spg11 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	spg11
Entrez Gene ID	793551
Full Name	spastic paraplegia 11
General protein information	Preferred Names spastic paraplegia 11 Names spatacsin
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001174135.1	NP_001167606.1	spatacsin isoform 2
NM_001194994.1	NP_001181923.1	spatacsin isoform 1

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Spg11	NP_001178500.1
Homo sapiens (human)	SPG11	NP_079413.3
Canis lupus familiaris (dog)	SPG11	XP_003640073.2
Bos taurus (cattle)	SPG11	XP_002691138.2
Mus musculus (house mouse)	Spg11	NP_663506.2
	LOC712221	XP_002808495.1
Gallus gallus (chicken)	SPG11	XP_003641879.2
Danio rerio (zebrafish)	spg11	NP_001181923.1

Gene Ontology
Bibliography

Related articles in PubMed

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Spatacsin and spastizin act in the same pathway required for proper spinal motor neuron axon outgrowth in zebrafish.
Martin E, Yanicostas C, Rastetter A, Alavi Naini SM, Maouedj A, Kabashi E, Rivaud-P?choux S, Brice A, Stevanin G, Soussi-Yanicostas N
Neurobiology of disease48(3)299-308(2012 Dec)

Novel SPG11 mutations in Asian kindreds and disruption of spatacsin function in the zebrafish.
Southgate L, Dafou D, Hoyle J, Li N, Kinning E, Critchley P, N?meth AH, Talbot K, Bindu PS, Sinha S, Taly AB, Raghavendra S, M?ller F, Maher ER, Trembath RC
Neurogenetics11(4)379-89(2010 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

analysis of SPG11 mutations in Asian kindreds and disruption of spatacsin function in the zebrafish
Title: Novel SPG11 mutations in Asian kindreds and disruption of spatacsin function in the zebrafish.

The following spg11 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the spg11 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa32783	NM_001174135.1 Latest version!	Danio rerio SPG11 vesicle trafficking associated, spatacsin (spg11), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
ODa32798	NM_001194994.1 Latest version!	Danio rerio SPG11 vesicle trafficking associated, spatacsin (spg11), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

spg11 ( NM_001174135.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001167606.1 Danio rerio SPG11 vesicle trafficking associated, spatacsin (spg11), transcript variant 2, mRNA.

Price & Availability↑

CloneID	ODa32783
Clone ID Related Accession (Same CDS sequence)	NM_001174135.1
Accession Version	NM_001174135.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3747bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-12
Organism	Danio rerio(zebrafish)
Product	spatacsin isoform 2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from FJ217178.1. ##Evidence-Data-START## Transcript exon combination :: FJ217178.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGCTGGAGG CTCTGAGCAG GAGTGATGCT GGAGCTCAGG CGGTGCTGGA GGTGGATTTG 
TTGGCGGATG GAGAGCTCTG TGTGGGGCTG AAAGACTGGC GGAGGGTGAA GCTGAGCGCG 
GGCCCGTCAC TGCTGTGCTG CGTGAACGCG CAGGGGGAAA CCAGTCTGAG GAACATCAGC 
GACAGCAGGA GAGCTGAGCA TACACTACAG CACACCTGTG TAGATTTCTT GTGGGAGGAT 
GTGAGTGTGA GCAGCGGTGC ACGTGTGAAG CCAAAGCTGC TGCTGCTAGA CTCTGAGTTT 
TCTCTGCATA TGTATGATGT GGATCAGGAT GATCAGCCGA CGCTGGTGTG TGAAACATCC 
TCTAATACAC TGCTTCAGAT CATCAAACAC ACACACGCCG ACATGTGTGC TGTGGCGTCT 
GTGAGGCTGT TGTGTTTCTC TGAGGGCCGC TGTATGTTGC TGGTGAACAC GTGTGTGTTA 
CTGCAGCTCA GACTAACGGA GGACACACTG GAGCCCATAT CCTGCTGCCG CCTCGGTGTG 
GAGTGTGTGG AGGAGTGTGT TGACTGCCGG CTGTGCAGAG GAGCTCTCTT CATCCTCCTT 
AACAGCGGAC TCTCATATGT GTTTGACAGC ACTGACGGGC AGCTGTTGGC GAAGGTGGAT 
TTTCCCTCAT ACTGCTGCAA TGCTGGTGTT TCTCCATCCA CCTCCTCCTT CTGTCTGCTC 
CAGATCTCTG CTGATCTCAG TACGGCTGTG AGCGTCACAC GGCAGAACCA GGCCTACGCC 
GTACACCTCG ACCACTACTT CAGTGTGTAT ACCGATCACC TGTACTGTCG TGCGGCCCCC 
ATTCGTCCAT TCCTCAGGCC TGTTGAATCC TGGGATCAGG ACAGTCTCTC CAGCTCCACC 
CACAGTCTAT CAGCACTAAA TAAACCCCTG CAGGCCGACC GGTCGTGGGA GTCTCGTCTG 
TCTGCGCTCT ACAATCAAAC TAAAGCTCCT GTTCGTCCCG TTCACATTTC ACCCTGGTAT 
AAAGACTTTC CTCACATCGA ATGCCGTCGC GCAGCCGCTA CAGCTAAACT CTGCCATTCC 
TCCGTGAAGA CAGGGGGCGC CGTTATGTCT TTTAATGTGC CAGATGGAGC CACACCAGCA 
GGACTCAACG TGTCAGAGTT TTCAGCACTA ATTACGTTCG TTTCTCCTGC TAACGCTAAT 
ACGGTTCTGG CCTACTGGGG TCTGGAGAAC GAAAGTGTGA CGTATCATCG TGCAGATGCT 
AATGCTATTC CTGTGCAGAG ATCAGCAGAG GAGAACCTGT GTCTATTACT AAAGACGTCG 
GGTCTGTCAG TGGTGCTGTT CTGCGTTTCT CAGGATGAGC TGCTCAACAG ACTGATGGTG 
TTCGGCAGCG CAGGAACAGT GGATTCACTC TGCCATCTGA ACGCATGGGG GCGCTGTTCA 
ATCCCTATAC ACGCCCTGCA GGCGGGTCTG AAGAACCATC AACTGGACAC AGTGGATTTC 
TTCCTGAAGA GTAAAGAGAA CATCTTGAGT TCTTCATCCG GCTTCAGTCC AGGAGATCCT 
CCTCCGTCAT CCTCCAACAC ACACACTCAT CTGCGGAGTA TCGAAGCTCT GTGTCCTGCC 
CTGGATCTCC TGCGCTCTGC GATTCGAGAC ACACACACTG AAGCTCAGAG TCAACAGTTC 
TGTGAGCAGC TCTTGAGCAT CACTATGAGC TTCCTGAACA CACAGACCAG AGTCCTGCTG 
AGCGGAGCGC ACGATCTGGA TGATAACCTG CAGCAGTGTG TGTGTGTTCT GGACGGCTTC 
ATCTCTGACC TGCGAGTGTT TCTGAAGAGA TTCCCCTGGG GTGTCGGAGG AGACGCTCGC 
ACACACACTG ATGCTCATAC ACACACTGCA GCGTCCGGCC GAGAGGAGGA GCACCAGTGG 
ATCTCGCTGT CTGATGAGGA GGTTGTTCAT CGCGCTGTCC TCAGCAGACG CATCTCTCAT 
GCTCAGGCTT TCCTGCGCCG CCGCGGCTCT GCTGAACACA CACTGCAGGA CATCAGGAGG 
ACTGGACTGA AACACACACA CACCTGCCTG ACCCTCCGAG ACCTGCAGCA GGCCACAACA 
CTGCTCACAC ACATGGGGTT TAATGTAAAG GAGCAGCTGC ACAACATCTG TGTTTACACA 
GCCGATCGGG ATCTAAGGGC CTTCATGGTG GAGGAGCTGC AGAAGCAGCA TCATCTCTCC 
GCTGACGAGC TGAAACAGGT GAACTTCATC CACACCATTG AGAGTCTGTG CTCCAAACCA 
GCCAACAGGA GCTCCAGACG ACTGGACGCC AACAGAGTGC TGCAGGTGAG CCGCTGTGAC 
CCTCAGAGTC GCCGTCTGCT GGAGAAGCTC CTCCAGGACT CAGACTCGTC CTCCACGTCT 
CTGCTGGATC CACTGCGTCT GCACTGGGTC AAACACTGGG ACAGAGACGC GCAGACGCAC 
ATCCTGCTGT CCCGCACACA CACACACACT CAGTGTTCAG GGGACGCGGC GGTGCTGTGG 
GCGTATCTGA CATCTCTGCA TGACTCGGTC CGCTGCTCTG CATGGCTGTC ATCATCATCT 
GTGTGTGACC GAGAGAGCAG CGCTGCCCCC TGCTGGCCAC AGCTCACAGC ACACGTCCTG 
GACACCTGCA CCCGCTGTGG AGAACACCTG CACAACATCA TCCTGGACCA GCTCGCACGA 
CAGGGACAGT TTGTGCCGGC CGAGATGCAG GACAGCATCG GCAGCGTCCC GCTGTCAGAC 
CTCAGCTGCA TCGTGGAGCT GCTGATTCTG GCTCATGACT GCTTCAGTGT CACCTGTAAT 
CTGGAAGGGA TTGTTCGTGT TCTTCAGGCC TGCAGACACC TGAGCCACAC ACACCTGGCG 
CACGGAGACG CTCACGGCTT GCTGGTGCGT CTGCTCACAG GTATCGGCAG GTATAATGAC 
ATGACGTACG TCTTCGATCT GCTGCACCAG AACCACCGAT TCGAGATGCT GCTGCGCAAG 
AAAGTGGAGT CGAATGTGCG TCTGAAGACG GCACTGCTGG ATTATATCAA GCGTTGTCTG 
CCTGGCGACA GCGAGAAGCA CAACATGGTG GCGCTGTGTT TCAGCATGTG CCGAGAGATC 
GGAGAAAACC ACGAGGCGGC GGCCAGAACA CAACTCAAAC TCATAGAGTC TCAGCCCTGG 
GTGATCACAG CAGAGCTGAA GAAGGCGTTA GTGAAGGTGC AGACGCTCCT CAAAGACGCA 
GCTGAGAGCT ACTCAAAGGA CTCGTGTGTC CGTCAGGCTG TGCGCTGTGT GAAGCTGGCG 
AAGCTGGTGA CTCTGCAGCT GCACTTCCTG AATCAGGGCC TGGAGCTGCG CATCATCAAC 
CTGCAGCACA CAGACCTGCA GAACACCAGC AACACACTGC CACACTGCTA CCAGGTGTTC 
GTGTTAGCAG AGGCGTATGA CTTCAGTCCA GACTGGGCCG AGGTTCTCTA TCAGAAGGTC 
ATCATGAGAG GAGACTTCAG CTACCTGGAG GAGTTCAAGA GGCACAGGCC GCTAGCTGCT 
AGTCTCTTCC AGGACATCTC CAACAAGGTG TGTCATCAGA AGCCCCCCGC GAGCTGCAGT 
CAGCACCTGA AGAAGCTGCT GTCGCACTGT GAGGACGTCT ACACGCATTA TAAACTGGCC 
TACGAGCACC AGTTCCTGGA CGTGGCCAAC ATGCTGCTGC AGGACTCCAA GACCAACAGC 
TTCCTGAAGG ACCGCCTCCG CACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001167606.1
CDS	247..3993
Translation	MLEALSRSDAGAQAVLEVDLLADGELCVGLKDWRRVKLSAGPSLLCCVNAQGETSLRNISDSRRAEHTLQHTCVDFLWEDVSVSSGARVKPKLLLLDSEFSLHMYDVDQDDQPTLVCETSSNTLLQIIKHTHADMCAVASVRLLCFSEGRCMLLVNTCVLLQLRLTEDTLEPISCCRLGVECVEECVDCRLCRGALFILLNSGLSYVFDSTDGQLLAKVDFPSYCCNAGVSPSTSSFCLLQISADLSTAVSVTRQNQAYAVHLDHYFSVYTDHLYCRAAPIRPFLRPVESWDQDSLSSSTHSLSALNKPLQADRSWESRLSALYNQTKAPVRPVHISPWYKDFPHIECRRAAATAKLCHSSVKTGGAVMSFNVPDGATPAGLNVSEFSALITFVSPANANTVLAYWGLENESVTYHRADANAIPVQRSAEENLCLLLKTSGLSVVLFCVSQDELLNRLMVFGSAGTVDSLCHLNAWGRCSIPIHALQAGLKNHQLDTVDFFLKSKENILSSSSGFSPGDPPPSSSNTHTHLRSIEALCPALDLLRSAIRDTHTEAQSQQFCEQLLSITMSFLNTQTRVLLSGAHDLDDNLQQCVCVLDGFISDLRVFLKRFPWGVGGDARTHTDAHTHTAASGREEEHQWISLSDEEVVHRAVLSRRISHAQAFLRRRGSAEHTLQDIRRTGLKHTHTCLTLRDLQQATTLLTHMGFNVKEQLHNICVYTADRDLRAFMVEELQKQHHLSADELKQVNFIHTIESLCSKPANRSSRRLDANRVLQVSRCDPQSRRLLEKLLQDSDSSSTSLLDPLRLHWVKHWDRDAQTHILLSRTHTHTQCSGDAAVLWAYLTSLHDSVRCSAWLSSSSVCDRESSAAPCWPQLTAHVLDTCTRCGEHLHNIILDQLARQGQFVPAEMQDSIGSVPLSDLSCIVELLILAHDCFSVTCNLEGIVRVLQACRHLSHTHLAHGDAHGLLVRLLTGIGRYNDMTYVFDLLHQNHRFEMLLRKKVESNVRLKTALLDYIKRCLPGDSEKHNMVALCFSMCREIGENHEAAARTQLKLIESQPWVITAELKKALVKVQTLLKDAAESYSKDSCVRQAVRCVKLAKLVTLQLHFLNQGLELRIINLQHTDLQNTSNTLPHCYQVFVLAEAYDFSPDWAEVLYQKVIMRGDFSYLEEFKRHRPLAASLFQDISNKVCHQKPPASCSQHLKKLLSHCEDVYTHYKLAYEHQFLDVANMLLQDSKTNSFLKDRLRT

Target ORF information:

RefSeq Version	NM_001174135.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio SPG11 vesicle trafficking associated, spatacsin (spg11), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001174135.1

ORF Insert Sequence:

ATGCTGGAGG CTCTGAGCAG GAGTGATGCT GGAGCTCAGG CGGTGCTGGA GGTGGATTTG 
TTGGCGGATG GAGAGCTCTG TGTGGGGCTG AAAGACTGGC GGAGGGTGAA GCTGAGCGCG 
GGCCCGTCAC TGCTGTGCTG CGTGAACGCG CAGGGGGAAA CCAGTCTGAG GAACATCAGC 
GACAGCAGGA GAGCTGAGCA TACACTACAG CACACCTGTG TAGATTTCTT GTGGGAGGAT 
GTGAGTGTGA GCAGCGGTGC ACGTGTGAAG CCAAAGCTGC TGCTGCTAGA CTCTGAGTTT 
TCTCTGCATA TGTATGATGT GGATCAGGAT GATCAGCCGA CGCTGGTGTG TGAAACATCC 
TCTAATACAC TGCTTCAGAT CATCAAACAC ACACACGCCG ACATGTGTGC TGTGGCGTCT 
GTGAGGCTGT TGTGTTTCTC TGAGGGCCGC TGTATGTTGC TGGTGAACAC GTGTGTGTTA 
CTGCAGCTCA GACTAACGGA GGACACACTG GAGCCCATAT CCTGCTGCCG CCTCGGTGTG 
GAGTGTGTGG AGGAGTGTGT TGACTGCCGG CTGTGCAGAG GAGCTCTCTT CATCCTCCTT 
AACAGCGGAC TCTCATATGT GTTTGACAGC ACTGACGGGC AGCTGTTGGC GAAGGTGGAT 
TTTCCCTCAT ACTGCTGCAA TGCTGGTGTT TCTCCATCCA CCTCCTCCTT CTGTCTGCTC 
CAGATCTCTG CTGATCTCAG TACGGCTGTG AGCGTCACAC GGCAGAACCA GGCCTACGCC 
GTACACCTCG ACCACTACTT CAGTGTGTAT ACCGATCACC TGTACTGTCG TGCGGCCCCC 
ATTCGTCCAT TCCTCAGGCC TGTTGAATCC TGGGATCAGG ACAGTCTCTC CAGCTCCACC 
CACAGTCTAT CAGCACTAAA TAAACCCCTG CAGGCCGACC GGTCGTGGGA GTCTCGTCTG 
TCTGCGCTCT ACAATCAAAC TAAAGCTCCT GTTCGTCCCG TTCACATTTC ACCCTGGTAT 
AAAGACTTTC CTCACATCGA ATGCCGTCGC GCAGCCGCTA CAGCTAAACT CTGCCATTCC 
TCCGTGAAGA CAGGGGGCGC CGTTATGTCT TTTAATGTGC CAGATGGAGC CACACCAGCA 
GGACTCAACG TGTCAGAGTT TTCAGCACTA ATTACGTTCG TTTCTCCTGC TAACGCTAAT 
ACGGTTCTGG CCTACTGGGG TCTGGAGAAC GAAAGTGTGA CGTATCATCG TGCAGATGCT 
AATGCTATTC CTGTGCAGAG ATCAGCAGAG GAGAACCTGT GTCTATTACT AAAGACGTCG 
GGTCTGTCAG TGGTGCTGTT CTGCGTTTCT CAGGATGAGC TGCTCAACAG ACTGATGGTG 
TTCGGCAGCG CAGGAACAGT GGATTCACTC TGCCATCTGA ACGCATGGGG GCGCTGTTCA 
ATCCCTATAC ACGCCCTGCA GGCGGGTCTG AAGAACCATC AACTGGACAC AGTGGATTTC 
TTCCTGAAGA GTAAAGAGAA CATCTTGAGT TCTTCATCCG GCTTCAGTCC AGGAGATCCT 
CCTCCGTCAT CCTCCAACAC ACACACTCAT CTGCGGAGTA TCGAAGCTCT GTGTCCTGCC 
CTGGATCTCC TGCGCTCTGC GATTCGAGAC ACACACACTG AAGCTCAGAG TCAACAGTTC 
TGTGAGCAGC TCTTGAGCAT CACTATGAGC TTCCTGAACA CACAGACCAG AGTCCTGCTG 
AGCGGAGCGC ACGATCTGGA TGATAACCTG CAGCAGTGTG TGTGTGTTCT GGACGGCTTC 
ATCTCTGACC TGCGAGTGTT TCTGAAGAGA TTCCCCTGGG GTGTCGGAGG AGACGCTCGC 
ACACACACTG ATGCTCATAC ACACACTGCA GCGTCCGGCC GAGAGGAGGA GCACCAGTGG 
ATCTCGCTGT CTGATGAGGA GGTTGTTCAT CGCGCTGTCC TCAGCAGACG CATCTCTCAT 
GCTCAGGCTT TCCTGCGCCG CCGCGGCTCT GCTGAACACA CACTGCAGGA CATCAGGAGG 
ACTGGACTGA AACACACACA CACCTGCCTG ACCCTCCGAG ACCTGCAGCA GGCCACAACA 
CTGCTCACAC ACATGGGGTT TAATGTAAAG GAGCAGCTGC ACAACATCTG TGTTTACACA 
GCCGATCGGG ATCTAAGGGC CTTCATGGTG GAGGAGCTGC AGAAGCAGCA TCATCTCTCC 
GCTGACGAGC TGAAACAGGT GAACTTCATC CACACCATTG AGAGTCTGTG CTCCAAACCA 
GCCAACAGGA GCTCCAGACG ACTGGACGCC AACAGAGTGC TGCAGGTGAG CCGCTGTGAC 
CCTCAGAGTC GCCGTCTGCT GGAGAAGCTC CTCCAGGACT CAGACTCGTC CTCCACGTCT 
CTGCTGGATC CACTGCGTCT GCACTGGGTC AAACACTGGG ACAGAGACGC GCAGACGCAC 
ATCCTGCTGT CCCGCACACA CACACACACT CAGTGTTCAG GGGACGCGGC GGTGCTGTGG 
GCGTATCTGA CATCTCTGCA TGACTCGGTC CGCTGCTCTG CATGGCTGTC ATCATCATCT 
GTGTGTGACC GAGAGAGCAG CGCTGCCCCC TGCTGGCCAC AGCTCACAGC ACACGTCCTG 
GACACCTGCA CCCGCTGTGG AGAACACCTG CACAACATCA TCCTGGACCA GCTCGCACGA 
CAGGGACAGT TTGTGCCGGC CGAGATGCAG GACAGCATCG GCAGCGTCCC GCTGTCAGAC 
CTCAGCTGCA TCGTGGAGCT GCTGATTCTG GCTCATGACT GCTTCAGTGT CACCTGTAAT 
CTGGAAGGGA TTGTTCGTGT TCTTCAGGCC TGCAGACACC TGAGCCACAC ACACCTGGCG 
CACGGAGACG CTCACGGCTT GCTGGTGCGT CTGCTCACAG GTATCGGCAG GTATAATGAC 
ATGACGTACG TCTTCGATCT GCTGCACCAG AACCACCGAT TCGAGATGCT GCTGCGCAAG 
AAAGTGGAGT CGAATGTGCG TCTGAAGACG GCACTGCTGG ATTATATCAA GCGTTGTCTG 
CCTGGCGACA GCGAGAAGCA CAACATGGTG GCGCTGTGTT TCAGCATGTG CCGAGAGATC 
GGAGAAAACC ACGAGGCGGC GGCCAGAACA CAACTCAAAC TCATAGAGTC TCAGCCCTGG 
GTGATCACAG CAGAGCTGAA GAAGGCGTTA GTGAAGGTGC AGACGCTCCT CAAAGACGCA 
GCTGAGAGCT ACTCAAAGGA CTCGTGTGTC CGTCAGGCTG TGCGCTGTGT GAAGCTGGCG 
AAGCTGGTGA CTCTGCAGCT GCACTTCCTG AATCAGGGCC TGGAGCTGCG CATCATCAAC 
CTGCAGCACA CAGACCTGCA GAACACCAGC AACACACTGC CACACTGCTA CCAGGTGTTC 
GTGTTAGCAG AGGCGTATGA CTTCAGTCCA GACTGGGCCG AGGTTCTCTA TCAGAAGGTC 
ATCATGAGAG GAGACTTCAG CTACCTGGAG GAGTTCAAGA GGCACAGGCC GCTAGCTGCT 
AGTCTCTTCC AGGACATCTC CAACAAGGTG TGTCATCAGA AGCCCCCCGC GAGCTGCAGT 
CAGCACCTGA AGAAGCTGCT GTCGCACTGT GAGGACGTCT ACACGCATTA TAAACTGGCC 
TACGAGCACC AGTTCCTGGA CGTGGCCAAC ATGCTGCTGC AGGACTCCAA GACCAACAGC 
TTCCTGAAGG ACCGCCTCCG CACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

spg11 ( NM_001194994.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001181923.1 Danio rerio SPG11 vesicle trafficking associated, spatacsin (spg11), transcript variant 1, mRNA.

Price & Availability↑

CloneID	ODa32798
Clone ID Related Accession (Same CDS sequence)	NM_001194994.1
Accession Version	NM_001194994.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7140bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-12
Organism	Danio rerio(zebrafish)
Product	spatacsin isoform 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from FJ217177.1. ##Evidence-Data-START## Transcript exon combination :: FJ217177.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGCTGGAGG CTCTGAGCAG GAGTGATGCT GGAGCTCAGG CGGTGCTGGA GGTGGATTTG 
TTGGCGGATG GAGAGCTCTG TGTGGGGCTG AAAGACTGGC GGAGGGTGAA GCTGAGCGCG 
GGCCCGTCAC TGCTGTGCTG CGTGAACGCG CAGGGGGAAA CCAGTCTGAG GAACATCAGC 
GACAGCAGGA GAGCTGAGCA TACACTACAG CACACCTGTG TAGATTTCTT GTGGGAGGAT 
GTGAGTGTGA GCAGCGGTGC ACGTGTGAAG CCAAAGCTGC TGCTGCTAGA CTCTGAGTTT 
TCTCTGCATA TGTATGATGT GGATCAGGAT GATCAGCCGA CGCTGGTGTG TGAAACATCC 
TCTAATACAC TGCTTCAGAT CATCAAACAC ACACACGCCG ACATGTGTGC TGTGGCGTCT 
GTGAGGCTGT TGTGTTTCTC TGAGGGCCGC TGTATGTTGC TGGTGAACAC GTGTGTGTTA 
CTGCAGCTCA GACTAACGGA GGACACACTG GAGCCCATAT CCTGCTGCCG CCTCGGTGTG 
GAGTGTGTGG AGGAGTGTGT TGACTGCCGG CTGTGCAGAG GAGCTCTCTT CATCCTCCTT 
AACAGCGGAC TCTCATATGT GTTTGACAGC ACTGACGGGC AGCTGTTGGC GAAGGTGGAT 
TTTCCCTCAT ACTGCTGCAA TGCTGGTGTT TCTCCATCCA CCTCCTCCTT CTGTCTGCTC 
CAGATCTCTG CTGATCTCAG TACGGCTGTG AGCGTCACAC GGCAGAACCA GGCCTACGCC 
GTACACCTCG ACCACTACTT CAGTGTGTAT ACCGATCACC TGTACTGTCG TGCGGCCCCC 
ATTCGTCCAT TCCTCAGGCC TGTTGAATCC TGGGATCAGG ACAGTCTCTC CAGCTCCACC 
CACAGTCTAT CAGCACTAAA TAAACCCCTG CAGGCCGACC GGTCGTGGGA GTCTCGTCTG 
TCTGCGCTCT ACAATCAAAC TAAAGCTCCT GTTCGTCCCG TTCACATTTC ACCCTGGTAT 
AAAGACTTTC CTCACATCGA ATGCCGTCGC GCAGCCGCTA CAGCTAAACT CTGCCATTCC 
TCCGTGAAGA CAGGGGGCGC CGTTATGTCT TTTAATGTGC CAGATGGAGC CACACCAGCA 
GGACTCAACG TGTCAGAGTT TTCAGCACTA ATTACGTTCG TTTCTCCTGC TAACGCTAAT 
ACGGTTCTGG CCTACTGGGG TCTGGAGAAC GAAAGTGTGA CGTATCATCG TGCAGATGCT 
AATGCTATTC CTGTGCAGAG ATCAGCAGAG GAGAACCTGT GTCTATTACT AAAGACGTCG 
GGTCTGTCAG TGGTGCTGTT CTGCGTTTCT CAGGATGAGC TGCTCAACAG ACTGATGGTG 
TTCGGCAGCG CAGGAACAGT GGATTCACTC TGCCATCTGA ACGCATGGGG GCGCTGTTCA 
ATCCCTATAC ACGCCCTGCA GGCGGGTCTG AAGAACCATC AACTGGACAC AGTGGATTTC 
TTCCTGAAGA GTAAAGAGAA CATCTTGAGT TCTTCATCCG GCTTCAGTCC AGGAGATCCT 
CCTCCGTCAT CCTCCAACAC ACACACTCAT CTGCGGAGTA TCGAAGCTCT GTGTCCTGCC 
CTGGATCTCC TGCGCTCTGC GATTCGAGAC ACACACACTG AAGCTCAGAG TCAACAGTTC 
TGTGAGCAGC TCTTGAGCAT CACTATGAGC TTCCTGAACA CACAGACCAG AGTCCTGCTG 
AGCGGAGCGC ACGATCTGGA TGATAACCTG CAGCAGTGTG TGTGTGTTCT GGACGGCTTC 
ATCTCTGACC TGCGAGTGTT TCTGAAGAGA TTCCCCTGGG GTGTCGGAGG AGACGCTCGC 
ACACACACTG ATGCTCATAC ACACACTGCA GCGTCCGGCC GAGAGGAGGA GCACCAGTGG 
ATCTCGCTGT CTGATGAGGA GGTTGTTCAT CGCGCTGTCC TCAGCAGACG CATCTCTCAT 
GCTCAGGCTT TCCTGCGCCG CCGCGGCTCT GCTGAACACA CACTGCAGGA CATCAGGAGG 
ACTGGACTGA AACACACACA CACCTGCCTG ACCCTCCGAG ACCTGCAGCA GGCCACAACA 
CTGCTCACAC ACATGGGGTT TAATGTAAAG GAGCAGCTGC ACAACATCTG TGTTTACACA 
GCCGATCGGG ATCTAAGGGC CTTCATGGTG GAGGAGCTGC AGAAGCAGCA TCATCTCTCC 
GCTGACGAGC TGAAACAGGT GAACTTCATC CACACCATTG AGAGTCTGTG CTCCAAACCA 
GCCAACAGGA GCTCCAGACG ACTGGACGCC AACAGAGTGC TGCAGGTGAG CCGCTGTGAC 
CCTCAGAGTC GCCGTCTGCT GGAGAAGCTC CTCCAGGACT CAGACTCGTC CTCCACGTCT 
CTGCTGGATC CACTGCGTCT GCACTGGGTC AAACACTGGG ACAGAGACGC GCAGACGCAC 
ATCCTGCTGT CCCGCACACA CACACACACT CAGTGTTCAG GGGACGCGGC GGTGCTGTGG 
GCGTATCTGA CATCTCTGCA TGACTCGGTC CGCTGCTCTG CATGGCTGTC ATCATCATCT 
GTGTGTGACC GAGAGAGCAG CGCTGCCCCC TGCTGGCCAC AGCTCACAGC ACACGTCCTG 
GACACCTGCA CCCGCTGTGG AGAACACCTG CACAACATCA TCCTGGACCA GCTCGCACGA 
CAGGGACAGT TTGTGCCGGC CGAGATGCAG GACTTCCAGC AGCTGCTGTG GAGACTGGCT 
CAGGCTGGAG GAGTGATGCA TGATGGGAAG GAGCCTTCGC TTCCTCAGGC TGGAGGAGTG 
ATGCATGATG GGAAGGAGCT CTGCTGTTCA CAGGAGCAGG AGTTTCACCA GCGCTTCATC 
CTGCACTGTC TGGAGAACAA CCTGCAGTTC CTGCTCTACA CCTACCTGGA GCACTACGGG 
CTGAGCCCAG AGAACTGTCC TCTCCTGAGC GACAGCAGTG TGTGTGAGAG TTTCCCCTGC 
TTCCAGATGA TGCTGAAGCT GCAGAAGATC ACCACACACC TCACAGAGCC GGAGTGTGTA 
TTTGAGGCGA GTCTGGCGGC TGCGCAGGTT CTTCTTCTTC CCGGCAGTCA GGCGAGCATC 
AGCAGTGTGT TACTGGAGGG ACACAGCCTG CTCACACTCG CCACAGCCAT GTTCACAGCC 
GGCGGCATAC AGCAGGCGGT CAGTACAGTG GACCCTCAGC TCCTCAGGAT GGCTCTCCGT 
CAGTATCCTC AACTGAAGAC AGCACTGTTC CCTCAGAGCG GCCCTCGCGG AGATGTCTCG 
CTCTACCACC TGCTGCAGTC TCTACATCCC CTGGACTCCT CCAGACTCTT CGGATGGCAG 
TCCGCCAACA GTGTGTCTGC CAGCAGCGAA GCGTCAAGTG AGCCTCCTCA TTTCTCCAGC 
TCTCACCTCG TCAGTAATTT CGCCATCGTG GAGAATCTGG ATTTCCTGTA CTTCCTGCGT 
CACGGACGGC CGGCCTTCGC TTACGCTGTT TACCTCACAC AACACCTGAG AGACTGCAGT 
AACACTAAAC TACAGTTGTC TATGGCGGTG GATCAGTCGT ACTCGTTGGC TCTGTTGAAC 
TTCAGCAGTG CGAGTGTGTC GTCGGCGTGT GTGTGCTTTT GTGAGCTCAT GGGTGTGTGT 
AGCCTCAAAC TGAGGACGGA TCTGCGCACG CTGAACCTCA TTCACAAGCA CTGGAGTCAG 
AGCCGTGAGG ACGGAGACGC AGATTCACTG CGACACACTC TGGTGGAGAA GGCCAGTCAG 
CTGATTAGCG CTGAGCATCG CGCGGCTCAG GATCTGCTCC TCCATCTGGA AGCGGCCATC 
AGAGACGCTC TGGAGAAGAA GACCATCAGT CGCTGGTCGT TCGAGGCAGC GCAGGAATGG 
GCACTTCCTG TCCAGTTCTG TCGACTCCAC GCTCTTCCTC TAAGCTCCGC CTACCCGCTG 
GACTGCGCTC ATGATGGACA GTGGCTGCAC TTTCTGCTGT TCGTTCAGAC GCACCAGTAC 
CCGCCCCAAC AGGTCAGGTT TCTATCTGAA AGCTTCAGTC CGGCTCTGCA GTCTCACATT 
TCTCTGGCCT TCCAGGATCT GCGTTCGCCA ATAGAGGATC AGGGAGTCTC AGTGACTCCG 
CCCTCTCCAG ACGAGAGCTC TGATTGGCCC ACAGAGCTGT TCCAGGTGCT ACTGCAGAGT 
CAGGAGAAGC CCCACCCCTG GAGGATCCTG CTGTCAGAGG CAGTGCGGCG CCGCTGTGCT 
CCTCTGGCCG TCCTCGCTGC CTCACACCAG GGCTCAGAGC TGCTGCAGTG CCTCTGTGTG 
TGGATCTTGA CCTCGGTGGA TGATGATGAT GTCACTCAGG AAGCTGCCGC CCACATCAAC 
GAGAGCCCCG CCCACCACGT GTGGAACCTG CATGACCTGT CCATCCTCTG GAAGACGCTG 
CTGAGCCGCA GCAAGATTAG GCCTCTGATT CGCGGATTCC AGCTCTTTCA GAGGGAGTCT 
CCGCTGATTG CCATGCTTCA GATGTACGAG TTTTGCTTCG ACTACAAAGA CTATCAGAGA 
GCCAAAGACA AACTGCTGGA CTTCCAGAAG AGTCTGCTTA ATTTGCGTAG CAGCAGTGTG 
TTGTCCAGCT CTGATGTTCT GCCAGTGCAG TGGGTGGAGT CTCAGGCTTC AGTGCTGCTC 
CTGACGCTCC TCAAGCAGAG CCGGACGCAG TATGAGCTCC GCCGGCTGCT GCAGCTGCTG 
CTGGATGTGG AGAAACTGCT CAAGTCCAAC GGTCCAGATT TCCGGCTGCT CAGTGAACTC 
AGTCAGCTGC TGTGTGAGAC GCCGGTGTGT GTGTCGTCTC GTCTGCTTGA CTGTTATTCC 
CCCAGTGTCC TGCAGGAGGA GTGTGTGAGG ATCATGGAGG AGCTGCAGGA CGCCGGACTC 
TACATGCAGG CGCGCAGAGT CGCAGAGCTG GCCGAGCTGC CCGCACACAC ACTCATCATC 
AACCAGCTTC TGCGGGATCT GAGCGCTCAG AAGCTGAAGC GTCAGTGGGA GCGTCAGGAG 
ATTCGATTGG CCTTCTGGAG GAGGTCACAT GATCAGCTGC AGACCGAACA CGTGCAGCCA 
GAGACCGCAT GCCACTTCTT CCTCTCACAG GCCGAGGTGG CGCTGCAGTC AGACTCAGAG 
CTGGTGAATC TCCAGGAGCG CTGTGTTCTG CTGGGTTTAG CAGGACTCTG GTTGTCTCGC 
TGTGATCCTC CACCATCCAC TCCAATTTTA GCGGATCTGG AGAAGCGTCA ATGGGAATGT 
CGGATCCAGC AGCGACTCCT ACTGGCGGAT CTGGAGCGTC AGAGTCTGTT CGCTCCGTCC 
GCACTCTCCG AAGACTCTCC GGAAAATCTC CTGCGCGATT TTTCCTTCTC CAGAATGCCG 
GCTCTTAATG ACGATGCTAA TCTGAGTCTG GACGGTCTGC CGGCGGATTC AAATTCCTGT 
ATTCTGCCTG ATGACCAGCA GAGGGCGCTG GCGAGTCTGA TCGCGCAGCT ACTGAACGAC 
GGCAGTGTGA ACGAGGCTAG CCGTGTGTGT CGCTACTTCA GGTTTTTTCA CAGAGATGTG 
TGGCTGGTGT TGCGGTGTCG CGGGTTAGCA TGCGGCGAAC TGCAGCCTGA ACTTCACAGT 
CCTGATGGAG AGAGCCGCCG CAGTCTGCCG TCATCTCCCA GTATGAGCTC CCTGTCGTCG 
TTTGTGGTGG TGTCGTCTCC TGATGATCAG CTCCAGCTTC AGCTCCAGCT GCTGGTGGAT 
CAGTGCAGCC ACGGCAAGAG TTACTGCAAA CAAGTGCTGA GCCTCTACCA GCTCTCCAAG 
GAGCTGCAGT GCTCGTTCTC TGAGCTGTGG TCCTCTGAGC CGGAGTGTGT CCTGCAGAAG 
GTGCTTCTGT CTCGTCAGTC TGAGCGCTGT AAGAAAGCAG AGGCCTTCAT CAGTGCTCAG 
AGTCTGCAGC CGCACACCGT CGCCAACCTC ATCTCCACTG CAGTTCTGGA GGGACTGACC 
GCACACACAC GAGACGCTGA CACTAGTGAG CGTCTGATTT TCAGTGCGTC GGACAGCAGA 
GAGTCGTTCC TCCAGCTCGC CAAACTGTGT GGAGACGCCA ATCTGGTGGG AAACAAACTG 
CTGGACAGCA TCGGCAGCGT CCCGCTGTCA GACCTCAGCT GCATCGTGGA GCTGCTGATT 
CTGGCTCATG ACTGCTTCAG TGTCACCTGT AATCTGGAAG GGATTGTTCG TGTTCTTCAG 
GCCTGCAGAC ACCTGAGCCA CACACACCTG GCGCACGGAG ACGCTCACGG CTTGCTGGTG 
CGTCTGCTCA CAGGTATCGG CAGGTATAAT GACATGACGT ACGTCTTCGA TCTGCTGCAC 
CAGAACCACC GATTCGAGAT GCTGCTGCGC AAGAAAGTGG AGTCGAATGT GCGTCTGAAG 
ACGGCACTGC TGGATTATAT CAAGCGTTGT CTGCCTGGCG ACAGCGAGAA GCACAACATG 
GTGGCGCTGT GTTTCAGCAT GTGCCGAGAG ATCGGAGAAA ACCACGAGGC GGCGGCCAGA 
ACACAACTCA AACTCATAGA GTCTCAGCCC TGGGTGATCA CAGCAGAGCT GAAGAAGGCG 
TTAGTGAAGG TGCAGACGCT CCTCAAAGAC GCAGCTGAGA GCTACTCAAA GGACTCGTGT 
GTCCGTCAGG CTGTGCGCTG TGTGAAGCTG GCGAAGCTGG TGACTCTGCA GCTGCACTTC 
CTGAATCAGG GCCTGGAGCT GCGCATCATC AACCTGCAGC ACACAGACCT GCAGAACACC 
AGCAACACAC TGCCACACTG CTACCAGGTG TTCGTGTTAG CAGAGGCGTA TGACTTCAGT 
CCAGACTGGG CCGAGGTTCT CTATCAGAAG GTCATCATGA GAGGAGACTT CAGCTACCTG 
GAGGAGTTCA AGAGGCACAG GCCGCTAGCT GCTAGTCTCT TCCAGGACAT CTCCAACAAG 
GTGTGTCATC AGAAGCCCCC CGCGAGCTGC AGTCAGCACC TGAAGAAGCT GCTGTCGCAC 
TGTGAGGACG TCTACACGCA TTATAAACTG GCCTACGAGC ACCAGTTCCT GGACGTGGCC 
AACATGCTGC TGCAGGACTC CAAGACCAAC AGCTTCCTGA AGGACCGCCT CCGCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001181923.1
CDS	247..7386
Translation	MLEALSRSDAGAQAVLEVDLLADGELCVGLKDWRRVKLSAGPSLLCCVNAQGETSLRNISDSRRAEHTLQHTCVDFLWEDVSVSSGARVKPKLLLLDSEFSLHMYDVDQDDQPTLVCETSSNTLLQIIKHTHADMCAVASVRLLCFSEGRCMLLVNTCVLLQLRLTEDTLEPISCCRLGVECVEECVDCRLCRGALFILLNSGLSYVFDSTDGQLLAKVDFPSYCCNAGVSPSTSSFCLLQISADLSTAVSVTRQNQAYAVHLDHYFSVYTDHLYCRAAPIRPFLRPVESWDQDSLSSSTHSLSALNKPLQADRSWESRLSALYNQTKAPVRPVHISPWYKDFPHIECRRAAATAKLCHSSVKTGGAVMSFNVPDGATPAGLNVSEFSALITFVSPANANTVLAYWGLENESVTYHRADANAIPVQRSAEENLCLLLKTSGLSVVLFCVSQDELLNRLMVFGSAGTVDSLCHLNAWGRCSIPIHALQAGLKNHQLDTVDFFLKSKENILSSSSGFSPGDPPPSSSNTHTHLRSIEALCPALDLLRSAIRDTHTEAQSQQFCEQLLSITMSFLNTQTRVLLSGAHDLDDNLQQCVCVLDGFISDLRVFLKRFPWGVGGDARTHTDAHTHTAASGREEEHQWISLSDEEVVHRAVLSRRISHAQAFLRRRGSAEHTLQDIRRTGLKHTHTCLTLRDLQQATTLLTHMGFNVKEQLHNICVYTADRDLRAFMVEELQKQHHLSADELKQVNFIHTIESLCSKPANRSSRRLDANRVLQVSRCDPQSRRLLEKLLQDSDSSSTSLLDPLRLHWVKHWDRDAQTHILLSRTHTHTQCSGDAAVLWAYLTSLHDSVRCSAWLSSSSVCDRESSAAPCWPQLTAHVLDTCTRCGEHLHNIILDQLARQGQFVPAEMQDFQQLLWRLAQAGGVMHDGKEPSLPQAGGVMHDGKELCCSQEQEFHQRFILHCLENNLQFLLYTYLEHYGLSPENCPLLSDSSVCESFPCFQMMLKLQKITTHLTEPECVFEASLAAAQVLLLPGSQASISSVLLEGHSLLTLATAMFTAGGIQQAVSTVDPQLLRMALRQYPQLKTALFPQSGPRGDVSLYHLLQSLHPLDSSRLFGWQSANSVSASSEASSEPPHFSSSHLVSNFAIVENLDFLYFLRHGRPAFAYAVYLTQHLRDCSNTKLQLSMAVDQSYSLALLNFSSASVSSACVCFCELMGVCSLKLRTDLRTLNLIHKHWSQSREDGDADSLRHTLVEKASQLISAEHRAAQDLLLHLEAAIRDALEKKTISRWSFEAAQEWALPVQFCRLHALPLSSAYPLDCAHDGQWLHFLLFVQTHQYPPQQVRFLSESFSPALQSHISLAFQDLRSPIEDQGVSVTPPSPDESSDWPTELFQVLLQSQEKPHPWRILLSEAVRRRCAPLAVLAASHQGSELLQCLCVWILTSVDDDDVTQEAAAHINESPAHHVWNLHDLSILWKTLLSRSKIRPLIRGFQLFQRESPLIAMLQMYEFCFDYKDYQRAKDKLLDFQKSLLNLRSSSVLSSSDVLPVQWVESQASVLLLTLLKQSRTQYELRRLLQLLLDVEKLLKSNGPDFRLLSELSQLLCETPVCVSSRLLDCYSPSVLQEECVRIMEELQDAGLYMQARRVAELAELPAHTLIINQLLRDLSAQKLKRQWERQEIRLAFWRRSHDQLQTEHVQPETACHFFLSQAEVALQSDSELVNLQERCVLLGLAGLWLSRCDPPPSTPILADLEKRQWECRIQQRLLLADLERQSLFAPSALSEDSPENLLRDFSFSRMPALNDDANLSLDGLPADSNSCILPDDQQRALASLIAQLLNDGSVNEASRVCRYFRFFHRDVWLVLRCRGLACGELQPELHSPDGESRRSLPSSPSMSSLSSFVVVSSPDDQLQLQLQLLVDQCSHGKSYCKQVLSLYQLSKELQCSFSELWSSEPECVLQKVLLSRQSERCKKAEAFISAQSLQPHTVANLISTAVLEGLTAHTRDADTSERLIFSASDSRESFLQLAKLCGDANLVGNKLLDSIGSVPLSDLSCIVELLILAHDCFSVTCNLEGIVRVLQACRHLSHTHLAHGDAHGLLVRLLTGIGRYNDMTYVFDLLHQNHRFEMLLRKKVESNVRLKTALLDYIKRCLPGDSEKHNMVALCFSMCREIGENHEAAARTQLKLIESQPWVITAELKKALVKVQTLLKDAAESYSKDSCVRQAVRCVKLAKLVTLQLHFLNQGLELRIINLQHTDLQNTSNTLPHCYQVFVLAEAYDFSPDWAEVLYQKVIMRGDFSYLEEFKRHRPLAASLFQDISNKVCHQKPPASCSQHLKKLLSHCEDVYTHYKLAYEHQFLDVANMLLQDSKTNSFLKDRLRT

Target ORF information:

RefSeq Version	NM_001194994.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio SPG11 vesicle trafficking associated, spatacsin (spg11), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001194994.1

ORF Insert Sequence:

ATGCTGGAGG CTCTGAGCAG GAGTGATGCT GGAGCTCAGG CGGTGCTGGA GGTGGATTTG 
TTGGCGGATG GAGAGCTCTG TGTGGGGCTG AAAGACTGGC GGAGGGTGAA GCTGAGCGCG 
GGCCCGTCAC TGCTGTGCTG CGTGAACGCG CAGGGGGAAA CCAGTCTGAG GAACATCAGC 
GACAGCAGGA GAGCTGAGCA TACACTACAG CACACCTGTG TAGATTTCTT GTGGGAGGAT 
GTGAGTGTGA GCAGCGGTGC ACGTGTGAAG CCAAAGCTGC TGCTGCTAGA CTCTGAGTTT 
TCTCTGCATA TGTATGATGT GGATCAGGAT GATCAGCCGA CGCTGGTGTG TGAAACATCC 
TCTAATACAC TGCTTCAGAT CATCAAACAC ACACACGCCG ACATGTGTGC TGTGGCGTCT 
GTGAGGCTGT TGTGTTTCTC TGAGGGCCGC TGTATGTTGC TGGTGAACAC GTGTGTGTTA 
CTGCAGCTCA GACTAACGGA GGACACACTG GAGCCCATAT CCTGCTGCCG CCTCGGTGTG 
GAGTGTGTGG AGGAGTGTGT TGACTGCCGG CTGTGCAGAG GAGCTCTCTT CATCCTCCTT 
AACAGCGGAC TCTCATATGT GTTTGACAGC ACTGACGGGC AGCTGTTGGC GAAGGTGGAT 
TTTCCCTCAT ACTGCTGCAA TGCTGGTGTT TCTCCATCCA CCTCCTCCTT CTGTCTGCTC 
CAGATCTCTG CTGATCTCAG TACGGCTGTG AGCGTCACAC GGCAGAACCA GGCCTACGCC 
GTACACCTCG ACCACTACTT CAGTGTGTAT ACCGATCACC TGTACTGTCG TGCGGCCCCC 
ATTCGTCCAT TCCTCAGGCC TGTTGAATCC TGGGATCAGG ACAGTCTCTC CAGCTCCACC 
CACAGTCTAT CAGCACTAAA TAAACCCCTG CAGGCCGACC GGTCGTGGGA GTCTCGTCTG 
TCTGCGCTCT ACAATCAAAC TAAAGCTCCT GTTCGTCCCG TTCACATTTC ACCCTGGTAT 
AAAGACTTTC CTCACATCGA ATGCCGTCGC GCAGCCGCTA CAGCTAAACT CTGCCATTCC 
TCCGTGAAGA CAGGGGGCGC CGTTATGTCT TTTAATGTGC CAGATGGAGC CACACCAGCA 
GGACTCAACG TGTCAGAGTT TTCAGCACTA ATTACGTTCG TTTCTCCTGC TAACGCTAAT 
ACGGTTCTGG CCTACTGGGG TCTGGAGAAC GAAAGTGTGA CGTATCATCG TGCAGATGCT 
AATGCTATTC CTGTGCAGAG ATCAGCAGAG GAGAACCTGT GTCTATTACT AAAGACGTCG 
GGTCTGTCAG TGGTGCTGTT CTGCGTTTCT CAGGATGAGC TGCTCAACAG ACTGATGGTG 
TTCGGCAGCG CAGGAACAGT GGATTCACTC TGCCATCTGA ACGCATGGGG GCGCTGTTCA 
ATCCCTATAC ACGCCCTGCA GGCGGGTCTG AAGAACCATC AACTGGACAC AGTGGATTTC 
TTCCTGAAGA GTAAAGAGAA CATCTTGAGT TCTTCATCCG GCTTCAGTCC AGGAGATCCT 
CCTCCGTCAT CCTCCAACAC ACACACTCAT CTGCGGAGTA TCGAAGCTCT GTGTCCTGCC 
CTGGATCTCC TGCGCTCTGC GATTCGAGAC ACACACACTG AAGCTCAGAG TCAACAGTTC 
TGTGAGCAGC TCTTGAGCAT CACTATGAGC TTCCTGAACA CACAGACCAG AGTCCTGCTG 
AGCGGAGCGC ACGATCTGGA TGATAACCTG CAGCAGTGTG TGTGTGTTCT GGACGGCTTC 
ATCTCTGACC TGCGAGTGTT TCTGAAGAGA TTCCCCTGGG GTGTCGGAGG AGACGCTCGC 
ACACACACTG ATGCTCATAC ACACACTGCA GCGTCCGGCC GAGAGGAGGA GCACCAGTGG 
ATCTCGCTGT CTGATGAGGA GGTTGTTCAT CGCGCTGTCC TCAGCAGACG CATCTCTCAT 
GCTCAGGCTT TCCTGCGCCG CCGCGGCTCT GCTGAACACA CACTGCAGGA CATCAGGAGG 
ACTGGACTGA AACACACACA CACCTGCCTG ACCCTCCGAG ACCTGCAGCA GGCCACAACA 
CTGCTCACAC ACATGGGGTT TAATGTAAAG GAGCAGCTGC ACAACATCTG TGTTTACACA 
GCCGATCGGG ATCTAAGGGC CTTCATGGTG GAGGAGCTGC AGAAGCAGCA TCATCTCTCC 
GCTGACGAGC TGAAACAGGT GAACTTCATC CACACCATTG AGAGTCTGTG CTCCAAACCA 
GCCAACAGGA GCTCCAGACG ACTGGACGCC AACAGAGTGC TGCAGGTGAG CCGCTGTGAC 
CCTCAGAGTC GCCGTCTGCT GGAGAAGCTC CTCCAGGACT CAGACTCGTC CTCCACGTCT 
CTGCTGGATC CACTGCGTCT GCACTGGGTC AAACACTGGG ACAGAGACGC GCAGACGCAC 
ATCCTGCTGT CCCGCACACA CACACACACT CAGTGTTCAG GGGACGCGGC GGTGCTGTGG 
GCGTATCTGA CATCTCTGCA TGACTCGGTC CGCTGCTCTG CATGGCTGTC ATCATCATCT 
GTGTGTGACC GAGAGAGCAG CGCTGCCCCC TGCTGGCCAC AGCTCACAGC ACACGTCCTG 
GACACCTGCA CCCGCTGTGG AGAACACCTG CACAACATCA TCCTGGACCA GCTCGCACGA 
CAGGGACAGT TTGTGCCGGC CGAGATGCAG GACTTCCAGC AGCTGCTGTG GAGACTGGCT 
CAGGCTGGAG GAGTGATGCA TGATGGGAAG GAGCCTTCGC TTCCTCAGGC TGGAGGAGTG 
ATGCATGATG GGAAGGAGCT CTGCTGTTCA CAGGAGCAGG AGTTTCACCA GCGCTTCATC 
CTGCACTGTC TGGAGAACAA CCTGCAGTTC CTGCTCTACA CCTACCTGGA GCACTACGGG 
CTGAGCCCAG AGAACTGTCC TCTCCTGAGC GACAGCAGTG TGTGTGAGAG TTTCCCCTGC 
TTCCAGATGA TGCTGAAGCT GCAGAAGATC ACCACACACC TCACAGAGCC GGAGTGTGTA 
TTTGAGGCGA GTCTGGCGGC TGCGCAGGTT CTTCTTCTTC CCGGCAGTCA GGCGAGCATC 
AGCAGTGTGT TACTGGAGGG ACACAGCCTG CTCACACTCG CCACAGCCAT GTTCACAGCC 
GGCGGCATAC AGCAGGCGGT CAGTACAGTG GACCCTCAGC TCCTCAGGAT GGCTCTCCGT 
CAGTATCCTC AACTGAAGAC AGCACTGTTC CCTCAGAGCG GCCCTCGCGG AGATGTCTCG 
CTCTACCACC TGCTGCAGTC TCTACATCCC CTGGACTCCT CCAGACTCTT CGGATGGCAG 
TCCGCCAACA GTGTGTCTGC CAGCAGCGAA GCGTCAAGTG AGCCTCCTCA TTTCTCCAGC 
TCTCACCTCG TCAGTAATTT CGCCATCGTG GAGAATCTGG ATTTCCTGTA CTTCCTGCGT 
CACGGACGGC CGGCCTTCGC TTACGCTGTT TACCTCACAC AACACCTGAG AGACTGCAGT 
AACACTAAAC TACAGTTGTC TATGGCGGTG GATCAGTCGT ACTCGTTGGC TCTGTTGAAC 
TTCAGCAGTG CGAGTGTGTC GTCGGCGTGT GTGTGCTTTT GTGAGCTCAT GGGTGTGTGT 
AGCCTCAAAC TGAGGACGGA TCTGCGCACG CTGAACCTCA TTCACAAGCA CTGGAGTCAG 
AGCCGTGAGG ACGGAGACGC AGATTCACTG CGACACACTC TGGTGGAGAA GGCCAGTCAG 
CTGATTAGCG CTGAGCATCG CGCGGCTCAG GATCTGCTCC TCCATCTGGA AGCGGCCATC 
AGAGACGCTC TGGAGAAGAA GACCATCAGT CGCTGGTCGT TCGAGGCAGC GCAGGAATGG 
GCACTTCCTG TCCAGTTCTG TCGACTCCAC GCTCTTCCTC TAAGCTCCGC CTACCCGCTG 
GACTGCGCTC ATGATGGACA GTGGCTGCAC TTTCTGCTGT TCGTTCAGAC GCACCAGTAC 
CCGCCCCAAC AGGTCAGGTT TCTATCTGAA AGCTTCAGTC CGGCTCTGCA GTCTCACATT 
TCTCTGGCCT TCCAGGATCT GCGTTCGCCA ATAGAGGATC AGGGAGTCTC AGTGACTCCG 
CCCTCTCCAG ACGAGAGCTC TGATTGGCCC ACAGAGCTGT TCCAGGTGCT ACTGCAGAGT 
CAGGAGAAGC CCCACCCCTG GAGGATCCTG CTGTCAGAGG CAGTGCGGCG CCGCTGTGCT 
CCTCTGGCCG TCCTCGCTGC CTCACACCAG GGCTCAGAGC TGCTGCAGTG CCTCTGTGTG 
TGGATCTTGA CCTCGGTGGA TGATGATGAT GTCACTCAGG AAGCTGCCGC CCACATCAAC 
GAGAGCCCCG CCCACCACGT GTGGAACCTG CATGACCTGT CCATCCTCTG GAAGACGCTG 
CTGAGCCGCA GCAAGATTAG GCCTCTGATT CGCGGATTCC AGCTCTTTCA GAGGGAGTCT 
CCGCTGATTG CCATGCTTCA GATGTACGAG TTTTGCTTCG ACTACAAAGA CTATCAGAGA 
GCCAAAGACA AACTGCTGGA CTTCCAGAAG AGTCTGCTTA ATTTGCGTAG CAGCAGTGTG 
TTGTCCAGCT CTGATGTTCT GCCAGTGCAG TGGGTGGAGT CTCAGGCTTC AGTGCTGCTC 
CTGACGCTCC TCAAGCAGAG CCGGACGCAG TATGAGCTCC GCCGGCTGCT GCAGCTGCTG 
CTGGATGTGG AGAAACTGCT CAAGTCCAAC GGTCCAGATT TCCGGCTGCT CAGTGAACTC 
AGTCAGCTGC TGTGTGAGAC GCCGGTGTGT GTGTCGTCTC GTCTGCTTGA CTGTTATTCC 
CCCAGTGTCC TGCAGGAGGA GTGTGTGAGG ATCATGGAGG AGCTGCAGGA CGCCGGACTC 
TACATGCAGG CGCGCAGAGT CGCAGAGCTG GCCGAGCTGC CCGCACACAC ACTCATCATC 
AACCAGCTTC TGCGGGATCT GAGCGCTCAG AAGCTGAAGC GTCAGTGGGA GCGTCAGGAG 
ATTCGATTGG CCTTCTGGAG GAGGTCACAT GATCAGCTGC AGACCGAACA CGTGCAGCCA 
GAGACCGCAT GCCACTTCTT CCTCTCACAG GCCGAGGTGG CGCTGCAGTC AGACTCAGAG 
CTGGTGAATC TCCAGGAGCG CTGTGTTCTG CTGGGTTTAG CAGGACTCTG GTTGTCTCGC 
TGTGATCCTC CACCATCCAC TCCAATTTTA GCGGATCTGG AGAAGCGTCA ATGGGAATGT 
CGGATCCAGC AGCGACTCCT ACTGGCGGAT CTGGAGCGTC AGAGTCTGTT CGCTCCGTCC 
GCACTCTCCG AAGACTCTCC GGAAAATCTC CTGCGCGATT TTTCCTTCTC CAGAATGCCG 
GCTCTTAATG ACGATGCTAA TCTGAGTCTG GACGGTCTGC CGGCGGATTC AAATTCCTGT 
ATTCTGCCTG ATGACCAGCA GAGGGCGCTG GCGAGTCTGA TCGCGCAGCT ACTGAACGAC 
GGCAGTGTGA ACGAGGCTAG CCGTGTGTGT CGCTACTTCA GGTTTTTTCA CAGAGATGTG 
TGGCTGGTGT TGCGGTGTCG CGGGTTAGCA TGCGGCGAAC TGCAGCCTGA ACTTCACAGT 
CCTGATGGAG AGAGCCGCCG CAGTCTGCCG TCATCTCCCA GTATGAGCTC CCTGTCGTCG 
TTTGTGGTGG TGTCGTCTCC TGATGATCAG CTCCAGCTTC AGCTCCAGCT GCTGGTGGAT 
CAGTGCAGCC ACGGCAAGAG TTACTGCAAA CAAGTGCTGA GCCTCTACCA GCTCTCCAAG 
GAGCTGCAGT GCTCGTTCTC TGAGCTGTGG TCCTCTGAGC CGGAGTGTGT CCTGCAGAAG 
GTGCTTCTGT CTCGTCAGTC TGAGCGCTGT AAGAAAGCAG AGGCCTTCAT CAGTGCTCAG 
AGTCTGCAGC CGCACACCGT CGCCAACCTC ATCTCCACTG CAGTTCTGGA GGGACTGACC 
GCACACACAC GAGACGCTGA CACTAGTGAG CGTCTGATTT TCAGTGCGTC GGACAGCAGA 
GAGTCGTTCC TCCAGCTCGC CAAACTGTGT GGAGACGCCA ATCTGGTGGG AAACAAACTG 
CTGGACAGCA TCGGCAGCGT CCCGCTGTCA GACCTCAGCT GCATCGTGGA GCTGCTGATT 
CTGGCTCATG ACTGCTTCAG TGTCACCTGT AATCTGGAAG GGATTGTTCG TGTTCTTCAG 
GCCTGCAGAC ACCTGAGCCA CACACACCTG GCGCACGGAG ACGCTCACGG CTTGCTGGTG 
CGTCTGCTCA CAGGTATCGG CAGGTATAAT GACATGACGT ACGTCTTCGA TCTGCTGCAC 
CAGAACCACC GATTCGAGAT GCTGCTGCGC AAGAAAGTGG AGTCGAATGT GCGTCTGAAG 
ACGGCACTGC TGGATTATAT CAAGCGTTGT CTGCCTGGCG ACAGCGAGAA GCACAACATG 
GTGGCGCTGT GTTTCAGCAT GTGCCGAGAG ATCGGAGAAA ACCACGAGGC GGCGGCCAGA 
ACACAACTCA AACTCATAGA GTCTCAGCCC TGGGTGATCA CAGCAGAGCT GAAGAAGGCG 
TTAGTGAAGG TGCAGACGCT CCTCAAAGAC GCAGCTGAGA GCTACTCAAA GGACTCGTGT 
GTCCGTCAGG CTGTGCGCTG TGTGAAGCTG GCGAAGCTGG TGACTCTGCA GCTGCACTTC 
CTGAATCAGG GCCTGGAGCT GCGCATCATC AACCTGCAGC ACACAGACCT GCAGAACACC 
AGCAACACAC TGCCACACTG CTACCAGGTG TTCGTGTTAG CAGAGGCGTA TGACTTCAGT 
CCAGACTGGG CCGAGGTTCT CTATCAGAAG GTCATCATGA GAGGAGACTT CAGCTACCTG 
GAGGAGTTCA AGAGGCACAG GCCGCTAGCT GCTAGTCTCT TCCAGGACAT CTCCAACAAG 
GTGTGTCATC AGAAGCCCCC CGCGAGCTGC AGTCAGCACC TGAAGAAGCT GCTGTCGCAC 
TGTGAGGACG TCTACACGCA TTATAAACTG GCCTACGAGC ACCAGTTCCT GGACGTGGCC 
AACATGCTGC TGCAGGACTC CAAGACCAAC AGCTTCCTGA AGGACCGCCT CCGCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Spatacsin and spastizin act in the same pathway required for proper spinal motor neuron axon outgrowth in zebrafish.
Neurobiology of disease48(3)299-308(2012 Dec)
Martin E,Yanicostas C,Rastetter A,Alavi Naini SM,Maouedj A,Kabashi E,Rivaud-P?choux S,Brice A,Stevanin G,Soussi-Yanicostas N

Novel SPG11 mutations in Asian kindreds and disruption of spatacsin function in the zebrafish.
Neurogenetics11(4)379-89(2010 Oct)
Southgate L,Dafou D,Hoyle J,Li N,Kinning E,Critchley P,N?meth AH,Talbot K,Bindu PS,Sinha S,Taly AB,Raghavendra S,M?ller F,Maher ER,Trembath RC

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spg11 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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