTCCACTGG TCATCTTTAA GAGAGAGAAA
    GAGTTGGCGA GAAAACTGGA GTTTGATGGT CTTTACATCA CTGAACAGCC GGCAGATGAT
    GACATTAAAG GCCAGTGGGA CAGACTGGTG TTAAACACTC CCTCTTTTCC CACCAATTAC
    TGGGACAAAT TTGTGAAGCG AAAGGTTCTG GATAAATATG GAGATATTTA TGGACGAGAG
    AGAATCGCAG AGCTGCTGGG TATGGATCTG GCATCTCTGG ATGTCAGCAA ACAACAGACA
    GACAAGAAAC CAGAAGAGCC AGACAACTCT ATGCTTGCAT GGTTCACTTC CATTGACATC
    AAGTATCAGA TCTGGAAATT TGGAGTTGTG TTCACAGACA ATACTTTCCT CTACCTGGTG
    TGGTACACAG TGATGTCCCT TCTTGGACAT TACAACAACT TTTTCTTTGC TTGTCACCTG
    CTGGACATCG CAATGGGTGT CAAGACACTG CGTACAATTC TTTCCTCCGT CACACACAAT
    GGAAAACAGC TGATGATGAC TGTGGGTTTG CTGGCTGTGG TTGTGTACCT CTACACTGTG
    GTGGCCTTCA ACTTCTTCAG GAAGTTCTAC AATAAGAGCG AGGATGAGGA TGAGCCGGAC
    ATGAAGTGTG ACGACATGAT GACGTGTTAT TTGTTCCACA TGTATGTGGG TGTACGGGCA
    GGTGGAGGTA TAGGAGATGA GATCGAGGAC CCTGCTGGAG ATGAGTATGA GCTCTATCGG
    GTGGTCTTCG ACATCACCTT CTTCTTCTTT GTCATCGTCA TCCTGCTGGC CATCATTCAG
    GGTCTGATCA TTGATGCCTT TGGTGAACTG AGAGACCAGC AGGAGCAAGT GAAAGAGGAC
    ATGGAGACCA AATGCTTTAT ATGTGGCATT GGGAGCGACT ACTTTGATAC AACGCCCCAT
    GGCTTTGAGA CCCATACACT TGAGGAACAC AACCTGGCCA ATTACATGTT TTTCCTGATG
    TACTTAATCA ACAAGGATGA AACAGAGCAC ACTGGCCAGG AATCATACGT GTGGAAGATG
    TACCAGGAGA GATGCTGGGA TTTCTTCCCT GCCGGAGACT GCTTCAGAAA GCAATATGAA
    GACCAGCTTG GATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa147734
    Clone ID Related Accession (Same CDS sequence) XM_021479405.1
    Accession Version XM_021479405.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 15210bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product ryanodine receptor 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
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    3601
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    4141
    4201
    4261
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    9301
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    9421
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    9781
    9841
    9901
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    10021
    10081
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    10261
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    10501
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    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    13261
    13321
    13381
    13441
    13501
    13561
    13621
    13681
    13741
    13801
    13861
    13921
    13981
    14041
    14101
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    14221
    14281
    14341
    14401
    14461
    14521
    14581
    14641
    14701
    14761
    14821
    14881
    14941
    15001
    15061
    15121
    15181
    ATGGCAGAAG GAGCTGAGGG AGAAGAGGAG ATCCAATTCC TAAGAACAGA TGACCAGGTG 
    GTGCTACAGT GTACAGCTAC TATCCTGAAG GAGCAGATTA AACTCTGTCT GTCCTGTGAG
    GGTTTTGGGA ATCGCCTGTG TTTTTTAGAA ACCACATCTA ATGCTCAGAA TGTTCCTCCT
    GATCTGGCAA TATGCTGCTT TATCCTGGAG CAGTCTTTAT CTGTGCGGGC CCTGCAGGAG
    ATGCTGGCTA ACACTACTGA GCTTAATGAG GCAGTCGATT TGGATAAATG GTCTTCACAA
    GGAGGAGGCC ACCGCACGTT GTTGTATGGC CACGCTATCC TACTCAGACA TACTCACTCT
    GGCATGTACC TTAGTTGCTT GACCACATCA AGATCCCTCA CAGACAAACT GGCCTTTGAT
    GTGGGCTTGC AGGAGGACTC CACAGGTGAG GCCTGTTGGT GGACCATTCA CCCTGCATCC
    AAGCAGAGGT CAGAGGGTGA GAAGGTCAGA GTGGGCGATG ACATTATTCT GGTCAGCGTG
    TCCTCTGAGA GATATCTGCA CCTCTCCTAT GCCAGCGGAG ACCTGATGGT GGACGCTTCT
    TTCATGCAGA CCCTTTGGAA CATGAACCCA GTCTGTTCAG GCTGTGAGCT GGCAGAGGGG
    TTTCTGACTG GAGGGCATGT TCTTCGTCTA TTTCACGGGC ACATGGATGA GTGTTTAACA
    ATTCCTGGCG CGGAGCAGGG CGACGATCAG AGACGAAATG CTCACTATGA AGGTGGAGCC
    GTGTGCAGCC ATGCTAGATC TCTCTGGAGA CTGGAGCCTC TTCGCATTGG ATGGAGTGGT
    AGCCACATGA AGTGGGGTCA GTCATTCAGG GTGCGCCACA TCACAACAGG CAGGTATCTG
    TGTCTGGACG AAGAGAAAGG ACTCCTGATT GTGGACCCAG AAAAGGCAAA TACGAAGCAG
    TCAGCTTTCT GCTTTCGTGC ATCAAAGGAG AAGACAGAAG TAGCTCAGAA ACGTGATGTG
    GAGGGAATGG GGATCCCAGA GATCAAGTAT GGCGAGTCCA TGTGCTTTGT GCAGCACGTC
    GCTACAAGTC TGTGGCTCAC ATATGCCTCG GTGGATGCCA AGTCTGCTCG GCTGGGACCA
    CTGAAGAGGA AGGCTATCCT GCATCAGGAG GGTCACATGG ATGATGCTTT GACTGTGGCT
    CGCTCTCAGA CAGCTGAATC GCAGGCTGCC AGGATGATCT ACAACACGGC AGGACTTTTC
    ACTCAGTTCA TTAAGGGTTT GGACTCTCTA AGTGGGAAGA GTAAATCTCC AGGACCTTCC
    CCTTCTCTGC CTCTAGACGC CATAGTTCTG TCATTGCAGG ATCTCATCTT CTATTTCCGA
    CCTCCAGAAG AGGAGCTGGA ACATGAGGAG AAGCAGACCA AACTGCGCTC CTTAAGAAAC
    CGCCAGAATC TCTTCCAAGA GGAGGGCATG ATCACTCTTG TATTGGACTG TGTTGATCGG
    TTGAATGTCT ACAACACTTC AGCCCACTTC TCAGAATTTG CTGGAGAGGA CGCAGCCGAG
    TCGTGGAAAG AGATCGTCAA CTTATTTTAT GAGCTGCTGG CATCTCTGAT CAGAGGAAAC
    AGGGCAAACT GTGCTTTGTT CTGTGATAAT CTTGACTGGT TGGTCAGCAA ACTGGATCGT
    CTGGAGGCAT CCTCGGGTAT TCTGGAGGTG CTTTATTGTA TCCTAATTGA AAGCCCTGAG
    GTGCTTAACA TCATCCAGGA AAACCACATC AAATCTATCA TTGCACTTTT GGACAAACAT
    GGACGCAATC ACAAGGTTTT AGATGTTCTC TGCTCATTAT GCGTGTGTAA TGGTGTTGCT
    GTGAGGTCCA ATCAGAATCT GATCACTGAA AACCTGCTTC CCGGACGGGA TCTTCTCCTT
    CAGACCAACA TCATTAATTA TGTTACCAGT ATGAGACCCA ACATTTTTCT GGGCACGTGT
    GAAGGCTCGA CTCAGTATAA GAAGTGGTAC TATGAGTTGA TCGTTGACCA TGTGGAGTCT
    TTCCTTACCG CTCATCCATT TCACCTTCGT GTTGGCTGGG CTTTGACGGA GGGCTACAGC
    CCGTATCCAG GAGGTGGAGA AGGATGGGGA GGCAATGGAG TTGGAGATGA TCTACACTCG
    TATGGTTTCG ATGGGTTGAA TCTGTGGACA GGTCGTGTAC CACGTCAGGT GACGTCACCC
    AGTCAACACA TTCTCTCGGC TGAGGATGTG GTGAGCTGCT GTTTAGATCT AAGCGTTCCC
    AGCATCTCCT TTCGTATTAA TGGACATCCC GTTCAAGGCA TGTTCGAGAA CTTCAATCTG
    GATGGTCTTT TCTTCCCCGT GGTCAGCTTC TCAGCGGGGA TAAAAGTTCG TTTTCTCCTT
    GGCGGACGAC AAGGAGACTT CAAATTCCTC CCTCCTCCAG GTTATGCCCC GTGCTATGAG
    GCCGTTCTGC CCAAAGACAG ACTGCGGATT GAACCCATTA AAGAGTACAA GCACGACAAC
    GACGGTGTCC GAAACCTGCT GGGACCGACA CAGTCTCTTT CCCACACCGC CTTCACTCCC
    TGTCCGGTGG ACACTGTGCA GATTGTGCTT CCTCCACATC TAGAGCGCAT CAGAGAGAAA
    CTGGCCGAAA ATAGCCATGA GCTGTGGGCC GTCACTCGAA TTGAACAGGG TTGGACATAT
    GGACTATTTC GTGATGACAA CAAGAAGCTG CATCCCTGTC TAGTGGACTT CCAGAGCCTC
    CCGGATCCTG AGAAGAGCTA CAACCTGGCC ATGTCTGGAG AGACGCTGAA GACTCTTCTG
    GCTTTAGGGT GTCATGTAGG CATGGGTGAT GAAAAAGCAG AGGAGAACCT GAAGAAGATC
    AAACTTCCTA AAACGTATAT GCAGTGTAAC GGATACAAAC CTGCTCCACT GGATCTTAAC
    CATGTGAAGC TCACACCGAA TCAGAACACA CTTGTGGAAA GGCTGGCTGA AAATGGACAC
    AACGTCTGGG CTCGAGACCG TGTCCGCCAA GGGTGGACTT ACAGCATCAT TCAGGATATT
    GTGAACAAGC GCAACCCTCG TCTCGTGCCT TACATTCTGC TTGATGAAAA GACAAAAAAG
    ACCAATCGTG ACACTGTGTG TGCCGCTGTA CGGACTCTGA TTGGCTATGG CTACAATATT
    GAACCCCCTG ACCAGGAGAG CAGTGGTCAT GGGGGTGGCA ATGGCCGGAG TGATAAGATC
    CGTGTGTTCA GGGCAGAGAA ATCCTACGCA GTGACGCAGG GTAAATGGTA CTTTGAGTTC
    GAGGCAGTAA CAGTGGGAGA GATGAGAGTT GGGTGGGCAA GACCAAGCGT CCATGCAGAC
    AGAGAGCTGG GATCAGATGA CCTGGCCTAC GTGTTCAATG GCTTTAAGTC TCAGCGCTGG
    CACATTGGAA ATGAGCCGTT TGGCCGACAG TGGCAGTCTG GTGATGTCGT TGGCTGCATG
    ATAGACCTGA CGGAGCAGAA CATCATGTTT ACTTTGAATG GAGAGATGCT CATCAGTGAC
    TCTGGATCTG AGATGGCATT TAAAGACATT GAGATCGGAG AAGGCTTCAT CCCTGTATGC
    AGTCTGGGTA TGTCCCAGGT GGGTCGGATC AACCTTGGTC AAAATGTGAG CAGCCTGCGG
    TACTTTACAA TCTGTGGACT GCAGGAAGGT TTCGAGCCAT TTGCTATCAA CATGAAGAGA
    GATATTACAA TGTGGTTCAG CAAGAGCTTG CCCCAGTTTG TGCCCGTTCC AACAGATCAT
    CCTCATATTG AGGTGTCCCG TGTGGATGGA ACGGTGGAGA GCGCTCCCTG TCTGAAACTG
    ACTCACAGGA CATTCGGTTC TCAGAATGCC AACACAGACC TGTTGTTCTT CAGACTCAGT
    ATGCCGATTG AATTCCACGA AACCTTCAAG GTCCCGGTGG GAGCTTCACC CCTCACACGA
    ACCCTCACTA TCCCTGAGGA TGAGACTCTT GAGGTGGATC CCGAATCTGA ATTCGAGCTC
    CTGAAGAAGT CAGCCACTCG TAAGGAGCAA GATGAGAAAG AAAAAGAGCC GTCCGTCCCG
    AAAGAGCTGC CTGGAAGCAA AGAAAGCGAG AATGAGAAGG ACACCACCAC AGAGAAGAGC
    AAAAAGAGAG GATTTTTTTC CAAGGCGAAG AAGGCAGCGT TTATTGCCCC ACCACCAGTG
    GTTCCAACAA TGCCTCGTCT AATGGAGGAT GTTGTCCCAG ATGATCGAGA CGATGTTGAA
    ATTATCTCAA ACACAACAAC TTATTATTAT TCAGTCCGGG TGTTCGCCGG ACAGGAGCCC
    AGTGGAGTAT GGGTAGGCTG GGTGACTCCT GATTACCACT TGTACGACCT TCACTTTGAT
    CTTTGTAAAG TCAGAAACGT AACTGTTACA GTTGGAGACG ATAAAGGCAA TATTCATGAC
    AGTGTGAAAC GCAGTAATTG CTACATGGTG TGGGGTGGAG AGTTTAGCAG CTCACAGCAA
    ACCCGCATCA GTCAGGAGGA CTTTGTGATC GGTTGTTTGG TGGACCTGGA CACAGGACTG
    ATGACCTTTA CAGCCAATGG GAAAGAGATC AATACGTTCT ATCAGGTTGA ACCTAACACT
    AAGCTTTTCC CTGCTGTGTT TTGTTTGCCA TCAAGTCAGA ACATGTTACA AGTGGAACTC
    GGCAAACTGA AGAACATTAT GCCCATCTCA GCAGCCATGT TTCGCAGCGA CTGTAAGAAC
    CCGGTCCCTC AATGCCCTCC CAGGTTAGAT CTTCAGATGC TGACCCCTGT CATATGGAGC
    CGGATGCCTA ATCACTTTTT GTCGCCTGAA ACTGGCCGCG TGAGTGAGAG GCAGGGCTGG
    AAAGTGCAAT GCATGGAACC TCTCGTTATG ATGGCACTGC ACATCCCTGA AGAGAACAGG
    TGTATTGACA TCCTGGAACT GTCAGAACGT ACCGACCTGA TGAAGTTCCA TTACCACACA
    CTGAAACTGT ATGGCTCGGT TTGCGCTCTG GGGAACAATC GTGTCGCACA CGCCCTCTGC
    AGCCACGTGG ACGAATCGCA GCTCTTTTAC GCCATCGAAA ACACGTACCT GCCTGGCCCG
    ATCCGCAGTG GCTACTACGA CCTGCTCATC TCCATGCACC TAGAGTCTGC CAAACGCAAC
    CGACTCATGA CCAATAAGGA GTTCATTGTG CCCATGACGG ACGAGACTCG AAGCATCACC
    CTTTATTCGG ATGCTGAGAA GGCACATGCG TTACCTGGTG TCGGACTTAC CACATGTCTA
    CGCCCAAAAC TTCACTTTGC TCCAACTGGA TTTGTGGGCA CTAATGCTGA CCTTTACACC
    CTGAGCCCCA TGTTACCTCT TCAGATGCTT AAAGATCATG CCCTCAGCAT GCTTACAGAA
    GCTGTGCAGG ACGGTGGCCA AGCCATTCGA GATCCTGTAG GGGGCAGCGT TGAGTACCAC
    TTTGTGCCCA TCCTGAAGCT CATCAGCACC CTTCTCATTA TGGGTGTATT TGATGACGAC
    GATGTCAAGC ACATCCTGAA ACTGATCGAA CCATCCGTCT TCAGCAGCAA CAGTGAGACT
    CCAGCTGAAG AAACTGGAGA TCCTGAACAG CAGCTGGTCT GCAAAGAGGA AGGAGCGGAG
    GTAAAAGAGC AAGAGGAACC AGCAGACGGT GGAAATGATG GAGAGCTGCT GGATGAAGGG
    ATGGGAGAGG AAGAAGAGCT TGAGGAAGAG GAGGAGGAGG AAGATGAAGA GTATGAGGAG
    GAGGTGAAAA AAGAGGAGGA TGAACATGCT GCTGAAAAAA CAGACAGAGA GAAAACTACA
    GATGGAGAAA CTGAGAAGGA AACTGGTGCA GCAGAGGCTG AAGTAAGGGA GGAGGAGGAT
    GTTGGAGAAG GACTGCTGCA CATGAAGCTT CCAGAGTCTG TCAAACTCCA GATGTGTACT
    CTCCTGCAAT ATTTCTGTGA CTGTGAGCTG CGTCACAGAG TGGAAGCCAT CATCGCTTTC
    TCAGACAAGT TTGTTAACCA AGTGCAGACC AATCAGAGAC AGCGATATAA TGAGCTCATG
    CAAGCTTTTA CAATGAGTGC AGCAGAAACG GCCCGTAAGA CCCGCGAATT CCGCTCCCCT
    CCACAGGAAC AGGTGAACAT GTTGATGAAC TTTAAGAACT GTCCAGAGGA AGAAGATTGC
    CCCGTTCCAG AGGATGTCCG GAATGGCCTC CTGACATTTC ACCATGACCT TTTAGCTCAC
    TGTGGTGTGC ACATTGAAGA GGAGGAACAG GAAGAAGAGG TGGACACTTC TCTCAGTGGG
    CGCCTACGCA GACTGGTGGA AAAAGTTAAA AGCTTGCGAA AAAAGCCAGA AGCTGAGCCT
    GAGCCTGAAG AAGATAAAAA ACCAAGTACT CTGCAGGAGC TGATCTCCCA CACCATGATC
    TACTGGGCTC AAGAGTCTTT CATCCAGAAC CCTGAGCTGG TGCGCCTCAT GTTTAGTCTG
    CTACACCGGC AGTATGATGC TTTGGGTGAG CTGATTCGAG CTTTGCCCAA AGCCTATATC
    ATTAATGCTG TTTCTATACA AGACACAATG GAGCTCCTGG AGTGTCTGGG ACAGATACGC
    TCCTTGCTCA TCGTTCAGAT GGGACCAGAG GAAGAGAGGC TGATGATCCA GAGTATCGGA
    AATATTATGA GTAACAGGGT ATTTTACCAG CACCCCAACT TGATGAGAGC TCTGGGAATG
    CATGAGACAG TCATGGAGGT TATGGTGAAC GTACTCGGAG GCGGAGATTC CAAGGAGATC
    AGATTCCCTC GAATGGTTAC CAACTGCTGT CGTTTCCTGT GTTATTTCTG TCGTATCAGC
    AGACAGAATC AGCGCTCGAT GTTTGATCAT CTGGGCTACC TCCTGCAGAA CAGTGGCATT
    GGGCTGGGAA TGCGTGGCTC CACTCCTCTT GATGTTGCTG CTGCTTCCTG TATCGATAAT
    AATGAGCTTG CCCTGGCTCT CCAAGAGCAA GACCTGGAGA TGGTAGTAAA GTACCTGGCA
    GGCTGTGGTC TGCAGAGCTG CCCTATGCTT TTGTCAAAGG GTTACCCGGA TATTGGATGG
    AACCCAGTTG GAGGAGAAAA GTATCTGGAT TTCCTGCGCT TTGCTGTGTT TGTGAATGGA
    GAGAGTGTGG AGGAGAACGC TAATGTGGTG GTCCGGCTGC TGATCAGGAG GCCTGAGTGC
    TTTGGTCCTG CACTGAGAGG AGAAGGGGGC AATGGTCTGC TGGCTGCCAT TGAAGAAGCC
    ATCAAGATCT CAGAGGACCC TGCAAGAGAT GGACCCACTG TTAAAAAAGA CAGACGCTTC
    CCGATGTTTG GAGGTGAAGA GCAGCATGAG GAGAACAGAG TGCACCTAGG AAATGCCATC
    ATGTCATTTT ACTCAGCTCT TATTGATCTG CTGGGTCGCT GTGCTCCTGA GATGCATTTG
    ATCCAGGCTG GTAAAGGTGA AGCTTTGAGA ATCAGGGCTA TTCTTCGGTC GCTTGTGCCC
    ATTGAGGATC TGGTCGGTGT GATCAGCTTG TCAGTCCAGA TTCCTGCTTT TGGAAAAGAT
    AACAGCATTA TTGAGCCGAA GATGTCTGCT AGTTTTGTTC CTGATCATAA AGCACCAATG
    GTCCTGTTCC TGGACAGAGT TTATGGCATC GACAACCAGG ACTTCCTATT GCATGTACTA
    GAAGTGGGTT TCCTGCCTGA TATGAGGGCG GCAGCCTCCT TAGACACAGC TGCATTCAGC
    ACTACAGAAA TGGCCTTGGC TCTGAACCGT TACCTGTGCT CCGCCGTGCT TCCTCTCATC
    ACTAAATGTG CTCCTTTGTT CGCCGGGACG GACCACCGAG CCATCATGAT CGACTCCATG
    CTTCACACCA TCTACAGGCT GTCTCGAGGG CGCTCGCTAA CGAAAGCACA AAGAGATGTT
    ATTGAGGAAT GCCTCATGTC TTTGTGCAGG TATCTGCGCC CTTCCATGCT CCAGCACCTT
    CTCAGGCGGC TAGTATTTGA TGTGCCTATT CTGAATGAGT ATGCCAAAAT GCCCCTTAAG
    CTGCTTACCA ATCATTATGA GCGTTGTTGG AAATATTATT GCTTGCCTAA TGGCTGGGCT
    AACTTTGGCG TATCATCAGA AGAGGAGCTT CATCTGACCC GTAAACTCTT TTGGGGGATC
    TTTGAATCTC TGGCTCACAA GAAATTCGAT GCAGAGCTGT TCAAAATCGC CATGCCTTGT
    ATATGTGCGA TCGCTGGTGC AATTCCTCCT GATTATGTGG ACGCCAGCTA CTCCTCCAAA
    ACCGAAAAAA AGGCTTCTGT TGATGCTGAA GGGAACTTTG ACCCCAAACC TGTTGATACC
    ACTAACACTA TAATTCCCGA GAAGTTGGAC GGGTTCATCA ACAGATATGC AGAATATACG
    CACGACAGAT GGGCTTTTGA AAAAATTCAA AACAACTGGA CATTTGGTGA GGTGATGGAT
    GAAAATGCCA AGACTCATCC CATGCTCAGA CCTTACAAAA CATTCTCTGA AAAGGATAAA
    GAAATCTACC GCTGGCCTAT TAAAGAGTCC ATCAAGGCCA TGATTGCCTG GGAGTGGACA
    CTGGACAAAG CCAGAGATGG AGAGGATGAG AAAGTTGAGA AGAAAACAGC CCGTAAGATC
    TCACAGACTG CACAGGCAAC CTATGACCCC AGTCATGGCT ATAGCCCCCA ACCTATTGAT
    ATCTCAGGCA TGACACTGTC CAGAGAGCTA CAGTCCATGG CAGAGCAGCT CGCAGAAAAC
    TACCACAACA CCTGGGGCCG TAAGAAGAAA ATGGAATTAC AAGCAAAAGG AGGAGGTACA
    CACCCTTTGC TTGTGCCTTA TGACACACTG ACGGCTAAAG AAAAGGCACG AGATAGAGAG
    AAAGCGTATG AGCTACTCAA GTTTCTGCAG CTCAATGGAT ATGCTGTGAC AAGAGGGCTC
    AAAGACATGG AAAGTGACAT TTCATCTATC GAGAAGCGTT TTGCGTATGG CTTCCTACAG
    AAGCTTCTGA AGTGGATGGA CATTGCTCAG GAGTTCATAG CACATCTTGA GGCGGTTGTG
    AGCAGCGGTC GTGTGGAAAA GTCACCACAT GAGCAAGAAA TCAAGTTCTT TGCCAAGATT
    CTTTTGCCTT TGATAAATCA GTATTTTAAA AACCACTGCC TTTACTTCCT CTCCACACCG
    GCCAAAATCC TAGGCAGCGG CGGACACGCC TCAAACAAGG AAAAGGAGAT GATTGCAAGC
    ATTTTCTGTA AGATGTCTGC CCTGGTGCGA CACAGAGTGT TTCTCTTTGG CACGGATGCG
    CCTGCTATTG TCAACTGCCT TCATATCCTC GCACGTTCGC TTGATGCCAG GACGGTGATG
    AAATCCGGGC CTGAGATTGT GAAAGCAGGA CTGCGTTTGT TCTTTGAGAG TGCAGCTGAT
    GACATTGAAA AGATGGTGGA GAATCTCAAA CTTGGCAAAG TGTCCAAAGG CAATCAGCCA
    GTGAAAGGTG TGTCCCAGAA TATAAACTAC ACCACAACGG CTCTGCTTCC TGTCCTCACA
    TCACTGTTTG ACCACATCGC TCAACACCAA TTCGGAGATG ATGTTATCCT GGATGATTTG
    CAGATGTCCT GCTACCGAAT CATGTGTAGC ATCTACTCTC TTGGGACTGT AAAAACTCCA
    CATGCAGAGA GGCATAGACC AGCCCTTGGA GAATGTCTCG CTCATCTCGC TGCAGCAATG
    CCTGTTGCCT ACCTGGAGCC GCATCTTAAT GAATACAACG CCTTTTCAGT CTACACTACC
    AAGACACCGA GAGAAAGAGC TATCCTTGGC CTTCCAAACC AAGTTCAAGA GTTGTGTTTG
    GACATTCCTG AGCTTGACGT TTTACTGAAG GAGATCGGAG ACCTGGCTGA ATCAGGAGCT
    CGATACACTG AAATGCCACA TGTGATTGAG GTCACTCTGC CCATGCTGTG TAACTATCTG
    CCCCGCTGGT GGGAGAGAGG ACTGGAGAAC TTCCCGGATC TAGAGGGTAA GCTGTGCACC
    GACGTCACCT CCGAATCCCT CAACCAGCTT CTGGGAAGCA TCATGAAAAT CGTGGTCAAC
    AACTTGGGAA TCGACGAAGC CTCCTGGATG AAGAGACTTG CAGTGTTCTC CCAACCCATT
    GTGAGCCGAG CCAAACCAGA GATGCTCAAA TCCCACTTCA TCCCCACCAT GGAGAAGCTG
    AAGAAGCGAA CCTCTAAAGT TGTAGCCGAG GAGGAGCATC TGCGTTTGGA GGGAAAGTCT
    GAGGGAGATG AGGAAGAGGG AACTATCAGA GACGAGTTTG CAGTTCTCTG TAGAGACCTC
    TATGCTCTAT ATCCTCTTCT CATCCGCTAT GTGGATAACA ACAGAGCAAG ATGGCTGACG
    TTTCGAGACC CAGATGCTGA GGAGTTGTTC CGCATGGTGG GAGAGGTCTT CATCTTCTGG
    TCAAAATCTC ACAATTTCAA GCGAGAGGAG CAAAACTTTG TGGTGATGAA CGAAATCAAC
    AATATGTCCT TTCTCACTGC TGACAGTAAG AGCAAGATGA GCAAGGCCGG GGACTCTGAG
    GCTGGAGGCT CGGATGTGGA GCGTACCAAG AAAAAGAGAC GGGGGGATCG ATACTCTGTG
    CAAACGTCCC TCATTGTGGC CGCACTGAAG AAAATGCTTC CCATTGGACT TAACATGTGT
    TCTCCTGCAG ACCAGGAGCT GATTAATCTG GCAAAGACAC GTTATTCTCT GAAAGACACA
    GATGAGGAGG TCAGAGAGTT CTTGCAAAAT AACCTGCACC TTCAGGGAAA GGTTGATAAC
    CCATCTATGA GGTGGCAGAT GGCACTGTAT AAGGAAATGG CAGGAAAGGC TGAAGATGCA
    GATGCCCCAG AGAATGTGGT CAAGAGGGTT CAGGAGGTCT CTGCGGTCCT TTACCACATT
    GAAGTGACGG AGCACCCTTT CAAGTCCAAA AAGATGGTTT GGCACAAACT GCTGTCGAAG
    CAGCGGCGTA GAGCGGTGGT GGCCTGTTTC AGGATGACTC CACTTTACAA CTTGCCCAGA
    CACAGAGCAT CTAATATGTT CCTGGATGGG TACAAGCGCA ACTGGATAAG AAATGAAGGC
    TATTCCTTTG AGGACAGAAT GATTGATGAC CTGTCTAAAG CATTGGAGGA GGAAGAAGAG
    GAGGAAGAGG AAAAGGAGAC AAAGCCAGAC CCTCTTCATC AGCTCATCCT TCACTTTAGC
    CGCACAGCAC TCACTGAGAA AAGTAAACTG GATATAGATC ACTTATACAT GGCATATGCT
    GATATCATGG CTAAGAGTTG CCACATGGAT GAAGAGGATG AGGGTGGAGA GGAGGAGAAT
    GAGGGTGGAG AGGAGGAGAT GTCCTTTGAG GAAAAGGAAA TGGAAAAGCA AAGACTGCTC
    TACCAGCAGT CGCGTCTACA CAACCGAGGG GCTGCTGAAA TGGTCCTGCA GATGATCAGT
    GCCTGCAAGG GTGAGACTGG TTGTATGGTC TCTTCCACCC TAAAACTTGG CATTTCTATT
    TTAAATGGCG GGAACTGTGA AGTTCAGCAG AAAATGCTGG ACTACCTGAA AGACAAGAAA
    GATGTGGGTT TCTTTCTCAG TGTTCAGGCT TTAATGCAGA CCTGCAGTGT TTTGGACCTG
    AATGCCTTTG AGAGGCAGAA TAAAGCTGAG GGTCTAGGTA TGGTGTCAGA AGAGGGTACA
    AACATGAAGC TAGAACGGGG TGAGAAGGTC ATGGCTGATG ACGAGTTCAC TTGCGACCTC
    TTCCGCTTTC TCCAGCTCCT CTGTGAGGGT CACAACAATG ACTTCCAAAA TTACCTCAGA
    ACACAGACAG GCAGTACCAC CACCATCAAT GTCATTATCT GTACAGTGGA TTACCTGCTT
    AGACTACAGG AGTCCATCAG TGATTTCTAC TGGTATTACT CTGGAAAAGA CATCATAGAT
    GAACCAGGAA AGCGAAACTT CTCTAAAGCT ATGACTGTGG CCAAACAGGT TTTCAACAGT
    CTTACTGAGT ATATCCAGGG TCCCTGCACA GGAAACCAGC AGTCCCTGGC TCACAGTCGC
    TTATGGGATG CTGTGGTGGG CTTTCTGCAC GTGTTTGCTC ATATGATGAT GAAACTGGCT
    CAGGGAAGAG ATTCCAGTCA GATTGGTCTT CTGAAAGAGT TGTTGGACCT TCAGAAAGAC
    ATGGTGGTCA TGTTATTGTC CTTGTTGGAG GGTAATGTTG TAAATGGCAC CATTGCTCGC
    CAGATGGTGG ACATGCTAGT GGAATCCTCC AGCAATGTAG AAATGATCCT TAAGTTCTTC
    GACATGTTCC TCAAGCTGAA GGACATTGTT GCGTCTGACG CGTTTCGTGA CTACGTGACC
    GACCCTAGAG GTTTGATATC CAAGAAAGAC TTTCAGAAAT CCATGGACAG CCAGAAACAG
    TACACACCTT CAGAGATCCA ATTCCTATTG TCATGCTCTG AGGCTGATGA GAATGAGATG
    ATCAATTACG AGGAGTTTGC AAGTCGCTTT CAAGAGCCGG CCAAAGACAT TGGTTTTAAC
    ATAGCGGTGC TGCTAACCAA CCTCTCCGAA CATGTTCCTC ACGATACAAG ACTGCAGAAC
    TTCCTCGAAC AAGCCGAGAG CGTCCTGAAC TATTTCCGCC CATTCTTGGG TCGCATCGAG
    ATCATGGGCG CCAGCAGGAA AATCGAGAGA ATCTACTTCG AGATCAGCGA GGTGAATCGC
    AAGCAGTGGG AGATGCCTCA AGTCAAAGAA TCAAAGAGAC AGTTCATATT CGATGTAGTC
    AATGAAGGCG GCGAATCGGA GAAGATGGAG CTTTTCGTCA ACTTCTGCGA GGACACCATA
    TTCGAGATGA ACATTGCGTC GCAGATTTCA GAACAGGACG AGGAGGAGAA AGAAGAGGAT
    GATATTGAGG AAGCTGAAGG AGGAGATGCT GGTGATGAAG AGGGGGGTGG CGAGGAGGGA
    CAACCAGAAT CAAGCTCTGC ATTTGCCGAT TTCATTCAAA GCATCATGAA TTTTCTGGGT
    ATGTTCACCT TCAGGAATAT TCGCAGAAAG TACCGCAGAC TCCGCAAGAT GACCATTAAG
    GAGATCGTGG TAGGGCTTGC TACCTTCATG TGGACGATTC TCTTGGGCAT CCTCCATTTC
    ATTTACAGTG TTTGTAAAGG CTTCTTCCTC CTCATCTGGC ACACGCTGTT TGGAGGAGGT
    TTAGTTGAAG GGGCCAAAAA CATCACAGTA ACTGAGTTAC TAGCCAGCAT GCCAGATCCC
    ACACAGGACG AGGTGCATGG GGACTTACCT GGAGAACCGA GGGGAGGAGA GGAACAGGAG
    ACTGGAGGGA TAATGGACTC GATGGATGCT GGAGGGGGAG AGGAAGAGGA TGAGGATATC
    CAGGAGAAAG ATGGTGGAAA GATTCCTGGT ATAGATACAC CTGGTGGACT TGGAGATATG
    GGTGATACAA CTCCTGTAGA ACCGCCGACT CCCGAGGGTA CCCCTTTGTT GAAGAGGAAA
    GCGCAACCAG ATGAAGCAGG AGCTGAACAG CCTCCTGCTA TTGAAGAGCC TCCTCCTGAG
    CCTGAGAAAG CAGACATTGA GAATGGTGAG AAGGCTGAGA AGGAAGTGGA GGTCAAGCCT
    GAGCCAATAC CAGAAGAACC GAAACCTCAG AGAGCCACCA AAGCCAAGAA GGAGAAGAAA
    TCAGTCAAGG GTGCAGGATT TGAGCTCTGG AATGAGCTGG ATGTGCAAAG AAATAAATTC
    ATGAACTGCT TGTCTCGTAA CTTTTACAAC ATTCGTTTCC TGGCTCTGTT CCTTGCCTTT
    GCCTTGAACT TCATTCTTCT CTTCTACAAG GTATCTGATA CTCCCCCCAG TTCTGATGAA
    TTTGAAGGTT CTGGCATCTT TGAAGGCTCT GGGCTGTTTG AAGGCTCTGC GGAGGATTTT
    GAGGGCTCTG GTGGAGAGGA TGGAGGTGAT GATGAGGATG AAGAAGGACC TGTGTATTAT
    TTTCTGGAAG AAAGCACGGG ATACATGCAG CCCACTCTGT CCTTCCTGGC CATTTTGCAC
    ACCGTCATTG CTTTCATATC CATCATTGGG TACAACTGCC TCAAGATTCC ACTGGTCATC
    TTTAAGAGAG AGAAAGAGTT GGCGAGAAAG CTGGAGTTTG ATGGCCTTTA CATCACTGAA
    CAGCCGGCAG ATGATGACAT TAAAGGCCAG TGGGACAGAC TGGTGTTGAA CACTCCCTCT
    TTTCCCACCA ATTACTGGGA CAAATTTGTG AAGCGAAAGG TTCTGGATAA ATATGGAGAT
    ATTTATGGAA GAGAGAGAAT CGCAGAGCTG CTGGGTATGG ATCTGGCATC TCTGGATGTC
    AGCAAACAAC AGACAGACAA GAAACCAGAA GAGCCAGACA ACTCTATGCT TGCATGGTTC
    ACTTCCATTG ACATCAAGTA TCAGATCTGG AAATTTGGAG TTGTGTTCAC AGACAATACT
    TTCCTCTACC TGGTGTGGTA CACAGTGATG TCCCTTCTTG GACATTACAA CAACTTTTTC
    TTTGCTTGTC ACCTGCTGGA CATCGCAATG GGTGTTAAGA CCCTGCGTAC AATTCTTTCC
    TCCGTCACAC ATAATGGAAA ACAGCTGATG ATGACTGTGG GTTTGCTGGC TGTGGTTGTG
    TACCTCTACA CTGTGGTGGC CTTCAACTTC TTCAGGAAGT TCTACAATAA GAGCGAGGAT
    GAGGATGAGC CGGATATGAA GTGTGACGAC ATGATGACGT GTTATTTGTT CCACATGTAT
    GTGGGTGTAC GGGCAGGTGG AGGTATAGGA GATGAGATCG AGGACCCTGC TGGAGATGAG
    TATGAGCTCT ATCGGGTGGT CTTCGACATC ACCTTCTTCT TCTTTGTCAT CGTCATCCTG
    CTGGCCATCA TTCAGGGTCT GATCATTGAT GCCTTTGGTG AACTGAGAGA CCAGCAAGAG
    CAAGTGAAAG AGGACATGGA GACCAAATGC TTTATATGTG GCATTGGGAG CGACTACTTT
    GATACAACGC CCCATGGCTT TGAGACCCAT ACGCTTGAAG AACACAACCT GGCCAATTAC
    ATGTTTTTCC TGATGTACTT AATCAACAAG GATGAAACAG AGCACACTGG CCAGGAATCA
    TACGTGTGGA AGATGTACCA GGAGAGATGC TGGGATTTCT TCCCTGCAGG AGACTGCTTC
    AGAAAGCAAT ATGAAGACCA GCTTGGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021335080.1
    CDS199..15408
    Translation

    Target ORF information:

    RefSeq Version XM_021479405.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio ryanodine receptor 1a (skeletal) (ryr1a), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021479405.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    13261
    13321
    13381
    13441
    13501
    13561
    13621
    13681
    13741
    13801
    13861
    13921
    13981
    14041
    14101
    14161
    14221
    14281
    14341
    14401
    14461
    14521
    14581
    14641
    14701
    14761
    14821
    14881
    14941
    15001
    15061
    15121
    15181
    ATGGCAGAAG GAGCTGAGGG AGAAGAGGAG ATCCAATTCC TAAGAACAGA TGACCAGGTG 
    GTGCTACAGT GTACAGCTAC TATCCTGAAG GAGCAGATTA AACTCTGTCT GTCCTGTGAG
    GGTTTTGGGA ATCGCCTGTG TTTTTTAGAA ACCACATCTA ATGCTCAGAA TGTTCCTCCT
    GATCTGGCAA TATGCTGCTT TATCCTGGAG CAGTCTTTAT CTGTGCGGGC CCTGCAGGAG
    ATGCTGGCTA ACACTACTGA GCTTAATGAG GCAGTCGATT TGGATAAATG GTCTTCACAA
    GGAGGAGGCC ACCGCACGTT GTTGTATGGC CACGCTATCC TACTCAGACA TACTCACTCT
    GGCATGTACC TTAGTTGCTT GACCACATCA AGATCCCTCA CAGACAAACT GGCCTTTGAT
    GTGGGCTTGC AGGAGGACTC CACAGGTGAG GCCTGTTGGT GGACCATTCA CCCTGCATCC
    AAGCAGAGGT CAGAGGGTGA GAAGGTCAGA GTGGGCGATG ACATTATTCT GGTCAGCGTG
    TCCTCTGAGA GATATCTGCA CCTCTCCTAT GCCAGCGGAG ACCTGATGGT GGACGCTTCT
    TTCATGCAGA CCCTTTGGAA CATGAACCCA GTCTGTTCAG GCTGTGAGCT GGCAGAGGGG
    TTTCTGACTG GAGGGCATGT TCTTCGTCTA TTTCACGGGC ACATGGATGA GTGTTTAACA
    ATTCCTGGCG CGGAGCAGGG CGACGATCAG AGACGAAATG CTCACTATGA AGGTGGAGCC
    GTGTGCAGCC ATGCTAGATC TCTCTGGAGA CTGGAGCCTC TTCGCATTGG ATGGAGTGGT
    AGCCACATGA AGTGGGGTCA GTCATTCAGG GTGCGCCACA TCACAACAGG CAGGTATCTG
    TGTCTGGACG AAGAGAAAGG ACTCCTGATT GTGGACCCAG AAAAGGCAAA TACGAAGCAG
    TCAGCTTTCT GCTTTCGTGC ATCAAAGGAG AAGACAGAAG TAGCTCAGAA ACGTGATGTG
    GAGGGAATGG GGATCCCAGA GATCAAGTAT GGCGAGTCCA TGTGCTTTGT GCAGCACGTC
    GCTACAAGTC TGTGGCTCAC ATATGCCTCG GTGGATGCCA AGTCTGCTCG GCTGGGACCA
    CTGAAGAGGA AGGCTATCCT GCATCAGGAG GGTCACATGG ATGATGCTTT GACTGTGGCT
    CGCTCTCAGA CAGCTGAATC GCAGGCTGCC AGGATGATCT ACAACACGGC AGGACTTTTC
    ACTCAGTTCA TTAAGGGTTT GGACTCTCTA AGTGGGAAGA GTAAATCTCC AGGACCTTCC
    CCTTCTCTGC CTCTAGACGC CATAGTTCTG TCATTGCAGG ATCTCATCTT CTATTTCCGA
    CCTCCAGAAG AGGAGCTGGA ACATGAGGAG AAGCAGACCA AACTGCGCTC CTTAAGAAAC
    CGCCAGAATC TCTTCCAAGA GGAGGGCATG ATCACTCTTG TATTGGACTG TGTTGATCGG
    TTGAATGTCT ACAACACTTC AGCCCACTTC TCAGAATTTG CTGGAGAGGA CGCAGCCGAG
    TCGTGGAAAG AGATCGTCAA CTTATTTTAT GAGCTGCTGG CATCTCTGAT CAGAGGAAAC
    AGGGCAAACT GTGCTTTGTT CTGTGATAAT CTTGACTGGT TGGTCAGCAA ACTGGATCGT
    CTGGAGGCAT CCTCGGGTAT TCTGGAGGTG CTTTATTGTA TCCTAATTGA AAGCCCTGAG
    GTGCTTAACA TCATCCAGGA AAACCACATC AAATCTATCA TTGCACTTTT GGACAAACAT
    GGACGCAATC ACAAGGTTTT AGATGTTCTC TGCTCATTAT GCGTGTGTAA TGGTGTTGCT
    GTGAGGTCCA ATCAGAATCT GATCACTGAA AACCTGCTTC CCGGACGGGA TCTTCTCCTT
    CAGACCAACA TCATTAATTA TGTTACCAGT ATGAGACCCA ACATTTTTCT GGGCACGTGT
    GAAGGCTCGA CTCAGTATAA GAAGTGGTAC TATGAGTTGA TCGTTGACCA TGTGGAGTCT
    TTCCTTACCG CTCATCCATT TCACCTTCGT GTTGGCTGGG CTTTGACGGA GGGCTACAGC
    CCGTATCCAG GAGGTGGAGA AGGATGGGGA GGCAATGGAG TTGGAGATGA TCTACACTCG
    TATGGTTTCG ATGGGTTGAA TCTGTGGACA GGTCGTGTAC CACGTCAGGT GACGTCACCC
    AGTCAACACA TTCTCTCGGC TGAGGATGTG GTGAGCTGCT GTTTAGATCT AAGCGTTCCC
    AGCATCTCCT TTCGTATTAA TGGACATCCC GTTCAAGGCA TGTTCGAGAA CTTCAATCTG
    GATGGTCTTT TCTTCCCCGT GGTCAGCTTC TCAGCGGGGA TAAAAGTTCG TTTTCTCCTT
    GGCGGACGAC AAGGAGACTT CAAATTCCTC CCTCCTCCAG GTTATGCCCC GTGCTATGAG
    GCCGTTCTGC CCAAAGACAG ACTGCGGATT GAACCCATTA AAGAGTACAA GCACGACAAC
    GACGGTGTCC GAAACCTGCT GGGACCGACA CAGTCTCTTT CCCACACCGC CTTCACTCCC
    TGTCCGGTGG ACACTGTGCA GATTGTGCTT CCTCCACATC TAGAGCGCAT CAGAGAGAAA
    CTGGCCGAAA ATAGCCATGA GCTGTGGGCC GTCACTCGAA TTGAACAGGG TTGGACATAT
    GGACTATTTC GTGATGACAA CAAGAAGCTG CATCCCTGTC TAGTGGACTT CCAGAGCCTC
    CCGGATCCTG AGAAGAGCTA CAACCTGGCC ATGTCTGGAG AGACGCTGAA GACTCTTCTG
    GCTTTAGGGT GTCATGTAGG CATGGGTGAT GAAAAAGCAG AGGAGAACCT GAAGAAGATC
    AAACTTCCTA AAACGTATAT GCAGTGTAAC GGATACAAAC CTGCTCCACT GGATCTTAAC
    CATGTGAAGC TCACACCGAA TCAGAACACA CTTGTGGAAA GGCTGGCTGA AAATGGACAC
    AACGTCTGGG CTCGAGACCG TGTCCGCCAA GGGTGGACTT ACAGCATCAT TCAGGATATT
    GTGAACAAGC GCAACCCTCG TCTCGTGCCT TACATTCTGC TTGATGAAAA GACAAAAAAG
    ACCAATCGTG ACACTGTGTG TGCCGCTGTA CGGACTCTGA TTGGCTATGG CTACAATATT
    GAACCCCCTG ACCAGGAGAG CAGTGGTCAT GGGGGTGGCA ATGGCCGGAG TGATAAGATC
    CGTGTGTTCA GGGCAGAGAA ATCCTACGCA GTGACGCAGG GTAAATGGTA CTTTGAGTTC
    GAGGCAGTAA CAGTGGGAGA GATGAGAGTT GGGTGGGCAA GACCAAGCGT CCATGCAGAC
    AGAGAGCTGG GATCAGATGA CCTGGCCTAC GTGTTCAATG GCTTTAAGTC TCAGCGCTGG
    CACATTGGAA ATGAGCCGTT TGGCCGACAG TGGCAGTCTG GTGATGTCGT TGGCTGCATG
    ATAGACCTGA CGGAGCAGAA CATCATGTTT ACTTTGAATG GAGAGATGCT CATCAGTGAC
    TCTGGATCTG AGATGGCATT TAAAGACATT GAGATCGGAG AAGGCTTCAT CCCTGTATGC
    AGTCTGGGTA TGTCCCAGGT GGGTCGGATC AACCTTGGTC AAAATGTGAG CAGCCTGCGG
    TACTTTACAA TCTGTGGACT GCAGGAAGGT TTCGAGCCAT TTGCTATCAA CATGAAGAGA
    GATATTACAA TGTGGTTCAG CAAGAGCTTG CCCCAGTTTG TGCCCGTTCC AACAGATCAT
    CCTCATATTG AGGTGTCCCG TGTGGATGGA ACGGTGGAGA GCGCTCCCTG TCTGAAACTG
    ACTCACAGGA CATTCGGTTC TCAGAATGCC AACACAGACC TGTTGTTCTT CAGACTCAGT
    ATGCCGATTG AATTCCACGA AACCTTCAAG GTCCCGGTGG GAGCTTCACC CCTCACACGA
    ACCCTCACTA TCCCTGAGGA TGAGACTCTT GAGGTGGATC CCGAATCTGA ATTCGAGCTC
    CTGAAGAAGT CAGCCACTCG TAAGGAGCAA GATGAGAAAG AAAAAGAGCC GTCCGTCCCG
    AAAGAGCTGC CTGGAAGCAA AGAAAGCGAG AATGAGAAGG ACACCACCAC AGAGAAGAGC
    AAAAAGAGAG GATTTTTTTC CAAGGCGAAG AAGGCAGCGT TTATTGCCCC ACCACCAGTG
    GTTCCAACAA TGCCTCGTCT AATGGAGGAT GTTGTCCCAG ATGATCGAGA CGATGTTGAA
    ATTATCTCAA ACACAACAAC TTATTATTAT TCAGTCCGGG TGTTCGCCGG ACAGGAGCCC
    AGTGGAGTAT GGGTAGGCTG GGTGACTCCT GATTACCACT TGTACGACCT TCACTTTGAT
    CTTTGTAAAG TCAGAAACGT AACTGTTACA GTTGGAGACG ATAAAGGCAA TATTCATGAC
    AGTGTGAAAC GCAGTAATTG CTACATGGTG TGGGGTGGAG AGTTTAGCAG CTCACAGCAA
    ACCCGCATCA GTCAGGAGGA CTTTGTGATC GGTTGTTTGG TGGACCTGGA CACAGGACTG
    ATGACCTTTA CAGCCAATGG GAAAGAGATC AATACGTTCT ATCAGGTTGA ACCTAACACT
    AAGCTTTTCC CTGCTGTGTT TTGTTTGCCA TCAAGTCAGA ACATGTTACA AGTGGAACTC
    GGCAAACTGA AGAACATTAT GCCCATCTCA GCAGCCATGT TTCGCAGCGA CTGTAAGAAC
    CCGGTCCCTC AATGCCCTCC CAGGTTAGAT CTTCAGATGC TGACCCCTGT CATATGGAGC
    CGGATGCCTA ATCACTTTTT GTCGCCTGAA ACTGGCCGCG TGAGTGAGAG GCAGGGCTGG
    AAAGTGCAAT GCATGGAACC TCTCGTTATG ATGGCACTGC ACATCCCTGA AGAGAACAGG
    TGTATTGACA TCCTGGAACT GTCAGAACGT ACCGACCTGA TGAAGTTCCA TTACCACACA
    CTGAAACTGT ATGGCTCGGT TTGCGCTCTG GGGAACAATC GTGTCGCACA CGCCCTCTGC
    AGCCACGTGG ACGAATCGCA GCTCTTTTAC GCCATCGAAA ACACGTACCT GCCTGGCCCG
    ATCCGCAGTG GCTACTACGA CCTGCTCATC TCCATGCACC TAGAGTCTGC CAAACGCAAC
    CGACTCATGA CCAATAAGGA GTTCATTGTG CCCATGACGG ACGAGACTCG AAGCATCACC
    CTTTATTCGG ATGCTGAGAA GGCACATGCG TTACCTGGTG TCGGACTTAC CACATGTCTA
    CGCCCAAAAC TTCACTTTGC TCCAACTGGA TTTGTGGGCA CTAATGCTGA CCTTTACACC
    CTGAGCCCCA TGTTACCTCT TCAGATGCTT AAAGATCATG CCCTCAGCAT GCTTACAGAA
    GCTGTGCAGG ACGGTGGCCA AGCCATTCGA GATCCTGTAG GGGGCAGCGT TGAGTACCAC
    TTTGTGCCCA TCCTGAAGCT CATCAGCACC CTTCTCATTA TGGGTGTATT TGATGACGAC
    GATGTCAAGC ACATCCTGAA ACTGATCGAA CCATCCGTCT TCAGCAGCAA CAGTGAGACT
    CCAGCTGAAG AAACTGGAGA TCCTGAACAG CAGCTGGTCT GCAAAGAGGA AGGAGCGGAG
    GTAAAAGAGC AAGAGGAACC AGCAGACGGT GGAAATGATG GAGAGCTGCT GGATGAAGGG
    ATGGGAGAGG AAGAAGAGCT TGAGGAAGAG GAGGAGGAGG AAGATGAAGA GTATGAGGAG
    GAGGTGAAAA AAGAGGAGGA TGAACATGCT GCTGAAAAAA CAGACAGAGA GAAAACTACA
    GATGGAGAAA CTGAGAAGGA AACTGGTGCA GCAGAGGCTG AAGTAAGGGA GGAGGAGGAT
    GTTGGAGAAG GACTGCTGCA CATGAAGCTT CCAGAGTCTG TCAAACTCCA GATGTGTACT
    CTCCTGCAAT ATTTCTGTGA CTGTGAGCTG CGTCACAGAG TGGAAGCCAT CATCGCTTTC
    TCAGACAAGT TTGTTAACCA AGTGCAGACC AATCAGAGAC AGCGATATAA TGAGCTCATG
    CAAGCTTTTA CAATGAGTGC AGCAGAAACG GCCCGTAAGA CCCGCGAATT CCGCTCCCCT
    CCACAGGAAC AGGTGAACAT GTTGATGAAC TTTAAGAACT GTCCAGAGGA AGAAGATTGC
    CCCGTTCCAG AGGATGTCCG GAATGGCCTC CTGACATTTC ACCATGACCT TTTAGCTCAC
    TGTGGTGTGC ACATTGAAGA GGAGGAACAG GAAGAAGAGG TGGACACTTC TCTCAGTGGG
    CGCCTACGCA GACTGGTGGA AAAAGTTAAA AGCTTGCGAA AAAAGCCAGA AGCTGAGCCT
    GAGCCTGAAG AAGATAAAAA ACCAAGTACT CTGCAGGAGC TGATCTCCCA CACCATGATC
    TACTGGGCTC AAGAGTCTTT CATCCAGAAC CCTGAGCTGG TGCGCCTCAT GTTTAGTCTG
    CTACACCGGC AGTATGATGC TTTGGGTGAG CTGATTCGAG CTTTGCCCAA AGCCTATATC
    ATTAATGCTG TTTCTATACA AGACACAATG GAGCTCCTGG AGTGTCTGGG ACAGATACGC
    TCCTTGCTCA TCGTTCAGAT GGGACCAGAG GAAGAGAGGC TGATGATCCA GAGTATCGGA
    AATATTATGA GTAACAGGGT ATTTTACCAG CACCCCAACT TGATGAGAGC TCTGGGAATG
    CATGAGACAG TCATGGAGGT TATGGTGAAC GTACTCGGAG GCGGAGATTC CAAGGAGATC
    AGATTCCCTC GAATGGTTAC CAACTGCTGT CGTTTCCTGT GTTATTTCTG TCGTATCAGC
    AGACAGAATC AGCGCTCGAT GTTTGATCAT CTGGGCTACC TCCTGCAGAA CAGTGGCATT
    GGGCTGGGAA TGCGTGGCTC CACTCCTCTT GATGTTGCTG CTGCTTCCTG TATCGATAAT
    AATGAGCTTG CCCTGGCTCT CCAAGAGCAA GACCTGGAGA TGGTAGTAAA GTACCTGGCA
    GGCTGTGGTC TGCAGAGCTG CCCTATGCTT TTGTCAAAGG GTTACCCGGA TATTGGATGG
    AACCCAGTTG GAGGAGAAAA GTATCTGGAT TTCCTGCGCT TTGCTGTGTT TGTGAATGGA
    GAGAGTGTGG AGGAGAACGC TAATGTGGTG GTCCGGCTGC TGATCAGGAG GCCTGAGTGC
    TTTGGTCCTG CACTGAGAGG AGAAGGGGGC AATGGTCTGC TGGCTGCCAT TGAAGAAGCC
    ATCAAGATCT CAGAGGACCC TGCAAGAGAT GGACCCACTG TTAAAAAAGA CAGACGCTTC
    CCGATGTTTG GAGGTGAAGA GCAGCATGAG GAGAACAGAG TGCACCTAGG AAATGCCATC
    ATGTCATTTT ACTCAGCTCT TATTGATCTG CTGGGTCGCT GTGCTCCTGA GATGCATTTG
    ATCCAGGCTG GTAAAGGTGA AGCTTTGAGA ATCAGGGCTA TTCTTCGGTC GCTTGTGCCC
    ATTGAGGATC TGGTCGGTGT GATCAGCTTG TCAGTCCAGA TTCCTGCTTT TGGAAAAGAT
    AACAGCATTA TTGAGCCGAA GATGTCTGCT AGTTTTGTTC CTGATCATAA AGCACCAATG
    GTCCTGTTCC TGGACAGAGT TTATGGCATC GACAACCAGG ACTTCCTATT GCATGTACTA
    GAAGTGGGTT TCCTGCCTGA TATGAGGGCG GCAGCCTCCT TAGACACAGC TGCATTCAGC
    ACTACAGAAA TGGCCTTGGC TCTGAACCGT TACCTGTGCT CCGCCGTGCT TCCTCTCATC
    ACTAAATGTG CTCCTTTGTT CGCCGGGACG GACCACCGAG CCATCATGAT CGACTCCATG
    CTTCACACCA TCTACAGGCT GTCTCGAGGG CGCTCGCTAA CGAAAGCACA AAGAGATGTT
    ATTGAGGAAT GCCTCATGTC TTTGTGCAGG TATCTGCGCC CTTCCATGCT CCAGCACCTT
    CTCAGGCGGC TAGTATTTGA TGTGCCTATT CTGAATGAGT ATGCCAAAAT GCCCCTTAAG
    CTGCTTACCA ATCATTATGA GCGTTGTTGG AAATATTATT GCTTGCCTAA TGGCTGGGCT
    AACTTTGGCG TATCATCAGA AGAGGAGCTT CATCTGACCC GTAAACTCTT TTGGGGGATC
    TTTGAATCTC TGGCTCACAA GAAATTCGAT GCAGAGCTGT TCAAAATCGC CATGCCTTGT
    ATATGTGCGA TCGCTGGTGC AATTCCTCCT GATTATGTGG ACGCCAGCTA CTCCTCCAAA
    ACCGAAAAAA AGGCTTCTGT TGATGCTGAA GGGAACTTTG ACCCCAAACC TGTTGATACC
    ACTAACACTA TAATTCCCGA GAAGTTGGAC GGGTTCATCA ACAGATATGC AGAATATACG
    CACGACAGAT GGGCTTTTGA AAAAATTCAA AACAACTGGA CATTTGGTGA GGTGATGGAT
    GAAAATGCCA AGACTCATCC CATGCTCAGA CCTTACAAAA CATTCTCTGA AAAGGATAAA
    GAAATCTACC GCTGGCCTAT TAAAGAGTCC ATCAAGGCCA TGATTGCCTG GGAGTGGACA
    CTGGACAAAG CCAGAGATGG AGAGGATGAG AAAGTTGAGA AGAAAACAGC CCGTAAGATC
    TCACAGACTG CACAGGCAAC CTATGACCCC AGTCATGGCT ATAGCCCCCA ACCTATTGAT
    ATCTCAGGCA TGACACTGTC CAGAGAGCTA CAGTCCATGG CAGAGCAGCT CGCAGAAAAC
    TACCACAACA CCTGGGGCCG TAAGAAGAAA ATGGAATTAC AAGCAAAAGG AGGAGGTACA
    CACCCTTTGC TTGTGCCTTA TGACACACTG ACGGCTAAAG AAAAGGCACG AGATAGAGAG
    AAAGCGTATG AGCTACTCAA GTTTCTGCAG CTCAATGGAT ATGCTGTGAC AAGAGGGCTC
    AAAGACATGG AAAGTGACAT TTCATCTATC GAGAAGCGTT TTGCGTATGG CTTCCTACAG
    AAGCTTCTGA AGTGGATGGA CATTGCTCAG GAGTTCATAG CACATCTTGA GGCGGTTGTG
    AGCAGCGGTC GTGTGGAAAA GTCACCACAT GAGCAAGAAA TCAAGTTCTT TGCCAAGATT
    CTTTTGCCTT TGATAAATCA GTATTTTAAA AACCACTGCC TTTACTTCCT CTCCACACCG
    GCCAAAATCC TAGGCAGCGG CGGACACGCC TCAAACAAGG AAAAGGAGAT GATTGCAAGC
    ATTTTCTGTA AGATGTCTGC CCTGGTGCGA CACAGAGTGT TTCTCTTTGG CACGGATGCG
    CCTGCTATTG TCAACTGCCT TCATATCCTC GCACGTTCGC TTGATGCCAG GACGGTGATG
    AAATCCGGGC CTGAGATTGT GAAAGCAGGA CTGCGTTTGT TCTTTGAGAG TGCAGCTGAT
    GACATTGAAA AGATGGTGGA GAATCTCAAA CTTGGCAAAG TGTCCAAAGG CAATCAGCCA
    GTGAAAGGTG TGTCCCAGAA TATAAACTAC ACCACAACGG CTCTGCTTCC TGTCCTCACA
    TCACTGTTTG ACCACATCGC TCAACACCAA TTCGGAGATG ATGTTATCCT GGATGATTTG
    CAGATGTCCT GCTACCGAAT CATGTGTAGC ATCTACTCTC TTGGGACTGT AAAAACTCCA
    CATGCAGAGA GGCATAGACC AGCCCTTGGA GAATGTCTCG CTCATCTCGC TGCAGCAATG
    CCTGTTGCCT ACCTGGAGCC GCATCTTAAT GAATACAACG CCTTTTCAGT CTACACTACC
    AAGACACCGA GAGAAAGAGC TATCCTTGGC CTTCCAAACC AAGTTCAAGA GTTGTGTTTG
    GACATTCCTG AGCTTGACGT TTTACTGAAG GAGATCGGAG ACCTGGCTGA ATCAGGAGCT
    CGATACACTG AAATGCCACA TGTGATTGAG GTCACTCTGC CCATGCTGTG TAACTATCTG
    CCCCGCTGGT GGGAGAGAGG ACTGGAGAAC TTCCCGGATC TAGAGGGTAA GCTGTGCACC
    GACGTCACCT CCGAATCCCT CAACCAGCTT CTGGGAAGCA TCATGAAAAT CGTGGTCAAC
    AACTTGGGAA TCGACGAAGC CTCCTGGATG AAGAGACTTG CAGTGTTCTC CCAACCCATT
    GTGAGCCGAG CCAAACCAGA GATGCTCAAA TCCCACTTCA TCCCCACCAT GGAGAAGCTG
    AAGAAGCGAA CCTCTAAAGT TGTAGCCGAG GAGGAGCATC TGCGTTTGGA GGGAAAGTCT
    GAGGGAGATG AGGAAGAGGG AACTATCAGA GACGAGTTTG CAGTTCTCTG TAGAGACCTC
    TATGCTCTAT ATCCTCTTCT CATCCGCTAT GTGGATAACA ACAGAGCAAG ATGGCTGACG
    TTTCGAGACC CAGATGCTGA GGAGTTGTTC CGCATGGTGG GAGAGGTCTT CATCTTCTGG
    TCAAAATCTC ACAATTTCAA GCGAGAGGAG CAAAACTTTG TGGTGATGAA CGAAATCAAC
    AATATGTCCT TTCTCACTGC TGACAGTAAG AGCAAGATGA GCAAGGCCGG GGACTCTGAG
    GCTGGAGGCT CGGATGTGGA GCGTACCAAG AAAAAGAGAC GGGGGGATCG ATACTCTGTG
    CAAACGTCCC TCATTGTGGC CGCACTGAAG AAAATGCTTC CCATTGGACT TAACATGTGT
    TCTCCTGCAG ACCAGGAGCT GATTAATCTG GCAAAGACAC GTTATTCTCT GAAAGACACA
    GATGAGGAGG TCAGAGAGTT CTTGCAAAAT AACCTGCACC TTCAGGGAAA GGTTGATAAC
    CCATCTATGA GGTGGCAGAT GGCACTGTAT AAGGAAATGG CAGGAAAGGC TGAAGATGCA
    GATGCCCCAG AGAATGTGGT CAAGAGGGTT CAGGAGGTCT CTGCGGTCCT TTACCACATT
    GAAGTGACGG AGCACCCTTT CAAGTCCAAA AAGATGGTTT GGCACAAACT GCTGTCGAAG
    CAGCGGCGTA GAGCGGTGGT GGCCTGTTTC AGGATGACTC CACTTTACAA CTTGCCCAGA
    CACAGAGCAT CTAATATGTT CCTGGATGGG TACAAGCGCA ACTGGATAAG AAATGAAGGC
    TATTCCTTTG AGGACAGAAT GATTGATGAC CTGTCTAAAG CATTGGAGGA GGAAGAAGAG
    GAGGAAGAGG AAAAGGAGAC AAAGCCAGAC CCTCTTCATC AGCTCATCCT TCACTTTAGC
    CGCACAGCAC TCACTGAGAA AAGTAAACTG GATATAGATC ACTTATACAT GGCATATGCT
    GATATCATGG CTAAGAGTTG CCACATGGAT GAAGAGGATG AGGGTGGAGA GGAGGAGAAT
    GAGGGTGGAG AGGAGGAGAT GTCCTTTGAG GAAAAGGAAA TGGAAAAGCA AAGACTGCTC
    TACCAGCAGT CGCGTCTACA CAACCGAGGG GCTGCTGAAA TGGTCCTGCA GATGATCAGT
    GCCTGCAAGG GTGAGACTGG TTGTATGGTC TCTTCCACCC TAAAACTTGG CATTTCTATT
    TTAAATGGCG GGAACTGTGA AGTTCAGCAG AAAATGCTGG ACTACCTGAA AGACAAGAAA
    GATGTGGGTT TCTTTCTCAG TGTTCAGGCT TTAATGCAGA CCTGCAGTGT TTTGGACCTG
    AATGCCTTTG AGAGGCAGAA TAAAGCTGAG GGTCTAGGTA TGGTGTCAGA AGAGGGTACA
    AACATGAAGC TAGAACGGGG TGAGAAGGTC ATGGCTGATG ACGAGTTCAC TTGCGACCTC
    TTCCGCTTTC TCCAGCTCCT CTGTGAGGGT CACAACAATG ACTTCCAAAA TTACCTCAGA
    ACACAGACAG GCAGTACCAC CACCATCAAT GTCATTATCT GTACAGTGGA TTACCTGCTT
    AGACTACAGG AGTCCATCAG TGATTTCTAC TGGTATTACT CTGGAAAAGA CATCATAGAT
    GAACCAGGAA AGCGAAACTT CTCTAAAGCT ATGACTGTGG CCAAACAGGT TTTCAACAGT
    CTTACTGAGT ATATCCAGGG TCCCTGCACA GGAAACCAGC AGTCCCTGGC TCACAGTCGC
    TTATGGGATG CTGTGGTGGG CTTTCTGCAC GTGTTTGCTC ATATGATGAT GAAACTGGCT
    CAGGGAAGAG ATTCCAGTCA GATTGGTCTT CTGAAAGAGT TGTTGGACCT TCAGAAAGAC
    ATGGTGGTCA TGTTATTGTC CTTGTTGGAG GGTAATGTTG TAAATGGCAC CATTGCTCGC
    CAGATGGTGG ACATGCTAGT GGAATCCTCC AGCAATGTAG AAATGATCCT TAAGTTCTTC
    GACATGTTCC TCAAGCTGAA GGACATTGTT GCGTCTGACG CGTTTCGTGA CTACGTGACC
    GACCCTAGAG GTTTGATATC CAAGAAAGAC TTTCAGAAAT CCATGGACAG CCAGAAACAG
    TACACACCTT CAGAGATCCA ATTCCTATTG TCATGCTCTG AGGCTGATGA GAATGAGATG
    ATCAATTACG AGGAGTTTGC AAGTCGCTTT CAAGAGCCGG CCAAAGACAT TGGTTTTAAC
    ATAGCGGTGC TGCTAACCAA CCTCTCCGAA CATGTTCCTC ACGATACAAG ACTGCAGAAC
    TTCCTCGAAC AAGCCGAGAG CGTCCTGAAC TATTTCCGCC CATTCTTGGG TCGCATCGAG
    ATCATGGGCG CCAGCAGGAA AATCGAGAGA ATCTACTTCG AGATCAGCGA GGTGAATCGC
    AAGCAGTGGG AGATGCCTCA AGTCAAAGAA TCAAAGAGAC AGTTCATATT CGATGTAGTC
    AATGAAGGCG GCGAATCGGA GAAGATGGAG CTTTTCGTCA ACTTCTGCGA GGACACCATA
    TTCGAGATGA ACATTGCGTC GCAGATTTCA GAACAGGACG AGGAGGAGAA AGAAGAGGAT
    GATATTGAGG AAGCTGAAGG AGGAGATGCT GGTGATGAAG AGGGGGGTGG CGAGGAGGGA
    CAACCAGAAT CAAGCTCTGC ATTTGCCGAT TTCATTCAAA GCATCATGAA TTTTCTGGGT
    ATGTTCACCT TCAGGAATAT TCGCAGAAAG TACCGCAGAC TCCGCAAGAT GACCATTAAG
    GAGATCGTGG TAGGGCTTGC TACCTTCATG TGGACGATTC TCTTGGGCAT CCTCCATTTC
    ATTTACAGTG TTTGTAAAGG CTTCTTCCTC CTCATCTGGC ACACGCTGTT TGGAGGAGGT
    TTAGTTGAAG GGGCCAAAAA CATCACAGTA ACTGAGTTAC TAGCCAGCAT GCCAGATCCC
    ACACAGGACG AGGTGCATGG GGACTTACCT GGAGAACCGA GGGGAGGAGA GGAACAGGAG
    ACTGGAGGGA TAATGGACTC GATGGATGCT GGAGGGGGAG AGGAAGAGGA TGAGGATATC
    CAGGAGAAAG ATGGTGGAAA GATTCCTGGT ATAGATACAC CTGGTGGACT TGGAGATATG
    GGTGATACAA CTCCTGTAGA ACCGCCGACT CCCGAGGGTA CCCCTTTGTT GAAGAGGAAA
    GCGCAACCAG ATGAAGCAGG AGCTGAACAG CCTCCTGCTA TTGAAGAGCC TCCTCCTGAG
    CCTGAGAAAG CAGACATTGA GAATGGTGAG AAGGCTGAGA AGGAAGTGGA GGTCAAGCCT
    GAGCCAATAC CAGAAGAACC GAAACCTCAG AGAGCCACCA AAGCCAAGAA GGAGAAGAAA
    TCAGTCAAGG GTGCAGGATT TGAGCTCTGG AATGAGCTGG ATGTGCAAAG AAATAAATTC
    ATGAACTGCT TGTCTCGTAA CTTTTACAAC ATTCGTTTCC TGGCTCTGTT CCTTGCCTTT
    GCCTTGAACT TCATTCTTCT CTTCTACAAG GTATCTGATA CTCCCCCCAG TTCTGATGAA
    TTTGAAGGTT CTGGCATCTT TGAAGGCTCT GGGCTGTTTG AAGGCTCTGC GGAGGATTTT
    GAGGGCTCTG GTGGAGAGGA TGGAGGTGAT GATGAGGATG AAGAAGGACC TGTGTATTAT
    TTTCTGGAAG AAAGCACGGG ATACATGCAG CCCACTCTGT CCTTCCTGGC CATTTTGCAC
    ACCGTCATTG CTTTCATATC CATCATTGGG TACAACTGCC TCAAGATTCC ACTGGTCATC
    TTTAAGAGAG AGAAAGAGTT GGCGAGAAAG CTGGAGTTTG ATGGCCTTTA CATCACTGAA
    CAGCCGGCAG ATGATGACAT TAAAGGCCAG TGGGACAGAC TGGTGTTGAA CACTCCCTCT
    TTTCCCACCA ATTACTGGGA CAAATTTGTG AAGCGAAAGG TTCTGGATAA ATATGGAGAT
    ATTTATGGAA GAGAGAGAAT CGCAGAGCTG CTGGGTATGG ATCTGGCATC TCTGGATGTC
    AGCAAACAAC AGACAGACAA GAAACCAGAA GAGCCAGACA ACTCTATGCT TGCATGGTTC
    ACTTCCATTG ACATCAAGTA TCAGATCTGG AAATTTGGAG TTGTGTTCAC AGACAATACT
    TTCCTCTACC TGGTGTGGTA CACAGTGATG TCCCTTCTTG GACATTACAA CAACTTTTTC
    TTTGCTTGTC ACCTGCTGGA CATCGCAATG GGTGTTAAGA CCCTGCGTAC AATTCTTTCC
    TCCGTCACAC ATAATGGAAA ACAGCTGATG ATGACTGTGG GTTTGCTGGC TGTGGTTGTG
    TACCTCTACA CTGTGGTGGC CTTCAACTTC TTCAGGAAGT TCTACAATAA GAGCGAGGAT
    GAGGATGAGC CGGATATGAA GTGTGACGAC ATGATGACGT GTTATTTGTT CCACATGTAT
    GTGGGTGTAC GGGCAGGTGG AGGTATAGGA GATGAGATCG AGGACCCTGC TGGAGATGAG
    TATGAGCTCT ATCGGGTGGT CTTCGACATC ACCTTCTTCT TCTTTGTCAT CGTCATCCTG
    CTGGCCATCA TTCAGGGTCT GATCATTGAT GCCTTTGGTG AACTGAGAGA CCAGCAAGAG
    CAAGTGAAAG AGGACATGGA GACCAAATGC TTTATATGTG GCATTGGGAG CGACTACTTT
    GATACAACGC CCCATGGCTT TGAGACCCAT ACGCTTGAAG AACACAACCT GGCCAATTAC
    ATGTTTTTCC TGATGTACTT AATCAACAAG GATGAAACAG AGCACACTGG CCAGGAATCA
    TACGTGTGGA AGATGTACCA GGAGAGATGC TGGGATTTCT TCCCTGCAGG AGACTGCTTC
    AGAAAGCAAT ATGAAGACCA GCTTGGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa47274
    Clone ID Related Accession (Same CDS sequence) XM_009305502.3 , XM_009305502.2
    Accession Version XM_009305502.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 15192bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product ryanodine receptor 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009305502.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    13261
    13321
    13381
    13441
    13501
    13561
    13621
    13681
    13741
    13801
    13861
    13921
    13981
    14041
    14101
    14161
    14221
    14281
    14341
    14401
    14461
    14521
    14581
    14641
    14701
    14761
    14821
    14881
    14941
    15001
    15061
    15121
    15181
    ATGGCAGAAG GAGCTGAGGG AGAAGAGGAG ATCCAATTCC TAAGAACAGA TGACCAGGTG 
    GTGCTACAGT GTACAGCTAC TATCCTGAAG GAGCAGATTA AACTCTGTCT GTCCTGTGAG
    GGTTTTGGGA ATCGCCTGTG TTTTTTAGAA ACCACATCTA ATGCTCAGAA TGTTCCTCCT
    GATCTGGCAA TATGCTGCTT TATCCTGGAG CAGTCTTTAT CTGTGCGGGC CCTGCAGGAG
    ATGCTGGCTA ACACTACTGA GCTTAATGAG GCAGTCGATT TGGATAAATG GTCTTCACAA
    GGAGGAGGCC ACCGCACGTT GTTGTATGGC CACGCTATCC TACTCAGACA TACTCACTCT
    GGCATGTACC TTAGTTGCTT GACCACATCA AGATCCCTCA CAGACAAACT GGCCTTTGAT
    GTGGGCTTGC AGGAGGACTC CACAGGTGAG GCCTGTTGGT GGACCATTCA CCCTGCATCC
    AAGCAGAGGT CAGAGGGTGA GAAGGTCAGA GTGGGCGATG ACATTATTCT GGTCAGCGTG
    TCCTCTGAGA GATATCTGCA CCTCTCCTAT GCCAGCGGAG ACCTGATGGT GGACGCTTCT
    TTCATGCAGA CCCTTTGGAA CATGAACCCA GTCTGTTCAG GCTGTGAGCT GGCAGAGGGG
    TTTCTGACTG GAGGGCATGT TCTTCGTCTA TTTCACGGGC ACATGGATGA GTGTTTAACA
    ATTCCTGGCG CGGAGCAGGG CGACGATCAG AGACGAAATG CTCACTATGA AGGTGGAGCC
    GTGTGCAGCC ATGCTAGATC TCTCTGGAGA CTGGAGCCTC TTCGCATTGG ATGGAGTGGT
    AGCCACATGA AGTGGGGTCA GTCATTCAGG GTGCGCCACA TCACAACAGG CAGGTATCTG
    TGTCTGGACG AAGAGAAAGG ACTCCTGATT GTGGACCCAG AAAAGGCAAA TACGAAGCAG
    TCAGCTTTCT GCTTTCGTGC ATCAAAGGAG AAGACAGAAG TAGCTCAGAA ACGTGATGTG
    GAGGGAATGG GGATCCCAGA GATCAAGTAT GGCGAGTCCA TGTGCTTTGT GCAGCACGTC
    GCTACAAGTC TGTGGCTCAC ATATGCCTCG GTGGATGCCA AGTCTGCTCG GCTGGGACCA
    CTGAAGAGGA AGGCTATCCT GCATCAGGAG GGTCACATGG ATGATGCTTT GACTGTGGCT
    CGCTCTCAGA CAGCTGAATC GCAGGCTGCC AGGATGATCT ACAACACGGC AGGACTTTTC
    ACTCAGTTCA TTAAGGGTTT GGACTCTCTA AGTGGGAAGA GTAAATCTCC AGGACCTTCC
    CCTTCTCTGC CTCTAGACGC CATAGTTCTG TCATTGCAGG ATCTCATCTT CTATTTCCGA
    CCTCCAGAAG AGGAGCTGGA ACATGAGGAG AAGCAGACCA AACTGCGCTC CTTAAGAAAC
    CGCCAGAATC TCTTCCAAGA GGAGGGCATG ATCACTCTTG TATTGGACTG TGTTGATCGG
    TTGAATGTCT ACAACACTTC AGCCCACTTC TCAGAATTTG CTGGAGAGGA CGCAGCCGAG
    TCGTGGAAAG AGATCGTCAA CTTATTTTAT GAGCTGCTGG CATCTCTGAT CAGAGGAAAC
    AGGGCAAACT GTGCTTTGTT CTGTGATAAT CTTGACTGGT TGGTCAGCAA ACTGGATCGT
    CTGGAGGCAT CCTCGGGTAT TCTGGAGGTG CTTTATTGTA TCCTAATTGA AAGCCCTGAG
    GTGCTTAACA TCATCCAGGA AAACCACATC AAATCTATCA TTGCACTTTT GGACAAACAT
    GGACGCAATC ACAAGGTTTT AGATGTTCTC TGCTCATTAT GCGTGTGTAA TGGTGTTGCT
    GTGAGGTCCA ATCAGAATCT GATCACTGAA AACCTGCTTC CCGGACGGGA TCTTCTCCTT
    CAGACCAACA TCATTAATTA TGTTACCAGT ATGAGACCCA ACATTTTTCT GGGCACGTGT
    GAAGGCTCGA CTCAGTATAA GAAGTGGTAC TATGAGTTGA TCGTTGACCA TGTGGAGTCT
    TTCCTTACCG CTCATCCATT TCACCTTCGT GTTGGCTGGG CTTTGACGGA GGGCTACAGC
    CCGTATCCAG GAGGTGGAGA AGGATGGGGA GGCAATGGAG TTGGAGATGA TCTACACTCG
    TATGGTTTCG ATGGGTTGAA TCTGTGGACA GGTCGTGTAC CACGTCAGGT GACGTCACCC
    AGTCAACACA TTCTCTCGGC TGAGGATGTG GTGAGCTGCT GTTTAGATCT AAGCGTTCCC
    AGCATCTCCT TTCGTATTAA TGGACATCCC GTTCAAGGCA TGTTCGAGAA CTTCAATCTG
    GATGGTCTTT TCTTCCCCGT GGTCAGCTTC TCAGCGGGGA TAAAAGTTCG TTTTCTCCTT
    GGCGGACGAC AAGGAGACTT CAAATTCCTC CCTCCTCCAG GTTATGCCCC GTGCTATGAG
    GCCGTTCTGC CCAAAGACAG ACTGCGGATT GAACCCATTA AAGAGTACAA GCACGACAAC
    GACGGTGTCC GAAACCTGCT GGGACCGACA CAGTCTCTTT CCCACACCGC CTTCACTCCC
    TGTCCGGTGG ACACTGTGCA GATTGTGCTT CCTCCACATC TAGAGCGCAT CAGAGAGAAA
    CTGGCCGAAA ATAGCCATGA GCTGTGGGCC GTCACTCGAA TTGAACAGGG TTGGACATAT
    GGACTATTTC GTGATGACAA CAAGAAGCTG CATCCCTGTC TAGTGGACTT CCAGAGCCTC
    CCGGATCCTG AGAAGAGCTA CAACCTGGCC ATGTCTGGAG AGACGCTGAA GACTCTTCTG
    GCTTTAGGGT GTCATGTAGG CATGGGTGAT GAAAAAGCAG AGGAGAACCT GAAGAAGATC
    AAACTTCCTA AAACGTATAT GCAGTGTAAC GGATACAAAC CTGCTCCACT GGATCTTAAC
    CATGTGAAGC TCACACCGAA TCAGAACACA CTTGTGGAAA GGCTGGCTGA AAATGGACAC
    AACGTCTGGG CTCGAGACCG TGTCCGCCAA GGGTGGACTT ACAGCATCAT TCAGGATATT
    GTGAACAAGC GCAACCCTCG TCTCGTGCCT TACATTCTGC TTGATGAAAA GACAAAAAAG
    ACCAATCGTG ACACTGTGTG TGCCGCTGTA CGGACTCTGA TTGGCTATGG CTACAATATT
    GAACCCCCTG ACCAGGAGAG CAGTGGTCAT GGGGGTGGCA ATGGCCGGAG TGATAAGATC
    CGTGTGTTCA GGGCAGAGAA ATCCTACGCA GTGACGCAGG GTAAATGGTA CTTTGAGTTC
    GAGGCAGTAA CAGTGGGAGA GATGAGAGTT GGGTGGGCAA GACCAAGCGT CCATGCAGAC
    AGAGAGCTGG GATCAGATGA CCTGGCCTAC GTGTTCAATG GCTTTAAGTC TCAGCGCTGG
    CACATTGGAA ATGAGCCGTT TGGCCGACAG TGGCAGTCTG GTGATGTCGT TGGCTGCATG
    ATAGACCTGA CGGAGCAGAA CATCATGTTT ACTTTGAATG GAGAGATGCT CATCAGTGAC
    TCTGGATCTG AGATGGCATT TAAAGACATT GAGATCGGAG AAGGCTTCAT CCCTGTATGC
    AGTCTGGGTA TGTCCCAGGT GGGTCGGATC AACCTTGGTC AAAATGTGAG CAGCCTGCGG
    TACTTTACAA TCTGTGGACT GCAGGAAGGT TTCGAGCCAT TTGCTATCAA CATGAAGAGA
    GATATTACAA TGTGGTTCAG CAAGAGCTTG CCCCAGTTTG TGCCCGTTCC AACAGATCAT
    CCTCATATTG AGGTGTCCCG TGTGGATGGA ACGGTGGAGA GCGCTCCCTG TCTGAAACTG
    ACTCACAGGA CATTCGGTTC TCAGAATGCC AACACAGACC TGTTGTTCTT CAGACTCAGT
    ATGCCGATTG AATTCCACGA AACCTTCAAG GTCCCGGTGG GAGCTTCACC CCTCACACGA
    ACCCTCACTA TCCCTGAGGA TGAGACTCTT GAGGTGGATC CCGAATCTGA ATTCGAGCTC
    CTGAAGAAGT CAGCCACTCG TAAGGAGCAA GATGAGAAAG AAAAAGAGCC GTCCGTCCCG
    AAAGAGCTGC CTGGAAGCAA AGAAAGCGAG AATGAGAAGG ACACCACCAC AGAGAAGAGC
    AAAAAGAGAG GATTTTTTTC CAAGGCGAAG AAGGCAGCGT TTATTGCCCC ACCACCAGTG
    GTTCCAACAA TGCCTCGTCT AATGGAGGAT GTTGTCCCAG ATGATCGAGA CGATGTTGAA
    ATTATCTCAA ACACAACAAC TTATTATTAT TCAGTCCGGG TGTTCGCCGG ACAGGAGCCC
    AGTGGAGTAT GGGTAGGCTG GGTGACTCCT GATTACCACT TGTACGACCT TCACTTTGAT
    CTTTGTAAAG TCAGAAACGT AACTGTTACA GTTGGAGACG ATAAAGGCAA TATTCATGAC
    AGTGTGAAAC GCAGTAATTG CTACATGGTG TGGGGTGGAG AGTTTAGCAG CTCACAGCAA
    ACCCGCATCA GTCAGGAGGA CTTTGTGATC GGTTGTTTGG TGGACCTGGA CACAGGACTG
    ATGACCTTTA CAGCCAATGG GAAAGAGATC AATACGTTCT ATCAGGTTGA ACCTAACACT
    AAGCTTTTCC CTGCTGTGTT TTGTTTGCCA TCAAGTCAGA ACATGTTACA AGTGGAACTC
    GGCAAACTGA AGAACATTAT GCCCATCTCA GCAGCCATGT TTCGCAGCGA CTGTAAGAAC
    CCGGTCCCTC AATGCCCTCC CAGGTTAGAT CTTCAGATGC TGACCCCTGT CATATGGAGC
    CGGATGCCTA ATCACTTTTT GTCGCCTGAA ACTGGCCGCG TGAGTGAGAG GCAGGGCTGG
    AAAGTGCAAT GCATGGAACC TCTCGTTATG ATGGCACTGC ACATCCCTGA AGAGAACAGG
    TGTATTGACA TCCTGGAACT GTCAGAACGT ACCGACCTGA TGAAGTTCCA TTACCACACA
    CTGAAACTGT ATGGCTCGGT TTGCGCTCTG GGGAACAATC GTGTCGCACA CGCCCTCTGC
    AGCCACGTGG ACGAATCGCA GCTCTTTTAC GCCATCGAAA ACACGTACCT GCCTGGCCCG
    ATCCGCAGTG GCTACTACGA CCTGCTCATC TCCATGCACC TAGAGTCTGC CAAACGCAAC
    CGACTCATGA CCAATAAGGA GTTCATTGTG CCCATGACGG ACGAGACTCG AAGCATCACC
    CTTTATTCGG ATGCTGAGAA GGCACATGCG TTACCTGGTG TCGGACTTAC CACATGTCTA
    CGCCCAAAAC TTCACTTTGC TCCAACTGGA TTTGTGGGCA CTAATGCTGA CCTTTACACC
    CTGAGCCCCA TGTTACCTCT TCAGATGCTT AAAGATCATG CCCTCAGCAT GCTTACAGAA
    GCTGTGCAGG ACGGTGGCCA AGCCATTCGA GATCCTGTAG GGGGCAGCGT TGAGTACCAC
    TTTGTGCCCA TCCTGAAGCT CATCAGCACC CTTCTCATTA TGGGTGTATT TGATGACGAC
    GATGTCAAGC ACATCCTGAA ACTGATCGAA CCATCCGTCT TCAGCAGCAA CAGTGAGACT
    CCAGCTGAAG AAACTGGAGA TCCTGAACAG CAGCTGGTCT GCAAAGAGGA AGGAGCGGAG
    GTAAAAGAGC AAGAGGAACC AGCAGACGGT GGAAATGATG GAGAGCTGCT GGATGAAGGG
    ATGGGAGAGG AAGAAGAGCT TGAGGAAGAG GAGGAGGAGG AAGATGAAGA GTATGAGGAG
    GAGGTGAAAA AAGAGGAGGA TGAACATGCT GCTGAAAAAA CAGACAGAGA GAAAACTACA
    GATGGAGAAA CTGAGAAGGA AACTGGTGCA GCAGAGGCTG AAGTAAGGGA GGAGGAGGAT
    GTTGGAGAAG GACTGCTGCA CATGAAGCTT CCAGAGTCTG TCAAACTCCA GATGTGTACT
    CTCCTGCAAT ATTTCTGTGA CTGTGAGCTG CGTCACAGAG TGGAAGCCAT CATCGCTTTC
    TCAGACAAGT TTGTTAACCA AGTGCAGACC AATCAGAGAC AGCGATATAA TGAGCTCATG
    CAAGCTTTTA CAATGAGTGC AGCAGAAACG GCCCGTAAGA CCCGCGAATT CCGCTCCCCT
    CCACAGGAAC AGGTGAACAT GTTGATGAAC TTTAAGAACT GTCCAGAGGA AGAAGATTGC
    CCCGTTCCAG AGGATGTCCG GAATGGCCTC CTGACATTTC ACCATGACCT TTTAGCTCAC
    TGTGGTGTGC ACATTGAAGA GGAGGAACAG GAAGAAGAGG TGGACACTTC TCTCAGTGGG
    CGCCTACGCA GACTGGTGGA AAAAGTTAAA AGCTTGCGAA AAAAGCCAGA AGCTGAGCCT
    GAGCCTGAAG AAGATAAAAA ACCAAGTACT CTGCAGGAGC TGATCTCCCA CACCATGATC
    TACTGGGCTC AAGAGTCTTT CATCCAGAAC CCTGAGCTGG TGCGCCTCAT GTTTAGTCTG
    CTACACCGGC AGTATGATGC TTTGGGTGAG CTGATTCGAG CTTTGCCCAA AGCCTATATC
    ATTAATGCTG TTTCTATACA AGACACAATG GAGCTCCTGG AGTGTCTGGG ACAGATACGC
    TCCTTGCTCA TCGTTCAGAT GGGACCAGAG GAAGAGAGGC TGATGATCCA GAGTATCGGA
    AATATTATGA GTAACAGGGT ATTTTACCAG CACCCCAACT TGATGAGAGC TCTGGGAATG
    CATGAGACAG TCATGGAGGT TATGGTGAAC GTACTCGGAG GCGGAGATTC CAAGGAGATC
    AGATTCCCTC GAATGGTTAC CAACTGCTGT CGTTTCCTGT GTTATTTCTG TCGTATCAGC
    AGACAGAATC AGCGCTCGAT GTTTGATCAT CTGGGCTACC TCCTGCAGAA CAGTGGCATT
    GGGCTGGGAA TGCGTGGCTC CACTCCTCTT GATGTTGCTG CTGCTTCCTG TATCGATAAT
    AATGAGCTTG CCCTGGCTCT CCAAGAGCAA GACCTGGAGA TGGTAGTAAA GTACCTGGCA
    GGCTGTGGTC TGCAGAGCTG CCCTATGCTT TTGTCAAAGG GTTACCCGGA TATTGGATGG
    AACCCAGTTG GAGGAGAAAA GTATCTGGAT TTCCTGCGCT TTGCTGTGTT TGTGAATGGA
    GAGAGTGTGG AGGAGAACGC TAATGTGGTG GTCCGGCTGC TGATCAGGAG GCCTGAGTGC
    TTTGGTCCTG CACTGAGAGG AGAAGGGGGC AATGGTCTGC TGGCTGCCAT TGAAGAAGCC
    ATCAAGATCT CAGAGGACCC TGCAAGAGAT GGACCCACTG TTAAAAAAGA CAGACGCTTC
    CCGATGTTTG GAGGTGAAGA GCAGCATGAG GAGAACAGAG TGCACCTAGG AAATGCCATC
    ATGTCATTTT ACTCAGCTCT TATTGATCTG CTGGGTCGCT GTGCTCCTGA GATGCATTTG
    ATCCAGGCTG GTAAAGGTGA AGCTTTGAGA ATCAGGGCTA TTCTTCGGTC GCTTGTGCCC
    ATTGAGGATC TGGTCGGTGT GATCAGCTTG TCAGTCCAGA TTCCTGCTTT TGGAAAAGAT
    AACAGCATTA TTGAGCCGAA GATGTCTGCT AGTTTTGTTC CTGATCATAA AGCACCAATG
    GTCCTGTTCC TGGACAGAGT TTATGGCATC GACAACCAGG ACTTCCTATT GCATGTACTA
    GAAGTGGGTT TCCTGCCTGA TATGAGGGCG GCAGCCTCCT TAGACACAGC TGCATTCAGC
    ACTACAGAAA TGGCCTTGGC TCTGAACCGT TACCTGTGCT CCGCCGTGCT TCCTCTCATC
    ACTAAATGTG CTCCTTTGTT CGCCGGGACG GACCACCGAG CCATCATGAT CGACTCCATG
    CTTCACACCA TCTACAGGCT GTCTCGAGGG CGCTCGCTAA CGAAAGCACA AAGAGATGTT
    ATTGAGGAAT GCCTCATGTC TTTGTGCAGG TATCTGCGCC CTTCCATGCT CCAGCACCTT
    CTCAGGCGGC TAGTATTTGA TGTGCCTATT CTGAATGAGT ATGCCAAAAT GCCCCTTAAG
    CTGCTTACCA ATCATTATGA GCGTTGTTGG AAATATTATT GCTTGCCTAA TGGCTGGGCT
    AACTTTGGCG TATCATCAGA AGAGGAGCTT CATCTGACCC GTAAACTCTT TTGGGGGATC
    TTTGAATCTC TGGCTCACAA GAAATTCGAT GCAGAGCTGT TCAAAATCGC CATGCCTTGT
    ATATGTGCGA TCGCTGGTGC AATTCCTCCT GATTATGTGG ACGCCAGCTA CTCCTCCAAA
    ACCGAAAAAA AGGCTTCTGT TGATGCTGAA GGGAACTTTG ACCCCAAACC TGTTGATACC
    ACTAACACTA TAATTCCCGA GAAGTTGGAC GGGTTCATCA ACAGATATGC AGAATATACG
    CACGACAGAT GGGCTTTTGA AAAAATTCAA AACAACTGGA CATTTGGTGA GGTGATGGAT
    GAAAATGCCA AGACTCATCC CATGCTCAGA CCTTACAAAA CATTCTCTGA AAAGGATAAA
    GAAATCTACC GCTGGCCTAT TAAAGAGTCC ATCAAGGCCA TGATTGCCTG GGAGTGGACA
    CTGGACAAAG CCAGAGATGG AGAGGATGAG AAAGTTGAGA AGAAAACAGC CCGTAAGATC
    TCACAGACTG CACAGGCAAC CTATGACCCC AGTCATGGCT ATAGCCCCCA ACCTATTGAT
    ATCTCAGGCA TGACACTGTC CAGAGAGCTA CAGTCCATGG CAGAGCAGCT CGCAGAAAAC
    TACCACAACA CCTGGGGCCG TAAGAAGAAA ATGGAATTAC AAGCAAAAGG AGGAGGTACA
    CACCCTTTGC TTGTGCCTTA TGACACACTG ACGGCTAAAG AAAAGGCACG AGATAGAGAG
    AAAGCGTATG AGCTACTCAA GTTTCTGCAG CTCAATGGAT ATGCTGTGAC AAGAGGGCTC
    AAAGACATGG AAAGTGACAT TTCATCTATC GAGAAGCGTT TTGCGTATGG CTTCCTACAG
    AAGCTTCTGA AGTGGATGGA CATTGCTCAG GAGTTCATAG CACATCTTGA GGCGGTTGTG
    AGCAGCGGTC GTGTGGAAAA GTCACCACAT GAGCAAGAAA TCAAGTTCTT TGCCAAGATT
    CTTTTGCCTT TGATAAATCA GTATTTTAAA AACCACTGCC TTTACTTCCT CTCCACACCG
    GCCAAAATCC TAGGCAGCGG CGGACACGCC TCAAACAAGG AAAAGGAGAT GATTGCAAGC
    ATTTTCTGTA AGATGTCTGC CCTGGTGCGA CACAGAGTGT TTCTCTTTGG CACGGATGCG
    CCTGCTATTG TCAACTGCCT TCATATCCTC GCACGTTCGC TTGATGCCAG GACGGTGATG
    AAATCCGGGC CTGAGATTGT GAAAGCAGGA CTGCGTTTGT TCTTTGAGAG TGCAGCTGAT
    GACATTGAAA AGATGGTGGA GAATCTCAAA CTTGGCAAAG TGTCCAAAGG CAATCAGCCA
    GTGAAAGGTG TGTCCCAGAA TATAAACTAC ACCACAACGG CTCTGCTTCC TGTCCTCACA
    TCACTGTTTG ACCACATCGC TCAACACCAA TTCGGAGATG ATGTTATCCT GGATGATTTG
    CAGATGTCCT GCTACCGAAT CATGTGTAGC ATCTACTCTC TTGGGACTGT AAAAACTCCA
    CATGCAGAGA GGCATAGACC AGCCCTTGGA GAATGTCTCG CTCATCTCGC TGCAGCAATG
    CCTGTTGCCT ACCTGGAGCC GCATCTTAAT GAATACAACG CCTTTTCAGT CTACACTACC
    AAGACACCGA GAGAAAGAGC TATCCTTGGC CTTCCAAACC AAGTTCAAGA GTTGTGTTTG
    GACATTCCTG AGCTTGACGT TTTACTGAAG GAGATCGGAG ACCTGGCTGA ATCAGGAGCT
    CGATACACTG AAATGCCACA TGTGATTGAG GTCACTCTGC CCATGCTGTG TAACTATCTG
    CCCCGCTGGT GGGAGAGAGG ACTGGAGAAC TTCCCGGATC TAGAGGGTAA GCTGTGCACC
    GACGTCACCT CCGAATCCCT CAACCAGCTT CTGGGAAGCA TCATGAAAAT CGTGGTCAAC
    AACTTGGGAA TCGACGAAGC CTCCTGGATG AAGAGACTTG CAGTGTTCTC CCAACCCATT
    GTGAGCCGAG CCAAACCAGA GATGCTCAAA TCCCACTTCA TCCCCACCAT GGAGAAGCTG
    AAGAAGCGAA CCTCTAAAGT TGTAGCCGAG GAGGAGCATC TGCGTTTGGA GGGAAAGTCT
    GAGGGAGATG AGGAAGAGGG AACTATCAGA GACGAGTTTG CAGTTCTCTG TAGAGACCTC
    TATGCTCTAT ATCCTCTTCT CATCCGCTAT GTGGATAACA ACAGAGCAAG ATGGCTGACG
    TTTCGAGACC CAGATGCTGA GGAGTTGTTC CGCATGGTGG GAGAGGTCTT CATCTTCTGG
    TCAAAATCTC ACAATTTCAA GCGAGAGGAG CAAAACTTTG TGGTGATGAA CGAAATCAAC
    AATATGTCCT TTCTCACTGC TGACAGTAAG AGCAAGATGA GCAAGGCCGG GGACTCTGAG
    GCTGGAGGCT CGGATGTGGA GCGTACCAAG AAAAAGAGAC GGGGGGATCG ATACTCTGTG
    CAAACGTCCC TCATTGTGGC CGCACTGAAG AAAATGCTTC CCATTGGACT TAACATGTGT
    TCTCCTGCAG ACCAGGAGCT GATTAATCTG GCAAAGACAC GTTATTCTCT GAAAGACACA
    GATGAGGAGG TCAGAGAGTT CTTGCAAAAT AACCTGCACC TTCAGGGAAA GGTTGATAAC
    CCATCTATGA GGTGGCAGAT GGCACTGTAT AAGGAAATGG CAGGAAAGGC TGAAGATGCA
    GATGCCCCAG AGAATGTGGT CAAGAGGGTT CAGGAGGTCT CTGCGGTCCT TTACCACATT
    GAAGTGACGG AGCACCCTTT CAAGTCCAAA AAGATGGTTT GGCACAAACT GCTGTCGAAG
    CAGCGGCGTA GAGCGGTGGT GGCCTGTTTC AGGATGACTC CACTTTACAA CTTGCCCAGA
    CACAGAGCAT CTAATATGTT CCTGGATGGG TACAAGCGCA ACTGGATAAG AAATGAAGGC
    TATTCCTTTG AGGACAGAAT GATTGATGAC CTGTCTAAAG CATTGGAGGA GGAAGAAGAG
    GAGGAAGAGG AAAAGGAGAC AAAGCCAGAC CCTCTTCATC AGCTCATCCT TCACTTTAGC
    CGCACAGCAC TCACTGAGAA AAGTAAACTG GATATAGATC ACTTATACAT GGCATATGCT
    GATATCATGG CTAAGAGTTG CCACATGGAT GAAGAGGATG AGGGTGGAGA GGAGGAGAAT
    GAGGGTGGAG AGGAGGAGAT GTCCTTTGAG GAAAAGGAAA TGGAAAAGCA AAGACTGCTC
    TACCAGCAGT CGCGTCTACA CAACCGAGGG GCTGCTGAAA TGGTCCTGCA GATGATCAGT
    GCCTGCAAGG GTGAGACTGG TTGTATGGTC TCTTCCACCC TAAAACTTGG CATTTCTATT
    TTAAATGGCG GGAACTGTGA AGTTCAGCAG AAAATGCTGG ACTACCTGAA AGACAAGAAA
    GATGTGGGTT TCTTTCTCAG TGTTCAGGCT TTAATGCAGA CCTGCAGTGT TTTGGACCTG
    AATGCCTTTG AGAGGCAGAA TAAAGCTGAG GGTCTAGGTA TGGTGTCAGA AGAGGGTACA
    AGTGAGAAGG TCATGGCTGA TGACGAGTTC ACTTGCGACC TCTTCCGCTT TCTCCAGCTC
    CTCTGTGAGG GTCACAACAA TGACTTCCAA AATTACCTCA GAACACAGAC AGGCAGTACC
    ACCACCATCA ATGTCATTAT CTGTACAGTG GATTACCTGC TTAGACTACA GGAGTCCATC
    AGTGATTTCT ACTGGTATTA CTCTGGAAAA GACATCATAG ATGAACCAGG AAAGCGAAAC
    TTCTCTAAAG CTATGACTGT GGCCAAACAG GTTTTCAACA GTCTTACTGA GTATATCCAG
    GGTCCCTGCA CAGGAAACCA GCAGTCCCTG GCTCACAGTC GCTTATGGGA TGCTGTGGTG
    GGCTTTCTGC ACGTGTTTGC TCATATGATG ATGAAACTGG CTCAGGGAAG AGATTCCAGT
    CAGATTGGTC TTCTGAAAGA GTTGTTGGAC CTTCAGAAAG ACATGGTGGT CATGTTATTG
    TCCTTGTTGG AGGGTAATGT TGTAAATGGC ACCATTGCTC GCCAGATGGT GGACATGCTA
    GTGGAATCCT CCAGCAATGT AGAAATGATC CTTAAGTTCT TCGACATGTT CCTCAAGCTG
    AAGGACATTG TTGCGTCTGA CGCGTTTCGT GACTACGTGA CCGACCCTAG AGGTTTGATA
    TCCAAGAAAG ACTTTCAGAA ATCCATGGAC AGCCAGAAAC AGTACACACC TTCAGAGATC
    CAATTCCTAT TGTCATGCTC TGAGGCTGAT GAGAATGAGA TGATCAATTA CGAGGAGTTT
    GCAAGTCGCT TTCAAGAGCC GGCCAAAGAC ATTGGTTTTA ACATAGCGGT GCTGCTAACC
    AACCTCTCCG AACATGTTCC TCACGATACA AGACTGCAGA ACTTCCTCGA ACAAGCCGAG
    AGCGTCCTGA ACTATTTCCG CCCATTCTTG GGTCGCATCG AGATCATGGG CGCCAGCAGG
    AAAATCGAGA GAATCTACTT CGAGATCAGC GAGGTGAATC GCAAGCAGTG GGAGATGCCT
    CAAGTCAAAG AATCAAAGAG ACAGTTCATA TTCGATGTAG TCAATGAAGG CGGCGAATCG
    GAGAAGATGG AGCTTTTCGT CAACTTCTGC GAGGACACCA TATTCGAGAT GAACATTGCG
    TCGCAGATTT CAGAACAGGA CGAGGAGGAG AAAGAAGAGG ATGATATTGA GGAAGCTGAA
    GGAGGAGATG CTGGTGATGA AGAGGGGGGT GGCGAGGAGG GACAACCAGA ATCAAGCTCT
    GCATTTGCCG ATTTCATTCA AAGCATCATG AATTTTCTGG GTATGTTCAC CTTCAGGAAT
    ATTCGCAGAA AGTACCGCAG ACTCCGCAAG ATGACCATTA AGGAGATCGT GGTAGGGCTT
    GCTACCTTCA TGTGGACGAT TCTCTTGGGC ATCCTCCATT TCATTTACAG TGTTTGTAAA
    GGCTTCTTCC TCCTCATCTG GCACACGCTG TTTGGAGGAG GTTTAGTTGA AGGGGCCAAA
    AACATCACAG TAACTGAGTT ACTAGCCAGC ATGCCAGATC CCACACAGGA CGAGGTGCAT
    GGGGACTTAC CTGGAGAACC GAGGGGAGGA GAGGAACAGG AGACTGGAGG GATAATGGAC
    TCGATGGATG CTGGAGGGGG AGAGGAAGAG GATGAGGATA TCCAGGAGAA AGATGGTGGA
    AAGATTCCTG GTATAGATAC ACCTGGTGGA CTTGGAGATA TGGGTGATAC AACTCCTGTA
    GAACCGCCGA CTCCCGAGGG TACCCCTTTG TTGAAGAGGA AAGCGCAACC AGATGAAGCA
    GGAGCTGAAC AGCCTCCTGC TATTGAAGAG CCTCCTCCTG AGCCTGAGAA AGCAGACATT
    GAGAATGGTG AGAAGGCTGA GAAGGAAGTG GAGGTCAAGC CTGAGCCAAT ACCAGAAGAA
    CCGAAACCTC AGAGAGCCAC CAAAGCCAAG AAGGAGAAGA AATCAGTCAA GGGTGCAGGA
    TTTGAGCTCT GGAATGAGCT GGATGTGCAA AGAAATAAAT TCATGAACTG CTTGTCTCGT
    AACTTTTACA ACATTCGTTT CCTGGCTCTG TTCCTTGCCT TTGCCTTGAA CTTCATTCTT
    CTCTTCTACA AGGTATCTGA TACTCCCCCC AGTTCTGATG AATTTGAAGG TTCTGGCATC
    TTTGAAGGCT CTGGGCTGTT TGAAGGCTCT GCGGAGGATT TTGAGGGCTC TGGTGGAGAG
    GATGGAGGTG ATGATGAGGA TGAAGAAGGA CCTGTGTATT ATTTTCTGGA AGAAAGCACG
    GGATACATGC AGCCCACTCT GTCCTTCCTG GCCATTTTGC ACACCGTCAT TGCTTTCATA
    TCCATCATTG GGTACAACTG CCTCAAGATT CCACTGGTCA TCTTTAAGAG AGAGAAAGAG
    TTGGCGAGAA AGCTGGAGTT TGATGGCCTT TACATCACTG AACAGCCGGC AGATGATGAC
    ATTAAAGGCC AGTGGGACAG ACTGGTGTTG AACACTCCCT CTTTTCCCAC CAATTACTGG
    GACAAATTTG TGAAGCGAAA GGTTCTGGAT AAATATGGAG ATATTTATGG AAGAGAGAGA
    ATCGCAGAGC TGCTGGGTAT GGATCTGGCA TCTCTGGATG TCAGCAAACA ACAGACAGAC
    AAGAAACCAG AAGAGCCAGA CAACTCTATG CTTGCATGGT TCACTTCCAT TGACATCAAG
    TATCAGATCT GGAAATTTGG AGTTGTGTTC ACAGACAATA CTTTCCTCTA CCTGGTGTGG
    TACACAGTGA TGTCCCTTCT TGGACATTAC AACAACTTTT TCTTTGCTTG TCACCTGCTG
    GACATCGCAA TGGGTGTTAA GACCCTGCGT ACAATTCTTT CCTCCGTCAC ACATAATGGA
    AAACAGCTGA TGATGACTGT GGGTTTGCTG GCTGTGGTTG TGTACCTCTA CACTGTGGTG
    GCCTTCAACT TCTTCAGGAA GTTCTACAAT AAGAGCGAGG ATGAGGATGA GCCGGATATG
    AAGTGTGACG ACATGATGAC GTGTTATTTG TTCCACATGT ATGTGGGTGT ACGGGCAGGT
    GGAGGTATAG GAGATGAGAT CGAGGACCCT GCTGGAGATG AGTATGAGCT CTATCGGGTG
    GTCTTCGACA TCACCTTCTT CTTCTTTGTC ATCGTCATCC TGCTGGCCAT CATTCAGGGT
    CTGATCATTG ATGCCTTTGG TGAACTGAGA GACCAGCAAG AGCAAGTGAA AGAGGACATG
    GAGACCAAAT GCTTTATATG TGGCATTGGG AGCGACTACT TTGATACAAC GCCCCATGGC
    TTTGAGACCC ATACGCTTGA AGAACACAAC CTGGCCAATT ACATGTTTTT CCTGATGTAC
    TTAATCAACA AGGATGAAAC AGAGCACACT GGCCAGGAAT CATACGTGTG GAAGATGTAC
    CAGGAGAGAT GCTGGGATTT CTTCCCTGCA GGAGACTGCT TCAGAAAGCA ATATGAAGAC
    CAGCTTGGAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009303777.1
    CDS1..15192
    Translation

    Target ORF information:

    RefSeq Version XM_009305502.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio ryanodine receptor 1a (skeletal) (ryr1a), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009305502.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    13261
    13321
    13381
    13441
    13501
    13561
    13621
    13681
    13741
    13801
    13861
    13921
    13981
    14041
    14101
    14161
    14221
    14281
    14341
    14401
    14461
    14521
    14581
    14641
    14701
    14761
    14821
    14881
    14941
    15001
    15061
    15121
    15181
    ATGGCAGAAG GAGCTGAGGG AGAAGAGGAG ATCCAATTCC TAAGAACAGA TGACCAGGTG 
    GTGCTACAGT GTACAGCTAC TATCCTGAAG GAGCAGATTA AACTCTGTCT GTCCTGTGAG
    GGTTTTGGGA ATCGCCTGTG TTTTTTAGAA ACCACATCTA ATGCTCAGAA TGTTCCTCCT
    GATCTGGCAA TATGCTGCTT TATCCTGGAG CAGTCTTTAT CTGTGCGGGC CCTGCAGGAG
    ATGCTGGCTA ACACTACTGA GCTTAATGAG GCAGTCGATT TGGATAAATG GTCTTCACAA
    GGAGGAGGCC ACCGCACGTT GTTGTATGGC CACGCTATCC TACTCAGACA TACTCACTCT
    GGCATGTACC TTAGTTGCTT GACCACATCA AGATCCCTCA CAGACAAACT GGCCTTTGAT
    GTGGGCTTGC AGGAGGACTC CACAGGTGAG GCCTGTTGGT GGACCATTCA CCCTGCATCC
    AAGCAGAGGT CAGAGGGTGA GAAGGTCAGA GTGGGCGATG ACATTATTCT GGTCAGCGTG
    TCCTCTGAGA GATATCTGCA CCTCTCCTAT GCCAGCGGAG ACCTGATGGT GGACGCTTCT
    TTCATGCAGA CCCTTTGGAA CATGAACCCA GTCTGTTCAG GCTGTGAGCT GGCAGAGGGG
    TTTCTGACTG GAGGGCATGT TCTTCGTCTA TTTCACGGGC ACATGGATGA GTGTTTAACA
    ATTCCTGGCG CGGAGCAGGG CGACGATCAG AGACGAAATG CTCACTATGA AGGTGGAGCC
    GTGTGCAGCC ATGCTAGATC TCTCTGGAGA CTGGAGCCTC TTCGCATTGG ATGGAGTGGT
    AGCCACATGA AGTGGGGTCA GTCATTCAGG GTGCGCCACA TCACAACAGG CAGGTATCTG
    TGTCTGGACG AAGAGAAAGG ACTCCTGATT GTGGACCCAG AAAAGGCAAA TACGAAGCAG
    TCAGCTTTCT GCTTTCGTGC ATCAAAGGAG AAGACAGAAG TAGCTCAGAA ACGTGATGTG
    GAGGGAATGG GGATCCCAGA GATCAAGTAT GGCGAGTCCA TGTGCTTTGT GCAGCACGTC
    GCTACAAGTC TGTGGCTCAC ATATGCCTCG GTGGATGCCA AGTCTGCTCG GCTGGGACCA
    CTGAAGAGGA AGGCTATCCT GCATCAGGAG GGTCACATGG ATGATGCTTT GACTGTGGCT
    CGCTCTCAGA CAGCTGAATC GCAGGCTGCC AGGATGATCT ACAACACGGC AGGACTTTTC
    ACTCAGTTCA TTAAGGGTTT GGACTCTCTA AGTGGGAAGA GTAAATCTCC AGGACCTTCC
    CCTTCTCTGC CTCTAGACGC CATAGTTCTG TCATTGCAGG ATCTCATCTT CTATTTCCGA
    CCTCCAGAAG AGGAGCTGGA ACATGAGGAG AAGCAGACCA AACTGCGCTC CTTAAGAAAC
    CGCCAGAATC TCTTCCAAGA GGAGGGCATG ATCACTCTTG TATTGGACTG TGTTGATCGG
    TTGAATGTCT ACAACACTTC AGCCCACTTC TCAGAATTTG CTGGAGAGGA CGCAGCCGAG
    TCGTGGAAAG AGATCGTCAA CTTATTTTAT GAGCTGCTGG CATCTCTGAT CAGAGGAAAC
    AGGGCAAACT GTGCTTTGTT CTGTGATAAT CTTGACTGGT TGGTCAGCAA ACTGGATCGT
    CTGGAGGCAT CCTCGGGTAT TCTGGAGGTG CTTTATTGTA TCCTAATTGA AAGCCCTGAG
    GTGCTTAACA TCATCCAGGA AAACCACATC AAATCTATCA TTGCACTTTT GGACAAACAT
    GGACGCAATC ACAAGGTTTT AGATGTTCTC TGCTCATTAT GCGTGTGTAA TGGTGTTGCT
    GTGAGGTCCA ATCAGAATCT GATCACTGAA AACCTGCTTC CCGGACGGGA TCTTCTCCTT
    CAGACCAACA TCATTAATTA TGTTACCAGT ATGAGACCCA ACATTTTTCT GGGCACGTGT
    GAAGGCTCGA CTCAGTATAA GAAGTGGTAC TATGAGTTGA TCGTTGACCA TGTGGAGTCT
    TTCCTTACCG CTCATCCATT TCACCTTCGT GTTGGCTGGG CTTTGACGGA GGGCTACAGC
    CCGTATCCAG GAGGTGGAGA AGGATGGGGA GGCAATGGAG TTGGAGATGA TCTACACTCG
    TATGGTTTCG ATGGGTTGAA TCTGTGGACA GGTCGTGTAC CACGTCAGGT GACGTCACCC
    AGTCAACACA TTCTCTCGGC TGAGGATGTG GTGAGCTGCT GTTTAGATCT AAGCGTTCCC
    AGCATCTCCT TTCGTATTAA TGGACATCCC GTTCAAGGCA TGTTCGAGAA CTTCAATCTG
    GATGGTCTTT TCTTCCCCGT GGTCAGCTTC TCAGCGGGGA TAAAAGTTCG TTTTCTCCTT
    GGCGGACGAC AAGGAGACTT CAAATTCCTC CCTCCTCCAG GTTATGCCCC GTGCTATGAG
    GCCGTTCTGC CCAAAGACAG ACTGCGGATT GAACCCATTA AAGAGTACAA GCACGACAAC
    GACGGTGTCC GAAACCTGCT GGGACCGACA CAGTCTCTTT CCCACACCGC CTTCACTCCC
    TGTCCGGTGG ACACTGTGCA GATTGTGCTT CCTCCACATC TAGAGCGCAT CAGAGAGAAA
    CTGGCCGAAA ATAGCCATGA GCTGTGGGCC GTCACTCGAA TTGAACAGGG TTGGACATAT
    GGACTATTTC GTGATGACAA CAAGAAGCTG CATCCCTGTC TAGTGGACTT CCAGAGCCTC
    CCGGATCCTG AGAAGAGCTA CAACCTGGCC ATGTCTGGAG AGACGCTGAA GACTCTTCTG
    GCTTTAGGGT GTCATGTAGG CATGGGTGAT GAAAAAGCAG AGGAGAACCT GAAGAAGATC
    AAACTTCCTA AAACGTATAT GCAGTGTAAC GGATACAAAC CTGCTCCACT GGATCTTAAC
    CATGTGAAGC TCACACCGAA TCAGAACACA CTTGTGGAAA GGCTGGCTGA AAATGGACAC
    AACGTCTGGG CTCGAGACCG TGTCCGCCAA GGGTGGACTT ACAGCATCAT TCAGGATATT
    GTGAACAAGC GCAACCCTCG TCTCGTGCCT TACATTCTGC TTGATGAAAA GACAAAAAAG
    ACCAATCGTG ACACTGTGTG TGCCGCTGTA CGGACTCTGA TTGGCTATGG CTACAATATT
    GAACCCCCTG ACCAGGAGAG CAGTGGTCAT GGGGGTGGCA ATGGCCGGAG TGATAAGATC
    CGTGTGTTCA GGGCAGAGAA ATCCTACGCA GTGACGCAGG GTAAATGGTA CTTTGAGTTC
    GAGGCAGTAA CAGTGGGAGA GATGAGAGTT GGGTGGGCAA GACCAAGCGT CCATGCAGAC
    AGAGAGCTGG GATCAGATGA CCTGGCCTAC GTGTTCAATG GCTTTAAGTC TCAGCGCTGG
    CACATTGGAA ATGAGCCGTT TGGCCGACAG TGGCAGTCTG GTGATGTCGT TGGCTGCATG
    ATAGACCTGA CGGAGCAGAA CATCATGTTT ACTTTGAATG GAGAGATGCT CATCAGTGAC
    TCTGGATCTG AGATGGCATT TAAAGACATT GAGATCGGAG AAGGCTTCAT CCCTGTATGC
    AGTCTGGGTA TGTCCCAGGT GGGTCGGATC AACCTTGGTC AAAATGTGAG CAGCCTGCGG
    TACTTTACAA TCTGTGGACT GCAGGAAGGT TTCGAGCCAT TTGCTATCAA CATGAAGAGA
    GATATTACAA TGTGGTTCAG CAAGAGCTTG CCCCAGTTTG TGCCCGTTCC AACAGATCAT
    CCTCATATTG AGGTGTCCCG TGTGGATGGA ACGGTGGAGA GCGCTCCCTG TCTGAAACTG
    ACTCACAGGA CATTCGGTTC TCAGAATGCC AACACAGACC TGTTGTTCTT CAGACTCAGT
    ATGCCGATTG AATTCCACGA AACCTTCAAG GTCCCGGTGG GAGCTTCACC CCTCACACGA
    ACCCTCACTA TCCCTGAGGA TGAGACTCTT GAGGTGGATC CCGAATCTGA ATTCGAGCTC
    CTGAAGAAGT CAGCCACTCG TAAGGAGCAA GATGAGAAAG AAAAAGAGCC GTCCGTCCCG
    AAAGAGCTGC CTGGAAGCAA AGAAAGCGAG AATGAGAAGG ACACCACCAC AGAGAAGAGC
    AAAAAGAGAG GATTTTTTTC CAAGGCGAAG AAGGCAGCGT TTATTGCCCC ACCACCAGTG
    GTTCCAACAA TGCCTCGTCT AATGGAGGAT GTTGTCCCAG ATGATCGAGA CGATGTTGAA
    ATTATCTCAA ACACAACAAC TTATTATTAT TCAGTCCGGG TGTTCGCCGG ACAGGAGCCC
    AGTGGAGTAT GGGTAGGCTG GGTGACTCCT GATTACCACT TGTACGACCT TCACTTTGAT
    CTTTGTAAAG TCAGAAACGT AACTGTTACA GTTGGAGACG ATAAAGGCAA TATTCATGAC
    AGTGTGAAAC GCAGTAATTG CTACATGGTG TGGGGTGGAG AGTTTAGCAG CTCACAGCAA
    ACCCGCATCA GTCAGGAGGA CTTTGTGATC GGTTGTTTGG TGGACCTGGA CACAGGACTG
    ATGACCTTTA CAGCCAATGG GAAAGAGATC AATACGTTCT ATCAGGTTGA ACCTAACACT
    AAGCTTTTCC CTGCTGTGTT TTGTTTGCCA TCAAGTCAGA ACATGTTACA AGTGGAACTC
    GGCAAACTGA AGAACATTAT GCCCATCTCA GCAGCCATGT TTCGCAGCGA CTGTAAGAAC
    CCGGTCCCTC AATGCCCTCC CAGGTTAGAT CTTCAGATGC TGACCCCTGT CATATGGAGC
    CGGATGCCTA ATCACTTTTT GTCGCCTGAA ACTGGCCGCG TGAGTGAGAG GCAGGGCTGG
    AAAGTGCAAT GCATGGAACC TCTCGTTATG ATGGCACTGC ACATCCCTGA AGAGAACAGG
    TGTATTGACA TCCTGGAACT GTCAGAACGT ACCGACCTGA TGAAGTTCCA TTACCACACA
    CTGAAACTGT ATGGCTCGGT TTGCGCTCTG GGGAACAATC GTGTCGCACA CGCCCTCTGC
    AGCCACGTGG ACGAATCGCA GCTCTTTTAC GCCATCGAAA ACACGTACCT GCCTGGCCCG
    ATCCGCAGTG GCTACTACGA CCTGCTCATC TCCATGCACC TAGAGTCTGC CAAACGCAAC
    CGACTCATGA CCAATAAGGA GTTCATTGTG CCCATGACGG ACGAGACTCG AAGCATCACC
    CTTTATTCGG ATGCTGAGAA GGCACATGCG TTACCTGGTG TCGGACTTAC CACATGTCTA
    CGCCCAAAAC TTCACTTTGC TCCAACTGGA TTTGTGGGCA CTAATGCTGA CCTTTACACC
    CTGAGCCCCA TGTTACCTCT TCAGATGCTT AAAGATCATG CCCTCAGCAT GCTTACAGAA
    GCTGTGCAGG ACGGTGGCCA AGCCATTCGA GATCCTGTAG GGGGCAGCGT TGAGTACCAC
    TTTGTGCCCA TCCTGAAGCT CATCAGCACC CTTCTCATTA TGGGTGTATT TGATGACGAC
    GATGTCAAGC ACATCCTGAA ACTGATCGAA CCATCCGTCT TCAGCAGCAA CAGTGAGACT
    CCAGCTGAAG AAACTGGAGA TCCTGAACAG CAGCTGGTCT GCAAAGAGGA AGGAGCGGAG
    GTAAAAGAGC AAGAGGAACC AGCAGACGGT GGAAATGATG GAGAGCTGCT GGATGAAGGG
    ATGGGAGAGG AAGAAGAGCT TGAGGAAGAG GAGGAGGAGG AAGATGAAGA GTATGAGGAG
    GAGGTGAAAA AAGAGGAGGA TGAACATGCT GCTGAAAAAA CAGACAGAGA GAAAACTACA
    GATGGAGAAA CTGAGAAGGA AACTGGTGCA GCAGAGGCTG AAGTAAGGGA GGAGGAGGAT
    GTTGGAGAAG GACTGCTGCA CATGAAGCTT CCAGAGTCTG TCAAACTCCA GATGTGTACT
    CTCCTGCAAT ATTTCTGTGA CTGTGAGCTG CGTCACAGAG TGGAAGCCAT CATCGCTTTC
    TCAGACAAGT TTGTTAACCA AGTGCAGACC AATCAGAGAC AGCGATATAA TGAGCTCATG
    CAAGCTTTTA CAATGAGTGC AGCAGAAACG GCCCGTAAGA CCCGCGAATT CCGCTCCCCT
    CCACAGGAAC AGGTGAACAT GTTGATGAAC TTTAAGAACT GTCCAGAGGA AGAAGATTGC
    CCCGTTCCAG AGGATGTCCG GAATGGCCTC CTGACATTTC ACCATGACCT TTTAGCTCAC
    TGTGGTGTGC ACATTGAAGA GGAGGAACAG GAAGAAGAGG TGGACACTTC TCTCAGTGGG
    CGCCTACGCA GACTGGTGGA AAAAGTTAAA AGCTTGCGAA AAAAGCCAGA AGCTGAGCCT
    GAGCCTGAAG AAGATAAAAA ACCAAGTACT CTGCAGGAGC TGATCTCCCA CACCATGATC
    TACTGGGCTC AAGAGTCTTT CATCCAGAAC CCTGAGCTGG TGCGCCTCAT GTTTAGTCTG
    CTACACCGGC AGTATGATGC TTTGGGTGAG CTGATTCGAG CTTTGCCCAA AGCCTATATC
    ATTAATGCTG TTTCTATACA AGACACAATG GAGCTCCTGG AGTGTCTGGG ACAGATACGC
    TCCTTGCTCA TCGTTCAGAT GGGACCAGAG GAAGAGAGGC TGATGATCCA GAGTATCGGA
    AATATTATGA GTAACAGGGT ATTTTACCAG CACCCCAACT TGATGAGAGC TCTGGGAATG
    CATGAGACAG TCATGGAGGT TATGGTGAAC GTACTCGGAG GCGGAGATTC CAAGGAGATC
    AGATTCCCTC GAATGGTTAC CAACTGCTGT CGTTTCCTGT GTTATTTCTG TCGTATCAGC
    AGACAGAATC AGCGCTCGAT GTTTGATCAT CTGGGCTACC TCCTGCAGAA CAGTGGCATT
    GGGCTGGGAA TGCGTGGCTC CACTCCTCTT GATGTTGCTG CTGCTTCCTG TATCGATAAT
    AATGAGCTTG CCCTGGCTCT CCAAGAGCAA GACCTGGAGA TGGTAGTAAA GTACCTGGCA
    GGCTGTGGTC TGCAGAGCTG CCCTATGCTT TTGTCAAAGG GTTACCCGGA TATTGGATGG
    AACCCAGTTG GAGGAGAAAA GTATCTGGAT TTCCTGCGCT TTGCTGTGTT TGTGAATGGA
    GAGAGTGTGG AGGAGAACGC TAATGTGGTG GTCCGGCTGC TGATCAGGAG GCCTGAGTGC
    TTTGGTCCTG CACTGAGAGG AGAAGGGGGC AATGGTCTGC TGGCTGCCAT TGAAGAAGCC
    ATCAAGATCT CAGAGGACCC TGCAAGAGAT GGACCCACTG TTAAAAAAGA CAGACGCTTC
    CCGATGTTTG GAGGTGAAGA GCAGCATGAG GAGAACAGAG TGCACCTAGG AAATGCCATC
    ATGTCATTTT ACTCAGCTCT TATTGATCTG CTGGGTCGCT GTGCTCCTGA GATGCATTTG
    ATCCAGGCTG GTAAAGGTGA AGCTTTGAGA ATCAGGGCTA TTCTTCGGTC GCTTGTGCCC
    ATTGAGGATC TGGTCGGTGT GATCAGCTTG TCAGTCCAGA TTCCTGCTTT TGGAAAAGAT
    AACAGCATTA TTGAGCCGAA GATGTCTGCT AGTTTTGTTC CTGATCATAA AGCACCAATG
    GTCCTGTTCC TGGACAGAGT TTATGGCATC GACAACCAGG ACTTCCTATT GCATGTACTA
    GAAGTGGGTT TCCTGCCTGA TATGAGGGCG GCAGCCTCCT TAGACACAGC TGCATTCAGC
    ACTACAGAAA TGGCCTTGGC TCTGAACCGT TACCTGTGCT CCGCCGTGCT TCCTCTCATC
    ACTAAATGTG CTCCTTTGTT CGCCGGGACG GACCACCGAG CCATCATGAT CGACTCCATG
    CTTCACACCA TCTACAGGCT GTCTCGAGGG CGCTCGCTAA CGAAAGCACA AAGAGATGTT
    ATTGAGGAAT GCCTCATGTC TTTGTGCAGG TATCTGCGCC CTTCCATGCT CCAGCACCTT
    CTCAGGCGGC TAGTATTTGA TGTGCCTATT CTGAATGAGT ATGCCAAAAT GCCCCTTAAG
    CTGCTTACCA ATCATTATGA GCGTTGTTGG AAATATTATT GCTTGCCTAA TGGCTGGGCT
    AACTTTGGCG TATCATCAGA AGAGGAGCTT CATCTGACCC GTAAACTCTT TTGGGGGATC
    TTTGAATCTC TGGCTCACAA GAAATTCGAT GCAGAGCTGT TCAAAATCGC CATGCCTTGT
    ATATGTGCGA TCGCTGGTGC AATTCCTCCT GATTATGTGG ACGCCAGCTA CTCCTCCAAA
    ACCGAAAAAA AGGCTTCTGT TGATGCTGAA GGGAACTTTG ACCCCAAACC TGTTGATACC
    ACTAACACTA TAATTCCCGA GAAGTTGGAC GGGTTCATCA ACAGATATGC AGAATATACG
    CACGACAGAT GGGCTTTTGA AAAAATTCAA AACAACTGGA CATTTGGTGA GGTGATGGAT
    GAAAATGCCA AGACTCATCC CATGCTCAGA CCTTACAAAA CATTCTCTGA AAAGGATAAA
    GAAATCTACC GCTGGCCTAT TAAAGAGTCC ATCAAGGCCA TGATTGCCTG GGAGTGGACA
    CTGGACAAAG CCAGAGATGG AGAGGATGAG AAAGTTGAGA AGAAAACAGC CCGTAAGATC
    TCACAGACTG CACAGGCAAC CTATGACCCC AGTCATGGCT ATAGCCCCCA ACCTATTGAT
    ATCTCAGGCA TGACACTGTC CAGAGAGCTA CAGTCCATGG CAGAGCAGCT CGCAGAAAAC
    TACCACAACA CCTGGGGCCG TAAGAAGAAA ATGGAATTAC AAGCAAAAGG AGGAGGTACA
    CACCCTTTGC TTGTGCCTTA TGACACACTG ACGGCTAAAG AAAAGGCACG AGATAGAGAG
    AAAGCGTATG AGCTACTCAA GTTTCTGCAG CTCAATGGAT ATGCTGTGAC AAGAGGGCTC
    AAAGACATGG AAAGTGACAT TTCATCTATC GAGAAGCGTT TTGCGTATGG CTTCCTACAG
    AAGCTTCTGA AGTGGATGGA CATTGCTCAG GAGTTCATAG CACATCTTGA GGCGGTTGTG
    AGCAGCGGTC GTGTGGAAAA GTCACCACAT GAGCAAGAAA TCAAGTTCTT TGCCAAGATT
    CTTTTGCCTT TGATAAATCA GTATTTTAAA AACCACTGCC TTTACTTCCT CTCCACACCG
    GCCAAAATCC TAGGCAGCGG CGGACACGCC TCAAACAAGG AAAAGGAGAT GATTGCAAGC
    ATTTTCTGTA AGATGTCTGC CCTGGTGCGA CACAGAGTGT TTCTCTTTGG CACGGATGCG
    CCTGCTATTG TCAACTGCCT TCATATCCTC GCACGTTCGC TTGATGCCAG GACGGTGATG
    AAATCCGGGC CTGAGATTGT GAAAGCAGGA CTGCGTTTGT TCTTTGAGAG TGCAGCTGAT
    GACATTGAAA AGATGGTGGA GAATCTCAAA CTTGGCAAAG TGTCCAAAGG CAATCAGCCA
    GTGAAAGGTG TGTCCCAGAA TATAAACTAC ACCACAACGG CTCTGCTTCC TGTCCTCACA
    TCACTGTTTG ACCACATCGC TCAACACCAA TTCGGAGATG ATGTTATCCT GGATGATTTG
    CAGATGTCCT GCTACCGAAT CATGTGTAGC ATCTACTCTC TTGGGACTGT AAAAACTCCA
    CATGCAGAGA GGCATAGACC AGCCCTTGGA GAATGTCTCG CTCATCTCGC TGCAGCAATG
    CCTGTTGCCT ACCTGGAGCC GCATCTTAAT GAATACAACG CCTTTTCAGT CTACACTACC
    AAGACACCGA GAGAAAGAGC TATCCTTGGC CTTCCAAACC AAGTTCAAGA GTTGTGTTTG
    GACATTCCTG AGCTTGACGT TTTACTGAAG GAGATCGGAG ACCTGGCTGA ATCAGGAGCT
    CGATACACTG AAATGCCACA TGTGATTGAG GTCACTCTGC CCATGCTGTG TAACTATCTG
    CCCCGCTGGT GGGAGAGAGG ACTGGAGAAC TTCCCGGATC TAGAGGGTAA GCTGTGCACC
    GACGTCACCT CCGAATCCCT CAACCAGCTT CTGGGAAGCA TCATGAAAAT CGTGGTCAAC
    AACTTGGGAA TCGACGAAGC CTCCTGGATG AAGAGACTTG CAGTGTTCTC CCAACCCATT
    GTGAGCCGAG CCAAACCAGA GATGCTCAAA TCCCACTTCA TCCCCACCAT GGAGAAGCTG
    AAGAAGCGAA CCTCTAAAGT TGTAGCCGAG GAGGAGCATC TGCGTTTGGA GGGAAAGTCT
    GAGGGAGATG AGGAAGAGGG AACTATCAGA GACGAGTTTG CAGTTCTCTG TAGAGACCTC
    TATGCTCTAT ATCCTCTTCT CATCCGCTAT GTGGATAACA ACAGAGCAAG ATGGCTGACG
    TTTCGAGACC CAGATGCTGA GGAGTTGTTC CGCATGGTGG GAGAGGTCTT CATCTTCTGG
    TCAAAATCTC ACAATTTCAA GCGAGAGGAG CAAAACTTTG TGGTGATGAA CGAAATCAAC
    AATATGTCCT TTCTCACTGC TGACAGTAAG AGCAAGATGA GCAAGGCCGG GGACTCTGAG
    GCTGGAGGCT CGGATGTGGA GCGTACCAAG AAAAAGAGAC GGGGGGATCG ATACTCTGTG
    CAAACGTCCC TCATTGTGGC CGCACTGAAG AAAATGCTTC CCATTGGACT TAACATGTGT
    TCTCCTGCAG ACCAGGAGCT GATTAATCTG GCAAAGACAC GTTATTCTCT GAAAGACACA
    GATGAGGAGG TCAGAGAGTT CTTGCAAAAT AACCTGCACC TTCAGGGAAA GGTTGATAAC
    CCATCTATGA GGTGGCAGAT GGCACTGTAT AAGGAAATGG CAGGAAAGGC TGAAGATGCA
    GATGCCCCAG AGAATGTGGT CAAGAGGGTT CAGGAGGTCT CTGCGGTCCT TTACCACATT
    GAAGTGACGG AGCACCCTTT CAAGTCCAAA AAGATGGTTT GGCACAAACT GCTGTCGAAG
    CAGCGGCGTA GAGCGGTGGT GGCCTGTTTC AGGATGACTC CACTTTACAA CTTGCCCAGA
    CACAGAGCAT CTAATATGTT CCTGGATGGG TACAAGCGCA ACTGGATAAG AAATGAAGGC
    TATTCCTTTG AGGACAGAAT GATTGATGAC CTGTCTAAAG CATTGGAGGA GGAAGAAGAG
    GAGGAAGAGG AAAAGGAGAC AAAGCCAGAC CCTCTTCATC AGCTCATCCT TCACTTTAGC
    CGCACAGCAC TCACTGAGAA AAGTAAACTG GATATAGATC ACTTATACAT GGCATATGCT
    GATATCATGG CTAAGAGTTG CCACATGGAT GAAGAGGATG AGGGTGGAGA GGAGGAGAAT
    GAGGGTGGAG AGGAGGAGAT GTCCTTTGAG GAAAAGGAAA TGGAAAAGCA AAGACTGCTC
    TACCAGCAGT CGCGTCTACA CAACCGAGGG GCTGCTGAAA TGGTCCTGCA GATGATCAGT
    GCCTGCAAGG GTGAGACTGG TTGTATGGTC TCTTCCACCC TAAAACTTGG CATTTCTATT
    TTAAATGGCG GGAACTGTGA AGTTCAGCAG AAAATGCTGG ACTACCTGAA AGACAAGAAA
    GATGTGGGTT TCTTTCTCAG TGTTCAGGCT TTAATGCAGA CCTGCAGTGT TTTGGACCTG
    AATGCCTTTG AGAGGCAGAA TAAAGCTGAG GGTCTAGGTA TGGTGTCAGA AGAGGGTACA
    AGTGAGAAGG TCATGGCTGA TGACGAGTTC ACTTGCGACC TCTTCCGCTT TCTCCAGCTC
    CTCTGTGAGG GTCACAACAA TGACTTCCAA AATTACCTCA GAACACAGAC AGGCAGTACC
    ACCACCATCA ATGTCATTAT CTGTACAGTG GATTACCTGC TTAGACTACA GGAGTCCATC
    AGTGATTTCT ACTGGTATTA CTCTGGAAAA GACATCATAG ATGAACCAGG AAAGCGAAAC
    TTCTCTAAAG CTATGACTGT GGCCAAACAG GTTTTCAACA GTCTTACTGA GTATATCCAG
    GGTCCCTGCA CAGGAAACCA GCAGTCCCTG GCTCACAGTC GCTTATGGGA TGCTGTGGTG
    GGCTTTCTGC ACGTGTTTGC TCATATGATG ATGAAACTGG CTCAGGGAAG AGATTCCAGT
    CAGATTGGTC TTCTGAAAGA GTTGTTGGAC CTTCAGAAAG ACATGGTGGT CATGTTATTG
    TCCTTGTTGG AGGGTAATGT TGTAAATGGC ACCATTGCTC GCCAGATGGT GGACATGCTA
    GTGGAATCCT CCAGCAATGT AGAAATGATC CTTAAGTTCT TCGACATGTT CCTCAAGCTG
    AAGGACATTG TTGCGTCTGA CGCGTTTCGT GACTACGTGA CCGACCCTAG AGGTTTGATA
    TCCAAGAAAG ACTTTCAGAA ATCCATGGAC AGCCAGAAAC AGTACACACC TTCAGAGATC
    CAATTCCTAT TGTCATGCTC TGAGGCTGAT GAGAATGAGA TGATCAATTA CGAGGAGTTT
    GCAAGTCGCT TTCAAGAGCC GGCCAAAGAC ATTGGTTTTA ACATAGCGGT GCTGCTAACC
    AACCTCTCCG AACATGTTCC TCACGATACA AGACTGCAGA ACTTCCTCGA ACAAGCCGAG
    AGCGTCCTGA ACTATTTCCG CCCATTCTTG GGTCGCATCG AGATCATGGG CGCCAGCAGG
    AAAATCGAGA GAATCTACTT CGAGATCAGC GAGGTGAATC GCAAGCAGTG GGAGATGCCT
    CAAGTCAAAG AATCAAAGAG ACAGTTCATA TTCGATGTAG TCAATGAAGG CGGCGAATCG
    GAGAAGATGG AGCTTTTCGT CAACTTCTGC GAGGACACCA TATTCGAGAT GAACATTGCG
    TCGCAGATTT CAGAACAGGA CGAGGAGGAG AAAGAAGAGG ATGATATTGA GGAAGCTGAA
    GGAGGAGATG CTGGTGATGA AGAGGGGGGT GGCGAGGAGG GACAACCAGA ATCAAGCTCT
    GCATTTGCCG ATTTCATTCA AAGCATCATG AATTTTCTGG GTATGTTCAC CTTCAGGAAT
    ATTCGCAGAA AGTACCGCAG ACTCCGCAAG ATGACCATTA AGGAGATCGT GGTAGGGCTT
    GCTACCTTCA TGTGGACGAT TCTCTTGGGC ATCCTCCATT TCATTTACAG TGTTTGTAAA
    GGCTTCTTCC TCCTCATCTG GCACACGCTG TTTGGAGGAG GTTTAGTTGA AGGGGCCAAA
    AACATCACAG TAACTGAGTT ACTAGCCAGC ATGCCAGATC CCACACAGGA CGAGGTGCAT
    GGGGACTTAC CTGGAGAACC GAGGGGAGGA GAGGAACAGG AGACTGGAGG GATAATGGAC
    TCGATGGATG CTGGAGGGGG AGAGGAAGAG GATGAGGATA TCCAGGAGAA AGATGGTGGA
    AAGATTCCTG GTATAGATAC ACCTGGTGGA CTTGGAGATA TGGGTGATAC AACTCCTGTA
    GAACCGCCGA CTCCCGAGGG TACCCCTTTG TTGAAGAGGA AAGCGCAACC AGATGAAGCA
    GGAGCTGAAC AGCCTCCTGC TATTGAAGAG CCTCCTCCTG AGCCTGAGAA AGCAGACATT
    GAGAATGGTG AGAAGGCTGA GAAGGAAGTG GAGGTCAAGC CTGAGCCAAT ACCAGAAGAA
    CCGAAACCTC AGAGAGCCAC CAAAGCCAAG AAGGAGAAGA AATCAGTCAA GGGTGCAGGA
    TTTGAGCTCT GGAATGAGCT GGATGTGCAA AGAAATAAAT TCATGAACTG CTTGTCTCGT
    AACTTTTACA ACATTCGTTT CCTGGCTCTG TTCCTTGCCT TTGCCTTGAA CTTCATTCTT
    CTCTTCTACA AGGTATCTGA TACTCCCCCC AGTTCTGATG AATTTGAAGG TTCTGGCATC
    TTTGAAGGCT CTGGGCTGTT TGAAGGCTCT GCGGAGGATT TTGAGGGCTC TGGTGGAGAG
    GATGGAGGTG ATGATGAGGA TGAAGAAGGA CCTGTGTATT ATTTTCTGGA AGAAAGCACG
    GGATACATGC AGCCCACTCT GTCCTTCCTG GCCATTTTGC ACACCGTCAT TGCTTTCATA
    TCCATCATTG GGTACAACTG CCTCAAGATT CCACTGGTCA TCTTTAAGAG AGAGAAAGAG
    TTGGCGAGAA AGCTGGAGTT TGATGGCCTT TACATCACTG AACAGCCGGC AGATGATGAC
    ATTAAAGGCC AGTGGGACAG ACTGGTGTTG AACACTCCCT CTTTTCCCAC CAATTACTGG
    GACAAATTTG TGAAGCGAAA GGTTCTGGAT AAATATGGAG ATATTTATGG AAGAGAGAGA
    ATCGCAGAGC TGCTGGGTAT GGATCTGGCA TCTCTGGATG TCAGCAAACA ACAGACAGAC
    AAGAAACCAG AAGAGCCAGA CAACTCTATG CTTGCATGGT TCACTTCCAT TGACATCAAG
    TATCAGATCT GGAAATTTGG AGTTGTGTTC ACAGACAATA CTTTCCTCTA CCTGGTGTGG
    TACACAGTGA TGTCCCTTCT TGGACATTAC AACAACTTTT TCTTTGCTTG TCACCTGCTG
    GACATCGCAA TGGGTGTTAA GACCCTGCGT ACAATTCTTT CCTCCGTCAC ACATAATGGA
    AAACAGCTGA TGATGACTGT GGGTTTGCTG GCTGTGGTTG TGTACCTCTA CACTGTGGTG
    GCCTTCAACT TCTTCAGGAA GTTCTACAAT AAGAGCGAGG ATGAGGATGA GCCGGATATG
    AAGTGTGACG ACATGATGAC GTGTTATTTG TTCCACATGT ATGTGGGTGT ACGGGCAGGT
    GGAGGTATAG GAGATGAGAT CGAGGACCCT GCTGGAGATG AGTATGAGCT CTATCGGGTG
    GTCTTCGACA TCACCTTCTT CTTCTTTGTC ATCGTCATCC TGCTGGCCAT CATTCAGGGT
    CTGATCATTG ATGCCTTTGG TGAACTGAGA GACCAGCAAG AGCAAGTGAA AGAGGACATG
    GAGACCAAAT GCTTTATATG TGGCATTGGG AGCGACTACT TTGATACAAC GCCCCATGGC
    TTTGAGACCC ATACGCTTGA AGAACACAAC CTGGCCAATT ACATGTTTTT CCTGATGTAC
    TTAATCAACA AGGATGAAAC AGAGCACACT GGCCAGGAAT CATACGTGTG GAAGATGTAC
    CAGGAGAGAT GCTGGGATTT CTTCCCTGCA GGAGACTGCT TCAGAAAGCA ATATGAAGAC
    CAGCTTGGAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa47274
    Clone ID Related Accession (Same CDS sequence) XM_009305502.3 , XM_009305502.2
    Accession Version XM_009305502.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 15192bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product ryanodine receptor 1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009305502.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    ATGGCAGAAG GAGCTGAGGG AGAAGAGGAG ATCCAATTCC TAAGAACAGA TGACCAGGTG 
    GTGCTACAGT GTACAGCTAC TATCCTGAAG GAGCAGATTA AACTCTGTCT GTCCTGTGAG
    GGTTTTGGGA ATCGCCTGTG TTTTTTAGAA ACCACATCTA ATGCTCAGAA TGTTCCTCCT
    GATCTGGCAA TATGCTGCTT TATCCTGGAG CAGTCTTTAT CTGTGCGGGC CCTGCAGGAG
    ATGCTGGCTA ACACTACTGA GCTTAATGAG GCAGTCGATT TGGATAAATG GTCTTCACAA
    GGAGGAGGCC ACCGCACGTT GTTGTATGGC CACGCTATCC TACTCAGACA TACTCACTCT
    GGCATGTACC TTAGTTGCTT GACCACATCA AGATCCCTCA CAGACAAACT GGCCTTTGAT
    GTGGGCTTGC AGGAGGACTC CACAGGTGAG GCCTGTTGGT GGACCATTCA CCCTGCATCC
    AAGCAGAGGT CAGAGGGTGA GAAGGTCAGA GTGGGCGATG ACATTATTCT GGTCAGCGTG
    TCCTCTGAGA GATATCTGCA CCTCTCCTAT GCCAGCGGAG ACCTGATGGT GGACGCTTCT
    TTCATGCAGA CCCTTTGGAA CATGAACCCA GTCTGTTCAG GCTGTGAGCT GGCAGAGGGG
    TTTCTGACTG GAGGGCATGT TCTTCGTCTA TTTCACGGGC ACATGGATGA GTGTTTAACA
    ATTCCTGGCG CGGAGCAGGG CGACGATCAG AGACGAAATG CTCACTATGA AGGTGGAGCC
    GTGTGCAGCC ATGCTAGATC TCTCTGGAGA CTGGAGCCTC TTCGCATTGG ATGGAGTGGT
    AGCCACATGA AGTGGGGTCA GTCATTCAGG GTGCGCCACA TCACAACAGG CAGGTATCTG
    TGTCTGGACG AAGAGAAAGG ACTCCTGATT GTGGACCCAG AAAAGGCAAA TACGAAGCAG
    TCAGCTTTCT GCTTTCGTGC ATCAAAGGAG AAGACAGAAG TAGCTCAGAA ACGTGATGTG
    GAGGGAATGG GGATCCCAGA GATCAAGTAT GGCGAGTCCA TGTGCTTTGT GCAGCACGTC
    GCTACAAGTC TGTGGCTCAC ATATGCCTCG GTGGATGCCA AGTCTGCTCG GCTGGGACCA
    CTGAAGAGGA AGGCTATCCT GCATCAGGAG GGTCACATGG ATGATGCTTT GACTGTGGCT
    CGCTCTCAGA CAGCTGAATC GCAGGCTGCC AGGATGATCT ACAACACGGC AGGACTTTTC
    ACTCAGTTCA TTAAGGGTTT GGACTCTCTA AGTGGGAAGA GTAAATCTCC AGGACCTTCC
    CCTTCTCTGC CTCTAGACGC CATAGTTCTG TCATTGCAGG ATCTCATCTT CTATTTCCGA
    CCTCCAGAAG AGGAGCTGGA ACATGAGGAG AAGCAGACCA AACTGCGCTC CTTAAGAAAC
    CGCCAGAATC TCTTCCAAGA GGAGGGCATG ATCACTCTTG TATTGGACTG TGTTGATCGG
    TTGAATGTCT ACAACACTTC AGCCCACTTC TCAGAATTTG CTGGAGAGGA CGCAGCCGAG
    TCGTGGAAAG AGATCGTCAA CTTATTTTAT GAGCTGCTGG CATCTCTGAT CAGAGGAAAC
    AGGGCAAACT GTGCTTTGTT CTGTGATAAT CTTGACTGGT TGGTCAGCAA ACTGGATCGT
    CTGGAGGCAT CCTCGGGTAT TCTGGAGGTG CTTTATTGTA TCCTAATTGA AAGCCCTGAG
    GTGCTTAACA TCATCCAGGA AAACCACATC AAATCTATCA TTGCACTTTT GGACAAACAT
    GGACGCAATC ACAAGGTTTT AGATGTTCTC TGCTCATTAT GCGTGTGTAA TGGTGTTGCT
    GTGAGGTCCA ATCAGAATCT GATCACTGAA AACCTGCTTC CCGGACGGGA TCTTCTCCTT
    CAGACCAACA TCATTAATTA TGTTACCAGT ATGAGACCCA ACATTTTTCT GGGCACGTGT
    GAAGGCTCGA CTCAGTATAA GAAGTGGTAC TATGAGTTGA TCGTTGACCA TGTGGAGTCT
    TTCCTTACCG CTCATCCATT TCACCTTCGT GTTGGCTGGG CTTTGACGGA GGGCTACAGC
    CCGTATCCAG GAGGTGGAGA AGGATGGGGA GGCAATGGAG TTGGAGATGA TCTACACTCG
    TATGGTTTCG ATGGGTTGAA TCTGTGGACA GGTCGTGTAC CACGTCAGGT GACGTCACCC
    AGTCAACACA TTCTCTCGGC TGAGGATGTG GTGAGCTGCT GTTTAGATCT AAGCGTTCCC
    AGCATCTCCT TTCGTATTAA TGGACATCCC GTTCAAGGCA TGTTCGAGAA CTTCAATCTG
    GATGGTCTTT TCTTCCCCGT GGTCAGCTTC TCAGCGGGGA TAAAAGTTCG TTTTCTCCTT
    GGCGGACGAC AAGGAGACTT CAAATTCCTC CCTCCTCCAG GTTATGCCCC GTGCTATGAG
    GCCGTTCTGC CCAAAGACAG ACTGCGGATT GAACCCATTA AAGAGTACAA GCACGACAAC
    GACGGTGTCC GAAACCTGCT GGGACCGACA CAGTCTCTTT CCCACACCGC CTTCACTCCC
    TGTCCGGTGG ACACTGTGCA GATTGTGCTT CCTCCACATC TAGAGCGCAT CAGAGAGAAA
    CTGGCCGAAA ATAGCCATGA GCTGTGGGCC GTCACTCGAA TTGAACAGGG TTGGACATAT
    GGACTATTTC GTGATGACAA CAAGAAGCTG CATCCCTGTC TAGTGGACTT CCAGAGCCTC
    CCGGATCCTG AGAAGAGCTA CAACCTGGCC ATGTCTGGAG AGACGCTGAA GACTCTTCTG
    GCTTTAGGGT GTCATGTAGG CATGGGTGAT GAAAAAGCAG AGGAGAACCT GAAGAAGATC
    AAACTTCCTA AAACGTATAT GCAGTGTAAC GGATACAAAC CTGCTCCACT GGATCTTAAC
    CATGTGAAGC TCACACCGAA TCAGAACACA CTTGTGGAAA GGCTGGCTGA AAATGGACAC
    AACGTCTGGG CTCGAGACCG TGTCCGCCAA GGGTGGACTT ACAGCATCAT TCAGGATATT
    GTGAACAAGC GCAACCCTCG TCTCGTGCCT TACATTCTGC TTGATGAAAA GACAAAAAAG
    ACCAATCGTG ACACTGTGTG TGCCGCTGTA CGGACTCTGA TTGGCTATGG CTACAATATT
    GAACCCCCTG ACCAGGAGAG CAGTGGTCAT GGGGGTGGCA ATGGCCGGAG TGATAAGATC
    CGTGTGTTCA GGGCAGAGAA ATCCTACGCA GTGACGCAGG GTAAATGGTA CTTTGAGTTC
    GAGGCAGTAA CAGTGGGAGA GATGAGAGTT GGGTGGGCAA GACCAAGCGT CCATGCAGAC
    AGAGAGCTGG GATCAGATGA CCTGGCCTAC GTGTTCAATG GCTTTAAGTC TCAGCGCTGG
    CACATTGGAA ATGAGCCGTT TGGCCGACAG TGGCAGTCTG GTGATGTCGT TGGCTGCATG
    ATAGACCTGA CGGAGCAGAA CATCATGTTT ACTTTGAATG GAGAGATGCT CATCAGTGAC
    TCTGGATCTG AGATGGCATT TAAAGACATT GAGATCGGAG AAGGCTTCAT CCCTGTATGC
    AGTCTGGGTA TGTCCCAGGT GGGTCGGATC AACCTTGGTC AAAATGTGAG CAGCCTGCGG
    TACTTTACAA TCTGTGGACT GCAGGAAGGT TTCGAGCCAT TTGCTATCAA CATGAAGAGA
    GATATTACAA TGTGGTTCAG CAAGAGCTTG CCCCAGTTTG TGCCCGTTCC AACAGATCAT
    CCTCATATTG AGGTGTCCCG TGTGGATGGA ACGGTGGAGA GCGCTCCCTG TCTGAAACTG
    ACTCACAGGA CATTCGGTTC TCAGAATGCC AACACAGACC TGTTGTTCTT CAGACTCAGT
    ATGCCGATTG AATTCCACGA AACCTTCAAG GTCCCGGTGG GAGCTTCACC CCTCACACGA
    ACCCTCACTA TCCCTGAGGA TGAGACTCTT GAGGTGGATC CCGAATCTGA ATTCGAGCTC
    CTGAAGAAGT CAGCCACTCG TAAGGAGCAA GATGAGAAAG AAAAAGAGCC GTCCGTCCCG
    AAAGAGCTGC CTGGAAGCAA AGAAAGCGAG AATGAGAAGG ACACCACCAC AGAGAAGAGC
    AAAAAGAGAG GATTTTTTTC CAAGGCGAAG AAGGCAGCGT TTATTGCCCC ACCACCAGTG
    GTTCCAACAA TGCCTCGTCT AATGGAGGAT GTTGTCCCAG ATGATCGAGA CGATGTTGAA
    ATTATCTCAA ACACAACAAC TTATTATTAT TCAGTCCGGG TGTTCGCCGG ACAGGAGCCC
    AGTGGAGTAT GGGTAGGCTG GGTGACTCCT GATTACCACT TGTACGACCT TCACTTTGAT
    CTTTGTAAAG TCAGAAACGT AACTGTTACA GTTGGAGACG ATAAAGGCAA TATTCATGAC
    AGTGTGAAAC GCAGTAATTG CTACATGGTG TGGGGTGGAG AGTTTAGCAG CTCACAGCAA
    ACCCGCATCA GTCAGGAGGA CTTTGTGATC GGTTGTTTGG TGGACCTGGA CACAGGACTG
    ATGACCTTTA CAGCCAATGG GAAAGAGATC AATACGTTCT ATCAGGTTGA ACCTAACACT
    AAGCTTTTCC CTGCTGTGTT TTGTTTGCCA TCAAGTCAGA ACATGTTACA AGTGGAACTC
    GGCAAACTGA AGAACATTAT GCCCATCTCA GCAGCCATGT TTCGCAGCGA CTGTAAGAAC
    CCGGTCCCTC AATGCCCTCC CAGGTTAGAT CTTCAGATGC TGACCCCTGT CATATGGAGC
    CGGATGCCTA ATCACTTTTT GTCGCCTGAA ACTGGCCGCG TGAGTGAGAG GCAGGGCTGG
    AAAGTGCAAT GCATGGAACC TCTCGTTATG ATGGCACTGC ACATCCCTGA AGAGAACAGG
    TGTATTGACA TCCTGGAACT GTCAGAACGT ACCGACCTGA TGAAGTTCCA TTACCACACA
    CTGAAACTGT ATGGCTCGGT TTGCGCTCTG GGGAACAATC GTGTCGCACA CGCCCTCTGC
    AGCCACGTGG ACGAATCGCA GCTCTTTTAC GCCATCGAAA ACACGTACCT GCCTGGCCCG
    ATCCGCAGTG GCTACTACGA CCTGCTCATC TCCATGCACC TAGAGTCTGC CAAACGCAAC
    CGACTCATGA CCAATAAGGA GTTCATTGTG CCCATGACGG ACGAGACTCG AAGCATCACC
    CTTTATTCGG ATGCTGAGAA GGCACATGCG TTACCTGGTG TCGGACTTAC CACATGTCTA
    CGCCCAAAAC TTCACTTTGC TCCAACTGGA TTTGTGGGCA CTAATGCTGA CCTTTACACC
    CTGAGCCCCA TGTTACCTCT TCAGATGCTT AAAGATCATG CCCTCAGCAT GCTTACAGAA
    GCTGTGCAGG ACGGTGGCCA AGCCATTCGA GATCCTGTAG GGGGCAGCGT TGAGTACCAC
    TTTGTGCCCA TCCTGAAGCT CATCAGCACC CTTCTCATTA TGGGTGTATT TGATGACGAC
    GATGTCAAGC ACATCCTGAA ACTGATCGAA CCATCCGTCT TCAGCAGCAA CAGTGAGACT
    CCAGCTGAAG AAACTGGAGA TCCTGAACAG CAGCTGGTCT GCAAAGAGGA AGGAGCGGAG
    GTAAAAGAGC AAGAGGAACC AGCAGACGGT GGAAATGATG GAGAGCTGCT GGATGAAGGG
    ATGGGAGAGG AAGAAGAGCT TGAGGAAGAG GAGGAGGAGG AAGATGAAGA GTATGAGGAG
    GAGGTGAAAA AAGAGGAGGA TGAACATGCT GCTGAAAAAA CAGACAGAGA GAAAACTACA
    GATGGAGAAA CTGAGAAGGA AACTGGTGCA GCAGAGGCTG AAGTAAGGGA GGAGGAGGAT
    GTTGGAGAAG GACTGCTGCA CATGAAGCTT CCAGAGTCTG TCAAACTCCA GATGTGTACT
    CTCCTGCAAT ATTTCTGTGA CTGTGAGCTG CGTCACAGAG TGGAAGCCAT CATCGCTTTC
    TCAGACAAGT TTGTTAACCA AGTGCAGACC AATCAGAGAC AGCGATATAA TGAGCTCATG
    CAAGCTTTTA CAATGAGTGC AGCAGAAACG GCCCGTAAGA CCCGCGAATT CCGCTCCCCT
    CCACAGGAAC AGGTGAACAT GTTGATGAAC TTTAAGAACT GTCCAGAGGA AGAAGATTGC
    CCCGTTCCAG AGGATGTCCG GAATGGCCTC CTGACATTTC ACCATGACCT TTTAGCTCAC
    TGTGGTGTGC ACATTGAAGA GGAGGAACAG GAAGAAGAGG TGGACACTTC TCTCAGTGGG
    CGCCTACGCA GACTGGTGGA AAAAGTTAAA AGCTTGCGAA AAAAGCCAGA AGCTGAGCCT
    GAGCCTGAAG AAGATAAAAA ACCAAGTACT CTGCAGGAGC TGATCTCCCA CACCATGATC
    TACTGGGCTC AAGAGTCTTT CATCCAGAAC CCTGAGCTGG TGCGCCTCAT GTTTAGTCTG
    CTACACCGGC AGTATGATGC TTTGGGTGAG CTGATTCGAG CTTTGCCCAA AGCCTATATC
    ATTAATGCTG TTTCTATACA AGACACAATG GAGCTCCTGG AGTGTCTGGG ACAGATACGC
    TCCTTGCTCA TCGTTCAGAT GGGACCAGAG GAAGAGAGGC TGATGATCCA GAGTATCGGA
    AATATTATGA GTAACAGGGT ATTTTACCAG CACCCCAACT TGATGAGAGC TCTGGGAATG
    CATGAGACAG TCATGGAGGT TATGGTGAAC GTACTCGGAG GCGGAGATTC CAAGGAGATC
    AGATTCCCTC GAATGGTTAC CAACTGCTGT CGTTTCCTGT GTTATTTCTG TCGTATCAGC
    AGACAGAATC AGCGCTCGAT GTTTGATCAT CTGGGCTACC TCCTGCAGAA CAGTGGCATT
    GGGCTGGGAA TGCGTGGCTC CACTCCTCTT GATGTTGCTG CTGCTTCCTG TATCGATAAT
    AATGAGCTTG CCCTGGCTCT CCAAGAGCAA GACCTGGAGA TGGTAGTAAA GTACCTGGCA
    GGCTGTGGTC TGCAGAGCTG CCCTATGCTT TTGTCAAAGG GTTACCCGGA TATTGGATGG
    AACCCAGTTG GAGGAGAAAA GTATCTGGAT TTCCTGCGCT TTGCTGTGTT TGTGAATGGA
    GAGAGTGTGG AGGAGAACGC TAATGTGGTG GTCCGGCTGC TGATCAGGAG GCCTGAGTGC
    TTTGGTCCTG CACTGAGAGG AGAAGGGGGC AATGGTCTGC TGGCTGCCAT TGAAGAAGCC
    ATCAAGATCT CAGAGGACCC TGCAAGAGAT GGACCCACTG TTAAAAAAGA CAGACGCTTC
    CCGATGTTTG GAGGTGAAGA GCAGCATGAG GAGAACAGAG TGCACCTAGG AAATGCCATC
    ATGTCATTTT ACTCAGCTCT TATTGATCTG CTGGGTCGCT GTGCTCCTGA GATGCATTTG
    ATCCAGGCTG GTAAAGGTGA AGCTTTGAGA ATCAGGGCTA TTCTTCGGTC GCTTGTGCCC
    ATTGAGGATC TGGTCGGTGT GATCAGCTTG TCAGTCCAGA TTCCTGCTTT TGGAAAAGAT
    AACAGCATTA TTGAGCCGAA GATGTCTGCT AGTTTTGTTC CTGATCATAA AGCACCAATG
    GTCCTGTTCC TGGACAGAGT TTATGGCATC GACAACCAGG ACTTCCTATT GCATGTACTA
    GAAGTGGGTT TCCTGCCTGA TATGAGGGCG GCAGCCTCCT TAGACACAGC TGCATTCAGC
    ACTACAGAAA TGGCCTTGGC TCTGAACCGT TACCTGTGCT CCGCCGTGCT TCCTCTCATC
    ACTAAATGTG CTCCTTTGTT CGCCGGGACG GACCACCGAG CCATCATGAT CGACTCCATG
    CTTCACACCA TCTACAGGCT GTCTCGAGGG CGCTCGCTAA CGAAAGCACA AAGAGATGTT
    ATTGAGGAAT GCCTCATGTC TTTGTGCAGG TATCTGCGCC CTTCCATGCT CCAGCACCTT
    CTCAGGCGGC TAGTATTTGA TGTGCCTATT CTGAATGAGT ATGCCAAAAT GCCCCTTAAG
    CTGCTTACCA ATCATTATGA GCGTTGTTGG AAATATTATT GCTTGCCTAA TGGCTGGGCT
    AACTTTGGCG TATCATCAGA AGAGGAGCTT CATCTGACCC GTAAACTCTT TTGGGGGATC
    TTTGAATCTC TGGCTCACAA GAAATTCGAT GCAGAGCTGT TCAAAATCGC CATGCCTTGT
    ATATGTGCGA TCGCTGGTGC AATTCCTCCT GATTATGTGG ACGCCAGCTA CTCCTCCAAA
    ACCGAAAAAA AGGCTTCTGT TGATGCTGAA GGGAACTTTG ACCCCAAACC TGTTGATACC
    ACTAACACTA TAATTCCCGA GAAGTTGGAC GGGTTCATCA ACAGATATGC AGAATATACG
    CACGACAGAT GGGCTTTTGA AAAAATTCAA AACAACTGGA CATTTGGTGA GGTGATGGAT
    GAAAATGCCA AGACTCATCC CATGCTCAGA CCTTACAAAA CATTCTCTGA AAAGGATAAA
    GAAATCTACC GCTGGCCTAT TAAAGAGTCC ATCAAGGCCA TGATTGCCTG GGAGTGGACA
    CTGGACAAAG CCAGAGATGG AGAGGATGAG AAAGTTGAGA AGAAAACAGC CCGTAAGATC
    TCACAGACTG CACAGGCAAC CTATGACCCC AGTCATGGCT ATAGCCCCCA ACCTATTGAT
    ATCTCAGGCA TGACACTGTC CAGAGAGCTA CAGTCCATGG CAGAGCAGCT CGCAGAAAAC
    TACCACAACA CCTGGGGCCG TAAGAAGAAA ATGGAATTAC AAGCAAAAGG AGGAGGTACA
    CACCCTTTGC TTGTGCCTTA TGACACACTG ACGGCTAAAG AAAAGGCACG AGATAGAGAG
    AAAGCGTATG AGCTACTCAA GTTTCTGCAG CTCAATGGAT ATGCTGTGAC AAGAGGGCTC
    AAAGACATGG AAAGTGACAT TTCATCTATC GAGAAGCGTT TTGCGTATGG CTTCCTACAG
    AAGCTTCTGA AGTGGATGGA CATTGCTCAG GAGTTCATAG CACATCTTGA GGCGGTTGTG
    AGCAGCGGTC GTGTGGAAAA GTCACCACAT GAGCAAGAAA TCAAGTTCTT TGCCAAGATT
    CTTTTGCCTT TGATAAATCA GTATTTTAAA AACCACTGCC TTTACTTCCT CTCCACACCG
    GCCAAAATCC TAGGCAGCGG CGGACACGCC TCAAACAAGG AAAAGGAGAT GATTGCAAGC
    ATTTTCTGTA AGATGTCTGC CCTGGTGCGA CACAGAGTGT TTCTCTTTGG CACGGATGCG
    CCTGCTATTG TCAACTGCCT TCATATCCTC GCACGTTCGC TTGATGCCAG GACGGTGATG
    AAATCCGGGC CTGAGATTGT GAAAGCAGGA CTGCGTTTGT TCTTTGAGAG TGCAGCTGAT
    GACATTGAAA AGATGGTGGA GAATCTCAAA CTTGGCAAAG TGTCCAAAGG CAATCAGCCA
    GTGAAAGGTG TGTCCCAGAA TATAAACTAC ACCACAACGG CTCTGCTTCC TGTCCTCACA
    TCACTGTTTG ACCACATCGC TCAACACCAA TTCGGAGATG ATGTTATCCT GGATGATTTG
    CAGATGTCCT GCTACCGAAT CATGTGTAGC ATCTACTCTC TTGGGACTGT AAAAACTCCA
    CATGCAGAGA GGCATAGACC AGCCCTTGGA GAATGTCTCG CTCATCTCGC TGCAGCAATG
    CCTGTTGCCT ACCTGGAGCC GCATCTTAAT GAATACAACG CCTTTTCAGT CTACACTACC
    AAGACACCGA GAGAAAGAGC TATCCTTGGC CTTCCAAACC AAGTTCAAGA GTTGTGTTTG
    GACATTCCTG AGCTTGACGT TTTACTGAAG GAGATCGGAG ACCTGGCTGA ATCAGGAGCT
    CGATACACTG AAATGCCACA TGTGATTGAG GTCACTCTGC CCATGCTGTG TAACTATCTG
    CCCCGCTGGT GGGAGAGAGG ACTGGAGAAC TTCCCGGATC TAGAGGGTAA GCTGTGCACC
    GACGTCACCT CCGAATCCCT CAACCAGCTT CTGGGAAGCA TCATGAAAAT CGTGGTCAAC
    AACTTGGGAA TCGACGAAGC CTCCTGGATG AAGAGACTTG CAGTGTTCTC CCAACCCATT
    GTGAGCCGAG CCAAACCAGA GATGCTCAAA TCCCACTTCA TCCCCACCAT GGAGAAGCTG
    AAGAAGCGAA CCTCTAAAGT TGTAGCCGAG GAGGAGCATC TGCGTTTGGA GGGAAAGTCT
    GAGGGAGATG AGGAAGAGGG AACTATCAGA GACGAGTTTG CAGTTCTCTG TAGAGACCTC
    TATGCTCTAT ATCCTCTTCT CATCCGCTAT GTGGATAACA ACAGAGCAAG ATGGCTGACG
    TTTCGAGACC CAGATGCTGA GGAGTTGTTC CGCATGGTGG GAGAGGTCTT CATCTTCTGG
    TCAAAATCTC ACAATTTCAA GCGAGAGGAG CAAAACTTTG TGGTGATGAA CGAAATCAAC
    AATATGTCCT TTCTCACTGC TGACAGTAAG AGCAAGATGA GCAAGGCCGG GGACTCTGAG
    GCTGGAGGCT CGGATGTGGA GCGTACCAAG AAAAAGAGAC GGGGGGATCG ATACTCTGTG
    CAAACGTCCC TCATTGTGGC CGCACTGAAG AAAATGCTTC CCATTGGACT TAACATGTGT
    TCTCCTGCAG ACCAGGAGCT GATTAATCTG GCAAAGACAC GTTATTCTCT GAAAGACACA
    GATGAGGAGG TCAGAGAGTT CTTGCAAAAT AACCTGCACC TTCAGGGAAA GGTTGATAAC
    CCATCTATGA GGTGGCAGAT GGCACTGTAT AAGGAAATGG CAGGAAAGGC TGAAGATGCA
    GATGCCCCAG AGAATGTGGT CAAGAGGGTT CAGGAGGTCT CTGCGGTCCT TTACCACATT
    GAAGTGACGG AGCACCCTTT CAAGTCCAAA AAGATGGTTT GGCACAAACT GCTGTCGAAG
    CAGCGGCGTA GAGCGGTGGT GGCCTGTTTC AGGATGACTC CACTTTACAA CTTGCCCAGA
    CACAGAGCAT CTAATATGTT CCTGGATGGG TACAAGCGCA ACTGGATAAG AAATGAAGGC
    TATTCCTTTG AGGACAGAAT GATTGATGAC CTGTCTAAAG CATTGGAGGA GGAAGAAGAG
    GAGGAAGAGG AAAAGGAGAC AAAGCCAGAC CCTCTTCATC AGCTCATCCT TCACTTTAGC
    CGCACAGCAC TCACTGAGAA AAGTAAACTG GATATAGATC ACTTATACAT GGCATATGCT
    GATATCATGG CTAAGAGTTG CCACATGGAT GAAGAGGATG AGGGTGGAGA GGAGGAGAAT
    GAGGGTGGAG AGGAGGAGAT GTCCTTTGAG GAAAAGGAAA TGGAAAAGCA AAGACTGCTC
    TACCAGCAGT CGCGTCTACA CAACCGAGGG GCTGCTGAAA TGGTCCTGCA GATGATCAGT
    GCCTGCAAGG GTGAGACTGG TTGTATGGTC TCTTCCACCC TAAAACTTGG CATTTCTATT
    TTAAATGGCG GGAACTGTGA AGTTCAGCAG AAAATGCTGG ACTACCTGAA AGACAAGAAA
    GATGTGGGTT TCTTTCTCAG TGTTCAGGCT TTAATGCAGA CCTGCAGTGT TTTGGACCTG
    AATGCCTTTG AGAGGCAGAA TAAAGCTGAG GGTCTAGGTA TGGTGTCAGA AGAGGGTACA
    AGTGAGAAGG TCATGGCTGA TGACGAGTTC ACTTGCGACC TCTTCCGCTT TCTCCAGCTC
    CTCTGTGAGG GTCACAACAA TGACTTCCAA AATTACCTCA GAACACAGAC AGGCAGTACC
    ACCACCATCA ATGTCATTAT CTGTACAGTG GATTACCTGC TTAGACTACA GGAGTCCATC
    AGTGATTTCT ACTGGTATTA CTCTGGAAAA GACATCATAG ATGAACCAGG AAAGCGAAAC
    TTCTCTAAAG CTATGACTGT GGCCAAACAG GTTTTCAACA GTCTTACTGA GTATATCCAG
    GGTCCCTGCA CAGGAAACCA GCAGTCCCTG GCTCACAGTC GCTTATGGGA TGCTGTGGTG
    GGCTTTCTGC ACGTGTTTGC TCATATGATG ATGAAACTGG CTCAGGGAAG AGATTCCAGT
    CAGATTGGTC TTCTGAAAGA GTTGTTGGAC CTTCAGAAAG ACATGGTGGT CATGTTATTG
    TCCTTGTTGG AGGGTAATGT TGTAAATGGC ACCATTGCTC GCCAGATGGT GGACATGCTA
    GTGGAATCCT CCAGCAATGT AGAAATGATC CTTAAGTTCT TCGACATGTT CCTCAAGCTG
    AAGGACATTG TTGCGTCTGA CGCGTTTCGT GACTACGTGA CCGACCCTAG AGGTTTGATA
    TCCAAGAAAG ACTTTCAGAA ATCCATGGAC AGCCAGAAAC AGTACACACC TTCAGAGATC
    CAATTCCTAT TGTCATGCTC TGAGGCTGAT GAGAATGAGA TGATCAATTA CGAGGAGTTT
    GCAAGTCGCT TTCAAGAGCC GGCCAAAGAC ATTGGTTTTA ACATAGCGGT GCTGCTAACC
    AACCTCTCCG AACATGTTCC TCACGATACA AGACTGCAGA ACTTCCTCGA ACAAGCCGAG
    AGCGTCCTGA ACTATTTCCG CCCATTCTTG GGTCGCATCG AGATCATGGG CGCCAGCAGG
    AAAATCGAGA GAATCTACTT CGAGATCAGC GAGGTGAATC GCAAGCAGTG GGAGATGCCT
    CAAGTCAAAG AATCAAAGAG ACAGTTCATA TTCGATGTAG TCAATGAAGG CGGCGAATCG
    GAGAAGATGG AGCTTTTCGT CAACTTCTGC GAGGACACCA TATTCGAGAT GAACATTGCG
    TCGCAGATTT CAGAACAGGA CGAGGAGGAG AAAGAAGAGG ATGATATTGA GGAAGCTGAA
    GGAGGAGATG CTGGTGATGA AGAGGGGGGT GGCGAGGAGG GACAACCAGA ATCAAGCTCT
    GCATTTGCCG ATTTCATTCA AAGCATCATG AATTTTCTGG GTATGTTCAC CTTCAGGAAT
    ATTCGCAGAA AGTACCGCAG ACTCCGCAAG ATGACCATTA AGGAGATCGT GGTAGGGCTT
    GCTACCTTCA TGTGGACGAT TCTCTTGGGC ATCCTCCATT TCATTTACAG TGTTTGTAAA
    GGCTTCTTCC TCCTCATCTG GCACACGCTG TTTGGAGGAG GTTTAGTTGA AGGGGCCAAA
    AACATCACAG TAACTGAGTT ACTAGCCAGC ATGCCAGATC CCACACAGGA CGAGGTGCAT
    GGGGACTTAC CTGGAGAACC GAGGGGAGGA GAGGAACAGG AGACTGGAGG GATAATGGAC
    TCGATGGATG CTGGAGGGGG AGAGGAAGAG GATGAGGATA TCCAGGAGAA AGATGGTGGA
    AAGATTCCTG GTATAGATAC ACCTGGTGGA CTTGGAGATA TGGGTGATAC AACTCCTGTA
    GAACCGCCGA CTCCCGAGGG TACCCCTTTG TTGAAGAGGA AAGCGCAACC AGATGAAGCA
    GGAGCTGAAC AGCCTCCTGC TATTGAAGAG CCTCCTCCTG AGCCTGAGAA AGCAGACATT
    GAGAATGGTG AGAAGGCTGA GAAGGAAGTG GAGGTCAAGC CTGAGCCAAT ACCAGAAGAA
    CCGAAACCTC AGAGAGCCAC CAAAGCCAAG AAGGAGAAGA AATCAGTCAA GGGTGCAGGA
    TTTGAGCTCT GGAATGAGCT GGATGTGCAA AGAAATAAAT TCATGAACTG CTTGTCTCGT
    AACTTTTACA ACATTCGTTT CCTGGCTCTG TTCCTTGCCT TTGCCTTGAA CTTCATTCTT
    CTCTTCTACA AGGTATCTGA TACTCCCCCC AGTTCTGATG AATTTGAAGG TTCTGGCATC
    TTTGAAGGCT CTGGGCTGTT TGAAGGCTCT GCGGAGGATT TTGAGGGCTC TGGTGGAGAG
    GATGGAGGTG ATGATGAGGA TGAAGAAGGA CCTGTGTATT ATTTTCTGGA AGAAAGCACG
    GGATACATGC AGCCCACTCT GTCCTTCCTG GCCATTTTGC ACACCGTCAT TGCTTTCATA
    TCCATCATTG GGTACAACTG CCTCAAGATT CCACTGGTCA TCTTTAAGAG AGAGAAAGAG
    TTGGCGAGAA AGCTGGAGTT TGATGGCCTT TACATCACTG AACAGCCGGC AGATGATGAC
    ATTAAAGGCC AGTGGGACAG ACTGGTGTTG AACACTCCCT CTTTTCCCAC CAATTACTGG
    GACAAATTTG TGAAGCGAAA GGTTCTGGAT AAATATGGAG ATATTTATGG AAGAGAGAGA
    ATCGCAGAGC TGCTGGGTAT GGATCTGGCA TCTCTGGATG TCAGCAAACA ACAGACAGAC
    AAGAAACCAG AAGAGCCAGA CAACTCTATG CTTGCATGGT TCACTTCCAT TGACATCAAG
    TATCAGATCT GGAAATTTGG AGTTGTGTTC ACAGACAATA CTTTCCTCTA CCTGGTGTGG
    TACACAGTGA TGTCCCTTCT TGGACATTAC AACAACTTTT TCTTTGCTTG TCACCTGCTG
    GACATCGCAA TGGGTGTTAA GACCCTGCGT ACAATTCTTT CCTCCGTCAC ACATAATGGA
    AAACAGCTGA TGATGACTGT GGGTTTGCTG GCTGTGGTTG TGTACCTCTA CACTGTGGTG
    GCCTTCAACT TCTTCAGGAA GTTCTACAAT AAGAGCGAGG ATGAGGATGA GCCGGATATG
    AAGTGTGACG ACATGATGAC GTGTTATTTG TTCCACATGT ATGTGGGTGT ACGGGCAGGT
    GGAGGTATAG GAGATGAGAT CGAGGACCCT GCTGGAGATG AGTATGAGCT CTATCGGGTG
    GTCTTCGACA TCACCTTCTT CTTCTTTGTC ATCGTCATCC TGCTGGCCAT CATTCAGGGT
    CTGATCATTG ATGCCTTTGG TGAACTGAGA GACCAGCAAG AGCAAGTGAA AGAGGACATG
    GAGACCAAAT GCTTTATATG TGGCATTGGG AGCGACTACT TTGATACAAC GCCCCATGGC
    TTTGAGACCC ATACGCTTGA AGAACACAAC CTGGCCAATT ACATGTTTTT CCTGATGTAC
    TTAATCAACA AGGATGAAAC AGAGCACACT GGCCAGGAAT CATACGTGTG GAAGATGTAC
    CAGGAGAGAT GCTGGGATTT CTTCCCTGCA GGAGACTGCT TCAGAAAGCA ATATGAAGAC
    CAGCTTGGAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009303777.1
    CDS1..15192
    Translation

    Target ORF information:

    RefSeq Version XM_009305502.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio ryanodine receptor 1a (skeletal) (ryr1a), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009305502.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    13261
    13321
    13381
    13441
    13501
    13561
    13621
    13681
    13741
    13801
    13861
    13921
    13981
    14041
    14101
    14161
    14221
    14281
    14341
    14401
    14461
    14521
    14581
    14641
    14701
    14761
    14821
    14881
    14941
    15001
    15061
    15121
    15181
    ATGGCAGAAG GAGCTGAGGG AGAAGAGGAG ATCCAATTCC TAAGAACAGA TGACCAGGTG 
    GTGCTACAGT GTACAGCTAC TATCCTGAAG GAGCAGATTA AACTCTGTCT GTCCTGTGAG
    GGTTTTGGGA ATCGCCTGTG TTTTTTAGAA ACCACATCTA ATGCTCAGAA TGTTCCTCCT
    GATCTGGCAA TATGCTGCTT TATCCTGGAG CAGTCTTTAT CTGTGCGGGC CCTGCAGGAG
    ATGCTGGCTA ACACTACTGA GCTTAATGAG GCAGTCGATT TGGATAAATG GTCTTCACAA
    GGAGGAGGCC ACCGCACGTT GTTGTATGGC CACGCTATCC TACTCAGACA TACTCACTCT
    GGCATGTACC TTAGTTGCTT GACCACATCA AGATCCCTCA CAGACAAACT GGCCTTTGAT
    GTGGGCTTGC AGGAGGACTC CACAGGTGAG GCCTGTTGGT GGACCATTCA CCCTGCATCC
    AAGCAGAGGT CAGAGGGTGA GAAGGTCAGA GTGGGCGATG ACATTATTCT GGTCAGCGTG
    TCCTCTGAGA GATATCTGCA CCTCTCCTAT GCCAGCGGAG ACCTGATGGT GGACGCTTCT
    TTCATGCAGA CCCTTTGGAA CATGAACCCA GTCTGTTCAG GCTGTGAGCT GGCAGAGGGG
    TTTCTGACTG GAGGGCATGT TCTTCGTCTA TTTCACGGGC ACATGGATGA GTGTTTAACA
    ATTCCTGGCG CGGAGCAGGG CGACGATCAG AGACGAAATG CTCACTATGA AGGTGGAGCC
    GTGTGCAGCC ATGCTAGATC TCTCTGGAGA CTGGAGCCTC TTCGCATTGG ATGGAGTGGT
    AGCCACATGA AGTGGGGTCA GTCATTCAGG GTGCGCCACA TCACAACAGG CAGGTATCTG
    TGTCTGGACG AAGAGAAAGG ACTCCTGATT GTGGACCCAG AAAAGGCAAA TACGAAGCAG
    TCAGCTTTCT GCTTTCGTGC ATCAAAGGAG AAGACAGAAG TAGCTCAGAA ACGTGATGTG
    GAGGGAATGG GGATCCCAGA GATCAAGTAT GGCGAGTCCA TGTGCTTTGT GCAGCACGTC
    GCTACAAGTC TGTGGCTCAC ATATGCCTCG GTGGATGCCA AGTCTGCTCG GCTGGGACCA
    CTGAAGAGGA AGGCTATCCT GCATCAGGAG GGTCACATGG ATGATGCTTT GACTGTGGCT
    CGCTCTCAGA CAGCTGAATC GCAGGCTGCC AGGATGATCT ACAACACGGC AGGACTTTTC
    ACTCAGTTCA TTAAGGGTTT GGACTCTCTA AGTGGGAAGA GTAAATCTCC AGGACCTTCC
    CCTTCTCTGC CTCTAGACGC CATAGTTCTG TCATTGCAGG ATCTCATCTT CTATTTCCGA
    CCTCCAGAAG AGGAGCTGGA ACATGAGGAG AAGCAGACCA AACTGCGCTC CTTAAGAAAC
    CGCCAGAATC TCTTCCAAGA GGAGGGCATG ATCACTCTTG TATTGGACTG TGTTGATCGG
    TTGAATGTCT ACAACACTTC AGCCCACTTC TCAGAATTTG CTGGAGAGGA CGCAGCCGAG
    TCGTGGAAAG AGATCGTCAA CTTATTTTAT GAGCTGCTGG CATCTCTGAT CAGAGGAAAC
    AGGGCAAACT GTGCTTTGTT CTGTGATAAT CTTGACTGGT TGGTCAGCAA ACTGGATCGT
    CTGGAGGCAT CCTCGGGTAT TCTGGAGGTG CTTTATTGTA TCCTAATTGA AAGCCCTGAG
    GTGCTTAACA TCATCCAGGA AAACCACATC AAATCTATCA TTGCACTTTT GGACAAACAT
    GGACGCAATC ACAAGGTTTT AGATGTTCTC TGCTCATTAT GCGTGTGTAA TGGTGTTGCT
    GTGAGGTCCA ATCAGAATCT GATCACTGAA AACCTGCTTC CCGGACGGGA TCTTCTCCTT
    CAGACCAACA TCATTAATTA TGTTACCAGT ATGAGACCCA ACATTTTTCT GGGCACGTGT
    GAAGGCTCGA CTCAGTATAA GAAGTGGTAC TATGAGTTGA TCGTTGACCA TGTGGAGTCT
    TTCCTTACCG CTCATCCATT TCACCTTCGT GTTGGCTGGG CTTTGACGGA GGGCTACAGC
    CCGTATCCAG GAGGTGGAGA AGGATGGGGA GGCAATGGAG TTGGAGATGA TCTACACTCG
    TATGGTTTCG ATGGGTTGAA TCTGTGGACA GGTCGTGTAC CACGTCAGGT GACGTCACCC
    AGTCAACACA TTCTCTCGGC TGAGGATGTG GTGAGCTGCT GTTTAGATCT AAGCGTTCCC
    AGCATCTCCT TTCGTATTAA TGGACATCCC GTTCAAGGCA TGTTCGAGAA CTTCAATCTG
    GATGGTCTTT TCTTCCCCGT GGTCAGCTTC TCAGCGGGGA TAAAAGTTCG TTTTCTCCTT
    GGCGGACGAC AAGGAGACTT CAAATTCCTC CCTCCTCCAG GTTATGCCCC GTGCTATGAG
    GCCGTTCTGC CCAAAGACAG ACTGCGGATT GAACCCATTA AAGAGTACAA GCACGACAAC
    GACGGTGTCC GAAACCTGCT GGGACCGACA CAGTCTCTTT CCCACACCGC CTTCACTCCC
    TGTCCGGTGG ACACTGTGCA GATTGTGCTT CCTCCACATC TAGAGCGCAT CAGAGAGAAA
    CTGGCCGAAA ATAGCCATGA GCTGTGGGCC GTCACTCGAA TTGAACAGGG TTGGACATAT
    GGACTATTTC GTGATGACAA CAAGAAGCTG CATCCCTGTC TAGTGGACTT CCAGAGCCTC
    CCGGATCCTG AGAAGAGCTA CAACCTGGCC ATGTCTGGAG AGACGCTGAA GACTCTTCTG
    GCTTTAGGGT GTCATGTAGG CATGGGTGAT GAAAAAGCAG AGGAGAACCT GAAGAAGATC
    AAACTTCCTA AAACGTATAT GCAGTGTAAC GGATACAAAC CTGCTCCACT GGATCTTAAC
    CATGTGAAGC TCACACCGAA TCAGAACACA CTTGTGGAAA GGCTGGCTGA AAATGGACAC
    AACGTCTGGG CTCGAGACCG TGTCCGCCAA GGGTGGACTT ACAGCATCAT TCAGGATATT
    GTGAACAAGC GCAACCCTCG TCTCGTGCCT TACATTCTGC TTGATGAAAA GACAAAAAAG
    ACCAATCGTG ACACTGTGTG TGCCGCTGTA CGGACTCTGA TTGGCTATGG CTACAATATT
    GAACCCCCTG ACCAGGAGAG CAGTGGTCAT GGGGGTGGCA ATGGCCGGAG TGATAAGATC
    CGTGTGTTCA GGGCAGAGAA ATCCTACGCA GTGACGCAGG GTAAATGGTA CTTTGAGTTC
    GAGGCAGTAA CAGTGGGAGA GATGAGAGTT GGGTGGGCAA GACCAAGCGT CCATGCAGAC
    AGAGAGCTGG GATCAGATGA CCTGGCCTAC GTGTTCAATG GCTTTAAGTC TCAGCGCTGG
    CACATTGGAA ATGAGCCGTT TGGCCGACAG TGGCAGTCTG GTGATGTCGT TGGCTGCATG
    ATAGACCTGA CGGAGCAGAA CATCATGTTT ACTTTGAATG GAGAGATGCT CATCAGTGAC
    TCTGGATCTG AGATGGCATT TAAAGACATT GAGATCGGAG AAGGCTTCAT CCCTGTATGC
    AGTCTGGGTA TGTCCCAGGT GGGTCGGATC AACCTTGGTC AAAATGTGAG CAGCCTGCGG
    TACTTTACAA TCTGTGGACT GCAGGAAGGT TTCGAGCCAT TTGCTATCAA CATGAAGAGA
    GATATTACAA TGTGGTTCAG CAAGAGCTTG CCCCAGTTTG TGCCCGTTCC AACAGATCAT
    CCTCATATTG AGGTGTCCCG TGTGGATGGA ACGGTGGAGA GCGCTCCCTG TCTGAAACTG
    ACTCACAGGA CATTCGGTTC TCAGAATGCC AACACAGACC TGTTGTTCTT CAGACTCAGT
    ATGCCGATTG AATTCCACGA AACCTTCAAG GTCCCGGTGG GAGCTTCACC CCTCACACGA
    ACCCTCACTA TCCCTGAGGA TGAGACTCTT GAGGTGGATC CCGAATCTGA ATTCGAGCTC
    CTGAAGAAGT CAGCCACTCG TAAGGAGCAA GATGAGAAAG AAAAAGAGCC GTCCGTCCCG
    AAAGAGCTGC CTGGAAGCAA AGAAAGCGAG AATGAGAAGG ACACCACCAC AGAGAAGAGC
    AAAAAGAGAG GATTTTTTTC CAAGGCGAAG AAGGCAGCGT TTATTGCCCC ACCACCAGTG
    GTTCCAACAA TGCCTCGTCT AATGGAGGAT GTTGTCCCAG ATGATCGAGA CGATGTTGAA
    ATTATCTCAA ACACAACAAC TTATTATTAT TCAGTCCGGG TGTTCGCCGG ACAGGAGCCC
    AGTGGAGTAT GGGTAGGCTG GGTGACTCCT GATTACCACT TGTACGACCT TCACTTTGAT
    CTTTGTAAAG TCAGAAACGT AACTGTTACA GTTGGAGACG ATAAAGGCAA TATTCATGAC
    AGTGTGAAAC GCAGTAATTG CTACATGGTG TGGGGTGGAG AGTTTAGCAG CTCACAGCAA
    ACCCGCATCA GTCAGGAGGA CTTTGTGATC GGTTGTTTGG TGGACCTGGA CACAGGACTG
    ATGACCTTTA CAGCCAATGG GAAAGAGATC AATACGTTCT ATCAGGTTGA ACCTAACACT
    AAGCTTTTCC CTGCTGTGTT TTGTTTGCCA TCAAGTCAGA ACATGTTACA AGTGGAACTC
    GGCAAACTGA AGAACATTAT GCCCATCTCA GCAGCCATGT TTCGCAGCGA CTGTAAGAAC
    CCGGTCCCTC AATGCCCTCC CAGGTTAGAT CTTCAGATGC TGACCCCTGT CATATGGAGC
    CGGATGCCTA ATCACTTTTT GTCGCCTGAA ACTGGCCGCG TGAGTGAGAG GCAGGGCTGG
    AAAGTGCAAT GCATGGAACC TCTCGTTATG ATGGCACTGC ACATCCCTGA AGAGAACAGG
    TGTATTGACA TCCTGGAACT GTCAGAACGT ACCGACCTGA TGAAGTTCCA TTACCACACA
    CTGAAACTGT ATGGCTCGGT TTGCGCTCTG GGGAACAATC GTGTCGCACA CGCCCTCTGC
    AGCCACGTGG ACGAATCGCA GCTCTTTTAC GCCATCGAAA ACACGTACCT GCCTGGCCCG
    ATCCGCAGTG GCTACTACGA CCTGCTCATC TCCATGCACC TAGAGTCTGC CAAACGCAAC
    CGACTCATGA CCAATAAGGA GTTCATTGTG CCCATGACGG ACGAGACTCG AAGCATCACC
    CTTTATTCGG ATGCTGAGAA GGCACATGCG TTACCTGGTG TCGGACTTAC CACATGTCTA
    CGCCCAAAAC TTCACTTTGC TCCAACTGGA TTTGTGGGCA CTAATGCTGA CCTTTACACC
    CTGAGCCCCA TGTTACCTCT TCAGATGCTT AAAGATCATG CCCTCAGCAT GCTTACAGAA
    GCTGTGCAGG ACGGTGGCCA AGCCATTCGA GATCCTGTAG GGGGCAGCGT TGAGTACCAC
    TTTGTGCCCA TCCTGAAGCT CATCAGCACC CTTCTCATTA TGGGTGTATT TGATGACGAC
    GATGTCAAGC ACATCCTGAA ACTGATCGAA CCATCCGTCT TCAGCAGCAA CAGTGAGACT
    CCAGCTGAAG AAACTGGAGA TCCTGAACAG CAGCTGGTCT GCAAAGAGGA AGGAGCGGAG
    GTAAAAGAGC AAGAGGAACC AGCAGACGGT GGAAATGATG GAGAGCTGCT GGATGAAGGG
    ATGGGAGAGG AAGAAGAGCT TGAGGAAGAG GAGGAGGAGG AAGATGAAGA GTATGAGGAG
    GAGGTGAAAA AAGAGGAGGA TGAACATGCT GCTGAAAAAA CAGACAGAGA GAAAACTACA
    GATGGAGAAA CTGAGAAGGA AACTGGTGCA GCAGAGGCTG AAGTAAGGGA GGAGGAGGAT
    GTTGGAGAAG GACTGCTGCA CATGAAGCTT CCAGAGTCTG TCAAACTCCA GATGTGTACT
    CTCCTGCAAT ATTTCTGTGA CTGTGAGCTG CGTCACAGAG TGGAAGCCAT CATCGCTTTC
    TCAGACAAGT TTGTTAACCA AGTGCAGACC AATCAGAGAC AGCGATATAA TGAGCTCATG
    CAAGCTTTTA CAATGAGTGC AGCAGAAACG GCCCGTAAGA CCCGCGAATT CCGCTCCCCT
    CCACAGGAAC AGGTGAACAT GTTGATGAAC TTTAAGAACT GTCCAGAGGA AGAAGATTGC
    CCCGTTCCAG AGGATGTCCG GAATGGCCTC CTGACATTTC ACCATGACCT TTTAGCTCAC
    TGTGGTGTGC ACATTGAAGA GGAGGAACAG GAAGAAGAGG TGGACACTTC TCTCAGTGGG
    CGCCTACGCA GACTGGTGGA AAAAGTTAAA AGCTTGCGAA AAAAGCCAGA AGCTGAGCCT
    GAGCCTGAAG AAGATAAAAA ACCAAGTACT CTGCAGGAGC TGATCTCCCA CACCATGATC
    TACTGGGCTC AAGAGTCTTT CATCCAGAAC CCTGAGCTGG TGCGCCTCAT GTTTAGTCTG
    CTACACCGGC AGTATGATGC TTTGGGTGAG CTGATTCGAG CTTTGCCCAA AGCCTATATC
    ATTAATGCTG TTTCTATACA AGACACAATG GAGCTCCTGG AGTGTCTGGG ACAGATACGC
    TCCTTGCTCA TCGTTCAGAT GGGACCAGAG GAAGAGAGGC TGATGATCCA GAGTATCGGA
    AATATTATGA GTAACAGGGT ATTTTACCAG CACCCCAACT TGATGAGAGC TCTGGGAATG
    CATGAGACAG TCATGGAGGT TATGGTGAAC GTACTCGGAG GCGGAGATTC CAAGGAGATC
    AGATTCCCTC GAATGGTTAC CAACTGCTGT CGTTTCCTGT GTTATTTCTG TCGTATCAGC
    AGACAGAATC AGCGCTCGAT GTTTGATCAT CTGGGCTACC TCCTGCAGAA CAGTGGCATT
    GGGCTGGGAA TGCGTGGCTC CACTCCTCTT GATGTTGCTG CTGCTTCCTG TATCGATAAT
    AATGAGCTTG CCCTGGCTCT CCAAGAGCAA GACCTGGAGA TGGTAGTAAA GTACCTGGCA
    GGCTGTGGTC TGCAGAGCTG CCCTATGCTT TTGTCAAAGG GTTACCCGGA TATTGGATGG
    AACCCAGTTG GAGGAGAAAA GTATCTGGAT TTCCTGCGCT TTGCTGTGTT TGTGAATGGA
    GAGAGTGTGG AGGAGAACGC TAATGTGGTG GTCCGGCTGC TGATCAGGAG GCCTGAGTGC
    TTTGGTCCTG CACTGAGAGG AGAAGGGGGC AATGGTCTGC TGGCTGCCAT TGAAGAAGCC
    ATCAAGATCT CAGAGGACCC TGCAAGAGAT GGACCCACTG TTAAAAAAGA CAGACGCTTC
    CCGATGTTTG GAGGTGAAGA GCAGCATGAG GAGAACAGAG TGCACCTAGG AAATGCCATC
    ATGTCATTTT ACTCAGCTCT TATTGATCTG CTGGGTCGCT GTGCTCCTGA GATGCATTTG
    ATCCAGGCTG GTAAAGGTGA AGCTTTGAGA ATCAGGGCTA TTCTTCGGTC GCTTGTGCCC
    ATTGAGGATC TGGTCGGTGT GATCAGCTTG TCAGTCCAGA TTCCTGCTTT TGGAAAAGAT
    AACAGCATTA TTGAGCCGAA GATGTCTGCT AGTTTTGTTC CTGATCATAA AGCACCAATG
    GTCCTGTTCC TGGACAGAGT TTATGGCATC GACAACCAGG ACTTCCTATT GCATGTACTA
    GAAGTGGGTT TCCTGCCTGA TATGAGGGCG GCAGCCTCCT TAGACACAGC TGCATTCAGC
    ACTACAGAAA TGGCCTTGGC TCTGAACCGT TACCTGTGCT CCGCCGTGCT TCCTCTCATC
    ACTAAATGTG CTCCTTTGTT CGCCGGGACG GACCACCGAG CCATCATGAT CGACTCCATG
    CTTCACACCA TCTACAGGCT GTCTCGAGGG CGCTCGCTAA CGAAAGCACA AAGAGATGTT
    ATTGAGGAAT GCCTCATGTC TTTGTGCAGG TATCTGCGCC CTTCCATGCT CCAGCACCTT
    CTCAGGCGGC TAGTATTTGA TGTGCCTATT CTGAATGAGT ATGCCAAAAT GCCCCTTAAG
    CTGCTTACCA ATCATTATGA GCGTTGTTGG AAATATTATT GCTTGCCTAA TGGCTGGGCT
    AACTTTGGCG TATCATCAGA AGAGGAGCTT CATCTGACCC GTAAACTCTT TTGGGGGATC
    TTTGAATCTC TGGCTCACAA GAAATTCGAT GCAGAGCTGT TCAAAATCGC CATGCCTTGT
    ATATGTGCGA TCGCTGGTGC AATTCCTCCT GATTATGTGG ACGCCAGCTA CTCCTCCAAA
    ACCGAAAAAA AGGCTTCTGT TGATGCTGAA GGGAACTTTG ACCCCAAACC TGTTGATACC
    ACTAACACTA TAATTCCCGA GAAGTTGGAC GGGTTCATCA ACAGATATGC AGAATATACG
    CACGACAGAT GGGCTTTTGA AAAAATTCAA AACAACTGGA CATTTGGTGA GGTGATGGAT
    GAAAATGCCA AGACTCATCC CATGCTCAGA CCTTACAAAA CATTCTCTGA AAAGGATAAA
    GAAATCTACC GCTGGCCTAT TAAAGAGTCC ATCAAGGCCA TGATTGCCTG GGAGTGGACA
    CTGGACAAAG CCAGAGATGG AGAGGATGAG AAAGTTGAGA AGAAAACAGC CCGTAAGATC
    TCACAGACTG CACAGGCAAC CTATGACCCC AGTCATGGCT ATAGCCCCCA ACCTATTGAT
    ATCTCAGGCA TGACACTGTC CAGAGAGCTA CAGTCCATGG CAGAGCAGCT CGCAGAAAAC
    TACCACAACA CCTGGGGCCG TAAGAAGAAA ATGGAATTAC AAGCAAAAGG AGGAGGTACA
    CACCCTTTGC TTGTGCCTTA TGACACACTG ACGGCTAAAG AAAAGGCACG AGATAGAGAG
    AAAGCGTATG AGCTACTCAA GTTTCTGCAG CTCAATGGAT ATGCTGTGAC AAGAGGGCTC
    AAAGACATGG AAAGTGACAT TTCATCTATC GAGAAGCGTT TTGCGTATGG CTTCCTACAG
    AAGCTTCTGA AGTGGATGGA CATTGCTCAG GAGTTCATAG CACATCTTGA GGCGGTTGTG
    AGCAGCGGTC GTGTGGAAAA GTCACCACAT GAGCAAGAAA TCAAGTTCTT TGCCAAGATT
    CTTTTGCCTT TGATAAATCA GTATTTTAAA AACCACTGCC TTTACTTCCT CTCCACACCG
    GCCAAAATCC TAGGCAGCGG CGGACACGCC TCAAACAAGG AAAAGGAGAT GATTGCAAGC
    ATTTTCTGTA AGATGTCTGC CCTGGTGCGA CACAGAGTGT TTCTCTTTGG CACGGATGCG
    CCTGCTATTG TCAACTGCCT TCATATCCTC GCACGTTCGC TTGATGCCAG GACGGTGATG
    AAATCCGGGC CTGAGATTGT GAAAGCAGGA CTGCGTTTGT TCTTTGAGAG TGCAGCTGAT
    GACATTGAAA AGATGGTGGA GAATCTCAAA CTTGGCAAAG TGTCCAAAGG CAATCAGCCA
    GTGAAAGGTG TGTCCCAGAA TATAAACTAC ACCACAACGG CTCTGCTTCC TGTCCTCACA
    TCACTGTTTG ACCACATCGC TCAACACCAA TTCGGAGATG ATGTTATCCT GGATGATTTG
    CAGATGTCCT GCTACCGAAT CATGTGTAGC ATCTACTCTC TTGGGACTGT AAAAACTCCA
    CATGCAGAGA GGCATAGACC AGCCCTTGGA GAATGTCTCG CTCATCTCGC TGCAGCAATG
    CCTGTTGCCT ACCTGGAGCC GCATCTTAAT GAATACAACG CCTTTTCAGT CTACACTACC
    AAGACACCGA GAGAAAGAGC TATCCTTGGC CTTCCAAACC AAGTTCAAGA GTTGTGTTTG
    GACATTCCTG AGCTTGACGT TTTACTGAAG GAGATCGGAG ACCTGGCTGA ATCAGGAGCT
    CGATACACTG AAATGCCACA TGTGATTGAG GTCACTCTGC CCATGCTGTG TAACTATCTG
    CCCCGCTGGT GGGAGAGAGG ACTGGAGAAC TTCCCGGATC TAGAGGGTAA GCTGTGCACC
    GACGTCACCT CCGAATCCCT CAACCAGCTT CTGGGAAGCA TCATGAAAAT CGTGGTCAAC
    AACTTGGGAA TCGACGAAGC CTCCTGGATG AAGAGACTTG CAGTGTTCTC CCAACCCATT
    GTGAGCCGAG CCAAACCAGA GATGCTCAAA TCCCACTTCA TCCCCACCAT GGAGAAGCTG
    AAGAAGCGAA CCTCTAAAGT TGTAGCCGAG GAGGAGCATC TGCGTTTGGA GGGAAAGTCT
    GAGGGAGATG AGGAAGAGGG AACTATCAGA GACGAGTTTG CAGTTCTCTG TAGAGACCTC
    TATGCTCTAT ATCCTCTTCT CATCCGCTAT GTGGATAACA ACAGAGCAAG ATGGCTGACG
    TTTCGAGACC CAGATGCTGA GGAGTTGTTC CGCATGGTGG GAGAGGTCTT CATCTTCTGG
    TCAAAATCTC ACAATTTCAA GCGAGAGGAG CAAAACTTTG TGGTGATGAA CGAAATCAAC
    AATATGTCCT TTCTCACTGC TGACAGTAAG AGCAAGATGA GCAAGGCCGG GGACTCTGAG
    GCTGGAGGCT CGGATGTGGA GCGTACCAAG AAAAAGAGAC GGGGGGATCG ATACTCTGTG
    CAAACGTCCC TCATTGTGGC CGCACTGAAG AAAATGCTTC CCATTGGACT TAACATGTGT
    TCTCCTGCAG ACCAGGAGCT GATTAATCTG GCAAAGACAC GTTATTCTCT GAAAGACACA
    GATGAGGAGG TCAGAGAGTT CTTGCAAAAT AACCTGCACC TTCAGGGAAA GGTTGATAAC
    CCATCTATGA GGTGGCAGAT GGCACTGTAT AAGGAAATGG CAGGAAAGGC TGAAGATGCA
    GATGCCCCAG AGAATGTGGT CAAGAGGGTT CAGGAGGTCT CTGCGGTCCT TTACCACATT
    GAAGTGACGG AGCACCCTTT CAAGTCCAAA AAGATGGTTT GGCACAAACT GCTGTCGAAG
    CAGCGGCGTA GAGCGGTGGT GGCCTGTTTC AGGATGACTC CACTTTACAA CTTGCCCAGA
    CACAGAGCAT CTAATATGTT CCTGGATGGG TACAAGCGCA ACTGGATAAG AAATGAAGGC
    TATTCCTTTG AGGACAGAAT GATTGATGAC CTGTCTAAAG CATTGGAGGA GGAAGAAGAG
    GAGGAAGAGG AAAAGGAGAC AAAGCCAGAC CCTCTTCATC AGCTCATCCT TCACTTTAGC
    CGCACAGCAC TCACTGAGAA AAGTAAACTG GATATAGATC ACTTATACAT GGCATATGCT
    GATATCATGG CTAAGAGTTG CCACATGGAT GAAGAGGATG AGGGTGGAGA GGAGGAGAAT
    GAGGGTGGAG AGGAGGAGAT GTCCTTTGAG GAAAAGGAAA TGGAAAAGCA AAGACTGCTC
    TACCAGCAGT CGCGTCTACA CAACCGAGGG GCTGCTGAAA TGGTCCTGCA GATGATCAGT
    GCCTGCAAGG GTGAGACTGG TTGTATGGTC TCTTCCACCC TAAAACTTGG CATTTCTATT
    TTAAATGGCG GGAACTGTGA AGTTCAGCAG AAAATGCTGG ACTACCTGAA AGACAAGAAA
    GATGTGGGTT TCTTTCTCAG TGTTCAGGCT TTAATGCAGA CCTGCAGTGT TTTGGACCTG
    AATGCCTTTG AGAGGCAGAA TAAAGCTGAG GGTCTAGGTA TGGTGTCAGA AGAGGGTACA
    AGTGAGAAGG TCATGGCTGA TGACGAGTTC ACTTGCGACC TCTTCCGCTT TCTCCAGCTC
    CTCTGTGAGG GTCACAACAA TGACTTCCAA AATTACCTCA GAACACAGAC AGGCAGTACC
    ACCACCATCA ATGTCATTAT CTGTACAGTG GATTACCTGC TTAGACTACA GGAGTCCATC
    AGTGATTTCT ACTGGTATTA CTCTGGAAAA GACATCATAG ATGAACCAGG AAAGCGAAAC
    TTCTCTAAAG CTATGACTGT GGCCAAACAG GTTTTCAACA GTCTTACTGA GTATATCCAG
    GGTCCCTGCA CAGGAAACCA GCAGTCCCTG GCTCACAGTC GCTTATGGGA TGCTGTGGTG
    GGCTTTCTGC ACGTGTTTGC TCATATGATG ATGAAACTGG CTCAGGGAAG AGATTCCAGT
    CAGATTGGTC TTCTGAAAGA GTTGTTGGAC CTTCAGAAAG ACATGGTGGT CATGTTATTG
    TCCTTGTTGG AGGGTAATGT TGTAAATGGC ACCATTGCTC GCCAGATGGT GGACATGCTA
    GTGGAATCCT CCAGCAATGT AGAAATGATC CTTAAGTTCT TCGACATGTT CCTCAAGCTG
    AAGGACATTG TTGCGTCTGA CGCGTTTCGT GACTACGTGA CCGACCCTAG AGGTTTGATA
    TCCAAGAAAG ACTTTCAGAA ATCCATGGAC AGCCAGAAAC AGTACACACC TTCAGAGATC
    CAATTCCTAT TGTCATGCTC TGAGGCTGAT GAGAATGAGA TGATCAATTA CGAGGAGTTT
    GCAAGTCGCT TTCAAGAGCC GGCCAAAGAC ATTGGTTTTA ACATAGCGGT GCTGCTAACC
    AACCTCTCCG AACATGTTCC TCACGATACA AGACTGCAGA ACTTCCTCGA ACAAGCCGAG
    AGCGTCCTGA ACTATTTCCG CCCATTCTTG GGTCGCATCG AGATCATGGG CGCCAGCAGG
    AAAATCGAGA GAATCTACTT CGAGATCAGC GAGGTGAATC GCAAGCAGTG GGAGATGCCT
    CAAGTCAAAG AATCAAAGAG ACAGTTCATA TTCGATGTAG TCAATGAAGG CGGCGAATCG
    GAGAAGATGG AGCTTTTCGT CAACTTCTGC GAGGACACCA TATTCGAGAT GAACATTGCG
    TCGCAGATTT CAGAACAGGA CGAGGAGGAG AAAGAAGAGG ATGATATTGA GGAAGCTGAA
    GGAGGAGATG CTGGTGATGA AGAGGGGGGT GGCGAGGAGG GACAACCAGA ATCAAGCTCT
    GCATTTGCCG ATTTCATTCA AAGCATCATG AATTTTCTGG GTATGTTCAC CTTCAGGAAT
    ATTCGCAGAA AGTACCGCAG ACTCCGCAAG ATGACCATTA AGGAGATCGT GGTAGGGCTT
    GCTACCTTCA TGTGGACGAT TCTCTTGGGC ATCCTCCATT TCATTTACAG TGTTTGTAAA
    GGCTTCTTCC TCCTCATCTG GCACACGCTG TTTGGAGGAG GTTTAGTTGA AGGGGCCAAA
    AACATCACAG TAACTGAGTT ACTAGCCAGC ATGCCAGATC CCACACAGGA CGAGGTGCAT
    GGGGACTTAC CTGGAGAACC GAGGGGAGGA GAGGAACAGG AGACTGGAGG GATAATGGAC
    TCGATGGATG CTGGAGGGGG AGAGGAAGAG GATGAGGATA TCCAGGAGAA AGATGGTGGA
    AAGATTCCTG GTATAGATAC ACCTGGTGGA CTTGGAGATA TGGGTGATAC AACTCCTGTA
    GAACCGCCGA CTCCCGAGGG TACCCCTTTG TTGAAGAGGA AAGCGCAACC AGATGAAGCA
    GGAGCTGAAC AGCCTCCTGC TATTGAAGAG CCTCCTCCTG AGCCTGAGAA AGCAGACATT
    GAGAATGGTG AGAAGGCTGA GAAGGAAGTG GAGGTCAAGC CTGAGCCAAT ACCAGAAGAA
    CCGAAACCTC AGAGAGCCAC CAAAGCCAAG AAGGAGAAGA AATCAGTCAA GGGTGCAGGA
    TTTGAGCTCT GGAATGAGCT GGATGTGCAA AGAAATAAAT TCATGAACTG CTTGTCTCGT
    AACTTTTACA ACATTCGTTT CCTGGCTCTG TTCCTTGCCT TTGCCTTGAA CTTCATTCTT
    CTCTTCTACA AGGTATCTGA TACTCCCCCC AGTTCTGATG AATTTGAAGG TTCTGGCATC
    TTTGAAGGCT CTGGGCTGTT TGAAGGCTCT GCGGAGGATT TTGAGGGCTC TGGTGGAGAG
    GATGGAGGTG ATGATGAGGA TGAAGAAGGA CCTGTGTATT ATTTTCTGGA AGAAAGCACG
    GGATACATGC AGCCCACTCT GTCCTTCCTG GCCATTTTGC ACACCGTCAT TGCTTTCATA
    TCCATCATTG GGTACAACTG CCTCAAGATT CCACTGGTCA TCTTTAAGAG AGAGAAAGAG
    TTGGCGAGAA AGCTGGAGTT TGATGGCCTT TACATCACTG AACAGCCGGC AGATGATGAC
    ATTAAAGGCC AGTGGGACAG ACTGGTGTTG AACACTCCCT CTTTTCCCAC CAATTACTGG
    GACAAATTTG TGAAGCGAAA GGTTCTGGAT AAATATGGAG ATATTTATGG AAGAGAGAGA
    ATCGCAGAGC TGCTGGGTAT GGATCTGGCA TCTCTGGATG TCAGCAAACA ACAGACAGAC
    AAGAAACCAG AAGAGCCAGA CAACTCTATG CTTGCATGGT TCACTTCCAT TGACATCAAG
    TATCAGATCT GGAAATTTGG AGTTGTGTTC ACAGACAATA CTTTCCTCTA CCTGGTGTGG
    TACACAGTGA TGTCCCTTCT TGGACATTAC AACAACTTTT TCTTTGCTTG TCACCTGCTG
    GACATCGCAA TGGGTGTTAA GACCCTGCGT ACAATTCTTT CCTCCGTCAC ACATAATGGA
    AAACAGCTGA TGATGACTGT GGGTTTGCTG GCTGTGGTTG TGTACCTCTA CACTGTGGTG
    GCCTTCAACT TCTTCAGGAA GTTCTACAAT AAGAGCGAGG ATGAGGATGA GCCGGATATG
    AAGTGTGACG ACATGATGAC GTGTTATTTG TTCCACATGT ATGTGGGTGT ACGGGCAGGT
    GGAGGTATAG GAGATGAGAT CGAGGACCCT GCTGGAGATG AGTATGAGCT CTATCGGGTG
    GTCTTCGACA TCACCTTCTT CTTCTTTGTC ATCGTCATCC TGCTGGCCAT CATTCAGGGT
    CTGATCATTG ATGCCTTTGG TGAACTGAGA GACCAGCAAG AGCAAGTGAA AGAGGACATG
    GAGACCAAAT GCTTTATATG TGGCATTGGG AGCGACTACT TTGATACAAC GCCCCATGGC
    TTTGAGACCC ATACGCTTGA AGAACACAAC CTGGCCAATT ACATGTTTTT CCTGATGTAC
    TTAATCAACA AGGATGAAAC AGAGCACACT GGCCAGGAAT CATACGTGTG GAAGATGTAC
    CAGGAGAGAT GCTGGGATTT CTTCCCTGCA GGAGACTGCT TCAGAAAGCA ATATGAAGAC
    CAGCTTGGAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.