espl1 gene cDNA ORF clone, Danio rerio(zebrafish)

mRNA	Protein	Name
XM_021474123.1	XP_021329798.1	separin isoform X1
XM_005166094.3	XP_005166151.1	separin isoform X2
XM_021477220.1	XP_021332895.1	separin isoform X1
XM_021471829.1	XP_021327504.1	separin isoform X4
XM_021474124.1	XP_021329799.1	separin isoform X2
XM_001337833.4	XP_001337869.1	separin isoform X1
XM_021477221.1	XP_021332896.1	separin isoform X2
XM_001337833.5	XP_001337869.1	separin isoform X1
XM_005166094.4	XP_005166151.1	separin isoform X2
XM_017356662.1	XP_017212151.1	separin isoform X1
XM_021471828.1	XP_021327503.1	separin isoform X3

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa144755	XM_021471828.1 Latest version!	Danio rerio extra spindle pole bodies like 1, separase (espl1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
ODa144756	XM_021471829.1 Latest version!	Danio rerio extra spindle pole bodies like 1, separase (espl1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
ODa146569	XM_021474123.1 Latest version!	Danio rerio extra spindle pole bodies like 1, separase (espl1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
ODa146570	XM_021474124.1 Latest version!	Danio rerio extra spindle pole bodies like 1, separase (espl1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
ODa12112	XM_021477220.1 Latest version!	Danio rerio extra spindle pole bodies like 1, separase (espl1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
ODa12112	XM_001337833.5 Latest version!	Danio rerio extra spindle pole bodies like 1, separase (espl1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
ODa12112	XM_017356662.1 Latest version!	Danio rerio extra spindle pole bodies like 1, separase (espl1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
ODa12113	XM_005166094.4 Latest version!	Danio rerio extra spindle pole bodies like 1, separase (espl1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
ODa12113	XM_021477221.1 Latest version!	Danio rerio extra spindle pole bodies like 1, separase (espl1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
View Old Accession Versions >>
ODa12112	XM_001337833.4	Danio rerio extra spindle pole bodies like 1, separase (espl1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
ODa12113	XM_005166094.3	Danio rerio extra spindle pole bodies like 1, separase (espl1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
Hide Old Accession Versions >>

espl1 ( XM_021471828.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021327503.1 Danio rerio extra spindle pole bodies like 1, separase (espl1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa144755
Clone ID Related Accession (Same CDS sequence)	XM_021471828.1
Accession Version	XM_021471828.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6420bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	separin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394662.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTGATC GTATCATCAG AGCCTGCAAC CAGCGGCTGG GCAACGGCTC TGTGGATGCT 
GGCCTTCAGG AGCGGATAGT GGAGCTGGTG GAACTGGCTG TCCAAGGGTT TCAACTGCTT 
GAAGAGTCCG GCATGCAGAG CACCCCCTTT TATCTGGAGA AAATAGTCTT TCACATCCTA 
CAGAAAGTCG CAAGTCTTGG AGCTCATGGT CCTGCTTGTC GACTGGGTCA GCTCGTGTAT 
AGAAGACTGA AATGTTTGTC TGCAGAGATT GACGACTTTA ATGTCCTGGT GCGGAATTGC 
TTTGCAGTCT TGTGGAACAG TCTCCTCTCT AACAAACCGA GTGCTTCCAC CCTCCCTCCA 
CGAGACAGAC TCCACTGTCA GCTGCAAGCT TTAATATTCA GACTCCTTGA ACAGGGCTCC 
AGCTCGTTCT CTTCCCCATC CAAAGTTCCT CTGTTTGTTG AGGAAGCAGT AGTGGAGTAC 
GAACGATCAT GTGGAGGTTT TACTCAAGAC GATGTGAGTT TTCTGACCTC TGAATTGCGT 
GAGCTGTTCT CTGGTGCTTT ATTCAATCCT CAGGACTCTG CATCAACTTC TTCACTTCTG 
GCTGTGTGGT GTGAGGTTGC ATTTAAAGTG TCTAAACTGC TCTGCAAAAG TGGCTTTTCA 
CCTGAGGCTG TTGAACTCTT GCGAGGCACT CTGAAGGAGG TTAATGGCCA CGTTGGCCTG 
CGTTCGGCTC TGGGTCTGGC AGACCGGGCA GTGCAGATGC AGTGTGTGTT AAGTTCTGGA 
GGTGAATGCA GTAAGAGCTT CACTGAATGT GCCCGTGCTC TCCGGATGTT GCCTTGTGCC 
ATGTCCAGCT CAGAGTCTCA TGCCCTGTTA GAGGCTTGTC AGCTGGTGGT TTGGACGTTG 
GAAGCTAGTC AGTGTAAAGG AATGGATGTG ACCACTTTGC TGGCTTGTTT TTCCTTTTTG 
GAAGAATATC AGGAATTTCT CCTTACACAG CAAAAGAACT CGTTTTCGCT GCAGGTGCAA 
TACTCTCTTT GTTTCAGTTT TTACCAGGGC TTCATCTGCA CATATGATAG TCTTCATGCT 
TCTCAGGTGT CTGTGGGCGA GACCTTGGAC AGAGTGCTAT TGTTCTGCCG GTCGACCGCA 
GGACGTATGC TTGCTGAACT GCGGACACTC AATAATGACA ACTTGCTGCT TAAAGCAGTG 
TGTGCAGTGA ATAATGTGGT GTACGAGCTG TTTAACAGGA AGCTGTTTGA AGGAGCCTTT 
GGATTGTCGA TGATTGTGTG TCAGGAGCTC TGTAAGGACT GCCCGCCCTC TCTTCCTGTG 
GATCGGGTCA ACCGCTGTTT CATGCTGACA GTGCAGTGTA GTCGCCGCGC AGGCCATCTG 
GAGCAGGCTC TGGACTGGAT TGTTCGCTGG ATTCAGGCTT TGGGCTCACA GATTTTGGAT 
CATCTCACTG AACCTGTGTC GATGTGGGTC AAAACTAAGT GTGATGCCGC CAGAGCAGGC 
GAGGATGACA CACGCCTGAG GACTCTGAGG GACGGGTTAG GAGAATCCTT AGTGGATGAG 
GAGGTTATGG TGTGTTTGTT AGAGGAGGAG TTGCGTGTAT ATAAGGAGCA GACTGGAGAC 
ACAGCACAGG AACGCTACAA CACACTCTGC GACCTCTTGG AAATCTGCCA TGAAGAAACC 
CCACACACAC TAAGAAGAGC TACGTATCTG TGTGAGATGG CGCAGGTTGT GTGCTATCAG 
GACTTCAGCC AGCAGACTGA CTGCTCAGCA GTTGATTTTA CCCACGAGGC TTTGCGGTTG 
CTGGAAGCAG AACCAGAGAC GGCAGAGAAT ACTGATAGGC TGAAGGATGA GAAAGCCCAC 
GCTTCCCTGT GGCTCTATAT CTGCACTCTT GAGTCTAACC TGCAGGAGGC TGTGGACACA 
GAGAAGCGTT TGTGTGCAGT GCAGGAGGAG AATAAAACAG ATGTGAATAT GGATCCTGTT 
CCCACAAATG ATCTGGAGTA CGAGGACAAA CAGAAATCAC AAGAGAGCCA GTTAGTCTAC 
GATGGACTAC GCTTCAACCT GCTGGCTCAC AGCAAGCAAA GTGAACCTCT AGATAGATGT 
TTGTCTCTAT GGCGGAGTCT CCTCAGAGGG TCTGTGCCTG CAGTTAGAGA CCCTAAACTC 
ACAGCTTCTT CCATGACTTT GATGGCTGCT CTATACACAC TTATGGGCAA ACACCTGCAA 
GCTCTGGAGG GATATCAGCT TGCTGCCACC CTTTTCCATG TACTTGGTGA TGGTCAGAAT 
AGTGCCAATG CCTTCTGCCA CTCTACCAGG ATCTTGCTTT ACCTGGGTTC ACCGCAGCTT 
GCACAGGTGG ACTTAGAAAA AGCAGAAAAG AGCTTGTCAT CCGTCACCAT CTCTGAGGGC 
ACATCTATTA TCTCCATGCT GGCCATGTTG CAAAAAGCAC AGCTACACTA TGCTCTAGGA 
CAGGTGGAGA GTGGCGTGTC CTGCCTGATG GAGGTGATAA AGGAATCTGC TCAGCATCAT 
TCTAAGAGCT GGTACCTGCT CAGGGCTCGA GCACTGCAGA CAGCCAGTGA ATACTTGAGT 
CTGGACACAC TGGCTTTGAA CTCACAGCTA CGCCAGATCA TCATGCAGCA GGGTCTAAAG 
ACTCCAGACA CTGCTCAATA TGAAGGGTTG AAGCTGTTGT GTAGCCTGGT GATGATGTTA 
TTGGGCAACG GGTTTTACGG AGCGCCTGGA CCAAACACTG ACACTCGCTT TGTGGATAAA 
GGAGACAGTG TCGTGTTTAA GTGGTTGTTG TTGAGTGAAG TGCTGGTGTG CTCGGAGAGA 
ATGGTGATCA CCAGGAGCAG CAGCGGAGCC GTTCATGAAG CTAAAGCTCA GTGTCTAGAG 
GCTCTCAAAC TGGCCACCAA ACTGCAGACA CTCAGCCACT GTGCTGAGCT GTTGGTGCTG 
AAGGCAGAGC TGGAGCTGAT GAAGGGTGCT ACTGAAGCTA GTAGTTTAGA CCTGGAGCAA 
GTTAGACATT TACTTGATCT CTGCACAGAT TTTGGCCAGG GACGACAACA GAAATCTGAA 
GTGAAAATCA AACCACGCAA AGGTCGTCCC GCTGCCCCTT CTTCCACTGG TCACATCTTT 
GAGGAAGAGG ATGATCTGAG TGGGATCCTC AGCTCTCGTG CCCTTCCGAA GGAGCCTGTG 
GAGAACATGG CTAGATTAGG AAGCCAAGGA GCTTCTCCCC CTCTAAAGCC CAAACGCCAG 
CATGGCCTCT CCTGTCTGAC CCATGAAGAG ACCTGCTCTT GTCTCTGCTG CACTGAGCTC 
AGCGTGGCTC GAGTCAGCAT CCACTGGGCA CTATTACAGG CAGATCTCCA GACAGATCCC 
GAACGCTCTC TCAGACTACG CCAGTCTGCC AGGAAACGCT GCCGCTATGT TGTAACTAAA 
CTTCAGAGAA GTTTGGCAGC ACTTGTACAC TCCAAAAAAT CACCTAATGT CTGCTTGTCA 
CTGCTTCAGG CTGAACAGGG TAAAGTGCAT CTGGGTGCTG TGCTTCAACT GCTCAGAACT 
GGAGACAAAG GGAAAGCTGC CATCTTGTGG GAGGAGATAG AAGCAGGATT AGAGGCAGTG 
ACACCTAAAG GAGCACTGAC ACCAGAACTT GGGCCTATCA GAGCTGCTCT ACTGGGGGCT 
AAAGCCGTAG CCTGCTGCTT GGCGTTGGCC ATGAAGAAAC AGTGTATACC CGAAGAGCTG 
TTTTCTTCAT TGTGGCACTG GAATCCAACA AAGGTTAAAC CGCAATTAAA ATCTAAGCAT 
CGATCCAAGA GTCCGGACGC AGCTCCTCTA GAGCCAGGTG TTAAACATAA CCCTAAAGTT 
AAGAGTGGAC TTGAGCAGAA TGAAAGCAGA AAAAAAGAGA CCACTGTCAT TTCGAAGAAA 
GCTAAAGACT CTGTGCCCAA AATTACCATC ACAAAATCCT CCATGGTTTT TAAAACTCCC 
AAAGCAACGA GAACAACCCG GCCCAAATCG GTCTCCACTA GCACTGGCAT CAGTGACCTG 
AGAGCATTTG ACTTCAATAA TGAGGTGCCT GACATTTCAG TCAACTTCAC ACCATCATTA 
CACCGATCTA CAAACCAAAG TGCGACTGCA AAGTCAAAAG CGTCCTCAAA GGGGTCGTTC 
CAGGTTTACA AAGATTCTTC TCCTGCTGCA GAGAAAGCTG TAGTTGTCCC GGCTGCTCCT 
AAACGAACAA AGCGTTCTCG CTTCAAGGTT GAGTTCAGCG ATGAAAGCGA CAACGATGCT 
GCTCCTCTTG CTGTAATGGA AAAGTCAGAG AAAAAGAAAA ACACCTTTAA AACATCACGC 
ACCTTGAAAC CAACCCTTAA CACCATCCCT AGTGACACCA AACTTGTGAG CTCCACAGTA 
GATCCAGAAC CCTCCAGACG AACACGGACC ACTAAGAAAA GCACAGCTCT TCCCTCCATT 
GGTGGCCTCT CATCTGAGGA AGAACTTGGA GTATCTTCAC AGACACGTAC GCGTAGAGGA 
CGACCAAAGA AGAGCACCAG CTCAGCAGAC ACAGAGGAGC CAGAGAGGAT GAGGATGATT 
AAGGAGGAAG AACAGAGGGT TCTTTTGAAT GTCAGCATGG AGGAGCTCAG AGGCTCTGAC 
ACCGAGACAA AAGACACTGG AAGTCCAGAT GCTGACTGTG AGGTCTTGAG GAGAGATCTA 
GGTGCAGATT TTGGGCAAGA GTGTTTCAGT GAGCTCAGGA GAGCTGGACA GAATGGATCA 
GGATCTCACA CAGCTCTGCC ACATGCAACT ACATCTCCTG CAGACTTGTC AGTAGAGACG 
GTCCAGTCAT ACCTGCGCTC CTCATGGCTC CTCCTGCACC ACTTCCCGTT TCCTTCTCTT 
TATCCTCACA TCTGCTCATT ACTCGCTCAG TCATTCGGTC AAACGGACCC CATCATCACA 
GCAATGCTGC ATGCACAGGC ACTGGGAGTG TCCACTCGCC ACCATATGAC CCGTCACGTG 
GTTAACCAGT TCAGGAAGTT GAAGAAATCC TGTAACGATG TAGCAGAAGG TCTTGGAGCT 
CTGAGTCTGG AGGAAACATC TGGGGCAACG CAGTCAAAGA CACTCTCTGC GTTGGAGCAG 
ATCTTTTCAT TCACAGCAGC AAACCCAATG CAGTTTCCAC AGACACACTG TCAGCAGTTC 
ACACAGCAGC TGGAAGATCT ACCTGCAGGA GTGACCGTAT GCTTGCTGTC ACTGACGGGG 
GTGCATCCTG ATGAAATTGG CAACACTATT CTTCTGACCC GACTGGAGCG CGGCTCTACC 
CCAATCACAG TTCGAATCCC CACTGCAGAC AGAGAGCAAT CTGTTGCTGG CCTGTTGGAA 
GAAATGGATG GAGTCTTGAA AGGACAAAAG GAAGTGAGCA CAGTGGCTGA GAAATCACAG 
TGGTGGGACG GAAGGAAGGC TTTAGATGCC CGCGTGGAGA AAATGCTGGA GGAGATGGAA 
GAGGCTTTAG GAGTGTGGCG AACTCTTCTT CTGCCTTTGA CCTCTGATCC TAAGCTTGAT 
GTTCAGGTAA AGAGCCTCCA AAAGGCTCTT AAATCAACAA AGATCACACG GGACACTCTC 
AAGGTGATTC TTTCAGCGTC TCCACTGCTG TCCCTGCCAG ACCTGCAGTG TCTTCTGGAA 
GGAATGAATG TGCAGGACAA GGACCTGCTG AAGCTCCTGC AGAGGGCCGT GGCTGAACTG 
AGAGGGAGGG AGGAGCAACA GGGACACACT GTTCTGATTT TAGACAAGTA CTTGCAGAGG 
TTGCCGTGGG AGAACATTGC TTGTCTGAAG TCTCAGTCGG TCACTCGAAT GCCCTCATTA 
CACGCGGTGC TTGGACACAG TCATCTGAGA GAGGTGGATA CAAGTTGTGT TCTGTCTTGT 
GGTGTGAACC CTAAGCAGGT TTACTATGTG CTAAACCCAG ATGGAAACCT GCCCGACACT 
GAGAATCGCT TCAAAGAGTG GTTTACAGGT GAACGAACAT GGCAGGGGGT TTGTGGAAGG 
GCTCCTGATC CAGAGAAACT ACAAGAGGCT GTGACCACTA AGGATCTCTA CATATACATC 
GGACATGGTG CAGGAGCCCG CTTTCTGGAT GCGCAGAGAG TTTTGAAGGT GCCTGTGAGG 
GCCGCAGCTC TTCTGTTCGG CTGCAGTAGT GCTGCTCTCA GTGTGCTTGG TCAGCAGGAG 
GGAACGGGCA TCATCCTCAG CTACCTGACC GCTGGATGTC CATTGGTGCT GGGTAACCTG 
TGGGATGTGA CAGATCGTGA CTTGGATCGA TTCACATCGG CTCTGCTCCA GTCTTGGCTC 
TCTGCTGGTT CTGGCTCTTC ACTTCTTCAG CATTTGGCGA AATCACGCAG CGCAACTCAT 
TTAAAACATA TAATCGGTGC TGCGCCCATT GCGTATGGTT TACCGGTGCA TATACACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021327503.1
CDS	59..6478
Translation	MCDRIIRACNQRLGNGSVDAGLQERIVELVELAVQGFQLLEESGMQSTPFYLEKIVFHILQKVASLGAHGPACRLGQLVYRRLKCLSAEIDDFNVLVRNCFAVLWNSLLSNKPSASTLPPRDRLHCQLQALIFRLLEQGSSSFSSPSKVPLFVEEAVVEYERSCGGFTQDDVSFLTSELRELFSGALFNPQDSASTSSLLAVWCEVAFKVSKLLCKSGFSPEAVELLRGTLKEVNGHVGLRSALGLADRAVQMQCVLSSGGECSKSFTECARALRMLPCAMSSSESHALLEACQLVVWTLEASQCKGMDVTTLLACFSFLEEYQEFLLTQQKNSFSLQVQYSLCFSFYQGFICTYDSLHASQVSVGETLDRVLLFCRSTAGRMLAELRTLNNDNLLLKAVCAVNNVVYELFNRKLFEGAFGLSMIVCQELCKDCPPSLPVDRVNRCFMLTVQCSRRAGHLEQALDWIVRWIQALGSQILDHLTEPVSMWVKTKCDAARAGEDDTRLRTLRDGLGESLVDEEVMVCLLEEELRVYKEQTGDTAQERYNTLCDLLEICHEETPHTLRRATYLCEMAQVVCYQDFSQQTDCSAVDFTHEALRLLEAEPETAENTDRLKDEKAHASLWLYICTLESNLQEAVDTEKRLCAVQEENKTDVNMDPVPTNDLEYEDKQKSQESQLVYDGLRFNLLAHSKQSEPLDRCLSLWRSLLRGSVPAVRDPKLTASSMTLMAALYTLMGKHLQALEGYQLAATLFHVLGDGQNSANAFCHSTRILLYLGSPQLAQVDLEKAEKSLSSVTISEGTSIISMLAMLQKAQLHYALGQVESGVSCLMEVIKESAQHHSKSWYLLRARALQTASEYLSLDTLALNSQLRQIIMQQGLKTPDTAQYEGLKLLCSLVMMLLGNGFYGAPGPNTDTRFVDKGDSVVFKWLLLSEVLVCSERMVITRSSSGAVHEAKAQCLEALKLATKLQTLSHCAELLVLKAELELMKGATEASSLDLEQVRHLLDLCTDFGQGRQQKSEVKIKPRKGRPAAPSSTGHIFEEEDDLSGILSSRALPKEPVENMARLGSQGASPPLKPKRQHGLSCLTHEETCSCLCCTELSVARVSIHWALLQADLQTDPERSLRLRQSARKRCRYVVTKLQRSLAALVHSKKSPNVCLSLLQAEQGKVHLGAVLQLLRTGDKGKAAILWEEIEAGLEAVTPKGALTPELGPIRAALLGAKAVACCLALAMKKQCIPEELFSSLWHWNPTKVKPQLKSKHRSKSPDAAPLEPGVKHNPKVKSGLEQNESRKKETTVISKKAKDSVPKITITKSSMVFKTPKATRTTRPKSVSTSTGISDLRAFDFNNEVPDISVNFTPSLHRSTNQSATAKSKASSKGSFQVYKDSSPAAEKAVVVPAAPKRTKRSRFKVEFSDESDNDAAPLAVMEKSEKKKNTFKTSRTLKPTLNTIPSDTKLVSSTVDPEPSRRTRTTKKSTALPSIGGLSSEEELGVSSQTRTRRGRPKKSTSSADTEEPERMRMIKEEEQRVLLNVSMEELRGSDTETKDTGSPDADCEVLRRDLGADFGQECFSELRRAGQNGSGSHTALPHATTSPADLSVETVQSYLRSSWLLLHHFPFPSLYPHICSLLAQSFGQTDPIITAMLHAQALGVSTRHHMTRHVVNQFRKLKKSCNDVAEGLGALSLEETSGATQSKTLSALEQIFSFTAANPMQFPQTHCQQFTQQLEDLPAGVTVCLLSLTGVHPDEIGNTILLTRLERGSTPITVRIPTADREQSVAGLLEEMDGVLKGQKEVSTVAEKSQWWDGRKALDARVEKMLEEMEEALGVWRTLLLPLTSDPKLDVQVKSLQKALKSTKITRDTLKVILSASPLLSLPDLQCLLEGMNVQDKDLLKLLQRAVAELRGREEQQGHTVLILDKYLQRLPWENIACLKSQSVTRMPSLHAVLGHSHLREVDTSCVLSCGVNPKQVYYVLNPDGNLPDTENRFKEWFTGERTWQGVCGRAPDPEKLQEAVTTKDLYIYIGHGAGARFLDAQRVLKVPVRAAALLFGCSSAALSVLGQQEGTGIILSYLTAGCPLVLGNLWDVTDRDLDRFTSALLQSWLSAGSGSSLLQHLAKSRSATHLKHIIGAAPIAYGLPVHIH

Target ORF information:

RefSeq Version	XM_021471828.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio extra spindle pole bodies like 1, separase (espl1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021471828.1

ORF Insert Sequence:

ATGTGTGATC GTATCATCAG AGCCTGCAAC CAGCGGCTGG GCAACGGCTC TGTGGATGCT 
GGCCTTCAGG AGCGGATAGT GGAGCTGGTG GAACTGGCTG TCCAAGGGTT TCAACTGCTT 
GAAGAGTCCG GCATGCAGAG CACCCCCTTT TATCTGGAGA AAATAGTCTT TCACATCCTA 
CAGAAAGTCG CAAGTCTTGG AGCTCATGGT CCTGCTTGTC GACTGGGTCA GCTCGTGTAT 
AGAAGACTGA AATGTTTGTC TGCAGAGATT GACGACTTTA ATGTCCTGGT GCGGAATTGC 
TTTGCAGTCT TGTGGAACAG TCTCCTCTCT AACAAACCGA GTGCTTCCAC CCTCCCTCCA 
CGAGACAGAC TCCACTGTCA GCTGCAAGCT TTAATATTCA GACTCCTTGA ACAGGGCTCC 
AGCTCGTTCT CTTCCCCATC CAAAGTTCCT CTGTTTGTTG AGGAAGCAGT AGTGGAGTAC 
GAACGATCAT GTGGAGGTTT TACTCAAGAC GATGTGAGTT TTCTGACCTC TGAATTGCGT 
GAGCTGTTCT CTGGTGCTTT ATTCAATCCT CAGGACTCTG CATCAACTTC TTCACTTCTG 
GCTGTGTGGT GTGAGGTTGC ATTTAAAGTG TCTAAACTGC TCTGCAAAAG TGGCTTTTCA 
CCTGAGGCTG TTGAACTCTT GCGAGGCACT CTGAAGGAGG TTAATGGCCA CGTTGGCCTG 
CGTTCGGCTC TGGGTCTGGC AGACCGGGCA GTGCAGATGC AGTGTGTGTT AAGTTCTGGA 
GGTGAATGCA GTAAGAGCTT CACTGAATGT GCCCGTGCTC TCCGGATGTT GCCTTGTGCC 
ATGTCCAGCT CAGAGTCTCA TGCCCTGTTA GAGGCTTGTC AGCTGGTGGT TTGGACGTTG 
GAAGCTAGTC AGTGTAAAGG AATGGATGTG ACCACTTTGC TGGCTTGTTT TTCCTTTTTG 
GAAGAATATC AGGAATTTCT CCTTACACAG CAAAAGAACT CGTTTTCGCT GCAGGTGCAA 
TACTCTCTTT GTTTCAGTTT TTACCAGGGC TTCATCTGCA CATATGATAG TCTTCATGCT 
TCTCAGGTGT CTGTGGGCGA GACCTTGGAC AGAGTGCTAT TGTTCTGCCG GTCGACCGCA 
GGACGTATGC TTGCTGAACT GCGGACACTC AATAATGACA ACTTGCTGCT TAAAGCAGTG 
TGTGCAGTGA ATAATGTGGT GTACGAGCTG TTTAACAGGA AGCTGTTTGA AGGAGCCTTT 
GGATTGTCGA TGATTGTGTG TCAGGAGCTC TGTAAGGACT GCCCGCCCTC TCTTCCTGTG 
GATCGGGTCA ACCGCTGTTT CATGCTGACA GTGCAGTGTA GTCGCCGCGC AGGCCATCTG 
GAGCAGGCTC TGGACTGGAT TGTTCGCTGG ATTCAGGCTT TGGGCTCACA GATTTTGGAT 
CATCTCACTG AACCTGTGTC GATGTGGGTC AAAACTAAGT GTGATGCCGC CAGAGCAGGC 
GAGGATGACA CACGCCTGAG GACTCTGAGG GACGGGTTAG GAGAATCCTT AGTGGATGAG 
GAGGTTATGG TGTGTTTGTT AGAGGAGGAG TTGCGTGTAT ATAAGGAGCA GACTGGAGAC 
ACAGCACAGG AACGCTACAA CACACTCTGC GACCTCTTGG AAATCTGCCA TGAAGAAACC 
CCACACACAC TAAGAAGAGC TACGTATCTG TGTGAGATGG CGCAGGTTGT GTGCTATCAG 
GACTTCAGCC AGCAGACTGA CTGCTCAGCA GTTGATTTTA CCCACGAGGC TTTGCGGTTG 
CTGGAAGCAG AACCAGAGAC GGCAGAGAAT ACTGATAGGC TGAAGGATGA GAAAGCCCAC 
GCTTCCCTGT GGCTCTATAT CTGCACTCTT GAGTCTAACC TGCAGGAGGC TGTGGACACA 
GAGAAGCGTT TGTGTGCAGT GCAGGAGGAG AATAAAACAG ATGTGAATAT GGATCCTGTT 
CCCACAAATG ATCTGGAGTA CGAGGACAAA CAGAAATCAC AAGAGAGCCA GTTAGTCTAC 
GATGGACTAC GCTTCAACCT GCTGGCTCAC AGCAAGCAAA GTGAACCTCT AGATAGATGT 
TTGTCTCTAT GGCGGAGTCT CCTCAGAGGG TCTGTGCCTG CAGTTAGAGA CCCTAAACTC 
ACAGCTTCTT CCATGACTTT GATGGCTGCT CTATACACAC TTATGGGCAA ACACCTGCAA 
GCTCTGGAGG GATATCAGCT TGCTGCCACC CTTTTCCATG TACTTGGTGA TGGTCAGAAT 
AGTGCCAATG CCTTCTGCCA CTCTACCAGG ATCTTGCTTT ACCTGGGTTC ACCGCAGCTT 
GCACAGGTGG ACTTAGAAAA AGCAGAAAAG AGCTTGTCAT CCGTCACCAT CTCTGAGGGC 
ACATCTATTA TCTCCATGCT GGCCATGTTG CAAAAAGCAC AGCTACACTA TGCTCTAGGA 
CAGGTGGAGA GTGGCGTGTC CTGCCTGATG GAGGTGATAA AGGAATCTGC TCAGCATCAT 
TCTAAGAGCT GGTACCTGCT CAGGGCTCGA GCACTGCAGA CAGCCAGTGA ATACTTGAGT 
CTGGACACAC TGGCTTTGAA CTCACAGCTA CGCCAGATCA TCATGCAGCA GGGTCTAAAG 
ACTCCAGACA CTGCTCAATA TGAAGGGTTG AAGCTGTTGT GTAGCCTGGT GATGATGTTA 
TTGGGCAACG GGTTTTACGG AGCGCCTGGA CCAAACACTG ACACTCGCTT TGTGGATAAA 
GGAGACAGTG TCGTGTTTAA GTGGTTGTTG TTGAGTGAAG TGCTGGTGTG CTCGGAGAGA 
ATGGTGATCA CCAGGAGCAG CAGCGGAGCC GTTCATGAAG CTAAAGCTCA GTGTCTAGAG 
GCTCTCAAAC TGGCCACCAA ACTGCAGACA CTCAGCCACT GTGCTGAGCT GTTGGTGCTG 
AAGGCAGAGC TGGAGCTGAT GAAGGGTGCT ACTGAAGCTA GTAGTTTAGA CCTGGAGCAA 
GTTAGACATT TACTTGATCT CTGCACAGAT TTTGGCCAGG GACGACAACA GAAATCTGAA 
GTGAAAATCA AACCACGCAA AGGTCGTCCC GCTGCCCCTT CTTCCACTGG TCACATCTTT 
GAGGAAGAGG ATGATCTGAG TGGGATCCTC AGCTCTCGTG CCCTTCCGAA GGAGCCTGTG 
GAGAACATGG CTAGATTAGG AAGCCAAGGA GCTTCTCCCC CTCTAAAGCC CAAACGCCAG 
CATGGCCTCT CCTGTCTGAC CCATGAAGAG ACCTGCTCTT GTCTCTGCTG CACTGAGCTC 
AGCGTGGCTC GAGTCAGCAT CCACTGGGCA CTATTACAGG CAGATCTCCA GACAGATCCC 
GAACGCTCTC TCAGACTACG CCAGTCTGCC AGGAAACGCT GCCGCTATGT TGTAACTAAA 
CTTCAGAGAA GTTTGGCAGC ACTTGTACAC TCCAAAAAAT CACCTAATGT CTGCTTGTCA 
CTGCTTCAGG CTGAACAGGG TAAAGTGCAT CTGGGTGCTG TGCTTCAACT GCTCAGAACT 
GGAGACAAAG GGAAAGCTGC CATCTTGTGG GAGGAGATAG AAGCAGGATT AGAGGCAGTG 
ACACCTAAAG GAGCACTGAC ACCAGAACTT GGGCCTATCA GAGCTGCTCT ACTGGGGGCT 
AAAGCCGTAG CCTGCTGCTT GGCGTTGGCC ATGAAGAAAC AGTGTATACC CGAAGAGCTG 
TTTTCTTCAT TGTGGCACTG GAATCCAACA AAGGTTAAAC CGCAATTAAA ATCTAAGCAT 
CGATCCAAGA GTCCGGACGC AGCTCCTCTA GAGCCAGGTG TTAAACATAA CCCTAAAGTT 
AAGAGTGGAC TTGAGCAGAA TGAAAGCAGA AAAAAAGAGA CCACTGTCAT TTCGAAGAAA 
GCTAAAGACT CTGTGCCCAA AATTACCATC ACAAAATCCT CCATGGTTTT TAAAACTCCC 
AAAGCAACGA GAACAACCCG GCCCAAATCG GTCTCCACTA GCACTGGCAT CAGTGACCTG 
AGAGCATTTG ACTTCAATAA TGAGGTGCCT GACATTTCAG TCAACTTCAC ACCATCATTA 
CACCGATCTA CAAACCAAAG TGCGACTGCA AAGTCAAAAG CGTCCTCAAA GGGGTCGTTC 
CAGGTTTACA AAGATTCTTC TCCTGCTGCA GAGAAAGCTG TAGTTGTCCC GGCTGCTCCT 
AAACGAACAA AGCGTTCTCG CTTCAAGGTT GAGTTCAGCG ATGAAAGCGA CAACGATGCT 
GCTCCTCTTG CTGTAATGGA AAAGTCAGAG AAAAAGAAAA ACACCTTTAA AACATCACGC 
ACCTTGAAAC CAACCCTTAA CACCATCCCT AGTGACACCA AACTTGTGAG CTCCACAGTA 
GATCCAGAAC CCTCCAGACG AACACGGACC ACTAAGAAAA GCACAGCTCT TCCCTCCATT 
GGTGGCCTCT CATCTGAGGA AGAACTTGGA GTATCTTCAC AGACACGTAC GCGTAGAGGA 
CGACCAAAGA AGAGCACCAG CTCAGCAGAC ACAGAGGAGC CAGAGAGGAT GAGGATGATT 
AAGGAGGAAG AACAGAGGGT TCTTTTGAAT GTCAGCATGG AGGAGCTCAG AGGCTCTGAC 
ACCGAGACAA AAGACACTGG AAGTCCAGAT GCTGACTGTG AGGTCTTGAG GAGAGATCTA 
GGTGCAGATT TTGGGCAAGA GTGTTTCAGT GAGCTCAGGA GAGCTGGACA GAATGGATCA 
GGATCTCACA CAGCTCTGCC ACATGCAACT ACATCTCCTG CAGACTTGTC AGTAGAGACG 
GTCCAGTCAT ACCTGCGCTC CTCATGGCTC CTCCTGCACC ACTTCCCGTT TCCTTCTCTT 
TATCCTCACA TCTGCTCATT ACTCGCTCAG TCATTCGGTC AAACGGACCC CATCATCACA 
GCAATGCTGC ATGCACAGGC ACTGGGAGTG TCCACTCGCC ACCATATGAC CCGTCACGTG 
GTTAACCAGT TCAGGAAGTT GAAGAAATCC TGTAACGATG TAGCAGAAGG TCTTGGAGCT 
CTGAGTCTGG AGGAAACATC TGGGGCAACG CAGTCAAAGA CACTCTCTGC GTTGGAGCAG 
ATCTTTTCAT TCACAGCAGC AAACCCAATG CAGTTTCCAC AGACACACTG TCAGCAGTTC 
ACACAGCAGC TGGAAGATCT ACCTGCAGGA GTGACCGTAT GCTTGCTGTC ACTGACGGGG 
GTGCATCCTG ATGAAATTGG CAACACTATT CTTCTGACCC GACTGGAGCG CGGCTCTACC 
CCAATCACAG TTCGAATCCC CACTGCAGAC AGAGAGCAAT CTGTTGCTGG CCTGTTGGAA 
GAAATGGATG GAGTCTTGAA AGGACAAAAG GAAGTGAGCA CAGTGGCTGA GAAATCACAG 
TGGTGGGACG GAAGGAAGGC TTTAGATGCC CGCGTGGAGA AAATGCTGGA GGAGATGGAA 
GAGGCTTTAG GAGTGTGGCG AACTCTTCTT CTGCCTTTGA CCTCTGATCC TAAGCTTGAT 
GTTCAGGTAA AGAGCCTCCA AAAGGCTCTT AAATCAACAA AGATCACACG GGACACTCTC 
AAGGTGATTC TTTCAGCGTC TCCACTGCTG TCCCTGCCAG ACCTGCAGTG TCTTCTGGAA 
GGAATGAATG TGCAGGACAA GGACCTGCTG AAGCTCCTGC AGAGGGCCGT GGCTGAACTG 
AGAGGGAGGG AGGAGCAACA GGGACACACT GTTCTGATTT TAGACAAGTA CTTGCAGAGG 
TTGCCGTGGG AGAACATTGC TTGTCTGAAG TCTCAGTCGG TCACTCGAAT GCCCTCATTA 
CACGCGGTGC TTGGACACAG TCATCTGAGA GAGGTGGATA CAAGTTGTGT TCTGTCTTGT 
GGTGTGAACC CTAAGCAGGT TTACTATGTG CTAAACCCAG ATGGAAACCT GCCCGACACT 
GAGAATCGCT TCAAAGAGTG GTTTACAGGT GAACGAACAT GGCAGGGGGT TTGTGGAAGG 
GCTCCTGATC CAGAGAAACT ACAAGAGGCT GTGACCACTA AGGATCTCTA CATATACATC 
GGACATGGTG CAGGAGCCCG CTTTCTGGAT GCGCAGAGAG TTTTGAAGGT GCCTGTGAGG 
GCCGCAGCTC TTCTGTTCGG CTGCAGTAGT GCTGCTCTCA GTGTGCTTGG TCAGCAGGAG 
GGAACGGGCA TCATCCTCAG CTACCTGACC GCTGGATGTC CATTGGTGCT GGGTAACCTG 
TGGGATGTGA CAGATCGTGA CTTGGATCGA TTCACATCGG CTCTGCTCCA GTCTTGGCTC 
TCTGCTGGTT CTGGCTCTTC ACTTCTTCAG CATTTGGCGA AATCACGCAG CGCAACTCAT 
TTAAAACATA TAATCGGTGC TGCGCCCATT GCGTATGGTT TACCGGTGCA TATACACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

espl1 ( XM_021471829.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021327504.1 Danio rerio extra spindle pole bodies like 1, separase (espl1), transcript variant X4, mRNA.

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CloneID	ODa144756
Clone ID Related Accession (Same CDS sequence)	XM_021471829.1
Accession Version	XM_021471829.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6417bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	separin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394662.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTGATC GTATCATCAG AGCCTGCAAC CAGCGGCTGG GCAACGGCTC TGTGGATGCT 
GGCCTTCAGG AGCGGATAGT GGAGCTGGTG GAACTGGCTG TCCAAGGGTT TCAACTGCTT 
GAAGAGTCCG GCATGCAGAG CACCCCCTTT TATCTGGAGA AAATAGTCTT TCACATCCTA 
CAGAAAGTCG CAAGTCTTGG AGCTCATGGT CCTGCTTGTC GACTGGGTCA GCTCGTGTAT 
AGAAGACTGA AATGTTTGTC TGCAGAGATT GACGACTTTA ATGTCCTGGT GCGGAATTGC 
TTTGCAGTCT TGTGGAACAG TCTCCTCTCT AACAAACCGA GTGCTTCCAC CCTCCCTCCA 
CGAGACAGAC TCCACTGTCA GCTGCAAGCT TTAATATTCA GACTCCTTGA ACAGGGCTCC 
AGCTCGTTCT CTTCCCCATC CAAAGTTCCT CTGTTTGTTG AGGAAGCAGT AGTGGAGTAC 
GAACGATCAT GTGGAGGTTT TACTCAAGAC GATGTGAGTT TTCTGACCTC TGAATTGCGT 
GAGCTGTTCT CTGGTGCTTT ATTCAATCCT CAGGACTCTG CATCAACTTC TTCACTTCTG 
GCTGTGTGGT GTGAGGTTGC ATTTAAAGTG TCTAAACTGC TCTGCAAAAG TGGCTTTTCA 
CCTGAGGCTG TTGAACTCTT GCGAGGCACT CTGAAGGAGG TTAATGGCCA CGTTGGCCTG 
CGTTCGGCTC TGGGTCTGGC AGACCGGGCA GTGCAGATGC AGTGTGTGTT AAGTTCTGGA 
GGTGAATGCA GTAAGAGCTT CACTGAATGT GCCCGTGCTC TCCGGATGTT GCCTTGTGCC 
ATGTCCAGCT CAGAGTCTCA TGCCCTGTTA GAGGCTTGTC AGCTGGTGGT TTGGACGTTG 
GAAGCTAGTC AGTGTAAAGG AATGGATGTG ACCACTTTGC TGGCTTGTTT TTCCTTTTTG 
GAAGAATATC AGGAATTTCT CCTTACACAG CAAAAGAACT CGTTTTCGCT GCAGGTGCAA 
TACTCTCTTT GTTTCAGTTT TTACCAGGGC TTCATCTGCA CATATGATAG TCTTCATGCT 
TCTCAGGTGT CTGTGGGCGA GACCTTGGAC AGAGTGCTAT TGTTCTGCCG GTCGACCGCA 
GGACGTATGC TTGCTGAACT GCGGACACTC AATAATGACA ACTTGCTGCT TAAAGCAGTG 
TGTGCAGTGA ATAATGTGGT GTACGAGCTG TTTAACAGGA AGCTGTTTGA AGGAGCCTTT 
GGATTGTCGA TGATTGTGTG TCAGGAGCTC TGTAAGGACT GCCCGCCCTC TCTTCCTGTG 
GATCGGGTCA ACCGCTGTTT CATGCTGACA GTGCAGTGTA GTCGCCGCGC AGGCCATCTG 
GAGCAGGCTC TGGACTGGAT TGTTCGCTGG ATTCAGGCTT TGGGCTCACA GATTTTGGAT 
CATCTCACTG AACCTGTGTC GATGTGGGTC AAAACTAAGT GTGATGCCGC CAGAGCAGGC 
GAGGATGACA CACGCCTGAG GACTCTGAGG GACGGGTTAG GAGAATCCTT AGTGGATGAG 
GAGGTTATGG TGTGTTTGTT AGAGGAGGAG TTGCGTGTAT ATAAGGAGCA GACTGGAGAC 
ACAGCACAGG AACGCTACAA CACACTCTGC GACCTCTTGG AAATCTGCCA TGAAGAAACC 
CCACACACAC TAAGAAGAGC TACGTATCTG TGTGAGATGG CGCAGGTTGT GTGCTATCAG 
GACTTCAGCC AGCAGACTGA CTGCTCAGCA GTTGATTTTA CCCACGAGGC TTTGCGGTTG 
CTGGAAGCAG AACCAGAGAC GGCAGAGAAT ACTGATAGGC TGAAGGATGA GAAAGCCCAC 
GCTTCCCTGT GGCTCTATAT CTGCACTCTT GAGTCTAACC TGCAGGAGGC TGTGGACACA 
GAGAAGCGTT TGTGTGCAGT GCAGGAGGAG AATAAAACAG ATGTGAATAT GGATCCTGTT 
CCCACAAATG ATCTGGAGTA CGAGGACAAA CAGAAATCAC AAGAGAGCCA GTTAGTCTAC 
GATGGACTAC GCTTCAACCT GCTGGCTCAC AGCAAGCAAA GTGAACCTCT AGATAGATGT 
TTGTCTCTAT GGCGGAGTCT CCTCAGAGGG TCTGTGCCTG CAGTTAGAGA CCCTAAACTC 
ACAGCTTCTT CCATGACTTT GATGGCTGCT CTATACACAC TTATGGGCAA ACACCTGCAA 
GCTCTGGAGG GATATCAGCT TGCTGCCACC CTTTTCCATG TACTTGGTGA TGGTCAGAAT 
AGTGCCAATG CCTTCTGCCA CTCTACCAGG ATCTTGCTTT ACCTGGGTTC ACCGCAGCTT 
GCACAGGTGG ACTTAGAAAA AGCAGAAAAG AGCTTGTCAT CCGTCACCAT CTCTGAGGGC 
ACATCTATTA TCTCCATGCT GGCCATGTTG CAAAAAGCAC AGCTACACTA TGCTCTAGGA 
CAGGTGGAGA GTGGCGTGTC CTGCCTGATG GAGGTGATAA AGGAATCTGC TCAGCATCAT 
TCTAAGAGCT GGTACCTGCT CAGGGCTCGA GCACTGCAGA CAGCCAGTGA ATACTTGAGT 
CTGGACACAC TGGCTTTGAA CTCACAGCTA CGCCAGATCA TCATGCAGCA GGGTCTAAAG 
ACTCCAGACA CTGCTCAATA TGAAGGGTTG AAGCTGTTGT GTAGCCTGGT GATGATGTTA 
TTGGGCAACG GGTTTTACGG AGCGCCTGGA CCAAACACTG ACACTCGCTT TGTGGATAAA 
GGAGACAGTG TCGTGTTTAA GTGGTTGTTG TTGAGTGAAG TGCTGGTGTG CTCGGAGAGA 
ATGGTGATCA CCAGGAGCAG CAGCGGAGCC GTTCATGAAG CTAAAGCTCA GTGTCTAGAG 
GCTCTCAAAC TGGCCACCAA ACTGCAGACA CTCAGCCACT GTGCTGAGCT GTTGGTGCTG 
AAGGCAGAGC TGGAGCTGAT GAAGGGTGCT ACTGAAGCTA GTAGTTTAGA CCTGGAGCAA 
GTTAGACATT TACTTGATCT CTGCACAGAT TTTGGCCAGG GACGACAACA GAAATCTGAA 
GTGAAAATCA AACCACGCAA AGGTCGTCCC GCTGCCCCTT CTTCCACTGG TCACATCTTT 
GAGGAAGAGG ATGATCTGAG TGGGATCCTC AGCTCTCGTG CCCTTCCGAA GGAGCCTGTG 
GAGAACATGG CTAGATTAGG AAGCCAAGGA GCTTCTCCCC CTCTAAAGCC CAAACGCCAG 
CATGGCCTCT CCTGTCTGAC CCATGAAGAG ACCTGCTCTT GTCTCTGCTG CACTGAGCTC 
AGCGTGGCTC GAGTCAGCAT CCACTGGGCA CTATTACAGG CAGATCTCCA GACAGATCCC 
GAACGCTCTC TCAGACTACG CCAGTCTGCC AGGAAACGCT GCCGCTATGT TGTAACTAAA 
CTTCAGAGAA GTTTGGCAGC ACTTGTACAC TCCAAAAAAT CACCTAATGT CTGCTTGTCA 
CTGCTTCAGG CTGAACAGGG TAAAGTGCAT CTGGGTGCTG TGCTTCAACT GCTCAGAACT 
GGAGACAAAG GGAAAGCTGC CATCTTGTGG GAGGAGATAG AAGCAGGATT AGAGGCAGTG 
ACACCTAAAG GAGCACTGAC ACCAGAACTT GGGCCTATCA GAGCTGCTCT ACTGGGGGCT 
AAAGCCGTAG CCTGCTGCTT GGCGTTGGCC ATGAAGAAAC AGTGTATACC CGAAGAGCTG 
TTTTCTTCAT TGTGGCACTG GAATCCAACA AAGGTTAAAC CGCAATTAAA ATCTAAGCAT 
CGATCCAAGA GTCCGGACGC AGCTCCTCTA GAGCCAGGTG TTAAACATAA CCCTAAAGTT 
AAGAGTGGAC TTGAGCAGAA TGAAAGCAGA AAAAAAGAGA CCACTGTCAT TTCGAAGAAA 
GCTAAAGACT CTGTGCCCAA AATTACCATC ACAAAATCCT CCATGGTTTT TAAAACTCCC 
AAAGCAACGA GAACAACCCG GCCCAAATCG GTCTCCACTA GCACTGGCAT CAGTGACCTG 
AGAGCATTTG ACTTCAATAA TGAGGTGCCT GACATTTCAG TCAACTTCAC ACCATCATTA 
CACCGATCTA CAAACCAAAG TGCGACTGCA AAGTCAAAAG CGTCCTCAAA GGGGTCGTTC 
CAGGTTTACA AAGATTCTTC TCCTGCTGCA GAGAAAGCTG TAGTTGTCCC GGCTGCTCCT 
AAACGAACAA AGCGTTCTCG CTTCAAGGTT GAGTTCAGCG ATGAAAGCGA CAACGATGCT 
GCTCCTCTTG CTGTAATGGA AAAGTCAGAG AAAAAGAAAA ACACCTTTAA AACATCACGC 
ACCTTGAAAC CAACCCTTAA CACCATCCCT AGTGACACCA AACTTGTGAG CTCCACAGTA 
GATCCAGAAC CCTCCAGACG AACACGGACC ACTAAGAAAA GCACAGCTCT TCCCTCCATT 
GGTGGCCTCT CATCTGAGGA AGAACTTGGA GTATCTTCAC AGACACGTAC GCGTAGAGGA 
CGACCAAAGA AGAGCACCAG CTCAGCAGAC ACAGAGGAGC CAGAGAGGAT GAGGATGATT 
AAGGAGGAAG AACAGAGGGT TCTTTTGAAT GTCAGCATGG AGGAGCTCAG AGGCTCTGAC 
ACCGAGACAA AAGACACTGG AAGTCCAGAT GCTGACTGTG AGGTCTTGAG GAGAGATCTA 
GGTGCAGATT TTGGGCAAGA GTGTTTCAGT GAGCTCAGGA GAGCTGGACA GAATGGATCA 
GGATCTCACA CAGCTCTGCC ACATGCAACT ACATCTCCTG ACTTGTCAGT AGAGACGGTC 
CAGTCATACC TGCGCTCCTC ATGGCTCCTC CTGCACCACT TCCCGTTTCC TTCTCTTTAT 
CCTCACATCT GCTCATTACT CGCTCAGTCA TTCGGTCAAA CGGACCCCAT CATCACAGCA 
ATGCTGCATG CACAGGCACT GGGAGTGTCC ACTCGCCACC ATATGACCCG TCACGTGGTT 
AACCAGTTCA GGAAGTTGAA GAAATCCTGT AACGATGTAG CAGAAGGTCT TGGAGCTCTG 
AGTCTGGAGG AAACATCTGG GGCAACGCAG TCAAAGACAC TCTCTGCGTT GGAGCAGATC 
TTTTCATTCA CAGCAGCAAA CCCAATGCAG TTTCCACAGA CACACTGTCA GCAGTTCACA 
CAGCAGCTGG AAGATCTACC TGCAGGAGTG ACCGTATGCT TGCTGTCACT GACGGGGGTG 
CATCCTGATG AAATTGGCAA CACTATTCTT CTGACCCGAC TGGAGCGCGG CTCTACCCCA 
ATCACAGTTC GAATCCCCAC TGCAGACAGA GAGCAATCTG TTGCTGGCCT GTTGGAAGAA 
ATGGATGGAG TCTTGAAAGG ACAAAAGGAA GTGAGCACAG TGGCTGAGAA ATCACAGTGG 
TGGGACGGAA GGAAGGCTTT AGATGCCCGC GTGGAGAAAA TGCTGGAGGA GATGGAAGAG 
GCTTTAGGAG TGTGGCGAAC TCTTCTTCTG CCTTTGACCT CTGATCCTAA GCTTGATGTT 
CAGGTAAAGA GCCTCCAAAA GGCTCTTAAA TCAACAAAGA TCACACGGGA CACTCTCAAG 
GTGATTCTTT CAGCGTCTCC ACTGCTGTCC CTGCCAGACC TGCAGTGTCT TCTGGAAGGA 
ATGAATGTGC AGGACAAGGA CCTGCTGAAG CTCCTGCAGA GGGCCGTGGC TGAACTGAGA 
GGGAGGGAGG AGCAACAGGG ACACACTGTT CTGATTTTAG ACAAGTACTT GCAGAGGTTG 
CCGTGGGAGA ACATTGCTTG TCTGAAGTCT CAGTCGGTCA CTCGAATGCC CTCATTACAC 
GCGGTGCTTG GACACAGTCA TCTGAGAGAG GTGGATACAA GTTGTGTTCT GTCTTGTGGT 
GTGAACCCTA AGCAGGTTTA CTATGTGCTA AACCCAGATG GAAACCTGCC CGACACTGAG 
AATCGCTTCA AAGAGTGGTT TACAGGTGAA CGAACATGGC AGGGGGTTTG TGGAAGGGCT 
CCTGATCCAG AGAAACTACA AGAGGCTGTG ACCACTAAGG ATCTCTACAT ATACATCGGA 
CATGGTGCAG GAGCCCGCTT TCTGGATGCG CAGAGAGTTT TGAAGGTGCC TGTGAGGGCC 
GCAGCTCTTC TGTTCGGCTG CAGTAGTGCT GCTCTCAGTG TGCTTGGTCA GCAGGAGGGA 
ACGGGCATCA TCCTCAGCTA CCTGACCGCT GGATGTCCAT TGGTGCTGGG TAACCTGTGG 
GATGTGACAG ATCGTGACTT GGATCGATTC ACATCGGCTC TGCTCCAGTC TTGGCTCTCT 
GCTGGTTCTG GCTCTTCACT TCTTCAGCAT TTGGCGAAAT CACGCAGCGC AACTCATTTA 
AAACATATAA TCGGTGCTGC GCCCATTGCG TATGGTTTAC CGGTGCATAT ACACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021327504.1
CDS	59..6475
Translation	MCDRIIRACNQRLGNGSVDAGLQERIVELVELAVQGFQLLEESGMQSTPFYLEKIVFHILQKVASLGAHGPACRLGQLVYRRLKCLSAEIDDFNVLVRNCFAVLWNSLLSNKPSASTLPPRDRLHCQLQALIFRLLEQGSSSFSSPSKVPLFVEEAVVEYERSCGGFTQDDVSFLTSELRELFSGALFNPQDSASTSSLLAVWCEVAFKVSKLLCKSGFSPEAVELLRGTLKEVNGHVGLRSALGLADRAVQMQCVLSSGGECSKSFTECARALRMLPCAMSSSESHALLEACQLVVWTLEASQCKGMDVTTLLACFSFLEEYQEFLLTQQKNSFSLQVQYSLCFSFYQGFICTYDSLHASQVSVGETLDRVLLFCRSTAGRMLAELRTLNNDNLLLKAVCAVNNVVYELFNRKLFEGAFGLSMIVCQELCKDCPPSLPVDRVNRCFMLTVQCSRRAGHLEQALDWIVRWIQALGSQILDHLTEPVSMWVKTKCDAARAGEDDTRLRTLRDGLGESLVDEEVMVCLLEEELRVYKEQTGDTAQERYNTLCDLLEICHEETPHTLRRATYLCEMAQVVCYQDFSQQTDCSAVDFTHEALRLLEAEPETAENTDRLKDEKAHASLWLYICTLESNLQEAVDTEKRLCAVQEENKTDVNMDPVPTNDLEYEDKQKSQESQLVYDGLRFNLLAHSKQSEPLDRCLSLWRSLLRGSVPAVRDPKLTASSMTLMAALYTLMGKHLQALEGYQLAATLFHVLGDGQNSANAFCHSTRILLYLGSPQLAQVDLEKAEKSLSSVTISEGTSIISMLAMLQKAQLHYALGQVESGVSCLMEVIKESAQHHSKSWYLLRARALQTASEYLSLDTLALNSQLRQIIMQQGLKTPDTAQYEGLKLLCSLVMMLLGNGFYGAPGPNTDTRFVDKGDSVVFKWLLLSEVLVCSERMVITRSSSGAVHEAKAQCLEALKLATKLQTLSHCAELLVLKAELELMKGATEASSLDLEQVRHLLDLCTDFGQGRQQKSEVKIKPRKGRPAAPSSTGHIFEEEDDLSGILSSRALPKEPVENMARLGSQGASPPLKPKRQHGLSCLTHEETCSCLCCTELSVARVSIHWALLQADLQTDPERSLRLRQSARKRCRYVVTKLQRSLAALVHSKKSPNVCLSLLQAEQGKVHLGAVLQLLRTGDKGKAAILWEEIEAGLEAVTPKGALTPELGPIRAALLGAKAVACCLALAMKKQCIPEELFSSLWHWNPTKVKPQLKSKHRSKSPDAAPLEPGVKHNPKVKSGLEQNESRKKETTVISKKAKDSVPKITITKSSMVFKTPKATRTTRPKSVSTSTGISDLRAFDFNNEVPDISVNFTPSLHRSTNQSATAKSKASSKGSFQVYKDSSPAAEKAVVVPAAPKRTKRSRFKVEFSDESDNDAAPLAVMEKSEKKKNTFKTSRTLKPTLNTIPSDTKLVSSTVDPEPSRRTRTTKKSTALPSIGGLSSEEELGVSSQTRTRRGRPKKSTSSADTEEPERMRMIKEEEQRVLLNVSMEELRGSDTETKDTGSPDADCEVLRRDLGADFGQECFSELRRAGQNGSGSHTALPHATTSPDLSVETVQSYLRSSWLLLHHFPFPSLYPHICSLLAQSFGQTDPIITAMLHAQALGVSTRHHMTRHVVNQFRKLKKSCNDVAEGLGALSLEETSGATQSKTLSALEQIFSFTAANPMQFPQTHCQQFTQQLEDLPAGVTVCLLSLTGVHPDEIGNTILLTRLERGSTPITVRIPTADREQSVAGLLEEMDGVLKGQKEVSTVAEKSQWWDGRKALDARVEKMLEEMEEALGVWRTLLLPLTSDPKLDVQVKSLQKALKSTKITRDTLKVILSASPLLSLPDLQCLLEGMNVQDKDLLKLLQRAVAELRGREEQQGHTVLILDKYLQRLPWENIACLKSQSVTRMPSLHAVLGHSHLREVDTSCVLSCGVNPKQVYYVLNPDGNLPDTENRFKEWFTGERTWQGVCGRAPDPEKLQEAVTTKDLYIYIGHGAGARFLDAQRVLKVPVRAAALLFGCSSAALSVLGQQEGTGIILSYLTAGCPLVLGNLWDVTDRDLDRFTSALLQSWLSAGSGSSLLQHLAKSRSATHLKHIIGAAPIAYGLPVHIH

Target ORF information:

RefSeq Version	XM_021471829.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio extra spindle pole bodies like 1, separase (espl1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021471829.1

ORF Insert Sequence:

ATGTGTGATC GTATCATCAG AGCCTGCAAC CAGCGGCTGG GCAACGGCTC TGTGGATGCT 
GGCCTTCAGG AGCGGATAGT GGAGCTGGTG GAACTGGCTG TCCAAGGGTT TCAACTGCTT 
GAAGAGTCCG GCATGCAGAG CACCCCCTTT TATCTGGAGA AAATAGTCTT TCACATCCTA 
CAGAAAGTCG CAAGTCTTGG AGCTCATGGT CCTGCTTGTC GACTGGGTCA GCTCGTGTAT 
AGAAGACTGA AATGTTTGTC TGCAGAGATT GACGACTTTA ATGTCCTGGT GCGGAATTGC 
TTTGCAGTCT TGTGGAACAG TCTCCTCTCT AACAAACCGA GTGCTTCCAC CCTCCCTCCA 
CGAGACAGAC TCCACTGTCA GCTGCAAGCT TTAATATTCA GACTCCTTGA ACAGGGCTCC 
AGCTCGTTCT CTTCCCCATC CAAAGTTCCT CTGTTTGTTG AGGAAGCAGT AGTGGAGTAC 
GAACGATCAT GTGGAGGTTT TACTCAAGAC GATGTGAGTT TTCTGACCTC TGAATTGCGT 
GAGCTGTTCT CTGGTGCTTT ATTCAATCCT CAGGACTCTG CATCAACTTC TTCACTTCTG 
GCTGTGTGGT GTGAGGTTGC ATTTAAAGTG TCTAAACTGC TCTGCAAAAG TGGCTTTTCA 
CCTGAGGCTG TTGAACTCTT GCGAGGCACT CTGAAGGAGG TTAATGGCCA CGTTGGCCTG 
CGTTCGGCTC TGGGTCTGGC AGACCGGGCA GTGCAGATGC AGTGTGTGTT AAGTTCTGGA 
GGTGAATGCA GTAAGAGCTT CACTGAATGT GCCCGTGCTC TCCGGATGTT GCCTTGTGCC 
ATGTCCAGCT CAGAGTCTCA TGCCCTGTTA GAGGCTTGTC AGCTGGTGGT TTGGACGTTG 
GAAGCTAGTC AGTGTAAAGG AATGGATGTG ACCACTTTGC TGGCTTGTTT TTCCTTTTTG 
GAAGAATATC AGGAATTTCT CCTTACACAG CAAAAGAACT CGTTTTCGCT GCAGGTGCAA 
TACTCTCTTT GTTTCAGTTT TTACCAGGGC TTCATCTGCA CATATGATAG TCTTCATGCT 
TCTCAGGTGT CTGTGGGCGA GACCTTGGAC AGAGTGCTAT TGTTCTGCCG GTCGACCGCA 
GGACGTATGC TTGCTGAACT GCGGACACTC AATAATGACA ACTTGCTGCT TAAAGCAGTG 
TGTGCAGTGA ATAATGTGGT GTACGAGCTG TTTAACAGGA AGCTGTTTGA AGGAGCCTTT 
GGATTGTCGA TGATTGTGTG TCAGGAGCTC TGTAAGGACT GCCCGCCCTC TCTTCCTGTG 
GATCGGGTCA ACCGCTGTTT CATGCTGACA GTGCAGTGTA GTCGCCGCGC AGGCCATCTG 
GAGCAGGCTC TGGACTGGAT TGTTCGCTGG ATTCAGGCTT TGGGCTCACA GATTTTGGAT 
CATCTCACTG AACCTGTGTC GATGTGGGTC AAAACTAAGT GTGATGCCGC CAGAGCAGGC 
GAGGATGACA CACGCCTGAG GACTCTGAGG GACGGGTTAG GAGAATCCTT AGTGGATGAG 
GAGGTTATGG TGTGTTTGTT AGAGGAGGAG TTGCGTGTAT ATAAGGAGCA GACTGGAGAC 
ACAGCACAGG AACGCTACAA CACACTCTGC GACCTCTTGG AAATCTGCCA TGAAGAAACC 
CCACACACAC TAAGAAGAGC TACGTATCTG TGTGAGATGG CGCAGGTTGT GTGCTATCAG 
GACTTCAGCC AGCAGACTGA CTGCTCAGCA GTTGATTTTA CCCACGAGGC TTTGCGGTTG 
CTGGAAGCAG AACCAGAGAC GGCAGAGAAT ACTGATAGGC TGAAGGATGA GAAAGCCCAC 
GCTTCCCTGT GGCTCTATAT CTGCACTCTT GAGTCTAACC TGCAGGAGGC TGTGGACACA 
GAGAAGCGTT TGTGTGCAGT GCAGGAGGAG AATAAAACAG ATGTGAATAT GGATCCTGTT 
CCCACAAATG ATCTGGAGTA CGAGGACAAA CAGAAATCAC AAGAGAGCCA GTTAGTCTAC 
GATGGACTAC GCTTCAACCT GCTGGCTCAC AGCAAGCAAA GTGAACCTCT AGATAGATGT 
TTGTCTCTAT GGCGGAGTCT CCTCAGAGGG TCTGTGCCTG CAGTTAGAGA CCCTAAACTC 
ACAGCTTCTT CCATGACTTT GATGGCTGCT CTATACACAC TTATGGGCAA ACACCTGCAA 
GCTCTGGAGG GATATCAGCT TGCTGCCACC CTTTTCCATG TACTTGGTGA TGGTCAGAAT 
AGTGCCAATG CCTTCTGCCA CTCTACCAGG ATCTTGCTTT ACCTGGGTTC ACCGCAGCTT 
GCACAGGTGG ACTTAGAAAA AGCAGAAAAG AGCTTGTCAT CCGTCACCAT CTCTGAGGGC 
ACATCTATTA TCTCCATGCT GGCCATGTTG CAAAAAGCAC AGCTACACTA TGCTCTAGGA 
CAGGTGGAGA GTGGCGTGTC CTGCCTGATG GAGGTGATAA AGGAATCTGC TCAGCATCAT 
TCTAAGAGCT GGTACCTGCT CAGGGCTCGA GCACTGCAGA CAGCCAGTGA ATACTTGAGT 
CTGGACACAC TGGCTTTGAA CTCACAGCTA CGCCAGATCA TCATGCAGCA GGGTCTAAAG 
ACTCCAGACA CTGCTCAATA TGAAGGGTTG AAGCTGTTGT GTAGCCTGGT GATGATGTTA 
TTGGGCAACG GGTTTTACGG AGCGCCTGGA CCAAACACTG ACACTCGCTT TGTGGATAAA 
GGAGACAGTG TCGTGTTTAA GTGGTTGTTG TTGAGTGAAG TGCTGGTGTG CTCGGAGAGA 
ATGGTGATCA CCAGGAGCAG CAGCGGAGCC GTTCATGAAG CTAAAGCTCA GTGTCTAGAG 
GCTCTCAAAC TGGCCACCAA ACTGCAGACA CTCAGCCACT GTGCTGAGCT GTTGGTGCTG 
AAGGCAGAGC TGGAGCTGAT GAAGGGTGCT ACTGAAGCTA GTAGTTTAGA CCTGGAGCAA 
GTTAGACATT TACTTGATCT CTGCACAGAT TTTGGCCAGG GACGACAACA GAAATCTGAA 
GTGAAAATCA AACCACGCAA AGGTCGTCCC GCTGCCCCTT CTTCCACTGG TCACATCTTT 
GAGGAAGAGG ATGATCTGAG TGGGATCCTC AGCTCTCGTG CCCTTCCGAA GGAGCCTGTG 
GAGAACATGG CTAGATTAGG AAGCCAAGGA GCTTCTCCCC CTCTAAAGCC CAAACGCCAG 
CATGGCCTCT CCTGTCTGAC CCATGAAGAG ACCTGCTCTT GTCTCTGCTG CACTGAGCTC 
AGCGTGGCTC GAGTCAGCAT CCACTGGGCA CTATTACAGG CAGATCTCCA GACAGATCCC 
GAACGCTCTC TCAGACTACG CCAGTCTGCC AGGAAACGCT GCCGCTATGT TGTAACTAAA 
CTTCAGAGAA GTTTGGCAGC ACTTGTACAC TCCAAAAAAT CACCTAATGT CTGCTTGTCA 
CTGCTTCAGG CTGAACAGGG TAAAGTGCAT CTGGGTGCTG TGCTTCAACT GCTCAGAACT 
GGAGACAAAG GGAAAGCTGC CATCTTGTGG GAGGAGATAG AAGCAGGATT AGAGGCAGTG 
ACACCTAAAG GAGCACTGAC ACCAGAACTT GGGCCTATCA GAGCTGCTCT ACTGGGGGCT 
AAAGCCGTAG CCTGCTGCTT GGCGTTGGCC ATGAAGAAAC AGTGTATACC CGAAGAGCTG 
TTTTCTTCAT TGTGGCACTG GAATCCAACA AAGGTTAAAC CGCAATTAAA ATCTAAGCAT 
CGATCCAAGA GTCCGGACGC AGCTCCTCTA GAGCCAGGTG TTAAACATAA CCCTAAAGTT 
AAGAGTGGAC TTGAGCAGAA TGAAAGCAGA AAAAAAGAGA CCACTGTCAT TTCGAAGAAA 
GCTAAAGACT CTGTGCCCAA AATTACCATC ACAAAATCCT CCATGGTTTT TAAAACTCCC 
AAAGCAACGA GAACAACCCG GCCCAAATCG GTCTCCACTA GCACTGGCAT CAGTGACCTG 
AGAGCATTTG ACTTCAATAA TGAGGTGCCT GACATTTCAG TCAACTTCAC ACCATCATTA 
CACCGATCTA CAAACCAAAG TGCGACTGCA AAGTCAAAAG CGTCCTCAAA GGGGTCGTTC 
CAGGTTTACA AAGATTCTTC TCCTGCTGCA GAGAAAGCTG TAGTTGTCCC GGCTGCTCCT 
AAACGAACAA AGCGTTCTCG CTTCAAGGTT GAGTTCAGCG ATGAAAGCGA CAACGATGCT 
GCTCCTCTTG CTGTAATGGA AAAGTCAGAG AAAAAGAAAA ACACCTTTAA AACATCACGC 
ACCTTGAAAC CAACCCTTAA CACCATCCCT AGTGACACCA AACTTGTGAG CTCCACAGTA 
GATCCAGAAC CCTCCAGACG AACACGGACC ACTAAGAAAA GCACAGCTCT TCCCTCCATT 
GGTGGCCTCT CATCTGAGGA AGAACTTGGA GTATCTTCAC AGACACGTAC GCGTAGAGGA 
CGACCAAAGA AGAGCACCAG CTCAGCAGAC ACAGAGGAGC CAGAGAGGAT GAGGATGATT 
AAGGAGGAAG AACAGAGGGT TCTTTTGAAT GTCAGCATGG AGGAGCTCAG AGGCTCTGAC 
ACCGAGACAA AAGACACTGG AAGTCCAGAT GCTGACTGTG AGGTCTTGAG GAGAGATCTA 
GGTGCAGATT TTGGGCAAGA GTGTTTCAGT GAGCTCAGGA GAGCTGGACA GAATGGATCA 
GGATCTCACA CAGCTCTGCC ACATGCAACT ACATCTCCTG ACTTGTCAGT AGAGACGGTC 
CAGTCATACC TGCGCTCCTC ATGGCTCCTC CTGCACCACT TCCCGTTTCC TTCTCTTTAT 
CCTCACATCT GCTCATTACT CGCTCAGTCA TTCGGTCAAA CGGACCCCAT CATCACAGCA 
ATGCTGCATG CACAGGCACT GGGAGTGTCC ACTCGCCACC ATATGACCCG TCACGTGGTT 
AACCAGTTCA GGAAGTTGAA GAAATCCTGT AACGATGTAG CAGAAGGTCT TGGAGCTCTG 
AGTCTGGAGG AAACATCTGG GGCAACGCAG TCAAAGACAC TCTCTGCGTT GGAGCAGATC 
TTTTCATTCA CAGCAGCAAA CCCAATGCAG TTTCCACAGA CACACTGTCA GCAGTTCACA 
CAGCAGCTGG AAGATCTACC TGCAGGAGTG ACCGTATGCT TGCTGTCACT GACGGGGGTG 
CATCCTGATG AAATTGGCAA CACTATTCTT CTGACCCGAC TGGAGCGCGG CTCTACCCCA 
ATCACAGTTC GAATCCCCAC TGCAGACAGA GAGCAATCTG TTGCTGGCCT GTTGGAAGAA 
ATGGATGGAG TCTTGAAAGG ACAAAAGGAA GTGAGCACAG TGGCTGAGAA ATCACAGTGG 
TGGGACGGAA GGAAGGCTTT AGATGCCCGC GTGGAGAAAA TGCTGGAGGA GATGGAAGAG 
GCTTTAGGAG TGTGGCGAAC TCTTCTTCTG CCTTTGACCT CTGATCCTAA GCTTGATGTT 
CAGGTAAAGA GCCTCCAAAA GGCTCTTAAA TCAACAAAGA TCACACGGGA CACTCTCAAG 
GTGATTCTTT CAGCGTCTCC ACTGCTGTCC CTGCCAGACC TGCAGTGTCT TCTGGAAGGA 
ATGAATGTGC AGGACAAGGA CCTGCTGAAG CTCCTGCAGA GGGCCGTGGC TGAACTGAGA 
GGGAGGGAGG AGCAACAGGG ACACACTGTT CTGATTTTAG ACAAGTACTT GCAGAGGTTG 
CCGTGGGAGA ACATTGCTTG TCTGAAGTCT CAGTCGGTCA CTCGAATGCC CTCATTACAC 
GCGGTGCTTG GACACAGTCA TCTGAGAGAG GTGGATACAA GTTGTGTTCT GTCTTGTGGT 
GTGAACCCTA AGCAGGTTTA CTATGTGCTA AACCCAGATG GAAACCTGCC CGACACTGAG 
AATCGCTTCA AAGAGTGGTT TACAGGTGAA CGAACATGGC AGGGGGTTTG TGGAAGGGCT 
CCTGATCCAG AGAAACTACA AGAGGCTGTG ACCACTAAGG ATCTCTACAT ATACATCGGA 
CATGGTGCAG GAGCCCGCTT TCTGGATGCG CAGAGAGTTT TGAAGGTGCC TGTGAGGGCC 
GCAGCTCTTC TGTTCGGCTG CAGTAGTGCT GCTCTCAGTG TGCTTGGTCA GCAGGAGGGA 
ACGGGCATCA TCCTCAGCTA CCTGACCGCT GGATGTCCAT TGGTGCTGGG TAACCTGTGG 
GATGTGACAG ATCGTGACTT GGATCGATTC ACATCGGCTC TGCTCCAGTC TTGGCTCTCT 
GCTGGTTCTG GCTCTTCACT TCTTCAGCAT TTGGCGAAAT CACGCAGCGC AACTCATTTA 
AAACATATAA TCGGTGCTGC GCCCATTGCG TATGGTTTAC CGGTGCATAT ACACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

espl1 ( XM_021474123.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021329798.1 Danio rerio extra spindle pole bodies like 1, separase (espl1), transcript variant X1, mRNA.

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CloneID	ODa146569
Clone ID Related Accession (Same CDS sequence)	XM_021474123.1
Accession Version	XM_021474123.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6546bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	separin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395261.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCATTAA AGAGCTTGAA AACAGAAGAT TACGTTCAGC AGTTATCCTG TGTCAAGGAC 
ACCATAGTCC TCCACAACGA ACTGAAGAAA CATGTGGATG GTGGCTTGGG TCCTTATGGT 
CGCACAATGT GTGATCGTAT CATCAGAGCC TGCAACCAGC GGCTGGGCAA CGGCTCTGTG 
GATGCTGGCC TTCAGGAGCG GATAGTGGAG CTGGTGGAAC TGGCTGTCCA AGGGTTTCAA 
CTGCTTGAAG AGTCCGGCAT GCAGAGCACC CCCTTTTATC TGGAGAAAAT AGTCTTTCAC 
ATCCTACAGA AAGTCGCAAG TCTTGGAGCT CATGGTCCTG CTTGTCGACT GGGTCAGCTC 
GTGTATAGAA GACTGAAATG TTTGTCTGCA GAGATTGACG ACTTTAATGT CCTGGTGCGG 
AATTGCTTTG CAGTCTTGTG GAACAGTCTC CTCTCTAACA AACCGAGTGC TTCCACCCTC 
CCTCCACGAG ACAGACTCCA CTGTCAGCTG CAAGCTGTAA TATTCAGACT CCTTGAACAG 
GGCTCCAGCT TGTCCTCTTC CCCATCCAAA GTTCCTCTGT TTGTTGAGGA AGCAGTAGTG 
GAGTACGAAC GATCATGTGG AGGTTTTACT CAAGACGATG TGAGTTTTCT GACCTCTGAA 
TTGCGTGAGC TGTTCTCTGG TGCTTTATTC AATCCTCAGG ACTCTGCATC AACTTCTTCA 
CTGCTGGCTG TGTGGTGTGA GGTGGCATTT AAAGTGTCTA AACTGCTCTG CAAAAGTGGC 
TTTTCACCTG AGGCTGTTGA ACTCTTGCGA GGCACTCTGA AGGAGGTTAA TGGCCACGTT 
GGCCTGCGTT CGGCTCTGGG TCTGGCAGAC CGGGCAGTGC AGATGCAGTG TGTGTTAAGT 
TCTGGAGGTG AATGCAGTAA GAGCTTTACT GAATGTGCCC GTGCTCTCCG GATGTTGCCT 
TGTGCCATGT CCAGCTCAGA GTCTCATGCC CTGTTAGAGG CTTGTCAGCT GGTGGTTTGG 
ACGTTGGAAG CTAGTCAGTG TAAAGGAATG GATGTGACCA CTTTGCTGGC TTGTTTTTCC 
TTTTTGGAAG AATATCAGGA ATTTCTCCCT ACACAGCAAA AGAACTCGTT TTCGCTGCAG 
GTGCAATACT CTCTTTGTTT CAGTTTTTAC CAGGGCTTCA TCTGCACGTA TGATAGTCTT 
CATGCATCTC AGGTGTCTGT GGGCGAGACC TTGGACAGAG TGCTATTGTT CTGCCGGTCG 
ACCGCAGGAC GTATGCTTGC TGAACTGCGG ACACTCAATA ATGACAACTT GCTGCTTAAA 
GCAGTGTGTG CAGTGAATAA TGTGGTGTAC GAGCTGTTTA ACAGGAAGCT GTATGAAGGA 
GCCTTTGGAT TGTCGATGAT TGTGTGTCAG GAGCTCTGTA AGGACTGCCC GCCCTCTCTT 
CCTGTGGATC GGGTCAACCG CTGTTTCATG CTGACAGTGC AGTGTAGTCG CCGCGCAGGC 
CATCTGGAGA AGGCTCTGGA TTGGATTGTT CGCTGGATTC AGGCTTTGGG CTCACAGATT 
TTGGATCATC TCACTGAACC TGTGTCGATG TGGGTCAAAA CTAAGTGTGA TGCCGCCAGA 
GCAGGCGAGG ATGACACACG CCTGAGGACT CTGAGGGACG GGTTAGGAGA ATCCTTAGTG 
GATGAGGAGG TTATGGTGTG TTTGTTAGAG GAGGAGTTGC GTGTATATAA GGAGCAGACT 
GGAGACACAG CACAGGAACG CTACAACACA CTCTGCGACC TCTTGGAAAT CTGCCATGAA 
GAAACCCCAC GCACACTAAG AAGAGCTACG TATCTGTGTG AGATGGCGCA GGTTGTGTGC 
TATCAGGACT TCAGCCAGCA GACTGACTGC TCAGCAGTTG ATTTTACCCA CGAGGCTTTG 
CGGTTGCTGG AAGCAGAACC AGAGACGGCA GAGAATACTG ATAGGCTGAA GGATGAGAAA 
GCCCACGCTT CCCTGTGGCT CTATATCTGC ACTCTTGAGT CTAACCTGCA GGAGGCTGTG 
GACACAGAGA AGCGTTTGTG TGCAGTGCAG GAGGAGAATA AAAGAGATGT GAATATGGAT 
CCTGTTCCCA CAAATGATCT GGAGTACGAG GACAAACAGA AATCACAAGA GAGCCAGTTA 
GTCTACGATG GACTACGCTT CAACCTGCTG GCTCACAGCA AGCAAAGTGA ACCTCTAGAT 
AGATGTTTGT CTCTATGGCG GAGTCTCCTC AGAGGGTCTG TGCCTGCAGT TAGAGACCCT 
AAACTCACAG CTTCTTCCAT GACTTTGATG GCTGCTCTAT ACACACTTAT GGGCAAACAC 
CTGCAAGCTC TGGAGGGATA TCAGCTTGCT GCCACCCTTT TCCATGTACT TGGTGATGGT 
CAGAATAGTG CCAATGCCTT CTGCCACTCT ACCAGGATCT TGCTTTACCT GGGTTCGCCG 
CAGCTTGCAC AGGTGGACTT AGAAAAAGCA GAAAAGAGCT TGTCATCCGT CACCATCTCT 
GAGGGCACAT CTATTATCTC CATGCTGGCC ATGTTGCAAA AAGCACAGCT ACACTATGCT 
CTAGGACAGG TGGAGAGTGG CGTGTCCTGC CTGATGGAGG TGATAAAGGA ATCTGCTCAG 
CATCATTCTA AGAGCTGGTA CCTCCTCAGG GCTCGAGCAC TGCAGACAGC CAGTGAATAC 
TTGAGTCTGG ACACACTGGC TTTGAACTCA CAGCTACGCC AGATCATCAT GCAGCAGGGT 
CTAAAGACTC CAGACACTGC TCAATATGAA GGGTTGAAGC TGTTGTGTAG CCTGGTGATG 
ATGTTATTGG GCAACGGGTT TTACGGAGCG CCTGGACCAA ACACTGACAC TCGCTTTGTG 
GATAAAGGAG ACAGTGTTGT GTTTAAGTGG TTGTTGCTGA GTGAAGTGCT GGTGTGCTCG 
GAGAGAATGG TGATCACCAG GAGCAGCAGC GGAGCCGTTC ATGAAGCTAA AGCTCAGTGT 
CTAGAGGCTC TCAAACTGGC CACCAAACTG CAGACACTCA GCCACTGTGC TGAGCTGTTG 
GTGCTGAAGG CAGAGCTGGA GCTGATGAAG GGTGCTACTG AAGCTAGTAG TTTAGACCTG 
GAGCAAGTTA GACATTTACT TGATCTCTGC ACAGATTTTG GCCAGGGACG ACAACAGAAA 
TCTGAAGTGA AAATCAAACC ACGCAAAGGT CGTCCCGCTG CCCCTTCTTC CTCTGGTCAC 
ATCTCTGAGG AAGAGGATGA TCTGAGTGGG ATCCTCAGCT CTCGTGCCCT TCCGAAGGAG 
CCTGTGGAGA ACATGGCTAG ATTAGGAAGC CAAGGAGCTT CTCCCCCTCT AAAGCCCAAA 
CGCCAGCATG GCCTCTCCTG TCTGACCCAT GAAGAGACCT GCTCTTGTCT CTGCTGCACT 
GAGCTCAGCG TGGCTCGAGT CAGCATCCAC TGGGCACTAT TACAGGCAGA TCTCCAGACA 
GATCCCGAAC ACTCTCTCAG ACTACGCCAG TCTGCCAGGA AACGCTGCCG CTATGTTGTA 
ACTAAACTTC AGAGAAGTTT GGCAGCACTT GTACACTTCA AAAAATCAAC TAATGTCTGC 
TTGTCACTGC TTCAGGCTGA ACAGGGTAAA GTGCATCTGG GTGCTGTGCT TCAACTGCTC 
AGAACTGGAG ACAAAGGGAA AGCTGCCATC TTGTGGGAGG AGATAGAAGC AGGATTAGAG 
GCAGTGACAC CTAAAGGAGC ACTGACACCA GAACTTGGGC CTATCAGAGC TGCTCTACTG 
GGGGCTAAAG CCGTAGCCTG CTGCTTGGCG TTGGCCATGA AGAAACAGTG TATACCCGAA 
GAGCTGTTTT CTTCATTGTG GCACTGGAAT GCAACAAAGG TTAAACCGCA ATTAAAATCT 
AAGCATCGAT CCAAGAGTCC GGACGCAGCT CCTCTAGAGC CAGGTGTTAA ACATAACCCT 
AAAGTTAAGA GTGGACTTGA GGAGAATGAA AGCAGAAAAA AAGAGACCAC TGTCATTTCG 
AAGAAAGCTA AAGACTCTGT GCCCAAGATT ACTATTACAA AATCCTCCAT GGTTTTTAAA 
ACGCCCAAAG CAACGAGAAC AACCCGGCCC AAATCTGTCT CCACTAGCAC TGGCATCAGT 
GACCTGAGAG CATTTGACTT CAATAATGAG GTGCCTGACA TTTCAGTCAA CTTCACACCA 
TCATTACACC GATCTACAAA CCAAAGTGCG ACTGCAAAGT CAAAAGCGTC CTCAAAGGGG 
TCGTTCCAGG TTTACAAAGA TTCTTCTCCT GCTGCAGAGA AAGCTGTAGT TGTCCCGGCT 
GCTCCTAAAC GAACAAAGCG TTCTCGCTTC AAGGTTGAGT TCAGCGATGA AAGCGACACC 
GAAGCTGCTC CTCTTGCTGT AATGGAAAAG TCAGAGAAAA AGAAAAACAC CTCTAAAACA 
TCACGCACCT TGAAACCAAC CCTTAACACC ATCCCTAGTG ACACCAAACT TTTGAGCTCC 
ACAGTAGATC CAGAACCCTC CAGACGAACA CGGACCACTA AGAAAAGCAC AGCTCTTCCC 
TCCATTGGTG GCCTCTCTTC TGAGGAAGAA CTTGGAGTAT CTTCACAGAC ACGTACGCGT 
AGAGGACGAC CAAAGAAGAG CACCAGCTCA GCAGACACAG AGGAGCCAGA GAGGATGAGG 
ATGATTAAGG AGGAAGAACA GAGGGTTCTT TTGAATGTCA GCATGGAGGA GCTCAGAGGC 
TCTGACACCG AGACAAAAGA CACTGGAAGT CCAGATGCTG ACTGTGAGGT CTTGAGGAGA 
GATCTAGGTG CAGATTTTGG GCGAGAGTGT TTCAGTGAGC TCAGGAGAGC TGGACAGAAT 
GGATCAGGAT CTCACACAGC TCTGCCACAT GCAACTACAT CTCCTGCAGA CTTGTCAGTA 
GAGACGGTCC AGTCATACCT GCGCTCCTCA TGGCTCCTCC TGCACCACTT CCCGTTTCCT 
TCTCTTTATC CTCACATCTG CTCATTACTC GCTCAGTCAT TCGGTCAAAC GGACCCCATC 
ATCACAGCAA TGCTGCATGC ACAGGCACTG GGAGTGTCCA CTCGCCACCA TATGACCCGT 
CACGTGGTTA ACCAGTTCAG AAAGTTGAAG AAATCCTGTA ACGATGTAGC AGAAGGTCTT 
GGAGCTCTGA GTCTGGAGGA AACATCTGGA GCAACGCAGT CAAAGAAACT CTCTGCGTTG 
GAGCAGATCT TTTCATTCAC AGCATCAAAC CCAATGCAGT TTCCACAGAC ACACTGTCAG 
CAGTTCACAC AGCAGCTGGA AGATCTACCT GCAGGAGTGA CTGTATGCTT GCTGTCACTG 
ACGGGGGTGC ATCCTGATGA AATTGGCAAC ACTATTCTTC TGACCCGACT GGAGCGCGGC 
TCTACCCCAA TCACAGTTCG AATCCCCACT GCAGACAGAG AGCAATCTGT TGCTGGCCTG 
TTGGAAGAAA TGGATGGAGT CTTGAAAGGA CAAAAGGAAG TGAGCACAGT GGCTGAGAAA 
TCACAGTGGT GGGACGGAAG GAAGGCTTTA GATGCCCGTG TGGAGAAAAT GCTGGAGGAG 
ATGGAAGAGG CTTTAGGAGT GTGGCGAACT CTTCTTCTGC CTTTGACCTC TGATCCTAAG 
CTTGATGTAC AGGTAAAGAG CCTCCAAAAG GCTCTTAAAT CAACAAAGAT CACACAGGAT 
ACTCTCAAGG TGATTCTTTC AGCCTCTCCA CTGCTGTCCC TGCCAGACCT GCAGTGTCTT 
CTGGAAGGAA TGAATGTGCA GGACAAGGAC CTGCTGAAGC TCCTTCAGAG AGGCGTGGCT 
GAACTGAGAG GGAGGGAGGA GCCACAGGGA CACACTGTTC TGATTTTAGA CAAGTACTTG 
CAGAGGTTGC CGTGGGAGAA CATTGCTTGT CTGAAGTCTC AGTCGGTCAC TCGAATGCCC 
TCATTACATG CGGTGCTTGG ACACAGTCAT CTGAGAGAGG TGGATACAAG TTGTGTTCTG 
TCTTGTGGTG TGAACCCTAA GCAGGTTTAC TATGTGCTAA ACCCAGATGG AAACCTGCCC 
GACACTGAGA ATCGCTTCAA AGAGTGGTTT ACAGGTGAAC GAACATGGCA GGGGGTTTGT 
GGAAGGGCTC CTGATCCGGA GAAACTACAA GAGGCTGTGA CCACTAAAGA TCTCTACATA 
TACATCGGAC ATGGTGCAGG AGCCCGCTTT CTGGATGCGC AGAGAGTTTT GAAGGTGCCT 
GTGAGGGCCG CAGCTCTTCT GTTCGGCTGC AGTAGTGCTG CTCTCAGTGT GCTTGGTCAG 
CAGGAGGGAA CGGGCATCAT CCTCAGCTAC CTGACCGCTG GATGTCCAAT GGTGCTGGGT 
AACCTGTGGG ATGTGACAGA TCGTGACTTG GATCGATTCA CATCGGCTCT GCTCCAGTCT 
TGGCTCTCTG CTGGTTCTGG CTCTTCACTT CTTCAGCATT TGGCGAAATC ACGCAGCGCA 
ACTCATTTAA AACACATAAT CGGTGCTGCG CCCATTGCGT ATGGTTTACC GGTGCATATA 
CACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021329798.1
CDS	221..6766
Translation	MALKSLKTEDYVQQLSCVKDTIVLHNELKKHVDGGLGPYGRTMCDRIIRACNQRLGNGSVDAGLQERIVELVELAVQGFQLLEESGMQSTPFYLEKIVFHILQKVASLGAHGPACRLGQLVYRRLKCLSAEIDDFNVLVRNCFAVLWNSLLSNKPSASTLPPRDRLHCQLQAVIFRLLEQGSSLSSSPSKVPLFVEEAVVEYERSCGGFTQDDVSFLTSELRELFSGALFNPQDSASTSSLLAVWCEVAFKVSKLLCKSGFSPEAVELLRGTLKEVNGHVGLRSALGLADRAVQMQCVLSSGGECSKSFTECARALRMLPCAMSSSESHALLEACQLVVWTLEASQCKGMDVTTLLACFSFLEEYQEFLPTQQKNSFSLQVQYSLCFSFYQGFICTYDSLHASQVSVGETLDRVLLFCRSTAGRMLAELRTLNNDNLLLKAVCAVNNVVYELFNRKLYEGAFGLSMIVCQELCKDCPPSLPVDRVNRCFMLTVQCSRRAGHLEKALDWIVRWIQALGSQILDHLTEPVSMWVKTKCDAARAGEDDTRLRTLRDGLGESLVDEEVMVCLLEEELRVYKEQTGDTAQERYNTLCDLLEICHEETPRTLRRATYLCEMAQVVCYQDFSQQTDCSAVDFTHEALRLLEAEPETAENTDRLKDEKAHASLWLYICTLESNLQEAVDTEKRLCAVQEENKRDVNMDPVPTNDLEYEDKQKSQESQLVYDGLRFNLLAHSKQSEPLDRCLSLWRSLLRGSVPAVRDPKLTASSMTLMAALYTLMGKHLQALEGYQLAATLFHVLGDGQNSANAFCHSTRILLYLGSPQLAQVDLEKAEKSLSSVTISEGTSIISMLAMLQKAQLHYALGQVESGVSCLMEVIKESAQHHSKSWYLLRARALQTASEYLSLDTLALNSQLRQIIMQQGLKTPDTAQYEGLKLLCSLVMMLLGNGFYGAPGPNTDTRFVDKGDSVVFKWLLLSEVLVCSERMVITRSSSGAVHEAKAQCLEALKLATKLQTLSHCAELLVLKAELELMKGATEASSLDLEQVRHLLDLCTDFGQGRQQKSEVKIKPRKGRPAAPSSSGHISEEEDDLSGILSSRALPKEPVENMARLGSQGASPPLKPKRQHGLSCLTHEETCSCLCCTELSVARVSIHWALLQADLQTDPEHSLRLRQSARKRCRYVVTKLQRSLAALVHFKKSTNVCLSLLQAEQGKVHLGAVLQLLRTGDKGKAAILWEEIEAGLEAVTPKGALTPELGPIRAALLGAKAVACCLALAMKKQCIPEELFSSLWHWNATKVKPQLKSKHRSKSPDAAPLEPGVKHNPKVKSGLEENESRKKETTVISKKAKDSVPKITITKSSMVFKTPKATRTTRPKSVSTSTGISDLRAFDFNNEVPDISVNFTPSLHRSTNQSATAKSKASSKGSFQVYKDSSPAAEKAVVVPAAPKRTKRSRFKVEFSDESDTEAAPLAVMEKSEKKKNTSKTSRTLKPTLNTIPSDTKLLSSTVDPEPSRRTRTTKKSTALPSIGGLSSEEELGVSSQTRTRRGRPKKSTSSADTEEPERMRMIKEEEQRVLLNVSMEELRGSDTETKDTGSPDADCEVLRRDLGADFGRECFSELRRAGQNGSGSHTALPHATTSPADLSVETVQSYLRSSWLLLHHFPFPSLYPHICSLLAQSFGQTDPIITAMLHAQALGVSTRHHMTRHVVNQFRKLKKSCNDVAEGLGALSLEETSGATQSKKLSALEQIFSFTASNPMQFPQTHCQQFTQQLEDLPAGVTVCLLSLTGVHPDEIGNTILLTRLERGSTPITVRIPTADREQSVAGLLEEMDGVLKGQKEVSTVAEKSQWWDGRKALDARVEKMLEEMEEALGVWRTLLLPLTSDPKLDVQVKSLQKALKSTKITQDTLKVILSASPLLSLPDLQCLLEGMNVQDKDLLKLLQRGVAELRGREEPQGHTVLILDKYLQRLPWENIACLKSQSVTRMPSLHAVLGHSHLREVDTSCVLSCGVNPKQVYYVLNPDGNLPDTENRFKEWFTGERTWQGVCGRAPDPEKLQEAVTTKDLYIYIGHGAGARFLDAQRVLKVPVRAAALLFGCSSAALSVLGQQEGTGIILSYLTAGCPMVLGNLWDVTDRDLDRFTSALLQSWLSAGSGSSLLQHLAKSRSATHLKHIIGAAPIAYGLPVHIH

Target ORF information:

RefSeq Version	XM_021474123.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio extra spindle pole bodies like 1, separase (espl1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021474123.1

ORF Insert Sequence:

ATGGCATTAA AGAGCTTGAA AACAGAAGAT TACGTTCAGC AGTTATCCTG TGTCAAGGAC 
ACCATAGTCC TCCACAACGA ACTGAAGAAA CATGTGGATG GTGGCTTGGG TCCTTATGGT 
CGCACAATGT GTGATCGTAT CATCAGAGCC TGCAACCAGC GGCTGGGCAA CGGCTCTGTG 
GATGCTGGCC TTCAGGAGCG GATAGTGGAG CTGGTGGAAC TGGCTGTCCA AGGGTTTCAA 
CTGCTTGAAG AGTCCGGCAT GCAGAGCACC CCCTTTTATC TGGAGAAAAT AGTCTTTCAC 
ATCCTACAGA AAGTCGCAAG TCTTGGAGCT CATGGTCCTG CTTGTCGACT GGGTCAGCTC 
GTGTATAGAA GACTGAAATG TTTGTCTGCA GAGATTGACG ACTTTAATGT CCTGGTGCGG 
AATTGCTTTG CAGTCTTGTG GAACAGTCTC CTCTCTAACA AACCGAGTGC TTCCACCCTC 
CCTCCACGAG ACAGACTCCA CTGTCAGCTG CAAGCTGTAA TATTCAGACT CCTTGAACAG 
GGCTCCAGCT TGTCCTCTTC CCCATCCAAA GTTCCTCTGT TTGTTGAGGA AGCAGTAGTG 
GAGTACGAAC GATCATGTGG AGGTTTTACT CAAGACGATG TGAGTTTTCT GACCTCTGAA 
TTGCGTGAGC TGTTCTCTGG TGCTTTATTC AATCCTCAGG ACTCTGCATC AACTTCTTCA 
CTGCTGGCTG TGTGGTGTGA GGTGGCATTT AAAGTGTCTA AACTGCTCTG CAAAAGTGGC 
TTTTCACCTG AGGCTGTTGA ACTCTTGCGA GGCACTCTGA AGGAGGTTAA TGGCCACGTT 
GGCCTGCGTT CGGCTCTGGG TCTGGCAGAC CGGGCAGTGC AGATGCAGTG TGTGTTAAGT 
TCTGGAGGTG AATGCAGTAA GAGCTTTACT GAATGTGCCC GTGCTCTCCG GATGTTGCCT 
TGTGCCATGT CCAGCTCAGA GTCTCATGCC CTGTTAGAGG CTTGTCAGCT GGTGGTTTGG 
ACGTTGGAAG CTAGTCAGTG TAAAGGAATG GATGTGACCA CTTTGCTGGC TTGTTTTTCC 
TTTTTGGAAG AATATCAGGA ATTTCTCCCT ACACAGCAAA AGAACTCGTT TTCGCTGCAG 
GTGCAATACT CTCTTTGTTT CAGTTTTTAC CAGGGCTTCA TCTGCACGTA TGATAGTCTT 
CATGCATCTC AGGTGTCTGT GGGCGAGACC TTGGACAGAG TGCTATTGTT CTGCCGGTCG 
ACCGCAGGAC GTATGCTTGC TGAACTGCGG ACACTCAATA ATGACAACTT GCTGCTTAAA 
GCAGTGTGTG CAGTGAATAA TGTGGTGTAC GAGCTGTTTA ACAGGAAGCT GTATGAAGGA 
GCCTTTGGAT TGTCGATGAT TGTGTGTCAG GAGCTCTGTA AGGACTGCCC GCCCTCTCTT 
CCTGTGGATC GGGTCAACCG CTGTTTCATG CTGACAGTGC AGTGTAGTCG CCGCGCAGGC 
CATCTGGAGA AGGCTCTGGA TTGGATTGTT CGCTGGATTC AGGCTTTGGG CTCACAGATT 
TTGGATCATC TCACTGAACC TGTGTCGATG TGGGTCAAAA CTAAGTGTGA TGCCGCCAGA 
GCAGGCGAGG ATGACACACG CCTGAGGACT CTGAGGGACG GGTTAGGAGA ATCCTTAGTG 
GATGAGGAGG TTATGGTGTG TTTGTTAGAG GAGGAGTTGC GTGTATATAA GGAGCAGACT 
GGAGACACAG CACAGGAACG CTACAACACA CTCTGCGACC TCTTGGAAAT CTGCCATGAA 
GAAACCCCAC GCACACTAAG AAGAGCTACG TATCTGTGTG AGATGGCGCA GGTTGTGTGC 
TATCAGGACT TCAGCCAGCA GACTGACTGC TCAGCAGTTG ATTTTACCCA CGAGGCTTTG 
CGGTTGCTGG AAGCAGAACC AGAGACGGCA GAGAATACTG ATAGGCTGAA GGATGAGAAA 
GCCCACGCTT CCCTGTGGCT CTATATCTGC ACTCTTGAGT CTAACCTGCA GGAGGCTGTG 
GACACAGAGA AGCGTTTGTG TGCAGTGCAG GAGGAGAATA AAAGAGATGT GAATATGGAT 
CCTGTTCCCA CAAATGATCT GGAGTACGAG GACAAACAGA AATCACAAGA GAGCCAGTTA 
GTCTACGATG GACTACGCTT CAACCTGCTG GCTCACAGCA AGCAAAGTGA ACCTCTAGAT 
AGATGTTTGT CTCTATGGCG GAGTCTCCTC AGAGGGTCTG TGCCTGCAGT TAGAGACCCT 
AAACTCACAG CTTCTTCCAT GACTTTGATG GCTGCTCTAT ACACACTTAT GGGCAAACAC 
CTGCAAGCTC TGGAGGGATA TCAGCTTGCT GCCACCCTTT TCCATGTACT TGGTGATGGT 
CAGAATAGTG CCAATGCCTT CTGCCACTCT ACCAGGATCT TGCTTTACCT GGGTTCGCCG 
CAGCTTGCAC AGGTGGACTT AGAAAAAGCA GAAAAGAGCT TGTCATCCGT CACCATCTCT 
GAGGGCACAT CTATTATCTC CATGCTGGCC ATGTTGCAAA AAGCACAGCT ACACTATGCT 
CTAGGACAGG TGGAGAGTGG CGTGTCCTGC CTGATGGAGG TGATAAAGGA ATCTGCTCAG 
CATCATTCTA AGAGCTGGTA CCTCCTCAGG GCTCGAGCAC TGCAGACAGC CAGTGAATAC 
TTGAGTCTGG ACACACTGGC TTTGAACTCA CAGCTACGCC AGATCATCAT GCAGCAGGGT 
CTAAAGACTC CAGACACTGC TCAATATGAA GGGTTGAAGC TGTTGTGTAG CCTGGTGATG 
ATGTTATTGG GCAACGGGTT TTACGGAGCG CCTGGACCAA ACACTGACAC TCGCTTTGTG 
GATAAAGGAG ACAGTGTTGT GTTTAAGTGG TTGTTGCTGA GTGAAGTGCT GGTGTGCTCG 
GAGAGAATGG TGATCACCAG GAGCAGCAGC GGAGCCGTTC ATGAAGCTAA AGCTCAGTGT 
CTAGAGGCTC TCAAACTGGC CACCAAACTG CAGACACTCA GCCACTGTGC TGAGCTGTTG 
GTGCTGAAGG CAGAGCTGGA GCTGATGAAG GGTGCTACTG AAGCTAGTAG TTTAGACCTG 
GAGCAAGTTA GACATTTACT TGATCTCTGC ACAGATTTTG GCCAGGGACG ACAACAGAAA 
TCTGAAGTGA AAATCAAACC ACGCAAAGGT CGTCCCGCTG CCCCTTCTTC CTCTGGTCAC 
ATCTCTGAGG AAGAGGATGA TCTGAGTGGG ATCCTCAGCT CTCGTGCCCT TCCGAAGGAG 
CCTGTGGAGA ACATGGCTAG ATTAGGAAGC CAAGGAGCTT CTCCCCCTCT AAAGCCCAAA 
CGCCAGCATG GCCTCTCCTG TCTGACCCAT GAAGAGACCT GCTCTTGTCT CTGCTGCACT 
GAGCTCAGCG TGGCTCGAGT CAGCATCCAC TGGGCACTAT TACAGGCAGA TCTCCAGACA 
GATCCCGAAC ACTCTCTCAG ACTACGCCAG TCTGCCAGGA AACGCTGCCG CTATGTTGTA 
ACTAAACTTC AGAGAAGTTT GGCAGCACTT GTACACTTCA AAAAATCAAC TAATGTCTGC 
TTGTCACTGC TTCAGGCTGA ACAGGGTAAA GTGCATCTGG GTGCTGTGCT TCAACTGCTC 
AGAACTGGAG ACAAAGGGAA AGCTGCCATC TTGTGGGAGG AGATAGAAGC AGGATTAGAG 
GCAGTGACAC CTAAAGGAGC ACTGACACCA GAACTTGGGC CTATCAGAGC TGCTCTACTG 
GGGGCTAAAG CCGTAGCCTG CTGCTTGGCG TTGGCCATGA AGAAACAGTG TATACCCGAA 
GAGCTGTTTT CTTCATTGTG GCACTGGAAT GCAACAAAGG TTAAACCGCA ATTAAAATCT 
AAGCATCGAT CCAAGAGTCC GGACGCAGCT CCTCTAGAGC CAGGTGTTAA ACATAACCCT 
AAAGTTAAGA GTGGACTTGA GGAGAATGAA AGCAGAAAAA AAGAGACCAC TGTCATTTCG 
AAGAAAGCTA AAGACTCTGT GCCCAAGATT ACTATTACAA AATCCTCCAT GGTTTTTAAA 
ACGCCCAAAG CAACGAGAAC AACCCGGCCC AAATCTGTCT CCACTAGCAC TGGCATCAGT 
GACCTGAGAG CATTTGACTT CAATAATGAG GTGCCTGACA TTTCAGTCAA CTTCACACCA 
TCATTACACC GATCTACAAA CCAAAGTGCG ACTGCAAAGT CAAAAGCGTC CTCAAAGGGG 
TCGTTCCAGG TTTACAAAGA TTCTTCTCCT GCTGCAGAGA AAGCTGTAGT TGTCCCGGCT 
GCTCCTAAAC GAACAAAGCG TTCTCGCTTC AAGGTTGAGT TCAGCGATGA AAGCGACACC 
GAAGCTGCTC CTCTTGCTGT AATGGAAAAG TCAGAGAAAA AGAAAAACAC CTCTAAAACA 
TCACGCACCT TGAAACCAAC CCTTAACACC ATCCCTAGTG ACACCAAACT TTTGAGCTCC 
ACAGTAGATC CAGAACCCTC CAGACGAACA CGGACCACTA AGAAAAGCAC AGCTCTTCCC 
TCCATTGGTG GCCTCTCTTC TGAGGAAGAA CTTGGAGTAT CTTCACAGAC ACGTACGCGT 
AGAGGACGAC CAAAGAAGAG CACCAGCTCA GCAGACACAG AGGAGCCAGA GAGGATGAGG 
ATGATTAAGG AGGAAGAACA GAGGGTTCTT TTGAATGTCA GCATGGAGGA GCTCAGAGGC 
TCTGACACCG AGACAAAAGA CACTGGAAGT CCAGATGCTG ACTGTGAGGT CTTGAGGAGA 
GATCTAGGTG CAGATTTTGG GCGAGAGTGT TTCAGTGAGC TCAGGAGAGC TGGACAGAAT 
GGATCAGGAT CTCACACAGC TCTGCCACAT GCAACTACAT CTCCTGCAGA CTTGTCAGTA 
GAGACGGTCC AGTCATACCT GCGCTCCTCA TGGCTCCTCC TGCACCACTT CCCGTTTCCT 
TCTCTTTATC CTCACATCTG CTCATTACTC GCTCAGTCAT TCGGTCAAAC GGACCCCATC 
ATCACAGCAA TGCTGCATGC ACAGGCACTG GGAGTGTCCA CTCGCCACCA TATGACCCGT 
CACGTGGTTA ACCAGTTCAG AAAGTTGAAG AAATCCTGTA ACGATGTAGC AGAAGGTCTT 
GGAGCTCTGA GTCTGGAGGA AACATCTGGA GCAACGCAGT CAAAGAAACT CTCTGCGTTG 
GAGCAGATCT TTTCATTCAC AGCATCAAAC CCAATGCAGT TTCCACAGAC ACACTGTCAG 
CAGTTCACAC AGCAGCTGGA AGATCTACCT GCAGGAGTGA CTGTATGCTT GCTGTCACTG 
ACGGGGGTGC ATCCTGATGA AATTGGCAAC ACTATTCTTC TGACCCGACT GGAGCGCGGC 
TCTACCCCAA TCACAGTTCG AATCCCCACT GCAGACAGAG AGCAATCTGT TGCTGGCCTG 
TTGGAAGAAA TGGATGGAGT CTTGAAAGGA CAAAAGGAAG TGAGCACAGT GGCTGAGAAA 
TCACAGTGGT GGGACGGAAG GAAGGCTTTA GATGCCCGTG TGGAGAAAAT GCTGGAGGAG 
ATGGAAGAGG CTTTAGGAGT GTGGCGAACT CTTCTTCTGC CTTTGACCTC TGATCCTAAG 
CTTGATGTAC AGGTAAAGAG CCTCCAAAAG GCTCTTAAAT CAACAAAGAT CACACAGGAT 
ACTCTCAAGG TGATTCTTTC AGCCTCTCCA CTGCTGTCCC TGCCAGACCT GCAGTGTCTT 
CTGGAAGGAA TGAATGTGCA GGACAAGGAC CTGCTGAAGC TCCTTCAGAG AGGCGTGGCT 
GAACTGAGAG GGAGGGAGGA GCCACAGGGA CACACTGTTC TGATTTTAGA CAAGTACTTG 
CAGAGGTTGC CGTGGGAGAA CATTGCTTGT CTGAAGTCTC AGTCGGTCAC TCGAATGCCC 
TCATTACATG CGGTGCTTGG ACACAGTCAT CTGAGAGAGG TGGATACAAG TTGTGTTCTG 
TCTTGTGGTG TGAACCCTAA GCAGGTTTAC TATGTGCTAA ACCCAGATGG AAACCTGCCC 
GACACTGAGA ATCGCTTCAA AGAGTGGTTT ACAGGTGAAC GAACATGGCA GGGGGTTTGT 
GGAAGGGCTC CTGATCCGGA GAAACTACAA GAGGCTGTGA CCACTAAAGA TCTCTACATA 
TACATCGGAC ATGGTGCAGG AGCCCGCTTT CTGGATGCGC AGAGAGTTTT GAAGGTGCCT 
GTGAGGGCCG CAGCTCTTCT GTTCGGCTGC AGTAGTGCTG CTCTCAGTGT GCTTGGTCAG 
CAGGAGGGAA CGGGCATCAT CCTCAGCTAC CTGACCGCTG GATGTCCAAT GGTGCTGGGT 
AACCTGTGGG ATGTGACAGA TCGTGACTTG GATCGATTCA CATCGGCTCT GCTCCAGTCT 
TGGCTCTCTG CTGGTTCTGG CTCTTCACTT CTTCAGCATT TGGCGAAATC ACGCAGCGCA 
ACTCATTTAA AACACATAAT CGGTGCTGCG CCCATTGCGT ATGGTTTACC GGTGCATATA 
CACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

espl1 ( XM_021474124.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021329799.1 Danio rerio extra spindle pole bodies like 1, separase (espl1), transcript variant X2, mRNA.

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CloneID	ODa146570
Clone ID Related Accession (Same CDS sequence)	XM_021474124.1
Accession Version	XM_021474124.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6543bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	separin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395261.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCATTAA AGAGCTTGAA AACAGAAGAT TACGTTCAGC AGTTATCCTG TGTCAAGGAC 
ACCATAGTCC TCCACAACGA ACTGAAGAAA CATGTGGATG GTGGCTTGGG TCCTTATGGT 
CGCACAATGT GTGATCGTAT CATCAGAGCC TGCAACCAGC GGCTGGGCAA CGGCTCTGTG 
GATGCTGGCC TTCAGGAGCG GATAGTGGAG CTGGTGGAAC TGGCTGTCCA AGGGTTTCAA 
CTGCTTGAAG AGTCCGGCAT GCAGAGCACC CCCTTTTATC TGGAGAAAAT AGTCTTTCAC 
ATCCTACAGA AAGTCGCAAG TCTTGGAGCT CATGGTCCTG CTTGTCGACT GGGTCAGCTC 
GTGTATAGAA GACTGAAATG TTTGTCTGCA GAGATTGACG ACTTTAATGT CCTGGTGCGG 
AATTGCTTTG CAGTCTTGTG GAACAGTCTC CTCTCTAACA AACCGAGTGC TTCCACCCTC 
CCTCCACGAG ACAGACTCCA CTGTCAGCTG CAAGCTGTAA TATTCAGACT CCTTGAACAG 
GGCTCCAGCT TGTCCTCTTC CCCATCCAAA GTTCCTCTGT TTGTTGAGGA AGCAGTAGTG 
GAGTACGAAC GATCATGTGG AGGTTTTACT CAAGACGATG TGAGTTTTCT GACCTCTGAA 
TTGCGTGAGC TGTTCTCTGG TGCTTTATTC AATCCTCAGG ACTCTGCATC AACTTCTTCA 
CTGCTGGCTG TGTGGTGTGA GGTGGCATTT AAAGTGTCTA AACTGCTCTG CAAAAGTGGC 
TTTTCACCTG AGGCTGTTGA ACTCTTGCGA GGCACTCTGA AGGAGGTTAA TGGCCACGTT 
GGCCTGCGTT CGGCTCTGGG TCTGGCAGAC CGGGCAGTGC AGATGCAGTG TGTGTTAAGT 
TCTGGAGGTG AATGCAGTAA GAGCTTTACT GAATGTGCCC GTGCTCTCCG GATGTTGCCT 
TGTGCCATGT CCAGCTCAGA GTCTCATGCC CTGTTAGAGG CTTGTCAGCT GGTGGTTTGG 
ACGTTGGAAG CTAGTCAGTG TAAAGGAATG GATGTGACCA CTTTGCTGGC TTGTTTTTCC 
TTTTTGGAAG AATATCAGGA ATTTCTCCCT ACACAGCAAA AGAACTCGTT TTCGCTGCAG 
GTGCAATACT CTCTTTGTTT CAGTTTTTAC CAGGGCTTCA TCTGCACGTA TGATAGTCTT 
CATGCATCTC AGGTGTCTGT GGGCGAGACC TTGGACAGAG TGCTATTGTT CTGCCGGTCG 
ACCGCAGGAC GTATGCTTGC TGAACTGCGG ACACTCAATA ATGACAACTT GCTGCTTAAA 
GCAGTGTGTG CAGTGAATAA TGTGGTGTAC GAGCTGTTTA ACAGGAAGCT GTATGAAGGA 
GCCTTTGGAT TGTCGATGAT TGTGTGTCAG GAGCTCTGTA AGGACTGCCC GCCCTCTCTT 
CCTGTGGATC GGGTCAACCG CTGTTTCATG CTGACAGTGC AGTGTAGTCG CCGCGCAGGC 
CATCTGGAGA AGGCTCTGGA TTGGATTGTT CGCTGGATTC AGGCTTTGGG CTCACAGATT 
TTGGATCATC TCACTGAACC TGTGTCGATG TGGGTCAAAA CTAAGTGTGA TGCCGCCAGA 
GCAGGCGAGG ATGACACACG CCTGAGGACT CTGAGGGACG GGTTAGGAGA ATCCTTAGTG 
GATGAGGAGG TTATGGTGTG TTTGTTAGAG GAGGAGTTGC GTGTATATAA GGAGCAGACT 
GGAGACACAG CACAGGAACG CTACAACACA CTCTGCGACC TCTTGGAAAT CTGCCATGAA 
GAAACCCCAC GCACACTAAG AAGAGCTACG TATCTGTGTG AGATGGCGCA GGTTGTGTGC 
TATCAGGACT TCAGCCAGCA GACTGACTGC TCAGCAGTTG ATTTTACCCA CGAGGCTTTG 
CGGTTGCTGG AAGCAGAACC AGAGACGGCA GAGAATACTG ATAGGCTGAA GGATGAGAAA 
GCCCACGCTT CCCTGTGGCT CTATATCTGC ACTCTTGAGT CTAACCTGCA GGAGGCTGTG 
GACACAGAGA AGCGTTTGTG TGCAGTGCAG GAGGAGAATA AAAGAGATGT GAATATGGAT 
CCTGTTCCCA CAAATGATCT GGAGTACGAG GACAAACAGA AATCACAAGA GAGCCAGTTA 
GTCTACGATG GACTACGCTT CAACCTGCTG GCTCACAGCA AGCAAAGTGA ACCTCTAGAT 
AGATGTTTGT CTCTATGGCG GAGTCTCCTC AGAGGGTCTG TGCCTGCAGT TAGAGACCCT 
AAACTCACAG CTTCTTCCAT GACTTTGATG GCTGCTCTAT ACACACTTAT GGGCAAACAC 
CTGCAAGCTC TGGAGGGATA TCAGCTTGCT GCCACCCTTT TCCATGTACT TGGTGATGGT 
CAGAATAGTG CCAATGCCTT CTGCCACTCT ACCAGGATCT TGCTTTACCT GGGTTCGCCG 
CAGCTTGCAC AGGTGGACTT AGAAAAAGCA GAAAAGAGCT TGTCATCCGT CACCATCTCT 
GAGGGCACAT CTATTATCTC CATGCTGGCC ATGTTGCAAA AAGCACAGCT ACACTATGCT 
CTAGGACAGG TGGAGAGTGG CGTGTCCTGC CTGATGGAGG TGATAAAGGA ATCTGCTCAG 
CATCATTCTA AGAGCTGGTA CCTCCTCAGG GCTCGAGCAC TGCAGACAGC CAGTGAATAC 
TTGAGTCTGG ACACACTGGC TTTGAACTCA CAGCTACGCC AGATCATCAT GCAGCAGGGT 
CTAAAGACTC CAGACACTGC TCAATATGAA GGGTTGAAGC TGTTGTGTAG CCTGGTGATG 
ATGTTATTGG GCAACGGGTT TTACGGAGCG CCTGGACCAA ACACTGACAC TCGCTTTGTG 
GATAAAGGAG ACAGTGTTGT GTTTAAGTGG TTGTTGCTGA GTGAAGTGCT GGTGTGCTCG 
GAGAGAATGG TGATCACCAG GAGCAGCAGC GGAGCCGTTC ATGAAGCTAA AGCTCAGTGT 
CTAGAGGCTC TCAAACTGGC CACCAAACTG CAGACACTCA GCCACTGTGC TGAGCTGTTG 
GTGCTGAAGG CAGAGCTGGA GCTGATGAAG GGTGCTACTG AAGCTAGTAG TTTAGACCTG 
GAGCAAGTTA GACATTTACT TGATCTCTGC ACAGATTTTG GCCAGGGACG ACAACAGAAA 
TCTGAAGTGA AAATCAAACC ACGCAAAGGT CGTCCCGCTG CCCCTTCTTC CTCTGGTCAC 
ATCTCTGAGG AAGAGGATGA TCTGAGTGGG ATCCTCAGCT CTCGTGCCCT TCCGAAGGAG 
CCTGTGGAGA ACATGGCTAG ATTAGGAAGC CAAGGAGCTT CTCCCCCTCT AAAGCCCAAA 
CGCCAGCATG GCCTCTCCTG TCTGACCCAT GAAGAGACCT GCTCTTGTCT CTGCTGCACT 
GAGCTCAGCG TGGCTCGAGT CAGCATCCAC TGGGCACTAT TACAGGCAGA TCTCCAGACA 
GATCCCGAAC ACTCTCTCAG ACTACGCCAG TCTGCCAGGA AACGCTGCCG CTATGTTGTA 
ACTAAACTTC AGAGAAGTTT GGCAGCACTT GTACACTTCA AAAAATCAAC TAATGTCTGC 
TTGTCACTGC TTCAGGCTGA ACAGGGTAAA GTGCATCTGG GTGCTGTGCT TCAACTGCTC 
AGAACTGGAG ACAAAGGGAA AGCTGCCATC TTGTGGGAGG AGATAGAAGC AGGATTAGAG 
GCAGTGACAC CTAAAGGAGC ACTGACACCA GAACTTGGGC CTATCAGAGC TGCTCTACTG 
GGGGCTAAAG CCGTAGCCTG CTGCTTGGCG TTGGCCATGA AGAAACAGTG TATACCCGAA 
GAGCTGTTTT CTTCATTGTG GCACTGGAAT GCAACAAAGG TTAAACCGCA ATTAAAATCT 
AAGCATCGAT CCAAGAGTCC GGACGCAGCT CCTCTAGAGC CAGGTGTTAA ACATAACCCT 
AAAGTTAAGA GTGGACTTGA GGAGAATGAA AGCAGAAAAA AAGAGACCAC TGTCATTTCG 
AAGAAAGCTA AAGACTCTGT GCCCAAGATT ACTATTACAA AATCCTCCAT GGTTTTTAAA 
ACGCCCAAAG CAACGAGAAC AACCCGGCCC AAATCTGTCT CCACTAGCAC TGGCATCAGT 
GACCTGAGAG CATTTGACTT CAATAATGAG GTGCCTGACA TTTCAGTCAA CTTCACACCA 
TCATTACACC GATCTACAAA CCAAAGTGCG ACTGCAAAGT CAAAAGCGTC CTCAAAGGGG 
TCGTTCCAGG TTTACAAAGA TTCTTCTCCT GCTGCAGAGA AAGCTGTAGT TGTCCCGGCT 
GCTCCTAAAC GAACAAAGCG TTCTCGCTTC AAGGTTGAGT TCAGCGATGA AAGCGACACC 
GAAGCTGCTC CTCTTGCTGT AATGGAAAAG TCAGAGAAAA AGAAAAACAC CTCTAAAACA 
TCACGCACCT TGAAACCAAC CCTTAACACC ATCCCTAGTG ACACCAAACT TTTGAGCTCC 
ACAGTAGATC CAGAACCCTC CAGACGAACA CGGACCACTA AGAAAAGCAC AGCTCTTCCC 
TCCATTGGTG GCCTCTCTTC TGAGGAAGAA CTTGGAGTAT CTTCACAGAC ACGTACGCGT 
AGAGGACGAC CAAAGAAGAG CACCAGCTCA GCAGACACAG AGGAGCCAGA GAGGATGAGG 
ATGATTAAGG AGGAAGAACA GAGGGTTCTT TTGAATGTCA GCATGGAGGA GCTCAGAGGC 
TCTGACACCG AGACAAAAGA CACTGGAAGT CCAGATGCTG ACTGTGAGGT CTTGAGGAGA 
GATCTAGGTG CAGATTTTGG GCGAGAGTGT TTCAGTGAGC TCAGGAGAGC TGGACAGAAT 
GGATCAGGAT CTCACACAGC TCTGCCACAT GCAACTACAT CTCCTGACTT GTCAGTAGAG 
ACGGTCCAGT CATACCTGCG CTCCTCATGG CTCCTCCTGC ACCACTTCCC GTTTCCTTCT 
CTTTATCCTC ACATCTGCTC ATTACTCGCT CAGTCATTCG GTCAAACGGA CCCCATCATC 
ACAGCAATGC TGCATGCACA GGCACTGGGA GTGTCCACTC GCCACCATAT GACCCGTCAC 
GTGGTTAACC AGTTCAGAAA GTTGAAGAAA TCCTGTAACG ATGTAGCAGA AGGTCTTGGA 
GCTCTGAGTC TGGAGGAAAC ATCTGGAGCA ACGCAGTCAA AGAAACTCTC TGCGTTGGAG 
CAGATCTTTT CATTCACAGC ATCAAACCCA ATGCAGTTTC CACAGACACA CTGTCAGCAG 
TTCACACAGC AGCTGGAAGA TCTACCTGCA GGAGTGACTG TATGCTTGCT GTCACTGACG 
GGGGTGCATC CTGATGAAAT TGGCAACACT ATTCTTCTGA CCCGACTGGA GCGCGGCTCT 
ACCCCAATCA CAGTTCGAAT CCCCACTGCA GACAGAGAGC AATCTGTTGC TGGCCTGTTG 
GAAGAAATGG ATGGAGTCTT GAAAGGACAA AAGGAAGTGA GCACAGTGGC TGAGAAATCA 
CAGTGGTGGG ACGGAAGGAA GGCTTTAGAT GCCCGTGTGG AGAAAATGCT GGAGGAGATG 
GAAGAGGCTT TAGGAGTGTG GCGAACTCTT CTTCTGCCTT TGACCTCTGA TCCTAAGCTT 
GATGTACAGG TAAAGAGCCT CCAAAAGGCT CTTAAATCAA CAAAGATCAC ACAGGATACT 
CTCAAGGTGA TTCTTTCAGC CTCTCCACTG CTGTCCCTGC CAGACCTGCA GTGTCTTCTG 
GAAGGAATGA ATGTGCAGGA CAAGGACCTG CTGAAGCTCC TTCAGAGAGG CGTGGCTGAA 
CTGAGAGGGA GGGAGGAGCC ACAGGGACAC ACTGTTCTGA TTTTAGACAA GTACTTGCAG 
AGGTTGCCGT GGGAGAACAT TGCTTGTCTG AAGTCTCAGT CGGTCACTCG AATGCCCTCA 
TTACATGCGG TGCTTGGACA CAGTCATCTG AGAGAGGTGG ATACAAGTTG TGTTCTGTCT 
TGTGGTGTGA ACCCTAAGCA GGTTTACTAT GTGCTAAACC CAGATGGAAA CCTGCCCGAC 
ACTGAGAATC GCTTCAAAGA GTGGTTTACA GGTGAACGAA CATGGCAGGG GGTTTGTGGA 
AGGGCTCCTG ATCCGGAGAA ACTACAAGAG GCTGTGACCA CTAAAGATCT CTACATATAC 
ATCGGACATG GTGCAGGAGC CCGCTTTCTG GATGCGCAGA GAGTTTTGAA GGTGCCTGTG 
AGGGCCGCAG CTCTTCTGTT CGGCTGCAGT AGTGCTGCTC TCAGTGTGCT TGGTCAGCAG 
GAGGGAACGG GCATCATCCT CAGCTACCTG ACCGCTGGAT GTCCAATGGT GCTGGGTAAC 
CTGTGGGATG TGACAGATCG TGACTTGGAT CGATTCACAT CGGCTCTGCT CCAGTCTTGG 
CTCTCTGCTG GTTCTGGCTC TTCACTTCTT CAGCATTTGG CGAAATCACG CAGCGCAACT 
CATTTAAAAC ACATAATCGG TGCTGCGCCC ATTGCGTATG GTTTACCGGT GCATATACAC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021329799.1
CDS	222..6764
Translation	MALKSLKTEDYVQQLSCVKDTIVLHNELKKHVDGGLGPYGRTMCDRIIRACNQRLGNGSVDAGLQERIVELVELAVQGFQLLEESGMQSTPFYLEKIVFHILQKVASLGAHGPACRLGQLVYRRLKCLSAEIDDFNVLVRNCFAVLWNSLLSNKPSASTLPPRDRLHCQLQAVIFRLLEQGSSLSSSPSKVPLFVEEAVVEYERSCGGFTQDDVSFLTSELRELFSGALFNPQDSASTSSLLAVWCEVAFKVSKLLCKSGFSPEAVELLRGTLKEVNGHVGLRSALGLADRAVQMQCVLSSGGECSKSFTECARALRMLPCAMSSSESHALLEACQLVVWTLEASQCKGMDVTTLLACFSFLEEYQEFLPTQQKNSFSLQVQYSLCFSFYQGFICTYDSLHASQVSVGETLDRVLLFCRSTAGRMLAELRTLNNDNLLLKAVCAVNNVVYELFNRKLYEGAFGLSMIVCQELCKDCPPSLPVDRVNRCFMLTVQCSRRAGHLEKALDWIVRWIQALGSQILDHLTEPVSMWVKTKCDAARAGEDDTRLRTLRDGLGESLVDEEVMVCLLEEELRVYKEQTGDTAQERYNTLCDLLEICHEETPRTLRRATYLCEMAQVVCYQDFSQQTDCSAVDFTHEALRLLEAEPETAENTDRLKDEKAHASLWLYICTLESNLQEAVDTEKRLCAVQEENKRDVNMDPVPTNDLEYEDKQKSQESQLVYDGLRFNLLAHSKQSEPLDRCLSLWRSLLRGSVPAVRDPKLTASSMTLMAALYTLMGKHLQALEGYQLAATLFHVLGDGQNSANAFCHSTRILLYLGSPQLAQVDLEKAEKSLSSVTISEGTSIISMLAMLQKAQLHYALGQVESGVSCLMEVIKESAQHHSKSWYLLRARALQTASEYLSLDTLALNSQLRQIIMQQGLKTPDTAQYEGLKLLCSLVMMLLGNGFYGAPGPNTDTRFVDKGDSVVFKWLLLSEVLVCSERMVITRSSSGAVHEAKAQCLEALKLATKLQTLSHCAELLVLKAELELMKGATEASSLDLEQVRHLLDLCTDFGQGRQQKSEVKIKPRKGRPAAPSSSGHISEEEDDLSGILSSRALPKEPVENMARLGSQGASPPLKPKRQHGLSCLTHEETCSCLCCTELSVARVSIHWALLQADLQTDPEHSLRLRQSARKRCRYVVTKLQRSLAALVHFKKSTNVCLSLLQAEQGKVHLGAVLQLLRTGDKGKAAILWEEIEAGLEAVTPKGALTPELGPIRAALLGAKAVACCLALAMKKQCIPEELFSSLWHWNATKVKPQLKSKHRSKSPDAAPLEPGVKHNPKVKSGLEENESRKKETTVISKKAKDSVPKITITKSSMVFKTPKATRTTRPKSVSTSTGISDLRAFDFNNEVPDISVNFTPSLHRSTNQSATAKSKASSKGSFQVYKDSSPAAEKAVVVPAAPKRTKRSRFKVEFSDESDTEAAPLAVMEKSEKKKNTSKTSRTLKPTLNTIPSDTKLLSSTVDPEPSRRTRTTKKSTALPSIGGLSSEEELGVSSQTRTRRGRPKKSTSSADTEEPERMRMIKEEEQRVLLNVSMEELRGSDTETKDTGSPDADCEVLRRDLGADFGRECFSELRRAGQNGSGSHTALPHATTSPDLSVETVQSYLRSSWLLLHHFPFPSLYPHICSLLAQSFGQTDPIITAMLHAQALGVSTRHHMTRHVVNQFRKLKKSCNDVAEGLGALSLEETSGATQSKKLSALEQIFSFTASNPMQFPQTHCQQFTQQLEDLPAGVTVCLLSLTGVHPDEIGNTILLTRLERGSTPITVRIPTADREQSVAGLLEEMDGVLKGQKEVSTVAEKSQWWDGRKALDARVEKMLEEMEEALGVWRTLLLPLTSDPKLDVQVKSLQKALKSTKITQDTLKVILSASPLLSLPDLQCLLEGMNVQDKDLLKLLQRGVAELRGREEPQGHTVLILDKYLQRLPWENIACLKSQSVTRMPSLHAVLGHSHLREVDTSCVLSCGVNPKQVYYVLNPDGNLPDTENRFKEWFTGERTWQGVCGRAPDPEKLQEAVTTKDLYIYIGHGAGARFLDAQRVLKVPVRAAALLFGCSSAALSVLGQQEGTGIILSYLTAGCPMVLGNLWDVTDRDLDRFTSALLQSWLSAGSGSSLLQHLAKSRSATHLKHIIGAAPIAYGLPVHIH

Target ORF information:

RefSeq Version	XM_021474124.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio extra spindle pole bodies like 1, separase (espl1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021474124.1

ORF Insert Sequence:

ATGGCATTAA AGAGCTTGAA AACAGAAGAT TACGTTCAGC AGTTATCCTG TGTCAAGGAC 
ACCATAGTCC TCCACAACGA ACTGAAGAAA CATGTGGATG GTGGCTTGGG TCCTTATGGT 
CGCACAATGT GTGATCGTAT CATCAGAGCC TGCAACCAGC GGCTGGGCAA CGGCTCTGTG 
GATGCTGGCC TTCAGGAGCG GATAGTGGAG CTGGTGGAAC TGGCTGTCCA AGGGTTTCAA 
CTGCTTGAAG AGTCCGGCAT GCAGAGCACC CCCTTTTATC TGGAGAAAAT AGTCTTTCAC 
ATCCTACAGA AAGTCGCAAG TCTTGGAGCT CATGGTCCTG CTTGTCGACT GGGTCAGCTC 
GTGTATAGAA GACTGAAATG TTTGTCTGCA GAGATTGACG ACTTTAATGT CCTGGTGCGG 
AATTGCTTTG CAGTCTTGTG GAACAGTCTC CTCTCTAACA AACCGAGTGC TTCCACCCTC 
CCTCCACGAG ACAGACTCCA CTGTCAGCTG CAAGCTGTAA TATTCAGACT CCTTGAACAG 
GGCTCCAGCT TGTCCTCTTC CCCATCCAAA GTTCCTCTGT TTGTTGAGGA AGCAGTAGTG 
GAGTACGAAC GATCATGTGG AGGTTTTACT CAAGACGATG TGAGTTTTCT GACCTCTGAA 
TTGCGTGAGC TGTTCTCTGG TGCTTTATTC AATCCTCAGG ACTCTGCATC AACTTCTTCA 
CTGCTGGCTG TGTGGTGTGA GGTGGCATTT AAAGTGTCTA AACTGCTCTG CAAAAGTGGC 
TTTTCACCTG AGGCTGTTGA ACTCTTGCGA GGCACTCTGA AGGAGGTTAA TGGCCACGTT 
GGCCTGCGTT CGGCTCTGGG TCTGGCAGAC CGGGCAGTGC AGATGCAGTG TGTGTTAAGT 
TCTGGAGGTG AATGCAGTAA GAGCTTTACT GAATGTGCCC GTGCTCTCCG GATGTTGCCT 
TGTGCCATGT CCAGCTCAGA GTCTCATGCC CTGTTAGAGG CTTGTCAGCT GGTGGTTTGG 
ACGTTGGAAG CTAGTCAGTG TAAAGGAATG GATGTGACCA CTTTGCTGGC TTGTTTTTCC 
TTTTTGGAAG AATATCAGGA ATTTCTCCCT ACACAGCAAA AGAACTCGTT TTCGCTGCAG 
GTGCAATACT CTCTTTGTTT CAGTTTTTAC CAGGGCTTCA TCTGCACGTA TGATAGTCTT 
CATGCATCTC AGGTGTCTGT GGGCGAGACC TTGGACAGAG TGCTATTGTT CTGCCGGTCG 
ACCGCAGGAC GTATGCTTGC TGAACTGCGG ACACTCAATA ATGACAACTT GCTGCTTAAA 
GCAGTGTGTG CAGTGAATAA TGTGGTGTAC GAGCTGTTTA ACAGGAAGCT GTATGAAGGA 
GCCTTTGGAT TGTCGATGAT TGTGTGTCAG GAGCTCTGTA AGGACTGCCC GCCCTCTCTT 
CCTGTGGATC GGGTCAACCG CTGTTTCATG CTGACAGTGC AGTGTAGTCG CCGCGCAGGC 
CATCTGGAGA AGGCTCTGGA TTGGATTGTT CGCTGGATTC AGGCTTTGGG CTCACAGATT 
TTGGATCATC TCACTGAACC TGTGTCGATG TGGGTCAAAA CTAAGTGTGA TGCCGCCAGA 
GCAGGCGAGG ATGACACACG CCTGAGGACT CTGAGGGACG GGTTAGGAGA ATCCTTAGTG 
GATGAGGAGG TTATGGTGTG TTTGTTAGAG GAGGAGTTGC GTGTATATAA GGAGCAGACT 
GGAGACACAG CACAGGAACG CTACAACACA CTCTGCGACC TCTTGGAAAT CTGCCATGAA 
GAAACCCCAC GCACACTAAG AAGAGCTACG TATCTGTGTG AGATGGCGCA GGTTGTGTGC 
TATCAGGACT TCAGCCAGCA GACTGACTGC TCAGCAGTTG ATTTTACCCA CGAGGCTTTG 
CGGTTGCTGG AAGCAGAACC AGAGACGGCA GAGAATACTG ATAGGCTGAA GGATGAGAAA 
GCCCACGCTT CCCTGTGGCT CTATATCTGC ACTCTTGAGT CTAACCTGCA GGAGGCTGTG 
GACACAGAGA AGCGTTTGTG TGCAGTGCAG GAGGAGAATA AAAGAGATGT GAATATGGAT 
CCTGTTCCCA CAAATGATCT GGAGTACGAG GACAAACAGA AATCACAAGA GAGCCAGTTA 
GTCTACGATG GACTACGCTT CAACCTGCTG GCTCACAGCA AGCAAAGTGA ACCTCTAGAT 
AGATGTTTGT CTCTATGGCG GAGTCTCCTC AGAGGGTCTG TGCCTGCAGT TAGAGACCCT 
AAACTCACAG CTTCTTCCAT GACTTTGATG GCTGCTCTAT ACACACTTAT GGGCAAACAC 
CTGCAAGCTC TGGAGGGATA TCAGCTTGCT GCCACCCTTT TCCATGTACT TGGTGATGGT 
CAGAATAGTG CCAATGCCTT CTGCCACTCT ACCAGGATCT TGCTTTACCT GGGTTCGCCG 
CAGCTTGCAC AGGTGGACTT AGAAAAAGCA GAAAAGAGCT TGTCATCCGT CACCATCTCT 
GAGGGCACAT CTATTATCTC CATGCTGGCC ATGTTGCAAA AAGCACAGCT ACACTATGCT 
CTAGGACAGG TGGAGAGTGG CGTGTCCTGC CTGATGGAGG TGATAAAGGA ATCTGCTCAG 
CATCATTCTA AGAGCTGGTA CCTCCTCAGG GCTCGAGCAC TGCAGACAGC CAGTGAATAC 
TTGAGTCTGG ACACACTGGC TTTGAACTCA CAGCTACGCC AGATCATCAT GCAGCAGGGT 
CTAAAGACTC CAGACACTGC TCAATATGAA GGGTTGAAGC TGTTGTGTAG CCTGGTGATG 
ATGTTATTGG GCAACGGGTT TTACGGAGCG CCTGGACCAA ACACTGACAC TCGCTTTGTG 
GATAAAGGAG ACAGTGTTGT GTTTAAGTGG TTGTTGCTGA GTGAAGTGCT GGTGTGCTCG 
GAGAGAATGG TGATCACCAG GAGCAGCAGC GGAGCCGTTC ATGAAGCTAA AGCTCAGTGT 
CTAGAGGCTC TCAAACTGGC CACCAAACTG CAGACACTCA GCCACTGTGC TGAGCTGTTG 
GTGCTGAAGG CAGAGCTGGA GCTGATGAAG GGTGCTACTG AAGCTAGTAG TTTAGACCTG 
GAGCAAGTTA GACATTTACT TGATCTCTGC ACAGATTTTG GCCAGGGACG ACAACAGAAA 
TCTGAAGTGA AAATCAAACC ACGCAAAGGT CGTCCCGCTG CCCCTTCTTC CTCTGGTCAC 
ATCTCTGAGG AAGAGGATGA TCTGAGTGGG ATCCTCAGCT CTCGTGCCCT TCCGAAGGAG 
CCTGTGGAGA ACATGGCTAG ATTAGGAAGC CAAGGAGCTT CTCCCCCTCT AAAGCCCAAA 
CGCCAGCATG GCCTCTCCTG TCTGACCCAT GAAGAGACCT GCTCTTGTCT CTGCTGCACT 
GAGCTCAGCG TGGCTCGAGT CAGCATCCAC TGGGCACTAT TACAGGCAGA TCTCCAGACA 
GATCCCGAAC ACTCTCTCAG ACTACGCCAG TCTGCCAGGA AACGCTGCCG CTATGTTGTA 
ACTAAACTTC AGAGAAGTTT GGCAGCACTT GTACACTTCA AAAAATCAAC TAATGTCTGC 
TTGTCACTGC TTCAGGCTGA ACAGGGTAAA GTGCATCTGG GTGCTGTGCT TCAACTGCTC 
AGAACTGGAG ACAAAGGGAA AGCTGCCATC TTGTGGGAGG AGATAGAAGC AGGATTAGAG 
GCAGTGACAC CTAAAGGAGC ACTGACACCA GAACTTGGGC CTATCAGAGC TGCTCTACTG 
GGGGCTAAAG CCGTAGCCTG CTGCTTGGCG TTGGCCATGA AGAAACAGTG TATACCCGAA 
GAGCTGTTTT CTTCATTGTG GCACTGGAAT GCAACAAAGG TTAAACCGCA ATTAAAATCT 
AAGCATCGAT CCAAGAGTCC GGACGCAGCT CCTCTAGAGC CAGGTGTTAA ACATAACCCT 
AAAGTTAAGA GTGGACTTGA GGAGAATGAA AGCAGAAAAA AAGAGACCAC TGTCATTTCG 
AAGAAAGCTA AAGACTCTGT GCCCAAGATT ACTATTACAA AATCCTCCAT GGTTTTTAAA 
ACGCCCAAAG CAACGAGAAC AACCCGGCCC AAATCTGTCT CCACTAGCAC TGGCATCAGT 
GACCTGAGAG CATTTGACTT CAATAATGAG GTGCCTGACA TTTCAGTCAA CTTCACACCA 
TCATTACACC GATCTACAAA CCAAAGTGCG ACTGCAAAGT CAAAAGCGTC CTCAAAGGGG 
TCGTTCCAGG TTTACAAAGA TTCTTCTCCT GCTGCAGAGA AAGCTGTAGT TGTCCCGGCT 
GCTCCTAAAC GAACAAAGCG TTCTCGCTTC AAGGTTGAGT TCAGCGATGA AAGCGACACC 
GAAGCTGCTC CTCTTGCTGT AATGGAAAAG TCAGAGAAAA AGAAAAACAC CTCTAAAACA 
TCACGCACCT TGAAACCAAC CCTTAACACC ATCCCTAGTG ACACCAAACT TTTGAGCTCC 
ACAGTAGATC CAGAACCCTC CAGACGAACA CGGACCACTA AGAAAAGCAC AGCTCTTCCC 
TCCATTGGTG GCCTCTCTTC TGAGGAAGAA CTTGGAGTAT CTTCACAGAC ACGTACGCGT 
AGAGGACGAC CAAAGAAGAG CACCAGCTCA GCAGACACAG AGGAGCCAGA GAGGATGAGG 
ATGATTAAGG AGGAAGAACA GAGGGTTCTT TTGAATGTCA GCATGGAGGA GCTCAGAGGC 
TCTGACACCG AGACAAAAGA CACTGGAAGT CCAGATGCTG ACTGTGAGGT CTTGAGGAGA 
GATCTAGGTG CAGATTTTGG GCGAGAGTGT TTCAGTGAGC TCAGGAGAGC TGGACAGAAT 
GGATCAGGAT CTCACACAGC TCTGCCACAT GCAACTACAT CTCCTGACTT GTCAGTAGAG 
ACGGTCCAGT CATACCTGCG CTCCTCATGG CTCCTCCTGC ACCACTTCCC GTTTCCTTCT 
CTTTATCCTC ACATCTGCTC ATTACTCGCT CAGTCATTCG GTCAAACGGA CCCCATCATC 
ACAGCAATGC TGCATGCACA GGCACTGGGA GTGTCCACTC GCCACCATAT GACCCGTCAC 
GTGGTTAACC AGTTCAGAAA GTTGAAGAAA TCCTGTAACG ATGTAGCAGA AGGTCTTGGA 
GCTCTGAGTC TGGAGGAAAC ATCTGGAGCA ACGCAGTCAA AGAAACTCTC TGCGTTGGAG 
CAGATCTTTT CATTCACAGC ATCAAACCCA ATGCAGTTTC CACAGACACA CTGTCAGCAG 
TTCACACAGC AGCTGGAAGA TCTACCTGCA GGAGTGACTG TATGCTTGCT GTCACTGACG 
GGGGTGCATC CTGATGAAAT TGGCAACACT ATTCTTCTGA CCCGACTGGA GCGCGGCTCT 
ACCCCAATCA CAGTTCGAAT CCCCACTGCA GACAGAGAGC AATCTGTTGC TGGCCTGTTG 
GAAGAAATGG ATGGAGTCTT GAAAGGACAA AAGGAAGTGA GCACAGTGGC TGAGAAATCA 
CAGTGGTGGG ACGGAAGGAA GGCTTTAGAT GCCCGTGTGG AGAAAATGCT GGAGGAGATG 
GAAGAGGCTT TAGGAGTGTG GCGAACTCTT CTTCTGCCTT TGACCTCTGA TCCTAAGCTT 
GATGTACAGG TAAAGAGCCT CCAAAAGGCT CTTAAATCAA CAAAGATCAC ACAGGATACT 
CTCAAGGTGA TTCTTTCAGC CTCTCCACTG CTGTCCCTGC CAGACCTGCA GTGTCTTCTG 
GAAGGAATGA ATGTGCAGGA CAAGGACCTG CTGAAGCTCC TTCAGAGAGG CGTGGCTGAA 
CTGAGAGGGA GGGAGGAGCC ACAGGGACAC ACTGTTCTGA TTTTAGACAA GTACTTGCAG 
AGGTTGCCGT GGGAGAACAT TGCTTGTCTG AAGTCTCAGT CGGTCACTCG AATGCCCTCA 
TTACATGCGG TGCTTGGACA CAGTCATCTG AGAGAGGTGG ATACAAGTTG TGTTCTGTCT 
TGTGGTGTGA ACCCTAAGCA GGTTTACTAT GTGCTAAACC CAGATGGAAA CCTGCCCGAC 
ACTGAGAATC GCTTCAAAGA GTGGTTTACA GGTGAACGAA CATGGCAGGG GGTTTGTGGA 
AGGGCTCCTG ATCCGGAGAA ACTACAAGAG GCTGTGACCA CTAAAGATCT CTACATATAC 
ATCGGACATG GTGCAGGAGC CCGCTTTCTG GATGCGCAGA GAGTTTTGAA GGTGCCTGTG 
AGGGCCGCAG CTCTTCTGTT CGGCTGCAGT AGTGCTGCTC TCAGTGTGCT TGGTCAGCAG 
GAGGGAACGG GCATCATCCT CAGCTACCTG ACCGCTGGAT GTCCAATGGT GCTGGGTAAC 
CTGTGGGATG TGACAGATCG TGACTTGGAT CGATTCACAT CGGCTCTGCT CCAGTCTTGG 
CTCTCTGCTG GTTCTGGCTC TTCACTTCTT CAGCATTTGG CGAAATCACG CAGCGCAACT 
CATTTAAAAC ACATAATCGG TGCTGCGCCC ATTGCGTATG GTTTACCGGT GCATATACAC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

espl1 ( XM_021477220.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021332895.1 Danio rerio extra spindle pole bodies like 1, separase (espl1), transcript variant X1, mRNA.

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CloneID	ODa12112
Clone ID Related Accession (Same CDS sequence)	XM_021477220.1 , XM_001337833.4 , XM_001337833.5 , XM_017356662.1
Accession Version	XM_021477220.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6549bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	separin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCATTAA AGAGCTTGAA AACAGAAGAT TACGTTCAGC AGTTATCCTG TGTCAAGGAC 
ACCATAGTCC TCCACAACGA ACTGAAGAAA CATGTGGATG GTGGCTTGGG TCCTTATGGT 
CGCACAATGT GTGATCGTAT CATCAGAGCC TGCAACCAGC GGCTGGGCAA CGGCGCTGTG 
GATGCTGGCC TTCAGGAGCG GATAGTGGAG CTGGTGGAAC TGGCTGTCCA AGGGTTTCAA 
CTGCTTGAAG AGTCCGGCAT GCAGAGCACC CCCTTTTATC TGGAGAAAAT AGTCTTTCAC 
ATCCTACAGA AAGTCGCAAG CCTTGGAGCT CATGGTCCTG CTTGTCGACT GGGTCAGCTC 
GTGTATAGAA GACTGAAATG TTTGTCTGCA GAGATTGATG ACTTTAATGT CCTGGTGCGG 
AATTGCTTTG CAGTCTTGTG GAACAGTCTC CTCTCTAACA AACCGAGTGC TTCCACCCTC 
CCTCCACGAG ACAGACTCCA CTGTCAGCTG CAAGCTTTAA CATTCAGACT CCTTGAACAG 
GGCTCCAGCT CGTCCTCTTC CCCATCCAAA GTTCCTCTGT TCGTGGAGGA AGCAGTAGTG 
GAGTACGAAC GATCATGTGG AGGTTTTACT CAAGACGATG TGAGTTTTCT GACCTCTGAA 
TTGCGTGAGC TGTTCTCTGG TGCTTTCATC AATCCTCAGG ACTCTGCATC AACTTCTTCA 
CTGCTGGCTG TGTGGTGTGA GGTGGCATTT AAAGTGTCTA AACTGCTCTG CAAAAGTAGC 
TTTTCACCTG AGGCTGTTGA ACTCTTGCGA GGCACTCTGA AGGAGGTTAA TGGCCACGTT 
GGCCTGCGTT CGGCTCTGGG TCTGGCAGAC CGGGCAGTGC AGATGCAGTG TGTGTTAAGT 
TCTGGAGGTG AATGCAGTAA GAGCTTCACT GAATGTGCCC GTGCTCTCCG GATGTTGCCT 
TGTGCCATGT CCAGCTCAGA GTCTCATGCC CTGTTAGAGG CTTGTCAGCT GGTGGTTTGG 
ACGTTGGAAG CCAGTCAGTG TAAAGGAATG GATGTGACCA CTTTGCTGGC TTGTTTTTCC 
TTTTTGGAAG AATATCAGGA ATTTCTGCTT GCACAGCAAA AGAACTCGTT TTCGCTGCAG 
GTCCAATACT CTCTTTGTTT CAGTTTTTAC CAGGGCTTCA TCTGCACATA TGATAGTCTT 
CATGCTTCTC AGGTGTCTGT GGGCGAGACC TTGGACAGAG TGCTATTGTT CTGCCGGTCG 
ACCGCAGGAC GTATGCTTGC TGAACTGCGG ACACTCAATA ATGACAACTT GCTGCTTAAA 
GCAGTGTGTG CAGTGAATAA TGTGGTGTAC GAGCTGTTTA ACAGGAAGCT GTATGAAGGA 
GCCTTTGGAT TGTCGATGAT TGTGTGTCAG GAGCTCTGTA AGGACTGCCC GCCCTCTCTT 
CCTATGGATC GGGTCAACCG CTGTTTCATG CTGACAGTGC AGTGTAGTCG CCGCGCAGGC 
CATCTGGAGC AGGCTCTGGA CTGGATTGTT CGCTGGATTC AGGCTTTGGG CTCACAGATT 
TTGGATCATC TCACTGAACC TGTGTCGATG TGGGTCAAAA CTAAGTGTGA TGCCGCCAGA 
GCAGGCGAGG ATGACACACG CCTGAGGACT CTGAGGGACG GGTTAGGAGA ATCCTTAGTG 
GATGAGGAGG TTATGATGTG TTTGTTAGAG GAGGAGTTGC GTGTATATAA GGAGCAGACT 
GGAGACACAG CACAGGAACG CTACAACACA CTCTGCGACC TCTTGGAAAT CTGCCATGAA 
GAAACCCCAC ACACACTAAG AAGAGCTACG TATCTGTGTG AGATGGCACA GGTTGTGTGC 
TATCAGGACT TCAGCCAGCA GACTGACTGC TCAGCAGTTG ATTTTACCCA CGAGGCTTTG 
CGGTTGCTGG AAGCAGAACC AGAGACGGCA GAGAATACTG ATAGGCTGAA GGATGAGAAA 
GCCCACGCTT CCCTGTGGCT CTATATCTGC ACTCTTGAGT CTAACCTGCA GGAGGCTGTG 
GACACAGAGA AGCGTTTGTG TGCAGTGCAG GAGGAGAATA AAAGAGATGT GAATATGGAT 
CCTGTTCCCA CAAATGATCT GGAGTACGAG GACAAACAGA AATCACAAGA GAGCCAGTTA 
GTCTACGATG GACTACGCTT CAACCTGCTG GCTCACAGCA AGCAAAGTGA ACCTCTAGAT 
AAATGTTTGT CTCTATGGCG GAGTCTCCTC AAGGGGGCTG TGCCTGCAGT TAGAGACCCT 
AAACTCACAG CTTCTTCCAT GACTTTGATG GCTGCCCTAT ACACACTTAT GGGCAAGCAC 
CTGCAAGCTC TGGAGGGATA TCAGCTTGCT GCCACCCTTT TCCATGTACT TGGTGATGGC 
CAGAGTAGTG CCAATGCCTT CTGCCACTCT ACCAGGATCT TGCTTTACCT GGGTTCACCG 
CAGCTTGCAC AGGTGGACTT AGAAAAAGCA GAAAAGAGCT TGTCATCTGT CACCATCTCT 
GAGGGCACAT CTATTATCTC CATGCTGGCC ATGTTGCAAA AAGCACAGCT ACACTATGCT 
CTAGGACAGG TGGAGAGTGG CGTGTCCTGC CTGATGGAGG TGATAAAGGA ATCTGCTCAG 
CATCATTCTA AGAGCTGGTA CCTGCTCAGG GCTCGAGCAC TGCAGACAGC CAGTGAATAC 
TTGAGTCTGG ACACACTGGC TTTGAACTCA CAGCTACGCC AGAGCATCAT GCAGCAGGGT 
CTAAAGACTC CAGACACTGC TCAATATGAA GGGTTGAAGC TGTTGTGTAG CCTGGTGATG 
ATGTTATTGG GCAACGGGTT TTACGGAGCG CCTGGACCAA ACACTGACAC TCGCTTTGTG 
GATAAAGGAG ACAGTGTTGT GTTTAAGTGG CTGCTGCTGA GTGAAGTGCT GGTGTGCTCG 
GAGAGAATGG TGATCACCAG GAGCAGCAGC GGAGCCGTTC ATGAAGCTAA AGCTCAGTGT 
CTAGAGGCTC TCAAACTGGC CACCAAACTA CAGACACTCA GCCACTGTGC TGAGCTGTTG 
GTGCTGAAGG CAGAGCTGGA GCTGATGAAG GGTGCAACTG AAGCTAGTAG TTTAGACCTA 
GAGCAAGTTA GACATTTACT TGATCTCTGC ACAGATTTTG GCCAGGGACG ACAACAGAAA 
TCTGAAGTGA AAATCAAACC ACGCAAAGGT CGTCCCGCTG CCCCTTCTTC CTCTGGTCAC 
ATCTCTGAGG AAGAGGATGA TCTGAGTGGG ATCCTCAGCT CTCGTGCCCT TCCGAAGGAG 
CCTGTGGAGA ACATGGCTAG ATTAGGAAGC CAAGGAGCTT CTCCCCCTCT AAAGCCCAAA 
TGCCAGCATG GCCTCTCCTG TCTGACCCAT GAAGAGACCT GCTCTTGTCT CTGCTGCACT 
GAGCTCAGCG TGGCTCGAGT CAGCATCCAC TGGGCACTAT TACAGGCAGA TCTCCAGACA 
GATCCCGAAC GCTCTCTCAG ACTACGCCAG TCTGCCAGGA AACGCTGCCG CTATGTTGTA 
ACTAAACTTC AGAGAAGTTT GGCAGCACTT GTACACTCCA AAAAATCACC TAATGTCTGC 
TTGTCACTGC TTCAGGCTGA ACAGGGTAAA GTGCATCTGG GTGCTGTGCT TCAACTGCTC 
AGAACTGGAG ACAAAGGGAA AGCTGCCATC TTGTGGGAGG AGATAGAAGC AGGATTAGAG 
GCAGTGACAC CTAAAGGAGC ACTGACACCA GAACTTGGGC CTATCAGAGC TACTCTACTG 
GGGGCTAAAG CCGTAGCCTG CTGCTTGGCG TTGGCAATGA AGAAACAGTG TATACCCGAA 
GAGCTGTTTT CTTCATTGTG GCACTGGAAT CCACCAAAGG TTAAACCGCA ATTAAAATCT 
AAGCATCGAT CCAAGAGTCC GGACGCAGCT CCTCTAGAGG CAGGTGTTAA ACATAACCCT 
AAAGTTAAGA GTGGACTTGA GGAGAATGAA AGCAGAAAAA CCAAAGAGAC CACTGTCATT 
TCGAAGAAAG CTAAAGACTC TGTGCCCAAA ATTACCATCA CAAAATCCTC CATGGTTTTT 
AAAACGCCCA AAGCAACGAG AACAACCCGG CCCAAATCTG TCTCCACTAG CACTGGCATC 
AGTGACCTGA GAGCATTTGA CTTCAATAAT GAGGTGCCTG ACATTTCAGT CAACTTCACA 
CCATCATTAC ACCGATCTAC AAACCAAAGT GCGACTGCAA AGTCAAAAGC GTCCTCAAAG 
GGTTCGTTCC AGGTTTACAA AGATTCTTCT CCTGCTGCAG AGAAAGCTGT AGTTGTCCCG 
GCTGCTCCTA AACGAACAAA GCGTTCTCGC TTCAAGGTTG AGTTCAGCGA TGAAAGCGAC 
AACGATGCTG CTCCTCTTGC TGTAATGGAA AAGTCAGAGA AAAAGAAAAA CACCTTTAAA 
ACATCACGCA CCTTGAAACC AACCCTTAAC ACCATCCCTA GTGACACCAA ACTTGTGAGC 
TCCACAGTAG ATCCAGAACC CTCCAGACGA ACACGGACCA CTAAGAAAAG CACAGCTCTT 
CCCTCCATTG GTGGCCTCTC TTCTGAGGAA GAACTTGGAG TATCTTCACA GACACGTACG 
CGTAGAGGAC GACCAAAGAA GAGCACCAGC TCAGCAGACA CAGAGGAGCC AGAGAGGATG 
AGGATGATTA AGGAGGAAGA ACAGAGGGTT CTTTTGAATG TCAGCATGGA GGAGCTCAGA 
GGCTCTGACA CTGAGACAAA AGACACTGGA AGTCCAGATG CTGACTGTGA GGTCTTGAGG 
AGAGATCTAG GTGCAGATTT TGGGCGAGAG TGTTTCAGTG AGCTCAGGAG AGCTGGACAG 
AATGGATCAG GATCTCACAC AGCTCTGCCA CATGCAACTA CATCTCCTGC AGACTTGTCA 
GTAGAGACGG TCCAGTCATA CCTGCGCTCC TCATGGCTCC TCCTGCACCA CTTCCCGTTT 
CCTTCTCTTT ATCCTCACAT CTGCTCATTA CTCGCTCAGT CATTCGGTCA AACGGACCCC 
ATCATCACAG CAATGCTGCA TGCACAGGCA CTGGGAGTGT CCACTCGCCA CCATATGACC 
CGTCACGTGG TTAACCAGTT CAGAAAGTTG AAGAAATCCT GTAACGATGT AGCAGAAGGT 
CTTGGAGCTC TGAGTCTGGA GGAAACATCT GGAGCAACGC AGTCAAAGAA ACTCTCTGCG 
TTGGAGCAGA TCTTTTCATT CACAGCATCA AACCCAATGC AGTTTCCACA GACACACTGT 
CAGCAGTTCA CACAGCAGCT GGAAGATCTA CCTGCAGGAG TGACCGTATG CTTGCTGTCA 
CTGACGGGGG TGCATCCTGA TGAAATTGGC AACACTATTC TTCTGACCCG ACTGGAGCGC 
GGCTCTACCC CAATCACAGT TCGAATCCCC ACTGCAGACA GAGAGCAATC TGTTGCTGGC 
CTGTTGGAAG AAATGGATGG AGTCTTGAAA GGACAAAAGG AAGTGAGCAC AGTGGCTGAG 
AAATCACAGT GGTGGGACGG AAGGAAGGCT TTAGATGCCC GTGTGGAGAA AATGCTGGAG 
GAGATGGAAG AGGCTTTAGG AGTGTGGCGA ACTCTTTTTC TGCCTTTGAC CTCTGATCCT 
AAGCTTGATG TTCAGGTAAA GAGCCTCCAA AAGGCTCTTA AATCAACAAA GATCACACGG 
GACACTCTCA AGGTGATTCT ATCAGCGTCT CCACTGCTGT CCCTGCCAGA CCTGCAGTGT 
CTTCTGGAAG GAATGAATGT GCAGGACAAG GACCTGCTGA AGCTCCTGCA GAGAGGCGTG 
GCTGAACTGA GAGGGAGGGA GGAGCCACAG GGACACACTG TTCTGATTTT AGACAAGTAC 
TTGCAGAGGT TGCCGTGGGA GAACATTGCT TGTCTGAAGT CTCAGTCGGT CACTCGAATG 
CCCTCATTAC ACGCGGTGCT TGGACACAGT CATCTGAGAG AGGTGGATAC AAGTTGTGTT 
CTATCTTGTG GTGTGAACCC TAAGCAGGTT TACTATGTGC TAAACCCAGA TGGAAACCTG 
CCCGACACTG AGAATCGCTT CAAAGAGTGG TTTACAGGTG AACGAACATG GCAGGGGGTT 
TGTGGAAGGG CTCCTGATCC GGAGAAACTA CAAGAGGCTG TGACCACTAA AGATCTCTAC 
ATATACATCG GACATGGTGC AGGAGCCCGC TTTCTGGATG CGCAGAGAGT TTTGAAGGTG 
CCTGTGAGGA CCGCAGCTCT TCTGTTCGGC TGCAGTAGTG CTGCTCTCAG TGTGCTTGGT 
CAGCAGGAGG GAACGGGCAT CATCCTCAGC TACCTGACCG CTGGATGTCC ATTGGTGCTG 
GGTAACCTGT GGGATGTGAC AGATCGTGAC TTGGATCGAT TCACATCGGC TCTGCTCCAG 
TCTTGGCTCT CTGCTGGTTC TGGCTCTTCA CTTCTTCAGC ATTTGGCGAA ATCACGCAGC 
GCAACTCATT TAAAACACAT AATCGGTGCT GCGCCCATTG CGTATGGTTT ACCGGTGCAT 
ATACACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021332895.1
CDS	210..6758
Translation	MALKSLKTEDYVQQLSCVKDTIVLHNELKKHVDGGLGPYGRTMCDRIIRACNQRLGNGAVDAGLQERIVELVELAVQGFQLLEESGMQSTPFYLEKIVFHILQKVASLGAHGPACRLGQLVYRRLKCLSAEIDDFNVLVRNCFAVLWNSLLSNKPSASTLPPRDRLHCQLQALTFRLLEQGSSSSSSPSKVPLFVEEAVVEYERSCGGFTQDDVSFLTSELRELFSGAFINPQDSASTSSLLAVWCEVAFKVSKLLCKSSFSPEAVELLRGTLKEVNGHVGLRSALGLADRAVQMQCVLSSGGECSKSFTECARALRMLPCAMSSSESHALLEACQLVVWTLEASQCKGMDVTTLLACFSFLEEYQEFLLAQQKNSFSLQVQYSLCFSFYQGFICTYDSLHASQVSVGETLDRVLLFCRSTAGRMLAELRTLNNDNLLLKAVCAVNNVVYELFNRKLYEGAFGLSMIVCQELCKDCPPSLPMDRVNRCFMLTVQCSRRAGHLEQALDWIVRWIQALGSQILDHLTEPVSMWVKTKCDAARAGEDDTRLRTLRDGLGESLVDEEVMMCLLEEELRVYKEQTGDTAQERYNTLCDLLEICHEETPHTLRRATYLCEMAQVVCYQDFSQQTDCSAVDFTHEALRLLEAEPETAENTDRLKDEKAHASLWLYICTLESNLQEAVDTEKRLCAVQEENKRDVNMDPVPTNDLEYEDKQKSQESQLVYDGLRFNLLAHSKQSEPLDKCLSLWRSLLKGAVPAVRDPKLTASSMTLMAALYTLMGKHLQALEGYQLAATLFHVLGDGQSSANAFCHSTRILLYLGSPQLAQVDLEKAEKSLSSVTISEGTSIISMLAMLQKAQLHYALGQVESGVSCLMEVIKESAQHHSKSWYLLRARALQTASEYLSLDTLALNSQLRQSIMQQGLKTPDTAQYEGLKLLCSLVMMLLGNGFYGAPGPNTDTRFVDKGDSVVFKWLLLSEVLVCSERMVITRSSSGAVHEAKAQCLEALKLATKLQTLSHCAELLVLKAELELMKGATEASSLDLEQVRHLLDLCTDFGQGRQQKSEVKIKPRKGRPAAPSSSGHISEEEDDLSGILSSRALPKEPVENMARLGSQGASPPLKPKCQHGLSCLTHEETCSCLCCTELSVARVSIHWALLQADLQTDPERSLRLRQSARKRCRYVVTKLQRSLAALVHSKKSPNVCLSLLQAEQGKVHLGAVLQLLRTGDKGKAAILWEEIEAGLEAVTPKGALTPELGPIRATLLGAKAVACCLALAMKKQCIPEELFSSLWHWNPPKVKPQLKSKHRSKSPDAAPLEAGVKHNPKVKSGLEENESRKTKETTVISKKAKDSVPKITITKSSMVFKTPKATRTTRPKSVSTSTGISDLRAFDFNNEVPDISVNFTPSLHRSTNQSATAKSKASSKGSFQVYKDSSPAAEKAVVVPAAPKRTKRSRFKVEFSDESDNDAAPLAVMEKSEKKKNTFKTSRTLKPTLNTIPSDTKLVSSTVDPEPSRRTRTTKKSTALPSIGGLSSEEELGVSSQTRTRRGRPKKSTSSADTEEPERMRMIKEEEQRVLLNVSMEELRGSDTETKDTGSPDADCEVLRRDLGADFGRECFSELRRAGQNGSGSHTALPHATTSPADLSVETVQSYLRSSWLLLHHFPFPSLYPHICSLLAQSFGQTDPIITAMLHAQALGVSTRHHMTRHVVNQFRKLKKSCNDVAEGLGALSLEETSGATQSKKLSALEQIFSFTASNPMQFPQTHCQQFTQQLEDLPAGVTVCLLSLTGVHPDEIGNTILLTRLERGSTPITVRIPTADREQSVAGLLEEMDGVLKGQKEVSTVAEKSQWWDGRKALDARVEKMLEEMEEALGVWRTLFLPLTSDPKLDVQVKSLQKALKSTKITRDTLKVILSASPLLSLPDLQCLLEGMNVQDKDLLKLLQRGVAELRGREEPQGHTVLILDKYLQRLPWENIACLKSQSVTRMPSLHAVLGHSHLREVDTSCVLSCGVNPKQVYYVLNPDGNLPDTENRFKEWFTGERTWQGVCGRAPDPEKLQEAVTTKDLYIYIGHGAGARFLDAQRVLKVPVRTAALLFGCSSAALSVLGQQEGTGIILSYLTAGCPLVLGNLWDVTDRDLDRFTSALLQSWLSAGSGSSLLQHLAKSRSATHLKHIIGAAPIAYGLPVHIH

Target ORF information:

RefSeq Version	XM_021477220.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio extra spindle pole bodies like 1, separase (espl1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021477220.1

ORF Insert Sequence:

ATGGCATTAA AGAGCTTGAA AACAGAAGAT TACGTTCAGC AGTTATCCTG TGTCAAGGAC 
ACCATAGTCC TCCACAACGA ACTGAAGAAA CATGTGGATG GTGGCTTGGG TCCTTATGGT 
CGCACAATGT GTGATCGTAT CATCAGAGCC TGCAACCAGC GGCTGGGCAA CGGCGCTGTG 
GATGCTGGCC TTCAGGAGCG GATAGTGGAG CTGGTGGAAC TGGCTGTCCA AGGGTTTCAA 
CTGCTTGAAG AGTCCGGCAT GCAGAGCACC CCCTTTTATC TGGAGAAAAT AGTCTTTCAC 
ATCCTACAGA AAGTCGCAAG CCTTGGAGCT CATGGTCCTG CTTGTCGACT GGGTCAGCTC 
GTGTATAGAA GACTGAAATG TTTGTCTGCA GAGATTGATG ACTTTAATGT CCTGGTGCGG 
AATTGCTTTG CAGTCTTGTG GAACAGTCTC CTCTCTAACA AACCGAGTGC TTCCACCCTC 
CCTCCACGAG ACAGACTCCA CTGTCAGCTG CAAGCTTTAA CATTCAGACT CCTTGAACAG 
GGCTCCAGCT CGTCCTCTTC CCCATCCAAA GTTCCTCTGT TCGTGGAGGA AGCAGTAGTG 
GAGTACGAAC GATCATGTGG AGGTTTTACT CAAGACGATG TGAGTTTTCT GACCTCTGAA 
TTGCGTGAGC TGTTCTCTGG TGCTTTCATC AATCCTCAGG ACTCTGCATC AACTTCTTCA 
CTGCTGGCTG TGTGGTGTGA GGTGGCATTT AAAGTGTCTA AACTGCTCTG CAAAAGTAGC 
TTTTCACCTG AGGCTGTTGA ACTCTTGCGA GGCACTCTGA AGGAGGTTAA TGGCCACGTT 
GGCCTGCGTT CGGCTCTGGG TCTGGCAGAC CGGGCAGTGC AGATGCAGTG TGTGTTAAGT 
TCTGGAGGTG AATGCAGTAA GAGCTTCACT GAATGTGCCC GTGCTCTCCG GATGTTGCCT 
TGTGCCATGT CCAGCTCAGA GTCTCATGCC CTGTTAGAGG CTTGTCAGCT GGTGGTTTGG 
ACGTTGGAAG CCAGTCAGTG TAAAGGAATG GATGTGACCA CTTTGCTGGC TTGTTTTTCC 
TTTTTGGAAG AATATCAGGA ATTTCTGCTT GCACAGCAAA AGAACTCGTT TTCGCTGCAG 
GTCCAATACT CTCTTTGTTT CAGTTTTTAC CAGGGCTTCA TCTGCACATA TGATAGTCTT 
CATGCTTCTC AGGTGTCTGT GGGCGAGACC TTGGACAGAG TGCTATTGTT CTGCCGGTCG 
ACCGCAGGAC GTATGCTTGC TGAACTGCGG ACACTCAATA ATGACAACTT GCTGCTTAAA 
GCAGTGTGTG CAGTGAATAA TGTGGTGTAC GAGCTGTTTA ACAGGAAGCT GTATGAAGGA 
GCCTTTGGAT TGTCGATGAT TGTGTGTCAG GAGCTCTGTA AGGACTGCCC GCCCTCTCTT 
CCTATGGATC GGGTCAACCG CTGTTTCATG CTGACAGTGC AGTGTAGTCG CCGCGCAGGC 
CATCTGGAGC AGGCTCTGGA CTGGATTGTT CGCTGGATTC AGGCTTTGGG CTCACAGATT 
TTGGATCATC TCACTGAACC TGTGTCGATG TGGGTCAAAA CTAAGTGTGA TGCCGCCAGA 
GCAGGCGAGG ATGACACACG CCTGAGGACT CTGAGGGACG GGTTAGGAGA ATCCTTAGTG 
GATGAGGAGG TTATGATGTG TTTGTTAGAG GAGGAGTTGC GTGTATATAA GGAGCAGACT 
GGAGACACAG CACAGGAACG CTACAACACA CTCTGCGACC TCTTGGAAAT CTGCCATGAA 
GAAACCCCAC ACACACTAAG AAGAGCTACG TATCTGTGTG AGATGGCACA GGTTGTGTGC 
TATCAGGACT TCAGCCAGCA GACTGACTGC TCAGCAGTTG ATTTTACCCA CGAGGCTTTG 
CGGTTGCTGG AAGCAGAACC AGAGACGGCA GAGAATACTG ATAGGCTGAA GGATGAGAAA 
GCCCACGCTT CCCTGTGGCT CTATATCTGC ACTCTTGAGT CTAACCTGCA GGAGGCTGTG 
GACACAGAGA AGCGTTTGTG TGCAGTGCAG GAGGAGAATA AAAGAGATGT GAATATGGAT 
CCTGTTCCCA CAAATGATCT GGAGTACGAG GACAAACAGA AATCACAAGA GAGCCAGTTA 
GTCTACGATG GACTACGCTT CAACCTGCTG GCTCACAGCA AGCAAAGTGA ACCTCTAGAT 
AAATGTTTGT CTCTATGGCG GAGTCTCCTC AAGGGGGCTG TGCCTGCAGT TAGAGACCCT 
AAACTCACAG CTTCTTCCAT GACTTTGATG GCTGCCCTAT ACACACTTAT GGGCAAGCAC 
CTGCAAGCTC TGGAGGGATA TCAGCTTGCT GCCACCCTTT TCCATGTACT TGGTGATGGC 
CAGAGTAGTG CCAATGCCTT CTGCCACTCT ACCAGGATCT TGCTTTACCT GGGTTCACCG 
CAGCTTGCAC AGGTGGACTT AGAAAAAGCA GAAAAGAGCT TGTCATCTGT CACCATCTCT 
GAGGGCACAT CTATTATCTC CATGCTGGCC ATGTTGCAAA AAGCACAGCT ACACTATGCT 
CTAGGACAGG TGGAGAGTGG CGTGTCCTGC CTGATGGAGG TGATAAAGGA ATCTGCTCAG 
CATCATTCTA AGAGCTGGTA CCTGCTCAGG GCTCGAGCAC TGCAGACAGC CAGTGAATAC 
TTGAGTCTGG ACACACTGGC TTTGAACTCA CAGCTACGCC AGAGCATCAT GCAGCAGGGT 
CTAAAGACTC CAGACACTGC TCAATATGAA GGGTTGAAGC TGTTGTGTAG CCTGGTGATG 
ATGTTATTGG GCAACGGGTT TTACGGAGCG CCTGGACCAA ACACTGACAC TCGCTTTGTG 
GATAAAGGAG ACAGTGTTGT GTTTAAGTGG CTGCTGCTGA GTGAAGTGCT GGTGTGCTCG 
GAGAGAATGG TGATCACCAG GAGCAGCAGC GGAGCCGTTC ATGAAGCTAA AGCTCAGTGT 
CTAGAGGCTC TCAAACTGGC CACCAAACTA CAGACACTCA GCCACTGTGC TGAGCTGTTG 
GTGCTGAAGG CAGAGCTGGA GCTGATGAAG GGTGCAACTG AAGCTAGTAG TTTAGACCTA 
GAGCAAGTTA GACATTTACT TGATCTCTGC ACAGATTTTG GCCAGGGACG ACAACAGAAA 
TCTGAAGTGA AAATCAAACC ACGCAAAGGT CGTCCCGCTG CCCCTTCTTC CTCTGGTCAC 
ATCTCTGAGG AAGAGGATGA TCTGAGTGGG ATCCTCAGCT CTCGTGCCCT TCCGAAGGAG 
CCTGTGGAGA ACATGGCTAG ATTAGGAAGC CAAGGAGCTT CTCCCCCTCT AAAGCCCAAA 
TGCCAGCATG GCCTCTCCTG TCTGACCCAT GAAGAGACCT GCTCTTGTCT CTGCTGCACT 
GAGCTCAGCG TGGCTCGAGT CAGCATCCAC TGGGCACTAT TACAGGCAGA TCTCCAGACA 
GATCCCGAAC GCTCTCTCAG ACTACGCCAG TCTGCCAGGA AACGCTGCCG CTATGTTGTA 
ACTAAACTTC AGAGAAGTTT GGCAGCACTT GTACACTCCA AAAAATCACC TAATGTCTGC 
TTGTCACTGC TTCAGGCTGA ACAGGGTAAA GTGCATCTGG GTGCTGTGCT TCAACTGCTC 
AGAACTGGAG ACAAAGGGAA AGCTGCCATC TTGTGGGAGG AGATAGAAGC AGGATTAGAG 
GCAGTGACAC CTAAAGGAGC ACTGACACCA GAACTTGGGC CTATCAGAGC TACTCTACTG 
GGGGCTAAAG CCGTAGCCTG CTGCTTGGCG TTGGCAATGA AGAAACAGTG TATACCCGAA 
GAGCTGTTTT CTTCATTGTG GCACTGGAAT CCACCAAAGG TTAAACCGCA ATTAAAATCT 
AAGCATCGAT CCAAGAGTCC GGACGCAGCT CCTCTAGAGG CAGGTGTTAA ACATAACCCT 
AAAGTTAAGA GTGGACTTGA GGAGAATGAA AGCAGAAAAA CCAAAGAGAC CACTGTCATT 
TCGAAGAAAG CTAAAGACTC TGTGCCCAAA ATTACCATCA CAAAATCCTC CATGGTTTTT 
AAAACGCCCA AAGCAACGAG AACAACCCGG CCCAAATCTG TCTCCACTAG CACTGGCATC 
AGTGACCTGA GAGCATTTGA CTTCAATAAT GAGGTGCCTG ACATTTCAGT CAACTTCACA 
CCATCATTAC ACCGATCTAC AAACCAAAGT GCGACTGCAA AGTCAAAAGC GTCCTCAAAG 
GGTTCGTTCC AGGTTTACAA AGATTCTTCT CCTGCTGCAG AGAAAGCTGT AGTTGTCCCG 
GCTGCTCCTA AACGAACAAA GCGTTCTCGC TTCAAGGTTG AGTTCAGCGA TGAAAGCGAC 
AACGATGCTG CTCCTCTTGC TGTAATGGAA AAGTCAGAGA AAAAGAAAAA CACCTTTAAA 
ACATCACGCA CCTTGAAACC AACCCTTAAC ACCATCCCTA GTGACACCAA ACTTGTGAGC 
TCCACAGTAG ATCCAGAACC CTCCAGACGA ACACGGACCA CTAAGAAAAG CACAGCTCTT 
CCCTCCATTG GTGGCCTCTC TTCTGAGGAA GAACTTGGAG TATCTTCACA GACACGTACG 
CGTAGAGGAC GACCAAAGAA GAGCACCAGC TCAGCAGACA CAGAGGAGCC AGAGAGGATG 
AGGATGATTA AGGAGGAAGA ACAGAGGGTT CTTTTGAATG TCAGCATGGA GGAGCTCAGA 
GGCTCTGACA CTGAGACAAA AGACACTGGA AGTCCAGATG CTGACTGTGA GGTCTTGAGG 
AGAGATCTAG GTGCAGATTT TGGGCGAGAG TGTTTCAGTG AGCTCAGGAG AGCTGGACAG 
AATGGATCAG GATCTCACAC AGCTCTGCCA CATGCAACTA CATCTCCTGC AGACTTGTCA 
GTAGAGACGG TCCAGTCATA CCTGCGCTCC TCATGGCTCC TCCTGCACCA CTTCCCGTTT 
CCTTCTCTTT ATCCTCACAT CTGCTCATTA CTCGCTCAGT CATTCGGTCA AACGGACCCC 
ATCATCACAG CAATGCTGCA TGCACAGGCA CTGGGAGTGT CCACTCGCCA CCATATGACC 
CGTCACGTGG TTAACCAGTT CAGAAAGTTG AAGAAATCCT GTAACGATGT AGCAGAAGGT 
CTTGGAGCTC TGAGTCTGGA GGAAACATCT GGAGCAACGC AGTCAAAGAA ACTCTCTGCG 
TTGGAGCAGA TCTTTTCATT CACAGCATCA AACCCAATGC AGTTTCCACA GACACACTGT 
CAGCAGTTCA CACAGCAGCT GGAAGATCTA CCTGCAGGAG TGACCGTATG CTTGCTGTCA 
CTGACGGGGG TGCATCCTGA TGAAATTGGC AACACTATTC TTCTGACCCG ACTGGAGCGC 
GGCTCTACCC CAATCACAGT TCGAATCCCC ACTGCAGACA GAGAGCAATC TGTTGCTGGC 
CTGTTGGAAG AAATGGATGG AGTCTTGAAA GGACAAAAGG AAGTGAGCAC AGTGGCTGAG 
AAATCACAGT GGTGGGACGG AAGGAAGGCT TTAGATGCCC GTGTGGAGAA AATGCTGGAG 
GAGATGGAAG AGGCTTTAGG AGTGTGGCGA ACTCTTTTTC TGCCTTTGAC CTCTGATCCT 
AAGCTTGATG TTCAGGTAAA GAGCCTCCAA AAGGCTCTTA AATCAACAAA GATCACACGG 
GACACTCTCA AGGTGATTCT ATCAGCGTCT CCACTGCTGT CCCTGCCAGA CCTGCAGTGT 
CTTCTGGAAG GAATGAATGT GCAGGACAAG GACCTGCTGA AGCTCCTGCA GAGAGGCGTG 
GCTGAACTGA GAGGGAGGGA GGAGCCACAG GGACACACTG TTCTGATTTT AGACAAGTAC 
TTGCAGAGGT TGCCGTGGGA GAACATTGCT TGTCTGAAGT CTCAGTCGGT CACTCGAATG 
CCCTCATTAC ACGCGGTGCT TGGACACAGT CATCTGAGAG AGGTGGATAC AAGTTGTGTT 
CTATCTTGTG GTGTGAACCC TAAGCAGGTT TACTATGTGC TAAACCCAGA TGGAAACCTG 
CCCGACACTG AGAATCGCTT CAAAGAGTGG TTTACAGGTG AACGAACATG GCAGGGGGTT 
TGTGGAAGGG CTCCTGATCC GGAGAAACTA CAAGAGGCTG TGACCACTAA AGATCTCTAC 
ATATACATCG GACATGGTGC AGGAGCCCGC TTTCTGGATG CGCAGAGAGT TTTGAAGGTG 
CCTGTGAGGA CCGCAGCTCT TCTGTTCGGC TGCAGTAGTG CTGCTCTCAG TGTGCTTGGT 
CAGCAGGAGG GAACGGGCAT CATCCTCAGC TACCTGACCG CTGGATGTCC ATTGGTGCTG 
GGTAACCTGT GGGATGTGAC AGATCGTGAC TTGGATCGAT TCACATCGGC TCTGCTCCAG 
TCTTGGCTCT CTGCTGGTTC TGGCTCTTCA CTTCTTCAGC ATTTGGCGAA ATCACGCAGC 
GCAACTCATT TAAAACACAT AATCGGTGCT GCGCCCATTG CGTATGGTTT ACCGGTGCAT 
ATACACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

espl1 ( XM_001337833.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_001337869.1 Danio rerio extra spindle pole bodies like 1, separase (espl1), transcript variant X1, mRNA.

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CloneID	ODa12112
Clone ID Related Accession (Same CDS sequence)	XM_021477220.1 , XM_001337833.4 , XM_001337833.5 , XM_017356662.1
Accession Version	XM_001337833.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6549bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	separin isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_001337833.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCATTAA AGAGCTTGAA AACAGAAGAT TACGTTCAGC AGTTATCCTG TGTCAAGGAC 
ACCATAGTCC TCCACAACGA ACTGAAGAAA CATGTGGATG GTGGCTTGGG TCCTTATGGT 
CGCACAATGT GTGATCGTAT CATCAGAGCC TGCAACCAGC GGCTGGGCAA CGGCGCTGTG 
GATGCTGGCC TTCAGGAGCG GATAGTGGAG CTGGTGGAAC TGGCTGTCCA AGGGTTTCAA 
CTGCTTGAAG AGTCCGGCAT GCAGAGCACC CCCTTTTATC TGGAGAAAAT AGTCTTTCAC 
ATCCTACAGA AAGTCGCAAG CCTTGGAGCT CATGGTCCTG CTTGTCGACT GGGTCAGCTC 
GTGTATAGAA GACTGAAATG TTTGTCTGCA GAGATTGATG ACTTTAATGT CCTGGTGCGG 
AATTGCTTTG CAGTCTTGTG GAACAGTCTC CTCTCTAACA AACCGAGTGC TTCCACCCTC 
CCTCCACGAG ACAGACTCCA CTGTCAGCTG CAAGCTTTAA CATTCAGACT CCTTGAACAG 
GGCTCCAGCT CGTCCTCTTC CCCATCCAAA GTTCCTCTGT TCGTGGAGGA AGCAGTAGTG 
GAGTACGAAC GATCATGTGG AGGTTTTACT CAAGACGATG TGAGTTTTCT GACCTCTGAA 
TTGCGTGAGC TGTTCTCTGG TGCTTTCATC AATCCTCAGG ACTCTGCATC AACTTCTTCA 
CTGCTGGCTG TGTGGTGTGA GGTGGCATTT AAAGTGTCTA AACTGCTCTG CAAAAGTAGC 
TTTTCACCTG AGGCTGTTGA ACTCTTGCGA GGCACTCTGA AGGAGGTTAA TGGCCACGTT 
GGCCTGCGTT CGGCTCTGGG TCTGGCAGAC CGGGCAGTGC AGATGCAGTG TGTGTTAAGT 
TCTGGAGGTG AATGCAGTAA GAGCTTCACT GAATGTGCCC GTGCTCTCCG GATGTTGCCT 
TGTGCCATGT CCAGCTCAGA GTCTCATGCC CTGTTAGAGG CTTGTCAGCT GGTGGTTTGG 
ACGTTGGAAG CCAGTCAGTG TAAAGGAATG GATGTGACCA CTTTGCTGGC TTGTTTTTCC 
TTTTTGGAAG AATATCAGGA ATTTCTGCTT GCACAGCAAA AGAACTCGTT TTCGCTGCAG 
GTCCAATACT CTCTTTGTTT CAGTTTTTAC CAGGGCTTCA TCTGCACATA TGATAGTCTT 
CATGCTTCTC AGGTGTCTGT GGGCGAGACC TTGGACAGAG TGCTATTGTT CTGCCGGTCG 
ACCGCAGGAC GTATGCTTGC TGAACTGCGG ACACTCAATA ATGACAACTT GCTGCTTAAA 
GCAGTGTGTG CAGTGAATAA TGTGGTGTAC GAGCTGTTTA ACAGGAAGCT GTATGAAGGA 
GCCTTTGGAT TGTCGATGAT TGTGTGTCAG GAGCTCTGTA AGGACTGCCC GCCCTCTCTT 
CCTATGGATC GGGTCAACCG CTGTTTCATG CTGACAGTGC AGTGTAGTCG CCGCGCAGGC 
CATCTGGAGC AGGCTCTGGA CTGGATTGTT CGCTGGATTC AGGCTTTGGG CTCACAGATT 
TTGGATCATC TCACTGAACC TGTGTCGATG TGGGTCAAAA CTAAGTGTGA TGCCGCCAGA 
GCAGGCGAGG ATGACACACG CCTGAGGACT CTGAGGGACG GGTTAGGAGA ATCCTTAGTG 
GATGAGGAGG TTATGATGTG TTTGTTAGAG GAGGAGTTGC GTGTATATAA GGAGCAGACT 
GGAGACACAG CACAGGAACG CTACAACACA CTCTGCGACC TCTTGGAAAT CTGCCATGAA 
GAAACCCCAC ACACACTAAG AAGAGCTACG TATCTGTGTG AGATGGCACA GGTTGTGTGC 
TATCAGGACT TCAGCCAGCA GACTGACTGC TCAGCAGTTG ATTTTACCCA CGAGGCTTTG 
CGGTTGCTGG AAGCAGAACC AGAGACGGCA GAGAATACTG ATAGGCTGAA GGATGAGAAA 
GCCCACGCTT CCCTGTGGCT CTATATCTGC ACTCTTGAGT CTAACCTGCA GGAGGCTGTG 
GACACAGAGA AGCGTTTGTG TGCAGTGCAG GAGGAGAATA AAAGAGATGT GAATATGGAT 
CCTGTTCCCA CAAATGATCT GGAGTACGAG GACAAACAGA AATCACAAGA GAGCCAGTTA 
GTCTACGATG GACTACGCTT CAACCTGCTG GCTCACAGCA AGCAAAGTGA ACCTCTAGAT 
AAATGTTTGT CTCTATGGCG GAGTCTCCTC AAGGGGGCTG TGCCTGCAGT TAGAGACCCT 
AAACTCACAG CTTCTTCCAT GACTTTGATG GCTGCCCTAT ACACACTTAT GGGCAAGCAC 
CTGCAAGCTC TGGAGGGATA TCAGCTTGCT GCCACCCTTT TCCATGTACT TGGTGATGGC 
CAGAGTAGTG CCAATGCCTT CTGCCACTCT ACCAGGATCT TGCTTTACCT GGGTTCACCG 
CAGCTTGCAC AGGTGGACTT AGAAAAAGCA GAAAAGAGCT TGTCATCTGT CACCATCTCT 
GAGGGCACAT CTATTATCTC CATGCTGGCC ATGTTGCAAA AAGCACAGCT ACACTATGCT 
CTAGGACAGG TGGAGAGTGG CGTGTCCTGC CTGATGGAGG TGATAAAGGA ATCTGCTCAG 
CATCATTCTA AGAGCTGGTA CCTGCTCAGG GCTCGAGCAC TGCAGACAGC CAGTGAATAC 
TTGAGTCTGG ACACACTGGC TTTGAACTCA CAGCTACGCC AGAGCATCAT GCAGCAGGGT 
CTAAAGACTC CAGACACTGC TCAATATGAA GGGTTGAAGC TGTTGTGTAG CCTGGTGATG 
ATGTTATTGG GCAACGGGTT TTACGGAGCG CCTGGACCAA ACACTGACAC TCGCTTTGTG 
GATAAAGGAG ACAGTGTTGT GTTTAAGTGG CTGCTGCTGA GTGAAGTGCT GGTGTGCTCG 
GAGAGAATGG TGATCACCAG GAGCAGCAGC GGAGCCGTTC ATGAAGCTAA AGCTCAGTGT 
CTAGAGGCTC TCAAACTGGC CACCAAACTA CAGACACTCA GCCACTGTGC TGAGCTGTTG 
GTGCTGAAGG CAGAGCTGGA GCTGATGAAG GGTGCAACTG AAGCTAGTAG TTTAGACCTA 
GAGCAAGTTA GACATTTACT TGATCTCTGC ACAGATTTTG GCCAGGGACG ACAACAGAAA 
TCTGAAGTGA AAATCAAACC ACGCAAAGGT CGTCCCGCTG CCCCTTCTTC CTCTGGTCAC 
ATCTCTGAGG AAGAGGATGA TCTGAGTGGG ATCCTCAGCT CTCGTGCCCT TCCGAAGGAG 
CCTGTGGAGA ACATGGCTAG ATTAGGAAGC CAAGGAGCTT CTCCCCCTCT AAAGCCCAAA 
TGCCAGCATG GCCTCTCCTG TCTGACCCAT GAAGAGACCT GCTCTTGTCT CTGCTGCACT 
GAGCTCAGCG TGGCTCGAGT CAGCATCCAC TGGGCACTAT TACAGGCAGA TCTCCAGACA 
GATCCCGAAC GCTCTCTCAG ACTACGCCAG TCTGCCAGGA AACGCTGCCG CTATGTTGTA 
ACTAAACTTC AGAGAAGTTT GGCAGCACTT GTACACTCCA AAAAATCACC TAATGTCTGC 
TTGTCACTGC TTCAGGCTGA ACAGGGTAAA GTGCATCTGG GTGCTGTGCT TCAACTGCTC 
AGAACTGGAG ACAAAGGGAA AGCTGCCATC TTGTGGGAGG AGATAGAAGC AGGATTAGAG 
GCAGTGACAC CTAAAGGAGC ACTGACACCA GAACTTGGGC CTATCAGAGC TACTCTACTG 
GGGGCTAAAG CCGTAGCCTG CTGCTTGGCG TTGGCAATGA AGAAACAGTG TATACCCGAA 
GAGCTGTTTT CTTCATTGTG GCACTGGAAT CCACCAAAGG TTAAACCGCA ATTAAAATCT 
AAGCATCGAT CCAAGAGTCC GGACGCAGCT CCTCTAGAGG CAGGTGTTAA ACATAACCCT 
AAAGTTAAGA GTGGACTTGA GGAGAATGAA AGCAGAAAAA CCAAAGAGAC CACTGTCATT 
TCGAAGAAAG CTAAAGACTC TGTGCCCAAA ATTACCATCA CAAAATCCTC CATGGTTTTT 
AAAACGCCCA AAGCAACGAG AACAACCCGG CCCAAATCTG TCTCCACTAG CACTGGCATC 
AGTGACCTGA GAGCATTTGA CTTCAATAAT GAGGTGCCTG ACATTTCAGT CAACTTCACA 
CCATCATTAC ACCGATCTAC AAACCAAAGT GCGACTGCAA AGTCAAAAGC GTCCTCAAAG 
GGTTCGTTCC AGGTTTACAA AGATTCTTCT CCTGCTGCAG AGAAAGCTGT AGTTGTCCCG 
GCTGCTCCTA AACGAACAAA GCGTTCTCGC TTCAAGGTTG AGTTCAGCGA TGAAAGCGAC 
AACGATGCTG CTCCTCTTGC TGTAATGGAA AAGTCAGAGA AAAAGAAAAA CACCTTTAAA 
ACATCACGCA CCTTGAAACC AACCCTTAAC ACCATCCCTA GTGACACCAA ACTTGTGAGC 
TCCACAGTAG ATCCAGAACC CTCCAGACGA ACACGGACCA CTAAGAAAAG CACAGCTCTT 
CCCTCCATTG GTGGCCTCTC TTCTGAGGAA GAACTTGGAG TATCTTCACA GACACGTACG 
CGTAGAGGAC GACCAAAGAA GAGCACCAGC TCAGCAGACA CAGAGGAGCC AGAGAGGATG 
AGGATGATTA AGGAGGAAGA ACAGAGGGTT CTTTTGAATG TCAGCATGGA GGAGCTCAGA 
GGCTCTGACA CTGAGACAAA AGACACTGGA AGTCCAGATG CTGACTGTGA GGTCTTGAGG 
AGAGATCTAG GTGCAGATTT TGGGCGAGAG TGTTTCAGTG AGCTCAGGAG AGCTGGACAG 
AATGGATCAG GATCTCACAC AGCTCTGCCA CATGCAACTA CATCTCCTGC AGACTTGTCA 
GTAGAGACGG TCCAGTCATA CCTGCGCTCC TCATGGCTCC TCCTGCACCA CTTCCCGTTT 
CCTTCTCTTT ATCCTCACAT CTGCTCATTA CTCGCTCAGT CATTCGGTCA AACGGACCCC 
ATCATCACAG CAATGCTGCA TGCACAGGCA CTGGGAGTGT CCACTCGCCA CCATATGACC 
CGTCACGTGG TTAACCAGTT CAGAAAGTTG AAGAAATCCT GTAACGATGT AGCAGAAGGT 
CTTGGAGCTC TGAGTCTGGA GGAAACATCT GGAGCAACGC AGTCAAAGAA ACTCTCTGCG 
TTGGAGCAGA TCTTTTCATT CACAGCATCA AACCCAATGC AGTTTCCACA GACACACTGT 
CAGCAGTTCA CACAGCAGCT GGAAGATCTA CCTGCAGGAG TGACCGTATG CTTGCTGTCA 
CTGACGGGGG TGCATCCTGA TGAAATTGGC AACACTATTC TTCTGACCCG ACTGGAGCGC 
GGCTCTACCC CAATCACAGT TCGAATCCCC ACTGCAGACA GAGAGCAATC TGTTGCTGGC 
CTGTTGGAAG AAATGGATGG AGTCTTGAAA GGACAAAAGG AAGTGAGCAC AGTGGCTGAG 
AAATCACAGT GGTGGGACGG AAGGAAGGCT TTAGATGCCC GTGTGGAGAA AATGCTGGAG 
GAGATGGAAG AGGCTTTAGG AGTGTGGCGA ACTCTTTTTC TGCCTTTGAC CTCTGATCCT 
AAGCTTGATG TTCAGGTAAA GAGCCTCCAA AAGGCTCTTA AATCAACAAA GATCACACGG 
GACACTCTCA AGGTGATTCT ATCAGCGTCT CCACTGCTGT CCCTGCCAGA CCTGCAGTGT 
CTTCTGGAAG GAATGAATGT GCAGGACAAG GACCTGCTGA AGCTCCTGCA GAGAGGCGTG 
GCTGAACTGA GAGGGAGGGA GGAGCCACAG GGACACACTG TTCTGATTTT AGACAAGTAC 
TTGCAGAGGT TGCCGTGGGA GAACATTGCT TGTCTGAAGT CTCAGTCGGT CACTCGAATG 
CCCTCATTAC ACGCGGTGCT TGGACACAGT CATCTGAGAG AGGTGGATAC AAGTTGTGTT 
CTATCTTGTG GTGTGAACCC TAAGCAGGTT TACTATGTGC TAAACCCAGA TGGAAACCTG 
CCCGACACTG AGAATCGCTT CAAAGAGTGG TTTACAGGTG AACGAACATG GCAGGGGGTT 
TGTGGAAGGG CTCCTGATCC GGAGAAACTA CAAGAGGCTG TGACCACTAA AGATCTCTAC 
ATATACATCG GACATGGTGC AGGAGCCCGC TTTCTGGATG CGCAGAGAGT TTTGAAGGTG 
CCTGTGAGGA CCGCAGCTCT TCTGTTCGGC TGCAGTAGTG CTGCTCTCAG TGTGCTTGGT 
CAGCAGGAGG GAACGGGCAT CATCCTCAGC TACCTGACCG CTGGATGTCC ATTGGTGCTG 
GGTAACCTGT GGGATGTGAC AGATCGTGAC TTGGATCGAT TCACATCGGC TCTGCTCCAG 
TCTTGGCTCT CTGCTGGTTC TGGCTCTTCA CTTCTTCAGC ATTTGGCGAA ATCACGCAGC 
GCAACTCATT TAAAACACAT AATCGGTGCT GCGCCCATTG CGTATGGTTT ACCGGTGCAT 
ATACACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001337869.1
CDS	187..6735
Translation	MALKSLKTEDYVQQLSCVKDTIVLHNELKKHVDGGLGPYGRTMCDRIIRACNQRLGNGAVDAGLQERIVELVELAVQGFQLLEESGMQSTPFYLEKIVFHILQKVASLGAHGPACRLGQLVYRRLKCLSAEIDDFNVLVRNCFAVLWNSLLSNKPSASTLPPRDRLHCQLQALTFRLLEQGSSSSSSPSKVPLFVEEAVVEYERSCGGFTQDDVSFLTSELRELFSGAFINPQDSASTSSLLAVWCEVAFKVSKLLCKSSFSPEAVELLRGTLKEVNGHVGLRSALGLADRAVQMQCVLSSGGECSKSFTECARALRMLPCAMSSSESHALLEACQLVVWTLEASQCKGMDVTTLLACFSFLEEYQEFLLAQQKNSFSLQVQYSLCFSFYQGFICTYDSLHASQVSVGETLDRVLLFCRSTAGRMLAELRTLNNDNLLLKAVCAVNNVVYELFNRKLYEGAFGLSMIVCQELCKDCPPSLPMDRVNRCFMLTVQCSRRAGHLEQALDWIVRWIQALGSQILDHLTEPVSMWVKTKCDAARAGEDDTRLRTLRDGLGESLVDEEVMMCLLEEELRVYKEQTGDTAQERYNTLCDLLEICHEETPHTLRRATYLCEMAQVVCYQDFSQQTDCSAVDFTHEALRLLEAEPETAENTDRLKDEKAHASLWLYICTLESNLQEAVDTEKRLCAVQEENKRDVNMDPVPTNDLEYEDKQKSQESQLVYDGLRFNLLAHSKQSEPLDKCLSLWRSLLKGAVPAVRDPKLTASSMTLMAALYTLMGKHLQALEGYQLAATLFHVLGDGQSSANAFCHSTRILLYLGSPQLAQVDLEKAEKSLSSVTISEGTSIISMLAMLQKAQLHYALGQVESGVSCLMEVIKESAQHHSKSWYLLRARALQTASEYLSLDTLALNSQLRQSIMQQGLKTPDTAQYEGLKLLCSLVMMLLGNGFYGAPGPNTDTRFVDKGDSVVFKWLLLSEVLVCSERMVITRSSSGAVHEAKAQCLEALKLATKLQTLSHCAELLVLKAELELMKGATEASSLDLEQVRHLLDLCTDFGQGRQQKSEVKIKPRKGRPAAPSSSGHISEEEDDLSGILSSRALPKEPVENMARLGSQGASPPLKPKCQHGLSCLTHEETCSCLCCTELSVARVSIHWALLQADLQTDPERSLRLRQSARKRCRYVVTKLQRSLAALVHSKKSPNVCLSLLQAEQGKVHLGAVLQLLRTGDKGKAAILWEEIEAGLEAVTPKGALTPELGPIRATLLGAKAVACCLALAMKKQCIPEELFSSLWHWNPPKVKPQLKSKHRSKSPDAAPLEAGVKHNPKVKSGLEENESRKTKETTVISKKAKDSVPKITITKSSMVFKTPKATRTTRPKSVSTSTGISDLRAFDFNNEVPDISVNFTPSLHRSTNQSATAKSKASSKGSFQVYKDSSPAAEKAVVVPAAPKRTKRSRFKVEFSDESDNDAAPLAVMEKSEKKKNTFKTSRTLKPTLNTIPSDTKLVSSTVDPEPSRRTRTTKKSTALPSIGGLSSEEELGVSSQTRTRRGRPKKSTSSADTEEPERMRMIKEEEQRVLLNVSMEELRGSDTETKDTGSPDADCEVLRRDLGADFGRECFSELRRAGQNGSGSHTALPHATTSPADLSVETVQSYLRSSWLLLHHFPFPSLYPHICSLLAQSFGQTDPIITAMLHAQALGVSTRHHMTRHVVNQFRKLKKSCNDVAEGLGALSLEETSGATQSKKLSALEQIFSFTASNPMQFPQTHCQQFTQQLEDLPAGVTVCLLSLTGVHPDEIGNTILLTRLERGSTPITVRIPTADREQSVAGLLEEMDGVLKGQKEVSTVAEKSQWWDGRKALDARVEKMLEEMEEALGVWRTLFLPLTSDPKLDVQVKSLQKALKSTKITRDTLKVILSASPLLSLPDLQCLLEGMNVQDKDLLKLLQRGVAELRGREEPQGHTVLILDKYLQRLPWENIACLKSQSVTRMPSLHAVLGHSHLREVDTSCVLSCGVNPKQVYYVLNPDGNLPDTENRFKEWFTGERTWQGVCGRAPDPEKLQEAVTTKDLYIYIGHGAGARFLDAQRVLKVPVRTAALLFGCSSAALSVLGQQEGTGIILSYLTAGCPLVLGNLWDVTDRDLDRFTSALLQSWLSAGSGSSLLQHLAKSRSATHLKHIIGAAPIAYGLPVHIH

Target ORF information:

RefSeq Version	XM_001337833.4
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio extra spindle pole bodies like 1, separase (espl1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001337833.4

ORF Insert Sequence:

ATGGCATTAA AGAGCTTGAA AACAGAAGAT TACGTTCAGC AGTTATCCTG TGTCAAGGAC 
ACCATAGTCC TCCACAACGA ACTGAAGAAA CATGTGGATG GTGGCTTGGG TCCTTATGGT 
CGCACAATGT GTGATCGTAT CATCAGAGCC TGCAACCAGC GGCTGGGCAA CGGCGCTGTG 
GATGCTGGCC TTCAGGAGCG GATAGTGGAG CTGGTGGAAC TGGCTGTCCA AGGGTTTCAA 
CTGCTTGAAG AGTCCGGCAT GCAGAGCACC CCCTTTTATC TGGAGAAAAT AGTCTTTCAC 
ATCCTACAGA AAGTCGCAAG CCTTGGAGCT CATGGTCCTG CTTGTCGACT GGGTCAGCTC 
GTGTATAGAA GACTGAAATG TTTGTCTGCA GAGATTGATG ACTTTAATGT CCTGGTGCGG 
AATTGCTTTG CAGTCTTGTG GAACAGTCTC CTCTCTAACA AACCGAGTGC TTCCACCCTC 
CCTCCACGAG ACAGACTCCA CTGTCAGCTG CAAGCTTTAA CATTCAGACT CCTTGAACAG 
GGCTCCAGCT CGTCCTCTTC CCCATCCAAA GTTCCTCTGT TCGTGGAGGA AGCAGTAGTG 
GAGTACGAAC GATCATGTGG AGGTTTTACT CAAGACGATG TGAGTTTTCT GACCTCTGAA 
TTGCGTGAGC TGTTCTCTGG TGCTTTCATC AATCCTCAGG ACTCTGCATC AACTTCTTCA 
CTGCTGGCTG TGTGGTGTGA GGTGGCATTT AAAGTGTCTA AACTGCTCTG CAAAAGTAGC 
TTTTCACCTG AGGCTGTTGA ACTCTTGCGA GGCACTCTGA AGGAGGTTAA TGGCCACGTT 
GGCCTGCGTT CGGCTCTGGG TCTGGCAGAC CGGGCAGTGC AGATGCAGTG TGTGTTAAGT 
TCTGGAGGTG AATGCAGTAA GAGCTTCACT GAATGTGCCC GTGCTCTCCG GATGTTGCCT 
TGTGCCATGT CCAGCTCAGA GTCTCATGCC CTGTTAGAGG CTTGTCAGCT GGTGGTTTGG 
ACGTTGGAAG CCAGTCAGTG TAAAGGAATG GATGTGACCA CTTTGCTGGC TTGTTTTTCC 
TTTTTGGAAG AATATCAGGA ATTTCTGCTT GCACAGCAAA AGAACTCGTT TTCGCTGCAG 
GTCCAATACT CTCTTTGTTT CAGTTTTTAC CAGGGCTTCA TCTGCACATA TGATAGTCTT 
CATGCTTCTC AGGTGTCTGT GGGCGAGACC TTGGACAGAG TGCTATTGTT CTGCCGGTCG 
ACCGCAGGAC GTATGCTTGC TGAACTGCGG ACACTCAATA ATGACAACTT GCTGCTTAAA 
GCAGTGTGTG CAGTGAATAA TGTGGTGTAC GAGCTGTTTA ACAGGAAGCT GTATGAAGGA 
GCCTTTGGAT TGTCGATGAT TGTGTGTCAG GAGCTCTGTA AGGACTGCCC GCCCTCTCTT 
CCTATGGATC GGGTCAACCG CTGTTTCATG CTGACAGTGC AGTGTAGTCG CCGCGCAGGC 
CATCTGGAGC AGGCTCTGGA CTGGATTGTT CGCTGGATTC AGGCTTTGGG CTCACAGATT 
TTGGATCATC TCACTGAACC TGTGTCGATG TGGGTCAAAA CTAAGTGTGA TGCCGCCAGA 
GCAGGCGAGG ATGACACACG CCTGAGGACT CTGAGGGACG GGTTAGGAGA ATCCTTAGTG 
GATGAGGAGG TTATGATGTG TTTGTTAGAG GAGGAGTTGC GTGTATATAA GGAGCAGACT 
GGAGACACAG CACAGGAACG CTACAACACA CTCTGCGACC TCTTGGAAAT CTGCCATGAA 
GAAACCCCAC ACACACTAAG AAGAGCTACG TATCTGTGTG AGATGGCACA GGTTGTGTGC 
TATCAGGACT TCAGCCAGCA GACTGACTGC TCAGCAGTTG ATTTTACCCA CGAGGCTTTG 
CGGTTGCTGG AAGCAGAACC AGAGACGGCA GAGAATACTG ATAGGCTGAA GGATGAGAAA 
GCCCACGCTT CCCTGTGGCT CTATATCTGC ACTCTTGAGT CTAACCTGCA GGAGGCTGTG 
GACACAGAGA AGCGTTTGTG TGCAGTGCAG GAGGAGAATA AAAGAGATGT GAATATGGAT 
CCTGTTCCCA CAAATGATCT GGAGTACGAG GACAAACAGA AATCACAAGA GAGCCAGTTA 
GTCTACGATG GACTACGCTT CAACCTGCTG GCTCACAGCA AGCAAAGTGA ACCTCTAGAT 
AAATGTTTGT CTCTATGGCG GAGTCTCCTC AAGGGGGCTG TGCCTGCAGT TAGAGACCCT 
AAACTCACAG CTTCTTCCAT GACTTTGATG GCTGCCCTAT ACACACTTAT GGGCAAGCAC 
CTGCAAGCTC TGGAGGGATA TCAGCTTGCT GCCACCCTTT TCCATGTACT TGGTGATGGC 
CAGAGTAGTG CCAATGCCTT CTGCCACTCT ACCAGGATCT TGCTTTACCT GGGTTCACCG 
CAGCTTGCAC AGGTGGACTT AGAAAAAGCA GAAAAGAGCT TGTCATCTGT CACCATCTCT 
GAGGGCACAT CTATTATCTC CATGCTGGCC ATGTTGCAAA AAGCACAGCT ACACTATGCT 
CTAGGACAGG TGGAGAGTGG CGTGTCCTGC CTGATGGAGG TGATAAAGGA ATCTGCTCAG 
CATCATTCTA AGAGCTGGTA CCTGCTCAGG GCTCGAGCAC TGCAGACAGC CAGTGAATAC 
TTGAGTCTGG ACACACTGGC TTTGAACTCA CAGCTACGCC AGAGCATCAT GCAGCAGGGT 
CTAAAGACTC CAGACACTGC TCAATATGAA GGGTTGAAGC TGTTGTGTAG CCTGGTGATG 
ATGTTATTGG GCAACGGGTT TTACGGAGCG CCTGGACCAA ACACTGACAC TCGCTTTGTG 
GATAAAGGAG ACAGTGTTGT GTTTAAGTGG CTGCTGCTGA GTGAAGTGCT GGTGTGCTCG 
GAGAGAATGG TGATCACCAG GAGCAGCAGC GGAGCCGTTC ATGAAGCTAA AGCTCAGTGT 
CTAGAGGCTC TCAAACTGGC CACCAAACTA CAGACACTCA GCCACTGTGC TGAGCTGTTG 
GTGCTGAAGG CAGAGCTGGA GCTGATGAAG GGTGCAACTG AAGCTAGTAG TTTAGACCTA 
GAGCAAGTTA GACATTTACT TGATCTCTGC ACAGATTTTG GCCAGGGACG ACAACAGAAA 
TCTGAAGTGA AAATCAAACC ACGCAAAGGT CGTCCCGCTG CCCCTTCTTC CTCTGGTCAC 
ATCTCTGAGG AAGAGGATGA TCTGAGTGGG ATCCTCAGCT CTCGTGCCCT TCCGAAGGAG 
CCTGTGGAGA ACATGGCTAG ATTAGGAAGC CAAGGAGCTT CTCCCCCTCT AAAGCCCAAA 
TGCCAGCATG GCCTCTCCTG TCTGACCCAT GAAGAGACCT GCTCTTGTCT CTGCTGCACT 
GAGCTCAGCG TGGCTCGAGT CAGCATCCAC TGGGCACTAT TACAGGCAGA TCTCCAGACA 
GATCCCGAAC GCTCTCTCAG ACTACGCCAG TCTGCCAGGA AACGCTGCCG CTATGTTGTA 
ACTAAACTTC AGAGAAGTTT GGCAGCACTT GTACACTCCA AAAAATCACC TAATGTCTGC 
TTGTCACTGC TTCAGGCTGA ACAGGGTAAA GTGCATCTGG GTGCTGTGCT TCAACTGCTC 
AGAACTGGAG ACAAAGGGAA AGCTGCCATC TTGTGGGAGG AGATAGAAGC AGGATTAGAG 
GCAGTGACAC CTAAAGGAGC ACTGACACCA GAACTTGGGC CTATCAGAGC TACTCTACTG 
GGGGCTAAAG CCGTAGCCTG CTGCTTGGCG TTGGCAATGA AGAAACAGTG TATACCCGAA 
GAGCTGTTTT CTTCATTGTG GCACTGGAAT CCACCAAAGG TTAAACCGCA ATTAAAATCT 
AAGCATCGAT CCAAGAGTCC GGACGCAGCT CCTCTAGAGG CAGGTGTTAA ACATAACCCT 
AAAGTTAAGA GTGGACTTGA GGAGAATGAA AGCAGAAAAA CCAAAGAGAC CACTGTCATT 
TCGAAGAAAG CTAAAGACTC TGTGCCCAAA ATTACCATCA CAAAATCCTC CATGGTTTTT 
AAAACGCCCA AAGCAACGAG AACAACCCGG CCCAAATCTG TCTCCACTAG CACTGGCATC 
AGTGACCTGA GAGCATTTGA CTTCAATAAT GAGGTGCCTG ACATTTCAGT CAACTTCACA 
CCATCATTAC ACCGATCTAC AAACCAAAGT GCGACTGCAA AGTCAAAAGC GTCCTCAAAG 
GGTTCGTTCC AGGTTTACAA AGATTCTTCT CCTGCTGCAG AGAAAGCTGT AGTTGTCCCG 
GCTGCTCCTA AACGAACAAA GCGTTCTCGC TTCAAGGTTG AGTTCAGCGA TGAAAGCGAC 
AACGATGCTG CTCCTCTTGC TGTAATGGAA AAGTCAGAGA AAAAGAAAAA CACCTTTAAA 
ACATCACGCA CCTTGAAACC AACCCTTAAC ACCATCCCTA GTGACACCAA ACTTGTGAGC 
TCCACAGTAG ATCCAGAACC CTCCAGACGA ACACGGACCA CTAAGAAAAG CACAGCTCTT 
CCCTCCATTG GTGGCCTCTC TTCTGAGGAA GAACTTGGAG TATCTTCACA GACACGTACG 
CGTAGAGGAC GACCAAAGAA GAGCACCAGC TCAGCAGACA CAGAGGAGCC AGAGAGGATG 
AGGATGATTA AGGAGGAAGA ACAGAGGGTT CTTTTGAATG TCAGCATGGA GGAGCTCAGA 
GGCTCTGACA CTGAGACAAA AGACACTGGA AGTCCAGATG CTGACTGTGA GGTCTTGAGG 
AGAGATCTAG GTGCAGATTT TGGGCGAGAG TGTTTCAGTG AGCTCAGGAG AGCTGGACAG 
AATGGATCAG GATCTCACAC AGCTCTGCCA CATGCAACTA CATCTCCTGC AGACTTGTCA 
GTAGAGACGG TCCAGTCATA CCTGCGCTCC TCATGGCTCC TCCTGCACCA CTTCCCGTTT 
CCTTCTCTTT ATCCTCACAT CTGCTCATTA CTCGCTCAGT CATTCGGTCA AACGGACCCC 
ATCATCACAG CAATGCTGCA TGCACAGGCA CTGGGAGTGT CCACTCGCCA CCATATGACC 
CGTCACGTGG TTAACCAGTT CAGAAAGTTG AAGAAATCCT GTAACGATGT AGCAGAAGGT 
CTTGGAGCTC TGAGTCTGGA GGAAACATCT GGAGCAACGC AGTCAAAGAA ACTCTCTGCG 
TTGGAGCAGA TCTTTTCATT CACAGCATCA AACCCAATGC AGTTTCCACA GACACACTGT 
CAGCAGTTCA CACAGCAGCT GGAAGATCTA CCTGCAGGAG TGACCGTATG CTTGCTGTCA 
CTGACGGGGG TGCATCCTGA TGAAATTGGC AACACTATTC TTCTGACCCG ACTGGAGCGC 
GGCTCTACCC CAATCACAGT TCGAATCCCC ACTGCAGACA GAGAGCAATC TGTTGCTGGC 
CTGTTGGAAG AAATGGATGG AGTCTTGAAA GGACAAAAGG AAGTGAGCAC AGTGGCTGAG 
AAATCACAGT GGTGGGACGG AAGGAAGGCT TTAGATGCCC GTGTGGAGAA AATGCTGGAG 
GAGATGGAAG AGGCTTTAGG AGTGTGGCGA ACTCTTTTTC TGCCTTTGAC CTCTGATCCT 
AAGCTTGATG TTCAGGTAAA GAGCCTCCAA AAGGCTCTTA AATCAACAAA GATCACACGG 
GACACTCTCA AGGTGATTCT ATCAGCGTCT CCACTGCTGT CCCTGCCAGA CCTGCAGTGT 
CTTCTGGAAG GAATGAATGT GCAGGACAAG GACCTGCTGA AGCTCCTGCA GAGAGGCGTG 
GCTGAACTGA GAGGGAGGGA GGAGCCACAG GGACACACTG TTCTGATTTT AGACAAGTAC 
TTGCAGAGGT TGCCGTGGGA GAACATTGCT TGTCTGAAGT CTCAGTCGGT CACTCGAATG 
CCCTCATTAC ACGCGGTGCT TGGACACAGT CATCTGAGAG AGGTGGATAC AAGTTGTGTT 
CTATCTTGTG GTGTGAACCC TAAGCAGGTT TACTATGTGC TAAACCCAGA TGGAAACCTG 
CCCGACACTG AGAATCGCTT CAAAGAGTGG TTTACAGGTG AACGAACATG GCAGGGGGTT 
TGTGGAAGGG CTCCTGATCC GGAGAAACTA CAAGAGGCTG TGACCACTAA AGATCTCTAC 
ATATACATCG GACATGGTGC AGGAGCCCGC TTTCTGGATG CGCAGAGAGT TTTGAAGGTG 
CCTGTGAGGA CCGCAGCTCT TCTGTTCGGC TGCAGTAGTG CTGCTCTCAG TGTGCTTGGT 
CAGCAGGAGG GAACGGGCAT CATCCTCAGC TACCTGACCG CTGGATGTCC ATTGGTGCTG 
GGTAACCTGT GGGATGTGAC AGATCGTGAC TTGGATCGAT TCACATCGGC TCTGCTCCAG 
TCTTGGCTCT CTGCTGGTTC TGGCTCTTCA CTTCTTCAGC ATTTGGCGAA ATCACGCAGC 
GCAACTCATT TAAAACACAT AATCGGTGCT GCGCCCATTG CGTATGGTTT ACCGGTGCAT 
ATACACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

espl1 ( XM_001337833.5 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_001337869.1 Danio rerio extra spindle pole bodies like 1, separase (espl1), transcript variant X1, mRNA.

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CloneID	ODa12112
Clone ID Related Accession (Same CDS sequence)	XM_021477220.1 , XM_001337833.4 , XM_001337833.5 , XM_017356662.1
Accession Version	XM_001337833.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6549bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	separin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395382.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_001337833.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCATTAA AGAGCTTGAA AACAGAAGAT TACGTTCAGC AGTTATCCTG TGTCAAGGAC 
ACCATAGTCC TCCACAACGA ACTGAAGAAA CATGTGGATG GTGGCTTGGG TCCTTATGGT 
CGCACAATGT GTGATCGTAT CATCAGAGCC TGCAACCAGC GGCTGGGCAA CGGCGCTGTG 
GATGCTGGCC TTCAGGAGCG GATAGTGGAG CTGGTGGAAC TGGCTGTCCA AGGGTTTCAA 
CTGCTTGAAG AGTCCGGCAT GCAGAGCACC CCCTTTTATC TGGAGAAAAT AGTCTTTCAC 
ATCCTACAGA AAGTCGCAAG CCTTGGAGCT CATGGTCCTG CTTGTCGACT GGGTCAGCTC 
GTGTATAGAA GACTGAAATG TTTGTCTGCA GAGATTGATG ACTTTAATGT CCTGGTGCGG 
AATTGCTTTG CAGTCTTGTG GAACAGTCTC CTCTCTAACA AACCGAGTGC TTCCACCCTC 
CCTCCACGAG ACAGACTCCA CTGTCAGCTG CAAGCTTTAA CATTCAGACT CCTTGAACAG 
GGCTCCAGCT CGTCCTCTTC CCCATCCAAA GTTCCTCTGT TCGTGGAGGA AGCAGTAGTG 
GAGTACGAAC GATCATGTGG AGGTTTTACT CAAGACGATG TGAGTTTTCT GACCTCTGAA 
TTGCGTGAGC TGTTCTCTGG TGCTTTCATC AATCCTCAGG ACTCTGCATC AACTTCTTCA 
CTGCTGGCTG TGTGGTGTGA GGTGGCATTT AAAGTGTCTA AACTGCTCTG CAAAAGTAGC 
TTTTCACCTG AGGCTGTTGA ACTCTTGCGA GGCACTCTGA AGGAGGTTAA TGGCCACGTT 
GGCCTGCGTT CGGCTCTGGG TCTGGCAGAC CGGGCAGTGC AGATGCAGTG TGTGTTAAGT 
TCTGGAGGTG AATGCAGTAA GAGCTTCACT GAATGTGCCC GTGCTCTCCG GATGTTGCCT 
TGTGCCATGT CCAGCTCAGA GTCTCATGCC CTGTTAGAGG CTTGTCAGCT GGTGGTTTGG 
ACGTTGGAAG CCAGTCAGTG TAAAGGAATG GATGTGACCA CTTTGCTGGC TTGTTTTTCC 
TTTTTGGAAG AATATCAGGA ATTTCTGCTT GCACAGCAAA AGAACTCGTT TTCGCTGCAG 
GTCCAATACT CTCTTTGTTT CAGTTTTTAC CAGGGCTTCA TCTGCACATA TGATAGTCTT 
CATGCTTCTC AGGTGTCTGT GGGCGAGACC TTGGACAGAG TGCTATTGTT CTGCCGGTCG 
ACCGCAGGAC GTATGCTTGC TGAACTGCGG ACACTCAATA ATGACAACTT GCTGCTTAAA 
GCAGTGTGTG CAGTGAATAA TGTGGTGTAC GAGCTGTTTA ACAGGAAGCT GTATGAAGGA 
GCCTTTGGAT TGTCGATGAT TGTGTGTCAG GAGCTCTGTA AGGACTGCCC GCCCTCTCTT 
CCTATGGATC GGGTCAACCG CTGTTTCATG CTGACAGTGC AGTGTAGTCG CCGCGCAGGC 
CATCTGGAGC AGGCTCTGGA CTGGATTGTT CGCTGGATTC AGGCTTTGGG CTCACAGATT 
TTGGATCATC TCACTGAACC TGTGTCGATG TGGGTCAAAA CTAAGTGTGA TGCCGCCAGA 
GCAGGCGAGG ATGACACACG CCTGAGGACT CTGAGGGACG GGTTAGGAGA ATCCTTAGTG 
GATGAGGAGG TTATGATGTG TTTGTTAGAG GAGGAGTTGC GTGTATATAA GGAGCAGACT 
GGAGACACAG CACAGGAACG CTACAACACA CTCTGCGACC TCTTGGAAAT CTGCCATGAA 
GAAACCCCAC ACACACTAAG AAGAGCTACG TATCTGTGTG AGATGGCACA GGTTGTGTGC 
TATCAGGACT TCAGCCAGCA GACTGACTGC TCAGCAGTTG ATTTTACCCA CGAGGCTTTG 
CGGTTGCTGG AAGCAGAACC AGAGACGGCA GAGAATACTG ATAGGCTGAA GGATGAGAAA 
GCCCACGCTT CCCTGTGGCT CTATATCTGC ACTCTTGAGT CTAACCTGCA GGAGGCTGTG 
GACACAGAGA AGCGTTTGTG TGCAGTGCAG GAGGAGAATA AAAGAGATGT GAATATGGAT 
CCTGTTCCCA CAAATGATCT GGAGTACGAG GACAAACAGA AATCACAAGA GAGCCAGTTA 
GTCTACGATG GACTACGCTT CAACCTGCTG GCTCACAGCA AGCAAAGTGA ACCTCTAGAT 
AAATGTTTGT CTCTATGGCG GAGTCTCCTC AAGGGGGCTG TGCCTGCAGT TAGAGACCCT 
AAACTCACAG CTTCTTCCAT GACTTTGATG GCTGCCCTAT ACACACTTAT GGGCAAGCAC 
CTGCAAGCTC TGGAGGGATA TCAGCTTGCT GCCACCCTTT TCCATGTACT TGGTGATGGC 
CAGAGTAGTG CCAATGCCTT CTGCCACTCT ACCAGGATCT TGCTTTACCT GGGTTCACCG 
CAGCTTGCAC AGGTGGACTT AGAAAAAGCA GAAAAGAGCT TGTCATCTGT CACCATCTCT 
GAGGGCACAT CTATTATCTC CATGCTGGCC ATGTTGCAAA AAGCACAGCT ACACTATGCT 
CTAGGACAGG TGGAGAGTGG CGTGTCCTGC CTGATGGAGG TGATAAAGGA ATCTGCTCAG 
CATCATTCTA AGAGCTGGTA CCTGCTCAGG GCTCGAGCAC TGCAGACAGC CAGTGAATAC 
TTGAGTCTGG ACACACTGGC TTTGAACTCA CAGCTACGCC AGAGCATCAT GCAGCAGGGT 
CTAAAGACTC CAGACACTGC TCAATATGAA GGGTTGAAGC TGTTGTGTAG CCTGGTGATG 
ATGTTATTGG GCAACGGGTT TTACGGAGCG CCTGGACCAA ACACTGACAC TCGCTTTGTG 
GATAAAGGAG ACAGTGTTGT GTTTAAGTGG CTGCTGCTGA GTGAAGTGCT GGTGTGCTCG 
GAGAGAATGG TGATCACCAG GAGCAGCAGC GGAGCCGTTC ATGAAGCTAA AGCTCAGTGT 
CTAGAGGCTC TCAAACTGGC CACCAAACTA CAGACACTCA GCCACTGTGC TGAGCTGTTG 
GTGCTGAAGG CAGAGCTGGA GCTGATGAAG GGTGCAACTG AAGCTAGTAG TTTAGACCTA 
GAGCAAGTTA GACATTTACT TGATCTCTGC ACAGATTTTG GCCAGGGACG ACAACAGAAA 
TCTGAAGTGA AAATCAAACC ACGCAAAGGT CGTCCCGCTG CCCCTTCTTC CTCTGGTCAC 
ATCTCTGAGG AAGAGGATGA TCTGAGTGGG ATCCTCAGCT CTCGTGCCCT TCCGAAGGAG 
CCTGTGGAGA ACATGGCTAG ATTAGGAAGC CAAGGAGCTT CTCCCCCTCT AAAGCCCAAA 
TGCCAGCATG GCCTCTCCTG TCTGACCCAT GAAGAGACCT GCTCTTGTCT CTGCTGCACT 
GAGCTCAGCG TGGCTCGAGT CAGCATCCAC TGGGCACTAT TACAGGCAGA TCTCCAGACA 
GATCCCGAAC GCTCTCTCAG ACTACGCCAG TCTGCCAGGA AACGCTGCCG CTATGTTGTA 
ACTAAACTTC AGAGAAGTTT GGCAGCACTT GTACACTCCA AAAAATCACC TAATGTCTGC 
TTGTCACTGC TTCAGGCTGA ACAGGGTAAA GTGCATCTGG GTGCTGTGCT TCAACTGCTC 
AGAACTGGAG ACAAAGGGAA AGCTGCCATC TTGTGGGAGG AGATAGAAGC AGGATTAGAG 
GCAGTGACAC CTAAAGGAGC ACTGACACCA GAACTTGGGC CTATCAGAGC TACTCTACTG 
GGGGCTAAAG CCGTAGCCTG CTGCTTGGCG TTGGCAATGA AGAAACAGTG TATACCCGAA 
GAGCTGTTTT CTTCATTGTG GCACTGGAAT CCACCAAAGG TTAAACCGCA ATTAAAATCT 
AAGCATCGAT CCAAGAGTCC GGACGCAGCT CCTCTAGAGG CAGGTGTTAA ACATAACCCT 
AAAGTTAAGA GTGGACTTGA GGAGAATGAA AGCAGAAAAA CCAAAGAGAC CACTGTCATT 
TCGAAGAAAG CTAAAGACTC TGTGCCCAAA ATTACCATCA CAAAATCCTC CATGGTTTTT 
AAAACGCCCA AAGCAACGAG AACAACCCGG CCCAAATCTG TCTCCACTAG CACTGGCATC 
AGTGACCTGA GAGCATTTGA CTTCAATAAT GAGGTGCCTG ACATTTCAGT CAACTTCACA 
CCATCATTAC ACCGATCTAC AAACCAAAGT GCGACTGCAA AGTCAAAAGC GTCCTCAAAG 
GGTTCGTTCC AGGTTTACAA AGATTCTTCT CCTGCTGCAG AGAAAGCTGT AGTTGTCCCG 
GCTGCTCCTA AACGAACAAA GCGTTCTCGC TTCAAGGTTG AGTTCAGCGA TGAAAGCGAC 
AACGATGCTG CTCCTCTTGC TGTAATGGAA AAGTCAGAGA AAAAGAAAAA CACCTTTAAA 
ACATCACGCA CCTTGAAACC AACCCTTAAC ACCATCCCTA GTGACACCAA ACTTGTGAGC 
TCCACAGTAG ATCCAGAACC CTCCAGACGA ACACGGACCA CTAAGAAAAG CACAGCTCTT 
CCCTCCATTG GTGGCCTCTC TTCTGAGGAA GAACTTGGAG TATCTTCACA GACACGTACG 
CGTAGAGGAC GACCAAAGAA GAGCACCAGC TCAGCAGACA CAGAGGAGCC AGAGAGGATG 
AGGATGATTA AGGAGGAAGA ACAGAGGGTT CTTTTGAATG TCAGCATGGA GGAGCTCAGA 
GGCTCTGACA CTGAGACAAA AGACACTGGA AGTCCAGATG CTGACTGTGA GGTCTTGAGG 
AGAGATCTAG GTGCAGATTT TGGGCGAGAG TGTTTCAGTG AGCTCAGGAG AGCTGGACAG 
AATGGATCAG GATCTCACAC AGCTCTGCCA CATGCAACTA CATCTCCTGC AGACTTGTCA 
GTAGAGACGG TCCAGTCATA CCTGCGCTCC TCATGGCTCC TCCTGCACCA CTTCCCGTTT 
CCTTCTCTTT ATCCTCACAT CTGCTCATTA CTCGCTCAGT CATTCGGTCA AACGGACCCC 
ATCATCACAG CAATGCTGCA TGCACAGGCA CTGGGAGTGT CCACTCGCCA CCATATGACC 
CGTCACGTGG TTAACCAGTT CAGAAAGTTG AAGAAATCCT GTAACGATGT AGCAGAAGGT 
CTTGGAGCTC TGAGTCTGGA GGAAACATCT GGAGCAACGC AGTCAAAGAA ACTCTCTGCG 
TTGGAGCAGA TCTTTTCATT CACAGCATCA AACCCAATGC AGTTTCCACA GACACACTGT 
CAGCAGTTCA CACAGCAGCT GGAAGATCTA CCTGCAGGAG TGACCGTATG CTTGCTGTCA 
CTGACGGGGG TGCATCCTGA TGAAATTGGC AACACTATTC TTCTGACCCG ACTGGAGCGC 
GGCTCTACCC CAATCACAGT TCGAATCCCC ACTGCAGACA GAGAGCAATC TGTTGCTGGC 
CTGTTGGAAG AAATGGATGG AGTCTTGAAA GGACAAAAGG AAGTGAGCAC AGTGGCTGAG 
AAATCACAGT GGTGGGACGG AAGGAAGGCT TTAGATGCCC GTGTGGAGAA AATGCTGGAG 
GAGATGGAAG AGGCTTTAGG AGTGTGGCGA ACTCTTTTTC TGCCTTTGAC CTCTGATCCT 
AAGCTTGATG TTCAGGTAAA GAGCCTCCAA AAGGCTCTTA AATCAACAAA GATCACACGG 
GACACTCTCA AGGTGATTCT ATCAGCGTCT CCACTGCTGT CCCTGCCAGA CCTGCAGTGT 
CTTCTGGAAG GAATGAATGT GCAGGACAAG GACCTGCTGA AGCTCCTGCA GAGAGGCGTG 
GCTGAACTGA GAGGGAGGGA GGAGCCACAG GGACACACTG TTCTGATTTT AGACAAGTAC 
TTGCAGAGGT TGCCGTGGGA GAACATTGCT TGTCTGAAGT CTCAGTCGGT CACTCGAATG 
CCCTCATTAC ACGCGGTGCT TGGACACAGT CATCTGAGAG AGGTGGATAC AAGTTGTGTT 
CTATCTTGTG GTGTGAACCC TAAGCAGGTT TACTATGTGC TAAACCCAGA TGGAAACCTG 
CCCGACACTG AGAATCGCTT CAAAGAGTGG TTTACAGGTG AACGAACATG GCAGGGGGTT 
TGTGGAAGGG CTCCTGATCC GGAGAAACTA CAAGAGGCTG TGACCACTAA AGATCTCTAC 
ATATACATCG GACATGGTGC AGGAGCCCGC TTTCTGGATG CGCAGAGAGT TTTGAAGGTG 
CCTGTGAGGA CCGCAGCTCT TCTGTTCGGC TGCAGTAGTG CTGCTCTCAG TGTGCTTGGT 
CAGCAGGAGG GAACGGGCAT CATCCTCAGC TACCTGACCG CTGGATGTCC ATTGGTGCTG 
GGTAACCTGT GGGATGTGAC AGATCGTGAC TTGGATCGAT TCACATCGGC TCTGCTCCAG 
TCTTGGCTCT CTGCTGGTTC TGGCTCTTCA CTTCTTCAGC ATTTGGCGAA ATCACGCAGC 
GCAACTCATT TAAAACACAT AATCGGTGCT GCGCCCATTG CGTATGGTTT ACCGGTGCAT 
ATACACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001337869.1
CDS	210..6758
Translation	MALKSLKTEDYVQQLSCVKDTIVLHNELKKHVDGGLGPYGRTMCDRIIRACNQRLGNGAVDAGLQERIVELVELAVQGFQLLEESGMQSTPFYLEKIVFHILQKVASLGAHGPACRLGQLVYRRLKCLSAEIDDFNVLVRNCFAVLWNSLLSNKPSASTLPPRDRLHCQLQALTFRLLEQGSSSSSSPSKVPLFVEEAVVEYERSCGGFTQDDVSFLTSELRELFSGAFINPQDSASTSSLLAVWCEVAFKVSKLLCKSSFSPEAVELLRGTLKEVNGHVGLRSALGLADRAVQMQCVLSSGGECSKSFTECARALRMLPCAMSSSESHALLEACQLVVWTLEASQCKGMDVTTLLACFSFLEEYQEFLLAQQKNSFSLQVQYSLCFSFYQGFICTYDSLHASQVSVGETLDRVLLFCRSTAGRMLAELRTLNNDNLLLKAVCAVNNVVYELFNRKLYEGAFGLSMIVCQELCKDCPPSLPMDRVNRCFMLTVQCSRRAGHLEQALDWIVRWIQALGSQILDHLTEPVSMWVKTKCDAARAGEDDTRLRTLRDGLGESLVDEEVMMCLLEEELRVYKEQTGDTAQERYNTLCDLLEICHEETPHTLRRATYLCEMAQVVCYQDFSQQTDCSAVDFTHEALRLLEAEPETAENTDRLKDEKAHASLWLYICTLESNLQEAVDTEKRLCAVQEENKRDVNMDPVPTNDLEYEDKQKSQESQLVYDGLRFNLLAHSKQSEPLDKCLSLWRSLLKGAVPAVRDPKLTASSMTLMAALYTLMGKHLQALEGYQLAATLFHVLGDGQSSANAFCHSTRILLYLGSPQLAQVDLEKAEKSLSSVTISEGTSIISMLAMLQKAQLHYALGQVESGVSCLMEVIKESAQHHSKSWYLLRARALQTASEYLSLDTLALNSQLRQSIMQQGLKTPDTAQYEGLKLLCSLVMMLLGNGFYGAPGPNTDTRFVDKGDSVVFKWLLLSEVLVCSERMVITRSSSGAVHEAKAQCLEALKLATKLQTLSHCAELLVLKAELELMKGATEASSLDLEQVRHLLDLCTDFGQGRQQKSEVKIKPRKGRPAAPSSSGHISEEEDDLSGILSSRALPKEPVENMARLGSQGASPPLKPKCQHGLSCLTHEETCSCLCCTELSVARVSIHWALLQADLQTDPERSLRLRQSARKRCRYVVTKLQRSLAALVHSKKSPNVCLSLLQAEQGKVHLGAVLQLLRTGDKGKAAILWEEIEAGLEAVTPKGALTPELGPIRATLLGAKAVACCLALAMKKQCIPEELFSSLWHWNPPKVKPQLKSKHRSKSPDAAPLEAGVKHNPKVKSGLEENESRKTKETTVISKKAKDSVPKITITKSSMVFKTPKATRTTRPKSVSTSTGISDLRAFDFNNEVPDISVNFTPSLHRSTNQSATAKSKASSKGSFQVYKDSSPAAEKAVVVPAAPKRTKRSRFKVEFSDESDNDAAPLAVMEKSEKKKNTFKTSRTLKPTLNTIPSDTKLVSSTVDPEPSRRTRTTKKSTALPSIGGLSSEEELGVSSQTRTRRGRPKKSTSSADTEEPERMRMIKEEEQRVLLNVSMEELRGSDTETKDTGSPDADCEVLRRDLGADFGRECFSELRRAGQNGSGSHTALPHATTSPADLSVETVQSYLRSSWLLLHHFPFPSLYPHICSLLAQSFGQTDPIITAMLHAQALGVSTRHHMTRHVVNQFRKLKKSCNDVAEGLGALSLEETSGATQSKKLSALEQIFSFTASNPMQFPQTHCQQFTQQLEDLPAGVTVCLLSLTGVHPDEIGNTILLTRLERGSTPITVRIPTADREQSVAGLLEEMDGVLKGQKEVSTVAEKSQWWDGRKALDARVEKMLEEMEEALGVWRTLFLPLTSDPKLDVQVKSLQKALKSTKITRDTLKVILSASPLLSLPDLQCLLEGMNVQDKDLLKLLQRGVAELRGREEPQGHTVLILDKYLQRLPWENIACLKSQSVTRMPSLHAVLGHSHLREVDTSCVLSCGVNPKQVYYVLNPDGNLPDTENRFKEWFTGERTWQGVCGRAPDPEKLQEAVTTKDLYIYIGHGAGARFLDAQRVLKVPVRTAALLFGCSSAALSVLGQQEGTGIILSYLTAGCPLVLGNLWDVTDRDLDRFTSALLQSWLSAGSGSSLLQHLAKSRSATHLKHIIGAAPIAYGLPVHIH

Target ORF information:

RefSeq Version	XM_001337833.5
Organism	Danio rerio(zebrafish)
Definition	Danio rerio extra spindle pole bodies like 1, separase (espl1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001337833.5

ORF Insert Sequence:

ATGGCATTAA AGAGCTTGAA AACAGAAGAT TACGTTCAGC AGTTATCCTG TGTCAAGGAC 
ACCATAGTCC TCCACAACGA ACTGAAGAAA CATGTGGATG GTGGCTTGGG TCCTTATGGT 
CGCACAATGT GTGATCGTAT CATCAGAGCC TGCAACCAGC GGCTGGGCAA CGGCGCTGTG 
GATGCTGGCC TTCAGGAGCG GATAGTGGAG CTGGTGGAAC TGGCTGTCCA AGGGTTTCAA 
CTGCTTGAAG AGTCCGGCAT GCAGAGCACC CCCTTTTATC TGGAGAAAAT AGTCTTTCAC 
ATCCTACAGA AAGTCGCAAG CCTTGGAGCT CATGGTCCTG CTTGTCGACT GGGTCAGCTC 
GTGTATAGAA GACTGAAATG TTTGTCTGCA GAGATTGATG ACTTTAATGT CCTGGTGCGG 
AATTGCTTTG CAGTCTTGTG GAACAGTCTC CTCTCTAACA AACCGAGTGC TTCCACCCTC 
CCTCCACGAG ACAGACTCCA CTGTCAGCTG CAAGCTTTAA CATTCAGACT CCTTGAACAG 
GGCTCCAGCT CGTCCTCTTC CCCATCCAAA GTTCCTCTGT TCGTGGAGGA AGCAGTAGTG 
GAGTACGAAC GATCATGTGG AGGTTTTACT CAAGACGATG TGAGTTTTCT GACCTCTGAA 
TTGCGTGAGC TGTTCTCTGG TGCTTTCATC AATCCTCAGG ACTCTGCATC AACTTCTTCA 
CTGCTGGCTG TGTGGTGTGA GGTGGCATTT AAAGTGTCTA AACTGCTCTG CAAAAGTAGC 
TTTTCACCTG AGGCTGTTGA ACTCTTGCGA GGCACTCTGA AGGAGGTTAA TGGCCACGTT 
GGCCTGCGTT CGGCTCTGGG TCTGGCAGAC CGGGCAGTGC AGATGCAGTG TGTGTTAAGT 
TCTGGAGGTG AATGCAGTAA GAGCTTCACT GAATGTGCCC GTGCTCTCCG GATGTTGCCT 
TGTGCCATGT CCAGCTCAGA GTCTCATGCC CTGTTAGAGG CTTGTCAGCT GGTGGTTTGG 
ACGTTGGAAG CCAGTCAGTG TAAAGGAATG GATGTGACCA CTTTGCTGGC TTGTTTTTCC 
TTTTTGGAAG AATATCAGGA ATTTCTGCTT GCACAGCAAA AGAACTCGTT TTCGCTGCAG 
GTCCAATACT CTCTTTGTTT CAGTTTTTAC CAGGGCTTCA TCTGCACATA TGATAGTCTT 
CATGCTTCTC AGGTGTCTGT GGGCGAGACC TTGGACAGAG TGCTATTGTT CTGCCGGTCG 
ACCGCAGGAC GTATGCTTGC TGAACTGCGG ACACTCAATA ATGACAACTT GCTGCTTAAA 
GCAGTGTGTG CAGTGAATAA TGTGGTGTAC GAGCTGTTTA ACAGGAAGCT GTATGAAGGA 
GCCTTTGGAT TGTCGATGAT TGTGTGTCAG GAGCTCTGTA AGGACTGCCC GCCCTCTCTT 
CCTATGGATC GGGTCAACCG CTGTTTCATG CTGACAGTGC AGTGTAGTCG CCGCGCAGGC 
CATCTGGAGC AGGCTCTGGA CTGGATTGTT CGCTGGATTC AGGCTTTGGG CTCACAGATT 
TTGGATCATC TCACTGAACC TGTGTCGATG TGGGTCAAAA CTAAGTGTGA TGCCGCCAGA 
GCAGGCGAGG ATGACACACG CCTGAGGACT CTGAGGGACG GGTTAGGAGA ATCCTTAGTG 
GATGAGGAGG TTATGATGTG TTTGTTAGAG GAGGAGTTGC GTGTATATAA GGAGCAGACT 
GGAGACACAG CACAGGAACG CTACAACACA CTCTGCGACC TCTTGGAAAT CTGCCATGAA 
GAAACCCCAC ACACACTAAG AAGAGCTACG TATCTGTGTG AGATGGCACA GGTTGTGTGC 
TATCAGGACT TCAGCCAGCA GACTGACTGC TCAGCAGTTG ATTTTACCCA CGAGGCTTTG 
CGGTTGCTGG AAGCAGAACC AGAGACGGCA GAGAATACTG ATAGGCTGAA GGATGAGAAA 
GCCCACGCTT CCCTGTGGCT CTATATCTGC ACTCTTGAGT CTAACCTGCA GGAGGCTGTG 
GACACAGAGA AGCGTTTGTG TGCAGTGCAG GAGGAGAATA AAAGAGATGT GAATATGGAT 
CCTGTTCCCA CAAATGATCT GGAGTACGAG GACAAACAGA AATCACAAGA GAGCCAGTTA 
GTCTACGATG GACTACGCTT CAACCTGCTG GCTCACAGCA AGCAAAGTGA ACCTCTAGAT 
AAATGTTTGT CTCTATGGCG GAGTCTCCTC AAGGGGGCTG TGCCTGCAGT TAGAGACCCT 
AAACTCACAG CTTCTTCCAT GACTTTGATG GCTGCCCTAT ACACACTTAT GGGCAAGCAC 
CTGCAAGCTC TGGAGGGATA TCAGCTTGCT GCCACCCTTT TCCATGTACT TGGTGATGGC 
CAGAGTAGTG CCAATGCCTT CTGCCACTCT ACCAGGATCT TGCTTTACCT GGGTTCACCG 
CAGCTTGCAC AGGTGGACTT AGAAAAAGCA GAAAAGAGCT TGTCATCTGT CACCATCTCT 
GAGGGCACAT CTATTATCTC CATGCTGGCC ATGTTGCAAA AAGCACAGCT ACACTATGCT 
CTAGGACAGG TGGAGAGTGG CGTGTCCTGC CTGATGGAGG TGATAAAGGA ATCTGCTCAG 
CATCATTCTA AGAGCTGGTA CCTGCTCAGG GCTCGAGCAC TGCAGACAGC CAGTGAATAC 
TTGAGTCTGG ACACACTGGC TTTGAACTCA CAGCTACGCC AGAGCATCAT GCAGCAGGGT 
CTAAAGACTC CAGACACTGC TCAATATGAA GGGTTGAAGC TGTTGTGTAG CCTGGTGATG 
ATGTTATTGG GCAACGGGTT TTACGGAGCG CCTGGACCAA ACACTGACAC TCGCTTTGTG 
GATAAAGGAG ACAGTGTTGT GTTTAAGTGG CTGCTGCTGA GTGAAGTGCT GGTGTGCTCG 
GAGAGAATGG TGATCACCAG GAGCAGCAGC GGAGCCGTTC ATGAAGCTAA AGCTCAGTGT 
CTAGAGGCTC TCAAACTGGC CACCAAACTA CAGACACTCA GCCACTGTGC TGAGCTGTTG 
GTGCTGAAGG CAGAGCTGGA GCTGATGAAG GGTGCAACTG AAGCTAGTAG TTTAGACCTA 
GAGCAAGTTA GACATTTACT TGATCTCTGC ACAGATTTTG GCCAGGGACG ACAACAGAAA 
TCTGAAGTGA AAATCAAACC ACGCAAAGGT CGTCCCGCTG CCCCTTCTTC CTCTGGTCAC 
ATCTCTGAGG AAGAGGATGA TCTGAGTGGG ATCCTCAGCT CTCGTGCCCT TCCGAAGGAG 
CCTGTGGAGA ACATGGCTAG ATTAGGAAGC CAAGGAGCTT CTCCCCCTCT AAAGCCCAAA 
TGCCAGCATG GCCTCTCCTG TCTGACCCAT GAAGAGACCT GCTCTTGTCT CTGCTGCACT 
GAGCTCAGCG TGGCTCGAGT CAGCATCCAC TGGGCACTAT TACAGGCAGA TCTCCAGACA 
GATCCCGAAC GCTCTCTCAG ACTACGCCAG TCTGCCAGGA AACGCTGCCG CTATGTTGTA 
ACTAAACTTC AGAGAAGTTT GGCAGCACTT GTACACTCCA AAAAATCACC TAATGTCTGC 
TTGTCACTGC TTCAGGCTGA ACAGGGTAAA GTGCATCTGG GTGCTGTGCT TCAACTGCTC 
AGAACTGGAG ACAAAGGGAA AGCTGCCATC TTGTGGGAGG AGATAGAAGC AGGATTAGAG 
GCAGTGACAC CTAAAGGAGC ACTGACACCA GAACTTGGGC CTATCAGAGC TACTCTACTG 
GGGGCTAAAG CCGTAGCCTG CTGCTTGGCG TTGGCAATGA AGAAACAGTG TATACCCGAA 
GAGCTGTTTT CTTCATTGTG GCACTGGAAT CCACCAAAGG TTAAACCGCA ATTAAAATCT 
AAGCATCGAT CCAAGAGTCC GGACGCAGCT CCTCTAGAGG CAGGTGTTAA ACATAACCCT 
AAAGTTAAGA GTGGACTTGA GGAGAATGAA AGCAGAAAAA CCAAAGAGAC CACTGTCATT 
TCGAAGAAAG CTAAAGACTC TGTGCCCAAA ATTACCATCA CAAAATCCTC CATGGTTTTT 
AAAACGCCCA AAGCAACGAG AACAACCCGG CCCAAATCTG TCTCCACTAG CACTGGCATC 
AGTGACCTGA GAGCATTTGA CTTCAATAAT GAGGTGCCTG ACATTTCAGT CAACTTCACA 
CCATCATTAC ACCGATCTAC AAACCAAAGT GCGACTGCAA AGTCAAAAGC GTCCTCAAAG 
GGTTCGTTCC AGGTTTACAA AGATTCTTCT CCTGCTGCAG AGAAAGCTGT AGTTGTCCCG 
GCTGCTCCTA AACGAACAAA GCGTTCTCGC TTCAAGGTTG AGTTCAGCGA TGAAAGCGAC 
AACGATGCTG CTCCTCTTGC TGTAATGGAA AAGTCAGAGA AAAAGAAAAA CACCTTTAAA 
ACATCACGCA CCTTGAAACC AACCCTTAAC ACCATCCCTA GTGACACCAA ACTTGTGAGC 
TCCACAGTAG ATCCAGAACC CTCCAGACGA ACACGGACCA CTAAGAAAAG CACAGCTCTT 
CCCTCCATTG GTGGCCTCTC TTCTGAGGAA GAACTTGGAG TATCTTCACA GACACGTACG 
CGTAGAGGAC GACCAAAGAA GAGCACCAGC TCAGCAGACA CAGAGGAGCC AGAGAGGATG 
AGGATGATTA AGGAGGAAGA ACAGAGGGTT CTTTTGAATG TCAGCATGGA GGAGCTCAGA 
GGCTCTGACA CTGAGACAAA AGACACTGGA AGTCCAGATG CTGACTGTGA GGTCTTGAGG 
AGAGATCTAG GTGCAGATTT TGGGCGAGAG TGTTTCAGTG AGCTCAGGAG AGCTGGACAG 
AATGGATCAG GATCTCACAC AGCTCTGCCA CATGCAACTA CATCTCCTGC AGACTTGTCA 
GTAGAGACGG TCCAGTCATA CCTGCGCTCC TCATGGCTCC TCCTGCACCA CTTCCCGTTT 
CCTTCTCTTT ATCCTCACAT CTGCTCATTA CTCGCTCAGT CATTCGGTCA AACGGACCCC 
ATCATCACAG CAATGCTGCA TGCACAGGCA CTGGGAGTGT CCACTCGCCA CCATATGACC 
CGTCACGTGG TTAACCAGTT CAGAAAGTTG AAGAAATCCT GTAACGATGT AGCAGAAGGT 
CTTGGAGCTC TGAGTCTGGA GGAAACATCT GGAGCAACGC AGTCAAAGAA ACTCTCTGCG 
TTGGAGCAGA TCTTTTCATT CACAGCATCA AACCCAATGC AGTTTCCACA GACACACTGT 
CAGCAGTTCA CACAGCAGCT GGAAGATCTA CCTGCAGGAG TGACCGTATG CTTGCTGTCA 
CTGACGGGGG TGCATCCTGA TGAAATTGGC AACACTATTC TTCTGACCCG ACTGGAGCGC 
GGCTCTACCC CAATCACAGT TCGAATCCCC ACTGCAGACA GAGAGCAATC TGTTGCTGGC 
CTGTTGGAAG AAATGGATGG AGTCTTGAAA GGACAAAAGG AAGTGAGCAC AGTGGCTGAG 
AAATCACAGT GGTGGGACGG AAGGAAGGCT TTAGATGCCC GTGTGGAGAA AATGCTGGAG 
GAGATGGAAG AGGCTTTAGG AGTGTGGCGA ACTCTTTTTC TGCCTTTGAC CTCTGATCCT 
AAGCTTGATG TTCAGGTAAA GAGCCTCCAA AAGGCTCTTA AATCAACAAA GATCACACGG 
GACACTCTCA AGGTGATTCT ATCAGCGTCT CCACTGCTGT CCCTGCCAGA CCTGCAGTGT 
CTTCTGGAAG GAATGAATGT GCAGGACAAG GACCTGCTGA AGCTCCTGCA GAGAGGCGTG 
GCTGAACTGA GAGGGAGGGA GGAGCCACAG GGACACACTG TTCTGATTTT AGACAAGTAC 
TTGCAGAGGT TGCCGTGGGA GAACATTGCT TGTCTGAAGT CTCAGTCGGT CACTCGAATG 
CCCTCATTAC ACGCGGTGCT TGGACACAGT CATCTGAGAG AGGTGGATAC AAGTTGTGTT 
CTATCTTGTG GTGTGAACCC TAAGCAGGTT TACTATGTGC TAAACCCAGA TGGAAACCTG 
CCCGACACTG AGAATCGCTT CAAAGAGTGG TTTACAGGTG AACGAACATG GCAGGGGGTT 
TGTGGAAGGG CTCCTGATCC GGAGAAACTA CAAGAGGCTG TGACCACTAA AGATCTCTAC 
ATATACATCG GACATGGTGC AGGAGCCCGC TTTCTGGATG CGCAGAGAGT TTTGAAGGTG 
CCTGTGAGGA CCGCAGCTCT TCTGTTCGGC TGCAGTAGTG CTGCTCTCAG TGTGCTTGGT 
CAGCAGGAGG GAACGGGCAT CATCCTCAGC TACCTGACCG CTGGATGTCC ATTGGTGCTG 
GGTAACCTGT GGGATGTGAC AGATCGTGAC TTGGATCGAT TCACATCGGC TCTGCTCCAG 
TCTTGGCTCT CTGCTGGTTC TGGCTCTTCA CTTCTTCAGC ATTTGGCGAA ATCACGCAGC 
GCAACTCATT TAAAACACAT AATCGGTGCT GCGCCCATTG CGTATGGTTT ACCGGTGCAT 
ATACACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

espl1 ( XM_017356662.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017212151.1 Danio rerio extra spindle pole bodies like 1, separase (espl1), transcript variant X2, mRNA.

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CloneID	ODa12112
Clone ID Related Accession (Same CDS sequence)	XM_021477220.1 , XM_001337833.4 , XM_001337833.5 , XM_017356662.1
Accession Version	XM_017356662.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6549bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	separin isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCATTAA AGAGCTTGAA AACAGAAGAT TACGTTCAGC AGTTATCCTG TGTCAAGGAC 
ACCATAGTCC TCCACAACGA ACTGAAGAAA CATGTGGATG GTGGCTTGGG TCCTTATGGT 
CGCACAATGT GTGATCGTAT CATCAGAGCC TGCAACCAGC GGCTGGGCAA CGGCGCTGTG 
GATGCTGGCC TTCAGGAGCG GATAGTGGAG CTGGTGGAAC TGGCTGTCCA AGGGTTTCAA 
CTGCTTGAAG AGTCCGGCAT GCAGAGCACC CCCTTTTATC TGGAGAAAAT AGTCTTTCAC 
ATCCTACAGA AAGTCGCAAG CCTTGGAGCT CATGGTCCTG CTTGTCGACT GGGTCAGCTC 
GTGTATAGAA GACTGAAATG TTTGTCTGCA GAGATTGATG ACTTTAATGT CCTGGTGCGG 
AATTGCTTTG CAGTCTTGTG GAACAGTCTC CTCTCTAACA AACCGAGTGC TTCCACCCTC 
CCTCCACGAG ACAGACTCCA CTGTCAGCTG CAAGCTTTAA CATTCAGACT CCTTGAACAG 
GGCTCCAGCT CGTCCTCTTC CCCATCCAAA GTTCCTCTGT TCGTGGAGGA AGCAGTAGTG 
GAGTACGAAC GATCATGTGG AGGTTTTACT CAAGACGATG TGAGTTTTCT GACCTCTGAA 
TTGCGTGAGC TGTTCTCTGG TGCTTTCATC AATCCTCAGG ACTCTGCATC AACTTCTTCA 
CTGCTGGCTG TGTGGTGTGA GGTGGCATTT AAAGTGTCTA AACTGCTCTG CAAAAGTAGC 
TTTTCACCTG AGGCTGTTGA ACTCTTGCGA GGCACTCTGA AGGAGGTTAA TGGCCACGTT 
GGCCTGCGTT CGGCTCTGGG TCTGGCAGAC CGGGCAGTGC AGATGCAGTG TGTGTTAAGT 
TCTGGAGGTG AATGCAGTAA GAGCTTCACT GAATGTGCCC GTGCTCTCCG GATGTTGCCT 
TGTGCCATGT CCAGCTCAGA GTCTCATGCC CTGTTAGAGG CTTGTCAGCT GGTGGTTTGG 
ACGTTGGAAG CCAGTCAGTG TAAAGGAATG GATGTGACCA CTTTGCTGGC TTGTTTTTCC 
TTTTTGGAAG AATATCAGGA ATTTCTGCTT GCACAGCAAA AGAACTCGTT TTCGCTGCAG 
GTCCAATACT CTCTTTGTTT CAGTTTTTAC CAGGGCTTCA TCTGCACATA TGATAGTCTT 
CATGCTTCTC AGGTGTCTGT GGGCGAGACC TTGGACAGAG TGCTATTGTT CTGCCGGTCG 
ACCGCAGGAC GTATGCTTGC TGAACTGCGG ACACTCAATA ATGACAACTT GCTGCTTAAA 
GCAGTGTGTG CAGTGAATAA TGTGGTGTAC GAGCTGTTTA ACAGGAAGCT GTATGAAGGA 
GCCTTTGGAT TGTCGATGAT TGTGTGTCAG GAGCTCTGTA AGGACTGCCC GCCCTCTCTT 
CCTATGGATC GGGTCAACCG CTGTTTCATG CTGACAGTGC AGTGTAGTCG CCGCGCAGGC 
CATCTGGAGC AGGCTCTGGA CTGGATTGTT CGCTGGATTC AGGCTTTGGG CTCACAGATT 
TTGGATCATC TCACTGAACC TGTGTCGATG TGGGTCAAAA CTAAGTGTGA TGCCGCCAGA 
GCAGGCGAGG ATGACACACG CCTGAGGACT CTGAGGGACG GGTTAGGAGA ATCCTTAGTG 
GATGAGGAGG TTATGATGTG TTTGTTAGAG GAGGAGTTGC GTGTATATAA GGAGCAGACT 
GGAGACACAG CACAGGAACG CTACAACACA CTCTGCGACC TCTTGGAAAT CTGCCATGAA 
GAAACCCCAC ACACACTAAG AAGAGCTACG TATCTGTGTG AGATGGCACA GGTTGTGTGC 
TATCAGGACT TCAGCCAGCA GACTGACTGC TCAGCAGTTG ATTTTACCCA CGAGGCTTTG 
CGGTTGCTGG AAGCAGAACC AGAGACGGCA GAGAATACTG ATAGGCTGAA GGATGAGAAA 
GCCCACGCTT CCCTGTGGCT CTATATCTGC ACTCTTGAGT CTAACCTGCA GGAGGCTGTG 
GACACAGAGA AGCGTTTGTG TGCAGTGCAG GAGGAGAATA AAAGAGATGT GAATATGGAT 
CCTGTTCCCA CAAATGATCT GGAGTACGAG GACAAACAGA AATCACAAGA GAGCCAGTTA 
GTCTACGATG GACTACGCTT CAACCTGCTG GCTCACAGCA AGCAAAGTGA ACCTCTAGAT 
AAATGTTTGT CTCTATGGCG GAGTCTCCTC AAGGGGGCTG TGCCTGCAGT TAGAGACCCT 
AAACTCACAG CTTCTTCCAT GACTTTGATG GCTGCCCTAT ACACACTTAT GGGCAAGCAC 
CTGCAAGCTC TGGAGGGATA TCAGCTTGCT GCCACCCTTT TCCATGTACT TGGTGATGGC 
CAGAGTAGTG CCAATGCCTT CTGCCACTCT ACCAGGATCT TGCTTTACCT GGGTTCACCG 
CAGCTTGCAC AGGTGGACTT AGAAAAAGCA GAAAAGAGCT TGTCATCTGT CACCATCTCT 
GAGGGCACAT CTATTATCTC CATGCTGGCC ATGTTGCAAA AAGCACAGCT ACACTATGCT 
CTAGGACAGG TGGAGAGTGG CGTGTCCTGC CTGATGGAGG TGATAAAGGA ATCTGCTCAG 
CATCATTCTA AGAGCTGGTA CCTGCTCAGG GCTCGAGCAC TGCAGACAGC CAGTGAATAC 
TTGAGTCTGG ACACACTGGC TTTGAACTCA CAGCTACGCC AGAGCATCAT GCAGCAGGGT 
CTAAAGACTC CAGACACTGC TCAATATGAA GGGTTGAAGC TGTTGTGTAG CCTGGTGATG 
ATGTTATTGG GCAACGGGTT TTACGGAGCG CCTGGACCAA ACACTGACAC TCGCTTTGTG 
GATAAAGGAG ACAGTGTTGT GTTTAAGTGG CTGCTGCTGA GTGAAGTGCT GGTGTGCTCG 
GAGAGAATGG TGATCACCAG GAGCAGCAGC GGAGCCGTTC ATGAAGCTAA AGCTCAGTGT 
CTAGAGGCTC TCAAACTGGC CACCAAACTA CAGACACTCA GCCACTGTGC TGAGCTGTTG 
GTGCTGAAGG CAGAGCTGGA GCTGATGAAG GGTGCAACTG AAGCTAGTAG TTTAGACCTA 
GAGCAAGTTA GACATTTACT TGATCTCTGC ACAGATTTTG GCCAGGGACG ACAACAGAAA 
TCTGAAGTGA AAATCAAACC ACGCAAAGGT CGTCCCGCTG CCCCTTCTTC CTCTGGTCAC 
ATCTCTGAGG AAGAGGATGA TCTGAGTGGG ATCCTCAGCT CTCGTGCCCT TCCGAAGGAG 
CCTGTGGAGA ACATGGCTAG ATTAGGAAGC CAAGGAGCTT CTCCCCCTCT AAAGCCCAAA 
TGCCAGCATG GCCTCTCCTG TCTGACCCAT GAAGAGACCT GCTCTTGTCT CTGCTGCACT 
GAGCTCAGCG TGGCTCGAGT CAGCATCCAC TGGGCACTAT TACAGGCAGA TCTCCAGACA 
GATCCCGAAC GCTCTCTCAG ACTACGCCAG TCTGCCAGGA AACGCTGCCG CTATGTTGTA 
ACTAAACTTC AGAGAAGTTT GGCAGCACTT GTACACTCCA AAAAATCACC TAATGTCTGC 
TTGTCACTGC TTCAGGCTGA ACAGGGTAAA GTGCATCTGG GTGCTGTGCT TCAACTGCTC 
AGAACTGGAG ACAAAGGGAA AGCTGCCATC TTGTGGGAGG AGATAGAAGC AGGATTAGAG 
GCAGTGACAC CTAAAGGAGC ACTGACACCA GAACTTGGGC CTATCAGAGC TACTCTACTG 
GGGGCTAAAG CCGTAGCCTG CTGCTTGGCG TTGGCAATGA AGAAACAGTG TATACCCGAA 
GAGCTGTTTT CTTCATTGTG GCACTGGAAT CCACCAAAGG TTAAACCGCA ATTAAAATCT 
AAGCATCGAT CCAAGAGTCC GGACGCAGCT CCTCTAGAGG CAGGTGTTAA ACATAACCCT 
AAAGTTAAGA GTGGACTTGA GGAGAATGAA AGCAGAAAAA CCAAAGAGAC CACTGTCATT 
TCGAAGAAAG CTAAAGACTC TGTGCCCAAA ATTACCATCA CAAAATCCTC CATGGTTTTT 
AAAACGCCCA AAGCAACGAG AACAACCCGG CCCAAATCTG TCTCCACTAG CACTGGCATC 
AGTGACCTGA GAGCATTTGA CTTCAATAAT GAGGTGCCTG ACATTTCAGT CAACTTCACA 
CCATCATTAC ACCGATCTAC AAACCAAAGT GCGACTGCAA AGTCAAAAGC GTCCTCAAAG 
GGTTCGTTCC AGGTTTACAA AGATTCTTCT CCTGCTGCAG AGAAAGCTGT AGTTGTCCCG 
GCTGCTCCTA AACGAACAAA GCGTTCTCGC TTCAAGGTTG AGTTCAGCGA TGAAAGCGAC 
AACGATGCTG CTCCTCTTGC TGTAATGGAA AAGTCAGAGA AAAAGAAAAA CACCTTTAAA 
ACATCACGCA CCTTGAAACC AACCCTTAAC ACCATCCCTA GTGACACCAA ACTTGTGAGC 
TCCACAGTAG ATCCAGAACC CTCCAGACGA ACACGGACCA CTAAGAAAAG CACAGCTCTT 
CCCTCCATTG GTGGCCTCTC TTCTGAGGAA GAACTTGGAG TATCTTCACA GACACGTACG 
CGTAGAGGAC GACCAAAGAA GAGCACCAGC TCAGCAGACA CAGAGGAGCC AGAGAGGATG 
AGGATGATTA AGGAGGAAGA ACAGAGGGTT CTTTTGAATG TCAGCATGGA GGAGCTCAGA 
GGCTCTGACA CTGAGACAAA AGACACTGGA AGTCCAGATG CTGACTGTGA GGTCTTGAGG 
AGAGATCTAG GTGCAGATTT TGGGCGAGAG TGTTTCAGTG AGCTCAGGAG AGCTGGACAG 
AATGGATCAG GATCTCACAC AGCTCTGCCA CATGCAACTA CATCTCCTGC AGACTTGTCA 
GTAGAGACGG TCCAGTCATA CCTGCGCTCC TCATGGCTCC TCCTGCACCA CTTCCCGTTT 
CCTTCTCTTT ATCCTCACAT CTGCTCATTA CTCGCTCAGT CATTCGGTCA AACGGACCCC 
ATCATCACAG CAATGCTGCA TGCACAGGCA CTGGGAGTGT CCACTCGCCA CCATATGACC 
CGTCACGTGG TTAACCAGTT CAGAAAGTTG AAGAAATCCT GTAACGATGT AGCAGAAGGT 
CTTGGAGCTC TGAGTCTGGA GGAAACATCT GGAGCAACGC AGTCAAAGAA ACTCTCTGCG 
TTGGAGCAGA TCTTTTCATT CACAGCATCA AACCCAATGC AGTTTCCACA GACACACTGT 
CAGCAGTTCA CACAGCAGCT GGAAGATCTA CCTGCAGGAG TGACCGTATG CTTGCTGTCA 
CTGACGGGGG TGCATCCTGA TGAAATTGGC AACACTATTC TTCTGACCCG ACTGGAGCGC 
GGCTCTACCC CAATCACAGT TCGAATCCCC ACTGCAGACA GAGAGCAATC TGTTGCTGGC 
CTGTTGGAAG AAATGGATGG AGTCTTGAAA GGACAAAAGG AAGTGAGCAC AGTGGCTGAG 
AAATCACAGT GGTGGGACGG AAGGAAGGCT TTAGATGCCC GTGTGGAGAA AATGCTGGAG 
GAGATGGAAG AGGCTTTAGG AGTGTGGCGA ACTCTTTTTC TGCCTTTGAC CTCTGATCCT 
AAGCTTGATG TTCAGGTAAA GAGCCTCCAA AAGGCTCTTA AATCAACAAA GATCACACGG 
GACACTCTCA AGGTGATTCT ATCAGCGTCT CCACTGCTGT CCCTGCCAGA CCTGCAGTGT 
CTTCTGGAAG GAATGAATGT GCAGGACAAG GACCTGCTGA AGCTCCTGCA GAGAGGCGTG 
GCTGAACTGA GAGGGAGGGA GGAGCCACAG GGACACACTG TTCTGATTTT AGACAAGTAC 
TTGCAGAGGT TGCCGTGGGA GAACATTGCT TGTCTGAAGT CTCAGTCGGT CACTCGAATG 
CCCTCATTAC ACGCGGTGCT TGGACACAGT CATCTGAGAG AGGTGGATAC AAGTTGTGTT 
CTATCTTGTG GTGTGAACCC TAAGCAGGTT TACTATGTGC TAAACCCAGA TGGAAACCTG 
CCCGACACTG AGAATCGCTT CAAAGAGTGG TTTACAGGTG AACGAACATG GCAGGGGGTT 
TGTGGAAGGG CTCCTGATCC GGAGAAACTA CAAGAGGCTG TGACCACTAA AGATCTCTAC 
ATATACATCG GACATGGTGC AGGAGCCCGC TTTCTGGATG CGCAGAGAGT TTTGAAGGTG 
CCTGTGAGGA CCGCAGCTCT TCTGTTCGGC TGCAGTAGTG CTGCTCTCAG TGTGCTTGGT 
CAGCAGGAGG GAACGGGCAT CATCCTCAGC TACCTGACCG CTGGATGTCC ATTGGTGCTG 
GGTAACCTGT GGGATGTGAC AGATCGTGAC TTGGATCGAT TCACATCGGC TCTGCTCCAG 
TCTTGGCTCT CTGCTGGTTC TGGCTCTTCA CTTCTTCAGC ATTTGGCGAA ATCACGCAGC 
GCAACTCATT TAAAACACAT AATCGGTGCT GCGCCCATTG CGTATGGTTT ACCGGTGCAT 
ATACACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017212151.1
CDS	138..6686
Translation	MALKSLKTEDYVQQLSCVKDTIVLHNELKKHVDGGLGPYGRTMCDRIIRACNQRLGNGAVDAGLQERIVELVELAVQGFQLLEESGMQSTPFYLEKIVFHILQKVASLGAHGPACRLGQLVYRRLKCLSAEIDDFNVLVRNCFAVLWNSLLSNKPSASTLPPRDRLHCQLQALTFRLLEQGSSSSSSPSKVPLFVEEAVVEYERSCGGFTQDDVSFLTSELRELFSGAFINPQDSASTSSLLAVWCEVAFKVSKLLCKSSFSPEAVELLRGTLKEVNGHVGLRSALGLADRAVQMQCVLSSGGECSKSFTECARALRMLPCAMSSSESHALLEACQLVVWTLEASQCKGMDVTTLLACFSFLEEYQEFLLAQQKNSFSLQVQYSLCFSFYQGFICTYDSLHASQVSVGETLDRVLLFCRSTAGRMLAELRTLNNDNLLLKAVCAVNNVVYELFNRKLYEGAFGLSMIVCQELCKDCPPSLPMDRVNRCFMLTVQCSRRAGHLEQALDWIVRWIQALGSQILDHLTEPVSMWVKTKCDAARAGEDDTRLRTLRDGLGESLVDEEVMMCLLEEELRVYKEQTGDTAQERYNTLCDLLEICHEETPHTLRRATYLCEMAQVVCYQDFSQQTDCSAVDFTHEALRLLEAEPETAENTDRLKDEKAHASLWLYICTLESNLQEAVDTEKRLCAVQEENKRDVNMDPVPTNDLEYEDKQKSQESQLVYDGLRFNLLAHSKQSEPLDKCLSLWRSLLKGAVPAVRDPKLTASSMTLMAALYTLMGKHLQALEGYQLAATLFHVLGDGQSSANAFCHSTRILLYLGSPQLAQVDLEKAEKSLSSVTISEGTSIISMLAMLQKAQLHYALGQVESGVSCLMEVIKESAQHHSKSWYLLRARALQTASEYLSLDTLALNSQLRQSIMQQGLKTPDTAQYEGLKLLCSLVMMLLGNGFYGAPGPNTDTRFVDKGDSVVFKWLLLSEVLVCSERMVITRSSSGAVHEAKAQCLEALKLATKLQTLSHCAELLVLKAELELMKGATEASSLDLEQVRHLLDLCTDFGQGRQQKSEVKIKPRKGRPAAPSSSGHISEEEDDLSGILSSRALPKEPVENMARLGSQGASPPLKPKCQHGLSCLTHEETCSCLCCTELSVARVSIHWALLQADLQTDPERSLRLRQSARKRCRYVVTKLQRSLAALVHSKKSPNVCLSLLQAEQGKVHLGAVLQLLRTGDKGKAAILWEEIEAGLEAVTPKGALTPELGPIRATLLGAKAVACCLALAMKKQCIPEELFSSLWHWNPPKVKPQLKSKHRSKSPDAAPLEAGVKHNPKVKSGLEENESRKTKETTVISKKAKDSVPKITITKSSMVFKTPKATRTTRPKSVSTSTGISDLRAFDFNNEVPDISVNFTPSLHRSTNQSATAKSKASSKGSFQVYKDSSPAAEKAVVVPAAPKRTKRSRFKVEFSDESDNDAAPLAVMEKSEKKKNTFKTSRTLKPTLNTIPSDTKLVSSTVDPEPSRRTRTTKKSTALPSIGGLSSEEELGVSSQTRTRRGRPKKSTSSADTEEPERMRMIKEEEQRVLLNVSMEELRGSDTETKDTGSPDADCEVLRRDLGADFGRECFSELRRAGQNGSGSHTALPHATTSPADLSVETVQSYLRSSWLLLHHFPFPSLYPHICSLLAQSFGQTDPIITAMLHAQALGVSTRHHMTRHVVNQFRKLKKSCNDVAEGLGALSLEETSGATQSKKLSALEQIFSFTASNPMQFPQTHCQQFTQQLEDLPAGVTVCLLSLTGVHPDEIGNTILLTRLERGSTPITVRIPTADREQSVAGLLEEMDGVLKGQKEVSTVAEKSQWWDGRKALDARVEKMLEEMEEALGVWRTLFLPLTSDPKLDVQVKSLQKALKSTKITRDTLKVILSASPLLSLPDLQCLLEGMNVQDKDLLKLLQRGVAELRGREEPQGHTVLILDKYLQRLPWENIACLKSQSVTRMPSLHAVLGHSHLREVDTSCVLSCGVNPKQVYYVLNPDGNLPDTENRFKEWFTGERTWQGVCGRAPDPEKLQEAVTTKDLYIYIGHGAGARFLDAQRVLKVPVRTAALLFGCSSAALSVLGQQEGTGIILSYLTAGCPLVLGNLWDVTDRDLDRFTSALLQSWLSAGSGSSLLQHLAKSRSATHLKHIIGAAPIAYGLPVHIH

Target ORF information:

RefSeq Version	XM_017356662.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio extra spindle pole bodies like 1, separase (espl1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017356662.1

ORF Insert Sequence:

ATGGCATTAA AGAGCTTGAA AACAGAAGAT TACGTTCAGC AGTTATCCTG TGTCAAGGAC 
ACCATAGTCC TCCACAACGA ACTGAAGAAA CATGTGGATG GTGGCTTGGG TCCTTATGGT 
CGCACAATGT GTGATCGTAT CATCAGAGCC TGCAACCAGC GGCTGGGCAA CGGCGCTGTG 
GATGCTGGCC TTCAGGAGCG GATAGTGGAG CTGGTGGAAC TGGCTGTCCA AGGGTTTCAA 
CTGCTTGAAG AGTCCGGCAT GCAGAGCACC CCCTTTTATC TGGAGAAAAT AGTCTTTCAC 
ATCCTACAGA AAGTCGCAAG CCTTGGAGCT CATGGTCCTG CTTGTCGACT GGGTCAGCTC 
GTGTATAGAA GACTGAAATG TTTGTCTGCA GAGATTGATG ACTTTAATGT CCTGGTGCGG 
AATTGCTTTG CAGTCTTGTG GAACAGTCTC CTCTCTAACA AACCGAGTGC TTCCACCCTC 
CCTCCACGAG ACAGACTCCA CTGTCAGCTG CAAGCTTTAA CATTCAGACT CCTTGAACAG 
GGCTCCAGCT CGTCCTCTTC CCCATCCAAA GTTCCTCTGT TCGTGGAGGA AGCAGTAGTG 
GAGTACGAAC GATCATGTGG AGGTTTTACT CAAGACGATG TGAGTTTTCT GACCTCTGAA 
TTGCGTGAGC TGTTCTCTGG TGCTTTCATC AATCCTCAGG ACTCTGCATC AACTTCTTCA 
CTGCTGGCTG TGTGGTGTGA GGTGGCATTT AAAGTGTCTA AACTGCTCTG CAAAAGTAGC 
TTTTCACCTG AGGCTGTTGA ACTCTTGCGA GGCACTCTGA AGGAGGTTAA TGGCCACGTT 
GGCCTGCGTT CGGCTCTGGG TCTGGCAGAC CGGGCAGTGC AGATGCAGTG TGTGTTAAGT 
TCTGGAGGTG AATGCAGTAA GAGCTTCACT GAATGTGCCC GTGCTCTCCG GATGTTGCCT 
TGTGCCATGT CCAGCTCAGA GTCTCATGCC CTGTTAGAGG CTTGTCAGCT GGTGGTTTGG 
ACGTTGGAAG CCAGTCAGTG TAAAGGAATG GATGTGACCA CTTTGCTGGC TTGTTTTTCC 
TTTTTGGAAG AATATCAGGA ATTTCTGCTT GCACAGCAAA AGAACTCGTT TTCGCTGCAG 
GTCCAATACT CTCTTTGTTT CAGTTTTTAC CAGGGCTTCA TCTGCACATA TGATAGTCTT 
CATGCTTCTC AGGTGTCTGT GGGCGAGACC TTGGACAGAG TGCTATTGTT CTGCCGGTCG 
ACCGCAGGAC GTATGCTTGC TGAACTGCGG ACACTCAATA ATGACAACTT GCTGCTTAAA 
GCAGTGTGTG CAGTGAATAA TGTGGTGTAC GAGCTGTTTA ACAGGAAGCT GTATGAAGGA 
GCCTTTGGAT TGTCGATGAT TGTGTGTCAG GAGCTCTGTA AGGACTGCCC GCCCTCTCTT 
CCTATGGATC GGGTCAACCG CTGTTTCATG CTGACAGTGC AGTGTAGTCG CCGCGCAGGC 
CATCTGGAGC AGGCTCTGGA CTGGATTGTT CGCTGGATTC AGGCTTTGGG CTCACAGATT 
TTGGATCATC TCACTGAACC TGTGTCGATG TGGGTCAAAA CTAAGTGTGA TGCCGCCAGA 
GCAGGCGAGG ATGACACACG CCTGAGGACT CTGAGGGACG GGTTAGGAGA ATCCTTAGTG 
GATGAGGAGG TTATGATGTG TTTGTTAGAG GAGGAGTTGC GTGTATATAA GGAGCAGACT 
GGAGACACAG CACAGGAACG CTACAACACA CTCTGCGACC TCTTGGAAAT CTGCCATGAA 
GAAACCCCAC ACACACTAAG AAGAGCTACG TATCTGTGTG AGATGGCACA GGTTGTGTGC 
TATCAGGACT TCAGCCAGCA GACTGACTGC TCAGCAGTTG ATTTTACCCA CGAGGCTTTG 
CGGTTGCTGG AAGCAGAACC AGAGACGGCA GAGAATACTG ATAGGCTGAA GGATGAGAAA 
GCCCACGCTT CCCTGTGGCT CTATATCTGC ACTCTTGAGT CTAACCTGCA GGAGGCTGTG 
GACACAGAGA AGCGTTTGTG TGCAGTGCAG GAGGAGAATA AAAGAGATGT GAATATGGAT 
CCTGTTCCCA CAAATGATCT GGAGTACGAG GACAAACAGA AATCACAAGA GAGCCAGTTA 
GTCTACGATG GACTACGCTT CAACCTGCTG GCTCACAGCA AGCAAAGTGA ACCTCTAGAT 
AAATGTTTGT CTCTATGGCG GAGTCTCCTC AAGGGGGCTG TGCCTGCAGT TAGAGACCCT 
AAACTCACAG CTTCTTCCAT GACTTTGATG GCTGCCCTAT ACACACTTAT GGGCAAGCAC 
CTGCAAGCTC TGGAGGGATA TCAGCTTGCT GCCACCCTTT TCCATGTACT TGGTGATGGC 
CAGAGTAGTG CCAATGCCTT CTGCCACTCT ACCAGGATCT TGCTTTACCT GGGTTCACCG 
CAGCTTGCAC AGGTGGACTT AGAAAAAGCA GAAAAGAGCT TGTCATCTGT CACCATCTCT 
GAGGGCACAT CTATTATCTC CATGCTGGCC ATGTTGCAAA AAGCACAGCT ACACTATGCT 
CTAGGACAGG TGGAGAGTGG CGTGTCCTGC CTGATGGAGG TGATAAAGGA ATCTGCTCAG 
CATCATTCTA AGAGCTGGTA CCTGCTCAGG GCTCGAGCAC TGCAGACAGC CAGTGAATAC 
TTGAGTCTGG ACACACTGGC TTTGAACTCA CAGCTACGCC AGAGCATCAT GCAGCAGGGT 
CTAAAGACTC CAGACACTGC TCAATATGAA GGGTTGAAGC TGTTGTGTAG CCTGGTGATG 
ATGTTATTGG GCAACGGGTT TTACGGAGCG CCTGGACCAA ACACTGACAC TCGCTTTGTG 
GATAAAGGAG ACAGTGTTGT GTTTAAGTGG CTGCTGCTGA GTGAAGTGCT GGTGTGCTCG 
GAGAGAATGG TGATCACCAG GAGCAGCAGC GGAGCCGTTC ATGAAGCTAA AGCTCAGTGT 
CTAGAGGCTC TCAAACTGGC CACCAAACTA CAGACACTCA GCCACTGTGC TGAGCTGTTG 
GTGCTGAAGG CAGAGCTGGA GCTGATGAAG GGTGCAACTG AAGCTAGTAG TTTAGACCTA 
GAGCAAGTTA GACATTTACT TGATCTCTGC ACAGATTTTG GCCAGGGACG ACAACAGAAA 
TCTGAAGTGA AAATCAAACC ACGCAAAGGT CGTCCCGCTG CCCCTTCTTC CTCTGGTCAC 
ATCTCTGAGG AAGAGGATGA TCTGAGTGGG ATCCTCAGCT CTCGTGCCCT TCCGAAGGAG 
CCTGTGGAGA ACATGGCTAG ATTAGGAAGC CAAGGAGCTT CTCCCCCTCT AAAGCCCAAA 
TGCCAGCATG GCCTCTCCTG TCTGACCCAT GAAGAGACCT GCTCTTGTCT CTGCTGCACT 
GAGCTCAGCG TGGCTCGAGT CAGCATCCAC TGGGCACTAT TACAGGCAGA TCTCCAGACA 
GATCCCGAAC GCTCTCTCAG ACTACGCCAG TCTGCCAGGA AACGCTGCCG CTATGTTGTA 
ACTAAACTTC AGAGAAGTTT GGCAGCACTT GTACACTCCA AAAAATCACC TAATGTCTGC 
TTGTCACTGC TTCAGGCTGA ACAGGGTAAA GTGCATCTGG GTGCTGTGCT TCAACTGCTC 
AGAACTGGAG ACAAAGGGAA AGCTGCCATC TTGTGGGAGG AGATAGAAGC AGGATTAGAG 
GCAGTGACAC CTAAAGGAGC ACTGACACCA GAACTTGGGC CTATCAGAGC TACTCTACTG 
GGGGCTAAAG CCGTAGCCTG CTGCTTGGCG TTGGCAATGA AGAAACAGTG TATACCCGAA 
GAGCTGTTTT CTTCATTGTG GCACTGGAAT CCACCAAAGG TTAAACCGCA ATTAAAATCT 
AAGCATCGAT CCAAGAGTCC GGACGCAGCT CCTCTAGAGG CAGGTGTTAA ACATAACCCT 
AAAGTTAAGA GTGGACTTGA GGAGAATGAA AGCAGAAAAA CCAAAGAGAC CACTGTCATT 
TCGAAGAAAG CTAAAGACTC TGTGCCCAAA ATTACCATCA CAAAATCCTC CATGGTTTTT 
AAAACGCCCA AAGCAACGAG AACAACCCGG CCCAAATCTG TCTCCACTAG CACTGGCATC 
AGTGACCTGA GAGCATTTGA CTTCAATAAT GAGGTGCCTG ACATTTCAGT CAACTTCACA 
CCATCATTAC ACCGATCTAC AAACCAAAGT GCGACTGCAA AGTCAAAAGC GTCCTCAAAG 
GGTTCGTTCC AGGTTTACAA AGATTCTTCT CCTGCTGCAG AGAAAGCTGT AGTTGTCCCG 
GCTGCTCCTA AACGAACAAA GCGTTCTCGC TTCAAGGTTG AGTTCAGCGA TGAAAGCGAC 
AACGATGCTG CTCCTCTTGC TGTAATGGAA AAGTCAGAGA AAAAGAAAAA CACCTTTAAA 
ACATCACGCA CCTTGAAACC AACCCTTAAC ACCATCCCTA GTGACACCAA ACTTGTGAGC 
TCCACAGTAG ATCCAGAACC CTCCAGACGA ACACGGACCA CTAAGAAAAG CACAGCTCTT 
CCCTCCATTG GTGGCCTCTC TTCTGAGGAA GAACTTGGAG TATCTTCACA GACACGTACG 
CGTAGAGGAC GACCAAAGAA GAGCACCAGC TCAGCAGACA CAGAGGAGCC AGAGAGGATG 
AGGATGATTA AGGAGGAAGA ACAGAGGGTT CTTTTGAATG TCAGCATGGA GGAGCTCAGA 
GGCTCTGACA CTGAGACAAA AGACACTGGA AGTCCAGATG CTGACTGTGA GGTCTTGAGG 
AGAGATCTAG GTGCAGATTT TGGGCGAGAG TGTTTCAGTG AGCTCAGGAG AGCTGGACAG 
AATGGATCAG GATCTCACAC AGCTCTGCCA CATGCAACTA CATCTCCTGC AGACTTGTCA 
GTAGAGACGG TCCAGTCATA CCTGCGCTCC TCATGGCTCC TCCTGCACCA CTTCCCGTTT 
CCTTCTCTTT ATCCTCACAT CTGCTCATTA CTCGCTCAGT CATTCGGTCA AACGGACCCC 
ATCATCACAG CAATGCTGCA TGCACAGGCA CTGGGAGTGT CCACTCGCCA CCATATGACC 
CGTCACGTGG TTAACCAGTT CAGAAAGTTG AAGAAATCCT GTAACGATGT AGCAGAAGGT 
CTTGGAGCTC TGAGTCTGGA GGAAACATCT GGAGCAACGC AGTCAAAGAA ACTCTCTGCG 
TTGGAGCAGA TCTTTTCATT CACAGCATCA AACCCAATGC AGTTTCCACA GACACACTGT 
CAGCAGTTCA CACAGCAGCT GGAAGATCTA CCTGCAGGAG TGACCGTATG CTTGCTGTCA 
CTGACGGGGG TGCATCCTGA TGAAATTGGC AACACTATTC TTCTGACCCG ACTGGAGCGC 
GGCTCTACCC CAATCACAGT TCGAATCCCC ACTGCAGACA GAGAGCAATC TGTTGCTGGC 
CTGTTGGAAG AAATGGATGG AGTCTTGAAA GGACAAAAGG AAGTGAGCAC AGTGGCTGAG 
AAATCACAGT GGTGGGACGG AAGGAAGGCT TTAGATGCCC GTGTGGAGAA AATGCTGGAG 
GAGATGGAAG AGGCTTTAGG AGTGTGGCGA ACTCTTTTTC TGCCTTTGAC CTCTGATCCT 
AAGCTTGATG TTCAGGTAAA GAGCCTCCAA AAGGCTCTTA AATCAACAAA GATCACACGG 
GACACTCTCA AGGTGATTCT ATCAGCGTCT CCACTGCTGT CCCTGCCAGA CCTGCAGTGT 
CTTCTGGAAG GAATGAATGT GCAGGACAAG GACCTGCTGA AGCTCCTGCA GAGAGGCGTG 
GCTGAACTGA GAGGGAGGGA GGAGCCACAG GGACACACTG TTCTGATTTT AGACAAGTAC 
TTGCAGAGGT TGCCGTGGGA GAACATTGCT TGTCTGAAGT CTCAGTCGGT CACTCGAATG 
CCCTCATTAC ACGCGGTGCT TGGACACAGT CATCTGAGAG AGGTGGATAC AAGTTGTGTT 
CTATCTTGTG GTGTGAACCC TAAGCAGGTT TACTATGTGC TAAACCCAGA TGGAAACCTG 
CCCGACACTG AGAATCGCTT CAAAGAGTGG TTTACAGGTG AACGAACATG GCAGGGGGTT 
TGTGGAAGGG CTCCTGATCC GGAGAAACTA CAAGAGGCTG TGACCACTAA AGATCTCTAC 
ATATACATCG GACATGGTGC AGGAGCCCGC TTTCTGGATG CGCAGAGAGT TTTGAAGGTG 
CCTGTGAGGA CCGCAGCTCT TCTGTTCGGC TGCAGTAGTG CTGCTCTCAG TGTGCTTGGT 
CAGCAGGAGG GAACGGGCAT CATCCTCAGC TACCTGACCG CTGGATGTCC ATTGGTGCTG 
GGTAACCTGT GGGATGTGAC AGATCGTGAC TTGGATCGAT TCACATCGGC TCTGCTCCAG 
TCTTGGCTCT CTGCTGGTTC TGGCTCTTCA CTTCTTCAGC ATTTGGCGAA ATCACGCAGC 
GCAACTCATT TAAAACACAT AATCGGTGCT GCGCCCATTG CGTATGGTTT ACCGGTGCAT 
ATACACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

espl1 ( XM_005166094.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005166151.1 Danio rerio extra spindle pole bodies like 1, separase (espl1), transcript variant X3, mRNA.

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CloneID	ODa12113
Clone ID Related Accession (Same CDS sequence)	XM_005166094.3 , XM_021477221.1 , XM_005166094.4
Accession Version	XM_005166094.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6546bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	separin isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005166094.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCATTAA AGAGCTTGAA AACAGAAGAT TACGTTCAGC AGTTATCCTG TGTCAAGGAC 
ACCATAGTCC TCCACAACGA ACTGAAGAAA CATGTGGATG GTGGCTTGGG TCCTTATGGT 
CGCACAATGT GTGATCGTAT CATCAGAGCC TGCAACCAGC GGCTGGGCAA CGGCGCTGTG 
GATGCTGGCC TTCAGGAGCG GATAGTGGAG CTGGTGGAAC TGGCTGTCCA AGGGTTTCAA 
CTGCTTGAAG AGTCCGGCAT GCAGAGCACC CCCTTTTATC TGGAGAAAAT AGTCTTTCAC 
ATCCTACAGA AAGTCGCAAG CCTTGGAGCT CATGGTCCTG CTTGTCGACT GGGTCAGCTC 
GTGTATAGAA GACTGAAATG TTTGTCTGCA GAGATTGATG ACTTTAATGT CCTGGTGCGG 
AATTGCTTTG CAGTCTTGTG GAACAGTCTC CTCTCTAACA AACCGAGTGC TTCCACCCTC 
CCTCCACGAG ACAGACTCCA CTGTCAGCTG CAAGCTTTAA CATTCAGACT CCTTGAACAG 
GGCTCCAGCT CGTCCTCTTC CCCATCCAAA GTTCCTCTGT TCGTGGAGGA AGCAGTAGTG 
GAGTACGAAC GATCATGTGG AGGTTTTACT CAAGACGATG TGAGTTTTCT GACCTCTGAA 
TTGCGTGAGC TGTTCTCTGG TGCTTTCATC AATCCTCAGG ACTCTGCATC AACTTCTTCA 
CTGCTGGCTG TGTGGTGTGA GGTGGCATTT AAAGTGTCTA AACTGCTCTG CAAAAGTAGC 
TTTTCACCTG AGGCTGTTGA ACTCTTGCGA GGCACTCTGA AGGAGGTTAA TGGCCACGTT 
GGCCTGCGTT CGGCTCTGGG TCTGGCAGAC CGGGCAGTGC AGATGCAGTG TGTGTTAAGT 
TCTGGAGGTG AATGCAGTAA GAGCTTCACT GAATGTGCCC GTGCTCTCCG GATGTTGCCT 
TGTGCCATGT CCAGCTCAGA GTCTCATGCC CTGTTAGAGG CTTGTCAGCT GGTGGTTTGG 
ACGTTGGAAG CCAGTCAGTG TAAAGGAATG GATGTGACCA CTTTGCTGGC TTGTTTTTCC 
TTTTTGGAAG AATATCAGGA ATTTCTGCTT GCACAGCAAA AGAACTCGTT TTCGCTGCAG 
GTCCAATACT CTCTTTGTTT CAGTTTTTAC CAGGGCTTCA TCTGCACATA TGATAGTCTT 
CATGCTTCTC AGGTGTCTGT GGGCGAGACC TTGGACAGAG TGCTATTGTT CTGCCGGTCG 
ACCGCAGGAC GTATGCTTGC TGAACTGCGG ACACTCAATA ATGACAACTT GCTGCTTAAA 
GCAGTGTGTG CAGTGAATAA TGTGGTGTAC GAGCTGTTTA ACAGGAAGCT GTATGAAGGA 
GCCTTTGGAT TGTCGATGAT TGTGTGTCAG GAGCTCTGTA AGGACTGCCC GCCCTCTCTT 
CCTATGGATC GGGTCAACCG CTGTTTCATG CTGACAGTGC AGTGTAGTCG CCGCGCAGGC 
CATCTGGAGC AGGCTCTGGA CTGGATTGTT CGCTGGATTC AGGCTTTGGG CTCACAGATT 
TTGGATCATC TCACTGAACC TGTGTCGATG TGGGTCAAAA CTAAGTGTGA TGCCGCCAGA 
GCAGGCGAGG ATGACACACG CCTGAGGACT CTGAGGGACG GGTTAGGAGA ATCCTTAGTG 
GATGAGGAGG TTATGATGTG TTTGTTAGAG GAGGAGTTGC GTGTATATAA GGAGCAGACT 
GGAGACACAG CACAGGAACG CTACAACACA CTCTGCGACC TCTTGGAAAT CTGCCATGAA 
GAAACCCCAC ACACACTAAG AAGAGCTACG TATCTGTGTG AGATGGCACA GGTTGTGTGC 
TATCAGGACT TCAGCCAGCA GACTGACTGC TCAGCAGTTG ATTTTACCCA CGAGGCTTTG 
CGGTTGCTGG AAGCAGAACC AGAGACGGCA GAGAATACTG ATAGGCTGAA GGATGAGAAA 
GCCCACGCTT CCCTGTGGCT CTATATCTGC ACTCTTGAGT CTAACCTGCA GGAGGCTGTG 
GACACAGAGA AGCGTTTGTG TGCAGTGCAG GAGGAGAATA AAAGAGATGT GAATATGGAT 
CCTGTTCCCA CAAATGATCT GGAGTACGAG GACAAACAGA AATCACAAGA GAGCCAGTTA 
GTCTACGATG GACTACGCTT CAACCTGCTG GCTCACAGCA AGCAAAGTGA ACCTCTAGAT 
AAATGTTTGT CTCTATGGCG GAGTCTCCTC AAGGGGGCTG TGCCTGCAGT TAGAGACCCT 
AAACTCACAG CTTCTTCCAT GACTTTGATG GCTGCCCTAT ACACACTTAT GGGCAAGCAC 
CTGCAAGCTC TGGAGGGATA TCAGCTTGCT GCCACCCTTT TCCATGTACT TGGTGATGGC 
CAGAGTAGTG CCAATGCCTT CTGCCACTCT ACCAGGATCT TGCTTTACCT GGGTTCACCG 
CAGCTTGCAC AGGTGGACTT AGAAAAAGCA GAAAAGAGCT TGTCATCTGT CACCATCTCT 
GAGGGCACAT CTATTATCTC CATGCTGGCC ATGTTGCAAA AAGCACAGCT ACACTATGCT 
CTAGGACAGG TGGAGAGTGG CGTGTCCTGC CTGATGGAGG TGATAAAGGA ATCTGCTCAG 
CATCATTCTA AGAGCTGGTA CCTGCTCAGG GCTCGAGCAC TGCAGACAGC CAGTGAATAC 
TTGAGTCTGG ACACACTGGC TTTGAACTCA CAGCTACGCC AGAGCATCAT GCAGCAGGGT 
CTAAAGACTC CAGACACTGC TCAATATGAA GGGTTGAAGC TGTTGTGTAG CCTGGTGATG 
ATGTTATTGG GCAACGGGTT TTACGGAGCG CCTGGACCAA ACACTGACAC TCGCTTTGTG 
GATAAAGGAG ACAGTGTTGT GTTTAAGTGG CTGCTGCTGA GTGAAGTGCT GGTGTGCTCG 
GAGAGAATGG TGATCACCAG GAGCAGCAGC GGAGCCGTTC ATGAAGCTAA AGCTCAGTGT 
CTAGAGGCTC TCAAACTGGC CACCAAACTA CAGACACTCA GCCACTGTGC TGAGCTGTTG 
GTGCTGAAGG CAGAGCTGGA GCTGATGAAG GGTGCAACTG AAGCTAGTAG TTTAGACCTA 
GAGCAAGTTA GACATTTACT TGATCTCTGC ACAGATTTTG GCCAGGGACG ACAACAGAAA 
TCTGAAGTGA AAATCAAACC ACGCAAAGGT CGTCCCGCTG CCCCTTCTTC CTCTGGTCAC 
ATCTCTGAGG AAGAGGATGA TCTGAGTGGG ATCCTCAGCT CTCGTGCCCT TCCGAAGGAG 
CCTGTGGAGA ACATGGCTAG ATTAGGAAGC CAAGGAGCTT CTCCCCCTCT AAAGCCCAAA 
TGCCAGCATG GCCTCTCCTG TCTGACCCAT GAAGAGACCT GCTCTTGTCT CTGCTGCACT 
GAGCTCAGCG TGGCTCGAGT CAGCATCCAC TGGGCACTAT TACAGGCAGA TCTCCAGACA 
GATCCCGAAC GCTCTCTCAG ACTACGCCAG TCTGCCAGGA AACGCTGCCG CTATGTTGTA 
ACTAAACTTC AGAGAAGTTT GGCAGCACTT GTACACTCCA AAAAATCACC TAATGTCTGC 
TTGTCACTGC TTCAGGCTGA ACAGGGTAAA GTGCATCTGG GTGCTGTGCT TCAACTGCTC 
AGAACTGGAG ACAAAGGGAA AGCTGCCATC TTGTGGGAGG AGATAGAAGC AGGATTAGAG 
GCAGTGACAC CTAAAGGAGC ACTGACACCA GAACTTGGGC CTATCAGAGC TACTCTACTG 
GGGGCTAAAG CCGTAGCCTG CTGCTTGGCG TTGGCAATGA AGAAACAGTG TATACCCGAA 
GAGCTGTTTT CTTCATTGTG GCACTGGAAT CCACCAAAGG TTAAACCGCA ATTAAAATCT 
AAGCATCGAT CCAAGAGTCC GGACGCAGCT CCTCTAGAGG CAGGTGTTAA ACATAACCCT 
AAAGTTAAGA GTGGACTTGA GGAGAATGAA AGCAGAAAAA CCAAAGAGAC CACTGTCATT 
TCGAAGAAAG CTAAAGACTC TGTGCCCAAA ATTACCATCA CAAAATCCTC CATGGTTTTT 
AAAACGCCCA AAGCAACGAG AACAACCCGG CCCAAATCTG TCTCCACTAG CACTGGCATC 
AGTGACCTGA GAGCATTTGA CTTCAATAAT GAGGTGCCTG ACATTTCAGT CAACTTCACA 
CCATCATTAC ACCGATCTAC AAACCAAAGT GCGACTGCAA AGTCAAAAGC GTCCTCAAAG 
GGTTCGTTCC AGGTTTACAA AGATTCTTCT CCTGCTGCAG AGAAAGCTGT AGTTGTCCCG 
GCTGCTCCTA AACGAACAAA GCGTTCTCGC TTCAAGGTTG AGTTCAGCGA TGAAAGCGAC 
AACGATGCTG CTCCTCTTGC TGTAATGGAA AAGTCAGAGA AAAAGAAAAA CACCTTTAAA 
ACATCACGCA CCTTGAAACC AACCCTTAAC ACCATCCCTA GTGACACCAA ACTTGTGAGC 
TCCACAGTAG ATCCAGAACC CTCCAGACGA ACACGGACCA CTAAGAAAAG CACAGCTCTT 
CCCTCCATTG GTGGCCTCTC TTCTGAGGAA GAACTTGGAG TATCTTCACA GACACGTACG 
CGTAGAGGAC GACCAAAGAA GAGCACCAGC TCAGCAGACA CAGAGGAGCC AGAGAGGATG 
AGGATGATTA AGGAGGAAGA ACAGAGGGTT CTTTTGAATG TCAGCATGGA GGAGCTCAGA 
GGCTCTGACA CTGAGACAAA AGACACTGGA AGTCCAGATG CTGACTGTGA GGTCTTGAGG 
AGAGATCTAG GTGCAGATTT TGGGCGAGAG TGTTTCAGTG AGCTCAGGAG AGCTGGACAG 
AATGGATCAG GATCTCACAC AGCTCTGCCA CATGCAACTA CATCTCCTGA CTTGTCAGTA 
GAGACGGTCC AGTCATACCT GCGCTCCTCA TGGCTCCTCC TGCACCACTT CCCGTTTCCT 
TCTCTTTATC CTCACATCTG CTCATTACTC GCTCAGTCAT TCGGTCAAAC GGACCCCATC 
ATCACAGCAA TGCTGCATGC ACAGGCACTG GGAGTGTCCA CTCGCCACCA TATGACCCGT 
CACGTGGTTA ACCAGTTCAG AAAGTTGAAG AAATCCTGTA ACGATGTAGC AGAAGGTCTT 
GGAGCTCTGA GTCTGGAGGA AACATCTGGA GCAACGCAGT CAAAGAAACT CTCTGCGTTG 
GAGCAGATCT TTTCATTCAC AGCATCAAAC CCAATGCAGT TTCCACAGAC ACACTGTCAG 
CAGTTCACAC AGCAGCTGGA AGATCTACCT GCAGGAGTGA CCGTATGCTT GCTGTCACTG 
ACGGGGGTGC ATCCTGATGA AATTGGCAAC ACTATTCTTC TGACCCGACT GGAGCGCGGC 
TCTACCCCAA TCACAGTTCG AATCCCCACT GCAGACAGAG AGCAATCTGT TGCTGGCCTG 
TTGGAAGAAA TGGATGGAGT CTTGAAAGGA CAAAAGGAAG TGAGCACAGT GGCTGAGAAA 
TCACAGTGGT GGGACGGAAG GAAGGCTTTA GATGCCCGTG TGGAGAAAAT GCTGGAGGAG 
ATGGAAGAGG CTTTAGGAGT GTGGCGAACT CTTTTTCTGC CTTTGACCTC TGATCCTAAG 
CTTGATGTTC AGGTAAAGAG CCTCCAAAAG GCTCTTAAAT CAACAAAGAT CACACGGGAC 
ACTCTCAAGG TGATTCTATC AGCGTCTCCA CTGCTGTCCC TGCCAGACCT GCAGTGTCTT 
CTGGAAGGAA TGAATGTGCA GGACAAGGAC CTGCTGAAGC TCCTGCAGAG AGGCGTGGCT 
GAACTGAGAG GGAGGGAGGA GCCACAGGGA CACACTGTTC TGATTTTAGA CAAGTACTTG 
CAGAGGTTGC CGTGGGAGAA CATTGCTTGT CTGAAGTCTC AGTCGGTCAC TCGAATGCCC 
TCATTACACG CGGTGCTTGG ACACAGTCAT CTGAGAGAGG TGGATACAAG TTGTGTTCTA 
TCTTGTGGTG TGAACCCTAA GCAGGTTTAC TATGTGCTAA ACCCAGATGG AAACCTGCCC 
GACACTGAGA ATCGCTTCAA AGAGTGGTTT ACAGGTGAAC GAACATGGCA GGGGGTTTGT 
GGAAGGGCTC CTGATCCGGA GAAACTACAA GAGGCTGTGA CCACTAAAGA TCTCTACATA 
TACATCGGAC ATGGTGCAGG AGCCCGCTTT CTGGATGCGC AGAGAGTTTT GAAGGTGCCT 
GTGAGGACCG CAGCTCTTCT GTTCGGCTGC AGTAGTGCTG CTCTCAGTGT GCTTGGTCAG 
CAGGAGGGAA CGGGCATCAT CCTCAGCTAC CTGACCGCTG GATGTCCATT GGTGCTGGGT 
AACCTGTGGG ATGTGACAGA TCGTGACTTG GATCGATTCA CATCGGCTCT GCTCCAGTCT 
TGGCTCTCTG CTGGTTCTGG CTCTTCACTT CTTCAGCATT TGGCGAAATC ACGCAGCGCA 
ACTCATTTAA AACACATAAT CGGTGCTGCG CCCATTGCGT ATGGTTTACC GGTGCATATA 
CACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005166151.1
CDS	187..6732
Translation	MALKSLKTEDYVQQLSCVKDTIVLHNELKKHVDGGLGPYGRTMCDRIIRACNQRLGNGAVDAGLQERIVELVELAVQGFQLLEESGMQSTPFYLEKIVFHILQKVASLGAHGPACRLGQLVYRRLKCLSAEIDDFNVLVRNCFAVLWNSLLSNKPSASTLPPRDRLHCQLQALTFRLLEQGSSSSSSPSKVPLFVEEAVVEYERSCGGFTQDDVSFLTSELRELFSGAFINPQDSASTSSLLAVWCEVAFKVSKLLCKSSFSPEAVELLRGTLKEVNGHVGLRSALGLADRAVQMQCVLSSGGECSKSFTECARALRMLPCAMSSSESHALLEACQLVVWTLEASQCKGMDVTTLLACFSFLEEYQEFLLAQQKNSFSLQVQYSLCFSFYQGFICTYDSLHASQVSVGETLDRVLLFCRSTAGRMLAELRTLNNDNLLLKAVCAVNNVVYELFNRKLYEGAFGLSMIVCQELCKDCPPSLPMDRVNRCFMLTVQCSRRAGHLEQALDWIVRWIQALGSQILDHLTEPVSMWVKTKCDAARAGEDDTRLRTLRDGLGESLVDEEVMMCLLEEELRVYKEQTGDTAQERYNTLCDLLEICHEETPHTLRRATYLCEMAQVVCYQDFSQQTDCSAVDFTHEALRLLEAEPETAENTDRLKDEKAHASLWLYICTLESNLQEAVDTEKRLCAVQEENKRDVNMDPVPTNDLEYEDKQKSQESQLVYDGLRFNLLAHSKQSEPLDKCLSLWRSLLKGAVPAVRDPKLTASSMTLMAALYTLMGKHLQALEGYQLAATLFHVLGDGQSSANAFCHSTRILLYLGSPQLAQVDLEKAEKSLSSVTISEGTSIISMLAMLQKAQLHYALGQVESGVSCLMEVIKESAQHHSKSWYLLRARALQTASEYLSLDTLALNSQLRQSIMQQGLKTPDTAQYEGLKLLCSLVMMLLGNGFYGAPGPNTDTRFVDKGDSVVFKWLLLSEVLVCSERMVITRSSSGAVHEAKAQCLEALKLATKLQTLSHCAELLVLKAELELMKGATEASSLDLEQVRHLLDLCTDFGQGRQQKSEVKIKPRKGRPAAPSSSGHISEEEDDLSGILSSRALPKEPVENMARLGSQGASPPLKPKCQHGLSCLTHEETCSCLCCTELSVARVSIHWALLQADLQTDPERSLRLRQSARKRCRYVVTKLQRSLAALVHSKKSPNVCLSLLQAEQGKVHLGAVLQLLRTGDKGKAAILWEEIEAGLEAVTPKGALTPELGPIRATLLGAKAVACCLALAMKKQCIPEELFSSLWHWNPPKVKPQLKSKHRSKSPDAAPLEAGVKHNPKVKSGLEENESRKTKETTVISKKAKDSVPKITITKSSMVFKTPKATRTTRPKSVSTSTGISDLRAFDFNNEVPDISVNFTPSLHRSTNQSATAKSKASSKGSFQVYKDSSPAAEKAVVVPAAPKRTKRSRFKVEFSDESDNDAAPLAVMEKSEKKKNTFKTSRTLKPTLNTIPSDTKLVSSTVDPEPSRRTRTTKKSTALPSIGGLSSEEELGVSSQTRTRRGRPKKSTSSADTEEPERMRMIKEEEQRVLLNVSMEELRGSDTETKDTGSPDADCEVLRRDLGADFGRECFSELRRAGQNGSGSHTALPHATTSPDLSVETVQSYLRSSWLLLHHFPFPSLYPHICSLLAQSFGQTDPIITAMLHAQALGVSTRHHMTRHVVNQFRKLKKSCNDVAEGLGALSLEETSGATQSKKLSALEQIFSFTASNPMQFPQTHCQQFTQQLEDLPAGVTVCLLSLTGVHPDEIGNTILLTRLERGSTPITVRIPTADREQSVAGLLEEMDGVLKGQKEVSTVAEKSQWWDGRKALDARVEKMLEEMEEALGVWRTLFLPLTSDPKLDVQVKSLQKALKSTKITRDTLKVILSASPLLSLPDLQCLLEGMNVQDKDLLKLLQRGVAELRGREEPQGHTVLILDKYLQRLPWENIACLKSQSVTRMPSLHAVLGHSHLREVDTSCVLSCGVNPKQVYYVLNPDGNLPDTENRFKEWFTGERTWQGVCGRAPDPEKLQEAVTTKDLYIYIGHGAGARFLDAQRVLKVPVRTAALLFGCSSAALSVLGQQEGTGIILSYLTAGCPLVLGNLWDVTDRDLDRFTSALLQSWLSAGSGSSLLQHLAKSRSATHLKHIIGAAPIAYGLPVHIH

Target ORF information:

RefSeq Version	XM_005166094.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio extra spindle pole bodies like 1, separase (espl1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005166094.3

ORF Insert Sequence:

ATGGCATTAA AGAGCTTGAA AACAGAAGAT TACGTTCAGC AGTTATCCTG TGTCAAGGAC 
ACCATAGTCC TCCACAACGA ACTGAAGAAA CATGTGGATG GTGGCTTGGG TCCTTATGGT 
CGCACAATGT GTGATCGTAT CATCAGAGCC TGCAACCAGC GGCTGGGCAA CGGCGCTGTG 
GATGCTGGCC TTCAGGAGCG GATAGTGGAG CTGGTGGAAC TGGCTGTCCA AGGGTTTCAA 
CTGCTTGAAG AGTCCGGCAT GCAGAGCACC CCCTTTTATC TGGAGAAAAT AGTCTTTCAC 
ATCCTACAGA AAGTCGCAAG CCTTGGAGCT CATGGTCCTG CTTGTCGACT GGGTCAGCTC 
GTGTATAGAA GACTGAAATG TTTGTCTGCA GAGATTGATG ACTTTAATGT CCTGGTGCGG 
AATTGCTTTG CAGTCTTGTG GAACAGTCTC CTCTCTAACA AACCGAGTGC TTCCACCCTC 
CCTCCACGAG ACAGACTCCA CTGTCAGCTG CAAGCTTTAA CATTCAGACT CCTTGAACAG 
GGCTCCAGCT CGTCCTCTTC CCCATCCAAA GTTCCTCTGT TCGTGGAGGA AGCAGTAGTG 
GAGTACGAAC GATCATGTGG AGGTTTTACT CAAGACGATG TGAGTTTTCT GACCTCTGAA 
TTGCGTGAGC TGTTCTCTGG TGCTTTCATC AATCCTCAGG ACTCTGCATC AACTTCTTCA 
CTGCTGGCTG TGTGGTGTGA GGTGGCATTT AAAGTGTCTA AACTGCTCTG CAAAAGTAGC 
TTTTCACCTG AGGCTGTTGA ACTCTTGCGA GGCACTCTGA AGGAGGTTAA TGGCCACGTT 
GGCCTGCGTT CGGCTCTGGG TCTGGCAGAC CGGGCAGTGC AGATGCAGTG TGTGTTAAGT 
TCTGGAGGTG AATGCAGTAA GAGCTTCACT GAATGTGCCC GTGCTCTCCG GATGTTGCCT 
TGTGCCATGT CCAGCTCAGA GTCTCATGCC CTGTTAGAGG CTTGTCAGCT GGTGGTTTGG 
ACGTTGGAAG CCAGTCAGTG TAAAGGAATG GATGTGACCA CTTTGCTGGC TTGTTTTTCC 
TTTTTGGAAG AATATCAGGA ATTTCTGCTT GCACAGCAAA AGAACTCGTT TTCGCTGCAG 
GTCCAATACT CTCTTTGTTT CAGTTTTTAC CAGGGCTTCA TCTGCACATA TGATAGTCTT 
CATGCTTCTC AGGTGTCTGT GGGCGAGACC TTGGACAGAG TGCTATTGTT CTGCCGGTCG 
ACCGCAGGAC GTATGCTTGC TGAACTGCGG ACACTCAATA ATGACAACTT GCTGCTTAAA 
GCAGTGTGTG CAGTGAATAA TGTGGTGTAC GAGCTGTTTA ACAGGAAGCT GTATGAAGGA 
GCCTTTGGAT TGTCGATGAT TGTGTGTCAG GAGCTCTGTA AGGACTGCCC GCCCTCTCTT 
CCTATGGATC GGGTCAACCG CTGTTTCATG CTGACAGTGC AGTGTAGTCG CCGCGCAGGC 
CATCTGGAGC AGGCTCTGGA CTGGATTGTT CGCTGGATTC AGGCTTTGGG CTCACAGATT 
TTGGATCATC TCACTGAACC TGTGTCGATG TGGGTCAAAA CTAAGTGTGA TGCCGCCAGA 
GCAGGCGAGG ATGACACACG CCTGAGGACT CTGAGGGACG GGTTAGGAGA ATCCTTAGTG 
GATGAGGAGG TTATGATGTG TTTGTTAGAG GAGGAGTTGC GTGTATATAA GGAGCAGACT 
GGAGACACAG CACAGGAACG CTACAACACA CTCTGCGACC TCTTGGAAAT CTGCCATGAA 
GAAACCCCAC ACACACTAAG AAGAGCTACG TATCTGTGTG AGATGGCACA GGTTGTGTGC 
TATCAGGACT TCAGCCAGCA GACTGACTGC TCAGCAGTTG ATTTTACCCA CGAGGCTTTG 
CGGTTGCTGG AAGCAGAACC AGAGACGGCA GAGAATACTG ATAGGCTGAA GGATGAGAAA 
GCCCACGCTT CCCTGTGGCT CTATATCTGC ACTCTTGAGT CTAACCTGCA GGAGGCTGTG 
GACACAGAGA AGCGTTTGTG TGCAGTGCAG GAGGAGAATA AAAGAGATGT GAATATGGAT 
CCTGTTCCCA CAAATGATCT GGAGTACGAG GACAAACAGA AATCACAAGA GAGCCAGTTA 
GTCTACGATG GACTACGCTT CAACCTGCTG GCTCACAGCA AGCAAAGTGA ACCTCTAGAT 
AAATGTTTGT CTCTATGGCG GAGTCTCCTC AAGGGGGCTG TGCCTGCAGT TAGAGACCCT 
AAACTCACAG CTTCTTCCAT GACTTTGATG GCTGCCCTAT ACACACTTAT GGGCAAGCAC 
CTGCAAGCTC TGGAGGGATA TCAGCTTGCT GCCACCCTTT TCCATGTACT TGGTGATGGC 
CAGAGTAGTG CCAATGCCTT CTGCCACTCT ACCAGGATCT TGCTTTACCT GGGTTCACCG 
CAGCTTGCAC AGGTGGACTT AGAAAAAGCA GAAAAGAGCT TGTCATCTGT CACCATCTCT 
GAGGGCACAT CTATTATCTC CATGCTGGCC ATGTTGCAAA AAGCACAGCT ACACTATGCT 
CTAGGACAGG TGGAGAGTGG CGTGTCCTGC CTGATGGAGG TGATAAAGGA ATCTGCTCAG 
CATCATTCTA AGAGCTGGTA CCTGCTCAGG GCTCGAGCAC TGCAGACAGC CAGTGAATAC 
TTGAGTCTGG ACACACTGGC TTTGAACTCA CAGCTACGCC AGAGCATCAT GCAGCAGGGT 
CTAAAGACTC CAGACACTGC TCAATATGAA GGGTTGAAGC TGTTGTGTAG CCTGGTGATG 
ATGTTATTGG GCAACGGGTT TTACGGAGCG CCTGGACCAA ACACTGACAC TCGCTTTGTG 
GATAAAGGAG ACAGTGTTGT GTTTAAGTGG CTGCTGCTGA GTGAAGTGCT GGTGTGCTCG 
GAGAGAATGG TGATCACCAG GAGCAGCAGC GGAGCCGTTC ATGAAGCTAA AGCTCAGTGT 
CTAGAGGCTC TCAAACTGGC CACCAAACTA CAGACACTCA GCCACTGTGC TGAGCTGTTG 
GTGCTGAAGG CAGAGCTGGA GCTGATGAAG GGTGCAACTG AAGCTAGTAG TTTAGACCTA 
GAGCAAGTTA GACATTTACT TGATCTCTGC ACAGATTTTG GCCAGGGACG ACAACAGAAA 
TCTGAAGTGA AAATCAAACC ACGCAAAGGT CGTCCCGCTG CCCCTTCTTC CTCTGGTCAC 
ATCTCTGAGG AAGAGGATGA TCTGAGTGGG ATCCTCAGCT CTCGTGCCCT TCCGAAGGAG 
CCTGTGGAGA ACATGGCTAG ATTAGGAAGC CAAGGAGCTT CTCCCCCTCT AAAGCCCAAA 
TGCCAGCATG GCCTCTCCTG TCTGACCCAT GAAGAGACCT GCTCTTGTCT CTGCTGCACT 
GAGCTCAGCG TGGCTCGAGT CAGCATCCAC TGGGCACTAT TACAGGCAGA TCTCCAGACA 
GATCCCGAAC GCTCTCTCAG ACTACGCCAG TCTGCCAGGA AACGCTGCCG CTATGTTGTA 
ACTAAACTTC AGAGAAGTTT GGCAGCACTT GTACACTCCA AAAAATCACC TAATGTCTGC 
TTGTCACTGC TTCAGGCTGA ACAGGGTAAA GTGCATCTGG GTGCTGTGCT TCAACTGCTC 
AGAACTGGAG ACAAAGGGAA AGCTGCCATC TTGTGGGAGG AGATAGAAGC AGGATTAGAG 
GCAGTGACAC CTAAAGGAGC ACTGACACCA GAACTTGGGC CTATCAGAGC TACTCTACTG 
GGGGCTAAAG CCGTAGCCTG CTGCTTGGCG TTGGCAATGA AGAAACAGTG TATACCCGAA 
GAGCTGTTTT CTTCATTGTG GCACTGGAAT CCACCAAAGG TTAAACCGCA ATTAAAATCT 
AAGCATCGAT CCAAGAGTCC GGACGCAGCT CCTCTAGAGG CAGGTGTTAA ACATAACCCT 
AAAGTTAAGA GTGGACTTGA GGAGAATGAA AGCAGAAAAA CCAAAGAGAC CACTGTCATT 
TCGAAGAAAG CTAAAGACTC TGTGCCCAAA ATTACCATCA CAAAATCCTC CATGGTTTTT 
AAAACGCCCA AAGCAACGAG AACAACCCGG CCCAAATCTG TCTCCACTAG CACTGGCATC 
AGTGACCTGA GAGCATTTGA CTTCAATAAT GAGGTGCCTG ACATTTCAGT CAACTTCACA 
CCATCATTAC ACCGATCTAC AAACCAAAGT GCGACTGCAA AGTCAAAAGC GTCCTCAAAG 
GGTTCGTTCC AGGTTTACAA AGATTCTTCT CCTGCTGCAG AGAAAGCTGT AGTTGTCCCG 
GCTGCTCCTA AACGAACAAA GCGTTCTCGC TTCAAGGTTG AGTTCAGCGA TGAAAGCGAC 
AACGATGCTG CTCCTCTTGC TGTAATGGAA AAGTCAGAGA AAAAGAAAAA CACCTTTAAA 
ACATCACGCA CCTTGAAACC AACCCTTAAC ACCATCCCTA GTGACACCAA ACTTGTGAGC 
TCCACAGTAG ATCCAGAACC CTCCAGACGA ACACGGACCA CTAAGAAAAG CACAGCTCTT 
CCCTCCATTG GTGGCCTCTC TTCTGAGGAA GAACTTGGAG TATCTTCACA GACACGTACG 
CGTAGAGGAC GACCAAAGAA GAGCACCAGC TCAGCAGACA CAGAGGAGCC AGAGAGGATG 
AGGATGATTA AGGAGGAAGA ACAGAGGGTT CTTTTGAATG TCAGCATGGA GGAGCTCAGA 
GGCTCTGACA CTGAGACAAA AGACACTGGA AGTCCAGATG CTGACTGTGA GGTCTTGAGG 
AGAGATCTAG GTGCAGATTT TGGGCGAGAG TGTTTCAGTG AGCTCAGGAG AGCTGGACAG 
AATGGATCAG GATCTCACAC AGCTCTGCCA CATGCAACTA CATCTCCTGA CTTGTCAGTA 
GAGACGGTCC AGTCATACCT GCGCTCCTCA TGGCTCCTCC TGCACCACTT CCCGTTTCCT 
TCTCTTTATC CTCACATCTG CTCATTACTC GCTCAGTCAT TCGGTCAAAC GGACCCCATC 
ATCACAGCAA TGCTGCATGC ACAGGCACTG GGAGTGTCCA CTCGCCACCA TATGACCCGT 
CACGTGGTTA ACCAGTTCAG AAAGTTGAAG AAATCCTGTA ACGATGTAGC AGAAGGTCTT 
GGAGCTCTGA GTCTGGAGGA AACATCTGGA GCAACGCAGT CAAAGAAACT CTCTGCGTTG 
GAGCAGATCT TTTCATTCAC AGCATCAAAC CCAATGCAGT TTCCACAGAC ACACTGTCAG 
CAGTTCACAC AGCAGCTGGA AGATCTACCT GCAGGAGTGA CCGTATGCTT GCTGTCACTG 
ACGGGGGTGC ATCCTGATGA AATTGGCAAC ACTATTCTTC TGACCCGACT GGAGCGCGGC 
TCTACCCCAA TCACAGTTCG AATCCCCACT GCAGACAGAG AGCAATCTGT TGCTGGCCTG 
TTGGAAGAAA TGGATGGAGT CTTGAAAGGA CAAAAGGAAG TGAGCACAGT GGCTGAGAAA 
TCACAGTGGT GGGACGGAAG GAAGGCTTTA GATGCCCGTG TGGAGAAAAT GCTGGAGGAG 
ATGGAAGAGG CTTTAGGAGT GTGGCGAACT CTTTTTCTGC CTTTGACCTC TGATCCTAAG 
CTTGATGTTC AGGTAAAGAG CCTCCAAAAG GCTCTTAAAT CAACAAAGAT CACACGGGAC 
ACTCTCAAGG TGATTCTATC AGCGTCTCCA CTGCTGTCCC TGCCAGACCT GCAGTGTCTT 
CTGGAAGGAA TGAATGTGCA GGACAAGGAC CTGCTGAAGC TCCTGCAGAG AGGCGTGGCT 
GAACTGAGAG GGAGGGAGGA GCCACAGGGA CACACTGTTC TGATTTTAGA CAAGTACTTG 
CAGAGGTTGC CGTGGGAGAA CATTGCTTGT CTGAAGTCTC AGTCGGTCAC TCGAATGCCC 
TCATTACACG CGGTGCTTGG ACACAGTCAT CTGAGAGAGG TGGATACAAG TTGTGTTCTA 
TCTTGTGGTG TGAACCCTAA GCAGGTTTAC TATGTGCTAA ACCCAGATGG AAACCTGCCC 
GACACTGAGA ATCGCTTCAA AGAGTGGTTT ACAGGTGAAC GAACATGGCA GGGGGTTTGT 
GGAAGGGCTC CTGATCCGGA GAAACTACAA GAGGCTGTGA CCACTAAAGA TCTCTACATA 
TACATCGGAC ATGGTGCAGG AGCCCGCTTT CTGGATGCGC AGAGAGTTTT GAAGGTGCCT 
GTGAGGACCG CAGCTCTTCT GTTCGGCTGC AGTAGTGCTG CTCTCAGTGT GCTTGGTCAG 
CAGGAGGGAA CGGGCATCAT CCTCAGCTAC CTGACCGCTG GATGTCCATT GGTGCTGGGT 
AACCTGTGGG ATGTGACAGA TCGTGACTTG GATCGATTCA CATCGGCTCT GCTCCAGTCT 
TGGCTCTCTG CTGGTTCTGG CTCTTCACTT CTTCAGCATT TGGCGAAATC ACGCAGCGCA 
ACTCATTTAA AACACATAAT CGGTGCTGCG CCCATTGCGT ATGGTTTACC GGTGCATATA 
CACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

espl1 ( XM_021477221.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021332896.1 Danio rerio extra spindle pole bodies like 1, separase (espl1), transcript variant X2, mRNA.

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CloneID	ODa12113
Clone ID Related Accession (Same CDS sequence)	XM_005166094.3 , XM_021477221.1 , XM_005166094.4
Accession Version	XM_021477221.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6546bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	separin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCATTAA AGAGCTTGAA AACAGAAGAT TACGTTCAGC AGTTATCCTG TGTCAAGGAC 
ACCATAGTCC TCCACAACGA ACTGAAGAAA CATGTGGATG GTGGCTTGGG TCCTTATGGT 
CGCACAATGT GTGATCGTAT CATCAGAGCC TGCAACCAGC GGCTGGGCAA CGGCGCTGTG 
GATGCTGGCC TTCAGGAGCG GATAGTGGAG CTGGTGGAAC TGGCTGTCCA AGGGTTTCAA 
CTGCTTGAAG AGTCCGGCAT GCAGAGCACC CCCTTTTATC TGGAGAAAAT AGTCTTTCAC 
ATCCTACAGA AAGTCGCAAG CCTTGGAGCT CATGGTCCTG CTTGTCGACT GGGTCAGCTC 
GTGTATAGAA GACTGAAATG TTTGTCTGCA GAGATTGATG ACTTTAATGT CCTGGTGCGG 
AATTGCTTTG CAGTCTTGTG GAACAGTCTC CTCTCTAACA AACCGAGTGC TTCCACCCTC 
CCTCCACGAG ACAGACTCCA CTGTCAGCTG CAAGCTTTAA CATTCAGACT CCTTGAACAG 
GGCTCCAGCT CGTCCTCTTC CCCATCCAAA GTTCCTCTGT TCGTGGAGGA AGCAGTAGTG 
GAGTACGAAC GATCATGTGG AGGTTTTACT CAAGACGATG TGAGTTTTCT GACCTCTGAA 
TTGCGTGAGC TGTTCTCTGG TGCTTTCATC AATCCTCAGG ACTCTGCATC AACTTCTTCA 
CTGCTGGCTG TGTGGTGTGA GGTGGCATTT AAAGTGTCTA AACTGCTCTG CAAAAGTAGC 
TTTTCACCTG AGGCTGTTGA ACTCTTGCGA GGCACTCTGA AGGAGGTTAA TGGCCACGTT 
GGCCTGCGTT CGGCTCTGGG TCTGGCAGAC CGGGCAGTGC AGATGCAGTG TGTGTTAAGT 
TCTGGAGGTG AATGCAGTAA GAGCTTCACT GAATGTGCCC GTGCTCTCCG GATGTTGCCT 
TGTGCCATGT CCAGCTCAGA GTCTCATGCC CTGTTAGAGG CTTGTCAGCT GGTGGTTTGG 
ACGTTGGAAG CCAGTCAGTG TAAAGGAATG GATGTGACCA CTTTGCTGGC TTGTTTTTCC 
TTTTTGGAAG AATATCAGGA ATTTCTGCTT GCACAGCAAA AGAACTCGTT TTCGCTGCAG 
GTCCAATACT CTCTTTGTTT CAGTTTTTAC CAGGGCTTCA TCTGCACATA TGATAGTCTT 
CATGCTTCTC AGGTGTCTGT GGGCGAGACC TTGGACAGAG TGCTATTGTT CTGCCGGTCG 
ACCGCAGGAC GTATGCTTGC TGAACTGCGG ACACTCAATA ATGACAACTT GCTGCTTAAA 
GCAGTGTGTG CAGTGAATAA TGTGGTGTAC GAGCTGTTTA ACAGGAAGCT GTATGAAGGA 
GCCTTTGGAT TGTCGATGAT TGTGTGTCAG GAGCTCTGTA AGGACTGCCC GCCCTCTCTT 
CCTATGGATC GGGTCAACCG CTGTTTCATG CTGACAGTGC AGTGTAGTCG CCGCGCAGGC 
CATCTGGAGC AGGCTCTGGA CTGGATTGTT CGCTGGATTC AGGCTTTGGG CTCACAGATT 
TTGGATCATC TCACTGAACC TGTGTCGATG TGGGTCAAAA CTAAGTGTGA TGCCGCCAGA 
GCAGGCGAGG ATGACACACG CCTGAGGACT CTGAGGGACG GGTTAGGAGA ATCCTTAGTG 
GATGAGGAGG TTATGATGTG TTTGTTAGAG GAGGAGTTGC GTGTATATAA GGAGCAGACT 
GGAGACACAG CACAGGAACG CTACAACACA CTCTGCGACC TCTTGGAAAT CTGCCATGAA 
GAAACCCCAC ACACACTAAG AAGAGCTACG TATCTGTGTG AGATGGCACA GGTTGTGTGC 
TATCAGGACT TCAGCCAGCA GACTGACTGC TCAGCAGTTG ATTTTACCCA CGAGGCTTTG 
CGGTTGCTGG AAGCAGAACC AGAGACGGCA GAGAATACTG ATAGGCTGAA GGATGAGAAA 
GCCCACGCTT CCCTGTGGCT CTATATCTGC ACTCTTGAGT CTAACCTGCA GGAGGCTGTG 
GACACAGAGA AGCGTTTGTG TGCAGTGCAG GAGGAGAATA AAAGAGATGT GAATATGGAT 
CCTGTTCCCA CAAATGATCT GGAGTACGAG GACAAACAGA AATCACAAGA GAGCCAGTTA 
GTCTACGATG GACTACGCTT CAACCTGCTG GCTCACAGCA AGCAAAGTGA ACCTCTAGAT 
AAATGTTTGT CTCTATGGCG GAGTCTCCTC AAGGGGGCTG TGCCTGCAGT TAGAGACCCT 
AAACTCACAG CTTCTTCCAT GACTTTGATG GCTGCCCTAT ACACACTTAT GGGCAAGCAC 
CTGCAAGCTC TGGAGGGATA TCAGCTTGCT GCCACCCTTT TCCATGTACT TGGTGATGGC 
CAGAGTAGTG CCAATGCCTT CTGCCACTCT ACCAGGATCT TGCTTTACCT GGGTTCACCG 
CAGCTTGCAC AGGTGGACTT AGAAAAAGCA GAAAAGAGCT TGTCATCTGT CACCATCTCT 
GAGGGCACAT CTATTATCTC CATGCTGGCC ATGTTGCAAA AAGCACAGCT ACACTATGCT 
CTAGGACAGG TGGAGAGTGG CGTGTCCTGC CTGATGGAGG TGATAAAGGA ATCTGCTCAG 
CATCATTCTA AGAGCTGGTA CCTGCTCAGG GCTCGAGCAC TGCAGACAGC CAGTGAATAC 
TTGAGTCTGG ACACACTGGC TTTGAACTCA CAGCTACGCC AGAGCATCAT GCAGCAGGGT 
CTAAAGACTC CAGACACTGC TCAATATGAA GGGTTGAAGC TGTTGTGTAG CCTGGTGATG 
ATGTTATTGG GCAACGGGTT TTACGGAGCG CCTGGACCAA ACACTGACAC TCGCTTTGTG 
GATAAAGGAG ACAGTGTTGT GTTTAAGTGG CTGCTGCTGA GTGAAGTGCT GGTGTGCTCG 
GAGAGAATGG TGATCACCAG GAGCAGCAGC GGAGCCGTTC ATGAAGCTAA AGCTCAGTGT 
CTAGAGGCTC TCAAACTGGC CACCAAACTA CAGACACTCA GCCACTGTGC TGAGCTGTTG 
GTGCTGAAGG CAGAGCTGGA GCTGATGAAG GGTGCAACTG AAGCTAGTAG TTTAGACCTA 
GAGCAAGTTA GACATTTACT TGATCTCTGC ACAGATTTTG GCCAGGGACG ACAACAGAAA 
TCTGAAGTGA AAATCAAACC ACGCAAAGGT CGTCCCGCTG CCCCTTCTTC CTCTGGTCAC 
ATCTCTGAGG AAGAGGATGA TCTGAGTGGG ATCCTCAGCT CTCGTGCCCT TCCGAAGGAG 
CCTGTGGAGA ACATGGCTAG ATTAGGAAGC CAAGGAGCTT CTCCCCCTCT AAAGCCCAAA 
TGCCAGCATG GCCTCTCCTG TCTGACCCAT GAAGAGACCT GCTCTTGTCT CTGCTGCACT 
GAGCTCAGCG TGGCTCGAGT CAGCATCCAC TGGGCACTAT TACAGGCAGA TCTCCAGACA 
GATCCCGAAC GCTCTCTCAG ACTACGCCAG TCTGCCAGGA AACGCTGCCG CTATGTTGTA 
ACTAAACTTC AGAGAAGTTT GGCAGCACTT GTACACTCCA AAAAATCACC TAATGTCTGC 
TTGTCACTGC TTCAGGCTGA ACAGGGTAAA GTGCATCTGG GTGCTGTGCT TCAACTGCTC 
AGAACTGGAG ACAAAGGGAA AGCTGCCATC TTGTGGGAGG AGATAGAAGC AGGATTAGAG 
GCAGTGACAC CTAAAGGAGC ACTGACACCA GAACTTGGGC CTATCAGAGC TACTCTACTG 
GGGGCTAAAG CCGTAGCCTG CTGCTTGGCG TTGGCAATGA AGAAACAGTG TATACCCGAA 
GAGCTGTTTT CTTCATTGTG GCACTGGAAT CCACCAAAGG TTAAACCGCA ATTAAAATCT 
AAGCATCGAT CCAAGAGTCC GGACGCAGCT CCTCTAGAGG CAGGTGTTAA ACATAACCCT 
AAAGTTAAGA GTGGACTTGA GGAGAATGAA AGCAGAAAAA CCAAAGAGAC CACTGTCATT 
TCGAAGAAAG CTAAAGACTC TGTGCCCAAA ATTACCATCA CAAAATCCTC CATGGTTTTT 
AAAACGCCCA AAGCAACGAG AACAACCCGG CCCAAATCTG TCTCCACTAG CACTGGCATC 
AGTGACCTGA GAGCATTTGA CTTCAATAAT GAGGTGCCTG ACATTTCAGT CAACTTCACA 
CCATCATTAC ACCGATCTAC AAACCAAAGT GCGACTGCAA AGTCAAAAGC GTCCTCAAAG 
GGTTCGTTCC AGGTTTACAA AGATTCTTCT CCTGCTGCAG AGAAAGCTGT AGTTGTCCCG 
GCTGCTCCTA AACGAACAAA GCGTTCTCGC TTCAAGGTTG AGTTCAGCGA TGAAAGCGAC 
AACGATGCTG CTCCTCTTGC TGTAATGGAA AAGTCAGAGA AAAAGAAAAA CACCTTTAAA 
ACATCACGCA CCTTGAAACC AACCCTTAAC ACCATCCCTA GTGACACCAA ACTTGTGAGC 
TCCACAGTAG ATCCAGAACC CTCCAGACGA ACACGGACCA CTAAGAAAAG CACAGCTCTT 
CCCTCCATTG GTGGCCTCTC TTCTGAGGAA GAACTTGGAG TATCTTCACA GACACGTACG 
CGTAGAGGAC GACCAAAGAA GAGCACCAGC TCAGCAGACA CAGAGGAGCC AGAGAGGATG 
AGGATGATTA AGGAGGAAGA ACAGAGGGTT CTTTTGAATG TCAGCATGGA GGAGCTCAGA 
GGCTCTGACA CTGAGACAAA AGACACTGGA AGTCCAGATG CTGACTGTGA GGTCTTGAGG 
AGAGATCTAG GTGCAGATTT TGGGCGAGAG TGTTTCAGTG AGCTCAGGAG AGCTGGACAG 
AATGGATCAG GATCTCACAC AGCTCTGCCA CATGCAACTA CATCTCCTGA CTTGTCAGTA 
GAGACGGTCC AGTCATACCT GCGCTCCTCA TGGCTCCTCC TGCACCACTT CCCGTTTCCT 
TCTCTTTATC CTCACATCTG CTCATTACTC GCTCAGTCAT TCGGTCAAAC GGACCCCATC 
ATCACAGCAA TGCTGCATGC ACAGGCACTG GGAGTGTCCA CTCGCCACCA TATGACCCGT 
CACGTGGTTA ACCAGTTCAG AAAGTTGAAG AAATCCTGTA ACGATGTAGC AGAAGGTCTT 
GGAGCTCTGA GTCTGGAGGA AACATCTGGA GCAACGCAGT CAAAGAAACT CTCTGCGTTG 
GAGCAGATCT TTTCATTCAC AGCATCAAAC CCAATGCAGT TTCCACAGAC ACACTGTCAG 
CAGTTCACAC AGCAGCTGGA AGATCTACCT GCAGGAGTGA CCGTATGCTT GCTGTCACTG 
ACGGGGGTGC ATCCTGATGA AATTGGCAAC ACTATTCTTC TGACCCGACT GGAGCGCGGC 
TCTACCCCAA TCACAGTTCG AATCCCCACT GCAGACAGAG AGCAATCTGT TGCTGGCCTG 
TTGGAAGAAA TGGATGGAGT CTTGAAAGGA CAAAAGGAAG TGAGCACAGT GGCTGAGAAA 
TCACAGTGGT GGGACGGAAG GAAGGCTTTA GATGCCCGTG TGGAGAAAAT GCTGGAGGAG 
ATGGAAGAGG CTTTAGGAGT GTGGCGAACT CTTTTTCTGC CTTTGACCTC TGATCCTAAG 
CTTGATGTTC AGGTAAAGAG CCTCCAAAAG GCTCTTAAAT CAACAAAGAT CACACGGGAC 
ACTCTCAAGG TGATTCTATC AGCGTCTCCA CTGCTGTCCC TGCCAGACCT GCAGTGTCTT 
CTGGAAGGAA TGAATGTGCA GGACAAGGAC CTGCTGAAGC TCCTGCAGAG AGGCGTGGCT 
GAACTGAGAG GGAGGGAGGA GCCACAGGGA CACACTGTTC TGATTTTAGA CAAGTACTTG 
CAGAGGTTGC CGTGGGAGAA CATTGCTTGT CTGAAGTCTC AGTCGGTCAC TCGAATGCCC 
TCATTACACG CGGTGCTTGG ACACAGTCAT CTGAGAGAGG TGGATACAAG TTGTGTTCTA 
TCTTGTGGTG TGAACCCTAA GCAGGTTTAC TATGTGCTAA ACCCAGATGG AAACCTGCCC 
GACACTGAGA ATCGCTTCAA AGAGTGGTTT ACAGGTGAAC GAACATGGCA GGGGGTTTGT 
GGAAGGGCTC CTGATCCGGA GAAACTACAA GAGGCTGTGA CCACTAAAGA TCTCTACATA 
TACATCGGAC ATGGTGCAGG AGCCCGCTTT CTGGATGCGC AGAGAGTTTT GAAGGTGCCT 
GTGAGGACCG CAGCTCTTCT GTTCGGCTGC AGTAGTGCTG CTCTCAGTGT GCTTGGTCAG 
CAGGAGGGAA CGGGCATCAT CCTCAGCTAC CTGACCGCTG GATGTCCATT GGTGCTGGGT 
AACCTGTGGG ATGTGACAGA TCGTGACTTG GATCGATTCA CATCGGCTCT GCTCCAGTCT 
TGGCTCTCTG CTGGTTCTGG CTCTTCACTT CTTCAGCATT TGGCGAAATC ACGCAGCGCA 
ACTCATTTAA AACACATAAT CGGTGCTGCG CCCATTGCGT ATGGTTTACC GGTGCATATA 
CACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021332896.1
CDS	210..6755
Translation	MALKSLKTEDYVQQLSCVKDTIVLHNELKKHVDGGLGPYGRTMCDRIIRACNQRLGNGAVDAGLQERIVELVELAVQGFQLLEESGMQSTPFYLEKIVFHILQKVASLGAHGPACRLGQLVYRRLKCLSAEIDDFNVLVRNCFAVLWNSLLSNKPSASTLPPRDRLHCQLQALTFRLLEQGSSSSSSPSKVPLFVEEAVVEYERSCGGFTQDDVSFLTSELRELFSGAFINPQDSASTSSLLAVWCEVAFKVSKLLCKSSFSPEAVELLRGTLKEVNGHVGLRSALGLADRAVQMQCVLSSGGECSKSFTECARALRMLPCAMSSSESHALLEACQLVVWTLEASQCKGMDVTTLLACFSFLEEYQEFLLAQQKNSFSLQVQYSLCFSFYQGFICTYDSLHASQVSVGETLDRVLLFCRSTAGRMLAELRTLNNDNLLLKAVCAVNNVVYELFNRKLYEGAFGLSMIVCQELCKDCPPSLPMDRVNRCFMLTVQCSRRAGHLEQALDWIVRWIQALGSQILDHLTEPVSMWVKTKCDAARAGEDDTRLRTLRDGLGESLVDEEVMMCLLEEELRVYKEQTGDTAQERYNTLCDLLEICHEETPHTLRRATYLCEMAQVVCYQDFSQQTDCSAVDFTHEALRLLEAEPETAENTDRLKDEKAHASLWLYICTLESNLQEAVDTEKRLCAVQEENKRDVNMDPVPTNDLEYEDKQKSQESQLVYDGLRFNLLAHSKQSEPLDKCLSLWRSLLKGAVPAVRDPKLTASSMTLMAALYTLMGKHLQALEGYQLAATLFHVLGDGQSSANAFCHSTRILLYLGSPQLAQVDLEKAEKSLSSVTISEGTSIISMLAMLQKAQLHYALGQVESGVSCLMEVIKESAQHHSKSWYLLRARALQTASEYLSLDTLALNSQLRQSIMQQGLKTPDTAQYEGLKLLCSLVMMLLGNGFYGAPGPNTDTRFVDKGDSVVFKWLLLSEVLVCSERMVITRSSSGAVHEAKAQCLEALKLATKLQTLSHCAELLVLKAELELMKGATEASSLDLEQVRHLLDLCTDFGQGRQQKSEVKIKPRKGRPAAPSSSGHISEEEDDLSGILSSRALPKEPVENMARLGSQGASPPLKPKCQHGLSCLTHEETCSCLCCTELSVARVSIHWALLQADLQTDPERSLRLRQSARKRCRYVVTKLQRSLAALVHSKKSPNVCLSLLQAEQGKVHLGAVLQLLRTGDKGKAAILWEEIEAGLEAVTPKGALTPELGPIRATLLGAKAVACCLALAMKKQCIPEELFSSLWHWNPPKVKPQLKSKHRSKSPDAAPLEAGVKHNPKVKSGLEENESRKTKETTVISKKAKDSVPKITITKSSMVFKTPKATRTTRPKSVSTSTGISDLRAFDFNNEVPDISVNFTPSLHRSTNQSATAKSKASSKGSFQVYKDSSPAAEKAVVVPAAPKRTKRSRFKVEFSDESDNDAAPLAVMEKSEKKKNTFKTSRTLKPTLNTIPSDTKLVSSTVDPEPSRRTRTTKKSTALPSIGGLSSEEELGVSSQTRTRRGRPKKSTSSADTEEPERMRMIKEEEQRVLLNVSMEELRGSDTETKDTGSPDADCEVLRRDLGADFGRECFSELRRAGQNGSGSHTALPHATTSPDLSVETVQSYLRSSWLLLHHFPFPSLYPHICSLLAQSFGQTDPIITAMLHAQALGVSTRHHMTRHVVNQFRKLKKSCNDVAEGLGALSLEETSGATQSKKLSALEQIFSFTASNPMQFPQTHCQQFTQQLEDLPAGVTVCLLSLTGVHPDEIGNTILLTRLERGSTPITVRIPTADREQSVAGLLEEMDGVLKGQKEVSTVAEKSQWWDGRKALDARVEKMLEEMEEALGVWRTLFLPLTSDPKLDVQVKSLQKALKSTKITRDTLKVILSASPLLSLPDLQCLLEGMNVQDKDLLKLLQRGVAELRGREEPQGHTVLILDKYLQRLPWENIACLKSQSVTRMPSLHAVLGHSHLREVDTSCVLSCGVNPKQVYYVLNPDGNLPDTENRFKEWFTGERTWQGVCGRAPDPEKLQEAVTTKDLYIYIGHGAGARFLDAQRVLKVPVRTAALLFGCSSAALSVLGQQEGTGIILSYLTAGCPLVLGNLWDVTDRDLDRFTSALLQSWLSAGSGSSLLQHLAKSRSATHLKHIIGAAPIAYGLPVHIH

Target ORF information:

RefSeq Version	XM_021477221.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio extra spindle pole bodies like 1, separase (espl1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021477221.1

ORF Insert Sequence:

ATGGCATTAA AGAGCTTGAA AACAGAAGAT TACGTTCAGC AGTTATCCTG TGTCAAGGAC 
ACCATAGTCC TCCACAACGA ACTGAAGAAA CATGTGGATG GTGGCTTGGG TCCTTATGGT 
CGCACAATGT GTGATCGTAT CATCAGAGCC TGCAACCAGC GGCTGGGCAA CGGCGCTGTG 
GATGCTGGCC TTCAGGAGCG GATAGTGGAG CTGGTGGAAC TGGCTGTCCA AGGGTTTCAA 
CTGCTTGAAG AGTCCGGCAT GCAGAGCACC CCCTTTTATC TGGAGAAAAT AGTCTTTCAC 
ATCCTACAGA AAGTCGCAAG CCTTGGAGCT CATGGTCCTG CTTGTCGACT GGGTCAGCTC 
GTGTATAGAA GACTGAAATG TTTGTCTGCA GAGATTGATG ACTTTAATGT CCTGGTGCGG 
AATTGCTTTG CAGTCTTGTG GAACAGTCTC CTCTCTAACA AACCGAGTGC TTCCACCCTC 
CCTCCACGAG ACAGACTCCA CTGTCAGCTG CAAGCTTTAA CATTCAGACT CCTTGAACAG 
GGCTCCAGCT CGTCCTCTTC CCCATCCAAA GTTCCTCTGT TCGTGGAGGA AGCAGTAGTG 
GAGTACGAAC GATCATGTGG AGGTTTTACT CAAGACGATG TGAGTTTTCT GACCTCTGAA 
TTGCGTGAGC TGTTCTCTGG TGCTTTCATC AATCCTCAGG ACTCTGCATC AACTTCTTCA 
CTGCTGGCTG TGTGGTGTGA GGTGGCATTT AAAGTGTCTA AACTGCTCTG CAAAAGTAGC 
TTTTCACCTG AGGCTGTTGA ACTCTTGCGA GGCACTCTGA AGGAGGTTAA TGGCCACGTT 
GGCCTGCGTT CGGCTCTGGG TCTGGCAGAC CGGGCAGTGC AGATGCAGTG TGTGTTAAGT 
TCTGGAGGTG AATGCAGTAA GAGCTTCACT GAATGTGCCC GTGCTCTCCG GATGTTGCCT 
TGTGCCATGT CCAGCTCAGA GTCTCATGCC CTGTTAGAGG CTTGTCAGCT GGTGGTTTGG 
ACGTTGGAAG CCAGTCAGTG TAAAGGAATG GATGTGACCA CTTTGCTGGC TTGTTTTTCC 
TTTTTGGAAG AATATCAGGA ATTTCTGCTT GCACAGCAAA AGAACTCGTT TTCGCTGCAG 
GTCCAATACT CTCTTTGTTT CAGTTTTTAC CAGGGCTTCA TCTGCACATA TGATAGTCTT 
CATGCTTCTC AGGTGTCTGT GGGCGAGACC TTGGACAGAG TGCTATTGTT CTGCCGGTCG 
ACCGCAGGAC GTATGCTTGC TGAACTGCGG ACACTCAATA ATGACAACTT GCTGCTTAAA 
GCAGTGTGTG CAGTGAATAA TGTGGTGTAC GAGCTGTTTA ACAGGAAGCT GTATGAAGGA 
GCCTTTGGAT TGTCGATGAT TGTGTGTCAG GAGCTCTGTA AGGACTGCCC GCCCTCTCTT 
CCTATGGATC GGGTCAACCG CTGTTTCATG CTGACAGTGC AGTGTAGTCG CCGCGCAGGC 
CATCTGGAGC AGGCTCTGGA CTGGATTGTT CGCTGGATTC AGGCTTTGGG CTCACAGATT 
TTGGATCATC TCACTGAACC TGTGTCGATG TGGGTCAAAA CTAAGTGTGA TGCCGCCAGA 
GCAGGCGAGG ATGACACACG CCTGAGGACT CTGAGGGACG GGTTAGGAGA ATCCTTAGTG 
GATGAGGAGG TTATGATGTG TTTGTTAGAG GAGGAGTTGC GTGTATATAA GGAGCAGACT 
GGAGACACAG CACAGGAACG CTACAACACA CTCTGCGACC TCTTGGAAAT CTGCCATGAA 
GAAACCCCAC ACACACTAAG AAGAGCTACG TATCTGTGTG AGATGGCACA GGTTGTGTGC 
TATCAGGACT TCAGCCAGCA GACTGACTGC TCAGCAGTTG ATTTTACCCA CGAGGCTTTG 
CGGTTGCTGG AAGCAGAACC AGAGACGGCA GAGAATACTG ATAGGCTGAA GGATGAGAAA 
GCCCACGCTT CCCTGTGGCT CTATATCTGC ACTCTTGAGT CTAACCTGCA GGAGGCTGTG 
GACACAGAGA AGCGTTTGTG TGCAGTGCAG GAGGAGAATA AAAGAGATGT GAATATGGAT 
CCTGTTCCCA CAAATGATCT GGAGTACGAG GACAAACAGA AATCACAAGA GAGCCAGTTA 
GTCTACGATG GACTACGCTT CAACCTGCTG GCTCACAGCA AGCAAAGTGA ACCTCTAGAT 
AAATGTTTGT CTCTATGGCG GAGTCTCCTC AAGGGGGCTG TGCCTGCAGT TAGAGACCCT 
AAACTCACAG CTTCTTCCAT GACTTTGATG GCTGCCCTAT ACACACTTAT GGGCAAGCAC 
CTGCAAGCTC TGGAGGGATA TCAGCTTGCT GCCACCCTTT TCCATGTACT TGGTGATGGC 
CAGAGTAGTG CCAATGCCTT CTGCCACTCT ACCAGGATCT TGCTTTACCT GGGTTCACCG 
CAGCTTGCAC AGGTGGACTT AGAAAAAGCA GAAAAGAGCT TGTCATCTGT CACCATCTCT 
GAGGGCACAT CTATTATCTC CATGCTGGCC ATGTTGCAAA AAGCACAGCT ACACTATGCT 
CTAGGACAGG TGGAGAGTGG CGTGTCCTGC CTGATGGAGG TGATAAAGGA ATCTGCTCAG 
CATCATTCTA AGAGCTGGTA CCTGCTCAGG GCTCGAGCAC TGCAGACAGC CAGTGAATAC 
TTGAGTCTGG ACACACTGGC TTTGAACTCA CAGCTACGCC AGAGCATCAT GCAGCAGGGT 
CTAAAGACTC CAGACACTGC TCAATATGAA GGGTTGAAGC TGTTGTGTAG CCTGGTGATG 
ATGTTATTGG GCAACGGGTT TTACGGAGCG CCTGGACCAA ACACTGACAC TCGCTTTGTG 
GATAAAGGAG ACAGTGTTGT GTTTAAGTGG CTGCTGCTGA GTGAAGTGCT GGTGTGCTCG 
GAGAGAATGG TGATCACCAG GAGCAGCAGC GGAGCCGTTC ATGAAGCTAA AGCTCAGTGT 
CTAGAGGCTC TCAAACTGGC CACCAAACTA CAGACACTCA GCCACTGTGC TGAGCTGTTG 
GTGCTGAAGG CAGAGCTGGA GCTGATGAAG GGTGCAACTG AAGCTAGTAG TTTAGACCTA 
GAGCAAGTTA GACATTTACT TGATCTCTGC ACAGATTTTG GCCAGGGACG ACAACAGAAA 
TCTGAAGTGA AAATCAAACC ACGCAAAGGT CGTCCCGCTG CCCCTTCTTC CTCTGGTCAC 
ATCTCTGAGG AAGAGGATGA TCTGAGTGGG ATCCTCAGCT CTCGTGCCCT TCCGAAGGAG 
CCTGTGGAGA ACATGGCTAG ATTAGGAAGC CAAGGAGCTT CTCCCCCTCT AAAGCCCAAA 
TGCCAGCATG GCCTCTCCTG TCTGACCCAT GAAGAGACCT GCTCTTGTCT CTGCTGCACT 
GAGCTCAGCG TGGCTCGAGT CAGCATCCAC TGGGCACTAT TACAGGCAGA TCTCCAGACA 
GATCCCGAAC GCTCTCTCAG ACTACGCCAG TCTGCCAGGA AACGCTGCCG CTATGTTGTA 
ACTAAACTTC AGAGAAGTTT GGCAGCACTT GTACACTCCA AAAAATCACC TAATGTCTGC 
TTGTCACTGC TTCAGGCTGA ACAGGGTAAA GTGCATCTGG GTGCTGTGCT TCAACTGCTC 
AGAACTGGAG ACAAAGGGAA AGCTGCCATC TTGTGGGAGG AGATAGAAGC AGGATTAGAG 
GCAGTGACAC CTAAAGGAGC ACTGACACCA GAACTTGGGC CTATCAGAGC TACTCTACTG 
GGGGCTAAAG CCGTAGCCTG CTGCTTGGCG TTGGCAATGA AGAAACAGTG TATACCCGAA 
GAGCTGTTTT CTTCATTGTG GCACTGGAAT CCACCAAAGG TTAAACCGCA ATTAAAATCT 
AAGCATCGAT CCAAGAGTCC GGACGCAGCT CCTCTAGAGG CAGGTGTTAA ACATAACCCT 
AAAGTTAAGA GTGGACTTGA GGAGAATGAA AGCAGAAAAA CCAAAGAGAC CACTGTCATT 
TCGAAGAAAG CTAAAGACTC TGTGCCCAAA ATTACCATCA CAAAATCCTC CATGGTTTTT 
AAAACGCCCA AAGCAACGAG AACAACCCGG CCCAAATCTG TCTCCACTAG CACTGGCATC 
AGTGACCTGA GAGCATTTGA CTTCAATAAT GAGGTGCCTG ACATTTCAGT CAACTTCACA 
CCATCATTAC ACCGATCTAC AAACCAAAGT GCGACTGCAA AGTCAAAAGC GTCCTCAAAG 
GGTTCGTTCC AGGTTTACAA AGATTCTTCT CCTGCTGCAG AGAAAGCTGT AGTTGTCCCG 
GCTGCTCCTA AACGAACAAA GCGTTCTCGC TTCAAGGTTG AGTTCAGCGA TGAAAGCGAC 
AACGATGCTG CTCCTCTTGC TGTAATGGAA AAGTCAGAGA AAAAGAAAAA CACCTTTAAA 
ACATCACGCA CCTTGAAACC AACCCTTAAC ACCATCCCTA GTGACACCAA ACTTGTGAGC 
TCCACAGTAG ATCCAGAACC CTCCAGACGA ACACGGACCA CTAAGAAAAG CACAGCTCTT 
CCCTCCATTG GTGGCCTCTC TTCTGAGGAA GAACTTGGAG TATCTTCACA GACACGTACG 
CGTAGAGGAC GACCAAAGAA GAGCACCAGC TCAGCAGACA CAGAGGAGCC AGAGAGGATG 
AGGATGATTA AGGAGGAAGA ACAGAGGGTT CTTTTGAATG TCAGCATGGA GGAGCTCAGA 
GGCTCTGACA CTGAGACAAA AGACACTGGA AGTCCAGATG CTGACTGTGA GGTCTTGAGG 
AGAGATCTAG GTGCAGATTT TGGGCGAGAG TGTTTCAGTG AGCTCAGGAG AGCTGGACAG 
AATGGATCAG GATCTCACAC AGCTCTGCCA CATGCAACTA CATCTCCTGA CTTGTCAGTA 
GAGACGGTCC AGTCATACCT GCGCTCCTCA TGGCTCCTCC TGCACCACTT CCCGTTTCCT 
TCTCTTTATC CTCACATCTG CTCATTACTC GCTCAGTCAT TCGGTCAAAC GGACCCCATC 
ATCACAGCAA TGCTGCATGC ACAGGCACTG GGAGTGTCCA CTCGCCACCA TATGACCCGT 
CACGTGGTTA ACCAGTTCAG AAAGTTGAAG AAATCCTGTA ACGATGTAGC AGAAGGTCTT 
GGAGCTCTGA GTCTGGAGGA AACATCTGGA GCAACGCAGT CAAAGAAACT CTCTGCGTTG 
GAGCAGATCT TTTCATTCAC AGCATCAAAC CCAATGCAGT TTCCACAGAC ACACTGTCAG 
CAGTTCACAC AGCAGCTGGA AGATCTACCT GCAGGAGTGA CCGTATGCTT GCTGTCACTG 
ACGGGGGTGC ATCCTGATGA AATTGGCAAC ACTATTCTTC TGACCCGACT GGAGCGCGGC 
TCTACCCCAA TCACAGTTCG AATCCCCACT GCAGACAGAG AGCAATCTGT TGCTGGCCTG 
TTGGAAGAAA TGGATGGAGT CTTGAAAGGA CAAAAGGAAG TGAGCACAGT GGCTGAGAAA 
TCACAGTGGT GGGACGGAAG GAAGGCTTTA GATGCCCGTG TGGAGAAAAT GCTGGAGGAG 
ATGGAAGAGG CTTTAGGAGT GTGGCGAACT CTTTTTCTGC CTTTGACCTC TGATCCTAAG 
CTTGATGTTC AGGTAAAGAG CCTCCAAAAG GCTCTTAAAT CAACAAAGAT CACACGGGAC 
ACTCTCAAGG TGATTCTATC AGCGTCTCCA CTGCTGTCCC TGCCAGACCT GCAGTGTCTT 
CTGGAAGGAA TGAATGTGCA GGACAAGGAC CTGCTGAAGC TCCTGCAGAG AGGCGTGGCT 
GAACTGAGAG GGAGGGAGGA GCCACAGGGA CACACTGTTC TGATTTTAGA CAAGTACTTG 
CAGAGGTTGC CGTGGGAGAA CATTGCTTGT CTGAAGTCTC AGTCGGTCAC TCGAATGCCC 
TCATTACACG CGGTGCTTGG ACACAGTCAT CTGAGAGAGG TGGATACAAG TTGTGTTCTA 
TCTTGTGGTG TGAACCCTAA GCAGGTTTAC TATGTGCTAA ACCCAGATGG AAACCTGCCC 
GACACTGAGA ATCGCTTCAA AGAGTGGTTT ACAGGTGAAC GAACATGGCA GGGGGTTTGT 
GGAAGGGCTC CTGATCCGGA GAAACTACAA GAGGCTGTGA CCACTAAAGA TCTCTACATA 
TACATCGGAC ATGGTGCAGG AGCCCGCTTT CTGGATGCGC AGAGAGTTTT GAAGGTGCCT 
GTGAGGACCG CAGCTCTTCT GTTCGGCTGC AGTAGTGCTG CTCTCAGTGT GCTTGGTCAG 
CAGGAGGGAA CGGGCATCAT CCTCAGCTAC CTGACCGCTG GATGTCCATT GGTGCTGGGT 
AACCTGTGGG ATGTGACAGA TCGTGACTTG GATCGATTCA CATCGGCTCT GCTCCAGTCT 
TGGCTCTCTG CTGGTTCTGG CTCTTCACTT CTTCAGCATT TGGCGAAATC ACGCAGCGCA 
ACTCATTTAA AACACATAAT CGGTGCTGCG CCCATTGCGT ATGGTTTACC GGTGCATATA 
CACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

espl1 ( XM_005166094.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005166151.1 Danio rerio extra spindle pole bodies like 1, separase (espl1), transcript variant X2, mRNA.

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CloneID	ODa12113
Clone ID Related Accession (Same CDS sequence)	XM_005166094.3 , XM_021477221.1 , XM_005166094.4
Accession Version	XM_005166094.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6546bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	separin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395382.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005166094.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCATTAA AGAGCTTGAA AACAGAAGAT TACGTTCAGC AGTTATCCTG TGTCAAGGAC 
ACCATAGTCC TCCACAACGA ACTGAAGAAA CATGTGGATG GTGGCTTGGG TCCTTATGGT 
CGCACAATGT GTGATCGTAT CATCAGAGCC TGCAACCAGC GGCTGGGCAA CGGCGCTGTG 
GATGCTGGCC TTCAGGAGCG GATAGTGGAG CTGGTGGAAC TGGCTGTCCA AGGGTTTCAA 
CTGCTTGAAG AGTCCGGCAT GCAGAGCACC CCCTTTTATC TGGAGAAAAT AGTCTTTCAC 
ATCCTACAGA AAGTCGCAAG CCTTGGAGCT CATGGTCCTG CTTGTCGACT GGGTCAGCTC 
GTGTATAGAA GACTGAAATG TTTGTCTGCA GAGATTGATG ACTTTAATGT CCTGGTGCGG 
AATTGCTTTG CAGTCTTGTG GAACAGTCTC CTCTCTAACA AACCGAGTGC TTCCACCCTC 
CCTCCACGAG ACAGACTCCA CTGTCAGCTG CAAGCTTTAA CATTCAGACT CCTTGAACAG 
GGCTCCAGCT CGTCCTCTTC CCCATCCAAA GTTCCTCTGT TCGTGGAGGA AGCAGTAGTG 
GAGTACGAAC GATCATGTGG AGGTTTTACT CAAGACGATG TGAGTTTTCT GACCTCTGAA 
TTGCGTGAGC TGTTCTCTGG TGCTTTCATC AATCCTCAGG ACTCTGCATC AACTTCTTCA 
CTGCTGGCTG TGTGGTGTGA GGTGGCATTT AAAGTGTCTA AACTGCTCTG CAAAAGTAGC 
TTTTCACCTG AGGCTGTTGA ACTCTTGCGA GGCACTCTGA AGGAGGTTAA TGGCCACGTT 
GGCCTGCGTT CGGCTCTGGG TCTGGCAGAC CGGGCAGTGC AGATGCAGTG TGTGTTAAGT 
TCTGGAGGTG AATGCAGTAA GAGCTTCACT GAATGTGCCC GTGCTCTCCG GATGTTGCCT 
TGTGCCATGT CCAGCTCAGA GTCTCATGCC CTGTTAGAGG CTTGTCAGCT GGTGGTTTGG 
ACGTTGGAAG CCAGTCAGTG TAAAGGAATG GATGTGACCA CTTTGCTGGC TTGTTTTTCC 
TTTTTGGAAG AATATCAGGA ATTTCTGCTT GCACAGCAAA AGAACTCGTT TTCGCTGCAG 
GTCCAATACT CTCTTTGTTT CAGTTTTTAC CAGGGCTTCA TCTGCACATA TGATAGTCTT 
CATGCTTCTC AGGTGTCTGT GGGCGAGACC TTGGACAGAG TGCTATTGTT CTGCCGGTCG 
ACCGCAGGAC GTATGCTTGC TGAACTGCGG ACACTCAATA ATGACAACTT GCTGCTTAAA 
GCAGTGTGTG CAGTGAATAA TGTGGTGTAC GAGCTGTTTA ACAGGAAGCT GTATGAAGGA 
GCCTTTGGAT TGTCGATGAT TGTGTGTCAG GAGCTCTGTA AGGACTGCCC GCCCTCTCTT 
CCTATGGATC GGGTCAACCG CTGTTTCATG CTGACAGTGC AGTGTAGTCG CCGCGCAGGC 
CATCTGGAGC AGGCTCTGGA CTGGATTGTT CGCTGGATTC AGGCTTTGGG CTCACAGATT 
TTGGATCATC TCACTGAACC TGTGTCGATG TGGGTCAAAA CTAAGTGTGA TGCCGCCAGA 
GCAGGCGAGG ATGACACACG CCTGAGGACT CTGAGGGACG GGTTAGGAGA ATCCTTAGTG 
GATGAGGAGG TTATGATGTG TTTGTTAGAG GAGGAGTTGC GTGTATATAA GGAGCAGACT 
GGAGACACAG CACAGGAACG CTACAACACA CTCTGCGACC TCTTGGAAAT CTGCCATGAA 
GAAACCCCAC ACACACTAAG AAGAGCTACG TATCTGTGTG AGATGGCACA GGTTGTGTGC 
TATCAGGACT TCAGCCAGCA GACTGACTGC TCAGCAGTTG ATTTTACCCA CGAGGCTTTG 
CGGTTGCTGG AAGCAGAACC AGAGACGGCA GAGAATACTG ATAGGCTGAA GGATGAGAAA 
GCCCACGCTT CCCTGTGGCT CTATATCTGC ACTCTTGAGT CTAACCTGCA GGAGGCTGTG 
GACACAGAGA AGCGTTTGTG TGCAGTGCAG GAGGAGAATA AAAGAGATGT GAATATGGAT 
CCTGTTCCCA CAAATGATCT GGAGTACGAG GACAAACAGA AATCACAAGA GAGCCAGTTA 
GTCTACGATG GACTACGCTT CAACCTGCTG GCTCACAGCA AGCAAAGTGA ACCTCTAGAT 
AAATGTTTGT CTCTATGGCG GAGTCTCCTC AAGGGGGCTG TGCCTGCAGT TAGAGACCCT 
AAACTCACAG CTTCTTCCAT GACTTTGATG GCTGCCCTAT ACACACTTAT GGGCAAGCAC 
CTGCAAGCTC TGGAGGGATA TCAGCTTGCT GCCACCCTTT TCCATGTACT TGGTGATGGC 
CAGAGTAGTG CCAATGCCTT CTGCCACTCT ACCAGGATCT TGCTTTACCT GGGTTCACCG 
CAGCTTGCAC AGGTGGACTT AGAAAAAGCA GAAAAGAGCT TGTCATCTGT CACCATCTCT 
GAGGGCACAT CTATTATCTC CATGCTGGCC ATGTTGCAAA AAGCACAGCT ACACTATGCT 
CTAGGACAGG TGGAGAGTGG CGTGTCCTGC CTGATGGAGG TGATAAAGGA ATCTGCTCAG 
CATCATTCTA AGAGCTGGTA CCTGCTCAGG GCTCGAGCAC TGCAGACAGC CAGTGAATAC 
TTGAGTCTGG ACACACTGGC TTTGAACTCA CAGCTACGCC AGAGCATCAT GCAGCAGGGT 
CTAAAGACTC CAGACACTGC TCAATATGAA GGGTTGAAGC TGTTGTGTAG CCTGGTGATG 
ATGTTATTGG GCAACGGGTT TTACGGAGCG CCTGGACCAA ACACTGACAC TCGCTTTGTG 
GATAAAGGAG ACAGTGTTGT GTTTAAGTGG CTGCTGCTGA GTGAAGTGCT GGTGTGCTCG 
GAGAGAATGG TGATCACCAG GAGCAGCAGC GGAGCCGTTC ATGAAGCTAA AGCTCAGTGT 
CTAGAGGCTC TCAAACTGGC CACCAAACTA CAGACACTCA GCCACTGTGC TGAGCTGTTG 
GTGCTGAAGG CAGAGCTGGA GCTGATGAAG GGTGCAACTG AAGCTAGTAG TTTAGACCTA 
GAGCAAGTTA GACATTTACT TGATCTCTGC ACAGATTTTG GCCAGGGACG ACAACAGAAA 
TCTGAAGTGA AAATCAAACC ACGCAAAGGT CGTCCCGCTG CCCCTTCTTC CTCTGGTCAC 
ATCTCTGAGG AAGAGGATGA TCTGAGTGGG ATCCTCAGCT CTCGTGCCCT TCCGAAGGAG 
CCTGTGGAGA ACATGGCTAG ATTAGGAAGC CAAGGAGCTT CTCCCCCTCT AAAGCCCAAA 
TGCCAGCATG GCCTCTCCTG TCTGACCCAT GAAGAGACCT GCTCTTGTCT CTGCTGCACT 
GAGCTCAGCG TGGCTCGAGT CAGCATCCAC TGGGCACTAT TACAGGCAGA TCTCCAGACA 
GATCCCGAAC GCTCTCTCAG ACTACGCCAG TCTGCCAGGA AACGCTGCCG CTATGTTGTA 
ACTAAACTTC AGAGAAGTTT GGCAGCACTT GTACACTCCA AAAAATCACC TAATGTCTGC 
TTGTCACTGC TTCAGGCTGA ACAGGGTAAA GTGCATCTGG GTGCTGTGCT TCAACTGCTC 
AGAACTGGAG ACAAAGGGAA AGCTGCCATC TTGTGGGAGG AGATAGAAGC AGGATTAGAG 
GCAGTGACAC CTAAAGGAGC ACTGACACCA GAACTTGGGC CTATCAGAGC TACTCTACTG 
GGGGCTAAAG CCGTAGCCTG CTGCTTGGCG TTGGCAATGA AGAAACAGTG TATACCCGAA 
GAGCTGTTTT CTTCATTGTG GCACTGGAAT CCACCAAAGG TTAAACCGCA ATTAAAATCT 
AAGCATCGAT CCAAGAGTCC GGACGCAGCT CCTCTAGAGG CAGGTGTTAA ACATAACCCT 
AAAGTTAAGA GTGGACTTGA GGAGAATGAA AGCAGAAAAA CCAAAGAGAC CACTGTCATT 
TCGAAGAAAG CTAAAGACTC TGTGCCCAAA ATTACCATCA CAAAATCCTC CATGGTTTTT 
AAAACGCCCA AAGCAACGAG AACAACCCGG CCCAAATCTG TCTCCACTAG CACTGGCATC 
AGTGACCTGA GAGCATTTGA CTTCAATAAT GAGGTGCCTG ACATTTCAGT CAACTTCACA 
CCATCATTAC ACCGATCTAC AAACCAAAGT GCGACTGCAA AGTCAAAAGC GTCCTCAAAG 
GGTTCGTTCC AGGTTTACAA AGATTCTTCT CCTGCTGCAG AGAAAGCTGT AGTTGTCCCG 
GCTGCTCCTA AACGAACAAA GCGTTCTCGC TTCAAGGTTG AGTTCAGCGA TGAAAGCGAC 
AACGATGCTG CTCCTCTTGC TGTAATGGAA AAGTCAGAGA AAAAGAAAAA CACCTTTAAA 
ACATCACGCA CCTTGAAACC AACCCTTAAC ACCATCCCTA GTGACACCAA ACTTGTGAGC 
TCCACAGTAG ATCCAGAACC CTCCAGACGA ACACGGACCA CTAAGAAAAG CACAGCTCTT 
CCCTCCATTG GTGGCCTCTC TTCTGAGGAA GAACTTGGAG TATCTTCACA GACACGTACG 
CGTAGAGGAC GACCAAAGAA GAGCACCAGC TCAGCAGACA CAGAGGAGCC AGAGAGGATG 
AGGATGATTA AGGAGGAAGA ACAGAGGGTT CTTTTGAATG TCAGCATGGA GGAGCTCAGA 
GGCTCTGACA CTGAGACAAA AGACACTGGA AGTCCAGATG CTGACTGTGA GGTCTTGAGG 
AGAGATCTAG GTGCAGATTT TGGGCGAGAG TGTTTCAGTG AGCTCAGGAG AGCTGGACAG 
AATGGATCAG GATCTCACAC AGCTCTGCCA CATGCAACTA CATCTCCTGA CTTGTCAGTA 
GAGACGGTCC AGTCATACCT GCGCTCCTCA TGGCTCCTCC TGCACCACTT CCCGTTTCCT 
TCTCTTTATC CTCACATCTG CTCATTACTC GCTCAGTCAT TCGGTCAAAC GGACCCCATC 
ATCACAGCAA TGCTGCATGC ACAGGCACTG GGAGTGTCCA CTCGCCACCA TATGACCCGT 
CACGTGGTTA ACCAGTTCAG AAAGTTGAAG AAATCCTGTA ACGATGTAGC AGAAGGTCTT 
GGAGCTCTGA GTCTGGAGGA AACATCTGGA GCAACGCAGT CAAAGAAACT CTCTGCGTTG 
GAGCAGATCT TTTCATTCAC AGCATCAAAC CCAATGCAGT TTCCACAGAC ACACTGTCAG 
CAGTTCACAC AGCAGCTGGA AGATCTACCT GCAGGAGTGA CCGTATGCTT GCTGTCACTG 
ACGGGGGTGC ATCCTGATGA AATTGGCAAC ACTATTCTTC TGACCCGACT GGAGCGCGGC 
TCTACCCCAA TCACAGTTCG AATCCCCACT GCAGACAGAG AGCAATCTGT TGCTGGCCTG 
TTGGAAGAAA TGGATGGAGT CTTGAAAGGA CAAAAGGAAG TGAGCACAGT GGCTGAGAAA 
TCACAGTGGT GGGACGGAAG GAAGGCTTTA GATGCCCGTG TGGAGAAAAT GCTGGAGGAG 
ATGGAAGAGG CTTTAGGAGT GTGGCGAACT CTTTTTCTGC CTTTGACCTC TGATCCTAAG 
CTTGATGTTC AGGTAAAGAG CCTCCAAAAG GCTCTTAAAT CAACAAAGAT CACACGGGAC 
ACTCTCAAGG TGATTCTATC AGCGTCTCCA CTGCTGTCCC TGCCAGACCT GCAGTGTCTT 
CTGGAAGGAA TGAATGTGCA GGACAAGGAC CTGCTGAAGC TCCTGCAGAG AGGCGTGGCT 
GAACTGAGAG GGAGGGAGGA GCCACAGGGA CACACTGTTC TGATTTTAGA CAAGTACTTG 
CAGAGGTTGC CGTGGGAGAA CATTGCTTGT CTGAAGTCTC AGTCGGTCAC TCGAATGCCC 
TCATTACACG CGGTGCTTGG ACACAGTCAT CTGAGAGAGG TGGATACAAG TTGTGTTCTA 
TCTTGTGGTG TGAACCCTAA GCAGGTTTAC TATGTGCTAA ACCCAGATGG AAACCTGCCC 
GACACTGAGA ATCGCTTCAA AGAGTGGTTT ACAGGTGAAC GAACATGGCA GGGGGTTTGT 
GGAAGGGCTC CTGATCCGGA GAAACTACAA GAGGCTGTGA CCACTAAAGA TCTCTACATA 
TACATCGGAC ATGGTGCAGG AGCCCGCTTT CTGGATGCGC AGAGAGTTTT GAAGGTGCCT 
GTGAGGACCG CAGCTCTTCT GTTCGGCTGC AGTAGTGCTG CTCTCAGTGT GCTTGGTCAG 
CAGGAGGGAA CGGGCATCAT CCTCAGCTAC CTGACCGCTG GATGTCCATT GGTGCTGGGT 
AACCTGTGGG ATGTGACAGA TCGTGACTTG GATCGATTCA CATCGGCTCT GCTCCAGTCT 
TGGCTCTCTG CTGGTTCTGG CTCTTCACTT CTTCAGCATT TGGCGAAATC ACGCAGCGCA 
ACTCATTTAA AACACATAAT CGGTGCTGCG CCCATTGCGT ATGGTTTACC GGTGCATATA 
CACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005166151.1
CDS	210..6755
Translation	MALKSLKTEDYVQQLSCVKDTIVLHNELKKHVDGGLGPYGRTMCDRIIRACNQRLGNGAVDAGLQERIVELVELAVQGFQLLEESGMQSTPFYLEKIVFHILQKVASLGAHGPACRLGQLVYRRLKCLSAEIDDFNVLVRNCFAVLWNSLLSNKPSASTLPPRDRLHCQLQALTFRLLEQGSSSSSSPSKVPLFVEEAVVEYERSCGGFTQDDVSFLTSELRELFSGAFINPQDSASTSSLLAVWCEVAFKVSKLLCKSSFSPEAVELLRGTLKEVNGHVGLRSALGLADRAVQMQCVLSSGGECSKSFTECARALRMLPCAMSSSESHALLEACQLVVWTLEASQCKGMDVTTLLACFSFLEEYQEFLLAQQKNSFSLQVQYSLCFSFYQGFICTYDSLHASQVSVGETLDRVLLFCRSTAGRMLAELRTLNNDNLLLKAVCAVNNVVYELFNRKLYEGAFGLSMIVCQELCKDCPPSLPMDRVNRCFMLTVQCSRRAGHLEQALDWIVRWIQALGSQILDHLTEPVSMWVKTKCDAARAGEDDTRLRTLRDGLGESLVDEEVMMCLLEEELRVYKEQTGDTAQERYNTLCDLLEICHEETPHTLRRATYLCEMAQVVCYQDFSQQTDCSAVDFTHEALRLLEAEPETAENTDRLKDEKAHASLWLYICTLESNLQEAVDTEKRLCAVQEENKRDVNMDPVPTNDLEYEDKQKSQESQLVYDGLRFNLLAHSKQSEPLDKCLSLWRSLLKGAVPAVRDPKLTASSMTLMAALYTLMGKHLQALEGYQLAATLFHVLGDGQSSANAFCHSTRILLYLGSPQLAQVDLEKAEKSLSSVTISEGTSIISMLAMLQKAQLHYALGQVESGVSCLMEVIKESAQHHSKSWYLLRARALQTASEYLSLDTLALNSQLRQSIMQQGLKTPDTAQYEGLKLLCSLVMMLLGNGFYGAPGPNTDTRFVDKGDSVVFKWLLLSEVLVCSERMVITRSSSGAVHEAKAQCLEALKLATKLQTLSHCAELLVLKAELELMKGATEASSLDLEQVRHLLDLCTDFGQGRQQKSEVKIKPRKGRPAAPSSSGHISEEEDDLSGILSSRALPKEPVENMARLGSQGASPPLKPKCQHGLSCLTHEETCSCLCCTELSVARVSIHWALLQADLQTDPERSLRLRQSARKRCRYVVTKLQRSLAALVHSKKSPNVCLSLLQAEQGKVHLGAVLQLLRTGDKGKAAILWEEIEAGLEAVTPKGALTPELGPIRATLLGAKAVACCLALAMKKQCIPEELFSSLWHWNPPKVKPQLKSKHRSKSPDAAPLEAGVKHNPKVKSGLEENESRKTKETTVISKKAKDSVPKITITKSSMVFKTPKATRTTRPKSVSTSTGISDLRAFDFNNEVPDISVNFTPSLHRSTNQSATAKSKASSKGSFQVYKDSSPAAEKAVVVPAAPKRTKRSRFKVEFSDESDNDAAPLAVMEKSEKKKNTFKTSRTLKPTLNTIPSDTKLVSSTVDPEPSRRTRTTKKSTALPSIGGLSSEEELGVSSQTRTRRGRPKKSTSSADTEEPERMRMIKEEEQRVLLNVSMEELRGSDTETKDTGSPDADCEVLRRDLGADFGRECFSELRRAGQNGSGSHTALPHATTSPDLSVETVQSYLRSSWLLLHHFPFPSLYPHICSLLAQSFGQTDPIITAMLHAQALGVSTRHHMTRHVVNQFRKLKKSCNDVAEGLGALSLEETSGATQSKKLSALEQIFSFTASNPMQFPQTHCQQFTQQLEDLPAGVTVCLLSLTGVHPDEIGNTILLTRLERGSTPITVRIPTADREQSVAGLLEEMDGVLKGQKEVSTVAEKSQWWDGRKALDARVEKMLEEMEEALGVWRTLFLPLTSDPKLDVQVKSLQKALKSTKITRDTLKVILSASPLLSLPDLQCLLEGMNVQDKDLLKLLQRGVAELRGREEPQGHTVLILDKYLQRLPWENIACLKSQSVTRMPSLHAVLGHSHLREVDTSCVLSCGVNPKQVYYVLNPDGNLPDTENRFKEWFTGERTWQGVCGRAPDPEKLQEAVTTKDLYIYIGHGAGARFLDAQRVLKVPVRTAALLFGCSSAALSVLGQQEGTGIILSYLTAGCPLVLGNLWDVTDRDLDRFTSALLQSWLSAGSGSSLLQHLAKSRSATHLKHIIGAAPIAYGLPVHIH

Target ORF information:

RefSeq Version	XM_005166094.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio extra spindle pole bodies like 1, separase (espl1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005166094.4

ORF Insert Sequence:

ATGGCATTAA AGAGCTTGAA AACAGAAGAT TACGTTCAGC AGTTATCCTG TGTCAAGGAC 
ACCATAGTCC TCCACAACGA ACTGAAGAAA CATGTGGATG GTGGCTTGGG TCCTTATGGT 
CGCACAATGT GTGATCGTAT CATCAGAGCC TGCAACCAGC GGCTGGGCAA CGGCGCTGTG 
GATGCTGGCC TTCAGGAGCG GATAGTGGAG CTGGTGGAAC TGGCTGTCCA AGGGTTTCAA 
CTGCTTGAAG AGTCCGGCAT GCAGAGCACC CCCTTTTATC TGGAGAAAAT AGTCTTTCAC 
ATCCTACAGA AAGTCGCAAG CCTTGGAGCT CATGGTCCTG CTTGTCGACT GGGTCAGCTC 
GTGTATAGAA GACTGAAATG TTTGTCTGCA GAGATTGATG ACTTTAATGT CCTGGTGCGG 
AATTGCTTTG CAGTCTTGTG GAACAGTCTC CTCTCTAACA AACCGAGTGC TTCCACCCTC 
CCTCCACGAG ACAGACTCCA CTGTCAGCTG CAAGCTTTAA CATTCAGACT CCTTGAACAG 
GGCTCCAGCT CGTCCTCTTC CCCATCCAAA GTTCCTCTGT TCGTGGAGGA AGCAGTAGTG 
GAGTACGAAC GATCATGTGG AGGTTTTACT CAAGACGATG TGAGTTTTCT GACCTCTGAA 
TTGCGTGAGC TGTTCTCTGG TGCTTTCATC AATCCTCAGG ACTCTGCATC AACTTCTTCA 
CTGCTGGCTG TGTGGTGTGA GGTGGCATTT AAAGTGTCTA AACTGCTCTG CAAAAGTAGC 
TTTTCACCTG AGGCTGTTGA ACTCTTGCGA GGCACTCTGA AGGAGGTTAA TGGCCACGTT 
GGCCTGCGTT CGGCTCTGGG TCTGGCAGAC CGGGCAGTGC AGATGCAGTG TGTGTTAAGT 
TCTGGAGGTG AATGCAGTAA GAGCTTCACT GAATGTGCCC GTGCTCTCCG GATGTTGCCT 
TGTGCCATGT CCAGCTCAGA GTCTCATGCC CTGTTAGAGG CTTGTCAGCT GGTGGTTTGG 
ACGTTGGAAG CCAGTCAGTG TAAAGGAATG GATGTGACCA CTTTGCTGGC TTGTTTTTCC 
TTTTTGGAAG AATATCAGGA ATTTCTGCTT GCACAGCAAA AGAACTCGTT TTCGCTGCAG 
GTCCAATACT CTCTTTGTTT CAGTTTTTAC CAGGGCTTCA TCTGCACATA TGATAGTCTT 
CATGCTTCTC AGGTGTCTGT GGGCGAGACC TTGGACAGAG TGCTATTGTT CTGCCGGTCG 
ACCGCAGGAC GTATGCTTGC TGAACTGCGG ACACTCAATA ATGACAACTT GCTGCTTAAA 
GCAGTGTGTG CAGTGAATAA TGTGGTGTAC GAGCTGTTTA ACAGGAAGCT GTATGAAGGA 
GCCTTTGGAT TGTCGATGAT TGTGTGTCAG GAGCTCTGTA AGGACTGCCC GCCCTCTCTT 
CCTATGGATC GGGTCAACCG CTGTTTCATG CTGACAGTGC AGTGTAGTCG CCGCGCAGGC 
CATCTGGAGC AGGCTCTGGA CTGGATTGTT CGCTGGATTC AGGCTTTGGG CTCACAGATT 
TTGGATCATC TCACTGAACC TGTGTCGATG TGGGTCAAAA CTAAGTGTGA TGCCGCCAGA 
GCAGGCGAGG ATGACACACG CCTGAGGACT CTGAGGGACG GGTTAGGAGA ATCCTTAGTG 
GATGAGGAGG TTATGATGTG TTTGTTAGAG GAGGAGTTGC GTGTATATAA GGAGCAGACT 
GGAGACACAG CACAGGAACG CTACAACACA CTCTGCGACC TCTTGGAAAT CTGCCATGAA 
GAAACCCCAC ACACACTAAG AAGAGCTACG TATCTGTGTG AGATGGCACA GGTTGTGTGC 
TATCAGGACT TCAGCCAGCA GACTGACTGC TCAGCAGTTG ATTTTACCCA CGAGGCTTTG 
CGGTTGCTGG AAGCAGAACC AGAGACGGCA GAGAATACTG ATAGGCTGAA GGATGAGAAA 
GCCCACGCTT CCCTGTGGCT CTATATCTGC ACTCTTGAGT CTAACCTGCA GGAGGCTGTG 
GACACAGAGA AGCGTTTGTG TGCAGTGCAG GAGGAGAATA AAAGAGATGT GAATATGGAT 
CCTGTTCCCA CAAATGATCT GGAGTACGAG GACAAACAGA AATCACAAGA GAGCCAGTTA 
GTCTACGATG GACTACGCTT CAACCTGCTG GCTCACAGCA AGCAAAGTGA ACCTCTAGAT 
AAATGTTTGT CTCTATGGCG GAGTCTCCTC AAGGGGGCTG TGCCTGCAGT TAGAGACCCT 
AAACTCACAG CTTCTTCCAT GACTTTGATG GCTGCCCTAT ACACACTTAT GGGCAAGCAC 
CTGCAAGCTC TGGAGGGATA TCAGCTTGCT GCCACCCTTT TCCATGTACT TGGTGATGGC 
CAGAGTAGTG CCAATGCCTT CTGCCACTCT ACCAGGATCT TGCTTTACCT GGGTTCACCG 
CAGCTTGCAC AGGTGGACTT AGAAAAAGCA GAAAAGAGCT TGTCATCTGT CACCATCTCT 
GAGGGCACAT CTATTATCTC CATGCTGGCC ATGTTGCAAA AAGCACAGCT ACACTATGCT 
CTAGGACAGG TGGAGAGTGG CGTGTCCTGC CTGATGGAGG TGATAAAGGA ATCTGCTCAG 
CATCATTCTA AGAGCTGGTA CCTGCTCAGG GCTCGAGCAC TGCAGACAGC CAGTGAATAC 
TTGAGTCTGG ACACACTGGC TTTGAACTCA CAGCTACGCC AGAGCATCAT GCAGCAGGGT 
CTAAAGACTC CAGACACTGC TCAATATGAA GGGTTGAAGC TGTTGTGTAG CCTGGTGATG 
ATGTTATTGG GCAACGGGTT TTACGGAGCG CCTGGACCAA ACACTGACAC TCGCTTTGTG 
GATAAAGGAG ACAGTGTTGT GTTTAAGTGG CTGCTGCTGA GTGAAGTGCT GGTGTGCTCG 
GAGAGAATGG TGATCACCAG GAGCAGCAGC GGAGCCGTTC ATGAAGCTAA AGCTCAGTGT 
CTAGAGGCTC TCAAACTGGC CACCAAACTA CAGACACTCA GCCACTGTGC TGAGCTGTTG 
GTGCTGAAGG CAGAGCTGGA GCTGATGAAG GGTGCAACTG AAGCTAGTAG TTTAGACCTA 
GAGCAAGTTA GACATTTACT TGATCTCTGC ACAGATTTTG GCCAGGGACG ACAACAGAAA 
TCTGAAGTGA AAATCAAACC ACGCAAAGGT CGTCCCGCTG CCCCTTCTTC CTCTGGTCAC 
ATCTCTGAGG AAGAGGATGA TCTGAGTGGG ATCCTCAGCT CTCGTGCCCT TCCGAAGGAG 
CCTGTGGAGA ACATGGCTAG ATTAGGAAGC CAAGGAGCTT CTCCCCCTCT AAAGCCCAAA 
TGCCAGCATG GCCTCTCCTG TCTGACCCAT GAAGAGACCT GCTCTTGTCT CTGCTGCACT 
GAGCTCAGCG TGGCTCGAGT CAGCATCCAC TGGGCACTAT TACAGGCAGA TCTCCAGACA 
GATCCCGAAC GCTCTCTCAG ACTACGCCAG TCTGCCAGGA AACGCTGCCG CTATGTTGTA 
ACTAAACTTC AGAGAAGTTT GGCAGCACTT GTACACTCCA AAAAATCACC TAATGTCTGC 
TTGTCACTGC TTCAGGCTGA ACAGGGTAAA GTGCATCTGG GTGCTGTGCT TCAACTGCTC 
AGAACTGGAG ACAAAGGGAA AGCTGCCATC TTGTGGGAGG AGATAGAAGC AGGATTAGAG 
GCAGTGACAC CTAAAGGAGC ACTGACACCA GAACTTGGGC CTATCAGAGC TACTCTACTG 
GGGGCTAAAG CCGTAGCCTG CTGCTTGGCG TTGGCAATGA AGAAACAGTG TATACCCGAA 
GAGCTGTTTT CTTCATTGTG GCACTGGAAT CCACCAAAGG TTAAACCGCA ATTAAAATCT 
AAGCATCGAT CCAAGAGTCC GGACGCAGCT CCTCTAGAGG CAGGTGTTAA ACATAACCCT 
AAAGTTAAGA GTGGACTTGA GGAGAATGAA AGCAGAAAAA CCAAAGAGAC CACTGTCATT 
TCGAAGAAAG CTAAAGACTC TGTGCCCAAA ATTACCATCA CAAAATCCTC CATGGTTTTT 
AAAACGCCCA AAGCAACGAG AACAACCCGG CCCAAATCTG TCTCCACTAG CACTGGCATC 
AGTGACCTGA GAGCATTTGA CTTCAATAAT GAGGTGCCTG ACATTTCAGT CAACTTCACA 
CCATCATTAC ACCGATCTAC AAACCAAAGT GCGACTGCAA AGTCAAAAGC GTCCTCAAAG 
GGTTCGTTCC AGGTTTACAA AGATTCTTCT CCTGCTGCAG AGAAAGCTGT AGTTGTCCCG 
GCTGCTCCTA AACGAACAAA GCGTTCTCGC TTCAAGGTTG AGTTCAGCGA TGAAAGCGAC 
AACGATGCTG CTCCTCTTGC TGTAATGGAA AAGTCAGAGA AAAAGAAAAA CACCTTTAAA 
ACATCACGCA CCTTGAAACC AACCCTTAAC ACCATCCCTA GTGACACCAA ACTTGTGAGC 
TCCACAGTAG ATCCAGAACC CTCCAGACGA ACACGGACCA CTAAGAAAAG CACAGCTCTT 
CCCTCCATTG GTGGCCTCTC TTCTGAGGAA GAACTTGGAG TATCTTCACA GACACGTACG 
CGTAGAGGAC GACCAAAGAA GAGCACCAGC TCAGCAGACA CAGAGGAGCC AGAGAGGATG 
AGGATGATTA AGGAGGAAGA ACAGAGGGTT CTTTTGAATG TCAGCATGGA GGAGCTCAGA 
GGCTCTGACA CTGAGACAAA AGACACTGGA AGTCCAGATG CTGACTGTGA GGTCTTGAGG 
AGAGATCTAG GTGCAGATTT TGGGCGAGAG TGTTTCAGTG AGCTCAGGAG AGCTGGACAG 
AATGGATCAG GATCTCACAC AGCTCTGCCA CATGCAACTA CATCTCCTGA CTTGTCAGTA 
GAGACGGTCC AGTCATACCT GCGCTCCTCA TGGCTCCTCC TGCACCACTT CCCGTTTCCT 
TCTCTTTATC CTCACATCTG CTCATTACTC GCTCAGTCAT TCGGTCAAAC GGACCCCATC 
ATCACAGCAA TGCTGCATGC ACAGGCACTG GGAGTGTCCA CTCGCCACCA TATGACCCGT 
CACGTGGTTA ACCAGTTCAG AAAGTTGAAG AAATCCTGTA ACGATGTAGC AGAAGGTCTT 
GGAGCTCTGA GTCTGGAGGA AACATCTGGA GCAACGCAGT CAAAGAAACT CTCTGCGTTG 
GAGCAGATCT TTTCATTCAC AGCATCAAAC CCAATGCAGT TTCCACAGAC ACACTGTCAG 
CAGTTCACAC AGCAGCTGGA AGATCTACCT GCAGGAGTGA CCGTATGCTT GCTGTCACTG 
ACGGGGGTGC ATCCTGATGA AATTGGCAAC ACTATTCTTC TGACCCGACT GGAGCGCGGC 
TCTACCCCAA TCACAGTTCG AATCCCCACT GCAGACAGAG AGCAATCTGT TGCTGGCCTG 
TTGGAAGAAA TGGATGGAGT CTTGAAAGGA CAAAAGGAAG TGAGCACAGT GGCTGAGAAA 
TCACAGTGGT GGGACGGAAG GAAGGCTTTA GATGCCCGTG TGGAGAAAAT GCTGGAGGAG 
ATGGAAGAGG CTTTAGGAGT GTGGCGAACT CTTTTTCTGC CTTTGACCTC TGATCCTAAG 
CTTGATGTTC AGGTAAAGAG CCTCCAAAAG GCTCTTAAAT CAACAAAGAT CACACGGGAC 
ACTCTCAAGG TGATTCTATC AGCGTCTCCA CTGCTGTCCC TGCCAGACCT GCAGTGTCTT 
CTGGAAGGAA TGAATGTGCA GGACAAGGAC CTGCTGAAGC TCCTGCAGAG AGGCGTGGCT 
GAACTGAGAG GGAGGGAGGA GCCACAGGGA CACACTGTTC TGATTTTAGA CAAGTACTTG 
CAGAGGTTGC CGTGGGAGAA CATTGCTTGT CTGAAGTCTC AGTCGGTCAC TCGAATGCCC 
TCATTACACG CGGTGCTTGG ACACAGTCAT CTGAGAGAGG TGGATACAAG TTGTGTTCTA 
TCTTGTGGTG TGAACCCTAA GCAGGTTTAC TATGTGCTAA ACCCAGATGG AAACCTGCCC 
GACACTGAGA ATCGCTTCAA AGAGTGGTTT ACAGGTGAAC GAACATGGCA GGGGGTTTGT 
GGAAGGGCTC CTGATCCGGA GAAACTACAA GAGGCTGTGA CCACTAAAGA TCTCTACATA 
TACATCGGAC ATGGTGCAGG AGCCCGCTTT CTGGATGCGC AGAGAGTTTT GAAGGTGCCT 
GTGAGGACCG CAGCTCTTCT GTTCGGCTGC AGTAGTGCTG CTCTCAGTGT GCTTGGTCAG 
CAGGAGGGAA CGGGCATCAT CCTCAGCTAC CTGACCGCTG GATGTCCATT GGTGCTGGGT 
AACCTGTGGG ATGTGACAGA TCGTGACTTG GATCGATTCA CATCGGCTCT GCTCCAGTCT 
TGGCTCTCTG CTGGTTCTGG CTCTTCACTT CTTCAGCATT TGGCGAAATC ACGCAGCGCA 
ACTCATTTAA AACACATAAT CGGTGCTGCG CCCATTGCGT ATGGTTTACC GGTGCATATA 
CACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	espl1
Entrez Gene ID	797406
Full Name	extra spindle pole bodies like 1, separase
Synonyms	cds,espl1,,mp:zf637-3-001691,wu:fl55e11
General protein information	Preferred Names extra spindle pole bodies like 1, separase Names separin cease and desist extra spindle pole bodies homolog 1 extra spindle poles like 1 fl55e11 separase
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

Homo sapiens (human)	ESPL1	NP_036423.4
	ESPL1	XP_001103494.1
Danio rerio (zebrafish)	espl1	XP_001337869.1
Pan troglodytes (chimpanzee)	ESPL1	XP_003313754.1
Canis lupus familiaris (dog)	ESPL1	XP_849828.2
Bos taurus (cattle)	ESPL1	NP_001039414.2
Gallus gallus (chicken)	ESPL1	XP_423289.4
Mus musculus (house mouse)	Espl1	NP_001014976.1
Rattus norvegicus (Norway rat)	Espl1	NP_001164073.1
Xenopus tropicalis (tropical clawed frog)	espl1	XP_004912005.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

espl1 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

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