aars2 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	aars2
Entrez Gene ID	792787
Full Name	alanyl-tRNA synthetase 2, mitochondrial (putative)
Synonyms	im:7141191,si:dkey-240e12.1
General protein information	Preferred Names alanyl-tRNA synthetase 2, mitochondrial (putative) Names probable alanyl-tRNA synthetase, mitochondrial alanine--tRNA ligase, mitochondrial
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021473512.1	XP_021329187.1	alanine--tRNA ligase, mitochondrial isoform X1
XM_001332352.8	XP_001332388.5	alanine--tRNA ligase, mitochondrial isoform X1
XM_001332352.7	XP_001332388.5	alanine--tRNA ligase, mitochondrial isoform X1
XM_009296409.2	XP_009294684.1	alanine--tRNA ligase, mitochondrial isoform X2
XM_009296409.3	XP_009294684.1	alanine--tRNA ligase, mitochondrial isoform X2

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	AARS2	XP_518510.2
Mus musculus (house mouse)	Aars2	NP_941010.2
Homo sapiens (human)	AARS2	NP_065796.1
Rattus norvegicus (Norway rat)	Aars2	NP_001100361.1
Gallus gallus (chicken)	AARS2	NP_001026227.1
	AARS2	XP_001099647.1
Canis lupus familiaris (dog)	AARS2	XP_532155.2
Danio rerio (zebrafish)	aars2	XP_001332388.5
Bos taurus (cattle)	AARS2	NP_001178140.1

Gene Ontology

The following aars2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the aars2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa46717	XM_009296409.3 Latest version!	Danio rerio alanyl-tRNA synthetase 2, mitochondrial (putative) (aars2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
ODa146178	XM_021473512.1 Latest version!	Danio rerio alanyl-tRNA synthetase 2, mitochondrial (putative) (aars2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
ODa27040	XM_001332352.8 Latest version!	Danio rerio alanyl-tRNA synthetase 2, mitochondrial (putative) (aars2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
View Old Accession Versions >>
ODa27040	XM_001332352.7	Danio rerio alanyl-tRNA synthetase 2, mitochondrial (putative) (aars2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa46717	XM_009296409.2	Danio rerio alanyl-tRNA synthetase 2, mitochondrial (putative) (aars2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

aars2 ( XM_009296409.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009294684.1 Danio rerio alanyl-tRNA synthetase 2, mitochondrial (putative) (aars2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa46717
Clone ID Related Accession (Same CDS sequence)	XM_009296409.2 , XM_009296409.3
Accession Version	XM_009296409.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2571bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	alanine--tRNA ligase, mitochondrial isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007133.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009296409.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGCGC GCATTACAGT CGTGAAACAT GTTCACTGTG CACTTCTAAT TACAAAATTG 
AGCTGTTTGT CTTCGGGAAT AAGAAGATGC ACGGCTCTGT CATTCTCAGT ATATCGTAGG 
GAATTCACAT CTAAAAGAGT ACGGCGGAAG TTTATCGACT TTTTCAGGGA AGGTTATGGA 
CATCGGGTGG TTCCGTCATC CTCCGTACGC CCGAGAGGAG ACCCCAGTCT GCTATTCGTC 
AATGCAGGAA TGAACCAGTT TAAGCCGATT CTGCTGGGCT GTGCTGACCC TCGCAGTGAG 
ATGGTCTCGT ACCGGCGGGT GGTAAACAGT CAGAAGTGTG TCCGTGCTGG AGGCAAACAC 
AATGACCTAC AGGATGTGGG GAAGGACGTT TACCATCATA CCTTCTTCGA GATGCTGGGG 
AACTGGTCAT TCGGGGACTA CTTCAAGGTG GAAGCTTGTG CAATGGCCTG GCGTCTTTTA 
ACGGAAGAGT ATGGCATTCC TGCGCAGCGC CTGTATGTCT CGTACTTTTC TGGAGATCCA 
GCTAATGGTC TACCTGCTGA TGAGGAGACA CGGGACATCT GGCTCAGCAT GGGACTGAGA 
GCGGATCATG TGTTGCCATT TGGGATGAAG GATAACTTCT GGGAAATGGG AGAGACGGGC 
CCATGTGGCC CCTGCACGGA GATTCACTAC GATCACATTG GCAACAGGAA CGCTGCCTTA 
CTGGTCAATG CAGACAGTCC AGATGTAGTG GAAATCTGGA ACCTGGTATT CATGCAATAT 
AACAGGGAGA GCAATGGTAG TCTGCGAGCT CTTCCCCAGT GCAGTGTGGA TACTGGCATG 
GGTTTAGAGA GGCTGGTGAC GGTGCTGCAA GGCAAACGCT CCAACTACGA CACAGATCTC 
TTCACACCTC TATTATCTGC CATCCACCAG TGCTCTAAGG CTCCCGCATA TCAGGGCAGA 
ACCGGAGCGG CTGATGTGGC TCAGCTGGAC ATGGCCTATC GGGTCGTGGC GGATCACATC 
CGAACAATCT GTGTGTGTAT CGCTGACGGA GTTTACCCAG GCATGGCAGG CGCTGAGCTG 
GTGTTAAGAC GCATCTTAAG ACGTGCGGTT AGGTTTTCTA CAGAGGTGCT GCAGGCTCCT 
GAGGGTGCGC TGGCAAGTCT GGTGCCCACT GTAGCTCATC TACTGGGTGA TGCCTATCCT 
GAGCTGAACA CAGAGTCTGA TAGAATCATG GATCTCATTA ATCAGAATGA AGCCCACTTC 
CTTTCCTCGC TGAAACAGGG TAGGAGGGTG ATTGACAGGA CTCTCAGCAA CATGGACGAA 
AACTCTTCTT TTTTTCCAGC CTCGGTTGCC TGGTCCTTAT ATCGCAACCT GGGCTTTCCT 
CTGGATCTCA TTGACCTAAT GCTAGAGGAA AAAGGCAAGA TTGTTGATAA AAAGGAAATG 
AAGGTGCTGG AAGATGAATA TGAGAAGTTG GGTCTTCAGT CAGAGGAGGA TGAAGGTGAA 
AAGCTGGACC AGCTGGACCT TCATAGCCTG GCGGAGCTAC AACGGCAAAG AGTTCCTCAC 
ACAGACGACT CTCCAAAATA CTGTTACAGT GTGGGGTCCG ATGGACAATA CGTGTTTGAG 
CCCTGCAAAG CGTCTGTCCT GGCACTTTAC TGTGATGGGG CACTGGTTTC AGAGGTGCGT 
CAGGGTCAGC ACTGCGCTGT GATCCTGGAC CAGACCAGCT TTTATGCAGA GCAGGGCGGA 
CAGGCCAATG ACCAGGGTTA CTTCACCAAG GATGGGCTGC AGGATGTGTT GTTCCCTGTA 
AAGTGTGTCC GTCTCGCTGG GGGTTATGTG GTACACAAAG TCACAGCAGC AGAGACTTTG 
AGAACTGGAG ATCAAGTGCA TCTTCACGTG GATGAGGCTT GGCGGCTGGG CTGCATGATA 
AATCACACCG CCACCCATGT GCTGAACTTT GCTCTGCGAG AGTTGCTAGG TCCCATTGTC 
AGCCAACGAG GGTCTCATTG CAGTGCGAAT CGTCTACGCT TCGACTTCAG TGTCAAGGGC 
ACCCTGACTG TCTCTGAGCT GCAGAAAGTT GAGGAGCTGG TGCTCAATAT CATTCGTCAA 
AATGCAGATG TGCACGTAGA GGAGGTTCCT CTGTCGAGTG CTAAACAAAT TGCTGGCCTG 
AGGACTGTAG ATGAGCTCTA TCCAGACCCA GTGCGAGTGG TGTCCGTGGC TGTGCCTGTT 
TCGGATCTGC TTGCTTCAGA AAGAGCTGAG CAAACTTCAG TTGAGCTCTG CTGTGGCACG 
CATTTACTGA GGACGGGAGC AATTAGGGAC TTTGTTGTTG TATCTGAGCG ACAGATGATG 
AAAGGCGTTT GCCGTATTGT TGCCTTTACT GGAGATGACG CCATAAAGGC TAGAGAGGCA 
GGCCAAGCCT TGCAGGAGGA GTTGGAGTCT CTGGAGGCCC GGATGGGATT GTCAAACACA 
TTGTCTATTC AAGAAGCAAA GCGGCTTTCC AAAGAGGTTG GCCACCTAAT CAATAAAACA 
AAGAAGATAT TTCGAAGAAA GCTTGAAAAC GCTGACGTCC ATGGCAGTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009294684.1
CDS	7..2577
Translation	MAARITVVKHVHCALLITKLSCLSSGIRRCTALSFSVYRREFTSKRVRRKFIDFFREGYGHRVVPSSSVRPRGDPSLLFVNAGMNQFKPILLGCADPRSEMVSYRRVVNSQKCVRAGGKHNDLQDVGKDVYHHTFFEMLGNWSFGDYFKVEACAMAWRLLTEEYGIPAQRLYVSYFSGDPANGLPADEETRDIWLSMGLRADHVLPFGMKDNFWEMGETGPCGPCTEIHYDHIGNRNAALLVNADSPDVVEIWNLVFMQYNRESNGSLRALPQCSVDTGMGLERLVTVLQGKRSNYDTDLFTPLLSAIHQCSKAPAYQGRTGAADVAQLDMAYRVVADHIRTICVCIADGVYPGMAGAELVLRRILRRAVRFSTEVLQAPEGALASLVPTVAHLLGDAYPELNTESDRIMDLINQNEAHFLSSLKQGRRVIDRTLSNMDENSSFFPASVAWSLYRNLGFPLDLIDLMLEEKGKIVDKKEMKVLEDEYEKLGLQSEEDEGEKLDQLDLHSLAELQRQRVPHTDDSPKYCYSVGSDGQYVFEPCKASVLALYCDGALVSEVRQGQHCAVILDQTSFYAEQGGQANDQGYFTKDGLQDVLFPVKCVRLAGGYVVHKVTAAETLRTGDQVHLHVDEAWRLGCMINHTATHVLNFALRELLGPIVSQRGSHCSANRLRFDFSVKGTLTVSELQKVEELVLNIIRQNADVHVEEVPLSSAKQIAGLRTVDELYPDPVRVVSVAVPVSDLLASERAEQTSVELCCGTHLLRTGAIRDFVVVSERQMMKGVCRIVAFTGDDAIKAREAGQALQEELESLEARMGLSNTLSIQEAKRLSKEVGHLINKTKKIFRRKLENADVHGS

Target ORF information:

RefSeq Version	XM_009296409.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio alanyl-tRNA synthetase 2, mitochondrial (putative) (aars2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009296409.2

ORF Insert Sequence:

ATGGCCGCGC GCATTACAGT CGTGAAACAT GTTCACTGTG CACTTCTAAT TACAAAATTG 
AGCTGTTTGT CTTCGGGAAT AAGAAGATGC ACGGCTCTGT CATTCTCAGT ATATCGTAGG 
GAATTCACAT CTAAAAGAGT ACGGCGGAAG TTTATCGACT TTTTCAGGGA AGGTTATGGA 
CATCGGGTGG TTCCGTCATC CTCCGTACGC CCGAGAGGAG ACCCCAGTCT GCTATTCGTC 
AATGCAGGAA TGAACCAGTT TAAGCCGATT CTGCTGGGCT GTGCTGACCC TCGCAGTGAG 
ATGGTCTCGT ACCGGCGGGT GGTAAACAGT CAGAAGTGTG TCCGTGCTGG AGGCAAACAC 
AATGACCTAC AGGATGTGGG GAAGGACGTT TACCATCATA CCTTCTTCGA GATGCTGGGG 
AACTGGTCAT TCGGGGACTA CTTCAAGGTG GAAGCTTGTG CAATGGCCTG GCGTCTTTTA 
ACGGAAGAGT ATGGCATTCC TGCGCAGCGC CTGTATGTCT CGTACTTTTC TGGAGATCCA 
GCTAATGGTC TACCTGCTGA TGAGGAGACA CGGGACATCT GGCTCAGCAT GGGACTGAGA 
GCGGATCATG TGTTGCCATT TGGGATGAAG GATAACTTCT GGGAAATGGG AGAGACGGGC 
CCATGTGGCC CCTGCACGGA GATTCACTAC GATCACATTG GCAACAGGAA CGCTGCCTTA 
CTGGTCAATG CAGACAGTCC AGATGTAGTG GAAATCTGGA ACCTGGTATT CATGCAATAT 
AACAGGGAGA GCAATGGTAG TCTGCGAGCT CTTCCCCAGT GCAGTGTGGA TACTGGCATG 
GGTTTAGAGA GGCTGGTGAC GGTGCTGCAA GGCAAACGCT CCAACTACGA CACAGATCTC 
TTCACACCTC TATTATCTGC CATCCACCAG TGCTCTAAGG CTCCCGCATA TCAGGGCAGA 
ACCGGAGCGG CTGATGTGGC TCAGCTGGAC ATGGCCTATC GGGTCGTGGC GGATCACATC 
CGAACAATCT GTGTGTGTAT CGCTGACGGA GTTTACCCAG GCATGGCAGG CGCTGAGCTG 
GTGTTAAGAC GCATCTTAAG ACGTGCGGTT AGGTTTTCTA CAGAGGTGCT GCAGGCTCCT 
GAGGGTGCGC TGGCAAGTCT GGTGCCCACT GTAGCTCATC TACTGGGTGA TGCCTATCCT 
GAGCTGAACA CAGAGTCTGA TAGAATCATG GATCTCATTA ATCAGAATGA AGCCCACTTC 
CTTTCCTCGC TGAAACAGGG TAGGAGGGTG ATTGACAGGA CTCTCAGCAA CATGGACGAA 
AACTCTTCTT TTTTTCCAGC CTCGGTTGCC TGGTCCTTAT ATCGCAACCT GGGCTTTCCT 
CTGGATCTCA TTGACCTAAT GCTAGAGGAA AAAGGCAAGA TTGTTGATAA AAAGGAAATG 
AAGGTGCTGG AAGATGAATA TGAGAAGTTG GGTCTTCAGT CAGAGGAGGA TGAAGGTGAA 
AAGCTGGACC AGCTGGACCT TCATAGCCTG GCGGAGCTAC AACGGCAAAG AGTTCCTCAC 
ACAGACGACT CTCCAAAATA CTGTTACAGT GTGGGGTCCG ATGGACAATA CGTGTTTGAG 
CCCTGCAAAG CGTCTGTCCT GGCACTTTAC TGTGATGGGG CACTGGTTTC AGAGGTGCGT 
CAGGGTCAGC ACTGCGCTGT GATCCTGGAC CAGACCAGCT TTTATGCAGA GCAGGGCGGA 
CAGGCCAATG ACCAGGGTTA CTTCACCAAG GATGGGCTGC AGGATGTGTT GTTCCCTGTA 
AAGTGTGTCC GTCTCGCTGG GGGTTATGTG GTACACAAAG TCACAGCAGC AGAGACTTTG 
AGAACTGGAG ATCAAGTGCA TCTTCACGTG GATGAGGCTT GGCGGCTGGG CTGCATGATA 
AATCACACCG CCACCCATGT GCTGAACTTT GCTCTGCGAG AGTTGCTAGG TCCCATTGTC 
AGCCAACGAG GGTCTCATTG CAGTGCGAAT CGTCTACGCT TCGACTTCAG TGTCAAGGGC 
ACCCTGACTG TCTCTGAGCT GCAGAAAGTT GAGGAGCTGG TGCTCAATAT CATTCGTCAA 
AATGCAGATG TGCACGTAGA GGAGGTTCCT CTGTCGAGTG CTAAACAAAT TGCTGGCCTG 
AGGACTGTAG ATGAGCTCTA TCCAGACCCA GTGCGAGTGG TGTCCGTGGC TGTGCCTGTT 
TCGGATCTGC TTGCTTCAGA AAGAGCTGAG CAAACTTCAG TTGAGCTCTG CTGTGGCACG 
CATTTACTGA GGACGGGAGC AATTAGGGAC TTTGTTGTTG TATCTGAGCG ACAGATGATG 
AAAGGCGTTT GCCGTATTGT TGCCTTTACT GGAGATGACG CCATAAAGGC TAGAGAGGCA 
GGCCAAGCCT TGCAGGAGGA GTTGGAGTCT CTGGAGGCCC GGATGGGATT GTCAAACACA 
TTGTCTATTC AAGAAGCAAA GCGGCTTTCC AAAGAGGTTG GCCACCTAAT CAATAAAACA 
AAGAAGATAT TTCGAAGAAA GCTTGAAAAC GCTGACGTCC ATGGCAGTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

aars2 ( XM_009296409.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009294684.1 Danio rerio alanyl-tRNA synthetase 2, mitochondrial (putative) (aars2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa46717
Clone ID Related Accession (Same CDS sequence)	XM_009296409.2 , XM_009296409.3
Accession Version	XM_009296409.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2571bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	alanine--tRNA ligase, mitochondrial isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007133.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009296409.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGCGC GCATTACAGT CGTGAAACAT GTTCACTGTG CACTTCTAAT TACAAAATTG 
AGCTGTTTGT CTTCGGGAAT AAGAAGATGC ACGGCTCTGT CATTCTCAGT ATATCGTAGG 
GAATTCACAT CTAAAAGAGT ACGGCGGAAG TTTATCGACT TTTTCAGGGA AGGTTATGGA 
CATCGGGTGG TTCCGTCATC CTCCGTACGC CCGAGAGGAG ACCCCAGTCT GCTATTCGTC 
AATGCAGGAA TGAACCAGTT TAAGCCGATT CTGCTGGGCT GTGCTGACCC TCGCAGTGAG 
ATGGTCTCGT ACCGGCGGGT GGTAAACAGT CAGAAGTGTG TCCGTGCTGG AGGCAAACAC 
AATGACCTAC AGGATGTGGG GAAGGACGTT TACCATCATA CCTTCTTCGA GATGCTGGGG 
AACTGGTCAT TCGGGGACTA CTTCAAGGTG GAAGCTTGTG CAATGGCCTG GCGTCTTTTA 
ACGGAAGAGT ATGGCATTCC TGCGCAGCGC CTGTATGTCT CGTACTTTTC TGGAGATCCA 
GCTAATGGTC TACCTGCTGA TGAGGAGACA CGGGACATCT GGCTCAGCAT GGGACTGAGA 
GCGGATCATG TGTTGCCATT TGGGATGAAG GATAACTTCT GGGAAATGGG AGAGACGGGC 
CCATGTGGCC CCTGCACGGA GATTCACTAC GATCACATTG GCAACAGGAA CGCTGCCTTA 
CTGGTCAATG CAGACAGTCC AGATGTAGTG GAAATCTGGA ACCTGGTATT CATGCAATAT 
AACAGGGAGA GCAATGGTAG TCTGCGAGCT CTTCCCCAGT GCAGTGTGGA TACTGGCATG 
GGTTTAGAGA GGCTGGTGAC GGTGCTGCAA GGCAAACGCT CCAACTACGA CACAGATCTC 
TTCACACCTC TATTATCTGC CATCCACCAG TGCTCTAAGG CTCCCGCATA TCAGGGCAGA 
ACCGGAGCGG CTGATGTGGC TCAGCTGGAC ATGGCCTATC GGGTCGTGGC GGATCACATC 
CGAACAATCT GTGTGTGTAT CGCTGACGGA GTTTACCCAG GCATGGCAGG CGCTGAGCTG 
GTGTTAAGAC GCATCTTAAG ACGTGCGGTT AGGTTTTCTA CAGAGGTGCT GCAGGCTCCT 
GAGGGTGCGC TGGCAAGTCT GGTGCCCACT GTAGCTCATC TACTGGGTGA TGCCTATCCT 
GAGCTGAACA CAGAGTCTGA TAGAATCATG GATCTCATTA ATCAGAATGA AGCCCACTTC 
CTTTCCTCGC TGAAACAGGG TAGGAGGGTG ATTGACAGGA CTCTCAGCAA CATGGACGAA 
AACTCTTCTT TTTTTCCAGC CTCGGTTGCC TGGTCCTTAT ATCGCAACCT GGGCTTTCCT 
CTGGATCTCA TTGACCTAAT GCTAGAGGAA AAAGGCAAGA TTGTTGATAA AAAGGAAATG 
AAGGTGCTGG AAGATGAATA TGAGAAGTTG GGTCTTCAGT CAGAGGAGGA TGAAGGTGAA 
AAGCTGGACC AGCTGGACCT TCATAGCCTG GCGGAGCTAC AACGGCAAAG AGTTCCTCAC 
ACAGACGACT CTCCAAAATA CTGTTACAGT GTGGGGTCCG ATGGACAATA CGTGTTTGAG 
CCCTGCAAAG CGTCTGTCCT GGCACTTTAC TGTGATGGGG CACTGGTTTC AGAGGTGCGT 
CAGGGTCAGC ACTGCGCTGT GATCCTGGAC CAGACCAGCT TTTATGCAGA GCAGGGCGGA 
CAGGCCAATG ACCAGGGTTA CTTCACCAAG GATGGGCTGC AGGATGTGTT GTTCCCTGTA 
AAGTGTGTCC GTCTCGCTGG GGGTTATGTG GTACACAAAG TCACAGCAGC AGAGACTTTG 
AGAACTGGAG ATCAAGTGCA TCTTCACGTG GATGAGGCTT GGCGGCTGGG CTGCATGATA 
AATCACACCG CCACCCATGT GCTGAACTTT GCTCTGCGAG AGTTGCTAGG TCCCATTGTC 
AGCCAACGAG GGTCTCATTG CAGTGCGAAT CGTCTACGCT TCGACTTCAG TGTCAAGGGC 
ACCCTGACTG TCTCTGAGCT GCAGAAAGTT GAGGAGCTGG TGCTCAATAT CATTCGTCAA 
AATGCAGATG TGCACGTAGA GGAGGTTCCT CTGTCGAGTG CTAAACAAAT TGCTGGCCTG 
AGGACTGTAG ATGAGCTCTA TCCAGACCCA GTGCGAGTGG TGTCCGTGGC TGTGCCTGTT 
TCGGATCTGC TTGCTTCAGA AAGAGCTGAG CAAACTTCAG TTGAGCTCTG CTGTGGCACG 
CATTTACTGA GGACGGGAGC AATTAGGGAC TTTGTTGTTG TATCTGAGCG ACAGATGATG 
AAAGGCGTTT GCCGTATTGT TGCCTTTACT GGAGATGACG CCATAAAGGC TAGAGAGGCA 
GGCCAAGCCT TGCAGGAGGA GTTGGAGTCT CTGGAGGCCC GGATGGGATT GTCAAACACA 
TTGTCTATTC AAGAAGCAAA GCGGCTTTCC AAAGAGGTTG GCCACCTAAT CAATAAAACA 
AAGAAGATAT TTCGAAGAAA GCTTGAAAAC GCTGACGTCC ATGGCAGTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009294684.1
CDS	10..2580
Translation	MAARITVVKHVHCALLITKLSCLSSGIRRCTALSFSVYRREFTSKRVRRKFIDFFREGYGHRVVPSSSVRPRGDPSLLFVNAGMNQFKPILLGCADPRSEMVSYRRVVNSQKCVRAGGKHNDLQDVGKDVYHHTFFEMLGNWSFGDYFKVEACAMAWRLLTEEYGIPAQRLYVSYFSGDPANGLPADEETRDIWLSMGLRADHVLPFGMKDNFWEMGETGPCGPCTEIHYDHIGNRNAALLVNADSPDVVEIWNLVFMQYNRESNGSLRALPQCSVDTGMGLERLVTVLQGKRSNYDTDLFTPLLSAIHQCSKAPAYQGRTGAADVAQLDMAYRVVADHIRTICVCIADGVYPGMAGAELVLRRILRRAVRFSTEVLQAPEGALASLVPTVAHLLGDAYPELNTESDRIMDLINQNEAHFLSSLKQGRRVIDRTLSNMDENSSFFPASVAWSLYRNLGFPLDLIDLMLEEKGKIVDKKEMKVLEDEYEKLGLQSEEDEGEKLDQLDLHSLAELQRQRVPHTDDSPKYCYSVGSDGQYVFEPCKASVLALYCDGALVSEVRQGQHCAVILDQTSFYAEQGGQANDQGYFTKDGLQDVLFPVKCVRLAGGYVVHKVTAAETLRTGDQVHLHVDEAWRLGCMINHTATHVLNFALRELLGPIVSQRGSHCSANRLRFDFSVKGTLTVSELQKVEELVLNIIRQNADVHVEEVPLSSAKQIAGLRTVDELYPDPVRVVSVAVPVSDLLASERAEQTSVELCCGTHLLRTGAIRDFVVVSERQMMKGVCRIVAFTGDDAIKAREAGQALQEELESLEARMGLSNTLSIQEAKRLSKEVGHLINKTKKIFRRKLENADVHGS

Target ORF information:

RefSeq Version	XM_009296409.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio alanyl-tRNA synthetase 2, mitochondrial (putative) (aars2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009296409.3

ORF Insert Sequence:

ATGGCCGCGC GCATTACAGT CGTGAAACAT GTTCACTGTG CACTTCTAAT TACAAAATTG 
AGCTGTTTGT CTTCGGGAAT AAGAAGATGC ACGGCTCTGT CATTCTCAGT ATATCGTAGG 
GAATTCACAT CTAAAAGAGT ACGGCGGAAG TTTATCGACT TTTTCAGGGA AGGTTATGGA 
CATCGGGTGG TTCCGTCATC CTCCGTACGC CCGAGAGGAG ACCCCAGTCT GCTATTCGTC 
AATGCAGGAA TGAACCAGTT TAAGCCGATT CTGCTGGGCT GTGCTGACCC TCGCAGTGAG 
ATGGTCTCGT ACCGGCGGGT GGTAAACAGT CAGAAGTGTG TCCGTGCTGG AGGCAAACAC 
AATGACCTAC AGGATGTGGG GAAGGACGTT TACCATCATA CCTTCTTCGA GATGCTGGGG 
AACTGGTCAT TCGGGGACTA CTTCAAGGTG GAAGCTTGTG CAATGGCCTG GCGTCTTTTA 
ACGGAAGAGT ATGGCATTCC TGCGCAGCGC CTGTATGTCT CGTACTTTTC TGGAGATCCA 
GCTAATGGTC TACCTGCTGA TGAGGAGACA CGGGACATCT GGCTCAGCAT GGGACTGAGA 
GCGGATCATG TGTTGCCATT TGGGATGAAG GATAACTTCT GGGAAATGGG AGAGACGGGC 
CCATGTGGCC CCTGCACGGA GATTCACTAC GATCACATTG GCAACAGGAA CGCTGCCTTA 
CTGGTCAATG CAGACAGTCC AGATGTAGTG GAAATCTGGA ACCTGGTATT CATGCAATAT 
AACAGGGAGA GCAATGGTAG TCTGCGAGCT CTTCCCCAGT GCAGTGTGGA TACTGGCATG 
GGTTTAGAGA GGCTGGTGAC GGTGCTGCAA GGCAAACGCT CCAACTACGA CACAGATCTC 
TTCACACCTC TATTATCTGC CATCCACCAG TGCTCTAAGG CTCCCGCATA TCAGGGCAGA 
ACCGGAGCGG CTGATGTGGC TCAGCTGGAC ATGGCCTATC GGGTCGTGGC GGATCACATC 
CGAACAATCT GTGTGTGTAT CGCTGACGGA GTTTACCCAG GCATGGCAGG CGCTGAGCTG 
GTGTTAAGAC GCATCTTAAG ACGTGCGGTT AGGTTTTCTA CAGAGGTGCT GCAGGCTCCT 
GAGGGTGCGC TGGCAAGTCT GGTGCCCACT GTAGCTCATC TACTGGGTGA TGCCTATCCT 
GAGCTGAACA CAGAGTCTGA TAGAATCATG GATCTCATTA ATCAGAATGA AGCCCACTTC 
CTTTCCTCGC TGAAACAGGG TAGGAGGGTG ATTGACAGGA CTCTCAGCAA CATGGACGAA 
AACTCTTCTT TTTTTCCAGC CTCGGTTGCC TGGTCCTTAT ATCGCAACCT GGGCTTTCCT 
CTGGATCTCA TTGACCTAAT GCTAGAGGAA AAAGGCAAGA TTGTTGATAA AAAGGAAATG 
AAGGTGCTGG AAGATGAATA TGAGAAGTTG GGTCTTCAGT CAGAGGAGGA TGAAGGTGAA 
AAGCTGGACC AGCTGGACCT TCATAGCCTG GCGGAGCTAC AACGGCAAAG AGTTCCTCAC 
ACAGACGACT CTCCAAAATA CTGTTACAGT GTGGGGTCCG ATGGACAATA CGTGTTTGAG 
CCCTGCAAAG CGTCTGTCCT GGCACTTTAC TGTGATGGGG CACTGGTTTC AGAGGTGCGT 
CAGGGTCAGC ACTGCGCTGT GATCCTGGAC CAGACCAGCT TTTATGCAGA GCAGGGCGGA 
CAGGCCAATG ACCAGGGTTA CTTCACCAAG GATGGGCTGC AGGATGTGTT GTTCCCTGTA 
AAGTGTGTCC GTCTCGCTGG GGGTTATGTG GTACACAAAG TCACAGCAGC AGAGACTTTG 
AGAACTGGAG ATCAAGTGCA TCTTCACGTG GATGAGGCTT GGCGGCTGGG CTGCATGATA 
AATCACACCG CCACCCATGT GCTGAACTTT GCTCTGCGAG AGTTGCTAGG TCCCATTGTC 
AGCCAACGAG GGTCTCATTG CAGTGCGAAT CGTCTACGCT TCGACTTCAG TGTCAAGGGC 
ACCCTGACTG TCTCTGAGCT GCAGAAAGTT GAGGAGCTGG TGCTCAATAT CATTCGTCAA 
AATGCAGATG TGCACGTAGA GGAGGTTCCT CTGTCGAGTG CTAAACAAAT TGCTGGCCTG 
AGGACTGTAG ATGAGCTCTA TCCAGACCCA GTGCGAGTGG TGTCCGTGGC TGTGCCTGTT 
TCGGATCTGC TTGCTTCAGA AAGAGCTGAG CAAACTTCAG TTGAGCTCTG CTGTGGCACG 
CATTTACTGA GGACGGGAGC AATTAGGGAC TTTGTTGTTG TATCTGAGCG ACAGATGATG 
AAAGGCGTTT GCCGTATTGT TGCCTTTACT GGAGATGACG CCATAAAGGC TAGAGAGGCA 
GGCCAAGCCT TGCAGGAGGA GTTGGAGTCT CTGGAGGCCC GGATGGGATT GTCAAACACA 
TTGTCTATTC AAGAAGCAAA GCGGCTTTCC AAAGAGGTTG GCCACCTAAT CAATAAAACA 
AAGAAGATAT TTCGAAGAAA GCTTGAAAAC GCTGACGTCC ATGGCAGTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

aars2 ( XM_021473512.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021329187.1 Danio rerio alanyl-tRNA synthetase 2, mitochondrial (putative) (aars2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa146178
Clone ID Related Accession (Same CDS sequence)	XM_021473512.1
Accession Version	XM_021473512.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3015bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	alanine--tRNA ligase, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395110.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGCGC GCATCACAGT CGTGAAATAT GTTCACTGTG CACTTCTAAC TACAAAATTG 
AGCTGTTTGT CTTCGGGAAT AAGAAGATGC TCTGCTCTGT CATGCTCAGT ATCTCGTAGG 
GAATTCACAT CTAAAAGAGT ACGGCGGAAG TTTATCGACT TTTTCAGGGA AGGTTATGGA 
CATCGGGTGG TTCCGTCTTC CTCCGTACGC CCGAGAGGAG ACCCCAGTCT GCTATTCGTC 
AATGCAGGAA TGAACCAGTT TAAGCCGATT CTGCTGGGCT GTGCTGACCC TCGCAGTGAG 
ATGGTCTCGT ACCGGCGGGT GGTAAACAGT CAGAAGTGTG TCCGTGCTGG AGGCAAACAC 
AATGACCTAC AGGATGTGGG GAAGGACGTT TACCATCATA CCTTCTTCGA GATGCTGGGG 
AACTGGTCAT TCGGGGACTA CTTCAAGGTG GAAGCTTGTG CAATGGCCTG GCGTCTTTTA 
ACGGAAGAGT ATGGCATTCC TGCGCAGCGC CTGTATGTCT CGTACTTTTC TGGAGATCCA 
GCTAATGGTC TACCTGCTGA TGAGGAGACA CGGGACATCT GGCTTAGCAT GGGACTGAGA 
GCGGATCATG TGTTGCCATT TGGGATGAAG GATAACTTCT GGGAAATGGG AGAGACGGGC 
CCATGTGGCC CCTGCACAGA GATTCACTAC GATCACATTG GCAACAGGAA CGCTGCCTCA 
CTGGTCAATG CAGACAGTCC AGATGTAGTG GAAATCTGGA ACCTGGTATT CATGCAATAT 
AACAGGGAGA GCAATGGTAG TCTGCGAGCT CTTCCCCAGT GCAGTGTGGA TACTGGCATG 
GGTTTAGAGA GGCTGGTGAC GGTGCTGCAA GGCAAACGCT CCAACTACGA CACAGATCTC 
TTCACACCTC TATTATCTGC CATCCACCAG TGCTCTAAGG CTCCCGCATA TCAGGGCAGA 
ACCGGAGCGG CTGATGTGGC TCAGCTGGAC ATGGCCTATC GGGTCGTGGC GGATCACATC 
CGAACAATCT GTGTGTGTAT CGCTGACGGA GTTTACCCAG GCATGGCAGG CGCTGAGCTG 
GTGTTAAGAC GCATCTTAAG ACGTGCGGTT AGGTTTTCTA CAGAGGTGCT GCAGGCTCCT 
GAGGGTGCAC TGGCAAGTCT GGTGCCCACT GTAGCTAATC TACTGGGTGA TGCCTATCCT 
GAGCTGAACA CAGAGTCTGA TAGAATCATG GATCTCATTA ATCAGAATGA AGCCCACTTC 
CTGTCCTCGC TGAAACAGGG TAGGAGGGTG ATTGACAGGA CTCTCAGCAA CATGGACGAA 
AACTCTTCTT TTTTTCCAGC CTCTGTTGCC TGGTCCTTAT ATCGTAACCT GGGCTTTCCT 
CTGGATCTCA TTGACCTTAT GCTAGAGGAA AAAGGCAAGA TTGTTGATAA AAAGGAAATG 
AAGGTGCTGG AAGATGAATA TGAGAAGTTG GGTCTTCAGT CAGAGGAGGA TGAAGGTGAA 
AAGCTGGACC AGCTGGACCT TCATAGCCTG GCAGAGCTAC AACGGCAAAG AGTTCCTCAC 
ACAGACGACT CTCCAAAATA CTGTTACAGT GTGGGGTCCG ATGGACAATA CGTGTTTGAG 
CCCTGCAAAG CGTCTGTCCT GGCACTTTAC TGTGATGGGG CACTGGTTTC AGAGGTGCGT 
CAGGGTCAGC ACTGCGCTGT GATCCTGGAC CAGACCAGCT TTTATGCAGA GCAGGGCGGA 
CAGGCCAATG ACCAGGGTTA CTTCACCAAG GATGGGCTGC AGGATGTGTT GTTCCCTGTA 
AAGTGTGTCC GTCTCGCTGG GGGTTATGTG GTACACAAAG TCACAGCAGC AGAGACTTTG 
AGAACTGGAG ATCAAGTGCA TCTTCACGTG GATGAGGCTT GGCGGCTGGG CTGCATGATA 
AATCACACCG CCACCCATGT GCTGAACTTT GCTCTGCGAG AGTTGCTAGG TCCCGTTGTC 
AGCCAACGAG GGTCTCATTG CAGTGCGAAT CGTCTACGCT TCGACTTCAG TGTCAAGGGC 
TCCCTGACAG TCTCTGAGCT GCAGAAAGTT GAGGAGCTGG TGCTCAATAT CATTCGTCAA 
AATGCAGATG TGCACGTAGA GGAGGTTCCT CTGTCGAGTG CTAAACAAAT TGCTGGCCTG 
AGGACTGTAG ATGAGCTCTA TCCAGACCCA GTGCGAGTGG TGTCTGTGGC TGTGCCTGTT 
TCGGATCTGC TTGCTTCAGA AAGAGCTGAG CAAACTTCAG TTGAGCTCTG CTGTGGCACG 
CATTTACTGA GGACGGGAGC AATTAGGGAC TTTGTTGTTG TATCTGAGCG ACAGATGATG 
AAAGGCGTTT GCCGTATTGT TGCCTTTACT GGAGATGACG CCATAAAGGC TAGAGAGGCA 
GGCCAAGCCT TGCAGGAGGA GTTGGAGTCT CTGGAGGCCC GGATGGGATT GTCTAACACA 
TTGTCTATTC ATGAAGCAAA GCGGCTTTCC AAAGAGGTTG GCCACCAAAT CAATGCAGTT 
GATATCACAC CTATGCCACA ATGGAAGAGA CTAGAGTTGC AGACTCGTCT GAAAGCGGTG 
CTGAAGAGCA GCAATAACTC CATTCGAAAG TTGGAGATCA AAGAGGCTGC CTTAAAAGCC 
AAGGAGCTTC TGGACAAGCA CTCCAATAAA CCTGTCGTGG TGGATTTGAT CCAGACTGAC 
TCTATTTCAG TGCTAATGAA GACTGTGGTC CAGCTGAGCA AGAGCACTCC AAAGTCCTTG 
GTCATGTTGT TCTCTCACTG GGAGTCATCA GGGAAGCTGC TCTGTGCCTG TCTAGTACCC 
AAAGGCTCTA CTGGTGGCTC GGCGATCGAA TGGGCTCTTT CTGTGTGTTC ACGCTTGGGT 
GGAAATGCAG AAGGTGCAAA GGACACCGCT AAAGGTGTGG GGAATGCAGT CAAAGTTGAG 
GATGTTGCAG AGATCCTGCA CTGGGCTGAA GAATTTGCAC ACAACAAAAT CAGAAACGTG 
CCCCGAACTG ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021329187.1
CDS	13..3027
Translation	MAARITVVKYVHCALLTTKLSCLSSGIRRCSALSCSVSRREFTSKRVRRKFIDFFREGYGHRVVPSSSVRPRGDPSLLFVNAGMNQFKPILLGCADPRSEMVSYRRVVNSQKCVRAGGKHNDLQDVGKDVYHHTFFEMLGNWSFGDYFKVEACAMAWRLLTEEYGIPAQRLYVSYFSGDPANGLPADEETRDIWLSMGLRADHVLPFGMKDNFWEMGETGPCGPCTEIHYDHIGNRNAASLVNADSPDVVEIWNLVFMQYNRESNGSLRALPQCSVDTGMGLERLVTVLQGKRSNYDTDLFTPLLSAIHQCSKAPAYQGRTGAADVAQLDMAYRVVADHIRTICVCIADGVYPGMAGAELVLRRILRRAVRFSTEVLQAPEGALASLVPTVANLLGDAYPELNTESDRIMDLINQNEAHFLSSLKQGRRVIDRTLSNMDENSSFFPASVAWSLYRNLGFPLDLIDLMLEEKGKIVDKKEMKVLEDEYEKLGLQSEEDEGEKLDQLDLHSLAELQRQRVPHTDDSPKYCYSVGSDGQYVFEPCKASVLALYCDGALVSEVRQGQHCAVILDQTSFYAEQGGQANDQGYFTKDGLQDVLFPVKCVRLAGGYVVHKVTAAETLRTGDQVHLHVDEAWRLGCMINHTATHVLNFALRELLGPVVSQRGSHCSANRLRFDFSVKGSLTVSELQKVEELVLNIIRQNADVHVEEVPLSSAKQIAGLRTVDELYPDPVRVVSVAVPVSDLLASERAEQTSVELCCGTHLLRTGAIRDFVVVSERQMMKGVCRIVAFTGDDAIKAREAGQALQEELESLEARMGLSNTLSIHEAKRLSKEVGHQINAVDITPMPQWKRLELQTRLKAVLKSSNNSIRKLEIKEAALKAKELLDKHSNKPVVVDLIQTDSISVLMKTVVQLSKSTPKSLVMLFSHWESSGKLLCACLVPKGSTGGSAIEWALSVCSRLGGNAEGAKDTAKGVGNAVKVEDVAEILHWAEEFAHNKIRNVPRTD

Target ORF information:

RefSeq Version	XM_021473512.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio alanyl-tRNA synthetase 2, mitochondrial (putative) (aars2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021473512.1

ORF Insert Sequence:

ATGGCCGCGC GCATCACAGT CGTGAAATAT GTTCACTGTG CACTTCTAAC TACAAAATTG 
AGCTGTTTGT CTTCGGGAAT AAGAAGATGC TCTGCTCTGT CATGCTCAGT ATCTCGTAGG 
GAATTCACAT CTAAAAGAGT ACGGCGGAAG TTTATCGACT TTTTCAGGGA AGGTTATGGA 
CATCGGGTGG TTCCGTCTTC CTCCGTACGC CCGAGAGGAG ACCCCAGTCT GCTATTCGTC 
AATGCAGGAA TGAACCAGTT TAAGCCGATT CTGCTGGGCT GTGCTGACCC TCGCAGTGAG 
ATGGTCTCGT ACCGGCGGGT GGTAAACAGT CAGAAGTGTG TCCGTGCTGG AGGCAAACAC 
AATGACCTAC AGGATGTGGG GAAGGACGTT TACCATCATA CCTTCTTCGA GATGCTGGGG 
AACTGGTCAT TCGGGGACTA CTTCAAGGTG GAAGCTTGTG CAATGGCCTG GCGTCTTTTA 
ACGGAAGAGT ATGGCATTCC TGCGCAGCGC CTGTATGTCT CGTACTTTTC TGGAGATCCA 
GCTAATGGTC TACCTGCTGA TGAGGAGACA CGGGACATCT GGCTTAGCAT GGGACTGAGA 
GCGGATCATG TGTTGCCATT TGGGATGAAG GATAACTTCT GGGAAATGGG AGAGACGGGC 
CCATGTGGCC CCTGCACAGA GATTCACTAC GATCACATTG GCAACAGGAA CGCTGCCTCA 
CTGGTCAATG CAGACAGTCC AGATGTAGTG GAAATCTGGA ACCTGGTATT CATGCAATAT 
AACAGGGAGA GCAATGGTAG TCTGCGAGCT CTTCCCCAGT GCAGTGTGGA TACTGGCATG 
GGTTTAGAGA GGCTGGTGAC GGTGCTGCAA GGCAAACGCT CCAACTACGA CACAGATCTC 
TTCACACCTC TATTATCTGC CATCCACCAG TGCTCTAAGG CTCCCGCATA TCAGGGCAGA 
ACCGGAGCGG CTGATGTGGC TCAGCTGGAC ATGGCCTATC GGGTCGTGGC GGATCACATC 
CGAACAATCT GTGTGTGTAT CGCTGACGGA GTTTACCCAG GCATGGCAGG CGCTGAGCTG 
GTGTTAAGAC GCATCTTAAG ACGTGCGGTT AGGTTTTCTA CAGAGGTGCT GCAGGCTCCT 
GAGGGTGCAC TGGCAAGTCT GGTGCCCACT GTAGCTAATC TACTGGGTGA TGCCTATCCT 
GAGCTGAACA CAGAGTCTGA TAGAATCATG GATCTCATTA ATCAGAATGA AGCCCACTTC 
CTGTCCTCGC TGAAACAGGG TAGGAGGGTG ATTGACAGGA CTCTCAGCAA CATGGACGAA 
AACTCTTCTT TTTTTCCAGC CTCTGTTGCC TGGTCCTTAT ATCGTAACCT GGGCTTTCCT 
CTGGATCTCA TTGACCTTAT GCTAGAGGAA AAAGGCAAGA TTGTTGATAA AAAGGAAATG 
AAGGTGCTGG AAGATGAATA TGAGAAGTTG GGTCTTCAGT CAGAGGAGGA TGAAGGTGAA 
AAGCTGGACC AGCTGGACCT TCATAGCCTG GCAGAGCTAC AACGGCAAAG AGTTCCTCAC 
ACAGACGACT CTCCAAAATA CTGTTACAGT GTGGGGTCCG ATGGACAATA CGTGTTTGAG 
CCCTGCAAAG CGTCTGTCCT GGCACTTTAC TGTGATGGGG CACTGGTTTC AGAGGTGCGT 
CAGGGTCAGC ACTGCGCTGT GATCCTGGAC CAGACCAGCT TTTATGCAGA GCAGGGCGGA 
CAGGCCAATG ACCAGGGTTA CTTCACCAAG GATGGGCTGC AGGATGTGTT GTTCCCTGTA 
AAGTGTGTCC GTCTCGCTGG GGGTTATGTG GTACACAAAG TCACAGCAGC AGAGACTTTG 
AGAACTGGAG ATCAAGTGCA TCTTCACGTG GATGAGGCTT GGCGGCTGGG CTGCATGATA 
AATCACACCG CCACCCATGT GCTGAACTTT GCTCTGCGAG AGTTGCTAGG TCCCGTTGTC 
AGCCAACGAG GGTCTCATTG CAGTGCGAAT CGTCTACGCT TCGACTTCAG TGTCAAGGGC 
TCCCTGACAG TCTCTGAGCT GCAGAAAGTT GAGGAGCTGG TGCTCAATAT CATTCGTCAA 
AATGCAGATG TGCACGTAGA GGAGGTTCCT CTGTCGAGTG CTAAACAAAT TGCTGGCCTG 
AGGACTGTAG ATGAGCTCTA TCCAGACCCA GTGCGAGTGG TGTCTGTGGC TGTGCCTGTT 
TCGGATCTGC TTGCTTCAGA AAGAGCTGAG CAAACTTCAG TTGAGCTCTG CTGTGGCACG 
CATTTACTGA GGACGGGAGC AATTAGGGAC TTTGTTGTTG TATCTGAGCG ACAGATGATG 
AAAGGCGTTT GCCGTATTGT TGCCTTTACT GGAGATGACG CCATAAAGGC TAGAGAGGCA 
GGCCAAGCCT TGCAGGAGGA GTTGGAGTCT CTGGAGGCCC GGATGGGATT GTCTAACACA 
TTGTCTATTC ATGAAGCAAA GCGGCTTTCC AAAGAGGTTG GCCACCAAAT CAATGCAGTT 
GATATCACAC CTATGCCACA ATGGAAGAGA CTAGAGTTGC AGACTCGTCT GAAAGCGGTG 
CTGAAGAGCA GCAATAACTC CATTCGAAAG TTGGAGATCA AAGAGGCTGC CTTAAAAGCC 
AAGGAGCTTC TGGACAAGCA CTCCAATAAA CCTGTCGTGG TGGATTTGAT CCAGACTGAC 
TCTATTTCAG TGCTAATGAA GACTGTGGTC CAGCTGAGCA AGAGCACTCC AAAGTCCTTG 
GTCATGTTGT TCTCTCACTG GGAGTCATCA GGGAAGCTGC TCTGTGCCTG TCTAGTACCC 
AAAGGCTCTA CTGGTGGCTC GGCGATCGAA TGGGCTCTTT CTGTGTGTTC ACGCTTGGGT 
GGAAATGCAG AAGGTGCAAA GGACACCGCT AAAGGTGTGG GGAATGCAGT CAAAGTTGAG 
GATGTTGCAG AGATCCTGCA CTGGGCTGAA GAATTTGCAC ACAACAAAAT CAGAAACGTG 
CCCCGAACTG ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

aars2 ( XM_001332352.8 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_001332388.5 Danio rerio alanyl-tRNA synthetase 2, mitochondrial (putative) (aars2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa27040
Clone ID Related Accession (Same CDS sequence)	XM_001332352.8 , XM_001332352.7
Accession Version	XM_001332352.8 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3015bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	alanine--tRNA ligase, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007133.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_001332352.7. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGCGC GCATTACAGT CGTGAAACAT GTTCACTGTG CACTTCTAAT TACAAAATTG 
AGCTGTTTGT CTTCGGGAAT AAGAAGATGC ACGGCTCTGT CATTCTCAGT ATATCGTAGG 
GAATTCACAT CTAAAAGAGT ACGGCGGAAG TTTATCGACT TTTTCAGGGA AGGTTATGGA 
CATCGGGTGG TTCCGTCATC CTCCGTACGC CCGAGAGGAG ACCCCAGTCT GCTATTCGTC 
AATGCAGGAA TGAACCAGTT TAAGCCGATT CTGCTGGGCT GTGCTGACCC TCGCAGTGAG 
ATGGTCTCGT ACCGGCGGGT GGTAAACAGT CAGAAGTGTG TCCGTGCTGG AGGCAAACAC 
AATGACCTAC AGGATGTGGG GAAGGACGTT TACCATCATA CCTTCTTCGA GATGCTGGGG 
AACTGGTCAT TCGGGGACTA CTTCAAGGTG GAAGCTTGTG CAATGGCCTG GCGTCTTTTA 
ACGGAAGAGT ATGGCATTCC TGCGCAGCGC CTGTATGTCT CGTACTTTTC TGGAGATCCA 
GCTAATGGTC TACCTGCTGA TGAGGAGACA CGGGACATCT GGCTCAGCAT GGGACTGAGA 
GCGGATCATG TGTTGCCATT TGGGATGAAG GATAACTTCT GGGAAATGGG AGAGACGGGC 
CCATGTGGCC CCTGCACGGA GATTCACTAC GATCACATTG GCAACAGGAA CGCTGCCTTA 
CTGGTCAATG CAGACAGTCC AGATGTAGTG GAAATCTGGA ACCTGGTATT CATGCAATAT 
AACAGGGAGA GCAATGGTAG TCTGCGAGCT CTTCCCCAGT GCAGTGTGGA TACTGGCATG 
GGTTTAGAGA GGCTGGTGAC GGTGCTGCAA GGCAAACGCT CCAACTACGA CACAGATCTC 
TTCACACCTC TATTATCTGC CATCCACCAG TGCTCTAAGG CTCCCGCATA TCAGGGCAGA 
ACCGGAGCGG CTGATGTGGC TCAGCTGGAC ATGGCCTATC GGGTCGTGGC GGATCACATC 
CGAACAATCT GTGTGTGTAT CGCTGACGGA GTTTACCCAG GCATGGCAGG CGCTGAGCTG 
GTGTTAAGAC GCATCTTAAG ACGTGCGGTT AGGTTTTCTA CAGAGGTGCT GCAGGCTCCT 
GAGGGTGCGC TGGCAAGTCT GGTGCCCACT GTAGCTCATC TACTGGGTGA TGCCTATCCT 
GAGCTGAACA CAGAGTCTGA TAGAATCATG GATCTCATTA ATCAGAATGA AGCCCACTTC 
CTTTCCTCGC TGAAACAGGG TAGGAGGGTG ATTGACAGGA CTCTCAGCAA CATGGACGAA 
AACTCTTCTT TTTTTCCAGC CTCGGTTGCC TGGTCCTTAT ATCGCAACCT GGGCTTTCCT 
CTGGATCTCA TTGACCTAAT GCTAGAGGAA AAAGGCAAGA TTGTTGATAA AAAGGAAATG 
AAGGTGCTGG AAGATGAATA TGAGAAGTTG GGTCTTCAGT CAGAGGAGGA TGAAGGTGAA 
AAGCTGGACC AGCTGGACCT TCATAGCCTG GCGGAGCTAC AACGGCAAAG AGTTCCTCAC 
ACAGACGACT CTCCAAAATA CTGTTACAGT GTGGGGTCCG ATGGACAATA CGTGTTTGAG 
CCCTGCAAAG CGTCTGTCCT GGCACTTTAC TGTGATGGGG CACTGGTTTC AGAGGTGCGT 
CAGGGTCAGC ACTGCGCTGT GATCCTGGAC CAGACCAGCT TTTATGCAGA GCAGGGCGGA 
CAGGCCAATG ACCAGGGTTA CTTCACCAAG GATGGGCTGC AGGATGTGTT GTTCCCTGTA 
AAGTGTGTCC GTCTCGCTGG GGGTTATGTG GTACACAAAG TCACAGCAGC AGAGACTTTG 
AGAACTGGAG ATCAAGTGCA TCTTCACGTG GATGAGGCTT GGCGGCTGGG CTGCATGATA 
AATCACACCG CCACCCATGT GCTGAACTTT GCTCTGCGAG AGTTGCTAGG TCCCATTGTC 
AGCCAACGAG GGTCTCATTG CAGTGCGAAT CGTCTACGCT TCGACTTCAG TGTCAAGGGC 
ACCCTGACTG TCTCTGAGCT GCAGAAAGTT GAGGAGCTGG TGCTCAATAT CATTCGTCAA 
AATGCAGATG TGCACGTAGA GGAGGTTCCT CTGTCGAGTG CTAAACAAAT TGCTGGCCTG 
AGGACTGTAG ATGAGCTCTA TCCAGACCCA GTGCGAGTGG TGTCCGTGGC TGTGCCTGTT 
TCGGATCTGC TTGCTTCAGA AAGAGCTGAG CAAACTTCAG TTGAGCTCTG CTGTGGCACG 
CATTTACTGA GGACGGGAGC AATTAGGGAC TTTGTTGTTG TATCTGAGCG ACAGATGATG 
AAAGGCGTTT GCCGTATTGT TGCCTTTACT GGAGATGACG CCATAAAGGC TAGAGAGGCA 
GGCCAAGCCT TGCAGGAGGA GTTGGAGTCT CTGGAGGCCC GGATGGGATT GTCAAACACA 
TTGTCTATTC AAGAAGCAAA GCGGCTTTCC AAAGAGGTTG GCCACCTAAT CAATGCAGTT 
GATATCACAC CTATGCCACA ATGGAAGAGA CTAGAGTTGC AGACTCGTCT GAAAGCGATG 
CTGAAGAGCA GCAATAACTC TATTCGAAAG TTGGAGATCA AAGAGGCTGC CTTAAAAGCC 
AAGGAGCTGC TGGACAAACA CTCCAATAAA CCTGTCGTGG TGGATTTGAT CCAGACTGAC 
TCTATTTCAG TTCTAATGAA GACTGTGGTC CAGCTGAGCA AGAGCACTCC AAAGTCCTTG 
GTCATGTTGT TCTCTCACTG GGAGTCATCA GGGAAGCTGC TCTGTGCCTG TCTAGTACCC 
AAAGGCTCTA CTGGTGGCTC GGCGATCGAA TGGGCTCTTT CTGTGTGTTC ACGCTTGGGT 
GGAAATGCAG AAGGTGCAAA GGACACCGCT AAAGGTGTGG GGAATGCAGT CAAAGTCGAG 
GATGTTGCAG AGATCCTGCA CTGGGCTGAA GAATTTGCAC ACAACAAAAT CAGAAACGTG 
CCCCGAACTG ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001332388.5
CDS	10..3024
Translation	MAARITVVKHVHCALLITKLSCLSSGIRRCTALSFSVYRREFTSKRVRRKFIDFFREGYGHRVVPSSSVRPRGDPSLLFVNAGMNQFKPILLGCADPRSEMVSYRRVVNSQKCVRAGGKHNDLQDVGKDVYHHTFFEMLGNWSFGDYFKVEACAMAWRLLTEEYGIPAQRLYVSYFSGDPANGLPADEETRDIWLSMGLRADHVLPFGMKDNFWEMGETGPCGPCTEIHYDHIGNRNAALLVNADSPDVVEIWNLVFMQYNRESNGSLRALPQCSVDTGMGLERLVTVLQGKRSNYDTDLFTPLLSAIHQCSKAPAYQGRTGAADVAQLDMAYRVVADHIRTICVCIADGVYPGMAGAELVLRRILRRAVRFSTEVLQAPEGALASLVPTVAHLLGDAYPELNTESDRIMDLINQNEAHFLSSLKQGRRVIDRTLSNMDENSSFFPASVAWSLYRNLGFPLDLIDLMLEEKGKIVDKKEMKVLEDEYEKLGLQSEEDEGEKLDQLDLHSLAELQRQRVPHTDDSPKYCYSVGSDGQYVFEPCKASVLALYCDGALVSEVRQGQHCAVILDQTSFYAEQGGQANDQGYFTKDGLQDVLFPVKCVRLAGGYVVHKVTAAETLRTGDQVHLHVDEAWRLGCMINHTATHVLNFALRELLGPIVSQRGSHCSANRLRFDFSVKGTLTVSELQKVEELVLNIIRQNADVHVEEVPLSSAKQIAGLRTVDELYPDPVRVVSVAVPVSDLLASERAEQTSVELCCGTHLLRTGAIRDFVVVSERQMMKGVCRIVAFTGDDAIKAREAGQALQEELESLEARMGLSNTLSIQEAKRLSKEVGHLINAVDITPMPQWKRLELQTRLKAMLKSSNNSIRKLEIKEAALKAKELLDKHSNKPVVVDLIQTDSISVLMKTVVQLSKSTPKSLVMLFSHWESSGKLLCACLVPKGSTGGSAIEWALSVCSRLGGNAEGAKDTAKGVGNAVKVEDVAEILHWAEEFAHNKIRNVPRTD

Target ORF information:

RefSeq Version	XM_001332352.8
Organism	Danio rerio(zebrafish)
Definition	Danio rerio alanyl-tRNA synthetase 2, mitochondrial (putative) (aars2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001332352.8

ORF Insert Sequence:

ATGGCCGCGC GCATTACAGT CGTGAAACAT GTTCACTGTG CACTTCTAAT TACAAAATTG 
AGCTGTTTGT CTTCGGGAAT AAGAAGATGC ACGGCTCTGT CATTCTCAGT ATATCGTAGG 
GAATTCACAT CTAAAAGAGT ACGGCGGAAG TTTATCGACT TTTTCAGGGA AGGTTATGGA 
CATCGGGTGG TTCCGTCATC CTCCGTACGC CCGAGAGGAG ACCCCAGTCT GCTATTCGTC 
AATGCAGGAA TGAACCAGTT TAAGCCGATT CTGCTGGGCT GTGCTGACCC TCGCAGTGAG 
ATGGTCTCGT ACCGGCGGGT GGTAAACAGT CAGAAGTGTG TCCGTGCTGG AGGCAAACAC 
AATGACCTAC AGGATGTGGG GAAGGACGTT TACCATCATA CCTTCTTCGA GATGCTGGGG 
AACTGGTCAT TCGGGGACTA CTTCAAGGTG GAAGCTTGTG CAATGGCCTG GCGTCTTTTA 
ACGGAAGAGT ATGGCATTCC TGCGCAGCGC CTGTATGTCT CGTACTTTTC TGGAGATCCA 
GCTAATGGTC TACCTGCTGA TGAGGAGACA CGGGACATCT GGCTCAGCAT GGGACTGAGA 
GCGGATCATG TGTTGCCATT TGGGATGAAG GATAACTTCT GGGAAATGGG AGAGACGGGC 
CCATGTGGCC CCTGCACGGA GATTCACTAC GATCACATTG GCAACAGGAA CGCTGCCTTA 
CTGGTCAATG CAGACAGTCC AGATGTAGTG GAAATCTGGA ACCTGGTATT CATGCAATAT 
AACAGGGAGA GCAATGGTAG TCTGCGAGCT CTTCCCCAGT GCAGTGTGGA TACTGGCATG 
GGTTTAGAGA GGCTGGTGAC GGTGCTGCAA GGCAAACGCT CCAACTACGA CACAGATCTC 
TTCACACCTC TATTATCTGC CATCCACCAG TGCTCTAAGG CTCCCGCATA TCAGGGCAGA 
ACCGGAGCGG CTGATGTGGC TCAGCTGGAC ATGGCCTATC GGGTCGTGGC GGATCACATC 
CGAACAATCT GTGTGTGTAT CGCTGACGGA GTTTACCCAG GCATGGCAGG CGCTGAGCTG 
GTGTTAAGAC GCATCTTAAG ACGTGCGGTT AGGTTTTCTA CAGAGGTGCT GCAGGCTCCT 
GAGGGTGCGC TGGCAAGTCT GGTGCCCACT GTAGCTCATC TACTGGGTGA TGCCTATCCT 
GAGCTGAACA CAGAGTCTGA TAGAATCATG GATCTCATTA ATCAGAATGA AGCCCACTTC 
CTTTCCTCGC TGAAACAGGG TAGGAGGGTG ATTGACAGGA CTCTCAGCAA CATGGACGAA 
AACTCTTCTT TTTTTCCAGC CTCGGTTGCC TGGTCCTTAT ATCGCAACCT GGGCTTTCCT 
CTGGATCTCA TTGACCTAAT GCTAGAGGAA AAAGGCAAGA TTGTTGATAA AAAGGAAATG 
AAGGTGCTGG AAGATGAATA TGAGAAGTTG GGTCTTCAGT CAGAGGAGGA TGAAGGTGAA 
AAGCTGGACC AGCTGGACCT TCATAGCCTG GCGGAGCTAC AACGGCAAAG AGTTCCTCAC 
ACAGACGACT CTCCAAAATA CTGTTACAGT GTGGGGTCCG ATGGACAATA CGTGTTTGAG 
CCCTGCAAAG CGTCTGTCCT GGCACTTTAC TGTGATGGGG CACTGGTTTC AGAGGTGCGT 
CAGGGTCAGC ACTGCGCTGT GATCCTGGAC CAGACCAGCT TTTATGCAGA GCAGGGCGGA 
CAGGCCAATG ACCAGGGTTA CTTCACCAAG GATGGGCTGC AGGATGTGTT GTTCCCTGTA 
AAGTGTGTCC GTCTCGCTGG GGGTTATGTG GTACACAAAG TCACAGCAGC AGAGACTTTG 
AGAACTGGAG ATCAAGTGCA TCTTCACGTG GATGAGGCTT GGCGGCTGGG CTGCATGATA 
AATCACACCG CCACCCATGT GCTGAACTTT GCTCTGCGAG AGTTGCTAGG TCCCATTGTC 
AGCCAACGAG GGTCTCATTG CAGTGCGAAT CGTCTACGCT TCGACTTCAG TGTCAAGGGC 
ACCCTGACTG TCTCTGAGCT GCAGAAAGTT GAGGAGCTGG TGCTCAATAT CATTCGTCAA 
AATGCAGATG TGCACGTAGA GGAGGTTCCT CTGTCGAGTG CTAAACAAAT TGCTGGCCTG 
AGGACTGTAG ATGAGCTCTA TCCAGACCCA GTGCGAGTGG TGTCCGTGGC TGTGCCTGTT 
TCGGATCTGC TTGCTTCAGA AAGAGCTGAG CAAACTTCAG TTGAGCTCTG CTGTGGCACG 
CATTTACTGA GGACGGGAGC AATTAGGGAC TTTGTTGTTG TATCTGAGCG ACAGATGATG 
AAAGGCGTTT GCCGTATTGT TGCCTTTACT GGAGATGACG CCATAAAGGC TAGAGAGGCA 
GGCCAAGCCT TGCAGGAGGA GTTGGAGTCT CTGGAGGCCC GGATGGGATT GTCAAACACA 
TTGTCTATTC AAGAAGCAAA GCGGCTTTCC AAAGAGGTTG GCCACCTAAT CAATGCAGTT 
GATATCACAC CTATGCCACA ATGGAAGAGA CTAGAGTTGC AGACTCGTCT GAAAGCGATG 
CTGAAGAGCA GCAATAACTC TATTCGAAAG TTGGAGATCA AAGAGGCTGC CTTAAAAGCC 
AAGGAGCTGC TGGACAAACA CTCCAATAAA CCTGTCGTGG TGGATTTGAT CCAGACTGAC 
TCTATTTCAG TTCTAATGAA GACTGTGGTC CAGCTGAGCA AGAGCACTCC AAAGTCCTTG 
GTCATGTTGT TCTCTCACTG GGAGTCATCA GGGAAGCTGC TCTGTGCCTG TCTAGTACCC 
AAAGGCTCTA CTGGTGGCTC GGCGATCGAA TGGGCTCTTT CTGTGTGTTC ACGCTTGGGT 
GGAAATGCAG AAGGTGCAAA GGACACCGCT AAAGGTGTGG GGAATGCAGT CAAAGTCGAG 
GATGTTGCAG AGATCCTGCA CTGGGCTGAA GAATTTGCAC ACAACAAAAT CAGAAACGTG 
CCCCGAACTG ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

aars2 ( XM_001332352.7 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_001332388.5 Danio rerio alanyl-tRNA synthetase 2, mitochondrial (putative) (aars2), transcript variant X1, mRNA.

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CloneID	ODa27040
Clone ID Related Accession (Same CDS sequence)	XM_001332352.8 , XM_001332352.7
Accession Version	XM_001332352.7	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3015bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	alanine--tRNA ligase, mitochondrial isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007133.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_001332352.6. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGCGC GCATTACAGT CGTGAAACAT GTTCACTGTG CACTTCTAAT TACAAAATTG 
AGCTGTTTGT CTTCGGGAAT AAGAAGATGC ACGGCTCTGT CATTCTCAGT ATATCGTAGG 
GAATTCACAT CTAAAAGAGT ACGGCGGAAG TTTATCGACT TTTTCAGGGA AGGTTATGGA 
CATCGGGTGG TTCCGTCATC CTCCGTACGC CCGAGAGGAG ACCCCAGTCT GCTATTCGTC 
AATGCAGGAA TGAACCAGTT TAAGCCGATT CTGCTGGGCT GTGCTGACCC TCGCAGTGAG 
ATGGTCTCGT ACCGGCGGGT GGTAAACAGT CAGAAGTGTG TCCGTGCTGG AGGCAAACAC 
AATGACCTAC AGGATGTGGG GAAGGACGTT TACCATCATA CCTTCTTCGA GATGCTGGGG 
AACTGGTCAT TCGGGGACTA CTTCAAGGTG GAAGCTTGTG CAATGGCCTG GCGTCTTTTA 
ACGGAAGAGT ATGGCATTCC TGCGCAGCGC CTGTATGTCT CGTACTTTTC TGGAGATCCA 
GCTAATGGTC TACCTGCTGA TGAGGAGACA CGGGACATCT GGCTCAGCAT GGGACTGAGA 
GCGGATCATG TGTTGCCATT TGGGATGAAG GATAACTTCT GGGAAATGGG AGAGACGGGC 
CCATGTGGCC CCTGCACGGA GATTCACTAC GATCACATTG GCAACAGGAA CGCTGCCTTA 
CTGGTCAATG CAGACAGTCC AGATGTAGTG GAAATCTGGA ACCTGGTATT CATGCAATAT 
AACAGGGAGA GCAATGGTAG TCTGCGAGCT CTTCCCCAGT GCAGTGTGGA TACTGGCATG 
GGTTTAGAGA GGCTGGTGAC GGTGCTGCAA GGCAAACGCT CCAACTACGA CACAGATCTC 
TTCACACCTC TATTATCTGC CATCCACCAG TGCTCTAAGG CTCCCGCATA TCAGGGCAGA 
ACCGGAGCGG CTGATGTGGC TCAGCTGGAC ATGGCCTATC GGGTCGTGGC GGATCACATC 
CGAACAATCT GTGTGTGTAT CGCTGACGGA GTTTACCCAG GCATGGCAGG CGCTGAGCTG 
GTGTTAAGAC GCATCTTAAG ACGTGCGGTT AGGTTTTCTA CAGAGGTGCT GCAGGCTCCT 
GAGGGTGCGC TGGCAAGTCT GGTGCCCACT GTAGCTCATC TACTGGGTGA TGCCTATCCT 
GAGCTGAACA CAGAGTCTGA TAGAATCATG GATCTCATTA ATCAGAATGA AGCCCACTTC 
CTTTCCTCGC TGAAACAGGG TAGGAGGGTG ATTGACAGGA CTCTCAGCAA CATGGACGAA 
AACTCTTCTT TTTTTCCAGC CTCGGTTGCC TGGTCCTTAT ATCGCAACCT GGGCTTTCCT 
CTGGATCTCA TTGACCTAAT GCTAGAGGAA AAAGGCAAGA TTGTTGATAA AAAGGAAATG 
AAGGTGCTGG AAGATGAATA TGAGAAGTTG GGTCTTCAGT CAGAGGAGGA TGAAGGTGAA 
AAGCTGGACC AGCTGGACCT TCATAGCCTG GCGGAGCTAC AACGGCAAAG AGTTCCTCAC 
ACAGACGACT CTCCAAAATA CTGTTACAGT GTGGGGTCCG ATGGACAATA CGTGTTTGAG 
CCCTGCAAAG CGTCTGTCCT GGCACTTTAC TGTGATGGGG CACTGGTTTC AGAGGTGCGT 
CAGGGTCAGC ACTGCGCTGT GATCCTGGAC CAGACCAGCT TTTATGCAGA GCAGGGCGGA 
CAGGCCAATG ACCAGGGTTA CTTCACCAAG GATGGGCTGC AGGATGTGTT GTTCCCTGTA 
AAGTGTGTCC GTCTCGCTGG GGGTTATGTG GTACACAAAG TCACAGCAGC AGAGACTTTG 
AGAACTGGAG ATCAAGTGCA TCTTCACGTG GATGAGGCTT GGCGGCTGGG CTGCATGATA 
AATCACACCG CCACCCATGT GCTGAACTTT GCTCTGCGAG AGTTGCTAGG TCCCATTGTC 
AGCCAACGAG GGTCTCATTG CAGTGCGAAT CGTCTACGCT TCGACTTCAG TGTCAAGGGC 
ACCCTGACTG TCTCTGAGCT GCAGAAAGTT GAGGAGCTGG TGCTCAATAT CATTCGTCAA 
AATGCAGATG TGCACGTAGA GGAGGTTCCT CTGTCGAGTG CTAAACAAAT TGCTGGCCTG 
AGGACTGTAG ATGAGCTCTA TCCAGACCCA GTGCGAGTGG TGTCCGTGGC TGTGCCTGTT 
TCGGATCTGC TTGCTTCAGA AAGAGCTGAG CAAACTTCAG TTGAGCTCTG CTGTGGCACG 
CATTTACTGA GGACGGGAGC AATTAGGGAC TTTGTTGTTG TATCTGAGCG ACAGATGATG 
AAAGGCGTTT GCCGTATTGT TGCCTTTACT GGAGATGACG CCATAAAGGC TAGAGAGGCA 
GGCCAAGCCT TGCAGGAGGA GTTGGAGTCT CTGGAGGCCC GGATGGGATT GTCAAACACA 
TTGTCTATTC AAGAAGCAAA GCGGCTTTCC AAAGAGGTTG GCCACCTAAT CAATGCAGTT 
GATATCACAC CTATGCCACA ATGGAAGAGA CTAGAGTTGC AGACTCGTCT GAAAGCGATG 
CTGAAGAGCA GCAATAACTC TATTCGAAAG TTGGAGATCA AAGAGGCTGC CTTAAAAGCC 
AAGGAGCTGC TGGACAAACA CTCCAATAAA CCTGTCGTGG TGGATTTGAT CCAGACTGAC 
TCTATTTCAG TTCTAATGAA GACTGTGGTC CAGCTGAGCA AGAGCACTCC AAAGTCCTTG 
GTCATGTTGT TCTCTCACTG GGAGTCATCA GGGAAGCTGC TCTGTGCCTG TCTAGTACCC 
AAAGGCTCTA CTGGTGGCTC GGCGATCGAA TGGGCTCTTT CTGTGTGTTC ACGCTTGGGT 
GGAAATGCAG AAGGTGCAAA GGACACCGCT AAAGGTGTGG GGAATGCAGT CAAAGTCGAG 
GATGTTGCAG AGATCCTGCA CTGGGCTGAA GAATTTGCAC ACAACAAAAT CAGAAACGTG 
CCCCGAACTG ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001332388.5
CDS	7..3021
Translation	MAARITVVKHVHCALLITKLSCLSSGIRRCTALSFSVYRREFTSKRVRRKFIDFFREGYGHRVVPSSSVRPRGDPSLLFVNAGMNQFKPILLGCADPRSEMVSYRRVVNSQKCVRAGGKHNDLQDVGKDVYHHTFFEMLGNWSFGDYFKVEACAMAWRLLTEEYGIPAQRLYVSYFSGDPANGLPADEETRDIWLSMGLRADHVLPFGMKDNFWEMGETGPCGPCTEIHYDHIGNRNAALLVNADSPDVVEIWNLVFMQYNRESNGSLRALPQCSVDTGMGLERLVTVLQGKRSNYDTDLFTPLLSAIHQCSKAPAYQGRTGAADVAQLDMAYRVVADHIRTICVCIADGVYPGMAGAELVLRRILRRAVRFSTEVLQAPEGALASLVPTVAHLLGDAYPELNTESDRIMDLINQNEAHFLSSLKQGRRVIDRTLSNMDENSSFFPASVAWSLYRNLGFPLDLIDLMLEEKGKIVDKKEMKVLEDEYEKLGLQSEEDEGEKLDQLDLHSLAELQRQRVPHTDDSPKYCYSVGSDGQYVFEPCKASVLALYCDGALVSEVRQGQHCAVILDQTSFYAEQGGQANDQGYFTKDGLQDVLFPVKCVRLAGGYVVHKVTAAETLRTGDQVHLHVDEAWRLGCMINHTATHVLNFALRELLGPIVSQRGSHCSANRLRFDFSVKGTLTVSELQKVEELVLNIIRQNADVHVEEVPLSSAKQIAGLRTVDELYPDPVRVVSVAVPVSDLLASERAEQTSVELCCGTHLLRTGAIRDFVVVSERQMMKGVCRIVAFTGDDAIKAREAGQALQEELESLEARMGLSNTLSIQEAKRLSKEVGHLINAVDITPMPQWKRLELQTRLKAMLKSSNNSIRKLEIKEAALKAKELLDKHSNKPVVVDLIQTDSISVLMKTVVQLSKSTPKSLVMLFSHWESSGKLLCACLVPKGSTGGSAIEWALSVCSRLGGNAEGAKDTAKGVGNAVKVEDVAEILHWAEEFAHNKIRNVPRTD

Target ORF information:

RefSeq Version	XM_001332352.7
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio alanyl-tRNA synthetase 2, mitochondrial (putative) (aars2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001332352.7

ORF Insert Sequence:

ATGGCCGCGC GCATTACAGT CGTGAAACAT GTTCACTGTG CACTTCTAAT TACAAAATTG 
AGCTGTTTGT CTTCGGGAAT AAGAAGATGC ACGGCTCTGT CATTCTCAGT ATATCGTAGG 
GAATTCACAT CTAAAAGAGT ACGGCGGAAG TTTATCGACT TTTTCAGGGA AGGTTATGGA 
CATCGGGTGG TTCCGTCATC CTCCGTACGC CCGAGAGGAG ACCCCAGTCT GCTATTCGTC 
AATGCAGGAA TGAACCAGTT TAAGCCGATT CTGCTGGGCT GTGCTGACCC TCGCAGTGAG 
ATGGTCTCGT ACCGGCGGGT GGTAAACAGT CAGAAGTGTG TCCGTGCTGG AGGCAAACAC 
AATGACCTAC AGGATGTGGG GAAGGACGTT TACCATCATA CCTTCTTCGA GATGCTGGGG 
AACTGGTCAT TCGGGGACTA CTTCAAGGTG GAAGCTTGTG CAATGGCCTG GCGTCTTTTA 
ACGGAAGAGT ATGGCATTCC TGCGCAGCGC CTGTATGTCT CGTACTTTTC TGGAGATCCA 
GCTAATGGTC TACCTGCTGA TGAGGAGACA CGGGACATCT GGCTCAGCAT GGGACTGAGA 
GCGGATCATG TGTTGCCATT TGGGATGAAG GATAACTTCT GGGAAATGGG AGAGACGGGC 
CCATGTGGCC CCTGCACGGA GATTCACTAC GATCACATTG GCAACAGGAA CGCTGCCTTA 
CTGGTCAATG CAGACAGTCC AGATGTAGTG GAAATCTGGA ACCTGGTATT CATGCAATAT 
AACAGGGAGA GCAATGGTAG TCTGCGAGCT CTTCCCCAGT GCAGTGTGGA TACTGGCATG 
GGTTTAGAGA GGCTGGTGAC GGTGCTGCAA GGCAAACGCT CCAACTACGA CACAGATCTC 
TTCACACCTC TATTATCTGC CATCCACCAG TGCTCTAAGG CTCCCGCATA TCAGGGCAGA 
ACCGGAGCGG CTGATGTGGC TCAGCTGGAC ATGGCCTATC GGGTCGTGGC GGATCACATC 
CGAACAATCT GTGTGTGTAT CGCTGACGGA GTTTACCCAG GCATGGCAGG CGCTGAGCTG 
GTGTTAAGAC GCATCTTAAG ACGTGCGGTT AGGTTTTCTA CAGAGGTGCT GCAGGCTCCT 
GAGGGTGCGC TGGCAAGTCT GGTGCCCACT GTAGCTCATC TACTGGGTGA TGCCTATCCT 
GAGCTGAACA CAGAGTCTGA TAGAATCATG GATCTCATTA ATCAGAATGA AGCCCACTTC 
CTTTCCTCGC TGAAACAGGG TAGGAGGGTG ATTGACAGGA CTCTCAGCAA CATGGACGAA 
AACTCTTCTT TTTTTCCAGC CTCGGTTGCC TGGTCCTTAT ATCGCAACCT GGGCTTTCCT 
CTGGATCTCA TTGACCTAAT GCTAGAGGAA AAAGGCAAGA TTGTTGATAA AAAGGAAATG 
AAGGTGCTGG AAGATGAATA TGAGAAGTTG GGTCTTCAGT CAGAGGAGGA TGAAGGTGAA 
AAGCTGGACC AGCTGGACCT TCATAGCCTG GCGGAGCTAC AACGGCAAAG AGTTCCTCAC 
ACAGACGACT CTCCAAAATA CTGTTACAGT GTGGGGTCCG ATGGACAATA CGTGTTTGAG 
CCCTGCAAAG CGTCTGTCCT GGCACTTTAC TGTGATGGGG CACTGGTTTC AGAGGTGCGT 
CAGGGTCAGC ACTGCGCTGT GATCCTGGAC CAGACCAGCT TTTATGCAGA GCAGGGCGGA 
CAGGCCAATG ACCAGGGTTA CTTCACCAAG GATGGGCTGC AGGATGTGTT GTTCCCTGTA 
AAGTGTGTCC GTCTCGCTGG GGGTTATGTG GTACACAAAG TCACAGCAGC AGAGACTTTG 
AGAACTGGAG ATCAAGTGCA TCTTCACGTG GATGAGGCTT GGCGGCTGGG CTGCATGATA 
AATCACACCG CCACCCATGT GCTGAACTTT GCTCTGCGAG AGTTGCTAGG TCCCATTGTC 
AGCCAACGAG GGTCTCATTG CAGTGCGAAT CGTCTACGCT TCGACTTCAG TGTCAAGGGC 
ACCCTGACTG TCTCTGAGCT GCAGAAAGTT GAGGAGCTGG TGCTCAATAT CATTCGTCAA 
AATGCAGATG TGCACGTAGA GGAGGTTCCT CTGTCGAGTG CTAAACAAAT TGCTGGCCTG 
AGGACTGTAG ATGAGCTCTA TCCAGACCCA GTGCGAGTGG TGTCCGTGGC TGTGCCTGTT 
TCGGATCTGC TTGCTTCAGA AAGAGCTGAG CAAACTTCAG TTGAGCTCTG CTGTGGCACG 
CATTTACTGA GGACGGGAGC AATTAGGGAC TTTGTTGTTG TATCTGAGCG ACAGATGATG 
AAAGGCGTTT GCCGTATTGT TGCCTTTACT GGAGATGACG CCATAAAGGC TAGAGAGGCA 
GGCCAAGCCT TGCAGGAGGA GTTGGAGTCT CTGGAGGCCC GGATGGGATT GTCAAACACA 
TTGTCTATTC AAGAAGCAAA GCGGCTTTCC AAAGAGGTTG GCCACCTAAT CAATGCAGTT 
GATATCACAC CTATGCCACA ATGGAAGAGA CTAGAGTTGC AGACTCGTCT GAAAGCGATG 
CTGAAGAGCA GCAATAACTC TATTCGAAAG TTGGAGATCA AAGAGGCTGC CTTAAAAGCC 
AAGGAGCTGC TGGACAAACA CTCCAATAAA CCTGTCGTGG TGGATTTGAT CCAGACTGAC 
TCTATTTCAG TTCTAATGAA GACTGTGGTC CAGCTGAGCA AGAGCACTCC AAAGTCCTTG 
GTCATGTTGT TCTCTCACTG GGAGTCATCA GGGAAGCTGC TCTGTGCCTG TCTAGTACCC 
AAAGGCTCTA CTGGTGGCTC GGCGATCGAA TGGGCTCTTT CTGTGTGTTC ACGCTTGGGT 
GGAAATGCAG AAGGTGCAAA GGACACCGCT AAAGGTGTGG GGAATGCAGT CAAAGTCGAG 
GATGTTGCAG AGATCCTGCA CTGGGCTGAA GAATTTGCAC ACAACAAAAT CAGAAACGTG 
CCCCGAACTG ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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aars2 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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