espl1 cDNA ORF clone, Danio rerio(zebrafish)

The following espl1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the espl1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa144755 XM_021471828.1
Latest version!
Danio rerio extra spindle pole bodies like 1, separase (espl1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
ODa144756 XM_021471829.1
Latest version!
Danio rerio extra spindle pole bodies like 1, separase (espl1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
ODa146569 XM_021474123.1
Latest version!
Danio rerio extra spindle pole bodies like 1, separase (espl1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
ODa146570 XM_021474124.1
Latest version!
Danio rerio extra spindle pole bodies like 1, separase (espl1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
ODa12112 XM_021477220.1
Latest version!
Danio rerio extra spindle pole bodies like 1, separase (espl1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
ODa12112 XM_001337833.5
Latest version!
Danio rerio extra spindle pole bodies like 1, separase (espl1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
ODa12112 XM_017356662.1
Latest version!
Danio rerio extra spindle pole bodies like 1, separase (espl1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
ODa12113 XM_005166094.4
Latest version!
Danio rerio extra spindle pole bodies like 1, separase (espl1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
ODa12113 XM_021477221.1
Latest version!
Danio rerio extra spindle pole bodies like 1, separase (espl1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
View Old Accession Versions >>
ODa12112 XM_001337833.4 Danio rerio extra spindle pole bodies like 1, separase (espl1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
ODa12113 XM_005166094.3 Danio rerio extra spindle pole bodies like 1, separase (espl1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa144755
    Clone ID Related Accession (Same CDS sequence) XM_021471828.1
    Accession Version XM_021471828.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6420bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product separin isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394662.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    ATGTGTGATC GTATCATCAG AGCCTGCAAC CAGCGGCTGG GCAACGGCTC TGTGGATGCT 
    GGCCTTCAGG AGCGGATAGT GGAGCTGGTG GAACTGGCTG TCCAAGGGTT TCAACTGCTT
    GAAGAGTCCG GCATGCAGAG CACCCCCTTT TATCTGGAGA AAATAGTCTT TCACATCCTA
    CAGAAAGTCG CAAGTCTTGG AGCTCATGGT CCTGCTTGTC GACTGGGTCA GCTCGTGTAT
    AGAAGACTGA AATGTTTGTC TGCAGAGATT GACGACTTTA ATGTCCTGGT GCGGAATTGC
    TTTGCAGTCT TGTGGAACAG TCTCCTCTCT AACAAACCGA GTGCTTCCAC CCTCCCTCCA
    CGAGACAGAC TCCACTGTCA GCTGCAAGCT TTAATATTCA GACTCCTTGA ACAGGGCTCC
    AGCTCGTTCT CTTCCCCATC CAAAGTTCCT CTGTTTGTTG AGGAAGCAGT AGTGGAGTAC
    GAACGATCAT GTGGAGGTTT TACTCAAGAC GATGTGAGTT TTCTGACCTC TGAATTGCGT
    GAGCTGTTCT CTGGTGCTTT ATTCAATCCT CAGGACTCTG CATCAACTTC TTCACTTCTG
    GCTGTGTGGT GTGAGGTTGC ATTTAAAGTG TCTAAACTGC TCTGCAAAAG TGGCTTTTCA
    CCTGAGGCTG TTGAACTCTT GCGAGGCACT CTGAAGGAGG TTAATGGCCA CGTTGGCCTG
    CGTTCGGCTC TGGGTCTGGC AGACCGGGCA GTGCAGATGC AGTGTGTGTT AAGTTCTGGA
    GGTGAATGCA GTAAGAGCTT CACTGAATGT GCCCGTGCTC TCCGGATGTT GCCTTGTGCC
    ATGTCCAGCT CAGAGTCTCA TGCCCTGTTA GAGGCTTGTC AGCTGGTGGT TTGGACGTTG
    GAAGCTAGTC AGTGTAAAGG AATGGATGTG ACCACTTTGC TGGCTTGTTT TTCCTTTTTG
    GAAGAATATC AGGAATTTCT CCTTACACAG CAAAAGAACT CGTTTTCGCT GCAGGTGCAA
    TACTCTCTTT GTTTCAGTTT TTACCAGGGC TTCATCTGCA CATATGATAG TCTTCATGCT
    TCTCAGGTGT CTGTGGGCGA GACCTTGGAC AGAGTGCTAT TGTTCTGCCG GTCGACCGCA
    GGACGTATGC TTGCTGAACT GCGGACACTC AATAATGACA ACTTGCTGCT TAAAGCAGTG
    TGTGCAGTGA ATAATGTGGT GTACGAGCTG TTTAACAGGA AGCTGTTTGA AGGAGCCTTT
    GGATTGTCGA TGATTGTGTG TCAGGAGCTC TGTAAGGACT GCCCGCCCTC TCTTCCTGTG
    GATCGGGTCA ACCGCTGTTT CATGCTGACA GTGCAGTGTA GTCGCCGCGC AGGCCATCTG
    GAGCAGGCTC TGGACTGGAT TGTTCGCTGG ATTCAGGCTT TGGGCTCACA GATTTTGGAT
    CATCTCACTG AACCTGTGTC GATGTGGGTC AAAACTAAGT GTGATGCCGC CAGAGCAGGC
    GAGGATGACA CACGCCTGAG GACTCTGAGG GACGGGTTAG GAGAATCCTT AGTGGATGAG
    GAGGTTATGG TGTGTTTGTT AGAGGAGGAG TTGCGTGTAT ATAAGGAGCA GACTGGAGAC
    ACAGCACAGG AACGCTACAA CACACTCTGC GACCTCTTGG AAATCTGCCA TGAAGAAACC
    CCACACACAC TAAGAAGAGC TACGTATCTG TGTGAGATGG CGCAGGTTGT GTGCTATCAG
    GACTTCAGCC AGCAGACTGA CTGCTCAGCA GTTGATTTTA CCCACGAGGC TTTGCGGTTG
    CTGGAAGCAG AACCAGAGAC GGCAGAGAAT ACTGATAGGC TGAAGGATGA GAAAGCCCAC
    GCTTCCCTGT GGCTCTATAT CTGCACTCTT GAGTCTAACC TGCAGGAGGC TGTGGACACA
    GAGAAGCGTT TGTGTGCAGT GCAGGAGGAG AATAAAACAG ATGTGAATAT GGATCCTGTT
    CCCACAAATG ATCTGGAGTA CGAGGACAAA CAGAAATCAC AAGAGAGCCA GTTAGTCTAC
    GATGGACTAC GCTTCAACCT GCTGGCTCAC AGCAAGCAAA GTGAACCTCT AGATAGATGT
    TTGTCTCTAT GGCGGAGTCT CCTCAGAGGG TCTGTGCCTG CAGTTAGAGA CCCTAAACTC
    ACAGCTTCTT CCATGACTTT GATGGCTGCT CTATACACAC TTATGGGCAA ACACCTGCAA
    GCTCTGGAGG GATATCAGCT TGCTGCCACC CTTTTCCATG TACTTGGTGA TGGTCAGAAT
    AGTGCCAATG CCTTCTGCCA CTCTACCAGG ATCTTGCTTT ACCTGGGTTC ACCGCAGCTT
    GCACAGGTGG ACTTAGAAAA AGCAGAAAAG AGCTTGTCAT CCGTCACCAT CTCTGAGGGC
    ACATCTATTA TCTCCATGCT GGCCATGTTG CAAAAAGCAC AGCTACACTA TGCTCTAGGA
    CAGGTGGAGA GTGGCGTGTC CTGCCTGATG GAGGTGATAA AGGAATCTGC TCAGCATCAT
    TCTAAGAGCT GGTACCTGCT CAGGGCTCGA GCACTGCAGA CAGCCAGTGA ATACTTGAGT
    CTGGACACAC TGGCTTTGAA CTCACAGCTA CGCCAGATCA TCATGCAGCA GGGTCTAAAG
    ACTCCAGACA CTGCTCAATA TGAAGGGTTG AAGCTGTTGT GTAGCCTGGT GATGATGTTA
    TTGGGCAACG GGTTTTACGG AGCGCCTGGA CCAAACACTG ACACTCGCTT TGTGGATAAA
    GGAGACAGTG TCGTGTTTAA GTGGTTGTTG TTGAGTGAAG TGCTGGTGTG CTCGGAGAGA
    ATGGTGATCA CCAGGAGCAG CAGCGGAGCC GTTCATGAAG CTAAAGCTCA GTGTCTAGAG
    GCTCTCAAAC TGGCCACCAA ACTGCAGACA CTCAGCCACT GTGCTGAGCT GTTGGTGCTG
    AAGGCAGAGC TGGAGCTGAT GAAGGGTGCT ACTGAAGCTA GTAGTTTAGA CCTGGAGCAA
    GTTAGACATT TACTTGATCT CTGCACAGAT TTTGGCCAGG GACGACAACA GAAATCTGAA
    GTGAAAATCA AACCACGCAA AGGTCGTCCC GCTGCCCCTT CTTCCACTGG TCACATCTTT
    GAGGAAGAGG ATGATCTGAG TGGGATCCTC AGCTCTCGTG CCCTTCCGAA GGAGCCTGTG
    GAGAACATGG CTAGATTAGG AAGCCAAGGA GCTTCTCCCC CTCTAAAGCC CAAACGCCAG
    CATGGCCTCT CCTGTCTGAC CCATGAAGAG ACCTGCTCTT GTCTCTGCTG CACTGAGCTC
    AGCGTGGCTC GAGTCAGCAT CCACTGGGCA CTATTACAGG CAGATCTCCA GACAGATCCC
    GAACGCTCTC TCAGACTACG CCAGTCTGCC AGGAAACGCT GCCGCTATGT TGTAACTAAA
    CTTCAGAGAA GTTTGGCAGC ACTTGTACAC TCCAAAAAAT CACCTAATGT CTGCTTGTCA
    CTGCTTCAGG CTGAACAGGG TAAAGTGCAT CTGGGTGCTG TGCTTCAACT GCTCAGAACT
    GGAGACAAAG GGAAAGCTGC CATCTTGTGG GAGGAGATAG AAGCAGGATT AGAGGCAGTG
    ACACCTAAAG GAGCACTGAC ACCAGAACTT GGGCCTATCA GAGCTGCTCT ACTGGGGGCT
    AAAGCCGTAG CCTGCTGCTT GGCGTTGGCC ATGAAGAAAC AGTGTATACC CGAAGAGCTG
    TTTTCTTCAT TGTGGCACTG GAATCCAACA AAGGTTAAAC CGCAATTAAA ATCTAAGCAT
    CGATCCAAGA GTCCGGACGC AGCTCCTCTA GAGCCAGGTG TTAAACATAA CCCTAAAGTT
    AAGAGTGGAC TTGAGCAGAA TGAAAGCAGA AAAAAAGAGA CCACTGTCAT TTCGAAGAAA
    GCTAAAGACT CTGTGCCCAA AATTACCATC ACAAAATCCT CCATGGTTTT TAAAACTCCC
    AAAGCAACGA GAACAACCCG GCCCAAATCG GTCTCCACTA GCACTGGCAT CAGTGACCTG
    AGAGCATTTG ACTTCAATAA TGAGGTGCCT GACATTTCAG TCAACTTCAC ACCATCATTA
    CACCGATCTA CAAACCAAAG TGCGACTGCA AAGTCAAAAG CGTCCTCAAA GGGGTCGTTC
    CAGGTTTACA AAGATTCTTC TCCTGCTGCA GAGAAAGCTG TAGTTGTCCC GGCTGCTCCT
    AAACGAACAA AGCGTTCTCG CTTCAAGGTT GAGTTCAGCG ATGAAAGCGA CAACGATGCT
    GCTCCTCTTG CTGTAATGGA AAAGTCAGAG AAAAAGAAAA ACACCTTTAA AACATCACGC
    ACCTTGAAAC CAACCCTTAA CACCATCCCT AGTGACACCA AACTTGTGAG CTCCACAGTA
    GATCCAGAAC CCTCCAGACG AACACGGACC ACTAAGAAAA GCACAGCTCT TCCCTCCATT
    GGTGGCCTCT CATCTGAGGA AGAACTTGGA GTATCTTCAC AGACACGTAC GCGTAGAGGA
    CGACCAAAGA AGAGCACCAG CTCAGCAGAC ACAGAGGAGC CAGAGAGGAT GAGGATGATT
    AAGGAGGAAG AACAGAGGGT TCTTTTGAAT GTCAGCATGG AGGAGCTCAG AGGCTCTGAC
    ACCGAGACAA AAGACACTGG AAGTCCAGAT GCTGACTGTG AGGTCTTGAG GAGAGATCTA
    GGTGCAGATT TTGGGCAAGA GTGTTTCAGT GAGCTCAGGA GAGCTGGACA GAATGGATCA
    GGATCTCACA CAGCTCTGCC ACATGCAACT ACATCTCCTG CAGACTTGTC AGTAGAGACG
    GTCCAGTCAT ACCTGCGCTC CTCATGGCTC CTCCTGCACC ACTTCCCGTT TCCTTCTCTT
    TATCCTCACA TCTGCTCATT ACTCGCTCAG TCATTCGGTC AAACGGACCC CATCATCACA
    GCAATGCTGC ATGCACAGGC ACTGGGAGTG TCCACTCGCC ACCATATGAC CCGTCACGTG
    GTTAACCAGT TCAGGAAGTT GAAGAAATCC TGTAACGATG TAGCAGAAGG TCTTGGAGCT
    CTGAGTCTGG AGGAAACATC TGGGGCAACG CAGTCAAAGA CACTCTCTGC GTTGGAGCAG
    ATCTTTTCAT TCACAGCAGC AAACCCAATG CAGTTTCCAC AGACACACTG TCAGCAGTTC
    ACACAGCAGC TGGAAGATCT ACCTGCAGGA GTGACCGTAT GCTTGCTGTC ACTGACGGGG
    GTGCATCCTG ATGAAATTGG CAACACTATT CTTCTGACCC GACTGGAGCG CGGCTCTACC
    CCAATCACAG TTCGAATCCC CACTGCAGAC AGAGAGCAAT CTGTTGCTGG CCTGTTGGAA
    GAAATGGATG GAGTCTTGAA AGGACAAAAG GAAGTGAGCA CAGTGGCTGA GAAATCACAG
    TGGTGGGACG GAAGGAAGGC TTTAGATGCC CGCGTGGAGA AAATGCTGGA GGAGATGGAA
    GAGGCTTTAG GAGTGTGGCG AACTCTTCTT CTGCCTTTGA CCTCTGATCC TAAGCTTGAT
    GTTCAGGTAA AGAGCCTCCA AAAGGCTCTT AAATCAACAA AGATCACACG GGACACTCTC
    AAGGTGATTC TTTCAGCGTC TCCACTGCTG TCCCTGCCAG ACCTGCAGTG TCTTCTGGAA
    GGAATGAATG TGCAGGACAA GGACCTGCTG AAGCTCCTGC AGAGGGCCGT GGCTGAACTG
    AGAGGGAGGG AGGAGCAACA GGGACACACT GTTCTGATTT TAGACAAGTA CTTGCAGAGG
    TTGCCGTGGG AGAACATTGC TTGTCTGAAG TCTCAGTCGG TCACTCGAAT GCCCTCATTA
    CACGCGGTGC TTGGACACAG TCATCTGAGA GAGGTGGATA CAAGTTGTGT TCTGTCTTGT
    GGTGTGAACC CTAAGCAGGT TTACTATGTG CTAAACCCAG ATGGAAACCT GCCCGACACT
    GAGAATCGCT TCAAAGAGTG GTTTACAGGT GAACGAACAT GGCAGGGGGT TTGTGGAAGG
    GCTCCTGATC CAGAGAAACT ACAAGAGGCT GTGACCACTA AGGATCTCTA CATATACATC
    GGACATGGTG CAGGAGCCCG CTTTCTGGAT GCGCAGAGAG TTTTGAAGGT GCCTGTGAGG
    GCCGCAGCTC TTCTGTTCGG CTGCAGTAGT GCTGCTCTCA GTGTGCTTGG TCAGCAGGAG
    GGAACGGGCA TCATCCTCAG CTACCTGACC GCTGGATGTC CATTGGTGCT GGGTAACCTG
    TGGGATGTGA CAGATCGTGA CTTGGATCGA TTCACATCGG CTCTGCTCCA GTCTTGGCTC
    TCTGCTGGTT CTGGCTCTTC ACTTCTTCAG CATTTGGCGA AATCACGCAG CGCAACTCAT
    TTAAAACATA TAATCGGTGC TGCGCCCATT GCGTATGGTT TACCGGTGCA TATACACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021327503.1
    CDS59..6478
    Translation

    Target ORF information:

    RefSeq Version XM_021471828.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio extra spindle pole bodies like 1, separase (espl1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021471828.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    ATGTGTGATC GTATCATCAG AGCCTGCAAC CAGCGGCTGG GCAACGGCTC TGTGGATGCT 
    GGCCTTCAGG AGCGGATAGT GGAGCTGGTG GAACTGGCTG TCCAAGGGTT TCAACTGCTT
    GAAGAGTCCG GCATGCAGAG CACCCCCTTT TATCTGGAGA AAATAGTCTT TCACATCCTA
    CAGAAAGTCG CAAGTCTTGG AGCTCATGGT CCTGCTTGTC GACTGGGTCA GCTCGTGTAT
    AGAAGACTGA AATGTTTGTC TGCAGAGATT GACGACTTTA ATGTCCTGGT GCGGAATTGC
    TTTGCAGTCT TGTGGAACAG TCTCCTCTCT AACAAACCGA GTGCTTCCAC CCTCCCTCCA
    CGAGACAGAC TCCACTGTCA GCTGCAAGCT TTAATATTCA GACTCCTTGA ACAGGGCTCC
    AGCTCGTTCT CTTCCCCATC CAAAGTTCCT CTGTTTGTTG AGGAAGCAGT AGTGGAGTAC
    GAACGATCAT GTGGAGGTTT TACTCAAGAC GATGTGAGTT TTCTGACCTC TGAATTGCGT
    GAGCTGTTCT CTGGTGCTTT ATTCAATCCT CAGGACTCTG CATCAACTTC TTCACTTCTG
    GCTGTGTGGT GTGAGGTTGC ATTTAAAGTG TCTAAACTGC TCTGCAAAAG TGGCTTTTCA
    CCTGAGGCTG TTGAACTCTT GCGAGGCACT CTGAAGGAGG TTAATGGCCA CGTTGGCCTG
    CGTTCGGCTC TGGGTCTGGC AGACCGGGCA GTGCAGATGC AGTGTGTGTT AAGTTCTGGA
    GGTGAATGCA GTAAGAGCTT CACTGAATGT GCCCGTGCTC TCCGGATGTT GCCTTGTGCC
    ATGTCCAGCT CAGAGTCTCA TGCCCTGTTA GAGGCTTGTC AGCTGGTGGT TTGGACGTTG
    GAAGCTAGTC AGTGTAAAGG AATGGATGTG ACCACTTTGC TGGCTTGTTT TTCCTTTTTG
    GAAGAATATC AGGAATTTCT CCTTACACAG CAAAAGAACT CGTTTTCGCT GCAGGTGCAA
    TACTCTCTTT GTTTCAGTTT TTACCAGGGC TTCATCTGCA CATATGATAG TCTTCATGCT
    TCTCAGGTGT CTGTGGGCGA GACCTTGGAC AGAGTGCTAT TGTTCTGCCG GTCGACCGCA
    GGACGTATGC TTGCTGAACT GCGGACACTC AATAATGACA ACTTGCTGCT TAAAGCAGTG
    TGTGCAGTGA ATAATGTGGT GTACGAGCTG TTTAACAGGA AGCTGTTTGA AGGAGCCTTT
    GGATTGTCGA TGATTGTGTG TCAGGAGCTC TGTAAGGACT GCCCGCCCTC TCTTCCTGTG
    GATCGGGTCA ACCGCTGTTT CATGCTGACA GTGCAGTGTA GTCGCCGCGC AGGCCATCTG
    GAGCAGGCTC TGGACTGGAT TGTTCGCTGG ATTCAGGCTT TGGGCTCACA GATTTTGGAT
    CATCTCACTG AACCTGTGTC GATGTGGGTC AAAACTAAGT GTGATGCCGC CAGAGCAGGC
    GAGGATGACA CACGCCTGAG GACTCTGAGG GACGGGTTAG GAGAATCCTT AGTGGATGAG
    GAGGTTATGG TGTGTTTGTT AGAGGAGGAG TTGCGTGTAT ATAAGGAGCA GACTGGAGAC
    ACAGCACAGG AACGCTACAA CACACTCTGC GACCTCTTGG AAATCTGCCA TGAAGAAACC
    CCACACACAC TAAGAAGAGC TACGTATCTG TGTGAGATGG CGCAGGTTGT GTGCTATCAG
    GACTTCAGCC AGCAGACTGA CTGCTCAGCA GTTGATTTTA CCCACGAGGC TTTGCGGTTG
    CTGGAAGCAG AACCAGAGAC GGCAGAGAAT ACTGATAGGC TGAAGGATGA GAAAGCCCAC
    GCTTCCCTGT GGCTCTATAT CTGCACTCTT GAGTCTAACC TGCAGGAGGC TGTGGACACA
    GAGAAGCGTT TGTGTGCAGT GCAGGAGGAG AATAAAACAG ATGTGAATAT GGATCCTGTT
    CCCACAAATG ATCTGGAGTA CGAGGACAAA CAGAAATCAC AAGAGAGCCA GTTAGTCTAC
    GATGGACTAC GCTTCAACCT GCTGGCTCAC AGCAAGCAAA GTGAACCTCT AGATAGATGT
    TTGTCTCTAT GGCGGAGTCT CCTCAGAGGG TCTGTGCCTG CAGTTAGAGA CCCTAAACTC
    ACAGCTTCTT CCATGACTTT GATGGCTGCT CTATACACAC TTATGGGCAA ACACCTGCAA
    GCTCTGGAGG GATATCAGCT TGCTGCCACC CTTTTCCATG TACTTGGTGA TGGTCAGAAT
    AGTGCCAATG CCTTCTGCCA CTCTACCAGG ATCTTGCTTT ACCTGGGTTC ACCGCAGCTT
    GCACAGGTGG ACTTAGAAAA AGCAGAAAAG AGCTTGTCAT CCGTCACCAT CTCTGAGGGC
    ACATCTATTA TCTCCATGCT GGCCATGTTG CAAAAAGCAC AGCTACACTA TGCTCTAGGA
    CAGGTGGAGA GTGGCGTGTC CTGCCTGATG GAGGTGATAA AGGAATCTGC TCAGCATCAT
    TCTAAGAGCT GGTACCTGCT CAGGGCTCGA GCACTGCAGA CAGCCAGTGA ATACTTGAGT
    CTGGACACAC TGGCTTTGAA CTCACAGCTA CGCCAGATCA TCATGCAGCA GGGTCTAAAG
    ACTCCAGACA CTGCTCAATA TGAAGGGTTG AAGCTGTTGT GTAGCCTGGT GATGATGTTA
    TTGGGCAACG GGTTTTACGG AGCGCCTGGA CCAAACACTG ACACTCGCTT TGTGGATAAA
    GGAGACAGTG TCGTGTTTAA GTGGTTGTTG TTGAGTGAAG TGCTGGTGTG CTCGGAGAGA
    ATGGTGATCA CCAGGAGCAG CAGCGGAGCC GTTCATGAAG CTAAAGCTCA GTGTCTAGAG
    GCTCTCAAAC TGGCCACCAA ACTGCAGACA CTCAGCCACT GTGCTGAGCT GTTGGTGCTG
    AAGGCAGAGC TGGAGCTGAT GAAGGGTGCT ACTGAAGCTA GTAGTTTAGA CCTGGAGCAA
    GTTAGACATT TACTTGATCT CTGCACAGAT TTTGGCCAGG GACGACAACA GAAATCTGAA
    GTGAAAATCA AACCACGCAA AGGTCGTCCC GCTGCCCCTT CTTCCACTGG TCACATCTTT
    GAGGAAGAGG ATGATCTGAG TGGGATCCTC AGCTCTCGTG CCCTTCCGAA GGAGCCTGTG
    GAGAACATGG CTAGATTAGG AAGCCAAGGA GCTTCTCCCC CTCTAAAGCC CAAACGCCAG
    CATGGCCTCT CCTGTCTGAC CCATGAAGAG ACCTGCTCTT GTCTCTGCTG CACTGAGCTC
    AGCGTGGCTC GAGTCAGCAT CCACTGGGCA CTATTACAGG CAGATCTCCA GACAGATCCC
    GAACGCTCTC TCAGACTACG CCAGTCTGCC AGGAAACGCT GCCGCTATGT TGTAACTAAA
    CTTCAGAGAA GTTTGGCAGC ACTTGTACAC TCCAAAAAAT CACCTAATGT CTGCTTGTCA
    CTGCTTCAGG CTGAACAGGG TAAAGTGCAT CTGGGTGCTG TGCTTCAACT GCTCAGAACT
    GGAGACAAAG GGAAAGCTGC CATCTTGTGG GAGGAGATAG AAGCAGGATT AGAGGCAGTG
    ACACCTAAAG GAGCACTGAC ACCAGAACTT GGGCCTATCA GAGCTGCTCT ACTGGGGGCT
    AAAGCCGTAG CCTGCTGCTT GGCGTTGGCC ATGAAGAAAC AGTGTATACC CGAAGAGCTG
    TTTTCTTCAT TGTGGCACTG GAATCCAACA AAGGTTAAAC CGCAATTAAA ATCTAAGCAT
    CGATCCAAGA GTCCGGACGC AGCTCCTCTA GAGCCAGGTG TTAAACATAA CCCTAAAGTT
    AAGAGTGGAC TTGAGCAGAA TGAAAGCAGA AAAAAAGAGA CCACTGTCAT TTCGAAGAAA
    GCTAAAGACT CTGTGCCCAA AATTACCATC ACAAAATCCT CCATGGTTTT TAAAACTCCC
    AAAGCAACGA GAACAACCCG GCCCAAATCG GTCTCCACTA GCACTGGCAT CAGTGACCTG
    AGAGCATTTG ACTTCAATAA TGAGGTGCCT GACATTTCAG TCAACTTCAC ACCATCATTA
    CACCGATCTA CAAACCAAAG TGCGACTGCA AAGTCAAAAG CGTCCTCAAA GGGGTCGTTC
    CAGGTTTACA AAGATTCTTC TCCTGCTGCA GAGAAAGCTG TAGTTGTCCC GGCTGCTCCT
    AAACGAACAA AGCGTTCTCG CTTCAAGGTT GAGTTCAGCG ATGAAAGCGA CAACGATGCT
    GCTCCTCTTG CTGTAATGGA AAAGTCAGAG AAAAAGAAAA ACACCTTTAA AACATCACGC
    ACCTTGAAAC CAACCCTTAA CACCATCCCT AGTGACACCA AACTTGTGAG CTCCACAGTA
    GATCCAGAAC CCTCCAGACG AACACGGACC ACTAAGAAAA GCACAGCTCT TCCCTCCATT
    GGTGGCCTCT CATCTGAGGA AGAACTTGGA GTATCTTCAC AGACACGTAC GCGTAGAGGA
    CGACCAAAGA AGAGCACCAG CTCAGCAGAC ACAGAGGAGC CAGAGAGGAT GAGGATGATT
    AAGGAGGAAG AACAGAGGGT TCTTTTGAAT GTCAGCATGG AGGAGCTCAG AGGCTCTGAC
    ACCGAGACAA AAGACACTGG AAGTCCAGAT GCTGACTGTG AGGTCTTGAG GAGAGATCTA
    GGTGCAGATT TTGGGCAAGA GTGTTTCAGT GAGCTCAGGA GAGCTGGACA GAATGGATCA
    GGATCTCACA CAGCTCTGCC ACATGCAACT ACATCTCCTG CAGACTTGTC AGTAGAGACG
    GTCCAGTCAT ACCTGCGCTC CTCATGGCTC CTCCTGCACC ACTTCCCGTT TCCTTCTCTT
    TATCCTCACA TCTGCTCATT ACTCGCTCAG TCATTCGGTC AAACGGACCC CATCATCACA
    GCAATGCTGC ATGCACAGGC ACTGGGAGTG TCCACTCGCC ACCATATGAC CCGTCACGTG
    GTTAACCAGT TCAGGAAGTT GAAGAAATCC TGTAACGATG TAGCAGAAGG TCTTGGAGCT
    CTGAGTCTGG AGGAAACATC TGGGGCAACG CAGTCAAAGA CACTCTCTGC GTTGGAGCAG
    ATCTTTTCAT TCACAGCAGC AAACCCAATG CAGTTTCCAC AGACACACTG TCAGCAGTTC
    ACACAGCAGC TGGAAGATCT ACCTGCAGGA GTGACCGTAT GCTTGCTGTC ACTGACGGGG
    GTGCATCCTG ATGAAATTGG CAACACTATT CTTCTGACCC GACTGGAGCG CGGCTCTACC
    CCAATCACAG TTCGAATCCC CACTGCAGAC AGAGAGCAAT CTGTTGCTGG CCTGTTGGAA
    GAAATGGATG GAGTCTTGAA AGGACAAAAG GAAGTGAGCA CAGTGGCTGA GAAATCACAG
    TGGTGGGACG GAAGGAAGGC TTTAGATGCC CGCGTGGAGA AAATGCTGGA GGAGATGGAA
    GAGGCTTTAG GAGTGTGGCG AACTCTTCTT CTGCCTTTGA CCTCTGATCC TAAGCTTGAT
    GTTCAGGTAA AGAGCCTCCA AAAGGCTCTT AAATCAACAA AGATCACACG GGACACTCTC
    AAGGTGATTC TTTCAGCGTC TCCACTGCTG TCCCTGCCAG ACCTGCAGTG TCTTCTGGAA
    GGAATGAATG TGCAGGACAA GGACCTGCTG AAGCTCCTGC AGAGGGCCGT GGCTGAACTG
    AGAGGGAGGG AGGAGCAACA GGGACACACT GTTCTGATTT TAGACAAGTA CTTGCAGAGG
    TTGCCGTGGG AGAACATTGC TTGTCTGAAG TCTCAGTCGG TCACTCGAAT GCCCTCATTA
    CACGCGGTGC TTGGACACAG TCATCTGAGA GAGGTGGATA CAAGTTGTGT TCTGTCTTGT
    GGTGTGAACC CTAAGCAGGT TTACTATGTG CTAAACCCAG ATGGAAACCT GCCCGACACT
    GAGAATCGCT TCAAAGAGTG GTTTACAGGT GAACGAACAT GGCAGGGGGT TTGTGGAAGG
    GCTCCTGATC CAGAGAAACT ACAAGAGGCT GTGACCACTA AGGATCTCTA CATATACATC
    GGACATGGTG CAGGAGCCCG CTTTCTGGAT GCGCAGAGAG TTTTGAAGGT GCCTGTGAGG
    GCCGCAGCTC TTCTGTTCGG CTGCAGTAGT GCTGCTCTCA GTGTGCTTGG TCAGCAGGAG
    GGAACGGGCA TCATCCTCAG CTACCTGACC GCTGGATGTC CATTGGTGCT GGGTAACCTG
    TGGGATGTGA CAGATCGTGA CTTGGATCGA TTCACATCGG CTCTGCTCCA GTCTTGGCTC
    TCTGCTGGTT CTGGCTCTTC ACTTCTTCAG CATTTGGCGA AATCACGCAG CGCAACTCAT
    TTAAAACATA TAATCGGTGC TGCGCCCATT GCGTATGGTT TACCGGTGCA TATACACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa144756
    Clone ID Related Accession (Same CDS sequence) XM_021471829.1
    Accession Version XM_021471829.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6417bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product separin isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394662.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    ATGTGTGATC GTATCATCAG AGCCTGCAAC CAGCGGCTGG GCAACGGCTC TGTGGATGCT 
    GGCCTTCAGG AGCGGATAGT GGAGCTGGTG GAACTGGCTG TCCAAGGGTT TCAACTGCTT
    GAAGAGTCCG GCATGCAGAG CACCCCCTTT TATCTGGAGA AAATAGTCTT TCACATCCTA
    CAGAAAGTCG CAAGTCTTGG AGCTCATGGT CCTGCTTGTC GACTGGGTCA GCTCGTGTAT
    AGAAGACTGA AATGTTTGTC TGCAGAGATT GACGACTTTA ATGTCCTGGT GCGGAATTGC
    TTTGCAGTCT TGTGGAACAG TCTCCTCTCT AACAAACCGA GTGCTTCCAC CCTCCCTCCA
    CGAGACAGAC TCCACTGTCA GCTGCAAGCT TTAATATTCA GACTCCTTGA ACAGGGCTCC
    AGCTCGTTCT CTTCCCCATC CAAAGTTCCT CTGTTTGTTG AGGAAGCAGT AGTGGAGTAC
    GAACGATCAT GTGGAGGTTT TACTCAAGAC GATGTGAGTT TTCTGACCTC TGAATTGCGT
    GAGCTGTTCT CTGGTGCTTT ATTCAATCCT CAGGACTCTG CATCAACTTC TTCACTTCTG
    GCTGTGTGGT GTGAGGTTGC ATTTAAAGTG TCTAAACTGC TCTGCAAAAG TGGCTTTTCA
    CCTGAGGCTG TTGAACTCTT GCGAGGCACT CTGAAGGAGG TTAATGGCCA CGTTGGCCTG
    CGTTCGGCTC TGGGTCTGGC AGACCGGGCA GTGCAGATGC AGTGTGTGTT AAGTTCTGGA
    GGTGAATGCA GTAAGAGCTT CACTGAATGT GCCCGTGCTC TCCGGATGTT GCCTTGTGCC
    ATGTCCAGCT CAGAGTCTCA TGCCCTGTTA GAGGCTTGTC AGCTGGTGGT TTGGACGTTG
    GAAGCTAGTC AGTGTAAAGG AATGGATGTG ACCACTTTGC TGGCTTGTTT TTCCTTTTTG
    GAAGAATATC AGGAATTTCT CCTTACACAG CAAAAGAACT CGTTTTCGCT GCAGGTGCAA
    TACTCTCTTT GTTTCAGTTT TTACCAGGGC TTCATCTGCA CATATGATAG TCTTCATGCT
    TCTCAGGTGT CTGTGGGCGA GACCTTGGAC AGAGTGCTAT TGTTCTGCCG GTCGACCGCA
    GGACGTATGC TTGCTGAACT GCGGACACTC AATAATGACA ACTTGCTGCT TAAAGCAGTG
    TGTGCAGTGA ATAATGTGGT GTACGAGCTG TTTAACAGGA AGCTGTTTGA AGGAGCCTTT
    GGATTGTCGA TGATTGTGTG TCAGGAGCTC TGTAAGGACT GCCCGCCCTC TCTTCCTGTG
    GATCGGGTCA ACCGCTGTTT CATGCTGACA GTGCAGTGTA GTCGCCGCGC AGGCCATCTG
    GAGCAGGCTC TGGACTGGAT TGTTCGCTGG ATTCAGGCTT TGGGCTCACA GATTTTGGAT
    CATCTCACTG AACCTGTGTC GATGTGGGTC AAAACTAAGT GTGATGCCGC CAGAGCAGGC
    GAGGATGACA CACGCCTGAG GACTCTGAGG GACGGGTTAG GAGAATCCTT AGTGGATGAG
    GAGGTTATGG TGTGTTTGTT AGAGGAGGAG TTGCGTGTAT ATAAGGAGCA GACTGGAGAC
    ACAGCACAGG AACGCTACAA CACACTCTGC GACCTCTTGG AAATCTGCCA TGAAGAAACC
    CCACACACAC TAAGAAGAGC TACGTATCTG TGTGAGATGG CGCAGGTTGT GTGCTATCAG
    GACTTCAGCC AGCAGACTGA CTGCTCAGCA GTTGATTTTA CCCACGAGGC TTTGCGGTTG
    CTGGAAGCAG AACCAGAGAC GGCAGAGAAT ACTGATAGGC TGAAGGATGA GAAAGCCCAC
    GCTTCCCTGT GGCTCTATAT CTGCACTCTT GAGTCTAACC TGCAGGAGGC TGTGGACACA
    GAGAAGCGTT TGTGTGCAGT GCAGGAGGAG AATAAAACAG ATGTGAATAT GGATCCTGTT
    CCCACAAATG ATCTGGAGTA CGAGGACAAA CAGAAATCAC AAGAGAGCCA GTTAGTCTAC
    GATGGACTAC GCTTCAACCT GCTGGCTCAC AGCAAGCAAA GTGAACCTCT AGATAGATGT
    TTGTCTCTAT GGCGGAGTCT CCTCAGAGGG TCTGTGCCTG CAGTTAGAGA CCCTAAACTC
    ACAGCTTCTT CCATGACTTT GATGGCTGCT CTATACACAC TTATGGGCAA ACACCTGCAA
    GCTCTGGAGG GATATCAGCT TGCTGCCACC CTTTTCCATG TACTTGGTGA TGGTCAGAAT
    AGTGCCAATG CCTTCTGCCA CTCTACCAGG ATCTTGCTTT ACCTGGGTTC ACCGCAGCTT
    GCACAGGTGG ACTTAGAAAA AGCAGAAAAG AGCTTGTCAT CCGTCACCAT CTCTGAGGGC
    ACATCTATTA TCTCCATGCT GGCCATGTTG CAAAAAGCAC AGCTACACTA TGCTCTAGGA
    CAGGTGGAGA GTGGCGTGTC CTGCCTGATG GAGGTGATAA AGGAATCTGC TCAGCATCAT
    TCTAAGAGCT GGTACCTGCT CAGGGCTCGA GCACTGCAGA CAGCCAGTGA ATACTTGAGT
    CTGGACACAC TGGCTTTGAA CTCACAGCTA CGCCAGATCA TCATGCAGCA GGGTCTAAAG
    ACTCCAGACA CTGCTCAATA TGAAGGGTTG AAGCTGTTGT GTAGCCTGGT GATGATGTTA
    TTGGGCAACG GGTTTTACGG AGCGCCTGGA CCAAACACTG ACACTCGCTT TGTGGATAAA
    GGAGACAGTG TCGTGTTTAA GTGGTTGTTG TTGAGTGAAG TGCTGGTGTG CTCGGAGAGA
    ATGGTGATCA CCAGGAGCAG CAGCGGAGCC GTTCATGAAG CTAAAGCTCA GTGTCTAGAG
    GCTCTCAAAC TGGCCACCAA ACTGCAGACA CTCAGCCACT GTGCTGAGCT GTTGGTGCTG
    AAGGCAGAGC TGGAGCTGAT GAAGGGTGCT ACTGAAGCTA GTAGTTTAGA CCTGGAGCAA
    GTTAGACATT TACTTGATCT CTGCACAGAT TTTGGCCAGG GACGACAACA GAAATCTGAA
    GTGAAAATCA AACCACGCAA AGGTCGTCCC GCTGCCCCTT CTTCCACTGG TCACATCTTT
    GAGGAAGAGG ATGATCTGAG TGGGATCCTC AGCTCTCGTG CCCTTCCGAA GGAGCCTGTG
    GAGAACATGG CTAGATTAGG AAGCCAAGGA GCTTCTCCCC CTCTAAAGCC CAAACGCCAG
    CATGGCCTCT CCTGTCTGAC CCATGAAGAG ACCTGCTCTT GTCTCTGCTG CACTGAGCTC
    AGCGTGGCTC GAGTCAGCAT CCACTGGGCA CTATTACAGG CAGATCTCCA GACAGATCCC
    GAACGCTCTC TCAGACTACG CCAGTCTGCC AGGAAACGCT GCCGCTATGT TGTAACTAAA
    CTTCAGAGAA GTTTGGCAGC ACTTGTACAC TCCAAAAAAT CACCTAATGT CTGCTTGTCA
    CTGCTTCAGG CTGAACAGGG TAAAGTGCAT CTGGGTGCTG TGCTTCAACT GCTCAGAACT
    GGAGACAAAG GGAAAGCTGC CATCTTGTGG GAGGAGATAG AAGCAGGATT AGAGGCAGTG
    ACACCTAAAG GAGCACTGAC ACCAGAACTT GGGCCTATCA GAGCTGCTCT ACTGGGGGCT
    AAAGCCGTAG CCTGCTGCTT GGCGTTGGCC ATGAAGAAAC AGTGTATACC CGAAGAGCTG
    TTTTCTTCAT TGTGGCACTG GAATCCAACA AAGGTTAAAC CGCAATTAAA ATCTAAGCAT
    CGATCCAAGA GTCCGGACGC AGCTCCTCTA GAGCCAGGTG TTAAACATAA CCCTAAAGTT
    AAGAGTGGAC TTGAGCAGAA TGAAAGCAGA AAAAAAGAGA CCACTGTCAT TTCGAAGAAA
    GCTAAAGACT CTGTGCCCAA AATTACCATC ACAAAATCCT CCATGGTTTT TAAAACTCCC
    AAAGCAACGA GAACAACCCG GCCCAAATCG GTCTCCACTA GCACTGGCAT CAGTGACCTG
    AGAGCATTTG ACTTCAATAA TGAGGTGCCT GACATTTCAG TCAACTTCAC ACCATCATTA
    CACCGATCTA CAAACCAAAG TGCGACTGCA AAGTCAAAAG CGTCCTCAAA GGGGTCGTTC
    CAGGTTTACA AAGATTCTTC TCCTGCTGCA GAGAAAGCTG TAGTTGTCCC GGCTGCTCCT
    AAACGAACAA AGCGTTCTCG CTTCAAGGTT GAGTTCAGCG ATGAAAGCGA CAACGATGCT
    GCTCCTCTTG CTGTAATGGA AAAGTCAGAG AAAAAGAAAA ACACCTTTAA AACATCACGC
    ACCTTGAAAC CAACCCTTAA CACCATCCCT AGTGACACCA AACTTGTGAG CTCCACAGTA
    GATCCAGAAC CCTCCAGACG AACACGGACC ACTAAGAAAA GCACAGCTCT TCCCTCCATT
    GGTGGCCTCT CATCTGAGGA AGAACTTGGA GTATCTTCAC AGACACGTAC GCGTAGAGGA
    CGACCAAAGA AGAGCACCAG CTCAGCAGAC ACAGAGGAGC CAGAGAGGAT GAGGATGATT
    AAGGAGGAAG AACAGAGGGT TCTTTTGAAT GTCAGCATGG AGGAGCTCAG AGGCTCTGAC
    ACCGAGACAA AAGACACTGG AAGTCCAGAT GCTGACTGTG AGGTCTTGAG GAGAGATCTA
    GGTGCAGATT TTGGGCAAGA GTGTTTCAGT GAGCTCAGGA GAGCTGGACA GAATGGATCA
    GGATCTCACA CAGCTCTGCC ACATGCAACT ACATCTCCTG ACTTGTCAGT AGAGACGGTC
    CAGTCATACC TGCGCTCCTC ATGGCTCCTC CTGCACCACT TCCCGTTTCC TTCTCTTTAT
    CCTCACATCT GCTCATTACT CGCTCAGTCA TTCGGTCAAA CGGACCCCAT CATCACAGCA
    ATGCTGCATG CACAGGCACT GGGAGTGTCC ACTCGCCACC ATATGACCCG TCACGTGGTT
    AACCAGTTCA GGAAGTTGAA GAAATCCTGT AACGATGTAG CAGAAGGTCT TGGAGCTCTG
    AGTCTGGAGG AAACATCTGG GGCAACGCAG TCAAAGACAC TCTCTGCGTT GGAGCAGATC
    TTTTCATTCA CAGCAGCAAA CCCAATGCAG TTTCCACAGA CACACTGTCA GCAGTTCACA
    CAGCAGCTGG AAGATCTACC TGCAGGAGTG ACCGTATGCT TGCTGTCACT GACGGGGGTG
    CATCCTGATG AAATTGGCAA CACTATTCTT CTGACCCGAC TGGAGCGCGG CTCTACCCCA
    ATCACAGTTC GAATCCCCAC TGCAGACAGA GAGCAATCTG TTGCTGGCCT GTTGGAAGAA
    ATGGATGGAG TCTTGAAAGG ACAAAAGGAA GTGAGCACAG TGGCTGAGAA ATCACAGTGG
    TGGGACGGAA GGAAGGCTTT AGATGCCCGC GTGGAGAAAA TGCTGGAGGA GATGGAAGAG
    GCTTTAGGAG TGTGGCGAAC TCTTCTTCTG CCTTTGACCT CTGATCCTAA GCTTGATGTT
    CAGGTAAAGA GCCTCCAAAA GGCTCTTAAA TCAACAAAGA TCACACGGGA CACTCTCAAG
    GTGATTCTTT CAGCGTCTCC ACTGCTGTCC CTGCCAGACC TGCAGTGTCT TCTGGAAGGA
    ATGAATGTGC AGGACAAGGA CCTGCTGAAG CTCCTGCAGA GGGCCGTGGC TGAACTGAGA
    GGGAGGGAGG AGCAACAGGG ACACACTGTT CTGATTTTAG ACAAGTACTT GCAGAGGTTG
    CCGTGGGAGA ACATTGCTTG TCTGAAGTCT CAGTCGGTCA CTCGAATGCC CTCATTACAC
    GCGGTGCTTG GACACAGTCA TCTGAGAGAG GTGGATACAA GTTGTGTTCT GTCTTGTGGT
    GTGAACCCTA AGCAGGTTTA CTATGTGCTA AACCCAGATG GAAACCTGCC CGACACTGAG
    AATCGCTTCA AAGAGTGGTT TACAGGTGAA CGAACATGGC AGGGGGTTTG TGGAAGGGCT
    CCTGATCCAG AGAAACTACA AGAGGCTGTG ACCACTAAGG ATCTCTACAT ATACATCGGA
    CATGGTGCAG GAGCCCGCTT TCTGGATGCG CAGAGAGTTT TGAAGGTGCC TGTGAGGGCC
    GCAGCTCTTC TGTTCGGCTG CAGTAGTGCT GCTCTCAGTG TGCTTGGTCA GCAGGAGGGA
    ACGGGCATCA TCCTCAGCTA CCTGACCGCT GGATGTCCAT TGGTGCTGGG TAACCTGTGG
    GATGTGACAG ATCGTGACTT GGATCGATTC ACATCGGCTC TGCTCCAGTC TTGGCTCTCT
    GCTGGTTCTG GCTCTTCACT TCTTCAGCAT TTGGCGAAAT CACGCAGCGC AACTCATTTA
    AAACATATAA TCGGTGCTGC GCCCATTGCG TATGGTTTAC CGGTGCATAT ACACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021327504.1
    CDS59..6475
    Translation

    Target ORF information:

    RefSeq Version XM_021471829.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio extra spindle pole bodies like 1, separase (espl1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021471829.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    ATGTGTGATC GTATCATCAG AGCCTGCAAC CAGCGGCTGG GCAACGGCTC TGTGGATGCT 
    GGCCTTCAGG AGCGGATAGT GGAGCTGGTG GAACTGGCTG TCCAAGGGTT TCAACTGCTT
    GAAGAGTCCG GCATGCAGAG CACCCCCTTT TATCTGGAGA AAATAGTCTT TCACATCCTA
    CAGAAAGTCG CAAGTCTTGG AGCTCATGGT CCTGCTTGTC GACTGGGTCA GCTCGTGTAT
    AGAAGACTGA AATGTTTGTC TGCAGAGATT GACGACTTTA ATGTCCTGGT GCGGAATTGC
    TTTGCAGTCT TGTGGAACAG TCTCCTCTCT AACAAACCGA GTGCTTCCAC CCTCCCTCCA
    CGAGACAGAC TCCACTGTCA GCTGCAAGCT TTAATATTCA GACTCCTTGA ACAGGGCTCC
    AGCTCGTTCT CTTCCCCATC CAAAGTTCCT CTGTTTGTTG AGGAAGCAGT AGTGGAGTAC
    GAACGATCAT GTGGAGGTTT TACTCAAGAC GATGTGAGTT TTCTGACCTC TGAATTGCGT
    GAGCTGTTCT CTGGTGCTTT ATTCAATCCT CAGGACTCTG CATCAACTTC TTCACTTCTG
    GCTGTGTGGT GTGAGGTTGC ATTTAAAGTG TCTAAACTGC TCTGCAAAAG TGGCTTTTCA
    CCTGAGGCTG TTGAACTCTT GCGAGGCACT CTGAAGGAGG TTAATGGCCA CGTTGGCCTG
    CGTTCGGCTC TGGGTCTGGC AGACCGGGCA GTGCAGATGC AGTGTGTGTT AAGTTCTGGA
    GGTGAATGCA GTAAGAGCTT CACTGAATGT GCCCGTGCTC TCCGGATGTT GCCTTGTGCC
    ATGTCCAGCT CAGAGTCTCA TGCCCTGTTA GAGGCTTGTC AGCTGGTGGT TTGGACGTTG
    GAAGCTAGTC AGTGTAAAGG AATGGATGTG ACCACTTTGC TGGCTTGTTT TTCCTTTTTG
    GAAGAATATC AGGAATTTCT CCTTACACAG CAAAAGAACT CGTTTTCGCT GCAGGTGCAA
    TACTCTCTTT GTTTCAGTTT TTACCAGGGC TTCATCTGCA CATATGATAG TCTTCATGCT
    TCTCAGGTGT CTGTGGGCGA GACCTTGGAC AGAGTGCTAT TGTTCTGCCG GTCGACCGCA
    GGACGTATGC TTGCTGAACT GCGGACACTC AATAATGACA ACTTGCTGCT TAAAGCAGTG
    TGTGCAGTGA ATAATGTGGT GTACGAGCTG TTTAACAGGA AGCTGTTTGA AGGAGCCTTT
    GGATTGTCGA TGATTGTGTG TCAGGAGCTC TGTAAGGACT GCCCGCCCTC TCTTCCTGTG
    GATCGGGTCA ACCGCTGTTT CATGCTGACA GTGCAGTGTA GTCGCCGCGC AGGCCATCTG
    GAGCAGGCTC TGGACTGGAT TGTTCGCTGG ATTCAGGCTT TGGGCTCACA GATTTTGGAT
    CATCTCACTG AACCTGTGTC GATGTGGGTC AAAACTAAGT GTGATGCCGC CAGAGCAGGC
    GAGGATGACA CACGCCTGAG GACTCTGAGG GACGGGTTAG GAGAATCCTT AGTGGATGAG
    GAGGTTATGG TGTGTTTGTT AGAGGAGGAG TTGCGTGTAT ATAAGGAGCA GACTGGAGAC
    ACAGCACAGG AACGCTACAA CACACTCTGC GACCTCTTGG AAATCTGCCA TGAAGAAACC
    CCACACACAC TAAGAAGAGC TACGTATCTG TGTGAGATGG CGCAGGTTGT GTGCTATCAG
    GACTTCAGCC AGCAGACTGA CTGCTCAGCA GTTGATTTTA CCCACGAGGC TTTGCGGTTG
    CTGGAAGCAG AACCAGAGAC GGCAGAGAAT ACTGATAGGC TGAAGGATGA GAAAGCCCAC
    GCTTCCCTGT GGCTCTATAT CTGCACTCTT GAGTCTAACC TGCAGGAGGC TGTGGACACA
    GAGAAGCGTT TGTGTGCAGT GCAGGAGGAG AATAAAACAG ATGTGAATAT GGATCCTGTT
    CCCACAAATG ATCTGGAGTA CGAGGACAAA CAGAAATCAC AAGAGAGCCA GTTAGTCTAC
    GATGGACTAC GCTTCAACCT GCTGGCTCAC AGCAAGCAAA GTGAACCTCT AGATAGATGT
    TTGTCTCTAT GGCGGAGTCT CCTCAGAGGG TCTGTGCCTG CAGTTAGAGA CCCTAAACTC
    ACAGCTTCTT CCATGACTTT GATGGCTGCT CTATACACAC TTATGGGCAA ACACCTGCAA
    GCTCTGGAGG GATATCAGCT TGCTGCCACC CTTTTCCATG TACTTGGTGA TGGTCAGAAT
    AGTGCCAATG CCTTCTGCCA CTCTACCAGG ATCTTGCTTT ACCTGGGTTC ACCGCAGCTT
    GCACAGGTGG ACTTAGAAAA AGCAGAAAAG AGCTTGTCAT CCGTCACCAT CTCTGAGGGC
    ACATCTATTA TCTCCATGCT GGCCATGTTG CAAAAAGCAC AGCTACACTA TGCTCTAGGA
    CAGGTGGAGA GTGGCGTGTC CTGCCTGATG GAGGTGATAA AGGAATCTGC TCAGCATCAT
    TCTAAGAGCT GGTACCTGCT CAGGGCTCGA GCACTGCAGA CAGCCAGTGA ATACTTGAGT
    CTGGACACAC TGGCTTTGAA CTCACAGCTA CGCCAGATCA TCATGCAGCA GGGTCTAAAG
    ACTCCAGACA CTGCTCAATA TGAAGGGTTG AAGCTGTTGT GTAGCCTGGT GATGATGTTA
    TTGGGCAACG GGTTTTACGG AGCGCCTGGA CCAAACACTG ACACTCGCTT TGTGGATAAA
    GGAGACAGTG TCGTGTTTAA GTGGTTGTTG TTGAGTGAAG TGCTGGTGTG CTCGGAGAGA
    ATGGTGATCA CCAGGAGCAG CAGCGGAGCC GTTCATGAAG CTAAAGCTCA GTGTCTAGAG
    GCTCTCAAAC TGGCCACCAA ACTGCAGACA CTCAGCCACT GTGCTGAGCT GTTGGTGCTG
    AAGGCAGAGC TGGAGCTGAT GAAGGGTGCT ACTGAAGCTA GTAGTTTAGA CCTGGAGCAA
    GTTAGACATT TACTTGATCT CTGCACAGAT TTTGGCCAGG GACGACAACA GAAATCTGAA
    GTGAAAATCA AACCACGCAA AGGTCGTCCC GCTGCCCCTT CTTCCACTGG TCACATCTTT
    GAGGAAGAGG ATGATCTGAG TGGGATCCTC AGCTCTCGTG CCCTTCCGAA GGAGCCTGTG
    GAGAACATGG CTAGATTAGG AAGCCAAGGA GCTTCTCCCC CTCTAAAGCC CAAACGCCAG
    CATGGCCTCT CCTGTCTGAC CCATGAAGAG ACCTGCTCTT GTCTCTGCTG CACTGAGCTC
    AGCGTGGCTC GAGTCAGCAT CCACTGGGCA CTATTACAGG CAGATCTCCA GACAGATCCC
    GAACGCTCTC TCAGACTACG CCAGTCTGCC AGGAAACGCT GCCGCTATGT TGTAACTAAA
    CTTCAGAGAA GTTTGGCAGC ACTTGTACAC TCCAAAAAAT CACCTAATGT CTGCTTGTCA
    CTGCTTCAGG CTGAACAGGG TAAAGTGCAT CTGGGTGCTG TGCTTCAACT GCTCAGAACT
    GGAGACAAAG GGAAAGCTGC CATCTTGTGG GAGGAGATAG AAGCAGGATT AGAGGCAGTG
    ACACCTAAAG GAGCACTGAC ACCAGAACTT GGGCCTATCA GAGCTGCTCT ACTGGGGGCT
    AAAGCCGTAG CCTGCTGCTT GGCGTTGGCC ATGAAGAAAC AGTGTATACC CGAAGAGCTG
    TTTTCTTCAT TGTGGCACTG GAATCCAACA AAGGTTAAAC CGCAATTAAA ATCTAAGCAT
    CGATCCAAGA GTCCGGACGC AGCTCCTCTA GAGCCAGGTG TTAAACATAA CCCTAAAGTT
    AAGAGTGGAC TTGAGCAGAA TGAAAGCAGA AAAAAAGAGA CCACTGTCAT TTCGAAGAAA
    GCTAAAGACT CTGTGCCCAA AATTACCATC ACAAAATCCT CCATGGTTTT TAAAACTCCC
    AAAGCAACGA GAACAACCCG GCCCAAATCG GTCTCCACTA GCACTGGCAT CAGTGACCTG
    AGAGCATTTG ACTTCAATAA TGAGGTGCCT GACATTTCAG TCAACTTCAC ACCATCATTA
    CACCGATCTA CAAACCAAAG TGCGACTGCA AAGTCAAAAG CGTCCTCAAA GGGGTCGTTC
    CAGGTTTACA AAGATTCTTC TCCTGCTGCA GAGAAAGCTG TAGTTGTCCC GGCTGCTCCT
    AAACGAACAA AGCGTTCTCG CTTCAAGGTT GAGTTCAGCG ATGAAAGCGA CAACGATGCT
    GCTCCTCTTG CTGTAATGGA AAAGTCAGAG AAAAAGAAAA ACACCTTTAA AACATCACGC
    ACCTTGAAAC CAACCCTTAA CACCATCCCT AGTGACACCA AACTTGTGAG CTCCACAGTA
    GATCCAGAAC CCTCCAGACG AACACGGACC ACTAAGAAAA GCACAGCTCT TCCCTCCATT
    GGTGGCCTCT CATCTGAGGA AGAACTTGGA GTATCTTCAC AGACACGTAC GCGTAGAGGA
    CGACCAAAGA AGAGCACCAG CTCAGCAGAC ACAGAGGAGC CAGAGAGGAT GAGGATGATT
    AAGGAGGAAG AACAGAGGGT TCTTTTGAAT GTCAGCATGG AGGAGCTCAG AGGCTCTGAC
    ACCGAGACAA AAGACACTGG AAGTCCAGAT GCTGACTGTG AGGTCTTGAG GAGAGATCTA
    GGTGCAGATT TTGGGCAAGA GTGTTTCAGT GAGCTCAGGA GAGCTGGACA GAATGGATCA
    GGATCTCACA CAGCTCTGCC ACATGCAACT ACATCTCCTG ACTTGTCAGT AGAGACGGTC
    CAGTCATACC TGCGCTCCTC ATGGCTCCTC CTGCACCACT TCCCGTTTCC TTCTCTTTAT
    CCTCACATCT GCTCATTACT CGCTCAGTCA TTCGGTCAAA CGGACCCCAT CATCACAGCA
    ATGCTGCATG CACAGGCACT GGGAGTGTCC ACTCGCCACC ATATGACCCG TCACGTGGTT
    AACCAGTTCA GGAAGTTGAA GAAATCCTGT AACGATGTAG CAGAAGGTCT TGGAGCTCTG
    AGTCTGGAGG AAACATCTGG GGCAACGCAG TCAAAGACAC TCTCTGCGTT GGAGCAGATC
    TTTTCATTCA CAGCAGCAAA CCCAATGCAG TTTCCACAGA CACACTGTCA GCAGTTCACA
    CAGCAGCTGG AAGATCTACC TGCAGGAGTG ACCGTATGCT TGCTGTCACT GACGGGGGTG
    CATCCTGATG AAATTGGCAA CACTATTCTT CTGACCCGAC TGGAGCGCGG CTCTACCCCA
    ATCACAGTTC GAATCCCCAC TGCAGACAGA GAGCAATCTG TTGCTGGCCT GTTGGAAGAA
    ATGGATGGAG TCTTGAAAGG ACAAAAGGAA GTGAGCACAG TGGCTGAGAA ATCACAGTGG
    TGGGACGGAA GGAAGGCTTT AGATGCCCGC GTGGAGAAAA TGCTGGAGGA GATGGAAGAG
    GCTTTAGGAG TGTGGCGAAC TCTTCTTCTG CCTTTGACCT CTGATCCTAA GCTTGATGTT
    CAGGTAAAGA GCCTCCAAAA GGCTCTTAAA TCAACAAAGA TCACACGGGA CACTCTCAAG
    GTGATTCTTT CAGCGTCTCC ACTGCTGTCC CTGCCAGACC TGCAGTGTCT TCTGGAAGGA
    ATGAATGTGC AGGACAAGGA CCTGCTGAAG CTCCTGCAGA GGGCCGTGGC TGAACTGAGA
    GGGAGGGAGG AGCAACAGGG ACACACTGTT CTGATTTTAG ACAAGTACTT GCAGAGGTTG
    CCGTGGGAGA ACATTGCTTG TCTGAAGTCT CAGTCGGTCA CTCGAATGCC CTCATTACAC
    GCGGTGCTTG GACACAGTCA TCTGAGAGAG GTGGATACAA GTTGTGTTCT GTCTTGTGGT
    GTGAACCCTA AGCAGGTTTA CTATGTGCTA AACCCAGATG GAAACCTGCC CGACACTGAG
    AATCGCTTCA AAGAGTGGTT TACAGGTGAA CGAACATGGC AGGGGGTTTG TGGAAGGGCT
    CCTGATCCAG AGAAACTACA AGAGGCTGTG ACCACTAAGG ATCTCTACAT ATACATCGGA
    CATGGTGCAG GAGCCCGCTT TCTGGATGCG CAGAGAGTTT TGAAGGTGCC TGTGAGGGCC
    GCAGCTCTTC TGTTCGGCTG CAGTAGTGCT GCTCTCAGTG TGCTTGGTCA GCAGGAGGGA
    ACGGGCATCA TCCTCAGCTA CCTGACCGCT GGATGTCCAT TGGTGCTGGG TAACCTGTGG
    GATGTGACAG ATCGTGACTT GGATCGATTC ACATCGGCTC TGCTCCAGTC TTGGCTCTCT
    GCTGGTTCTG GCTCTTCACT TCTTCAGCAT TTGGCGAAAT CACGCAGCGC AACTCATTTA
    AAACATATAA TCGGTGCTGC GCCCATTGCG TATGGTTTAC CGGTGCATAT ACACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa146569
    Clone ID Related Accession (Same CDS sequence) XM_021474123.1
    Accession Version XM_021474123.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6546bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product separin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395261.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    ATGGCATTAA AGAGCTTGAA AACAGAAGAT TACGTTCAGC AGTTATCCTG TGTCAAGGAC 
    ACCATAGTCC TCCACAACGA ACTGAAGAAA CATGTGGATG GTGGCTTGGG TCCTTATGGT
    CGCACAATGT GTGATCGTAT CATCAGAGCC TGCAACCAGC GGCTGGGCAA CGGCTCTGTG
    GATGCTGGCC TTCAGGAGCG GATAGTGGAG CTGGTGGAAC TGGCTGTCCA AGGGTTTCAA
    CTGCTTGAAG AGTCCGGCAT GCAGAGCACC CCCTTTTATC TGGAGAAAAT AGTCTTTCAC
    ATCCTACAGA AAGTCGCAAG TCTTGGAGCT CATGGTCCTG CTTGTCGACT GGGTCAGCTC
    GTGTATAGAA GACTGAAATG TTTGTCTGCA GAGATTGACG ACTTTAATGT CCTGGTGCGG
    AATTGCTTTG CAGTCTTGTG GAACAGTCTC CTCTCTAACA AACCGAGTGC TTCCACCCTC
    CCTCCACGAG ACAGACTCCA CTGTCAGCTG CAAGCTGTAA TATTCAGACT CCTTGAACAG
    GGCTCCAGCT TGTCCTCTTC CCCATCCAAA GTTCCTCTGT TTGTTGAGGA AGCAGTAGTG
    GAGTACGAAC GATCATGTGG AGGTTTTACT CAAGACGATG TGAGTTTTCT GACCTCTGAA
    TTGCGTGAGC TGTTCTCTGG TGCTTTATTC AATCCTCAGG ACTCTGCATC AACTTCTTCA
    CTGCTGGCTG TGTGGTGTGA GGTGGCATTT AAAGTGTCTA AACTGCTCTG CAAAAGTGGC
    TTTTCACCTG AGGCTGTTGA ACTCTTGCGA GGCACTCTGA AGGAGGTTAA TGGCCACGTT
    GGCCTGCGTT CGGCTCTGGG TCTGGCAGAC CGGGCAGTGC AGATGCAGTG TGTGTTAAGT
    TCTGGAGGTG AATGCAGTAA GAGCTTTACT GAATGTGCCC GTGCTCTCCG GATGTTGCCT
    TGTGCCATGT CCAGCTCAGA GTCTCATGCC CTGTTAGAGG CTTGTCAGCT GGTGGTTTGG
    ACGTTGGAAG CTAGTCAGTG TAAAGGAATG GATGTGACCA CTTTGCTGGC TTGTTTTTCC
    TTTTTGGAAG AATATCAGGA ATTTCTCCCT ACACAGCAAA AGAACTCGTT TTCGCTGCAG
    GTGCAATACT CTCTTTGTTT CAGTTTTTAC CAGGGCTTCA TCTGCACGTA TGATAGTCTT
    CATGCATCTC AGGTGTCTGT GGGCGAGACC TTGGACAGAG TGCTATTGTT CTGCCGGTCG
    ACCGCAGGAC GTATGCTTGC TGAACTGCGG ACACTCAATA ATGACAACTT GCTGCTTAAA
    GCAGTGTGTG CAGTGAATAA TGTGGTGTAC GAGCTGTTTA ACAGGAAGCT GTATGAAGGA
    GCCTTTGGAT TGTCGATGAT TGTGTGTCAG GAGCTCTGTA AGGACTGCCC GCCCTCTCTT
    CCTGTGGATC GGGTCAACCG CTGTTTCATG CTGACAGTGC AGTGTAGTCG CCGCGCAGGC
    CATCTGGAGA AGGCTCTGGA TTGGATTGTT CGCTGGATTC AGGCTTTGGG CTCACAGATT
    TTGGATCATC TCACTGAACC TGTGTCGATG TGGGTCAAAA CTAAGTGTGA TGCCGCCAGA
    GCAGGCGAGG ATGACACACG CCTGAGGACT CTGAGGGACG GGTTAGGAGA ATCCTTAGTG
    GATGAGGAGG TTATGGTGTG TTTGTTAGAG GAGGAGTTGC GTGTATATAA GGAGCAGACT
    GGAGACACAG CACAGGAACG CTACAACACA CTCTGCGACC TCTTGGAAAT CTGCCATGAA
    GAAACCCCAC GCACACTAAG AAGAGCTACG TATCTGTGTG AGATGGCGCA GGTTGTGTGC
    TATCAGGACT TCAGCCAGCA GACTGACTGC TCAGCAGTTG ATTTTACCCA CGAGGCTTTG
    CGGTTGCTGG AAGCAGAACC AGAGACGGCA GAGAATACTG ATAGGCTGAA GGATGAGAAA
    GCCCACGCTT CCCTGTGGCT CTATATCTGC ACTCTTGAGT CTAACCTGCA GGAGGCTGTG
    GACACAGAGA AGCGTTTGTG TGCAGTGCAG GAGGAGAATA AAAGAGATGT GAATATGGAT
    CCTGTTCCCA CAAATGATCT GGAGTACGAG GACAAACAGA AATCACAAGA GAGCCAGTTA
    GTCTACGATG GACTACGCTT CAACCTGCTG GCTCACAGCA AGCAAAGTGA ACCTCTAGAT
    AGATGTTTGT CTCTATGGCG GAGTCTCCTC AGAGGGTCTG TGCCTGCAGT TAGAGACCCT
    AAACTCACAG CTTCTTCCAT GACTTTGATG GCTGCTCTAT ACACACTTAT GGGCAAACAC
    CTGCAAGCTC TGGAGGGATA TCAGCTTGCT GCCACCCTTT TCCATGTACT TGGTGATGGT
    CAGAATAGTG CCAATGCCTT CTGCCACTCT ACCAGGATCT TGCTTTACCT GGGTTCGCCG
    CAGCTTGCAC AGGTGGACTT AGAAAAAGCA GAAAAGAGCT TGTCATCCGT CACCATCTCT
    GAGGGCACAT CTATTATCTC CATGCTGGCC ATGTTGCAAA AAGCACAGCT ACACTATGCT
    CTAGGACAGG TGGAGAGTGG CGTGTCCTGC CTGATGGAGG TGATAAAGGA ATCTGCTCAG
    CATCATTCTA AGAGCTGGTA CCTCCTCAGG GCTCGAGCAC TGCAGACAGC CAGTGAATAC
    TTGAGTCTGG ACACACTGGC TTTGAACTCA CAGCTACGCC AGATCATCAT GCAGCAGGGT
    CTAAAGACTC CAGACACTGC TCAATATGAA GGGTTGAAGC TGTTGTGTAG CCTGGTGATG
    ATGTTATTGG GCAACGGGTT TTACGGAGCG CCTGGACCAA ACACTGACAC TCGCTTTGTG
    GATAAAGGAG ACAGTGTTGT GTTTAAGTGG TTGTTGCTGA GTGAAGTGCT GGTGTGCTCG
    GAGAGAATGG TGATCACCAG GAGCAGCAGC GGAGCCGTTC ATGAAGCTAA AGCTCAGTGT
    CTAGAGGCTC TCAAACTGGC CACCAAACTG CAGACACTCA GCCACTGTGC TGAGCTGTTG
    GTGCTGAAGG CAGAGCTGGA GCTGATGAAG GGTGCTACTG AAGCTAGTAG TTTAGACCTG
    GAGCAAGTTA GACATTTACT TGATCTCTGC ACAGATTTTG GCCAGGGACG ACAACAGAAA
    TCTGAAGTGA AAATCAAACC ACGCAAAGGT CGTCCCGCTG CCCCTTCTTC CTCTGGTCAC
    ATCTCTGAGG AAGAGGATGA TCTGAGTGGG ATCCTCAGCT CTCGTGCCCT TCCGAAGGAG
    CCTGTGGAGA ACATGGCTAG ATTAGGAAGC CAAGGAGCTT CTCCCCCTCT AAAGCCCAAA
    CGCCAGCATG GCCTCTCCTG TCTGACCCAT GAAGAGACCT GCTCTTGTCT CTGCTGCACT
    GAGCTCAGCG TGGCTCGAGT CAGCATCCAC TGGGCACTAT TACAGGCAGA TCTCCAGACA
    GATCCCGAAC ACTCTCTCAG ACTACGCCAG TCTGCCAGGA AACGCTGCCG CTATGTTGTA
    ACTAAACTTC AGAGAAGTTT GGCAGCACTT GTACACTTCA AAAAATCAAC TAATGTCTGC
    TTGTCACTGC TTCAGGCTGA ACAGGGTAAA GTGCATCTGG GTGCTGTGCT TCAACTGCTC
    AGAACTGGAG ACAAAGGGAA AGCTGCCATC TTGTGGGAGG AGATAGAAGC AGGATTAGAG
    GCAGTGACAC CTAAAGGAGC ACTGACACCA GAACTTGGGC CTATCAGAGC TGCTCTACTG
    GGGGCTAAAG CCGTAGCCTG CTGCTTGGCG TTGGCCATGA AGAAACAGTG TATACCCGAA
    GAGCTGTTTT CTTCATTGTG GCACTGGAAT GCAACAAAGG TTAAACCGCA ATTAAAATCT
    AAGCATCGAT CCAAGAGTCC GGACGCAGCT CCTCTAGAGC CAGGTGTTAA ACATAACCCT
    AAAGTTAAGA GTGGACTTGA GGAGAATGAA AGCAGAAAAA AAGAGACCAC TGTCATTTCG
    AAGAAAGCTA AAGACTCTGT GCCCAAGATT ACTATTACAA AATCCTCCAT GGTTTTTAAA
    ACGCCCAAAG CAACGAGAAC AACCCGGCCC AAATCTGTCT CCACTAGCAC TGGCATCAGT
    GACCTGAGAG CATTTGACTT CAATAATGAG GTGCCTGACA TTTCAGTCAA CTTCACACCA
    TCATTACACC GATCTACAAA CCAAAGTGCG ACTGCAAAGT CAAAAGCGTC CTCAAAGGGG
    TCGTTCCAGG TTTACAAAGA TTCTTCTCCT GCTGCAGAGA AAGCTGTAGT TGTCCCGGCT
    GCTCCTAAAC GAACAAAGCG TTCTCGCTTC AAGGTTGAGT TCAGCGATGA AAGCGACACC
    GAAGCTGCTC CTCTTGCTGT AATGGAAAAG TCAGAGAAAA AGAAAAACAC CTCTAAAACA
    TCACGCACCT TGAAACCAAC CCTTAACACC ATCCCTAGTG ACACCAAACT TTTGAGCTCC
    ACAGTAGATC CAGAACCCTC CAGACGAACA CGGACCACTA AGAAAAGCAC AGCTCTTCCC
    TCCATTGGTG GCCTCTCTTC TGAGGAAGAA CTTGGAGTAT CTTCACAGAC ACGTACGCGT
    AGAGGACGAC CAAAGAAGAG CACCAGCTCA GCAGACACAG AGGAGCCAGA GAGGATGAGG
    ATGATTAAGG AGGAAGAACA GAGGGTTCTT TTGAATGTCA GCATGGAGGA GCTCAGAGGC
    TCTGACACCG AGACAAAAGA CACTGGAAGT CCAGATGCTG ACTGTGAGGT CTTGAGGAGA
    GATCTAGGTG CAGATTTTGG GCGAGAGTGT TTCAGTGAGC TCAGGAGAGC TGGACAGAAT
    GGATCAGGAT CTCACACAGC TCTGCCACAT GCAACTACAT CTCCTGCAGA CTTGTCAGTA
    GAGACGGTCC AGTCATACCT GCGCTCCTCA TGGCTCCTCC TGCACCACTT CCCGTTTCCT
    TCTCTTTATC CTCACATCTG CTCATTACTC GCTCAGTCAT TCGGTCAAAC GGACCCCATC
    ATCACAGCAA TGCTGCATGC ACAGGCACTG GGAGTGTCCA CTCGCCACCA TATGACCCGT
    CACGTGGTTA ACCAGTTCAG AAAGTTGAAG AAATCCTGTA ACGATGTAGC AGAAGGTCTT
    GGAGCTCTGA GTCTGGAGGA AACATCTGGA GCAACGCAGT CAAAGAAACT CTCTGCGTTG
    GAGCAGATCT TTTCATTCAC AGCATCAAAC CCAATGCAGT TTCCACAGAC ACACTGTCAG
    CAGTTCACAC AGCAGCTGGA AGATCTACCT GCAGGAGTGA CTGTATGCTT GCTGTCACTG
    ACGGGGGTGC ATCCTGATGA AATTGGCAAC ACTATTCTTC TGACCCGACT GGAGCGCGGC
    TCTACCCCAA TCACAGTTCG AATCCCCACT GCAGACAGAG AGCAATCTGT TGCTGGCCTG
    TTGGAAGAAA TGGATGGAGT CTTGAAAGGA CAAAAGGAAG TGAGCACAGT GGCTGAGAAA
    TCACAGTGGT GGGACGGAAG GAAGGCTTTA GATGCCCGTG TGGAGAAAAT GCTGGAGGAG
    ATGGAAGAGG CTTTAGGAGT GTGGCGAACT CTTCTTCTGC CTTTGACCTC TGATCCTAAG
    CTTGATGTAC AGGTAAAGAG CCTCCAAAAG GCTCTTAAAT CAACAAAGAT CACACAGGAT
    ACTCTCAAGG TGATTCTTTC AGCCTCTCCA CTGCTGTCCC TGCCAGACCT GCAGTGTCTT
    CTGGAAGGAA TGAATGTGCA GGACAAGGAC CTGCTGAAGC TCCTTCAGAG AGGCGTGGCT
    GAACTGAGAG GGAGGGAGGA GCCACAGGGA CACACTGTTC TGATTTTAGA CAAGTACTTG
    CAGAGGTTGC CGTGGGAGAA CATTGCTTGT CTGAAGTCTC AGTCGGTCAC TCGAATGCCC
    TCATTACATG CGGTGCTTGG ACACAGTCAT CTGAGAGAGG TGGATACAAG TTGTGTTCTG
    TCTTGTGGTG TGAACCCTAA GCAGGTTTAC TATGTGCTAA ACCCAGATGG AAACCTGCCC
    GACACTGAGA ATCGCTTCAA AGAGTGGTTT ACAGGTGAAC GAACATGGCA GGGGGTTTGT
    GGAAGGGCTC CTGATCCGGA GAAACTACAA GAGGCTGTGA CCACTAAAGA TCTCTACATA
    TACATCGGAC ATGGTGCAGG AGCCCGCTTT CTGGATGCGC AGAGAGTTTT GAAGGTGCCT
    GTGAGGGCCG CAGCTCTTCT GTTCGGCTGC AGTAGTGCTG CTCTCAGTGT GCTTGGTCAG
    CAGGAGGGAA CGGGCATCAT CCTCAGCTAC CTGACCGCTG GATGTCCAAT GGTGCTGGGT
    AACCTGTGGG ATGTGACAGA TCGTGACTTG GATCGATTCA CATCGGCTCT GCTCCAGTCT
    TGGCTCTCTG CTGGTTCTGG CTCTTCACTT CTTCAGCATT TGGCGAAATC ACGCAGCGCA
    ACTCATTTAA AACACATAAT CGGTGCTGCG CCCATTGCGT ATGGTTTACC GGTGCATATA
    CACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021329798.1
    CDS221..6766
    Translation

    Target ORF information:

    RefSeq Version XM_021474123.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio extra spindle pole bodies like 1, separase (espl1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021474123.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    ATGGCATTAA AGAGCTTGAA AACAGAAGAT TACGTTCAGC AGTTATCCTG TGTCAAGGAC 
    ACCATAGTCC TCCACAACGA ACTGAAGAAA CATGTGGATG GTGGCTTGGG TCCTTATGGT
    CGCACAATGT GTGATCGTAT CATCAGAGCC TGCAACCAGC GGCTGGGCAA CGGCTCTGTG
    GATGCTGGCC TTCAGGAGCG GATAGTGGAG CTGGTGGAAC TGGCTGTCCA AGGGTTTCAA
    CTGCTTGAAG AGTCCGGCAT GCAGAGCACC CCCTTTTATC TGGAGAAAAT AGTCTTTCAC
    ATCCTACAGA AAGTCGCAAG TCTTGGAGCT CATGGTCCTG CTTGTCGACT GGGTCAGCTC
    GTGTATAGAA GACTGAAATG TTTGTCTGCA GAGATTGACG ACTTTAATGT CCTGGTGCGG
    AATTGCTTTG CAGTCTTGTG GAACAGTCTC CTCTCTAACA AACCGAGTGC TTCCACCCTC
    CCTCCACGAG ACAGACTCCA CTGTCAGCTG CAAGCTGTAA TATTCAGACT CCTTGAACAG
    GGCTCCAGCT TGTCCTCTTC CCCATCCAAA GTTCCTCTGT TTGTTGAGGA AGCAGTAGTG
    GAGTACGAAC GATCATGTGG AGGTTTTACT CAAGACGATG TGAGTTTTCT GACCTCTGAA
    TTGCGTGAGC TGTTCTCTGG TGCTTTATTC AATCCTCAGG ACTCTGCATC AACTTCTTCA
    CTGCTGGCTG TGTGGTGTGA GGTGGCATTT AAAGTGTCTA AACTGCTCTG CAAAAGTGGC
    TTTTCACCTG AGGCTGTTGA ACTCTTGCGA GGCACTCTGA AGGAGGTTAA TGGCCACGTT
    GGCCTGCGTT CGGCTCTGGG TCTGGCAGAC CGGGCAGTGC AGATGCAGTG TGTGTTAAGT
    TCTGGAGGTG AATGCAGTAA GAGCTTTACT GAATGTGCCC GTGCTCTCCG GATGTTGCCT
    TGTGCCATGT CCAGCTCAGA GTCTCATGCC CTGTTAGAGG CTTGTCAGCT GGTGGTTTGG
    ACGTTGGAAG CTAGTCAGTG TAAAGGAATG GATGTGACCA CTTTGCTGGC TTGTTTTTCC
    TTTTTGGAAG AATATCAGGA ATTTCTCCCT ACACAGCAAA AGAACTCGTT TTCGCTGCAG
    GTGCAATACT CTCTTTGTTT CAGTTTTTAC CAGGGCTTCA TCTGCACGTA TGATAGTCTT
    CATGCATCTC AGGTGTCTGT GGGCGAGACC TTGGACAGAG TGCTATTGTT CTGCCGGTCG
    ACCGCAGGAC GTATGCTTGC TGAACTGCGG ACACTCAATA ATGACAACTT GCTGCTTAAA
    GCAGTGTGTG CAGTGAATAA TGTGGTGTAC GAGCTGTTTA ACAGGAAGCT GTATGAAGGA
    GCCTTTGGAT TGTCGATGAT TGTGTGTCAG GAGCTCTGTA AGGACTGCCC GCCCTCTCTT
    CCTGTGGATC GGGTCAACCG CTGTTTCATG CTGACAGTGC AGTGTAGTCG CCGCGCAGGC
    CATCTGGAGA AGGCTCTGGA TTGGATTGTT CGCTGGATTC AGGCTTTGGG CTCACAGATT
    TTGGATCATC TCACTGAACC TGTGTCGATG TGGGTCAAAA CTAAGTGTGA TGCCGCCAGA
    GCAGGCGAGG ATGACACACG CCTGAGGACT CTGAGGGACG GGTTAGGAGA ATCCTTAGTG
    GATGAGGAGG TTATGGTGTG TTTGTTAGAG GAGGAGTTGC GTGTATATAA GGAGCAGACT
    GGAGACACAG CACAGGAACG CTACAACACA CTCTGCGACC TCTTGGAAAT CTGCCATGAA
    GAAACCCCAC GCACACTAAG AAGAGCTACG TATCTGTGTG AGATGGCGCA GGTTGTGTGC
    TATCAGGACT TCAGCCAGCA GACTGACTGC TCAGCAGTTG ATTTTACCCA CGAGGCTTTG
    CGGTTGCTGG AAGCAGAACC AGAGACGGCA GAGAATACTG ATAGGCTGAA GGATGAGAAA
    GCCCACGCTT CCCTGTGGCT CTATATCTGC ACTCTTGAGT CTAACCTGCA GGAGGCTGTG
    GACACAGAGA AGCGTTTGTG TGCAGTGCAG GAGGAGAATA AAAGAGATGT GAATATGGAT
    CCTGTTCCCA CAAATGATCT GGAGTACGAG GACAAACAGA AATCACAAGA GAGCCAGTTA
    GTCTACGATG GACTACGCTT CAACCTGCTG GCTCACAGCA AGCAAAGTGA ACCTCTAGAT
    AGATGTTTGT CTCTATGGCG GAGTCTCCTC AGAGGGTCTG TGCCTGCAGT TAGAGACCCT
    AAACTCACAG CTTCTTCCAT GACTTTGATG GCTGCTCTAT ACACACTTAT GGGCAAACAC
    CTGCAAGCTC TGGAGGGATA TCAGCTTGCT GCCACCCTTT TCCATGTACT TGGTGATGGT
    CAGAATAGTG CCAATGCCTT CTGCCACTCT ACCAGGATCT TGCTTTACCT GGGTTCGCCG
    CAGCTTGCAC AGGTGGACTT AGAAAAAGCA GAAAAGAGCT TGTCATCCGT CACCATCTCT
    GAGGGCACAT CTATTATCTC CATGCTGGCC ATGTTGCAAA AAGCACAGCT ACACTATGCT
    CTAGGACAGG TGGAGAGTGG CGTGTCCTGC CTGATGGAGG TGATAAAGGA ATCTGCTCAG
    CATCATTCTA AGAGCTGGTA CCTCCTCAGG GCTCGAGCAC TGCAGACAGC CAGTGAATAC
    TTGAGTCTGG ACACACTGGC TTTGAACTCA CAGCTACGCC AGATCATCAT GCAGCAGGGT
    CTAAAGACTC CAGACACTGC TCAATATGAA GGGTTGAAGC TGTTGTGTAG CCTGGTGATG
    ATGTTATTGG GCAACGGGTT TTACGGAGCG CCTGGACCAA ACACTGACAC TCGCTTTGTG
    GATAAAGGAG ACAGTGTTGT GTTTAAGTGG TTGTTGCTGA GTGAAGTGCT GGTGTGCTCG
    GAGAGAATGG TGATCACCAG GAGCAGCAGC GGAGCCGTTC ATGAAGCTAA AGCTCAGTGT
    CTAGAGGCTC TCAAACTGGC CACCAAACTG CAGACACTCA GCCACTGTGC TGAGCTGTTG
    GTGCTGAAGG CAGAGCTGGA GCTGATGAAG GGTGCTACTG AAGCTAGTAG TTTAGACCTG
    GAGCAAGTTA GACATTTACT TGATCTCTGC ACAGATTTTG GCCAGGGACG ACAACAGAAA
    TCTGAAGTGA AAATCAAACC ACGCAAAGGT CGTCCCGCTG CCCCTTCTTC CTCTGGTCAC
    ATCTCTGAGG AAGAGGATGA TCTGAGTGGG ATCCTCAGCT CTCGTGCCCT TCCGAAGGAG
    CCTGTGGAGA ACATGGCTAG ATTAGGAAGC CAAGGAGCTT CTCCCCCTCT AAAGCCCAAA
    CGCCAGCATG GCCTCTCCTG TCTGACCCAT GAAGAGACCT GCTCTTGTCT CTGCTGCACT
    GAGCTCAGCG TGGCTCGAGT CAGCATCCAC TGGGCACTAT TACAGGCAGA TCTCCAGACA
    GATCCCGAAC ACTCTCTCAG ACTACGCCAG TCTGCCAGGA AACGCTGCCG CTATGTTGTA
    ACTAAACTTC AGAGAAGTTT GGCAGCACTT GTACACTTCA AAAAATCAAC TAATGTCTGC
    TTGTCACTGC TTCAGGCTGA ACAGGGTAAA GTGCATCTGG GTGCTGTGCT TCAACTGCTC
    AGAACTGGAG ACAAAGGGAA AGCTGCCATC TTGTGGGAGG AGATAGAAGC AGGATTAGAG
    GCAGTGACAC CTAAAGGAGC ACTGACACCA GAACTTGGGC CTATCAGAGC TGCTCTACTG
    GGGGCTAAAG CCGTAGCCTG CTGCTTGGCG TTGGCCATGA AGAAACAGTG TATACCCGAA
    GAGCTGTTTT CTTCATTGTG GCACTGGAAT GCAACAAAGG TTAAACCGCA ATTAAAATCT
    AAGCATCGAT CCAAGAGTCC GGACGCAGCT CCTCTAGAGC CAGGTGTTAA ACATAACCCT
    AAAGTTAAGA GTGGACTTGA GGAGAATGAA AGCAGAAAAA AAGAGACCAC TGTCATTTCG
    AAGAAAGCTA AAGACTCTGT GCCCAAGATT ACTATTACAA AATCCTCCAT GGTTTTTAAA
    ACGCCCAAAG CAACGAGAAC AACCCGGCCC AAATCTGTCT CCACTAGCAC TGGCATCAGT
    GACCTGAGAG CATTTGACTT CAATAATGAG GTGCCTGACA TTTCAGTCAA CTTCACACCA
    TCATTACACC GATCTACAAA CCAAAGTGCG ACTGCAAAGT CAAAAGCGTC CTCAAAGGGG
    TCGTTCCAGG TTTACAAAGA TTCTTCTCCT GCTGCAGAGA AAGCTGTAGT TGTCCCGGCT
    GCTCCTAAAC GAACAAAGCG TTCTCGCTTC AAGGTTGAGT TCAGCGATGA AAGCGACACC
    GAAGCTGCTC CTCTTGCTGT AATGGAAAAG TCAGAGAAAA AGAAAAACAC CTCTAAAACA
    TCACGCACCT TGAAACCAAC CCTTAACACC ATCCCTAGTG ACACCAAACT TTTGAGCTCC
    ACAGTAGATC CAGAACCCTC CAGACGAACA CGGACCACTA AGAAAAGCAC AGCTCTTCCC
    TCCATTGGTG GCCTCTCTTC TGAGGAAGAA CTTGGAGTAT CTTCACAGAC ACGTACGCGT
    AGAGGACGAC CAAAGAAGAG CACCAGCTCA GCAGACACAG AGGAGCCAGA GAGGATGAGG
    ATGATTAAGG AGGAAGAACA GAGGGTTCTT TTGAATGTCA GCATGGAGGA GCTCAGAGGC
    TCTGACACCG AGACAAAAGA CACTGGAAGT CCAGATGCTG ACTGTGAGGT CTTGAGGAGA
    GATCTAGGTG CAGATTTTGG GCGAGAGTGT TTCAGTGAGC TCAGGAGAGC TGGACAGAAT
    GGATCAGGAT CTCACACAGC TCTGCCACAT GCAACTACAT CTCCTGCAGA CTTGTCAGTA
    GAGACGGTCC AGTCATACCT GCGCTCCTCA TGGCTCCTCC TGCACCACTT CCCGTTTCCT
    TCTCTTTATC CTCACATCTG CTCATTACTC GCTCAGTCAT TCGGTCAAAC GGACCCCATC
    ATCACAGCAA TGCTGCATGC ACAGGCACTG GGAGTGTCCA CTCGCCACCA TATGACCCGT
    CACGTGGTTA ACCAGTTCAG AAAGTTGAAG AAATCCTGTA ACGATGTAGC AGAAGGTCTT
    GGAGCTCTGA GTCTGGAGGA AACATCTGGA GCAACGCAGT CAAAGAAACT CTCTGCGTTG
    GAGCAGATCT TTTCATTCAC AGCATCAAAC CCAATGCAGT TTCCACAGAC ACACTGTCAG
    CAGTTCACAC AGCAGCTGGA AGATCTACCT GCAGGAGTGA CTGTATGCTT GCTGTCACTG
    ACGGGGGTGC ATCCTGATGA AATTGGCAAC ACTATTCTTC TGACCCGACT GGAGCGCGGC
    TCTACCCCAA TCACAGTTCG AATCCCCACT GCAGACAGAG AGCAATCTGT TGCTGGCCTG
    TTGGAAGAAA TGGATGGAGT CTTGAAAGGA CAAAAGGAAG TGAGCACAGT GGCTGAGAAA
    TCACAGTGGT GGGACGGAAG GAAGGCTTTA GATGCCCGTG TGGAGAAAAT GCTGGAGGAG
    ATGGAAGAGG CTTTAGGAGT GTGGCGAACT CTTCTTCTGC CTTTGACCTC TGATCCTAAG
    CTTGATGTAC AGGTAAAGAG CCTCCAAAAG GCTCTTAAAT CAACAAAGAT CACACAGGAT
    ACTCTCAAGG TGATTCTTTC AGCCTCTCCA CTGCTGTCCC TGCCAGACCT GCAGTGTCTT
    CTGGAAGGAA TGAATGTGCA GGACAAGGAC CTGCTGAAGC TCCTTCAGAG AGGCGTGGCT
    GAACTGAGAG GGAGGGAGGA GCCACAGGGA CACACTGTTC TGATTTTAGA CAAGTACTTG
    CAGAGGTTGC CGTGGGAGAA CATTGCTTGT CTGAAGTCTC AGTCGGTCAC TCGAATGCCC
    TCATTACATG CGGTGCTTGG ACACAGTCAT CTGAGAGAGG TGGATACAAG TTGTGTTCTG
    TCTTGTGGTG TGAACCCTAA GCAGGTTTAC TATGTGCTAA ACCCAGATGG AAACCTGCCC
    GACACTGAGA ATCGCTTCAA AGAGTGGTTT ACAGGTGAAC GAACATGGCA GGGGGTTTGT
    GGAAGGGCTC CTGATCCGGA GAAACTACAA GAGGCTGTGA CCACTAAAGA TCTCTACATA
    TACATCGGAC ATGGTGCAGG AGCCCGCTTT CTGGATGCGC AGAGAGTTTT GAAGGTGCCT
    GTGAGGGCCG CAGCTCTTCT GTTCGGCTGC AGTAGTGCTG CTCTCAGTGT GCTTGGTCAG
    CAGGAGGGAA CGGGCATCAT CCTCAGCTAC CTGACCGCTG GATGTCCAAT GGTGCTGGGT
    AACCTGTGGG ATGTGACAGA TCGTGACTTG GATCGATTCA CATCGGCTCT GCTCCAGTCT
    TGGCTCTCTG CTGGTTCTGG CTCTTCACTT CTTCAGCATT TGGCGAAATC ACGCAGCGCA
    ACTCATTTAA AACACATAAT CGGTGCTGCG CCCATTGCGT ATGGTTTACC GGTGCATATA
    CACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa146570
    Clone ID Related Accession (Same CDS sequence) XM_021474124.1
    Accession Version XM_021474124.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6543bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product separin isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395261.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    ATGGCATTAA AGAGCTTGAA AACAGAAGAT TACGTTCAGC AGTTATCCTG TGTCAAGGAC 
    ACCATAGTCC TCCACAACGA ACTGAAGAAA CATGTGGATG GTGGCTTGGG TCCTTATGGT
    CGCACAATGT GTGATCGTAT CATCAGAGCC TGCAACCAGC GGCTGGGCAA CGGCTCTGTG
    GATGCTGGCC TTCAGGAGCG GATAGTGGAG CTGGTGGAAC TGGCTGTCCA AGGGTTTCAA
    CTGCTTGAAG AGTCCGGCAT GCAGAGCACC CCCTTTTATC TGGAGAAAAT AGTCTTTCAC
    ATCCTACAGA AAGTCGCAAG TCTTGGAGCT CATGGTCCTG CTTGTCGACT GGGTCAGCTC
    GTGTATAGAA GACTGAAATG TTTGTCTGCA GAGATTGACG ACTTTAATGT CCTGGTGCGG
    AATTGCTTTG CAGTCTTGTG GAACAGTCTC CTCTCTAACA AACCGAGTGC TTCCACCCTC
    CCTCCACGAG ACAGACTCCA CTGTCAGCTG CAAGCTGTAA TATTCAGACT CCTTGAACAG
    GGCTCCAGCT TGTCCTCTTC CCCATCCAAA GTTCCTCTGT TTGTTGAGGA AGCAGTAGTG
    GAGTACGAAC GATCATGTGG AGGTTTTACT CAAGACGATG TGAGTTTTCT GACCTCTGAA
    TTGCGTGAGC TGTTCTCTGG TGCTTTATTC AATCCTCAGG ACTCTGCATC AACTTCTTCA
    CTGCTGGCTG TGTGGTGTGA GGTGGCATTT AAAGTGTCTA AACTGCTCTG CAAAAGTGGC
    TTTTCACCTG AGGCTGTTGA ACTCTTGCGA GGCACTCTGA AGGAGGTTAA TGGCCACGTT
    GGCCTGCGTT CGGCTCTGGG TCTGGCAGAC CGGGCAGTGC AGATGCAGTG TGTGTTAAGT
    TCTGGAGGTG AATGCAGTAA GAGCTTTACT GAATGTGCCC GTGCTCTCCG GATGTTGCCT
    TGTGCCATGT CCAGCTCAGA GTCTCATGCC CTGTTAGAGG CTTGTCAGCT GGTGGTTTGG
    ACGTTGGAAG CTAGTCAGTG TAAAGGAATG GATGTGACCA CTTTGCTGGC TTGTTTTTCC
    TTTTTGGAAG AATATCAGGA ATTTCTCCCT ACACAGCAAA AGAACTCGTT TTCGCTGCAG
    GTGCAATACT CTCTTTGTTT CAGTTTTTAC CAGGGCTTCA TCTGCACGTA TGATAGTCTT
    CATGCATCTC AGGTGTCTGT GGGCGAGACC TTGGACAGAG TGCTATTGTT CTGCCGGTCG
    ACCGCAGGAC GTATGCTTGC TGAACTGCGG ACACTCAATA ATGACAACTT GCTGCTTAAA
    GCAGTGTGTG CAGTGAATAA TGTGGTGTAC GAGCTGTTTA ACAGGAAGCT GTATGAAGGA
    GCCTTTGGAT TGTCGATGAT TGTGTGTCAG GAGCTCTGTA AGGACTGCCC GCCCTCTCTT
    CCTGTGGATC GGGTCAACCG CTGTTTCATG CTGACAGTGC AGTGTAGTCG CCGCGCAGGC
    CATCTGGAGA AGGCTCTGGA TTGGATTGTT CGCTGGATTC AGGCTTTGGG CTCACAGATT
    TTGGATCATC TCACTGAACC TGTGTCGATG TGGGTCAAAA CTAAGTGTGA TGCCGCCAGA
    GCAGGCGAGG ATGACACACG CCTGAGGACT CTGAGGGACG GGTTAGGAGA ATCCTTAGTG
    GATGAGGAGG TTATGGTGTG TTTGTTAGAG GAGGAGTTGC GTGTATATAA GGAGCAGACT
    GGAGACACAG CACAGGAACG CTACAACACA CTCTGCGACC TCTTGGAAAT CTGCCATGAA
    GAAACCCCAC GCACACTAAG AAGAGCTACG TATCTGTGTG AGATGGCGCA GGTTGTGTGC
    TATCAGGACT TCAGCCAGCA GACTGACTGC TCAGCAGTTG ATTTTACCCA CGAGGCTTTG
    CGGTTGCTGG AAGCAGAACC AGAGACGGCA GAGAATACTG ATAGGCTGAA GGATGAGAAA
    GCCCACGCTT CCCTGTGGCT CTATATCTGC ACTCTTGAGT CTAACCTGCA GGAGGCTGTG
    GACACAGAGA AGCGTTTGTG TGCAGTGCAG GAGGAGAATA AAAGAGATGT GAATATGGAT
    CCTGTTCCCA CAAATGATCT GGAGTACGAG GACAAACAGA AATCACAAGA GAGCCAGTTA
    GTCTACGATG GACTACGCTT CAACCTGCTG GCTCACAGCA AGCAAAGTGA ACCTCTAGAT
    AGATGTTTGT CTCTATGGCG GAGTCTCCTC AGAGGGTCTG TGCCTGCAGT TAGAGACCCT
    AAACTCACAG CTTCTTCCAT GACTTTGATG GCTGCTCTAT ACACACTTAT GGGCAAACAC
    CTGCAAGCTC TGGAGGGATA TCAGCTTGCT GCCACCCTTT TCCATGTACT TGGTGATGGT
    CAGAATAGTG CCAATGCCTT CTGCCACTCT ACCAGGATCT TGCTTTACCT GGGTTCGCCG
    CAGCTTGCAC AGGTGGACTT AGAAAAAGCA GAAAAGAGCT TGTCATCCGT CACCATCTCT
    GAGGGCACAT CTATTATCTC CATGCTGGCC ATGTTGCAAA AAGCACAGCT ACACTATGCT
    CTAGGACAGG TGGAGAGTGG CGTGTCCTGC CTGATGGAGG TGATAAAGGA ATCTGCTCAG
    CATCATTCTA AGAGCTGGTA CCTCCTCAGG GCTCGAGCAC TGCAGACAGC CAGTGAATAC
    TTGAGTCTGG ACACACTGGC TTTGAACTCA CAGCTACGCC AGATCATCAT GCAGCAGGGT
    CTAAAGACTC CAGACACTGC TCAATATGAA GGGTTGAAGC TGTTGTGTAG CCTGGTGATG
    ATGTTATTGG GCAACGGGTT TTACGGAGCG CCTGGACCAA ACACTGACAC TCGCTTTGTG
    GATAAAGGAG ACAGTGTTGT GTTTAAGTGG TTGTTGCTGA GTGAAGTGCT GGTGTGCTCG
    GAGAGAATGG TGATCACCAG GAGCAGCAGC GGAGCCGTTC ATGAAGCTAA AGCTCAGTGT
    CTAGAGGCTC TCAAACTGGC CACCAAACTG CAGACACTCA GCCACTGTGC TGAGCTGTTG
    GTGCTGAAGG CAGAGCTGGA GCTGATGAAG GGTGCTACTG AAGCTAGTAG TTTAGACCTG
    GAGCAAGTTA GACATTTACT TGATCTCTGC ACAGATTTTG GCCAGGGACG ACAACAGAAA
    TCTGAAGTGA AAATCAAACC ACGCAAAGGT CGTCCCGCTG CCCCTTCTTC CTCTGGTCAC
    ATCTCTGAGG AAGAGGATGA TCTGAGTGGG ATCCTCAGCT CTCGTGCCCT TCCGAAGGAG
    CCTGTGGAGA ACATGGCTAG ATTAGGAAGC CAAGGAGCTT CTCCCCCTCT AAAGCCCAAA
    CGCCAGCATG GCCTCTCCTG TCTGACCCAT GAAGAGACCT GCTCTTGTCT CTGCTGCACT
    GAGCTCAGCG TGGCTCGAGT CAGCATCCAC TGGGCACTAT TACAGGCAGA TCTCCAGACA
    GATCCCGAAC ACTCTCTCAG ACTACGCCAG TCTGCCAGGA AACGCTGCCG CTATGTTGTA
    ACTAAACTTC AGAGAAGTTT GGCAGCACTT GTACACTTCA AAAAATCAAC TAATGTCTGC
    TTGTCACTGC TTCAGGCTGA ACAGGGTAAA GTGCATCTGG GTGCTGTGCT TCAACTGCTC
    AGAACTGGAG ACAAAGGGAA AGCTGCCATC TTGTGGGAGG AGATAGAAGC AGGATTAGAG
    GCAGTGACAC CTAAAGGAGC ACTGACACCA GAACTTGGGC CTATCAGAGC TGCTCTACTG
    GGGGCTAAAG CCGTAGCCTG CTGCTTGGCG TTGGCCATGA AGAAACAGTG TATACCCGAA
    GAGCTGTTTT CTTCATTGTG GCACTGGAAT GCAACAAAGG TTAAACCGCA ATTAAAATCT
    AAGCATCGAT CCAAGAGTCC GGACGCAGCT CCTCTAGAGC CAGGTGTTAA ACATAACCCT
    AAAGTTAAGA GTGGACTTGA GGAGAATGAA AGCAGAAAAA AAGAGACCAC TGTCATTTCG
    AAGAAAGCTA AAGACTCTGT GCCCAAGATT ACTATTACAA AATCCTCCAT GGTTTTTAAA
    ACGCCCAAAG CAACGAGAAC AACCCGGCCC AAATCTGTCT CCACTAGCAC TGGCATCAGT
    GACCTGAGAG CATTTGACTT CAATAATGAG GTGCCTGACA TTTCAGTCAA CTTCACACCA
    TCATTACACC GATCTACAAA CCAAAGTGCG ACTGCAAAGT CAAAAGCGTC CTCAAAGGGG
    TCGTTCCAGG TTTACAAAGA TTCTTCTCCT GCTGCAGAGA AAGCTGTAGT TGTCCCGGCT
    GCTCCTAAAC GAACAAAGCG TTCTCGCTTC AAGGTTGAGT TCAGCGATGA AAGCGACACC
    GAAGCTGCTC CTCTTGCTGT AATGGAAAAG TCAGAGAAAA AGAAAAACAC CTCTAAAACA
    TCACGCACCT TGAAACCAAC CCTTAACACC ATCCCTAGTG ACACCAAACT TTTGAGCTCC
    ACAGTAGATC CAGAACCCTC CAGACGAACA CGGACCACTA AGAAAAGCAC AGCTCTTCCC
    TCCATTGGTG GCCTCTCTTC TGAGGAAGAA CTTGGAGTAT CTTCACAGAC ACGTACGCGT
    AGAGGACGAC CAAAGAAGAG CACCAGCTCA GCAGACACAG AGGAGCCAGA GAGGATGAGG
    ATGATTAAGG AGGAAGAACA GAGGGTTCTT TTGAATGTCA GCATGGAGGA GCTCAGAGGC
    TCTGACACCG AGACAAAAGA CACTGGAAGT CCAGATGCTG ACTGTGAGGT CTTGAGGAGA
    GATCTAGGTG CAGATTTTGG GCGAGAGTGT TTCAGTGAGC TCAGGAGAGC TGGACAGAAT
    GGATCAGGAT CTCACACAGC TCTGCCACAT GCAACTACAT CTCCTGACTT GTCAGTAGAG
    ACGGTCCAGT CATACCTGCG CTCCTCATGG CTCCTCCTGC ACCACTTCCC GTTTCCTTCT
    CTTTATCCTC ACATCTGCTC ATTACTCGCT CAGTCATTCG GTCAAACGGA CCCCATCATC
    ACAGCAATGC TGCATGCACA GGCACTGGGA GTGTCCACTC GCCACCATAT GACCCGTCAC
    GTGGTTAACC AGTTCAGAAA GTTGAAGAAA TCCTGTAACG ATGTAGCAGA AGGTCTTGGA
    GCTCTGAGTC TGGAGGAAAC ATCTGGAGCA ACGCAGTCAA AGAAACTCTC TGCGTTGGAG
    CAGATCTTTT CATTCACAGC ATCAAACCCA ATGCAGTTTC CACAGACACA CTGTCAGCAG
    TTCACACAGC AGCTGGAAGA TCTACCTGCA GGAGTGACTG TATGCTTGCT GTCACTGACG
    GGGGTGCATC CTGATGAAAT TGGCAACACT ATTCTTCTGA CCCGACTGGA GCGCGGCTCT
    ACCCCAATCA CAGTTCGAAT CCCCACTGCA GACAGAGAGC AATCTGTTGC TGGCCTGTTG
    GAAGAAATGG ATGGAGTCTT GAAAGGACAA AAGGAAGTGA GCACAGTGGC TGAGAAATCA
    CAGTGGTGGG ACGGAAGGAA GGCTTTAGAT GCCCGTGTGG AGAAAATGCT GGAGGAGATG
    GAAGAGGCTT TAGGAGTGTG GCGAACTCTT CTTCTGCCTT TGACCTCTGA TCCTAAGCTT
    GATGTACAGG TAAAGAGCCT CCAAAAGGCT CTTAAATCAA CAAAGATCAC ACAGGATACT
    CTCAAGGTGA TTCTTTCAGC CTCTCCACTG CTGTCCCTGC CAGACCTGCA GTGTCTTCTG
    GAAGGAATGA ATGTGCAGGA CAAGGACCTG CTGAAGCTCC TTCAGAGAGG CGTGGCTGAA
    CTGAGAGGGA GGGAGGAGCC ACAGGGACAC ACTGTTCTGA TTTTAGACAA GTACTTGCAG
    AGGTTGCCGT GGGAGAACAT TGCTTGTCTG AAGTCTCAGT CGGTCACTCG AATGCCCTCA
    TTACATGCGG TGCTTGGACA CAGTCATCTG AGAGAGGTGG ATACAAGTTG TGTTCTGTCT
    TGTGGTGTGA ACCCTAAGCA GGTTTACTAT GTGCTAAACC CAGATGGAAA CCTGCCCGAC
    ACTGAGAATC GCTTCAAAGA GTGGTTTACA GGTGAACGAA CATGGCAGGG GGTTTGTGGA
    AGGGCTCCTG ATCCGGAGAA ACTACAAGAG GCTGTGACCA CTAAAGATCT CTACATATAC
    ATCGGACATG GTGCAGGAGC CCGCTTTCTG GATGCGCAGA GAGTTTTGAA GGTGCCTGTG
    AGGGCCGCAG CTCTTCTGTT CGGCTGCAGT AGTGCTGCTC TCAGTGTGCT TGGTCAGCAG
    GAGGGAACGG GCATCATCCT CAGCTACCTG ACCGCTGGAT GTCCAATGGT GCTGGGTAAC
    CTGTGGGATG TGACAGATCG TGACTTGGAT CGATTCACAT CGGCTCTGCT CCAGTCTTGG
    CTCTCTGCTG GTTCTGGCTC TTCACTTCTT CAGCATTTGG CGAAATCACG CAGCGCAACT
    CATTTAAAAC ACATAATCGG TGCTGCGCCC ATTGCGTATG GTTTACCGGT GCATATACAC
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021329799.1
    CDS222..6764
    Translation

    Target ORF information:

    RefSeq Version XM_021474124.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio extra spindle pole bodies like 1, separase (espl1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021474124.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    ATGGCATTAA AGAGCTTGAA AACAGAAGAT TACGTTCAGC AGTTATCCTG TGTCAAGGAC 
    ACCATAGTCC TCCACAACGA ACTGAAGAAA CATGTGGATG GTGGCTTGGG TCCTTATGGT
    CGCACAATGT GTGATCGTAT CATCAGAGCC TGCAACCAGC GGCTGGGCAA CGGCTCTGTG
    GATGCTGGCC TTCAGGAGCG GATAGTGGAG CTGGTGGAAC TGGCTGTCCA AGGGTTTCAA
    CTGCTTGAAG AGTCCGGCAT GCAGAGCACC CCCTTTTATC TGGAGAAAAT AGTCTTTCAC
    ATCCTACAGA AAGTCGCAAG TCTTGGAGCT CATGGTCCTG CTTGTCGACT GGGTCAGCTC
    GTGTATAGAA GACTGAAATG TTTGTCTGCA GAGATTGACG ACTTTAATGT CCTGGTGCGG
    AATTGCTTTG CAGTCTTGTG GAACAGTCTC CTCTCTAACA AACCGAGTGC TTCCACCCTC
    CCTCCACGAG ACAGACTCCA CTGTCAGCTG CAAGCTGTAA TATTCAGACT CCTTGAACAG
    GGCTCCAGCT TGTCCTCTTC CCCATCCAAA GTTCCTCTGT TTGTTGAGGA AGCAGTAGTG
    GAGTACGAAC GATCATGTGG AGGTTTTACT CAAGACGATG TGAGTTTTCT GACCTCTGAA
    TTGCGTGAGC TGTTCTCTGG TGCTTTATTC AATCCTCAGG ACTCTGCATC AACTTCTTCA
    CTGCTGGCTG TGTGGTGTGA GGTGGCATTT AAAGTGTCTA AACTGCTCTG CAAAAGTGGC
    TTTTCACCTG AGGCTGTTGA ACTCTTGCGA GGCACTCTGA AGGAGGTTAA TGGCCACGTT
    GGCCTGCGTT CGGCTCTGGG TCTGGCAGAC CGGGCAGTGC AGATGCAGTG TGTGTTAAGT
    TCTGGAGGTG AATGCAGTAA GAGCTTTACT GAATGTGCCC GTGCTCTCCG GATGTTGCCT
    TGTGCCATGT CCAGCTCAGA GTCTCATGCC CTGTTAGAGG CTTGTCAGCT GGTGGTTTGG
    ACGTTGGAAG CTAGTCAGTG TAAAGGAATG GATGTGACCA CTTTGCTGGC TTGTTTTTCC
    TTTTTGGAAG AATATCAGGA ATTTCTCCCT ACACAGCAAA AGAACTCGTT TTCGCTGCAG
    GTGCAATACT CTCTTTGTTT CAGTTTTTAC CAGGGCTTCA TCTGCACGTA TGATAGTCTT
    CATGCATCTC AGGTGTCTGT GGGCGAGACC TTGGACAGAG TGCTATTGTT CTGCCGGTCG
    ACCGCAGGAC GTATGCTTGC TGAACTGCGG ACACTCAATA ATGACAACTT GCTGCTTAAA
    GCAGTGTGTG CAGTGAATAA TGTGGTGTAC GAGCTGTTTA ACAGGAAGCT GTATGAAGGA
    GCCTTTGGAT TGTCGATGAT TGTGTGTCAG GAGCTCTGTA AGGACTGCCC GCCCTCTCTT
    CCTGTGGATC GGGTCAACCG CTGTTTCATG CTGACAGTGC AGTGTAGTCG CCGCGCAGGC
    CATCTGGAGA AGGCTCTGGA TTGGATTGTT CGCTGGATTC AGGCTTTGGG CTCACAGATT
    TTGGATCATC TCACTGAACC TGTGTCGATG TGGGTCAAAA CTAAGTGTGA TGCCGCCAGA
    GCAGGCGAGG ATGACACACG CCTGAGGACT CTGAGGGACG GGTTAGGAGA ATCCTTAGTG
    GATGAGGAGG TTATGGTGTG TTTGTTAGAG GAGGAGTTGC GTGTATATAA GGAGCAGACT
    GGAGACACAG CACAGGAACG CTACAACACA CTCTGCGACC TCTTGGAAAT CTGCCATGAA
    GAAACCCCAC GCACACTAAG AAGAGCTACG TATCTGTGTG AGATGGCGCA GGTTGTGTGC
    TATCAGGACT TCAGCCAGCA GACTGACTGC TCAGCAGTTG ATTTTACCCA CGAGGCTTTG
    CGGTTGCTGG AAGCAGAACC AGAGACGGCA GAGAATACTG ATAGGCTGAA GGATGAGAAA
    GCCCACGCTT CCCTGTGGCT CTATATCTGC ACTCTTGAGT CTAACCTGCA GGAGGCTGTG
    GACACAGAGA AGCGTTTGTG TGCAGTGCAG GAGGAGAATA AAAGAGATGT GAATATGGAT
    CCTGTTCCCA CAAATGATCT GGAGTACGAG GACAAACAGA AATCACAAGA GAGCCAGTTA
    GTCTACGATG GACTACGCTT CAACCTGCTG GCTCACAGCA AGCAAAGTGA ACCTCTAGAT
    AGATGTTTGT CTCTATGGCG GAGTCTCCTC AGAGGGTCTG TGCCTGCAGT TAGAGACCCT
    AAACTCACAG CTTCTTCCAT GACTTTGATG GCTGCTCTAT ACACACTTAT GGGCAAACAC
    CTGCAAGCTC TGGAGGGATA TCAGCTTGCT GCCACCCTTT TCCATGTACT TGGTGATGGT
    CAGAATAGTG CCAATGCCTT CTGCCACTCT ACCAGGATCT TGCTTTACCT GGGTTCGCCG
    CAGCTTGCAC AGGTGGACTT AGAAAAAGCA GAAAAGAGCT TGTCATCCGT CACCATCTCT
    GAGGGCACAT CTATTATCTC CATGCTGGCC ATGTTGCAAA AAGCACAGCT ACACTATGCT
    CTAGGACAGG TGGAGAGTGG CGTGTCCTGC CTGATGGAGG TGATAAAGGA ATCTGCTCAG
    CATCATTCTA AGAGCTGGTA CCTCCTCAGG GCTCGAGCAC TGCAGACAGC CAGTGAATAC
    TTGAGTCTGG ACACACTGGC TTTGAACTCA CAGCTACGCC AGATCATCAT GCAGCAGGGT
    CTAAAGACTC CAGACACTGC TCAATATGAA GGGTTGAAGC TGTTGTGTAG CCTGGTGATG
    ATGTTATTGG GCAACGGGTT TTACGGAGCG CCTGGACCAA ACACTGACAC TCGCTTTGTG
    GATAAAGGAG ACAGTGTTGT GTTTAAGTGG TTGTTGCTGA GTGAAGTGCT GGTGTGCTCG
    GAGAGAATGG TGATCACCAG GAGCAGCAGC GGAGCCGTTC ATGAAGCTAA AGCTCAGTGT
    CTAGAGGCTC TCAAACTGGC CACCAAACTG CAGACACTCA GCCACTGTGC TGAGCTGTTG
    GTGCTGAAGG CAGAGCTGGA GCTGATGAAG GGTGCTACTG AAGCTAGTAG TTTAGACCTG
    GAGCAAGTTA GACATTTACT TGATCTCTGC ACAGATTTTG GCCAGGGACG ACAACAGAAA
    TCTGAAGTGA AAATCAAACC ACGCAAAGGT CGTCCCGCTG CCCCTTCTTC CTCTGGTCAC
    ATCTCTGAGG AAGAGGATGA TCTGAGTGGG ATCCTCAGCT CTCGTGCCCT TCCGAAGGAG
    CCTGTGGAGA ACATGGCTAG ATTAGGAAGC CAAGGAGCTT CTCCCCCTCT AAAGCCCAAA
    CGCCAGCATG GCCTCTCCTG TCTGACCCAT GAAGAGACCT GCTCTTGTCT CTGCTGCACT
    GAGCTCAGCG TGGCTCGAGT CAGCATCCAC TGGGCACTAT TACAGGCAGA TCTCCAGACA
    GATCCCGAAC ACTCTCTCAG ACTACGCCAG TCTGCCAGGA AACGCTGCCG CTATGTTGTA
    ACTAAACTTC AGAGAAGTTT GGCAGCACTT GTACACTTCA AAAAATCAAC TAATGTCTGC
    TTGTCACTGC TTCAGGCTGA ACAGGGTAAA GTGCATCTGG GTGCTGTGCT TCAACTGCTC
    AGAACTGGAG ACAAAGGGAA AGCTGCCATC TTGTGGGAGG AGATAGAAGC AGGATTAGAG
    GCAGTGACAC CTAAAGGAGC ACTGACACCA GAACTTGGGC CTATCAGAGC TGCTCTACTG
    GGGGCTAAAG CCGTAGCCTG CTGCTTGGCG TTGGCCATGA AGAAACAGTG TATACCCGAA
    GAGCTGTTTT CTTCATTGTG GCACTGGAAT GCAACAAAGG TTAAACCGCA ATTAAAATCT
    AAGCATCGAT CCAAGAGTCC GGACGCAGCT CCTCTAGAGC CAGGTGTTAA ACATAACCCT
    AAAGTTAAGA GTGGACTTGA GGAGAATGAA AGCAGAAAAA AAGAGACCAC TGTCATTTCG
    AAGAAAGCTA AAGACTCTGT GCCCAAGATT ACTATTACAA AATCCTCCAT GGTTTTTAAA
    ACGCCCAAAG CAACGAGAAC AACCCGGCCC AAATCTGTCT CCACTAGCAC TGGCATCAGT
    GACCTGAGAG CATTTGACTT CAATAATGAG GTGCCTGACA TTTCAGTCAA CTTCACACCA
    TCATTACACC GATCTACAAA CCAAAGTGCG ACTGCAAAGT CAAAAGCGTC CTCAAAGGGG
    TCGTTCCAGG TTTACAAAGA TTCTTCTCCT GCTGCAGAGA AAGCTGTAGT TGTCCCGGCT
    GCTCCTAAAC GAACAAAGCG TTCTCGCTTC AAGGTTGAGT TCAGCGATGA AAGCGACACC
    GAAGCTGCTC CTCTTGCTGT AATGGAAAAG TCAGAGAAAA AGAAAAACAC CTCTAAAACA
    TCACGCACCT TGAAACCAAC CCTTAACACC ATCCCTAGTG ACACCAAACT TTTGAGCTCC
    ACAGTAGATC CAGAACCCTC CAGACGAACA CGGACCACTA AGAAAAGCAC AGCTCTTCCC
    TCCATTGGTG GCCTCTCTTC TGAGGAAGAA CTTGGAGTAT CTTCACAGAC ACGTACGCGT
    AGAGGACGAC CAAAGAAGAG CACCAGCTCA GCAGACACAG AGGAGCCAGA GAGGATGAGG
    ATGATTAAGG AGGAAGAACA GAGGGTTCTT TTGAATGTCA GCATGGAGGA GCTCAGAGGC
    TCTGACACCG AGACAAAAGA CACTGGAAGT CCAGATGCTG ACTGTGAGGT CTTGAGGAGA
    GATCTAGGTG CAGATTTTGG GCGAGAGTGT TTCAGTGAGC TCAGGAGAGC TGGACAGAAT
    GGATCAGGAT CTCACACAGC TCTGCCACAT GCAACTACAT CTCCTGACTT GTCAGTAGAG
    ACGGTCCAGT CATACCTGCG CTCCTCATGG CTCCTCCTGC ACCACTTCCC GTTTCCTTCT
    CTTTATCCTC ACATCTGCTC ATTACTCGCT CAGTCATTCG GTCAAACGGA CCCCATCATC
    ACAGCAATGC TGCATGCACA GGCACTGGGA GTGTCCACTC GCCACCATAT GACCCGTCAC
    GTGGTTAACC AGTTCAGAAA GTTGAAGAAA TCCTGTAACG ATGTAGCAGA AGGTCTTGGA
    GCTCTGAGTC TGGAGGAAAC ATCTGGAGCA ACGCAGTCAA AGAAACTCTC TGCGTTGGAG
    CAGATCTTTT CATTCACAGC ATCAAACCCA ATGCAGTTTC CACAGACACA CTGTCAGCAG
    TTCACACAGC AGCTGGAAGA TCTACCTGCA GGAGTGACTG TATGCTTGCT GTCACTGACG
    GGGGTGCATC CTGATGAAAT TGGCAACACT ATTCTTCTGA CCCGACTGGA GCGCGGCTCT
    ACCCCAATCA CAGTTCGAAT CCCCACTGCA GACAGAGAGC AATCTGTTGC TGGCCTGTTG
    GAAGAAATGG ATGGAGTCTT GAAAGGACAA AAGGAAGTGA GCACAGTGGC TGAGAAATCA
    CAGTGGTGGG ACGGAAGGAA GGCTTTAGAT GCCCGTGTGG AGAAAATGCT GGAGGAGATG
    GAAGAGGCTT TAGGAGTGTG GCGAACTCTT CTTCTGCCTT TGACCTCTGA TCCTAAGCTT
    GATGTACAGG TAAAGAGCCT CCAAAAGGCT CTTAAATCAA CAAAGATCAC ACAGGATACT
    CTCAAGGTGA TTCTTTCAGC CTCTCCACTG CTGTCCCTGC CAGACCTGCA GTGTCTTCTG
    GAAGGAATGA ATGTGCAGGA CAAGGACCTG CTGAAGCTCC TTCAGAGAGG CGTGGCTGAA
    CTGAGAGGGA GGGAGGAGCC ACAGGGACAC ACTGTTCTGA TTTTAGACAA GTACTTGCAG
    AGGTTGCCGT GGGAGAACAT TGCTTGTCTG AAGTCTCAGT CGGTCACTCG AATGCCCTCA
    TTACATGCGG TGCTTGGACA CAGTCATCTG AGAGAGGTGG ATACAAGTTG TGTTCTGTCT
    TGTGGTGTGA ACCCTAAGCA GGTTTACTAT GTGCTAAACC CAGATGGAAA CCTGCCCGAC
    ACTGAGAATC GCTTCAAAGA GTGGTTTACA GGTGAACGAA CATGGCAGGG GGTTTGTGGA
    AGGGCTCCTG ATCCGGAGAA ACTACAAGAG GCTGTGACCA CTAAAGATCT CTACATATAC
    ATCGGACATG GTGCAGGAGC CCGCTTTCTG GATGCGCAGA GAGTTTTGAA GGTGCCTGTG
    AGGGCCGCAG CTCTTCTGTT CGGCTGCAGT AGTGCTGCTC TCAGTGTGCT TGGTCAGCAG
    GAGGGAACGG GCATCATCCT CAGCTACCTG ACCGCTGGAT GTCCAATGGT GCTGGGTAAC
    CTGTGGGATG TGACAGATCG TGACTTGGAT CGATTCACAT CGGCTCTGCT CCAGTCTTGG
    CTCTCTGCTG GTTCTGGCTC TTCACTTCTT CAGCATTTGG CGAAATCACG CAGCGCAACT
    CATTTAAAAC ACATAATCGG TGCTGCGCCC ATTGCGTATG GTTTACCGGT GCATATACAC
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa12112
    Clone ID Related Accession (Same CDS sequence) XM_021477220.1 , XM_001337833.4 , XM_001337833.5 , XM_017356662.1
    Accession Version XM_021477220.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6549bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product separin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    ATGGCATTAA AGAGCTTGAA AACAGAAGAT TACGTTCAGC AGTTATCCTG TGTCAAGGAC 
    ACCATAGTCC TCCACAACGA ACTGAAGAAA CATGTGGATG GTGGCTTGGG TCCTTATGGT
    CGCACAATGT GTGATCGTAT CATCAGAGCC TGCAACCAGC GGCTGGGCAA CGGCGCTGTG
    GATGCTGGCC TTCAGGAGCG GATAGTGGAG CTGGTGGAAC TGGCTGTCCA AGGGTTTCAA
    CTGCTTGAAG AGTCCGGCAT GCAGAGCACC CCCTTTTATC TGGAGAAAAT AGTCTTTCAC
    ATCCTACAGA AAGTCGCAAG CCTTGGAGCT CATGGTCCTG CTTGTCGACT GGGTCAGCTC
    GTGTATAGAA GACTGAAATG TTTGTCTGCA GAGATTGATG ACTTTAATGT CCTGGTGCGG
    AATTGCTTTG CAGTCTTGTG GAACAGTCTC CTCTCTAACA AACCGAGTGC TTCCACCCTC
    CCTCCACGAG ACAGACTCCA CTGTCAGCTG CAAGCTTTAA CATTCAGACT CCTTGAACAG
    GGCTCCAGCT CGTCCTCTTC CCCATCCAAA GTTCCTCTGT TCGTGGAGGA AGCAGTAGTG
    GAGTACGAAC GATCATGTGG AGGTTTTACT CAAGACGATG TGAGTTTTCT GACCTCTGAA
    TTGCGTGAGC TGTTCTCTGG TGCTTTCATC AATCCTCAGG ACTCTGCATC AACTTCTTCA
    CTGCTGGCTG TGTGGTGTGA GGTGGCATTT AAAGTGTCTA AACTGCTCTG CAAAAGTAGC
    TTTTCACCTG AGGCTGTTGA ACTCTTGCGA GGCACTCTGA AGGAGGTTAA TGGCCACGTT
    GGCCTGCGTT CGGCTCTGGG TCTGGCAGAC CGGGCAGTGC AGATGCAGTG TGTGTTAAGT
    TCTGGAGGTG AATGCAGTAA GAGCTTCACT GAATGTGCCC GTGCTCTCCG GATGTTGCCT
    TGTGCCATGT CCAGCTCAGA GTCTCATGCC CTGTTAGAGG CTTGTCAGCT GGTGGTTTGG
    ACGTTGGAAG CCAGTCAGTG TAAAGGAATG GATGTGACCA CTTTGCTGGC TTGTTTTTCC
    TTTTTGGAAG AATATCAGGA ATTTCTGCTT GCACAGCAAA AGAACTCGTT TTCGCTGCAG
    GTCCAATACT CTCTTTGTTT CAGTTTTTAC CAGGGCTTCA TCTGCACATA TGATAGTCTT
    CATGCTTCTC AGGTGTCTGT GGGCGAGACC TTGGACAGAG TGCTATTGTT CTGCCGGTCG
    ACCGCAGGAC GTATGCTTGC TGAACTGCGG ACACTCAATA ATGACAACTT GCTGCTTAAA
    GCAGTGTGTG CAGTGAATAA TGTGGTGTAC GAGCTGTTTA ACAGGAAGCT GTATGAAGGA
    GCCTTTGGAT TGTCGATGAT TGTGTGTCAG GAGCTCTGTA AGGACTGCCC GCCCTCTCTT
    CCTATGGATC GGGTCAACCG CTGTTTCATG CTGACAGTGC AGTGTAGTCG CCGCGCAGGC
    CATCTGGAGC AGGCTCTGGA CTGGATTGTT CGCTGGATTC AGGCTTTGGG CTCACAGATT
    TTGGATCATC TCACTGAACC TGTGTCGATG TGGGTCAAAA CTAAGTGTGA TGCCGCCAGA
    GCAGGCGAGG ATGACACACG CCTGAGGACT CTGAGGGACG GGTTAGGAGA ATCCTTAGTG
    GATGAGGAGG TTATGATGTG TTTGTTAGAG GAGGAGTTGC GTGTATATAA GGAGCAGACT
    GGAGACACAG CACAGGAACG CTACAACACA CTCTGCGACC TCTTGGAAAT CTGCCATGAA
    GAAACCCCAC ACACACTAAG AAGAGCTACG TATCTGTGTG AGATGGCACA GGTTGTGTGC
    TATCAGGACT TCAGCCAGCA GACTGACTGC TCAGCAGTTG ATTTTACCCA CGAGGCTTTG
    CGGTTGCTGG AAGCAGAACC AGAGACGGCA GAGAATACTG ATAGGCTGAA GGATGAGAAA
    GCCCACGCTT CCCTGTGGCT CTATATCTGC ACTCTTGAGT CTAACCTGCA GGAGGCTGTG
    GACACAGAGA AGCGTTTGTG TGCAGTGCAG GAGGAGAATA AAAGAGATGT GAATATGGAT
    CCTGTTCCCA CAAATGATCT GGAGTACGAG GACAAACAGA AATCACAAGA GAGCCAGTTA
    GTCTACGATG GACTACGCTT CAACCTGCTG GCTCACAGCA AGCAAAGTGA ACCTCTAGAT
    AAATGTTTGT CTCTATGGCG GAGTCTCCTC AAGGGGGCTG TGCCTGCAGT TAGAGACCCT
    AAACTCACAG CTTCTTCCAT GACTTTGATG GCTGCCCTAT ACACACTTAT GGGCAAGCAC
    CTGCAAGCTC TGGAGGGATA TCAGCTTGCT GCCACCCTTT TCCATGTACT TGGTGATGGC
    CAGAGTAGTG CCAATGCCTT CTGCCACTCT ACCAGGATCT TGCTTTACCT GGGTTCACCG
    CAGCTTGCAC AGGTGGACTT AGAAAAAGCA GAAAAGAGCT TGTCATCTGT CACCATCTCT
    GAGGGCACAT CTATTATCTC CATGCTGGCC ATGTTGCAAA AAGCACAGCT ACACTATGCT
    CTAGGACAGG TGGAGAGTGG CGTGTCCTGC CTGATGGAGG TGATAAAGGA ATCTGCTCAG
    CATCATTCTA AGAGCTGGTA CCTGCTCAGG GCTCGAGCAC TGCAGACAGC CAGTGAATAC
    TTGAGTCTGG ACACACTGGC TTTGAACTCA CAGCTACGCC AGAGCATCAT GCAGCAGGGT
    CTAAAGACTC CAGACACTGC TCAATATGAA GGGTTGAAGC TGTTGTGTAG CCTGGTGATG
    ATGTTATTGG GCAACGGGTT TTACGGAGCG CCTGGACCAA ACACTGACAC TCGCTTTGTG
    GATAAAGGAG ACAGTGTTGT GTTTAAGTGG CTGCTGCTGA GTGAAGTGCT GGTGTGCTCG
    GAGAGAATGG TGATCACCAG GAGCAGCAGC GGAGCCGTTC ATGAAGCTAA AGCTCAGTGT
    CTAGAGGCTC TCAAACTGGC CACCAAACTA CAGACACTCA GCCACTGTGC TGAGCTGTTG
    GTGCTGAAGG CAGAGCTGGA GCTGATGAAG GGTGCAACTG AAGCTAGTAG TTTAGACCTA
    GAGCAAGTTA GACATTTACT TGATCTCTGC ACAGATTTTG GCCAGGGACG ACAACAGAAA
    TCTGAAGTGA AAATCAAACC ACGCAAAGGT CGTCCCGCTG CCCCTTCTTC CTCTGGTCAC
    ATCTCTGAGG AAGAGGATGA TCTGAGTGGG ATCCTCAGCT CTCGTGCCCT TCCGAAGGAG
    CCTGTGGAGA ACATGGCTAG ATTAGGAAGC CAAGGAGCTT CTCCCCCTCT AAAGCCCAAA
    TGCCAGCATG GCCTCTCCTG TCTGACCCAT GAAGAGACCT GCTCTTGTCT CTGCTGCACT
    GAGCTCAGCG TGGCTCGAGT CAGCATCCAC TGGGCACTAT TACAGGCAGA TCTCCAGACA
    GATCCCGAAC GCTCTCTCAG ACTACGCCAG TCTGCCAGGA AACGCTGCCG CTATGTTGTA
    ACTAAACTTC AGAGAAGTTT GGCAGCACTT GTACACTCCA AAAAATCACC TAATGTCTGC
    TTGTCACTGC TTCAGGCTGA ACAGGGTAAA GTGCATCTGG GTGCTGTGCT TCAACTGCTC
    AGAACTGGAG ACAAAGGGAA AGCTGCCATC TTGTGGGAGG AGATAGAAGC AGGATTAGAG
    GCAGTGACAC CTAAAGGAGC ACTGACACCA GAACTTGGGC CTATCAGAGC TACTCTACTG
    GGGGCTAAAG CCGTAGCCTG CTGCTTGGCG TTGGCAATGA AGAAACAGTG TATACCCGAA
    GAGCTGTTTT CTTCATTGTG GCACTGGAAT CCACCAAAGG TTAAACCGCA ATTAAAATCT
    AAGCATCGAT CCAAGAGTCC GGACGCAGCT CCTCTAGAGG CAGGTGTTAA ACATAACCCT
    AAAGTTAAGA GTGGACTTGA GGAGAATGAA AGCAGAAAAA CCAAAGAGAC CACTGTCATT
    TCGAAGAAAG CTAAAGACTC TGTGCCCAAA ATTACCATCA CAAAATCCTC CATGGTTTTT
    AAAACGCCCA AAGCAACGAG AACAACCCGG CCCAAATCTG TCTCCACTAG CACTGGCATC
    AGTGACCTGA GAGCATTTGA CTTCAATAAT GAGGTGCCTG ACATTTCAGT CAACTTCACA
    CCATCATTAC ACCGATCTAC AAACCAAAGT GCGACTGCAA AGTCAAAAGC GTCCTCAAAG
    GGTTCGTTCC AGGTTTACAA AGATTCTTCT CCTGCTGCAG AGAAAGCTGT AGTTGTCCCG
    GCTGCTCCTA AACGAACAAA GCGTTCTCGC TTCAAGGTTG AGTTCAGCGA TGAAAGCGAC
    AACGATGCTG CTCCTCTTGC TGTAATGGAA AAGTCAGAGA AAAAGAAAAA CACCTTTAAA
    ACATCACGCA CCTTGAAACC AACCCTTAAC ACCATCCCTA GTGACACCAA ACTTGTGAGC
    TCCACAGTAG ATCCAGAACC CTCCAGACGA ACACGGACCA CTAAGAAAAG CACAGCTCTT
    CCCTCCATTG GTGGCCTCTC TTCTGAGGAA GAACTTGGAG TATCTTCACA GACACGTACG
    CGTAGAGGAC GACCAAAGAA GAGCACCAGC TCAGCAGACA CAGAGGAGCC AGAGAGGATG
    AGGATGATTA AGGAGGAAGA ACAGAGGGTT CTTTTGAATG TCAGCATGGA GGAGCTCAGA
    GGCTCTGACA CTGAGACAAA AGACACTGGA AGTCCAGATG CTGACTGTGA GGTCTTGAGG
    AGAGATCTAG GTGCAGATTT TGGGCGAGAG TGTTTCAGTG AGCTCAGGAG AGCTGGACAG
    AATGGATCAG GATCTCACAC AGCTCTGCCA CATGCAACTA CATCTCCTGC AGACTTGTCA
    GTAGAGACGG TCCAGTCATA CCTGCGCTCC TCATGGCTCC TCCTGCACCA CTTCCCGTTT
    CCTTCTCTTT ATCCTCACAT CTGCTCATTA CTCGCTCAGT CATTCGGTCA AACGGACCCC
    ATCATCACAG CAATGCTGCA TGCACAGGCA CTGGGAGTGT CCACTCGCCA CCATATGACC
    CGTCACGTGG TTAACCAGTT CAGAAAGTTG AAGAAATCCT GTAACGATGT AGCAGAAGGT
    CTTGGAGCTC TGAGTCTGGA GGAAACATCT GGAGCAACGC AGTCAAAGAA ACTCTCTGCG
    TTGGAGCAGA TCTTTTCATT CACAGCATCA AACCCAATGC AGTTTCCACA GACACACTGT
    CAGCAGTTCA CACAGCAGCT GGAAGATCTA CCTGCAGGAG TGACCGTATG CTTGCTGTCA
    CTGACGGGGG TGCATCCTGA TGAAATTGGC AACACTATTC TTCTGACCCG ACTGGAGCGC
    GGCTCTACCC CAATCACAGT TCGAATCCCC ACTGCAGACA GAGAGCAATC TGTTGCTGGC
    CTGTTGGAAG AAATGGATGG AGTCTTGAAA GGACAAAAGG AAGTGAGCAC AGTGGCTGAG
    AAATCACAGT GGTGGGACGG AAGGAAGGCT TTAGATGCCC GTGTGGAGAA AATGCTGGAG
    GAGATGGAAG AGGCTTTAGG AGTGTGGCGA ACTCTTTTTC TGCCTTTGAC CTCTGATCCT
    AAGCTTGATG TTCAGGTAAA GAGCCTCCAA AAGGCTCTTA AATCAACAAA GATCACACGG
    GACACTCTCA AGGTGATTCT ATCAGCGTCT CCACTGCTGT CCCTGCCAGA CCTGCAGTGT
    CTTCTGGAAG GAATGAATGT GCAGGACAAG GACCTGCTGA AGCTCCTGCA GAGAGGCGTG
    GCTGAACTGA GAGGGAGGGA GGAGCCACAG GGACACACTG TTCTGATTTT AGACAAGTAC
    TTGCAGAGGT TGCCGTGGGA GAACATTGCT TGTCTGAAGT CTCAGTCGGT CACTCGAATG
    CCCTCATTAC ACGCGGTGCT TGGACACAGT CATCTGAGAG AGGTGGATAC AAGTTGTGTT
    CTATCTTGTG GTGTGAACCC TAAGCAGGTT TACTATGTGC TAAACCCAGA TGGAAACCTG
    CCCGACACTG AGAATCGCTT CAAAGAGTGG TTTACAGGTG AACGAACATG GCAGGGGGTT
    TGTGGAAGGG CTCCTGATCC GGAGAAACTA CAAGAGGCTG TGACCACTAA AGATCTCTAC
    ATATACATCG GACATGGTGC AGGAGCCCGC TTTCTGGATG CGCAGAGAGT TTTGAAGGTG
    CCTGTGAGGA CCGCAGCTCT TCTGTTCGGC TGCAGTAGTG CTGCTCTCAG TGTGCTTGGT
    CAGCAGGAGG GAACGGGCAT CATCCTCAGC TACCTGACCG CTGGATGTCC ATTGGTGCTG
    GGTAACCTGT GGGATGTGAC AGATCGTGAC TTGGATCGAT TCACATCGGC TCTGCTCCAG
    TCTTGGCTCT CTGCTGGTTC TGGCTCTTCA CTTCTTCAGC ATTTGGCGAA ATCACGCAGC
    GCAACTCATT TAAAACACAT AATCGGTGCT GCGCCCATTG CGTATGGTTT ACCGGTGCAT
    ATACACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021332895.1
    CDS210..6758
    Translation

    Target ORF information:

    RefSeq Version XM_021477220.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio extra spindle pole bodies like 1, separase (espl1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021477220.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    ATGGCATTAA AGAGCTTGAA AACAGAAGAT TACGTTCAGC AGTTATCCTG TGTCAAGGAC 
    ACCATAGTCC TCCACAACGA ACTGAAGAAA CATGTGGATG GTGGCTTGGG TCCTTATGGT
    CGCACAATGT GTGATCGTAT CATCAGAGCC TGCAACCAGC GGCTGGGCAA CGGCGCTGTG
    GATGCTGGCC TTCAGGAGCG GATAGTGGAG CTGGTGGAAC TGGCTGTCCA AGGGTTTCAA
    CTGCTTGAAG AGTCCGGCAT GCAGAGCACC CCCTTTTATC TGGAGAAAAT AGTCTTTCAC
    ATCCTACAGA AAGTCGCAAG CCTTGGAGCT CATGGTCCTG CTTGTCGACT GGGTCAGCTC
    GTGTATAGAA GACTGAAATG TTTGTCTGCA GAGATTGATG ACTTTAATGT CCTGGTGCGG
    AATTGCTTTG CAGTCTTGTG GAACAGTCTC CTCTCTAACA AACCGAGTGC TTCCACCCTC
    CCTCCACGAG ACAGACTCCA CTGTCAGCTG CAAGCTTTAA CATTCAGACT CCTTGAACAG
    GGCTCCAGCT CGTCCTCTTC CCCATCCAAA GTTCCTCTGT TCGTGGAGGA AGCAGTAGTG
    GAGTACGAAC GATCATGTGG AGGTTTTACT CAAGACGATG TGAGTTTTCT GACCTCTGAA
    TTGCGTGAGC TGTTCTCTGG TGCTTTCATC AATCCTCAGG ACTCTGCATC AACTTCTTCA
    CTGCTGGCTG TGTGGTGTGA GGTGGCATTT AAAGTGTCTA AACTGCTCTG CAAAAGTAGC
    TTTTCACCTG AGGCTGTTGA ACTCTTGCGA GGCACTCTGA AGGAGGTTAA TGGCCACGTT
    GGCCTGCGTT CGGCTCTGGG TCTGGCAGAC CGGGCAGTGC AGATGCAGTG TGTGTTAAGT
    TCTGGAGGTG AATGCAGTAA GAGCTTCACT GAATGTGCCC GTGCTCTCCG GATGTTGCCT
    TGTGCCATGT CCAGCTCAGA GTCTCATGCC CTGTTAGAGG CTTGTCAGCT GGTGGTTTGG
    ACGTTGGAAG CCAGTCAGTG TAAAGGAATG GATGTGACCA CTTTGCTGGC TTGTTTTTCC
    TTTTTGGAAG AATATCAGGA ATTTCTGCTT GCACAGCAAA AGAACTCGTT TTCGCTGCAG
    GTCCAATACT CTCTTTGTTT CAGTTTTTAC CAGGGCTTCA TCTGCACATA TGATAGTCTT
    CATGCTTCTC AGGTGTCTGT GGGCGAGACC TTGGACAGAG TGCTATTGTT CTGCCGGTCG
    ACCGCAGGAC GTATGCTTGC TGAACTGCGG ACACTCAATA ATGACAACTT GCTGCTTAAA
    GCAGTGTGTG CAGTGAATAA TGTGGTGTAC GAGCTGTTTA ACAGGAAGCT GTATGAAGGA
    GCCTTTGGAT TGTCGATGAT TGTGTGTCAG GAGCTCTGTA AGGACTGCCC GCCCTCTCTT
    CCTATGGATC GGGTCAACCG CTGTTTCATG CTGACAGTGC AGTGTAGTCG CCGCGCAGGC
    CATCTGGAGC AGGCTCTGGA CTGGATTGTT CGCTGGATTC AGGCTTTGGG CTCACAGATT
    TTGGATCATC TCACTGAACC TGTGTCGATG TGGGTCAAAA CTAAGTGTGA TGCCGCCAGA
    GCAGGCGAGG ATGACACACG CCTGAGGACT CTGAGGGACG GGTTAGGAGA ATCCTTAGTG
    GATGAGGAGG TTATGATGTG TTTGTTAGAG GAGGAGTTGC GTGTATATAA GGAGCAGACT
    GGAGACACAG CACAGGAACG CTACAACACA CTCTGCGACC TCTTGGAAAT CTGCCATGAA
    GAAACCCCAC ACACACTAAG AAGAGCTACG TATCTGTGTG AGATGGCACA GGTTGTGTGC
    TATCAGGACT TCAGCCAGCA GACTGACTGC TCAGCAGTTG ATTTTACCCA CGAGGCTTTG
    CGGTTGCTGG AAGCAGAACC AGAGACGGCA GAGAATACTG ATAGGCTGAA GGATGAGAAA
    GCCCACGCTT CCCTGTGGCT CTATATCTGC ACTCTTGAGT CTAACCTGCA GGAGGCTGTG
    GACACAGAGA AGCGTTTGTG TGCAGTGCAG GAGGAGAATA AAAGAGATGT GAATATGGAT
    CCTGTTCCCA CAAATGATCT GGAGTACGAG GACAAACAGA AATCACAAGA GAGCCAGTTA
    GTCTACGATG GACTACGCTT CAACCTGCTG GCTCACAGCA AGCAAAGTGA ACCTCTAGAT
    AAATGTTTGT CTCTATGGCG GAGTCTCCTC AAGGGGGCTG TGCCTGCAGT TAGAGACCCT
    AAACTCACAG CTTCTTCCAT GACTTTGATG GCTGCCCTAT ACACACTTAT GGGCAAGCAC
    CTGCAAGCTC TGGAGGGATA TCAGCTTGCT GCCACCCTTT TCCATGTACT TGGTGATGGC
    CAGAGTAGTG CCAATGCCTT CTGCCACTCT ACCAGGATCT TGCTTTACCT GGGTTCACCG
    CAGCTTGCAC AGGTGGACTT AGAAAAAGCA GAAAAGAGCT TGTCATCTGT CACCATCTCT
    GAGGGCACAT CTATTATCTC CATGCTGGCC ATGTTGCAAA AAGCACAGCT ACACTATGCT
    CTAGGACAGG TGGAGAGTGG CGTGTCCTGC CTGATGGAGG TGATAAAGGA ATCTGCTCAG
    CATCATTCTA AGAGCTGGTA CCTGCTCAGG GCTCGAGCAC TGCAGACAGC CAGTGAATAC
    TTGAGTCTGG ACACACTGGC TTTGAACTCA CAGCTACGCC AGAGCATCAT GCAGCAGGGT
    CTAAAGACTC CAGACACTGC TCAATATGAA GGGTTGAAGC TGTTGTGTAG CCTGGTGATG
    ATGTTATTGG GCAACGGGTT TTACGGAGCG CCTGGACCAA ACACTGACAC TCGCTTTGTG
    GATAAAGGAG ACAGTGTTGT GTTTAAGTGG CTGCTGCTGA GTGAAGTGCT GGTGTGCTCG
    GAGAGAATGG TGATCACCAG GAGCAGCAGC GGAGCCGTTC ATGAAGCTAA AGCTCAGTGT
    CTAGAGGCTC TCAAACTGGC CACCAAACTA CAGACACTCA GCCACTGTGC TGAGCTGTTG
    GTGCTGAAGG CAGAGCTGGA GCTGATGAAG GGTGCAACTG AAGCTAGTAG TTTAGACCTA
    GAGCAAGTTA GACATTTACT TGATCTCTGC ACAGATTTTG GCCAGGGACG ACAACAGAAA
    TCTGAAGTGA AAATCAAACC ACGCAAAGGT CGTCCCGCTG CCCCTTCTTC CTCTGGTCAC
    ATCTCTGAGG AAGAGGATGA TCTGAGTGGG ATCCTCAGCT CTCGTGCCCT TCCGAAGGAG
    CCTGTGGAGA ACATGGCTAG ATTAGGAAGC CAAGGAGCTT CTCCCCCTCT AAAGCCCAAA
    TGCCAGCATG GCCTCTCCTG TCTGACCCAT GAAGAGACCT GCTCTTGTCT CTGCTGCACT
    GAGCTCAGCG TGGCTCGAGT CAGCATCCAC TGGGCACTAT TACAGGCAGA TCTCCAGACA
    GATCCCGAAC GCTCTCTCAG ACTACGCCAG TCTGCCAGGA AACGCTGCCG CTATGTTGTA
    ACTAAACTTC AGAGAAGTTT GGCAGCACTT GTACACTCCA AAAAATCACC TAATGTCTGC
    TTGTCACTGC TTCAGGCTGA ACAGGGTAAA GTGCATCTGG GTGCTGTGCT TCAACTGCTC
    AGAACTGGAG ACAAAGGGAA AGCTGCCATC TTGTGGGAGG AGATAGAAGC AGGATTAGAG
    GCAGTGACAC CTAAAGGAGC ACTGACACCA GAACTTGGGC CTATCAGAGC TACTCTACTG
    GGGGCTAAAG CCGTAGCCTG CTGCTTGGCG TTGGCAATGA AGAAACAGTG TATACCCGAA
    GAGCTGTTTT CTTCATTGTG GCACTGGAAT CCACCAAAGG TTAAACCGCA ATTAAAATCT
    AAGCATCGAT CCAAGAGTCC GGACGCAGCT CCTCTAGAGG CAGGTGTTAA ACATAACCCT
    AAAGTTAAGA GTGGACTTGA GGAGAATGAA AGCAGAAAAA CCAAAGAGAC CACTGTCATT
    TCGAAGAAAG CTAAAGACTC TGTGCCCAAA ATTACCATCA CAAAATCCTC CATGGTTTTT
    AAAACGCCCA AAGCAACGAG AACAACCCGG CCCAAATCTG TCTCCACTAG CACTGGCATC
    AGTGACCTGA GAGCATTTGA CTTCAATAAT GAGGTGCCTG ACATTTCAGT CAACTTCACA
    CCATCATTAC ACCGATCTAC AAACCAAAGT GCGACTGCAA AGTCAAAAGC GTCCTCAAAG
    GGTTCGTTCC AGGTTTACAA AGATTCTTCT CCTGCTGCAG AGAAAGCTGT AGTTGTCCCG
    GCTGCTCCTA AACGAACAAA GCGTTCTCGC TTCAAGGTTG AGTTCAGCGA TGAAAGCGAC
    AACGATGCTG CTCCTCTTGC TGTAATGGAA AAGTCAGAGA AAAAGAAAAA CACCTTTAAA
    ACATCACGCA CCTTGAAACC AACCCTTAAC ACCATCCCTA GTGACACCAA ACTTGTGAGC
    TCCACAGTAG ATCCAGAACC CTCCAGACGA ACACGGACCA CTAAGAAAAG CACAGCTCTT
    CCCTCCATTG GTGGCCTCTC TTCTGAGGAA GAACTTGGAG TATCTTCACA GACACGTACG
    CGTAGAGGAC GACCAAAGAA GAGCACCAGC TCAGCAGACA CAGAGGAGCC AGAGAGGATG
    AGGATGATTA AGGAGGAAGA ACAGAGGGTT CTTTTGAATG TCAGCATGGA GGAGCTCAGA
    GGCTCTGACA CTGAGACAAA AGACACTGGA AGTCCAGATG CTGACTGTGA GGTCTTGAGG
    AGAGATCTAG GTGCAGATTT TGGGCGAGAG TGTTTCAGTG AGCTCAGGAG AGCTGGACAG
    AATGGATCAG GATCTCACAC AGCTCTGCCA CATGCAACTA CATCTCCTGC AGACTTGTCA
    GTAGAGACGG TCCAGTCATA CCTGCGCTCC TCATGGCTCC TCCTGCACCA CTTCCCGTTT
    CCTTCTCTTT ATCCTCACAT CTGCTCATTA CTCGCTCAGT CATTCGGTCA AACGGACCCC
    ATCATCACAG CAATGCTGCA TGCACAGGCA CTGGGAGTGT CCACTCGCCA CCATATGACC
    CGTCACGTGG TTAACCAGTT CAGAAAGTTG AAGAAATCCT GTAACGATGT AGCAGAAGGT
    CTTGGAGCTC TGAGTCTGGA GGAAACATCT GGAGCAACGC AGTCAAAGAA ACTCTCTGCG
    TTGGAGCAGA TCTTTTCATT CACAGCATCA AACCCAATGC AGTTTCCACA GACACACTGT
    CAGCAGTTCA CACAGCAGCT GGAAGATCTA CCTGCAGGAG TGACCGTATG CTTGCTGTCA
    CTGACGGGGG TGCATCCTGA TGAAATTGGC AACACTATTC TTCTGACCCG ACTGGAGCGC
    GGCTCTACCC CAATCACAGT TCGAATCCCC ACTGCAGACA GAGAGCAATC TGTTGCTGGC
    CTGTTGGAAG AAATGGATGG AGTCTTGAAA GGACAAAAGG AAGTGAGCAC AGTGGCTGAG
    AAATCACAGT GGTGGGACGG AAGGAAGGCT TTAGATGCCC GTGTGGAGAA AATGCTGGAG
    GAGATGGAAG AGGCTTTAGG AGTGTGGCGA ACTCTTTTTC TGCCTTTGAC CTCTGATCCT
    AAGCTTGATG TTCAGGTAAA GAGCCTCCAA AAGGCTCTTA AATCAACAAA GATCACACGG
    GACACTCTCA AGGTGATTCT ATCAGCGTCT CCACTGCTGT CCCTGCCAGA CCTGCAGTGT
    CTTCTGGAAG GAATGAATGT GCAGGACAAG GACCTGCTGA AGCTCCTGCA GAGAGGCGTG
    GCTGAACTGA GAGGGAGGGA GGAGCCACAG GGACACACTG TTCTGATTTT AGACAAGTAC
    TTGCAGAGGT TGCCGTGGGA GAACATTGCT TGTCTGAAGT CTCAGTCGGT CACTCGAATG
    CCCTCATTAC ACGCGGTGCT TGGACACAGT CATCTGAGAG AGGTGGATAC AAGTTGTGTT
    CTATCTTGTG GTGTGAACCC TAAGCAGGTT TACTATGTGC TAAACCCAGA TGGAAACCTG
    CCCGACACTG AGAATCGCTT CAAAGAGTGG TTTACAGGTG AACGAACATG GCAGGGGGTT
    TGTGGAAGGG CTCCTGATCC GGAGAAACTA CAAGAGGCTG TGACCACTAA AGATCTCTAC
    ATATACATCG GACATGGTGC AGGAGCCCGC TTTCTGGATG CGCAGAGAGT TTTGAAGGTG
    CCTGTGAGGA CCGCAGCTCT TCTGTTCGGC TGCAGTAGTG CTGCTCTCAG TGTGCTTGGT
    CAGCAGGAGG GAACGGGCAT CATCCTCAGC TACCTGACCG CTGGATGTCC ATTGGTGCTG
    GGTAACCTGT GGGATGTGAC AGATCGTGAC TTGGATCGAT TCACATCGGC TCTGCTCCAG
    TCTTGGCTCT CTGCTGGTTC TGGCTCTTCA CTTCTTCAGC ATTTGGCGAA ATCACGCAGC
    GCAACTCATT TAAAACACAT AATCGGTGCT GCGCCCATTG CGTATGGTTT ACCGGTGCAT
    ATACACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa12112
    Clone ID Related Accession (Same CDS sequence) XM_021477220.1 , XM_001337833.4 , XM_001337833.5 , XM_017356662.1
    Accession Version XM_001337833.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6549bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product separin isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_001337833.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    ATGGCATTAA AGAGCTTGAA AACAGAAGAT TACGTTCAGC AGTTATCCTG TGTCAAGGAC 
    ACCATAGTCC TCCACAACGA ACTGAAGAAA CATGTGGATG GTGGCTTGGG TCCTTATGGT
    CGCACAATGT GTGATCGTAT CATCAGAGCC TGCAACCAGC GGCTGGGCAA CGGCGCTGTG
    GATGCTGGCC TTCAGGAGCG GATAGTGGAG CTGGTGGAAC TGGCTGTCCA AGGGTTTCAA
    CTGCTTGAAG AGTCCGGCAT GCAGAGCACC CCCTTTTATC TGGAGAAAAT AGTCTTTCAC
    ATCCTACAGA AAGTCGCAAG CCTTGGAGCT CATGGTCCTG CTTGTCGACT GGGTCAGCTC
    GTGTATAGAA GACTGAAATG TTTGTCTGCA GAGATTGATG ACTTTAATGT CCTGGTGCGG
    AATTGCTTTG CAGTCTTGTG GAACAGTCTC CTCTCTAACA AACCGAGTGC TTCCACCCTC
    CCTCCACGAG ACAGACTCCA CTGTCAGCTG CAAGCTTTAA CATTCAGACT CCTTGAACAG
    GGCTCCAGCT CGTCCTCTTC CCCATCCAAA GTTCCTCTGT TCGTGGAGGA AGCAGTAGTG
    GAGTACGAAC GATCATGTGG AGGTTTTACT CAAGACGATG TGAGTTTTCT GACCTCTGAA
    TTGCGTGAGC TGTTCTCTGG TGCTTTCATC AATCCTCAGG ACTCTGCATC AACTTCTTCA
    CTGCTGGCTG TGTGGTGTGA GGTGGCATTT AAAGTGTCTA AACTGCTCTG CAAAAGTAGC
    TTTTCACCTG AGGCTGTTGA ACTCTTGCGA GGCACTCTGA AGGAGGTTAA TGGCCACGTT
    GGCCTGCGTT CGGCTCTGGG TCTGGCAGAC CGGGCAGTGC AGATGCAGTG TGTGTTAAGT
    TCTGGAGGTG AATGCAGTAA GAGCTTCACT GAATGTGCCC GTGCTCTCCG GATGTTGCCT
    TGTGCCATGT CCAGCTCAGA GTCTCATGCC CTGTTAGAGG CTTGTCAGCT GGTGGTTTGG
    ACGTTGGAAG CCAGTCAGTG TAAAGGAATG GATGTGACCA CTTTGCTGGC TTGTTTTTCC
    TTTTTGGAAG AATATCAGGA ATTTCTGCTT GCACAGCAAA AGAACTCGTT TTCGCTGCAG
    GTCCAATACT CTCTTTGTTT CAGTTTTTAC CAGGGCTTCA TCTGCACATA TGATAGTCTT
    CATGCTTCTC AGGTGTCTGT GGGCGAGACC TTGGACAGAG TGCTATTGTT CTGCCGGTCG
    ACCGCAGGAC GTATGCTTGC TGAACTGCGG ACACTCAATA ATGACAACTT GCTGCTTAAA
    GCAGTGTGTG CAGTGAATAA TGTGGTGTAC GAGCTGTTTA ACAGGAAGCT GTATGAAGGA
    GCCTTTGGAT TGTCGATGAT TGTGTGTCAG GAGCTCTGTA AGGACTGCCC GCCCTCTCTT
    CCTATGGATC GGGTCAACCG CTGTTTCATG CTGACAGTGC AGTGTAGTCG CCGCGCAGGC
    CATCTGGAGC AGGCTCTGGA CTGGATTGTT CGCTGGATTC AGGCTTTGGG CTCACAGATT
    TTGGATCATC TCACTGAACC TGTGTCGATG TGGGTCAAAA CTAAGTGTGA TGCCGCCAGA
    GCAGGCGAGG ATGACACACG CCTGAGGACT CTGAGGGACG GGTTAGGAGA ATCCTTAGTG
    GATGAGGAGG TTATGATGTG TTTGTTAGAG GAGGAGTTGC GTGTATATAA GGAGCAGACT
    GGAGACACAG CACAGGAACG CTACAACACA CTCTGCGACC TCTTGGAAAT CTGCCATGAA
    GAAACCCCAC ACACACTAAG AAGAGCTACG TATCTGTGTG AGATGGCACA GGTTGTGTGC
    TATCAGGACT TCAGCCAGCA GACTGACTGC TCAGCAGTTG ATTTTACCCA CGAGGCTTTG
    CGGTTGCTGG AAGCAGAACC AGAGACGGCA GAGAATACTG ATAGGCTGAA GGATGAGAAA
    GCCCACGCTT CCCTGTGGCT CTATATCTGC ACTCTTGAGT CTAACCTGCA GGAGGCTGTG
    GACACAGAGA AGCGTTTGTG TGCAGTGCAG GAGGAGAATA AAAGAGATGT GAATATGGAT
    CCTGTTCCCA CAAATGATCT GGAGTACGAG GACAAACAGA AATCACAAGA GAGCCAGTTA
    GTCTACGATG GACTACGCTT CAACCTGCTG GCTCACAGCA AGCAAAGTGA ACCTCTAGAT
    AAATGTTTGT CTCTATGGCG GAGTCTCCTC AAGGGGGCTG TGCCTGCAGT TAGAGACCCT
    AAACTCACAG CTTCTTCCAT GACTTTGATG GCTGCCCTAT ACACACTTAT GGGCAAGCAC
    CTGCAAGCTC TGGAGGGATA TCAGCTTGCT GCCACCCTTT TCCATGTACT TGGTGATGGC
    CAGAGTAGTG CCAATGCCTT CTGCCACTCT ACCAGGATCT TGCTTTACCT GGGTTCACCG
    CAGCTTGCAC AGGTGGACTT AGAAAAAGCA GAAAAGAGCT TGTCATCTGT CACCATCTCT
    GAGGGCACAT CTATTATCTC CATGCTGGCC ATGTTGCAAA AAGCACAGCT ACACTATGCT
    CTAGGACAGG TGGAGAGTGG CGTGTCCTGC CTGATGGAGG TGATAAAGGA ATCTGCTCAG
    CATCATTCTA AGAGCTGGTA CCTGCTCAGG GCTCGAGCAC TGCAGACAGC CAGTGAATAC
    TTGAGTCTGG ACACACTGGC TTTGAACTCA CAGCTACGCC AGAGCATCAT GCAGCAGGGT
    CTAAAGACTC CAGACACTGC TCAATATGAA GGGTTGAAGC TGTTGTGTAG CCTGGTGATG
    ATGTTATTGG GCAACGGGTT TTACGGAGCG CCTGGACCAA ACACTGACAC TCGCTTTGTG
    GATAAAGGAG ACAGTGTTGT GTTTAAGTGG CTGCTGCTGA GTGAAGTGCT GGTGTGCTCG
    GAGAGAATGG TGATCACCAG GAGCAGCAGC GGAGCCGTTC ATGAAGCTAA AGCTCAGTGT
    CTAGAGGCTC TCAAACTGGC CACCAAACTA CAGACACTCA GCCACTGTGC TGAGCTGTTG
    GTGCTGAAGG CAGAGCTGGA GCTGATGAAG GGTGCAACTG AAGCTAGTAG TTTAGACCTA
    GAGCAAGTTA GACATTTACT TGATCTCTGC ACAGATTTTG GCCAGGGACG ACAACAGAAA
    TCTGAAGTGA AAATCAAACC ACGCAAAGGT CGTCCCGCTG CCCCTTCTTC CTCTGGTCAC
    ATCTCTGAGG AAGAGGATGA TCTGAGTGGG ATCCTCAGCT CTCGTGCCCT TCCGAAGGAG
    CCTGTGGAGA ACATGGCTAG ATTAGGAAGC CAAGGAGCTT CTCCCCCTCT AAAGCCCAAA
    TGCCAGCATG GCCTCTCCTG TCTGACCCAT GAAGAGACCT GCTCTTGTCT CTGCTGCACT
    GAGCTCAGCG TGGCTCGAGT CAGCATCCAC TGGGCACTAT TACAGGCAGA TCTCCAGACA
    GATCCCGAAC GCTCTCTCAG ACTACGCCAG TCTGCCAGGA AACGCTGCCG CTATGTTGTA
    ACTAAACTTC AGAGAAGTTT GGCAGCACTT GTACACTCCA AAAAATCACC TAATGTCTGC
    TTGTCACTGC TTCAGGCTGA ACAGGGTAAA GTGCATCTGG GTGCTGTGCT TCAACTGCTC
    AGAACTGGAG ACAAAGGGAA AGCTGCCATC TTGTGGGAGG AGATAGAAGC AGGATTAGAG
    GCAGTGACAC CTAAAGGAGC ACTGACACCA GAACTTGGGC CTATCAGAGC TACTCTACTG
    GGGGCTAAAG CCGTAGCCTG CTGCTTGGCG TTGGCAATGA AGAAACAGTG TATACCCGAA
    GAGCTGTTTT CTTCATTGTG GCACTGGAAT CCACCAAAGG TTAAACCGCA ATTAAAATCT
    AAGCATCGAT CCAAGAGTCC GGACGCAGCT CCTCTAGAGG CAGGTGTTAA ACATAACCCT
    AAAGTTAAGA GTGGACTTGA GGAGAATGAA AGCAGAAAAA CCAAAGAGAC CACTGTCATT
    TCGAAGAAAG CTAAAGACTC TGTGCCCAAA ATTACCATCA CAAAATCCTC CATGGTTTTT
    AAAACGCCCA AAGCAACGAG AACAACCCGG CCCAAATCTG TCTCCACTAG CACTGGCATC
    AGTGACCTGA GAGCATTTGA CTTCAATAAT GAGGTGCCTG ACATTTCAGT CAACTTCACA
    CCATCATTAC ACCGATCTAC AAACCAAAGT GCGACTGCAA AGTCAAAAGC GTCCTCAAAG
    GGTTCGTTCC AGGTTTACAA AGATTCTTCT CCTGCTGCAG AGAAAGCTGT AGTTGTCCCG
    GCTGCTCCTA AACGAACAAA GCGTTCTCGC TTCAAGGTTG AGTTCAGCGA TGAAAGCGAC
    AACGATGCTG CTCCTCTTGC TGTAATGGAA AAGTCAGAGA AAAAGAAAAA CACCTTTAAA
    ACATCACGCA CCTTGAAACC AACCCTTAAC ACCATCCCTA GTGACACCAA ACTTGTGAGC
    TCCACAGTAG ATCCAGAACC CTCCAGACGA ACACGGACCA CTAAGAAAAG CACAGCTCTT
    CCCTCCATTG GTGGCCTCTC TTCTGAGGAA GAACTTGGAG TATCTTCACA GACACGTACG
    CGTAGAGGAC GACCAAAGAA GAGCACCAGC TCAGCAGACA CAGAGGAGCC AGAGAGGATG
    AGGATGATTA AGGAGGAAGA ACAGAGGGTT CTTTTGAATG TCAGCATGGA GGAGCTCAGA
    GGCTCTGACA CTGAGACAAA AGACACTGGA AGTCCAGATG CTGACTGTGA GGTCTTGAGG
    AGAGATCTAG GTGCAGATTT TGGGCGAGAG TGTTTCAGTG AGCTCAGGAG AGCTGGACAG
    AATGGATCAG GATCTCACAC AGCTCTGCCA CATGCAACTA CATCTCCTGC AGACTTGTCA
    GTAGAGACGG TCCAGTCATA CCTGCGCTCC TCATGGCTCC TCCTGCACCA CTTCCCGTTT
    CCTTCTCTTT ATCCTCACAT CTGCTCATTA CTCGCTCAGT CATTCGGTCA AACGGACCCC
    ATCATCACAG CAATGCTGCA TGCACAGGCA CTGGGAGTGT CCACTCGCCA CCATATGACC
    CGTCACGTGG TTAACCAGTT CAGAAAGTTG AAGAAATCCT GTAACGATGT AGCAGAAGGT
    CTTGGAGCTC TGAGTCTGGA GGAAACATCT GGAGCAACGC AGTCAAAGAA ACTCTCTGCG
    TTGGAGCAGA TCTTTTCATT CACAGCATCA AACCCAATGC AGTTTCCACA GACACACTGT
    CAGCAGTTCA CACAGCAGCT GGAAGATCTA CCTGCAGGAG TGACCGTATG CTTGCTGTCA
    CTGACGGGGG TGCATCCTGA TGAAATTGGC AACACTATTC TTCTGACCCG ACTGGAGCGC
    GGCTCTACCC CAATCACAGT TCGAATCCCC ACTGCAGACA GAGAGCAATC TGTTGCTGGC
    CTGTTGGAAG AAATGGATGG AGTCTTGAAA GGACAAAAGG AAGTGAGCAC AGTGGCTGAG
    AAATCACAGT GGTGGGACGG AAGGAAGGCT TTAGATGCCC GTGTGGAGAA AATGCTGGAG
    GAGATGGAAG AGGCTTTAGG AGTGTGGCGA ACTCTTTTTC TGCCTTTGAC CTCTGATCCT
    AAGCTTGATG TTCAGGTAAA GAGCCTCCAA AAGGCTCTTA AATCAACAAA GATCACACGG
    GACACTCTCA AGGTGATTCT ATCAGCGTCT CCACTGCTGT CCCTGCCAGA CCTGCAGTGT
    CTTCTGGAAG GAATGAATGT GCAGGACAAG GACCTGCTGA AGCTCCTGCA GAGAGGCGTG
    GCTGAACTGA GAGGGAGGGA GGAGCCACAG GGACACACTG TTCTGATTTT AGACAAGTAC
    TTGCAGAGGT TGCCGTGGGA GAACATTGCT TGTCTGAAGT CTCAGTCGGT CACTCGAATG
    CCCTCATTAC ACGCGGTGCT TGGACACAGT CATCTGAGAG AGGTGGATAC AAGTTGTGTT
    CTATCTTGTG GTGTGAACCC TAAGCAGGTT TACTATGTGC TAAACCCAGA TGGAAACCTG
    CCCGACACTG AGAATCGCTT CAAAGAGTGG TTTACAGGTG AACGAACATG GCAGGGGGTT
    TGTGGAAGGG CTCCTGATCC GGAGAAACTA CAAGAGGCTG TGACCACTAA AGATCTCTAC
    ATATACATCG GACATGGTGC AGGAGCCCGC TTTCTGGATG CGCAGAGAGT TTTGAAGGTG
    CCTGTGAGGA CCGCAGCTCT TCTGTTCGGC TGCAGTAGTG CTGCTCTCAG TGTGCTTGGT
    CAGCAGGAGG GAACGGGCAT CATCCTCAGC TACCTGACCG CTGGATGTCC ATTGGTGCTG
    GGTAACCTGT GGGATGTGAC AGATCGTGAC TTGGATCGAT TCACATCGGC TCTGCTCCAG
    TCTTGGCTCT CTGCTGGTTC TGGCTCTTCA CTTCTTCAGC ATTTGGCGAA ATCACGCAGC
    GCAACTCATT TAAAACACAT AATCGGTGCT GCGCCCATTG CGTATGGTTT ACCGGTGCAT
    ATACACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_001337869.1
    CDS187..6735
    Translation

    Target ORF information:

    RefSeq Version XM_001337833.4
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio extra spindle pole bodies like 1, separase (espl1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_001337833.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    ATGGCATTAA AGAGCTTGAA AACAGAAGAT TACGTTCAGC AGTTATCCTG TGTCAAGGAC 
    ACCATAGTCC TCCACAACGA ACTGAAGAAA CATGTGGATG GTGGCTTGGG TCCTTATGGT
    CGCACAATGT GTGATCGTAT CATCAGAGCC TGCAACCAGC GGCTGGGCAA CGGCGCTGTG
    GATGCTGGCC TTCAGGAGCG GATAGTGGAG CTGGTGGAAC TGGCTGTCCA AGGGTTTCAA
    CTGCTTGAAG AGTCCGGCAT GCAGAGCACC CCCTTTTATC TGGAGAAAAT AGTCTTTCAC
    ATCCTACAGA AAGTCGCAAG CCTTGGAGCT CATGGTCCTG CTTGTCGACT GGGTCAGCTC
    GTGTATAGAA GACTGAAATG TTTGTCTGCA GAGATTGATG ACTTTAATGT CCTGGTGCGG
    AATTGCTTTG CAGTCTTGTG GAACAGTCTC CTCTCTAACA AACCGAGTGC TTCCACCCTC
    CCTCCACGAG ACAGACTCCA CTGTCAGCTG CAAGCTTTAA CATTCAGACT CCTTGAACAG
    GGCTCCAGCT CGTCCTCTTC CCCATCCAAA GTTCCTCTGT TCGTGGAGGA AGCAGTAGTG
    GAGTACGAAC GATCATGTGG AGGTTTTACT CAAGACGATG TGAGTTTTCT GACCTCTGAA
    TTGCGTGAGC TGTTCTCTGG TGCTTTCATC AATCCTCAGG ACTCTGCATC AACTTCTTCA
    CTGCTGGCTG TGTGGTGTGA GGTGGCATTT AAAGTGTCTA AACTGCTCTG CAAAAGTAGC
    TTTTCACCTG AGGCTGTTGA ACTCTTGCGA GGCACTCTGA AGGAGGTTAA TGGCCACGTT
    GGCCTGCGTT CGGCTCTGGG TCTGGCAGAC CGGGCAGTGC AGATGCAGTG TGTGTTAAGT
    TCTGGAGGTG AATGCAGTAA GAGCTTCACT GAATGTGCCC GTGCTCTCCG GATGTTGCCT
    TGTGCCATGT CCAGCTCAGA GTCTCATGCC CTGTTAGAGG CTTGTCAGCT GGTGGTTTGG
    ACGTTGGAAG CCAGTCAGTG TAAAGGAATG GATGTGACCA CTTTGCTGGC TTGTTTTTCC
    TTTTTGGAAG AATATCAGGA ATTTCTGCTT GCACAGCAAA AGAACTCGTT TTCGCTGCAG
    GTCCAATACT CTCTTTGTTT CAGTTTTTAC CAGGGCTTCA TCTGCACATA TGATAGTCTT
    CATGCTTCTC AGGTGTCTGT GGGCGAGACC TTGGACAGAG TGCTATTGTT CTGCCGGTCG
    ACCGCAGGAC GTATGCTTGC TGAACTGCGG ACACTCAATA ATGACAACTT GCTGCTTAAA
    GCAGTGTGTG CAGTGAATAA TGTGGTGTAC GAGCTGTTTA ACAGGAAGCT GTATGAAGGA
    GCCTTTGGAT TGTCGATGAT TGTGTGTCAG GAGCTCTGTA AGGACTGCCC GCCCTCTCTT
    CCTATGGATC GGGTCAACCG CTGTTTCATG CTGACAGTGC AGTGTAGTCG CCGCGCAGGC
    CATCTGGAGC AGGCTCTGGA CTGGATTGTT CGCTGGATTC AGGCTTTGGG CTCACAGATT
    TTGGATCATC TCACTGAACC TGTGTCGATG TGGGTCAAAA CTAAGTGTGA TGCCGCCAGA
    GCAGGCGAGG ATGACACACG CCTGAGGACT CTGAGGGACG GGTTAGGAGA ATCCTTAGTG
    GATGAGGAGG TTATGATGTG TTTGTTAGAG GAGGAGTTGC GTGTATATAA GGAGCAGACT
    GGAGACACAG CACAGGAACG CTACAACACA CTCTGCGACC TCTTGGAAAT CTGCCATGAA
    GAAACCCCAC ACACACTAAG AAGAGCTACG TATCTGTGTG AGATGGCACA GGTTGTGTGC
    TATCAGGACT TCAGCCAGCA GACTGACTGC TCAGCAGTTG ATTTTACCCA CGAGGCTTTG
    CGGTTGCTGG AAGCAGAACC AGAGACGGCA GAGAATACTG ATAGGCTGAA GGATGAGAAA
    GCCCACGCTT CCCTGTGGCT CTATATCTGC ACTCTTGAGT CTAACCTGCA GGAGGCTGTG
    GACACAGAGA AGCGTTTGTG TGCAGTGCAG GAGGAGAATA AAAGAGATGT GAATATGGAT
    CCTGTTCCCA CAAATGATCT GGAGTACGAG GACAAACAGA AATCACAAGA GAGCCAGTTA
    GTCTACGATG GACTACGCTT CAACCTGCTG GCTCACAGCA AGCAAAGTGA ACCTCTAGAT
    AAATGTTTGT CTCTATGGCG GAGTCTCCTC AAGGGGGCTG TGCCTGCAGT TAGAGACCCT
    AAACTCACAG CTTCTTCCAT GACTTTGATG GCTGCCCTAT ACACACTTAT GGGCAAGCAC
    CTGCAAGCTC TGGAGGGATA TCAGCTTGCT GCCACCCTTT TCCATGTACT TGGTGATGGC
    CAGAGTAGTG CCAATGCCTT CTGCCACTCT ACCAGGATCT TGCTTTACCT GGGTTCACCG
    CAGCTTGCAC AGGTGGACTT AGAAAAAGCA GAAAAGAGCT TGTCATCTGT CACCATCTCT
    GAGGGCACAT CTATTATCTC CATGCTGGCC ATGTTGCAAA AAGCACAGCT ACACTATGCT
    CTAGGACAGG TGGAGAGTGG CGTGTCCTGC CTGATGGAGG TGATAAAGGA ATCTGCTCAG
    CATCATTCTA AGAGCTGGTA CCTGCTCAGG GCTCGAGCAC TGCAGACAGC CAGTGAATAC
    TTGAGTCTGG ACACACTGGC TTTGAACTCA CAGCTACGCC AGAGCATCAT GCAGCAGGGT
    CTAAAGACTC CAGACACTGC TCAATATGAA GGGTTGAAGC TGTTGTGTAG CCTGGTGATG
    ATGTTATTGG GCAACGGGTT TTACGGAGCG CCTGGACCAA ACACTGACAC TCGCTTTGTG
    GATAAAGGAG ACAGTGTTGT GTTTAAGTGG CTGCTGCTGA GTGAAGTGCT GGTGTGCTCG
    GAGAGAATGG TGATCACCAG GAGCAGCAGC GGAGCCGTTC ATGAAGCTAA AGCTCAGTGT
    CTAGAGGCTC TCAAACTGGC CACCAAACTA CAGACACTCA GCCACTGTGC TGAGCTGTTG
    GTGCTGAAGG CAGAGCTGGA GCTGATGAAG GGTGCAACTG AAGCTAGTAG TTTAGACCTA
    GAGCAAGTTA GACATTTACT TGATCTCTGC ACAGATTTTG GCCAGGGACG ACAACAGAAA
    TCTGAAGTGA AAATCAAACC ACGCAAAGGT CGTCCCGCTG CCCCTTCTTC CTCTGGTCAC
    ATCTCTGAGG AAGAGGATGA TCTGAGTGGG ATCCTCAGCT CTCGTGCCCT TCCGAAGGAG
    CCTGTGGAGA ACATGGCTAG ATTAGGAAGC CAAGGAGCTT CTCCCCCTCT AAAGCCCAAA
    TGCCAGCATG GCCTCTCCTG TCTGACCCAT GAAGAGACCT GCTCTTGTCT CTGCTGCACT
    GAGCTCAGCG TGGCTCGAGT CAGCATCCAC TGGGCACTAT TACAGGCAGA TCTCCAGACA
    GATCCCGAAC GCTCTCTCAG ACTACGCCAG TCTGCCAGGA AACGCTGCCG CTATGTTGTA
    ACTAAACTTC AGAGAAGTTT GGCAGCACTT GTACACTCCA AAAAATCACC TAATGTCTGC
    TTGTCACTGC TTCAGGCTGA ACAGGGTAAA GTGCATCTGG GTGCTGTGCT TCAACTGCTC
    AGAACTGGAG ACAAAGGGAA AGCTGCCATC TTGTGGGAGG AGATAGAAGC AGGATTAGAG
    GCAGTGACAC CTAAAGGAGC ACTGACACCA GAACTTGGGC CTATCAGAGC TACTCTACTG
    GGGGCTAAAG CCGTAGCCTG CTGCTTGGCG TTGGCAATGA AGAAACAGTG TATACCCGAA
    GAGCTGTTTT CTTCATTGTG GCACTGGAAT CCACCAAAGG TTAAACCGCA ATTAAAATCT
    AAGCATCGAT CCAAGAGTCC GGACGCAGCT CCTCTAGAGG CAGGTGTTAA ACATAACCCT
    AAAGTTAAGA GTGGACTTGA GGAGAATGAA AGCAGAAAAA CCAAAGAGAC CACTGTCATT
    TCGAAGAAAG CTAAAGACTC TGTGCCCAAA ATTACCATCA CAAAATCCTC CATGGTTTTT
    AAAACGCCCA AAGCAACGAG AACAACCCGG CCCAAATCTG TCTCCACTAG CACTGGCATC
    AGTGACCTGA GAGCATTTGA CTTCAATAAT GAGGTGCCTG ACATTTCAGT CAACTTCACA
    CCATCATTAC ACCGATCTAC AAACCAAAGT GCGACTGCAA AGTCAAAAGC GTCCTCAAAG
    GGTTCGTTCC AGGTTTACAA AGATTCTTCT CCTGCTGCAG AGAAAGCTGT AGTTGTCCCG
    GCTGCTCCTA AACGAACAAA GCGTTCTCGC TTCAAGGTTG AGTTCAGCGA TGAAAGCGAC
    AACGATGCTG CTCCTCTTGC TGTAATGGAA AAGTCAGAGA AAAAGAAAAA CACCTTTAAA
    ACATCACGCA CCTTGAAACC AACCCTTAAC ACCATCCCTA GTGACACCAA ACTTGTGAGC
    TCCACAGTAG ATCCAGAACC CTCCAGACGA ACACGGACCA CTAAGAAAAG CACAGCTCTT
    CCCTCCATTG GTGGCCTCTC TTCTGAGGAA GAACTTGGAG TATCTTCACA GACACGTACG
    CGTAGAGGAC GACCAAAGAA GAGCACCAGC TCAGCAGACA CAGAGGAGCC AGAGAGGATG
    AGGATGATTA AGGAGGAAGA ACAGAGGGTT CTTTTGAATG TCAGCATGGA GGAGCTCAGA
    GGCTCTGACA CTGAGACAAA AGACACTGGA AGTCCAGATG CTGACTGTGA GGTCTTGAGG
    AGAGATCTAG GTGCAGATTT TGGGCGAGAG TGTTTCAGTG AGCTCAGGAG AGCTGGACAG
    AATGGATCAG GATCTCACAC AGCTCTGCCA CATGCAACTA CATCTCCTGC AGACTTGTCA
    GTAGAGACGG TCCAGTCATA CCTGCGCTCC TCATGGCTCC TCCTGCACCA CTTCCCGTTT
    CCTTCTCTTT ATCCTCACAT CTGCTCATTA CTCGCTCAGT CATTCGGTCA AACGGACCCC
    ATCATCACAG CAATGCTGCA TGCACAGGCA CTGGGAGTGT CCACTCGCCA CCATATGACC
    CGTCACGTGG TTAACCAGTT CAGAAAGTTG AAGAAATCCT GTAACGATGT AGCAGAAGGT
    CTTGGAGCTC TGAGTCTGGA GGAAACATCT GGAGCAACGC AGTCAAAGAA ACTCTCTGCG
    TTGGAGCAGA TCTTTTCATT CACAGCATCA AACCCAATGC AGTTTCCACA GACACACTGT
    CAGCAGTTCA CACAGCAGCT GGAAGATCTA CCTGCAGGAG TGACCGTATG CTTGCTGTCA
    CTGACGGGGG TGCATCCTGA TGAAATTGGC AACACTATTC TTCTGACCCG ACTGGAGCGC
    GGCTCTACCC CAATCACAGT TCGAATCCCC ACTGCAGACA GAGAGCAATC TGTTGCTGGC
    CTGTTGGAAG AAATGGATGG AGTCTTGAAA GGACAAAAGG AAGTGAGCAC AGTGGCTGAG
    AAATCACAGT GGTGGGACGG AAGGAAGGCT TTAGATGCCC GTGTGGAGAA AATGCTGGAG
    GAGATGGAAG AGGCTTTAGG AGTGTGGCGA ACTCTTTTTC TGCCTTTGAC CTCTGATCCT
    AAGCTTGATG TTCAGGTAAA GAGCCTCCAA AAGGCTCTTA AATCAACAAA GATCACACGG
    GACACTCTCA AGGTGATTCT ATCAGCGTCT CCACTGCTGT CCCTGCCAGA CCTGCAGTGT
    CTTCTGGAAG GAATGAATGT GCAGGACAAG GACCTGCTGA AGCTCCTGCA GAGAGGCGTG
    GCTGAACTGA GAGGGAGGGA GGAGCCACAG GGACACACTG TTCTGATTTT AGACAAGTAC
    TTGCAGAGGT TGCCGTGGGA GAACATTGCT TGTCTGAAGT CTCAGTCGGT CACTCGAATG
    CCCTCATTAC ACGCGGTGCT TGGACACAGT CATCTGAGAG AGGTGGATAC AAGTTGTGTT
    CTATCTTGTG GTGTGAACCC TAAGCAGGTT TACTATGTGC TAAACCCAGA TGGAAACCTG
    CCCGACACTG AGAATCGCTT CAAAGAGTGG TTTACAGGTG AACGAACATG GCAGGGGGTT
    TGTGGAAGGG CTCCTGATCC GGAGAAACTA CAAGAGGCTG TGACCACTAA AGATCTCTAC
    ATATACATCG GACATGGTGC AGGAGCCCGC TTTCTGGATG CGCAGAGAGT TTTGAAGGTG
    CCTGTGAGGA CCGCAGCTCT TCTGTTCGGC TGCAGTAGTG CTGCTCTCAG TGTGCTTGGT
    CAGCAGGAGG GAACGGGCAT CATCCTCAGC TACCTGACCG CTGGATGTCC ATTGGTGCTG
    GGTAACCTGT GGGATGTGAC AGATCGTGAC TTGGATCGAT TCACATCGGC TCTGCTCCAG
    TCTTGGCTCT CTGCTGGTTC TGGCTCTTCA CTTCTTCAGC ATTTGGCGAA ATCACGCAGC
    GCAACTCATT TAAAACACAT AATCGGTGCT GCGCCCATTG CGTATGGTTT ACCGGTGCAT
    ATACACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa12112
    Clone ID Related Accession (Same CDS sequence) XM_021477220.1 , XM_001337833.4 , XM_001337833.5 , XM_017356662.1
    Accession Version XM_001337833.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6549bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product separin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395382.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_001337833.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    ATGGCATTAA AGAGCTTGAA AACAGAAGAT TACGTTCAGC AGTTATCCTG TGTCAAGGAC 
    ACCATAGTCC TCCACAACGA ACTGAAGAAA CATGTGGATG GTGGCTTGGG TCCTTATGGT
    CGCACAATGT GTGATCGTAT CATCAGAGCC TGCAACCAGC GGCTGGGCAA CGGCGCTGTG
    GATGCTGGCC TTCAGGAGCG GATAGTGGAG CTGGTGGAAC TGGCTGTCCA AGGGTTTCAA
    CTGCTTGAAG AGTCCGGCAT GCAGAGCACC CCCTTTTATC TGGAGAAAAT AGTCTTTCAC
    ATCCTACAGA AAGTCGCAAG CCTTGGAGCT CATGGTCCTG CTTGTCGACT GGGTCAGCTC
    GTGTATAGAA GACTGAAATG TTTGTCTGCA GAGATTGATG ACTTTAATGT CCTGGTGCGG
    AATTGCTTTG CAGTCTTGTG GAACAGTCTC CTCTCTAACA AACCGAGTGC TTCCACCCTC
    CCTCCACGAG ACAGACTCCA CTGTCAGCTG CAAGCTTTAA CATTCAGACT CCTTGAACAG
    GGCTCCAGCT CGTCCTCTTC CCCATCCAAA GTTCCTCTGT TCGTGGAGGA AGCAGTAGTG
    GAGTACGAAC GATCATGTGG AGGTTTTACT CAAGACGATG TGAGTTTTCT GACCTCTGAA
    TTGCGTGAGC TGTTCTCTGG TGCTTTCATC AATCCTCAGG ACTCTGCATC AACTTCTTCA
    CTGCTGGCTG TGTGGTGTGA GGTGGCATTT AAAGTGTCTA AACTGCTCTG CAAAAGTAGC
    TTTTCACCTG AGGCTGTTGA ACTCTTGCGA GGCACTCTGA AGGAGGTTAA TGGCCACGTT
    GGCCTGCGTT CGGCTCTGGG TCTGGCAGAC CGGGCAGTGC AGATGCAGTG TGTGTTAAGT
    TCTGGAGGTG AATGCAGTAA GAGCTTCACT GAATGTGCCC GTGCTCTCCG GATGTTGCCT
    TGTGCCATGT CCAGCTCAGA GTCTCATGCC CTGTTAGAGG CTTGTCAGCT GGTGGTTTGG
    ACGTTGGAAG CCAGTCAGTG TAAAGGAATG GATGTGACCA CTTTGCTGGC TTGTTTTTCC
    TTTTTGGAAG AATATCAGGA ATTTCTGCTT GCACAGCAAA AGAACTCGTT TTCGCTGCAG
    GTCCAATACT CTCTTTGTTT CAGTTTTTAC CAGGGCTTCA TCTGCACATA TGATAGTCTT
    CATGCTTCTC AGGTGTCTGT GGGCGAGACC TTGGACAGAG TGCTATTGTT CTGCCGGTCG
    ACCGCAGGAC GTATGCTTGC TGAACTGCGG ACACTCAATA ATGACAACTT GCTGCTTAAA
    GCAGTGTGTG CAGTGAATAA TGTGGTGTAC GAGCTGTTTA ACAGGAAGCT GTATGAAGGA
    GCCTTTGGAT TGTCGATGAT TGTGTGTCAG GAGCTCTGTA AGGACTGCCC GCCCTCTCTT
    CCTATGGATC GGGTCAACCG CTGTTTCATG CTGACAGTGC AGTGTAGTCG CCGCGCAGGC
    CATCTGGAGC AGGCTCTGGA CTGGATTGTT CGCTGGATTC AGGCTTTGGG CTCACAGATT
    TTGGATCATC TCACTGAACC TGTGTCGATG TGGGTCAAAA CTAAGTGTGA TGCCGCCAGA
    GCAGGCGAGG ATGACACACG CCTGAGGACT CTGAGGGACG GGTTAGGAGA ATCCTTAGTG
    GATGAGGAGG TTATGATGTG TTTGTTAGAG GAGGAGTTGC GTGTATATAA GGAGCAGACT
    GGAGACACAG CACAGGAACG CTACAACACA CTCTGCGACC TCTTGGAAAT CTGCCATGAA
    GAAACCCCAC ACACACTAAG AAGAGCTACG TATCTGTGTG AGATGGCACA GGTTGTGTGC
    TATCAGGACT TCAGCCAGCA GACTGACTGC TCAGCAGTTG ATTTTACCCA CGAGGCTTTG
    CGGTTGCTGG AAGCAGAACC AGAGACGGCA GAGAATACTG ATAGGCTGAA GGATGAGAAA
    GCCCACGCTT CCCTGTGGCT CTATATCTGC ACTCTTGAGT CTAACCTGCA GGAGGCTGTG
    GACACAGAGA AGCGTTTGTG TGCAGTGCAG GAGGAGAATA AAAGAGATGT GAATATGGAT
    CCTGTTCCCA CAAATGATCT GGAGTACGAG GACAAACAGA AATCACAAGA GAGCCAGTTA
    GTCTACGATG GACTACGCTT CAACCTGCTG GCTCACAGCA AGCAAAGTGA ACCTCTAGAT
    AAATGTTTGT CTCTATGGCG GAGTCTCCTC AAGGGGGCTG TGCCTGCAGT TAGAGACCCT
    AAACTCACAG CTTCTTCCAT GACTTTGATG GCTGCCCTAT ACACACTTAT GGGCAAGCAC
    CTGCAAGCTC TGGAGGGATA TCAGCTTGCT GCCACCCTTT TCCATGTACT TGGTGATGGC
    CAGAGTAGTG CCAATGCCTT CTGCCACTCT ACCAGGATCT TGCTTTACCT GGGTTCACCG
    CAGCTTGCAC AGGTGGACTT AGAAAAAGCA GAAAAGAGCT TGTCATCTGT CACCATCTCT
    GAGGGCACAT CTATTATCTC CATGCTGGCC ATGTTGCAAA AAGCACAGCT ACACTATGCT
    CTAGGACAGG TGGAGAGTGG CGTGTCCTGC CTGATGGAGG TGATAAAGGA ATCTGCTCAG
    CATCATTCTA AGAGCTGGTA CCTGCTCAGG GCTCGAGCAC TGCAGACAGC CAGTGAATAC
    TTGAGTCTGG ACACACTGGC TTTGAACTCA CAGCTACGCC AGAGCATCAT GCAGCAGGGT
    CTAAAGACTC CAGACACTGC TCAATATGAA GGGTTGAAGC TGTTGTGTAG CCTGGTGATG
    ATGTTATTGG GCAACGGGTT TTACGGAGCG CCTGGACCAA ACACTGACAC TCGCTTTGTG
    GATAAAGGAG ACAGTGTTGT GTTTAAGTGG CTGCTGCTGA GTGAAGTGCT GGTGTGCTCG
    GAGAGAATGG TGATCACCAG GAGCAGCAGC GGAGCCGTTC ATGAAGCTAA AGCTCAGTGT
    CTAGAGGCTC TCAAACTGGC CACCAAACTA CAGACACTCA GCCACTGTGC TGAGCTGTTG
    GTGCTGAAGG CAGAGCTGGA GCTGATGAAG GGTGCAACTG AAGCTAGTAG TTTAGACCTA
    GAGCAAGTTA GACATTTACT TGATCTCTGC ACAGATTTTG GCCAGGGACG ACAACAGAAA
    TCTGAAGTGA AAATCAAACC ACGCAAAGGT CGTCCCGCTG CCCCTTCTTC CTCTGGTCAC
    ATCTCTGAGG AAGAGGATGA TCTGAGTGGG ATCCTCAGCT CTCGTGCCCT TCCGAAGGAG
    CCTGTGGAGA ACATGGCTAG ATTAGGAAGC CAAGGAGCTT CTCCCCCTCT AAAGCCCAAA
    TGCCAGCATG GCCTCTCCTG TCTGACCCAT GAAGAGACCT GCTCTTGTCT CTGCTGCACT
    GAGCTCAGCG TGGCTCGAGT CAGCATCCAC TGGGCACTAT TACAGGCAGA TCTCCAGACA
    GATCCCGAAC GCTCTCTCAG ACTACGCCAG TCTGCCAGGA AACGCTGCCG CTATGTTGTA
    ACTAAACTTC AGAGAAGTTT GGCAGCACTT GTACACTCCA AAAAATCACC TAATGTCTGC
    TTGTCACTGC TTCAGGCTGA ACAGGGTAAA GTGCATCTGG GTGCTGTGCT TCAACTGCTC
    AGAACTGGAG ACAAAGGGAA AGCTGCCATC TTGTGGGAGG AGATAGAAGC AGGATTAGAG
    GCAGTGACAC CTAAAGGAGC ACTGACACCA GAACTTGGGC CTATCAGAGC TACTCTACTG
    GGGGCTAAAG CCGTAGCCTG CTGCTTGGCG TTGGCAATGA AGAAACAGTG TATACCCGAA
    GAGCTGTTTT CTTCATTGTG GCACTGGAAT CCACCAAAGG TTAAACCGCA ATTAAAATCT
    AAGCATCGAT CCAAGAGTCC GGACGCAGCT CCTCTAGAGG CAGGTGTTAA ACATAACCCT
    AAAGTTAAGA GTGGACTTGA GGAGAATGAA AGCAGAAAAA CCAAAGAGAC CACTGTCATT
    TCGAAGAAAG CTAAAGACTC TGTGCCCAAA ATTACCATCA CAAAATCCTC CATGGTTTTT
    AAAACGCCCA AAGCAACGAG AACAACCCGG CCCAAATCTG TCTCCACTAG CACTGGCATC
    AGTGACCTGA GAGCATTTGA CTTCAATAAT GAGGTGCCTG ACATTTCAGT CAACTTCACA
    CCATCATTAC ACCGATCTAC AAACCAAAGT GCGACTGCAA AGTCAAAAGC GTCCTCAAAG
    GGTTCGTTCC AGGTTTACAA AGATTCTTCT CCTGCTGCAG AGAAAGCTGT AGTTGTCCCG
    GCTGCTCCTA AACGAACAAA GCGTTCTCGC TTCAAGGTTG AGTTCAGCGA TGAAAGCGAC
    AACGATGCTG CTCCTCTTGC TGTAATGGAA AAGTCAGAGA AAAAGAAAAA CACCTTTAAA
    ACATCACGCA CCTTGAAACC AACCCTTAAC ACCATCCCTA GTGACACCAA ACTTGTGAGC
    TCCACAGTAG ATCCAGAACC CTCCAGACGA ACACGGACCA CTAAGAAAAG CACAGCTCTT
    CCCTCCATTG GTGGCCTCTC TTCTGAGGAA GAACTTGGAG TATCTTCACA GACACGTACG
    CGTAGAGGAC GACCAAAGAA GAGCACCAGC TCAGCAGACA CAGAGGAGCC AGAGAGGATG
    AGGATGATTA AGGAGGAAGA ACAGAGGGTT CTTTTGAATG TCAGCATGGA GGAGCTCAGA
    GGCTCTGACA CTGAGACAAA AGACACTGGA AGTCCAGATG CTGACTGTGA GGTCTTGAGG
    AGAGATCTAG GTGCAGATTT TGGGCGAGAG TGTTTCAGTG AGCTCAGGAG AGCTGGACAG
    AATGGATCAG GATCTCACAC AGCTCTGCCA CATGCAACTA CATCTCCTGC AGACTTGTCA
    GTAGAGACGG TCCAGTCATA CCTGCGCTCC TCATGGCTCC TCCTGCACCA CTTCCCGTTT
    CCTTCTCTTT ATCCTCACAT CTGCTCATTA CTCGCTCAGT CATTCGGTCA AACGGACCCC
    ATCATCACAG CAATGCTGCA TGCACAGGCA CTGGGAGTGT CCACTCGCCA CCATATGACC
    CGTCACGTGG TTAACCAGTT CAGAAAGTTG AAGAAATCCT GTAACGATGT AGCAGAAGGT
    CTTGGAGCTC TGAGTCTGGA GGAAACATCT GGAGCAACGC AGTCAAAGAA ACTCTCTGCG
    TTGGAGCAGA TCTTTTCATT CACAGCATCA AACCCAATGC AGTTTCCACA GACACACTGT
    CAGCAGTTCA CACAGCAGCT GGAAGATCTA CCTGCAGGAG TGACCGTATG CTTGCTGTCA
    CTGACGGGGG TGCATCCTGA TGAAATTGGC AACACTATTC TTCTGACCCG ACTGGAGCGC
    GGCTCTACCC CAATCACAGT TCGAATCCCC ACTGCAGACA GAGAGCAATC TGTTGCTGGC
    CTGTTGGAAG AAATGGATGG AGTCTTGAAA GGACAAAAGG AAGTGAGCAC AGTGGCTGAG
    AAATCACAGT GGTGGGACGG AAGGAAGGCT TTAGATGCCC GTGTGGAGAA AATGCTGGAG
    GAGATGGAAG AGGCTTTAGG AGTGTGGCGA ACTCTTTTTC TGCCTTTGAC CTCTGATCCT
    AAGCTTGATG TTCAGGTAAA GAGCCTCCAA AAGGCTCTTA AATCAACAAA GATCACACGG
    GACACTCTCA AGGTGATTCT ATCAGCGTCT CCACTGCTGT CCCTGCCAGA CCTGCAGTGT
    CTTCTGGAAG GAATGAATGT GCAGGACAAG GACCTGCTGA AGCTCCTGCA GAGAGGCGTG
    GCTGAACTGA GAGGGAGGGA GGAGCCACAG GGACACACTG TTCTGATTTT AGACAAGTAC
    TTGCAGAGGT TGCCGTGGGA GAACATTGCT TGTCTGAAGT CTCAGTCGGT CACTCGAATG
    CCCTCATTAC ACGCGGTGCT TGGACACAGT CATCTGAGAG AGGTGGATAC AAGTTGTGTT
    CTATCTTGTG GTGTGAACCC TAAGCAGGTT TACTATGTGC TAAACCCAGA TGGAAACCTG
    CCCGACACTG AGAATCGCTT CAAAGAGTGG TTTACAGGTG AACGAACATG GCAGGGGGTT
    TGTGGAAGGG CTCCTGATCC GGAGAAACTA CAAGAGGCTG TGACCACTAA AGATCTCTAC
    ATATACATCG GACATGGTGC AGGAGCCCGC TTTCTGGATG CGCAGAGAGT TTTGAAGGTG
    CCTGTGAGGA CCGCAGCTCT TCTGTTCGGC TGCAGTAGTG CTGCTCTCAG TGTGCTTGGT
    CAGCAGGAGG GAACGGGCAT CATCCTCAGC TACCTGACCG CTGGATGTCC ATTGGTGCTG
    GGTAACCTGT GGGATGTGAC AGATCGTGAC TTGGATCGAT TCACATCGGC TCTGCTCCAG
    TCTTGGCTCT CTGCTGGTTC TGGCTCTTCA CTTCTTCAGC ATTTGGCGAA ATCACGCAGC
    GCAACTCATT TAAAACACAT AATCGGTGCT GCGCCCATTG CGTATGGTTT ACCGGTGCAT
    ATACACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_001337869.1
    CDS210..6758
    Translation

    Target ORF information:

    RefSeq Version XM_001337833.5
    Organism Danio rerio(zebrafish)
    Definition Danio rerio extra spindle pole bodies like 1, separase (espl1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_001337833.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    ATGGCATTAA AGAGCTTGAA AACAGAAGAT TACGTTCAGC AGTTATCCTG TGTCAAGGAC 
    ACCATAGTCC TCCACAACGA ACTGAAGAAA CATGTGGATG GTGGCTTGGG TCCTTATGGT
    CGCACAATGT GTGATCGTAT CATCAGAGCC TGCAACCAGC GGCTGGGCAA CGGCGCTGTG
    GATGCTGGCC TTCAGGAGCG GATAGTGGAG CTGGTGGAAC TGGCTGTCCA AGGGTTTCAA
    CTGCTTGAAG AGTCCGGCAT GCAGAGCACC CCCTTTTATC TGGAGAAAAT AGTCTTTCAC
    ATCCTACAGA AAGTCGCAAG CCTTGGAGCT CATGGTCCTG CTTGTCGACT GGGTCAGCTC
    GTGTATAGAA GACTGAAATG TTTGTCTGCA GAGATTGATG ACTTTAATGT CCTGGTGCGG
    AATTGCTTTG CAGTCTTGTG GAACAGTCTC CTCTCTAACA AACCGAGTGC TTCCACCCTC
    CCTCCACGAG ACAGACTCCA CTGTCAGCTG CAAGCTTTAA CATTCAGACT CCTTGAACAG
    GGCTCCAGCT CGTCCTCTTC CCCATCCAAA GTTCCTCTGT TCGTGGAGGA AGCAGTAGTG
    GAGTACGAAC GATCATGTGG AGGTTTTACT CAAGACGATG TGAGTTTTCT GACCTCTGAA
    TTGCGTGAGC TGTTCTCTGG TGCTTTCATC AATCCTCAGG ACTCTGCATC AACTTCTTCA
    CTGCTGGCTG TGTGGTGTGA GGTGGCATTT AAAGTGTCTA AACTGCTCTG CAAAAGTAGC
    TTTTCACCTG AGGCTGTTGA ACTCTTGCGA GGCACTCTGA AGGAGGTTAA TGGCCACGTT
    GGCCTGCGTT CGGCTCTGGG TCTGGCAGAC CGGGCAGTGC AGATGCAGTG TGTGTTAAGT
    TCTGGAGGTG AATGCAGTAA GAGCTTCACT GAATGTGCCC GTGCTCTCCG GATGTTGCCT
    TGTGCCATGT CCAGCTCAGA GTCTCATGCC CTGTTAGAGG CTTGTCAGCT GGTGGTTTGG
    ACGTTGGAAG CCAGTCAGTG TAAAGGAATG GATGTGACCA CTTTGCTGGC TTGTTTTTCC
    TTTTTGGAAG AATATCAGGA ATTTCTGCTT GCACAGCAAA AGAACTCGTT TTCGCTGCAG
    GTCCAATACT CTCTTTGTTT CAGTTTTTAC CAGGGCTTCA TCTGCACATA TGATAGTCTT
    CATGCTTCTC AGGTGTCTGT GGGCGAGACC TTGGACAGAG TGCTATTGTT CTGCCGGTCG
    ACCGCAGGAC GTATGCTTGC TGAACTGCGG ACACTCAATA ATGACAACTT GCTGCTTAAA
    GCAGTGTGTG CAGTGAATAA TGTGGTGTAC GAGCTGTTTA ACAGGAAGCT GTATGAAGGA
    GCCTTTGGAT TGTCGATGAT TGTGTGTCAG GAGCTCTGTA AGGACTGCCC GCCCTCTCTT
    CCTATGGATC GGGTCAACCG CTGTTTCATG CTGACAGTGC AGTGTAGTCG CCGCGCAGGC
    CATCTGGAGC AGGCTCTGGA CTGGATTGTT CGCTGGATTC AGGCTTTGGG CTCACAGATT
    TTGGATCATC TCACTGAACC TGTGTCGATG TGGGTCAAAA CTAAGTGTGA TGCCGCCAGA
    GCAGGCGAGG ATGACACACG CCTGAGGACT CTGAGGGACG GGTTAGGAGA ATCCTTAGTG
    GATGAGGAGG TTATGATGTG TTTGTTAGAG GAGGAGTTGC GTGTATATAA GGAGCAGACT
    GGAGACACAG CACAGGAACG CTACAACACA CTCTGCGACC TCTTGGAAAT CTGCCATGAA
    GAAACCCCAC ACACACTAAG AAGAGCTACG TATCTGTGTG AGATGGCACA GGTTGTGTGC
    TATCAGGACT TCAGCCAGCA GACTGACTGC TCAGCAGTTG ATTTTACCCA CGAGGCTTTG
    CGGTTGCTGG AAGCAGAACC AGAGACGGCA GAGAATACTG ATAGGCTGAA GGATGAGAAA
    GCCCACGCTT CCCTGTGGCT CTATATCTGC ACTCTTGAGT CTAACCTGCA GGAGGCTGTG
    GACACAGAGA AGCGTTTGTG TGCAGTGCAG GAGGAGAATA AAAGAGATGT GAATATGGAT
    CCTGTTCCCA CAAATGATCT GGAGTACGAG GACAAACAGA AATCACAAGA GAGCCAGTTA
    GTCTACGATG GACTACGCTT CAACCTGCTG GCTCACAGCA AGCAAAGTGA ACCTCTAGAT
    AAATGTTTGT CTCTATGGCG GAGTCTCCTC AAGGGGGCTG TGCCTGCAGT TAGAGACCCT
    AAACTCACAG CTTCTTCCAT GACTTTGATG GCTGCCCTAT ACACACTTAT GGGCAAGCAC
    CTGCAAGCTC TGGAGGGATA TCAGCTTGCT GCCACCCTTT TCCATGTACT TGGTGATGGC
    CAGAGTAGTG CCAATGCCTT CTGCCACTCT ACCAGGATCT TGCTTTACCT GGGTTCACCG
    CAGCTTGCAC AGGTGGACTT AGAAAAAGCA GAAAAGAGCT TGTCATCTGT CACCATCTCT
    GAGGGCACAT CTATTATCTC CATGCTGGCC ATGTTGCAAA AAGCACAGCT ACACTATGCT
    CTAGGACAGG TGGAGAGTGG CGTGTCCTGC CTGATGGAGG TGATAAAGGA ATCTGCTCAG
    CATCATTCTA AGAGCTGGTA CCTGCTCAGG GCTCGAGCAC TGCAGACAGC CAGTGAATAC
    TTGAGTCTGG ACACACTGGC TTTGAACTCA CAGCTACGCC AGAGCATCAT GCAGCAGGGT
    CTAAAGACTC CAGACACTGC TCAATATGAA GGGTTGAAGC TGTTGTGTAG CCTGGTGATG
    ATGTTATTGG GCAACGGGTT TTACGGAGCG CCTGGACCAA ACACTGACAC TCGCTTTGTG
    GATAAAGGAG ACAGTGTTGT GTTTAAGTGG CTGCTGCTGA GTGAAGTGCT GGTGTGCTCG
    GAGAGAATGG TGATCACCAG GAGCAGCAGC GGAGCCGTTC ATGAAGCTAA AGCTCAGTGT
    CTAGAGGCTC TCAAACTGGC CACCAAACTA CAGACACTCA GCCACTGTGC TGAGCTGTTG
    GTGCTGAAGG CAGAGCTGGA GCTGATGAAG GGTGCAACTG AAGCTAGTAG TTTAGACCTA
    GAGCAAGTTA GACATTTACT TGATCTCTGC ACAGATTTTG GCCAGGGACG ACAACAGAAA
    TCTGAAGTGA AAATCAAACC ACGCAAAGGT CGTCCCGCTG CCCCTTCTTC CTCTGGTCAC
    ATCTCTGAGG AAGAGGATGA TCTGAGTGGG ATCCTCAGCT CTCGTGCCCT TCCGAAGGAG
    CCTGTGGAGA ACATGGCTAG ATTAGGAAGC CAAGGAGCTT CTCCCCCTCT AAAGCCCAAA
    TGCCAGCATG GCCTCTCCTG TCTGACCCAT GAAGAGACCT GCTCTTGTCT CTGCTGCACT
    GAGCTCAGCG TGGCTCGAGT CAGCATCCAC TGGGCACTAT TACAGGCAGA TCTCCAGACA
    GATCCCGAAC GCTCTCTCAG ACTACGCCAG TCTGCCAGGA AACGCTGCCG CTATGTTGTA
    ACTAAACTTC AGAGAAGTTT GGCAGCACTT GTACACTCCA AAAAATCACC TAATGTCTGC
    TTGTCACTGC TTCAGGCTGA ACAGGGTAAA GTGCATCTGG GTGCTGTGCT TCAACTGCTC
    AGAACTGGAG ACAAAGGGAA AGCTGCCATC TTGTGGGAGG AGATAGAAGC AGGATTAGAG
    GCAGTGACAC CTAAAGGAGC ACTGACACCA GAACTTGGGC CTATCAGAGC TACTCTACTG
    GGGGCTAAAG CCGTAGCCTG CTGCTTGGCG TTGGCAATGA AGAAACAGTG TATACCCGAA
    GAGCTGTTTT CTTCATTGTG GCACTGGAAT CCACCAAAGG TTAAACCGCA ATTAAAATCT
    AAGCATCGAT CCAAGAGTCC GGACGCAGCT CCTCTAGAGG CAGGTGTTAA ACATAACCCT
    AAAGTTAAGA GTGGACTTGA GGAGAATGAA AGCAGAAAAA CCAAAGAGAC CACTGTCATT
    TCGAAGAAAG CTAAAGACTC TGTGCCCAAA ATTACCATCA CAAAATCCTC CATGGTTTTT
    AAAACGCCCA AAGCAACGAG AACAACCCGG CCCAAATCTG TCTCCACTAG CACTGGCATC
    AGTGACCTGA GAGCATTTGA CTTCAATAAT GAGGTGCCTG ACATTTCAGT CAACTTCACA
    CCATCATTAC ACCGATCTAC AAACCAAAGT GCGACTGCAA AGTCAAAAGC GTCCTCAAAG
    GGTTCGTTCC AGGTTTACAA AGATTCTTCT CCTGCTGCAG AGAAAGCTGT AGTTGTCCCG
    GCTGCTCCTA AACGAACAAA GCGTTCTCGC TTCAAGGTTG AGTTCAGCGA TGAAAGCGAC
    AACGATGCTG CTCCTCTTGC TGTAATGGAA AAGTCAGAGA AAAAGAAAAA CACCTTTAAA
    ACATCACGCA CCTTGAAACC AACCCTTAAC ACCATCCCTA GTGACACCAA ACTTGTGAGC
    TCCACAGTAG ATCCAGAACC CTCCAGACGA ACACGGACCA CTAAGAAAAG CACAGCTCTT
    CCCTCCATTG GTGGCCTCTC TTCTGAGGAA GAACTTGGAG TATCTTCACA GACACGTACG
    CGTAGAGGAC GACCAAAGAA GAGCACCAGC TCAGCAGACA CAGAGGAGCC AGAGAGGATG
    AGGATGATTA AGGAGGAAGA ACAGAGGGTT CTTTTGAATG TCAGCATGGA GGAGCTCAGA
    GGCTCTGACA CTGAGACAAA AGACACTGGA AGTCCAGATG CTGACTGTGA GGTCTTGAGG
    AGAGATCTAG GTGCAGATTT TGGGCGAGAG TGTTTCAGTG AGCTCAGGAG AGCTGGACAG
    AATGGATCAG GATCTCACAC AGCTCTGCCA CATGCAACTA CATCTCCTGC AGACTTGTCA
    GTAGAGACGG TCCAGTCATA CCTGCGCTCC TCATGGCTCC TCCTGCACCA CTTCCCGTTT
    CCTTCTCTTT ATCCTCACAT CTGCTCATTA CTCGCTCAGT CATTCGGTCA AACGGACCCC
    ATCATCACAG CAATGCTGCA TGCACAGGCA CTGGGAGTGT CCACTCGCCA CCATATGACC
    CGTCACGTGG TTAACCAGTT CAGAAAGTTG AAGAAATCCT GTAACGATGT AGCAGAAGGT
    CTTGGAGCTC TGAGTCTGGA GGAAACATCT GGAGCAACGC AGTCAAAGAA ACTCTCTGCG
    TTGGAGCAGA TCTTTTCATT CACAGCATCA AACCCAATGC AGTTTCCACA GACACACTGT
    CAGCAGTTCA CACAGCAGCT GGAAGATCTA CCTGCAGGAG TGACCGTATG CTTGCTGTCA
    CTGACGGGGG TGCATCCTGA TGAAATTGGC AACACTATTC TTCTGACCCG ACTGGAGCGC
    GGCTCTACCC CAATCACAGT TCGAATCCCC ACTGCAGACA GAGAGCAATC TGTTGCTGGC
    CTGTTGGAAG AAATGGATGG AGTCTTGAAA GGACAAAAGG AAGTGAGCAC AGTGGCTGAG
    AAATCACAGT GGTGGGACGG AAGGAAGGCT TTAGATGCCC GTGTGGAGAA AATGCTGGAG
    GAGATGGAAG AGGCTTTAGG AGTGTGGCGA ACTCTTTTTC TGCCTTTGAC CTCTGATCCT
    AAGCTTGATG TTCAGGTAAA GAGCCTCCAA AAGGCTCTTA AATCAACAAA GATCACACGG
    GACACTCTCA AGGTGATTCT ATCAGCGTCT CCACTGCTGT CCCTGCCAGA CCTGCAGTGT
    CTTCTGGAAG GAATGAATGT GCAGGACAAG GACCTGCTGA AGCTCCTGCA GAGAGGCGTG
    GCTGAACTGA GAGGGAGGGA GGAGCCACAG GGACACACTG TTCTGATTTT AGACAAGTAC
    TTGCAGAGGT TGCCGTGGGA GAACATTGCT TGTCTGAAGT CTCAGTCGGT CACTCGAATG
    CCCTCATTAC ACGCGGTGCT TGGACACAGT CATCTGAGAG AGGTGGATAC AAGTTGTGTT
    CTATCTTGTG GTGTGAACCC TAAGCAGGTT TACTATGTGC TAAACCCAGA TGGAAACCTG
    CCCGACACTG AGAATCGCTT CAAAGAGTGG TTTACAGGTG AACGAACATG GCAGGGGGTT
    TGTGGAAGGG CTCCTGATCC GGAGAAACTA CAAGAGGCTG TGACCACTAA AGATCTCTAC
    ATATACATCG GACATGGTGC AGGAGCCCGC TTTCTGGATG CGCAGAGAGT TTTGAAGGTG
    CCTGTGAGGA CCGCAGCTCT TCTGTTCGGC TGCAGTAGTG CTGCTCTCAG TGTGCTTGGT
    CAGCAGGAGG GAACGGGCAT CATCCTCAGC TACCTGACCG CTGGATGTCC ATTGGTGCTG
    GGTAACCTGT GGGATGTGAC AGATCGTGAC TTGGATCGAT TCACATCGGC TCTGCTCCAG
    TCTTGGCTCT CTGCTGGTTC TGGCTCTTCA CTTCTTCAGC ATTTGGCGAA ATCACGCAGC
    GCAACTCATT TAAAACACAT AATCGGTGCT GCGCCCATTG CGTATGGTTT ACCGGTGCAT
    ATACACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa12112
    Clone ID Related Accession (Same CDS sequence) XM_021477220.1 , XM_001337833.4 , XM_001337833.5 , XM_017356662.1
    Accession Version XM_017356662.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6549bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product separin isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    ATGGCATTAA AGAGCTTGAA AACAGAAGAT TACGTTCAGC AGTTATCCTG TGTCAAGGAC 
    ACCATAGTCC TCCACAACGA ACTGAAGAAA CATGTGGATG GTGGCTTGGG TCCTTATGGT
    CGCACAATGT GTGATCGTAT CATCAGAGCC TGCAACCAGC GGCTGGGCAA CGGCGCTGTG
    GATGCTGGCC TTCAGGAGCG GATAGTGGAG CTGGTGGAAC TGGCTGTCCA AGGGTTTCAA
    CTGCTTGAAG AGTCCGGCAT GCAGAGCACC CCCTTTTATC TGGAGAAAAT AGTCTTTCAC
    ATCCTACAGA AAGTCGCAAG CCTTGGAGCT CATGGTCCTG CTTGTCGACT GGGTCAGCTC
    GTGTATAGAA GACTGAAATG TTTGTCTGCA GAGATTGATG ACTTTAATGT CCTGGTGCGG
    AATTGCTTTG CAGTCTTGTG GAACAGTCTC CTCTCTAACA AACCGAGTGC TTCCACCCTC
    CCTCCACGAG ACAGACTCCA CTGTCAGCTG CAAGCTTTAA CATTCAGACT CCTTGAACAG
    GGCTCCAGCT CGTCCTCTTC CCCATCCAAA GTTCCTCTGT TCGTGGAGGA AGCAGTAGTG
    GAGTACGAAC GATCATGTGG AGGTTTTACT CAAGACGATG TGAGTTTTCT GACCTCTGAA
    TTGCGTGAGC TGTTCTCTGG TGCTTTCATC AATCCTCAGG ACTCTGCATC AACTTCTTCA
    CTGCTGGCTG TGTGGTGTGA GGTGGCATTT AAAGTGTCTA AACTGCTCTG CAAAAGTAGC
    TTTTCACCTG AGGCTGTTGA ACTCTTGCGA GGCACTCTGA AGGAGGTTAA TGGCCACGTT
    GGCCTGCGTT CGGCTCTGGG TCTGGCAGAC CGGGCAGTGC AGATGCAGTG TGTGTTAAGT
    TCTGGAGGTG AATGCAGTAA GAGCTTCACT GAATGTGCCC GTGCTCTCCG GATGTTGCCT
    TGTGCCATGT CCAGCTCAGA GTCTCATGCC CTGTTAGAGG CTTGTCAGCT GGTGGTTTGG
    ACGTTGGAAG CCAGTCAGTG TAAAGGAATG GATGTGACCA CTTTGCTGGC TTGTTTTTCC
    TTTTTGGAAG AATATCAGGA ATTTCTGCTT GCACAGCAAA AGAACTCGTT TTCGCTGCAG
    GTCCAATACT CTCTTTGTTT CAGTTTTTAC CAGGGCTTCA TCTGCACATA TGATAGTCTT
    CATGCTTCTC AGGTGTCTGT GGGCGAGACC TTGGACAGAG TGCTATTGTT CTGCCGGTCG
    ACCGCAGGAC GTATGCTTGC TGAACTGCGG ACACTCAATA ATGACAACTT GCTGCTTAAA
    GCAGTGTGTG CAGTGAATAA TGTGGTGTAC GAGCTGTTTA ACAGGAAGCT GTATGAAGGA
    GCCTTTGGAT TGTCGATGAT TGTGTGTCAG GAGCTCTGTA AGGACTGCCC GCCCTCTCTT
    CCTATGGATC GGGTCAACCG CTGTTTCATG CTGACAGTGC AGTGTAGTCG CCGCGCAGGC
    CATCTGGAGC AGGCTCTGGA CTGGATTGTT CGCTGGATTC AGGCTTTGGG CTCACAGATT
    TTGGATCATC TCACTGAACC TGTGTCGATG TGGGTCAAAA CTAAGTGTGA TGCCGCCAGA
    GCAGGCGAGG ATGACACACG CCTGAGGACT CTGAGGGACG GGTTAGGAGA ATCCTTAGTG
    GATGAGGAGG TTATGATGTG TTTGTTAGAG GAGGAGTTGC GTGTATATAA GGAGCAGACT
    GGAGACACAG CACAGGAACG CTACAACACA CTCTGCGACC TCTTGGAAAT CTGCCATGAA
    GAAACCCCAC ACACACTAAG AAGAGCTACG TATCTGTGTG AGATGGCACA GGTTGTGTGC
    TATCAGGACT TCAGCCAGCA GACTGACTGC TCAGCAGTTG ATTTTACCCA CGAGGCTTTG
    CGGTTGCTGG AAGCAGAACC AGAGACGGCA GAGAATACTG ATAGGCTGAA GGATGAGAAA
    GCCCACGCTT CCCTGTGGCT CTATATCTGC ACTCTTGAGT CTAACCTGCA GGAGGCTGTG
    GACACAGAGA AGCGTTTGTG TGCAGTGCAG GAGGAGAATA AAAGAGATGT GAATATGGAT
    CCTGTTCCCA CAAATGATCT GGAGTACGAG GACAAACAGA AATCACAAGA GAGCCAGTTA
    GTCTACGATG GACTACGCTT CAACCTGCTG GCTCACAGCA AGCAAAGTGA ACCTCTAGAT
    AAATGTTTGT CTCTATGGCG GAGTCTCCTC AAGGGGGCTG TGCCTGCAGT TAGAGACCCT
    AAACTCACAG CTTCTTCCAT GACTTTGATG GCTGCCCTAT ACACACTTAT GGGCAAGCAC
    CTGCAAGCTC TGGAGGGATA TCAGCTTGCT GCCACCCTTT TCCATGTACT TGGTGATGGC
    CAGAGTAGTG CCAATGCCTT CTGCCACTCT ACCAGGATCT TGCTTTACCT GGGTTCACCG
    CAGCTTGCAC AGGTGGACTT AGAAAAAGCA GAAAAGAGCT TGTCATCTGT CACCATCTCT
    GAGGGCACAT CTATTATCTC CATGCTGGCC ATGTTGCAAA AAGCACAGCT ACACTATGCT
    CTAGGACAGG TGGAGAGTGG CGTGTCCTGC CTGATGGAGG TGATAAAGGA ATCTGCTCAG
    CATCATTCTA AGAGCTGGTA CCTGCTCAGG GCTCGAGCAC TGCAGACAGC CAGTGAATAC
    TTGAGTCTGG ACACACTGGC TTTGAACTCA CAGCTACGCC AGAGCATCAT GCAGCAGGGT
    CTAAAGACTC CAGACACTGC TCAATATGAA GGGTTGAAGC TGTTGTGTAG CCTGGTGATG
    ATGTTATTGG GCAACGGGTT TTACGGAGCG CCTGGACCAA ACACTGACAC TCGCTTTGTG
    GATAAAGGAG ACAGTGTTGT GTTTAAGTGG CTGCTGCTGA GTGAAGTGCT GGTGTGCTCG
    GAGAGAATGG TGATCACCAG GAGCAGCAGC GGAGCCGTTC ATGAAGCTAA AGCTCAGTGT
    CTAGAGGCTC TCAAACTGGC CACCAAACTA CAGACACTCA GCCACTGTGC TGAGCTGTTG
    GTGCTGAAGG CAGAGCTGGA GCTGATGAAG GGTGCAACTG AAGCTAGTAG TTTAGACCTA
    GAGCAAGTTA GACATTTACT TGATCTCTGC ACAGATTTTG GCCAGGGACG ACAACAGAAA
    TCTGAAGTGA AAATCAAACC ACGCAAAGGT CGTCCCGCTG CCCCTTCTTC CTCTGGTCAC
    ATCTCTGAGG AAGAGGATGA TCTGAGTGGG ATCCTCAGCT CTCGTGCCCT TCCGAAGGAG
    CCTGTGGAGA ACATGGCTAG ATTAGGAAGC CAAGGAGCTT CTCCCCCTCT AAAGCCCAAA
    TGCCAGCATG GCCTCTCCTG TCTGACCCAT GAAGAGACCT GCTCTTGTCT CTGCTGCACT
    GAGCTCAGCG TGGCTCGAGT CAGCATCCAC TGGGCACTAT TACAGGCAGA TCTCCAGACA
    GATCCCGAAC GCTCTCTCAG ACTACGCCAG TCTGCCAGGA AACGCTGCCG CTATGTTGTA
    ACTAAACTTC AGAGAAGTTT GGCAGCACTT GTACACTCCA AAAAATCACC TAATGTCTGC
    TTGTCACTGC TTCAGGCTGA ACAGGGTAAA GTGCATCTGG GTGCTGTGCT TCAACTGCTC
    AGAACTGGAG ACAAAGGGAA AGCTGCCATC TTGTGGGAGG AGATAGAAGC AGGATTAGAG
    GCAGTGACAC CTAAAGGAGC ACTGACACCA GAACTTGGGC CTATCAGAGC TACTCTACTG
    GGGGCTAAAG CCGTAGCCTG CTGCTTGGCG TTGGCAATGA AGAAACAGTG TATACCCGAA
    GAGCTGTTTT CTTCATTGTG GCACTGGAAT CCACCAAAGG TTAAACCGCA ATTAAAATCT
    AAGCATCGAT CCAAGAGTCC GGACGCAGCT CCTCTAGAGG CAGGTGTTAA ACATAACCCT
    AAAGTTAAGA GTGGACTTGA GGAGAATGAA AGCAGAAAAA CCAAAGAGAC CACTGTCATT
    TCGAAGAAAG CTAAAGACTC TGTGCCCAAA ATTACCATCA CAAAATCCTC CATGGTTTTT
    AAAACGCCCA AAGCAACGAG AACAACCCGG CCCAAATCTG TCTCCACTAG CACTGGCATC
    AGTGACCTGA GAGCATTTGA CTTCAATAAT GAGGTGCCTG ACATTTCAGT CAACTTCACA
    CCATCATTAC ACCGATCTAC AAACCAAAGT GCGACTGCAA AGTCAAAAGC GTCCTCAAAG
    GGTTCGTTCC AGGTTTACAA AGATTCTTCT CCTGCTGCAG AGAAAGCTGT AGTTGTCCCG
    GCTGCTCCTA AACGAACAAA GCGTTCTCGC TTCAAGGTTG AGTTCAGCGA TGAAAGCGAC
    AACGATGCTG CTCCTCTTGC TGTAATGGAA AAGTCAGAGA AAAAGAAAAA CACCTTTAAA
    ACATCACGCA CCTTGAAACC AACCCTTAAC ACCATCCCTA GTGACACCAA ACTTGTGAGC
    TCCACAGTAG ATCCAGAACC CTCCAGACGA ACACGGACCA CTAAGAAAAG CACAGCTCTT
    CCCTCCATTG GTGGCCTCTC TTCTGAGGAA GAACTTGGAG TATCTTCACA GACACGTACG
    CGTAGAGGAC GACCAAAGAA GAGCACCAGC TCAGCAGACA CAGAGGAGCC AGAGAGGATG
    AGGATGATTA AGGAGGAAGA ACAGAGGGTT CTTTTGAATG TCAGCATGGA GGAGCTCAGA
    GGCTCTGACA CTGAGACAAA AGACACTGGA AGTCCAGATG CTGACTGTGA GGTCTTGAGG
    AGAGATCTAG GTGCAGATTT TGGGCGAGAG TGTTTCAGTG AGCTCAGGAG AGCTGGACAG
    AATGGATCAG GATCTCACAC AGCTCTGCCA CATGCAACTA CATCTCCTGC AGACTTGTCA
    GTAGAGACGG TCCAGTCATA CCTGCGCTCC TCATGGCTCC TCCTGCACCA CTTCCCGTTT
    CCTTCTCTTT ATCCTCACAT CTGCTCATTA CTCGCTCAGT CATTCGGTCA AACGGACCCC
    ATCATCACAG CAATGCTGCA TGCACAGGCA CTGGGAGTGT CCACTCGCCA CCATATGACC
    CGTCACGTGG TTAACCAGTT CAGAAAGTTG AAGAAATCCT GTAACGATGT AGCAGAAGGT
    CTTGGAGCTC TGAGTCTGGA GGAAACATCT GGAGCAACGC AGTCAAAGAA ACTCTCTGCG
    TTGGAGCAGA TCTTTTCATT CACAGCATCA AACCCAATGC AGTTTCCACA GACACACTGT
    CAGCAGTTCA CACAGCAGCT GGAAGATCTA CCTGCAGGAG TGACCGTATG CTTGCTGTCA
    CTGACGGGGG TGCATCCTGA TGAAATTGGC AACACTATTC TTCTGACCCG ACTGGAGCGC
    GGCTCTACCC CAATCACAGT TCGAATCCCC ACTGCAGACA GAGAGCAATC TGTTGCTGGC
    CTGTTGGAAG AAATGGATGG AGTCTTGAAA GGACAAAAGG AAGTGAGCAC AGTGGCTGAG
    AAATCACAGT GGTGGGACGG AAGGAAGGCT TTAGATGCCC GTGTGGAGAA AATGCTGGAG
    GAGATGGAAG AGGCTTTAGG AGTGTGGCGA ACTCTTTTTC TGCCTTTGAC CTCTGATCCT
    AAGCTTGATG TTCAGGTAAA GAGCCTCCAA AAGGCTCTTA AATCAACAAA GATCACACGG
    GACACTCTCA AGGTGATTCT ATCAGCGTCT CCACTGCTGT CCCTGCCAGA CCTGCAGTGT
    CTTCTGGAAG GAATGAATGT GCAGGACAAG GACCTGCTGA AGCTCCTGCA GAGAGGCGTG
    GCTGAACTGA GAGGGAGGGA GGAGCCACAG GGACACACTG TTCTGATTTT AGACAAGTAC
    TTGCAGAGGT TGCCGTGGGA GAACATTGCT TGTCTGAAGT CTCAGTCGGT CACTCGAATG
    CCCTCATTAC ACGCGGTGCT TGGACACAGT CATCTGAGAG AGGTGGATAC AAGTTGTGTT
    CTATCTTGTG GTGTGAACCC TAAGCAGGTT TACTATGTGC TAAACCCAGA TGGAAACCTG
    CCCGACACTG AGAATCGCTT CAAAGAGTGG TTTACAGGTG AACGAACATG GCAGGGGGTT
    TGTGGAAGGG CTCCTGATCC GGAGAAACTA CAAGAGGCTG TGACCACTAA AGATCTCTAC
    ATATACATCG GACATGGTGC AGGAGCCCGC TTTCTGGATG CGCAGAGAGT TTTGAAGGTG
    CCTGTGAGGA CCGCAGCTCT TCTGTTCGGC TGCAGTAGTG CTGCTCTCAG TGTGCTTGGT
    CAGCAGGAGG GAACGGGCAT CATCCTCAGC TACCTGACCG CTGGATGTCC ATTGGTGCTG
    GGTAACCTGT GGGATGTGAC AGATCGTGAC TTGGATCGAT TCACATCGGC TCTGCTCCAG
    TCTTGGCTCT CTGCTGGTTC TGGCTCTTCA CTTCTTCAGC ATTTGGCGAA ATCACGCAGC
    GCAACTCATT TAAAACACAT AATCGGTGCT GCGCCCATTG CGTATGGTTT ACCGGTGCAT
    ATACACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017212151.1
    CDS138..6686
    Translation

    Target ORF information:

    RefSeq Version XM_017356662.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio extra spindle pole bodies like 1, separase (espl1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017356662.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    ATGGCATTAA AGAGCTTGAA AACAGAAGAT TACGTTCAGC AGTTATCCTG TGTCAAGGAC 
    ACCATAGTCC TCCACAACGA ACTGAAGAAA CATGTGGATG GTGGCTTGGG TCCTTATGGT
    CGCACAATGT GTGATCGTAT CATCAGAGCC TGCAACCAGC GGCTGGGCAA CGGCGCTGTG
    GATGCTGGCC TTCAGGAGCG GATAGTGGAG CTGGTGGAAC TGGCTGTCCA AGGGTTTCAA
    CTGCTTGAAG AGTCCGGCAT GCAGAGCACC CCCTTTTATC TGGAGAAAAT AGTCTTTCAC
    ATCCTACAGA AAGTCGCAAG CCTTGGAGCT CATGGTCCTG CTTGTCGACT GGGTCAGCTC
    GTGTATAGAA GACTGAAATG TTTGTCTGCA GAGATTGATG ACTTTAATGT CCTGGTGCGG
    AATTGCTTTG CAGTCTTGTG GAACAGTCTC CTCTCTAACA AACCGAGTGC TTCCACCCTC
    CCTCCACGAG ACAGACTCCA CTGTCAGCTG CAAGCTTTAA CATTCAGACT CCTTGAACAG
    GGCTCCAGCT CGTCCTCTTC CCCATCCAAA GTTCCTCTGT TCGTGGAGGA AGCAGTAGTG
    GAGTACGAAC GATCATGTGG AGGTTTTACT CAAGACGATG TGAGTTTTCT GACCTCTGAA
    TTGCGTGAGC TGTTCTCTGG TGCTTTCATC AATCCTCAGG ACTCTGCATC AACTTCTTCA
    CTGCTGGCTG TGTGGTGTGA GGTGGCATTT AAAGTGTCTA AACTGCTCTG CAAAAGTAGC
    TTTTCACCTG AGGCTGTTGA ACTCTTGCGA GGCACTCTGA AGGAGGTTAA TGGCCACGTT
    GGCCTGCGTT CGGCTCTGGG TCTGGCAGAC CGGGCAGTGC AGATGCAGTG TGTGTTAAGT
    TCTGGAGGTG AATGCAGTAA GAGCTTCACT GAATGTGCCC GTGCTCTCCG GATGTTGCCT
    TGTGCCATGT CCAGCTCAGA GTCTCATGCC CTGTTAGAGG CTTGTCAGCT GGTGGTTTGG
    ACGTTGGAAG CCAGTCAGTG TAAAGGAATG GATGTGACCA CTTTGCTGGC TTGTTTTTCC
    TTTTTGGAAG AATATCAGGA ATTTCTGCTT GCACAGCAAA AGAACTCGTT TTCGCTGCAG
    GTCCAATACT CTCTTTGTTT CAGTTTTTAC CAGGGCTTCA TCTGCACATA TGATAGTCTT
    CATGCTTCTC AGGTGTCTGT GGGCGAGACC TTGGACAGAG TGCTATTGTT CTGCCGGTCG
    ACCGCAGGAC GTATGCTTGC TGAACTGCGG ACACTCAATA ATGACAACTT GCTGCTTAAA
    GCAGTGTGTG CAGTGAATAA TGTGGTGTAC GAGCTGTTTA ACAGGAAGCT GTATGAAGGA
    GCCTTTGGAT TGTCGATGAT TGTGTGTCAG GAGCTCTGTA AGGACTGCCC GCCCTCTCTT
    CCTATGGATC GGGTCAACCG CTGTTTCATG CTGACAGTGC AGTGTAGTCG CCGCGCAGGC
    CATCTGGAGC AGGCTCTGGA CTGGATTGTT CGCTGGATTC AGGCTTTGGG CTCACAGATT
    TTGGATCATC TCACTGAACC TGTGTCGATG TGGGTCAAAA CTAAGTGTGA TGCCGCCAGA
    GCAGGCGAGG ATGACACACG CCTGAGGACT CTGAGGGACG GGTTAGGAGA ATCCTTAGTG
    GATGAGGAGG TTATGATGTG TTTGTTAGAG GAGGAGTTGC GTGTATATAA GGAGCAGACT
    GGAGACACAG CACAGGAACG CTACAACACA CTCTGCGACC TCTTGGAAAT CTGCCATGAA
    GAAACCCCAC ACACACTAAG AAGAGCTACG TATCTGTGTG AGATGGCACA GGTTGTGTGC
    TATCAGGACT TCAGCCAGCA GACTGACTGC TCAGCAGTTG ATTTTACCCA CGAGGCTTTG
    CGGTTGCTGG AAGCAGAACC AGAGACGGCA GAGAATACTG ATAGGCTGAA GGATGAGAAA
    GCCCACGCTT CCCTGTGGCT CTATATCTGC ACTCTTGAGT CTAACCTGCA GGAGGCTGTG
    GACACAGAGA AGCGTTTGTG TGCAGTGCAG GAGGAGAATA AAAGAGATGT GAATATGGAT
    CCTGTTCCCA CAAATGATCT GGAGTACGAG GACAAACAGA AATCACAAGA GAGCCAGTTA
    GTCTACGATG GACTACGCTT CAACCTGCTG GCTCACAGCA AGCAAAGTGA ACCTCTAGAT
    AAATGTTTGT CTCTATGGCG GAGTCTCCTC AAGGGGGCTG TGCCTGCAGT TAGAGACCCT
    AAACTCACAG CTTCTTCCAT GACTTTGATG GCTGCCCTAT ACACACTTAT GGGCAAGCAC
    CTGCAAGCTC TGGAGGGATA TCAGCTTGCT GCCACCCTTT TCCATGTACT TGGTGATGGC
    CAGAGTAGTG CCAATGCCTT CTGCCACTCT ACCAGGATCT TGCTTTACCT GGGTTCACCG
    CAGCTTGCAC AGGTGGACTT AGAAAAAGCA GAAAAGAGCT TGTCATCTGT CACCATCTCT
    GAGGGCACAT CTATTATCTC CATGCTGGCC ATGTTGCAAA AAGCACAGCT ACACTATGCT
    CTAGGACAGG TGGAGAGTGG CGTGTCCTGC CTGATGGAGG TGATAAAGGA ATCTGCTCAG
    CATCATTCTA AGAGCTGGTA CCTGCTCAGG GCTCGAGCAC TGCAGACAGC CAGTGAATAC
    TTGAGTCTGG ACACACTGGC TTTGAACTCA CAGCTACGCC AGAGCATCAT GCAGCAGGGT
    CTAAAGACTC CAGACACTGC TCAATATGAA GGGTTGAAGC TGTTGTGTAG CCTGGTGATG
    ATGTTATTGG GCAACGGGTT TTACGGAGCG CCTGGACCAA ACACTGACAC TCGCTTTGTG
    GATAAAGGAG ACAGTGTTGT GTTTAAGTGG CTGCTGCTGA GTGAAGTGCT GGTGTGCTCG
    GAGAGAATGG TGATCACCAG GAGCAGCAGC GGAGCCGTTC ATGAAGCTAA AGCTCAGTGT
    CTAGAGGCTC TCAAACTGGC CACCAAACTA CAGACACTCA GCCACTGTGC TGAGCTGTTG
    GTGCTGAAGG CAGAGCTGGA GCTGATGAAG GGTGCAACTG AAGCTAGTAG TTTAGACCTA
    GAGCAAGTTA GACATTTACT TGATCTCTGC ACAGATTTTG GCCAGGGACG ACAACAGAAA
    TCTGAAGTGA AAATCAAACC ACGCAAAGGT CGTCCCGCTG CCCCTTCTTC CTCTGGTCAC
    ATCTCTGAGG AAGAGGATGA TCTGAGTGGG ATCCTCAGCT CTCGTGCCCT TCCGAAGGAG
    CCTGTGGAGA ACATGGCTAG ATTAGGAAGC CAAGGAGCTT CTCCCCCTCT AAAGCCCAAA
    TGCCAGCATG GCCTCTCCTG TCTGACCCAT GAAGAGACCT GCTCTTGTCT CTGCTGCACT
    GAGCTCAGCG TGGCTCGAGT CAGCATCCAC TGGGCACTAT TACAGGCAGA TCTCCAGACA
    GATCCCGAAC GCTCTCTCAG ACTACGCCAG TCTGCCAGGA AACGCTGCCG CTATGTTGTA
    ACTAAACTTC AGAGAAGTTT GGCAGCACTT GTACACTCCA AAAAATCACC TAATGTCTGC
    TTGTCACTGC TTCAGGCTGA ACAGGGTAAA GTGCATCTGG GTGCTGTGCT TCAACTGCTC
    AGAACTGGAG ACAAAGGGAA AGCTGCCATC TTGTGGGAGG AGATAGAAGC AGGATTAGAG
    GCAGTGACAC CTAAAGGAGC ACTGACACCA GAACTTGGGC CTATCAGAGC TACTCTACTG
    GGGGCTAAAG CCGTAGCCTG CTGCTTGGCG TTGGCAATGA AGAAACAGTG TATACCCGAA
    GAGCTGTTTT CTTCATTGTG GCACTGGAAT CCACCAAAGG TTAAACCGCA ATTAAAATCT
    AAGCATCGAT CCAAGAGTCC GGACGCAGCT CCTCTAGAGG CAGGTGTTAA ACATAACCCT
    AAAGTTAAGA GTGGACTTGA GGAGAATGAA AGCAGAAAAA CCAAAGAGAC CACTGTCATT
    TCGAAGAAAG CTAAAGACTC TGTGCCCAAA ATTACCATCA CAAAATCCTC CATGGTTTTT
    AAAACGCCCA AAGCAACGAG AACAACCCGG CCCAAATCTG TCTCCACTAG CACTGGCATC
    AGTGACCTGA GAGCATTTGA CTTCAATAAT GAGGTGCCTG ACATTTCAGT CAACTTCACA
    CCATCATTAC ACCGATCTAC AAACCAAAGT GCGACTGCAA AGTCAAAAGC GTCCTCAAAG
    GGTTCGTTCC AGGTTTACAA AGATTCTTCT CCTGCTGCAG AGAAAGCTGT AGTTGTCCCG
    GCTGCTCCTA AACGAACAAA GCGTTCTCGC TTCAAGGTTG AGTTCAGCGA TGAAAGCGAC
    AACGATGCTG CTCCTCTTGC TGTAATGGAA AAGTCAGAGA AAAAGAAAAA CACCTTTAAA
    ACATCACGCA CCTTGAAACC AACCCTTAAC ACCATCCCTA GTGACACCAA ACTTGTGAGC
    TCCACAGTAG ATCCAGAACC CTCCAGACGA ACACGGACCA CTAAGAAAAG CACAGCTCTT
    CCCTCCATTG GTGGCCTCTC TTCTGAGGAA GAACTTGGAG TATCTTCACA GACACGTACG
    CGTAGAGGAC GACCAAAGAA GAGCACCAGC TCAGCAGACA CAGAGGAGCC AGAGAGGATG
    AGGATGATTA AGGAGGAAGA ACAGAGGGTT CTTTTGAATG TCAGCATGGA GGAGCTCAGA
    GGCTCTGACA CTGAGACAAA AGACACTGGA AGTCCAGATG CTGACTGTGA GGTCTTGAGG
    AGAGATCTAG GTGCAGATTT TGGGCGAGAG TGTTTCAGTG AGCTCAGGAG AGCTGGACAG
    AATGGATCAG GATCTCACAC AGCTCTGCCA CATGCAACTA CATCTCCTGC AGACTTGTCA
    GTAGAGACGG TCCAGTCATA CCTGCGCTCC TCATGGCTCC TCCTGCACCA CTTCCCGTTT
    CCTTCTCTTT ATCCTCACAT CTGCTCATTA CTCGCTCAGT CATTCGGTCA AACGGACCCC
    ATCATCACAG CAATGCTGCA TGCACAGGCA CTGGGAGTGT CCACTCGCCA CCATATGACC
    CGTCACGTGG TTAACCAGTT CAGAAAGTTG AAGAAATCCT GTAACGATGT AGCAGAAGGT
    CTTGGAGCTC TGAGTCTGGA GGAAACATCT GGAGCAACGC AGTCAAAGAA ACTCTCTGCG
    TTGGAGCAGA TCTTTTCATT CACAGCATCA AACCCAATGC AGTTTCCACA GACACACTGT
    CAGCAGTTCA CACAGCAGCT GGAAGATCTA CCTGCAGGAG TGACCGTATG CTTGCTGTCA
    CTGACGGGGG TGCATCCTGA TGAAATTGGC AACACTATTC TTCTGACCCG ACTGGAGCGC
    GGCTCTACCC CAATCACAGT TCGAATCCCC ACTGCAGACA GAGAGCAATC TGTTGCTGGC
    CTGTTGGAAG AAATGGATGG AGTCTTGAAA GGACAAAAGG AAGTGAGCAC AGTGGCTGAG
    AAATCACAGT GGTGGGACGG AAGGAAGGCT TTAGATGCCC GTGTGGAGAA AATGCTGGAG
    GAGATGGAAG AGGCTTTAGG AGTGTGGCGA ACTCTTTTTC TGCCTTTGAC CTCTGATCCT
    AAGCTTGATG TTCAGGTAAA GAGCCTCCAA AAGGCTCTTA AATCAACAAA GATCACACGG
    GACACTCTCA AGGTGATTCT ATCAGCGTCT CCACTGCTGT CCCTGCCAGA CCTGCAGTGT
    CTTCTGGAAG GAATGAATGT GCAGGACAAG GACCTGCTGA AGCTCCTGCA GAGAGGCGTG
    GCTGAACTGA GAGGGAGGGA GGAGCCACAG GGACACACTG TTCTGATTTT AGACAAGTAC
    TTGCAGAGGT TGCCGTGGGA GAACATTGCT TGTCTGAAGT CTCAGTCGGT CACTCGAATG
    CCCTCATTAC ACGCGGTGCT TGGACACAGT CATCTGAGAG AGGTGGATAC AAGTTGTGTT
    CTATCTTGTG GTGTGAACCC TAAGCAGGTT TACTATGTGC TAAACCCAGA TGGAAACCTG
    CCCGACACTG AGAATCGCTT CAAAGAGTGG TTTACAGGTG AACGAACATG GCAGGGGGTT
    TGTGGAAGGG CTCCTGATCC GGAGAAACTA CAAGAGGCTG TGACCACTAA AGATCTCTAC
    ATATACATCG GACATGGTGC AGGAGCCCGC TTTCTGGATG CGCAGAGAGT TTTGAAGGTG
    CCTGTGAGGA CCGCAGCTCT TCTGTTCGGC TGCAGTAGTG CTGCTCTCAG TGTGCTTGGT
    CAGCAGGAGG GAACGGGCAT CATCCTCAGC TACCTGACCG CTGGATGTCC ATTGGTGCTG
    GGTAACCTGT GGGATGTGAC AGATCGTGAC TTGGATCGAT TCACATCGGC TCTGCTCCAG
    TCTTGGCTCT CTGCTGGTTC TGGCTCTTCA CTTCTTCAGC ATTTGGCGAA ATCACGCAGC
    GCAACTCATT TAAAACACAT AATCGGTGCT GCGCCCATTG CGTATGGTTT ACCGGTGCAT
    ATACACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa12113
    Clone ID Related Accession (Same CDS sequence) XM_005166094.3 , XM_021477221.1 , XM_005166094.4
    Accession Version XM_005166094.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6546bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product separin isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005166094.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    ATGGCATTAA AGAGCTTGAA AACAGAAGAT TACGTTCAGC AGTTATCCTG TGTCAAGGAC 
    ACCATAGTCC TCCACAACGA ACTGAAGAAA CATGTGGATG GTGGCTTGGG TCCTTATGGT
    CGCACAATGT GTGATCGTAT CATCAGAGCC TGCAACCAGC GGCTGGGCAA CGGCGCTGTG
    GATGCTGGCC TTCAGGAGCG GATAGTGGAG CTGGTGGAAC TGGCTGTCCA AGGGTTTCAA
    CTGCTTGAAG AGTCCGGCAT GCAGAGCACC CCCTTTTATC TGGAGAAAAT AGTCTTTCAC
    ATCCTACAGA AAGTCGCAAG CCTTGGAGCT CATGGTCCTG CTTGTCGACT GGGTCAGCTC
    GTGTATAGAA GACTGAAATG TTTGTCTGCA GAGATTGATG ACTTTAATGT CCTGGTGCGG
    AATTGCTTTG CAGTCTTGTG GAACAGTCTC CTCTCTAACA AACCGAGTGC TTCCACCCTC
    CCTCCACGAG ACAGACTCCA CTGTCAGCTG CAAGCTTTAA CATTCAGACT CCTTGAACAG
    GGCTCCAGCT CGTCCTCTTC CCCATCCAAA GTTCCTCTGT TCGTGGAGGA AGCAGTAGTG
    GAGTACGAAC GATCATGTGG AGGTTTTACT CAAGACGATG TGAGTTTTCT GACCTCTGAA
    TTGCGTGAGC TGTTCTCTGG TGCTTTCATC AATCCTCAGG ACTCTGCATC AACTTCTTCA
    CTGCTGGCTG TGTGGTGTGA GGTGGCATTT AAAGTGTCTA AACTGCTCTG CAAAAGTAGC
    TTTTCACCTG AGGCTGTTGA ACTCTTGCGA GGCACTCTGA AGGAGGTTAA TGGCCACGTT
    GGCCTGCGTT CGGCTCTGGG TCTGGCAGAC CGGGCAGTGC AGATGCAGTG TGTGTTAAGT
    TCTGGAGGTG AATGCAGTAA GAGCTTCACT GAATGTGCCC GTGCTCTCCG GATGTTGCCT
    TGTGCCATGT CCAGCTCAGA GTCTCATGCC CTGTTAGAGG CTTGTCAGCT GGTGGTTTGG
    ACGTTGGAAG CCAGTCAGTG TAAAGGAATG GATGTGACCA CTTTGCTGGC TTGTTTTTCC
    TTTTTGGAAG AATATCAGGA ATTTCTGCTT GCACAGCAAA AGAACTCGTT TTCGCTGCAG
    GTCCAATACT CTCTTTGTTT CAGTTTTTAC CAGGGCTTCA TCTGCACATA TGATAGTCTT
    CATGCTTCTC AGGTGTCTGT GGGCGAGACC TTGGACAGAG TGCTATTGTT CTGCCGGTCG
    ACCGCAGGAC GTATGCTTGC TGAACTGCGG ACACTCAATA ATGACAACTT GCTGCTTAAA
    GCAGTGTGTG CAGTGAATAA TGTGGTGTAC GAGCTGTTTA ACAGGAAGCT GTATGAAGGA
    GCCTTTGGAT TGTCGATGAT TGTGTGTCAG GAGCTCTGTA AGGACTGCCC GCCCTCTCTT
    CCTATGGATC GGGTCAACCG CTGTTTCATG CTGACAGTGC AGTGTAGTCG CCGCGCAGGC
    CATCTGGAGC AGGCTCTGGA CTGGATTGTT CGCTGGATTC AGGCTTTGGG CTCACAGATT
    TTGGATCATC TCACTGAACC TGTGTCGATG TGGGTCAAAA CTAAGTGTGA TGCCGCCAGA
    GCAGGCGAGG ATGACACACG CCTGAGGACT CTGAGGGACG GGTTAGGAGA ATCCTTAGTG
    GATGAGGAGG TTATGATGTG TTTGTTAGAG GAGGAGTTGC GTGTATATAA GGAGCAGACT
    GGAGACACAG CACAGGAACG CTACAACACA CTCTGCGACC TCTTGGAAAT CTGCCATGAA
    GAAACCCCAC ACACACTAAG AAGAGCTACG TATCTGTGTG AGATGGCACA GGTTGTGTGC
    TATCAGGACT TCAGCCAGCA GACTGACTGC TCAGCAGTTG ATTTTACCCA CGAGGCTTTG
    CGGTTGCTGG AAGCAGAACC AGAGACGGCA GAGAATACTG ATAGGCTGAA GGATGAGAAA
    GCCCACGCTT CCCTGTGGCT CTATATCTGC ACTCTTGAGT CTAACCTGCA GGAGGCTGTG
    GACACAGAGA AGCGTTTGTG TGCAGTGCAG GAGGAGAATA AAAGAGATGT GAATATGGAT
    CCTGTTCCCA CAAATGATCT GGAGTACGAG GACAAACAGA AATCACAAGA GAGCCAGTTA
    GTCTACGATG GACTACGCTT CAACCTGCTG GCTCACAGCA AGCAAAGTGA ACCTCTAGAT
    AAATGTTTGT CTCTATGGCG GAGTCTCCTC AAGGGGGCTG TGCCTGCAGT TAGAGACCCT
    AAACTCACAG CTTCTTCCAT GACTTTGATG GCTGCCCTAT ACACACTTAT GGGCAAGCAC
    CTGCAAGCTC TGGAGGGATA TCAGCTTGCT GCCACCCTTT TCCATGTACT TGGTGATGGC
    CAGAGTAGTG CCAATGCCTT CTGCCACTCT ACCAGGATCT TGCTTTACCT GGGTTCACCG
    CAGCTTGCAC AGGTGGACTT AGAAAAAGCA GAAAAGAGCT TGTCATCTGT CACCATCTCT
    GAGGGCACAT CTATTATCTC CATGCTGGCC ATGTTGCAAA AAGCACAGCT ACACTATGCT
    CTAGGACAGG TGGAGAGTGG CGTGTCCTGC CTGATGGAGG TGATAAAGGA ATCTGCTCAG
    CATCATTCTA AGAGCTGGTA CCTGCTCAGG GCTCGAGCAC TGCAGACAGC CAGTGAATAC
    TTGAGTCTGG ACACACTGGC TTTGAACTCA CAGCTACGCC AGAGCATCAT GCAGCAGGGT
    CTAAAGACTC CAGACACTGC TCAATATGAA GGGTTGAAGC TGTTGTGTAG CCTGGTGATG
    ATGTTATTGG GCAACGGGTT TTACGGAGCG CCTGGACCAA ACACTGACAC TCGCTTTGTG
    GATAAAGGAG ACAGTGTTGT GTTTAAGTGG CTGCTGCTGA GTGAAGTGCT GGTGTGCTCG
    GAGAGAATGG TGATCACCAG GAGCAGCAGC GGAGCCGTTC ATGAAGCTAA AGCTCAGTGT
    CTAGAGGCTC TCAAACTGGC CACCAAACTA CAGACACTCA GCCACTGTGC TGAGCTGTTG
    GTGCTGAAGG CAGAGCTGGA GCTGATGAAG GGTGCAACTG AAGCTAGTAG TTTAGACCTA
    GAGCAAGTTA GACATTTACT TGATCTCTGC ACAGATTTTG GCCAGGGACG ACAACAGAAA
    TCTGAAGTGA AAATCAAACC ACGCAAAGGT CGTCCCGCTG CCCCTTCTTC CTCTGGTCAC
    ATCTCTGAGG AAGAGGATGA TCTGAGTGGG ATCCTCAGCT CTCGTGCCCT TCCGAAGGAG
    CCTGTGGAGA ACATGGCTAG ATTAGGAAGC CAAGGAGCTT CTCCCCCTCT AAAGCCCAAA
    TGCCAGCATG GCCTCTCCTG TCTGACCCAT GAAGAGACCT GCTCTTGTCT CTGCTGCACT
    GAGCTCAGCG TGGCTCGAGT CAGCATCCAC TGGGCACTAT TACAGGCAGA TCTCCAGACA
    GATCCCGAAC GCTCTCTCAG ACTACGCCAG TCTGCCAGGA AACGCTGCCG CTATGTTGTA
    ACTAAACTTC AGAGAAGTTT GGCAGCACTT GTACACTCCA AAAAATCACC TAATGTCTGC
    TTGTCACTGC TTCAGGCTGA ACAGGGTAAA GTGCATCTGG GTGCTGTGCT TCAACTGCTC
    AGAACTGGAG ACAAAGGGAA AGCTGCCATC TTGTGGGAGG AGATAGAAGC AGGATTAGAG
    GCAGTGACAC CTAAAGGAGC ACTGACACCA GAACTTGGGC CTATCAGAGC TACTCTACTG
    GGGGCTAAAG CCGTAGCCTG CTGCTTGGCG TTGGCAATGA AGAAACAGTG TATACCCGAA
    GAGCTGTTTT CTTCATTGTG GCACTGGAAT CCACCAAAGG TTAAACCGCA ATTAAAATCT
    AAGCATCGAT CCAAGAGTCC GGACGCAGCT CCTCTAGAGG CAGGTGTTAA ACATAACCCT
    AAAGTTAAGA GTGGACTTGA GGAGAATGAA AGCAGAAAAA CCAAAGAGAC CACTGTCATT
    TCGAAGAAAG CTAAAGACTC TGTGCCCAAA ATTACCATCA CAAAATCCTC CATGGTTTTT
    AAAACGCCCA AAGCAACGAG AACAACCCGG CCCAAATCTG TCTCCACTAG CACTGGCATC
    AGTGACCTGA GAGCATTTGA CTTCAATAAT GAGGTGCCTG ACATTTCAGT CAACTTCACA
    CCATCATTAC ACCGATCTAC AAACCAAAGT GCGACTGCAA AGTCAAAAGC GTCCTCAAAG
    GGTTCGTTCC AGGTTTACAA AGATTCTTCT CCTGCTGCAG AGAAAGCTGT AGTTGTCCCG
    GCTGCTCCTA AACGAACAAA GCGTTCTCGC TTCAAGGTTG AGTTCAGCGA TGAAAGCGAC
    AACGATGCTG CTCCTCTTGC TGTAATGGAA AAGTCAGAGA AAAAGAAAAA CACCTTTAAA
    ACATCACGCA CCTTGAAACC AACCCTTAAC ACCATCCCTA GTGACACCAA ACTTGTGAGC
    TCCACAGTAG ATCCAGAACC CTCCAGACGA ACACGGACCA CTAAGAAAAG CACAGCTCTT
    CCCTCCATTG GTGGCCTCTC TTCTGAGGAA GAACTTGGAG TATCTTCACA GACACGTACG
    CGTAGAGGAC GACCAAAGAA GAGCACCAGC TCAGCAGACA CAGAGGAGCC AGAGAGGATG
    AGGATGATTA AGGAGGAAGA ACAGAGGGTT CTTTTGAATG TCAGCATGGA GGAGCTCAGA
    GGCTCTGACA CTGAGACAAA AGACACTGGA AGTCCAGATG CTGACTGTGA GGTCTTGAGG
    AGAGATCTAG GTGCAGATTT TGGGCGAGAG TGTTTCAGTG AGCTCAGGAG AGCTGGACAG
    AATGGATCAG GATCTCACAC AGCTCTGCCA CATGCAACTA CATCTCCTGA CTTGTCAGTA
    GAGACGGTCC AGTCATACCT GCGCTCCTCA TGGCTCCTCC TGCACCACTT CCCGTTTCCT
    TCTCTTTATC CTCACATCTG CTCATTACTC GCTCAGTCAT TCGGTCAAAC GGACCCCATC
    ATCACAGCAA TGCTGCATGC ACAGGCACTG GGAGTGTCCA CTCGCCACCA TATGACCCGT
    CACGTGGTTA ACCAGTTCAG AAAGTTGAAG AAATCCTGTA ACGATGTAGC AGAAGGTCTT
    GGAGCTCTGA GTCTGGAGGA AACATCTGGA GCAACGCAGT CAAAGAAACT CTCTGCGTTG
    GAGCAGATCT TTTCATTCAC AGCATCAAAC CCAATGCAGT TTCCACAGAC ACACTGTCAG
    CAGTTCACAC AGCAGCTGGA AGATCTACCT GCAGGAGTGA CCGTATGCTT GCTGTCACTG
    ACGGGGGTGC ATCCTGATGA AATTGGCAAC ACTATTCTTC TGACCCGACT GGAGCGCGGC
    TCTACCCCAA TCACAGTTCG AATCCCCACT GCAGACAGAG AGCAATCTGT TGCTGGCCTG
    TTGGAAGAAA TGGATGGAGT CTTGAAAGGA CAAAAGGAAG TGAGCACAGT GGCTGAGAAA
    TCACAGTGGT GGGACGGAAG GAAGGCTTTA GATGCCCGTG TGGAGAAAAT GCTGGAGGAG
    ATGGAAGAGG CTTTAGGAGT GTGGCGAACT CTTTTTCTGC CTTTGACCTC TGATCCTAAG
    CTTGATGTTC AGGTAAAGAG CCTCCAAAAG GCTCTTAAAT CAACAAAGAT CACACGGGAC
    ACTCTCAAGG TGATTCTATC AGCGTCTCCA CTGCTGTCCC TGCCAGACCT GCAGTGTCTT
    CTGGAAGGAA TGAATGTGCA GGACAAGGAC CTGCTGAAGC TCCTGCAGAG AGGCGTGGCT
    GAACTGAGAG GGAGGGAGGA GCCACAGGGA CACACTGTTC TGATTTTAGA CAAGTACTTG
    CAGAGGTTGC CGTGGGAGAA CATTGCTTGT CTGAAGTCTC AGTCGGTCAC TCGAATGCCC
    TCATTACACG CGGTGCTTGG ACACAGTCAT CTGAGAGAGG TGGATACAAG TTGTGTTCTA
    TCTTGTGGTG TGAACCCTAA GCAGGTTTAC TATGTGCTAA ACCCAGATGG AAACCTGCCC
    GACACTGAGA ATCGCTTCAA AGAGTGGTTT ACAGGTGAAC GAACATGGCA GGGGGTTTGT
    GGAAGGGCTC CTGATCCGGA GAAACTACAA GAGGCTGTGA CCACTAAAGA TCTCTACATA
    TACATCGGAC ATGGTGCAGG AGCCCGCTTT CTGGATGCGC AGAGAGTTTT GAAGGTGCCT
    GTGAGGACCG CAGCTCTTCT GTTCGGCTGC AGTAGTGCTG CTCTCAGTGT GCTTGGTCAG
    CAGGAGGGAA CGGGCATCAT CCTCAGCTAC CTGACCGCTG GATGTCCATT GGTGCTGGGT
    AACCTGTGGG ATGTGACAGA TCGTGACTTG GATCGATTCA CATCGGCTCT GCTCCAGTCT
    TGGCTCTCTG CTGGTTCTGG CTCTTCACTT CTTCAGCATT TGGCGAAATC ACGCAGCGCA
    ACTCATTTAA AACACATAAT CGGTGCTGCG CCCATTGCGT ATGGTTTACC GGTGCATATA
    CACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005166151.1
    CDS187..6732
    Translation

    Target ORF information:

    RefSeq Version XM_005166094.3
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio extra spindle pole bodies like 1, separase (espl1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005166094.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    ATGGCATTAA AGAGCTTGAA AACAGAAGAT TACGTTCAGC AGTTATCCTG TGTCAAGGAC 
    ACCATAGTCC TCCACAACGA ACTGAAGAAA CATGTGGATG GTGGCTTGGG TCCTTATGGT
    CGCACAATGT GTGATCGTAT CATCAGAGCC TGCAACCAGC GGCTGGGCAA CGGCGCTGTG
    GATGCTGGCC TTCAGGAGCG GATAGTGGAG CTGGTGGAAC TGGCTGTCCA AGGGTTTCAA
    CTGCTTGAAG AGTCCGGCAT GCAGAGCACC CCCTTTTATC TGGAGAAAAT AGTCTTTCAC
    ATCCTACAGA AAGTCGCAAG CCTTGGAGCT CATGGTCCTG CTTGTCGACT GGGTCAGCTC
    GTGTATAGAA GACTGAAATG TTTGTCTGCA GAGATTGATG ACTTTAATGT CCTGGTGCGG
    AATTGCTTTG CAGTCTTGTG GAACAGTCTC CTCTCTAACA AACCGAGTGC TTCCACCCTC
    CCTCCACGAG ACAGACTCCA CTGTCAGCTG CAAGCTTTAA CATTCAGACT CCTTGAACAG
    GGCTCCAGCT CGTCCTCTTC CCCATCCAAA GTTCCTCTGT TCGTGGAGGA AGCAGTAGTG
    GAGTACGAAC GATCATGTGG AGGTTTTACT CAAGACGATG TGAGTTTTCT GACCTCTGAA
    TTGCGTGAGC TGTTCTCTGG TGCTTTCATC AATCCTCAGG ACTCTGCATC AACTTCTTCA
    CTGCTGGCTG TGTGGTGTGA GGTGGCATTT AAAGTGTCTA AACTGCTCTG CAAAAGTAGC
    TTTTCACCTG AGGCTGTTGA ACTCTTGCGA GGCACTCTGA AGGAGGTTAA TGGCCACGTT
    GGCCTGCGTT CGGCTCTGGG TCTGGCAGAC CGGGCAGTGC AGATGCAGTG TGTGTTAAGT
    TCTGGAGGTG AATGCAGTAA GAGCTTCACT GAATGTGCCC GTGCTCTCCG GATGTTGCCT
    TGTGCCATGT CCAGCTCAGA GTCTCATGCC CTGTTAGAGG CTTGTCAGCT GGTGGTTTGG
    ACGTTGGAAG CCAGTCAGTG TAAAGGAATG GATGTGACCA CTTTGCTGGC TTGTTTTTCC
    TTTTTGGAAG AATATCAGGA ATTTCTGCTT GCACAGCAAA AGAACTCGTT TTCGCTGCAG
    GTCCAATACT CTCTTTGTTT CAGTTTTTAC CAGGGCTTCA TCTGCACATA TGATAGTCTT
    CATGCTTCTC AGGTGTCTGT GGGCGAGACC TTGGACAGAG TGCTATTGTT CTGCCGGTCG
    ACCGCAGGAC GTATGCTTGC TGAACTGCGG ACACTCAATA ATGACAACTT GCTGCTTAAA
    GCAGTGTGTG CAGTGAATAA TGTGGTGTAC GAGCTGTTTA ACAGGAAGCT GTATGAAGGA
    GCCTTTGGAT TGTCGATGAT TGTGTGTCAG GAGCTCTGTA AGGACTGCCC GCCCTCTCTT
    CCTATGGATC GGGTCAACCG CTGTTTCATG CTGACAGTGC AGTGTAGTCG CCGCGCAGGC
    CATCTGGAGC AGGCTCTGGA CTGGATTGTT CGCTGGATTC AGGCTTTGGG CTCACAGATT
    TTGGATCATC TCACTGAACC TGTGTCGATG TGGGTCAAAA CTAAGTGTGA TGCCGCCAGA
    GCAGGCGAGG ATGACACACG CCTGAGGACT CTGAGGGACG GGTTAGGAGA ATCCTTAGTG
    GATGAGGAGG TTATGATGTG TTTGTTAGAG GAGGAGTTGC GTGTATATAA GGAGCAGACT
    GGAGACACAG CACAGGAACG CTACAACACA CTCTGCGACC TCTTGGAAAT CTGCCATGAA
    GAAACCCCAC ACACACTAAG AAGAGCTACG TATCTGTGTG AGATGGCACA GGTTGTGTGC
    TATCAGGACT TCAGCCAGCA GACTGACTGC TCAGCAGTTG ATTTTACCCA CGAGGCTTTG
    CGGTTGCTGG AAGCAGAACC AGAGACGGCA GAGAATACTG ATAGGCTGAA GGATGAGAAA
    GCCCACGCTT CCCTGTGGCT CTATATCTGC ACTCTTGAGT CTAACCTGCA GGAGGCTGTG
    GACACAGAGA AGCGTTTGTG TGCAGTGCAG GAGGAGAATA AAAGAGATGT GAATATGGAT
    CCTGTTCCCA CAAATGATCT GGAGTACGAG GACAAACAGA AATCACAAGA GAGCCAGTTA
    GTCTACGATG GACTACGCTT CAACCTGCTG GCTCACAGCA AGCAAAGTGA ACCTCTAGAT
    AAATGTTTGT CTCTATGGCG GAGTCTCCTC AAGGGGGCTG TGCCTGCAGT TAGAGACCCT
    AAACTCACAG CTTCTTCCAT GACTTTGATG GCTGCCCTAT ACACACTTAT GGGCAAGCAC
    CTGCAAGCTC TGGAGGGATA TCAGCTTGCT GCCACCCTTT TCCATGTACT TGGTGATGGC
    CAGAGTAGTG CCAATGCCTT CTGCCACTCT ACCAGGATCT TGCTTTACCT GGGTTCACCG
    CAGCTTGCAC AGGTGGACTT AGAAAAAGCA GAAAAGAGCT TGTCATCTGT CACCATCTCT
    GAGGGCACAT CTATTATCTC CATGCTGGCC ATGTTGCAAA AAGCACAGCT ACACTATGCT
    CTAGGACAGG TGGAGAGTGG CGTGTCCTGC CTGATGGAGG TGATAAAGGA ATCTGCTCAG
    CATCATTCTA AGAGCTGGTA CCTGCTCAGG GCTCGAGCAC TGCAGACAGC CAGTGAATAC
    TTGAGTCTGG ACACACTGGC TTTGAACTCA CAGCTACGCC AGAGCATCAT GCAGCAGGGT
    CTAAAGACTC CAGACACTGC TCAATATGAA GGGTTGAAGC TGTTGTGTAG CCTGGTGATG
    ATGTTATTGG GCAACGGGTT TTACGGAGCG CCTGGACCAA ACACTGACAC TCGCTTTGTG
    GATAAAGGAG ACAGTGTTGT GTTTAAGTGG CTGCTGCTGA GTGAAGTGCT GGTGTGCTCG
    GAGAGAATGG TGATCACCAG GAGCAGCAGC GGAGCCGTTC ATGAAGCTAA AGCTCAGTGT
    CTAGAGGCTC TCAAACTGGC CACCAAACTA CAGACACTCA GCCACTGTGC TGAGCTGTTG
    GTGCTGAAGG CAGAGCTGGA GCTGATGAAG GGTGCAACTG AAGCTAGTAG TTTAGACCTA
    GAGCAAGTTA GACATTTACT TGATCTCTGC ACAGATTTTG GCCAGGGACG ACAACAGAAA
    TCTGAAGTGA AAATCAAACC ACGCAAAGGT CGTCCCGCTG CCCCTTCTTC CTCTGGTCAC
    ATCTCTGAGG AAGAGGATGA TCTGAGTGGG ATCCTCAGCT CTCGTGCCCT TCCGAAGGAG
    CCTGTGGAGA ACATGGCTAG ATTAGGAAGC CAAGGAGCTT CTCCCCCTCT AAAGCCCAAA
    TGCCAGCATG GCCTCTCCTG TCTGACCCAT GAAGAGACCT GCTCTTGTCT CTGCTGCACT
    GAGCTCAGCG TGGCTCGAGT CAGCATCCAC TGGGCACTAT TACAGGCAGA TCTCCAGACA
    GATCCCGAAC GCTCTCTCAG ACTACGCCAG TCTGCCAGGA AACGCTGCCG CTATGTTGTA
    ACTAAACTTC AGAGAAGTTT GGCAGCACTT GTACACTCCA AAAAATCACC TAATGTCTGC
    TTGTCACTGC TTCAGGCTGA ACAGGGTAAA GTGCATCTGG GTGCTGTGCT TCAACTGCTC
    AGAACTGGAG ACAAAGGGAA AGCTGCCATC TTGTGGGAGG AGATAGAAGC AGGATTAGAG
    GCAGTGACAC CTAAAGGAGC ACTGACACCA GAACTTGGGC CTATCAGAGC TACTCTACTG
    GGGGCTAAAG CCGTAGCCTG CTGCTTGGCG TTGGCAATGA AGAAACAGTG TATACCCGAA
    GAGCTGTTTT CTTCATTGTG GCACTGGAAT CCACCAAAGG TTAAACCGCA ATTAAAATCT
    AAGCATCGAT CCAAGAGTCC GGACGCAGCT CCTCTAGAGG CAGGTGTTAA ACATAACCCT
    AAAGTTAAGA GTGGACTTGA GGAGAATGAA AGCAGAAAAA CCAAAGAGAC CACTGTCATT
    TCGAAGAAAG CTAAAGACTC TGTGCCCAAA ATTACCATCA CAAAATCCTC CATGGTTTTT
    AAAACGCCCA AAGCAACGAG AACAACCCGG CCCAAATCTG TCTCCACTAG CACTGGCATC
    AGTGACCTGA GAGCATTTGA CTTCAATAAT GAGGTGCCTG ACATTTCAGT CAACTTCACA
    CCATCATTAC ACCGATCTAC AAACCAAAGT GCGACTGCAA AGTCAAAAGC GTCCTCAAAG
    GGTTCGTTCC AGGTTTACAA AGATTCTTCT CCTGCTGCAG AGAAAGCTGT AGTTGTCCCG
    GCTGCTCCTA AACGAACAAA GCGTTCTCGC TTCAAGGTTG AGTTCAGCGA TGAAAGCGAC
    AACGATGCTG CTCCTCTTGC TGTAATGGAA AAGTCAGAGA AAAAGAAAAA CACCTTTAAA
    ACATCACGCA CCTTGAAACC AACCCTTAAC ACCATCCCTA GTGACACCAA ACTTGTGAGC
    TCCACAGTAG ATCCAGAACC CTCCAGACGA ACACGGACCA CTAAGAAAAG CACAGCTCTT
    CCCTCCATTG GTGGCCTCTC TTCTGAGGAA GAACTTGGAG TATCTTCACA GACACGTACG
    CGTAGAGGAC GACCAAAGAA GAGCACCAGC TCAGCAGACA CAGAGGAGCC AGAGAGGATG
    AGGATGATTA AGGAGGAAGA ACAGAGGGTT CTTTTGAATG TCAGCATGGA GGAGCTCAGA
    GGCTCTGACA CTGAGACAAA AGACACTGGA AGTCCAGATG CTGACTGTGA GGTCTTGAGG
    AGAGATCTAG GTGCAGATTT TGGGCGAGAG TGTTTCAGTG AGCTCAGGAG AGCTGGACAG
    AATGGATCAG GATCTCACAC AGCTCTGCCA CATGCAACTA CATCTCCTGA CTTGTCAGTA
    GAGACGGTCC AGTCATACCT GCGCTCCTCA TGGCTCCTCC TGCACCACTT CCCGTTTCCT
    TCTCTTTATC CTCACATCTG CTCATTACTC GCTCAGTCAT TCGGTCAAAC GGACCCCATC
    ATCACAGCAA TGCTGCATGC ACAGGCACTG GGAGTGTCCA CTCGCCACCA TATGACCCGT
    CACGTGGTTA ACCAGTTCAG AAAGTTGAAG AAATCCTGTA ACGATGTAGC AGAAGGTCTT
    GGAGCTCTGA GTCTGGAGGA AACATCTGGA GCAACGCAGT CAAAGAAACT CTCTGCGTTG
    GAGCAGATCT TTTCATTCAC AGCATCAAAC CCAATGCAGT TTCCACAGAC ACACTGTCAG
    CAGTTCACAC AGCAGCTGGA AGATCTACCT GCAGGAGTGA CCGTATGCTT GCTGTCACTG
    ACGGGGGTGC ATCCTGATGA AATTGGCAAC ACTATTCTTC TGACCCGACT GGAGCGCGGC
    TCTACCCCAA TCACAGTTCG AATCCCCACT GCAGACAGAG AGCAATCTGT TGCTGGCCTG
    TTGGAAGAAA TGGATGGAGT CTTGAAAGGA CAAAAGGAAG TGAGCACAGT GGCTGAGAAA
    TCACAGTGGT GGGACGGAAG GAAGGCTTTA GATGCCCGTG TGGAGAAAAT GCTGGAGGAG
    ATGGAAGAGG CTTTAGGAGT GTGGCGAACT CTTTTTCTGC CTTTGACCTC TGATCCTAAG
    CTTGATGTTC AGGTAAAGAG CCTCCAAAAG GCTCTTAAAT CAACAAAGAT CACACGGGAC
    ACTCTCAAGG TGATTCTATC AGCGTCTCCA CTGCTGTCCC TGCCAGACCT GCAGTGTCTT
    CTGGAAGGAA TGAATGTGCA GGACAAGGAC CTGCTGAAGC TCCTGCAGAG AGGCGTGGCT
    GAACTGAGAG GGAGGGAGGA GCCACAGGGA CACACTGTTC TGATTTTAGA CAAGTACTTG
    CAGAGGTTGC CGTGGGAGAA CATTGCTTGT CTGAAGTCTC AGTCGGTCAC TCGAATGCCC
    TCATTACACG CGGTGCTTGG ACACAGTCAT CTGAGAGAGG TGGATACAAG TTGTGTTCTA
    TCTTGTGGTG TGAACCCTAA GCAGGTTTAC TATGTGCTAA ACCCAGATGG AAACCTGCCC
    GACACTGAGA ATCGCTTCAA AGAGTGGTTT ACAGGTGAAC GAACATGGCA GGGGGTTTGT
    GGAAGGGCTC CTGATCCGGA GAAACTACAA GAGGCTGTGA CCACTAAAGA TCTCTACATA
    TACATCGGAC ATGGTGCAGG AGCCCGCTTT CTGGATGCGC AGAGAGTTTT GAAGGTGCCT
    GTGAGGACCG CAGCTCTTCT GTTCGGCTGC AGTAGTGCTG CTCTCAGTGT GCTTGGTCAG
    CAGGAGGGAA CGGGCATCAT CCTCAGCTAC CTGACCGCTG GATGTCCATT GGTGCTGGGT
    AACCTGTGGG ATGTGACAGA TCGTGACTTG GATCGATTCA CATCGGCTCT GCTCCAGTCT
    TGGCTCTCTG CTGGTTCTGG CTCTTCACTT CTTCAGCATT TGGCGAAATC ACGCAGCGCA
    ACTCATTTAA AACACATAAT CGGTGCTGCG CCCATTGCGT ATGGTTTACC GGTGCATATA
    CACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa12113
    Clone ID Related Accession (Same CDS sequence) XM_005166094.3 , XM_021477221.1 , XM_005166094.4
    Accession Version XM_021477221.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6546bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product separin isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    ATGGCATTAA AGAGCTTGAA AACAGAAGAT TACGTTCAGC AGTTATCCTG TGTCAAGGAC 
    ACCATAGTCC TCCACAACGA ACTGAAGAAA CATGTGGATG GTGGCTTGGG TCCTTATGGT
    CGCACAATGT GTGATCGTAT CATCAGAGCC TGCAACCAGC GGCTGGGCAA CGGCGCTGTG
    GATGCTGGCC TTCAGGAGCG GATAGTGGAG CTGGTGGAAC TGGCTGTCCA AGGGTTTCAA
    CTGCTTGAAG AGTCCGGCAT GCAGAGCACC CCCTTTTATC TGGAGAAAAT AGTCTTTCAC
    ATCCTACAGA AAGTCGCAAG CCTTGGAGCT CATGGTCCTG CTTGTCGACT GGGTCAGCTC
    GTGTATAGAA GACTGAAATG TTTGTCTGCA GAGATTGATG ACTTTAATGT CCTGGTGCGG
    AATTGCTTTG CAGTCTTGTG GAACAGTCTC CTCTCTAACA AACCGAGTGC TTCCACCCTC
    CCTCCACGAG ACAGACTCCA CTGTCAGCTG CAAGCTTTAA CATTCAGACT CCTTGAACAG
    GGCTCCAGCT CGTCCTCTTC CCCATCCAAA GTTCCTCTGT TCGTGGAGGA AGCAGTAGTG
    GAGTACGAAC GATCATGTGG AGGTTTTACT CAAGACGATG TGAGTTTTCT GACCTCTGAA
    TTGCGTGAGC TGTTCTCTGG TGCTTTCATC AATCCTCAGG ACTCTGCATC AACTTCTTCA
    CTGCTGGCTG TGTGGTGTGA GGTGGCATTT AAAGTGTCTA AACTGCTCTG CAAAAGTAGC
    TTTTCACCTG AGGCTGTTGA ACTCTTGCGA GGCACTCTGA AGGAGGTTAA TGGCCACGTT
    GGCCTGCGTT CGGCTCTGGG TCTGGCAGAC CGGGCAGTGC AGATGCAGTG TGTGTTAAGT
    TCTGGAGGTG AATGCAGTAA GAGCTTCACT GAATGTGCCC GTGCTCTCCG GATGTTGCCT
    TGTGCCATGT CCAGCTCAGA GTCTCATGCC CTGTTAGAGG CTTGTCAGCT GGTGGTTTGG
    ACGTTGGAAG CCAGTCAGTG TAAAGGAATG GATGTGACCA CTTTGCTGGC TTGTTTTTCC
    TTTTTGGAAG AATATCAGGA ATTTCTGCTT GCACAGCAAA AGAACTCGTT TTCGCTGCAG
    GTCCAATACT CTCTTTGTTT CAGTTTTTAC CAGGGCTTCA TCTGCACATA TGATAGTCTT
    CATGCTTCTC AGGTGTCTGT GGGCGAGACC TTGGACAGAG TGCTATTGTT CTGCCGGTCG
    ACCGCAGGAC GTATGCTTGC TGAACTGCGG ACACTCAATA ATGACAACTT GCTGCTTAAA
    GCAGTGTGTG CAGTGAATAA TGTGGTGTAC GAGCTGTTTA ACAGGAAGCT GTATGAAGGA
    GCCTTTGGAT TGTCGATGAT TGTGTGTCAG GAGCTCTGTA AGGACTGCCC GCCCTCTCTT
    CCTATGGATC GGGTCAACCG CTGTTTCATG CTGACAGTGC AGTGTAGTCG CCGCGCAGGC
    CATCTGGAGC AGGCTCTGGA CTGGATTGTT CGCTGGATTC AGGCTTTGGG CTCACAGATT
    TTGGATCATC TCACTGAACC TGTGTCGATG TGGGTCAAAA CTAAGTGTGA TGCCGCCAGA
    GCAGGCGAGG ATGACACACG CCTGAGGACT CTGAGGGACG GGTTAGGAGA ATCCTTAGTG
    GATGAGGAGG TTATGATGTG TTTGTTAGAG GAGGAGTTGC GTGTATATAA GGAGCAGACT
    GGAGACACAG CACAGGAACG CTACAACACA CTCTGCGACC TCTTGGAAAT CTGCCATGAA
    GAAACCCCAC ACACACTAAG AAGAGCTACG TATCTGTGTG AGATGGCACA GGTTGTGTGC
    TATCAGGACT TCAGCCAGCA GACTGACTGC TCAGCAGTTG ATTTTACCCA CGAGGCTTTG
    CGGTTGCTGG AAGCAGAACC AGAGACGGCA GAGAATACTG ATAGGCTGAA GGATGAGAAA
    GCCCACGCTT CCCTGTGGCT CTATATCTGC ACTCTTGAGT CTAACCTGCA GGAGGCTGTG
    GACACAGAGA AGCGTTTGTG TGCAGTGCAG GAGGAGAATA AAAGAGATGT GAATATGGAT
    CCTGTTCCCA CAAATGATCT GGAGTACGAG GACAAACAGA AATCACAAGA GAGCCAGTTA
    GTCTACGATG GACTACGCTT CAACCTGCTG GCTCACAGCA AGCAAAGTGA ACCTCTAGAT
    AAATGTTTGT CTCTATGGCG GAGTCTCCTC AAGGGGGCTG TGCCTGCAGT TAGAGACCCT
    AAACTCACAG CTTCTTCCAT GACTTTGATG GCTGCCCTAT ACACACTTAT GGGCAAGCAC
    CTGCAAGCTC TGGAGGGATA TCAGCTTGCT GCCACCCTTT TCCATGTACT TGGTGATGGC
    CAGAGTAGTG CCAATGCCTT CTGCCACTCT ACCAGGATCT TGCTTTACCT GGGTTCACCG
    CAGCTTGCAC AGGTGGACTT AGAAAAAGCA GAAAAGAGCT TGTCATCTGT CACCATCTCT
    GAGGGCACAT CTATTATCTC CATGCTGGCC ATGTTGCAAA AAGCACAGCT ACACTATGCT
    CTAGGACAGG TGGAGAGTGG CGTGTCCTGC CTGATGGAGG TGATAAAGGA ATCTGCTCAG
    CATCATTCTA AGAGCTGGTA CCTGCTCAGG GCTCGAGCAC TGCAGACAGC CAGTGAATAC
    TTGAGTCTGG ACACACTGGC TTTGAACTCA CAGCTACGCC AGAGCATCAT GCAGCAGGGT
    CTAAAGACTC CAGACACTGC TCAATATGAA GGGTTGAAGC TGTTGTGTAG CCTGGTGATG
    ATGTTATTGG GCAACGGGTT TTACGGAGCG CCTGGACCAA ACACTGACAC TCGCTTTGTG
    GATAAAGGAG ACAGTGTTGT GTTTAAGTGG CTGCTGCTGA GTGAAGTGCT GGTGTGCTCG
    GAGAGAATGG TGATCACCAG GAGCAGCAGC GGAGCCGTTC ATGAAGCTAA AGCTCAGTGT
    CTAGAGGCTC TCAAACTGGC CACCAAACTA CAGACACTCA GCCACTGTGC TGAGCTGTTG
    GTGCTGAAGG CAGAGCTGGA GCTGATGAAG GGTGCAACTG AAGCTAGTAG TTTAGACCTA
    GAGCAAGTTA GACATTTACT TGATCTCTGC ACAGATTTTG GCCAGGGACG ACAACAGAAA
    TCTGAAGTGA AAATCAAACC ACGCAAAGGT CGTCCCGCTG CCCCTTCTTC CTCTGGTCAC
    ATCTCTGAGG AAGAGGATGA TCTGAGTGGG ATCCTCAGCT CTCGTGCCCT TCCGAAGGAG
    CCTGTGGAGA ACATGGCTAG ATTAGGAAGC CAAGGAGCTT CTCCCCCTCT AAAGCCCAAA
    TGCCAGCATG GCCTCTCCTG TCTGACCCAT GAAGAGACCT GCTCTTGTCT CTGCTGCACT
    GAGCTCAGCG TGGCTCGAGT CAGCATCCAC TGGGCACTAT TACAGGCAGA TCTCCAGACA
    GATCCCGAAC GCTCTCTCAG ACTACGCCAG TCTGCCAGGA AACGCTGCCG CTATGTTGTA
    ACTAAACTTC AGAGAAGTTT GGCAGCACTT GTACACTCCA AAAAATCACC TAATGTCTGC
    TTGTCACTGC TTCAGGCTGA ACAGGGTAAA GTGCATCTGG GTGCTGTGCT TCAACTGCTC
    AGAACTGGAG ACAAAGGGAA AGCTGCCATC TTGTGGGAGG AGATAGAAGC AGGATTAGAG
    GCAGTGACAC CTAAAGGAGC ACTGACACCA GAACTTGGGC CTATCAGAGC TACTCTACTG
    GGGGCTAAAG CCGTAGCCTG CTGCTTGGCG TTGGCAATGA AGAAACAGTG TATACCCGAA
    GAGCTGTTTT CTTCATTGTG GCACTGGAAT CCACCAAAGG TTAAACCGCA ATTAAAATCT
    AAGCATCGAT CCAAGAGTCC GGACGCAGCT CCTCTAGAGG CAGGTGTTAA ACATAACCCT
    AAAGTTAAGA GTGGACTTGA GGAGAATGAA AGCAGAAAAA CCAAAGAGAC CACTGTCATT
    TCGAAGAAAG CTAAAGACTC TGTGCCCAAA ATTACCATCA CAAAATCCTC CATGGTTTTT
    AAAACGCCCA AAGCAACGAG AACAACCCGG CCCAAATCTG TCTCCACTAG CACTGGCATC
    AGTGACCTGA GAGCATTTGA CTTCAATAAT GAGGTGCCTG ACATTTCAGT CAACTTCACA
    CCATCATTAC ACCGATCTAC AAACCAAAGT GCGACTGCAA AGTCAAAAGC GTCCTCAAAG
    GGTTCGTTCC AGGTTTACAA AGATTCTTCT CCTGCTGCAG AGAAAGCTGT AGTTGTCCCG
    GCTGCTCCTA AACGAACAAA GCGTTCTCGC TTCAAGGTTG AGTTCAGCGA TGAAAGCGAC
    AACGATGCTG CTCCTCTTGC TGTAATGGAA AAGTCAGAGA AAAAGAAAAA CACCTTTAAA
    ACATCACGCA CCTTGAAACC AACCCTTAAC ACCATCCCTA GTGACACCAA ACTTGTGAGC
    TCCACAGTAG ATCCAGAACC CTCCAGACGA ACACGGACCA CTAAGAAAAG CACAGCTCTT
    CCCTCCATTG GTGGCCTCTC TTCTGAGGAA GAACTTGGAG TATCTTCACA GACACGTACG
    CGTAGAGGAC GACCAAAGAA GAGCACCAGC TCAGCAGACA CAGAGGAGCC AGAGAGGATG
    AGGATGATTA AGGAGGAAGA ACAGAGGGTT CTTTTGAATG TCAGCATGGA GGAGCTCAGA
    GGCTCTGACA CTGAGACAAA AGACACTGGA AGTCCAGATG CTGACTGTGA GGTCTTGAGG
    AGAGATCTAG GTGCAGATTT TGGGCGAGAG TGTTTCAGTG AGCTCAGGAG AGCTGGACAG
    AATGGATCAG GATCTCACAC AGCTCTGCCA CATGCAACTA CATCTCCTGA CTTGTCAGTA
    GAGACGGTCC AGTCATACCT GCGCTCCTCA TGGCTCCTCC TGCACCACTT CCCGTTTCCT
    TCTCTTTATC CTCACATCTG CTCATTACTC GCTCAGTCAT TCGGTCAAAC GGACCCCATC
    ATCACAGCAA TGCTGCATGC ACAGGCACTG GGAGTGTCCA CTCGCCACCA TATGACCCGT
    CACGTGGTTA ACCAGTTCAG AAAGTTGAAG AAATCCTGTA ACGATGTAGC AGAAGGTCTT
    GGAGCTCTGA GTCTGGAGGA AACATCTGGA GCAACGCAGT CAAAGAAACT CTCTGCGTTG
    GAGCAGATCT TTTCATTCAC AGCATCAAAC CCAATGCAGT TTCCACAGAC ACACTGTCAG
    CAGTTCACAC AGCAGCTGGA AGATCTACCT GCAGGAGTGA CCGTATGCTT GCTGTCACTG
    ACGGGGGTGC ATCCTGATGA AATTGGCAAC ACTATTCTTC TGACCCGACT GGAGCGCGGC
    TCTACCCCAA TCACAGTTCG AATCCCCACT GCAGACAGAG AGCAATCTGT TGCTGGCCTG
    TTGGAAGAAA TGGATGGAGT CTTGAAAGGA CAAAAGGAAG TGAGCACAGT GGCTGAGAAA
    TCACAGTGGT GGGACGGAAG GAAGGCTTTA GATGCCCGTG TGGAGAAAAT GCTGGAGGAG
    ATGGAAGAGG CTTTAGGAGT GTGGCGAACT CTTTTTCTGC CTTTGACCTC TGATCCTAAG
    CTTGATGTTC AGGTAAAGAG CCTCCAAAAG GCTCTTAAAT CAACAAAGAT CACACGGGAC
    ACTCTCAAGG TGATTCTATC AGCGTCTCCA CTGCTGTCCC TGCCAGACCT GCAGTGTCTT
    CTGGAAGGAA TGAATGTGCA GGACAAGGAC CTGCTGAAGC TCCTGCAGAG AGGCGTGGCT
    GAACTGAGAG GGAGGGAGGA GCCACAGGGA CACACTGTTC TGATTTTAGA CAAGTACTTG
    CAGAGGTTGC CGTGGGAGAA CATTGCTTGT CTGAAGTCTC AGTCGGTCAC TCGAATGCCC
    TCATTACACG CGGTGCTTGG ACACAGTCAT CTGAGAGAGG TGGATACAAG TTGTGTTCTA
    TCTTGTGGTG TGAACCCTAA GCAGGTTTAC TATGTGCTAA ACCCAGATGG AAACCTGCCC
    GACACTGAGA ATCGCTTCAA AGAGTGGTTT ACAGGTGAAC GAACATGGCA GGGGGTTTGT
    GGAAGGGCTC CTGATCCGGA GAAACTACAA GAGGCTGTGA CCACTAAAGA TCTCTACATA
    TACATCGGAC ATGGTGCAGG AGCCCGCTTT CTGGATGCGC AGAGAGTTTT GAAGGTGCCT
    GTGAGGACCG CAGCTCTTCT GTTCGGCTGC AGTAGTGCTG CTCTCAGTGT GCTTGGTCAG
    CAGGAGGGAA CGGGCATCAT CCTCAGCTAC CTGACCGCTG GATGTCCATT GGTGCTGGGT
    AACCTGTGGG ATGTGACAGA TCGTGACTTG GATCGATTCA CATCGGCTCT GCTCCAGTCT
    TGGCTCTCTG CTGGTTCTGG CTCTTCACTT CTTCAGCATT TGGCGAAATC ACGCAGCGCA
    ACTCATTTAA AACACATAAT CGGTGCTGCG CCCATTGCGT ATGGTTTACC GGTGCATATA
    CACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021332896.1
    CDS210..6755
    Translation

    Target ORF information:

    RefSeq Version XM_021477221.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio extra spindle pole bodies like 1, separase (espl1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021477221.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    ATGGCATTAA AGAGCTTGAA AACAGAAGAT TACGTTCAGC AGTTATCCTG TGTCAAGGAC 
    ACCATAGTCC TCCACAACGA ACTGAAGAAA CATGTGGATG GTGGCTTGGG TCCTTATGGT
    CGCACAATGT GTGATCGTAT CATCAGAGCC TGCAACCAGC GGCTGGGCAA CGGCGCTGTG
    GATGCTGGCC TTCAGGAGCG GATAGTGGAG CTGGTGGAAC TGGCTGTCCA AGGGTTTCAA
    CTGCTTGAAG AGTCCGGCAT GCAGAGCACC CCCTTTTATC TGGAGAAAAT AGTCTTTCAC
    ATCCTACAGA AAGTCGCAAG CCTTGGAGCT CATGGTCCTG CTTGTCGACT GGGTCAGCTC
    GTGTATAGAA GACTGAAATG TTTGTCTGCA GAGATTGATG ACTTTAATGT CCTGGTGCGG
    AATTGCTTTG CAGTCTTGTG GAACAGTCTC CTCTCTAACA AACCGAGTGC TTCCACCCTC
    CCTCCACGAG ACAGACTCCA CTGTCAGCTG CAAGCTTTAA CATTCAGACT CCTTGAACAG
    GGCTCCAGCT CGTCCTCTTC CCCATCCAAA GTTCCTCTGT TCGTGGAGGA AGCAGTAGTG
    GAGTACGAAC GATCATGTGG AGGTTTTACT CAAGACGATG TGAGTTTTCT GACCTCTGAA
    TTGCGTGAGC TGTTCTCTGG TGCTTTCATC AATCCTCAGG ACTCTGCATC AACTTCTTCA
    CTGCTGGCTG TGTGGTGTGA GGTGGCATTT AAAGTGTCTA AACTGCTCTG CAAAAGTAGC
    TTTTCACCTG AGGCTGTTGA ACTCTTGCGA GGCACTCTGA AGGAGGTTAA TGGCCACGTT
    GGCCTGCGTT CGGCTCTGGG TCTGGCAGAC CGGGCAGTGC AGATGCAGTG TGTGTTAAGT
    TCTGGAGGTG AATGCAGTAA GAGCTTCACT GAATGTGCCC GTGCTCTCCG GATGTTGCCT
    TGTGCCATGT CCAGCTCAGA GTCTCATGCC CTGTTAGAGG CTTGTCAGCT GGTGGTTTGG
    ACGTTGGAAG CCAGTCAGTG TAAAGGAATG GATGTGACCA CTTTGCTGGC TTGTTTTTCC
    TTTTTGGAAG AATATCAGGA ATTTCTGCTT GCACAGCAAA AGAACTCGTT TTCGCTGCAG
    GTCCAATACT CTCTTTGTTT CAGTTTTTAC CAGGGCTTCA TCTGCACATA TGATAGTCTT
    CATGCTTCTC AGGTGTCTGT GGGCGAGACC TTGGACAGAG TGCTATTGTT CTGCCGGTCG
    ACCGCAGGAC GTATGCTTGC TGAACTGCGG ACACTCAATA ATGACAACTT GCTGCTTAAA
    GCAGTGTGTG CAGTGAATAA TGTGGTGTAC GAGCTGTTTA ACAGGAAGCT GTATGAAGGA
    GCCTTTGGAT TGTCGATGAT TGTGTGTCAG GAGCTCTGTA AGGACTGCCC GCCCTCTCTT
    CCTATGGATC GGGTCAACCG CTGTTTCATG CTGACAGTGC AGTGTAGTCG CCGCGCAGGC
    CATCTGGAGC AGGCTCTGGA CTGGATTGTT CGCTGGATTC AGGCTTTGGG CTCACAGATT
    TTGGATCATC TCACTGAACC TGTGTCGATG TGGGTCAAAA CTAAGTGTGA TGCCGCCAGA
    GCAGGCGAGG ATGACACACG CCTGAGGACT CTGAGGGACG GGTTAGGAGA ATCCTTAGTG
    GATGAGGAGG TTATGATGTG TTTGTTAGAG GAGGAGTTGC GTGTATATAA GGAGCAGACT
    GGAGACACAG CACAGGAACG CTACAACACA CTCTGCGACC TCTTGGAAAT CTGCCATGAA
    GAAACCCCAC ACACACTAAG AAGAGCTACG TATCTGTGTG AGATGGCACA GGTTGTGTGC
    TATCAGGACT TCAGCCAGCA GACTGACTGC TCAGCAGTTG ATTTTACCCA CGAGGCTTTG
    CGGTTGCTGG AAGCAGAACC AGAGACGGCA GAGAATACTG ATAGGCTGAA GGATGAGAAA
    GCCCACGCTT CCCTGTGGCT CTATATCTGC ACTCTTGAGT CTAACCTGCA GGAGGCTGTG
    GACACAGAGA AGCGTTTGTG TGCAGTGCAG GAGGAGAATA AAAGAGATGT GAATATGGAT
    CCTGTTCCCA CAAATGATCT GGAGTACGAG GACAAACAGA AATCACAAGA GAGCCAGTTA
    GTCTACGATG GACTACGCTT CAACCTGCTG GCTCACAGCA AGCAAAGTGA ACCTCTAGAT
    AAATGTTTGT CTCTATGGCG GAGTCTCCTC AAGGGGGCTG TGCCTGCAGT TAGAGACCCT
    AAACTCACAG CTTCTTCCAT GACTTTGATG GCTGCCCTAT ACACACTTAT GGGCAAGCAC
    CTGCAAGCTC TGGAGGGATA TCAGCTTGCT GCCACCCTTT TCCATGTACT TGGTGATGGC
    CAGAGTAGTG CCAATGCCTT CTGCCACTCT ACCAGGATCT TGCTTTACCT GGGTTCACCG
    CAGCTTGCAC AGGTGGACTT AGAAAAAGCA GAAAAGAGCT TGTCATCTGT CACCATCTCT
    GAGGGCACAT CTATTATCTC CATGCTGGCC ATGTTGCAAA AAGCACAGCT ACACTATGCT
    CTAGGACAGG TGGAGAGTGG CGTGTCCTGC CTGATGGAGG TGATAAAGGA ATCTGCTCAG
    CATCATTCTA AGAGCTGGTA CCTGCTCAGG GCTCGAGCAC TGCAGACAGC CAGTGAATAC
    TTGAGTCTGG ACACACTGGC TTTGAACTCA CAGCTACGCC AGAGCATCAT GCAGCAGGGT
    CTAAAGACTC CAGACACTGC TCAATATGAA GGGTTGAAGC TGTTGTGTAG CCTGGTGATG
    ATGTTATTGG GCAACGGGTT TTACGGAGCG CCTGGACCAA ACACTGACAC TCGCTTTGTG
    GATAAAGGAG ACAGTGTTGT GTTTAAGTGG CTGCTGCTGA GTGAAGTGCT GGTGTGCTCG
    GAGAGAATGG TGATCACCAG GAGCAGCAGC GGAGCCGTTC ATGAAGCTAA AGCTCAGTGT
    CTAGAGGCTC TCAAACTGGC CACCAAACTA CAGACACTCA GCCACTGTGC TGAGCTGTTG
    GTGCTGAAGG CAGAGCTGGA GCTGATGAAG GGTGCAACTG AAGCTAGTAG TTTAGACCTA
    GAGCAAGTTA GACATTTACT TGATCTCTGC ACAGATTTTG GCCAGGGACG ACAACAGAAA
    TCTGAAGTGA AAATCAAACC ACGCAAAGGT CGTCCCGCTG CCCCTTCTTC CTCTGGTCAC
    ATCTCTGAGG AAGAGGATGA TCTGAGTGGG ATCCTCAGCT CTCGTGCCCT TCCGAAGGAG
    CCTGTGGAGA ACATGGCTAG ATTAGGAAGC CAAGGAGCTT CTCCCCCTCT AAAGCCCAAA
    TGCCAGCATG GCCTCTCCTG TCTGACCCAT GAAGAGACCT GCTCTTGTCT CTGCTGCACT
    GAGCTCAGCG TGGCTCGAGT CAGCATCCAC TGGGCACTAT TACAGGCAGA TCTCCAGACA
    GATCCCGAAC GCTCTCTCAG ACTACGCCAG TCTGCCAGGA AACGCTGCCG CTATGTTGTA
    ACTAAACTTC AGAGAAGTTT GGCAGCACTT GTACACTCCA AAAAATCACC TAATGTCTGC
    TTGTCACTGC TTCAGGCTGA ACAGGGTAAA GTGCATCTGG GTGCTGTGCT TCAACTGCTC
    AGAACTGGAG ACAAAGGGAA AGCTGCCATC TTGTGGGAGG AGATAGAAGC AGGATTAGAG
    GCAGTGACAC CTAAAGGAGC ACTGACACCA GAACTTGGGC CTATCAGAGC TACTCTACTG
    GGGGCTAAAG CCGTAGCCTG CTGCTTGGCG TTGGCAATGA AGAAACAGTG TATACCCGAA
    GAGCTGTTTT CTTCATTGTG GCACTGGAAT CCACCAAAGG TTAAACCGCA ATTAAAATCT
    AAGCATCGAT CCAAGAGTCC GGACGCAGCT CCTCTAGAGG CAGGTGTTAA ACATAACCCT
    AAAGTTAAGA GTGGACTTGA GGAGAATGAA AGCAGAAAAA CCAAAGAGAC CACTGTCATT
    TCGAAGAAAG CTAAAGACTC TGTGCCCAAA ATTACCATCA CAAAATCCTC CATGGTTTTT
    AAAACGCCCA AAGCAACGAG AACAACCCGG CCCAAATCTG TCTCCACTAG CACTGGCATC
    AGTGACCTGA GAGCATTTGA CTTCAATAAT GAGGTGCCTG ACATTTCAGT CAACTTCACA
    CCATCATTAC ACCGATCTAC AAACCAAAGT GCGACTGCAA AGTCAAAAGC GTCCTCAAAG
    GGTTCGTTCC AGGTTTACAA AGATTCTTCT CCTGCTGCAG AGAAAGCTGT AGTTGTCCCG
    GCTGCTCCTA AACGAACAAA GCGTTCTCGC TTCAAGGTTG AGTTCAGCGA TGAAAGCGAC
    AACGATGCTG CTCCTCTTGC TGTAATGGAA AAGTCAGAGA AAAAGAAAAA CACCTTTAAA
    ACATCACGCA CCTTGAAACC AACCCTTAAC ACCATCCCTA GTGACACCAA ACTTGTGAGC
    TCCACAGTAG ATCCAGAACC CTCCAGACGA ACACGGACCA CTAAGAAAAG CACAGCTCTT
    CCCTCCATTG GTGGCCTCTC TTCTGAGGAA GAACTTGGAG TATCTTCACA GACACGTACG
    CGTAGAGGAC GACCAAAGAA GAGCACCAGC TCAGCAGACA CAGAGGAGCC AGAGAGGATG
    AGGATGATTA AGGAGGAAGA ACAGAGGGTT CTTTTGAATG TCAGCATGGA GGAGCTCAGA
    GGCTCTGACA CTGAGACAAA AGACACTGGA AGTCCAGATG CTGACTGTGA GGTCTTGAGG
    AGAGATCTAG GTGCAGATTT TGGGCGAGAG TGTTTCAGTG AGCTCAGGAG AGCTGGACAG
    AATGGATCAG GATCTCACAC AGCTCTGCCA CATGCAACTA CATCTCCTGA CTTGTCAGTA
    GAGACGGTCC AGTCATACCT GCGCTCCTCA TGGCTCCTCC TGCACCACTT CCCGTTTCCT
    TCTCTTTATC CTCACATCTG CTCATTACTC GCTCAGTCAT TCGGTCAAAC GGACCCCATC
    ATCACAGCAA TGCTGCATGC ACAGGCACTG GGAGTGTCCA CTCGCCACCA TATGACCCGT
    CACGTGGTTA ACCAGTTCAG AAAGTTGAAG AAATCCTGTA ACGATGTAGC AGAAGGTCTT
    GGAGCTCTGA GTCTGGAGGA AACATCTGGA GCAACGCAGT CAAAGAAACT CTCTGCGTTG
    GAGCAGATCT TTTCATTCAC AGCATCAAAC CCAATGCAGT TTCCACAGAC ACACTGTCAG
    CAGTTCACAC AGCAGCTGGA AGATCTACCT GCAGGAGTGA CCGTATGCTT GCTGTCACTG
    ACGGGGGTGC ATCCTGATGA AATTGGCAAC ACTATTCTTC TGACCCGACT GGAGCGCGGC
    TCTACCCCAA TCACAGTTCG AATCCCCACT GCAGACAGAG AGCAATCTGT TGCTGGCCTG
    TTGGAAGAAA TGGATGGAGT CTTGAAAGGA CAAAAGGAAG TGAGCACAGT GGCTGAGAAA
    TCACAGTGGT GGGACGGAAG GAAGGCTTTA GATGCCCGTG TGGAGAAAAT GCTGGAGGAG
    ATGGAAGAGG CTTTAGGAGT GTGGCGAACT CTTTTTCTGC CTTTGACCTC TGATCCTAAG
    CTTGATGTTC AGGTAAAGAG CCTCCAAAAG GCTCTTAAAT CAACAAAGAT CACACGGGAC
    ACTCTCAAGG TGATTCTATC AGCGTCTCCA CTGCTGTCCC TGCCAGACCT GCAGTGTCTT
    CTGGAAGGAA TGAATGTGCA GGACAAGGAC CTGCTGAAGC TCCTGCAGAG AGGCGTGGCT
    GAACTGAGAG GGAGGGAGGA GCCACAGGGA CACACTGTTC TGATTTTAGA CAAGTACTTG
    CAGAGGTTGC CGTGGGAGAA CATTGCTTGT CTGAAGTCTC AGTCGGTCAC TCGAATGCCC
    TCATTACACG CGGTGCTTGG ACACAGTCAT CTGAGAGAGG TGGATACAAG TTGTGTTCTA
    TCTTGTGGTG TGAACCCTAA GCAGGTTTAC TATGTGCTAA ACCCAGATGG AAACCTGCCC
    GACACTGAGA ATCGCTTCAA AGAGTGGTTT ACAGGTGAAC GAACATGGCA GGGGGTTTGT
    GGAAGGGCTC CTGATCCGGA GAAACTACAA GAGGCTGTGA CCACTAAAGA TCTCTACATA
    TACATCGGAC ATGGTGCAGG AGCCCGCTTT CTGGATGCGC AGAGAGTTTT GAAGGTGCCT
    GTGAGGACCG CAGCTCTTCT GTTCGGCTGC AGTAGTGCTG CTCTCAGTGT GCTTGGTCAG
    CAGGAGGGAA CGGGCATCAT CCTCAGCTAC CTGACCGCTG GATGTCCATT GGTGCTGGGT
    AACCTGTGGG ATGTGACAGA TCGTGACTTG GATCGATTCA CATCGGCTCT GCTCCAGTCT
    TGGCTCTCTG CTGGTTCTGG CTCTTCACTT CTTCAGCATT TGGCGAAATC ACGCAGCGCA
    ACTCATTTAA AACACATAAT CGGTGCTGCG CCCATTGCGT ATGGTTTACC GGTGCATATA
    CACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa12113
    Clone ID Related Accession (Same CDS sequence) XM_005166094.3 , XM_021477221.1 , XM_005166094.4
    Accession Version XM_005166094.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6546bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product separin isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395382.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005166094.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    ATGGCATTAA AGAGCTTGAA AACAGAAGAT TACGTTCAGC AGTTATCCTG TGTCAAGGAC 
    ACCATAGTCC TCCACAACGA ACTGAAGAAA CATGTGGATG GTGGCTTGGG TCCTTATGGT
    CGCACAATGT GTGATCGTAT CATCAGAGCC TGCAACCAGC GGCTGGGCAA CGGCGCTGTG
    GATGCTGGCC TTCAGGAGCG GATAGTGGAG CTGGTGGAAC TGGCTGTCCA AGGGTTTCAA
    CTGCTTGAAG AGTCCGGCAT GCAGAGCACC CCCTTTTATC TGGAGAAAAT AGTCTTTCAC
    ATCCTACAGA AAGTCGCAAG CCTTGGAGCT CATGGTCCTG CTTGTCGACT GGGTCAGCTC
    GTGTATAGAA GACTGAAATG TTTGTCTGCA GAGATTGATG ACTTTAATGT CCTGGTGCGG
    AATTGCTTTG CAGTCTTGTG GAACAGTCTC CTCTCTAACA AACCGAGTGC TTCCACCCTC
    CCTCCACGAG ACAGACTCCA CTGTCAGCTG CAAGCTTTAA CATTCAGACT CCTTGAACAG
    GGCTCCAGCT CGTCCTCTTC CCCATCCAAA GTTCCTCTGT TCGTGGAGGA AGCAGTAGTG
    GAGTACGAAC GATCATGTGG AGGTTTTACT CAAGACGATG TGAGTTTTCT GACCTCTGAA
    TTGCGTGAGC TGTTCTCTGG TGCTTTCATC AATCCTCAGG ACTCTGCATC AACTTCTTCA
    CTGCTGGCTG TGTGGTGTGA GGTGGCATTT AAAGTGTCTA AACTGCTCTG CAAAAGTAGC
    TTTTCACCTG AGGCTGTTGA ACTCTTGCGA GGCACTCTGA AGGAGGTTAA TGGCCACGTT
    GGCCTGCGTT CGGCTCTGGG TCTGGCAGAC CGGGCAGTGC AGATGCAGTG TGTGTTAAGT
    TCTGGAGGTG AATGCAGTAA GAGCTTCACT GAATGTGCCC GTGCTCTCCG GATGTTGCCT
    TGTGCCATGT CCAGCTCAGA GTCTCATGCC CTGTTAGAGG CTTGTCAGCT GGTGGTTTGG
    ACGTTGGAAG CCAGTCAGTG TAAAGGAATG GATGTGACCA CTTTGCTGGC TTGTTTTTCC
    TTTTTGGAAG AATATCAGGA ATTTCTGCTT GCACAGCAAA AGAACTCGTT TTCGCTGCAG
    GTCCAATACT CTCTTTGTTT CAGTTTTTAC CAGGGCTTCA TCTGCACATA TGATAGTCTT
    CATGCTTCTC AGGTGTCTGT GGGCGAGACC TTGGACAGAG TGCTATTGTT CTGCCGGTCG
    ACCGCAGGAC GTATGCTTGC TGAACTGCGG ACACTCAATA ATGACAACTT GCTGCTTAAA
    GCAGTGTGTG CAGTGAATAA TGTGGTGTAC GAGCTGTTTA ACAGGAAGCT GTATGAAGGA
    GCCTTTGGAT TGTCGATGAT TGTGTGTCAG GAGCTCTGTA AGGACTGCCC GCCCTCTCTT
    CCTATGGATC GGGTCAACCG CTGTTTCATG CTGACAGTGC AGTGTAGTCG CCGCGCAGGC
    CATCTGGAGC AGGCTCTGGA CTGGATTGTT CGCTGGATTC AGGCTTTGGG CTCACAGATT
    TTGGATCATC TCACTGAACC TGTGTCGATG TGGGTCAAAA CTAAGTGTGA TGCCGCCAGA
    GCAGGCGAGG ATGACACACG CCTGAGGACT CTGAGGGACG GGTTAGGAGA ATCCTTAGTG
    GATGAGGAGG TTATGATGTG TTTGTTAGAG GAGGAGTTGC GTGTATATAA GGAGCAGACT
    GGAGACACAG CACAGGAACG CTACAACACA CTCTGCGACC TCTTGGAAAT CTGCCATGAA
    GAAACCCCAC ACACACTAAG AAGAGCTACG TATCTGTGTG AGATGGCACA GGTTGTGTGC
    TATCAGGACT TCAGCCAGCA GACTGACTGC TCAGCAGTTG ATTTTACCCA CGAGGCTTTG
    CGGTTGCTGG AAGCAGAACC AGAGACGGCA GAGAATACTG ATAGGCTGAA GGATGAGAAA
    GCCCACGCTT CCCTGTGGCT CTATATCTGC ACTCTTGAGT CTAACCTGCA GGAGGCTGTG
    GACACAGAGA AGCGTTTGTG TGCAGTGCAG GAGGAGAATA AAAGAGATGT GAATATGGAT
    CCTGTTCCCA CAAATGATCT GGAGTACGAG GACAAACAGA AATCACAAGA GAGCCAGTTA
    GTCTACGATG GACTACGCTT CAACCTGCTG GCTCACAGCA AGCAAAGTGA ACCTCTAGAT
    AAATGTTTGT CTCTATGGCG GAGTCTCCTC AAGGGGGCTG TGCCTGCAGT TAGAGACCCT
    AAACTCACAG CTTCTTCCAT GACTTTGATG GCTGCCCTAT ACACACTTAT GGGCAAGCAC
    CTGCAAGCTC TGGAGGGATA TCAGCTTGCT GCCACCCTTT TCCATGTACT TGGTGATGGC
    CAGAGTAGTG CCAATGCCTT CTGCCACTCT ACCAGGATCT TGCTTTACCT GGGTTCACCG
    CAGCTTGCAC AGGTGGACTT AGAAAAAGCA GAAAAGAGCT TGTCATCTGT CACCATCTCT
    GAGGGCACAT CTATTATCTC CATGCTGGCC ATGTTGCAAA AAGCACAGCT ACACTATGCT
    CTAGGACAGG TGGAGAGTGG CGTGTCCTGC CTGATGGAGG TGATAAAGGA ATCTGCTCAG
    CATCATTCTA AGAGCTGGTA CCTGCTCAGG GCTCGAGCAC TGCAGACAGC CAGTGAATAC
    TTGAGTCTGG ACACACTGGC TTTGAACTCA CAGCTACGCC AGAGCATCAT GCAGCAGGGT
    CTAAAGACTC CAGACACTGC TCAATATGAA GGGTTGAAGC TGTTGTGTAG CCTGGTGATG
    ATGTTATTGG GCAACGGGTT TTACGGAGCG CCTGGACCAA ACACTGACAC TCGCTTTGTG
    GATAAAGGAG ACAGTGTTGT GTTTAAGTGG CTGCTGCTGA GTGAAGTGCT GGTGTGCTCG
    GAGAGAATGG TGATCACCAG GAGCAGCAGC GGAGCCGTTC ATGAAGCTAA AGCTCAGTGT
    CTAGAGGCTC TCAAACTGGC CACCAAACTA CAGACACTCA GCCACTGTGC TGAGCTGTTG
    GTGCTGAAGG CAGAGCTGGA GCTGATGAAG GGTGCAACTG AAGCTAGTAG TTTAGACCTA
    GAGCAAGTTA GACATTTACT TGATCTCTGC ACAGATTTTG GCCAGGGACG ACAACAGAAA
    TCTGAAGTGA AAATCAAACC ACGCAAAGGT CGTCCCGCTG CCCCTTCTTC CTCTGGTCAC
    ATCTCTGAGG AAGAGGATGA TCTGAGTGGG ATCCTCAGCT CTCGTGCCCT TCCGAAGGAG
    CCTGTGGAGA ACATGGCTAG ATTAGGAAGC CAAGGAGCTT CTCCCCCTCT AAAGCCCAAA
    TGCCAGCATG GCCTCTCCTG TCTGACCCAT GAAGAGACCT GCTCTTGTCT CTGCTGCACT
    GAGCTCAGCG TGGCTCGAGT CAGCATCCAC TGGGCACTAT TACAGGCAGA TCTCCAGACA
    GATCCCGAAC GCTCTCTCAG ACTACGCCAG TCTGCCAGGA AACGCTGCCG CTATGTTGTA
    ACTAAACTTC AGAGAAGTTT GGCAGCACTT GTACACTCCA AAAAATCACC TAATGTCTGC
    TTGTCACTGC TTCAGGCTGA ACAGGGTAAA GTGCATCTGG GTGCTGTGCT TCAACTGCTC
    AGAACTGGAG ACAAAGGGAA AGCTGCCATC TTGTGGGAGG AGATAGAAGC AGGATTAGAG
    GCAGTGACAC CTAAAGGAGC ACTGACACCA GAACTTGGGC CTATCAGAGC TACTCTACTG
    GGGGCTAAAG CCGTAGCCTG CTGCTTGGCG TTGGCAATGA AGAAACAGTG TATACCCGAA
    GAGCTGTTTT CTTCATTGTG GCACTGGAAT CCACCAAAGG TTAAACCGCA ATTAAAATCT
    AAGCATCGAT CCAAGAGTCC GGACGCAGCT CCTCTAGAGG CAGGTGTTAA ACATAACCCT
    AAAGTTAAGA GTGGACTTGA GGAGAATGAA AGCAGAAAAA CCAAAGAGAC CACTGTCATT
    TCGAAGAAAG CTAAAGACTC TGTGCCCAAA ATTACCATCA CAAAATCCTC CATGGTTTTT
    AAAACGCCCA AAGCAACGAG AACAACCCGG CCCAAATCTG TCTCCACTAG CACTGGCATC
    AGTGACCTGA GAGCATTTGA CTTCAATAAT GAGGTGCCTG ACATTTCAGT CAACTTCACA
    CCATCATTAC ACCGATCTAC AAACCAAAGT GCGACTGCAA AGTCAAAAGC GTCCTCAAAG
    GGTTCGTTCC AGGTTTACAA AGATTCTTCT CCTGCTGCAG AGAAAGCTGT AGTTGTCCCG
    GCTGCTCCTA AACGAACAAA GCGTTCTCGC TTCAAGGTTG AGTTCAGCGA TGAAAGCGAC
    AACGATGCTG CTCCTCTTGC TGTAATGGAA AAGTCAGAGA AAAAGAAAAA CACCTTTAAA
    ACATCACGCA CCTTGAAACC AACCCTTAAC ACCATCCCTA GTGACACCAA ACTTGTGAGC
    TCCACAGTAG ATCCAGAACC CTCCAGACGA ACACGGACCA CTAAGAAAAG CACAGCTCTT
    CCCTCCATTG GTGGCCTCTC TTCTGAGGAA GAACTTGGAG TATCTTCACA GACACGTACG
    CGTAGAGGAC GACCAAAGAA GAGCACCAGC TCAGCAGACA CAGAGGAGCC AGAGAGGATG
    AGGATGATTA AGGAGGAAGA ACAGAGGGTT CTTTTGAATG TCAGCATGGA GGAGCTCAGA
    GGCTCTGACA CTGAGACAAA AGACACTGGA AGTCCAGATG CTGACTGTGA GGTCTTGAGG
    AGAGATCTAG GTGCAGATTT TGGGCGAGAG TGTTTCAGTG AGCTCAGGAG AGCTGGACAG
    AATGGATCAG GATCTCACAC AGCTCTGCCA CATGCAACTA CATCTCCTGA CTTGTCAGTA
    GAGACGGTCC AGTCATACCT GCGCTCCTCA TGGCTCCTCC TGCACCACTT CCCGTTTCCT
    TCTCTTTATC CTCACATCTG CTCATTACTC GCTCAGTCAT TCGGTCAAAC GGACCCCATC
    ATCACAGCAA TGCTGCATGC ACAGGCACTG GGAGTGTCCA CTCGCCACCA TATGACCCGT
    CACGTGGTTA ACCAGTTCAG AAAGTTGAAG AAATCCTGTA ACGATGTAGC AGAAGGTCTT
    GGAGCTCTGA GTCTGGAGGA AACATCTGGA GCAACGCAGT CAAAGAAACT CTCTGCGTTG
    GAGCAGATCT TTTCATTCAC AGCATCAAAC CCAATGCAGT TTCCACAGAC ACACTGTCAG
    CAGTTCACAC AGCAGCTGGA AGATCTACCT GCAGGAGTGA CCGTATGCTT GCTGTCACTG
    ACGGGGGTGC ATCCTGATGA AATTGGCAAC ACTATTCTTC TGACCCGACT GGAGCGCGGC
    TCTACCCCAA TCACAGTTCG AATCCCCACT GCAGACAGAG AGCAATCTGT TGCTGGCCTG
    TTGGAAGAAA TGGATGGAGT CTTGAAAGGA CAAAAGGAAG TGAGCACAGT GGCTGAGAAA
    TCACAGTGGT GGGACGGAAG GAAGGCTTTA GATGCCCGTG TGGAGAAAAT GCTGGAGGAG
    ATGGAAGAGG CTTTAGGAGT GTGGCGAACT CTTTTTCTGC CTTTGACCTC TGATCCTAAG
    CTTGATGTTC AGGTAAAGAG CCTCCAAAAG GCTCTTAAAT CAACAAAGAT CACACGGGAC
    ACTCTCAAGG TGATTCTATC AGCGTCTCCA CTGCTGTCCC TGCCAGACCT GCAGTGTCTT
    CTGGAAGGAA TGAATGTGCA GGACAAGGAC CTGCTGAAGC TCCTGCAGAG AGGCGTGGCT
    GAACTGAGAG GGAGGGAGGA GCCACAGGGA CACACTGTTC TGATTTTAGA CAAGTACTTG
    CAGAGGTTGC CGTGGGAGAA CATTGCTTGT CTGAAGTCTC AGTCGGTCAC TCGAATGCCC
    TCATTACACG CGGTGCTTGG ACACAGTCAT CTGAGAGAGG TGGATACAAG TTGTGTTCTA
    TCTTGTGGTG TGAACCCTAA GCAGGTTTAC TATGTGCTAA ACCCAGATGG AAACCTGCCC
    GACACTGAGA ATCGCTTCAA AGAGTGGTTT ACAGGTGAAC GAACATGGCA GGGGGTTTGT
    GGAAGGGCTC CTGATCCGGA GAAACTACAA GAGGCTGTGA CCACTAAAGA TCTCTACATA
    TACATCGGAC ATGGTGCAGG AGCCCGCTTT CTGGATGCGC AGAGAGTTTT GAAGGTGCCT
    GTGAGGACCG CAGCTCTTCT GTTCGGCTGC AGTAGTGCTG CTCTCAGTGT GCTTGGTCAG
    CAGGAGGGAA CGGGCATCAT CCTCAGCTAC CTGACCGCTG GATGTCCATT GGTGCTGGGT
    AACCTGTGGG ATGTGACAGA TCGTGACTTG GATCGATTCA CATCGGCTCT GCTCCAGTCT
    TGGCTCTCTG CTGGTTCTGG CTCTTCACTT CTTCAGCATT TGGCGAAATC ACGCAGCGCA
    ACTCATTTAA AACACATAAT CGGTGCTGCG CCCATTGCGT ATGGTTTACC GGTGCATATA
    CACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005166151.1
    CDS210..6755
    Translation

    Target ORF information:

    RefSeq Version XM_005166094.4
    Organism Danio rerio(zebrafish)
    Definition Danio rerio extra spindle pole bodies like 1, separase (espl1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005166094.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    ATGGCATTAA AGAGCTTGAA AACAGAAGAT TACGTTCAGC AGTTATCCTG TGTCAAGGAC 
    ACCATAGTCC TCCACAACGA ACTGAAGAAA CATGTGGATG GTGGCTTGGG TCCTTATGGT
    CGCACAATGT GTGATCGTAT CATCAGAGCC TGCAACCAGC GGCTGGGCAA CGGCGCTGTG
    GATGCTGGCC TTCAGGAGCG GATAGTGGAG CTGGTGGAAC TGGCTGTCCA AGGGTTTCAA
    CTGCTTGAAG AGTCCGGCAT GCAGAGCACC CCCTTTTATC TGGAGAAAAT AGTCTTTCAC
    ATCCTACAGA AAGTCGCAAG CCTTGGAGCT CATGGTCCTG CTTGTCGACT GGGTCAGCTC
    GTGTATAGAA GACTGAAATG TTTGTCTGCA GAGATTGATG ACTTTAATGT CCTGGTGCGG
    AATTGCTTTG CAGTCTTGTG GAACAGTCTC CTCTCTAACA AACCGAGTGC TTCCACCCTC
    CCTCCACGAG ACAGACTCCA CTGTCAGCTG CAAGCTTTAA CATTCAGACT CCTTGAACAG
    GGCTCCAGCT CGTCCTCTTC CCCATCCAAA GTTCCTCTGT TCGTGGAGGA AGCAGTAGTG
    GAGTACGAAC GATCATGTGG AGGTTTTACT CAAGACGATG TGAGTTTTCT GACCTCTGAA
    TTGCGTGAGC TGTTCTCTGG TGCTTTCATC AATCCTCAGG ACTCTGCATC AACTTCTTCA
    CTGCTGGCTG TGTGGTGTGA GGTGGCATTT AAAGTGTCTA AACTGCTCTG CAAAAGTAGC
    TTTTCACCTG AGGCTGTTGA ACTCTTGCGA GGCACTCTGA AGGAGGTTAA TGGCCACGTT
    GGCCTGCGTT CGGCTCTGGG TCTGGCAGAC CGGGCAGTGC AGATGCAGTG TGTGTTAAGT
    TCTGGAGGTG AATGCAGTAA GAGCTTCACT GAATGTGCCC GTGCTCTCCG GATGTTGCCT
    TGTGCCATGT CCAGCTCAGA GTCTCATGCC CTGTTAGAGG CTTGTCAGCT GGTGGTTTGG
    ACGTTGGAAG CCAGTCAGTG TAAAGGAATG GATGTGACCA CTTTGCTGGC TTGTTTTTCC
    TTTTTGGAAG AATATCAGGA ATTTCTGCTT GCACAGCAAA AGAACTCGTT TTCGCTGCAG
    GTCCAATACT CTCTTTGTTT CAGTTTTTAC CAGGGCTTCA TCTGCACATA TGATAGTCTT
    CATGCTTCTC AGGTGTCTGT GGGCGAGACC TTGGACAGAG TGCTATTGTT CTGCCGGTCG
    ACCGCAGGAC GTATGCTTGC TGAACTGCGG ACACTCAATA ATGACAACTT GCTGCTTAAA
    GCAGTGTGTG CAGTGAATAA TGTGGTGTAC GAGCTGTTTA ACAGGAAGCT GTATGAAGGA
    GCCTTTGGAT TGTCGATGAT TGTGTGTCAG GAGCTCTGTA AGGACTGCCC GCCCTCTCTT
    CCTATGGATC GGGTCAACCG CTGTTTCATG CTGACAGTGC AGTGTAGTCG CCGCGCAGGC
    CATCTGGAGC AGGCTCTGGA CTGGATTGTT CGCTGGATTC AGGCTTTGGG CTCACAGATT
    TTGGATCATC TCACTGAACC TGTGTCGATG TGGGTCAAAA CTAAGTGTGA TGCCGCCAGA
    GCAGGCGAGG ATGACACACG CCTGAGGACT CTGAGGGACG GGTTAGGAGA ATCCTTAGTG
    GATGAGGAGG TTATGATGTG TTTGTTAGAG GAGGAGTTGC GTGTATATAA GGAGCAGACT
    GGAGACACAG CACAGGAACG CTACAACACA CTCTGCGACC TCTTGGAAAT CTGCCATGAA
    GAAACCCCAC ACACACTAAG AAGAGCTACG TATCTGTGTG AGATGGCACA GGTTGTGTGC
    TATCAGGACT TCAGCCAGCA GACTGACTGC TCAGCAGTTG ATTTTACCCA CGAGGCTTTG
    CGGTTGCTGG AAGCAGAACC AGAGACGGCA GAGAATACTG ATAGGCTGAA GGATGAGAAA
    GCCCACGCTT CCCTGTGGCT CTATATCTGC ACTCTTGAGT CTAACCTGCA GGAGGCTGTG
    GACACAGAGA AGCGTTTGTG TGCAGTGCAG GAGGAGAATA AAAGAGATGT GAATATGGAT
    CCTGTTCCCA CAAATGATCT GGAGTACGAG GACAAACAGA AATCACAAGA GAGCCAGTTA
    GTCTACGATG GACTACGCTT CAACCTGCTG GCTCACAGCA AGCAAAGTGA ACCTCTAGAT
    AAATGTTTGT CTCTATGGCG GAGTCTCCTC AAGGGGGCTG TGCCTGCAGT TAGAGACCCT
    AAACTCACAG CTTCTTCCAT GACTTTGATG GCTGCCCTAT ACACACTTAT GGGCAAGCAC
    CTGCAAGCTC TGGAGGGATA TCAGCTTGCT GCCACCCTTT TCCATGTACT TGGTGATGGC
    CAGAGTAGTG CCAATGCCTT CTGCCACTCT ACCAGGATCT TGCTTTACCT GGGTTCACCG
    CAGCTTGCAC AGGTGGACTT AGAAAAAGCA GAAAAGAGCT TGTCATCTGT CACCATCTCT
    GAGGGCACAT CTATTATCTC CATGCTGGCC ATGTTGCAAA AAGCACAGCT ACACTATGCT
    CTAGGACAGG TGGAGAGTGG CGTGTCCTGC CTGATGGAGG TGATAAAGGA ATCTGCTCAG
    CATCATTCTA AGAGCTGGTA CCTGCTCAGG GCTCGAGCAC TGCAGACAGC CAGTGAATAC
    TTGAGTCTGG ACACACTGGC TTTGAACTCA CAGCTACGCC AGAGCATCAT GCAGCAGGGT
    CTAAAGACTC CAGACACTGC TCAATATGAA GGGTTGAAGC TGTTGTGTAG CCTGGTGATG
    ATGTTATTGG GCAACGGGTT TTACGGAGCG CCTGGACCAA ACACTGACAC TCGCTTTGTG
    GATAAAGGAG ACAGTGTTGT GTTTAAGTGG CTGCTGCTGA GTGAAGTGCT GGTGTGCTCG
    GAGAGAATGG TGATCACCAG GAGCAGCAGC GGAGCCGTTC ATGAAGCTAA AGCTCAGTGT
    CTAGAGGCTC TCAAACTGGC CACCAAACTA CAGACACTCA GCCACTGTGC TGAGCTGTTG
    GTGCTGAAGG CAGAGCTGGA GCTGATGAAG GGTGCAACTG AAGCTAGTAG TTTAGACCTA
    GAGCAAGTTA GACATTTACT TGATCTCTGC ACAGATTTTG GCCAGGGACG ACAACAGAAA
    TCTGAAGTGA AAATCAAACC ACGCAAAGGT CGTCCCGCTG CCCCTTCTTC CTCTGGTCAC
    ATCTCTGAGG AAGAGGATGA TCTGAGTGGG ATCCTCAGCT CTCGTGCCCT TCCGAAGGAG
    CCTGTGGAGA ACATGGCTAG ATTAGGAAGC CAAGGAGCTT CTCCCCCTCT AAAGCCCAAA
    TGCCAGCATG GCCTCTCCTG TCTGACCCAT GAAGAGACCT GCTCTTGTCT CTGCTGCACT
    GAGCTCAGCG TGGCTCGAGT CAGCATCCAC TGGGCACTAT TACAGGCAGA TCTCCAGACA
    GATCCCGAAC GCTCTCTCAG ACTACGCCAG TCTGCCAGGA AACGCTGCCG CTATGTTGTA
    ACTAAACTTC AGAGAAGTTT GGCAGCACTT GTACACTCCA AAAAATCACC TAATGTCTGC
    TTGTCACTGC TTCAGGCTGA ACAGGGTAAA GTGCATCTGG GTGCTGTGCT TCAACTGCTC
    AGAACTGGAG ACAAAGGGAA AGCTGCCATC TTGTGGGAGG AGATAGAAGC AGGATTAGAG
    GCAGTGACAC CTAAAGGAGC ACTGACACCA GAACTTGGGC CTATCAGAGC TACTCTACTG
    GGGGCTAAAG CCGTAGCCTG CTGCTTGGCG TTGGCAATGA AGAAACAGTG TATACCCGAA
    GAGCTGTTTT CTTCATTGTG GCACTGGAAT CCACCAAAGG TTAAACCGCA ATTAAAATCT
    AAGCATCGAT CCAAGAGTCC GGACGCAGCT CCTCTAGAGG CAGGTGTTAA ACATAACCCT
    AAAGTTAAGA GTGGACTTGA GGAGAATGAA AGCAGAAAAA CCAAAGAGAC CACTGTCATT
    TCGAAGAAAG CTAAAGACTC TGTGCCCAAA ATTACCATCA CAAAATCCTC CATGGTTTTT
    AAAACGCCCA AAGCAACGAG AACAACCCGG CCCAAATCTG TCTCCACTAG CACTGGCATC
    AGTGACCTGA GAGCATTTGA CTTCAATAAT GAGGTGCCTG ACATTTCAGT CAACTTCACA
    CCATCATTAC ACCGATCTAC AAACCAAAGT GCGACTGCAA AGTCAAAAGC GTCCTCAAAG
    GGTTCGTTCC AGGTTTACAA AGATTCTTCT CCTGCTGCAG AGAAAGCTGT AGTTGTCCCG
    GCTGCTCCTA AACGAACAAA GCGTTCTCGC TTCAAGGTTG AGTTCAGCGA TGAAAGCGAC
    AACGATGCTG CTCCTCTTGC TGTAATGGAA AAGTCAGAGA AAAAGAAAAA CACCTTTAAA
    ACATCACGCA CCTTGAAACC AACCCTTAAC ACCATCCCTA GTGACACCAA ACTTGTGAGC
    TCCACAGTAG ATCCAGAACC CTCCAGACGA ACACGGACCA CTAAGAAAAG CACAGCTCTT
    CCCTCCATTG GTGGCCTCTC TTCTGAGGAA GAACTTGGAG TATCTTCACA GACACGTACG
    CGTAGAGGAC GACCAAAGAA GAGCACCAGC TCAGCAGACA CAGAGGAGCC AGAGAGGATG
    AGGATGATTA AGGAGGAAGA ACAGAGGGTT CTTTTGAATG TCAGCATGGA GGAGCTCAGA
    GGCTCTGACA CTGAGACAAA AGACACTGGA AGTCCAGATG CTGACTGTGA GGTCTTGAGG
    AGAGATCTAG GTGCAGATTT TGGGCGAGAG TGTTTCAGTG AGCTCAGGAG AGCTGGACAG
    AATGGATCAG GATCTCACAC AGCTCTGCCA CATGCAACTA CATCTCCTGA CTTGTCAGTA
    GAGACGGTCC AGTCATACCT GCGCTCCTCA TGGCTCCTCC TGCACCACTT CCCGTTTCCT
    TCTCTTTATC CTCACATCTG CTCATTACTC GCTCAGTCAT TCGGTCAAAC GGACCCCATC
    ATCACAGCAA TGCTGCATGC ACAGGCACTG GGAGTGTCCA CTCGCCACCA TATGACCCGT
    CACGTGGTTA ACCAGTTCAG AAAGTTGAAG AAATCCTGTA ACGATGTAGC AGAAGGTCTT
    GGAGCTCTGA GTCTGGAGGA AACATCTGGA GCAACGCAGT CAAAGAAACT CTCTGCGTTG
    GAGCAGATCT TTTCATTCAC AGCATCAAAC CCAATGCAGT TTCCACAGAC ACACTGTCAG
    CAGTTCACAC AGCAGCTGGA AGATCTACCT GCAGGAGTGA CCGTATGCTT GCTGTCACTG
    ACGGGGGTGC ATCCTGATGA AATTGGCAAC ACTATTCTTC TGACCCGACT GGAGCGCGGC
    TCTACCCCAA TCACAGTTCG AATCCCCACT GCAGACAGAG AGCAATCTGT TGCTGGCCTG
    TTGGAAGAAA TGGATGGAGT CTTGAAAGGA CAAAAGGAAG TGAGCACAGT GGCTGAGAAA
    TCACAGTGGT GGGACGGAAG GAAGGCTTTA GATGCCCGTG TGGAGAAAAT GCTGGAGGAG
    ATGGAAGAGG CTTTAGGAGT GTGGCGAACT CTTTTTCTGC CTTTGACCTC TGATCCTAAG
    CTTGATGTTC AGGTAAAGAG CCTCCAAAAG GCTCTTAAAT CAACAAAGAT CACACGGGAC
    ACTCTCAAGG TGATTCTATC AGCGTCTCCA CTGCTGTCCC TGCCAGACCT GCAGTGTCTT
    CTGGAAGGAA TGAATGTGCA GGACAAGGAC CTGCTGAAGC TCCTGCAGAG AGGCGTGGCT
    GAACTGAGAG GGAGGGAGGA GCCACAGGGA CACACTGTTC TGATTTTAGA CAAGTACTTG
    CAGAGGTTGC CGTGGGAGAA CATTGCTTGT CTGAAGTCTC AGTCGGTCAC TCGAATGCCC
    TCATTACACG CGGTGCTTGG ACACAGTCAT CTGAGAGAGG TGGATACAAG TTGTGTTCTA
    TCTTGTGGTG TGAACCCTAA GCAGGTTTAC TATGTGCTAA ACCCAGATGG AAACCTGCCC
    GACACTGAGA ATCGCTTCAA AGAGTGGTTT ACAGGTGAAC GAACATGGCA GGGGGTTTGT
    GGAAGGGCTC CTGATCCGGA GAAACTACAA GAGGCTGTGA CCACTAAAGA TCTCTACATA
    TACATCGGAC ATGGTGCAGG AGCCCGCTTT CTGGATGCGC AGAGAGTTTT GAAGGTGCCT
    GTGAGGACCG CAGCTCTTCT GTTCGGCTGC AGTAGTGCTG CTCTCAGTGT GCTTGGTCAG
    CAGGAGGGAA CGGGCATCAT CCTCAGCTAC CTGACCGCTG GATGTCCATT GGTGCTGGGT
    AACCTGTGGG ATGTGACAGA TCGTGACTTG GATCGATTCA CATCGGCTCT GCTCCAGTCT
    TGGCTCTCTG CTGGTTCTGG CTCTTCACTT CTTCAGCATT TGGCGAAATC ACGCAGCGCA
    ACTCATTTAA AACACATAAT CGGTGCTGCG CCCATTGCGT ATGGTTTACC GGTGCATATA
    CACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.