crfb4 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	crfb4
Entrez Gene ID	797527
Full Name	cytokine receptor family member b4
General protein information	Preferred Names cytokine receptor family member b4 Names interleukin-10 receptor subunit beta il10r2
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001083868.1	NP_001077337.1	interleukin-10 receptor subunit beta precursor

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Il10rb	NP_032375.2
Rattus norvegicus (Norway rat)	Il10rb	NP_001100581.1
Xenopus tropicalis (tropical clawed frog)	il10rb	NP_001165294.1
Homo sapiens (human)	IL10RB	NP_000619.3
Bos taurus (cattle)	IL10RB	NP_001070443.1
Gallus gallus (chicken)	IL10RB	NP_990188.1
Danio rerio (zebrafish)	crfb4	NP_001077337.1
Pan troglodytes (chimpanzee)	IL10RB	XP_531433.2
Canis lupus familiaris (dog)	LOC100856489	XP_003640120.1
	IL10RB	XP_001094340.2
Canis lupus familiaris (dog)	IL10RB	XP_535581.2

Gene Ontology
Bibliography

Related articles in PubMed

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Cloning, identification of the two cytokine receptor family B subunits CRFB1 and CRFB5 from grass carp (Ctenopharyngodon idella).
Chen H, Liu W, Wang B, Mao H, Sun Z, Hou Q, Mi Y, Fan L, Hu C
Fish & shellfish immunology45(2)211-20(2015 Aug)

Atlantic salmon possesses two clusters of type I interferon receptor genes on different chromosomes, which allows for a larger repertoire of interferon receptors than in zebrafish and mammals.
Sun B, Greiner-Tollersrud L, Koop BF, Robertsen B
Developmental and comparative immunology47(2)275-86(2014 Dec)

In vivo protein trapping produces a functional expression codex of the vertebrate proteome.
Clark KJ, Balciunas D, Pogoda HM, Ding Y, Westcot SE, Bedell VM, Greenwood TM, Urban MD, Skuster KJ, Petzold AM, Ni J, Nielsen AL, Patowary A, Scaria V, Sivasubbu S, Xu X, Hammerschmidt M, Ekker SC
Nature methods8(6)506-15(2011 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following crfb4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the crfb4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa35311	NM_001083868.1 Latest version!	Danio rerio cytokine receptor family member b4 (crfb4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

crfb4 ( NM_001083868.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001077337.1 Danio rerio cytokine receptor family member b4 (crfb4), mRNA.

Price & Availability↑

CloneID	ODa35311
Clone ID Related Accession (Same CDS sequence)	NM_001083868.1
Accession Version	NM_001083868.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 942bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	interleukin-10 receptor subunit beta precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from EF014954.1. On Apr 5, 2007 this sequence version replaced XM_001337960.1. ##Evidence-Data-START## Transcript exon combination :: EF014954.1, BC167449.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA4476770, SAMEA4476783 [ECO:0000348] ##Evidence-Data-END##

ATGTCCGCTT TTATCAGTTT TTCTGTTGTC TTGTTTAATA TCATCGGTGT CATCACAGTG 
TTGTCAGAAA ATGTGCCAGT CCCGGAGAAT GTGAGAGTAA TCTCTATAAA CCTGGGATTA 
GTTTTGGAGT GGGATCCACC ACAAACAACT AATAATCATC CATTTAAGTA TACTGCTGAA 
CTGAAAGGGT GGAGGGGTTA TGATCCAGTT TGTCAAAACA GCTCATCGCT AACCTGTGAC 
TTTACTGACA ATGTTACCAT TTTTGGTTCT TACCAACTTC GTGTTCGGAC AGAACTAGAT 
GGAGAAACAT CTGATTGGGT GGAGACTAAA CAAGTTGCAG TGGACAAAAT AACTGAGATT 
AGTGCTCCTC ATGTAGAACT GAGAAGTAAC AATGGACAAA CAGAAGTTGA TATCACTGAT 
CCACCGATGA AAAGAAAAAA CCTGAGAGAC GTCTTTGGGA ATATTTCTTA CCTCATACGT 
TTTTGGAAAA AAGGAGAATC AAATAAGGTC AAACTAAAAA GAGAACAGAG CAGAGTCATG 
TTACCAAAAG TGAAGCCTCT GGAAAACTAC TGTGTCGAAG TAGAGGTTTT GTATTTGAAG 
AATAAAACAA GCCAGCCCAG CAACATGACC TGTTTAAAAA ATTCGCCCAG CAATGAGACA 
GAACCCTGGC TTATAGCTGT GATGCTTTTG ATCAGTTTTC TAGTGGTGTT AGTTGCGGTT 
CTCCTGATCT TTATGGCTGT GTGGTACAGT TACAAAGGAT ACAGAATTGT ATTCCCTAAT 
GATAATCTGC CAGAGCATCT AAAACAGTAT TTGGCACAAC GGTCCCAGTC ACCTGCACCT 
TTACCCATAC ATGAGGGAAC TCACCTGAAG GAGCCCATTC ATGAGTTAAT GATATATGAA 
AAGAAACTTA ACAGCTCTCA GGACAATCAG CAAATAATAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001077337.1
CDS	64..1005
Translation	MSAFISFSVVLFNIIGVITVLSENVPVPENVRVISINLGLVLEWDPPQTTNNHPFKYTAELKGWRGYDPVCQNSSSLTCDFTDNVTIFGSYQLRVRTELDGETSDWVETKQVAVDKITEISAPHVELRSNNGQTEVDITDPPMKRKNLRDVFGNISYLIRFWKKGESNKVKLKREQSRVMLPKVKPLENYCVEVEVLYLKNKTSQPSNMTCLKNSPSNETEPWLIAVMLLISFLVVLVAVLLIFMAVWYSYKGYRIVFPNDNLPEHLKQYLAQRSQSPAPLPIHEGTHLKEPIHELMIYEKKLNSSQDNQQII

Target ORF information:

RefSeq Version	NM_001083868.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio cytokine receptor family member b4 (crfb4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001083868.1

ORF Insert Sequence:

ATGTCCGCTT TTATCAGTTT TTCTGTTGTC TTGTTTAATA TCATCGGTGT CATCACAGTG 
TTGTCAGAAA ATGTGCCAGT CCCGGAGAAT GTGAGAGTAA TCTCTATAAA CCTGGGATTA 
GTTTTGGAGT GGGATCCACC ACAAACAACT AATAATCATC CATTTAAGTA TACTGCTGAA 
CTGAAAGGGT GGAGGGGTTA TGATCCAGTT TGTCAAAACA GCTCATCGCT AACCTGTGAC 
TTTACTGACA ATGTTACCAT TTTTGGTTCT TACCAACTTC GTGTTCGGAC AGAACTAGAT 
GGAGAAACAT CTGATTGGGT GGAGACTAAA CAAGTTGCAG TGGACAAAAT AACTGAGATT 
AGTGCTCCTC ATGTAGAACT GAGAAGTAAC AATGGACAAA CAGAAGTTGA TATCACTGAT 
CCACCGATGA AAAGAAAAAA CCTGAGAGAC GTCTTTGGGA ATATTTCTTA CCTCATACGT 
TTTTGGAAAA AAGGAGAATC AAATAAGGTC AAACTAAAAA GAGAACAGAG CAGAGTCATG 
TTACCAAAAG TGAAGCCTCT GGAAAACTAC TGTGTCGAAG TAGAGGTTTT GTATTTGAAG 
AATAAAACAA GCCAGCCCAG CAACATGACC TGTTTAAAAA ATTCGCCCAG CAATGAGACA 
GAACCCTGGC TTATAGCTGT GATGCTTTTG ATCAGTTTTC TAGTGGTGTT AGTTGCGGTT 
CTCCTGATCT TTATGGCTGT GTGGTACAGT TACAAAGGAT ACAGAATTGT ATTCCCTAAT 
GATAATCTGC CAGAGCATCT AAAACAGTAT TTGGCACAAC GGTCCCAGTC ACCTGCACCT 
TTACCCATAC ATGAGGGAAC TCACCTGAAG GAGCCCATTC ATGAGTTAAT GATATATGAA 
AAGAAACTTA ACAGCTCTCA GGACAATCAG CAAATAATAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Cloning, identification of the two cytokine receptor family B subunits CRFB1 and CRFB5 from grass carp (Ctenopharyngodon idella).
Fish & shellfish immunology45(2)211-20(2015 Aug)
Chen H,Liu W,Wang B,Mao H,Sun Z,Hou Q,Mi Y,Fan L,Hu C

Atlantic salmon possesses two clusters of type I interferon receptor genes on different chromosomes, which allows for a larger repertoire of interferon receptors than in zebrafish and mammals.
Developmental and comparative immunology47(2)275-86(2014 Dec)
Sun B,Greiner-Tollersrud L,Koop BF,Robertsen B

In vivo protein trapping produces a functional expression codex of the vertebrate proteome.
Nature methods8(6)506-15(2011 Jun)
Clark KJ,Balciunas D,Pogoda HM,Ding Y,Westcot SE,Bedell VM,Greenwood TM,Urban MD,Skuster KJ,Petzold AM,Ni J,Nielsen AL,Patowary A,Scaria V,Sivasubbu S,Xu X,Hammerschmidt M,Ekker SC

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crfb4 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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