glra2 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	glra2
Entrez Gene ID	793646
Full Name	glycine receptor, alpha 2
General protein information	Preferred Names glycine receptor, alpha 2 Names glycine receptor subunit alpha-2
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021478777.1	XP_021334452.1	glycine receptor subunit alpha-2 isoform X1
NM_001167899.1	NP_001161371.1	glycine receptor subunit alpha-2 precursor

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	GLRA2	XP_001137132.1
	GLRA2	XP_001099570.1
Canis lupus familiaris (dog)	GLRA2	XP_548866.2
Rattus norvegicus (Norway rat)	LOC100910572	XP_003752087.1
Danio rerio (zebrafish)	glra2	NP_001161371.1
Xenopus tropicalis (tropical clawed frog)	glra2	XP_004912302.1
Bos taurus (cattle)	GLRA2	NP_001179843.1
Homo sapiens (human)	GLRA2	NP_002054.1
Rattus norvegicus (Norway rat)	Glra2	NP_036700.1
Gallus gallus (chicken)	GLRA2	XP_004934752.1
Mus musculus (house mouse)	Glra2	NP_906272.1

Gene Ontology
Bibliography

Related articles in PubMed

Genetic and functional analyses demonstrate a role for abnormal glycinergic signaling in autism.
Pilorge M, Fassier C, Le Corronc H, Potey A, Bai J, De Gois S, Delaby E, Assouline B, Guinchat V, Devillard F, Delorme R, Nygren G, R?stam M, Meier JC, Otani S, Cheval H, James VM, Topf M, Dear TN, Gillberg C, Leboyer M, Giros B, Gautron S, Hazan J, Harvey RJ, Legendre P, Betancur C
Molecular psychiatry21(7)936-45(2016 07)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

Defective escape behavior in DEAH-box RNA helicase mutants improved by restoring glycine receptor expression.
Hirata H, Ogino K, Yamada K, Leacock S, Harvey RJ
The Journal of neuroscience : the official journal of the Society for Neuroscience33(37)14638-44(2013 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following glra2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the glra2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa04407	NM_001167899.1 Latest version!	Danio rerio glycine receptor, alpha 2 (glra2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
ODa143998	XM_021478777.1 Latest version!	Danio rerio glycine receptor, alpha 2 (glra2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

glra2 ( NM_001167899.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001161371.1 Danio rerio glycine receptor, alpha 2 (glra2), mRNA.

Price & Availability↑

CloneID	ODa04407
Clone ID Related Accession (Same CDS sequence)	NM_001167899.1
Accession Version	NM_001167899.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1347bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-21
Organism	Danio rerio(zebrafish)
Product	glycine receptor subunit alpha-2 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from GQ406228.1. On Nov 20, 2009 this sequence version replaced XM_001333403.2. ##Evidence-Data-START## Transcript exon combination :: GQ406228.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168447, SAMEA2168448 [ECO:0000348] ##Evidence-Data-END##

ATGACTCGCC CTTCGGTCAA ACTCCTCACA ACTTTACTCG CGTGTCTGAT GGAAATGCTC 
AACTTCAGGG TATCGTCAGG GAAGGATCCG GATCTTCTGT CCTCCTCCTC CAGCATGTCT 
CCCTCTGACT TCCTGGATAA ACTGATGGGC AGAACGTCCG GCTATGACGC TCGCATTCGA 
CCAAACTTCA AAGGTCCTCC TGTGAACGTC ACGTGTAATA TATTCATCAA CAGCTTCGGT 
TCAGTCACAG AAACTACTAT GGACTACAGG GTGAACATTT TCCTCCGGCA GAAGTGGAAC 
GACCCTCGAC TGGCCTACAG CGAGTATCCG GACTCGTCTC TGGATCTGGA CCCGTCCATG 
CTGGACTCCA TCTGGAAGCC TGACCTGTTC TTCGCCAACG AGAAAGGAGC AAATTTTCAC 
GACGTCACGA CAGACAACAA ACTCCTGAGG ATCTTCAAGG ACGGAACCGT TCTGTACAGC 
ATCAGGCTGA CGTTAATACT GTCCTGTCCA ATGGATCTGA AGAATTTCCC TATGGATGTT 
CAGACGTGTA CCATGCAGCT GGAGAGCTTC GGATACACCA TGAATGACCT GATTTTTGAG 
TGGCTGGATA AAGGGCCCGT TCAGGTGGCT GATGGTCTGA CTTTACCTCA GTTCCTCATC 
CGGGACGAGA AAGACCTCGG ATACTGTACA AAACACTATA ACACAGGTAA GTTCACCTGT 
ATCGAGGTGA AGTTCCACCT GGAGCGGCAG ATGGGTTATT ACCTGATCCA GATGTACATC 
CCGAGTCTGC TGATCGTCAT CCTGTCCTGG GTTTCCTTCT GGATCAACAT GGACGCGGCT 
CCGGCTCGAG TGGCTTTGGG CATCACTACT GTCCTCACCA TGACAACCCA GAGCTCTGGG 
TCACGCGCAT CACTGCCAAA GGTCTCCTAT GTGAAGGCTA TAGACATCTG GATGGCGGTG 
TGTTTGCTGT TCGTCTTCGC TGCTCTTCTT GAATACGCCG GAGTAAACTT TGTCTCCAGA 
CAACAGAAAG AGTTTCTGCG CTTGAAACGA AGACAAAGAC GAACTCAAAA GGAGGAGGAT 
TTACAGGACG GACGTTTGCA TTTTTCCAGC TACAACACAA CGACCTGCGT AAAAGATGGC 
GCCGTGGTCA AAAACACACA GGTGAACCAA ATCCAGCAGC CGTCCATCCT GAAAAACACA 
GAAACAAACC GCAAGAAGTT TGTGGATCGA GCCAAACGAA TCGACACCAT ATCACGCGCC 
GCGTTCCCAC TGGCGTTCTT GATCTTCAAC GTTTTTTACT GGATCACGTA TAAGATCATC 
AGACACGAGA GCGCAAGAAA AGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001161371.1
CDS	1..1347
Translation	MTRPSVKLLTTLLACLMEMLNFRVSSGKDPDLLSSSSSMSPSDFLDKLMGRTSGYDARIRPNFKGPPVNVTCNIFINSFGSVTETTMDYRVNIFLRQKWNDPRLAYSEYPDSSLDLDPSMLDSIWKPDLFFANEKGANFHDVTTDNKLLRIFKDGTVLYSIRLTLILSCPMDLKNFPMDVQTCTMQLESFGYTMNDLIFEWLDKGPVQVADGLTLPQFLIRDEKDLGYCTKHYNTGKFTCIEVKFHLERQMGYYLIQMYIPSLLIVILSWVSFWINMDAAPARVALGITTVLTMTTQSSGSRASLPKVSYVKAIDIWMAVCLLFVFAALLEYAGVNFVSRQQKEFLRLKRRQRRTQKEEDLQDGRLHFSSYNTTTCVKDGAVVKNTQVNQIQQPSILKNTETNRKKFVDRAKRIDTISRAAFPLAFLIFNVFYWITYKIIRHESARKD

Target ORF information:

RefSeq Version	NM_001167899.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio glycine receptor, alpha 2 (glra2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001167899.1

ORF Insert Sequence:

ATGACTCGCC CTTCGGTCAA ACTCCTCACA ACTTTACTCG CGTGTCTGAT GGAAATGCTC 
AACTTCAGGG TATCGTCAGG GAAGGATCCG GATCTTCTGT CCTCCTCCTC CAGCATGTCT 
CCCTCTGACT TCCTGGATAA ACTGATGGGC AGAACGTCCG GCTATGACGC TCGCATTCGA 
CCAAACTTCA AAGGTCCTCC TGTGAACGTC ACGTGTAATA TATTCATCAA CAGCTTCGGT 
TCAGTCACAG AAACTACTAT GGACTACAGG GTGAACATTT TCCTCCGGCA GAAGTGGAAC 
GACCCTCGAC TGGCCTACAG CGAGTATCCG GACTCGTCTC TGGATCTGGA CCCGTCCATG 
CTGGACTCCA TCTGGAAGCC TGACCTGTTC TTCGCCAACG AGAAAGGAGC AAATTTTCAC 
GACGTCACGA CAGACAACAA ACTCCTGAGG ATCTTCAAGG ACGGAACCGT TCTGTACAGC 
ATCAGGCTGA CGTTAATACT GTCCTGTCCA ATGGATCTGA AGAATTTCCC TATGGATGTT 
CAGACGTGTA CCATGCAGCT GGAGAGCTTC GGATACACCA TGAATGACCT GATTTTTGAG 
TGGCTGGATA AAGGGCCCGT TCAGGTGGCT GATGGTCTGA CTTTACCTCA GTTCCTCATC 
CGGGACGAGA AAGACCTCGG ATACTGTACA AAACACTATA ACACAGGTAA GTTCACCTGT 
ATCGAGGTGA AGTTCCACCT GGAGCGGCAG ATGGGTTATT ACCTGATCCA GATGTACATC 
CCGAGTCTGC TGATCGTCAT CCTGTCCTGG GTTTCCTTCT GGATCAACAT GGACGCGGCT 
CCGGCTCGAG TGGCTTTGGG CATCACTACT GTCCTCACCA TGACAACCCA GAGCTCTGGG 
TCACGCGCAT CACTGCCAAA GGTCTCCTAT GTGAAGGCTA TAGACATCTG GATGGCGGTG 
TGTTTGCTGT TCGTCTTCGC TGCTCTTCTT GAATACGCCG GAGTAAACTT TGTCTCCAGA 
CAACAGAAAG AGTTTCTGCG CTTGAAACGA AGACAAAGAC GAACTCAAAA GGAGGAGGAT 
TTACAGGACG GACGTTTGCA TTTTTCCAGC TACAACACAA CGACCTGCGT AAAAGATGGC 
GCCGTGGTCA AAAACACACA GGTGAACCAA ATCCAGCAGC CGTCCATCCT GAAAAACACA 
GAAACAAACC GCAAGAAGTT TGTGGATCGA GCCAAACGAA TCGACACCAT ATCACGCGCC 
GCGTTCCCAC TGGCGTTCTT GATCTTCAAC GTTTTTTACT GGATCACGTA TAAGATCATC 
AGACACGAGA GCGCAAGAAA AGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

glra2 ( XM_021478777.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021334452.1 Danio rerio glycine receptor, alpha 2 (glra2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa143998
Clone ID Related Accession (Same CDS sequence)	XM_021478777.1
Accession Version	XM_021478777.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1347bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	glycine receptor subunit alpha-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTCGCC CTTCGGTCAA ACTCCTCACA ACTTTACTCG CGTGTCTGAT GGAAATGCTC 
AACTTCAGGG TATCGTCAGG GAAGGATCCG GATCTTCTGT CCTCCTCCTC CTCCAGCATG 
TCTCCCTCTG ACTTCCTGGA TAAACTGATG GGCAGAACGT CCGGCTATGA CGCTCGCATT 
CGACCAAACT TCAAAGGTCC TCCTGTGAAC GTCACGTGTA ATATATTCAT CAACAGCTTC 
GGTTCAGTCA CAGAAACTAC TATGGACTAC AGGGTGAACA TTTTCCTCCG GCAGAAGTGG 
AACGACCCTC GACTGGCCTA CAGCGAGTAT CCGGACTCGT CTCTGGATCT GGACCCGTCC 
ATGCTGGACT CCATCTGGAA GCCTGACCTG TTCTTCGCCA ACGAGAAAGG AGCAAATTTT 
CACGACGTCA CGACAGACAA CAAACTCCTG AGGATCTTCA AGGACGGAAC CGTTCTGTAC 
AGCATCAGGC TGACGTTAAT ACTGTCCTGT CCGATGGATC TGAAGAATTT CCCTATGGAT 
GTTCAGACGT GTACCATGCA GCTGGAGAGC TTCGGATACA CCATGAATGA CCTGATTTTT 
GAGTGGCTGG ATAAAGGGCC CGTTCAGGTG GCTGATGGTC TGACTTTACC TCAGTTCCTC 
ATCCGGGACG AGAAAGACCT CGGATACTGT ACAAAACACT ATAACACAGG TAAGTTCACC 
TGTATCGAGG TGAAGTTCCA CCTGGAGCGG CAGATGGGTT ATTACCTGAT CCAGATGTAC 
ATCCCGAGTC TGCTGATCGT CATCCTGTCC TGGGTTTCCT TCTGGATCAA CATGGACGCG 
GCTCCGGCTC GAGTGGCTCT GGGCATCACT ACTGTCCTCA CCATGACAAC CCAGAGCTCT 
GGGTCACGCG CATCACTGCC AAAGGTCTCC TATGTGAAGG CTATAGACAT CTGGATGGCG 
GTGTGTTTGC TGTTCGTCTT CGCTGCTCTT CTTGAATACG CCGGAGTAAA CTTTGTCTCC 
AGACAACAGA AAGAGTTTCT GCGCTTGAAA CGAAGACAAA GACGAACTCA AAAGGAGGAT 
TTACAGGACG GACGTTTGCA TTTTTCCAGC TACAACACAA CGACCTGTGT AAAAGATGGC 
GCCGTGGTCA AAAACACACA GGTGAACCAA ATCCAGCAGC CGTCCATCCT GAAAAACACA 
GAAACAAACC GAAAGAAGTT TGTGGATCGA GCCAAACGAA TCGACACCAT ATCACGCGCC 
GCATTCCCAC TGGCGTTCTT AATCTTCAAT GTTTTTTACT GGATCACGTA TAAGATCATC 
AGACACGAGA GCGCAAGGAA AGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021334452.1
CDS	450..1796
Translation	MTRPSVKLLTTLLACLMEMLNFRVSSGKDPDLLSSSSSSMSPSDFLDKLMGRTSGYDARIRPNFKGPPVNVTCNIFINSFGSVTETTMDYRVNIFLRQKWNDPRLAYSEYPDSSLDLDPSMLDSIWKPDLFFANEKGANFHDVTTDNKLLRIFKDGTVLYSIRLTLILSCPMDLKNFPMDVQTCTMQLESFGYTMNDLIFEWLDKGPVQVADGLTLPQFLIRDEKDLGYCTKHYNTGKFTCIEVKFHLERQMGYYLIQMYIPSLLIVILSWVSFWINMDAAPARVALGITTVLTMTTQSSGSRASLPKVSYVKAIDIWMAVCLLFVFAALLEYAGVNFVSRQQKEFLRLKRRQRRTQKEDLQDGRLHFSSYNTTTCVKDGAVVKNTQVNQIQQPSILKNTETNRKKFVDRAKRIDTISRAAFPLAFLIFNVFYWITYKIIRHESARKD

Target ORF information:

RefSeq Version	XM_021478777.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio glycine receptor, alpha 2 (glra2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021478777.1

ORF Insert Sequence:

ATGACTCGCC CTTCGGTCAA ACTCCTCACA ACTTTACTCG CGTGTCTGAT GGAAATGCTC 
AACTTCAGGG TATCGTCAGG GAAGGATCCG GATCTTCTGT CCTCCTCCTC CTCCAGCATG 
TCTCCCTCTG ACTTCCTGGA TAAACTGATG GGCAGAACGT CCGGCTATGA CGCTCGCATT 
CGACCAAACT TCAAAGGTCC TCCTGTGAAC GTCACGTGTA ATATATTCAT CAACAGCTTC 
GGTTCAGTCA CAGAAACTAC TATGGACTAC AGGGTGAACA TTTTCCTCCG GCAGAAGTGG 
AACGACCCTC GACTGGCCTA CAGCGAGTAT CCGGACTCGT CTCTGGATCT GGACCCGTCC 
ATGCTGGACT CCATCTGGAA GCCTGACCTG TTCTTCGCCA ACGAGAAAGG AGCAAATTTT 
CACGACGTCA CGACAGACAA CAAACTCCTG AGGATCTTCA AGGACGGAAC CGTTCTGTAC 
AGCATCAGGC TGACGTTAAT ACTGTCCTGT CCGATGGATC TGAAGAATTT CCCTATGGAT 
GTTCAGACGT GTACCATGCA GCTGGAGAGC TTCGGATACA CCATGAATGA CCTGATTTTT 
GAGTGGCTGG ATAAAGGGCC CGTTCAGGTG GCTGATGGTC TGACTTTACC TCAGTTCCTC 
ATCCGGGACG AGAAAGACCT CGGATACTGT ACAAAACACT ATAACACAGG TAAGTTCACC 
TGTATCGAGG TGAAGTTCCA CCTGGAGCGG CAGATGGGTT ATTACCTGAT CCAGATGTAC 
ATCCCGAGTC TGCTGATCGT CATCCTGTCC TGGGTTTCCT TCTGGATCAA CATGGACGCG 
GCTCCGGCTC GAGTGGCTCT GGGCATCACT ACTGTCCTCA CCATGACAAC CCAGAGCTCT 
GGGTCACGCG CATCACTGCC AAAGGTCTCC TATGTGAAGG CTATAGACAT CTGGATGGCG 
GTGTGTTTGC TGTTCGTCTT CGCTGCTCTT CTTGAATACG CCGGAGTAAA CTTTGTCTCC 
AGACAACAGA AAGAGTTTCT GCGCTTGAAA CGAAGACAAA GACGAACTCA AAAGGAGGAT 
TTACAGGACG GACGTTTGCA TTTTTCCAGC TACAACACAA CGACCTGTGT AAAAGATGGC 
GCCGTGGTCA AAAACACACA GGTGAACCAA ATCCAGCAGC CGTCCATCCT GAAAAACACA 
GAAACAAACC GAAAGAAGTT TGTGGATCGA GCCAAACGAA TCGACACCAT ATCACGCGCC 
GCATTCCCAC TGGCGTTCTT AATCTTCAAT GTTTTTTACT GGATCACGTA TAAGATCATC 
AGACACGAGA GCGCAAGGAA AGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genetic and functional analyses demonstrate a role for abnormal glycinergic signaling in autism.
Molecular psychiatry21(7)936-45(2016 07)
Pilorge M,Fassier C,Le Corronc H,Potey A,Bai J,De Gois S,Delaby E,Assouline B,Guinchat V,Devillard F,Delorme R,Nygren G,R?stam M,Meier JC,Otani S,Cheval H,James VM,Topf M,Dear TN,Gillberg C,Leboyer M,Giros B,Gautron S,Hazan J,Harvey RJ,Legendre P,Betancur C

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

Defective escape behavior in DEAH-box RNA helicase mutants improved by restoring glycine receptor expression.
The Journal of neuroscience : the official journal of the Society for Neuroscience33(37)14638-44(2013 Sep)
Hirata H,Ogino K,Yamada K,Leacock S,Harvey RJ

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glra2 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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