parn cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	parn
Entrez Gene ID	791461
Full Name	poly(A)-specific ribonuclease (deadenylation nuclease)
Synonyms	zgc:56067
General protein information	Preferred Names poly(A)-specific ribonuclease (deadenylation nuclease) Names poly(A)-specific ribonuclease PARN polyadenylate-specific ribonuclease
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001320404.1	NP_001307333.1	poly(A)-specific ribonuclease PARN isoform 1
NM_201088.2	NP_957382.2	poly(A)-specific ribonuclease PARN isoform 2

Pathways from BioSystems

Homologies

Homo sapiens (human)	PARN	NP_002573.1
Canis lupus familiaris (dog)	PARN	XP_536966.2
Rattus norvegicus (Norway rat)	Parn	NP_001257344.1
Arabidopsis thaliana (thale cress)	AHG2	NP_175983.5
	LOC712984	XP_002808334.1
Bos taurus (cattle)	PARN	NP_001094588.1
Danio rerio (zebrafish)	parn	NP_957382.1
Anopheles gambiae (African malaria mosquito)	PARN	XP_308433.4
Caenorhabditis elegans (roundworm)	K10C8.1	NP_506169.2
Schizosaccharomyces pombe (fission yeast)	SPBC29A10.09c	NP_596054.1
Gallus gallus (chicken)	PARN	NP_001025800.1
Pan troglodytes (chimpanzee)	PARN	XP_510832.3
Mus musculus (house mouse)	Parn	NP_083037.1
Xenopus tropicalis (tropical clawed frog)	parn	NP_001184102.1

Gene Ontology
Bibliography

Related articles in PubMed

Protein palmitoylation activate zygotic gene expression during the maternal-to-zygotic transition.
Du Z, Chen X, Li X, He K, Ji S, Shi W, Hao A
Biochemical and biophysical research communications475(2)194-201(2016 06)

RNA structure replaces the need for U2AF2 in splicing.
Lin CL, Taggart AJ, Lim KH, Cygan KJ, Ferraris L, Creton R, Huang YT, Fairbrother WG
Genome research26(1)12-23(2016 Jan)

Bone marrow failure and developmental delay caused by mutations in poly(A)-specific ribonuclease (PARN).
Dhanraj S, Gunja SM, Deveau AP, Nissbeck M, Boonyawat B, Coombs AJ, Renieri A, Mucciolo M, Marozza A, Buoni S, Turner L, Li H, Jarrar A, Sabanayagam M, Kirby M, Shago M, Pinto D, Berman JN, Scherer SW, Virtanen A, Dror Y
Journal of medical genetics52(11)738-48(2015 Nov)

Online automated in vivo zebrafish phosphoproteomics: from large-scale analysis down to a single embryo.
Lemeer S, Pinkse MW, Mohammed S, van Breukelen B, den Hertog J, Slijper M, Heck AJ
Journal of proteome research7(4)1555-64(2008 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following parn gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the parn cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa98486	NM_201088.2 Latest version!	Danio rerio poly(A)-specific ribonuclease (deadenylation nuclease) (parn), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
ODa98501	NM_001320404.1 Latest version!	Danio rerio poly(A)-specific ribonuclease (deadenylation nuclease) (parn), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

parn ( NM_201088.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_957382.2 Danio rerio poly(A)-specific ribonuclease (deadenylation nuclease) (parn), transcript variant 2, mRNA.

Price & Availability↑

CloneID	ODa98486
Clone ID Related Accession (Same CDS sequence)	NM_201088.2
Accession Version	NM_201088.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1944bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-15
Organism	Danio rerio(zebrafish)
Product	poly(A)-specific ribonuclease PARN isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CU550701.5, CN503632.1 and EL650012.1. On Feb 24, 2016 this sequence version replaced NM_201088.1. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 3' coding region compared to variant 1. It encodes isoform 2, which is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: BC050496.1, GDQH01008238.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGGAGGTCA CGAGACAGAA TTTTAAGGAG ATTCTTCCAG AGGTGTGTAA CGCGGTGCAG 
GAGGCGGATT TCATCTCTAT CGACGGAGAG TTCACAGGAA TCAGTGATGG GCCGTCTGTC 
AGTGCTCTGA CCAATGGACT GGACACTCCA GAGGAGAGAT ACACCAAACT GAGGAAGCAC 
TCCATGAACT TCCTGCTGTT CCAGTTCGGC GTCTGCACCT TCAGATACGA CCAGAACCAG 
TCGACGTACA TCACTAAAGC CTTCAACTTC TACATCTTCC CCAAACCCTT CAGCCGCACG 
TCTCCAGATA TAAAGTTCAT CTGTCAGAGC TCCAGTATTG ACTTTCTGGC CAGTCAGGGC 
TTCGACTTCA ACAAGGTCTT CCGCAGCGGG ATCCCATACC TAACTCAGGA GGAGGAGTGT 
CAGCTGAGGG AGCAGTATGA GGAGAGGCGG GGTCAGATGA ATGGGGCGGG CCCAGTCTCA 
TACACGCCCC CTTCAGGCAC TGGTGTGTGT AACGTGCCAG AGGACCAGAG AGAGTTCATC 
AGGAGTGTGG AGGAAAAGGT TGAAGCTCTA CTAAAGAACA CTGATCAGAC CCTGGATCTG 
GAGCCCTGCA CCGGGTTTCA GAGGAAGCTC ATCTATCAGA CACTCAACTC AAAGTATTCT 
AAAGGTCTTC ATGTGGAGAC GCTGGAGACT GAGAAGAAGG AGCGTTTCAT CCAGATCAGT 
AAAGTGGATG ATGAGGAGAG GAGGAGGAGA GAGCAGCAGA AACAGCAGAG AGAGCAGGAG 
GAGCTGAATG ATGCTGTGGG TTTCTCCAGG GTTATCAGAG CCATCTCCAA ATCTGGTAAG 
CTGGTGGTGG GCCACAACAT GCTGCTGGAC GTCATGCACA CCATACACCA GTTCTGCGGG 
CCGCTGCCGG AGGAGCTGGA TGACTTCAAG GAGGTGGCCA TGACTGTGTT TCCCAGGCTG 
CTAGACACCA AGCTGATGGC GTCCACACAA CCGTTCAAGG AGATCATCCA CAACACCTCA 
CTGGCTGAAC TACACAAACA GCTGAGACAG AAACCCTTCA GACCACCCAC CACTGAGTGT 
CCTGAAGGCC TCCAGAGTTA CGACACCTCC ACCGAGCAGC TTCATGAAGC TGGATATGAC 
GCCTTCATCA CCGGACTCTG CTTCATCTCC ATGGCCAACT ACCTGGGCTC TTTCCTCACG 
CCGCCCAAGA GCCACATCTC TGCCCGCTCC AAACTGCTGG AGCCCTTCTA CAACAAATTA 
TTCCTGATGA GGGTCATCGA CATTCCCTAC CTGAACATGA GCGGACCTGA CTTGCAGCCC 
AAGAGAGATC ATGTCCTGTA CGTCACGTTC CCTAAAGAGT GGAAGACCAG TGACCTGTAC 
CAGCTGTTCA GCGCCTTCGG GAACATCCAG GTGTCGTGGG TGGACGACAC GTCAGCGTTC 
GTGTCTCTCA GTCAGACAGA GCAGGTTCAG ATCGCCATGA ACACCAGCCG ATACGCTGAG 
AGCTACCGCA TCCAGACCTA TGCGGAGTAC CTGCAGTCCC GGCAGAAGAA CACACACTCC 
AGCAGGAAGT GGGCCAGCGA TGGGTGGGCG GATACTTCCT ACCCCAGTGT CGCCATGACA 
ACCGCATCCG GCTACAGCCA CACCGACAAC CGGCATCAGG CGGTCAAGCG CAGCATCAGT 
CCGTCTCTGG ATGAGCAGAA TCACGGCGCC GACAGCAGCT GGACGAACTA CAGCGTCAAG 
AAGATCAAGA CTGAGGGAAG CTGCACGCAG ACCTACGCTG ACGTCGCCGG CTCCTGTGAT 
TGGCCTAGAC TGCAGGCTGA TGAAGGCGGA GCCTCTGTGA GCCCAGTGGC AGAGGAGGCG 
GAGCTCGATG AGTTCTCAGC CAATCAGAGT CAAGCTTCTG AAACTACACC GCCAGCGCTC 
TTTGACGTCC CTCAGGTTTG GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_957382.2
CDS	76..2019
Translation	MEVTRQNFKEILPEVCNAVQEADFISIDGEFTGISDGPSVSALTNGLDTPEERYTKLRKHSMNFLLFQFGVCTFRYDQNQSTYITKAFNFYIFPKPFSRTSPDIKFICQSSSIDFLASQGFDFNKVFRSGIPYLTQEEECQLREQYEERRGQMNGAGPVSYTPPSGTGVCNVPEDQREFIRSVEEKVEALLKNTDQTLDLEPCTGFQRKLIYQTLNSKYSKGLHVETLETEKKERFIQISKVDDEERRRREQQKQQREQEELNDAVGFSRVIRAISKSGKLVVGHNMLLDVMHTIHQFCGPLPEELDDFKEVAMTVFPRLLDTKLMASTQPFKEIIHNTSLAELHKQLRQKPFRPPTTECPEGLQSYDTSTEQLHEAGYDAFITGLCFISMANYLGSFLTPPKSHISARSKLLEPFYNKLFLMRVIDIPYLNMSGPDLQPKRDHVLYVTFPKEWKTSDLYQLFSAFGNIQVSWVDDTSAFVSLSQTEQVQIAMNTSRYAESYRIQTYAEYLQSRQKNTHSSRKWASDGWADTSYPSVAMTTASGYSHTDNRHQAVKRSISPSLDEQNHGADSSWTNYSVKKIKTEGSCTQTYADVAGSCDWPRLQADEGGASVSPVAEEAELDEFSANQSQASETTPPALFDVPQVW

Target ORF information:

RefSeq Version	NM_201088.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio poly(A)-specific ribonuclease (deadenylation nuclease) (parn), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_201088.2

ORF Insert Sequence:

ATGGAGGTCA CGAGACAGAA TTTTAAGGAG ATTCTTCCAG AGGTGTGTAA CGCGGTGCAG 
GAGGCGGATT TCATCTCTAT CGACGGAGAG TTCACAGGAA TCAGTGATGG GCCGTCTGTC 
AGTGCTCTGA CCAATGGACT GGACACTCCA GAGGAGAGAT ACACCAAACT GAGGAAGCAC 
TCCATGAACT TCCTGCTGTT CCAGTTCGGC GTCTGCACCT TCAGATACGA CCAGAACCAG 
TCGACGTACA TCACTAAAGC CTTCAACTTC TACATCTTCC CCAAACCCTT CAGCCGCACG 
TCTCCAGATA TAAAGTTCAT CTGTCAGAGC TCCAGTATTG ACTTTCTGGC CAGTCAGGGC 
TTCGACTTCA ACAAGGTCTT CCGCAGCGGG ATCCCATACC TAACTCAGGA GGAGGAGTGT 
CAGCTGAGGG AGCAGTATGA GGAGAGGCGG GGTCAGATGA ATGGGGCGGG CCCAGTCTCA 
TACACGCCCC CTTCAGGCAC TGGTGTGTGT AACGTGCCAG AGGACCAGAG AGAGTTCATC 
AGGAGTGTGG AGGAAAAGGT TGAAGCTCTA CTAAAGAACA CTGATCAGAC CCTGGATCTG 
GAGCCCTGCA CCGGGTTTCA GAGGAAGCTC ATCTATCAGA CACTCAACTC AAAGTATTCT 
AAAGGTCTTC ATGTGGAGAC GCTGGAGACT GAGAAGAAGG AGCGTTTCAT CCAGATCAGT 
AAAGTGGATG ATGAGGAGAG GAGGAGGAGA GAGCAGCAGA AACAGCAGAG AGAGCAGGAG 
GAGCTGAATG ATGCTGTGGG TTTCTCCAGG GTTATCAGAG CCATCTCCAA ATCTGGTAAG 
CTGGTGGTGG GCCACAACAT GCTGCTGGAC GTCATGCACA CCATACACCA GTTCTGCGGG 
CCGCTGCCGG AGGAGCTGGA TGACTTCAAG GAGGTGGCCA TGACTGTGTT TCCCAGGCTG 
CTAGACACCA AGCTGATGGC GTCCACACAA CCGTTCAAGG AGATCATCCA CAACACCTCA 
CTGGCTGAAC TACACAAACA GCTGAGACAG AAACCCTTCA GACCACCCAC CACTGAGTGT 
CCTGAAGGCC TCCAGAGTTA CGACACCTCC ACCGAGCAGC TTCATGAAGC TGGATATGAC 
GCCTTCATCA CCGGACTCTG CTTCATCTCC ATGGCCAACT ACCTGGGCTC TTTCCTCACG 
CCGCCCAAGA GCCACATCTC TGCCCGCTCC AAACTGCTGG AGCCCTTCTA CAACAAATTA 
TTCCTGATGA GGGTCATCGA CATTCCCTAC CTGAACATGA GCGGACCTGA CTTGCAGCCC 
AAGAGAGATC ATGTCCTGTA CGTCACGTTC CCTAAAGAGT GGAAGACCAG TGACCTGTAC 
CAGCTGTTCA GCGCCTTCGG GAACATCCAG GTGTCGTGGG TGGACGACAC GTCAGCGTTC 
GTGTCTCTCA GTCAGACAGA GCAGGTTCAG ATCGCCATGA ACACCAGCCG ATACGCTGAG 
AGCTACCGCA TCCAGACCTA TGCGGAGTAC CTGCAGTCCC GGCAGAAGAA CACACACTCC 
AGCAGGAAGT GGGCCAGCGA TGGGTGGGCG GATACTTCCT ACCCCAGTGT CGCCATGACA 
ACCGCATCCG GCTACAGCCA CACCGACAAC CGGCATCAGG CGGTCAAGCG CAGCATCAGT 
CCGTCTCTGG ATGAGCAGAA TCACGGCGCC GACAGCAGCT GGACGAACTA CAGCGTCAAG 
AAGATCAAGA CTGAGGGAAG CTGCACGCAG ACCTACGCTG ACGTCGCCGG CTCCTGTGAT 
TGGCCTAGAC TGCAGGCTGA TGAAGGCGGA GCCTCTGTGA GCCCAGTGGC AGAGGAGGCG 
GAGCTCGATG AGTTCTCAGC CAATCAGAGT CAAGCTTCTG AAACTACACC GCCAGCGCTC 
TTTGACGTCC CTCAGGTTTG GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

parn ( NM_001320404.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001307333.1 Danio rerio poly(A)-specific ribonuclease (deadenylation nuclease) (parn), transcript variant 1, mRNA.

Price & Availability↑

CloneID	ODa98501
Clone ID Related Accession (Same CDS sequence)	NM_001320404.1
Accession Version	NM_001320404.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1983bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-15
Organism	Danio rerio(zebrafish)
Product	poly(A)-specific ribonuclease PARN isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CU550701.5, CN503632.1 and EL650012.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AY398393.1, GFIL01029489.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGGAGGTCA CGAGACAGAA TTTTAAGGAG ATTCTTCCAG AGGTGTGTAA CGCGGTGCAG 
GAGGCGGATT TCATCTCTAT CGACGGAGAG TTCACAGGAA TCAGTGATGG GCCGTCTGTC 
AGTGCTCTGA CCAATGGACT GGACACTCCA GAGGAGAGAT ACACCAAACT GAGGAAGCAC 
TCCATGAACT TCCTGCTGTT CCAGTTCGGC GTCTGCACCT TCAGATACGA CCAGAACCAG 
TCGACGTACA TCACTAAAGC CTTCAACTTC TACATCTTCC CCAAACCCTT CAGCCGCACG 
TCTCCAGATA TAAAGTTCAT CTGTCAGAGC TCCAGTATTG ACTTTCTGGC CAGTCAGGGC 
TTCGACTTCA ACAAGGTCTT CCGCAGCGGG ATCCCATACC TAACTCAGGA GGAGGAGTGT 
CAGCTGAGGG AGCAGTATGA GGAGAGGCGG GGTCAGATGA ATGGGGCGGG CCCAGTCTCA 
TACACGCCCC CTTCAGGCAC TGGTGTGTGT AACGTGCCAG AGGACCAGAG AGAGTTCATC 
AGGAGTGTGG AGGAAAAGGT TGAAGCTCTA CTAAAGAACA CTGATCAGAC CCTGGATCTG 
GAGCCCTGCA CCGGGTTTCA GAGGAAGCTC ATCTATCAGA CACTCAACTC AAAGTATTCT 
AAAGGTCTTC ATGTGGAGAC GCTGGAGACT GAGAAGAAGG AGCGTTTCAT CCAGATCAGT 
AAAGTGGATG ATGAGGAGAG GAGGAGGAGA GAGCAGCAGA AACAGCAGAG AGAGCAGGAG 
GAGCTGAATG ATGCTGTGGG TTTCTCCAGG GTTATCAGAG CCATCTCCAA ATCTGGTAAG 
CTGGTGGTGG GCCACAACAT GCTGCTGGAC GTCATGCACA CCATACACCA GTTCTGCGGG 
CCGCTGCCGG AGGAGCTGGA TGACTTCAAG GAGGTGGCCA TGACTGTGTT TCCCAGGCTG 
CTAGACACCA AGCTGATGGC GTCCACACAA CCGTTCAAGG AGATCATCCA CAACACCTCA 
CTGGCTGAAC TACACAAACA GCTGAGACAG AAACCCTTCA GACCACCCAC CACTGAGTGT 
CCTGAAGGCC TCCAGAGTTA CGACACCTCC ACCGAGCAGC TTCATGAAGC TGGATATGAC 
GCCTTCATCA CCGGACTCTG CTTCATCTCC ATGGCCAACT ACCTGGGCTC TTTCCTCACG 
CCGCCCAAGA GCCACATCTC TGCCCGCTCC AAACTGCTGG AGCCCTTCTA CAACAAATTA 
TTCCTGATGA GGGTCATCGA CATTCCCTAC CTGAACATGA GCGGACCTGA CTTGCAGCCC 
AAGAGAGATC ATGTCCTGTA CGTCACGTTC CCTAAAGAGT GGAAGACCAG TGACCTGTAC 
CAGCTGTTCA GCGCCTTCGG GAACATCCAG GTGTCGTGGG TGGACGACAC GTCAGCGTTC 
GTGTCTCTCA GTCAGACAGA GCAGGTTCAG ATCGCCATGA ACACCAGCCG ATACGCTGAG 
AGCTACCGCA TCCAGACCTA TGCGGAGTAC CTGCAGTCCC GGCAGAAGAA CACACACTCC 
AGCAGGAAGT GGGCCAGCGA TGGGTGGGCG GATACTTCCT ACCCCAGTGT CGCCATGACA 
ACCGCATCCG GCTACAGCCA CACCGACAAC CGGCATCAGG CGGTCAAGCG CAGCATCAGT 
CCGTCTCTGG ATGAGCAGAA TCACGGCGCC GACAGCAGCT GGACGAACTA CAGCGTCAAG 
AAGATCAAGA CTGAGGGAAG CTGCACGCAG ACCTACGCTG ACGTCGCCGG CTCCTGTGAT 
TGGCCTAGAC TGCAGGCTGA TGAAGGCGGA GCCTCTGTGA GCCCAGTGGC AGAGGAGGCG 
GAGCTCGATG AGTTCTCAGC CAATCAGAGT CAAGGTAAAA GGAGCCGCAA GCACAAGAAG 
AGGAAGTCAG ACGCTTCTGA AACTACACCG CCAGCGCTCT TTGACGTCCC TCAGGTTTGG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001307333.1
CDS	76..2058
Translation	MEVTRQNFKEILPEVCNAVQEADFISIDGEFTGISDGPSVSALTNGLDTPEERYTKLRKHSMNFLLFQFGVCTFRYDQNQSTYITKAFNFYIFPKPFSRTSPDIKFICQSSSIDFLASQGFDFNKVFRSGIPYLTQEEECQLREQYEERRGQMNGAGPVSYTPPSGTGVCNVPEDQREFIRSVEEKVEALLKNTDQTLDLEPCTGFQRKLIYQTLNSKYSKGLHVETLETEKKERFIQISKVDDEERRRREQQKQQREQEELNDAVGFSRVIRAISKSGKLVVGHNMLLDVMHTIHQFCGPLPEELDDFKEVAMTVFPRLLDTKLMASTQPFKEIIHNTSLAELHKQLRQKPFRPPTTECPEGLQSYDTSTEQLHEAGYDAFITGLCFISMANYLGSFLTPPKSHISARSKLLEPFYNKLFLMRVIDIPYLNMSGPDLQPKRDHVLYVTFPKEWKTSDLYQLFSAFGNIQVSWVDDTSAFVSLSQTEQVQIAMNTSRYAESYRIQTYAEYLQSRQKNTHSSRKWASDGWADTSYPSVAMTTASGYSHTDNRHQAVKRSISPSLDEQNHGADSSWTNYSVKKIKTEGSCTQTYADVAGSCDWPRLQADEGGASVSPVAEEAELDEFSANQSQGKRSRKHKKRKSDASETTPPALFDVPQVW

Target ORF information:

RefSeq Version	NM_001320404.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio poly(A)-specific ribonuclease (deadenylation nuclease) (parn), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001320404.1

ORF Insert Sequence:

ATGGAGGTCA CGAGACAGAA TTTTAAGGAG ATTCTTCCAG AGGTGTGTAA CGCGGTGCAG 
GAGGCGGATT TCATCTCTAT CGACGGAGAG TTCACAGGAA TCAGTGATGG GCCGTCTGTC 
AGTGCTCTGA CCAATGGACT GGACACTCCA GAGGAGAGAT ACACCAAACT GAGGAAGCAC 
TCCATGAACT TCCTGCTGTT CCAGTTCGGC GTCTGCACCT TCAGATACGA CCAGAACCAG 
TCGACGTACA TCACTAAAGC CTTCAACTTC TACATCTTCC CCAAACCCTT CAGCCGCACG 
TCTCCAGATA TAAAGTTCAT CTGTCAGAGC TCCAGTATTG ACTTTCTGGC CAGTCAGGGC 
TTCGACTTCA ACAAGGTCTT CCGCAGCGGG ATCCCATACC TAACTCAGGA GGAGGAGTGT 
CAGCTGAGGG AGCAGTATGA GGAGAGGCGG GGTCAGATGA ATGGGGCGGG CCCAGTCTCA 
TACACGCCCC CTTCAGGCAC TGGTGTGTGT AACGTGCCAG AGGACCAGAG AGAGTTCATC 
AGGAGTGTGG AGGAAAAGGT TGAAGCTCTA CTAAAGAACA CTGATCAGAC CCTGGATCTG 
GAGCCCTGCA CCGGGTTTCA GAGGAAGCTC ATCTATCAGA CACTCAACTC AAAGTATTCT 
AAAGGTCTTC ATGTGGAGAC GCTGGAGACT GAGAAGAAGG AGCGTTTCAT CCAGATCAGT 
AAAGTGGATG ATGAGGAGAG GAGGAGGAGA GAGCAGCAGA AACAGCAGAG AGAGCAGGAG 
GAGCTGAATG ATGCTGTGGG TTTCTCCAGG GTTATCAGAG CCATCTCCAA ATCTGGTAAG 
CTGGTGGTGG GCCACAACAT GCTGCTGGAC GTCATGCACA CCATACACCA GTTCTGCGGG 
CCGCTGCCGG AGGAGCTGGA TGACTTCAAG GAGGTGGCCA TGACTGTGTT TCCCAGGCTG 
CTAGACACCA AGCTGATGGC GTCCACACAA CCGTTCAAGG AGATCATCCA CAACACCTCA 
CTGGCTGAAC TACACAAACA GCTGAGACAG AAACCCTTCA GACCACCCAC CACTGAGTGT 
CCTGAAGGCC TCCAGAGTTA CGACACCTCC ACCGAGCAGC TTCATGAAGC TGGATATGAC 
GCCTTCATCA CCGGACTCTG CTTCATCTCC ATGGCCAACT ACCTGGGCTC TTTCCTCACG 
CCGCCCAAGA GCCACATCTC TGCCCGCTCC AAACTGCTGG AGCCCTTCTA CAACAAATTA 
TTCCTGATGA GGGTCATCGA CATTCCCTAC CTGAACATGA GCGGACCTGA CTTGCAGCCC 
AAGAGAGATC ATGTCCTGTA CGTCACGTTC CCTAAAGAGT GGAAGACCAG TGACCTGTAC 
CAGCTGTTCA GCGCCTTCGG GAACATCCAG GTGTCGTGGG TGGACGACAC GTCAGCGTTC 
GTGTCTCTCA GTCAGACAGA GCAGGTTCAG ATCGCCATGA ACACCAGCCG ATACGCTGAG 
AGCTACCGCA TCCAGACCTA TGCGGAGTAC CTGCAGTCCC GGCAGAAGAA CACACACTCC 
AGCAGGAAGT GGGCCAGCGA TGGGTGGGCG GATACTTCCT ACCCCAGTGT CGCCATGACA 
ACCGCATCCG GCTACAGCCA CACCGACAAC CGGCATCAGG CGGTCAAGCG CAGCATCAGT 
CCGTCTCTGG ATGAGCAGAA TCACGGCGCC GACAGCAGCT GGACGAACTA CAGCGTCAAG 
AAGATCAAGA CTGAGGGAAG CTGCACGCAG ACCTACGCTG ACGTCGCCGG CTCCTGTGAT 
TGGCCTAGAC TGCAGGCTGA TGAAGGCGGA GCCTCTGTGA GCCCAGTGGC AGAGGAGGCG 
GAGCTCGATG AGTTCTCAGC CAATCAGAGT CAAGGTAAAA GGAGCCGCAA GCACAAGAAG 
AGGAAGTCAG ACGCTTCTGA AACTACACCG CCAGCGCTCT TTGACGTCCC TCAGGTTTGG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Protein palmitoylation activate zygotic gene expression during the maternal-to-zygotic transition.
Biochemical and biophysical research communications475(2)194-201(2016 06)
Du Z,Chen X,Li X,He K,Ji S,Shi W,Hao A

RNA structure replaces the need for U2AF2 in splicing.
Genome research26(1)12-23(2016 Jan)
Lin CL,Taggart AJ,Lim KH,Cygan KJ,Ferraris L,Creton R,Huang YT,Fairbrother WG

Bone marrow failure and developmental delay caused by mutations in poly(A)-specific ribonuclease (PARN).
Journal of medical genetics52(11)738-48(2015 Nov)
Dhanraj S,Gunja SM,Deveau AP,Nissbeck M,Boonyawat B,Coombs AJ,Renieri A,Mucciolo M,Marozza A,Buoni S,Turner L,Li H,Jarrar A,Sabanayagam M,Kirby M,Shago M,Pinto D,Berman JN,Scherer SW,Virtanen A,Dror Y

Online automated in vivo zebrafish phosphoproteomics: from large-scale analysis down to a single embryo.
Journal of proteome research7(4)1555-64(2008 Apr)
Lemeer S,Pinkse MW,Mohammed S,van Breukelen B,den Hertog J,Slijper M,Heck AJ

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parn cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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