SLC12A9 cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	SLC12A9
Entrez Gene ID	793341
Full Name	solute carrier family 12, member 9
Synonyms	fk87c04,im:7146124,si:ch211-284e13.1,wu:fk87c04
General protein information	Preferred Names solute carrier family 12, member 9 Names solute carrier family 12 member 9 solute carrier family 12 (potassium/chloride transporters), member 9
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005165365.4	XP_005165422.1	solute carrier family 12 member 9 isoform X1
NM_001128548.1	NP_001122020.1	solute carrier family 12 member 9
XM_017356172.1	XP_017211661.1	solute carrier family 12 member 9 isoform X1
XM_021476318.1	XP_021331993.1	solute carrier family 12 member 9 isoform X1
XM_005165365.3	XP_005165422.1	solute carrier family 12 member 9 isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	SLC12A9	NP_064631.2
	SLC12A9	XP_001105778.2
Caenorhabditis elegans (roundworm)	T04B8.5	NP_001022305.1
Saccharomyces cerevisiae (baker's yeast)	VHC1	NP_009794.3
Kluyveromyces lactis	KLLA0F10549g	XP_455560.1
Eremothecium gossypii	AGOS_AFL025C	NP_985523.1
Mus musculus (house mouse)	Slc12a9	NP_113583.2
Gallus gallus (chicken)	SLC12A9	XP_003641839.2
Danio rerio (zebrafish)	slc12a9	NP_001122020.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010249	XP_319440.3
Xenopus tropicalis (tropical clawed frog)	LOC100498108	XP_002937395.2
Bos taurus (cattle)	SLC12A9	NP_001193215.1
Drosophila melanogaster (fruit fly)	CG10413	NP_609887.1
Schizosaccharomyces pombe (fission yeast)	can1	NP_595740.1
Neurospora crassa	NCU03784	XP_961130.1
Pan troglodytes (chimpanzee)	SLC12A9	XP_003318707.1
Canis lupus familiaris (dog)	SLC12A9	XP_005621083.1
Rattus norvegicus (Norway rat)	Slc12a9	NP_599232.1
Magnaporthe oryzae (rice blast fungus)	MGG_16806	XP_003712279.1

Gene Ontology
Bibliography

Related articles in PubMed

Expression and regulation of drug transporters in vertebrate neutrophils.
Foulkes MJ, Henry KM, Rougeot J, Hooper-Greenhill E, Loynes CA, Jeffrey P, Fleming A, Savage CO, Meijer AH, Jones S, Renshaw SA
Scientific reports7(1)4967(2017 Jul)

Evolution of complexity in the zebrafish synapse proteome.
Bay?s ?, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 Mar)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following SLC12A9 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SLC12A9 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa03788	NM_001128548.1 Latest version!	Danio rerio solute carrier family 12 member 9 (slc12a9), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
ODa03788	XM_021476318.1 Latest version!	Danio rerio solute carrier family 12, member 9 (slc12a9), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
ODa03788	XM_005165365.4 Latest version!	Danio rerio solute carrier family 12, member 9 (slc12a9), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
ODa03788	XM_017356172.1 Latest version!	Danio rerio solute carrier family 12, member 9 (slc12a9), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
View Old Accession Versions >>
ODa03788	XM_005165365.3	Danio rerio solute carrier family 12, member 9 (slc12a9), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SLC12A9 ( NM_001128548.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> NP_001122020.1 Danio rerio solute carrier family 12 member 9 (slc12a9), mRNA.

Price & Availability↑

CloneID	ODa03788
Clone ID Related Accession (Same CDS sequence)	XM_005165365.4 , NM_001128548.1 , XM_017356172.1 , XM_021476318.1 , XM_005165365.3
Accession Version	NM_001128548.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2700bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-07
Organism	Danio rerio(zebrafish)
Product	solute carrier family 12 member 9
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CT025690.7 and BX294181.5. On Jul 31, 2008 this sequence version replaced XM_001333041.2. ##Evidence-Data-START## CDS exon combination :: GDQH01009196.1, GFIL01038234.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGGCAAATG AGCACTCGCC TCTTCTGGTT CATGGGGTTT ACAGCATGAT GGGAAATGCT 
GAAGACTCCA GGGGTGGATC AGCAGGAACA GGAGAGGCGT CAAACCCCAA AACAGACCCC 
CGAAAACTCA ACACCTTCTT TGGCGTCATG GTGCCCACCA TTCTCTCCAT GTTCAGCATT 
GTGCTCTTTC TTCGTACAGG TTTTGTTGTG GGACATGCTG GTCTTCTGCA TGGTCTCCTC 
ATGCTGTTTG TGGCTTACTT CATCATCTCA TTGACAATCC TGTCTATATG TGCCATTTCA 
ACTAATGGGG CTGTGGAAGG AGGTGGAGCT TATTTCATGA TCAGTCGTTC TCTGGGTCCA 
GAGTTTGGCG GGAGCATCGG CCTGATGTTT TACCTTGCGA AGGTGTGTGC GTGTGGTGTG 
TATGTTCTCG GGCTGGTGGA GGCCATAATG GACGTCTTCG GTCAGGATCC AGGCTCTTCT 
GTGGCACAGG GGCTGCGTGT GTTGCCGCAG GGCTACTGGT ACACCGTGTT GTACTCCTCT 
GTAGTGTTGT TGCTGTGTAT GCTGGTGTGT CTGGTCGGTG CTCACATCTA CGCCAAAGCC 
TCTTTTCTCA TTCTGCTGGT GGTGACGGTG TCTCTGATCT CCATTATCAT CAGCCCACTG 
ATAGTCAGTC CACAGGGCTT TAACATCACG CACACGTACG GCAACAACCA CAGTGTTACG 
GTCAGTCCAA GCTACACGGG CTTTAACAGC ACCACTCTGA AGAACAACCT TGGACCTCGC 
TACTCTTTGG ACTACAGTAC CAACACAATG ATGTCTTTCG CCACTGTGTT TGCTGTGATG 
TTCACCAGCT GCACAGGCAT CATGGCAGGA GCCAACATGT CGGGTGAGCT CAAGAATCCC 
AGTGAATCAA TACCTAAAGG GACCATTATG GCGGTGGCGT ACACCTTCAC TGTCTATGTG 
CTTCTCTATC TCCTGCTGAG CTCTACCTGT GACAGGTCGT TGTTACTCAA TGATTACGCA 
GTGTTTCAGA GAGTGAACGT GTGGCCTCCA TTCGTGACCA TCGGCGTTTA CTGCGCTTCT 
CTCTCAGCTG CCATGTGCTC TATGATAGGA GCCTCTCGAA TCCTCCATGC TCTTGCCCTG 
GACCAACTCT TTGGTCTTCC TTTGGCTCCA GCTGCTGTCA CCTCCAGTTC GGGGAACCCA 
TGGGTCTCTG TGCTCTACAC CTGGGCTCTG GTGCAGTGTA CGCTGTTTGC AGGGCAGCTG 
AATGTGATTG CTGGTATTGT GACAGTGTTT TATCTGTTGG CGTATGCTGC GGTTGATTTG 
GCCTGTCTTG CTCTGGAATG GGCATCTGCT CCAAACTTCA GGCCGACGTT CCAGTTTTTC 
TCCTGGCACA CGTGTCTGTT GGGTATCATC AGCTGTGTGG TGATGATGTT CGTGATTAAC 
CCTGTGTATT CCTCAGCCAG TATTGTGCTG CTGCTGCTCC TGCTGCTCTT CCTGCATTAC 
CGATCTCCCA CCAGCAGCTG GGGCTACATC AGCCAAGCCC TCATCTTCCA TCAGGTGAGG 
AAGTACCTTC TGATGTTGGA CTCTCGGAAG GATCATGTGA AGTTCTGGCG GCCGCAGGTG 
TTACTGATGG TGTCCAACCC ACGCTCCTCC TGTCAGCTCA TCTGCTTTGT CAACCAGCTG 
AAGAAAGGGG GCCTGTTTGT CCTTGGACAC GTGCAGATTG GAGATCTGGA TGTGCTGCCA 
GCAGACCCGG TGCAGCCGCA GTATAATTTC TGGTTGAGTC TGGTGGATAA GCTGGGTGTG 
AAGGCCTTTG TGGATCTGAC TCTTTCTCCC TCGGTCAGGC AGGGCACTCA ACATCTGCTC 
CGCATCACTG GACTTGGAGG AATGAAGCCT AATACCCTGG TGTTGGGCTT TTATGACAAC 
TCCTATCCAG AGGACTACTT CCTCCAGGAC CCTGTCTTTT GCAAGGGCGA CAGATCTGAA 
GGGGATAATT TCGGTGTGGA CCTCCCATCC CTCCAGGCTC ATTTTCCACC GGTGCGCCAT 
GCCGAGAGCC CTCGCGCACT GCAGCCTCAG GAGTACGTCA GCATCATTCA GGATGCCATC 
AAAATGGGCA AGAACATTTG CTTGGCGCGC TACTTCTTCC AGCTGCCGCC CGAGAGCAAA 
GGTGCAACGT ACATGTGGGG AAAAGACAGC ATGGACACCA TCGACGTGTG GCCGACAAAC 
CTCTTGACTC CAGGTAGTGC CAGTTACGCT GACGTGGGCA GTCTGTTCCT GCTTCAGATG 
GCGTGTGTGT TGAACATGGC CAGCGGGTGG CGCCGTGCGA GATTGCGTAT ATTCGTGTGT 
GTGGAGTCTG AATCGGAAGA TCAGGGCTGG CTGGCCAAAG AGGAGCAGTT TAGAGAGTTG 
CTGGGAAAGC TGAGGATTCG TGCTGCTATT AAGATCGTGG CGTGGGATAA TGTCGCACGC 
ATGGTTAGAG GGCCAAACAC AGAGAGCCAA CCGGTGTCAG AAGACTTCCT CTGTGCTGTG 
AATGGCCTTC TGAAGGAGCA CAGCTCGACT GCCGCCGTGC GCTTTCTGTA TTTGCCCGAT 
CCCCCATCTA GTTGTGAGCT GTCCCAACAG TACCTCACCC AGCTAGACAC ACTGACCCGG 
GATCTGGGCC CGACGCTCTT GATTCATGGG GTTACCCCAG TCACGTGCAC TGAGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001122020.1
CDS	1..2700
Translation	MANEHSPLLVHGVYSMMGNAEDSRGGSAGTGEASNPKTDPRKLNTFFGVMVPTILSMFSIVLFLRTGFVVGHAGLLHGLLMLFVAYFIISLTILSICAISTNGAVEGGGAYFMISRSLGPEFGGSIGLMFYLAKVCACGVYVLGLVEAIMDVFGQDPGSSVAQGLRVLPQGYWYTVLYSSVVLLLCMLVCLVGAHIYAKASFLILLVVTVSLISIIISPLIVSPQGFNITHTYGNNHSVTVSPSYTGFNSTTLKNNLGPRYSLDYSTNTMMSFATVFAVMFTSCTGIMAGANMSGELKNPSESIPKGTIMAVAYTFTVYVLLYLLLSSTCDRSLLLNDYAVFQRVNVWPPFVTIGVYCASLSAAMCSMIGASRILHALALDQLFGLPLAPAAVTSSSGNPWVSVLYTWALVQCTLFAGQLNVIAGIVTVFYLLAYAAVDLACLALEWASAPNFRPTFQFFSWHTCLLGIISCVVMMFVINPVYSSASIVLLLLLLLFLHYRSPTSSWGYISQALIFHQVRKYLLMLDSRKDHVKFWRPQVLLMVSNPRSSCQLICFVNQLKKGGLFVLGHVQIGDLDVLPADPVQPQYNFWLSLVDKLGVKAFVDLTLSPSVRQGTQHLLRITGLGGMKPNTLVLGFYDNSYPEDYFLQDPVFCKGDRSEGDNFGVDLPSLQAHFPPVRHAESPRALQPQEYVSIIQDAIKMGKNICLARYFFQLPPESKGATYMWGKDSMDTIDVWPTNLLTPGSASYADVGSLFLLQMACVLNMASGWRRARLRIFVCVESESEDQGWLAKEEQFRELLGKLRIRAAIKIVAWDNVARMVRGPNTESQPVSEDFLCAVNGLLKEHSSTAAVRFLYLPDPPSSCELSQQYLTQLDTLTRDLGPTLLIHGVTPVTCTEL

Target ORF information:

RefSeq Version	NM_001128548.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio solute carrier family 12 member 9 (slc12a9), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001128548.1

ORF Insert Sequence:

ATGGCAAATG AGCACTCGCC TCTTCTGGTT CATGGGGTTT ACAGCATGAT GGGAAATGCT 
GAAGACTCCA GGGGTGGATC AGCAGGAACA GGAGAGGCGT CAAACCCCAA AACAGACCCC 
CGAAAACTCA ACACCTTCTT TGGCGTCATG GTGCCCACCA TTCTCTCCAT GTTCAGCATT 
GTGCTCTTTC TTCGTACAGG TTTTGTTGTG GGACATGCTG GTCTTCTGCA TGGTCTCCTC 
ATGCTGTTTG TGGCTTACTT CATCATCTCA TTGACAATCC TGTCTATATG TGCCATTTCA 
ACTAATGGGG CTGTGGAAGG AGGTGGAGCT TATTTCATGA TCAGTCGTTC TCTGGGTCCA 
GAGTTTGGCG GGAGCATCGG CCTGATGTTT TACCTTGCGA AGGTGTGTGC GTGTGGTGTG 
TATGTTCTCG GGCTGGTGGA GGCCATAATG GACGTCTTCG GTCAGGATCC AGGCTCTTCT 
GTGGCACAGG GGCTGCGTGT GTTGCCGCAG GGCTACTGGT ACACCGTGTT GTACTCCTCT 
GTAGTGTTGT TGCTGTGTAT GCTGGTGTGT CTGGTCGGTG CTCACATCTA CGCCAAAGCC 
TCTTTTCTCA TTCTGCTGGT GGTGACGGTG TCTCTGATCT CCATTATCAT CAGCCCACTG 
ATAGTCAGTC CACAGGGCTT TAACATCACG CACACGTACG GCAACAACCA CAGTGTTACG 
GTCAGTCCAA GCTACACGGG CTTTAACAGC ACCACTCTGA AGAACAACCT TGGACCTCGC 
TACTCTTTGG ACTACAGTAC CAACACAATG ATGTCTTTCG CCACTGTGTT TGCTGTGATG 
TTCACCAGCT GCACAGGCAT CATGGCAGGA GCCAACATGT CGGGTGAGCT CAAGAATCCC 
AGTGAATCAA TACCTAAAGG GACCATTATG GCGGTGGCGT ACACCTTCAC TGTCTATGTG 
CTTCTCTATC TCCTGCTGAG CTCTACCTGT GACAGGTCGT TGTTACTCAA TGATTACGCA 
GTGTTTCAGA GAGTGAACGT GTGGCCTCCA TTCGTGACCA TCGGCGTTTA CTGCGCTTCT 
CTCTCAGCTG CCATGTGCTC TATGATAGGA GCCTCTCGAA TCCTCCATGC TCTTGCCCTG 
GACCAACTCT TTGGTCTTCC TTTGGCTCCA GCTGCTGTCA CCTCCAGTTC GGGGAACCCA 
TGGGTCTCTG TGCTCTACAC CTGGGCTCTG GTGCAGTGTA CGCTGTTTGC AGGGCAGCTG 
AATGTGATTG CTGGTATTGT GACAGTGTTT TATCTGTTGG CGTATGCTGC GGTTGATTTG 
GCCTGTCTTG CTCTGGAATG GGCATCTGCT CCAAACTTCA GGCCGACGTT CCAGTTTTTC 
TCCTGGCACA CGTGTCTGTT GGGTATCATC AGCTGTGTGG TGATGATGTT CGTGATTAAC 
CCTGTGTATT CCTCAGCCAG TATTGTGCTG CTGCTGCTCC TGCTGCTCTT CCTGCATTAC 
CGATCTCCCA CCAGCAGCTG GGGCTACATC AGCCAAGCCC TCATCTTCCA TCAGGTGAGG 
AAGTACCTTC TGATGTTGGA CTCTCGGAAG GATCATGTGA AGTTCTGGCG GCCGCAGGTG 
TTACTGATGG TGTCCAACCC ACGCTCCTCC TGTCAGCTCA TCTGCTTTGT CAACCAGCTG 
AAGAAAGGGG GCCTGTTTGT CCTTGGACAC GTGCAGATTG GAGATCTGGA TGTGCTGCCA 
GCAGACCCGG TGCAGCCGCA GTATAATTTC TGGTTGAGTC TGGTGGATAA GCTGGGTGTG 
AAGGCCTTTG TGGATCTGAC TCTTTCTCCC TCGGTCAGGC AGGGCACTCA ACATCTGCTC 
CGCATCACTG GACTTGGAGG AATGAAGCCT AATACCCTGG TGTTGGGCTT TTATGACAAC 
TCCTATCCAG AGGACTACTT CCTCCAGGAC CCTGTCTTTT GCAAGGGCGA CAGATCTGAA 
GGGGATAATT TCGGTGTGGA CCTCCCATCC CTCCAGGCTC ATTTTCCACC GGTGCGCCAT 
GCCGAGAGCC CTCGCGCACT GCAGCCTCAG GAGTACGTCA GCATCATTCA GGATGCCATC 
AAAATGGGCA AGAACATTTG CTTGGCGCGC TACTTCTTCC AGCTGCCGCC CGAGAGCAAA 
GGTGCAACGT ACATGTGGGG AAAAGACAGC ATGGACACCA TCGACGTGTG GCCGACAAAC 
CTCTTGACTC CAGGTAGTGC CAGTTACGCT GACGTGGGCA GTCTGTTCCT GCTTCAGATG 
GCGTGTGTGT TGAACATGGC CAGCGGGTGG CGCCGTGCGA GATTGCGTAT ATTCGTGTGT 
GTGGAGTCTG AATCGGAAGA TCAGGGCTGG CTGGCCAAAG AGGAGCAGTT TAGAGAGTTG 
CTGGGAAAGC TGAGGATTCG TGCTGCTATT AAGATCGTGG CGTGGGATAA TGTCGCACGC 
ATGGTTAGAG GGCCAAACAC AGAGAGCCAA CCGGTGTCAG AAGACTTCCT CTGTGCTGTG 
AATGGCCTTC TGAAGGAGCA CAGCTCGACT GCCGCCGTGC GCTTTCTGTA TTTGCCCGAT 
CCCCCATCTA GTTGTGAGCT GTCCCAACAG TACCTCACCC AGCTAGACAC ACTGACCCGG 
GATCTGGGCC CGACGCTCTT GATTCATGGG GTTACCCCAG TCACGTGCAC TGAGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SLC12A9 ( XM_005165365.4 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005165422.1 Danio rerio solute carrier family 12, member 9 (slc12a9), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa03788
Clone ID Related Accession (Same CDS sequence)	XM_005165365.4 , NM_001128548.1 , XM_017356172.1 , XM_021476318.1 , XM_005165365.3
Accession Version	XM_005165365.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2700bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	solute carrier family 12 member 9 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005165365.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAATG AGCACTCGCC TCTTCTGGTT CATGGGGTTT ACAGCATGAT GGGAAATGCT 
GAAGACTCCA GGGGTGGATC AGCAGGAACA GGAGAGGCGT CAAACCCCAA AACAGACCCC 
CGAAAACTCA ACACCTTCTT TGGCGTCATG GTGCCCACCA TTCTCTCCAT GTTCAGCATT 
GTGCTCTTTC TTCGTACAGG TTTTGTTGTG GGACATGCTG GTCTTCTGCA TGGTCTCCTC 
ATGCTGTTTG TGGCTTACTT CATCATCTCA TTGACAATCC TGTCTATATG TGCCATTTCA 
ACTAATGGGG CTGTGGAAGG AGGTGGAGCT TATTTCATGA TCAGTCGTTC TCTGGGTCCA 
GAGTTTGGCG GGAGCATCGG CCTGATGTTT TACCTTGCGA AGGTGTGTGC GTGTGGTGTG 
TATGTTCTCG GGCTGGTGGA GGCCATAATG GACGTCTTCG GTCAGGATCC AGGCTCTTCT 
GTGGCACAGG GGCTGCGTGT GTTGCCGCAG GGCTACTGGT ACACCGTGTT GTACTCCTCT 
GTAGTGTTGT TGCTGTGTAT GCTGGTGTGT CTGGTCGGTG CTCACATCTA CGCCAAAGCC 
TCTTTTCTCA TTCTGCTGGT GGTGACGGTG TCTCTGATCT CCATTATCAT CAGCCCACTG 
ATAGTCAGTC CACAGGGCTT TAACATCACG CACACGTACG GCAACAACCA CAGTGTTACG 
GTCAGTCCAA GCTACACGGG CTTTAACAGC ACCACTCTGA AGAACAACCT TGGACCTCGC 
TACTCTTTGG ACTACAGTAC CAACACAATG ATGTCTTTCG CCACTGTGTT TGCTGTGATG 
TTCACCAGCT GCACAGGCAT CATGGCAGGA GCCAACATGT CGGGTGAGCT CAAGAATCCC 
AGTGAATCAA TACCTAAAGG GACCATTATG GCGGTGGCGT ACACCTTCAC TGTCTATGTG 
CTTCTCTATC TCCTGCTGAG CTCTACCTGT GACAGGTCGT TGTTACTCAA TGATTACGCA 
GTGTTTCAGA GAGTGAACGT GTGGCCTCCA TTCGTGACCA TCGGCGTTTA CTGCGCTTCT 
CTCTCAGCTG CCATGTGCTC TATGATAGGA GCCTCTCGAA TCCTCCATGC TCTTGCCCTG 
GACCAACTCT TTGGTCTTCC TTTGGCTCCA GCTGCTGTCA CCTCCAGTTC GGGGAACCCA 
TGGGTCTCTG TGCTCTACAC CTGGGCTCTG GTGCAGTGTA CGCTGTTTGC AGGGCAGCTG 
AATGTGATTG CTGGTATTGT GACAGTGTTT TATCTGTTGG CGTATGCTGC GGTTGATTTG 
GCCTGTCTTG CTCTGGAATG GGCATCTGCT CCAAACTTCA GGCCGACGTT CCAGTTTTTC 
TCCTGGCACA CGTGTCTGTT GGGTATCATC AGCTGTGTGG TGATGATGTT CGTGATTAAC 
CCTGTGTATT CCTCAGCCAG TATTGTGCTG CTGCTGCTCC TGCTGCTCTT CCTGCATTAC 
CGATCTCCCA CCAGCAGCTG GGGCTACATC AGCCAAGCCC TCATCTTCCA TCAGGTGAGG 
AAGTACCTTC TGATGTTGGA CTCTCGGAAG GATCATGTGA AGTTCTGGCG GCCGCAGGTG 
TTACTGATGG TGTCCAACCC ACGCTCCTCC TGTCAGCTCA TCTGCTTTGT CAACCAGCTG 
AAGAAAGGGG GCCTGTTTGT CCTTGGACAC GTGCAGATTG GAGATCTGGA TGTGCTGCCA 
GCAGACCCGG TGCAGCCGCA GTATAATTTC TGGTTGAGTC TGGTGGATAA GCTGGGTGTG 
AAGGCCTTTG TGGATCTGAC TCTTTCTCCC TCGGTCAGGC AGGGCACTCA ACATCTGCTC 
CGCATCACTG GACTTGGAGG AATGAAGCCT AATACCCTGG TGTTGGGCTT TTATGACAAC 
TCCTATCCAG AGGACTACTT CCTCCAGGAC CCTGTCTTTT GCAAGGGCGA CAGATCTGAA 
GGGGATAATT TCGGTGTGGA CCTCCCATCC CTCCAGGCTC ATTTTCCACC GGTGCGCCAT 
GCCGAGAGCC CTCGCGCACT GCAGCCTCAG GAGTACGTCA GCATCATTCA GGATGCCATC 
AAAATGGGCA AGAACATTTG CTTGGCGCGC TACTTCTTCC AGCTGCCGCC CGAGAGCAAA 
GGTGCAACGT ACATGTGGGG AAAAGACAGC ATGGACACCA TCGACGTGTG GCCGACAAAC 
CTCTTGACTC CAGGTAGTGC CAGTTACGCT GACGTGGGCA GTCTGTTCCT GCTTCAGATG 
GCGTGTGTGT TGAACATGGC CAGCGGGTGG CGCCGTGCGA GATTGCGTAT ATTCGTGTGT 
GTGGAGTCTG AATCGGAAGA TCAGGGCTGG CTGGCCAAAG AGGAGCAGTT TAGAGAGTTG 
CTGGGAAAGC TGAGGATTCG TGCTGCTATT AAGATCGTGG CGTGGGATAA TGTCGCACGC 
ATGGTTAGAG GGCCAAACAC AGAGAGCCAA CCGGTGTCAG AAGACTTCCT CTGTGCTGTG 
AATGGCCTTC TGAAGGAGCA CAGCTCGACT GCCGCCGTGC GCTTTCTGTA TTTGCCCGAT 
CCCCCATCTA GTTGTGAGCT GTCCCAACAG TACCTCACCC AGCTAGACAC ACTGACCCGG 
GATCTGGGCC CGACGCTCTT GATTCATGGG GTTACCCCAG TCACGTGCAC TGAGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005165422.1
CDS	368..3067
Translation	MANEHSPLLVHGVYSMMGNAEDSRGGSAGTGEASNPKTDPRKLNTFFGVMVPTILSMFSIVLFLRTGFVVGHAGLLHGLLMLFVAYFIISLTILSICAISTNGAVEGGGAYFMISRSLGPEFGGSIGLMFYLAKVCACGVYVLGLVEAIMDVFGQDPGSSVAQGLRVLPQGYWYTVLYSSVVLLLCMLVCLVGAHIYAKASFLILLVVTVSLISIIISPLIVSPQGFNITHTYGNNHSVTVSPSYTGFNSTTLKNNLGPRYSLDYSTNTMMSFATVFAVMFTSCTGIMAGANMSGELKNPSESIPKGTIMAVAYTFTVYVLLYLLLSSTCDRSLLLNDYAVFQRVNVWPPFVTIGVYCASLSAAMCSMIGASRILHALALDQLFGLPLAPAAVTSSSGNPWVSVLYTWALVQCTLFAGQLNVIAGIVTVFYLLAYAAVDLACLALEWASAPNFRPTFQFFSWHTCLLGIISCVVMMFVINPVYSSASIVLLLLLLLFLHYRSPTSSWGYISQALIFHQVRKYLLMLDSRKDHVKFWRPQVLLMVSNPRSSCQLICFVNQLKKGGLFVLGHVQIGDLDVLPADPVQPQYNFWLSLVDKLGVKAFVDLTLSPSVRQGTQHLLRITGLGGMKPNTLVLGFYDNSYPEDYFLQDPVFCKGDRSEGDNFGVDLPSLQAHFPPVRHAESPRALQPQEYVSIIQDAIKMGKNICLARYFFQLPPESKGATYMWGKDSMDTIDVWPTNLLTPGSASYADVGSLFLLQMACVLNMASGWRRARLRIFVCVESESEDQGWLAKEEQFRELLGKLRIRAAIKIVAWDNVARMVRGPNTESQPVSEDFLCAVNGLLKEHSSTAAVRFLYLPDPPSSCELSQQYLTQLDTLTRDLGPTLLIHGVTPVTCTEL

Target ORF information:

RefSeq Version	XM_005165365.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio solute carrier family 12, member 9 (slc12a9), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005165365.4

ORF Insert Sequence:

ATGGCAAATG AGCACTCGCC TCTTCTGGTT CATGGGGTTT ACAGCATGAT GGGAAATGCT 
GAAGACTCCA GGGGTGGATC AGCAGGAACA GGAGAGGCGT CAAACCCCAA AACAGACCCC 
CGAAAACTCA ACACCTTCTT TGGCGTCATG GTGCCCACCA TTCTCTCCAT GTTCAGCATT 
GTGCTCTTTC TTCGTACAGG TTTTGTTGTG GGACATGCTG GTCTTCTGCA TGGTCTCCTC 
ATGCTGTTTG TGGCTTACTT CATCATCTCA TTGACAATCC TGTCTATATG TGCCATTTCA 
ACTAATGGGG CTGTGGAAGG AGGTGGAGCT TATTTCATGA TCAGTCGTTC TCTGGGTCCA 
GAGTTTGGCG GGAGCATCGG CCTGATGTTT TACCTTGCGA AGGTGTGTGC GTGTGGTGTG 
TATGTTCTCG GGCTGGTGGA GGCCATAATG GACGTCTTCG GTCAGGATCC AGGCTCTTCT 
GTGGCACAGG GGCTGCGTGT GTTGCCGCAG GGCTACTGGT ACACCGTGTT GTACTCCTCT 
GTAGTGTTGT TGCTGTGTAT GCTGGTGTGT CTGGTCGGTG CTCACATCTA CGCCAAAGCC 
TCTTTTCTCA TTCTGCTGGT GGTGACGGTG TCTCTGATCT CCATTATCAT CAGCCCACTG 
ATAGTCAGTC CACAGGGCTT TAACATCACG CACACGTACG GCAACAACCA CAGTGTTACG 
GTCAGTCCAA GCTACACGGG CTTTAACAGC ACCACTCTGA AGAACAACCT TGGACCTCGC 
TACTCTTTGG ACTACAGTAC CAACACAATG ATGTCTTTCG CCACTGTGTT TGCTGTGATG 
TTCACCAGCT GCACAGGCAT CATGGCAGGA GCCAACATGT CGGGTGAGCT CAAGAATCCC 
AGTGAATCAA TACCTAAAGG GACCATTATG GCGGTGGCGT ACACCTTCAC TGTCTATGTG 
CTTCTCTATC TCCTGCTGAG CTCTACCTGT GACAGGTCGT TGTTACTCAA TGATTACGCA 
GTGTTTCAGA GAGTGAACGT GTGGCCTCCA TTCGTGACCA TCGGCGTTTA CTGCGCTTCT 
CTCTCAGCTG CCATGTGCTC TATGATAGGA GCCTCTCGAA TCCTCCATGC TCTTGCCCTG 
GACCAACTCT TTGGTCTTCC TTTGGCTCCA GCTGCTGTCA CCTCCAGTTC GGGGAACCCA 
TGGGTCTCTG TGCTCTACAC CTGGGCTCTG GTGCAGTGTA CGCTGTTTGC AGGGCAGCTG 
AATGTGATTG CTGGTATTGT GACAGTGTTT TATCTGTTGG CGTATGCTGC GGTTGATTTG 
GCCTGTCTTG CTCTGGAATG GGCATCTGCT CCAAACTTCA GGCCGACGTT CCAGTTTTTC 
TCCTGGCACA CGTGTCTGTT GGGTATCATC AGCTGTGTGG TGATGATGTT CGTGATTAAC 
CCTGTGTATT CCTCAGCCAG TATTGTGCTG CTGCTGCTCC TGCTGCTCTT CCTGCATTAC 
CGATCTCCCA CCAGCAGCTG GGGCTACATC AGCCAAGCCC TCATCTTCCA TCAGGTGAGG 
AAGTACCTTC TGATGTTGGA CTCTCGGAAG GATCATGTGA AGTTCTGGCG GCCGCAGGTG 
TTACTGATGG TGTCCAACCC ACGCTCCTCC TGTCAGCTCA TCTGCTTTGT CAACCAGCTG 
AAGAAAGGGG GCCTGTTTGT CCTTGGACAC GTGCAGATTG GAGATCTGGA TGTGCTGCCA 
GCAGACCCGG TGCAGCCGCA GTATAATTTC TGGTTGAGTC TGGTGGATAA GCTGGGTGTG 
AAGGCCTTTG TGGATCTGAC TCTTTCTCCC TCGGTCAGGC AGGGCACTCA ACATCTGCTC 
CGCATCACTG GACTTGGAGG AATGAAGCCT AATACCCTGG TGTTGGGCTT TTATGACAAC 
TCCTATCCAG AGGACTACTT CCTCCAGGAC CCTGTCTTTT GCAAGGGCGA CAGATCTGAA 
GGGGATAATT TCGGTGTGGA CCTCCCATCC CTCCAGGCTC ATTTTCCACC GGTGCGCCAT 
GCCGAGAGCC CTCGCGCACT GCAGCCTCAG GAGTACGTCA GCATCATTCA GGATGCCATC 
AAAATGGGCA AGAACATTTG CTTGGCGCGC TACTTCTTCC AGCTGCCGCC CGAGAGCAAA 
GGTGCAACGT ACATGTGGGG AAAAGACAGC ATGGACACCA TCGACGTGTG GCCGACAAAC 
CTCTTGACTC CAGGTAGTGC CAGTTACGCT GACGTGGGCA GTCTGTTCCT GCTTCAGATG 
GCGTGTGTGT TGAACATGGC CAGCGGGTGG CGCCGTGCGA GATTGCGTAT ATTCGTGTGT 
GTGGAGTCTG AATCGGAAGA TCAGGGCTGG CTGGCCAAAG AGGAGCAGTT TAGAGAGTTG 
CTGGGAAAGC TGAGGATTCG TGCTGCTATT AAGATCGTGG CGTGGGATAA TGTCGCACGC 
ATGGTTAGAG GGCCAAACAC AGAGAGCCAA CCGGTGTCAG AAGACTTCCT CTGTGCTGTG 
AATGGCCTTC TGAAGGAGCA CAGCTCGACT GCCGCCGTGC GCTTTCTGTA TTTGCCCGAT 
CCCCCATCTA GTTGTGAGCT GTCCCAACAG TACCTCACCC AGCTAGACAC ACTGACCCGG 
GATCTGGGCC CGACGCTCTT GATTCATGGG GTTACCCCAG TCACGTGCAC TGAGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SLC12A9 ( XM_017356172.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_017211661.1 Danio rerio solute carrier family 12, member 9 (slc12a9), transcript variant X2, mRNA.

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CloneID	ODa03788
Clone ID Related Accession (Same CDS sequence)	XM_005165365.4 , NM_001128548.1 , XM_017356172.1 , XM_021476318.1 , XM_005165365.3
Accession Version	XM_017356172.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2700bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	solute carrier family 12 member 9 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAATG AGCACTCGCC TCTTCTGGTT CATGGGGTTT ACAGCATGAT GGGAAATGCT 
GAAGACTCCA GGGGTGGATC AGCAGGAACA GGAGAGGCGT CAAACCCCAA AACAGACCCC 
CGAAAACTCA ACACCTTCTT TGGCGTCATG GTGCCCACCA TTCTCTCCAT GTTCAGCATT 
GTGCTCTTTC TTCGTACAGG TTTTGTTGTG GGACATGCTG GTCTTCTGCA TGGTCTCCTC 
ATGCTGTTTG TGGCTTACTT CATCATCTCA TTGACAATCC TGTCTATATG TGCCATTTCA 
ACTAATGGGG CTGTGGAAGG AGGTGGAGCT TATTTCATGA TCAGTCGTTC TCTGGGTCCA 
GAGTTTGGCG GGAGCATCGG CCTGATGTTT TACCTTGCGA AGGTGTGTGC GTGTGGTGTG 
TATGTTCTCG GGCTGGTGGA GGCCATAATG GACGTCTTCG GTCAGGATCC AGGCTCTTCT 
GTGGCACAGG GGCTGCGTGT GTTGCCGCAG GGCTACTGGT ACACCGTGTT GTACTCCTCT 
GTAGTGTTGT TGCTGTGTAT GCTGGTGTGT CTGGTCGGTG CTCACATCTA CGCCAAAGCC 
TCTTTTCTCA TTCTGCTGGT GGTGACGGTG TCTCTGATCT CCATTATCAT CAGCCCACTG 
ATAGTCAGTC CACAGGGCTT TAACATCACG CACACGTACG GCAACAACCA CAGTGTTACG 
GTCAGTCCAA GCTACACGGG CTTTAACAGC ACCACTCTGA AGAACAACCT TGGACCTCGC 
TACTCTTTGG ACTACAGTAC CAACACAATG ATGTCTTTCG CCACTGTGTT TGCTGTGATG 
TTCACCAGCT GCACAGGCAT CATGGCAGGA GCCAACATGT CGGGTGAGCT CAAGAATCCC 
AGTGAATCAA TACCTAAAGG GACCATTATG GCGGTGGCGT ACACCTTCAC TGTCTATGTG 
CTTCTCTATC TCCTGCTGAG CTCTACCTGT GACAGGTCGT TGTTACTCAA TGATTACGCA 
GTGTTTCAGA GAGTGAACGT GTGGCCTCCA TTCGTGACCA TCGGCGTTTA CTGCGCTTCT 
CTCTCAGCTG CCATGTGCTC TATGATAGGA GCCTCTCGAA TCCTCCATGC TCTTGCCCTG 
GACCAACTCT TTGGTCTTCC TTTGGCTCCA GCTGCTGTCA CCTCCAGTTC GGGGAACCCA 
TGGGTCTCTG TGCTCTACAC CTGGGCTCTG GTGCAGTGTA CGCTGTTTGC AGGGCAGCTG 
AATGTGATTG CTGGTATTGT GACAGTGTTT TATCTGTTGG CGTATGCTGC GGTTGATTTG 
GCCTGTCTTG CTCTGGAATG GGCATCTGCT CCAAACTTCA GGCCGACGTT CCAGTTTTTC 
TCCTGGCACA CGTGTCTGTT GGGTATCATC AGCTGTGTGG TGATGATGTT CGTGATTAAC 
CCTGTGTATT CCTCAGCCAG TATTGTGCTG CTGCTGCTCC TGCTGCTCTT CCTGCATTAC 
CGATCTCCCA CCAGCAGCTG GGGCTACATC AGCCAAGCCC TCATCTTCCA TCAGGTGAGG 
AAGTACCTTC TGATGTTGGA CTCTCGGAAG GATCATGTGA AGTTCTGGCG GCCGCAGGTG 
TTACTGATGG TGTCCAACCC ACGCTCCTCC TGTCAGCTCA TCTGCTTTGT CAACCAGCTG 
AAGAAAGGGG GCCTGTTTGT CCTTGGACAC GTGCAGATTG GAGATCTGGA TGTGCTGCCA 
GCAGACCCGG TGCAGCCGCA GTATAATTTC TGGTTGAGTC TGGTGGATAA GCTGGGTGTG 
AAGGCCTTTG TGGATCTGAC TCTTTCTCCC TCGGTCAGGC AGGGCACTCA ACATCTGCTC 
CGCATCACTG GACTTGGAGG AATGAAGCCT AATACCCTGG TGTTGGGCTT TTATGACAAC 
TCCTATCCAG AGGACTACTT CCTCCAGGAC CCTGTCTTTT GCAAGGGCGA CAGATCTGAA 
GGGGATAATT TCGGTGTGGA CCTCCCATCC CTCCAGGCTC ATTTTCCACC GGTGCGCCAT 
GCCGAGAGCC CTCGCGCACT GCAGCCTCAG GAGTACGTCA GCATCATTCA GGATGCCATC 
AAAATGGGCA AGAACATTTG CTTGGCGCGC TACTTCTTCC AGCTGCCGCC CGAGAGCAAA 
GGTGCAACGT ACATGTGGGG AAAAGACAGC ATGGACACCA TCGACGTGTG GCCGACAAAC 
CTCTTGACTC CAGGTAGTGC CAGTTACGCT GACGTGGGCA GTCTGTTCCT GCTTCAGATG 
GCGTGTGTGT TGAACATGGC CAGCGGGTGG CGCCGTGCGA GATTGCGTAT ATTCGTGTGT 
GTGGAGTCTG AATCGGAAGA TCAGGGCTGG CTGGCCAAAG AGGAGCAGTT TAGAGAGTTG 
CTGGGAAAGC TGAGGATTCG TGCTGCTATT AAGATCGTGG CGTGGGATAA TGTCGCACGC 
ATGGTTAGAG GGCCAAACAC AGAGAGCCAA CCGGTGTCAG AAGACTTCCT CTGTGCTGTG 
AATGGCCTTC TGAAGGAGCA CAGCTCGACT GCCGCCGTGC GCTTTCTGTA TTTGCCCGAT 
CCCCCATCTA GTTGTGAGCT GTCCCAACAG TACCTCACCC AGCTAGACAC ACTGACCCGG 
GATCTGGGCC CGACGCTCTT GATTCATGGG GTTACCCCAG TCACGTGCAC TGAGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017211661.1
CDS	515..3214
Translation	MANEHSPLLVHGVYSMMGNAEDSRGGSAGTGEASNPKTDPRKLNTFFGVMVPTILSMFSIVLFLRTGFVVGHAGLLHGLLMLFVAYFIISLTILSICAISTNGAVEGGGAYFMISRSLGPEFGGSIGLMFYLAKVCACGVYVLGLVEAIMDVFGQDPGSSVAQGLRVLPQGYWYTVLYSSVVLLLCMLVCLVGAHIYAKASFLILLVVTVSLISIIISPLIVSPQGFNITHTYGNNHSVTVSPSYTGFNSTTLKNNLGPRYSLDYSTNTMMSFATVFAVMFTSCTGIMAGANMSGELKNPSESIPKGTIMAVAYTFTVYVLLYLLLSSTCDRSLLLNDYAVFQRVNVWPPFVTIGVYCASLSAAMCSMIGASRILHALALDQLFGLPLAPAAVTSSSGNPWVSVLYTWALVQCTLFAGQLNVIAGIVTVFYLLAYAAVDLACLALEWASAPNFRPTFQFFSWHTCLLGIISCVVMMFVINPVYSSASIVLLLLLLLFLHYRSPTSSWGYISQALIFHQVRKYLLMLDSRKDHVKFWRPQVLLMVSNPRSSCQLICFVNQLKKGGLFVLGHVQIGDLDVLPADPVQPQYNFWLSLVDKLGVKAFVDLTLSPSVRQGTQHLLRITGLGGMKPNTLVLGFYDNSYPEDYFLQDPVFCKGDRSEGDNFGVDLPSLQAHFPPVRHAESPRALQPQEYVSIIQDAIKMGKNICLARYFFQLPPESKGATYMWGKDSMDTIDVWPTNLLTPGSASYADVGSLFLLQMACVLNMASGWRRARLRIFVCVESESEDQGWLAKEEQFRELLGKLRIRAAIKIVAWDNVARMVRGPNTESQPVSEDFLCAVNGLLKEHSSTAAVRFLYLPDPPSSCELSQQYLTQLDTLTRDLGPTLLIHGVTPVTCTEL

Target ORF information:

RefSeq Version	XM_017356172.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio solute carrier family 12, member 9 (slc12a9), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017356172.1

ORF Insert Sequence:

ATGGCAAATG AGCACTCGCC TCTTCTGGTT CATGGGGTTT ACAGCATGAT GGGAAATGCT 
GAAGACTCCA GGGGTGGATC AGCAGGAACA GGAGAGGCGT CAAACCCCAA AACAGACCCC 
CGAAAACTCA ACACCTTCTT TGGCGTCATG GTGCCCACCA TTCTCTCCAT GTTCAGCATT 
GTGCTCTTTC TTCGTACAGG TTTTGTTGTG GGACATGCTG GTCTTCTGCA TGGTCTCCTC 
ATGCTGTTTG TGGCTTACTT CATCATCTCA TTGACAATCC TGTCTATATG TGCCATTTCA 
ACTAATGGGG CTGTGGAAGG AGGTGGAGCT TATTTCATGA TCAGTCGTTC TCTGGGTCCA 
GAGTTTGGCG GGAGCATCGG CCTGATGTTT TACCTTGCGA AGGTGTGTGC GTGTGGTGTG 
TATGTTCTCG GGCTGGTGGA GGCCATAATG GACGTCTTCG GTCAGGATCC AGGCTCTTCT 
GTGGCACAGG GGCTGCGTGT GTTGCCGCAG GGCTACTGGT ACACCGTGTT GTACTCCTCT 
GTAGTGTTGT TGCTGTGTAT GCTGGTGTGT CTGGTCGGTG CTCACATCTA CGCCAAAGCC 
TCTTTTCTCA TTCTGCTGGT GGTGACGGTG TCTCTGATCT CCATTATCAT CAGCCCACTG 
ATAGTCAGTC CACAGGGCTT TAACATCACG CACACGTACG GCAACAACCA CAGTGTTACG 
GTCAGTCCAA GCTACACGGG CTTTAACAGC ACCACTCTGA AGAACAACCT TGGACCTCGC 
TACTCTTTGG ACTACAGTAC CAACACAATG ATGTCTTTCG CCACTGTGTT TGCTGTGATG 
TTCACCAGCT GCACAGGCAT CATGGCAGGA GCCAACATGT CGGGTGAGCT CAAGAATCCC 
AGTGAATCAA TACCTAAAGG GACCATTATG GCGGTGGCGT ACACCTTCAC TGTCTATGTG 
CTTCTCTATC TCCTGCTGAG CTCTACCTGT GACAGGTCGT TGTTACTCAA TGATTACGCA 
GTGTTTCAGA GAGTGAACGT GTGGCCTCCA TTCGTGACCA TCGGCGTTTA CTGCGCTTCT 
CTCTCAGCTG CCATGTGCTC TATGATAGGA GCCTCTCGAA TCCTCCATGC TCTTGCCCTG 
GACCAACTCT TTGGTCTTCC TTTGGCTCCA GCTGCTGTCA CCTCCAGTTC GGGGAACCCA 
TGGGTCTCTG TGCTCTACAC CTGGGCTCTG GTGCAGTGTA CGCTGTTTGC AGGGCAGCTG 
AATGTGATTG CTGGTATTGT GACAGTGTTT TATCTGTTGG CGTATGCTGC GGTTGATTTG 
GCCTGTCTTG CTCTGGAATG GGCATCTGCT CCAAACTTCA GGCCGACGTT CCAGTTTTTC 
TCCTGGCACA CGTGTCTGTT GGGTATCATC AGCTGTGTGG TGATGATGTT CGTGATTAAC 
CCTGTGTATT CCTCAGCCAG TATTGTGCTG CTGCTGCTCC TGCTGCTCTT CCTGCATTAC 
CGATCTCCCA CCAGCAGCTG GGGCTACATC AGCCAAGCCC TCATCTTCCA TCAGGTGAGG 
AAGTACCTTC TGATGTTGGA CTCTCGGAAG GATCATGTGA AGTTCTGGCG GCCGCAGGTG 
TTACTGATGG TGTCCAACCC ACGCTCCTCC TGTCAGCTCA TCTGCTTTGT CAACCAGCTG 
AAGAAAGGGG GCCTGTTTGT CCTTGGACAC GTGCAGATTG GAGATCTGGA TGTGCTGCCA 
GCAGACCCGG TGCAGCCGCA GTATAATTTC TGGTTGAGTC TGGTGGATAA GCTGGGTGTG 
AAGGCCTTTG TGGATCTGAC TCTTTCTCCC TCGGTCAGGC AGGGCACTCA ACATCTGCTC 
CGCATCACTG GACTTGGAGG AATGAAGCCT AATACCCTGG TGTTGGGCTT TTATGACAAC 
TCCTATCCAG AGGACTACTT CCTCCAGGAC CCTGTCTTTT GCAAGGGCGA CAGATCTGAA 
GGGGATAATT TCGGTGTGGA CCTCCCATCC CTCCAGGCTC ATTTTCCACC GGTGCGCCAT 
GCCGAGAGCC CTCGCGCACT GCAGCCTCAG GAGTACGTCA GCATCATTCA GGATGCCATC 
AAAATGGGCA AGAACATTTG CTTGGCGCGC TACTTCTTCC AGCTGCCGCC CGAGAGCAAA 
GGTGCAACGT ACATGTGGGG AAAAGACAGC ATGGACACCA TCGACGTGTG GCCGACAAAC 
CTCTTGACTC CAGGTAGTGC CAGTTACGCT GACGTGGGCA GTCTGTTCCT GCTTCAGATG 
GCGTGTGTGT TGAACATGGC CAGCGGGTGG CGCCGTGCGA GATTGCGTAT ATTCGTGTGT 
GTGGAGTCTG AATCGGAAGA TCAGGGCTGG CTGGCCAAAG AGGAGCAGTT TAGAGAGTTG 
CTGGGAAAGC TGAGGATTCG TGCTGCTATT AAGATCGTGG CGTGGGATAA TGTCGCACGC 
ATGGTTAGAG GGCCAAACAC AGAGAGCCAA CCGGTGTCAG AAGACTTCCT CTGTGCTGTG 
AATGGCCTTC TGAAGGAGCA CAGCTCGACT GCCGCCGTGC GCTTTCTGTA TTTGCCCGAT 
CCCCCATCTA GTTGTGAGCT GTCCCAACAG TACCTCACCC AGCTAGACAC ACTGACCCGG 
GATCTGGGCC CGACGCTCTT GATTCATGGG GTTACCCCAG TCACGTGCAC TGAGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SLC12A9 ( XM_021476318.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_021331993.1 Danio rerio solute carrier family 12, member 9 (slc12a9), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa03788
Clone ID Related Accession (Same CDS sequence)	XM_005165365.4 , NM_001128548.1 , XM_017356172.1 , XM_021476318.1 , XM_005165365.3
Accession Version	XM_021476318.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2700bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	solute carrier family 12 member 9 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAATG AGCACTCGCC TCTTCTGGTT CATGGGGTTT ACAGCATGAT GGGAAATGCT 
GAAGACTCCA GGGGTGGATC AGCAGGAACA GGAGAGGCGT CAAACCCCAA AACAGACCCC 
CGAAAACTCA ACACCTTCTT TGGCGTCATG GTGCCCACCA TTCTCTCCAT GTTCAGCATT 
GTGCTCTTTC TTCGTACAGG TTTTGTTGTG GGACATGCTG GTCTTCTGCA TGGTCTCCTC 
ATGCTGTTTG TGGCTTACTT CATCATCTCA TTGACAATCC TGTCTATATG TGCCATTTCA 
ACTAATGGGG CTGTGGAAGG AGGTGGAGCT TATTTCATGA TCAGTCGTTC TCTGGGTCCA 
GAGTTTGGCG GGAGCATCGG CCTGATGTTT TACCTTGCGA AGGTGTGTGC GTGTGGTGTG 
TATGTTCTCG GGCTGGTGGA GGCCATAATG GACGTCTTCG GTCAGGATCC AGGCTCTTCT 
GTGGCACAGG GGCTGCGTGT GTTGCCGCAG GGCTACTGGT ACACCGTGTT GTACTCCTCT 
GTAGTGTTGT TGCTGTGTAT GCTGGTGTGT CTGGTCGGTG CTCACATCTA CGCCAAAGCC 
TCTTTTCTCA TTCTGCTGGT GGTGACGGTG TCTCTGATCT CCATTATCAT CAGCCCACTG 
ATAGTCAGTC CACAGGGCTT TAACATCACG CACACGTACG GCAACAACCA CAGTGTTACG 
GTCAGTCCAA GCTACACGGG CTTTAACAGC ACCACTCTGA AGAACAACCT TGGACCTCGC 
TACTCTTTGG ACTACAGTAC CAACACAATG ATGTCTTTCG CCACTGTGTT TGCTGTGATG 
TTCACCAGCT GCACAGGCAT CATGGCAGGA GCCAACATGT CGGGTGAGCT CAAGAATCCC 
AGTGAATCAA TACCTAAAGG GACCATTATG GCGGTGGCGT ACACCTTCAC TGTCTATGTG 
CTTCTCTATC TCCTGCTGAG CTCTACCTGT GACAGGTCGT TGTTACTCAA TGATTACGCA 
GTGTTTCAGA GAGTGAACGT GTGGCCTCCA TTCGTGACCA TCGGCGTTTA CTGCGCTTCT 
CTCTCAGCTG CCATGTGCTC TATGATAGGA GCCTCTCGAA TCCTCCATGC TCTTGCCCTG 
GACCAACTCT TTGGTCTTCC TTTGGCTCCA GCTGCTGTCA CCTCCAGTTC GGGGAACCCA 
TGGGTCTCTG TGCTCTACAC CTGGGCTCTG GTGCAGTGTA CGCTGTTTGC AGGGCAGCTG 
AATGTGATTG CTGGTATTGT GACAGTGTTT TATCTGTTGG CGTATGCTGC GGTTGATTTG 
GCCTGTCTTG CTCTGGAATG GGCATCTGCT CCAAACTTCA GGCCGACGTT CCAGTTTTTC 
TCCTGGCACA CGTGTCTGTT GGGTATCATC AGCTGTGTGG TGATGATGTT CGTGATTAAC 
CCTGTGTATT CCTCAGCCAG TATTGTGCTG CTGCTGCTCC TGCTGCTCTT CCTGCATTAC 
CGATCTCCCA CCAGCAGCTG GGGCTACATC AGCCAAGCCC TCATCTTCCA TCAGGTGAGG 
AAGTACCTTC TGATGTTGGA CTCTCGGAAG GATCATGTGA AGTTCTGGCG GCCGCAGGTG 
TTACTGATGG TGTCCAACCC ACGCTCCTCC TGTCAGCTCA TCTGCTTTGT CAACCAGCTG 
AAGAAAGGGG GCCTGTTTGT CCTTGGACAC GTGCAGATTG GAGATCTGGA TGTGCTGCCA 
GCAGACCCGG TGCAGCCGCA GTATAATTTC TGGTTGAGTC TGGTGGATAA GCTGGGTGTG 
AAGGCCTTTG TGGATCTGAC TCTTTCTCCC TCGGTCAGGC AGGGCACTCA ACATCTGCTC 
CGCATCACTG GACTTGGAGG AATGAAGCCT AATACCCTGG TGTTGGGCTT TTATGACAAC 
TCCTATCCAG AGGACTACTT CCTCCAGGAC CCTGTCTTTT GCAAGGGCGA CAGATCTGAA 
GGGGATAATT TCGGTGTGGA CCTCCCATCC CTCCAGGCTC ATTTTCCACC GGTGCGCCAT 
GCCGAGAGCC CTCGCGCACT GCAGCCTCAG GAGTACGTCA GCATCATTCA GGATGCCATC 
AAAATGGGCA AGAACATTTG CTTGGCGCGC TACTTCTTCC AGCTGCCGCC CGAGAGCAAA 
GGTGCAACGT ACATGTGGGG AAAAGACAGC ATGGACACCA TCGACGTGTG GCCGACAAAC 
CTCTTGACTC CAGGTAGTGC CAGTTACGCT GACGTGGGCA GTCTGTTCCT GCTTCAGATG 
GCGTGTGTGT TGAACATGGC CAGCGGGTGG CGCCGTGCGA GATTGCGTAT ATTCGTGTGT 
GTGGAGTCTG AATCGGAAGA TCAGGGCTGG CTGGCCAAAG AGGAGCAGTT TAGAGAGTTG 
CTGGGAAAGC TGAGGATTCG TGCTGCTATT AAGATCGTGG CGTGGGATAA TGTCGCACGC 
ATGGTTAGAG GGCCAAACAC AGAGAGCCAA CCGGTGTCAG AAGACTTCCT CTGTGCTGTG 
AATGGCCTTC TGAAGGAGCA CAGCTCGACT GCCGCCGTGC GCTTTCTGTA TTTGCCCGAT 
CCCCCATCTA GTTGTGAGCT GTCCCAACAG TACCTCACCC AGCTAGACAC ACTGACCCGG 
GATCTGGGCC CGACGCTCTT GATTCATGGG GTTACCCCAG TCACGTGCAC TGAGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021331993.1
CDS	760..3459
Translation	MANEHSPLLVHGVYSMMGNAEDSRGGSAGTGEASNPKTDPRKLNTFFGVMVPTILSMFSIVLFLRTGFVVGHAGLLHGLLMLFVAYFIISLTILSICAISTNGAVEGGGAYFMISRSLGPEFGGSIGLMFYLAKVCACGVYVLGLVEAIMDVFGQDPGSSVAQGLRVLPQGYWYTVLYSSVVLLLCMLVCLVGAHIYAKASFLILLVVTVSLISIIISPLIVSPQGFNITHTYGNNHSVTVSPSYTGFNSTTLKNNLGPRYSLDYSTNTMMSFATVFAVMFTSCTGIMAGANMSGELKNPSESIPKGTIMAVAYTFTVYVLLYLLLSSTCDRSLLLNDYAVFQRVNVWPPFVTIGVYCASLSAAMCSMIGASRILHALALDQLFGLPLAPAAVTSSSGNPWVSVLYTWALVQCTLFAGQLNVIAGIVTVFYLLAYAAVDLACLALEWASAPNFRPTFQFFSWHTCLLGIISCVVMMFVINPVYSSASIVLLLLLLLFLHYRSPTSSWGYISQALIFHQVRKYLLMLDSRKDHVKFWRPQVLLMVSNPRSSCQLICFVNQLKKGGLFVLGHVQIGDLDVLPADPVQPQYNFWLSLVDKLGVKAFVDLTLSPSVRQGTQHLLRITGLGGMKPNTLVLGFYDNSYPEDYFLQDPVFCKGDRSEGDNFGVDLPSLQAHFPPVRHAESPRALQPQEYVSIIQDAIKMGKNICLARYFFQLPPESKGATYMWGKDSMDTIDVWPTNLLTPGSASYADVGSLFLLQMACVLNMASGWRRARLRIFVCVESESEDQGWLAKEEQFRELLGKLRIRAAIKIVAWDNVARMVRGPNTESQPVSEDFLCAVNGLLKEHSSTAAVRFLYLPDPPSSCELSQQYLTQLDTLTRDLGPTLLIHGVTPVTCTEL

Target ORF information:

RefSeq Version	XM_021476318.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio solute carrier family 12, member 9 (slc12a9), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021476318.1

ORF Insert Sequence:

ATGGCAAATG AGCACTCGCC TCTTCTGGTT CATGGGGTTT ACAGCATGAT GGGAAATGCT 
GAAGACTCCA GGGGTGGATC AGCAGGAACA GGAGAGGCGT CAAACCCCAA AACAGACCCC 
CGAAAACTCA ACACCTTCTT TGGCGTCATG GTGCCCACCA TTCTCTCCAT GTTCAGCATT 
GTGCTCTTTC TTCGTACAGG TTTTGTTGTG GGACATGCTG GTCTTCTGCA TGGTCTCCTC 
ATGCTGTTTG TGGCTTACTT CATCATCTCA TTGACAATCC TGTCTATATG TGCCATTTCA 
ACTAATGGGG CTGTGGAAGG AGGTGGAGCT TATTTCATGA TCAGTCGTTC TCTGGGTCCA 
GAGTTTGGCG GGAGCATCGG CCTGATGTTT TACCTTGCGA AGGTGTGTGC GTGTGGTGTG 
TATGTTCTCG GGCTGGTGGA GGCCATAATG GACGTCTTCG GTCAGGATCC AGGCTCTTCT 
GTGGCACAGG GGCTGCGTGT GTTGCCGCAG GGCTACTGGT ACACCGTGTT GTACTCCTCT 
GTAGTGTTGT TGCTGTGTAT GCTGGTGTGT CTGGTCGGTG CTCACATCTA CGCCAAAGCC 
TCTTTTCTCA TTCTGCTGGT GGTGACGGTG TCTCTGATCT CCATTATCAT CAGCCCACTG 
ATAGTCAGTC CACAGGGCTT TAACATCACG CACACGTACG GCAACAACCA CAGTGTTACG 
GTCAGTCCAA GCTACACGGG CTTTAACAGC ACCACTCTGA AGAACAACCT TGGACCTCGC 
TACTCTTTGG ACTACAGTAC CAACACAATG ATGTCTTTCG CCACTGTGTT TGCTGTGATG 
TTCACCAGCT GCACAGGCAT CATGGCAGGA GCCAACATGT CGGGTGAGCT CAAGAATCCC 
AGTGAATCAA TACCTAAAGG GACCATTATG GCGGTGGCGT ACACCTTCAC TGTCTATGTG 
CTTCTCTATC TCCTGCTGAG CTCTACCTGT GACAGGTCGT TGTTACTCAA TGATTACGCA 
GTGTTTCAGA GAGTGAACGT GTGGCCTCCA TTCGTGACCA TCGGCGTTTA CTGCGCTTCT 
CTCTCAGCTG CCATGTGCTC TATGATAGGA GCCTCTCGAA TCCTCCATGC TCTTGCCCTG 
GACCAACTCT TTGGTCTTCC TTTGGCTCCA GCTGCTGTCA CCTCCAGTTC GGGGAACCCA 
TGGGTCTCTG TGCTCTACAC CTGGGCTCTG GTGCAGTGTA CGCTGTTTGC AGGGCAGCTG 
AATGTGATTG CTGGTATTGT GACAGTGTTT TATCTGTTGG CGTATGCTGC GGTTGATTTG 
GCCTGTCTTG CTCTGGAATG GGCATCTGCT CCAAACTTCA GGCCGACGTT CCAGTTTTTC 
TCCTGGCACA CGTGTCTGTT GGGTATCATC AGCTGTGTGG TGATGATGTT CGTGATTAAC 
CCTGTGTATT CCTCAGCCAG TATTGTGCTG CTGCTGCTCC TGCTGCTCTT CCTGCATTAC 
CGATCTCCCA CCAGCAGCTG GGGCTACATC AGCCAAGCCC TCATCTTCCA TCAGGTGAGG 
AAGTACCTTC TGATGTTGGA CTCTCGGAAG GATCATGTGA AGTTCTGGCG GCCGCAGGTG 
TTACTGATGG TGTCCAACCC ACGCTCCTCC TGTCAGCTCA TCTGCTTTGT CAACCAGCTG 
AAGAAAGGGG GCCTGTTTGT CCTTGGACAC GTGCAGATTG GAGATCTGGA TGTGCTGCCA 
GCAGACCCGG TGCAGCCGCA GTATAATTTC TGGTTGAGTC TGGTGGATAA GCTGGGTGTG 
AAGGCCTTTG TGGATCTGAC TCTTTCTCCC TCGGTCAGGC AGGGCACTCA ACATCTGCTC 
CGCATCACTG GACTTGGAGG AATGAAGCCT AATACCCTGG TGTTGGGCTT TTATGACAAC 
TCCTATCCAG AGGACTACTT CCTCCAGGAC CCTGTCTTTT GCAAGGGCGA CAGATCTGAA 
GGGGATAATT TCGGTGTGGA CCTCCCATCC CTCCAGGCTC ATTTTCCACC GGTGCGCCAT 
GCCGAGAGCC CTCGCGCACT GCAGCCTCAG GAGTACGTCA GCATCATTCA GGATGCCATC 
AAAATGGGCA AGAACATTTG CTTGGCGCGC TACTTCTTCC AGCTGCCGCC CGAGAGCAAA 
GGTGCAACGT ACATGTGGGG AAAAGACAGC ATGGACACCA TCGACGTGTG GCCGACAAAC 
CTCTTGACTC CAGGTAGTGC CAGTTACGCT GACGTGGGCA GTCTGTTCCT GCTTCAGATG 
GCGTGTGTGT TGAACATGGC CAGCGGGTGG CGCCGTGCGA GATTGCGTAT ATTCGTGTGT 
GTGGAGTCTG AATCGGAAGA TCAGGGCTGG CTGGCCAAAG AGGAGCAGTT TAGAGAGTTG 
CTGGGAAAGC TGAGGATTCG TGCTGCTATT AAGATCGTGG CGTGGGATAA TGTCGCACGC 
ATGGTTAGAG GGCCAAACAC AGAGAGCCAA CCGGTGTCAG AAGACTTCCT CTGTGCTGTG 
AATGGCCTTC TGAAGGAGCA CAGCTCGACT GCCGCCGTGC GCTTTCTGTA TTTGCCCGAT 
CCCCCATCTA GTTGTGAGCT GTCCCAACAG TACCTCACCC AGCTAGACAC ACTGACCCGG 
GATCTGGGCC CGACGCTCTT GATTCATGGG GTTACCCCAG TCACGTGCAC TGAGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SLC12A9 ( XM_005165365.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005165422.1 Danio rerio solute carrier family 12, member 9 (slc12a9), transcript variant X1, mRNA.

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CloneID	ODa03788
Clone ID Related Accession (Same CDS sequence)	XM_005165365.4 , NM_001128548.1 , XM_017356172.1 , XM_021476318.1 , XM_005165365.3
Accession Version	XM_005165365.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2700bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	solute carrier family 12 member 9 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005165365.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAATG AGCACTCGCC TCTTCTGGTT CATGGGGTTT ACAGCATGAT GGGAAATGCT 
GAAGACTCCA GGGGTGGATC AGCAGGAACA GGAGAGGCGT CAAACCCCAA AACAGACCCC 
CGAAAACTCA ACACCTTCTT TGGCGTCATG GTGCCCACCA TTCTCTCCAT GTTCAGCATT 
GTGCTCTTTC TTCGTACAGG TTTTGTTGTG GGACATGCTG GTCTTCTGCA TGGTCTCCTC 
ATGCTGTTTG TGGCTTACTT CATCATCTCA TTGACAATCC TGTCTATATG TGCCATTTCA 
ACTAATGGGG CTGTGGAAGG AGGTGGAGCT TATTTCATGA TCAGTCGTTC TCTGGGTCCA 
GAGTTTGGCG GGAGCATCGG CCTGATGTTT TACCTTGCGA AGGTGTGTGC GTGTGGTGTG 
TATGTTCTCG GGCTGGTGGA GGCCATAATG GACGTCTTCG GTCAGGATCC AGGCTCTTCT 
GTGGCACAGG GGCTGCGTGT GTTGCCGCAG GGCTACTGGT ACACCGTGTT GTACTCCTCT 
GTAGTGTTGT TGCTGTGTAT GCTGGTGTGT CTGGTCGGTG CTCACATCTA CGCCAAAGCC 
TCTTTTCTCA TTCTGCTGGT GGTGACGGTG TCTCTGATCT CCATTATCAT CAGCCCACTG 
ATAGTCAGTC CACAGGGCTT TAACATCACG CACACGTACG GCAACAACCA CAGTGTTACG 
GTCAGTCCAA GCTACACGGG CTTTAACAGC ACCACTCTGA AGAACAACCT TGGACCTCGC 
TACTCTTTGG ACTACAGTAC CAACACAATG ATGTCTTTCG CCACTGTGTT TGCTGTGATG 
TTCACCAGCT GCACAGGCAT CATGGCAGGA GCCAACATGT CGGGTGAGCT CAAGAATCCC 
AGTGAATCAA TACCTAAAGG GACCATTATG GCGGTGGCGT ACACCTTCAC TGTCTATGTG 
CTTCTCTATC TCCTGCTGAG CTCTACCTGT GACAGGTCGT TGTTACTCAA TGATTACGCA 
GTGTTTCAGA GAGTGAACGT GTGGCCTCCA TTCGTGACCA TCGGCGTTTA CTGCGCTTCT 
CTCTCAGCTG CCATGTGCTC TATGATAGGA GCCTCTCGAA TCCTCCATGC TCTTGCCCTG 
GACCAACTCT TTGGTCTTCC TTTGGCTCCA GCTGCTGTCA CCTCCAGTTC GGGGAACCCA 
TGGGTCTCTG TGCTCTACAC CTGGGCTCTG GTGCAGTGTA CGCTGTTTGC AGGGCAGCTG 
AATGTGATTG CTGGTATTGT GACAGTGTTT TATCTGTTGG CGTATGCTGC GGTTGATTTG 
GCCTGTCTTG CTCTGGAATG GGCATCTGCT CCAAACTTCA GGCCGACGTT CCAGTTTTTC 
TCCTGGCACA CGTGTCTGTT GGGTATCATC AGCTGTGTGG TGATGATGTT CGTGATTAAC 
CCTGTGTATT CCTCAGCCAG TATTGTGCTG CTGCTGCTCC TGCTGCTCTT CCTGCATTAC 
CGATCTCCCA CCAGCAGCTG GGGCTACATC AGCCAAGCCC TCATCTTCCA TCAGGTGAGG 
AAGTACCTTC TGATGTTGGA CTCTCGGAAG GATCATGTGA AGTTCTGGCG GCCGCAGGTG 
TTACTGATGG TGTCCAACCC ACGCTCCTCC TGTCAGCTCA TCTGCTTTGT CAACCAGCTG 
AAGAAAGGGG GCCTGTTTGT CCTTGGACAC GTGCAGATTG GAGATCTGGA TGTGCTGCCA 
GCAGACCCGG TGCAGCCGCA GTATAATTTC TGGTTGAGTC TGGTGGATAA GCTGGGTGTG 
AAGGCCTTTG TGGATCTGAC TCTTTCTCCC TCGGTCAGGC AGGGCACTCA ACATCTGCTC 
CGCATCACTG GACTTGGAGG AATGAAGCCT AATACCCTGG TGTTGGGCTT TTATGACAAC 
TCCTATCCAG AGGACTACTT CCTCCAGGAC CCTGTCTTTT GCAAGGGCGA CAGATCTGAA 
GGGGATAATT TCGGTGTGGA CCTCCCATCC CTCCAGGCTC ATTTTCCACC GGTGCGCCAT 
GCCGAGAGCC CTCGCGCACT GCAGCCTCAG GAGTACGTCA GCATCATTCA GGATGCCATC 
AAAATGGGCA AGAACATTTG CTTGGCGCGC TACTTCTTCC AGCTGCCGCC CGAGAGCAAA 
GGTGCAACGT ACATGTGGGG AAAAGACAGC ATGGACACCA TCGACGTGTG GCCGACAAAC 
CTCTTGACTC CAGGTAGTGC CAGTTACGCT GACGTGGGCA GTCTGTTCCT GCTTCAGATG 
GCGTGTGTGT TGAACATGGC CAGCGGGTGG CGCCGTGCGA GATTGCGTAT ATTCGTGTGT 
GTGGAGTCTG AATCGGAAGA TCAGGGCTGG CTGGCCAAAG AGGAGCAGTT TAGAGAGTTG 
CTGGGAAAGC TGAGGATTCG TGCTGCTATT AAGATCGTGG CGTGGGATAA TGTCGCACGC 
ATGGTTAGAG GGCCAAACAC AGAGAGCCAA CCGGTGTCAG AAGACTTCCT CTGTGCTGTG 
AATGGCCTTC TGAAGGAGCA CAGCTCGACT GCCGCCGTGC GCTTTCTGTA TTTGCCCGAT 
CCCCCATCTA GTTGTGAGCT GTCCCAACAG TACCTCACCC AGCTAGACAC ACTGACCCGG 
GATCTGGGCC CGACGCTCTT GATTCATGGG GTTACCCCAG TCACGTGCAC TGAGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005165422.1
CDS	1176..3875
Translation	MANEHSPLLVHGVYSMMGNAEDSRGGSAGTGEASNPKTDPRKLNTFFGVMVPTILSMFSIVLFLRTGFVVGHAGLLHGLLMLFVAYFIISLTILSICAISTNGAVEGGGAYFMISRSLGPEFGGSIGLMFYLAKVCACGVYVLGLVEAIMDVFGQDPGSSVAQGLRVLPQGYWYTVLYSSVVLLLCMLVCLVGAHIYAKASFLILLVVTVSLISIIISPLIVSPQGFNITHTYGNNHSVTVSPSYTGFNSTTLKNNLGPRYSLDYSTNTMMSFATVFAVMFTSCTGIMAGANMSGELKNPSESIPKGTIMAVAYTFTVYVLLYLLLSSTCDRSLLLNDYAVFQRVNVWPPFVTIGVYCASLSAAMCSMIGASRILHALALDQLFGLPLAPAAVTSSSGNPWVSVLYTWALVQCTLFAGQLNVIAGIVTVFYLLAYAAVDLACLALEWASAPNFRPTFQFFSWHTCLLGIISCVVMMFVINPVYSSASIVLLLLLLLFLHYRSPTSSWGYISQALIFHQVRKYLLMLDSRKDHVKFWRPQVLLMVSNPRSSCQLICFVNQLKKGGLFVLGHVQIGDLDVLPADPVQPQYNFWLSLVDKLGVKAFVDLTLSPSVRQGTQHLLRITGLGGMKPNTLVLGFYDNSYPEDYFLQDPVFCKGDRSEGDNFGVDLPSLQAHFPPVRHAESPRALQPQEYVSIIQDAIKMGKNICLARYFFQLPPESKGATYMWGKDSMDTIDVWPTNLLTPGSASYADVGSLFLLQMACVLNMASGWRRARLRIFVCVESESEDQGWLAKEEQFRELLGKLRIRAAIKIVAWDNVARMVRGPNTESQPVSEDFLCAVNGLLKEHSSTAAVRFLYLPDPPSSCELSQQYLTQLDTLTRDLGPTLLIHGVTPVTCTEL

Target ORF information:

RefSeq Version	XM_005165365.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio solute carrier family 12, member 9 (slc12a9), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005165365.3

ORF Insert Sequence:

ATGGCAAATG AGCACTCGCC TCTTCTGGTT CATGGGGTTT ACAGCATGAT GGGAAATGCT 
GAAGACTCCA GGGGTGGATC AGCAGGAACA GGAGAGGCGT CAAACCCCAA AACAGACCCC 
CGAAAACTCA ACACCTTCTT TGGCGTCATG GTGCCCACCA TTCTCTCCAT GTTCAGCATT 
GTGCTCTTTC TTCGTACAGG TTTTGTTGTG GGACATGCTG GTCTTCTGCA TGGTCTCCTC 
ATGCTGTTTG TGGCTTACTT CATCATCTCA TTGACAATCC TGTCTATATG TGCCATTTCA 
ACTAATGGGG CTGTGGAAGG AGGTGGAGCT TATTTCATGA TCAGTCGTTC TCTGGGTCCA 
GAGTTTGGCG GGAGCATCGG CCTGATGTTT TACCTTGCGA AGGTGTGTGC GTGTGGTGTG 
TATGTTCTCG GGCTGGTGGA GGCCATAATG GACGTCTTCG GTCAGGATCC AGGCTCTTCT 
GTGGCACAGG GGCTGCGTGT GTTGCCGCAG GGCTACTGGT ACACCGTGTT GTACTCCTCT 
GTAGTGTTGT TGCTGTGTAT GCTGGTGTGT CTGGTCGGTG CTCACATCTA CGCCAAAGCC 
TCTTTTCTCA TTCTGCTGGT GGTGACGGTG TCTCTGATCT CCATTATCAT CAGCCCACTG 
ATAGTCAGTC CACAGGGCTT TAACATCACG CACACGTACG GCAACAACCA CAGTGTTACG 
GTCAGTCCAA GCTACACGGG CTTTAACAGC ACCACTCTGA AGAACAACCT TGGACCTCGC 
TACTCTTTGG ACTACAGTAC CAACACAATG ATGTCTTTCG CCACTGTGTT TGCTGTGATG 
TTCACCAGCT GCACAGGCAT CATGGCAGGA GCCAACATGT CGGGTGAGCT CAAGAATCCC 
AGTGAATCAA TACCTAAAGG GACCATTATG GCGGTGGCGT ACACCTTCAC TGTCTATGTG 
CTTCTCTATC TCCTGCTGAG CTCTACCTGT GACAGGTCGT TGTTACTCAA TGATTACGCA 
GTGTTTCAGA GAGTGAACGT GTGGCCTCCA TTCGTGACCA TCGGCGTTTA CTGCGCTTCT 
CTCTCAGCTG CCATGTGCTC TATGATAGGA GCCTCTCGAA TCCTCCATGC TCTTGCCCTG 
GACCAACTCT TTGGTCTTCC TTTGGCTCCA GCTGCTGTCA CCTCCAGTTC GGGGAACCCA 
TGGGTCTCTG TGCTCTACAC CTGGGCTCTG GTGCAGTGTA CGCTGTTTGC AGGGCAGCTG 
AATGTGATTG CTGGTATTGT GACAGTGTTT TATCTGTTGG CGTATGCTGC GGTTGATTTG 
GCCTGTCTTG CTCTGGAATG GGCATCTGCT CCAAACTTCA GGCCGACGTT CCAGTTTTTC 
TCCTGGCACA CGTGTCTGTT GGGTATCATC AGCTGTGTGG TGATGATGTT CGTGATTAAC 
CCTGTGTATT CCTCAGCCAG TATTGTGCTG CTGCTGCTCC TGCTGCTCTT CCTGCATTAC 
CGATCTCCCA CCAGCAGCTG GGGCTACATC AGCCAAGCCC TCATCTTCCA TCAGGTGAGG 
AAGTACCTTC TGATGTTGGA CTCTCGGAAG GATCATGTGA AGTTCTGGCG GCCGCAGGTG 
TTACTGATGG TGTCCAACCC ACGCTCCTCC TGTCAGCTCA TCTGCTTTGT CAACCAGCTG 
AAGAAAGGGG GCCTGTTTGT CCTTGGACAC GTGCAGATTG GAGATCTGGA TGTGCTGCCA 
GCAGACCCGG TGCAGCCGCA GTATAATTTC TGGTTGAGTC TGGTGGATAA GCTGGGTGTG 
AAGGCCTTTG TGGATCTGAC TCTTTCTCCC TCGGTCAGGC AGGGCACTCA ACATCTGCTC 
CGCATCACTG GACTTGGAGG AATGAAGCCT AATACCCTGG TGTTGGGCTT TTATGACAAC 
TCCTATCCAG AGGACTACTT CCTCCAGGAC CCTGTCTTTT GCAAGGGCGA CAGATCTGAA 
GGGGATAATT TCGGTGTGGA CCTCCCATCC CTCCAGGCTC ATTTTCCACC GGTGCGCCAT 
GCCGAGAGCC CTCGCGCACT GCAGCCTCAG GAGTACGTCA GCATCATTCA GGATGCCATC 
AAAATGGGCA AGAACATTTG CTTGGCGCGC TACTTCTTCC AGCTGCCGCC CGAGAGCAAA 
GGTGCAACGT ACATGTGGGG AAAAGACAGC ATGGACACCA TCGACGTGTG GCCGACAAAC 
CTCTTGACTC CAGGTAGTGC CAGTTACGCT GACGTGGGCA GTCTGTTCCT GCTTCAGATG 
GCGTGTGTGT TGAACATGGC CAGCGGGTGG CGCCGTGCGA GATTGCGTAT ATTCGTGTGT 
GTGGAGTCTG AATCGGAAGA TCAGGGCTGG CTGGCCAAAG AGGAGCAGTT TAGAGAGTTG 
CTGGGAAAGC TGAGGATTCG TGCTGCTATT AAGATCGTGG CGTGGGATAA TGTCGCACGC 
ATGGTTAGAG GGCCAAACAC AGAGAGCCAA CCGGTGTCAG AAGACTTCCT CTGTGCTGTG 
AATGGCCTTC TGAAGGAGCA CAGCTCGACT GCCGCCGTGC GCTTTCTGTA TTTGCCCGAT 
CCCCCATCTA GTTGTGAGCT GTCCCAACAG TACCTCACCC AGCTAGACAC ACTGACCCGG 
GATCTGGGCC CGACGCTCTT GATTCATGGG GTTACCCCAG TCACGTGCAC TGAGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Expression and regulation of drug transporters in vertebrate neutrophils.
Scientific reports7(1)4967(2017 Jul)
Foulkes MJ,Henry KM,Rougeot J,Hooper-Greenhill E,Loynes CA,Jeffrey P,Fleming A,Savage CO,Meijer AH,Jones S,Renshaw SA

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 Mar)
Bay?s ?,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

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SLC12A9 cDNA ORF clone, Danio rerio(Zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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