gRNAs
The following SCUBE1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SCUBE1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
| CloneID | ODa47222 | |
| Clone ID Related Accession (Same CDS sequence) | XM_009300177.2 | |
| Accession Version | XM_009300177.2 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 2301bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector |  User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2016-06-23 | |
| Organism | Danio rerio(Zebrafish) | |
| Product | signal peptide, CUB and EGF-like domain-containing protein 1 isoform X1 | |
| Comment | MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009300177.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGCCCTGCA TTCAGACAGC AAGACCTGCA TTGACCTGTG CGGTGAACAA CGGCGGCTGC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_009298452.1 |
| CDS | 43..2343 |
| Translation |
Target ORF information:
Target ORF information:
|
 XM_009300177.2 |
1 | ATGCCCTGCA TTCAGACAGC AAGACCTGCA TTGACCTGTG CGGTGAACAA CGGCGGCTGC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | ODa60597 | |
| Clone ID Related Accession (Same CDS sequence) | NM_001260500.2 | |
| Accession Version | NM_001260500.2 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 2985bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2018-11-23 | |
| Organism | Danio rerio(zebrafish) | |
| Product | signal peptide, CUB and EGF-like domain-containing protein 1 isoform 2 precursor | |
| Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CT573393.17 and FQ311921.4. On Jun 18, 2016 this sequence version replaced NM_001260500.1. Transcript Variant: This variant (2) lacks an alternate in-frame exon compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: JF900367.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. | |
1 | ATGGGTCCTG CCTGCTTCTC CTGGGAAGTC TGTTTGCTTT TCCTCCTCAT AAATACTTTA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_001247429.2 |
| CDS | 123..3107 |
| Translation |
Target ORF information:
Target ORF information:
|
NM_001260500.2 |
1 | ATGGGTCCTG CCTGCTTCTC CTGGGAAGTC TGTTTGCTTT TCCTCCTCAT AAATACTTTA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | ODa47221 | |
| Clone ID Related Accession (Same CDS sequence) | NM_001328599.1 | |
| Accession Version | NM_001328599.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 3075bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2018-11-23 | |
| Organism | Danio rerio(zebrafish) | |
| Product | signal peptide, CUB and EGF-like domain-containing protein 1 isoform 1 precursor | |
| Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CT573393.17 and FQ311921.4. On Jun 18, 2016 this sequence version replaced XM_009300176.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: JQ740109.1, GFIL01034633.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. | |
1 | ATGGGTCCTG CCTGCTTCTC CTGGGAAGTC TGTTTGCTTT TCCTCCTCAT AAATACTTTA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_001315528.1 |
| CDS | 123..3197 |
| Translation |
Target ORF information:
Target ORF information:
|
NM_001328599.1 |
1 | ATGGGTCCTG CCTGCTTCTC CTGGGAAGTC TGTTTGCTTT TCCTCCTCAT AAATACTTTA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database. |
Electrostatics and N-glycan-mediated membrane tethering of SCUBE1 is critical for promoting bone morphogenetic protein signalling. |
RFX transcription factors are essential for hearing in mice. |
Zebrafish scube1 (signal peptide-CUB (complement protein C1r/C1s, Uegf, and Bmp1)-EGF (epidermal growth factor) domain-containing protein 1) is involved in primitive hematopoiesis. |
Scube activity is necessary for Hedgehog signal transduction in vivo. |
