DENND3A cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	DENND3A
Entrez Gene ID	797970
Full Name	DENN/MADD domain containing 3a
General protein information	Preferred Names DENN/MADD domain containing 3a Names DENN domain-containing protein 3
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005169976.3	XP_005170033.1	DENN domain-containing protein 3 isoform X1
XM_009293932.2	XP_009292207.1	DENN domain-containing protein 3 isoform X2
XM_021468228.1	XP_021323903.1	DENN domain-containing protein 3 isoform X1
XM_021468227.1	XP_021323902.1	DENN domain-containing protein 3 isoform X1
XM_001338383.6	XP_001338419.4	DENN domain-containing protein 3 isoform X1
XM_001338383.7	XP_001338419.5	DENN domain-containing protein 3 isoform X2
XM_005169977.3	XP_005170034.1	DENN domain-containing protein 3 isoform X3

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Dennd3	XP_235398.6
Bos taurus (cattle)	DENND3	XP_002692618.1
Xenopus tropicalis (tropical clawed frog)	dennd3	XP_002934521.2
Canis lupus familiaris (dog)	DENND3	XP_003431853.1
Danio rerio (zebrafish)	dennd3a	XP_001338419.4
Pan troglodytes (chimpanzee)	DENND3	XP_519983.3
	DENND3	XP_001082457.1
Mus musculus (house mouse)	Dennd3	NP_001074535.1
Homo sapiens (human)	DENND3	NP_055772.2
Gallus gallus (chicken)	DENND3	XP_418419.2

Gene Ontology

The following DENND3A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DENND3A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa23721	XM_005169977.3 Latest version!	Danio rerio DENN/MADD domain containing 3a (dennd3a), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
ODa150189	XM_021468228.1 Latest version!	Danio rerio DENN/MADD domain containing 3a (dennd3a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
ODa150189	XM_021468227.1 Latest version!	Danio rerio DENN/MADD domain containing 3a (dennd3a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
ODa150190	XM_001338383.7 Latest version!	Danio rerio DENN/MADD domain containing 3a (dennd3a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
ODa23720	XM_001338383.6 Latest version!	Danio rerio DENN/MADD domain containing 3a (dennd3a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
ODa23720	XM_005169976.3 Latest version!	Danio rerio DENN/MADD domain containing 3a (dennd3a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
ODa47230	XM_009293932.2 Latest version!	Danio rerio DENN/MADD domain containing 3a (dennd3a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DENND3A ( XM_005169977.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005170034.1 Danio rerio DENN/MADD domain containing 3a (dennd3a), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ODa23721
Clone ID Related Accession (Same CDS sequence)	XM_005169977.3
Accession Version	XM_005169977.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2307bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	DENN domain-containing protein 3 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007130.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005169977.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGATA ATGTGCCGTG TGGTCTGCTG GAGGCCTGTG TGCTGCTTGG AGTGTCAAAC 
GAGCGCCTGA AGGAGCTGTG CCCGGGTAAA GATGCAGATG TGTTGGTGGA GGCGGAGGTG 
CTGCAGGTTC ACGCTCCACC GTTTATAACA AAGGATCGCT CTTGTAACGG CGGCGTAGAC 
CAGGGCGTGG CTCCGGCCTT TAGCCGAGTC CAGAAGAGAC GCTCCTTCAA GAAGAAGAAG 
AGAGAGCGGC CGGTGTCAAC CCTCAGCAAC TCCAGTCAAG CCAGTGAATC TGAAGAGCTG 
AGCGTCCCGC AGAACATTGA TCTGATCGCC CTGCCACAGC TCTGCTTTCC TGATGGGTTG 
AGAATCACCA ATGAGAGCAG AGAAGACTCG TATCACTTCC TGGTGTTCAC GGATGTGTTC 
GGGAATCAGA CTCATGGTGT GGTCGCTCAG TACTGCAGAC CTGTACAGTT CCAGCAAGAT 
AATGTGATTT ATGCAAACGG CCATCGAACC AACGAACACA AGCGTCTGTA CACCACATTC 
AGCATATGTG TGATCTCCAA ATATCCCTAT TATAACGCCC TCAGAGACTG CCTGTCCTGT 
TTTCTGTTCC AGATGAAGAC ATCCCGTGTG TCTGACTTTG ATGAGCAGGT GAAGGAGTTT 
TCTGCCAAAC TGGCGCTGGT GCCCATCCCT CCATCTGGAC CACTGCATGT GGTGTTTAAT 
CTCAGACCGC TTCAGATTGA GCTTCCCTCC AGACTGGATG TGGATCGACC CGTAATGGAC 
CTCGATCTCC ATCTGCCATT TCTGTGCTTT AAACCCAAGC AGATCCTGCA GATCATCAGC 
AGTATCCTGA TGGAGCAGAG AGTGGTGTTT CTGTCCACAG ACTGGGCCAA ACTCACACTG 
GTGTCCGAGT GCTTCATGAT CTTTATTCAC CCTCTGCGCT GGCAGCATCC GTTTGTGCCC 
GTCCTGTCCC GACAGATGCT GGACTTCATC ATGGCGCCCA CGGCCTACCT GATGGGCTGC 
CACACGTACC ACTTCCAGGA GGTGGCAGAG GAGATTGAGG ATCTGGTGCT GATAGACATT 
GACCAGGGAA CAGTGTCGTC TTCAACATCA GACAGATTTG ATCTTCCAGA CGTGCCGCTT 
GCAGCCAGAG ATTGTTTTAT ATTCCGCTGT GGGGGTCTGC AGCTCCACTA TGATCTGGAT 
GTGTGCGGCT CCAGCAGTCA GACTGATGTG AATGATCTGC GAGCTCACAG AAGACTCTGG 
CAGCGCAAAC TCAACTCCAG CATCCTCAAC ATCAGCATGG AGCTCATCGT CAACATATTC 
AGTGAGGTTT GTGATTTCCT GAACTATGAG CATCGAGTTT TCAACAGCGA GGAGTTCCTC 
AGGTCCAGAG ACTCCACCGA CTCTGCCTTT TACAGAAAGG TTTTAGACAC TCATATATTC 
CACTCGTTTC TGCGGGAGCG TTTGAACAGA AAGAATGACG CATTCACTCG CATGCAGCTC 
AACATCCGCT CAGAGACTCG CAGGCTGAAG ATTATGACCG AGTCTCCGCG CAGACCCACG 
ATGGAGGAGA TCAACCGTAA ACACCGAGCA GAGAACGGCC TGAATAAGCG TTTGGGGATG 
AGCATGCCGA ACCTGGCGGA CTCTCGATGT CTCACCGTAC CCATCCGACA GATGTCCATG 
ATGTCAGTGA GGATGCCTTT ACAGATGGGT TCCTGGAGTC CTATAAAGCC AGTCAAAACC 
TTCAAACTCC CAGAATTCCC TCCACCACTG GCCTACCACT ATGTACAGAA CTACTACCAC 
GATCTGATTG GGCAGTTGGA CAGATCCATC TCATCTGCTT CCCCAGACGA CTCTGCTCTC 
TTGGCCCGCT ATTATTACCT GCGTGGACTG GTGAATTCTG TGACTGGACG ACATGTGGAC 
GCCCTCGCAG ACTTCCAGAG CTTATATAAG ACGGACATGG AGATTTTTCC TGCGCAGCTT 
CTGAACACTC TGGTGGAGGC TCTGCCTGTG GAGGAAAAGA GGATTGCGGA GCGCCGCACC 
GATCTGAAGC GTCTCATGAG CAGGGTGAGG AGGGAAAACG AAAAGGACCG AGCGCGGCTG 
GTGGACGGCG GGACGGTGAA GAGGTTTGAG CTGCCCAAAA AACACCTGCA AAAGGAGGAT 
TTTGTGCGGC GCGTTCAGGA GTCGGGAATC GTGAAGGATC TGGAGACCAT CAAGAGGCTG 
TTCGAGGCAC TCACTCTGGG TGTGGAGGAC TCGTGCCGGT CTGTATTTTA CCTTGAGCTG 
GATGGTAAGC GTAGTCTGCA TGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005170034.1
CDS	399..2705
Translation	MADNVPCGLLEACVLLGVSNERLKELCPGKDADVLVEAEVLQVHAPPFITKDRSCNGGVDQGVAPAFSRVQKRRSFKKKKRERPVSTLSNSSQASESEELSVPQNIDLIALPQLCFPDGLRITNESREDSYHFLVFTDVFGNQTHGVVAQYCRPVQFQQDNVIYANGHRTNEHKRLYTTFSICVISKYPYYNALRDCLSCFLFQMKTSRVSDFDEQVKEFSAKLALVPIPPSGPLHVVFNLRPLQIELPSRLDVDRPVMDLDLHLPFLCFKPKQILQIISSILMEQRVVFLSTDWAKLTLVSECFMIFIHPLRWQHPFVPVLSRQMLDFIMAPTAYLMGCHTYHFQEVAEEIEDLVLIDIDQGTVSSSTSDRFDLPDVPLAARDCFIFRCGGLQLHYDLDVCGSSSQTDVNDLRAHRRLWQRKLNSSILNISMELIVNIFSEVCDFLNYEHRVFNSEEFLRSRDSTDSAFYRKVLDTHIFHSFLRERLNRKNDAFTRMQLNIRSETRRLKIMTESPRRPTMEEINRKHRAENGLNKRLGMSMPNLADSRCLTVPIRQMSMMSVRMPLQMGSWSPIKPVKTFKLPEFPPPLAYHYVQNYYHDLIGQLDRSISSASPDDSALLARYYYLRGLVNSVTGRHVDALADFQSLYKTDMEIFPAQLLNTLVEALPVEEKRIAERRTDLKRLMSRVRRENEKDRARLVDGGTVKRFELPKKHLQKEDFVRRVQESGIVKDLETIKRLFEALTLGVEDSCRSVFYLELDGKRSLHV

Target ORF information:

RefSeq Version	XM_005169977.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio DENN/MADD domain containing 3a (dennd3a), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005169977.3

ORF Insert Sequence:

ATGGCTGATA ATGTGCCGTG TGGTCTGCTG GAGGCCTGTG TGCTGCTTGG AGTGTCAAAC 
GAGCGCCTGA AGGAGCTGTG CCCGGGTAAA GATGCAGATG TGTTGGTGGA GGCGGAGGTG 
CTGCAGGTTC ACGCTCCACC GTTTATAACA AAGGATCGCT CTTGTAACGG CGGCGTAGAC 
CAGGGCGTGG CTCCGGCCTT TAGCCGAGTC CAGAAGAGAC GCTCCTTCAA GAAGAAGAAG 
AGAGAGCGGC CGGTGTCAAC CCTCAGCAAC TCCAGTCAAG CCAGTGAATC TGAAGAGCTG 
AGCGTCCCGC AGAACATTGA TCTGATCGCC CTGCCACAGC TCTGCTTTCC TGATGGGTTG 
AGAATCACCA ATGAGAGCAG AGAAGACTCG TATCACTTCC TGGTGTTCAC GGATGTGTTC 
GGGAATCAGA CTCATGGTGT GGTCGCTCAG TACTGCAGAC CTGTACAGTT CCAGCAAGAT 
AATGTGATTT ATGCAAACGG CCATCGAACC AACGAACACA AGCGTCTGTA CACCACATTC 
AGCATATGTG TGATCTCCAA ATATCCCTAT TATAACGCCC TCAGAGACTG CCTGTCCTGT 
TTTCTGTTCC AGATGAAGAC ATCCCGTGTG TCTGACTTTG ATGAGCAGGT GAAGGAGTTT 
TCTGCCAAAC TGGCGCTGGT GCCCATCCCT CCATCTGGAC CACTGCATGT GGTGTTTAAT 
CTCAGACCGC TTCAGATTGA GCTTCCCTCC AGACTGGATG TGGATCGACC CGTAATGGAC 
CTCGATCTCC ATCTGCCATT TCTGTGCTTT AAACCCAAGC AGATCCTGCA GATCATCAGC 
AGTATCCTGA TGGAGCAGAG AGTGGTGTTT CTGTCCACAG ACTGGGCCAA ACTCACACTG 
GTGTCCGAGT GCTTCATGAT CTTTATTCAC CCTCTGCGCT GGCAGCATCC GTTTGTGCCC 
GTCCTGTCCC GACAGATGCT GGACTTCATC ATGGCGCCCA CGGCCTACCT GATGGGCTGC 
CACACGTACC ACTTCCAGGA GGTGGCAGAG GAGATTGAGG ATCTGGTGCT GATAGACATT 
GACCAGGGAA CAGTGTCGTC TTCAACATCA GACAGATTTG ATCTTCCAGA CGTGCCGCTT 
GCAGCCAGAG ATTGTTTTAT ATTCCGCTGT GGGGGTCTGC AGCTCCACTA TGATCTGGAT 
GTGTGCGGCT CCAGCAGTCA GACTGATGTG AATGATCTGC GAGCTCACAG AAGACTCTGG 
CAGCGCAAAC TCAACTCCAG CATCCTCAAC ATCAGCATGG AGCTCATCGT CAACATATTC 
AGTGAGGTTT GTGATTTCCT GAACTATGAG CATCGAGTTT TCAACAGCGA GGAGTTCCTC 
AGGTCCAGAG ACTCCACCGA CTCTGCCTTT TACAGAAAGG TTTTAGACAC TCATATATTC 
CACTCGTTTC TGCGGGAGCG TTTGAACAGA AAGAATGACG CATTCACTCG CATGCAGCTC 
AACATCCGCT CAGAGACTCG CAGGCTGAAG ATTATGACCG AGTCTCCGCG CAGACCCACG 
ATGGAGGAGA TCAACCGTAA ACACCGAGCA GAGAACGGCC TGAATAAGCG TTTGGGGATG 
AGCATGCCGA ACCTGGCGGA CTCTCGATGT CTCACCGTAC CCATCCGACA GATGTCCATG 
ATGTCAGTGA GGATGCCTTT ACAGATGGGT TCCTGGAGTC CTATAAAGCC AGTCAAAACC 
TTCAAACTCC CAGAATTCCC TCCACCACTG GCCTACCACT ATGTACAGAA CTACTACCAC 
GATCTGATTG GGCAGTTGGA CAGATCCATC TCATCTGCTT CCCCAGACGA CTCTGCTCTC 
TTGGCCCGCT ATTATTACCT GCGTGGACTG GTGAATTCTG TGACTGGACG ACATGTGGAC 
GCCCTCGCAG ACTTCCAGAG CTTATATAAG ACGGACATGG AGATTTTTCC TGCGCAGCTT 
CTGAACACTC TGGTGGAGGC TCTGCCTGTG GAGGAAAAGA GGATTGCGGA GCGCCGCACC 
GATCTGAAGC GTCTCATGAG CAGGGTGAGG AGGGAAAACG AAAAGGACCG AGCGCGGCTG 
GTGGACGGCG GGACGGTGAA GAGGTTTGAG CTGCCCAAAA AACACCTGCA AAAGGAGGAT 
TTTGTGCGGC GCGTTCAGGA GTCGGGAATC GTGAAGGATC TGGAGACCAT CAAGAGGCTG 
TTCGAGGCAC TCACTCTGGG TGTGGAGGAC TCGTGCCGGT CTGTATTTTA CCTTGAGCTG 
GATGGTAAGC GTAGTCTGCA TGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DENND3A ( XM_021468228.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_021323903.1 Danio rerio DENN/MADD domain containing 3a (dennd3a), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa150189
Clone ID Related Accession (Same CDS sequence)	XM_021468228.1 , XM_021468227.1
Accession Version	XM_021468228.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3858bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	DENN domain-containing protein 3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007130.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGATA ATGTGCCGTG TGGTCTGCTG GAGGCCTGTG TGCTGCTTGG AGTGTCAAAC 
GAGCGCCTGA AGGAGCTGTG CCCGGGTAAA GATGCAGATG TGTTGGTGGA GGCGGAGGTG 
CTGCAGGTTC ACGCTCCACC GTTTATAACA AAGGATCGCT CTTGTAACGG CGGCGTAGAC 
CAGGGCGTGG CTCCGGCCTT TAGCCGAGTC CAGAAGAGAC GCTCCTTCAA GAAGAAGAAG 
AGAGAGCGGC CGGTGTCAAC CCTCAGCAAC TCCAGTCAAG CCAGTGAATC TGAAGAGCTG 
AGCGTCCCGC AGAACATTGA TCTGATCGCC CTGCCACAGC TCTGCTTTCC TGATGGGTTG 
AGAATCACCA ATGAGAGCAG AGAAGACTCG TATCACTTCC TGGTGTTCAC GGATGTGTTC 
GGGAATCAGA CTCATGGTGT GGTCGCTCAG TACTGCAGAC CTGTACAGTT CCAGCAAGAT 
AATGTGATTT ATGCAAACGG CCATCGAACC AACGAACACA AGCGTCTGTA CACCACATTC 
AGCATATGTG TGATCTCCAA ATATCCCTAT TATAACGCCC TCAGAGACTG CCTGTCCTGT 
TTTCTGTTCC AGATGAAGAC ATCCCGTGTG TCTGACTTTG ATGAGCAGGT GAAGGAGTTT 
TCTGCCAAAC TGGCGCTGGT GCCCATCCCT CCATCTGGAC CACTGCATGT GGTGTTTAAT 
CTCAGACCGC TTCAGATTGA GCTTCCCTCC AGACTGGATG TGGATCGACC CGTAATGGAC 
CTCGATCTCC ATCTGCCATT TCTGTGCTTT AAACCCAAGC AGATCCTGCA GATCATCAGC 
AGTATCCTGA TGGAGCAGAG AGTGGTGTTT CTGTCCACAG ACTGGGCCAA ACTCACACTG 
GTGTCCGAGT GCTTCATGAT CTTTATTCAC CCTCTGCGCT GGCAGCATCC GTTTGTGCCC 
GTCCTGTCCC GACAGATGCT GGACTTCATC ATGGCGCCCA CGGCCTACCT GATGGGCTGC 
CACACGTACC ACTTCCAGGA GGTGGCAGAG GAGATTGAGG ATCTGGTGCT GATAGACATT 
GACCAGGGAA CAGTGTCGTC TTCAACATCA GACAGATTTG ATCTTCCAGA CGTGCCGCTT 
GCAGCCAGAG ATTGTTTTAT ATTCCGCTGT GGGGGTCTGC AGCTCCACTA TGATCTGGAT 
GTGTGCGGCT CCAGCAGTCA GACTGATGTG AATGATCTGC GAGCTCACAG AAGACTCTGG 
CAGCGCAAAC TCAACTCCAG CATCCTCAAC ATCAGCATGG AGCTCATCGT CAACATATTC 
AGTGAGGTTT GTGATTTCCT GAACTATGAG CATCGAGTTT TCAACAGCGA GGAGTTCCTC 
AGGTCCAGAG ACTCCACCGA CTCTGCCTTT TACAGAAAGG TTTTAGACAC TCATATATTC 
CACTCGTTTC TGCGGGAGCG TTTGAACAGA AAGAATGACG CATTCACTCG CATGCAGCTC 
AACATCCGCT CAGAGACTCG CAGGCTGAAG ATTATGACCG AGTCTCCGCG CAGACCCACG 
ATGGAGGAGA TCAACCGTAA ACACCGAGCA GAGAACGGCC TGAATAAGCG TTTGGGGATG 
AGCATGCCGA ACCTGGCGGA CTCTCGATCT CTCACCGTAC CCATCCGACA GATGTCCATG 
ATGTCAGTGA GGATGCCTTT ACAGATGGGT TCCTGGAGTC CTATAAAGCC AGTCAAAACC 
TTCAAACTCC CAGAATTCCC TCCACCACTG GCCTACCACT ATGTACAGAA CTACTACCAC 
GATCTGATTG GGCAGTTGGA CAGATCCATC TCATCTGCTT CCCCAGACGA CTCTGCTCTC 
TTGGCCCGCT ATTATTACCT GCGTGGACTG GTGAATTCTG TGACTGGACG ACATGTGGAC 
GCCCTCGCAG ACTTCCAGAG CTTATATAAG ACGGACATGG AGATTTTTCC TGCGCAGCTT 
CTGAACACTC TGGTGGAGGC TCTGCCTGTG GAGGAAAAGA GGATTGCGGA GCGCCGCACC 
GATCTGAAGC GTCTCATGAG CAGGGTGAGG AGGGAAAACG AAAAGGACCG AGCGCGGCTG 
GTGGACGGCG GGACGGTGAA GAGGTTTGAG CTGCCCAAAA AACACCTGCA AAAGGAGGAT 
TTTGTGCGGC GCGTTCAGGA GTCGGGAATC GTGAAGGATC TGGAGACCAT CAAGAGGCTG 
TTCGAGGCAC TCACTCTGGG TGTGGAGGAC TCGTGCCGGT CTGTATTTTA CCTTGAGCTG 
GATGGTCAGG TGAAGCAGGT GGATCCAGAA ACCTTCCGCT TCTTCTACAA CTTCTGGAAG 
GAAACGGAAG CGGCGGCGCA GGACGTGGAT CTGCCGGCCG TCGTGTTGGA GCATCTGGAG 
AACGATGAGT GTGTTTACAA AGTCTCGTCA TCTGTAAAAA CCAGCCACGG TGTGGGGAAG 
ATCGCCATGA CCCAGCGCAG ACTCTTCCTG CTGACGGAGG GACGACCCGG ATACGTGGAG 
ATCACCAAAT TCAGAGACAT CGAGGATGTA AGGATTTCCT CTGCCCCGTT CCTCCTCCTG 
AGAATCCCAT CACTGAAGAT CAGGACGTCC CTCAGGAGGG AGTCATTCGA GGCCAATCTG 
AAGTCCGAGT GTGACCTCTG GCACCTGATG ATCAAAGAGA TGTGGGCCGG GAGGAAGATG 
GCGGACCAAC ATAAGGATCC TCAGTTTATG GAGCAGGCTT TGACTAACGC TCTTCTGATG 
GACGCTGTGG TGGGCAGTCT GCAGTCTCAG AAAGCCATCT ACGCTGCCAC TAAACTGGCT 
TACTTTGACA AGATGAAGTC TGAGGTGCCC ATGATGGTCC CCAAAACCAC CTCTGACACC 
CTGAAGCACA AGATCAACCC GTCTCTGGAC CAGACGAGTC CTCAAGCAGT GGACGTTCTG 
CTCTACACTC CAGGTCAGCT GGGTGTGTCA GATGTGGACG GTGGTGGGAA CCCGAAGCTG 
TGGTGTGCTC TGAGTGATGG GAAGCTGCTA GTTTTTGACG CCGCCAGCTG GTCCATGCAG 
CACAACTCTG TTCAAGTCGG GACGTCCCGT CTGACGTGTA TGCTGGCAGT GAATGAGCAG 
CAGCTGTGGA TCGGCTCTAA AGATTCCTTC ATCTACATCA TAAACCCGCG CAGCGTCTCC 
TGCAATAAAC AGCTGACGGA GCATCGCTGT GAGCTCACCG GGCTCGCCCT GGAGGAGAGA 
GCCGACAAAT TCAGCCAGAC GGTGGCGTAC TCCTGCAGTG AGGACTGCAA TGTGATCGTG 
TGGGACGTTT CCTCACTGCA GGTCAGACGG CAGTTCAGAG TGTCCTGCGG CCGACTGCAG 
TCCATGCACG TCCACAACGG CATGCTGTGG TGCTGTGGTC GTGATTGTGT GCTGGAGGTG 
CGCAGGAACG GCTCGGTTCA CCGTCGGATC TCTCTGCCCG ATCACCTGCT GAGCTCACCA 
AAGGGCTTCA GCAGTTTCCT GCTCTTTAAT GAGTCGGAGC AGCTGTGGGC TGGTTTTGCG 
GATGCGGGTG AGCTGTGTGT TTGGCACTGC AGAGACATCA GCAAACCCTT CATCAGGATT 
CAGCTCAAAG ACTGCGCCGC CGTCACCTGC ATGATCAGGG TCAAGAACCA GATCTGGGTC 
GGCGGTTACA ATGGCAGCGT CACAGACAGA CGAGTGCGAG GGAAGATCTA CATAGTAAAC 
ACGGACCGAT ATACAGTGGA GAAAGAGCTG CAGGCCCACG CCGACGCCGT TCAGACCATG 
TGTTCAGCCG AAGACCGATA TGTGCTCAGC GGAGCCGCAC AGGATGATGG GAAAATCGGC 
ATCTGGAAGG TGGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021323903.1
CDS	401..4258
Translation	MADNVPCGLLEACVLLGVSNERLKELCPGKDADVLVEAEVLQVHAPPFITKDRSCNGGVDQGVAPAFSRVQKRRSFKKKKRERPVSTLSNSSQASESEELSVPQNIDLIALPQLCFPDGLRITNESREDSYHFLVFTDVFGNQTHGVVAQYCRPVQFQQDNVIYANGHRTNEHKRLYTTFSICVISKYPYYNALRDCLSCFLFQMKTSRVSDFDEQVKEFSAKLALVPIPPSGPLHVVFNLRPLQIELPSRLDVDRPVMDLDLHLPFLCFKPKQILQIISSILMEQRVVFLSTDWAKLTLVSECFMIFIHPLRWQHPFVPVLSRQMLDFIMAPTAYLMGCHTYHFQEVAEEIEDLVLIDIDQGTVSSSTSDRFDLPDVPLAARDCFIFRCGGLQLHYDLDVCGSSSQTDVNDLRAHRRLWQRKLNSSILNISMELIVNIFSEVCDFLNYEHRVFNSEEFLRSRDSTDSAFYRKVLDTHIFHSFLRERLNRKNDAFTRMQLNIRSETRRLKIMTESPRRPTMEEINRKHRAENGLNKRLGMSMPNLADSRSLTVPIRQMSMMSVRMPLQMGSWSPIKPVKTFKLPEFPPPLAYHYVQNYYHDLIGQLDRSISSASPDDSALLARYYYLRGLVNSVTGRHVDALADFQSLYKTDMEIFPAQLLNTLVEALPVEEKRIAERRTDLKRLMSRVRRENEKDRARLVDGGTVKRFELPKKHLQKEDFVRRVQESGIVKDLETIKRLFEALTLGVEDSCRSVFYLELDGQVKQVDPETFRFFYNFWKETEAAAQDVDLPAVVLEHLENDECVYKVSSSVKTSHGVGKIAMTQRRLFLLTEGRPGYVEITKFRDIEDVRISSAPFLLLRIPSLKIRTSLRRESFEANLKSECDLWHLMIKEMWAGRKMADQHKDPQFMEQALTNALLMDAVVGSLQSQKAIYAATKLAYFDKMKSEVPMMVPKTTSDTLKHKINPSLDQTSPQAVDVLLYTPGQLGVSDVDGGGNPKLWCALSDGKLLVFDAASWSMQHNSVQVGTSRLTCMLAVNEQQLWIGSKDSFIYIINPRSVSCNKQLTEHRCELTGLALEERADKFSQTVAYSCSEDCNVIVWDVSSLQVRRQFRVSCGRLQSMHVHNGMLWCCGRDCVLEVRRNGSVHRRISLPDHLLSSPKGFSSFLLFNESEQLWAGFADAGELCVWHCRDISKPFIRIQLKDCAAVTCMIRVKNQIWVGGYNGSVTDRRVRGKIYIVNTDRYTVEKELQAHADAVQTMCSAEDRYVLSGAAQDDGKIGIWKVE

Target ORF information:

RefSeq Version	XM_021468228.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio DENN/MADD domain containing 3a (dennd3a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021468228.1

ORF Insert Sequence:

ATGGCTGATA ATGTGCCGTG TGGTCTGCTG GAGGCCTGTG TGCTGCTTGG AGTGTCAAAC 
GAGCGCCTGA AGGAGCTGTG CCCGGGTAAA GATGCAGATG TGTTGGTGGA GGCGGAGGTG 
CTGCAGGTTC ACGCTCCACC GTTTATAACA AAGGATCGCT CTTGTAACGG CGGCGTAGAC 
CAGGGCGTGG CTCCGGCCTT TAGCCGAGTC CAGAAGAGAC GCTCCTTCAA GAAGAAGAAG 
AGAGAGCGGC CGGTGTCAAC CCTCAGCAAC TCCAGTCAAG CCAGTGAATC TGAAGAGCTG 
AGCGTCCCGC AGAACATTGA TCTGATCGCC CTGCCACAGC TCTGCTTTCC TGATGGGTTG 
AGAATCACCA ATGAGAGCAG AGAAGACTCG TATCACTTCC TGGTGTTCAC GGATGTGTTC 
GGGAATCAGA CTCATGGTGT GGTCGCTCAG TACTGCAGAC CTGTACAGTT CCAGCAAGAT 
AATGTGATTT ATGCAAACGG CCATCGAACC AACGAACACA AGCGTCTGTA CACCACATTC 
AGCATATGTG TGATCTCCAA ATATCCCTAT TATAACGCCC TCAGAGACTG CCTGTCCTGT 
TTTCTGTTCC AGATGAAGAC ATCCCGTGTG TCTGACTTTG ATGAGCAGGT GAAGGAGTTT 
TCTGCCAAAC TGGCGCTGGT GCCCATCCCT CCATCTGGAC CACTGCATGT GGTGTTTAAT 
CTCAGACCGC TTCAGATTGA GCTTCCCTCC AGACTGGATG TGGATCGACC CGTAATGGAC 
CTCGATCTCC ATCTGCCATT TCTGTGCTTT AAACCCAAGC AGATCCTGCA GATCATCAGC 
AGTATCCTGA TGGAGCAGAG AGTGGTGTTT CTGTCCACAG ACTGGGCCAA ACTCACACTG 
GTGTCCGAGT GCTTCATGAT CTTTATTCAC CCTCTGCGCT GGCAGCATCC GTTTGTGCCC 
GTCCTGTCCC GACAGATGCT GGACTTCATC ATGGCGCCCA CGGCCTACCT GATGGGCTGC 
CACACGTACC ACTTCCAGGA GGTGGCAGAG GAGATTGAGG ATCTGGTGCT GATAGACATT 
GACCAGGGAA CAGTGTCGTC TTCAACATCA GACAGATTTG ATCTTCCAGA CGTGCCGCTT 
GCAGCCAGAG ATTGTTTTAT ATTCCGCTGT GGGGGTCTGC AGCTCCACTA TGATCTGGAT 
GTGTGCGGCT CCAGCAGTCA GACTGATGTG AATGATCTGC GAGCTCACAG AAGACTCTGG 
CAGCGCAAAC TCAACTCCAG CATCCTCAAC ATCAGCATGG AGCTCATCGT CAACATATTC 
AGTGAGGTTT GTGATTTCCT GAACTATGAG CATCGAGTTT TCAACAGCGA GGAGTTCCTC 
AGGTCCAGAG ACTCCACCGA CTCTGCCTTT TACAGAAAGG TTTTAGACAC TCATATATTC 
CACTCGTTTC TGCGGGAGCG TTTGAACAGA AAGAATGACG CATTCACTCG CATGCAGCTC 
AACATCCGCT CAGAGACTCG CAGGCTGAAG ATTATGACCG AGTCTCCGCG CAGACCCACG 
ATGGAGGAGA TCAACCGTAA ACACCGAGCA GAGAACGGCC TGAATAAGCG TTTGGGGATG 
AGCATGCCGA ACCTGGCGGA CTCTCGATCT CTCACCGTAC CCATCCGACA GATGTCCATG 
ATGTCAGTGA GGATGCCTTT ACAGATGGGT TCCTGGAGTC CTATAAAGCC AGTCAAAACC 
TTCAAACTCC CAGAATTCCC TCCACCACTG GCCTACCACT ATGTACAGAA CTACTACCAC 
GATCTGATTG GGCAGTTGGA CAGATCCATC TCATCTGCTT CCCCAGACGA CTCTGCTCTC 
TTGGCCCGCT ATTATTACCT GCGTGGACTG GTGAATTCTG TGACTGGACG ACATGTGGAC 
GCCCTCGCAG ACTTCCAGAG CTTATATAAG ACGGACATGG AGATTTTTCC TGCGCAGCTT 
CTGAACACTC TGGTGGAGGC TCTGCCTGTG GAGGAAAAGA GGATTGCGGA GCGCCGCACC 
GATCTGAAGC GTCTCATGAG CAGGGTGAGG AGGGAAAACG AAAAGGACCG AGCGCGGCTG 
GTGGACGGCG GGACGGTGAA GAGGTTTGAG CTGCCCAAAA AACACCTGCA AAAGGAGGAT 
TTTGTGCGGC GCGTTCAGGA GTCGGGAATC GTGAAGGATC TGGAGACCAT CAAGAGGCTG 
TTCGAGGCAC TCACTCTGGG TGTGGAGGAC TCGTGCCGGT CTGTATTTTA CCTTGAGCTG 
GATGGTCAGG TGAAGCAGGT GGATCCAGAA ACCTTCCGCT TCTTCTACAA CTTCTGGAAG 
GAAACGGAAG CGGCGGCGCA GGACGTGGAT CTGCCGGCCG TCGTGTTGGA GCATCTGGAG 
AACGATGAGT GTGTTTACAA AGTCTCGTCA TCTGTAAAAA CCAGCCACGG TGTGGGGAAG 
ATCGCCATGA CCCAGCGCAG ACTCTTCCTG CTGACGGAGG GACGACCCGG ATACGTGGAG 
ATCACCAAAT TCAGAGACAT CGAGGATGTA AGGATTTCCT CTGCCCCGTT CCTCCTCCTG 
AGAATCCCAT CACTGAAGAT CAGGACGTCC CTCAGGAGGG AGTCATTCGA GGCCAATCTG 
AAGTCCGAGT GTGACCTCTG GCACCTGATG ATCAAAGAGA TGTGGGCCGG GAGGAAGATG 
GCGGACCAAC ATAAGGATCC TCAGTTTATG GAGCAGGCTT TGACTAACGC TCTTCTGATG 
GACGCTGTGG TGGGCAGTCT GCAGTCTCAG AAAGCCATCT ACGCTGCCAC TAAACTGGCT 
TACTTTGACA AGATGAAGTC TGAGGTGCCC ATGATGGTCC CCAAAACCAC CTCTGACACC 
CTGAAGCACA AGATCAACCC GTCTCTGGAC CAGACGAGTC CTCAAGCAGT GGACGTTCTG 
CTCTACACTC CAGGTCAGCT GGGTGTGTCA GATGTGGACG GTGGTGGGAA CCCGAAGCTG 
TGGTGTGCTC TGAGTGATGG GAAGCTGCTA GTTTTTGACG CCGCCAGCTG GTCCATGCAG 
CACAACTCTG TTCAAGTCGG GACGTCCCGT CTGACGTGTA TGCTGGCAGT GAATGAGCAG 
CAGCTGTGGA TCGGCTCTAA AGATTCCTTC ATCTACATCA TAAACCCGCG CAGCGTCTCC 
TGCAATAAAC AGCTGACGGA GCATCGCTGT GAGCTCACCG GGCTCGCCCT GGAGGAGAGA 
GCCGACAAAT TCAGCCAGAC GGTGGCGTAC TCCTGCAGTG AGGACTGCAA TGTGATCGTG 
TGGGACGTTT CCTCACTGCA GGTCAGACGG CAGTTCAGAG TGTCCTGCGG CCGACTGCAG 
TCCATGCACG TCCACAACGG CATGCTGTGG TGCTGTGGTC GTGATTGTGT GCTGGAGGTG 
CGCAGGAACG GCTCGGTTCA CCGTCGGATC TCTCTGCCCG ATCACCTGCT GAGCTCACCA 
AAGGGCTTCA GCAGTTTCCT GCTCTTTAAT GAGTCGGAGC AGCTGTGGGC TGGTTTTGCG 
GATGCGGGTG AGCTGTGTGT TTGGCACTGC AGAGACATCA GCAAACCCTT CATCAGGATT 
CAGCTCAAAG ACTGCGCCGC CGTCACCTGC ATGATCAGGG TCAAGAACCA GATCTGGGTC 
GGCGGTTACA ATGGCAGCGT CACAGACAGA CGAGTGCGAG GGAAGATCTA CATAGTAAAC 
ACGGACCGAT ATACAGTGGA GAAAGAGCTG CAGGCCCACG CCGACGCCGT TCAGACCATG 
TGTTCAGCCG AAGACCGATA TGTGCTCAGC GGAGCCGCAC AGGATGATGG GAAAATCGGC 
ATCTGGAAGG TGGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DENND3A ( XM_021468227.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_021323902.1 Danio rerio DENN/MADD domain containing 3a (dennd3a), transcript variant X1, mRNA.

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CloneID	ODa150189
Clone ID Related Accession (Same CDS sequence)	XM_021468228.1 , XM_021468227.1
Accession Version	XM_021468227.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3858bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	DENN domain-containing protein 3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007130.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGATA ATGTGCCGTG TGGTCTGCTG GAGGCCTGTG TGCTGCTTGG AGTGTCAAAC 
GAGCGCCTGA AGGAGCTGTG CCCGGGTAAA GATGCAGATG TGTTGGTGGA GGCGGAGGTG 
CTGCAGGTTC ACGCTCCACC GTTTATAACA AAGGATCGCT CTTGTAACGG CGGCGTAGAC 
CAGGGCGTGG CTCCGGCCTT TAGCCGAGTC CAGAAGAGAC GCTCCTTCAA GAAGAAGAAG 
AGAGAGCGGC CGGTGTCAAC CCTCAGCAAC TCCAGTCAAG CCAGTGAATC TGAAGAGCTG 
AGCGTCCCGC AGAACATTGA TCTGATCGCC CTGCCACAGC TCTGCTTTCC TGATGGGTTG 
AGAATCACCA ATGAGAGCAG AGAAGACTCG TATCACTTCC TGGTGTTCAC GGATGTGTTC 
GGGAATCAGA CTCATGGTGT GGTCGCTCAG TACTGCAGAC CTGTACAGTT CCAGCAAGAT 
AATGTGATTT ATGCAAACGG CCATCGAACC AACGAACACA AGCGTCTGTA CACCACATTC 
AGCATATGTG TGATCTCCAA ATATCCCTAT TATAACGCCC TCAGAGACTG CCTGTCCTGT 
TTTCTGTTCC AGATGAAGAC ATCCCGTGTG TCTGACTTTG ATGAGCAGGT GAAGGAGTTT 
TCTGCCAAAC TGGCGCTGGT GCCCATCCCT CCATCTGGAC CACTGCATGT GGTGTTTAAT 
CTCAGACCGC TTCAGATTGA GCTTCCCTCC AGACTGGATG TGGATCGACC CGTAATGGAC 
CTCGATCTCC ATCTGCCATT TCTGTGCTTT AAACCCAAGC AGATCCTGCA GATCATCAGC 
AGTATCCTGA TGGAGCAGAG AGTGGTGTTT CTGTCCACAG ACTGGGCCAA ACTCACACTG 
GTGTCCGAGT GCTTCATGAT CTTTATTCAC CCTCTGCGCT GGCAGCATCC GTTTGTGCCC 
GTCCTGTCCC GACAGATGCT GGACTTCATC ATGGCGCCCA CGGCCTACCT GATGGGCTGC 
CACACGTACC ACTTCCAGGA GGTGGCAGAG GAGATTGAGG ATCTGGTGCT GATAGACATT 
GACCAGGGAA CAGTGTCGTC TTCAACATCA GACAGATTTG ATCTTCCAGA CGTGCCGCTT 
GCAGCCAGAG ATTGTTTTAT ATTCCGCTGT GGGGGTCTGC AGCTCCACTA TGATCTGGAT 
GTGTGCGGCT CCAGCAGTCA GACTGATGTG AATGATCTGC GAGCTCACAG AAGACTCTGG 
CAGCGCAAAC TCAACTCCAG CATCCTCAAC ATCAGCATGG AGCTCATCGT CAACATATTC 
AGTGAGGTTT GTGATTTCCT GAACTATGAG CATCGAGTTT TCAACAGCGA GGAGTTCCTC 
AGGTCCAGAG ACTCCACCGA CTCTGCCTTT TACAGAAAGG TTTTAGACAC TCATATATTC 
CACTCGTTTC TGCGGGAGCG TTTGAACAGA AAGAATGACG CATTCACTCG CATGCAGCTC 
AACATCCGCT CAGAGACTCG CAGGCTGAAG ATTATGACCG AGTCTCCGCG CAGACCCACG 
ATGGAGGAGA TCAACCGTAA ACACCGAGCA GAGAACGGCC TGAATAAGCG TTTGGGGATG 
AGCATGCCGA ACCTGGCGGA CTCTCGATCT CTCACCGTAC CCATCCGACA GATGTCCATG 
ATGTCAGTGA GGATGCCTTT ACAGATGGGT TCCTGGAGTC CTATAAAGCC AGTCAAAACC 
TTCAAACTCC CAGAATTCCC TCCACCACTG GCCTACCACT ATGTACAGAA CTACTACCAC 
GATCTGATTG GGCAGTTGGA CAGATCCATC TCATCTGCTT CCCCAGACGA CTCTGCTCTC 
TTGGCCCGCT ATTATTACCT GCGTGGACTG GTGAATTCTG TGACTGGACG ACATGTGGAC 
GCCCTCGCAG ACTTCCAGAG CTTATATAAG ACGGACATGG AGATTTTTCC TGCGCAGCTT 
CTGAACACTC TGGTGGAGGC TCTGCCTGTG GAGGAAAAGA GGATTGCGGA GCGCCGCACC 
GATCTGAAGC GTCTCATGAG CAGGGTGAGG AGGGAAAACG AAAAGGACCG AGCGCGGCTG 
GTGGACGGCG GGACGGTGAA GAGGTTTGAG CTGCCCAAAA AACACCTGCA AAAGGAGGAT 
TTTGTGCGGC GCGTTCAGGA GTCGGGAATC GTGAAGGATC TGGAGACCAT CAAGAGGCTG 
TTCGAGGCAC TCACTCTGGG TGTGGAGGAC TCGTGCCGGT CTGTATTTTA CCTTGAGCTG 
GATGGTCAGG TGAAGCAGGT GGATCCAGAA ACCTTCCGCT TCTTCTACAA CTTCTGGAAG 
GAAACGGAAG CGGCGGCGCA GGACGTGGAT CTGCCGGCCG TCGTGTTGGA GCATCTGGAG 
AACGATGAGT GTGTTTACAA AGTCTCGTCA TCTGTAAAAA CCAGCCACGG TGTGGGGAAG 
ATCGCCATGA CCCAGCGCAG ACTCTTCCTG CTGACGGAGG GACGACCCGG ATACGTGGAG 
ATCACCAAAT TCAGAGACAT CGAGGATGTA AGGATTTCCT CTGCCCCGTT CCTCCTCCTG 
AGAATCCCAT CACTGAAGAT CAGGACGTCC CTCAGGAGGG AGTCATTCGA GGCCAATCTG 
AAGTCCGAGT GTGACCTCTG GCACCTGATG ATCAAAGAGA TGTGGGCCGG GAGGAAGATG 
GCGGACCAAC ATAAGGATCC TCAGTTTATG GAGCAGGCTT TGACTAACGC TCTTCTGATG 
GACGCTGTGG TGGGCAGTCT GCAGTCTCAG AAAGCCATCT ACGCTGCCAC TAAACTGGCT 
TACTTTGACA AGATGAAGTC TGAGGTGCCC ATGATGGTCC CCAAAACCAC CTCTGACACC 
CTGAAGCACA AGATCAACCC GTCTCTGGAC CAGACGAGTC CTCAAGCAGT GGACGTTCTG 
CTCTACACTC CAGGTCAGCT GGGTGTGTCA GATGTGGACG GTGGTGGGAA CCCGAAGCTG 
TGGTGTGCTC TGAGTGATGG GAAGCTGCTA GTTTTTGACG CCGCCAGCTG GTCCATGCAG 
CACAACTCTG TTCAAGTCGG GACGTCCCGT CTGACGTGTA TGCTGGCAGT GAATGAGCAG 
CAGCTGTGGA TCGGCTCTAA AGATTCCTTC ATCTACATCA TAAACCCGCG CAGCGTCTCC 
TGCAATAAAC AGCTGACGGA GCATCGCTGT GAGCTCACCG GGCTCGCCCT GGAGGAGAGA 
GCCGACAAAT TCAGCCAGAC GGTGGCGTAC TCCTGCAGTG AGGACTGCAA TGTGATCGTG 
TGGGACGTTT CCTCACTGCA GGTCAGACGG CAGTTCAGAG TGTCCTGCGG CCGACTGCAG 
TCCATGCACG TCCACAACGG CATGCTGTGG TGCTGTGGTC GTGATTGTGT GCTGGAGGTG 
CGCAGGAACG GCTCGGTTCA CCGTCGGATC TCTCTGCCCG ATCACCTGCT GAGCTCACCA 
AAGGGCTTCA GCAGTTTCCT GCTCTTTAAT GAGTCGGAGC AGCTGTGGGC TGGTTTTGCG 
GATGCGGGTG AGCTGTGTGT TTGGCACTGC AGAGACATCA GCAAACCCTT CATCAGGATT 
CAGCTCAAAG ACTGCGCCGC CGTCACCTGC ATGATCAGGG TCAAGAACCA GATCTGGGTC 
GGCGGTTACA ATGGCAGCGT CACAGACAGA CGAGTGCGAG GGAAGATCTA CATAGTAAAC 
ACGGACCGAT ATACAGTGGA GAAAGAGCTG CAGGCCCACG CCGACGCCGT TCAGACCATG 
TGTTCAGCCG AAGACCGATA TGTGCTCAGC GGAGCCGCAC AGGATGATGG GAAAATCGGC 
ATCTGGAAGG TGGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021323902.1
CDS	397..4254
Translation	MADNVPCGLLEACVLLGVSNERLKELCPGKDADVLVEAEVLQVHAPPFITKDRSCNGGVDQGVAPAFSRVQKRRSFKKKKRERPVSTLSNSSQASESEELSVPQNIDLIALPQLCFPDGLRITNESREDSYHFLVFTDVFGNQTHGVVAQYCRPVQFQQDNVIYANGHRTNEHKRLYTTFSICVISKYPYYNALRDCLSCFLFQMKTSRVSDFDEQVKEFSAKLALVPIPPSGPLHVVFNLRPLQIELPSRLDVDRPVMDLDLHLPFLCFKPKQILQIISSILMEQRVVFLSTDWAKLTLVSECFMIFIHPLRWQHPFVPVLSRQMLDFIMAPTAYLMGCHTYHFQEVAEEIEDLVLIDIDQGTVSSSTSDRFDLPDVPLAARDCFIFRCGGLQLHYDLDVCGSSSQTDVNDLRAHRRLWQRKLNSSILNISMELIVNIFSEVCDFLNYEHRVFNSEEFLRSRDSTDSAFYRKVLDTHIFHSFLRERLNRKNDAFTRMQLNIRSETRRLKIMTESPRRPTMEEINRKHRAENGLNKRLGMSMPNLADSRSLTVPIRQMSMMSVRMPLQMGSWSPIKPVKTFKLPEFPPPLAYHYVQNYYHDLIGQLDRSISSASPDDSALLARYYYLRGLVNSVTGRHVDALADFQSLYKTDMEIFPAQLLNTLVEALPVEEKRIAERRTDLKRLMSRVRRENEKDRARLVDGGTVKRFELPKKHLQKEDFVRRVQESGIVKDLETIKRLFEALTLGVEDSCRSVFYLELDGQVKQVDPETFRFFYNFWKETEAAAQDVDLPAVVLEHLENDECVYKVSSSVKTSHGVGKIAMTQRRLFLLTEGRPGYVEITKFRDIEDVRISSAPFLLLRIPSLKIRTSLRRESFEANLKSECDLWHLMIKEMWAGRKMADQHKDPQFMEQALTNALLMDAVVGSLQSQKAIYAATKLAYFDKMKSEVPMMVPKTTSDTLKHKINPSLDQTSPQAVDVLLYTPGQLGVSDVDGGGNPKLWCALSDGKLLVFDAASWSMQHNSVQVGTSRLTCMLAVNEQQLWIGSKDSFIYIINPRSVSCNKQLTEHRCELTGLALEERADKFSQTVAYSCSEDCNVIVWDVSSLQVRRQFRVSCGRLQSMHVHNGMLWCCGRDCVLEVRRNGSVHRRISLPDHLLSSPKGFSSFLLFNESEQLWAGFADAGELCVWHCRDISKPFIRIQLKDCAAVTCMIRVKNQIWVGGYNGSVTDRRVRGKIYIVNTDRYTVEKELQAHADAVQTMCSAEDRYVLSGAAQDDGKIGIWKVE

Target ORF information:

RefSeq Version	XM_021468227.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio DENN/MADD domain containing 3a (dennd3a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021468227.1

ORF Insert Sequence:

ATGGCTGATA ATGTGCCGTG TGGTCTGCTG GAGGCCTGTG TGCTGCTTGG AGTGTCAAAC 
GAGCGCCTGA AGGAGCTGTG CCCGGGTAAA GATGCAGATG TGTTGGTGGA GGCGGAGGTG 
CTGCAGGTTC ACGCTCCACC GTTTATAACA AAGGATCGCT CTTGTAACGG CGGCGTAGAC 
CAGGGCGTGG CTCCGGCCTT TAGCCGAGTC CAGAAGAGAC GCTCCTTCAA GAAGAAGAAG 
AGAGAGCGGC CGGTGTCAAC CCTCAGCAAC TCCAGTCAAG CCAGTGAATC TGAAGAGCTG 
AGCGTCCCGC AGAACATTGA TCTGATCGCC CTGCCACAGC TCTGCTTTCC TGATGGGTTG 
AGAATCACCA ATGAGAGCAG AGAAGACTCG TATCACTTCC TGGTGTTCAC GGATGTGTTC 
GGGAATCAGA CTCATGGTGT GGTCGCTCAG TACTGCAGAC CTGTACAGTT CCAGCAAGAT 
AATGTGATTT ATGCAAACGG CCATCGAACC AACGAACACA AGCGTCTGTA CACCACATTC 
AGCATATGTG TGATCTCCAA ATATCCCTAT TATAACGCCC TCAGAGACTG CCTGTCCTGT 
TTTCTGTTCC AGATGAAGAC ATCCCGTGTG TCTGACTTTG ATGAGCAGGT GAAGGAGTTT 
TCTGCCAAAC TGGCGCTGGT GCCCATCCCT CCATCTGGAC CACTGCATGT GGTGTTTAAT 
CTCAGACCGC TTCAGATTGA GCTTCCCTCC AGACTGGATG TGGATCGACC CGTAATGGAC 
CTCGATCTCC ATCTGCCATT TCTGTGCTTT AAACCCAAGC AGATCCTGCA GATCATCAGC 
AGTATCCTGA TGGAGCAGAG AGTGGTGTTT CTGTCCACAG ACTGGGCCAA ACTCACACTG 
GTGTCCGAGT GCTTCATGAT CTTTATTCAC CCTCTGCGCT GGCAGCATCC GTTTGTGCCC 
GTCCTGTCCC GACAGATGCT GGACTTCATC ATGGCGCCCA CGGCCTACCT GATGGGCTGC 
CACACGTACC ACTTCCAGGA GGTGGCAGAG GAGATTGAGG ATCTGGTGCT GATAGACATT 
GACCAGGGAA CAGTGTCGTC TTCAACATCA GACAGATTTG ATCTTCCAGA CGTGCCGCTT 
GCAGCCAGAG ATTGTTTTAT ATTCCGCTGT GGGGGTCTGC AGCTCCACTA TGATCTGGAT 
GTGTGCGGCT CCAGCAGTCA GACTGATGTG AATGATCTGC GAGCTCACAG AAGACTCTGG 
CAGCGCAAAC TCAACTCCAG CATCCTCAAC ATCAGCATGG AGCTCATCGT CAACATATTC 
AGTGAGGTTT GTGATTTCCT GAACTATGAG CATCGAGTTT TCAACAGCGA GGAGTTCCTC 
AGGTCCAGAG ACTCCACCGA CTCTGCCTTT TACAGAAAGG TTTTAGACAC TCATATATTC 
CACTCGTTTC TGCGGGAGCG TTTGAACAGA AAGAATGACG CATTCACTCG CATGCAGCTC 
AACATCCGCT CAGAGACTCG CAGGCTGAAG ATTATGACCG AGTCTCCGCG CAGACCCACG 
ATGGAGGAGA TCAACCGTAA ACACCGAGCA GAGAACGGCC TGAATAAGCG TTTGGGGATG 
AGCATGCCGA ACCTGGCGGA CTCTCGATCT CTCACCGTAC CCATCCGACA GATGTCCATG 
ATGTCAGTGA GGATGCCTTT ACAGATGGGT TCCTGGAGTC CTATAAAGCC AGTCAAAACC 
TTCAAACTCC CAGAATTCCC TCCACCACTG GCCTACCACT ATGTACAGAA CTACTACCAC 
GATCTGATTG GGCAGTTGGA CAGATCCATC TCATCTGCTT CCCCAGACGA CTCTGCTCTC 
TTGGCCCGCT ATTATTACCT GCGTGGACTG GTGAATTCTG TGACTGGACG ACATGTGGAC 
GCCCTCGCAG ACTTCCAGAG CTTATATAAG ACGGACATGG AGATTTTTCC TGCGCAGCTT 
CTGAACACTC TGGTGGAGGC TCTGCCTGTG GAGGAAAAGA GGATTGCGGA GCGCCGCACC 
GATCTGAAGC GTCTCATGAG CAGGGTGAGG AGGGAAAACG AAAAGGACCG AGCGCGGCTG 
GTGGACGGCG GGACGGTGAA GAGGTTTGAG CTGCCCAAAA AACACCTGCA AAAGGAGGAT 
TTTGTGCGGC GCGTTCAGGA GTCGGGAATC GTGAAGGATC TGGAGACCAT CAAGAGGCTG 
TTCGAGGCAC TCACTCTGGG TGTGGAGGAC TCGTGCCGGT CTGTATTTTA CCTTGAGCTG 
GATGGTCAGG TGAAGCAGGT GGATCCAGAA ACCTTCCGCT TCTTCTACAA CTTCTGGAAG 
GAAACGGAAG CGGCGGCGCA GGACGTGGAT CTGCCGGCCG TCGTGTTGGA GCATCTGGAG 
AACGATGAGT GTGTTTACAA AGTCTCGTCA TCTGTAAAAA CCAGCCACGG TGTGGGGAAG 
ATCGCCATGA CCCAGCGCAG ACTCTTCCTG CTGACGGAGG GACGACCCGG ATACGTGGAG 
ATCACCAAAT TCAGAGACAT CGAGGATGTA AGGATTTCCT CTGCCCCGTT CCTCCTCCTG 
AGAATCCCAT CACTGAAGAT CAGGACGTCC CTCAGGAGGG AGTCATTCGA GGCCAATCTG 
AAGTCCGAGT GTGACCTCTG GCACCTGATG ATCAAAGAGA TGTGGGCCGG GAGGAAGATG 
GCGGACCAAC ATAAGGATCC TCAGTTTATG GAGCAGGCTT TGACTAACGC TCTTCTGATG 
GACGCTGTGG TGGGCAGTCT GCAGTCTCAG AAAGCCATCT ACGCTGCCAC TAAACTGGCT 
TACTTTGACA AGATGAAGTC TGAGGTGCCC ATGATGGTCC CCAAAACCAC CTCTGACACC 
CTGAAGCACA AGATCAACCC GTCTCTGGAC CAGACGAGTC CTCAAGCAGT GGACGTTCTG 
CTCTACACTC CAGGTCAGCT GGGTGTGTCA GATGTGGACG GTGGTGGGAA CCCGAAGCTG 
TGGTGTGCTC TGAGTGATGG GAAGCTGCTA GTTTTTGACG CCGCCAGCTG GTCCATGCAG 
CACAACTCTG TTCAAGTCGG GACGTCCCGT CTGACGTGTA TGCTGGCAGT GAATGAGCAG 
CAGCTGTGGA TCGGCTCTAA AGATTCCTTC ATCTACATCA TAAACCCGCG CAGCGTCTCC 
TGCAATAAAC AGCTGACGGA GCATCGCTGT GAGCTCACCG GGCTCGCCCT GGAGGAGAGA 
GCCGACAAAT TCAGCCAGAC GGTGGCGTAC TCCTGCAGTG AGGACTGCAA TGTGATCGTG 
TGGGACGTTT CCTCACTGCA GGTCAGACGG CAGTTCAGAG TGTCCTGCGG CCGACTGCAG 
TCCATGCACG TCCACAACGG CATGCTGTGG TGCTGTGGTC GTGATTGTGT GCTGGAGGTG 
CGCAGGAACG GCTCGGTTCA CCGTCGGATC TCTCTGCCCG ATCACCTGCT GAGCTCACCA 
AAGGGCTTCA GCAGTTTCCT GCTCTTTAAT GAGTCGGAGC AGCTGTGGGC TGGTTTTGCG 
GATGCGGGTG AGCTGTGTGT TTGGCACTGC AGAGACATCA GCAAACCCTT CATCAGGATT 
CAGCTCAAAG ACTGCGCCGC CGTCACCTGC ATGATCAGGG TCAAGAACCA GATCTGGGTC 
GGCGGTTACA ATGGCAGCGT CACAGACAGA CGAGTGCGAG GGAAGATCTA CATAGTAAAC 
ACGGACCGAT ATACAGTGGA GAAAGAGCTG CAGGCCCACG CCGACGCCGT TCAGACCATG 
TGTTCAGCCG AAGACCGATA TGTGCTCAGC GGAGCCGCAC AGGATGATGG GAAAATCGGC 
ATCTGGAAGG TGGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DENND3A ( XM_001338383.7 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_001338419.5 Danio rerio DENN/MADD domain containing 3a (dennd3a), transcript variant X3, mRNA.

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CloneID	ODa150190
Clone ID Related Accession (Same CDS sequence)	XM_001338383.7
Accession Version	XM_001338383.7 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3813bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	DENN domain-containing protein 3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007130.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_001338383.6. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGATA ATGTGCCGTG TGGTCTGCTG GAGGCCTGTG TGCTGCTTGG AGTGTCAAAC 
GAGCGCCTGA AGGAGCTGTG CCCGGGTAAA GATGCAGATG TGTTGGTGGA GGCGGAGGTG 
CTGCAGGTTC ACGCTCCACC GTTTATAACA AAGGATCGCT CTTGTAACGG CGGCGTAGAC 
CAGGGCGTGG CTCCGGCCTT TAGCCGAGTC CAGAAGAGAC GCTCCTTCAA GAAGAAGAAG 
AGAGAGCGGC CGGTGTCAAC CCTCAGCAAC TCCAGTCAAG CCAGTGAATC TGAAGAGCTG 
AGCGTCCCGC AGAACATTGA TCTGATCGCC CTGCCACAGC TCTGCTTTCC TGATGGGTTG 
AGAATCACCA ATGAGAGCAG AGAAGACTCG TATCACTTCC TGGTGTTCAC GGATGTGTTC 
GGGAATCAGA CTCATGGTGT GGTCGCTCAG TACTGCAGAC CTGTACAGTT CCAGCAAGAT 
AATGTGATTT ATGCAAACGG CCATCGAACC AACGAACACA AGCGTCTGTA CACCACATTC 
AGCATATGTG TGATCTCCAA ATATCCCTAT TATAACGCCC TCAGAGACTG CCTGTCCTGT 
TTTCTGTTCC AGATGAAGAC ATCCCGTGTG TCTGACTTTG ATGAGCAGGT GAAGGAGTTT 
TCTGCCAAAC TGGCGCTGGT GCCCATCCCT CCATCTGGAC CACTGCATGT GGTGTTTAAT 
CTCAGACCGC TTCAGATTGA GCTTCCCTCC AGACTGGATG TGGATCGACC CGTAATGGAC 
CTCGATCTCC ATCTGCCATT TCTGTGCTTT AAACCCAAGC AGATCCTGCA GATCATCAGC 
AGTATCCTGA TGGAGCAGAG AGTGGTGTTT CTGTCCACAG ACTGGGCCAA ACTCACACTG 
GTGTCCGAGT GCTTCATGAT CTTTATTCAC CCTCTGCGCT GGCAGCATCC GTTTGTGCCC 
GTCCTGTCCC GACAGATGCT GGACTTCATC ATGGCGCCCA CGGCCTACCT GATGGGCTGC 
CACACGTACC ACTTCCAGGA GGTGGCAGAG GAGATTGAGG ATCTGGTGCT GATAGACATT 
GACCAGGGAA CAGTGTCGTC TTCAACATCA GACAGATTTG ATCTTCCAGA CGTGCCGCTT 
GCAGCCAGAG ATTGTTTTAT ATTCCGCTGT GGGGGTCTGC AGCTCCACTA TGATCTGGAT 
GTGTGCGGCT CCAGCAGTCA GACTGATGTG AATGATCTGC GAGCTCACAG AAGACTCTGG 
CAGCGCAAAC TCAACTCCAG CATCCTCAAC ATCAGCATGG AGCTCATCGT CAACATATTC 
AGTGAGGTTT GTGATTTCCT GAACTATGAG CATCGAGTTT TCAACAGCGA GGAGTTCCTC 
AGGTCCAGAG ACTCCACCGA CTCTGCCTTT TACAGAAAGG TTTTAGACAC TCATATATTC 
CACTCGTTTC TGCGGGAGCG TTTGAACAGA AAGAATGACG CATTCACTCG CATGCAGCTC 
AACATCCGCT CAGAGACTCG CAGGCTGAAG ATTATGACCG AGTCTCCGCG CAGACCCACG 
ATGGAGGAGA TCAACCGTAA ACACCGAGCA GAGAACGGCC TGAATAAGCG TTTGGGGATG 
AGCATGCCGA ACCTGGCGGA CTCTCGATCT CTCACCGTAC CCATCCGACA GATGTCCATG 
ATGTCAGTGA GGATGCCTTT ACAGATGGGT TCCTGGAGTC CTATAAAGCC AGTCAAAACC 
TTCAAACTCC CAGAATTCCC TCCACCACTG GCCTACCACT ATGTACAGAA CTACTACCAC 
GATCTGATTG GGCAGTTGGA CAGATCCATC TCATCTGCTT CCCCAGACGA CTCTGCTCTC 
TTGGCCCGCT ATTATTACCT GCGTGGACTG GTGAATTCTG TGACTGGACG ACATGTGGAC 
GCCCTCGCAG ACTTCCAGAG CTTATATAAG ACGGACATGG AGATTTTTCC TGCGCAGCTT 
CTGAACACTC TGGTGGAGGC TCTGCCTGTG GAGGAAAAGA GGATTGCGGA GCGCCGCACC 
GATCTGAAGC GTCTCATGAG CAGGGTGAGG AGGGAAAACG AAAAGGACCG AGCGCGGCTG 
GTGGACGGCG GGACGGTGAA GAGGTTTGAG CTGCCCAAAA AACACCTGCA AAAGGAGGAT 
TTTGTGCGGC GCGTTCAGGA GTCGGGAATC GTGAAGGATC TGGAGACCAT CAAGAGGCTG 
TTCGAGGCAC TCACTCTGGG TCAGGTGAAG CAGGTGGATC CAGAAACCTT CCGCTTCTTC 
TACAACTTCT GGAAGGAAAC GGAAGCGGCG GCGCAGGACG TGGATCTGCC GGCCGTCGTG 
TTGGAGCATC TGGAGAACGA TGAGTGTGTT TACAAAGTCT CGTCATCTGT AAAAACCAGC 
CACGGTGTGG GGAAGATCGC CATGACCCAG CGCAGACTCT TCCTGCTGAC GGAGGGACGA 
CCCGGATACG TGGAGATCAC CAAATTCAGA GACATCGAGG ATGTAAGGAT TTCCTCTGCC 
CCGTTCCTCC TCCTGAGAAT CCCATCACTG AAGATCAGGA CGTCCCTCAG GAGGGAGTCA 
TTCGAGGCCA ATCTGAAGTC CGAGTGTGAC CTCTGGCACC TGATGATCAA AGAGATGTGG 
GCCGGGAGGA AGATGGCGGA CCAACATAAG GATCCTCAGT TTATGGAGCA GGCTTTGACT 
AACGCTCTTC TGATGGACGC TGTGGTGGGC AGTCTGCAGT CTCAGAAAGC CATCTACGCT 
GCCACTAAAC TGGCTTACTT TGACAAGATG AAGTCTGAGG TGCCCATGAT GGTCCCCAAA 
ACCACCTCTG ACACCCTGAA GCACAAGATC AACCCGTCTC TGGACCAGAC GAGTCCTCAA 
GCAGTGGACG TTCTGCTCTA CACTCCAGGT CAGCTGGGTG TGTCAGATGT GGACGGTGGT 
GGGAACCCGA AGCTGTGGTG TGCTCTGAGT GATGGGAAGC TGCTAGTTTT TGACGCCGCC 
AGCTGGTCCA TGCAGCACAA CTCTGTTCAA GTCGGGACGT CCCGTCTGAC GTGTATGCTG 
GCAGTGAATG AGCAGCAGCT GTGGATCGGC TCTAAAGATT CCTTCATCTA CATCATAAAC 
CCGCGCAGCG TCTCCTGCAA TAAACAGCTG ACGGAGCATC GCTGTGAGCT CACCGGGCTC 
GCCCTGGAGG AGAGAGCCGA CAAATTCAGC CAGACGGTGG CGTACTCCTG CAGTGAGGAC 
TGCAATGTGA TCGTGTGGGA CGTTTCCTCA CTGCAGGTCA GACGGCAGTT CAGAGTGTCC 
TGCGGCCGAC TGCAGTCCAT GCACGTCCAC AACGGCATGC TGTGGTGCTG TGGTCGTGAT 
TGTGTGCTGG AGGTGCGCAG GAACGGCTCG GTTCACCGTC GGATCTCTCT GCCCGATCAC 
CTGCTGAGCT CACCAAAGGG CTTCAGCAGT TTCCTGCTCT TTAATGAGTC GGAGCAGCTG 
TGGGCTGGTT TTGCGGATGC GGGTGAGCTG TGTGTTTGGC ACTGCAGAGA CATCAGCAAA 
CCCTTCATCA GGATTCAGCT CAAAGACTGC GCCGCCGTCA CCTGCATGAT CAGGGTCAAG 
AACCAGATCT GGGTCGGCGG TTACAATGGC AGCGTCACAG ACAGACGAGT GCGAGGGAAG 
ATCTACATAG TAAACACGGA CCGATATACA GTGGAGAAAG AGCTGCAGGC CCACGCCGAC 
GCCGTTCAGA CCATGTGTTC AGCCGAAGAC CGATATGTGC TCAGCGGAGC CGCACAGGAT 
GATGGGAAAA TCGGCATCTG GAAGGTGGAG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001338419.5
CDS	391..4203
Translation	MADNVPCGLLEACVLLGVSNERLKELCPGKDADVLVEAEVLQVHAPPFITKDRSCNGGVDQGVAPAFSRVQKRRSFKKKKRERPVSTLSNSSQASESEELSVPQNIDLIALPQLCFPDGLRITNESREDSYHFLVFTDVFGNQTHGVVAQYCRPVQFQQDNVIYANGHRTNEHKRLYTTFSICVISKYPYYNALRDCLSCFLFQMKTSRVSDFDEQVKEFSAKLALVPIPPSGPLHVVFNLRPLQIELPSRLDVDRPVMDLDLHLPFLCFKPKQILQIISSILMEQRVVFLSTDWAKLTLVSECFMIFIHPLRWQHPFVPVLSRQMLDFIMAPTAYLMGCHTYHFQEVAEEIEDLVLIDIDQGTVSSSTSDRFDLPDVPLAARDCFIFRCGGLQLHYDLDVCGSSSQTDVNDLRAHRRLWQRKLNSSILNISMELIVNIFSEVCDFLNYEHRVFNSEEFLRSRDSTDSAFYRKVLDTHIFHSFLRERLNRKNDAFTRMQLNIRSETRRLKIMTESPRRPTMEEINRKHRAENGLNKRLGMSMPNLADSRSLTVPIRQMSMMSVRMPLQMGSWSPIKPVKTFKLPEFPPPLAYHYVQNYYHDLIGQLDRSISSASPDDSALLARYYYLRGLVNSVTGRHVDALADFQSLYKTDMEIFPAQLLNTLVEALPVEEKRIAERRTDLKRLMSRVRRENEKDRARLVDGGTVKRFELPKKHLQKEDFVRRVQESGIVKDLETIKRLFEALTLGQVKQVDPETFRFFYNFWKETEAAAQDVDLPAVVLEHLENDECVYKVSSSVKTSHGVGKIAMTQRRLFLLTEGRPGYVEITKFRDIEDVRISSAPFLLLRIPSLKIRTSLRRESFEANLKSECDLWHLMIKEMWAGRKMADQHKDPQFMEQALTNALLMDAVVGSLQSQKAIYAATKLAYFDKMKSEVPMMVPKTTSDTLKHKINPSLDQTSPQAVDVLLYTPGQLGVSDVDGGGNPKLWCALSDGKLLVFDAASWSMQHNSVQVGTSRLTCMLAVNEQQLWIGSKDSFIYIINPRSVSCNKQLTEHRCELTGLALEERADKFSQTVAYSCSEDCNVIVWDVSSLQVRRQFRVSCGRLQSMHVHNGMLWCCGRDCVLEVRRNGSVHRRISLPDHLLSSPKGFSSFLLFNESEQLWAGFADAGELCVWHCRDISKPFIRIQLKDCAAVTCMIRVKNQIWVGGYNGSVTDRRVRGKIYIVNTDRYTVEKELQAHADAVQTMCSAEDRYVLSGAAQDDGKIGIWKVE

Target ORF information:

RefSeq Version	XM_001338383.7
Organism	Danio rerio(zebrafish)
Definition	Danio rerio DENN/MADD domain containing 3a (dennd3a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001338383.7

ORF Insert Sequence:

ATGGCTGATA ATGTGCCGTG TGGTCTGCTG GAGGCCTGTG TGCTGCTTGG AGTGTCAAAC 
GAGCGCCTGA AGGAGCTGTG CCCGGGTAAA GATGCAGATG TGTTGGTGGA GGCGGAGGTG 
CTGCAGGTTC ACGCTCCACC GTTTATAACA AAGGATCGCT CTTGTAACGG CGGCGTAGAC 
CAGGGCGTGG CTCCGGCCTT TAGCCGAGTC CAGAAGAGAC GCTCCTTCAA GAAGAAGAAG 
AGAGAGCGGC CGGTGTCAAC CCTCAGCAAC TCCAGTCAAG CCAGTGAATC TGAAGAGCTG 
AGCGTCCCGC AGAACATTGA TCTGATCGCC CTGCCACAGC TCTGCTTTCC TGATGGGTTG 
AGAATCACCA ATGAGAGCAG AGAAGACTCG TATCACTTCC TGGTGTTCAC GGATGTGTTC 
GGGAATCAGA CTCATGGTGT GGTCGCTCAG TACTGCAGAC CTGTACAGTT CCAGCAAGAT 
AATGTGATTT ATGCAAACGG CCATCGAACC AACGAACACA AGCGTCTGTA CACCACATTC 
AGCATATGTG TGATCTCCAA ATATCCCTAT TATAACGCCC TCAGAGACTG CCTGTCCTGT 
TTTCTGTTCC AGATGAAGAC ATCCCGTGTG TCTGACTTTG ATGAGCAGGT GAAGGAGTTT 
TCTGCCAAAC TGGCGCTGGT GCCCATCCCT CCATCTGGAC CACTGCATGT GGTGTTTAAT 
CTCAGACCGC TTCAGATTGA GCTTCCCTCC AGACTGGATG TGGATCGACC CGTAATGGAC 
CTCGATCTCC ATCTGCCATT TCTGTGCTTT AAACCCAAGC AGATCCTGCA GATCATCAGC 
AGTATCCTGA TGGAGCAGAG AGTGGTGTTT CTGTCCACAG ACTGGGCCAA ACTCACACTG 
GTGTCCGAGT GCTTCATGAT CTTTATTCAC CCTCTGCGCT GGCAGCATCC GTTTGTGCCC 
GTCCTGTCCC GACAGATGCT GGACTTCATC ATGGCGCCCA CGGCCTACCT GATGGGCTGC 
CACACGTACC ACTTCCAGGA GGTGGCAGAG GAGATTGAGG ATCTGGTGCT GATAGACATT 
GACCAGGGAA CAGTGTCGTC TTCAACATCA GACAGATTTG ATCTTCCAGA CGTGCCGCTT 
GCAGCCAGAG ATTGTTTTAT ATTCCGCTGT GGGGGTCTGC AGCTCCACTA TGATCTGGAT 
GTGTGCGGCT CCAGCAGTCA GACTGATGTG AATGATCTGC GAGCTCACAG AAGACTCTGG 
CAGCGCAAAC TCAACTCCAG CATCCTCAAC ATCAGCATGG AGCTCATCGT CAACATATTC 
AGTGAGGTTT GTGATTTCCT GAACTATGAG CATCGAGTTT TCAACAGCGA GGAGTTCCTC 
AGGTCCAGAG ACTCCACCGA CTCTGCCTTT TACAGAAAGG TTTTAGACAC TCATATATTC 
CACTCGTTTC TGCGGGAGCG TTTGAACAGA AAGAATGACG CATTCACTCG CATGCAGCTC 
AACATCCGCT CAGAGACTCG CAGGCTGAAG ATTATGACCG AGTCTCCGCG CAGACCCACG 
ATGGAGGAGA TCAACCGTAA ACACCGAGCA GAGAACGGCC TGAATAAGCG TTTGGGGATG 
AGCATGCCGA ACCTGGCGGA CTCTCGATCT CTCACCGTAC CCATCCGACA GATGTCCATG 
ATGTCAGTGA GGATGCCTTT ACAGATGGGT TCCTGGAGTC CTATAAAGCC AGTCAAAACC 
TTCAAACTCC CAGAATTCCC TCCACCACTG GCCTACCACT ATGTACAGAA CTACTACCAC 
GATCTGATTG GGCAGTTGGA CAGATCCATC TCATCTGCTT CCCCAGACGA CTCTGCTCTC 
TTGGCCCGCT ATTATTACCT GCGTGGACTG GTGAATTCTG TGACTGGACG ACATGTGGAC 
GCCCTCGCAG ACTTCCAGAG CTTATATAAG ACGGACATGG AGATTTTTCC TGCGCAGCTT 
CTGAACACTC TGGTGGAGGC TCTGCCTGTG GAGGAAAAGA GGATTGCGGA GCGCCGCACC 
GATCTGAAGC GTCTCATGAG CAGGGTGAGG AGGGAAAACG AAAAGGACCG AGCGCGGCTG 
GTGGACGGCG GGACGGTGAA GAGGTTTGAG CTGCCCAAAA AACACCTGCA AAAGGAGGAT 
TTTGTGCGGC GCGTTCAGGA GTCGGGAATC GTGAAGGATC TGGAGACCAT CAAGAGGCTG 
TTCGAGGCAC TCACTCTGGG TCAGGTGAAG CAGGTGGATC CAGAAACCTT CCGCTTCTTC 
TACAACTTCT GGAAGGAAAC GGAAGCGGCG GCGCAGGACG TGGATCTGCC GGCCGTCGTG 
TTGGAGCATC TGGAGAACGA TGAGTGTGTT TACAAAGTCT CGTCATCTGT AAAAACCAGC 
CACGGTGTGG GGAAGATCGC CATGACCCAG CGCAGACTCT TCCTGCTGAC GGAGGGACGA 
CCCGGATACG TGGAGATCAC CAAATTCAGA GACATCGAGG ATGTAAGGAT TTCCTCTGCC 
CCGTTCCTCC TCCTGAGAAT CCCATCACTG AAGATCAGGA CGTCCCTCAG GAGGGAGTCA 
TTCGAGGCCA ATCTGAAGTC CGAGTGTGAC CTCTGGCACC TGATGATCAA AGAGATGTGG 
GCCGGGAGGA AGATGGCGGA CCAACATAAG GATCCTCAGT TTATGGAGCA GGCTTTGACT 
AACGCTCTTC TGATGGACGC TGTGGTGGGC AGTCTGCAGT CTCAGAAAGC CATCTACGCT 
GCCACTAAAC TGGCTTACTT TGACAAGATG AAGTCTGAGG TGCCCATGAT GGTCCCCAAA 
ACCACCTCTG ACACCCTGAA GCACAAGATC AACCCGTCTC TGGACCAGAC GAGTCCTCAA 
GCAGTGGACG TTCTGCTCTA CACTCCAGGT CAGCTGGGTG TGTCAGATGT GGACGGTGGT 
GGGAACCCGA AGCTGTGGTG TGCTCTGAGT GATGGGAAGC TGCTAGTTTT TGACGCCGCC 
AGCTGGTCCA TGCAGCACAA CTCTGTTCAA GTCGGGACGT CCCGTCTGAC GTGTATGCTG 
GCAGTGAATG AGCAGCAGCT GTGGATCGGC TCTAAAGATT CCTTCATCTA CATCATAAAC 
CCGCGCAGCG TCTCCTGCAA TAAACAGCTG ACGGAGCATC GCTGTGAGCT CACCGGGCTC 
GCCCTGGAGG AGAGAGCCGA CAAATTCAGC CAGACGGTGG CGTACTCCTG CAGTGAGGAC 
TGCAATGTGA TCGTGTGGGA CGTTTCCTCA CTGCAGGTCA GACGGCAGTT CAGAGTGTCC 
TGCGGCCGAC TGCAGTCCAT GCACGTCCAC AACGGCATGC TGTGGTGCTG TGGTCGTGAT 
TGTGTGCTGG AGGTGCGCAG GAACGGCTCG GTTCACCGTC GGATCTCTCT GCCCGATCAC 
CTGCTGAGCT CACCAAAGGG CTTCAGCAGT TTCCTGCTCT TTAATGAGTC GGAGCAGCTG 
TGGGCTGGTT TTGCGGATGC GGGTGAGCTG TGTGTTTGGC ACTGCAGAGA CATCAGCAAA 
CCCTTCATCA GGATTCAGCT CAAAGACTGC GCCGCCGTCA CCTGCATGAT CAGGGTCAAG 
AACCAGATCT GGGTCGGCGG TTACAATGGC AGCGTCACAG ACAGACGAGT GCGAGGGAAG 
ATCTACATAG TAAACACGGA CCGATATACA GTGGAGAAAG AGCTGCAGGC CCACGCCGAC 
GCCGTTCAGA CCATGTGTTC AGCCGAAGAC CGATATGTGC TCAGCGGAGC CGCACAGGAT 
GATGGGAAAA TCGGCATCTG GAAGGTGGAG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DENND3A ( XM_005169976.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005170033.1 Danio rerio DENN/MADD domain containing 3a (dennd3a), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa23720
Clone ID Related Accession (Same CDS sequence)	XM_005169976.3 , XM_001338383.6
Accession Version	XM_005169976.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3813bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	DENN domain-containing protein 3 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007130.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005169976.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGATA ATGTGCCGTG TGGTCTGCTG GAGGCCTGTG TGCTGCTTGG AGTGTCAAAC 
GAGCGCCTGA AGGAGCTGTG CCCGGGTAAA GATGCAGATG TGTTGGTGGA GGCGGAGGTG 
CTGCAGGTTC ACGCTCCACC GTTTATAACA AAGGATCGCT CTTGTAACGG CGGCGTAGAC 
CAGGGCGTGG CTCCGGCCTT TAGCCGAGTC CAGAAGAGAC GCTCCTTCAA GAAGAAGAAG 
AGAGAGCGGC CGGTGTCAAC CCTCAGCAAC TCCAGTCAAG CCAGTGAATC TGAAGAGCTG 
AGCGTCCCGC AGAACATTGA TCTGATCGCC CTGCCACAGC TCTGCTTTCC TGATGGGTTG 
AGAATCACCA ATGAGAGCAG AGAAGACTCG TATCACTTCC TGGTGTTCAC GGATGTGTTC 
GGGAATCAGA CTCATGGTGT GGTCGCTCAG TACTGCAGAC CTGTACAGTT CCAGCAAGAT 
AATGTGATTT ATGCAAACGG CCATCGAACC AACGAACACA AGCGTCTGTA CACCACATTC 
AGCATATGTG TGATCTCCAA ATATCCCTAT TATAACGCCC TCAGAGACTG CCTGTCCTGT 
TTTCTGTTCC AGATGAAGAC ATCCCGTGTG TCTGACTTTG ATGAGCAGGT GAAGGAGTTT 
TCTGCCAAAC TGGCGCTGGT GCCCATCCCT CCATCTGGAC CACTGCATGT GGTGTTTAAT 
CTCAGACCGC TTCAGATTGA GCTTCCCTCC AGACTGGATG TGGATCGACC CGTAATGGAC 
CTCGATCTCC ATCTGCCATT TCTGTGCTTT AAACCCAAGC AGATCCTGCA GATCATCAGC 
AGTATCCTGA TGGAGCAGAG AGTGGTGTTT CTGTCCACAG ACTGGGCCAA ACTCACACTG 
GTGTCCGAGT GCTTCATGAT CTTTATTCAC CCTCTGCGCT GGCAGCATCC GTTTGTGCCC 
GTCCTGTCCC GACAGATGCT GGACTTCATC ATGGCGCCCA CGGCCTACCT GATGGGCTGC 
CACACGTACC ACTTCCAGGA GGTGGCAGAG GAGATTGAGG ATCTGGTGCT GATAGACATT 
GACCAGGGAA CAGTGTCGTC TTCAACATCA GACAGATTTG ATCTTCCAGA CGTGCCGCTT 
GCAGCCAGAG ATTGTTTTAT ATTCCGCTGT GGGGGTCTGC AGCTCCACTA TGATCTGGAT 
GTGTGCGGCT CCAGCAGTCA GACTGATGTG AATGATCTGC GAGCTCACAG AAGACTCTGG 
CAGCGCAAAC TCAACTCCAG CATCCTCAAC ATCAGCATGG AGCTCATCGT CAACATATTC 
AGTGAGGTTT GTGATTTCCT GAACTATGAG CATCGAGTTT TCAACAGCGA GGAGTTCCTC 
AGGTCCAGAG ACTCCACCGA CTCTGCCTTT TACAGAAAGG TTTTAGACAC TCATATATTC 
CACTCGTTTC TGCGGGAGCG TTTGAACAGA AAGAATGACG CATTCACTCG CATGCAGCTC 
AACATCCGCT CAGAGACTCG CAGGCTGAAG ATTATGACCG AGTCTCCGCG CAGACCCACG 
ATGGAGGAGA TCAACCGTAA ACACCGAGCA GAGAACGGCC TGAATAAGCG TTTGGGGATG 
AGCATGCCGA ACCTGGCGGA CTCTCGATGT CTCACCGTAC CCATCCGACA GATGTCCATG 
ATGTCAGTGA GGATGCCTTT ACAGATGGGT TCCTGGAGTC CTATAAAGCC AGTCAAAACC 
TTCAAACTCC CAGAATTCCC TCCACCACTG GCCTACCACT ATGTACAGAA CTACTACCAC 
GATCTGATTG GGCAGTTGGA CAGATCCATC TCATCTGCTT CCCCAGACGA CTCTGCTCTC 
TTGGCCCGCT ATTATTACCT GCGTGGACTG GTGAATTCTG TGACTGGACG ACATGTGGAC 
GCCCTCGCAG ACTTCCAGAG CTTATATAAG ACGGACATGG AGATTTTTCC TGCGCAGCTT 
CTGAACACTC TGGTGGAGGC TCTGCCTGTG GAGGAAAAGA GGATTGCGGA GCGCCGCACC 
GATCTGAAGC GTCTCATGAG CAGGGTGAGG AGGGAAAACG AAAAGGACCG AGCGCGGCTG 
GTGGACGGCG GGACGGTGAA GAGGTTTGAG CTGCCCAAAA AACACCTGCA AAAGGAGGAT 
TTTGTGCGGC GCGTTCAGGA GTCGGGAATC GTGAAGGATC TGGAGACCAT CAAGAGGCTG 
TTCGAGGCAC TCACTCTGGG TCAGGTGAAG CAGGTGGATC CAGAAACCTT CCGCTTCTTC 
TACAACTTCT GGAAGGAAAC GGAAGCGGCG GCGCAGGACG TGGATCTGCC GGCCGTCGTG 
TTGGAGCATC TGGAGAACGA TGAGTGTGTT TACAAAGTCT CGTCATCTGT AAAAACCAGC 
CACGGTGTGG GGAAGATCGC CATGACCCAG CGCAGACTCT TCCTGCTGAC GGAGGGACGA 
CCCGGATACG TGGAGATCAC CAAATTCAGA GACATCGAGG ATGTAAGGAT TTCCTCTGCC 
CCGTTCCTCC TCCTGAGAAT CCCATCACTG AAGATCAGGA CGTCCCTCAG GAGGGAGTCA 
TTCGAGGCCA ATCTGAAGTC CGAGTGTGAC CTCTGGCACC TGATGATCAA AGAGATGTGG 
GCCGGGAGGA AGATGGCGGA CCAACATAAG GATCCTCAGT TTATGGAGCA GGCTTTGACT 
AACGCTCTTC TGATGGACGC TGTGGTGGGC AGTCTGCAGT CTCAGAAAGC CATCTACGCT 
GCCACTAAAC TGGCTTACTT TGACAAGATG AAGTCTGAGG TGCCCATGAT GGTCCCCAAA 
ACCACCTCTG ACACCCTGAA GCACAAGATC AACCCGTCTC TGGACCAGAC GAGTCCTCAA 
GCAGTGGACG TTCTGCTCTA CACTCCAGGT CAGCTGGGTG TGTCAGATGT GGACGGTGGT 
GGGAACCCGA AGCTGTGGTG TGCTCTGAGT GATGGGAAGC TGCTAGTTTT TGACGCCGCC 
AGCTGGTCCA TGCAGCACAA CTCTGTTCAA GTCGGGACGT CCCGTCTGAC GTGTATGCTG 
GCAGTGAATG AGCAGCAGCT GTGGATCGGC TCTAAAGATT CCTTCATCTA CATCATAAAC 
CCGCGCAGCG TCTCCTGCAA TAAACAGCTG ACGGAGCATC GCTGTGAGCT CACCGGGCTC 
GCCCTGGAGG AGAGAGCCGA CAAATTCAGC CAGACGGTGG CGTACTCCTG CAGTGAGGAC 
TGCAATGTGA TCGTGTGGGA CGTTTCCTCA CTGCAGGTCA GACGGCAGTT CAGAGTGTCC 
TGCGGCCGAC TGCAGTCCAT GCACGTCCAC AACGGCATGC TGTGGTGCTG TGGTCGTGAT 
TGTGTGCTGG AGGTGCGCAG GAACGGCTCG GTTCACCGTC GGATCTCTCT GCCCGATCAC 
CTGCTGAGCT CACCAAAGGG CTTCAGCAGT TTCCTGCTCT TTAATGAGTC GGAGCAGCTG 
TGGGCTGGTT TTGCGGATGC GGGTGAGCTG TGTGTTTGGC ACTGCAGAGA CATCAGCAAA 
CCCTTCATCA GGATTCAGCT CAAAGACTGC GCCGCCGTCA CCTGCATGAT CAGGGTCAAG 
AACCAGATCT GGGTCGGCGG TTACAATGGC AGCGTCACAG ACAGACGAGT GCGAGGGAAG 
ATCTACATAG TAAACACGGA CCGATATACA GTGGAGAAAG AGCTGCAGGC CCACGCCGAC 
GCCGTTCAGA CCATGTGTTC AGCCGAAGAC CGATATGTGC TCAGCGGAGC CGCACAGGAT 
GATGGGAAAA TCGGCATCTG GAAGGTGGAG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005170033.1
CDS	392..4204
Translation	MADNVPCGLLEACVLLGVSNERLKELCPGKDADVLVEAEVLQVHAPPFITKDRSCNGGVDQGVAPAFSRVQKRRSFKKKKRERPVSTLSNSSQASESEELSVPQNIDLIALPQLCFPDGLRITNESREDSYHFLVFTDVFGNQTHGVVAQYCRPVQFQQDNVIYANGHRTNEHKRLYTTFSICVISKYPYYNALRDCLSCFLFQMKTSRVSDFDEQVKEFSAKLALVPIPPSGPLHVVFNLRPLQIELPSRLDVDRPVMDLDLHLPFLCFKPKQILQIISSILMEQRVVFLSTDWAKLTLVSECFMIFIHPLRWQHPFVPVLSRQMLDFIMAPTAYLMGCHTYHFQEVAEEIEDLVLIDIDQGTVSSSTSDRFDLPDVPLAARDCFIFRCGGLQLHYDLDVCGSSSQTDVNDLRAHRRLWQRKLNSSILNISMELIVNIFSEVCDFLNYEHRVFNSEEFLRSRDSTDSAFYRKVLDTHIFHSFLRERLNRKNDAFTRMQLNIRSETRRLKIMTESPRRPTMEEINRKHRAENGLNKRLGMSMPNLADSRCLTVPIRQMSMMSVRMPLQMGSWSPIKPVKTFKLPEFPPPLAYHYVQNYYHDLIGQLDRSISSASPDDSALLARYYYLRGLVNSVTGRHVDALADFQSLYKTDMEIFPAQLLNTLVEALPVEEKRIAERRTDLKRLMSRVRRENEKDRARLVDGGTVKRFELPKKHLQKEDFVRRVQESGIVKDLETIKRLFEALTLGQVKQVDPETFRFFYNFWKETEAAAQDVDLPAVVLEHLENDECVYKVSSSVKTSHGVGKIAMTQRRLFLLTEGRPGYVEITKFRDIEDVRISSAPFLLLRIPSLKIRTSLRRESFEANLKSECDLWHLMIKEMWAGRKMADQHKDPQFMEQALTNALLMDAVVGSLQSQKAIYAATKLAYFDKMKSEVPMMVPKTTSDTLKHKINPSLDQTSPQAVDVLLYTPGQLGVSDVDGGGNPKLWCALSDGKLLVFDAASWSMQHNSVQVGTSRLTCMLAVNEQQLWIGSKDSFIYIINPRSVSCNKQLTEHRCELTGLALEERADKFSQTVAYSCSEDCNVIVWDVSSLQVRRQFRVSCGRLQSMHVHNGMLWCCGRDCVLEVRRNGSVHRRISLPDHLLSSPKGFSSFLLFNESEQLWAGFADAGELCVWHCRDISKPFIRIQLKDCAAVTCMIRVKNQIWVGGYNGSVTDRRVRGKIYIVNTDRYTVEKELQAHADAVQTMCSAEDRYVLSGAAQDDGKIGIWKVE

Target ORF information:

RefSeq Version	XM_005169976.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio DENN/MADD domain containing 3a (dennd3a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005169976.3

ORF Insert Sequence:

ATGGCTGATA ATGTGCCGTG TGGTCTGCTG GAGGCCTGTG TGCTGCTTGG AGTGTCAAAC 
GAGCGCCTGA AGGAGCTGTG CCCGGGTAAA GATGCAGATG TGTTGGTGGA GGCGGAGGTG 
CTGCAGGTTC ACGCTCCACC GTTTATAACA AAGGATCGCT CTTGTAACGG CGGCGTAGAC 
CAGGGCGTGG CTCCGGCCTT TAGCCGAGTC CAGAAGAGAC GCTCCTTCAA GAAGAAGAAG 
AGAGAGCGGC CGGTGTCAAC CCTCAGCAAC TCCAGTCAAG CCAGTGAATC TGAAGAGCTG 
AGCGTCCCGC AGAACATTGA TCTGATCGCC CTGCCACAGC TCTGCTTTCC TGATGGGTTG 
AGAATCACCA ATGAGAGCAG AGAAGACTCG TATCACTTCC TGGTGTTCAC GGATGTGTTC 
GGGAATCAGA CTCATGGTGT GGTCGCTCAG TACTGCAGAC CTGTACAGTT CCAGCAAGAT 
AATGTGATTT ATGCAAACGG CCATCGAACC AACGAACACA AGCGTCTGTA CACCACATTC 
AGCATATGTG TGATCTCCAA ATATCCCTAT TATAACGCCC TCAGAGACTG CCTGTCCTGT 
TTTCTGTTCC AGATGAAGAC ATCCCGTGTG TCTGACTTTG ATGAGCAGGT GAAGGAGTTT 
TCTGCCAAAC TGGCGCTGGT GCCCATCCCT CCATCTGGAC CACTGCATGT GGTGTTTAAT 
CTCAGACCGC TTCAGATTGA GCTTCCCTCC AGACTGGATG TGGATCGACC CGTAATGGAC 
CTCGATCTCC ATCTGCCATT TCTGTGCTTT AAACCCAAGC AGATCCTGCA GATCATCAGC 
AGTATCCTGA TGGAGCAGAG AGTGGTGTTT CTGTCCACAG ACTGGGCCAA ACTCACACTG 
GTGTCCGAGT GCTTCATGAT CTTTATTCAC CCTCTGCGCT GGCAGCATCC GTTTGTGCCC 
GTCCTGTCCC GACAGATGCT GGACTTCATC ATGGCGCCCA CGGCCTACCT GATGGGCTGC 
CACACGTACC ACTTCCAGGA GGTGGCAGAG GAGATTGAGG ATCTGGTGCT GATAGACATT 
GACCAGGGAA CAGTGTCGTC TTCAACATCA GACAGATTTG ATCTTCCAGA CGTGCCGCTT 
GCAGCCAGAG ATTGTTTTAT ATTCCGCTGT GGGGGTCTGC AGCTCCACTA TGATCTGGAT 
GTGTGCGGCT CCAGCAGTCA GACTGATGTG AATGATCTGC GAGCTCACAG AAGACTCTGG 
CAGCGCAAAC TCAACTCCAG CATCCTCAAC ATCAGCATGG AGCTCATCGT CAACATATTC 
AGTGAGGTTT GTGATTTCCT GAACTATGAG CATCGAGTTT TCAACAGCGA GGAGTTCCTC 
AGGTCCAGAG ACTCCACCGA CTCTGCCTTT TACAGAAAGG TTTTAGACAC TCATATATTC 
CACTCGTTTC TGCGGGAGCG TTTGAACAGA AAGAATGACG CATTCACTCG CATGCAGCTC 
AACATCCGCT CAGAGACTCG CAGGCTGAAG ATTATGACCG AGTCTCCGCG CAGACCCACG 
ATGGAGGAGA TCAACCGTAA ACACCGAGCA GAGAACGGCC TGAATAAGCG TTTGGGGATG 
AGCATGCCGA ACCTGGCGGA CTCTCGATGT CTCACCGTAC CCATCCGACA GATGTCCATG 
ATGTCAGTGA GGATGCCTTT ACAGATGGGT TCCTGGAGTC CTATAAAGCC AGTCAAAACC 
TTCAAACTCC CAGAATTCCC TCCACCACTG GCCTACCACT ATGTACAGAA CTACTACCAC 
GATCTGATTG GGCAGTTGGA CAGATCCATC TCATCTGCTT CCCCAGACGA CTCTGCTCTC 
TTGGCCCGCT ATTATTACCT GCGTGGACTG GTGAATTCTG TGACTGGACG ACATGTGGAC 
GCCCTCGCAG ACTTCCAGAG CTTATATAAG ACGGACATGG AGATTTTTCC TGCGCAGCTT 
CTGAACACTC TGGTGGAGGC TCTGCCTGTG GAGGAAAAGA GGATTGCGGA GCGCCGCACC 
GATCTGAAGC GTCTCATGAG CAGGGTGAGG AGGGAAAACG AAAAGGACCG AGCGCGGCTG 
GTGGACGGCG GGACGGTGAA GAGGTTTGAG CTGCCCAAAA AACACCTGCA AAAGGAGGAT 
TTTGTGCGGC GCGTTCAGGA GTCGGGAATC GTGAAGGATC TGGAGACCAT CAAGAGGCTG 
TTCGAGGCAC TCACTCTGGG TCAGGTGAAG CAGGTGGATC CAGAAACCTT CCGCTTCTTC 
TACAACTTCT GGAAGGAAAC GGAAGCGGCG GCGCAGGACG TGGATCTGCC GGCCGTCGTG 
TTGGAGCATC TGGAGAACGA TGAGTGTGTT TACAAAGTCT CGTCATCTGT AAAAACCAGC 
CACGGTGTGG GGAAGATCGC CATGACCCAG CGCAGACTCT TCCTGCTGAC GGAGGGACGA 
CCCGGATACG TGGAGATCAC CAAATTCAGA GACATCGAGG ATGTAAGGAT TTCCTCTGCC 
CCGTTCCTCC TCCTGAGAAT CCCATCACTG AAGATCAGGA CGTCCCTCAG GAGGGAGTCA 
TTCGAGGCCA ATCTGAAGTC CGAGTGTGAC CTCTGGCACC TGATGATCAA AGAGATGTGG 
GCCGGGAGGA AGATGGCGGA CCAACATAAG GATCCTCAGT TTATGGAGCA GGCTTTGACT 
AACGCTCTTC TGATGGACGC TGTGGTGGGC AGTCTGCAGT CTCAGAAAGC CATCTACGCT 
GCCACTAAAC TGGCTTACTT TGACAAGATG AAGTCTGAGG TGCCCATGAT GGTCCCCAAA 
ACCACCTCTG ACACCCTGAA GCACAAGATC AACCCGTCTC TGGACCAGAC GAGTCCTCAA 
GCAGTGGACG TTCTGCTCTA CACTCCAGGT CAGCTGGGTG TGTCAGATGT GGACGGTGGT 
GGGAACCCGA AGCTGTGGTG TGCTCTGAGT GATGGGAAGC TGCTAGTTTT TGACGCCGCC 
AGCTGGTCCA TGCAGCACAA CTCTGTTCAA GTCGGGACGT CCCGTCTGAC GTGTATGCTG 
GCAGTGAATG AGCAGCAGCT GTGGATCGGC TCTAAAGATT CCTTCATCTA CATCATAAAC 
CCGCGCAGCG TCTCCTGCAA TAAACAGCTG ACGGAGCATC GCTGTGAGCT CACCGGGCTC 
GCCCTGGAGG AGAGAGCCGA CAAATTCAGC CAGACGGTGG CGTACTCCTG CAGTGAGGAC 
TGCAATGTGA TCGTGTGGGA CGTTTCCTCA CTGCAGGTCA GACGGCAGTT CAGAGTGTCC 
TGCGGCCGAC TGCAGTCCAT GCACGTCCAC AACGGCATGC TGTGGTGCTG TGGTCGTGAT 
TGTGTGCTGG AGGTGCGCAG GAACGGCTCG GTTCACCGTC GGATCTCTCT GCCCGATCAC 
CTGCTGAGCT CACCAAAGGG CTTCAGCAGT TTCCTGCTCT TTAATGAGTC GGAGCAGCTG 
TGGGCTGGTT TTGCGGATGC GGGTGAGCTG TGTGTTTGGC ACTGCAGAGA CATCAGCAAA 
CCCTTCATCA GGATTCAGCT CAAAGACTGC GCCGCCGTCA CCTGCATGAT CAGGGTCAAG 
AACCAGATCT GGGTCGGCGG TTACAATGGC AGCGTCACAG ACAGACGAGT GCGAGGGAAG 
ATCTACATAG TAAACACGGA CCGATATACA GTGGAGAAAG AGCTGCAGGC CCACGCCGAC 
GCCGTTCAGA CCATGTGTTC AGCCGAAGAC CGATATGTGC TCAGCGGAGC CGCACAGGAT 
GATGGGAAAA TCGGCATCTG GAAGGTGGAG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DENND3A ( XM_001338383.6 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_001338419.4 Danio rerio DENN/MADD domain containing 3a (dennd3a), transcript variant X1, mRNA.

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CloneID	ODa23720
Clone ID Related Accession (Same CDS sequence)	XM_005169976.3 , XM_001338383.6
Accession Version	XM_001338383.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3813bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	DENN domain-containing protein 3 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007130.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_001338383.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGATA ATGTGCCGTG TGGTCTGCTG GAGGCCTGTG TGCTGCTTGG AGTGTCAAAC 
GAGCGCCTGA AGGAGCTGTG CCCGGGTAAA GATGCAGATG TGTTGGTGGA GGCGGAGGTG 
CTGCAGGTTC ACGCTCCACC GTTTATAACA AAGGATCGCT CTTGTAACGG CGGCGTAGAC 
CAGGGCGTGG CTCCGGCCTT TAGCCGAGTC CAGAAGAGAC GCTCCTTCAA GAAGAAGAAG 
AGAGAGCGGC CGGTGTCAAC CCTCAGCAAC TCCAGTCAAG CCAGTGAATC TGAAGAGCTG 
AGCGTCCCGC AGAACATTGA TCTGATCGCC CTGCCACAGC TCTGCTTTCC TGATGGGTTG 
AGAATCACCA ATGAGAGCAG AGAAGACTCG TATCACTTCC TGGTGTTCAC GGATGTGTTC 
GGGAATCAGA CTCATGGTGT GGTCGCTCAG TACTGCAGAC CTGTACAGTT CCAGCAAGAT 
AATGTGATTT ATGCAAACGG CCATCGAACC AACGAACACA AGCGTCTGTA CACCACATTC 
AGCATATGTG TGATCTCCAA ATATCCCTAT TATAACGCCC TCAGAGACTG CCTGTCCTGT 
TTTCTGTTCC AGATGAAGAC ATCCCGTGTG TCTGACTTTG ATGAGCAGGT GAAGGAGTTT 
TCTGCCAAAC TGGCGCTGGT GCCCATCCCT CCATCTGGAC CACTGCATGT GGTGTTTAAT 
CTCAGACCGC TTCAGATTGA GCTTCCCTCC AGACTGGATG TGGATCGACC CGTAATGGAC 
CTCGATCTCC ATCTGCCATT TCTGTGCTTT AAACCCAAGC AGATCCTGCA GATCATCAGC 
AGTATCCTGA TGGAGCAGAG AGTGGTGTTT CTGTCCACAG ACTGGGCCAA ACTCACACTG 
GTGTCCGAGT GCTTCATGAT CTTTATTCAC CCTCTGCGCT GGCAGCATCC GTTTGTGCCC 
GTCCTGTCCC GACAGATGCT GGACTTCATC ATGGCGCCCA CGGCCTACCT GATGGGCTGC 
CACACGTACC ACTTCCAGGA GGTGGCAGAG GAGATTGAGG ATCTGGTGCT GATAGACATT 
GACCAGGGAA CAGTGTCGTC TTCAACATCA GACAGATTTG ATCTTCCAGA CGTGCCGCTT 
GCAGCCAGAG ATTGTTTTAT ATTCCGCTGT GGGGGTCTGC AGCTCCACTA TGATCTGGAT 
GTGTGCGGCT CCAGCAGTCA GACTGATGTG AATGATCTGC GAGCTCACAG AAGACTCTGG 
CAGCGCAAAC TCAACTCCAG CATCCTCAAC ATCAGCATGG AGCTCATCGT CAACATATTC 
AGTGAGGTTT GTGATTTCCT GAACTATGAG CATCGAGTTT TCAACAGCGA GGAGTTCCTC 
AGGTCCAGAG ACTCCACCGA CTCTGCCTTT TACAGAAAGG TTTTAGACAC TCATATATTC 
CACTCGTTTC TGCGGGAGCG TTTGAACAGA AAGAATGACG CATTCACTCG CATGCAGCTC 
AACATCCGCT CAGAGACTCG CAGGCTGAAG ATTATGACCG AGTCTCCGCG CAGACCCACG 
ATGGAGGAGA TCAACCGTAA ACACCGAGCA GAGAACGGCC TGAATAAGCG TTTGGGGATG 
AGCATGCCGA ACCTGGCGGA CTCTCGATGT CTCACCGTAC CCATCCGACA GATGTCCATG 
ATGTCAGTGA GGATGCCTTT ACAGATGGGT TCCTGGAGTC CTATAAAGCC AGTCAAAACC 
TTCAAACTCC CAGAATTCCC TCCACCACTG GCCTACCACT ATGTACAGAA CTACTACCAC 
GATCTGATTG GGCAGTTGGA CAGATCCATC TCATCTGCTT CCCCAGACGA CTCTGCTCTC 
TTGGCCCGCT ATTATTACCT GCGTGGACTG GTGAATTCTG TGACTGGACG ACATGTGGAC 
GCCCTCGCAG ACTTCCAGAG CTTATATAAG ACGGACATGG AGATTTTTCC TGCGCAGCTT 
CTGAACACTC TGGTGGAGGC TCTGCCTGTG GAGGAAAAGA GGATTGCGGA GCGCCGCACC 
GATCTGAAGC GTCTCATGAG CAGGGTGAGG AGGGAAAACG AAAAGGACCG AGCGCGGCTG 
GTGGACGGCG GGACGGTGAA GAGGTTTGAG CTGCCCAAAA AACACCTGCA AAAGGAGGAT 
TTTGTGCGGC GCGTTCAGGA GTCGGGAATC GTGAAGGATC TGGAGACCAT CAAGAGGCTG 
TTCGAGGCAC TCACTCTGGG TCAGGTGAAG CAGGTGGATC CAGAAACCTT CCGCTTCTTC 
TACAACTTCT GGAAGGAAAC GGAAGCGGCG GCGCAGGACG TGGATCTGCC GGCCGTCGTG 
TTGGAGCATC TGGAGAACGA TGAGTGTGTT TACAAAGTCT CGTCATCTGT AAAAACCAGC 
CACGGTGTGG GGAAGATCGC CATGACCCAG CGCAGACTCT TCCTGCTGAC GGAGGGACGA 
CCCGGATACG TGGAGATCAC CAAATTCAGA GACATCGAGG ATGTAAGGAT TTCCTCTGCC 
CCGTTCCTCC TCCTGAGAAT CCCATCACTG AAGATCAGGA CGTCCCTCAG GAGGGAGTCA 
TTCGAGGCCA ATCTGAAGTC CGAGTGTGAC CTCTGGCACC TGATGATCAA AGAGATGTGG 
GCCGGGAGGA AGATGGCGGA CCAACATAAG GATCCTCAGT TTATGGAGCA GGCTTTGACT 
AACGCTCTTC TGATGGACGC TGTGGTGGGC AGTCTGCAGT CTCAGAAAGC CATCTACGCT 
GCCACTAAAC TGGCTTACTT TGACAAGATG AAGTCTGAGG TGCCCATGAT GGTCCCCAAA 
ACCACCTCTG ACACCCTGAA GCACAAGATC AACCCGTCTC TGGACCAGAC GAGTCCTCAA 
GCAGTGGACG TTCTGCTCTA CACTCCAGGT CAGCTGGGTG TGTCAGATGT GGACGGTGGT 
GGGAACCCGA AGCTGTGGTG TGCTCTGAGT GATGGGAAGC TGCTAGTTTT TGACGCCGCC 
AGCTGGTCCA TGCAGCACAA CTCTGTTCAA GTCGGGACGT CCCGTCTGAC GTGTATGCTG 
GCAGTGAATG AGCAGCAGCT GTGGATCGGC TCTAAAGATT CCTTCATCTA CATCATAAAC 
CCGCGCAGCG TCTCCTGCAA TAAACAGCTG ACGGAGCATC GCTGTGAGCT CACCGGGCTC 
GCCCTGGAGG AGAGAGCCGA CAAATTCAGC CAGACGGTGG CGTACTCCTG CAGTGAGGAC 
TGCAATGTGA TCGTGTGGGA CGTTTCCTCA CTGCAGGTCA GACGGCAGTT CAGAGTGTCC 
TGCGGCCGAC TGCAGTCCAT GCACGTCCAC AACGGCATGC TGTGGTGCTG TGGTCGTGAT 
TGTGTGCTGG AGGTGCGCAG GAACGGCTCG GTTCACCGTC GGATCTCTCT GCCCGATCAC 
CTGCTGAGCT CACCAAAGGG CTTCAGCAGT TTCCTGCTCT TTAATGAGTC GGAGCAGCTG 
TGGGCTGGTT TTGCGGATGC GGGTGAGCTG TGTGTTTGGC ACTGCAGAGA CATCAGCAAA 
CCCTTCATCA GGATTCAGCT CAAAGACTGC GCCGCCGTCA CCTGCATGAT CAGGGTCAAG 
AACCAGATCT GGGTCGGCGG TTACAATGGC AGCGTCACAG ACAGACGAGT GCGAGGGAAG 
ATCTACATAG TAAACACGGA CCGATATACA GTGGAGAAAG AGCTGCAGGC CCACGCCGAC 
GCCGTTCAGA CCATGTGTTC AGCCGAAGAC CGATATGTGC TCAGCGGAGC CGCACAGGAT 
GATGGGAAAA TCGGCATCTG GAAGGTGGAG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001338419.4
CDS	389..4201
Translation	MADNVPCGLLEACVLLGVSNERLKELCPGKDADVLVEAEVLQVHAPPFITKDRSCNGGVDQGVAPAFSRVQKRRSFKKKKRERPVSTLSNSSQASESEELSVPQNIDLIALPQLCFPDGLRITNESREDSYHFLVFTDVFGNQTHGVVAQYCRPVQFQQDNVIYANGHRTNEHKRLYTTFSICVISKYPYYNALRDCLSCFLFQMKTSRVSDFDEQVKEFSAKLALVPIPPSGPLHVVFNLRPLQIELPSRLDVDRPVMDLDLHLPFLCFKPKQILQIISSILMEQRVVFLSTDWAKLTLVSECFMIFIHPLRWQHPFVPVLSRQMLDFIMAPTAYLMGCHTYHFQEVAEEIEDLVLIDIDQGTVSSSTSDRFDLPDVPLAARDCFIFRCGGLQLHYDLDVCGSSSQTDVNDLRAHRRLWQRKLNSSILNISMELIVNIFSEVCDFLNYEHRVFNSEEFLRSRDSTDSAFYRKVLDTHIFHSFLRERLNRKNDAFTRMQLNIRSETRRLKIMTESPRRPTMEEINRKHRAENGLNKRLGMSMPNLADSRCLTVPIRQMSMMSVRMPLQMGSWSPIKPVKTFKLPEFPPPLAYHYVQNYYHDLIGQLDRSISSASPDDSALLARYYYLRGLVNSVTGRHVDALADFQSLYKTDMEIFPAQLLNTLVEALPVEEKRIAERRTDLKRLMSRVRRENEKDRARLVDGGTVKRFELPKKHLQKEDFVRRVQESGIVKDLETIKRLFEALTLGQVKQVDPETFRFFYNFWKETEAAAQDVDLPAVVLEHLENDECVYKVSSSVKTSHGVGKIAMTQRRLFLLTEGRPGYVEITKFRDIEDVRISSAPFLLLRIPSLKIRTSLRRESFEANLKSECDLWHLMIKEMWAGRKMADQHKDPQFMEQALTNALLMDAVVGSLQSQKAIYAATKLAYFDKMKSEVPMMVPKTTSDTLKHKINPSLDQTSPQAVDVLLYTPGQLGVSDVDGGGNPKLWCALSDGKLLVFDAASWSMQHNSVQVGTSRLTCMLAVNEQQLWIGSKDSFIYIINPRSVSCNKQLTEHRCELTGLALEERADKFSQTVAYSCSEDCNVIVWDVSSLQVRRQFRVSCGRLQSMHVHNGMLWCCGRDCVLEVRRNGSVHRRISLPDHLLSSPKGFSSFLLFNESEQLWAGFADAGELCVWHCRDISKPFIRIQLKDCAAVTCMIRVKNQIWVGGYNGSVTDRRVRGKIYIVNTDRYTVEKELQAHADAVQTMCSAEDRYVLSGAAQDDGKIGIWKVE

Target ORF information:

RefSeq Version	XM_001338383.6
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio DENN/MADD domain containing 3a (dennd3a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001338383.6

ORF Insert Sequence:

ATGGCTGATA ATGTGCCGTG TGGTCTGCTG GAGGCCTGTG TGCTGCTTGG AGTGTCAAAC 
GAGCGCCTGA AGGAGCTGTG CCCGGGTAAA GATGCAGATG TGTTGGTGGA GGCGGAGGTG 
CTGCAGGTTC ACGCTCCACC GTTTATAACA AAGGATCGCT CTTGTAACGG CGGCGTAGAC 
CAGGGCGTGG CTCCGGCCTT TAGCCGAGTC CAGAAGAGAC GCTCCTTCAA GAAGAAGAAG 
AGAGAGCGGC CGGTGTCAAC CCTCAGCAAC TCCAGTCAAG CCAGTGAATC TGAAGAGCTG 
AGCGTCCCGC AGAACATTGA TCTGATCGCC CTGCCACAGC TCTGCTTTCC TGATGGGTTG 
AGAATCACCA ATGAGAGCAG AGAAGACTCG TATCACTTCC TGGTGTTCAC GGATGTGTTC 
GGGAATCAGA CTCATGGTGT GGTCGCTCAG TACTGCAGAC CTGTACAGTT CCAGCAAGAT 
AATGTGATTT ATGCAAACGG CCATCGAACC AACGAACACA AGCGTCTGTA CACCACATTC 
AGCATATGTG TGATCTCCAA ATATCCCTAT TATAACGCCC TCAGAGACTG CCTGTCCTGT 
TTTCTGTTCC AGATGAAGAC ATCCCGTGTG TCTGACTTTG ATGAGCAGGT GAAGGAGTTT 
TCTGCCAAAC TGGCGCTGGT GCCCATCCCT CCATCTGGAC CACTGCATGT GGTGTTTAAT 
CTCAGACCGC TTCAGATTGA GCTTCCCTCC AGACTGGATG TGGATCGACC CGTAATGGAC 
CTCGATCTCC ATCTGCCATT TCTGTGCTTT AAACCCAAGC AGATCCTGCA GATCATCAGC 
AGTATCCTGA TGGAGCAGAG AGTGGTGTTT CTGTCCACAG ACTGGGCCAA ACTCACACTG 
GTGTCCGAGT GCTTCATGAT CTTTATTCAC CCTCTGCGCT GGCAGCATCC GTTTGTGCCC 
GTCCTGTCCC GACAGATGCT GGACTTCATC ATGGCGCCCA CGGCCTACCT GATGGGCTGC 
CACACGTACC ACTTCCAGGA GGTGGCAGAG GAGATTGAGG ATCTGGTGCT GATAGACATT 
GACCAGGGAA CAGTGTCGTC TTCAACATCA GACAGATTTG ATCTTCCAGA CGTGCCGCTT 
GCAGCCAGAG ATTGTTTTAT ATTCCGCTGT GGGGGTCTGC AGCTCCACTA TGATCTGGAT 
GTGTGCGGCT CCAGCAGTCA GACTGATGTG AATGATCTGC GAGCTCACAG AAGACTCTGG 
CAGCGCAAAC TCAACTCCAG CATCCTCAAC ATCAGCATGG AGCTCATCGT CAACATATTC 
AGTGAGGTTT GTGATTTCCT GAACTATGAG CATCGAGTTT TCAACAGCGA GGAGTTCCTC 
AGGTCCAGAG ACTCCACCGA CTCTGCCTTT TACAGAAAGG TTTTAGACAC TCATATATTC 
CACTCGTTTC TGCGGGAGCG TTTGAACAGA AAGAATGACG CATTCACTCG CATGCAGCTC 
AACATCCGCT CAGAGACTCG CAGGCTGAAG ATTATGACCG AGTCTCCGCG CAGACCCACG 
ATGGAGGAGA TCAACCGTAA ACACCGAGCA GAGAACGGCC TGAATAAGCG TTTGGGGATG 
AGCATGCCGA ACCTGGCGGA CTCTCGATGT CTCACCGTAC CCATCCGACA GATGTCCATG 
ATGTCAGTGA GGATGCCTTT ACAGATGGGT TCCTGGAGTC CTATAAAGCC AGTCAAAACC 
TTCAAACTCC CAGAATTCCC TCCACCACTG GCCTACCACT ATGTACAGAA CTACTACCAC 
GATCTGATTG GGCAGTTGGA CAGATCCATC TCATCTGCTT CCCCAGACGA CTCTGCTCTC 
TTGGCCCGCT ATTATTACCT GCGTGGACTG GTGAATTCTG TGACTGGACG ACATGTGGAC 
GCCCTCGCAG ACTTCCAGAG CTTATATAAG ACGGACATGG AGATTTTTCC TGCGCAGCTT 
CTGAACACTC TGGTGGAGGC TCTGCCTGTG GAGGAAAAGA GGATTGCGGA GCGCCGCACC 
GATCTGAAGC GTCTCATGAG CAGGGTGAGG AGGGAAAACG AAAAGGACCG AGCGCGGCTG 
GTGGACGGCG GGACGGTGAA GAGGTTTGAG CTGCCCAAAA AACACCTGCA AAAGGAGGAT 
TTTGTGCGGC GCGTTCAGGA GTCGGGAATC GTGAAGGATC TGGAGACCAT CAAGAGGCTG 
TTCGAGGCAC TCACTCTGGG TCAGGTGAAG CAGGTGGATC CAGAAACCTT CCGCTTCTTC 
TACAACTTCT GGAAGGAAAC GGAAGCGGCG GCGCAGGACG TGGATCTGCC GGCCGTCGTG 
TTGGAGCATC TGGAGAACGA TGAGTGTGTT TACAAAGTCT CGTCATCTGT AAAAACCAGC 
CACGGTGTGG GGAAGATCGC CATGACCCAG CGCAGACTCT TCCTGCTGAC GGAGGGACGA 
CCCGGATACG TGGAGATCAC CAAATTCAGA GACATCGAGG ATGTAAGGAT TTCCTCTGCC 
CCGTTCCTCC TCCTGAGAAT CCCATCACTG AAGATCAGGA CGTCCCTCAG GAGGGAGTCA 
TTCGAGGCCA ATCTGAAGTC CGAGTGTGAC CTCTGGCACC TGATGATCAA AGAGATGTGG 
GCCGGGAGGA AGATGGCGGA CCAACATAAG GATCCTCAGT TTATGGAGCA GGCTTTGACT 
AACGCTCTTC TGATGGACGC TGTGGTGGGC AGTCTGCAGT CTCAGAAAGC CATCTACGCT 
GCCACTAAAC TGGCTTACTT TGACAAGATG AAGTCTGAGG TGCCCATGAT GGTCCCCAAA 
ACCACCTCTG ACACCCTGAA GCACAAGATC AACCCGTCTC TGGACCAGAC GAGTCCTCAA 
GCAGTGGACG TTCTGCTCTA CACTCCAGGT CAGCTGGGTG TGTCAGATGT GGACGGTGGT 
GGGAACCCGA AGCTGTGGTG TGCTCTGAGT GATGGGAAGC TGCTAGTTTT TGACGCCGCC 
AGCTGGTCCA TGCAGCACAA CTCTGTTCAA GTCGGGACGT CCCGTCTGAC GTGTATGCTG 
GCAGTGAATG AGCAGCAGCT GTGGATCGGC TCTAAAGATT CCTTCATCTA CATCATAAAC 
CCGCGCAGCG TCTCCTGCAA TAAACAGCTG ACGGAGCATC GCTGTGAGCT CACCGGGCTC 
GCCCTGGAGG AGAGAGCCGA CAAATTCAGC CAGACGGTGG CGTACTCCTG CAGTGAGGAC 
TGCAATGTGA TCGTGTGGGA CGTTTCCTCA CTGCAGGTCA GACGGCAGTT CAGAGTGTCC 
TGCGGCCGAC TGCAGTCCAT GCACGTCCAC AACGGCATGC TGTGGTGCTG TGGTCGTGAT 
TGTGTGCTGG AGGTGCGCAG GAACGGCTCG GTTCACCGTC GGATCTCTCT GCCCGATCAC 
CTGCTGAGCT CACCAAAGGG CTTCAGCAGT TTCCTGCTCT TTAATGAGTC GGAGCAGCTG 
TGGGCTGGTT TTGCGGATGC GGGTGAGCTG TGTGTTTGGC ACTGCAGAGA CATCAGCAAA 
CCCTTCATCA GGATTCAGCT CAAAGACTGC GCCGCCGTCA CCTGCATGAT CAGGGTCAAG 
AACCAGATCT GGGTCGGCGG TTACAATGGC AGCGTCACAG ACAGACGAGT GCGAGGGAAG 
ATCTACATAG TAAACACGGA CCGATATACA GTGGAGAAAG AGCTGCAGGC CCACGCCGAC 
GCCGTTCAGA CCATGTGTTC AGCCGAAGAC CGATATGTGC TCAGCGGAGC CGCACAGGAT 
GATGGGAAAA TCGGCATCTG GAAGGTGGAG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DENND3A ( XM_009293932.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009292207.1 Danio rerio DENN/MADD domain containing 3a (dennd3a), transcript variant X3, mRNA.

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CloneID	ODa47230
Clone ID Related Accession (Same CDS sequence)	XM_009293932.2
Accession Version	XM_009293932.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3246bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	DENN domain-containing protein 3 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007130.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009293932.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGATA ATGTGCCGTG TGGTCTGCTG GAGGCCTGTG TGCTGCTTGG AGTGTCAAAC 
GAGCGCCTGA AGGAGCTGTG CCCGGGTAAA GATGCAGATG TGTTGGTGGA GGCGGAGGTG 
CTGCAGGTTC ACGCTCCACC GTTTATAACA AAGGATCGCT CTTGTAACGG CGGCGTAGAC 
CAGGGCGTGG CTCCGGCCTT TAGCCGAGTC CAGAAGAGAC GCTCCTTCAA GAAGAAGAAG 
AGAGAGCGGC CGGTGTCAAC CCTCAGCAAC TCCAGTCAAG CCAGTGAATC TGAAGAGCTG 
AGCGTCCCGC AGAACATTGA TCTGATCGCC CTGCCACAGC TCTGCTTTCC TGATGGGTTG 
AGAATCACCA ATGAGAGCAG AGAAGACTCG TATCACTTCC TGGTGTTCAC GGATGTGTTC 
GGGAATCAGA CTCATGGTGT GGTCGCTCAG TACTGCAGAC CTGTACAGTT CCAGCAAGAT 
AATGTGATTT ATGCAAACGG CCATCGAACC AACGAACACA AGCGTCTGTA CACCACATTC 
AGCATATGTG TGATCTCCAA ATATCCCTAT TATAACGCCC TCAGAGACTG CCTGTCCTGT 
TTTCTGTTCC AGATGAAGAC ATCCCGTGTG TCTGACTTTG ATGAGCAGGT GAAGGAGTTT 
TCTGCCAAAC TGGCGCTGGT GCCCATCCCT CCATCTGGAC CACTGCATGT GGTGTTTAAT 
CTCAGACCGC TTCAGATTGA GCTTCCCTCC AGACTGGATG TGGATCGACC CGTAATGGAC 
CTCGATCTCC ATCTGCCATT TCTGTGCTTT AAACCCAAGC AGATCCTGCA GATCATCAGC 
AGTATCCTGA TGGAGCAGAG AGTGGTGTTT CTGTCCACAG ACTGGGCCAA ACTCACACTG 
GTGTCCGAGT GCTTCATGAT CTTTATTCAC CCTCTGCGCT GGCAGCATCC GTTTGTGCCC 
GTCCTGTCCC GACAGATGCT GGACTTCATC ATGGCGCCCA CGGCCTACCT GATGGGCTGC 
CACACGTACC ACTTCCAGGA GGTGGCAGAG GAGATTGAGG ATCTGGTGCT GATAGACATT 
GACCAGGGAA CAGTGTCGTC TTCAACATCA GACAGATTTG ATCTTCCAGA CGTGCCGCTT 
GCAGCCAGAG ATTGTTTTAT ATTCCGCTGT GGGGGTCTGC AGCTCCACTA TGATCTGGAT 
GTGTGCGGCT CCAGCAGTCA GACTGATGTG AATGATCTGC GAGCTCACAG AAGACTCTGG 
CAGCGCAAAC TCAACTCCAG CATCCTCAAC ATCAGCATGG AGCTCATCGT CAACATATTC 
AGTGAGGTTT GTGATTTCCT GAACTATGAG CATCGAGTTT TCAACAGCGA GGAGTTCCTC 
AGGTCCAGAG ACTCCACCGA CTCTGCCTTT TACAGAAAGG TTTTAGACAC TCATATATTC 
CACTCGTTTC TGCGGGAGCG TTTGAACAGA AAGAATGACG CATTCACTCG CATGCAGCTC 
AACATCCGCT CAGAGACTCG CAGGCTGAAG ATTATGACCG AGTCTCCGCG CAGACCCACG 
ATGGAGGAGA TCAACCGTAA ACACCGAGCA GAGAACGGCC TGAATAAGCG TTTGGGGATG 
AGCATGCCGA ACCTGGCGGA CTCTCGATGT CTCACCGTAC CCATCCGACA GATGTCCATG 
ATGTCAGTGA GGATGCCTTT ACAGATGGGT TCCTGGAGTC CTATAAAGCC AGTCAAAACC 
TTCAAACTCC CAGAATTCCC TCCACCACTG GCCTACCACT ATGTACAGAA CTACTACCAC 
GATCTGATTG GGCAGTTGGA CAGATCCATC TCATCTGCTT CCCCAGACGA CTCTGCTCTC 
TTGGCCCGCT ATTATTACCT GCGTGGACTG GTGAATTCTG TGACTGGACG ACATGTGGAC 
GCCCTCGCAG ACTTCCAGAG CTTATATAAG ACGGACATGG AGATTTTTCC TGCGCAGCTT 
CTGAACACTC TGGTGGAGGC TCTGCCTGTG GAGGAAAAGA GGATTGCGGA GCGCCGCACC 
GATCTGAAGC GTCTCATGAG CAGGGTGAGG AGGGAAAACG AAAAGGACCG AGCGCGGCTG 
GTGGACGGCG GGACGGTGAA GAGGTTTGAG CTGCCCAAAA AACACCTGCA AAAGGAGGAT 
TTTGTGCGGC GCGTTCAGGA GTCGGGAATC GTGAAGGATC TGGAGACCAT CAAGAGGCTG 
TTCGAGGCAC TCACTCTGGG TCAGGTGAAG CAGGTGGATC CAGAAACCTT CCGCTTCTTC 
TACAACTTCT GGAAGGAAAC GGAAGCGGCG GCGCAGGACG TGGATCTGCC GGCCGTCGTG 
TTGGAGCATC TGGAGAACGA TGAGTGTGTT TACAAAGTCT CGTCATCTGT AAAAACCAGC 
CACGGTGTGG GGAAGATCGC CATGACCCAG CGCAGACTCT TCCTGCTGAC GGAGGGACGA 
CCCGGATACG TGGAGATCAC CAAATTCAGA GACATCGAGG ATGTAAGGAT TTCCTCTGCC 
CCGTTCCTCC TCCTGAGAAT CCCATCACTG AAGATCAGGA CGTCCCTCAG GAGGGAGTCA 
TTCGAGGCCA ATCTGAAGTC CGAGTGTGAC CTCTGGCACC TGATGATCAA AGAGATGTGG 
GCCGGGAGGA AGATGGCGGA CCAACATAAG GATCCTCAGT TTATGGAGCA GGCTTTGACT 
AACGCTCTTC TGATGGACGC TGTGGTGGGC AGTCTGCAGT CTCAGAAAGC CATCTACGCT 
GCCACTAAAC TGGCTTACTT TGACAAGATG AAGTCTGAGG TGCCCATGAT GGTCCCCAAA 
ACCACCTCTG ACACCCTGAA GCACAAGATC AACCCGTCTC TGGACCAGAC GAGTCCTCAA 
GCAGTGGACG TTCTGCTCTA CACTCCAGGT CAGCTGGGTG TGTCAGATGT GGACGGTGGT 
GGGAACCCGA AGCTGTGGTG TGCTCTGAGT GATGGGAAGC TGCTAGTTTT TGACGCCGCC 
AGCTGGTCCA TGCAGCACAA CTCTGTTCAA GTCGGGACGT CCCGTCTGAC GTGTATGCTG 
GCAGTGAATG AGCAGCAGCT GTGGATCGGC TCTAAAGATT CCTTCATCTA CATCATAAAC 
CCGCGCAGCG TCTCCTGCAA TAAACAGCTG ACGGAGCATC GCTGTGAGCT CACCGGGCTC 
GCCCTGGAGG AGAGAGCCGA CAAATTCAGA CGGTGGCGTA CTCCTGCAGT GAGGACTGCA 
ATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009292207.1
CDS	394..3639
Translation	MADNVPCGLLEACVLLGVSNERLKELCPGKDADVLVEAEVLQVHAPPFITKDRSCNGGVDQGVAPAFSRVQKRRSFKKKKRERPVSTLSNSSQASESEELSVPQNIDLIALPQLCFPDGLRITNESREDSYHFLVFTDVFGNQTHGVVAQYCRPVQFQQDNVIYANGHRTNEHKRLYTTFSICVISKYPYYNALRDCLSCFLFQMKTSRVSDFDEQVKEFSAKLALVPIPPSGPLHVVFNLRPLQIELPSRLDVDRPVMDLDLHLPFLCFKPKQILQIISSILMEQRVVFLSTDWAKLTLVSECFMIFIHPLRWQHPFVPVLSRQMLDFIMAPTAYLMGCHTYHFQEVAEEIEDLVLIDIDQGTVSSSTSDRFDLPDVPLAARDCFIFRCGGLQLHYDLDVCGSSSQTDVNDLRAHRRLWQRKLNSSILNISMELIVNIFSEVCDFLNYEHRVFNSEEFLRSRDSTDSAFYRKVLDTHIFHSFLRERLNRKNDAFTRMQLNIRSETRRLKIMTESPRRPTMEEINRKHRAENGLNKRLGMSMPNLADSRCLTVPIRQMSMMSVRMPLQMGSWSPIKPVKTFKLPEFPPPLAYHYVQNYYHDLIGQLDRSISSASPDDSALLARYYYLRGLVNSVTGRHVDALADFQSLYKTDMEIFPAQLLNTLVEALPVEEKRIAERRTDLKRLMSRVRRENEKDRARLVDGGTVKRFELPKKHLQKEDFVRRVQESGIVKDLETIKRLFEALTLGQVKQVDPETFRFFYNFWKETEAAAQDVDLPAVVLEHLENDECVYKVSSSVKTSHGVGKIAMTQRRLFLLTEGRPGYVEITKFRDIEDVRISSAPFLLLRIPSLKIRTSLRRESFEANLKSECDLWHLMIKEMWAGRKMADQHKDPQFMEQALTNALLMDAVVGSLQSQKAIYAATKLAYFDKMKSEVPMMVPKTTSDTLKHKINPSLDQTSPQAVDVLLYTPGQLGVSDVDGGGNPKLWCALSDGKLLVFDAASWSMQHNSVQVGTSRLTCMLAVNEQQLWIGSKDSFIYIINPRSVSCNKQLTEHRCELTGLALEERADKFRRWRTPAVRTAM

Target ORF information:

RefSeq Version	XM_009293932.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio DENN/MADD domain containing 3a (dennd3a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009293932.2

ORF Insert Sequence:

ATGGCTGATA ATGTGCCGTG TGGTCTGCTG GAGGCCTGTG TGCTGCTTGG AGTGTCAAAC 
GAGCGCCTGA AGGAGCTGTG CCCGGGTAAA GATGCAGATG TGTTGGTGGA GGCGGAGGTG 
CTGCAGGTTC ACGCTCCACC GTTTATAACA AAGGATCGCT CTTGTAACGG CGGCGTAGAC 
CAGGGCGTGG CTCCGGCCTT TAGCCGAGTC CAGAAGAGAC GCTCCTTCAA GAAGAAGAAG 
AGAGAGCGGC CGGTGTCAAC CCTCAGCAAC TCCAGTCAAG CCAGTGAATC TGAAGAGCTG 
AGCGTCCCGC AGAACATTGA TCTGATCGCC CTGCCACAGC TCTGCTTTCC TGATGGGTTG 
AGAATCACCA ATGAGAGCAG AGAAGACTCG TATCACTTCC TGGTGTTCAC GGATGTGTTC 
GGGAATCAGA CTCATGGTGT GGTCGCTCAG TACTGCAGAC CTGTACAGTT CCAGCAAGAT 
AATGTGATTT ATGCAAACGG CCATCGAACC AACGAACACA AGCGTCTGTA CACCACATTC 
AGCATATGTG TGATCTCCAA ATATCCCTAT TATAACGCCC TCAGAGACTG CCTGTCCTGT 
TTTCTGTTCC AGATGAAGAC ATCCCGTGTG TCTGACTTTG ATGAGCAGGT GAAGGAGTTT 
TCTGCCAAAC TGGCGCTGGT GCCCATCCCT CCATCTGGAC CACTGCATGT GGTGTTTAAT 
CTCAGACCGC TTCAGATTGA GCTTCCCTCC AGACTGGATG TGGATCGACC CGTAATGGAC 
CTCGATCTCC ATCTGCCATT TCTGTGCTTT AAACCCAAGC AGATCCTGCA GATCATCAGC 
AGTATCCTGA TGGAGCAGAG AGTGGTGTTT CTGTCCACAG ACTGGGCCAA ACTCACACTG 
GTGTCCGAGT GCTTCATGAT CTTTATTCAC CCTCTGCGCT GGCAGCATCC GTTTGTGCCC 
GTCCTGTCCC GACAGATGCT GGACTTCATC ATGGCGCCCA CGGCCTACCT GATGGGCTGC 
CACACGTACC ACTTCCAGGA GGTGGCAGAG GAGATTGAGG ATCTGGTGCT GATAGACATT 
GACCAGGGAA CAGTGTCGTC TTCAACATCA GACAGATTTG ATCTTCCAGA CGTGCCGCTT 
GCAGCCAGAG ATTGTTTTAT ATTCCGCTGT GGGGGTCTGC AGCTCCACTA TGATCTGGAT 
GTGTGCGGCT CCAGCAGTCA GACTGATGTG AATGATCTGC GAGCTCACAG AAGACTCTGG 
CAGCGCAAAC TCAACTCCAG CATCCTCAAC ATCAGCATGG AGCTCATCGT CAACATATTC 
AGTGAGGTTT GTGATTTCCT GAACTATGAG CATCGAGTTT TCAACAGCGA GGAGTTCCTC 
AGGTCCAGAG ACTCCACCGA CTCTGCCTTT TACAGAAAGG TTTTAGACAC TCATATATTC 
CACTCGTTTC TGCGGGAGCG TTTGAACAGA AAGAATGACG CATTCACTCG CATGCAGCTC 
AACATCCGCT CAGAGACTCG CAGGCTGAAG ATTATGACCG AGTCTCCGCG CAGACCCACG 
ATGGAGGAGA TCAACCGTAA ACACCGAGCA GAGAACGGCC TGAATAAGCG TTTGGGGATG 
AGCATGCCGA ACCTGGCGGA CTCTCGATGT CTCACCGTAC CCATCCGACA GATGTCCATG 
ATGTCAGTGA GGATGCCTTT ACAGATGGGT TCCTGGAGTC CTATAAAGCC AGTCAAAACC 
TTCAAACTCC CAGAATTCCC TCCACCACTG GCCTACCACT ATGTACAGAA CTACTACCAC 
GATCTGATTG GGCAGTTGGA CAGATCCATC TCATCTGCTT CCCCAGACGA CTCTGCTCTC 
TTGGCCCGCT ATTATTACCT GCGTGGACTG GTGAATTCTG TGACTGGACG ACATGTGGAC 
GCCCTCGCAG ACTTCCAGAG CTTATATAAG ACGGACATGG AGATTTTTCC TGCGCAGCTT 
CTGAACACTC TGGTGGAGGC TCTGCCTGTG GAGGAAAAGA GGATTGCGGA GCGCCGCACC 
GATCTGAAGC GTCTCATGAG CAGGGTGAGG AGGGAAAACG AAAAGGACCG AGCGCGGCTG 
GTGGACGGCG GGACGGTGAA GAGGTTTGAG CTGCCCAAAA AACACCTGCA AAAGGAGGAT 
TTTGTGCGGC GCGTTCAGGA GTCGGGAATC GTGAAGGATC TGGAGACCAT CAAGAGGCTG 
TTCGAGGCAC TCACTCTGGG TCAGGTGAAG CAGGTGGATC CAGAAACCTT CCGCTTCTTC 
TACAACTTCT GGAAGGAAAC GGAAGCGGCG GCGCAGGACG TGGATCTGCC GGCCGTCGTG 
TTGGAGCATC TGGAGAACGA TGAGTGTGTT TACAAAGTCT CGTCATCTGT AAAAACCAGC 
CACGGTGTGG GGAAGATCGC CATGACCCAG CGCAGACTCT TCCTGCTGAC GGAGGGACGA 
CCCGGATACG TGGAGATCAC CAAATTCAGA GACATCGAGG ATGTAAGGAT TTCCTCTGCC 
CCGTTCCTCC TCCTGAGAAT CCCATCACTG AAGATCAGGA CGTCCCTCAG GAGGGAGTCA 
TTCGAGGCCA ATCTGAAGTC CGAGTGTGAC CTCTGGCACC TGATGATCAA AGAGATGTGG 
GCCGGGAGGA AGATGGCGGA CCAACATAAG GATCCTCAGT TTATGGAGCA GGCTTTGACT 
AACGCTCTTC TGATGGACGC TGTGGTGGGC AGTCTGCAGT CTCAGAAAGC CATCTACGCT 
GCCACTAAAC TGGCTTACTT TGACAAGATG AAGTCTGAGG TGCCCATGAT GGTCCCCAAA 
ACCACCTCTG ACACCCTGAA GCACAAGATC AACCCGTCTC TGGACCAGAC GAGTCCTCAA 
GCAGTGGACG TTCTGCTCTA CACTCCAGGT CAGCTGGGTG TGTCAGATGT GGACGGTGGT 
GGGAACCCGA AGCTGTGGTG TGCTCTGAGT GATGGGAAGC TGCTAGTTTT TGACGCCGCC 
AGCTGGTCCA TGCAGCACAA CTCTGTTCAA GTCGGGACGT CCCGTCTGAC GTGTATGCTG 
GCAGTGAATG AGCAGCAGCT GTGGATCGGC TCTAAAGATT CCTTCATCTA CATCATAAAC 
CCGCGCAGCG TCTCCTGCAA TAAACAGCTG ACGGAGCATC GCTGTGAGCT CACCGGGCTC 
GCCCTGGAGG AGAGAGCCGA CAAATTCAGA CGGTGGCGTA CTCCTGCAGT GAGGACTGCA 
ATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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DENND3A cDNA ORF clone, Danio rerio(Zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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FAX

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