cachd1 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	cachd1
Entrez Gene ID	794807
Full Name	cache domain containing 1
General protein information	Preferred Names cache domain containing 1 Names VWFA and cache domain-containing protein 1
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021471791.1	XP_021327466.1	VWFA and cache domain-containing protein 1 isoform X2
XM_021471790.1	XP_021327465.1	VWFA and cache domain-containing protein 1 isoform X2
XM_001334793.6	XP_001334829.3	VWFA and cache domain-containing protein 1
XM_001334793.7	XP_001334829.3	VWFA and cache domain-containing protein 1 isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	CACHD1	NP_065976.2
Rattus norvegicus (Norway rat)	Cachd1	NP_001178687.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009043	XP_319795.4
Canis lupus familiaris (dog)	CACHD1	XP_536680.3
Gallus gallus (chicken)	CACHD1	XP_422524.4
Danio rerio (zebrafish)	cachd1	XP_001334829.3
Drosophila melanogaster (fruit fly)	CG16868	NP_611469.1
	CACHD1	XP_001089910.2
Mus musculus (house mouse)	Cachd1	NP_932154.1
Pan troglodytes (chimpanzee)	CACHD1	XP_003308221.2
Bos taurus (cattle)	CACHD1	XP_002686374.2
Xenopus tropicalis (tropical clawed frog)	cachd1	XP_002931660.2

Gene Ontology

The following cachd1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the cachd1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa144718	XM_021471790.1 Latest version!	Danio rerio cache domain containing 1 (cachd1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
ODa144718	XM_021471791.1 Latest version!	Danio rerio cache domain containing 1 (cachd1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
ODa11929	XM_001334793.7 Latest version!	Danio rerio cache domain containing 1 (cachd1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
View Old Accession Versions >>
ODa11929	XM_001334793.6	Danio rerio cache domain containing 1 (cachd1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

cachd1 ( XM_021471791.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021327466.1 Danio rerio cache domain containing 1 (cachd1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa144718
Clone ID Related Accession (Same CDS sequence)	XM_021471791.1 , XM_021471790.1
Accession Version	XM_021471791.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3432bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	VWFA and cache domain-containing protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394651.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGAAT TTGACGGTAA TTTTAATGCC AATGTTTCCA AGACCGTCAG CTGTGATCGT 
CTCTCCCCTA CCGTCAATAG CAGAGCTTTC AACCCAGGAC GAGACCTCAA CTCTGTGCTG 
GCAGACAACC TGAAGTCCAA TCCAGGGATC AAGTGGCAGT ACTTCAGCTC AGAGGAGGGC 
ATTTTCACTC TGTTTCCTGC TCACAAGTTC CACTGCAAGG GCACTTTTGA GCACAGGAGC 
AGGCCTGTGT ATGTCGCGGC AGTAAGGCCA CAATCCAAGC ATATCGTGGT GATTATAGAC 
CATGGTGCAT CTGTCACCGA AACCCAGCTC CAGATTGCTC GAGATGCTGC TCTGGTCATC 
CTCAACTCCA TCGACGAACA TGATAAAATC TCAGTGTTGA CGATAGCAGA TACTGTTCGC 
TCCTGCTCTC TGGATCAGTG CTATAAGAGC TTCCTCTCAC CTGCTACCAG CGAGACTAAG 
AGGAAGATGA GCACCTTCAT CAGCAACATT AAAGCTTCTG ACAGCTCTAC CCAGCATGCA 
GTGGGCTTCC AGAAGGCCTT TCAGTTGTTG AGGAATACAA GCAACGTCAC CAGACAGCTG 
ACTAACACTG ACATGATGAT AATCTACCTG TCGTCGGGCA TCACCTCCCG AGACTCATCT 
GAGCATGAAA AGAGAGCAAC TCTGAGCGCA GTGAAAGAGG AGAACCGGCA CCTCAACAAC 
TCCGTCATGA TCCTCACCTA CGCTCTCATG AACGATGGAG TGACTGGACT GAAGGAGCTG 
GCATTCTTGC GAGACCTGGC TGAGCAGAAT TCCCTGAAGT ACGGCGTTCA GGATCGATCT 
GCACTGCCCG TGGTAAAGGG AAGTATGATG GTTCTGAACC AGCTCAGTAA TCTGGAGACC 
ACAGTGGGCC GCTTCTACAT CAACCTCCCC AACCGAATGA TCGACGTGGC CACCTTCAGC 
TTGCCTTATG CGGACCAGAT GGGAGACGGT TTTATCATGA CCGTCAGCCG GCCGTGCTAC 
TTTGGTAACT TGTTACTGGG TGTGGTTGGA GTGGATGTGA ACCTGGCATA CATACTGGAG 
GACGTTACCT ACTACCAGGA CTCCCTGGCC TCATATACAT TCCTCATAGA CAACAAAGGC 
TACACTCTCA TGCATCCATC ACTCACCCGT CCTTACCTGA TGACTGAAGC GCCTCTTCAT 
ACAGACATCA TCCACTATGA GAACATCCCA GGGTTTCCTG TTGTGCGTCA GAACATCCTC 
AGTCTGCCTC TGGGGAGTCA GGTCATTGCT GTGCCTGTCA ACTCTTCTCT GTCTTGGCAC 
ATGAACCGCC TCCGTGATCC TAATCGAGAT GCCTACAATG TTAGCTATGC TTGGAAACTG 
GTTCAGGACA CTTCGTTTAT CCTCTGCATA GTTTATGTTC AGCCAGAGAT CCCGGTGAAG 
CAGCTGAAAA ATCTGAACAC AGCCCCCAGC AGCAAACTTC TCTACCACCG CCTGGATCTG 
CTGGGGCAGC CCAGCTCCTG CCTTCACTTT AAACAGCTCG CTACAGTCGA GAGCCCTACG 
GTGATGTTGT CCGCTGGTAG TTTCTCCTCT CCCTATGAGC ACCTGAGTCA ACCAGAGACC 
AAGCGTATGG TGGAGCACTA CACTGCTTAC CTGAGCGACA ACACTCGACT TATAGCCAAC 
CCCGGCCTGA AGTCTTCTGT GAGGAATGAG GTGATGGCCA CCAGCCACGT GACTGATGAA 
TGGATGAGTC TTATGGAGAT GAGCAGCCTC AACTGCTACA TTGTGAGGCG TTACATAGCA 
ACGCCCAATG GGGTGCTGCG GATTTACCCC GGTTCACTCA TGGACAAAGC CTTCGATCCC 
ACCAGGAGGC AATGGTACCT ACATGCTGTA GCCAATCCTG GTCTCATCAC TTTTACTGGA 
CCGTACCTGG ATGTTGGTGG GGCAGGTTAT GTAGTCACCA TTAGCCACAC CATCCACGCC 
CCTAGTTCCC AAATGGCCTC TGGTTACACA GTAGCGGTAA TGGGCATTGA CTTCACCCTG 
CGCTACTTCT ACAAAGTTCT GTTGGATCTT CTTCCCATCT GCAACCAGGA CAAAGGCAAC 
AAAATTCGGT GTTTTATCAT GGAGGACAGA GGCTATTTGG TGGCCCATCC GACACTGATT 
GATCCGAAGG GTCACGCTCC TGCAGAACAA CAGCATATTA CGCATAAGGA GCCTCTAGTG 
GCCAATGATA TTCTCAACCA CCCCAATTTT GTGAAGAAAA ACCTGTGTAA CAGTTTCAGC 
GATCGCACTG TGCAGCGTTT CTACAAGTTC AACACAAGCA TCGTGGGTGA TCTGACAAAC 
CTTGTGCATG GCAGTCACTG CTCAAAGTAC CGGCTTACTC GTATCCCAGG AACGAATGCG 
TTTGCTGGTA TTGTGAATGA GACGTGTGAT GCGCTGGCCT TCTGTGCTTG CAGTACTGTG 
GACCGTCTCT GCCTCAACTG CCACAGAATG GAGCAGAATG AATGTGAGTG CCCCTGTGAA 
TGCCCTCTAG AGGTGAACGA GTGTACTGGC AACCTCACCA ACGCTGAGAA CAGAAATCCG 
AGCTGTGACG TGCACCAGGA GCCTTTGTCT TTGACTGTAA TTGAGCCCAG TCTACAGGAT 
ACTCTGCCAC AGTGCATCAA CACCAGATGC AGCCAGCGCT TTACCAGCAG TGACTGTTTT 
GGTGTTCTGG ACTGTGAGTG GTGTATGGTG GACAGCGATG GAAAGACTCA CCTGGATAAG 
CCTTACTGTG CCCTGCAGAA GGAGTGTTTC GGAGGCATAG TGGGCGCCAA GAGTCCTTAT 
GTGGATGGAA TGGGCATTTT AGATGAGGAG TTGGCTTCGC TGAACATGAT CAAGAGTGCT 
CCTGTGGGCC CTGTGGCTGG AGGGATTATG GGATGCATCA TGGTTTTGGT GTTGGCAGTT 
TATGCCTATA GGCACCAGAT CCACCGTCGC AGTCATCAGC ACATGTCACC GCTGGCCGCA 
CAGGAAATGT CTGTGCGGAT GTCCAACCTG GACAACGAGC GAGATGACAG AGATGAAGAC 
AGCCACGAAG ACAGAGGAAT AATCAGCAAC ACTCGATTCA TTGCTGCAGT TATTGAGAGG 
CACACACACA GTCCAGAACG ACGACGGAGA TACTGGGGCC GGTCAGGGAC TGAAAGTGAC 
CACGGTTACA GTACCATGAG CCCTCAGGAG GACAGTGAGA ACCCGCCCTG CAATAACGAT 
CCGCTCTCAG CGGGCGTTGA TGTGGGCAAT CACGACGAGG ACCTTGACCT GGACACTCCT 
CCACAGACAG CTGCTCTTCT CAGCCACAAA TTCCACCCCT ATCGGCACCC CCACACACAC 
CACCCCCTGC ATCTGCACAC ACACCACCTT CAGGCCGCCG TCACCGTTCA CAGTGTGGAC 
GCTGAGTGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021327466.1
CDS	237..3668
Translation	MMEFDGNFNANVSKTVSCDRLSPTVNSRAFNPGRDLNSVLADNLKSNPGIKWQYFSSEEGIFTLFPAHKFHCKGTFEHRSRPVYVAAVRPQSKHIVVIIDHGASVTETQLQIARDAALVILNSIDEHDKISVLTIADTVRSCSLDQCYKSFLSPATSETKRKMSTFISNIKASDSSTQHAVGFQKAFQLLRNTSNVTRQLTNTDMMIIYLSSGITSRDSSEHEKRATLSAVKEENRHLNNSVMILTYALMNDGVTGLKELAFLRDLAEQNSLKYGVQDRSALPVVKGSMMVLNQLSNLETTVGRFYINLPNRMIDVATFSLPYADQMGDGFIMTVSRPCYFGNLLLGVVGVDVNLAYILEDVTYYQDSLASYTFLIDNKGYTLMHPSLTRPYLMTEAPLHTDIIHYENIPGFPVVRQNILSLPLGSQVIAVPVNSSLSWHMNRLRDPNRDAYNVSYAWKLVQDTSFILCIVYVQPEIPVKQLKNLNTAPSSKLLYHRLDLLGQPSSCLHFKQLATVESPTVMLSAGSFSSPYEHLSQPETKRMVEHYTAYLSDNTRLIANPGLKSSVRNEVMATSHVTDEWMSLMEMSSLNCYIVRRYIATPNGVLRIYPGSLMDKAFDPTRRQWYLHAVANPGLITFTGPYLDVGGAGYVVTISHTIHAPSSQMASGYTVAVMGIDFTLRYFYKVLLDLLPICNQDKGNKIRCFIMEDRGYLVAHPTLIDPKGHAPAEQQHITHKEPLVANDILNHPNFVKKNLCNSFSDRTVQRFYKFNTSIVGDLTNLVHGSHCSKYRLTRIPGTNAFAGIVNETCDALAFCACSTVDRLCLNCHRMEQNECECPCECPLEVNECTGNLTNAENRNPSCDVHQEPLSLTVIEPSLQDTLPQCINTRCSQRFTSSDCFGVLDCEWCMVDSDGKTHLDKPYCALQKECFGGIVGAKSPYVDGMGILDEELASLNMIKSAPVGPVAGGIMGCIMVLVLAVYAYRHQIHRRSHQHMSPLAAQEMSVRMSNLDNERDDRDEDSHEDRGIISNTRFIAAVIERHTHSPERRRRYWGRSGTESDHGYSTMSPQEDSENPPCNNDPLSAGVDVGNHDEDLDLDTPPQTAALLSHKFHPYRHPHTHHPLHLHTHHLQAAVTVHSVDAEC

Target ORF information:

RefSeq Version	XM_021471791.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio cache domain containing 1 (cachd1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021471791.1

ORF Insert Sequence:

ATGATGGAAT TTGACGGTAA TTTTAATGCC AATGTTTCCA AGACCGTCAG CTGTGATCGT 
CTCTCCCCTA CCGTCAATAG CAGAGCTTTC AACCCAGGAC GAGACCTCAA CTCTGTGCTG 
GCAGACAACC TGAAGTCCAA TCCAGGGATC AAGTGGCAGT ACTTCAGCTC AGAGGAGGGC 
ATTTTCACTC TGTTTCCTGC TCACAAGTTC CACTGCAAGG GCACTTTTGA GCACAGGAGC 
AGGCCTGTGT ATGTCGCGGC AGTAAGGCCA CAATCCAAGC ATATCGTGGT GATTATAGAC 
CATGGTGCAT CTGTCACCGA AACCCAGCTC CAGATTGCTC GAGATGCTGC TCTGGTCATC 
CTCAACTCCA TCGACGAACA TGATAAAATC TCAGTGTTGA CGATAGCAGA TACTGTTCGC 
TCCTGCTCTC TGGATCAGTG CTATAAGAGC TTCCTCTCAC CTGCTACCAG CGAGACTAAG 
AGGAAGATGA GCACCTTCAT CAGCAACATT AAAGCTTCTG ACAGCTCTAC CCAGCATGCA 
GTGGGCTTCC AGAAGGCCTT TCAGTTGTTG AGGAATACAA GCAACGTCAC CAGACAGCTG 
ACTAACACTG ACATGATGAT AATCTACCTG TCGTCGGGCA TCACCTCCCG AGACTCATCT 
GAGCATGAAA AGAGAGCAAC TCTGAGCGCA GTGAAAGAGG AGAACCGGCA CCTCAACAAC 
TCCGTCATGA TCCTCACCTA CGCTCTCATG AACGATGGAG TGACTGGACT GAAGGAGCTG 
GCATTCTTGC GAGACCTGGC TGAGCAGAAT TCCCTGAAGT ACGGCGTTCA GGATCGATCT 
GCACTGCCCG TGGTAAAGGG AAGTATGATG GTTCTGAACC AGCTCAGTAA TCTGGAGACC 
ACAGTGGGCC GCTTCTACAT CAACCTCCCC AACCGAATGA TCGACGTGGC CACCTTCAGC 
TTGCCTTATG CGGACCAGAT GGGAGACGGT TTTATCATGA CCGTCAGCCG GCCGTGCTAC 
TTTGGTAACT TGTTACTGGG TGTGGTTGGA GTGGATGTGA ACCTGGCATA CATACTGGAG 
GACGTTACCT ACTACCAGGA CTCCCTGGCC TCATATACAT TCCTCATAGA CAACAAAGGC 
TACACTCTCA TGCATCCATC ACTCACCCGT CCTTACCTGA TGACTGAAGC GCCTCTTCAT 
ACAGACATCA TCCACTATGA GAACATCCCA GGGTTTCCTG TTGTGCGTCA GAACATCCTC 
AGTCTGCCTC TGGGGAGTCA GGTCATTGCT GTGCCTGTCA ACTCTTCTCT GTCTTGGCAC 
ATGAACCGCC TCCGTGATCC TAATCGAGAT GCCTACAATG TTAGCTATGC TTGGAAACTG 
GTTCAGGACA CTTCGTTTAT CCTCTGCATA GTTTATGTTC AGCCAGAGAT CCCGGTGAAG 
CAGCTGAAAA ATCTGAACAC AGCCCCCAGC AGCAAACTTC TCTACCACCG CCTGGATCTG 
CTGGGGCAGC CCAGCTCCTG CCTTCACTTT AAACAGCTCG CTACAGTCGA GAGCCCTACG 
GTGATGTTGT CCGCTGGTAG TTTCTCCTCT CCCTATGAGC ACCTGAGTCA ACCAGAGACC 
AAGCGTATGG TGGAGCACTA CACTGCTTAC CTGAGCGACA ACACTCGACT TATAGCCAAC 
CCCGGCCTGA AGTCTTCTGT GAGGAATGAG GTGATGGCCA CCAGCCACGT GACTGATGAA 
TGGATGAGTC TTATGGAGAT GAGCAGCCTC AACTGCTACA TTGTGAGGCG TTACATAGCA 
ACGCCCAATG GGGTGCTGCG GATTTACCCC GGTTCACTCA TGGACAAAGC CTTCGATCCC 
ACCAGGAGGC AATGGTACCT ACATGCTGTA GCCAATCCTG GTCTCATCAC TTTTACTGGA 
CCGTACCTGG ATGTTGGTGG GGCAGGTTAT GTAGTCACCA TTAGCCACAC CATCCACGCC 
CCTAGTTCCC AAATGGCCTC TGGTTACACA GTAGCGGTAA TGGGCATTGA CTTCACCCTG 
CGCTACTTCT ACAAAGTTCT GTTGGATCTT CTTCCCATCT GCAACCAGGA CAAAGGCAAC 
AAAATTCGGT GTTTTATCAT GGAGGACAGA GGCTATTTGG TGGCCCATCC GACACTGATT 
GATCCGAAGG GTCACGCTCC TGCAGAACAA CAGCATATTA CGCATAAGGA GCCTCTAGTG 
GCCAATGATA TTCTCAACCA CCCCAATTTT GTGAAGAAAA ACCTGTGTAA CAGTTTCAGC 
GATCGCACTG TGCAGCGTTT CTACAAGTTC AACACAAGCA TCGTGGGTGA TCTGACAAAC 
CTTGTGCATG GCAGTCACTG CTCAAAGTAC CGGCTTACTC GTATCCCAGG AACGAATGCG 
TTTGCTGGTA TTGTGAATGA GACGTGTGAT GCGCTGGCCT TCTGTGCTTG CAGTACTGTG 
GACCGTCTCT GCCTCAACTG CCACAGAATG GAGCAGAATG AATGTGAGTG CCCCTGTGAA 
TGCCCTCTAG AGGTGAACGA GTGTACTGGC AACCTCACCA ACGCTGAGAA CAGAAATCCG 
AGCTGTGACG TGCACCAGGA GCCTTTGTCT TTGACTGTAA TTGAGCCCAG TCTACAGGAT 
ACTCTGCCAC AGTGCATCAA CACCAGATGC AGCCAGCGCT TTACCAGCAG TGACTGTTTT 
GGTGTTCTGG ACTGTGAGTG GTGTATGGTG GACAGCGATG GAAAGACTCA CCTGGATAAG 
CCTTACTGTG CCCTGCAGAA GGAGTGTTTC GGAGGCATAG TGGGCGCCAA GAGTCCTTAT 
GTGGATGGAA TGGGCATTTT AGATGAGGAG TTGGCTTCGC TGAACATGAT CAAGAGTGCT 
CCTGTGGGCC CTGTGGCTGG AGGGATTATG GGATGCATCA TGGTTTTGGT GTTGGCAGTT 
TATGCCTATA GGCACCAGAT CCACCGTCGC AGTCATCAGC ACATGTCACC GCTGGCCGCA 
CAGGAAATGT CTGTGCGGAT GTCCAACCTG GACAACGAGC GAGATGACAG AGATGAAGAC 
AGCCACGAAG ACAGAGGAAT AATCAGCAAC ACTCGATTCA TTGCTGCAGT TATTGAGAGG 
CACACACACA GTCCAGAACG ACGACGGAGA TACTGGGGCC GGTCAGGGAC TGAAAGTGAC 
CACGGTTACA GTACCATGAG CCCTCAGGAG GACAGTGAGA ACCCGCCCTG CAATAACGAT 
CCGCTCTCAG CGGGCGTTGA TGTGGGCAAT CACGACGAGG ACCTTGACCT GGACACTCCT 
CCACAGACAG CTGCTCTTCT CAGCCACAAA TTCCACCCCT ATCGGCACCC CCACACACAC 
CACCCCCTGC ATCTGCACAC ACACCACCTT CAGGCCGCCG TCACCGTTCA CAGTGTGGAC 
GCTGAGTGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cachd1 ( XM_021471790.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021327465.1 Danio rerio cache domain containing 1 (cachd1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa144718
Clone ID Related Accession (Same CDS sequence)	XM_021471791.1 , XM_021471790.1
Accession Version	XM_021471790.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3432bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	VWFA and cache domain-containing protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394651.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGAAT TTGACGGTAA TTTTAATGCC AATGTTTCCA AGACCGTCAG CTGTGATCGT 
CTCTCCCCTA CCGTCAATAG CAGAGCTTTC AACCCAGGAC GAGACCTCAA CTCTGTGCTG 
GCAGACAACC TGAAGTCCAA TCCAGGGATC AAGTGGCAGT ACTTCAGCTC AGAGGAGGGC 
ATTTTCACTC TGTTTCCTGC TCACAAGTTC CACTGCAAGG GCACTTTTGA GCACAGGAGC 
AGGCCTGTGT ATGTCGCGGC AGTAAGGCCA CAATCCAAGC ATATCGTGGT GATTATAGAC 
CATGGTGCAT CTGTCACCGA AACCCAGCTC CAGATTGCTC GAGATGCTGC TCTGGTCATC 
CTCAACTCCA TCGACGAACA TGATAAAATC TCAGTGTTGA CGATAGCAGA TACTGTTCGC 
TCCTGCTCTC TGGATCAGTG CTATAAGAGC TTCCTCTCAC CTGCTACCAG CGAGACTAAG 
AGGAAGATGA GCACCTTCAT CAGCAACATT AAAGCTTCTG ACAGCTCTAC CCAGCATGCA 
GTGGGCTTCC AGAAGGCCTT TCAGTTGTTG AGGAATACAA GCAACGTCAC CAGACAGCTG 
ACTAACACTG ACATGATGAT AATCTACCTG TCGTCGGGCA TCACCTCCCG AGACTCATCT 
GAGCATGAAA AGAGAGCAAC TCTGAGCGCA GTGAAAGAGG AGAACCGGCA CCTCAACAAC 
TCCGTCATGA TCCTCACCTA CGCTCTCATG AACGATGGAG TGACTGGACT GAAGGAGCTG 
GCATTCTTGC GAGACCTGGC TGAGCAGAAT TCCCTGAAGT ACGGCGTTCA GGATCGATCT 
GCACTGCCCG TGGTAAAGGG AAGTATGATG GTTCTGAACC AGCTCAGTAA TCTGGAGACC 
ACAGTGGGCC GCTTCTACAT CAACCTCCCC AACCGAATGA TCGACGTGGC CACCTTCAGC 
TTGCCTTATG CGGACCAGAT GGGAGACGGT TTTATCATGA CCGTCAGCCG GCCGTGCTAC 
TTTGGTAACT TGTTACTGGG TGTGGTTGGA GTGGATGTGA ACCTGGCATA CATACTGGAG 
GACGTTACCT ACTACCAGGA CTCCCTGGCC TCATATACAT TCCTCATAGA CAACAAAGGC 
TACACTCTCA TGCATCCATC ACTCACCCGT CCTTACCTGA TGACTGAAGC GCCTCTTCAT 
ACAGACATCA TCCACTATGA GAACATCCCA GGGTTTCCTG TTGTGCGTCA GAACATCCTC 
AGTCTGCCTC TGGGGAGTCA GGTCATTGCT GTGCCTGTCA ACTCTTCTCT GTCTTGGCAC 
ATGAACCGCC TCCGTGATCC TAATCGAGAT GCCTACAATG TTAGCTATGC TTGGAAACTG 
GTTCAGGACA CTTCGTTTAT CCTCTGCATA GTTTATGTTC AGCCAGAGAT CCCGGTGAAG 
CAGCTGAAAA ATCTGAACAC AGCCCCCAGC AGCAAACTTC TCTACCACCG CCTGGATCTG 
CTGGGGCAGC CCAGCTCCTG CCTTCACTTT AAACAGCTCG CTACAGTCGA GAGCCCTACG 
GTGATGTTGT CCGCTGGTAG TTTCTCCTCT CCCTATGAGC ACCTGAGTCA ACCAGAGACC 
AAGCGTATGG TGGAGCACTA CACTGCTTAC CTGAGCGACA ACACTCGACT TATAGCCAAC 
CCCGGCCTGA AGTCTTCTGT GAGGAATGAG GTGATGGCCA CCAGCCACGT GACTGATGAA 
TGGATGAGTC TTATGGAGAT GAGCAGCCTC AACTGCTACA TTGTGAGGCG TTACATAGCA 
ACGCCCAATG GGGTGCTGCG GATTTACCCC GGTTCACTCA TGGACAAAGC CTTCGATCCC 
ACCAGGAGGC AATGGTACCT ACATGCTGTA GCCAATCCTG GTCTCATCAC TTTTACTGGA 
CCGTACCTGG ATGTTGGTGG GGCAGGTTAT GTAGTCACCA TTAGCCACAC CATCCACGCC 
CCTAGTTCCC AAATGGCCTC TGGTTACACA GTAGCGGTAA TGGGCATTGA CTTCACCCTG 
CGCTACTTCT ACAAAGTTCT GTTGGATCTT CTTCCCATCT GCAACCAGGA CAAAGGCAAC 
AAAATTCGGT GTTTTATCAT GGAGGACAGA GGCTATTTGG TGGCCCATCC GACACTGATT 
GATCCGAAGG GTCACGCTCC TGCAGAACAA CAGCATATTA CGCATAAGGA GCCTCTAGTG 
GCCAATGATA TTCTCAACCA CCCCAATTTT GTGAAGAAAA ACCTGTGTAA CAGTTTCAGC 
GATCGCACTG TGCAGCGTTT CTACAAGTTC AACACAAGCA TCGTGGGTGA TCTGACAAAC 
CTTGTGCATG GCAGTCACTG CTCAAAGTAC CGGCTTACTC GTATCCCAGG AACGAATGCG 
TTTGCTGGTA TTGTGAATGA GACGTGTGAT GCGCTGGCCT TCTGTGCTTG CAGTACTGTG 
GACCGTCTCT GCCTCAACTG CCACAGAATG GAGCAGAATG AATGTGAGTG CCCCTGTGAA 
TGCCCTCTAG AGGTGAACGA GTGTACTGGC AACCTCACCA ACGCTGAGAA CAGAAATCCG 
AGCTGTGACG TGCACCAGGA GCCTTTGTCT TTGACTGTAA TTGAGCCCAG TCTACAGGAT 
ACTCTGCCAC AGTGCATCAA CACCAGATGC AGCCAGCGCT TTACCAGCAG TGACTGTTTT 
GGTGTTCTGG ACTGTGAGTG GTGTATGGTG GACAGCGATG GAAAGACTCA CCTGGATAAG 
CCTTACTGTG CCCTGCAGAA GGAGTGTTTC GGAGGCATAG TGGGCGCCAA GAGTCCTTAT 
GTGGATGGAA TGGGCATTTT AGATGAGGAG TTGGCTTCGC TGAACATGAT CAAGAGTGCT 
CCTGTGGGCC CTGTGGCTGG AGGGATTATG GGATGCATCA TGGTTTTGGT GTTGGCAGTT 
TATGCCTATA GGCACCAGAT CCACCGTCGC AGTCATCAGC ACATGTCACC GCTGGCCGCA 
CAGGAAATGT CTGTGCGGAT GTCCAACCTG GACAACGAGC GAGATGACAG AGATGAAGAC 
AGCCACGAAG ACAGAGGAAT AATCAGCAAC ACTCGATTCA TTGCTGCAGT TATTGAGAGG 
CACACACACA GTCCAGAACG ACGACGGAGA TACTGGGGCC GGTCAGGGAC TGAAAGTGAC 
CACGGTTACA GTACCATGAG CCCTCAGGAG GACAGTGAGA ACCCGCCCTG CAATAACGAT 
CCGCTCTCAG CGGGCGTTGA TGTGGGCAAT CACGACGAGG ACCTTGACCT GGACACTCCT 
CCACAGACAG CTGCTCTTCT CAGCCACAAA TTCCACCCCT ATCGGCACCC CCACACACAC 
CACCCCCTGC ATCTGCACAC ACACCACCTT CAGGCCGCCG TCACCGTTCA CAGTGTGGAC 
GCTGAGTGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021327465.1
CDS	121..3552
Translation	MMEFDGNFNANVSKTVSCDRLSPTVNSRAFNPGRDLNSVLADNLKSNPGIKWQYFSSEEGIFTLFPAHKFHCKGTFEHRSRPVYVAAVRPQSKHIVVIIDHGASVTETQLQIARDAALVILNSIDEHDKISVLTIADTVRSCSLDQCYKSFLSPATSETKRKMSTFISNIKASDSSTQHAVGFQKAFQLLRNTSNVTRQLTNTDMMIIYLSSGITSRDSSEHEKRATLSAVKEENRHLNNSVMILTYALMNDGVTGLKELAFLRDLAEQNSLKYGVQDRSALPVVKGSMMVLNQLSNLETTVGRFYINLPNRMIDVATFSLPYADQMGDGFIMTVSRPCYFGNLLLGVVGVDVNLAYILEDVTYYQDSLASYTFLIDNKGYTLMHPSLTRPYLMTEAPLHTDIIHYENIPGFPVVRQNILSLPLGSQVIAVPVNSSLSWHMNRLRDPNRDAYNVSYAWKLVQDTSFILCIVYVQPEIPVKQLKNLNTAPSSKLLYHRLDLLGQPSSCLHFKQLATVESPTVMLSAGSFSSPYEHLSQPETKRMVEHYTAYLSDNTRLIANPGLKSSVRNEVMATSHVTDEWMSLMEMSSLNCYIVRRYIATPNGVLRIYPGSLMDKAFDPTRRQWYLHAVANPGLITFTGPYLDVGGAGYVVTISHTIHAPSSQMASGYTVAVMGIDFTLRYFYKVLLDLLPICNQDKGNKIRCFIMEDRGYLVAHPTLIDPKGHAPAEQQHITHKEPLVANDILNHPNFVKKNLCNSFSDRTVQRFYKFNTSIVGDLTNLVHGSHCSKYRLTRIPGTNAFAGIVNETCDALAFCACSTVDRLCLNCHRMEQNECECPCECPLEVNECTGNLTNAENRNPSCDVHQEPLSLTVIEPSLQDTLPQCINTRCSQRFTSSDCFGVLDCEWCMVDSDGKTHLDKPYCALQKECFGGIVGAKSPYVDGMGILDEELASLNMIKSAPVGPVAGGIMGCIMVLVLAVYAYRHQIHRRSHQHMSPLAAQEMSVRMSNLDNERDDRDEDSHEDRGIISNTRFIAAVIERHTHSPERRRRYWGRSGTESDHGYSTMSPQEDSENPPCNNDPLSAGVDVGNHDEDLDLDTPPQTAALLSHKFHPYRHPHTHHPLHLHTHHLQAAVTVHSVDAEC

Target ORF information:

RefSeq Version	XM_021471790.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio cache domain containing 1 (cachd1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021471790.1

ORF Insert Sequence:

ATGATGGAAT TTGACGGTAA TTTTAATGCC AATGTTTCCA AGACCGTCAG CTGTGATCGT 
CTCTCCCCTA CCGTCAATAG CAGAGCTTTC AACCCAGGAC GAGACCTCAA CTCTGTGCTG 
GCAGACAACC TGAAGTCCAA TCCAGGGATC AAGTGGCAGT ACTTCAGCTC AGAGGAGGGC 
ATTTTCACTC TGTTTCCTGC TCACAAGTTC CACTGCAAGG GCACTTTTGA GCACAGGAGC 
AGGCCTGTGT ATGTCGCGGC AGTAAGGCCA CAATCCAAGC ATATCGTGGT GATTATAGAC 
CATGGTGCAT CTGTCACCGA AACCCAGCTC CAGATTGCTC GAGATGCTGC TCTGGTCATC 
CTCAACTCCA TCGACGAACA TGATAAAATC TCAGTGTTGA CGATAGCAGA TACTGTTCGC 
TCCTGCTCTC TGGATCAGTG CTATAAGAGC TTCCTCTCAC CTGCTACCAG CGAGACTAAG 
AGGAAGATGA GCACCTTCAT CAGCAACATT AAAGCTTCTG ACAGCTCTAC CCAGCATGCA 
GTGGGCTTCC AGAAGGCCTT TCAGTTGTTG AGGAATACAA GCAACGTCAC CAGACAGCTG 
ACTAACACTG ACATGATGAT AATCTACCTG TCGTCGGGCA TCACCTCCCG AGACTCATCT 
GAGCATGAAA AGAGAGCAAC TCTGAGCGCA GTGAAAGAGG AGAACCGGCA CCTCAACAAC 
TCCGTCATGA TCCTCACCTA CGCTCTCATG AACGATGGAG TGACTGGACT GAAGGAGCTG 
GCATTCTTGC GAGACCTGGC TGAGCAGAAT TCCCTGAAGT ACGGCGTTCA GGATCGATCT 
GCACTGCCCG TGGTAAAGGG AAGTATGATG GTTCTGAACC AGCTCAGTAA TCTGGAGACC 
ACAGTGGGCC GCTTCTACAT CAACCTCCCC AACCGAATGA TCGACGTGGC CACCTTCAGC 
TTGCCTTATG CGGACCAGAT GGGAGACGGT TTTATCATGA CCGTCAGCCG GCCGTGCTAC 
TTTGGTAACT TGTTACTGGG TGTGGTTGGA GTGGATGTGA ACCTGGCATA CATACTGGAG 
GACGTTACCT ACTACCAGGA CTCCCTGGCC TCATATACAT TCCTCATAGA CAACAAAGGC 
TACACTCTCA TGCATCCATC ACTCACCCGT CCTTACCTGA TGACTGAAGC GCCTCTTCAT 
ACAGACATCA TCCACTATGA GAACATCCCA GGGTTTCCTG TTGTGCGTCA GAACATCCTC 
AGTCTGCCTC TGGGGAGTCA GGTCATTGCT GTGCCTGTCA ACTCTTCTCT GTCTTGGCAC 
ATGAACCGCC TCCGTGATCC TAATCGAGAT GCCTACAATG TTAGCTATGC TTGGAAACTG 
GTTCAGGACA CTTCGTTTAT CCTCTGCATA GTTTATGTTC AGCCAGAGAT CCCGGTGAAG 
CAGCTGAAAA ATCTGAACAC AGCCCCCAGC AGCAAACTTC TCTACCACCG CCTGGATCTG 
CTGGGGCAGC CCAGCTCCTG CCTTCACTTT AAACAGCTCG CTACAGTCGA GAGCCCTACG 
GTGATGTTGT CCGCTGGTAG TTTCTCCTCT CCCTATGAGC ACCTGAGTCA ACCAGAGACC 
AAGCGTATGG TGGAGCACTA CACTGCTTAC CTGAGCGACA ACACTCGACT TATAGCCAAC 
CCCGGCCTGA AGTCTTCTGT GAGGAATGAG GTGATGGCCA CCAGCCACGT GACTGATGAA 
TGGATGAGTC TTATGGAGAT GAGCAGCCTC AACTGCTACA TTGTGAGGCG TTACATAGCA 
ACGCCCAATG GGGTGCTGCG GATTTACCCC GGTTCACTCA TGGACAAAGC CTTCGATCCC 
ACCAGGAGGC AATGGTACCT ACATGCTGTA GCCAATCCTG GTCTCATCAC TTTTACTGGA 
CCGTACCTGG ATGTTGGTGG GGCAGGTTAT GTAGTCACCA TTAGCCACAC CATCCACGCC 
CCTAGTTCCC AAATGGCCTC TGGTTACACA GTAGCGGTAA TGGGCATTGA CTTCACCCTG 
CGCTACTTCT ACAAAGTTCT GTTGGATCTT CTTCCCATCT GCAACCAGGA CAAAGGCAAC 
AAAATTCGGT GTTTTATCAT GGAGGACAGA GGCTATTTGG TGGCCCATCC GACACTGATT 
GATCCGAAGG GTCACGCTCC TGCAGAACAA CAGCATATTA CGCATAAGGA GCCTCTAGTG 
GCCAATGATA TTCTCAACCA CCCCAATTTT GTGAAGAAAA ACCTGTGTAA CAGTTTCAGC 
GATCGCACTG TGCAGCGTTT CTACAAGTTC AACACAAGCA TCGTGGGTGA TCTGACAAAC 
CTTGTGCATG GCAGTCACTG CTCAAAGTAC CGGCTTACTC GTATCCCAGG AACGAATGCG 
TTTGCTGGTA TTGTGAATGA GACGTGTGAT GCGCTGGCCT TCTGTGCTTG CAGTACTGTG 
GACCGTCTCT GCCTCAACTG CCACAGAATG GAGCAGAATG AATGTGAGTG CCCCTGTGAA 
TGCCCTCTAG AGGTGAACGA GTGTACTGGC AACCTCACCA ACGCTGAGAA CAGAAATCCG 
AGCTGTGACG TGCACCAGGA GCCTTTGTCT TTGACTGTAA TTGAGCCCAG TCTACAGGAT 
ACTCTGCCAC AGTGCATCAA CACCAGATGC AGCCAGCGCT TTACCAGCAG TGACTGTTTT 
GGTGTTCTGG ACTGTGAGTG GTGTATGGTG GACAGCGATG GAAAGACTCA CCTGGATAAG 
CCTTACTGTG CCCTGCAGAA GGAGTGTTTC GGAGGCATAG TGGGCGCCAA GAGTCCTTAT 
GTGGATGGAA TGGGCATTTT AGATGAGGAG TTGGCTTCGC TGAACATGAT CAAGAGTGCT 
CCTGTGGGCC CTGTGGCTGG AGGGATTATG GGATGCATCA TGGTTTTGGT GTTGGCAGTT 
TATGCCTATA GGCACCAGAT CCACCGTCGC AGTCATCAGC ACATGTCACC GCTGGCCGCA 
CAGGAAATGT CTGTGCGGAT GTCCAACCTG GACAACGAGC GAGATGACAG AGATGAAGAC 
AGCCACGAAG ACAGAGGAAT AATCAGCAAC ACTCGATTCA TTGCTGCAGT TATTGAGAGG 
CACACACACA GTCCAGAACG ACGACGGAGA TACTGGGGCC GGTCAGGGAC TGAAAGTGAC 
CACGGTTACA GTACCATGAG CCCTCAGGAG GACAGTGAGA ACCCGCCCTG CAATAACGAT 
CCGCTCTCAG CGGGCGTTGA TGTGGGCAAT CACGACGAGG ACCTTGACCT GGACACTCCT 
CCACAGACAG CTGCTCTTCT CAGCCACAAA TTCCACCCCT ATCGGCACCC CCACACACAC 
CACCCCCTGC ATCTGCACAC ACACCACCTT CAGGCCGCCG TCACCGTTCA CAGTGTGGAC 
GCTGAGTGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cachd1 ( XM_001334793.6 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_001334829.3 Danio rerio cache domain containing 1 (cachd1), mRNA.

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CloneID	ODa11929
Clone ID Related Accession (Same CDS sequence)	XM_001334793.6 , XM_001334793.7
Accession Version	XM_001334793.6	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3873bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	VWFA and cache domain-containing protein 1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_001334793.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAGTC GGTCGCGGAC AGGACCTCGA TTCTCCTTCG CGGCGTGGAG CAAACACTCC 
CGACCAAACT GGAATAACCT GGAGATCTCA AAGTGGAGCC TAGTCGCCCT GCTGGTGTTT 
GCTTGTTGTG GATTTTGTGG CGCTGATAGT GAGTTTTCGA TCCTGGAAGA GGCTCAAGTT 
TTGGCCGTGC AGATGAAGAA GCTTTCGACT CAGGAGCTCG GTGTTCTCAC CATGCAGAGA 
ATCTTCAACT CCTTTGTTTA TACAGAGAAG ACTTCAAATG GTGAAACAGA GGTGCAGCAG 
CTAGCGAAAA AGATCCGAGA GAAGTTTAAC CGGTACCTGG ATGTGGTGAA CAGGAACAAG 
CAGGTGGTGG AAGCTTCATA CACGGCCCAC CTCACTTCAC CTCTCACAGC CATACAGGAC 
TGCTGCTCCA TCCCTCCCTC CATGATGGAA TTTGACGGTA ATTTTAATGC CAATGTTTCC 
AAGACCGTCA GCTGTGATCG TCTCTCCCCT ACCGTCAATA GCAGAGCTTT CAACCCAGGA 
CGAGACCTCA ACTCTGTGCT GGCAGACAAC CTGAAGTCCA ATCCAGGGAT CAAGTGGCAG 
TACTTCAGCT CAGAGGAGGG CATTTTCACT CTGTTTCCTG CTCACAAGTT CCACTGCAAG 
GGCACTTTTG AGCACAGGAG CAGGCCTGTG TATGTCGCGG CAGTAAGGCC ACAATCCAAG 
CATATCGTGG TGATTATAGA CCATGGTGCA TCTGTCACCG AAACCCAGCT CCAGATTGCT 
CGAGATGCTG CTCTGGTCAT CCTCAACTCC ATCGACGAAC ATGATAAAAT CTCAGTGTTG 
ACGATAGCAG ATACTGTTCG CTCCTGCTCT CTGGATCAGT GCTATAAGAG CTTCCTCTCA 
CCTGCTACCA GCGAGACTAA GAGGAAGATG AGCACCTTCA TCAGCAACAT TAAAGCTTCT 
GACAGCTCTA CCCAGCATGC AGTGGGCTTC CAGAAGGCCT TTCAGTTGTT GAGGAATACA 
AGCAACGTCA CCAGACAGCT GACTAACACT GACATGATGA TAATCTACCT GTCGTCGGGC 
ATCACCTCCC GAGACTCATC TGAGCATGAA AAGAGAGCAA CTCTGAGCGC AGTGAAAGAG 
GAGAACCGGC ACCTCAACAA CTCCGTCATG ATCCTCACCT ACGCTCTCAT GAACGATGGA 
GTGACTGGAC TGAAGGAGCT GGCATTCTTG CGAGACCTGG CTGAGCAGAA TTCCCTGAAG 
TACGGCGTTC AGGATCGATC TGCACTGCCC GTGGTAAAGG GAAGTATGAT GGTTCTGAAC 
CAGCTCAGTA ATCTGGAGAC CACAGTGGGC CGCTTCTACA TCAACCTCCC CAACCGAATG 
ATCGACGTGG CCACCTTCAG CTTGCCTTAT GCGGACCAGA TGGGAGACGG TTTTATCATG 
ACCGTCAGCC GGCCGTGCTA CTTTGGTAAC TTGTTACTGG GTGTGGTTGG AGTGGATGTG 
AACCTGGCAT ACATACTGGA GGACGTTACC TACTACCAGG ACTCCCTGGC CTCATATACA 
TTCCTCATAG ACAACAAAGG CTACACTCTC ATGCATCCAT CACTCACCCG TCCTTACCTG 
ATGACTGAAG CGCCTCTTCA TACAGACATC ATCCACTATG AGAACATCCC AGGGTTTCCT 
GTTGTGCGTC AGAACATCCT CAGTCTGCCT CTGGGGAGTC AGGTCATTGC TGTGCCTGTC 
AACTCTTCTC TGTCTTGGCA CATGAACCGC CTCCGTGATC CTAATCGAGA TGCCTACAAT 
GTTAGCTATG CTTGGAAACT GGTTCAGGAC ACTTCGTTTA TCCTCTGCAT AGTTTATGTT 
CAGCCAGAGA TCCCGGTGAA GCAGCTGAAA AATCTGAACA CAGCCCCCAG CAGCAAACTT 
CTCTACCACC GCCTGGATCT GCTGGGGCAG CCCAGCTCCT GCCTTCACTT TAAACAGCTC 
GCTACAGTCG AGAGCCCTAC GGTGATGTTG TCCGCTGGTA GTTTCTCCTC TCCCTATGAG 
CACCTGAGTC AACCAGAGAC CAAGCGTATG GTGGAGCACT ACACTGCTTA CCTGAGCGAC 
AACACTCGAC TTATAGCCAA CCCCGGCCTG AAGTCTTCTG TGAGGAATGA GGTGATGGCC 
ACCAGCCACG TGACTGATGA ATGGATGAGT CTTATGGAGA TGAGCAGCCT CAACTGCTAC 
ATTGTGAGGC GTTACATAGC AACGCCCAAT GGGGTGCTGC GGATTTACCC CGGTTCACTC 
ATGGACAAAG CCTTCGATCC CACCAGGAGG CAATGGTACC TACATGCTGT AGCCAATCCT 
GGTCTCATCA CTTTTACTGG ACCGTACCTG GATGTTGGTG GGGCAGGTTA TGTAGTCACC 
ATTAGCCACA CCATCCACGC CCCTAGTTCC CAAATGGCCT CTGGTTACAC AGTAGCGGTA 
ATGGGCATTG ACTTCACCCT GCGCTACTTC TACAAAGTTC TGTTGGATCT TCTTCCCATC 
TGCAACCAGG ACAAAGGCAA CAAAATTCGG TGTTTTATCA TGGAGGACAG AGGCTATTTG 
GTGGCCCATC CGACACTGAT TGATCCGAAG GGTCACGCTC CTGCAGAACA ACAGCATATT 
ACGCATAAGG AGCCTCTAGT GGCCAATGAT ATTCTCAACC ACCCCAATTT TGTGAAGAAA 
AACCTGTGTA ACAGTTTCAG CGATCGCACT GTGCAGCGTT TCTACAAGTT CAACACAAGC 
ATCGTGGGTG ATCTGACAAA CCTTGTGCAT GGCAGTCACT GCTCAAAGTA CCGGCTTACT 
CGTATCCCAG GAACGAATGC GTTTGCTGGT ATTGTGAATG AGACGTGTGA TGCGCTGGCC 
TTCTGTGCTT GCAGTACTGT GGACCGTCTC TGCCTCAACT GCCACAGAAT GGAGCAGAAT 
GAATGTGAGT GCCCCTGTGA ATGCCCTCTA GAGGTGAACG AGTGTACTGG CAACCTCACC 
AACGCTGAGA ACAGAAATCC GAGCTGTGAC GTGCACCAGG AGCCTTTGTC TTTGACTGTA 
ATTGAGCCCA GTCTACAGGA TACTCTGCCA CAGTGCATCA ACACCAGATG CAGCCAGCGC 
TTTACCAGCA GTGACTGTTT TGGTGTTCTG GACTGTGAGT GGTGTATGGT GGACAGCGAT 
GGAAAGACTC ACCTGGATAA GCCTTACTGT GCCCTGCAGA AGGAGTGTTT CGGAGGCATA 
GTGGGCGCCA AGAGTCCTTA TGTGGATGGA ATGGGCATTT TAGATGAGGA GTTGGCTTCG 
CTGAACATGA TCAAGAGTGC TCCTGTGGGC CCTGTGGCTG GAGGGATTAT GGGATGCATC 
ATGGTTTTGG TGTTGGCAGT TTATGCCTAT AGGCACCAGA TCCACCGTCG CAGTCATCAG 
CACATGTCAC CGCTGGCCGC ACAGGAAATG TCTGTGCGGA TGTCCAACCT GGACAACGAG 
CGAGATGACA GAGATGAAGA CAGCCACGAA GACAGAGGAA TAATCAGCAA CACTCGATTC 
ATTGCTGCAG TTATTGAGAG GCACACACAC AGTCCAGAAC GACGGCGGAG ATACTGGGGC 
CGGTCAGGGA CTGAAAGTGA CCACGGTTAC AGTACCATGA GCCCTCAGGA GGACAGTGAG 
AACCCGCCCT GCAATAACGA TCCTCTCTCA GCGGGCGTTG ATGTGGGCAA TCACGACGAG 
GACCTTGACC TGGACACTCC TCCACAGACA GCTGCTCTTC TCAGCCACAA ATTCCACCCC 
TATCGGCACC CCCACATGCA CCACCCCCTG CATCTGCACA CACACCACCT TCAGGCCGCC 
GTAACCGTTC ACAGTGTGGA CGCTGAGTGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001334829.3
CDS	296..4168
Translation	MASRSRTGPRFSFAAWSKHSRPNWNNLEISKWSLVALLVFACCGFCGADSEFSILEEAQVLAVQMKKLSTQELGVLTMQRIFNSFVYTEKTSNGETEVQQLAKKIREKFNRYLDVVNRNKQVVEASYTAHLTSPLTAIQDCCSIPPSMMEFDGNFNANVSKTVSCDRLSPTVNSRAFNPGRDLNSVLADNLKSNPGIKWQYFSSEEGIFTLFPAHKFHCKGTFEHRSRPVYVAAVRPQSKHIVVIIDHGASVTETQLQIARDAALVILNSIDEHDKISVLTIADTVRSCSLDQCYKSFLSPATSETKRKMSTFISNIKASDSSTQHAVGFQKAFQLLRNTSNVTRQLTNTDMMIIYLSSGITSRDSSEHEKRATLSAVKEENRHLNNSVMILTYALMNDGVTGLKELAFLRDLAEQNSLKYGVQDRSALPVVKGSMMVLNQLSNLETTVGRFYINLPNRMIDVATFSLPYADQMGDGFIMTVSRPCYFGNLLLGVVGVDVNLAYILEDVTYYQDSLASYTFLIDNKGYTLMHPSLTRPYLMTEAPLHTDIIHYENIPGFPVVRQNILSLPLGSQVIAVPVNSSLSWHMNRLRDPNRDAYNVSYAWKLVQDTSFILCIVYVQPEIPVKQLKNLNTAPSSKLLYHRLDLLGQPSSCLHFKQLATVESPTVMLSAGSFSSPYEHLSQPETKRMVEHYTAYLSDNTRLIANPGLKSSVRNEVMATSHVTDEWMSLMEMSSLNCYIVRRYIATPNGVLRIYPGSLMDKAFDPTRRQWYLHAVANPGLITFTGPYLDVGGAGYVVTISHTIHAPSSQMASGYTVAVMGIDFTLRYFYKVLLDLLPICNQDKGNKIRCFIMEDRGYLVAHPTLIDPKGHAPAEQQHITHKEPLVANDILNHPNFVKKNLCNSFSDRTVQRFYKFNTSIVGDLTNLVHGSHCSKYRLTRIPGTNAFAGIVNETCDALAFCACSTVDRLCLNCHRMEQNECECPCECPLEVNECTGNLTNAENRNPSCDVHQEPLSLTVIEPSLQDTLPQCINTRCSQRFTSSDCFGVLDCEWCMVDSDGKTHLDKPYCALQKECFGGIVGAKSPYVDGMGILDEELASLNMIKSAPVGPVAGGIMGCIMVLVLAVYAYRHQIHRRSHQHMSPLAAQEMSVRMSNLDNERDDRDEDSHEDRGIISNTRFIAAVIERHTHSPERRRRYWGRSGTESDHGYSTMSPQEDSENPPCNNDPLSAGVDVGNHDEDLDLDTPPQTAALLSHKFHPYRHPHMHHPLHLHTHHLQAAVTVHSVDAEC

Target ORF information:

RefSeq Version	XM_001334793.6
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio cache domain containing 1 (cachd1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001334793.6

ORF Insert Sequence:

ATGGCGAGTC GGTCGCGGAC AGGACCTCGA TTCTCCTTCG CGGCGTGGAG CAAACACTCC 
CGACCAAACT GGAATAACCT GGAGATCTCA AAGTGGAGCC TAGTCGCCCT GCTGGTGTTT 
GCTTGTTGTG GATTTTGTGG CGCTGATAGT GAGTTTTCGA TCCTGGAAGA GGCTCAAGTT 
TTGGCCGTGC AGATGAAGAA GCTTTCGACT CAGGAGCTCG GTGTTCTCAC CATGCAGAGA 
ATCTTCAACT CCTTTGTTTA TACAGAGAAG ACTTCAAATG GTGAAACAGA GGTGCAGCAG 
CTAGCGAAAA AGATCCGAGA GAAGTTTAAC CGGTACCTGG ATGTGGTGAA CAGGAACAAG 
CAGGTGGTGG AAGCTTCATA CACGGCCCAC CTCACTTCAC CTCTCACAGC CATACAGGAC 
TGCTGCTCCA TCCCTCCCTC CATGATGGAA TTTGACGGTA ATTTTAATGC CAATGTTTCC 
AAGACCGTCA GCTGTGATCG TCTCTCCCCT ACCGTCAATA GCAGAGCTTT CAACCCAGGA 
CGAGACCTCA ACTCTGTGCT GGCAGACAAC CTGAAGTCCA ATCCAGGGAT CAAGTGGCAG 
TACTTCAGCT CAGAGGAGGG CATTTTCACT CTGTTTCCTG CTCACAAGTT CCACTGCAAG 
GGCACTTTTG AGCACAGGAG CAGGCCTGTG TATGTCGCGG CAGTAAGGCC ACAATCCAAG 
CATATCGTGG TGATTATAGA CCATGGTGCA TCTGTCACCG AAACCCAGCT CCAGATTGCT 
CGAGATGCTG CTCTGGTCAT CCTCAACTCC ATCGACGAAC ATGATAAAAT CTCAGTGTTG 
ACGATAGCAG ATACTGTTCG CTCCTGCTCT CTGGATCAGT GCTATAAGAG CTTCCTCTCA 
CCTGCTACCA GCGAGACTAA GAGGAAGATG AGCACCTTCA TCAGCAACAT TAAAGCTTCT 
GACAGCTCTA CCCAGCATGC AGTGGGCTTC CAGAAGGCCT TTCAGTTGTT GAGGAATACA 
AGCAACGTCA CCAGACAGCT GACTAACACT GACATGATGA TAATCTACCT GTCGTCGGGC 
ATCACCTCCC GAGACTCATC TGAGCATGAA AAGAGAGCAA CTCTGAGCGC AGTGAAAGAG 
GAGAACCGGC ACCTCAACAA CTCCGTCATG ATCCTCACCT ACGCTCTCAT GAACGATGGA 
GTGACTGGAC TGAAGGAGCT GGCATTCTTG CGAGACCTGG CTGAGCAGAA TTCCCTGAAG 
TACGGCGTTC AGGATCGATC TGCACTGCCC GTGGTAAAGG GAAGTATGAT GGTTCTGAAC 
CAGCTCAGTA ATCTGGAGAC CACAGTGGGC CGCTTCTACA TCAACCTCCC CAACCGAATG 
ATCGACGTGG CCACCTTCAG CTTGCCTTAT GCGGACCAGA TGGGAGACGG TTTTATCATG 
ACCGTCAGCC GGCCGTGCTA CTTTGGTAAC TTGTTACTGG GTGTGGTTGG AGTGGATGTG 
AACCTGGCAT ACATACTGGA GGACGTTACC TACTACCAGG ACTCCCTGGC CTCATATACA 
TTCCTCATAG ACAACAAAGG CTACACTCTC ATGCATCCAT CACTCACCCG TCCTTACCTG 
ATGACTGAAG CGCCTCTTCA TACAGACATC ATCCACTATG AGAACATCCC AGGGTTTCCT 
GTTGTGCGTC AGAACATCCT CAGTCTGCCT CTGGGGAGTC AGGTCATTGC TGTGCCTGTC 
AACTCTTCTC TGTCTTGGCA CATGAACCGC CTCCGTGATC CTAATCGAGA TGCCTACAAT 
GTTAGCTATG CTTGGAAACT GGTTCAGGAC ACTTCGTTTA TCCTCTGCAT AGTTTATGTT 
CAGCCAGAGA TCCCGGTGAA GCAGCTGAAA AATCTGAACA CAGCCCCCAG CAGCAAACTT 
CTCTACCACC GCCTGGATCT GCTGGGGCAG CCCAGCTCCT GCCTTCACTT TAAACAGCTC 
GCTACAGTCG AGAGCCCTAC GGTGATGTTG TCCGCTGGTA GTTTCTCCTC TCCCTATGAG 
CACCTGAGTC AACCAGAGAC CAAGCGTATG GTGGAGCACT ACACTGCTTA CCTGAGCGAC 
AACACTCGAC TTATAGCCAA CCCCGGCCTG AAGTCTTCTG TGAGGAATGA GGTGATGGCC 
ACCAGCCACG TGACTGATGA ATGGATGAGT CTTATGGAGA TGAGCAGCCT CAACTGCTAC 
ATTGTGAGGC GTTACATAGC AACGCCCAAT GGGGTGCTGC GGATTTACCC CGGTTCACTC 
ATGGACAAAG CCTTCGATCC CACCAGGAGG CAATGGTACC TACATGCTGT AGCCAATCCT 
GGTCTCATCA CTTTTACTGG ACCGTACCTG GATGTTGGTG GGGCAGGTTA TGTAGTCACC 
ATTAGCCACA CCATCCACGC CCCTAGTTCC CAAATGGCCT CTGGTTACAC AGTAGCGGTA 
ATGGGCATTG ACTTCACCCT GCGCTACTTC TACAAAGTTC TGTTGGATCT TCTTCCCATC 
TGCAACCAGG ACAAAGGCAA CAAAATTCGG TGTTTTATCA TGGAGGACAG AGGCTATTTG 
GTGGCCCATC CGACACTGAT TGATCCGAAG GGTCACGCTC CTGCAGAACA ACAGCATATT 
ACGCATAAGG AGCCTCTAGT GGCCAATGAT ATTCTCAACC ACCCCAATTT TGTGAAGAAA 
AACCTGTGTA ACAGTTTCAG CGATCGCACT GTGCAGCGTT TCTACAAGTT CAACACAAGC 
ATCGTGGGTG ATCTGACAAA CCTTGTGCAT GGCAGTCACT GCTCAAAGTA CCGGCTTACT 
CGTATCCCAG GAACGAATGC GTTTGCTGGT ATTGTGAATG AGACGTGTGA TGCGCTGGCC 
TTCTGTGCTT GCAGTACTGT GGACCGTCTC TGCCTCAACT GCCACAGAAT GGAGCAGAAT 
GAATGTGAGT GCCCCTGTGA ATGCCCTCTA GAGGTGAACG AGTGTACTGG CAACCTCACC 
AACGCTGAGA ACAGAAATCC GAGCTGTGAC GTGCACCAGG AGCCTTTGTC TTTGACTGTA 
ATTGAGCCCA GTCTACAGGA TACTCTGCCA CAGTGCATCA ACACCAGATG CAGCCAGCGC 
TTTACCAGCA GTGACTGTTT TGGTGTTCTG GACTGTGAGT GGTGTATGGT GGACAGCGAT 
GGAAAGACTC ACCTGGATAA GCCTTACTGT GCCCTGCAGA AGGAGTGTTT CGGAGGCATA 
GTGGGCGCCA AGAGTCCTTA TGTGGATGGA ATGGGCATTT TAGATGAGGA GTTGGCTTCG 
CTGAACATGA TCAAGAGTGC TCCTGTGGGC CCTGTGGCTG GAGGGATTAT GGGATGCATC 
ATGGTTTTGG TGTTGGCAGT TTATGCCTAT AGGCACCAGA TCCACCGTCG CAGTCATCAG 
CACATGTCAC CGCTGGCCGC ACAGGAAATG TCTGTGCGGA TGTCCAACCT GGACAACGAG 
CGAGATGACA GAGATGAAGA CAGCCACGAA GACAGAGGAA TAATCAGCAA CACTCGATTC 
ATTGCTGCAG TTATTGAGAG GCACACACAC AGTCCAGAAC GACGGCGGAG ATACTGGGGC 
CGGTCAGGGA CTGAAAGTGA CCACGGTTAC AGTACCATGA GCCCTCAGGA GGACAGTGAG 
AACCCGCCCT GCAATAACGA TCCTCTCTCA GCGGGCGTTG ATGTGGGCAA TCACGACGAG 
GACCTTGACC TGGACACTCC TCCACAGACA GCTGCTCTTC TCAGCCACAA ATTCCACCCC 
TATCGGCACC CCCACATGCA CCACCCCCTG CATCTGCACA CACACCACCT TCAGGCCGCC 
GTAACCGTTC ACAGTGTGGA CGCTGAGTGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cachd1 ( XM_001334793.7 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_001334829.3 Danio rerio cache domain containing 1 (cachd1), transcript variant X1, mRNA.

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CloneID	ODa11929
Clone ID Related Accession (Same CDS sequence)	XM_001334793.6 , XM_001334793.7
Accession Version	XM_001334793.7 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3873bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	VWFA and cache domain-containing protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_001334793.6. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAGTC GGTCGCGGAC AGGACCTCGA TTCTCCTTCG CGGCGTGGAG CAAACACTCC 
CGACCAAACT GGAATAACCT GGAGATCTCA AAGTGGAGCC TAGTCGCCCT GCTGGTGTTT 
GCTTGTTGTG GATTTTGTGG CGCTGATAGT GAGTTTTCGA TCCTGGAAGA GGCTCAAGTT 
TTGGCCGTGC AGATGAAGAA GCTTTCGACT CAGGAGCTCG GTGTTCTCAC CATGCAGAGA 
ATCTTCAACT CCTTTGTTTA TACAGAGAAG ACTTCAAATG GTGAAACAGA GGTGCAGCAG 
CTAGCGAAAA AGATCCGAGA GAAGTTTAAC CGGTACCTGG ATGTGGTGAA CAGGAACAAG 
CAGGTGGTGG AAGCTTCATA CACGGCCCAC CTCACTTCAC CTCTCACAGC CATACAGGAC 
TGCTGCTCCA TCCCTCCCTC CATGATGGAA TTTGACGGTA ATTTTAATGC CAATGTTTCC 
AAGACCGTCA GCTGTGATCG TCTCTCCCCT ACCGTCAATA GCAGAGCTTT CAACCCAGGA 
CGAGACCTCA ACTCTGTGCT GGCAGACAAC CTGAAGTCCA ATCCAGGGAT CAAGTGGCAG 
TACTTCAGCT CAGAGGAGGG CATTTTCACT CTGTTTCCTG CTCACAAGTT CCACTGCAAG 
GGCACTTTTG AGCACAGGAG CAGGCCTGTG TATGTCGCGG CAGTAAGGCC ACAATCCAAG 
CATATCGTGG TGATTATAGA CCATGGTGCA TCTGTCACCG AAACCCAGCT CCAGATTGCT 
CGAGATGCTG CTCTGGTCAT CCTCAACTCC ATCGACGAAC ATGATAAAAT CTCAGTGTTG 
ACGATAGCAG ATACTGTTCG CTCCTGCTCT CTGGATCAGT GCTATAAGAG CTTCCTCTCA 
CCTGCTACCA GCGAGACTAA GAGGAAGATG AGCACCTTCA TCAGCAACAT TAAAGCTTCT 
GACAGCTCTA CCCAGCATGC AGTGGGCTTC CAGAAGGCCT TTCAGTTGTT GAGGAATACA 
AGCAACGTCA CCAGACAGCT GACTAACACT GACATGATGA TAATCTACCT GTCGTCGGGC 
ATCACCTCCC GAGACTCATC TGAGCATGAA AAGAGAGCAA CTCTGAGCGC AGTGAAAGAG 
GAGAACCGGC ACCTCAACAA CTCCGTCATG ATCCTCACCT ACGCTCTCAT GAACGATGGA 
GTGACTGGAC TGAAGGAGCT GGCATTCTTG CGAGACCTGG CTGAGCAGAA TTCCCTGAAG 
TACGGCGTTC AGGATCGATC TGCACTGCCC GTGGTAAAGG GAAGTATGAT GGTTCTGAAC 
CAGCTCAGTA ATCTGGAGAC CACAGTGGGC CGCTTCTACA TCAACCTCCC CAACCGAATG 
ATCGACGTGG CCACCTTCAG CTTGCCTTAT GCGGACCAGA TGGGAGACGG TTTTATCATG 
ACCGTCAGCC GGCCGTGCTA CTTTGGTAAC TTGTTACTGG GTGTGGTTGG AGTGGATGTG 
AACCTGGCAT ACATACTGGA GGACGTTACC TACTACCAGG ACTCCCTGGC CTCATATACA 
TTCCTCATAG ACAACAAAGG CTACACTCTC ATGCATCCAT CACTCACCCG TCCTTACCTG 
ATGACTGAAG CGCCTCTTCA TACAGACATC ATCCACTATG AGAACATCCC AGGGTTTCCT 
GTTGTGCGTC AGAACATCCT CAGTCTGCCT CTGGGGAGTC AGGTCATTGC TGTGCCTGTC 
AACTCTTCTC TGTCTTGGCA CATGAACCGC CTCCGTGATC CTAATCGAGA TGCCTACAAT 
GTTAGCTATG CTTGGAAACT GGTTCAGGAC ACTTCGTTTA TCCTCTGCAT AGTTTATGTT 
CAGCCAGAGA TCCCGGTGAA GCAGCTGAAA AATCTGAACA CAGCCCCCAG CAGCAAACTT 
CTCTACCACC GCCTGGATCT GCTGGGGCAG CCCAGCTCCT GCCTTCACTT TAAACAGCTC 
GCTACAGTCG AGAGCCCTAC GGTGATGTTG TCCGCTGGTA GTTTCTCCTC TCCCTATGAG 
CACCTGAGTC AACCAGAGAC CAAGCGTATG GTGGAGCACT ACACTGCTTA CCTGAGCGAC 
AACACTCGAC TTATAGCCAA CCCCGGCCTG AAGTCTTCTG TGAGGAATGA GGTGATGGCC 
ACCAGCCACG TGACTGATGA ATGGATGAGT CTTATGGAGA TGAGCAGCCT CAACTGCTAC 
ATTGTGAGGC GTTACATAGC AACGCCCAAT GGGGTGCTGC GGATTTACCC CGGTTCACTC 
ATGGACAAAG CCTTCGATCC CACCAGGAGG CAATGGTACC TACATGCTGT AGCCAATCCT 
GGTCTCATCA CTTTTACTGG ACCGTACCTG GATGTTGGTG GGGCAGGTTA TGTAGTCACC 
ATTAGCCACA CCATCCACGC CCCTAGTTCC CAAATGGCCT CTGGTTACAC AGTAGCGGTA 
ATGGGCATTG ACTTCACCCT GCGCTACTTC TACAAAGTTC TGTTGGATCT TCTTCCCATC 
TGCAACCAGG ACAAAGGCAA CAAAATTCGG TGTTTTATCA TGGAGGACAG AGGCTATTTG 
GTGGCCCATC CGACACTGAT TGATCCGAAG GGTCACGCTC CTGCAGAACA ACAGCATATT 
ACGCATAAGG AGCCTCTAGT GGCCAATGAT ATTCTCAACC ACCCCAATTT TGTGAAGAAA 
AACCTGTGTA ACAGTTTCAG CGATCGCACT GTGCAGCGTT TCTACAAGTT CAACACAAGC 
ATCGTGGGTG ATCTGACAAA CCTTGTGCAT GGCAGTCACT GCTCAAAGTA CCGGCTTACT 
CGTATCCCAG GAACGAATGC GTTTGCTGGT ATTGTGAATG AGACGTGTGA TGCGCTGGCC 
TTCTGTGCTT GCAGTACTGT GGACCGTCTC TGCCTCAACT GCCACAGAAT GGAGCAGAAT 
GAATGTGAGT GCCCCTGTGA ATGCCCTCTA GAGGTGAACG AGTGTACTGG CAACCTCACC 
AACGCTGAGA ACAGAAATCC GAGCTGTGAC GTGCACCAGG AGCCTTTGTC TTTGACTGTA 
ATTGAGCCCA GTCTACAGGA TACTCTGCCA CAGTGCATCA ACACCAGATG CAGCCAGCGC 
TTTACCAGCA GTGACTGTTT TGGTGTTCTG GACTGTGAGT GGTGTATGGT GGACAGCGAT 
GGAAAGACTC ACCTGGATAA GCCTTACTGT GCCCTGCAGA AGGAGTGTTT CGGAGGCATA 
GTGGGCGCCA AGAGTCCTTA TGTGGATGGA ATGGGCATTT TAGATGAGGA GTTGGCTTCG 
CTGAACATGA TCAAGAGTGC TCCTGTGGGC CCTGTGGCTG GAGGGATTAT GGGATGCATC 
ATGGTTTTGG TGTTGGCAGT TTATGCCTAT AGGCACCAGA TCCACCGTCG CAGTCATCAG 
CACATGTCAC CGCTGGCCGC ACAGGAAATG TCTGTGCGGA TGTCCAACCT GGACAACGAG 
CGAGATGACA GAGATGAAGA CAGCCACGAA GACAGAGGAA TAATCAGCAA CACTCGATTC 
ATTGCTGCAG TTATTGAGAG GCACACACAC AGTCCAGAAC GACGGCGGAG ATACTGGGGC 
CGGTCAGGGA CTGAAAGTGA CCACGGTTAC AGTACCATGA GCCCTCAGGA GGACAGTGAG 
AACCCGCCCT GCAATAACGA TCCTCTCTCA GCGGGCGTTG ATGTGGGCAA TCACGACGAG 
GACCTTGACC TGGACACTCC TCCACAGACA GCTGCTCTTC TCAGCCACAA ATTCCACCCC 
TATCGGCACC CCCACATGCA CCACCCCCTG CATCTGCACA CACACCACCT TCAGGCCGCC 
GTAACCGTTC ACAGTGTGGA CGCTGAGTGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001334829.3
CDS	355..4227
Translation	MASRSRTGPRFSFAAWSKHSRPNWNNLEISKWSLVALLVFACCGFCGADSEFSILEEAQVLAVQMKKLSTQELGVLTMQRIFNSFVYTEKTSNGETEVQQLAKKIREKFNRYLDVVNRNKQVVEASYTAHLTSPLTAIQDCCSIPPSMMEFDGNFNANVSKTVSCDRLSPTVNSRAFNPGRDLNSVLADNLKSNPGIKWQYFSSEEGIFTLFPAHKFHCKGTFEHRSRPVYVAAVRPQSKHIVVIIDHGASVTETQLQIARDAALVILNSIDEHDKISVLTIADTVRSCSLDQCYKSFLSPATSETKRKMSTFISNIKASDSSTQHAVGFQKAFQLLRNTSNVTRQLTNTDMMIIYLSSGITSRDSSEHEKRATLSAVKEENRHLNNSVMILTYALMNDGVTGLKELAFLRDLAEQNSLKYGVQDRSALPVVKGSMMVLNQLSNLETTVGRFYINLPNRMIDVATFSLPYADQMGDGFIMTVSRPCYFGNLLLGVVGVDVNLAYILEDVTYYQDSLASYTFLIDNKGYTLMHPSLTRPYLMTEAPLHTDIIHYENIPGFPVVRQNILSLPLGSQVIAVPVNSSLSWHMNRLRDPNRDAYNVSYAWKLVQDTSFILCIVYVQPEIPVKQLKNLNTAPSSKLLYHRLDLLGQPSSCLHFKQLATVESPTVMLSAGSFSSPYEHLSQPETKRMVEHYTAYLSDNTRLIANPGLKSSVRNEVMATSHVTDEWMSLMEMSSLNCYIVRRYIATPNGVLRIYPGSLMDKAFDPTRRQWYLHAVANPGLITFTGPYLDVGGAGYVVTISHTIHAPSSQMASGYTVAVMGIDFTLRYFYKVLLDLLPICNQDKGNKIRCFIMEDRGYLVAHPTLIDPKGHAPAEQQHITHKEPLVANDILNHPNFVKKNLCNSFSDRTVQRFYKFNTSIVGDLTNLVHGSHCSKYRLTRIPGTNAFAGIVNETCDALAFCACSTVDRLCLNCHRMEQNECECPCECPLEVNECTGNLTNAENRNPSCDVHQEPLSLTVIEPSLQDTLPQCINTRCSQRFTSSDCFGVLDCEWCMVDSDGKTHLDKPYCALQKECFGGIVGAKSPYVDGMGILDEELASLNMIKSAPVGPVAGGIMGCIMVLVLAVYAYRHQIHRRSHQHMSPLAAQEMSVRMSNLDNERDDRDEDSHEDRGIISNTRFIAAVIERHTHSPERRRRYWGRSGTESDHGYSTMSPQEDSENPPCNNDPLSAGVDVGNHDEDLDLDTPPQTAALLSHKFHPYRHPHMHHPLHLHTHHLQAAVTVHSVDAEC

Target ORF information:

RefSeq Version	XM_001334793.7
Organism	Danio rerio(zebrafish)
Definition	Danio rerio cache domain containing 1 (cachd1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001334793.7

ORF Insert Sequence:

ATGGCGAGTC GGTCGCGGAC AGGACCTCGA TTCTCCTTCG CGGCGTGGAG CAAACACTCC 
CGACCAAACT GGAATAACCT GGAGATCTCA AAGTGGAGCC TAGTCGCCCT GCTGGTGTTT 
GCTTGTTGTG GATTTTGTGG CGCTGATAGT GAGTTTTCGA TCCTGGAAGA GGCTCAAGTT 
TTGGCCGTGC AGATGAAGAA GCTTTCGACT CAGGAGCTCG GTGTTCTCAC CATGCAGAGA 
ATCTTCAACT CCTTTGTTTA TACAGAGAAG ACTTCAAATG GTGAAACAGA GGTGCAGCAG 
CTAGCGAAAA AGATCCGAGA GAAGTTTAAC CGGTACCTGG ATGTGGTGAA CAGGAACAAG 
CAGGTGGTGG AAGCTTCATA CACGGCCCAC CTCACTTCAC CTCTCACAGC CATACAGGAC 
TGCTGCTCCA TCCCTCCCTC CATGATGGAA TTTGACGGTA ATTTTAATGC CAATGTTTCC 
AAGACCGTCA GCTGTGATCG TCTCTCCCCT ACCGTCAATA GCAGAGCTTT CAACCCAGGA 
CGAGACCTCA ACTCTGTGCT GGCAGACAAC CTGAAGTCCA ATCCAGGGAT CAAGTGGCAG 
TACTTCAGCT CAGAGGAGGG CATTTTCACT CTGTTTCCTG CTCACAAGTT CCACTGCAAG 
GGCACTTTTG AGCACAGGAG CAGGCCTGTG TATGTCGCGG CAGTAAGGCC ACAATCCAAG 
CATATCGTGG TGATTATAGA CCATGGTGCA TCTGTCACCG AAACCCAGCT CCAGATTGCT 
CGAGATGCTG CTCTGGTCAT CCTCAACTCC ATCGACGAAC ATGATAAAAT CTCAGTGTTG 
ACGATAGCAG ATACTGTTCG CTCCTGCTCT CTGGATCAGT GCTATAAGAG CTTCCTCTCA 
CCTGCTACCA GCGAGACTAA GAGGAAGATG AGCACCTTCA TCAGCAACAT TAAAGCTTCT 
GACAGCTCTA CCCAGCATGC AGTGGGCTTC CAGAAGGCCT TTCAGTTGTT GAGGAATACA 
AGCAACGTCA CCAGACAGCT GACTAACACT GACATGATGA TAATCTACCT GTCGTCGGGC 
ATCACCTCCC GAGACTCATC TGAGCATGAA AAGAGAGCAA CTCTGAGCGC AGTGAAAGAG 
GAGAACCGGC ACCTCAACAA CTCCGTCATG ATCCTCACCT ACGCTCTCAT GAACGATGGA 
GTGACTGGAC TGAAGGAGCT GGCATTCTTG CGAGACCTGG CTGAGCAGAA TTCCCTGAAG 
TACGGCGTTC AGGATCGATC TGCACTGCCC GTGGTAAAGG GAAGTATGAT GGTTCTGAAC 
CAGCTCAGTA ATCTGGAGAC CACAGTGGGC CGCTTCTACA TCAACCTCCC CAACCGAATG 
ATCGACGTGG CCACCTTCAG CTTGCCTTAT GCGGACCAGA TGGGAGACGG TTTTATCATG 
ACCGTCAGCC GGCCGTGCTA CTTTGGTAAC TTGTTACTGG GTGTGGTTGG AGTGGATGTG 
AACCTGGCAT ACATACTGGA GGACGTTACC TACTACCAGG ACTCCCTGGC CTCATATACA 
TTCCTCATAG ACAACAAAGG CTACACTCTC ATGCATCCAT CACTCACCCG TCCTTACCTG 
ATGACTGAAG CGCCTCTTCA TACAGACATC ATCCACTATG AGAACATCCC AGGGTTTCCT 
GTTGTGCGTC AGAACATCCT CAGTCTGCCT CTGGGGAGTC AGGTCATTGC TGTGCCTGTC 
AACTCTTCTC TGTCTTGGCA CATGAACCGC CTCCGTGATC CTAATCGAGA TGCCTACAAT 
GTTAGCTATG CTTGGAAACT GGTTCAGGAC ACTTCGTTTA TCCTCTGCAT AGTTTATGTT 
CAGCCAGAGA TCCCGGTGAA GCAGCTGAAA AATCTGAACA CAGCCCCCAG CAGCAAACTT 
CTCTACCACC GCCTGGATCT GCTGGGGCAG CCCAGCTCCT GCCTTCACTT TAAACAGCTC 
GCTACAGTCG AGAGCCCTAC GGTGATGTTG TCCGCTGGTA GTTTCTCCTC TCCCTATGAG 
CACCTGAGTC AACCAGAGAC CAAGCGTATG GTGGAGCACT ACACTGCTTA CCTGAGCGAC 
AACACTCGAC TTATAGCCAA CCCCGGCCTG AAGTCTTCTG TGAGGAATGA GGTGATGGCC 
ACCAGCCACG TGACTGATGA ATGGATGAGT CTTATGGAGA TGAGCAGCCT CAACTGCTAC 
ATTGTGAGGC GTTACATAGC AACGCCCAAT GGGGTGCTGC GGATTTACCC CGGTTCACTC 
ATGGACAAAG CCTTCGATCC CACCAGGAGG CAATGGTACC TACATGCTGT AGCCAATCCT 
GGTCTCATCA CTTTTACTGG ACCGTACCTG GATGTTGGTG GGGCAGGTTA TGTAGTCACC 
ATTAGCCACA CCATCCACGC CCCTAGTTCC CAAATGGCCT CTGGTTACAC AGTAGCGGTA 
ATGGGCATTG ACTTCACCCT GCGCTACTTC TACAAAGTTC TGTTGGATCT TCTTCCCATC 
TGCAACCAGG ACAAAGGCAA CAAAATTCGG TGTTTTATCA TGGAGGACAG AGGCTATTTG 
GTGGCCCATC CGACACTGAT TGATCCGAAG GGTCACGCTC CTGCAGAACA ACAGCATATT 
ACGCATAAGG AGCCTCTAGT GGCCAATGAT ATTCTCAACC ACCCCAATTT TGTGAAGAAA 
AACCTGTGTA ACAGTTTCAG CGATCGCACT GTGCAGCGTT TCTACAAGTT CAACACAAGC 
ATCGTGGGTG ATCTGACAAA CCTTGTGCAT GGCAGTCACT GCTCAAAGTA CCGGCTTACT 
CGTATCCCAG GAACGAATGC GTTTGCTGGT ATTGTGAATG AGACGTGTGA TGCGCTGGCC 
TTCTGTGCTT GCAGTACTGT GGACCGTCTC TGCCTCAACT GCCACAGAAT GGAGCAGAAT 
GAATGTGAGT GCCCCTGTGA ATGCCCTCTA GAGGTGAACG AGTGTACTGG CAACCTCACC 
AACGCTGAGA ACAGAAATCC GAGCTGTGAC GTGCACCAGG AGCCTTTGTC TTTGACTGTA 
ATTGAGCCCA GTCTACAGGA TACTCTGCCA CAGTGCATCA ACACCAGATG CAGCCAGCGC 
TTTACCAGCA GTGACTGTTT TGGTGTTCTG GACTGTGAGT GGTGTATGGT GGACAGCGAT 
GGAAAGACTC ACCTGGATAA GCCTTACTGT GCCCTGCAGA AGGAGTGTTT CGGAGGCATA 
GTGGGCGCCA AGAGTCCTTA TGTGGATGGA ATGGGCATTT TAGATGAGGA GTTGGCTTCG 
CTGAACATGA TCAAGAGTGC TCCTGTGGGC CCTGTGGCTG GAGGGATTAT GGGATGCATC 
ATGGTTTTGG TGTTGGCAGT TTATGCCTAT AGGCACCAGA TCCACCGTCG CAGTCATCAG 
CACATGTCAC CGCTGGCCGC ACAGGAAATG TCTGTGCGGA TGTCCAACCT GGACAACGAG 
CGAGATGACA GAGATGAAGA CAGCCACGAA GACAGAGGAA TAATCAGCAA CACTCGATTC 
ATTGCTGCAG TTATTGAGAG GCACACACAC AGTCCAGAAC GACGGCGGAG ATACTGGGGC 
CGGTCAGGGA CTGAAAGTGA CCACGGTTAC AGTACCATGA GCCCTCAGGA GGACAGTGAG 
AACCCGCCCT GCAATAACGA TCCTCTCTCA GCGGGCGTTG ATGTGGGCAA TCACGACGAG 
GACCTTGACC TGGACACTCC TCCACAGACA GCTGCTCTTC TCAGCCACAA ATTCCACCCC 
TATCGGCACC CCCACATGCA CCACCCCCTG CATCTGCACA CACACCACCT TCAGGCCGCC 
GTAACCGTTC ACAGTGTGGA CGCTGAGTGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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cachd1 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

見積依頼

Eメール

電話

FAX

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