cep170b cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	cep170b
Entrez Gene ID	792564
Full Name	centrosomal protein 170B
Synonyms	si:ch73-160l8.1
General protein information	Preferred Names centrosomal protein 170B Names centrosomal protein of 170 kDa protein B
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_017351731.1	XP_017207220.1	centrosomal protein of 170 kDa protein B
XM_021467254.1	XP_021322929.1	centrosomal protein of 170 kDa protein B isoform X2
XM_017351731.2	XP_017207220.2	centrosomal protein of 170 kDa protein B isoform X1
XM_021467256.1	XP_021322931.1	centrosomal protein of 170 kDa protein B isoform X4
XM_021467255.1	XP_021322930.1	centrosomal protein of 170 kDa protein B isoform X3

Pathways from BioSystems
Gene Ontology

The following cep170b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the cep170b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa149605	XM_021467255.1 Latest version!	Danio rerio centrosomal protein 170B (cep170b), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
ODa149606	XM_021467256.1 Latest version!	Danio rerio centrosomal protein 170B (cep170b), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
ODa149603	XM_017351731.2 Latest version!	Danio rerio centrosomal protein 170B (cep170b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
ODa149604	XM_021467254.1 Latest version!	Danio rerio centrosomal protein 170B (cep170b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
ODa55659	XM_017351731.1 Latest version!	Danio rerio centrosomal protein 170B (cep170b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

cep170b ( XM_021467255.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021322930.1 Danio rerio centrosomal protein 170B (cep170b), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa149605
Clone ID Related Accession (Same CDS sequence)	XM_021467255.1
Accession Version	XM_021467255.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4407bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	centrosomal protein of 170 kDa protein B isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.7) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGTGA CGTCGTGGTT TCTGGTGAGC AGCTCTGGCA CGCGCCACCG TTTGCCCCGA 
GAGATGATCT TTGTGGGTAG AGAAGACTGT GAACTCATGC TACAGTCGCG CAGTGTGGAC 
AAACAGCACG CTGTGATCAA CTATGATGCA TCTACAGATG AACATTTGGT CAAAGACTTG 
GGCAGCCTCA ACGGGACCTT CGTAAATGAC CTTCGCATCC CCGACCAGAC GTACATCATC 
CTGAAGTTGT CAGATGTGAT CCGCTTTGGT TATGATTCAC ACATATATAT CCTGGAGAGA 
AGCCAACACA AAGTGCCTGA GGAGGCTCTC AAGCATGAAA AATACACCAG TCAGCTTCAG 
CTGGAGACGC TGGAAAACAA GAAGCAGCTT CAGACAGGTG ACAGACGGAG AAACTCAGAC 
TCCACACGGG TCAAATCTGA AAAATCAGAG CGGAAAAACA TCACAGATGC ACCGATTTCC 
AGACCCACAC CTCTTTATGG TCAGCCGTCC TGGTGGGGTG AAGACGATGA AGATAAAGCT 
CAGCGCTGTG ATCCGAGCTC TCAGGAGCAA CATACAGAAA ACACGAAGGA AGGGACCAAA 
CATGATCTCA GCATTTCTCC AGACGATCTT CACGATGGCC AGAGCAAATC CAGCTTCATT 
CACAAAAAGG AGCCCAGTTA TTTTGAGATC CCTACTAAGG AGAACCATCC AAAGGCCAAA 
AGCACATTGA CTGAGATGCA GGAGATTCCC ACAAAAGACC CCGACCACAA GCCTCCGTCC 
GTCTCTCCGG TGGTGCAGGG TCACGCCTCC TTCACCATTG AGTTTGACGA GCAAACGCCT 
GGAAAGATGA AGATTAAGGA CCACATCTCT AAAATGTCCA TCCGCCAGCG CAGAAACCCC 
AGCAAGGAGT CTATAAGCCG CCTGACGGAG GTGATGTCTG TGGAGAATAA AGTGGCTGAT 
TGGTTGGTGC AGAGCGACAC GTCTTTGAGG AGTCTTGAGG AGCCCAGATC AGATCAGCTG 
CTCAACAAGA CCTTCAGAGA TGCACACCAT AATGAAGATC CAGATGTGGA AGAACAGGAG 
GAAATTCTGT CAGTCTCACA CTCAGCTGCA CAAAAAACCC CACCACAACA AGAGCCTGTA 
GATTATCCAT CCAATCAAAC GGCAGATTCA AAGAACCAAT GCAGAACGGA CCCCAAACAG 
GCTTACGTGA TTGAATTCTT TGATGATGCT CAACGCAAAA AGCGCTCGCA GTCTTTCACC 
AACAACATCT CCTCTCCAGA CTTCCAGTCT GCGCTGAAAA ACAAGTTGGA CAAACGAAAA 
GGCACAACTT TGTCATCCGG ACACAATCCA GCAACACAAC AGTTCACGAT TCCCCTCAAA 
GATTCAGATA GTCCACAGAG AGCTGAATCT CTGCGTCGCG AGACGAGCGA AATCCGAATG 
AGCACTTCTG AATTCAGCTC TCGCTCTGTG ACAAAACCGT TCGGCAGCAT CGGCCGCAGG 
TCCAAAATCT CGCAGGATTT CGCAGCGGAG TTGCTTCGGG TGTCAAAACA CAGTGCTGCG 
TCTTCATGGG ACTCCAATAC AACTTGGGTG AAAACGTCTT CGGCATTAAC GACAAATAAT 
GTGGTGCAGG AGAGTCTTTC TCAATCGCAG AATCACATGA TCAGATCTGA GCGAGTGGAG 
GATGGTGATA ATACTGAGGG CAAACCTCCC AAAGGGCAGG AGGAGGAGGA CAGTCTGAGT 
GATGCAGGAA CATACACTAT TGAAGCTGAA GGGCCGGATA GAGACGTTGC GGAGGCGCGG 
AGCTTGATTG ATCAGGTGTT TGGTGTTGGA GAAAACAGCC AAACCACAGC AGCATCAGCA 
GCAGATAAGG CTGTAGTTGG TGACTCTGAG GCTCATGATA GCCTTCTTGT CAATGAGAAT 
AGTTCAGGCC TGATAATGGG TCAGTTCCCA TCAGAACATA GTGCCACAGC CAAGAGTCCT 
GCGCAGTTAC AGTCAATGAC GTCATTAACA CCTGGAGGCA CTCGATGGGT TTCTCGCTGG 
GCCAGTTTGG CTGATACCTA TTCAGACTCT GGTCCCGCTT CAGGAATATT TGATGTCCCA 
ACACAGGTTG ATCTTCCCTC CGAAGCTGGT GAAAGGATAA ACATGCTTAA CCGGAGCGCT 
GACTACTCTG AAAATAGCCA AAACAGCAGA TCACGACGAG TTCTGCCTCA GGTTCCTTTA 
AAGGAGAAGA TTGAGACACC TCCACCCATG ATACACATCC AACCTGATCC GTACCTGTCC 
ACTGTGGAGA AGAGTTCACA AGTTCAAAAG CACAAGTGGG ATCCACAGAA ACTCAATGTG 
CAGGATGATC TGGATCCAGA CAGCCTTAGC GATGCTAGCA AGTCTGATGA CGGATCTGTC 
ATTGAACAGT GCAGGAAGAC TCCCGAAGTG AACCAGAAGA CATGGAGTCG GCCTTCAAAA 
ACACCATCCA ATCAAGACGA GGCAAAAGAC CAGGATACAC CTCAGAAGTT CTCCACTGCT 
ACGTTAACCC GACAGTATGG GAAACCAAGC AAGAGCAATG GTGCACCACC CAATATTGGT 
CAGCCAAGCA AAAATGACTC TTTTGGAGGA GACAGTTTAG TGTCTATTGT TAGACAAGAA 
AGTTACACCA AAGAAAGAGC GAGTGATGAC ATCCAGTTGT CCAGATTGCC CCAGATATCG 
AGTCAGCCCA TGAGCAAAGA TGTTTTTAAG GGTGTAAACA GTCAAGATAC TCATTCATAC 
CTTAAAGACA CTGAGGATGT TCTTGCATCT CTGGAAGCCA AGATTCTGGA GCAACAAGTA 
AACAACGGTC GCTCACATCC GGAGGACTCT TTCTCTGGAG AATCCGACAC TTCTAGTACT 
GTCAGTGGCA AAAACCCTTG CGTTTCTGTT CCCAAGAAGC CTATAGTACT CAAAGGATTC 
TTAAAAGGTA CCGGTCAGTA TACCAGAGAA CCTAAAACCT CAGAAGATCA GTCTGGGATG 
AAGGAGAAGT CAGATCCAGG CAAGCGTTTT CAGCTCCGTC GAAATCATAG TTCTCAAGAC 
TTTGGAAATG AACAGCAAAG TCCTGCTACA CGGCAGTTTC CTGACACTGT CTCAGACCAG 
GAATCAAGTT CTTTACCTTA TAAAAAGTAC ACTATTCCTC TTCAGAAGGA AGGTACGAGC 
AAATCCAAAC CTACCGTAAC TCGCTCCAAT AGTCTTTCCG CTCCAAGACC AACAAGAGCG 
TCAATGCTTC GACGTGGACG TTTGGGTGAA GCCTCTGATA ATGAAGGTAC CGAAACCGAT 
CGAACGTCTC AGAATTCCGA CGTCAATCCT TCTGCAAACA ATCGAGTGGC TCAAACAAGC 
AAGAAACTTT CTCGATTGGA CATTTTGGCT CTGCCTAGGA AAAGGACGGG ATCTTTTAAC 
ACCCCGAGTG ACACAGAGTC TTCTTCTGCG AGCCGGACGA GCTTCTCGAA CCGCAGCACA 
GAGTCGAGCA GCTCCGTGCG GAAAGCATCA GTTCCGGATC CCAGGGCTCT TCTGCGTAAG 
GTTACAAATG CCATCAGCAG GCAGCCGATC ACTCGCGGAC GCTCGAGCAG TGCCAAATAC 
GCCAGCAGCA CCGCCAGAGC GCAGCAGAGC CAGCCGGCAG GTCCGGTGAG AGCCATGCCC 
AGATCCAGAG ACTCTGAGGG GGAAGGACAG GAGAGCGACG CCTTCCAGAA CTGGACGTCT 
CACAGTGCCG AGATCGCCAG ATTGAGTCAG GATCTCGCTA AAGATTTGGC TATTTTGGCC 
CAAGAGATTC ATGATGTCGC AGGAGACACT GAGACTCAGA GCGGATCACA AGAGCAGACG 
GAGCCCGCCA TATCCACACA AGAGGAGTTG CGGCATCAGA TTCCTGAATC CAGTCTGAAA 
GTGCAGAAAG AGTCTCCTGG AGCAGTCGAA GAGTCTGAAC ACACCGCTGA AGATGAGAAT 
CAAGCCAATA GGAGACAAGA CGAGGCTGCT GTGAACAGTG TGCTCCTCAA TCCTGTGTCT 
CAGCTTTCAC TCGCCATCAG GGAAAACACC GAACAGCTCA CACACAAAAT CAAGGTTTTG 
TTTCATAATC GGAGAGATGT TTTGGGTGAA ATCGAGGCAA AGATCAGTGC AGAGGATGAT 
TCTCTATCTA TGAAAACCTC AAACAAGGAA GTGGCGTCGA TCCTGAAAGA GCTGAGGAGA 
GTCCAGAAGC AGCTGGAAGT GATCAACAGC ATCATGGATC CGCAGGGGAA CCCGGAGCTG 
ATGCGGACGT TCTCCTCATC TGCAGCTGCA GCGCGTGGAT CCAGATCTGC TCTGCGGGAC 
AACAGATCTG CAGGAGGAGG AGCAGGAGCA ACACCCAGAG CCAGACGCAT GTTCAGGAGC 
ACAGACAGAG ACGGCTCGAT GGTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021322930.1
CDS	295..4701
Translation	MSVTSWFLVSSSGTRHRLPREMIFVGREDCELMLQSRSVDKQHAVINYDASTDEHLVKDLGSLNGTFVNDLRIPDQTYIILKLSDVIRFGYDSHIYILERSQHKVPEEALKHEKYTSQLQLETLENKKQLQTGDRRRNSDSTRVKSEKSERKNITDAPISRPTPLYGQPSWWGEDDEDKAQRCDPSSQEQHTENTKEGTKHDLSISPDDLHDGQSKSSFIHKKEPSYFEIPTKENHPKAKSTLTEMQEIPTKDPDHKPPSVSPVVQGHASFTIEFDEQTPGKMKIKDHISKMSIRQRRNPSKESISRLTEVMSVENKVADWLVQSDTSLRSLEEPRSDQLLNKTFRDAHHNEDPDVEEQEEILSVSHSAAQKTPPQQEPVDYPSNQTADSKNQCRTDPKQAYVIEFFDDAQRKKRSQSFTNNISSPDFQSALKNKLDKRKGTTLSSGHNPATQQFTIPLKDSDSPQRAESLRRETSEIRMSTSEFSSRSVTKPFGSIGRRSKISQDFAAELLRVSKHSAASSWDSNTTWVKTSSALTTNNVVQESLSQSQNHMIRSERVEDGDNTEGKPPKGQEEEDSLSDAGTYTIEAEGPDRDVAEARSLIDQVFGVGENSQTTAASAADKAVVGDSEAHDSLLVNENSSGLIMGQFPSEHSATAKSPAQLQSMTSLTPGGTRWVSRWASLADTYSDSGPASGIFDVPTQVDLPSEAGERINMLNRSADYSENSQNSRSRRVLPQVPLKEKIETPPPMIHIQPDPYLSTVEKSSQVQKHKWDPQKLNVQDDLDPDSLSDASKSDDGSVIEQCRKTPEVNQKTWSRPSKTPSNQDEAKDQDTPQKFSTATLTRQYGKPSKSNGAPPNIGQPSKNDSFGGDSLVSIVRQESYTKERASDDIQLSRLPQISSQPMSKDVFKGVNSQDTHSYLKDTEDVLASLEAKILEQQVNNGRSHPEDSFSGESDTSSTVSGKNPCVSVPKKPIVLKGFLKGTGQYTREPKTSEDQSGMKEKSDPGKRFQLRRNHSSQDFGNEQQSPATRQFPDTVSDQESSSLPYKKYTIPLQKEGTSKSKPTVTRSNSLSAPRPTRASMLRRGRLGEASDNEGTETDRTSQNSDVNPSANNRVAQTSKKLSRLDILALPRKRTGSFNTPSDTESSSASRTSFSNRSTESSSSVRKASVPDPRALLRKVTNAISRQPITRGRSSSAKYASSTARAQQSQPAGPVRAMPRSRDSEGEGQESDAFQNWTSHSAEIARLSQDLAKDLAILAQEIHDVAGDTETQSGSQEQTEPAISTQEELRHQIPESSLKVQKESPGAVEESEHTAEDENQANRRQDEAAVNSVLLNPVSQLSLAIRENTEQLTHKIKVLFHNRRDVLGEIEAKISAEDDSLSMKTSNKEVASILKELRRVQKQLEVINSIMDPQGNPELMRTFSSSAAAARGSRSALRDNRSAGGGAGATPRARRMFRSTDRDGSMV

Target ORF information:

RefSeq Version	XM_021467255.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio centrosomal protein 170B (cep170b), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021467255.1

ORF Insert Sequence:

ATGAGCGTGA CGTCGTGGTT TCTGGTGAGC AGCTCTGGCA CGCGCCACCG TTTGCCCCGA 
GAGATGATCT TTGTGGGTAG AGAAGACTGT GAACTCATGC TACAGTCGCG CAGTGTGGAC 
AAACAGCACG CTGTGATCAA CTATGATGCA TCTACAGATG AACATTTGGT CAAAGACTTG 
GGCAGCCTCA ACGGGACCTT CGTAAATGAC CTTCGCATCC CCGACCAGAC GTACATCATC 
CTGAAGTTGT CAGATGTGAT CCGCTTTGGT TATGATTCAC ACATATATAT CCTGGAGAGA 
AGCCAACACA AAGTGCCTGA GGAGGCTCTC AAGCATGAAA AATACACCAG TCAGCTTCAG 
CTGGAGACGC TGGAAAACAA GAAGCAGCTT CAGACAGGTG ACAGACGGAG AAACTCAGAC 
TCCACACGGG TCAAATCTGA AAAATCAGAG CGGAAAAACA TCACAGATGC ACCGATTTCC 
AGACCCACAC CTCTTTATGG TCAGCCGTCC TGGTGGGGTG AAGACGATGA AGATAAAGCT 
CAGCGCTGTG ATCCGAGCTC TCAGGAGCAA CATACAGAAA ACACGAAGGA AGGGACCAAA 
CATGATCTCA GCATTTCTCC AGACGATCTT CACGATGGCC AGAGCAAATC CAGCTTCATT 
CACAAAAAGG AGCCCAGTTA TTTTGAGATC CCTACTAAGG AGAACCATCC AAAGGCCAAA 
AGCACATTGA CTGAGATGCA GGAGATTCCC ACAAAAGACC CCGACCACAA GCCTCCGTCC 
GTCTCTCCGG TGGTGCAGGG TCACGCCTCC TTCACCATTG AGTTTGACGA GCAAACGCCT 
GGAAAGATGA AGATTAAGGA CCACATCTCT AAAATGTCCA TCCGCCAGCG CAGAAACCCC 
AGCAAGGAGT CTATAAGCCG CCTGACGGAG GTGATGTCTG TGGAGAATAA AGTGGCTGAT 
TGGTTGGTGC AGAGCGACAC GTCTTTGAGG AGTCTTGAGG AGCCCAGATC AGATCAGCTG 
CTCAACAAGA CCTTCAGAGA TGCACACCAT AATGAAGATC CAGATGTGGA AGAACAGGAG 
GAAATTCTGT CAGTCTCACA CTCAGCTGCA CAAAAAACCC CACCACAACA AGAGCCTGTA 
GATTATCCAT CCAATCAAAC GGCAGATTCA AAGAACCAAT GCAGAACGGA CCCCAAACAG 
GCTTACGTGA TTGAATTCTT TGATGATGCT CAACGCAAAA AGCGCTCGCA GTCTTTCACC 
AACAACATCT CCTCTCCAGA CTTCCAGTCT GCGCTGAAAA ACAAGTTGGA CAAACGAAAA 
GGCACAACTT TGTCATCCGG ACACAATCCA GCAACACAAC AGTTCACGAT TCCCCTCAAA 
GATTCAGATA GTCCACAGAG AGCTGAATCT CTGCGTCGCG AGACGAGCGA AATCCGAATG 
AGCACTTCTG AATTCAGCTC TCGCTCTGTG ACAAAACCGT TCGGCAGCAT CGGCCGCAGG 
TCCAAAATCT CGCAGGATTT CGCAGCGGAG TTGCTTCGGG TGTCAAAACA CAGTGCTGCG 
TCTTCATGGG ACTCCAATAC AACTTGGGTG AAAACGTCTT CGGCATTAAC GACAAATAAT 
GTGGTGCAGG AGAGTCTTTC TCAATCGCAG AATCACATGA TCAGATCTGA GCGAGTGGAG 
GATGGTGATA ATACTGAGGG CAAACCTCCC AAAGGGCAGG AGGAGGAGGA CAGTCTGAGT 
GATGCAGGAA CATACACTAT TGAAGCTGAA GGGCCGGATA GAGACGTTGC GGAGGCGCGG 
AGCTTGATTG ATCAGGTGTT TGGTGTTGGA GAAAACAGCC AAACCACAGC AGCATCAGCA 
GCAGATAAGG CTGTAGTTGG TGACTCTGAG GCTCATGATA GCCTTCTTGT CAATGAGAAT 
AGTTCAGGCC TGATAATGGG TCAGTTCCCA TCAGAACATA GTGCCACAGC CAAGAGTCCT 
GCGCAGTTAC AGTCAATGAC GTCATTAACA CCTGGAGGCA CTCGATGGGT TTCTCGCTGG 
GCCAGTTTGG CTGATACCTA TTCAGACTCT GGTCCCGCTT CAGGAATATT TGATGTCCCA 
ACACAGGTTG ATCTTCCCTC CGAAGCTGGT GAAAGGATAA ACATGCTTAA CCGGAGCGCT 
GACTACTCTG AAAATAGCCA AAACAGCAGA TCACGACGAG TTCTGCCTCA GGTTCCTTTA 
AAGGAGAAGA TTGAGACACC TCCACCCATG ATACACATCC AACCTGATCC GTACCTGTCC 
ACTGTGGAGA AGAGTTCACA AGTTCAAAAG CACAAGTGGG ATCCACAGAA ACTCAATGTG 
CAGGATGATC TGGATCCAGA CAGCCTTAGC GATGCTAGCA AGTCTGATGA CGGATCTGTC 
ATTGAACAGT GCAGGAAGAC TCCCGAAGTG AACCAGAAGA CATGGAGTCG GCCTTCAAAA 
ACACCATCCA ATCAAGACGA GGCAAAAGAC CAGGATACAC CTCAGAAGTT CTCCACTGCT 
ACGTTAACCC GACAGTATGG GAAACCAAGC AAGAGCAATG GTGCACCACC CAATATTGGT 
CAGCCAAGCA AAAATGACTC TTTTGGAGGA GACAGTTTAG TGTCTATTGT TAGACAAGAA 
AGTTACACCA AAGAAAGAGC GAGTGATGAC ATCCAGTTGT CCAGATTGCC CCAGATATCG 
AGTCAGCCCA TGAGCAAAGA TGTTTTTAAG GGTGTAAACA GTCAAGATAC TCATTCATAC 
CTTAAAGACA CTGAGGATGT TCTTGCATCT CTGGAAGCCA AGATTCTGGA GCAACAAGTA 
AACAACGGTC GCTCACATCC GGAGGACTCT TTCTCTGGAG AATCCGACAC TTCTAGTACT 
GTCAGTGGCA AAAACCCTTG CGTTTCTGTT CCCAAGAAGC CTATAGTACT CAAAGGATTC 
TTAAAAGGTA CCGGTCAGTA TACCAGAGAA CCTAAAACCT CAGAAGATCA GTCTGGGATG 
AAGGAGAAGT CAGATCCAGG CAAGCGTTTT CAGCTCCGTC GAAATCATAG TTCTCAAGAC 
TTTGGAAATG AACAGCAAAG TCCTGCTACA CGGCAGTTTC CTGACACTGT CTCAGACCAG 
GAATCAAGTT CTTTACCTTA TAAAAAGTAC ACTATTCCTC TTCAGAAGGA AGGTACGAGC 
AAATCCAAAC CTACCGTAAC TCGCTCCAAT AGTCTTTCCG CTCCAAGACC AACAAGAGCG 
TCAATGCTTC GACGTGGACG TTTGGGTGAA GCCTCTGATA ATGAAGGTAC CGAAACCGAT 
CGAACGTCTC AGAATTCCGA CGTCAATCCT TCTGCAAACA ATCGAGTGGC TCAAACAAGC 
AAGAAACTTT CTCGATTGGA CATTTTGGCT CTGCCTAGGA AAAGGACGGG ATCTTTTAAC 
ACCCCGAGTG ACACAGAGTC TTCTTCTGCG AGCCGGACGA GCTTCTCGAA CCGCAGCACA 
GAGTCGAGCA GCTCCGTGCG GAAAGCATCA GTTCCGGATC CCAGGGCTCT TCTGCGTAAG 
GTTACAAATG CCATCAGCAG GCAGCCGATC ACTCGCGGAC GCTCGAGCAG TGCCAAATAC 
GCCAGCAGCA CCGCCAGAGC GCAGCAGAGC CAGCCGGCAG GTCCGGTGAG AGCCATGCCC 
AGATCCAGAG ACTCTGAGGG GGAAGGACAG GAGAGCGACG CCTTCCAGAA CTGGACGTCT 
CACAGTGCCG AGATCGCCAG ATTGAGTCAG GATCTCGCTA AAGATTTGGC TATTTTGGCC 
CAAGAGATTC ATGATGTCGC AGGAGACACT GAGACTCAGA GCGGATCACA AGAGCAGACG 
GAGCCCGCCA TATCCACACA AGAGGAGTTG CGGCATCAGA TTCCTGAATC CAGTCTGAAA 
GTGCAGAAAG AGTCTCCTGG AGCAGTCGAA GAGTCTGAAC ACACCGCTGA AGATGAGAAT 
CAAGCCAATA GGAGACAAGA CGAGGCTGCT GTGAACAGTG TGCTCCTCAA TCCTGTGTCT 
CAGCTTTCAC TCGCCATCAG GGAAAACACC GAACAGCTCA CACACAAAAT CAAGGTTTTG 
TTTCATAATC GGAGAGATGT TTTGGGTGAA ATCGAGGCAA AGATCAGTGC AGAGGATGAT 
TCTCTATCTA TGAAAACCTC AAACAAGGAA GTGGCGTCGA TCCTGAAAGA GCTGAGGAGA 
GTCCAGAAGC AGCTGGAAGT GATCAACAGC ATCATGGATC CGCAGGGGAA CCCGGAGCTG 
ATGCGGACGT TCTCCTCATC TGCAGCTGCA GCGCGTGGAT CCAGATCTGC TCTGCGGGAC 
AACAGATCTG CAGGAGGAGG AGCAGGAGCA ACACCCAGAG CCAGACGCAT GTTCAGGAGC 
ACAGACAGAG ACGGCTCGAT GGTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cep170b ( XM_021467256.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021322931.1 Danio rerio centrosomal protein 170B (cep170b), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ODa149606
Clone ID Related Accession (Same CDS sequence)	XM_021467256.1
Accession Version	XM_021467256.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4338bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	centrosomal protein of 170 kDa protein B isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGTGA CGTCGTGGTT TCTGGTGAGC AGCTCTGGCA CGCGCCACCG TTTGCCCCGA 
GAGATGATCT TTGTGGGTAG AGAAGACTGT GAACTCATGC TACAGTCGCG CAGTGTGGAC 
AAACAGCACG CTGTGATCAA CTATGATGCA TCTACAGATG AACATTTGGT CAAAGACTTG 
GGCAGCCTCA ACGGGACCTT CGTAAATGAC CTTCGCATCC CCGACCAGAC GTACATCATC 
CTGAAGTTGT CAGATGTGAT CCGCTTTGGT TATGATTCAC ACATATATAT CCTGGAGAGA 
AGCCAACACA AAGTGCCTGA GGAGGCTCTC AAGCATGAAA AATACACCAG TCAGCTTCAG 
CTGGAGACGC TGGAAAACAA GAAGCAGCTT CAGACAGGTG ACAGACGGAG AAACTCAGAC 
TCCACACGGG TCAAATCTGA AAAATCAGAG CGGAAAAACA TCACAGATGC ACCGATTTCC 
AGACCCACAC CTCTTTATGG TCAGCCGTCC TGGTGGGGTG AAGACGATGA AGATAAAGCT 
CAGCGCTGTG ATCCGAGCTC TCAGGAGCAA CATACAGAAA ACACGAAGGA AGGGACCAAA 
CATGATCTCA GCATTTCTCC AGACGATCTT CACGATGGCC AGAGCAAATC CAGCTTCATT 
CACAAAAAGG AGCCCAGTTA TTTTGAGATC CCTACTAAGG AGAACCATCC AAAGGCCAAA 
AGCACATTGA CTGAGATGCA GGAGATTCCC ACAAAAGACC CCGACCACAA GCCTCCGTCC 
GTCTCTCCGG TGGTGCAGGG TCACGCCTCC TTCACCATTG AGTTTGACGA GCAAACGCCT 
GGAAAGATGA AGATTAAGGA CCACATCTCT AAAATGTCCA TCCGCCAGCG CAGAAACCCC 
AGCAAGGAGT CTATAAGCCG CCTGACGGAG GTGATGTCTG TGGAGAATAA AGTGGCTGAT 
TGGTTGGTGC AGAGCGACAC GTCTTTGAGG AGTCTTGAGG AGCCCAGATC AGATCAGCTG 
CTCAACAAGA CCTTCAGAGA TGCACACCAT AATGAAGATC CAGATGTGGA AGAACAGGAG 
GAAATTCTGT CAGTCTCACA CTCAGCTGCA CAAAAAACCC CACCACAACA AGAGCCTGTA 
GATTATCCAT CCAATCAAAC GGCAGATTCA AAGAACCAAT GCAGAACGGA CCCCAAACAG 
GCTTACGTGA TTGAATTCTT TGATGATGCT CAACGCAAAA AGCGCTCGCA GTCTTTCACC 
AACAACATCT CCTCTCCAGA CTTCCAGTCT GCGCTGAAAA ACAAGTTGGA CAAACGAAAA 
GGCACAACTT TGTCATCCGG ACACAATCCA GCAACACAAC AGTTCACGAT TCCCCTCAAA 
GATTCAGATA GTCCACAGAG AGCTGAATCT CTGCGTCGCG AGACGAGCGA AATCCGAATG 
AGCACTTCTG AATTCAGCTC TCGCTCTGTG ACAAAACCGT TCGGCAGCAT CGGCCGCAGG 
TCCAAAATCT CGCAGGATTT CGCAGCGGAG TTGCTTCGGG TGTCAAAACA CAGTGCTGCG 
TCTTCATGGG ACTCCAATAC AACTTGGGTG AAAACGTCTT CGGCATTAAC GACAAATAAT 
GTGGTGCAGG AGAGTCTTTC TCAATCGCAG AATCACATGA TCAGATCTGA GCGAGTGGAG 
GATGGTGATA ATACTGAGGG CAAACCTCCC AAAGGGCAGG AGGAGGAGGA CAGTCTGAGT 
GATGCAGGAA CATACACTAT TGAAGCTGAA GGGCCGGATA GAGACGTTGC GGAGGCGCGG 
AGCTTGATTG ATCAGTTACA GTCAATGACG TCATTAACAC CTGGAGGCAC TCGATGGGTT 
TCTCGCTGGG CCAGTTTGGC TGATACCTAT TCAGACTCTG GTCCCGCTTC AGGAATATTT 
GATGTCCCAA CACAGGTTGA TCTTCCCTCC GAAGCTGGTG AAAGGATAAA CATGCTTAAC 
CGGAGCGCTG ACTACTCTGA AAATAGCCAA AACAGCAGAT CACGACGAGT TCTGCCTCAG 
GTTCCTTTAA AGGAGAAGAT TGAGACACCT CCACCCATGA TACACATCCA ACCTGATCCG 
TACCTGTCCA CTGTGGAGAA GAGTTCACAA GTTCAAAAGC ACAAGTGGGA TCCACAGAAA 
CTCAATGTGC AGGATGATCT GGATCCAGAC AGCCTTAGCG ATGCTAGCAA GTCTGATGAC 
GGATCTGTCA TTGAACAGTG CAGGAAGACT CCCGAAGTGA ACCAGAAGAC ATGGAGTCGG 
CCTTCAAAAA CACCATCCAA TCAAGACGAG GCAAAAGACC AGGATACACC TCAGAAGTTC 
TCCACTGCTA CGTTAACCCG ACAGTATGGG AAACCAAGCA AGAGCAATGG TGCACCACCC 
AATATTGGTC AGCCAAGCAA AAATGACTCT TTTGGAGGAG ACAGTTTAGT GTCTATTGTT 
AGACAAGAAA GTTACACCAA AGAAAGAGCG AGTGATGACA TCCAGTTGTC CAGATTGCCC 
CAGATATCGA GTCAGCCCAT GAGCAAAGAT GTTTTTAAGG GTGTAAACAG TCAAGATACT 
CATTCATACC TTAAAGACAC TGAGGATGTT CTTGCATCTC TGGAAGCCAA GATTCTGGAG 
CAACAAGTAA ACAACGGTCG CTCACATCCG GAGGACTCTT TCTCTGGAGA ATCCGACACT 
TCTAGTACTG TCAGTGGCAA AAACCCTTGC GTTTCTGTTC CCAAGAAGCC TATAGTACTC 
AAAGGATTCT TAAAAGGTAC CGGTCAGTAT ACCAGAGAAC CTAAAACCTC AGAAGATCAG 
TCTGGGATGA AGGAGAAGTC AGATCCAGGC AAGCGTTTTC AGCTCCGTCG AAATCATAGT 
TCTCAAGACT TTGGAAATGA ACAGCAAAGT CCTGCTACAC GGCAGTTTCC TGACACTGTC 
TCAGACCAGG AATCAAGTTC TTTACCTTAT AAAAAGTACA CTATTCCTCT TCAGAAGGAA 
GGTACGAGCA AATCCAAACC TACCGTAACT CGCTCCAATA GTCTTTCCGC TCCAAGACCA 
ACAAGAGCGT CAATGCTTCG ACGTGGACGT TTGGGTGAAG CCTCTGATAA TGAAGGTACC 
GAAACCGATC GAACGTCTCA GAATTCCGAC GTCAATCCTT CTGCAAACAA TCGAGTGGCT 
CAAACAAGCA AGAAACTTTC TCGATTGGAC ATTTTGGCTC TGCCTAGGAA AAGGACGGGA 
TCTTTTAACA CCCCGAGTGA CACAGAGTCT TCTTCTGCGA GCCGGACGAG CTTCTCGAAC 
CGCAGCACAG AGTCGAGCAG CTCCGTGCGG AAAGCATCAG TTCCGGATCC CAGGGCTCTT 
CTGCGTAAGG TTACAAATGC CATCAGCAGG CAGCCGATCA CTCGCGGACG CTCGAGCAGT 
GCCAAATACG CCAGCAGCAC CGCCAGCTCC AGGAGGCGTC AGAAGGGTTC AGACTACGCC 
TCCACCTCGG AGGACGAGTA CGAGTCGAAC CAAAGCACTC CTAAACACAA ACGCTCCCAT 
CATTCAGGAG CGCAGCAGAG CCAGCCGGCA GGTCCGGTGA GAGCCATGCC CAGATCCAGA 
GACTCTGAGG GGGAAGGACA GGAGAGCGAC GCCTTCCAGA ACTGGACGTC TCACAGTGCC 
GAGATCGCCA GATTGAGTCA GGATCTCGCT AAAGATTTGG CTATTTTGGC CCAAGAGATT 
CATGATGTCG CAGGAGACAC TGAGACTCAG AGCGGATCAC AAGAGCAGAC GGAGCCCGCC 
ATATCCACAC AAGAGGAGTT GCGGCATCAG ATTCCTGAAT CCAGTCTGAA AGTGCAGAAA 
GAGTCTCCTG GAGCAGTCGA AGAGTCTGAA CACACCGCTG AAGATGAGAA TCAAGCCAAT 
AGGAGACAAG ACGAGGCTGC TGTGAACAGT GTGCTCCTCA ATCCTGTGTC TCAGCTTTCA 
CTCGCCATCA GGGAAAACAC CGAACAGCTC ACACACAAAA TCAAGGTTTT GTTTCATAAT 
CGGAGAGATG TTTTGGGTGA AATCGAGGCA AAGATCAGTG CAGAGGATGA TTCTCTATCT 
ATGAAAACCT CAAACAAGGA AGTGGCGTCG ATCCTGAAAG AGCTGAGGAG AGTCCAGAAG 
CAGCTGGAAG TGATCAACAG CATCATGGAT CCGCAGGGGA ACCCGGAGCT GATGCGGACG 
TTCTCCTCAT CTGCAGCTGC AGCGCGTGGA TCCAGATCTG CTCTGCGGGA CAACAGATCT 
GCAGGAGGAG GAGCAGGAGC AACACCCAGA GCCAGACGCA TGTTCAGGAG CACAGACAGA 
GACGGCTCGA TGGTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021322931.1
CDS	294..4631
Translation	MSVTSWFLVSSSGTRHRLPREMIFVGREDCELMLQSRSVDKQHAVINYDASTDEHLVKDLGSLNGTFVNDLRIPDQTYIILKLSDVIRFGYDSHIYILERSQHKVPEEALKHEKYTSQLQLETLENKKQLQTGDRRRNSDSTRVKSEKSERKNITDAPISRPTPLYGQPSWWGEDDEDKAQRCDPSSQEQHTENTKEGTKHDLSISPDDLHDGQSKSSFIHKKEPSYFEIPTKENHPKAKSTLTEMQEIPTKDPDHKPPSVSPVVQGHASFTIEFDEQTPGKMKIKDHISKMSIRQRRNPSKESISRLTEVMSVENKVADWLVQSDTSLRSLEEPRSDQLLNKTFRDAHHNEDPDVEEQEEILSVSHSAAQKTPPQQEPVDYPSNQTADSKNQCRTDPKQAYVIEFFDDAQRKKRSQSFTNNISSPDFQSALKNKLDKRKGTTLSSGHNPATQQFTIPLKDSDSPQRAESLRRETSEIRMSTSEFSSRSVTKPFGSIGRRSKISQDFAAELLRVSKHSAASSWDSNTTWVKTSSALTTNNVVQESLSQSQNHMIRSERVEDGDNTEGKPPKGQEEEDSLSDAGTYTIEAEGPDRDVAEARSLIDQLQSMTSLTPGGTRWVSRWASLADTYSDSGPASGIFDVPTQVDLPSEAGERINMLNRSADYSENSQNSRSRRVLPQVPLKEKIETPPPMIHIQPDPYLSTVEKSSQVQKHKWDPQKLNVQDDLDPDSLSDASKSDDGSVIEQCRKTPEVNQKTWSRPSKTPSNQDEAKDQDTPQKFSTATLTRQYGKPSKSNGAPPNIGQPSKNDSFGGDSLVSIVRQESYTKERASDDIQLSRLPQISSQPMSKDVFKGVNSQDTHSYLKDTEDVLASLEAKILEQQVNNGRSHPEDSFSGESDTSSTVSGKNPCVSVPKKPIVLKGFLKGTGQYTREPKTSEDQSGMKEKSDPGKRFQLRRNHSSQDFGNEQQSPATRQFPDTVSDQESSSLPYKKYTIPLQKEGTSKSKPTVTRSNSLSAPRPTRASMLRRGRLGEASDNEGTETDRTSQNSDVNPSANNRVAQTSKKLSRLDILALPRKRTGSFNTPSDTESSSASRTSFSNRSTESSSSVRKASVPDPRALLRKVTNAISRQPITRGRSSSAKYASSTASSRRRQKGSDYASTSEDEYESNQSTPKHKRSHHSGAQQSQPAGPVRAMPRSRDSEGEGQESDAFQNWTSHSAEIARLSQDLAKDLAILAQEIHDVAGDTETQSGSQEQTEPAISTQEELRHQIPESSLKVQKESPGAVEESEHTAEDENQANRRQDEAAVNSVLLNPVSQLSLAIRENTEQLTHKIKVLFHNRRDVLGEIEAKISAEDDSLSMKTSNKEVASILKELRRVQKQLEVINSIMDPQGNPELMRTFSSSAAAARGSRSALRDNRSAGGGAGATPRARRMFRSTDRDGSMV

Target ORF information:

RefSeq Version	XM_021467256.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio centrosomal protein 170B (cep170b), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021467256.1

ORF Insert Sequence:

ATGAGCGTGA CGTCGTGGTT TCTGGTGAGC AGCTCTGGCA CGCGCCACCG TTTGCCCCGA 
GAGATGATCT TTGTGGGTAG AGAAGACTGT GAACTCATGC TACAGTCGCG CAGTGTGGAC 
AAACAGCACG CTGTGATCAA CTATGATGCA TCTACAGATG AACATTTGGT CAAAGACTTG 
GGCAGCCTCA ACGGGACCTT CGTAAATGAC CTTCGCATCC CCGACCAGAC GTACATCATC 
CTGAAGTTGT CAGATGTGAT CCGCTTTGGT TATGATTCAC ACATATATAT CCTGGAGAGA 
AGCCAACACA AAGTGCCTGA GGAGGCTCTC AAGCATGAAA AATACACCAG TCAGCTTCAG 
CTGGAGACGC TGGAAAACAA GAAGCAGCTT CAGACAGGTG ACAGACGGAG AAACTCAGAC 
TCCACACGGG TCAAATCTGA AAAATCAGAG CGGAAAAACA TCACAGATGC ACCGATTTCC 
AGACCCACAC CTCTTTATGG TCAGCCGTCC TGGTGGGGTG AAGACGATGA AGATAAAGCT 
CAGCGCTGTG ATCCGAGCTC TCAGGAGCAA CATACAGAAA ACACGAAGGA AGGGACCAAA 
CATGATCTCA GCATTTCTCC AGACGATCTT CACGATGGCC AGAGCAAATC CAGCTTCATT 
CACAAAAAGG AGCCCAGTTA TTTTGAGATC CCTACTAAGG AGAACCATCC AAAGGCCAAA 
AGCACATTGA CTGAGATGCA GGAGATTCCC ACAAAAGACC CCGACCACAA GCCTCCGTCC 
GTCTCTCCGG TGGTGCAGGG TCACGCCTCC TTCACCATTG AGTTTGACGA GCAAACGCCT 
GGAAAGATGA AGATTAAGGA CCACATCTCT AAAATGTCCA TCCGCCAGCG CAGAAACCCC 
AGCAAGGAGT CTATAAGCCG CCTGACGGAG GTGATGTCTG TGGAGAATAA AGTGGCTGAT 
TGGTTGGTGC AGAGCGACAC GTCTTTGAGG AGTCTTGAGG AGCCCAGATC AGATCAGCTG 
CTCAACAAGA CCTTCAGAGA TGCACACCAT AATGAAGATC CAGATGTGGA AGAACAGGAG 
GAAATTCTGT CAGTCTCACA CTCAGCTGCA CAAAAAACCC CACCACAACA AGAGCCTGTA 
GATTATCCAT CCAATCAAAC GGCAGATTCA AAGAACCAAT GCAGAACGGA CCCCAAACAG 
GCTTACGTGA TTGAATTCTT TGATGATGCT CAACGCAAAA AGCGCTCGCA GTCTTTCACC 
AACAACATCT CCTCTCCAGA CTTCCAGTCT GCGCTGAAAA ACAAGTTGGA CAAACGAAAA 
GGCACAACTT TGTCATCCGG ACACAATCCA GCAACACAAC AGTTCACGAT TCCCCTCAAA 
GATTCAGATA GTCCACAGAG AGCTGAATCT CTGCGTCGCG AGACGAGCGA AATCCGAATG 
AGCACTTCTG AATTCAGCTC TCGCTCTGTG ACAAAACCGT TCGGCAGCAT CGGCCGCAGG 
TCCAAAATCT CGCAGGATTT CGCAGCGGAG TTGCTTCGGG TGTCAAAACA CAGTGCTGCG 
TCTTCATGGG ACTCCAATAC AACTTGGGTG AAAACGTCTT CGGCATTAAC GACAAATAAT 
GTGGTGCAGG AGAGTCTTTC TCAATCGCAG AATCACATGA TCAGATCTGA GCGAGTGGAG 
GATGGTGATA ATACTGAGGG CAAACCTCCC AAAGGGCAGG AGGAGGAGGA CAGTCTGAGT 
GATGCAGGAA CATACACTAT TGAAGCTGAA GGGCCGGATA GAGACGTTGC GGAGGCGCGG 
AGCTTGATTG ATCAGTTACA GTCAATGACG TCATTAACAC CTGGAGGCAC TCGATGGGTT 
TCTCGCTGGG CCAGTTTGGC TGATACCTAT TCAGACTCTG GTCCCGCTTC AGGAATATTT 
GATGTCCCAA CACAGGTTGA TCTTCCCTCC GAAGCTGGTG AAAGGATAAA CATGCTTAAC 
CGGAGCGCTG ACTACTCTGA AAATAGCCAA AACAGCAGAT CACGACGAGT TCTGCCTCAG 
GTTCCTTTAA AGGAGAAGAT TGAGACACCT CCACCCATGA TACACATCCA ACCTGATCCG 
TACCTGTCCA CTGTGGAGAA GAGTTCACAA GTTCAAAAGC ACAAGTGGGA TCCACAGAAA 
CTCAATGTGC AGGATGATCT GGATCCAGAC AGCCTTAGCG ATGCTAGCAA GTCTGATGAC 
GGATCTGTCA TTGAACAGTG CAGGAAGACT CCCGAAGTGA ACCAGAAGAC ATGGAGTCGG 
CCTTCAAAAA CACCATCCAA TCAAGACGAG GCAAAAGACC AGGATACACC TCAGAAGTTC 
TCCACTGCTA CGTTAACCCG ACAGTATGGG AAACCAAGCA AGAGCAATGG TGCACCACCC 
AATATTGGTC AGCCAAGCAA AAATGACTCT TTTGGAGGAG ACAGTTTAGT GTCTATTGTT 
AGACAAGAAA GTTACACCAA AGAAAGAGCG AGTGATGACA TCCAGTTGTC CAGATTGCCC 
CAGATATCGA GTCAGCCCAT GAGCAAAGAT GTTTTTAAGG GTGTAAACAG TCAAGATACT 
CATTCATACC TTAAAGACAC TGAGGATGTT CTTGCATCTC TGGAAGCCAA GATTCTGGAG 
CAACAAGTAA ACAACGGTCG CTCACATCCG GAGGACTCTT TCTCTGGAGA ATCCGACACT 
TCTAGTACTG TCAGTGGCAA AAACCCTTGC GTTTCTGTTC CCAAGAAGCC TATAGTACTC 
AAAGGATTCT TAAAAGGTAC CGGTCAGTAT ACCAGAGAAC CTAAAACCTC AGAAGATCAG 
TCTGGGATGA AGGAGAAGTC AGATCCAGGC AAGCGTTTTC AGCTCCGTCG AAATCATAGT 
TCTCAAGACT TTGGAAATGA ACAGCAAAGT CCTGCTACAC GGCAGTTTCC TGACACTGTC 
TCAGACCAGG AATCAAGTTC TTTACCTTAT AAAAAGTACA CTATTCCTCT TCAGAAGGAA 
GGTACGAGCA AATCCAAACC TACCGTAACT CGCTCCAATA GTCTTTCCGC TCCAAGACCA 
ACAAGAGCGT CAATGCTTCG ACGTGGACGT TTGGGTGAAG CCTCTGATAA TGAAGGTACC 
GAAACCGATC GAACGTCTCA GAATTCCGAC GTCAATCCTT CTGCAAACAA TCGAGTGGCT 
CAAACAAGCA AGAAACTTTC TCGATTGGAC ATTTTGGCTC TGCCTAGGAA AAGGACGGGA 
TCTTTTAACA CCCCGAGTGA CACAGAGTCT TCTTCTGCGA GCCGGACGAG CTTCTCGAAC 
CGCAGCACAG AGTCGAGCAG CTCCGTGCGG AAAGCATCAG TTCCGGATCC CAGGGCTCTT 
CTGCGTAAGG TTACAAATGC CATCAGCAGG CAGCCGATCA CTCGCGGACG CTCGAGCAGT 
GCCAAATACG CCAGCAGCAC CGCCAGCTCC AGGAGGCGTC AGAAGGGTTC AGACTACGCC 
TCCACCTCGG AGGACGAGTA CGAGTCGAAC CAAAGCACTC CTAAACACAA ACGCTCCCAT 
CATTCAGGAG CGCAGCAGAG CCAGCCGGCA GGTCCGGTGA GAGCCATGCC CAGATCCAGA 
GACTCTGAGG GGGAAGGACA GGAGAGCGAC GCCTTCCAGA ACTGGACGTC TCACAGTGCC 
GAGATCGCCA GATTGAGTCA GGATCTCGCT AAAGATTTGG CTATTTTGGC CCAAGAGATT 
CATGATGTCG CAGGAGACAC TGAGACTCAG AGCGGATCAC AAGAGCAGAC GGAGCCCGCC 
ATATCCACAC AAGAGGAGTT GCGGCATCAG ATTCCTGAAT CCAGTCTGAA AGTGCAGAAA 
GAGTCTCCTG GAGCAGTCGA AGAGTCTGAA CACACCGCTG AAGATGAGAA TCAAGCCAAT 
AGGAGACAAG ACGAGGCTGC TGTGAACAGT GTGCTCCTCA ATCCTGTGTC TCAGCTTTCA 
CTCGCCATCA GGGAAAACAC CGAACAGCTC ACACACAAAA TCAAGGTTTT GTTTCATAAT 
CGGAGAGATG TTTTGGGTGA AATCGAGGCA AAGATCAGTG CAGAGGATGA TTCTCTATCT 
ATGAAAACCT CAAACAAGGA AGTGGCGTCG ATCCTGAAAG AGCTGAGGAG AGTCCAGAAG 
CAGCTGGAAG TGATCAACAG CATCATGGAT CCGCAGGGGA ACCCGGAGCT GATGCGGACG 
TTCTCCTCAT CTGCAGCTGC AGCGCGTGGA TCCAGATCTG CTCTGCGGGA CAACAGATCT 
GCAGGAGGAG GAGCAGGAGC AACACCCAGA GCCAGACGCA TGTTCAGGAG CACAGACAGA 
GACGGCTCGA TGGTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cep170b ( XM_017351731.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017207220.2 Danio rerio centrosomal protein 170B (cep170b), transcript variant X1, mRNA.

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CloneID	ODa149603
Clone ID Related Accession (Same CDS sequence)	XM_017351731.2
Accession Version	XM_017351731.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4509bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	centrosomal protein of 170 kDa protein B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017351731.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGTGA CGTCGTGGTT TCTGGTGAGC AGCTCTGGCA CGCGCCACCG TTTGCCCCGA 
GAGATGATCT TTGTGGGTAG AGAAGACTGT GAACTCATGC TACAGTCGCG CAGTGTGGAC 
AAACAGCACG CTGTGATCAA CTATGATGCA TCTACAGATG AACATTTGGT CAAAGACTTG 
GGCAGCCTCA ACGGGACCTT CGTAAATGAC CTTCGCATCC CCGACCAGAC GTACATCATC 
CTGAAGTTGT CAGATGTGAT CCGCTTTGGT TATGATTCAC ACATATATAT CCTGGAGAGA 
AGCCAACACA AAGTGCCTGA GGAGGCTCTC AAGCATGAAA AATACACCAG TCAGCTTCAG 
CTGGAGACGC TGGAAAACAA GAAGCAGCTT CAGACAGGTG ACAGACGGAG AAACTCAGAC 
TCCACACGGG TCAAATCTGA AAAATCAGAG CGGAAAAACA TCACAGATGC ACCGATTTCC 
AGACCCACAC CTCTTTATGG TCAGCCGTCC TGGTGGGGTG AAGACGATGA AGATAAAGCT 
CAGCGCTGTG ATCCGAGCTC TCAGGAGCAA CATACAGAAA ACACGAAGGA AGGGACCAAA 
CATGATCTCA GCATTTCTCC AGACGATCTT CACGATGGCC AGAGCAAATC CAGCTTCATT 
CACAAAAAGG AGCCCAGTTA TTTTGAGATC CCTACTAAGG AGAACCATCC AAAGGCCAAA 
AGCACATTGA CTGAGATGCA GGAGATTCCC ACAAAAGACC CCGACCACAA GCCTCCGTCC 
GTCTCTCCGG TGGTGCAGGG TCACGCCTCC TTCACCATTG AGTTTGACGA GCAAACGCCT 
GGAAAGATGA AGATTAAGGA CCACATCTCT AAAATGTCCA TCCGCCAGCG CAGAAACCCC 
AGCAAGGAGT CTATAAGCCG CCTGACGGAG GTGATGTCTG TGGAGAATAA AGTGGCTGAT 
TGGTTGGTGC AGAGCGACAC GTCTTTGAGG AGTCTTGAGG AGCCCAGATC AGATCAGCTG 
CTCAACAAGA CCTTCAGAGA TGCACACCAT AATGAAGATC CAGATGTGGA AGAACAGGAG 
GAAATTCTGT CAGTCTCACA CTCAGCTGCA CAAAAAACCC CACCACAACA AGAGCCTGTA 
GATTATCCAT CCAATCAAAC GGCAGATTCA AAGAACCAAT GCAGAACGGA CCCCAAACAG 
GCTTACGTGA TTGAATTCTT TGATGATGCT CAACGCAAAA AGCGCTCGCA GTCTTTCACC 
AACAACATCT CCTCTCCAGA CTTCCAGTCT GCGCTGAAAA ACAAGTTGGA CAAACGAAAA 
GGCACAACTT TGTCATCCGG ACACAATCCA GCAACACAAC AGTTCACGAT TCCCCTCAAA 
GATTCAGATA GTCCACAGAG AGCTGAATCT CTGCGTCGCG AGACGAGCGA AATCCGAATG 
AGCACTTCTG AATTCAGCTC TCGCTCTGTG ACAAAACCGT TCGGCAGCAT CGGCCGCAGG 
TCCAAAATCT CGCAGGATTT CGCAGCGGAG TTGCTTCGGG TGTCAAAACA CAGTGCTGCG 
TCTTCATGGG ACTCCAATAC AACTTGGGTG AAAACGTCTT CGGCATTAAC GACAAATAAT 
GTGGTGCAGG AGAGTCTTTC TCAATCGCAG AATCACATGA TCAGATCTGA GCGAGTGGAG 
GATGGTGATA ATACTGAGGG CAAACCTCCC AAAGGGCAGG AGGAGGAGGA CAGTCTGAGT 
GATGCAGGAA CATACACTAT TGAAGCTGAA GGGCCGGATA GAGACGTTGC GGAGGCGCGG 
AGCTTGATTG ATCAGGTGTT TGGTGTTGGA GAAAACAGCC AAACCACAGC AGCATCAGCA 
GCAGATAAGG CTGTAGTTGG TGACTCTGAG GCTCATGATA GCCTTCTTGT CAATGAGAAT 
AGTTCAGGCC TGATAATGGG TCAGTTCCCA TCAGAACATA GTGCCACAGC CAAGAGTCCT 
GCGCAGTTAC AGTCAATGAC GTCATTAACA CCTGGAGGCA CTCGATGGGT TTCTCGCTGG 
GCCAGTTTGG CTGATACCTA TTCAGACTCT GGTCCCGCTT CAGGAATATT TGATGTCCCA 
ACACAGGTTG ATCTTCCCTC CGAAGCTGGT GAAAGGATAA ACATGCTTAA CCGGAGCGCT 
GACTACTCTG AAAATAGCCA AAACAGCAGA TCACGACGAG TTCTGCCTCA GGTTCCTTTA 
AAGGAGAAGA TTGAGACACC TCCACCCATG ATACACATCC AACCTGATCC GTACCTGTCC 
ACTGTGGAGA AGAGTTCACA AGTTCAAAAG CACAAGTGGG ATCCACAGAA ACTCAATGTG 
CAGGATGATC TGGATCCAGA CAGCCTTAGC GATGCTAGCA AGTCTGATGA CGGATCTGTC 
ATTGAACAGT GCAGGAAGAC TCCCGAAGTG AACCAGAAGA CATGGAGTCG GCCTTCAAAA 
ACACCATCCA ATCAAGACGA GGCAAAAGAC CAGGATACAC CTCAGAAGTT CTCCACTGCT 
ACGTTAACCC GACAGTATGG GAAACCAAGC AAGAGCAATG GTGCACCACC CAATATTGGT 
CAGCCAAGCA AAAATGACTC TTTTGGAGGA GACAGTTTAG TGTCTATTGT TAGACAAGAA 
AGTTACACCA AAGAAAGAGC GAGTGATGAC ATCCAGTTGT CCAGATTGCC CCAGATATCG 
AGTCAGCCCA TGAGCAAAGA TGTTTTTAAG GGTGTAAACA GTCAAGATAC TCATTCATAC 
CTTAAAGACA CTGAGGATGT TCTTGCATCT CTGGAAGCCA AGATTCTGGA GCAACAAGTA 
AACAACGGTC GCTCACATCC GGAGGACTCT TTCTCTGGAG AATCCGACAC TTCTAGTACT 
GTCAGTGGCA AAAACCCTTG CGTTTCTGTT CCCAAGAAGC CTATAGTACT CAAAGGATTC 
TTAAAAGGTA CCGGTCAGTA TACCAGAGAA CCTAAAACCT CAGAAGATCA GTCTGGGATG 
AAGGAGAAGT CAGATCCAGG CAAGCGTTTT CAGCTCCGTC GAAATCATAG TTCTCAAGAC 
TTTGGAAATG AACAGCAAAG TCCTGCTACA CGGCAGTTTC CTGACACTGT CTCAGACCAG 
GAATCAAGTT CTTTACCTTA TAAAAAGTAC ACTATTCCTC TTCAGAAGGA AGGTACGAGC 
AAATCCAAAC CTACCGTAAC TCGCTCCAAT AGTCTTTCCG CTCCAAGACC AACAAGAGCG 
TCAATGCTTC GACGTGGACG TTTGGGTGAA GCCTCTGATA ATGAAGGTAC CGAAACCGAT 
CGAACGTCTC AGAATTCCGA CGTCAATCCT TCTGCAAACA ATCGAGTGGC TCAAACAAGC 
AAGAAACTTT CTCGATTGGA CATTTTGGCT CTGCCTAGGA AAAGGACGGG ATCTTTTAAC 
ACCCCGAGTG ACACAGAGTC TTCTTCTGCG AGCCGGACGA GCTTCTCGAA CCGCAGCACA 
GAGTCGAGCA GCTCCGTGCG GAAAGCATCA GTTCCGGATC CCAGGGCTCT TCTGCGTAAG 
GTTACAAATG CCATCAGCAG GCAGCCGATC ACTCGCGGAC GCTCGAGCAG TGCCAAATAC 
GCCAGCAGCA CCGCCAGCTC CAGGAGGCGT CAGAAGGGTT CAGACTACGC CTCCACCTCG 
GAGGACGAGT ACGAGTCGAA CCAAAGCACT CCTAAACACA AACGCTCCCA TCATTCAGGA 
GCGCAGCAGA GCCAGCCGGC AGGTCCGGTG AGAGCCATGC CCAGATCCAG AGACTCTGAG 
GGGGAAGGAC AGGAGAGCGA CGCCTTCCAG AACTGGACGT CTCACAGTGC CGAGATCGCC 
AGATTGAGTC AGGATCTCGC TAAAGATTTG GCTATTTTGG CCCAAGAGAT TCATGATGTC 
GCAGGAGACA CTGAGACTCA GAGCGGATCA CAAGAGCAGA CGGAGCCCGC CATATCCACA 
CAAGAGGAGT TGCGGCATCA GATTCCTGAA TCCAGTCTGA AAGTGCAGAA AGAGTCTCCT 
GGAGCAGTCG AAGAGTCTGA ACACACCGCT GAAGATGAGA ATCAAGCCAA TAGGAGACAA 
GACGAGGCTG CTGTGAACAG TGTGCTCCTC AATCCTGTGT CTCAGCTTTC ACTCGCCATC 
AGGGAAAACA CCGAACAGCT CACACACAAA ATCAAGGTTT TGTTTCATAA TCGGAGAGAT 
GTTTTGGGTG AAATCGAGGC AAAGATCAGT GCAGAGGATG ATTCTCTATC TATGAAAACC 
TCAAACAAGG AAGTGGCGTC GATCCTGAAA GAGCTGAGGA GAGTCCAGAA GCAGCTGGAA 
GTGATCAACA GCATCATGGA TCCGCAGGGG AACCCGGAGC TGATGCGGAC GTTCTCCTCA 
TCTGCAGCTG CAGCGCGTGG ATCCAGATCT GCTCTGCGGG ACAACAGATC TGCAGGAGGA 
GGAGCAGGAG CAACACCCAG AGCCAGACGC ATGTTCAGGA GCACAGACAG AGACGGCTCG 
ATGGTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017207220.2
CDS	294..4802
Translation	MSVTSWFLVSSSGTRHRLPREMIFVGREDCELMLQSRSVDKQHAVINYDASTDEHLVKDLGSLNGTFVNDLRIPDQTYIILKLSDVIRFGYDSHIYILERSQHKVPEEALKHEKYTSQLQLETLENKKQLQTGDRRRNSDSTRVKSEKSERKNITDAPISRPTPLYGQPSWWGEDDEDKAQRCDPSSQEQHTENTKEGTKHDLSISPDDLHDGQSKSSFIHKKEPSYFEIPTKENHPKAKSTLTEMQEIPTKDPDHKPPSVSPVVQGHASFTIEFDEQTPGKMKIKDHISKMSIRQRRNPSKESISRLTEVMSVENKVADWLVQSDTSLRSLEEPRSDQLLNKTFRDAHHNEDPDVEEQEEILSVSHSAAQKTPPQQEPVDYPSNQTADSKNQCRTDPKQAYVIEFFDDAQRKKRSQSFTNNISSPDFQSALKNKLDKRKGTTLSSGHNPATQQFTIPLKDSDSPQRAESLRRETSEIRMSTSEFSSRSVTKPFGSIGRRSKISQDFAAELLRVSKHSAASSWDSNTTWVKTSSALTTNNVVQESLSQSQNHMIRSERVEDGDNTEGKPPKGQEEEDSLSDAGTYTIEAEGPDRDVAEARSLIDQVFGVGENSQTTAASAADKAVVGDSEAHDSLLVNENSSGLIMGQFPSEHSATAKSPAQLQSMTSLTPGGTRWVSRWASLADTYSDSGPASGIFDVPTQVDLPSEAGERINMLNRSADYSENSQNSRSRRVLPQVPLKEKIETPPPMIHIQPDPYLSTVEKSSQVQKHKWDPQKLNVQDDLDPDSLSDASKSDDGSVIEQCRKTPEVNQKTWSRPSKTPSNQDEAKDQDTPQKFSTATLTRQYGKPSKSNGAPPNIGQPSKNDSFGGDSLVSIVRQESYTKERASDDIQLSRLPQISSQPMSKDVFKGVNSQDTHSYLKDTEDVLASLEAKILEQQVNNGRSHPEDSFSGESDTSSTVSGKNPCVSVPKKPIVLKGFLKGTGQYTREPKTSEDQSGMKEKSDPGKRFQLRRNHSSQDFGNEQQSPATRQFPDTVSDQESSSLPYKKYTIPLQKEGTSKSKPTVTRSNSLSAPRPTRASMLRRGRLGEASDNEGTETDRTSQNSDVNPSANNRVAQTSKKLSRLDILALPRKRTGSFNTPSDTESSSASRTSFSNRSTESSSSVRKASVPDPRALLRKVTNAISRQPITRGRSSSAKYASSTASSRRRQKGSDYASTSEDEYESNQSTPKHKRSHHSGAQQSQPAGPVRAMPRSRDSEGEGQESDAFQNWTSHSAEIARLSQDLAKDLAILAQEIHDVAGDTETQSGSQEQTEPAISTQEELRHQIPESSLKVQKESPGAVEESEHTAEDENQANRRQDEAAVNSVLLNPVSQLSLAIRENTEQLTHKIKVLFHNRRDVLGEIEAKISAEDDSLSMKTSNKEVASILKELRRVQKQLEVINSIMDPQGNPELMRTFSSSAAAARGSRSALRDNRSAGGGAGATPRARRMFRSTDRDGSMV

Target ORF information:

RefSeq Version	XM_017351731.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio centrosomal protein 170B (cep170b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017351731.2

ORF Insert Sequence:

ATGAGCGTGA CGTCGTGGTT TCTGGTGAGC AGCTCTGGCA CGCGCCACCG TTTGCCCCGA 
GAGATGATCT TTGTGGGTAG AGAAGACTGT GAACTCATGC TACAGTCGCG CAGTGTGGAC 
AAACAGCACG CTGTGATCAA CTATGATGCA TCTACAGATG AACATTTGGT CAAAGACTTG 
GGCAGCCTCA ACGGGACCTT CGTAAATGAC CTTCGCATCC CCGACCAGAC GTACATCATC 
CTGAAGTTGT CAGATGTGAT CCGCTTTGGT TATGATTCAC ACATATATAT CCTGGAGAGA 
AGCCAACACA AAGTGCCTGA GGAGGCTCTC AAGCATGAAA AATACACCAG TCAGCTTCAG 
CTGGAGACGC TGGAAAACAA GAAGCAGCTT CAGACAGGTG ACAGACGGAG AAACTCAGAC 
TCCACACGGG TCAAATCTGA AAAATCAGAG CGGAAAAACA TCACAGATGC ACCGATTTCC 
AGACCCACAC CTCTTTATGG TCAGCCGTCC TGGTGGGGTG AAGACGATGA AGATAAAGCT 
CAGCGCTGTG ATCCGAGCTC TCAGGAGCAA CATACAGAAA ACACGAAGGA AGGGACCAAA 
CATGATCTCA GCATTTCTCC AGACGATCTT CACGATGGCC AGAGCAAATC CAGCTTCATT 
CACAAAAAGG AGCCCAGTTA TTTTGAGATC CCTACTAAGG AGAACCATCC AAAGGCCAAA 
AGCACATTGA CTGAGATGCA GGAGATTCCC ACAAAAGACC CCGACCACAA GCCTCCGTCC 
GTCTCTCCGG TGGTGCAGGG TCACGCCTCC TTCACCATTG AGTTTGACGA GCAAACGCCT 
GGAAAGATGA AGATTAAGGA CCACATCTCT AAAATGTCCA TCCGCCAGCG CAGAAACCCC 
AGCAAGGAGT CTATAAGCCG CCTGACGGAG GTGATGTCTG TGGAGAATAA AGTGGCTGAT 
TGGTTGGTGC AGAGCGACAC GTCTTTGAGG AGTCTTGAGG AGCCCAGATC AGATCAGCTG 
CTCAACAAGA CCTTCAGAGA TGCACACCAT AATGAAGATC CAGATGTGGA AGAACAGGAG 
GAAATTCTGT CAGTCTCACA CTCAGCTGCA CAAAAAACCC CACCACAACA AGAGCCTGTA 
GATTATCCAT CCAATCAAAC GGCAGATTCA AAGAACCAAT GCAGAACGGA CCCCAAACAG 
GCTTACGTGA TTGAATTCTT TGATGATGCT CAACGCAAAA AGCGCTCGCA GTCTTTCACC 
AACAACATCT CCTCTCCAGA CTTCCAGTCT GCGCTGAAAA ACAAGTTGGA CAAACGAAAA 
GGCACAACTT TGTCATCCGG ACACAATCCA GCAACACAAC AGTTCACGAT TCCCCTCAAA 
GATTCAGATA GTCCACAGAG AGCTGAATCT CTGCGTCGCG AGACGAGCGA AATCCGAATG 
AGCACTTCTG AATTCAGCTC TCGCTCTGTG ACAAAACCGT TCGGCAGCAT CGGCCGCAGG 
TCCAAAATCT CGCAGGATTT CGCAGCGGAG TTGCTTCGGG TGTCAAAACA CAGTGCTGCG 
TCTTCATGGG ACTCCAATAC AACTTGGGTG AAAACGTCTT CGGCATTAAC GACAAATAAT 
GTGGTGCAGG AGAGTCTTTC TCAATCGCAG AATCACATGA TCAGATCTGA GCGAGTGGAG 
GATGGTGATA ATACTGAGGG CAAACCTCCC AAAGGGCAGG AGGAGGAGGA CAGTCTGAGT 
GATGCAGGAA CATACACTAT TGAAGCTGAA GGGCCGGATA GAGACGTTGC GGAGGCGCGG 
AGCTTGATTG ATCAGGTGTT TGGTGTTGGA GAAAACAGCC AAACCACAGC AGCATCAGCA 
GCAGATAAGG CTGTAGTTGG TGACTCTGAG GCTCATGATA GCCTTCTTGT CAATGAGAAT 
AGTTCAGGCC TGATAATGGG TCAGTTCCCA TCAGAACATA GTGCCACAGC CAAGAGTCCT 
GCGCAGTTAC AGTCAATGAC GTCATTAACA CCTGGAGGCA CTCGATGGGT TTCTCGCTGG 
GCCAGTTTGG CTGATACCTA TTCAGACTCT GGTCCCGCTT CAGGAATATT TGATGTCCCA 
ACACAGGTTG ATCTTCCCTC CGAAGCTGGT GAAAGGATAA ACATGCTTAA CCGGAGCGCT 
GACTACTCTG AAAATAGCCA AAACAGCAGA TCACGACGAG TTCTGCCTCA GGTTCCTTTA 
AAGGAGAAGA TTGAGACACC TCCACCCATG ATACACATCC AACCTGATCC GTACCTGTCC 
ACTGTGGAGA AGAGTTCACA AGTTCAAAAG CACAAGTGGG ATCCACAGAA ACTCAATGTG 
CAGGATGATC TGGATCCAGA CAGCCTTAGC GATGCTAGCA AGTCTGATGA CGGATCTGTC 
ATTGAACAGT GCAGGAAGAC TCCCGAAGTG AACCAGAAGA CATGGAGTCG GCCTTCAAAA 
ACACCATCCA ATCAAGACGA GGCAAAAGAC CAGGATACAC CTCAGAAGTT CTCCACTGCT 
ACGTTAACCC GACAGTATGG GAAACCAAGC AAGAGCAATG GTGCACCACC CAATATTGGT 
CAGCCAAGCA AAAATGACTC TTTTGGAGGA GACAGTTTAG TGTCTATTGT TAGACAAGAA 
AGTTACACCA AAGAAAGAGC GAGTGATGAC ATCCAGTTGT CCAGATTGCC CCAGATATCG 
AGTCAGCCCA TGAGCAAAGA TGTTTTTAAG GGTGTAAACA GTCAAGATAC TCATTCATAC 
CTTAAAGACA CTGAGGATGT TCTTGCATCT CTGGAAGCCA AGATTCTGGA GCAACAAGTA 
AACAACGGTC GCTCACATCC GGAGGACTCT TTCTCTGGAG AATCCGACAC TTCTAGTACT 
GTCAGTGGCA AAAACCCTTG CGTTTCTGTT CCCAAGAAGC CTATAGTACT CAAAGGATTC 
TTAAAAGGTA CCGGTCAGTA TACCAGAGAA CCTAAAACCT CAGAAGATCA GTCTGGGATG 
AAGGAGAAGT CAGATCCAGG CAAGCGTTTT CAGCTCCGTC GAAATCATAG TTCTCAAGAC 
TTTGGAAATG AACAGCAAAG TCCTGCTACA CGGCAGTTTC CTGACACTGT CTCAGACCAG 
GAATCAAGTT CTTTACCTTA TAAAAAGTAC ACTATTCCTC TTCAGAAGGA AGGTACGAGC 
AAATCCAAAC CTACCGTAAC TCGCTCCAAT AGTCTTTCCG CTCCAAGACC AACAAGAGCG 
TCAATGCTTC GACGTGGACG TTTGGGTGAA GCCTCTGATA ATGAAGGTAC CGAAACCGAT 
CGAACGTCTC AGAATTCCGA CGTCAATCCT TCTGCAAACA ATCGAGTGGC TCAAACAAGC 
AAGAAACTTT CTCGATTGGA CATTTTGGCT CTGCCTAGGA AAAGGACGGG ATCTTTTAAC 
ACCCCGAGTG ACACAGAGTC TTCTTCTGCG AGCCGGACGA GCTTCTCGAA CCGCAGCACA 
GAGTCGAGCA GCTCCGTGCG GAAAGCATCA GTTCCGGATC CCAGGGCTCT TCTGCGTAAG 
GTTACAAATG CCATCAGCAG GCAGCCGATC ACTCGCGGAC GCTCGAGCAG TGCCAAATAC 
GCCAGCAGCA CCGCCAGCTC CAGGAGGCGT CAGAAGGGTT CAGACTACGC CTCCACCTCG 
GAGGACGAGT ACGAGTCGAA CCAAAGCACT CCTAAACACA AACGCTCCCA TCATTCAGGA 
GCGCAGCAGA GCCAGCCGGC AGGTCCGGTG AGAGCCATGC CCAGATCCAG AGACTCTGAG 
GGGGAAGGAC AGGAGAGCGA CGCCTTCCAG AACTGGACGT CTCACAGTGC CGAGATCGCC 
AGATTGAGTC AGGATCTCGC TAAAGATTTG GCTATTTTGG CCCAAGAGAT TCATGATGTC 
GCAGGAGACA CTGAGACTCA GAGCGGATCA CAAGAGCAGA CGGAGCCCGC CATATCCACA 
CAAGAGGAGT TGCGGCATCA GATTCCTGAA TCCAGTCTGA AAGTGCAGAA AGAGTCTCCT 
GGAGCAGTCG AAGAGTCTGA ACACACCGCT GAAGATGAGA ATCAAGCCAA TAGGAGACAA 
GACGAGGCTG CTGTGAACAG TGTGCTCCTC AATCCTGTGT CTCAGCTTTC ACTCGCCATC 
AGGGAAAACA CCGAACAGCT CACACACAAA ATCAAGGTTT TGTTTCATAA TCGGAGAGAT 
GTTTTGGGTG AAATCGAGGC AAAGATCAGT GCAGAGGATG ATTCTCTATC TATGAAAACC 
TCAAACAAGG AAGTGGCGTC GATCCTGAAA GAGCTGAGGA GAGTCCAGAA GCAGCTGGAA 
GTGATCAACA GCATCATGGA TCCGCAGGGG AACCCGGAGC TGATGCGGAC GTTCTCCTCA 
TCTGCAGCTG CAGCGCGTGG ATCCAGATCT GCTCTGCGGG ACAACAGATC TGCAGGAGGA 
GGAGCAGGAG CAACACCCAG AGCCAGACGC ATGTTCAGGA GCACAGACAG AGACGGCTCG 
ATGGTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cep170b ( XM_021467254.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021322929.1 Danio rerio centrosomal protein 170B (cep170b), transcript variant X2, mRNA.

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CloneID	ODa149604
Clone ID Related Accession (Same CDS sequence)	XM_021467254.1
Accession Version	XM_021467254.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4503bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	centrosomal protein of 170 kDa protein B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGTGA CGTCGTGGTT TCTGGTGAGC AGCTCTGGCA CGCGCCACCG TTTGCCCCGA 
GAGATGATCT TTGTGGGTAG AGAAGACTGT GAACTCATGC TACAGTCGCG CAGTGTGGAC 
AAACAGCACG CTGTGATCAA CTATGATGCA TCTACAGATG AACATTTGGT CAAAGACTTG 
GGCAGCCTCA ACGGGACCTT CGTAAATGAC CTTCGCATCC CCGACCAGAC GTACATCATC 
CTGAAGTTGT CAGATGTGAT CCGCTTTGGT TATGATTCAC ACATATATAT CCTGGAGAGA 
AGCCAACACA AAGTGCCTGA GGAGGCTCTC AAGCATGAAA AATACACCAG TCAGCTTCAG 
CTGGAGACGC TGGAAAACAA GAAGCAGCTT CAGACAGGTG ACAGACGGAG AAACTCAGAC 
TCCACACGGG TCAAATCTGA AAAATCAGAG CGGAAAAACA TCACAGATGC ACCGATTTCC 
AGACCCACAC CTCTTTATGG TCAGCCGTCC TGGTGGGGTG AAGACGATGA AGATAAAGCT 
CAGCGCTGTG ATCCGAGCTC TCAGGAGCAA CATACAGAAA ACACGAAGGA AGGGACCAAA 
CATGATCTCA GCATTTCTCC AGACGATCTT CACGATGGCC AGAGCAAATC CAGCTTCATT 
CACAAAAAGG AGCCCAGTTA TTTTGAGATC CCTACTAAGG AGAACCATCC AAAGGCCAAA 
AGCACATTGA CTGAGGAGAT TCCCACAAAA GACCCCGACC ACAAGCCTCC GTCCGTCTCT 
CCGGTGGTGC AGGGTCACGC CTCCTTCACC ATTGAGTTTG ACGAGCAAAC GCCTGGAAAG 
ATGAAGATTA AGGACCACAT CTCTAAAATG TCCATCCGCC AGCGCAGAAA CCCCAGCAAG 
GAGTCTATAA GCCGCCTGAC GGAGGTGATG TCTGTGGAGA ATAAAGTGGC TGATTGGTTG 
GTGCAGAGCG ACACGTCTTT GAGGAGTCTT GAGGAGCCCA GATCAGATCA GCTGCTCAAC 
AAGACCTTCA GAGATGCACA CCATAATGAA GATCCAGATG TGGAAGAACA GGAGGAAATT 
CTGTCAGTCT CACACTCAGC TGCACAAAAA ACCCCACCAC AACAAGAGCC TGTAGATTAT 
CCATCCAATC AAACGGCAGA TTCAAAGAAC CAATGCAGAA CGGACCCCAA ACAGGCTTAC 
GTGATTGAAT TCTTTGATGA TGCTCAACGC AAAAAGCGCT CGCAGTCTTT CACCAACAAC 
ATCTCCTCTC CAGACTTCCA GTCTGCGCTG AAAAACAAGT TGGACAAACG AAAAGGCACA 
ACTTTGTCAT CCGGACACAA TCCAGCAACA CAACAGTTCA CGATTCCCCT CAAAGATTCA 
GATAGTCCAC AGAGAGCTGA ATCTCTGCGT CGCGAGACGA GCGAAATCCG AATGAGCACT 
TCTGAATTCA GCTCTCGCTC TGTGACAAAA CCGTTCGGCA GCATCGGCCG CAGGTCCAAA 
ATCTCGCAGG ATTTCGCAGC GGAGTTGCTT CGGGTGTCAA AACACAGTGC TGCGTCTTCA 
TGGGACTCCA ATACAACTTG GGTGAAAACG TCTTCGGCAT TAACGACAAA TAATGTGGTG 
CAGGAGAGTC TTTCTCAATC GCAGAATCAC ATGATCAGAT CTGAGCGAGT GGAGGATGGT 
GATAATACTG AGGGCAAACC TCCCAAAGGG CAGGAGGAGG AGGACAGTCT GAGTGATGCA 
GGAACATACA CTATTGAAGC TGAAGGGCCG GATAGAGACG TTGCGGAGGC GCGGAGCTTG 
ATTGATCAGG TGTTTGGTGT TGGAGAAAAC AGCCAAACCA CAGCAGCATC AGCAGCAGAT 
AAGGCTGTAG TTGGTGACTC TGAGGCTCAT GATAGCCTTC TTGTCAATGA GAATAGTTCA 
GGCCTGATAA TGGGTCAGTT CCCATCAGAA CATAGTGCCA CAGCCAAGAG TCCTGCGCAG 
TTACAGTCAA TGACGTCATT AACACCTGGA GGCACTCGAT GGGTTTCTCG CTGGGCCAGT 
TTGGCTGATA CCTATTCAGA CTCTGGTCCC GCTTCAGGAA TATTTGATGT CCCAACACAG 
GTTGATCTTC CCTCCGAAGC TGGTGAAAGG ATAAACATGC TTAACCGGAG CGCTGACTAC 
TCTGAAAATA GCCAAAACAG CAGATCACGA CGAGTTCTGC CTCAGGTTCC TTTAAAGGAG 
AAGATTGAGA CACCTCCACC CATGATACAC ATCCAACCTG ATCCGTACCT GTCCACTGTG 
GAGAAGAGTT CACAAGTTCA AAAGCACAAG TGGGATCCAC AGAAACTCAA TGTGCAGGAT 
GATCTGGATC CAGACAGCCT TAGCGATGCT AGCAAGTCTG ATGACGGATC TGTCATTGAA 
CAGTGCAGGA AGACTCCCGA AGTGAACCAG AAGACATGGA GTCGGCCTTC AAAAACACCA 
TCCAATCAAG ACGAGGCAAA AGACCAGGAT ACACCTCAGA AGTTCTCCAC TGCTACGTTA 
ACCCGACAGT ATGGGAAACC AAGCAAGAGC AATGGTGCAC CACCCAATAT TGGTCAGCCA 
AGCAAAAATG ACTCTTTTGG AGGAGACAGT TTAGTGTCTA TTGTTAGACA AGAAAGTTAC 
ACCAAAGAAA GAGCGAGTGA TGACATCCAG TTGTCCAGAT TGCCCCAGAT ATCGAGTCAG 
CCCATGAGCA AAGATGTTTT TAAGGGTGTA AACAGTCAAG ATACTCATTC ATACCTTAAA 
GACACTGAGG ATGTTCTTGC ATCTCTGGAA GCCAAGATTC TGGAGCAACA AGTAAACAAC 
GGTCGCTCAC ATCCGGAGGA CTCTTTCTCT GGAGAATCCG ACACTTCTAG TACTGTCAGT 
GGCAAAAACC CTTGCGTTTC TGTTCCCAAG AAGCCTATAG TACTCAAAGG ATTCTTAAAA 
GGTACCGGTC AGTATACCAG AGAACCTAAA ACCTCAGAAG ATCAGTCTGG GATGAAGGAG 
AAGTCAGATC CAGGCAAGCG TTTTCAGCTC CGTCGAAATC ATAGTTCTCA AGACTTTGGA 
AATGAACAGC AAAGTCCTGC TACACGGCAG TTTCCTGACA CTGTCTCAGA CCAGGAATCA 
AGTTCTTTAC CTTATAAAAA GTACACTATT CCTCTTCAGA AGGAAGGTAC GAGCAAATCC 
AAACCTACCG TAACTCGCTC CAATAGTCTT TCCGCTCCAA GACCAACAAG AGCGTCAATG 
CTTCGACGTG GACGTTTGGG TGAAGCCTCT GATAATGAAG GTACCGAAAC CGATCGAACG 
TCTCAGAATT CCGACGTCAA TCCTTCTGCA AACAATCGAG TGGCTCAAAC AAGCAAGAAA 
CTTTCTCGAT TGGACATTTT GGCTCTGCCT AGGAAAAGGA CGGGATCTTT TAACACCCCG 
AGTGACACAG AGTCTTCTTC TGCGAGCCGG ACGAGCTTCT CGAACCGCAG CACAGAGTCG 
AGCAGCTCCG TGCGGAAAGC ATCAGTTCCG GATCCCAGGG CTCTTCTGCG TAAGGTTACA 
AATGCCATCA GCAGGCAGCC GATCACTCGC GGACGCTCGA GCAGTGCCAA ATACGCCAGC 
AGCACCGCCA GCTCCAGGAG GCGTCAGAAG GGTTCAGACT ACGCCTCCAC CTCGGAGGAC 
GAGTACGAGT CGAACCAAAG CACTCCTAAA CACAAACGCT CCCATCATTC AGGAGCGCAG 
CAGAGCCAGC CGGCAGGTCC GGTGAGAGCC ATGCCCAGAT CCAGAGACTC TGAGGGGGAA 
GGACAGGAGA GCGACGCCTT CCAGAACTGG ACGTCTCACA GTGCCGAGAT CGCCAGATTG 
AGTCAGGATC TCGCTAAAGA TTTGGCTATT TTGGCCCAAG AGATTCATGA TGTCGCAGGA 
GACACTGAGA CTCAGAGCGG ATCACAAGAG CAGACGGAGC CCGCCATATC CACACAAGAG 
GAGTTGCGGC ATCAGATTCC TGAATCCAGT CTGAAAGTGC AGAAAGAGTC TCCTGGAGCA 
GTCGAAGAGT CTGAACACAC CGCTGAAGAT GAGAATCAAG CCAATAGGAG ACAAGACGAG 
GCTGCTGTGA ACAGTGTGCT CCTCAATCCT GTGTCTCAGC TTTCACTCGC CATCAGGGAA 
AACACCGAAC AGCTCACACA CAAAATCAAG GTTTTGTTTC ATAATCGGAG AGATGTTTTG 
GGTGAAATCG AGGCAAAGAT CAGTGCAGAG GATGATTCTC TATCTATGAA AACCTCAAAC 
AAGGAAGTGG CGTCGATCCT GAAAGAGCTG AGGAGAGTCC AGAAGCAGCT GGAAGTGATC 
AACAGCATCA TGGATCCGCA GGGGAACCCG GAGCTGATGC GGACGTTCTC CTCATCTGCA 
GCTGCAGCGC GTGGATCCAG ATCTGCTCTG CGGGACAACA GATCTGCAGG AGGAGGAGCA 
GGAGCAACAC CCAGAGCCAG ACGCATGTTC AGGAGCACAG ACAGAGACGG CTCGATGGTA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021322929.1
CDS	294..4796
Translation	MSVTSWFLVSSSGTRHRLPREMIFVGREDCELMLQSRSVDKQHAVINYDASTDEHLVKDLGSLNGTFVNDLRIPDQTYIILKLSDVIRFGYDSHIYILERSQHKVPEEALKHEKYTSQLQLETLENKKQLQTGDRRRNSDSTRVKSEKSERKNITDAPISRPTPLYGQPSWWGEDDEDKAQRCDPSSQEQHTENTKEGTKHDLSISPDDLHDGQSKSSFIHKKEPSYFEIPTKENHPKAKSTLTEEIPTKDPDHKPPSVSPVVQGHASFTIEFDEQTPGKMKIKDHISKMSIRQRRNPSKESISRLTEVMSVENKVADWLVQSDTSLRSLEEPRSDQLLNKTFRDAHHNEDPDVEEQEEILSVSHSAAQKTPPQQEPVDYPSNQTADSKNQCRTDPKQAYVIEFFDDAQRKKRSQSFTNNISSPDFQSALKNKLDKRKGTTLSSGHNPATQQFTIPLKDSDSPQRAESLRRETSEIRMSTSEFSSRSVTKPFGSIGRRSKISQDFAAELLRVSKHSAASSWDSNTTWVKTSSALTTNNVVQESLSQSQNHMIRSERVEDGDNTEGKPPKGQEEEDSLSDAGTYTIEAEGPDRDVAEARSLIDQVFGVGENSQTTAASAADKAVVGDSEAHDSLLVNENSSGLIMGQFPSEHSATAKSPAQLQSMTSLTPGGTRWVSRWASLADTYSDSGPASGIFDVPTQVDLPSEAGERINMLNRSADYSENSQNSRSRRVLPQVPLKEKIETPPPMIHIQPDPYLSTVEKSSQVQKHKWDPQKLNVQDDLDPDSLSDASKSDDGSVIEQCRKTPEVNQKTWSRPSKTPSNQDEAKDQDTPQKFSTATLTRQYGKPSKSNGAPPNIGQPSKNDSFGGDSLVSIVRQESYTKERASDDIQLSRLPQISSQPMSKDVFKGVNSQDTHSYLKDTEDVLASLEAKILEQQVNNGRSHPEDSFSGESDTSSTVSGKNPCVSVPKKPIVLKGFLKGTGQYTREPKTSEDQSGMKEKSDPGKRFQLRRNHSSQDFGNEQQSPATRQFPDTVSDQESSSLPYKKYTIPLQKEGTSKSKPTVTRSNSLSAPRPTRASMLRRGRLGEASDNEGTETDRTSQNSDVNPSANNRVAQTSKKLSRLDILALPRKRTGSFNTPSDTESSSASRTSFSNRSTESSSSVRKASVPDPRALLRKVTNAISRQPITRGRSSSAKYASSTASSRRRQKGSDYASTSEDEYESNQSTPKHKRSHHSGAQQSQPAGPVRAMPRSRDSEGEGQESDAFQNWTSHSAEIARLSQDLAKDLAILAQEIHDVAGDTETQSGSQEQTEPAISTQEELRHQIPESSLKVQKESPGAVEESEHTAEDENQANRRQDEAAVNSVLLNPVSQLSLAIRENTEQLTHKIKVLFHNRRDVLGEIEAKISAEDDSLSMKTSNKEVASILKELRRVQKQLEVINSIMDPQGNPELMRTFSSSAAAARGSRSALRDNRSAGGGAGATPRARRMFRSTDRDGSMV

Target ORF information:

RefSeq Version	XM_021467254.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio centrosomal protein 170B (cep170b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021467254.1

ORF Insert Sequence:

ATGAGCGTGA CGTCGTGGTT TCTGGTGAGC AGCTCTGGCA CGCGCCACCG TTTGCCCCGA 
GAGATGATCT TTGTGGGTAG AGAAGACTGT GAACTCATGC TACAGTCGCG CAGTGTGGAC 
AAACAGCACG CTGTGATCAA CTATGATGCA TCTACAGATG AACATTTGGT CAAAGACTTG 
GGCAGCCTCA ACGGGACCTT CGTAAATGAC CTTCGCATCC CCGACCAGAC GTACATCATC 
CTGAAGTTGT CAGATGTGAT CCGCTTTGGT TATGATTCAC ACATATATAT CCTGGAGAGA 
AGCCAACACA AAGTGCCTGA GGAGGCTCTC AAGCATGAAA AATACACCAG TCAGCTTCAG 
CTGGAGACGC TGGAAAACAA GAAGCAGCTT CAGACAGGTG ACAGACGGAG AAACTCAGAC 
TCCACACGGG TCAAATCTGA AAAATCAGAG CGGAAAAACA TCACAGATGC ACCGATTTCC 
AGACCCACAC CTCTTTATGG TCAGCCGTCC TGGTGGGGTG AAGACGATGA AGATAAAGCT 
CAGCGCTGTG ATCCGAGCTC TCAGGAGCAA CATACAGAAA ACACGAAGGA AGGGACCAAA 
CATGATCTCA GCATTTCTCC AGACGATCTT CACGATGGCC AGAGCAAATC CAGCTTCATT 
CACAAAAAGG AGCCCAGTTA TTTTGAGATC CCTACTAAGG AGAACCATCC AAAGGCCAAA 
AGCACATTGA CTGAGGAGAT TCCCACAAAA GACCCCGACC ACAAGCCTCC GTCCGTCTCT 
CCGGTGGTGC AGGGTCACGC CTCCTTCACC ATTGAGTTTG ACGAGCAAAC GCCTGGAAAG 
ATGAAGATTA AGGACCACAT CTCTAAAATG TCCATCCGCC AGCGCAGAAA CCCCAGCAAG 
GAGTCTATAA GCCGCCTGAC GGAGGTGATG TCTGTGGAGA ATAAAGTGGC TGATTGGTTG 
GTGCAGAGCG ACACGTCTTT GAGGAGTCTT GAGGAGCCCA GATCAGATCA GCTGCTCAAC 
AAGACCTTCA GAGATGCACA CCATAATGAA GATCCAGATG TGGAAGAACA GGAGGAAATT 
CTGTCAGTCT CACACTCAGC TGCACAAAAA ACCCCACCAC AACAAGAGCC TGTAGATTAT 
CCATCCAATC AAACGGCAGA TTCAAAGAAC CAATGCAGAA CGGACCCCAA ACAGGCTTAC 
GTGATTGAAT TCTTTGATGA TGCTCAACGC AAAAAGCGCT CGCAGTCTTT CACCAACAAC 
ATCTCCTCTC CAGACTTCCA GTCTGCGCTG AAAAACAAGT TGGACAAACG AAAAGGCACA 
ACTTTGTCAT CCGGACACAA TCCAGCAACA CAACAGTTCA CGATTCCCCT CAAAGATTCA 
GATAGTCCAC AGAGAGCTGA ATCTCTGCGT CGCGAGACGA GCGAAATCCG AATGAGCACT 
TCTGAATTCA GCTCTCGCTC TGTGACAAAA CCGTTCGGCA GCATCGGCCG CAGGTCCAAA 
ATCTCGCAGG ATTTCGCAGC GGAGTTGCTT CGGGTGTCAA AACACAGTGC TGCGTCTTCA 
TGGGACTCCA ATACAACTTG GGTGAAAACG TCTTCGGCAT TAACGACAAA TAATGTGGTG 
CAGGAGAGTC TTTCTCAATC GCAGAATCAC ATGATCAGAT CTGAGCGAGT GGAGGATGGT 
GATAATACTG AGGGCAAACC TCCCAAAGGG CAGGAGGAGG AGGACAGTCT GAGTGATGCA 
GGAACATACA CTATTGAAGC TGAAGGGCCG GATAGAGACG TTGCGGAGGC GCGGAGCTTG 
ATTGATCAGG TGTTTGGTGT TGGAGAAAAC AGCCAAACCA CAGCAGCATC AGCAGCAGAT 
AAGGCTGTAG TTGGTGACTC TGAGGCTCAT GATAGCCTTC TTGTCAATGA GAATAGTTCA 
GGCCTGATAA TGGGTCAGTT CCCATCAGAA CATAGTGCCA CAGCCAAGAG TCCTGCGCAG 
TTACAGTCAA TGACGTCATT AACACCTGGA GGCACTCGAT GGGTTTCTCG CTGGGCCAGT 
TTGGCTGATA CCTATTCAGA CTCTGGTCCC GCTTCAGGAA TATTTGATGT CCCAACACAG 
GTTGATCTTC CCTCCGAAGC TGGTGAAAGG ATAAACATGC TTAACCGGAG CGCTGACTAC 
TCTGAAAATA GCCAAAACAG CAGATCACGA CGAGTTCTGC CTCAGGTTCC TTTAAAGGAG 
AAGATTGAGA CACCTCCACC CATGATACAC ATCCAACCTG ATCCGTACCT GTCCACTGTG 
GAGAAGAGTT CACAAGTTCA AAAGCACAAG TGGGATCCAC AGAAACTCAA TGTGCAGGAT 
GATCTGGATC CAGACAGCCT TAGCGATGCT AGCAAGTCTG ATGACGGATC TGTCATTGAA 
CAGTGCAGGA AGACTCCCGA AGTGAACCAG AAGACATGGA GTCGGCCTTC AAAAACACCA 
TCCAATCAAG ACGAGGCAAA AGACCAGGAT ACACCTCAGA AGTTCTCCAC TGCTACGTTA 
ACCCGACAGT ATGGGAAACC AAGCAAGAGC AATGGTGCAC CACCCAATAT TGGTCAGCCA 
AGCAAAAATG ACTCTTTTGG AGGAGACAGT TTAGTGTCTA TTGTTAGACA AGAAAGTTAC 
ACCAAAGAAA GAGCGAGTGA TGACATCCAG TTGTCCAGAT TGCCCCAGAT ATCGAGTCAG 
CCCATGAGCA AAGATGTTTT TAAGGGTGTA AACAGTCAAG ATACTCATTC ATACCTTAAA 
GACACTGAGG ATGTTCTTGC ATCTCTGGAA GCCAAGATTC TGGAGCAACA AGTAAACAAC 
GGTCGCTCAC ATCCGGAGGA CTCTTTCTCT GGAGAATCCG ACACTTCTAG TACTGTCAGT 
GGCAAAAACC CTTGCGTTTC TGTTCCCAAG AAGCCTATAG TACTCAAAGG ATTCTTAAAA 
GGTACCGGTC AGTATACCAG AGAACCTAAA ACCTCAGAAG ATCAGTCTGG GATGAAGGAG 
AAGTCAGATC CAGGCAAGCG TTTTCAGCTC CGTCGAAATC ATAGTTCTCA AGACTTTGGA 
AATGAACAGC AAAGTCCTGC TACACGGCAG TTTCCTGACA CTGTCTCAGA CCAGGAATCA 
AGTTCTTTAC CTTATAAAAA GTACACTATT CCTCTTCAGA AGGAAGGTAC GAGCAAATCC 
AAACCTACCG TAACTCGCTC CAATAGTCTT TCCGCTCCAA GACCAACAAG AGCGTCAATG 
CTTCGACGTG GACGTTTGGG TGAAGCCTCT GATAATGAAG GTACCGAAAC CGATCGAACG 
TCTCAGAATT CCGACGTCAA TCCTTCTGCA AACAATCGAG TGGCTCAAAC AAGCAAGAAA 
CTTTCTCGAT TGGACATTTT GGCTCTGCCT AGGAAAAGGA CGGGATCTTT TAACACCCCG 
AGTGACACAG AGTCTTCTTC TGCGAGCCGG ACGAGCTTCT CGAACCGCAG CACAGAGTCG 
AGCAGCTCCG TGCGGAAAGC ATCAGTTCCG GATCCCAGGG CTCTTCTGCG TAAGGTTACA 
AATGCCATCA GCAGGCAGCC GATCACTCGC GGACGCTCGA GCAGTGCCAA ATACGCCAGC 
AGCACCGCCA GCTCCAGGAG GCGTCAGAAG GGTTCAGACT ACGCCTCCAC CTCGGAGGAC 
GAGTACGAGT CGAACCAAAG CACTCCTAAA CACAAACGCT CCCATCATTC AGGAGCGCAG 
CAGAGCCAGC CGGCAGGTCC GGTGAGAGCC ATGCCCAGAT CCAGAGACTC TGAGGGGGAA 
GGACAGGAGA GCGACGCCTT CCAGAACTGG ACGTCTCACA GTGCCGAGAT CGCCAGATTG 
AGTCAGGATC TCGCTAAAGA TTTGGCTATT TTGGCCCAAG AGATTCATGA TGTCGCAGGA 
GACACTGAGA CTCAGAGCGG ATCACAAGAG CAGACGGAGC CCGCCATATC CACACAAGAG 
GAGTTGCGGC ATCAGATTCC TGAATCCAGT CTGAAAGTGC AGAAAGAGTC TCCTGGAGCA 
GTCGAAGAGT CTGAACACAC CGCTGAAGAT GAGAATCAAG CCAATAGGAG ACAAGACGAG 
GCTGCTGTGA ACAGTGTGCT CCTCAATCCT GTGTCTCAGC TTTCACTCGC CATCAGGGAA 
AACACCGAAC AGCTCACACA CAAAATCAAG GTTTTGTTTC ATAATCGGAG AGATGTTTTG 
GGTGAAATCG AGGCAAAGAT CAGTGCAGAG GATGATTCTC TATCTATGAA AACCTCAAAC 
AAGGAAGTGG CGTCGATCCT GAAAGAGCTG AGGAGAGTCC AGAAGCAGCT GGAAGTGATC 
AACAGCATCA TGGATCCGCA GGGGAACCCG GAGCTGATGC GGACGTTCTC CTCATCTGCA 
GCTGCAGCGC GTGGATCCAG ATCTGCTCTG CGGGACAACA GATCTGCAGG AGGAGGAGCA 
GGAGCAACAC CCAGAGCCAG ACGCATGTTC AGGAGCACAG ACAGAGACGG CTCGATGGTA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cep170b ( XM_017351731.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017207220.1 Danio rerio centrosomal protein 170B (cep170b), mRNA.

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CloneID	ODa55659
Clone ID Related Accession (Same CDS sequence)	XM_017351731.1
Accession Version	XM_017351731.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4530bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	centrosomal protein of 170 kDa protein B
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

ATGAGCGTGA CGTCGTGGTT TCTGGTGAGC AGCTCTGGCA CGCGCCACCG TTTGCCCCGA 
GAGATGATCT TTGTGGGTAG AGAAGACTGT GAACTCATGC TACAGTCGCG CAGTGTGGAC 
AAACAGCACG CTGTGATCAA CTATGATGCA TCTACAGATG AACATTTGGT CAAAGACTTG 
GGCAGCCTCA ACGGGACCTT CGTAAATGAC CTTCGCATCC CCGACCAGAC GTACATCATC 
CTGAAGTTGT CAGATGTGAT CCGCTTTGGT TATGATTCAC ACATATATAT CCTGGAGAGA 
AGCCAACACA AAGTGCCTGA GGAGGCTCTC AAGCATGAAA AATACACCAG TCAGCTTCAG 
CTGGAGACGC TGGAAAACAA GAAGCAGCTT CAGACAGGTG ACAGACGGAG AAACTCAGAC 
TCCACACGGG TCAAATCTGA AAAATCAGAG CGGAAAAACA TCACAGATGC ACCGATTTCC 
AGACCCACAC CTCTTTATGG TCAGCCGTCC TGGTGGGGTG AAGACGATGA AGATAAAGCT 
CAGCGCTGTG ATCCGAGCTC TCAGGAGCAA CATACAGAAA ACACGAAGGA AGGGACCAAA 
CATGATCTCA GCATTTCTCC AGACGATCTT CACGATGGCC AGAGCAAATC CAGCTTCATT 
CACAAAAAGG AGCCCAGTTA TTTTGAGATC CCTACTAAGG AGAACCATCC AAAGGCCAAA 
AGCACATTGA CTGAGATGCA GGAGATTCCC ACAAAAGACC CCGACCACAA GCCTCCGTCC 
GTCTCTCCGG TGGTGCAGGG TCACGCCTCC TTCACCATTG AGTTTGACGA GCAAACGCCT 
GGAAAGATGA AGATTAAGGA CCACATCTCT AAAATGTCCA TCCGCCAGCG CAGAAACCCC 
AGCAAGGAGT CTATAAGCCG CCTGACGGAG GTGATGTCTG TGGAGAATAA AGTGGCTGAT 
TGGTTGGTGC AGAGCGACAC GTCTTTGAGG AGTCTTGAGG AGCCCAGATC AGATCAGCTG 
CTCAACAAGA CCTTCAGAGA TGCACACCAT AATGAAGATC CAGATGTGGA AGAACAGGAG 
GAAATTCTGT CAGTCTCACA CTCAGCTGCA CAAAAAACCC CACCACAACA AGAGCCTGTA 
GATTATCCAT CCAATCAAAC GGCAGATTCA AAGAACCAAT GCAGAACGGA CCCCAAACAG 
GCTTACGTGA TTGAATTCTT TGATGATGCT CAACGCAAAA AGCGCTCGCA GTCTTTCACC 
AACAACATCT CCTCTCCAGA CTTCCAGTCT GCGCTGAAAA ACAAGTTGGA CAAACGAAAA 
GGCACAACTT TGTCATCCGG ACACAATCCA GCAACACAAC AGTTCACGAT TCCCCTCAAA 
GATTCAGATA GTCCACAGAG AGCTGAATCT CTGCGTCGCG AGACGAGCGA AATCCGAATG 
AGCACTTCTG AATTCAGCTC NNNNNNNNNN GAATTCAGCT CTCGCTCTGT GACAAAACCG 
TTCGGCAGCA TCGGCCGCAG GTCCAAAATC TCGCAGGATT TCGCAGCGGA GTTGCTTCGG 
GTGTCAAAAC ACAGTGCTGC GTCTTCATGG GACTCCAATA CAACTTGGGT GAAAACGTCT 
TCGGCATTAA CGACAAATAA TGTGGTGCAG GAGAGTCTTT CTCAATCGCA GAATCACATG 
ATCAGATCTG AGCGAGTGGA GGATGGTGAT AATACTGAGG GCAAACCTCC CAAAGGGCAG 
GAGGAGGAGG ACAGTCTGAG TGATGCAGGA ACATACACTA TTGAAGCTGA AGGGCCGGAT 
AGAGACGTTG CGGAGGCGCG GAGCTTGATT GATCAGGTGT TTGGTGTTGG AGAAAACAGC 
CAAACCACAG CAGCATCAGC AGCAGATAAG GCTGTAGTTG GTGACTCTGA GGCTCATGAT 
AGCCTTCTTG TCAATGAGAA TAGTTCAGGC CTGATAATGG GTCAGTTCCC ATCAGAACAT 
AGTGCCACAG CCAAGAGTCC TGCGCAGTTA CAGTCAATGA CGTCATTAAC ACCTGGAGGC 
ACTCGATGGG TTTCTCGCTG GGCCAGTTTG GCTGATACCT ATTCAGACTC TGGTCCCGCT 
TCAGGAATAT TTGATGTCCC AACACAGGTT GATCTTCCCT CCGAAGCTGG TGAAAGGATA 
AACATGCTTA ACCGGAGCGC TGACTACTCT GAAAATAGCC AAAACAGCAG ATCACGACGA 
GTTCTGCCTC AGGTTCCTTT AAAGGAGAAG ATTGAGACAC CTCCACCCAT GATACACATC 
CAACCTGATC CGTACCTGTC CACTGTGGAG AAGAGTTCAC AAGTTCAAAA GCACAAGTGG 
GATCCACAGA AACTCAATGT GCAGGATGAT CTGGATCCAG ACAGCCTTAG CGATGCTAGC 
AAGTCTGATG ACGGATCTGT CATTGAACAG TGCAGGAAGA CTCCCGAAGT GAACCAGAAG 
ACATGGAGTC GGCCTTCAAA AACACCATCC AATCAAGACG AGGCAAAAGA CCAGGATACA 
CCTCAGAAGT TCTCCACTGC TACGTTAACC CGACAGTATG GGAAACCAAG CAAGAGCAAT 
GGTGCACCAC CCAATATTGG TCAGCCAAGC AAAAATGACT CTTTTGGAGG AGACAGTTTA 
GTGTCTATTG TTAGACAAGA AAGTTACACC AAAGAAAGAG CGAGTGATGA CATCCAGTTG 
TCCAGATTGC CCCAGATATC GAGTCAGCCC ATGAGCAAAG ATGTTTTTAA GGGTGTAAAC 
AGTCAAGATA CTCATTCATA CCTTAAAGAC ACTGAGGATG TTCTTGCATC TCTGGAAGCC 
AAGATTCTGG AGCAACAAGT AAACAACGGT CGCTCACATC CGGAGGACTC TTTCTCTGGA 
GAATCCGACA CTTCTAGTAC TGTCAGTGGC AAAAACCCTT GCGTTTCTGT TCCCAAGAAG 
CCTATAGTAC TCAAAGGATT CTTAAAAGGT ACCGGTCAGT ATACCAGAGA ACCTAAAACC 
TCAGAAGATC AGTCTGGGAT GAAGGAGAAG TCAGATCCAG GCAAGCGTTT TCAGCTCCGT 
CGAAATCATA GTTCTCAAGA CTTTGGAAAT GAACAGCAAA GTCCTGCTAC ACGGCAGTTT 
CCTGACACTG TCTCAGACCA GGAATCAAGT TCTTTACCTT ATAAAAAGTA CACTATTCCT 
CTTCAGAAGG AAGGTACGAG CAAATCCAAA CCTACCGTAA CTCGCTCCAA TAGTCTTTCC 
GCTCCAAGAC CAACAAGAGC GTCAATGCTT CGACGTGGAC GTTTGGGTGA AGCCTCTGAT 
AATGAAGGTA CCGAAACCGA TCGAACGTCT CAGAATTCCG ACGTCAATCC TTCTGCAAAC 
AATCGAGTGG CTCAAACAAG CAAGAAACTT TCTCGATTGG ACATTTTGGC TCTGCCTAGG 
AAAAGGACGG GATCTTTTAA CACCCCGAGT GACACAGAGT CTTCTTCTGC GAGCCGGACG 
AGCTTCTCGA ACCGCAGCAC AGAGTCGAGC AGCTCCGTGC GGAAAGCATC AGTTCCGGAT 
CCCAGGGCTC TTCTGCGTAA GGTTACAAAT GCCATCAGCA GGCAGCCGAT CACTCGCGGA 
CGCTCGAGCA GTGCCAAATA CGCCAGCAGC ACCGCCAGCT CCAGGAGGCG TCAGAAGGGT 
TCAGACTACG CCTCCACCTC GGAGGACGAG TACGAGTCGA ACCAAAGCAC TCCTAAACAC 
AAACGCTCCC ATCATTCAGG AGCGCAGCAG AGCCAGCCGG CAGGTCCGGT GAGAGCCATG 
CCCAGATCCA GAGACTCTGA GGGGGAAGGA CAGGAGAGCG ACGCCTTCCA GAACTGGACG 
TCTCACAGTG CCGAGATCGC CAGATTGAGT CAGGATCTCG CTAAAGATTT GGCTATTTTG 
GCCCAAGAGA TTCATGATGT CGCAGGAGAC ACTGAGACTC AGAGCGGATC ACAAGAGCAG 
ACGGAGCCCG CCATATCCAC ACAAGAGGAG TTGCGGCATC AGATTCCTGA ATCCAGTCTG 
AAAGTGCAGA AAGAGTCTCC TGGAGCAGTC GAAGAGTCTG AACACACCGC TGAAGATGAG 
AATCAAGCCA ATAGGAGACA AGACGAGGCT GCTGTGAACA GTGTGCTCCT CAATCCTGTG 
TCTCAGCTTT CACTCGCCAT CAGGGAAAAC ACCGAACAGC TCACACACAA AATCAAGGTT 
TTGTTTCATA ATCGGAGAGA TGTTTTGGGT GAAATCGAGG CAAAGATCAG TGCAGAGGAT 
GATTCTCTAT CTATGAAAAC CTCAAACAAG GAAGTGGCGT CGATCCTGAA AGAGCTGAGG 
AGAGTCCAGA AGCAGCTGGA AGTGATCAAC AGCATCATGG ATCCGCAGGG GAACCCGGAG 
CTGATGCGGA CGTTCTCCTC ATCTGCAGCT GCAGCGCGTG GATCCAGATC TGCTCTGCGG 
GACAACAGAT CTGCAGGAGG AGGAGCAGGA GCAACACCCA GAGCCAGACG CATGTTCAGG 
AGCACAGACA GAGACGGCTC GATGGTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017207220.1
CDS	315..4844
Translation	MSVTSWFLVSSSGTRHRLPREMIFVGREDCELMLQSRSVDKQHAVINYDASTDEHLVKDLGSLNGTFVNDLRIPDQTYIILKLSDVIRFGYDSHIYILERSQHKVPEEALKHEKYTSQLQLETLENKKQLQTGDRRRNSDSTRVKSEKSERKNITDAPISRPTPLYGQPSWWGEDDEDKAQRCDPSSQEQHTENTKEGTKHDLSISPDDLHDGQSKSSFIHKKEPSYFEIPTKENHPKAKSTLTEMQEIPTKDPDHKPPSVSPVVQGHASFTIEFDEQTPGKMKIKDHISKMSIRQRRNPSKESISRLTEVMSVENKVADWLVQSDTSLRSLEEPRSDQLLNKTFRDAHHNEDPDVEEQEEILSVSHSAAQKTPPQQEPVDYPSNQTADSKNQCRTDPKQAYVIEFFDDAQRKKRSQSFTNNISSPDFQSALKNKLDKRKGTTLSSGHNPATQQFTIPLKDSDSPQRAESLRRETSEIRMSTSEFSnullnullnullnullEFSSRSVTKPFGSIGRRSKISQDFAAELLRVSKHSAASSWDSNTTWVKTSSALTTNNVVQESLSQSQNHMIRSERVEDGDNTEGKPPKGQEEEDSLSDAGTYTIEAEGPDRDVAEARSLIDQVFGVGENSQTTAASAADKAVVGDSEAHDSLLVNENSSGLIMGQFPSEHSATAKSPAQLQSMTSLTPGGTRWVSRWASLADTYSDSGPASGIFDVPTQVDLPSEAGERINMLNRSADYSENSQNSRSRRVLPQVPLKEKIETPPPMIHIQPDPYLSTVEKSSQVQKHKWDPQKLNVQDDLDPDSLSDASKSDDGSVIEQCRKTPEVNQKTWSRPSKTPSNQDEAKDQDTPQKFSTATLTRQYGKPSKSNGAPPNIGQPSKNDSFGGDSLVSIVRQESYTKERASDDIQLSRLPQISSQPMSKDVFKGVNSQDTHSYLKDTEDVLASLEAKILEQQVNNGRSHPEDSFSGESDTSSTVSGKNPCVSVPKKPIVLKGFLKGTGQYTREPKTSEDQSGMKEKSDPGKRFQLRRNHSSQDFGNEQQSPATRQFPDTVSDQESSSLPYKKYTIPLQKEGTSKSKPTVTRSNSLSAPRPTRASMLRRGRLGEASDNEGTETDRTSQNSDVNPSANNRVAQTSKKLSRLDILALPRKRTGSFNTPSDTESSSASRTSFSNRSTESSSSVRKASVPDPRALLRKVTNAISRQPITRGRSSSAKYASSTASSRRRQKGSDYASTSEDEYESNQSTPKHKRSHHSGAQQSQPAGPVRAMPRSRDSEGEGQESDAFQNWTSHSAEIARLSQDLAKDLAILAQEIHDVAGDTETQSGSQEQTEPAISTQEELRHQIPESSLKVQKESPGAVEESEHTAEDENQANRRQDEAAVNSVLLNPVSQLSLAIRENTEQLTHKIKVLFHNRRDVLGEIEAKISAEDDSLSMKTSNKEVASILKELRRVQKQLEVINSIMDPQGNPELMRTFSSSAAAARGSRSALRDNRSAGGGAGATPRARRMFRSTDRDGSMV

Target ORF information:

RefSeq Version	XM_017351731.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio centrosomal protein 170B (cep170b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017351731.1

ORF Insert Sequence:

ATGAGCGTGA CGTCGTGGTT TCTGGTGAGC AGCTCTGGCA CGCGCCACCG TTTGCCCCGA 
GAGATGATCT TTGTGGGTAG AGAAGACTGT GAACTCATGC TACAGTCGCG CAGTGTGGAC 
AAACAGCACG CTGTGATCAA CTATGATGCA TCTACAGATG AACATTTGGT CAAAGACTTG 
GGCAGCCTCA ACGGGACCTT CGTAAATGAC CTTCGCATCC CCGACCAGAC GTACATCATC 
CTGAAGTTGT CAGATGTGAT CCGCTTTGGT TATGATTCAC ACATATATAT CCTGGAGAGA 
AGCCAACACA AAGTGCCTGA GGAGGCTCTC AAGCATGAAA AATACACCAG TCAGCTTCAG 
CTGGAGACGC TGGAAAACAA GAAGCAGCTT CAGACAGGTG ACAGACGGAG AAACTCAGAC 
TCCACACGGG TCAAATCTGA AAAATCAGAG CGGAAAAACA TCACAGATGC ACCGATTTCC 
AGACCCACAC CTCTTTATGG TCAGCCGTCC TGGTGGGGTG AAGACGATGA AGATAAAGCT 
CAGCGCTGTG ATCCGAGCTC TCAGGAGCAA CATACAGAAA ACACGAAGGA AGGGACCAAA 
CATGATCTCA GCATTTCTCC AGACGATCTT CACGATGGCC AGAGCAAATC CAGCTTCATT 
CACAAAAAGG AGCCCAGTTA TTTTGAGATC CCTACTAAGG AGAACCATCC AAAGGCCAAA 
AGCACATTGA CTGAGATGCA GGAGATTCCC ACAAAAGACC CCGACCACAA GCCTCCGTCC 
GTCTCTCCGG TGGTGCAGGG TCACGCCTCC TTCACCATTG AGTTTGACGA GCAAACGCCT 
GGAAAGATGA AGATTAAGGA CCACATCTCT AAAATGTCCA TCCGCCAGCG CAGAAACCCC 
AGCAAGGAGT CTATAAGCCG CCTGACGGAG GTGATGTCTG TGGAGAATAA AGTGGCTGAT 
TGGTTGGTGC AGAGCGACAC GTCTTTGAGG AGTCTTGAGG AGCCCAGATC AGATCAGCTG 
CTCAACAAGA CCTTCAGAGA TGCACACCAT AATGAAGATC CAGATGTGGA AGAACAGGAG 
GAAATTCTGT CAGTCTCACA CTCAGCTGCA CAAAAAACCC CACCACAACA AGAGCCTGTA 
GATTATCCAT CCAATCAAAC GGCAGATTCA AAGAACCAAT GCAGAACGGA CCCCAAACAG 
GCTTACGTGA TTGAATTCTT TGATGATGCT CAACGCAAAA AGCGCTCGCA GTCTTTCACC 
AACAACATCT CCTCTCCAGA CTTCCAGTCT GCGCTGAAAA ACAAGTTGGA CAAACGAAAA 
GGCACAACTT TGTCATCCGG ACACAATCCA GCAACACAAC AGTTCACGAT TCCCCTCAAA 
GATTCAGATA GTCCACAGAG AGCTGAATCT CTGCGTCGCG AGACGAGCGA AATCCGAATG 
AGCACTTCTG AATTCAGCTC NNNNNNNNNN GAATTCAGCT CTCGCTCTGT GACAAAACCG 
TTCGGCAGCA TCGGCCGCAG GTCCAAAATC TCGCAGGATT TCGCAGCGGA GTTGCTTCGG 
GTGTCAAAAC ACAGTGCTGC GTCTTCATGG GACTCCAATA CAACTTGGGT GAAAACGTCT 
TCGGCATTAA CGACAAATAA TGTGGTGCAG GAGAGTCTTT CTCAATCGCA GAATCACATG 
ATCAGATCTG AGCGAGTGGA GGATGGTGAT AATACTGAGG GCAAACCTCC CAAAGGGCAG 
GAGGAGGAGG ACAGTCTGAG TGATGCAGGA ACATACACTA TTGAAGCTGA AGGGCCGGAT 
AGAGACGTTG CGGAGGCGCG GAGCTTGATT GATCAGGTGT TTGGTGTTGG AGAAAACAGC 
CAAACCACAG CAGCATCAGC AGCAGATAAG GCTGTAGTTG GTGACTCTGA GGCTCATGAT 
AGCCTTCTTG TCAATGAGAA TAGTTCAGGC CTGATAATGG GTCAGTTCCC ATCAGAACAT 
AGTGCCACAG CCAAGAGTCC TGCGCAGTTA CAGTCAATGA CGTCATTAAC ACCTGGAGGC 
ACTCGATGGG TTTCTCGCTG GGCCAGTTTG GCTGATACCT ATTCAGACTC TGGTCCCGCT 
TCAGGAATAT TTGATGTCCC AACACAGGTT GATCTTCCCT CCGAAGCTGG TGAAAGGATA 
AACATGCTTA ACCGGAGCGC TGACTACTCT GAAAATAGCC AAAACAGCAG ATCACGACGA 
GTTCTGCCTC AGGTTCCTTT AAAGGAGAAG ATTGAGACAC CTCCACCCAT GATACACATC 
CAACCTGATC CGTACCTGTC CACTGTGGAG AAGAGTTCAC AAGTTCAAAA GCACAAGTGG 
GATCCACAGA AACTCAATGT GCAGGATGAT CTGGATCCAG ACAGCCTTAG CGATGCTAGC 
AAGTCTGATG ACGGATCTGT CATTGAACAG TGCAGGAAGA CTCCCGAAGT GAACCAGAAG 
ACATGGAGTC GGCCTTCAAA AACACCATCC AATCAAGACG AGGCAAAAGA CCAGGATACA 
CCTCAGAAGT TCTCCACTGC TACGTTAACC CGACAGTATG GGAAACCAAG CAAGAGCAAT 
GGTGCACCAC CCAATATTGG TCAGCCAAGC AAAAATGACT CTTTTGGAGG AGACAGTTTA 
GTGTCTATTG TTAGACAAGA AAGTTACACC AAAGAAAGAG CGAGTGATGA CATCCAGTTG 
TCCAGATTGC CCCAGATATC GAGTCAGCCC ATGAGCAAAG ATGTTTTTAA GGGTGTAAAC 
AGTCAAGATA CTCATTCATA CCTTAAAGAC ACTGAGGATG TTCTTGCATC TCTGGAAGCC 
AAGATTCTGG AGCAACAAGT AAACAACGGT CGCTCACATC CGGAGGACTC TTTCTCTGGA 
GAATCCGACA CTTCTAGTAC TGTCAGTGGC AAAAACCCTT GCGTTTCTGT TCCCAAGAAG 
CCTATAGTAC TCAAAGGATT CTTAAAAGGT ACCGGTCAGT ATACCAGAGA ACCTAAAACC 
TCAGAAGATC AGTCTGGGAT GAAGGAGAAG TCAGATCCAG GCAAGCGTTT TCAGCTCCGT 
CGAAATCATA GTTCTCAAGA CTTTGGAAAT GAACAGCAAA GTCCTGCTAC ACGGCAGTTT 
CCTGACACTG TCTCAGACCA GGAATCAAGT TCTTTACCTT ATAAAAAGTA CACTATTCCT 
CTTCAGAAGG AAGGTACGAG CAAATCCAAA CCTACCGTAA CTCGCTCCAA TAGTCTTTCC 
GCTCCAAGAC CAACAAGAGC GTCAATGCTT CGACGTGGAC GTTTGGGTGA AGCCTCTGAT 
AATGAAGGTA CCGAAACCGA TCGAACGTCT CAGAATTCCG ACGTCAATCC TTCTGCAAAC 
AATCGAGTGG CTCAAACAAG CAAGAAACTT TCTCGATTGG ACATTTTGGC TCTGCCTAGG 
AAAAGGACGG GATCTTTTAA CACCCCGAGT GACACAGAGT CTTCTTCTGC GAGCCGGACG 
AGCTTCTCGA ACCGCAGCAC AGAGTCGAGC AGCTCCGTGC GGAAAGCATC AGTTCCGGAT 
CCCAGGGCTC TTCTGCGTAA GGTTACAAAT GCCATCAGCA GGCAGCCGAT CACTCGCGGA 
CGCTCGAGCA GTGCCAAATA CGCCAGCAGC ACCGCCAGCT CCAGGAGGCG TCAGAAGGGT 
TCAGACTACG CCTCCACCTC GGAGGACGAG TACGAGTCGA ACCAAAGCAC TCCTAAACAC 
AAACGCTCCC ATCATTCAGG AGCGCAGCAG AGCCAGCCGG CAGGTCCGGT GAGAGCCATG 
CCCAGATCCA GAGACTCTGA GGGGGAAGGA CAGGAGAGCG ACGCCTTCCA GAACTGGACG 
TCTCACAGTG CCGAGATCGC CAGATTGAGT CAGGATCTCG CTAAAGATTT GGCTATTTTG 
GCCCAAGAGA TTCATGATGT CGCAGGAGAC ACTGAGACTC AGAGCGGATC ACAAGAGCAG 
ACGGAGCCCG CCATATCCAC ACAAGAGGAG TTGCGGCATC AGATTCCTGA ATCCAGTCTG 
AAAGTGCAGA AAGAGTCTCC TGGAGCAGTC GAAGAGTCTG AACACACCGC TGAAGATGAG 
AATCAAGCCA ATAGGAGACA AGACGAGGCT GCTGTGAACA GTGTGCTCCT CAATCCTGTG 
TCTCAGCTTT CACTCGCCAT CAGGGAAAAC ACCGAACAGC TCACACACAA AATCAAGGTT 
TTGTTTCATA ATCGGAGAGA TGTTTTGGGT GAAATCGAGG CAAAGATCAG TGCAGAGGAT 
GATTCTCTAT CTATGAAAAC CTCAAACAAG GAAGTGGCGT CGATCCTGAA AGAGCTGAGG 
AGAGTCCAGA AGCAGCTGGA AGTGATCAAC AGCATCATGG ATCCGCAGGG GAACCCGGAG 
CTGATGCGGA CGTTCTCCTC ATCTGCAGCT GCAGCGCGTG GATCCAGATC TGCTCTGCGG 
GACAACAGAT CTGCAGGAGG AGGAGCAGGA GCAACACCCA GAGCCAGACG CATGTTCAGG 
AGCACAGACA GAGACGGCTC GATGGTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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cep170b cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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