astn1 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	astn1
Entrez Gene ID	791157
Full Name	astrotactin 1
Synonyms	zgc:113405,zgc:158703
General protein information	Preferred Names astrotactin 1 Names astrotactin-1
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_003197882.5	XP_003197930.4	astrotactin-1 isoform X1
XM_021467632.1	XP_021323307.1	astrotactin-1 isoform X2
XM_017351899.2	XP_017207388.1	astrotactin-1 isoform X4
XM_017351899.1	XP_017207388.1	astrotactin-1 isoform X3
XM_003197882.4	XP_003197930.4	astrotactin-1 isoform X1
XM_017351897.1	XP_017207386.1	astrotactin-1 isoform X2
XM_017351897.2	XP_017207386.1	astrotactin-1 isoform X3

Pathways from BioSystems

Homologies

Homo sapiens (human)	ASTN1	NP_004310.1
Canis lupus familiaris (dog)	ASTN1	XP_005622546.1
Bos taurus (cattle)	ASTN1	XP_002694147.2
Pan troglodytes (chimpanzee)	ASTN1	XP_514021.3
	ASTN1	XP_001105186.1
Xenopus tropicalis (tropical clawed frog)	astn1	NP_001072522.1
Mus musculus (house mouse)	Astn1	NP_031521.2
Rattus norvegicus (Norway rat)	Astn1	NP_001164074.1
Gallus gallus (chicken)	ASTN1	XP_004943315.1
Danio rerio (zebrafish)	astn1	XP_003197930.2

Gene Ontology

The following astn1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the astn1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa150474	XM_021467632.1 Latest version!	Danio rerio astrotactin 1 (astn1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
ODa56453	XM_003197882.5 Latest version!	Danio rerio astrotactin 1 (astn1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
ODa56454	XM_017351897.2 Latest version!	Danio rerio astrotactin 1 (astn1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
ODa56455	XM_017351899.2 Latest version!	Danio rerio astrotactin 1 (astn1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
View Old Accession Versions >>
ODa56455	XM_017351899.1	Danio rerio astrotactin 1 (astn1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
ODa56453	XM_003197882.4	Danio rerio astrotactin 1 (astn1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
ODa56454	XM_017351897.1	Danio rerio astrotactin 1 (astn1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

astn1 ( XM_021467632.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021323307.1 Danio rerio astrotactin 1 (astn1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa150474
Clone ID Related Accession (Same CDS sequence)	XM_021467632.1
Accession Version	XM_021467632.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3930bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	astrotactin-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGCTAC CGCCGCGCTC CTGTATGATG CTACCGCCGC GCTCCTATCT GCTTATCGGC 
GTTTTTTGGA TTGTCCTCTT CAGCTCTGCT GGCGTAAAAT GTCTGAACGG CGAAGAAGAT 
CAGTCTCAAG ACATGGAGTG CAAGATGAAA AGCGTTACGG TCTCCGCGTT GCCGTTTTTG 
CGAGAGAACG ACCTGAGCAT CATGCATAGC CCGTCGGCCT CCGAGCCCAA GCTACTCTTC 
TCCGTCAGGA ATGATTTTCC CGGCGAGATA GTCGTTGTTG ACGACTTGGA AAATACCGAG 
CTCCCGTACT TTGTGTTAGA GATCTCCGGG AACTCTGAAG ACATTCCTCT GGTGCGCTGG 
CGACAGCAGT GGTTGGAAAA TGGCACTTTG CTTTTCCACA TTCACCATCA GGACGGCAGT 
CAGAACTTGC CTGGTCCGGC GGCCACTGCT TACCCTGCTA GTGACTCTGC TGAAGAGGAG 
CTGCGCATTC TGCACATTTC TGTCATGGGT GGCATGATCG CTCTTCTGCT GTCCATCCTG 
TGCTTGGTGT TGATCCTGTA CACTCGCAGA CGCTGGTGTA AACGTCGCCG GGTTCCTCAA 
CCTCAAAAGA GCGCCAGTGC TGAAGCTGCC AATGAGATCC ACTACATCCC CTCAGTACTG 
ATGGGTGGAC AGGCCCGGGA AAGTCTGAGA AATTCGCGCG GGCAGGGCCA CAACTCGAGT 
GGCACCCTCA GCATCCGAGA GACCCCCATA CTGGATGGGT ACGAGTATGA CATCACTGAC 
CTGCGGCATC ACCTGCAGCG GGAGTGTATG AATGGTGGAG AGGACTTCAC CAGTCAGGTC 
ACCCGCACGC TAGACTCACT TCAGGGGTGC AATGAAAAGG GGGGCATGGA CCTCACTCCA 
GGGAGTGATA GCACAAAACT GTCCCTAATG AGCAAGTACA AGGATAACAT CATTGCTACC 
AGTCCTGTCG ACAGCAACCA CCAGCAAAAC ACACTTCTTC CACACAATAC CTCCACCAGC 
CAGCGCAAAA GGCTGTCCAG CAAAACACAC GCGGGTTCAA CTTTTCTGAA CCCAGATGGA 
GACTCTGGGA CAGAGGCGGA CAGCGAGCCT CAGCTGACTT TCTACACCGA CCCAAACCGC 
AGCCGCCGAC GCAGTCGAGG TATGAGAAAT GGTTCCCCAC GGAGTCCTAT CAATAAGACC 
ACCCTCACTT TGATCAGCGT GGTGAGCTGT GTGATCGGCC TGGTCTGTGC GTCTCACCTT 
TCCTGTAGCC TGAACGTCAG AGTGATCCTT CACGTACCGG AGCATCTCAT TGCAGACGGC 
TCCAGGTTTG TTCTGCTTCA GGGCAGTCAG CTGGATGCCT CTGATTGGCT GAACCCTGCC 
CAGGTGCTGC TATACTACCA GCAGAATGCC AGCGGCCCCT GGGTCAACGA TCTGTGTGGC 
CGGCGTCTCC TGGACCCCTG TGAGCACCAG TGTGACCCAG ATACAGGAGA ATGTCTCTGC 
TTTGATGGCT ACATGAAAGA CCCAGTTCAC AAACACCTTT GCATCCGCAA TGAATGGGGC 
CCAAATCAAG GCCCATGGCC CTACACAATT TTCCAACGGG GCTTTGATTT GGTGATGGGA 
GAGCAGCCAT CGGATCGCAT TTTTAGATTT ACATACACTC TTGGAGAAGG CATGTGGCTG 
CCACTTAGCA AAAGCTTCGT CATTCCTCCG GCTGAGCTGG CCATCAACCC CTCTGCCAAG 
TGTAAGACAG ATATGACTGT CATGGAGGAC GCAGTGGATG TCAGGGAGGA ACTGATGATT 
TCCTCATCAT TTGACAGTCT GGAGGTGCTG CTGGACTCAT TTGGCCCTGT GAGAGACTGT 
TCAAAAGACA ACGGAGGTTG CAGCCGAAAT TTCCGCTGTA TTTCTGATCG CAAGCTTGAC 
TCCACTGGCT GTGTGTGTCC TCCTGGATTG AGCCCGATGA AGGATGGCAC CGGTTGCTAT 
GACCACCACA TTGGCATTGA CTGCTCGGAC GGCTTTAATG GGGGCTGTGA GCAGCTGTGC 
CTCCAGCAGC TGGCACCTCT GGAGGAGGAT CCCACTCTCT ACAACATCCA GATGTTCTGC 
GGATGCATTG AAGATTATAA GAGAGGGTCA GATGGCCGCT CTTGTCAGCC ACTGTCTGAG 
GCCTGCACAG AGGGACTGGA CTGCGGGGAC ACAGCCGACA TCCCAGCCAA CCAGACTGTG 
TTTGGAGACC TCTTTTATGG CTATAATAAC CACACCAAAG AGATCACATC TGGACAGATA 
CTAAAAGCCA CTTTTAGGCA GAAAAACTTT GCTCGTGGCA TTGAGCAGCA GCTCCCGGAT 
GGCATGGTGG TGGCGTCAGT GCCGAATGAA GCCCAGTGTC ATGAGGAACT GTCTGACCCA 
GTGCCTGATC CAGAATACCT CACTGGTATG GTAAACTACA GTGAGGTTAC CGGTTACCCA 
TTGGTTCAGC ACTGGAGGCT GCGCTCTGTT ATGTACCATG TAAAGCTCAA TCAGTGGGCT 
CTTTCCCAAG CCTTCAGTTC TGCCATTCAT TCACTGGACG GGGCCACCCT GCGCAGCGAC 
TTTGTGTCCA TTTTAAAAGA GTTTGGCAAC CATTTTATCC AGGAGGCAGT GTATGGTTTT 
GAGGAGTCCT GTACCATATG GTATCCCAAC AAACAAGTCC AACGCCAGCT GTGGTTGGAG 
TACCAGGATA TCAGCAAAGG AAACTCTCCA TCGGACGAGT CGGAGGAGCG AGACAAAGAG 
CCACGCACAC TTACTTACCC AGCATACATT GCCAGCCTGC TGGACTCTGG TGCCAAGCGC 
ATGGCGGCGG GGGTGCGCAT GGACTGCAGC AGCCAGGGCC AGTGCCCTCT GTCCTGCCAC 
CTCTGCCACA TGAGCCCTGG GCCTGCACGT CCTGCAGAAC CTGTGCTGCT TAACATCACT 
AAAGCCACAC CGATCTTCGA ACTTGTCAAC AGCAACGAGA CGTACCAGGC TCTGCAGGAG 
GCCATGATGA GTGTTTTATG GTGCTCTGCT AAAGGGGATG TTATTGATGA CTGGTGCAGA 
TGTGACTCCA ATGCTTTTGG CACAGATGGA CTGCCAACCT GTGCTCCGCT GCCTCAACCT 
ATGCTCAAAC TGTCCCATTC GTATGAGCCC AGCAGCTCAT TGGTCATCAT AGAATGGAAT 
CACACAGAGC CACCAATTGG AGTGAGGATT GCTGATTACC TCATCAGTCA GGAGAAGGTG 
ACAGAGCGGA CTGATCACTC CAAAGTTGAG ACGGAGACAA TGTTGAGTTT TGTGGATGAC 
ATCATGTCAG GGGCCAAATC CCCGTGTGTG ATGCTTGGAG ACATTCCCGA TCCTCTGTCT 
TCAATCTCCT TGATCATTCG CTGCCTGGAG CCGGACACCA CCTACATGTT TCGGCTGTGG 
GCAGTGGACA ACACGGGTCG GCGCTCCAGT CCGAGTGAGG TGACGATCAA AACTCCTTGT 
CCTGCTGTGG ATGATGTCAA GGCTCAAGAA ATAGCAGATA AGATTTACAA TCTGTTCAAC 
GGCTACACCA GCGGGAAGGA GCAGCAGACG GCCTATAATA CACTCATGGA TCTGGGATCA 
CCAACCCTCC ACCGAGTTCT ATACCACTAC AATCAGCGCT ACGAGAGTTT TGGAGAGTTC 
ACCTGGCGAT GTGAAGATGA ACTTGGTCCA AGGAAGGCAG GACTGATCCT GTCTCAGCTG 
GATGAGCTCA GCGGTTGGTG TCGGGGGCTC CTCCAGGAAC CAAAGATTGG CCTGCGCAGA 
GCATCCCTCA AGTATCTGTC TTGTCGATAC ACAGATACCA AAGCGTTCGG GCTCAGCTGG 
GTGAACCTCG GCCAAGACTT GCGTAAGGCC TGCGAGGAGC AGATGCTGTC CGTGATGTAC 
AACGATTATG GAGAACCCAA GGAACTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021323307.1
CDS	329..4258
Translation	MMLPPRSCMMLPPRSYLLIGVFWIVLFSSAGVKCLNGEEDQSQDMECKMKSVTVSALPFLRENDLSIMHSPSASEPKLLFSVRNDFPGEIVVVDDLENTELPYFVLEISGNSEDIPLVRWRQQWLENGTLLFHIHHQDGSQNLPGPAATAYPASDSAEEELRILHISVMGGMIALLLSILCLVLILYTRRRWCKRRRVPQPQKSASAEAANEIHYIPSVLMGGQARESLRNSRGQGHNSSGTLSIRETPILDGYEYDITDLRHHLQRECMNGGEDFTSQVTRTLDSLQGCNEKGGMDLTPGSDSTKLSLMSKYKDNIIATSPVDSNHQQNTLLPHNTSTSQRKRLSSKTHAGSTFLNPDGDSGTEADSEPQLTFYTDPNRSRRRSRGMRNGSPRSPINKTTLTLISVVSCVIGLVCASHLSCSLNVRVILHVPEHLIADGSRFVLLQGSQLDASDWLNPAQVLLYYQQNASGPWVNDLCGRRLLDPCEHQCDPDTGECLCFDGYMKDPVHKHLCIRNEWGPNQGPWPYTIFQRGFDLVMGEQPSDRIFRFTYTLGEGMWLPLSKSFVIPPAELAINPSAKCKTDMTVMEDAVDVREELMISSSFDSLEVLLDSFGPVRDCSKDNGGCSRNFRCISDRKLDSTGCVCPPGLSPMKDGTGCYDHHIGIDCSDGFNGGCEQLCLQQLAPLEEDPTLYNIQMFCGCIEDYKRGSDGRSCQPLSEACTEGLDCGDTADIPANQTVFGDLFYGYNNHTKEITSGQILKATFRQKNFARGIEQQLPDGMVVASVPNEAQCHEELSDPVPDPEYLTGMVNYSEVTGYPLVQHWRLRSVMYHVKLNQWALSQAFSSAIHSLDGATLRSDFVSILKEFGNHFIQEAVYGFEESCTIWYPNKQVQRQLWLEYQDISKGNSPSDESEERDKEPRTLTYPAYIASLLDSGAKRMAAGVRMDCSSQGQCPLSCHLCHMSPGPARPAEPVLLNITKATPIFELVNSNETYQALQEAMMSVLWCSAKGDVIDDWCRCDSNAFGTDGLPTCAPLPQPMLKLSHSYEPSSSLVIIEWNHTEPPIGVRIADYLISQEKVTERTDHSKVETETMLSFVDDIMSGAKSPCVMLGDIPDPLSSISLIIRCLEPDTTYMFRLWAVDNTGRRSSPSEVTIKTPCPAVDDVKAQEIADKIYNLFNGYTSGKEQQTAYNTLMDLGSPTLHRVLYHYNQRYESFGEFTWRCEDELGPRKAGLILSQLDELSGWCRGLLQEPKIGLRRASLKYLSCRYTDTKAFGLSWVNLGQDLRKACEEQMLSVMYNDYGEPKEL

Target ORF information:

RefSeq Version	XM_021467632.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio astrotactin 1 (astn1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021467632.1

ORF Insert Sequence:

ATGATGCTAC CGCCGCGCTC CTGTATGATG CTACCGCCGC GCTCCTATCT GCTTATCGGC 
GTTTTTTGGA TTGTCCTCTT CAGCTCTGCT GGCGTAAAAT GTCTGAACGG CGAAGAAGAT 
CAGTCTCAAG ACATGGAGTG CAAGATGAAA AGCGTTACGG TCTCCGCGTT GCCGTTTTTG 
CGAGAGAACG ACCTGAGCAT CATGCATAGC CCGTCGGCCT CCGAGCCCAA GCTACTCTTC 
TCCGTCAGGA ATGATTTTCC CGGCGAGATA GTCGTTGTTG ACGACTTGGA AAATACCGAG 
CTCCCGTACT TTGTGTTAGA GATCTCCGGG AACTCTGAAG ACATTCCTCT GGTGCGCTGG 
CGACAGCAGT GGTTGGAAAA TGGCACTTTG CTTTTCCACA TTCACCATCA GGACGGCAGT 
CAGAACTTGC CTGGTCCGGC GGCCACTGCT TACCCTGCTA GTGACTCTGC TGAAGAGGAG 
CTGCGCATTC TGCACATTTC TGTCATGGGT GGCATGATCG CTCTTCTGCT GTCCATCCTG 
TGCTTGGTGT TGATCCTGTA CACTCGCAGA CGCTGGTGTA AACGTCGCCG GGTTCCTCAA 
CCTCAAAAGA GCGCCAGTGC TGAAGCTGCC AATGAGATCC ACTACATCCC CTCAGTACTG 
ATGGGTGGAC AGGCCCGGGA AAGTCTGAGA AATTCGCGCG GGCAGGGCCA CAACTCGAGT 
GGCACCCTCA GCATCCGAGA GACCCCCATA CTGGATGGGT ACGAGTATGA CATCACTGAC 
CTGCGGCATC ACCTGCAGCG GGAGTGTATG AATGGTGGAG AGGACTTCAC CAGTCAGGTC 
ACCCGCACGC TAGACTCACT TCAGGGGTGC AATGAAAAGG GGGGCATGGA CCTCACTCCA 
GGGAGTGATA GCACAAAACT GTCCCTAATG AGCAAGTACA AGGATAACAT CATTGCTACC 
AGTCCTGTCG ACAGCAACCA CCAGCAAAAC ACACTTCTTC CACACAATAC CTCCACCAGC 
CAGCGCAAAA GGCTGTCCAG CAAAACACAC GCGGGTTCAA CTTTTCTGAA CCCAGATGGA 
GACTCTGGGA CAGAGGCGGA CAGCGAGCCT CAGCTGACTT TCTACACCGA CCCAAACCGC 
AGCCGCCGAC GCAGTCGAGG TATGAGAAAT GGTTCCCCAC GGAGTCCTAT CAATAAGACC 
ACCCTCACTT TGATCAGCGT GGTGAGCTGT GTGATCGGCC TGGTCTGTGC GTCTCACCTT 
TCCTGTAGCC TGAACGTCAG AGTGATCCTT CACGTACCGG AGCATCTCAT TGCAGACGGC 
TCCAGGTTTG TTCTGCTTCA GGGCAGTCAG CTGGATGCCT CTGATTGGCT GAACCCTGCC 
CAGGTGCTGC TATACTACCA GCAGAATGCC AGCGGCCCCT GGGTCAACGA TCTGTGTGGC 
CGGCGTCTCC TGGACCCCTG TGAGCACCAG TGTGACCCAG ATACAGGAGA ATGTCTCTGC 
TTTGATGGCT ACATGAAAGA CCCAGTTCAC AAACACCTTT GCATCCGCAA TGAATGGGGC 
CCAAATCAAG GCCCATGGCC CTACACAATT TTCCAACGGG GCTTTGATTT GGTGATGGGA 
GAGCAGCCAT CGGATCGCAT TTTTAGATTT ACATACACTC TTGGAGAAGG CATGTGGCTG 
CCACTTAGCA AAAGCTTCGT CATTCCTCCG GCTGAGCTGG CCATCAACCC CTCTGCCAAG 
TGTAAGACAG ATATGACTGT CATGGAGGAC GCAGTGGATG TCAGGGAGGA ACTGATGATT 
TCCTCATCAT TTGACAGTCT GGAGGTGCTG CTGGACTCAT TTGGCCCTGT GAGAGACTGT 
TCAAAAGACA ACGGAGGTTG CAGCCGAAAT TTCCGCTGTA TTTCTGATCG CAAGCTTGAC 
TCCACTGGCT GTGTGTGTCC TCCTGGATTG AGCCCGATGA AGGATGGCAC CGGTTGCTAT 
GACCACCACA TTGGCATTGA CTGCTCGGAC GGCTTTAATG GGGGCTGTGA GCAGCTGTGC 
CTCCAGCAGC TGGCACCTCT GGAGGAGGAT CCCACTCTCT ACAACATCCA GATGTTCTGC 
GGATGCATTG AAGATTATAA GAGAGGGTCA GATGGCCGCT CTTGTCAGCC ACTGTCTGAG 
GCCTGCACAG AGGGACTGGA CTGCGGGGAC ACAGCCGACA TCCCAGCCAA CCAGACTGTG 
TTTGGAGACC TCTTTTATGG CTATAATAAC CACACCAAAG AGATCACATC TGGACAGATA 
CTAAAAGCCA CTTTTAGGCA GAAAAACTTT GCTCGTGGCA TTGAGCAGCA GCTCCCGGAT 
GGCATGGTGG TGGCGTCAGT GCCGAATGAA GCCCAGTGTC ATGAGGAACT GTCTGACCCA 
GTGCCTGATC CAGAATACCT CACTGGTATG GTAAACTACA GTGAGGTTAC CGGTTACCCA 
TTGGTTCAGC ACTGGAGGCT GCGCTCTGTT ATGTACCATG TAAAGCTCAA TCAGTGGGCT 
CTTTCCCAAG CCTTCAGTTC TGCCATTCAT TCACTGGACG GGGCCACCCT GCGCAGCGAC 
TTTGTGTCCA TTTTAAAAGA GTTTGGCAAC CATTTTATCC AGGAGGCAGT GTATGGTTTT 
GAGGAGTCCT GTACCATATG GTATCCCAAC AAACAAGTCC AACGCCAGCT GTGGTTGGAG 
TACCAGGATA TCAGCAAAGG AAACTCTCCA TCGGACGAGT CGGAGGAGCG AGACAAAGAG 
CCACGCACAC TTACTTACCC AGCATACATT GCCAGCCTGC TGGACTCTGG TGCCAAGCGC 
ATGGCGGCGG GGGTGCGCAT GGACTGCAGC AGCCAGGGCC AGTGCCCTCT GTCCTGCCAC 
CTCTGCCACA TGAGCCCTGG GCCTGCACGT CCTGCAGAAC CTGTGCTGCT TAACATCACT 
AAAGCCACAC CGATCTTCGA ACTTGTCAAC AGCAACGAGA CGTACCAGGC TCTGCAGGAG 
GCCATGATGA GTGTTTTATG GTGCTCTGCT AAAGGGGATG TTATTGATGA CTGGTGCAGA 
TGTGACTCCA ATGCTTTTGG CACAGATGGA CTGCCAACCT GTGCTCCGCT GCCTCAACCT 
ATGCTCAAAC TGTCCCATTC GTATGAGCCC AGCAGCTCAT TGGTCATCAT AGAATGGAAT 
CACACAGAGC CACCAATTGG AGTGAGGATT GCTGATTACC TCATCAGTCA GGAGAAGGTG 
ACAGAGCGGA CTGATCACTC CAAAGTTGAG ACGGAGACAA TGTTGAGTTT TGTGGATGAC 
ATCATGTCAG GGGCCAAATC CCCGTGTGTG ATGCTTGGAG ACATTCCCGA TCCTCTGTCT 
TCAATCTCCT TGATCATTCG CTGCCTGGAG CCGGACACCA CCTACATGTT TCGGCTGTGG 
GCAGTGGACA ACACGGGTCG GCGCTCCAGT CCGAGTGAGG TGACGATCAA AACTCCTTGT 
CCTGCTGTGG ATGATGTCAA GGCTCAAGAA ATAGCAGATA AGATTTACAA TCTGTTCAAC 
GGCTACACCA GCGGGAAGGA GCAGCAGACG GCCTATAATA CACTCATGGA TCTGGGATCA 
CCAACCCTCC ACCGAGTTCT ATACCACTAC AATCAGCGCT ACGAGAGTTT TGGAGAGTTC 
ACCTGGCGAT GTGAAGATGA ACTTGGTCCA AGGAAGGCAG GACTGATCCT GTCTCAGCTG 
GATGAGCTCA GCGGTTGGTG TCGGGGGCTC CTCCAGGAAC CAAAGATTGG CCTGCGCAGA 
GCATCCCTCA AGTATCTGTC TTGTCGATAC ACAGATACCA AAGCGTTCGG GCTCAGCTGG 
GTGAACCTCG GCCAAGACTT GCGTAAGGCC TGCGAGGAGC AGATGCTGTC CGTGATGTAC 
AACGATTATG GAGAACCCAA GGAACTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

astn1 ( XM_003197882.5 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_003197930.4 Danio rerio astrotactin 1 (astn1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa56453
Clone ID Related Accession (Same CDS sequence)	XM_003197882.5 , XM_003197882.4
Accession Version	XM_003197882.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3960bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	astrotactin-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_003197882.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGCTAC CGCCGCGCTC CTGTATGATG CTACCGCCGC GCTCCTATCT GCTTATCGGC 
GTTTTTTGGA TTGTCCTCTT CAGCTCTGCT GGCGTAAAAT GTCTGAACGG CGAAGAAGAT 
CAGTCTCAAG ACATGGAGTG CAAGATGAAA AGCGTTACGG TCTCCGCGTT GCCGTTTTTG 
CGAGAGAACG ACCTGAGCAT CATGCATAGC CCGTCGGCCT CCGAGCCCAA GCTACTCTTC 
TCCGTCAGGA ATGATTTTCC CGGCGAGATA GTCGTTGTTG ACGACTTGGA AAATACCGAG 
CTCCCGTACT TTGTGTTAGA GATCTCCGGG AACTCTGAAG ACATTCCTCT GGTGCGCTGG 
CGACAGCAGT GGTTGGAAAA TGGCACTTTG CTTTTCCACA TTCACCATCA GGACGGCAGT 
CAGAACTTGC CTGGTCCGGC GGCCACTGCT TACCCTGCTA GTGACTCTGC TGAAGAGGAG 
CTGCGCATTC TGCACATTTC TGTCATGGGT GGCATGATCG CTCTTCTGCT GTCCATCCTG 
TGCTTGGTGT TGATCCTGTA CACTCGCAGA CGCTGGTGTA AACGTCGCCG GGTTCCTCAA 
CCTCAAAAGA GCGCCAGTGC TGAAGCTGCC AATGAGATCC ACTACATCCC CTCAGTACTG 
ATGGGTGGAC AGGCCCGGGA AAGTCTGAGA AATTCGCGCG GGCAGGGCCA CAACTCGAGT 
GGCACCCTCA GCATCCGAGA GACCCCCATA CTGGATGGGT ACGAGTATGA CATCACTGAC 
CTGCGGCATC ACCTGCAGCG GGAGTGTATG AATGGTGGAG AGGACTTCAC CAGTCAGGTC 
ACCCGCACGC TAGACTCACT TCAGGGGTGC AATGAAAAGG GGGGCATGGA CCTCACTCCA 
GGGAGTGATA GCACAAAACT GTCCCTAATG AGCAAGTACA AGGATAACAT CATTGCTACC 
AGTCCTGTCG ACAGCAACCA CCAGCAAAAC ACACTTCTTC CACACAATAC CTCCACCAGC 
CAGCGCAAAA GGCTGTCCAG CAAAACACAC GCGGGTTCAA CTTTTCTGAA CCCAGATGGA 
GACTCTGGGA CAGAGGCGGA CAGCGAGCCT CAGCTGACTT TCTACACCGA CCCAAACCGC 
AGCCGCCGAC GCAGTCGAGG TATGAGAAAT GGTAACGTCA ACTCGGCGTG GGGCTCTGGG 
GGTTCCCCAC GGAGTCCTAT CAATAAGACC ACCCTCACTT TGATCAGCGT GGTGAGCTGT 
GTGATCGGCC TGGTCTGTGC GTCTCACCTT TCCTGTAGCC TGAACGTCAG AGTGATCCTT 
CACGTACCGG AGCATCTCAT TGCAGACGGC TCCAGGTTTG TTCTGCTTCA GGGCAGTCAG 
CTGGATGCCT CTGATTGGCT GAACCCTGCC CAGGTGCTGC TATACTACCA GCAGAATGCC 
AGCGGCCCCT GGGTCAACGA TCTGTGTGGC CGGCGTCTCC TGGACCCCTG TGAGCACCAG 
TGTGACCCAG ATACAGGAGA ATGTCTCTGC TTTGATGGCT ACATGAAAGA CCCAGTTCAC 
AAACACCTTT GCATCCGCAA TGAATGGGGC CCAAATCAAG GCCCATGGCC CTACACAATT 
TTCCAACGGG GCTTTGATTT GGTGATGGGA GAGCAGCCAT CGGATCGCAT TTTTAGATTT 
ACATACACTC TTGGAGAAGG CATGTGGCTG CCACTTAGCA AAAGCTTCGT CATTCCTCCG 
GCTGAGCTGG CCATCAACCC CTCTGCCAAG TGTAAGACAG ATATGACTGT CATGGAGGAC 
GCAGTGGATG TCAGGGAGGA ACTGATGATT TCCTCATCAT TTGACAGTCT GGAGGTGCTG 
CTGGACTCAT TTGGCCCTGT GAGAGACTGT TCAAAAGACA ACGGAGGTTG CAGCCGAAAT 
TTCCGCTGTA TTTCTGATCG CAAGCTTGAC TCCACTGGCT GTGTGTGTCC TCCTGGATTG 
AGCCCGATGA AGGATGGCAC CGGTTGCTAT GACCACCACA TTGGCATTGA CTGCTCGGAC 
GGCTTTAATG GGGGCTGTGA GCAGCTGTGC CTCCAGCAGC TGGCACCTCT GGAGGAGGAT 
CCCACTCTCT ACAACATCCA GATGTTCTGC GGATGCATTG AAGATTATAA GAGAGGGTCA 
GATGGCCGCT CTTGTCAGCC ACTGTCTGAG GCCTGCACAG AGGGACTGGA CTGCGGGGAC 
ACAGCCGACA TCCCAGCCAA CCAGACTGTG TTTGGAGACC TCTTTTATGG CTATAATAAC 
CACACCAAAG AGATCACATC TGGACAGATA CTAAAAGCCA CTTTTAGGCA GAAAAACTTT 
GCTCGTGGCA TTGAGCAGCA GCTCCCGGAT GGCATGGTGG TGGCGTCAGT GCCGAATGAA 
GCCCAGTGTC ATGAGGAACT GTCTGACCCA GTGCCTGATC CAGAATACCT CACTGGTATG 
GTAAACTACA GTGAGGTTAC CGGTTACCCA TTGGTTCAGC ACTGGAGGCT GCGCTCTGTT 
ATGTACCATG TAAAGCTCAA TCAGTGGGCT CTTTCCCAAG CCTTCAGTTC TGCCATTCAT 
TCACTGGACG GGGCCACCCT GCGCAGCGAC TTTGTGTCCA TTTTAAAAGA GTTTGGCAAC 
CATTTTATCC AGGAGGCAGT GTATGGTTTT GAGGAGTCCT GTACCATATG GTATCCCAAC 
AAACAAGTCC AACGCCAGCT GTGGTTGGAG TACCAGGATA TCAGCAAAGG AAACTCTCCA 
TCGGACGAGT CGGAGGAGCG AGACAAAGAG CCACGCACAC TTACTTACCC AGCATACATT 
GCCAGCCTGC TGGACTCTGG TGCCAAGCGC ATGGCGGCGG GGGTGCGCAT GGACTGCAGC 
AGCCAGGGCC AGTGCCCTCT GTCCTGCCAC CTCTGCCACA TGAGCCCTGG GCCTGCACGT 
CCTGCAGAAC CTGTGCTGCT TAACATCACT AAAGCCACAC CGATCTTCGA ACTTGTCAAC 
AGCAACGAGA CGTACCAGGC TCTGCAGGAG GCCATGATGA GTGTTTTATG GTGCTCTGCT 
AAAGGGGATG TTATTGATGA CTGGTGCAGA TGTGACTCCA ATGCTTTTGG CACAGATGGA 
CTGCCAACCT GTGCTCCGCT GCCTCAACCT ATGCTCAAAC TGTCCCATTC GTATGAGCCC 
AGCAGCTCAT TGGTCATCAT AGAATGGAAT CACACAGAGC CACCAATTGG AGTGAGGATT 
GCTGATTACC TCATCAGTCA GGAGAAGGTG ACAGAGCGGA CTGATCACTC CAAAGTTGAG 
ACGGAGACAA TGTTGAGTTT TGTGGATGAC ATCATGTCAG GGGCCAAATC CCCGTGTGTG 
ATGCTTGGAG ACATTCCCGA TCCTCTGTCT TCAATCTCCT TGATCATTCG CTGCCTGGAG 
CCGGACACCA CCTACATGTT TCGGCTGTGG GCAGTGGACA ACACGGGTCG GCGCTCCAGT 
CCGAGTGAGG TGACGATCAA AACTCCTTGT CCTGCTGTGG ATGATGTCAA GGCTCAAGAA 
ATAGCAGATA AGATTTACAA TCTGTTCAAC GGCTACACCA GCGGGAAGGA GCAGCAGACG 
GCCTATAATA CACTCATGGA TCTGGGATCA CCAACCCTCC ACCGAGTTCT ATACCACTAC 
AATCAGCGCT ACGAGAGTTT TGGAGAGTTC ACCTGGCGAT GTGAAGATGA ACTTGGTCCA 
AGGAAGGCAG GACTGATCCT GTCTCAGCTG GATGAGCTCA GCGGTTGGTG TCGGGGGCTC 
CTCCAGGAAC CAAAGATTGG CCTGCGCAGA GCATCCCTCA AGTATCTGTC TTGTCGATAC 
ACAGATACCA AAGCGTTCGG GCTCAGCTGG GTGAACCTCG GCCAAGACTT GCGTAAGGCC 
TGCGAGGAGC AGATGCTGTC CGTGATGTAC AACGATTATG GAGAACCCAA GGAACTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003197930.4
CDS	328..4287
Translation	MMLPPRSCMMLPPRSYLLIGVFWIVLFSSAGVKCLNGEEDQSQDMECKMKSVTVSALPFLRENDLSIMHSPSASEPKLLFSVRNDFPGEIVVVDDLENTELPYFVLEISGNSEDIPLVRWRQQWLENGTLLFHIHHQDGSQNLPGPAATAYPASDSAEEELRILHISVMGGMIALLLSILCLVLILYTRRRWCKRRRVPQPQKSASAEAANEIHYIPSVLMGGQARESLRNSRGQGHNSSGTLSIRETPILDGYEYDITDLRHHLQRECMNGGEDFTSQVTRTLDSLQGCNEKGGMDLTPGSDSTKLSLMSKYKDNIIATSPVDSNHQQNTLLPHNTSTSQRKRLSSKTHAGSTFLNPDGDSGTEADSEPQLTFYTDPNRSRRRSRGMRNGNVNSAWGSGGSPRSPINKTTLTLISVVSCVIGLVCASHLSCSLNVRVILHVPEHLIADGSRFVLLQGSQLDASDWLNPAQVLLYYQQNASGPWVNDLCGRRLLDPCEHQCDPDTGECLCFDGYMKDPVHKHLCIRNEWGPNQGPWPYTIFQRGFDLVMGEQPSDRIFRFTYTLGEGMWLPLSKSFVIPPAELAINPSAKCKTDMTVMEDAVDVREELMISSSFDSLEVLLDSFGPVRDCSKDNGGCSRNFRCISDRKLDSTGCVCPPGLSPMKDGTGCYDHHIGIDCSDGFNGGCEQLCLQQLAPLEEDPTLYNIQMFCGCIEDYKRGSDGRSCQPLSEACTEGLDCGDTADIPANQTVFGDLFYGYNNHTKEITSGQILKATFRQKNFARGIEQQLPDGMVVASVPNEAQCHEELSDPVPDPEYLTGMVNYSEVTGYPLVQHWRLRSVMYHVKLNQWALSQAFSSAIHSLDGATLRSDFVSILKEFGNHFIQEAVYGFEESCTIWYPNKQVQRQLWLEYQDISKGNSPSDESEERDKEPRTLTYPAYIASLLDSGAKRMAAGVRMDCSSQGQCPLSCHLCHMSPGPARPAEPVLLNITKATPIFELVNSNETYQALQEAMMSVLWCSAKGDVIDDWCRCDSNAFGTDGLPTCAPLPQPMLKLSHSYEPSSSLVIIEWNHTEPPIGVRIADYLISQEKVTERTDHSKVETETMLSFVDDIMSGAKSPCVMLGDIPDPLSSISLIIRCLEPDTTYMFRLWAVDNTGRRSSPSEVTIKTPCPAVDDVKAQEIADKIYNLFNGYTSGKEQQTAYNTLMDLGSPTLHRVLYHYNQRYESFGEFTWRCEDELGPRKAGLILSQLDELSGWCRGLLQEPKIGLRRASLKYLSCRYTDTKAFGLSWVNLGQDLRKACEEQMLSVMYNDYGEPKEL

Target ORF information:

RefSeq Version	XM_003197882.5
Organism	Danio rerio(zebrafish)
Definition	Danio rerio astrotactin 1 (astn1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003197882.5

ORF Insert Sequence:

ATGATGCTAC CGCCGCGCTC CTGTATGATG CTACCGCCGC GCTCCTATCT GCTTATCGGC 
GTTTTTTGGA TTGTCCTCTT CAGCTCTGCT GGCGTAAAAT GTCTGAACGG CGAAGAAGAT 
CAGTCTCAAG ACATGGAGTG CAAGATGAAA AGCGTTACGG TCTCCGCGTT GCCGTTTTTG 
CGAGAGAACG ACCTGAGCAT CATGCATAGC CCGTCGGCCT CCGAGCCCAA GCTACTCTTC 
TCCGTCAGGA ATGATTTTCC CGGCGAGATA GTCGTTGTTG ACGACTTGGA AAATACCGAG 
CTCCCGTACT TTGTGTTAGA GATCTCCGGG AACTCTGAAG ACATTCCTCT GGTGCGCTGG 
CGACAGCAGT GGTTGGAAAA TGGCACTTTG CTTTTCCACA TTCACCATCA GGACGGCAGT 
CAGAACTTGC CTGGTCCGGC GGCCACTGCT TACCCTGCTA GTGACTCTGC TGAAGAGGAG 
CTGCGCATTC TGCACATTTC TGTCATGGGT GGCATGATCG CTCTTCTGCT GTCCATCCTG 
TGCTTGGTGT TGATCCTGTA CACTCGCAGA CGCTGGTGTA AACGTCGCCG GGTTCCTCAA 
CCTCAAAAGA GCGCCAGTGC TGAAGCTGCC AATGAGATCC ACTACATCCC CTCAGTACTG 
ATGGGTGGAC AGGCCCGGGA AAGTCTGAGA AATTCGCGCG GGCAGGGCCA CAACTCGAGT 
GGCACCCTCA GCATCCGAGA GACCCCCATA CTGGATGGGT ACGAGTATGA CATCACTGAC 
CTGCGGCATC ACCTGCAGCG GGAGTGTATG AATGGTGGAG AGGACTTCAC CAGTCAGGTC 
ACCCGCACGC TAGACTCACT TCAGGGGTGC AATGAAAAGG GGGGCATGGA CCTCACTCCA 
GGGAGTGATA GCACAAAACT GTCCCTAATG AGCAAGTACA AGGATAACAT CATTGCTACC 
AGTCCTGTCG ACAGCAACCA CCAGCAAAAC ACACTTCTTC CACACAATAC CTCCACCAGC 
CAGCGCAAAA GGCTGTCCAG CAAAACACAC GCGGGTTCAA CTTTTCTGAA CCCAGATGGA 
GACTCTGGGA CAGAGGCGGA CAGCGAGCCT CAGCTGACTT TCTACACCGA CCCAAACCGC 
AGCCGCCGAC GCAGTCGAGG TATGAGAAAT GGTAACGTCA ACTCGGCGTG GGGCTCTGGG 
GGTTCCCCAC GGAGTCCTAT CAATAAGACC ACCCTCACTT TGATCAGCGT GGTGAGCTGT 
GTGATCGGCC TGGTCTGTGC GTCTCACCTT TCCTGTAGCC TGAACGTCAG AGTGATCCTT 
CACGTACCGG AGCATCTCAT TGCAGACGGC TCCAGGTTTG TTCTGCTTCA GGGCAGTCAG 
CTGGATGCCT CTGATTGGCT GAACCCTGCC CAGGTGCTGC TATACTACCA GCAGAATGCC 
AGCGGCCCCT GGGTCAACGA TCTGTGTGGC CGGCGTCTCC TGGACCCCTG TGAGCACCAG 
TGTGACCCAG ATACAGGAGA ATGTCTCTGC TTTGATGGCT ACATGAAAGA CCCAGTTCAC 
AAACACCTTT GCATCCGCAA TGAATGGGGC CCAAATCAAG GCCCATGGCC CTACACAATT 
TTCCAACGGG GCTTTGATTT GGTGATGGGA GAGCAGCCAT CGGATCGCAT TTTTAGATTT 
ACATACACTC TTGGAGAAGG CATGTGGCTG CCACTTAGCA AAAGCTTCGT CATTCCTCCG 
GCTGAGCTGG CCATCAACCC CTCTGCCAAG TGTAAGACAG ATATGACTGT CATGGAGGAC 
GCAGTGGATG TCAGGGAGGA ACTGATGATT TCCTCATCAT TTGACAGTCT GGAGGTGCTG 
CTGGACTCAT TTGGCCCTGT GAGAGACTGT TCAAAAGACA ACGGAGGTTG CAGCCGAAAT 
TTCCGCTGTA TTTCTGATCG CAAGCTTGAC TCCACTGGCT GTGTGTGTCC TCCTGGATTG 
AGCCCGATGA AGGATGGCAC CGGTTGCTAT GACCACCACA TTGGCATTGA CTGCTCGGAC 
GGCTTTAATG GGGGCTGTGA GCAGCTGTGC CTCCAGCAGC TGGCACCTCT GGAGGAGGAT 
CCCACTCTCT ACAACATCCA GATGTTCTGC GGATGCATTG AAGATTATAA GAGAGGGTCA 
GATGGCCGCT CTTGTCAGCC ACTGTCTGAG GCCTGCACAG AGGGACTGGA CTGCGGGGAC 
ACAGCCGACA TCCCAGCCAA CCAGACTGTG TTTGGAGACC TCTTTTATGG CTATAATAAC 
CACACCAAAG AGATCACATC TGGACAGATA CTAAAAGCCA CTTTTAGGCA GAAAAACTTT 
GCTCGTGGCA TTGAGCAGCA GCTCCCGGAT GGCATGGTGG TGGCGTCAGT GCCGAATGAA 
GCCCAGTGTC ATGAGGAACT GTCTGACCCA GTGCCTGATC CAGAATACCT CACTGGTATG 
GTAAACTACA GTGAGGTTAC CGGTTACCCA TTGGTTCAGC ACTGGAGGCT GCGCTCTGTT 
ATGTACCATG TAAAGCTCAA TCAGTGGGCT CTTTCCCAAG CCTTCAGTTC TGCCATTCAT 
TCACTGGACG GGGCCACCCT GCGCAGCGAC TTTGTGTCCA TTTTAAAAGA GTTTGGCAAC 
CATTTTATCC AGGAGGCAGT GTATGGTTTT GAGGAGTCCT GTACCATATG GTATCCCAAC 
AAACAAGTCC AACGCCAGCT GTGGTTGGAG TACCAGGATA TCAGCAAAGG AAACTCTCCA 
TCGGACGAGT CGGAGGAGCG AGACAAAGAG CCACGCACAC TTACTTACCC AGCATACATT 
GCCAGCCTGC TGGACTCTGG TGCCAAGCGC ATGGCGGCGG GGGTGCGCAT GGACTGCAGC 
AGCCAGGGCC AGTGCCCTCT GTCCTGCCAC CTCTGCCACA TGAGCCCTGG GCCTGCACGT 
CCTGCAGAAC CTGTGCTGCT TAACATCACT AAAGCCACAC CGATCTTCGA ACTTGTCAAC 
AGCAACGAGA CGTACCAGGC TCTGCAGGAG GCCATGATGA GTGTTTTATG GTGCTCTGCT 
AAAGGGGATG TTATTGATGA CTGGTGCAGA TGTGACTCCA ATGCTTTTGG CACAGATGGA 
CTGCCAACCT GTGCTCCGCT GCCTCAACCT ATGCTCAAAC TGTCCCATTC GTATGAGCCC 
AGCAGCTCAT TGGTCATCAT AGAATGGAAT CACACAGAGC CACCAATTGG AGTGAGGATT 
GCTGATTACC TCATCAGTCA GGAGAAGGTG ACAGAGCGGA CTGATCACTC CAAAGTTGAG 
ACGGAGACAA TGTTGAGTTT TGTGGATGAC ATCATGTCAG GGGCCAAATC CCCGTGTGTG 
ATGCTTGGAG ACATTCCCGA TCCTCTGTCT TCAATCTCCT TGATCATTCG CTGCCTGGAG 
CCGGACACCA CCTACATGTT TCGGCTGTGG GCAGTGGACA ACACGGGTCG GCGCTCCAGT 
CCGAGTGAGG TGACGATCAA AACTCCTTGT CCTGCTGTGG ATGATGTCAA GGCTCAAGAA 
ATAGCAGATA AGATTTACAA TCTGTTCAAC GGCTACACCA GCGGGAAGGA GCAGCAGACG 
GCCTATAATA CACTCATGGA TCTGGGATCA CCAACCCTCC ACCGAGTTCT ATACCACTAC 
AATCAGCGCT ACGAGAGTTT TGGAGAGTTC ACCTGGCGAT GTGAAGATGA ACTTGGTCCA 
AGGAAGGCAG GACTGATCCT GTCTCAGCTG GATGAGCTCA GCGGTTGGTG TCGGGGGCTC 
CTCCAGGAAC CAAAGATTGG CCTGCGCAGA GCATCCCTCA AGTATCTGTC TTGTCGATAC 
ACAGATACCA AAGCGTTCGG GCTCAGCTGG GTGAACCTCG GCCAAGACTT GCGTAAGGCC 
TGCGAGGAGC AGATGCTGTC CGTGATGTAC AACGATTATG GAGAACCCAA GGAACTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

astn1 ( XM_003197882.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_003197930.4 Danio rerio astrotactin 1 (astn1), transcript variant X1, mRNA.

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CloneID	ODa56453
Clone ID Related Accession (Same CDS sequence)	XM_003197882.5 , XM_003197882.4
Accession Version	XM_003197882.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3960bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	astrotactin-1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_003197882.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGCTAC CGCCGCGCTC CTGTATGATG CTACCGCCGC GCTCCTATCT GCTTATCGGC 
GTTTTTTGGA TTGTCCTCTT CAGCTCTGCT GGCGTAAAAT GTCTGAACGG CGAAGAAGAT 
CAGTCTCAAG ACATGGAGTG CAAGATGAAA AGCGTTACGG TCTCCGCGTT GCCGTTTTTG 
CGAGAGAACG ACCTGAGCAT CATGCATAGC CCGTCGGCCT CCGAGCCCAA GCTACTCTTC 
TCCGTCAGGA ATGATTTTCC CGGCGAGATA GTCGTTGTTG ACGACTTGGA AAATACCGAG 
CTCCCGTACT TTGTGTTAGA GATCTCCGGG AACTCTGAAG ACATTCCTCT GGTGCGCTGG 
CGACAGCAGT GGTTGGAAAA TGGCACTTTG CTTTTCCACA TTCACCATCA GGACGGCAGT 
CAGAACTTGC CTGGTCCGGC GGCCACTGCT TACCCTGCTA GTGACTCTGC TGAAGAGGAG 
CTGCGCATTC TGCACATTTC TGTCATGGGT GGCATGATCG CTCTTCTGCT GTCCATCCTG 
TGCTTGGTGT TGATCCTGTA CACTCGCAGA CGCTGGTGTA AACGTCGCCG GGTTCCTCAA 
CCTCAAAAGA GCGCCAGTGC TGAAGCTGCC AATGAGATCC ACTACATCCC CTCAGTACTG 
ATGGGTGGAC AGGCCCGGGA AAGTCTGAGA AATTCGCGCG GGCAGGGCCA CAACTCGAGT 
GGCACCCTCA GCATCCGAGA GACCCCCATA CTGGATGGGT ACGAGTATGA CATCACTGAC 
CTGCGGCATC ACCTGCAGCG GGAGTGTATG AATGGTGGAG AGGACTTCAC CAGTCAGGTC 
ACCCGCACGC TAGACTCACT TCAGGGGTGC AATGAAAAGG GGGGCATGGA CCTCACTCCA 
GGGAGTGATA GCACAAAACT GTCCCTAATG AGCAAGTACA AGGATAACAT CATTGCTACC 
AGTCCTGTCG ACAGCAACCA CCAGCAAAAC ACACTTCTTC CACACAATAC CTCCACCAGC 
CAGCGCAAAA GGCTGTCCAG CAAAACACAC GCGGGTTCAA CTTTTCTGAA CCCAGATGGA 
GACTCTGGGA CAGAGGCGGA CAGCGAGCCT CAGCTGACTT TCTACACCGA CCCAAACCGC 
AGCCGCCGAC GCAGTCGAGG TATGAGAAAT GGTAACGTCA ACTCGGCGTG GGGCTCTGGG 
GGTTCCCCAC GGAGTCCTAT CAATAAGACC ACCCTCACTT TGATCAGCGT GGTGAGCTGT 
GTGATCGGCC TGGTCTGTGC GTCTCACCTT TCCTGTAGCC TGAACGTCAG AGTGATCCTT 
CACGTACCGG AGCATCTCAT TGCAGACGGC TCCAGGTTTG TTCTGCTTCA GGGCAGTCAG 
CTGGATGCCT CTGATTGGCT GAACCCTGCC CAGGTGCTGC TATACTACCA GCAGAATGCC 
AGCGGCCCCT GGGTCAACGA TCTGTGTGGC CGGCGTCTCC TGGACCCCTG TGAGCACCAG 
TGTGACCCAG ATACAGGAGA ATGTCTCTGC TTTGATGGCT ACATGAAAGA CCCAGTTCAC 
AAACACCTTT GCATCCGCAA TGAATGGGGC CCAAATCAAG GCCCATGGCC CTACACAATT 
TTCCAACGGG GCTTTGATTT GGTGATGGGA GAGCAGCCAT CGGATCGCAT TTTTAGATTT 
ACATACACTC TTGGAGAAGG CATGTGGCTG CCACTTAGCA AAAGCTTCGT CATTCCTCCG 
GCTGAGCTGG CCATCAACCC CTCTGCCAAG TGTAAGACAG ATATGACTGT CATGGAGGAC 
GCAGTGGATG TCAGGGAGGA ACTGATGATT TCCTCATCAT TTGACAGTCT GGAGGTGCTG 
CTGGACTCAT TTGGCCCTGT GAGAGACTGT TCAAAAGACA ACGGAGGTTG CAGCCGAAAT 
TTCCGCTGTA TTTCTGATCG CAAGCTTGAC TCCACTGGCT GTGTGTGTCC TCCTGGATTG 
AGCCCGATGA AGGATGGCAC CGGTTGCTAT GACCACCACA TTGGCATTGA CTGCTCGGAC 
GGCTTTAATG GGGGCTGTGA GCAGCTGTGC CTCCAGCAGC TGGCACCTCT GGAGGAGGAT 
CCCACTCTCT ACAACATCCA GATGTTCTGC GGATGCATTG AAGATTATAA GAGAGGGTCA 
GATGGCCGCT CTTGTCAGCC ACTGTCTGAG GCCTGCACAG AGGGACTGGA CTGCGGGGAC 
ACAGCCGACA TCCCAGCCAA CCAGACTGTG TTTGGAGACC TCTTTTATGG CTATAATAAC 
CACACCAAAG AGATCACATC TGGACAGATA CTAAAAGCCA CTTTTAGGCA GAAAAACTTT 
GCTCGTGGCA TTGAGCAGCA GCTCCCGGAT GGCATGGTGG TGGCGTCAGT GCCGAATGAA 
GCCCAGTGTC ATGAGGAACT GTCTGACCCA GTGCCTGATC CAGAATACCT CACTGGTATG 
GTAAACTACA GTGAGGTTAC CGGTTACCCA TTGGTTCAGC ACTGGAGGCT GCGCTCTGTT 
ATGTACCATG TAAAGCTCAA TCAGTGGGCT CTTTCCCAAG CCTTCAGTTC TGCCATTCAT 
TCACTGGACG GGGCCACCCT GCGCAGCGAC TTTGTGTCCA TTTTAAAAGA GTTTGGCAAC 
CATTTTATCC AGGAGGCAGT GTATGGTTTT GAGGAGTCCT GTACCATATG GTATCCCAAC 
AAACAAGTCC AACGCCAGCT GTGGTTGGAG TACCAGGATA TCAGCAAAGG AAACTCTCCA 
TCGGACGAGT CGGAGGAGCG AGACAAAGAG CCACGCACAC TTACTTACCC AGCATACATT 
GCCAGCCTGC TGGACTCTGG TGCCAAGCGC ATGGCGGCGG GGGTGCGCAT GGACTGCAGC 
AGCCAGGGCC AGTGCCCTCT GTCCTGCCAC CTCTGCCACA TGAGCCCTGG GCCTGCACGT 
CCTGCAGAAC CTGTGCTGCT TAACATCACT AAAGCCACAC CGATCTTCGA ACTTGTCAAC 
AGCAACGAGA CGTACCAGGC TCTGCAGGAG GCCATGATGA GTGTTTTATG GTGCTCTGCT 
AAAGGGGATG TTATTGATGA CTGGTGCAGA TGTGACTCCA ATGCTTTTGG CACAGATGGA 
CTGCCAACCT GTGCTCCGCT GCCTCAACCT ATGCTCAAAC TGTCCCATTC GTATGAGCCC 
AGCAGCTCAT TGGTCATCAT AGAATGGAAT CACACAGAGC CACCAATTGG AGTGAGGATT 
GCTGATTACC TCATCAGTCA GGAGAAGGTG ACAGAGCGGA CTGATCACTC CAAAGTTGAG 
ACGGAGACAA TGTTGAGTTT TGTGGATGAC ATCATGTCAG GGGCCAAATC CCCGTGTGTG 
ATGCTTGGAG ACATTCCCGA TCCTCTGTCT TCAATCTCCT TGATCATTCG CTGCCTGGAG 
CCGGACACCA CCTACATGTT TCGGCTGTGG GCAGTGGACA ACACGGGTCG GCGCTCCAGT 
CCGAGTGAGG TGACGATCAA AACTCCTTGT CCTGCTGTGG ATGATGTCAA GGCTCAAGAA 
ATAGCAGATA AGATTTACAA TCTGTTCAAC GGCTACACCA GCGGGAAGGA GCAGCAGACG 
GCCTATAATA CACTCATGGA TCTGGGATCA CCAACCCTCC ACCGAGTTCT ATACCACTAC 
AATCAGCGCT ACGAGAGTTT TGGAGAGTTC ACCTGGCGAT GTGAAGATGA ACTTGGTCCA 
AGGAAGGCAG GACTGATCCT GTCTCAGCTG GATGAGCTCA GCGGTTGGTG TCGGGGGCTC 
CTCCAGGAAC CAAAGATTGG CCTGCGCAGA GCATCCCTCA AGTATCTGTC TTGTCGATAC 
ACAGATACCA AAGCGTTCGG GCTCAGCTGG GTGAACCTCG GCCAAGACTT GCGTAAGGCC 
TGCGAGGAGC AGATGCTGTC CGTGATGTAC AACGATTATG GAGAACCCAA GGAACTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003197930.4
CDS	369..4328
Translation	MMLPPRSCMMLPPRSYLLIGVFWIVLFSSAGVKCLNGEEDQSQDMECKMKSVTVSALPFLRENDLSIMHSPSASEPKLLFSVRNDFPGEIVVVDDLENTELPYFVLEISGNSEDIPLVRWRQQWLENGTLLFHIHHQDGSQNLPGPAATAYPASDSAEEELRILHISVMGGMIALLLSILCLVLILYTRRRWCKRRRVPQPQKSASAEAANEIHYIPSVLMGGQARESLRNSRGQGHNSSGTLSIRETPILDGYEYDITDLRHHLQRECMNGGEDFTSQVTRTLDSLQGCNEKGGMDLTPGSDSTKLSLMSKYKDNIIATSPVDSNHQQNTLLPHNTSTSQRKRLSSKTHAGSTFLNPDGDSGTEADSEPQLTFYTDPNRSRRRSRGMRNGNVNSAWGSGGSPRSPINKTTLTLISVVSCVIGLVCASHLSCSLNVRVILHVPEHLIADGSRFVLLQGSQLDASDWLNPAQVLLYYQQNASGPWVNDLCGRRLLDPCEHQCDPDTGECLCFDGYMKDPVHKHLCIRNEWGPNQGPWPYTIFQRGFDLVMGEQPSDRIFRFTYTLGEGMWLPLSKSFVIPPAELAINPSAKCKTDMTVMEDAVDVREELMISSSFDSLEVLLDSFGPVRDCSKDNGGCSRNFRCISDRKLDSTGCVCPPGLSPMKDGTGCYDHHIGIDCSDGFNGGCEQLCLQQLAPLEEDPTLYNIQMFCGCIEDYKRGSDGRSCQPLSEACTEGLDCGDTADIPANQTVFGDLFYGYNNHTKEITSGQILKATFRQKNFARGIEQQLPDGMVVASVPNEAQCHEELSDPVPDPEYLTGMVNYSEVTGYPLVQHWRLRSVMYHVKLNQWALSQAFSSAIHSLDGATLRSDFVSILKEFGNHFIQEAVYGFEESCTIWYPNKQVQRQLWLEYQDISKGNSPSDESEERDKEPRTLTYPAYIASLLDSGAKRMAAGVRMDCSSQGQCPLSCHLCHMSPGPARPAEPVLLNITKATPIFELVNSNETYQALQEAMMSVLWCSAKGDVIDDWCRCDSNAFGTDGLPTCAPLPQPMLKLSHSYEPSSSLVIIEWNHTEPPIGVRIADYLISQEKVTERTDHSKVETETMLSFVDDIMSGAKSPCVMLGDIPDPLSSISLIIRCLEPDTTYMFRLWAVDNTGRRSSPSEVTIKTPCPAVDDVKAQEIADKIYNLFNGYTSGKEQQTAYNTLMDLGSPTLHRVLYHYNQRYESFGEFTWRCEDELGPRKAGLILSQLDELSGWCRGLLQEPKIGLRRASLKYLSCRYTDTKAFGLSWVNLGQDLRKACEEQMLSVMYNDYGEPKEL

Target ORF information:

RefSeq Version	XM_003197882.4
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio astrotactin 1 (astn1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003197882.4

ORF Insert Sequence:

ATGATGCTAC CGCCGCGCTC CTGTATGATG CTACCGCCGC GCTCCTATCT GCTTATCGGC 
GTTTTTTGGA TTGTCCTCTT CAGCTCTGCT GGCGTAAAAT GTCTGAACGG CGAAGAAGAT 
CAGTCTCAAG ACATGGAGTG CAAGATGAAA AGCGTTACGG TCTCCGCGTT GCCGTTTTTG 
CGAGAGAACG ACCTGAGCAT CATGCATAGC CCGTCGGCCT CCGAGCCCAA GCTACTCTTC 
TCCGTCAGGA ATGATTTTCC CGGCGAGATA GTCGTTGTTG ACGACTTGGA AAATACCGAG 
CTCCCGTACT TTGTGTTAGA GATCTCCGGG AACTCTGAAG ACATTCCTCT GGTGCGCTGG 
CGACAGCAGT GGTTGGAAAA TGGCACTTTG CTTTTCCACA TTCACCATCA GGACGGCAGT 
CAGAACTTGC CTGGTCCGGC GGCCACTGCT TACCCTGCTA GTGACTCTGC TGAAGAGGAG 
CTGCGCATTC TGCACATTTC TGTCATGGGT GGCATGATCG CTCTTCTGCT GTCCATCCTG 
TGCTTGGTGT TGATCCTGTA CACTCGCAGA CGCTGGTGTA AACGTCGCCG GGTTCCTCAA 
CCTCAAAAGA GCGCCAGTGC TGAAGCTGCC AATGAGATCC ACTACATCCC CTCAGTACTG 
ATGGGTGGAC AGGCCCGGGA AAGTCTGAGA AATTCGCGCG GGCAGGGCCA CAACTCGAGT 
GGCACCCTCA GCATCCGAGA GACCCCCATA CTGGATGGGT ACGAGTATGA CATCACTGAC 
CTGCGGCATC ACCTGCAGCG GGAGTGTATG AATGGTGGAG AGGACTTCAC CAGTCAGGTC 
ACCCGCACGC TAGACTCACT TCAGGGGTGC AATGAAAAGG GGGGCATGGA CCTCACTCCA 
GGGAGTGATA GCACAAAACT GTCCCTAATG AGCAAGTACA AGGATAACAT CATTGCTACC 
AGTCCTGTCG ACAGCAACCA CCAGCAAAAC ACACTTCTTC CACACAATAC CTCCACCAGC 
CAGCGCAAAA GGCTGTCCAG CAAAACACAC GCGGGTTCAA CTTTTCTGAA CCCAGATGGA 
GACTCTGGGA CAGAGGCGGA CAGCGAGCCT CAGCTGACTT TCTACACCGA CCCAAACCGC 
AGCCGCCGAC GCAGTCGAGG TATGAGAAAT GGTAACGTCA ACTCGGCGTG GGGCTCTGGG 
GGTTCCCCAC GGAGTCCTAT CAATAAGACC ACCCTCACTT TGATCAGCGT GGTGAGCTGT 
GTGATCGGCC TGGTCTGTGC GTCTCACCTT TCCTGTAGCC TGAACGTCAG AGTGATCCTT 
CACGTACCGG AGCATCTCAT TGCAGACGGC TCCAGGTTTG TTCTGCTTCA GGGCAGTCAG 
CTGGATGCCT CTGATTGGCT GAACCCTGCC CAGGTGCTGC TATACTACCA GCAGAATGCC 
AGCGGCCCCT GGGTCAACGA TCTGTGTGGC CGGCGTCTCC TGGACCCCTG TGAGCACCAG 
TGTGACCCAG ATACAGGAGA ATGTCTCTGC TTTGATGGCT ACATGAAAGA CCCAGTTCAC 
AAACACCTTT GCATCCGCAA TGAATGGGGC CCAAATCAAG GCCCATGGCC CTACACAATT 
TTCCAACGGG GCTTTGATTT GGTGATGGGA GAGCAGCCAT CGGATCGCAT TTTTAGATTT 
ACATACACTC TTGGAGAAGG CATGTGGCTG CCACTTAGCA AAAGCTTCGT CATTCCTCCG 
GCTGAGCTGG CCATCAACCC CTCTGCCAAG TGTAAGACAG ATATGACTGT CATGGAGGAC 
GCAGTGGATG TCAGGGAGGA ACTGATGATT TCCTCATCAT TTGACAGTCT GGAGGTGCTG 
CTGGACTCAT TTGGCCCTGT GAGAGACTGT TCAAAAGACA ACGGAGGTTG CAGCCGAAAT 
TTCCGCTGTA TTTCTGATCG CAAGCTTGAC TCCACTGGCT GTGTGTGTCC TCCTGGATTG 
AGCCCGATGA AGGATGGCAC CGGTTGCTAT GACCACCACA TTGGCATTGA CTGCTCGGAC 
GGCTTTAATG GGGGCTGTGA GCAGCTGTGC CTCCAGCAGC TGGCACCTCT GGAGGAGGAT 
CCCACTCTCT ACAACATCCA GATGTTCTGC GGATGCATTG AAGATTATAA GAGAGGGTCA 
GATGGCCGCT CTTGTCAGCC ACTGTCTGAG GCCTGCACAG AGGGACTGGA CTGCGGGGAC 
ACAGCCGACA TCCCAGCCAA CCAGACTGTG TTTGGAGACC TCTTTTATGG CTATAATAAC 
CACACCAAAG AGATCACATC TGGACAGATA CTAAAAGCCA CTTTTAGGCA GAAAAACTTT 
GCTCGTGGCA TTGAGCAGCA GCTCCCGGAT GGCATGGTGG TGGCGTCAGT GCCGAATGAA 
GCCCAGTGTC ATGAGGAACT GTCTGACCCA GTGCCTGATC CAGAATACCT CACTGGTATG 
GTAAACTACA GTGAGGTTAC CGGTTACCCA TTGGTTCAGC ACTGGAGGCT GCGCTCTGTT 
ATGTACCATG TAAAGCTCAA TCAGTGGGCT CTTTCCCAAG CCTTCAGTTC TGCCATTCAT 
TCACTGGACG GGGCCACCCT GCGCAGCGAC TTTGTGTCCA TTTTAAAAGA GTTTGGCAAC 
CATTTTATCC AGGAGGCAGT GTATGGTTTT GAGGAGTCCT GTACCATATG GTATCCCAAC 
AAACAAGTCC AACGCCAGCT GTGGTTGGAG TACCAGGATA TCAGCAAAGG AAACTCTCCA 
TCGGACGAGT CGGAGGAGCG AGACAAAGAG CCACGCACAC TTACTTACCC AGCATACATT 
GCCAGCCTGC TGGACTCTGG TGCCAAGCGC ATGGCGGCGG GGGTGCGCAT GGACTGCAGC 
AGCCAGGGCC AGTGCCCTCT GTCCTGCCAC CTCTGCCACA TGAGCCCTGG GCCTGCACGT 
CCTGCAGAAC CTGTGCTGCT TAACATCACT AAAGCCACAC CGATCTTCGA ACTTGTCAAC 
AGCAACGAGA CGTACCAGGC TCTGCAGGAG GCCATGATGA GTGTTTTATG GTGCTCTGCT 
AAAGGGGATG TTATTGATGA CTGGTGCAGA TGTGACTCCA ATGCTTTTGG CACAGATGGA 
CTGCCAACCT GTGCTCCGCT GCCTCAACCT ATGCTCAAAC TGTCCCATTC GTATGAGCCC 
AGCAGCTCAT TGGTCATCAT AGAATGGAAT CACACAGAGC CACCAATTGG AGTGAGGATT 
GCTGATTACC TCATCAGTCA GGAGAAGGTG ACAGAGCGGA CTGATCACTC CAAAGTTGAG 
ACGGAGACAA TGTTGAGTTT TGTGGATGAC ATCATGTCAG GGGCCAAATC CCCGTGTGTG 
ATGCTTGGAG ACATTCCCGA TCCTCTGTCT TCAATCTCCT TGATCATTCG CTGCCTGGAG 
CCGGACACCA CCTACATGTT TCGGCTGTGG GCAGTGGACA ACACGGGTCG GCGCTCCAGT 
CCGAGTGAGG TGACGATCAA AACTCCTTGT CCTGCTGTGG ATGATGTCAA GGCTCAAGAA 
ATAGCAGATA AGATTTACAA TCTGTTCAAC GGCTACACCA GCGGGAAGGA GCAGCAGACG 
GCCTATAATA CACTCATGGA TCTGGGATCA CCAACCCTCC ACCGAGTTCT ATACCACTAC 
AATCAGCGCT ACGAGAGTTT TGGAGAGTTC ACCTGGCGAT GTGAAGATGA ACTTGGTCCA 
AGGAAGGCAG GACTGATCCT GTCTCAGCTG GATGAGCTCA GCGGTTGGTG TCGGGGGCTC 
CTCCAGGAAC CAAAGATTGG CCTGCGCAGA GCATCCCTCA AGTATCTGTC TTGTCGATAC 
ACAGATACCA AAGCGTTCGG GCTCAGCTGG GTGAACCTCG GCCAAGACTT GCGTAAGGCC 
TGCGAGGAGC AGATGCTGTC CGTGATGTAC AACGATTATG GAGAACCCAA GGAACTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

astn1 ( XM_017351897.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017207386.1 Danio rerio astrotactin 1 (astn1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa56454
Clone ID Related Accession (Same CDS sequence)	XM_017351897.1 , XM_017351897.2
Accession Version	XM_017351897.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3918bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	astrotactin-1 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGCTAC CGCCGCGCTC CTGTATGATG CTACCGCCGC GCTCCTATCT GCTTATCGGC 
GTTTTTTGGA TTGTCCTCTT CAGCTCTGCT GGCGTAAAAT GTCTGAACGG CGAAGAAGAT 
CAGTCTCAAG ACATGGAGTG CAAGATGAAA AGCGTTACGG TCTCCGCGTT GCCGTTTTTG 
CGAGAGAACG ACCTGAGCAT CATGCATAGC CCGTCGGCCT CCGAGCCCAA GCTACTCTTC 
TCCGTCAGGA ATGATTTTCC CGGCGAGATA GTCGTTGTTG ACGACTTGGA AAATACCGAG 
CTCCCGTACT TTGTGTTAGA GATCTCCGGG AACTCTGAAG ACATTCCTCT GGTGCGCTGG 
CGACAGCAGT GGTTGGAAAA TGGCACTTTG CTTTTCCACA TTCACCATCA GGACGGCAGT 
CAGAACTTGC CTGGTCCGGC GGCCACTGCT TACCCTGCTA GTGACTCTGC TGAAGAGGAG 
CTGCGCATTC TGCACATTTC TGTCATGGGT GGCATGATCG CTCTTCTGCT GTCCATCCTG 
TGCTTGGTGT TGATCCTGTA CACTCGCAGA CGCTGGTGTA AACGTCGCCG GGTTCCTCAA 
CCTCAAAAGA GCGCCAGTGC TGAAGCTGCC AATGAGATCC ACTACATCCC CTCAGTACTG 
ATGGGTGGAC AGGCCCGGGA AAGTCTGAGA AATTCGCGCG GGCAGGGCCA CAACTCGAGT 
GGCACCCTCA GCATCCGAGA GACCCCCATA CTGGATGGGT ACGAGTATGA CATCACTGAC 
CTGCGGCATC ACCTGCAGCG GGAGTGTATG AATGGTGGAG AGGACTTCAC CAGTCAGGTC 
ACCCGCACGC TAGACTCACT TCAGGGGTGC AATGAAAAGG GGGGCATGGA CCTCACTCCA 
GGGAGTGATA GCACAAAACT GTCCCTAATG AGCAAGTACA AGGATAACAT CATTGCTACC 
AGTCCTGTCG ACAGCAACCA CCAGCAAAAC ACACTTCTTC CACACAATAC CTCCACCAGC 
CAGCGCAAAA GGCTGTCCAG CAAAACACAC GCGGGTTCAA CTTTTCTGAA CCCAGATGGA 
GACTCTGGGA CAGAGGCGGA CAGCGAGCCT CAGCTGACTT TCTACACCGA CCCAAACCGC 
AGCCGCCGAC GCAGTCGAGG TTCCCCACGG AGTCCTATCA ATAAGACCAC CCTCACTTTG 
ATCAGCGTGG TGAGCTGTGT GATCGGCCTG GTCTGTGCGT CTCACCTTTC CTGTAGCCTG 
AACGTCAGAG TGATCCTTCA CGTACCGGAG CATCTCATTG CAGACGGCTC CAGGTTTGTT 
CTGCTTCAGG GCAGTCAGCT GGATGCCTCT GATTGGCTGA ACCCTGCCCA GGTGCTGCTA 
TACTACCAGC AGAATGCCAG CGGCCCCTGG GTCAACGATC TGTGTGGCCG GCGTCTCCTG 
GACCCCTGTG AGCACCAGTG TGACCCAGAT ACAGGAGAAT GTCTCTGCTT TGATGGCTAC 
ATGAAAGACC CAGTTCACAA ACACCTTTGC ATCCGCAATG AATGGGGCCC AAATCAAGGC 
CCATGGCCCT ACACAATTTT CCAACGGGGC TTTGATTTGG TGATGGGAGA GCAGCCATCG 
GATCGCATTT TTAGATTTAC ATACACTCTT GGAGAAGGCA TGTGGCTGCC ACTTAGCAAA 
AGCTTCGTCA TTCCTCCGGC TGAGCTGGCC ATCAACCCCT CTGCCAAGTG TAAGACAGAT 
ATGACTGTCA TGGAGGACGC AGTGGATGTC AGGGAGGAAC TGATGATTTC CTCATCATTT 
GACAGTCTGG AGGTGCTGCT GGACTCATTT GGCCCTGTGA GAGACTGTTC AAAAGACAAC 
GGAGGTTGCA GCCGAAATTT CCGCTGTATT TCTGATCGCA AGCTTGACTC CACTGGCTGT 
GTGTGTCCTC CTGGATTGAG CCCGATGAAG GATGGCACCG GTTGCTATGA CCACCACATT 
GGCATTGACT GCTCGGACGG CTTTAATGGG GGCTGTGAGC AGCTGTGCCT CCAGCAGCTG 
GCACCTCTGG AGGAGGATCC CACTCTCTAC AACATCCAGA TGTTCTGCGG ATGCATTGAA 
GATTATAAGA GAGGGTCAGA TGGCCGCTCT TGTCAGCCAC TGTCTGAGGC CTGCACAGAG 
GGACTGGACT GCGGGGACAC AGCCGACATC CCAGCCAACC AGACTGTGTT TGGAGACCTC 
TTTTATGGCT ATAATAACCA CACCAAAGAG ATCACATCTG GACAGATACT AAAAGCCACT 
TTTAGGCAGA AAAACTTTGC TCGTGGCATT GAGCAGCAGC TCCCGGATGG CATGGTGGTG 
GCGTCAGTGC CGAATGAAGC CCAGTGTCAT GAGGAACTGT CTGACCCAGT GCCTGATCCA 
GAATACCTCA CTGGTATGGT AAACTACAGT GAGGTTACCG GTTACCCATT GGTTCAGCAC 
TGGAGGCTGC GCTCTGTTAT GTACCATGTA AAGCTCAATC AGTGGGCTCT TTCCCAAGCC 
TTCAGTTCTG CCATTCATTC ACTGGACGGG GCCACCCTGC GCAGCGACTT TGTGTCCATT 
TTAAAAGAGT TTGGCAACCA TTTTATCCAG GAGGCAGTGT ATGGTTTTGA GGAGTCCTGT 
ACCATATGGT ATCCCAACAA ACAAGTCCAA CGCCAGCTGT GGTTGGAGTA CCAGGATATC 
AGCAAAGGAA ACTCTCCATC GGACGAGTCG GAGGAGCGAG ACAAAGAGCC ACGCACACTT 
ACTTACCCAG CATACATTGC CAGCCTGCTG GACTCTGGTG CCAAGCGCAT GGCGGCGGGG 
GTGCGCATGG ACTGCAGCAG CCAGGGCCAG TGCCCTCTGT CCTGCCACCT CTGCCACATG 
AGCCCTGGGC CTGCACGTCC TGCAGAACCT GTGCTGCTTA ACATCACTAA AGCCACACCG 
ATCTTCGAAC TTGTCAACAG CAACGAGACG TACCAGGCTC TGCAGGAGGC CATGATGAGT 
GTTTTATGGT GCTCTGCTAA AGGGGATGTT ATTGATGACT GGTGCAGATG TGACTCCAAT 
GCTTTTGGCA CAGATGGACT GCCAACCTGT GCTCCGCTGC CTCAACCTAT GCTCAAACTG 
TCCCATTCGT ATGAGCCCAG CAGCTCATTG GTCATCATAG AATGGAATCA CACAGAGCCA 
CCAATTGGAG TGAGGATTGC TGATTACCTC ATCAGTCAGG AGAAGGTGAC AGAGCGGACT 
GATCACTCCA AAGTTGAGAC GGAGACAATG TTGAGTTTTG TGGATGACAT CATGTCAGGG 
GCCAAATCCC CGTGTGTGAT GCTTGGAGAC ATTCCCGATC CTCTGTCTTC AATCTCCTTG 
ATCATTCGCT GCCTGGAGCC GGACACCACC TACATGTTTC GGCTGTGGGC AGTGGACAAC 
ACGGGTCGGC GCTCCAGTCC GAGTGAGGTG ACGATCAAAA CTCCTTGTCC TGCTGTGGAT 
GATGTCAAGG CTCAAGAAAT AGCAGATAAG ATTTACAATC TGTTCAACGG CTACACCAGC 
GGGAAGGAGC AGCAGACGGC CTATAATACA CTCATGGATC TGGGATCACC AACCCTCCAC 
CGAGTTCTAT ACCACTACAA TCAGCGCTAC GAGAGTTTTG GAGAGTTCAC CTGGCGATGT 
GAAGATGAAC TTGGTCCAAG GAAGGCAGGA CTGATCCTGT CTCAGCTGGA TGAGCTCAGC 
GGTTGGTGTC GGGGGCTCCT CCAGGAACCA AAGATTGGCC TGCGCAGAGC ATCCCTCAAG 
TATCTGTCTT GTCGATACAC AGATACCAAA GCGTTCGGGC TCAGCTGGGT GAACCTCGGC 
CAAGACTTGC GTAAGGCCTG CGAGGAGCAG ATGCTGTCCG TGATGTACAA CGATTATGGA 
GAACCCAAGG AACTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017207386.1
CDS	368..4285
Translation	MMLPPRSCMMLPPRSYLLIGVFWIVLFSSAGVKCLNGEEDQSQDMECKMKSVTVSALPFLRENDLSIMHSPSASEPKLLFSVRNDFPGEIVVVDDLENTELPYFVLEISGNSEDIPLVRWRQQWLENGTLLFHIHHQDGSQNLPGPAATAYPASDSAEEELRILHISVMGGMIALLLSILCLVLILYTRRRWCKRRRVPQPQKSASAEAANEIHYIPSVLMGGQARESLRNSRGQGHNSSGTLSIRETPILDGYEYDITDLRHHLQRECMNGGEDFTSQVTRTLDSLQGCNEKGGMDLTPGSDSTKLSLMSKYKDNIIATSPVDSNHQQNTLLPHNTSTSQRKRLSSKTHAGSTFLNPDGDSGTEADSEPQLTFYTDPNRSRRRSRGSPRSPINKTTLTLISVVSCVIGLVCASHLSCSLNVRVILHVPEHLIADGSRFVLLQGSQLDASDWLNPAQVLLYYQQNASGPWVNDLCGRRLLDPCEHQCDPDTGECLCFDGYMKDPVHKHLCIRNEWGPNQGPWPYTIFQRGFDLVMGEQPSDRIFRFTYTLGEGMWLPLSKSFVIPPAELAINPSAKCKTDMTVMEDAVDVREELMISSSFDSLEVLLDSFGPVRDCSKDNGGCSRNFRCISDRKLDSTGCVCPPGLSPMKDGTGCYDHHIGIDCSDGFNGGCEQLCLQQLAPLEEDPTLYNIQMFCGCIEDYKRGSDGRSCQPLSEACTEGLDCGDTADIPANQTVFGDLFYGYNNHTKEITSGQILKATFRQKNFARGIEQQLPDGMVVASVPNEAQCHEELSDPVPDPEYLTGMVNYSEVTGYPLVQHWRLRSVMYHVKLNQWALSQAFSSAIHSLDGATLRSDFVSILKEFGNHFIQEAVYGFEESCTIWYPNKQVQRQLWLEYQDISKGNSPSDESEERDKEPRTLTYPAYIASLLDSGAKRMAAGVRMDCSSQGQCPLSCHLCHMSPGPARPAEPVLLNITKATPIFELVNSNETYQALQEAMMSVLWCSAKGDVIDDWCRCDSNAFGTDGLPTCAPLPQPMLKLSHSYEPSSSLVIIEWNHTEPPIGVRIADYLISQEKVTERTDHSKVETETMLSFVDDIMSGAKSPCVMLGDIPDPLSSISLIIRCLEPDTTYMFRLWAVDNTGRRSSPSEVTIKTPCPAVDDVKAQEIADKIYNLFNGYTSGKEQQTAYNTLMDLGSPTLHRVLYHYNQRYESFGEFTWRCEDELGPRKAGLILSQLDELSGWCRGLLQEPKIGLRRASLKYLSCRYTDTKAFGLSWVNLGQDLRKACEEQMLSVMYNDYGEPKEL

Target ORF information:

RefSeq Version	XM_017351897.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio astrotactin 1 (astn1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017351897.1

ORF Insert Sequence:

ATGATGCTAC CGCCGCGCTC CTGTATGATG CTACCGCCGC GCTCCTATCT GCTTATCGGC 
GTTTTTTGGA TTGTCCTCTT CAGCTCTGCT GGCGTAAAAT GTCTGAACGG CGAAGAAGAT 
CAGTCTCAAG ACATGGAGTG CAAGATGAAA AGCGTTACGG TCTCCGCGTT GCCGTTTTTG 
CGAGAGAACG ACCTGAGCAT CATGCATAGC CCGTCGGCCT CCGAGCCCAA GCTACTCTTC 
TCCGTCAGGA ATGATTTTCC CGGCGAGATA GTCGTTGTTG ACGACTTGGA AAATACCGAG 
CTCCCGTACT TTGTGTTAGA GATCTCCGGG AACTCTGAAG ACATTCCTCT GGTGCGCTGG 
CGACAGCAGT GGTTGGAAAA TGGCACTTTG CTTTTCCACA TTCACCATCA GGACGGCAGT 
CAGAACTTGC CTGGTCCGGC GGCCACTGCT TACCCTGCTA GTGACTCTGC TGAAGAGGAG 
CTGCGCATTC TGCACATTTC TGTCATGGGT GGCATGATCG CTCTTCTGCT GTCCATCCTG 
TGCTTGGTGT TGATCCTGTA CACTCGCAGA CGCTGGTGTA AACGTCGCCG GGTTCCTCAA 
CCTCAAAAGA GCGCCAGTGC TGAAGCTGCC AATGAGATCC ACTACATCCC CTCAGTACTG 
ATGGGTGGAC AGGCCCGGGA AAGTCTGAGA AATTCGCGCG GGCAGGGCCA CAACTCGAGT 
GGCACCCTCA GCATCCGAGA GACCCCCATA CTGGATGGGT ACGAGTATGA CATCACTGAC 
CTGCGGCATC ACCTGCAGCG GGAGTGTATG AATGGTGGAG AGGACTTCAC CAGTCAGGTC 
ACCCGCACGC TAGACTCACT TCAGGGGTGC AATGAAAAGG GGGGCATGGA CCTCACTCCA 
GGGAGTGATA GCACAAAACT GTCCCTAATG AGCAAGTACA AGGATAACAT CATTGCTACC 
AGTCCTGTCG ACAGCAACCA CCAGCAAAAC ACACTTCTTC CACACAATAC CTCCACCAGC 
CAGCGCAAAA GGCTGTCCAG CAAAACACAC GCGGGTTCAA CTTTTCTGAA CCCAGATGGA 
GACTCTGGGA CAGAGGCGGA CAGCGAGCCT CAGCTGACTT TCTACACCGA CCCAAACCGC 
AGCCGCCGAC GCAGTCGAGG TTCCCCACGG AGTCCTATCA ATAAGACCAC CCTCACTTTG 
ATCAGCGTGG TGAGCTGTGT GATCGGCCTG GTCTGTGCGT CTCACCTTTC CTGTAGCCTG 
AACGTCAGAG TGATCCTTCA CGTACCGGAG CATCTCATTG CAGACGGCTC CAGGTTTGTT 
CTGCTTCAGG GCAGTCAGCT GGATGCCTCT GATTGGCTGA ACCCTGCCCA GGTGCTGCTA 
TACTACCAGC AGAATGCCAG CGGCCCCTGG GTCAACGATC TGTGTGGCCG GCGTCTCCTG 
GACCCCTGTG AGCACCAGTG TGACCCAGAT ACAGGAGAAT GTCTCTGCTT TGATGGCTAC 
ATGAAAGACC CAGTTCACAA ACACCTTTGC ATCCGCAATG AATGGGGCCC AAATCAAGGC 
CCATGGCCCT ACACAATTTT CCAACGGGGC TTTGATTTGG TGATGGGAGA GCAGCCATCG 
GATCGCATTT TTAGATTTAC ATACACTCTT GGAGAAGGCA TGTGGCTGCC ACTTAGCAAA 
AGCTTCGTCA TTCCTCCGGC TGAGCTGGCC ATCAACCCCT CTGCCAAGTG TAAGACAGAT 
ATGACTGTCA TGGAGGACGC AGTGGATGTC AGGGAGGAAC TGATGATTTC CTCATCATTT 
GACAGTCTGG AGGTGCTGCT GGACTCATTT GGCCCTGTGA GAGACTGTTC AAAAGACAAC 
GGAGGTTGCA GCCGAAATTT CCGCTGTATT TCTGATCGCA AGCTTGACTC CACTGGCTGT 
GTGTGTCCTC CTGGATTGAG CCCGATGAAG GATGGCACCG GTTGCTATGA CCACCACATT 
GGCATTGACT GCTCGGACGG CTTTAATGGG GGCTGTGAGC AGCTGTGCCT CCAGCAGCTG 
GCACCTCTGG AGGAGGATCC CACTCTCTAC AACATCCAGA TGTTCTGCGG ATGCATTGAA 
GATTATAAGA GAGGGTCAGA TGGCCGCTCT TGTCAGCCAC TGTCTGAGGC CTGCACAGAG 
GGACTGGACT GCGGGGACAC AGCCGACATC CCAGCCAACC AGACTGTGTT TGGAGACCTC 
TTTTATGGCT ATAATAACCA CACCAAAGAG ATCACATCTG GACAGATACT AAAAGCCACT 
TTTAGGCAGA AAAACTTTGC TCGTGGCATT GAGCAGCAGC TCCCGGATGG CATGGTGGTG 
GCGTCAGTGC CGAATGAAGC CCAGTGTCAT GAGGAACTGT CTGACCCAGT GCCTGATCCA 
GAATACCTCA CTGGTATGGT AAACTACAGT GAGGTTACCG GTTACCCATT GGTTCAGCAC 
TGGAGGCTGC GCTCTGTTAT GTACCATGTA AAGCTCAATC AGTGGGCTCT TTCCCAAGCC 
TTCAGTTCTG CCATTCATTC ACTGGACGGG GCCACCCTGC GCAGCGACTT TGTGTCCATT 
TTAAAAGAGT TTGGCAACCA TTTTATCCAG GAGGCAGTGT ATGGTTTTGA GGAGTCCTGT 
ACCATATGGT ATCCCAACAA ACAAGTCCAA CGCCAGCTGT GGTTGGAGTA CCAGGATATC 
AGCAAAGGAA ACTCTCCATC GGACGAGTCG GAGGAGCGAG ACAAAGAGCC ACGCACACTT 
ACTTACCCAG CATACATTGC CAGCCTGCTG GACTCTGGTG CCAAGCGCAT GGCGGCGGGG 
GTGCGCATGG ACTGCAGCAG CCAGGGCCAG TGCCCTCTGT CCTGCCACCT CTGCCACATG 
AGCCCTGGGC CTGCACGTCC TGCAGAACCT GTGCTGCTTA ACATCACTAA AGCCACACCG 
ATCTTCGAAC TTGTCAACAG CAACGAGACG TACCAGGCTC TGCAGGAGGC CATGATGAGT 
GTTTTATGGT GCTCTGCTAA AGGGGATGTT ATTGATGACT GGTGCAGATG TGACTCCAAT 
GCTTTTGGCA CAGATGGACT GCCAACCTGT GCTCCGCTGC CTCAACCTAT GCTCAAACTG 
TCCCATTCGT ATGAGCCCAG CAGCTCATTG GTCATCATAG AATGGAATCA CACAGAGCCA 
CCAATTGGAG TGAGGATTGC TGATTACCTC ATCAGTCAGG AGAAGGTGAC AGAGCGGACT 
GATCACTCCA AAGTTGAGAC GGAGACAATG TTGAGTTTTG TGGATGACAT CATGTCAGGG 
GCCAAATCCC CGTGTGTGAT GCTTGGAGAC ATTCCCGATC CTCTGTCTTC AATCTCCTTG 
ATCATTCGCT GCCTGGAGCC GGACACCACC TACATGTTTC GGCTGTGGGC AGTGGACAAC 
ACGGGTCGGC GCTCCAGTCC GAGTGAGGTG ACGATCAAAA CTCCTTGTCC TGCTGTGGAT 
GATGTCAAGG CTCAAGAAAT AGCAGATAAG ATTTACAATC TGTTCAACGG CTACACCAGC 
GGGAAGGAGC AGCAGACGGC CTATAATACA CTCATGGATC TGGGATCACC AACCCTCCAC 
CGAGTTCTAT ACCACTACAA TCAGCGCTAC GAGAGTTTTG GAGAGTTCAC CTGGCGATGT 
GAAGATGAAC TTGGTCCAAG GAAGGCAGGA CTGATCCTGT CTCAGCTGGA TGAGCTCAGC 
GGTTGGTGTC GGGGGCTCCT CCAGGAACCA AAGATTGGCC TGCGCAGAGC ATCCCTCAAG 
TATCTGTCTT GTCGATACAC AGATACCAAA GCGTTCGGGC TCAGCTGGGT GAACCTCGGC 
CAAGACTTGC GTAAGGCCTG CGAGGAGCAG ATGCTGTCCG TGATGTACAA CGATTATGGA 
GAACCCAAGG AACTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

astn1 ( XM_017351897.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017207386.1 Danio rerio astrotactin 1 (astn1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa56454
Clone ID Related Accession (Same CDS sequence)	XM_017351897.1 , XM_017351897.2
Accession Version	XM_017351897.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3918bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	astrotactin-1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017351897.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGCTAC CGCCGCGCTC CTGTATGATG CTACCGCCGC GCTCCTATCT GCTTATCGGC 
GTTTTTTGGA TTGTCCTCTT CAGCTCTGCT GGCGTAAAAT GTCTGAACGG CGAAGAAGAT 
CAGTCTCAAG ACATGGAGTG CAAGATGAAA AGCGTTACGG TCTCCGCGTT GCCGTTTTTG 
CGAGAGAACG ACCTGAGCAT CATGCATAGC CCGTCGGCCT CCGAGCCCAA GCTACTCTTC 
TCCGTCAGGA ATGATTTTCC CGGCGAGATA GTCGTTGTTG ACGACTTGGA AAATACCGAG 
CTCCCGTACT TTGTGTTAGA GATCTCCGGG AACTCTGAAG ACATTCCTCT GGTGCGCTGG 
CGACAGCAGT GGTTGGAAAA TGGCACTTTG CTTTTCCACA TTCACCATCA GGACGGCAGT 
CAGAACTTGC CTGGTCCGGC GGCCACTGCT TACCCTGCTA GTGACTCTGC TGAAGAGGAG 
CTGCGCATTC TGCACATTTC TGTCATGGGT GGCATGATCG CTCTTCTGCT GTCCATCCTG 
TGCTTGGTGT TGATCCTGTA CACTCGCAGA CGCTGGTGTA AACGTCGCCG GGTTCCTCAA 
CCTCAAAAGA GCGCCAGTGC TGAAGCTGCC AATGAGATCC ACTACATCCC CTCAGTACTG 
ATGGGTGGAC AGGCCCGGGA AAGTCTGAGA AATTCGCGCG GGCAGGGCCA CAACTCGAGT 
GGCACCCTCA GCATCCGAGA GACCCCCATA CTGGATGGGT ACGAGTATGA CATCACTGAC 
CTGCGGCATC ACCTGCAGCG GGAGTGTATG AATGGTGGAG AGGACTTCAC CAGTCAGGTC 
ACCCGCACGC TAGACTCACT TCAGGGGTGC AATGAAAAGG GGGGCATGGA CCTCACTCCA 
GGGAGTGATA GCACAAAACT GTCCCTAATG AGCAAGTACA AGGATAACAT CATTGCTACC 
AGTCCTGTCG ACAGCAACCA CCAGCAAAAC ACACTTCTTC CACACAATAC CTCCACCAGC 
CAGCGCAAAA GGCTGTCCAG CAAAACACAC GCGGGTTCAA CTTTTCTGAA CCCAGATGGA 
GACTCTGGGA CAGAGGCGGA CAGCGAGCCT CAGCTGACTT TCTACACCGA CCCAAACCGC 
AGCCGCCGAC GCAGTCGAGG TTCCCCACGG AGTCCTATCA ATAAGACCAC CCTCACTTTG 
ATCAGCGTGG TGAGCTGTGT GATCGGCCTG GTCTGTGCGT CTCACCTTTC CTGTAGCCTG 
AACGTCAGAG TGATCCTTCA CGTACCGGAG CATCTCATTG CAGACGGCTC CAGGTTTGTT 
CTGCTTCAGG GCAGTCAGCT GGATGCCTCT GATTGGCTGA ACCCTGCCCA GGTGCTGCTA 
TACTACCAGC AGAATGCCAG CGGCCCCTGG GTCAACGATC TGTGTGGCCG GCGTCTCCTG 
GACCCCTGTG AGCACCAGTG TGACCCAGAT ACAGGAGAAT GTCTCTGCTT TGATGGCTAC 
ATGAAAGACC CAGTTCACAA ACACCTTTGC ATCCGCAATG AATGGGGCCC AAATCAAGGC 
CCATGGCCCT ACACAATTTT CCAACGGGGC TTTGATTTGG TGATGGGAGA GCAGCCATCG 
GATCGCATTT TTAGATTTAC ATACACTCTT GGAGAAGGCA TGTGGCTGCC ACTTAGCAAA 
AGCTTCGTCA TTCCTCCGGC TGAGCTGGCC ATCAACCCCT CTGCCAAGTG TAAGACAGAT 
ATGACTGTCA TGGAGGACGC AGTGGATGTC AGGGAGGAAC TGATGATTTC CTCATCATTT 
GACAGTCTGG AGGTGCTGCT GGACTCATTT GGCCCTGTGA GAGACTGTTC AAAAGACAAC 
GGAGGTTGCA GCCGAAATTT CCGCTGTATT TCTGATCGCA AGCTTGACTC CACTGGCTGT 
GTGTGTCCTC CTGGATTGAG CCCGATGAAG GATGGCACCG GTTGCTATGA CCACCACATT 
GGCATTGACT GCTCGGACGG CTTTAATGGG GGCTGTGAGC AGCTGTGCCT CCAGCAGCTG 
GCACCTCTGG AGGAGGATCC CACTCTCTAC AACATCCAGA TGTTCTGCGG ATGCATTGAA 
GATTATAAGA GAGGGTCAGA TGGCCGCTCT TGTCAGCCAC TGTCTGAGGC CTGCACAGAG 
GGACTGGACT GCGGGGACAC AGCCGACATC CCAGCCAACC AGACTGTGTT TGGAGACCTC 
TTTTATGGCT ATAATAACCA CACCAAAGAG ATCACATCTG GACAGATACT AAAAGCCACT 
TTTAGGCAGA AAAACTTTGC TCGTGGCATT GAGCAGCAGC TCCCGGATGG CATGGTGGTG 
GCGTCAGTGC CGAATGAAGC CCAGTGTCAT GAGGAACTGT CTGACCCAGT GCCTGATCCA 
GAATACCTCA CTGGTATGGT AAACTACAGT GAGGTTACCG GTTACCCATT GGTTCAGCAC 
TGGAGGCTGC GCTCTGTTAT GTACCATGTA AAGCTCAATC AGTGGGCTCT TTCCCAAGCC 
TTCAGTTCTG CCATTCATTC ACTGGACGGG GCCACCCTGC GCAGCGACTT TGTGTCCATT 
TTAAAAGAGT TTGGCAACCA TTTTATCCAG GAGGCAGTGT ATGGTTTTGA GGAGTCCTGT 
ACCATATGGT ATCCCAACAA ACAAGTCCAA CGCCAGCTGT GGTTGGAGTA CCAGGATATC 
AGCAAAGGAA ACTCTCCATC GGACGAGTCG GAGGAGCGAG ACAAAGAGCC ACGCACACTT 
ACTTACCCAG CATACATTGC CAGCCTGCTG GACTCTGGTG CCAAGCGCAT GGCGGCGGGG 
GTGCGCATGG ACTGCAGCAG CCAGGGCCAG TGCCCTCTGT CCTGCCACCT CTGCCACATG 
AGCCCTGGGC CTGCACGTCC TGCAGAACCT GTGCTGCTTA ACATCACTAA AGCCACACCG 
ATCTTCGAAC TTGTCAACAG CAACGAGACG TACCAGGCTC TGCAGGAGGC CATGATGAGT 
GTTTTATGGT GCTCTGCTAA AGGGGATGTT ATTGATGACT GGTGCAGATG TGACTCCAAT 
GCTTTTGGCA CAGATGGACT GCCAACCTGT GCTCCGCTGC CTCAACCTAT GCTCAAACTG 
TCCCATTCGT ATGAGCCCAG CAGCTCATTG GTCATCATAG AATGGAATCA CACAGAGCCA 
CCAATTGGAG TGAGGATTGC TGATTACCTC ATCAGTCAGG AGAAGGTGAC AGAGCGGACT 
GATCACTCCA AAGTTGAGAC GGAGACAATG TTGAGTTTTG TGGATGACAT CATGTCAGGG 
GCCAAATCCC CGTGTGTGAT GCTTGGAGAC ATTCCCGATC CTCTGTCTTC AATCTCCTTG 
ATCATTCGCT GCCTGGAGCC GGACACCACC TACATGTTTC GGCTGTGGGC AGTGGACAAC 
ACGGGTCGGC GCTCCAGTCC GAGTGAGGTG ACGATCAAAA CTCCTTGTCC TGCTGTGGAT 
GATGTCAAGG CTCAAGAAAT AGCAGATAAG ATTTACAATC TGTTCAACGG CTACACCAGC 
GGGAAGGAGC AGCAGACGGC CTATAATACA CTCATGGATC TGGGATCACC AACCCTCCAC 
CGAGTTCTAT ACCACTACAA TCAGCGCTAC GAGAGTTTTG GAGAGTTCAC CTGGCGATGT 
GAAGATGAAC TTGGTCCAAG GAAGGCAGGA CTGATCCTGT CTCAGCTGGA TGAGCTCAGC 
GGTTGGTGTC GGGGGCTCCT CCAGGAACCA AAGATTGGCC TGCGCAGAGC ATCCCTCAAG 
TATCTGTCTT GTCGATACAC AGATACCAAA GCGTTCGGGC TCAGCTGGGT GAACCTCGGC 
CAAGACTTGC GTAAGGCCTG CGAGGAGCAG ATGCTGTCCG TGATGTACAA CGATTATGGA 
GAACCCAAGG AACTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017207386.1
CDS	329..4246
Translation	MMLPPRSCMMLPPRSYLLIGVFWIVLFSSAGVKCLNGEEDQSQDMECKMKSVTVSALPFLRENDLSIMHSPSASEPKLLFSVRNDFPGEIVVVDDLENTELPYFVLEISGNSEDIPLVRWRQQWLENGTLLFHIHHQDGSQNLPGPAATAYPASDSAEEELRILHISVMGGMIALLLSILCLVLILYTRRRWCKRRRVPQPQKSASAEAANEIHYIPSVLMGGQARESLRNSRGQGHNSSGTLSIRETPILDGYEYDITDLRHHLQRECMNGGEDFTSQVTRTLDSLQGCNEKGGMDLTPGSDSTKLSLMSKYKDNIIATSPVDSNHQQNTLLPHNTSTSQRKRLSSKTHAGSTFLNPDGDSGTEADSEPQLTFYTDPNRSRRRSRGSPRSPINKTTLTLISVVSCVIGLVCASHLSCSLNVRVILHVPEHLIADGSRFVLLQGSQLDASDWLNPAQVLLYYQQNASGPWVNDLCGRRLLDPCEHQCDPDTGECLCFDGYMKDPVHKHLCIRNEWGPNQGPWPYTIFQRGFDLVMGEQPSDRIFRFTYTLGEGMWLPLSKSFVIPPAELAINPSAKCKTDMTVMEDAVDVREELMISSSFDSLEVLLDSFGPVRDCSKDNGGCSRNFRCISDRKLDSTGCVCPPGLSPMKDGTGCYDHHIGIDCSDGFNGGCEQLCLQQLAPLEEDPTLYNIQMFCGCIEDYKRGSDGRSCQPLSEACTEGLDCGDTADIPANQTVFGDLFYGYNNHTKEITSGQILKATFRQKNFARGIEQQLPDGMVVASVPNEAQCHEELSDPVPDPEYLTGMVNYSEVTGYPLVQHWRLRSVMYHVKLNQWALSQAFSSAIHSLDGATLRSDFVSILKEFGNHFIQEAVYGFEESCTIWYPNKQVQRQLWLEYQDISKGNSPSDESEERDKEPRTLTYPAYIASLLDSGAKRMAAGVRMDCSSQGQCPLSCHLCHMSPGPARPAEPVLLNITKATPIFELVNSNETYQALQEAMMSVLWCSAKGDVIDDWCRCDSNAFGTDGLPTCAPLPQPMLKLSHSYEPSSSLVIIEWNHTEPPIGVRIADYLISQEKVTERTDHSKVETETMLSFVDDIMSGAKSPCVMLGDIPDPLSSISLIIRCLEPDTTYMFRLWAVDNTGRRSSPSEVTIKTPCPAVDDVKAQEIADKIYNLFNGYTSGKEQQTAYNTLMDLGSPTLHRVLYHYNQRYESFGEFTWRCEDELGPRKAGLILSQLDELSGWCRGLLQEPKIGLRRASLKYLSCRYTDTKAFGLSWVNLGQDLRKACEEQMLSVMYNDYGEPKEL

Target ORF information:

RefSeq Version	XM_017351897.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio astrotactin 1 (astn1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017351897.2

ORF Insert Sequence:

ATGATGCTAC CGCCGCGCTC CTGTATGATG CTACCGCCGC GCTCCTATCT GCTTATCGGC 
GTTTTTTGGA TTGTCCTCTT CAGCTCTGCT GGCGTAAAAT GTCTGAACGG CGAAGAAGAT 
CAGTCTCAAG ACATGGAGTG CAAGATGAAA AGCGTTACGG TCTCCGCGTT GCCGTTTTTG 
CGAGAGAACG ACCTGAGCAT CATGCATAGC CCGTCGGCCT CCGAGCCCAA GCTACTCTTC 
TCCGTCAGGA ATGATTTTCC CGGCGAGATA GTCGTTGTTG ACGACTTGGA AAATACCGAG 
CTCCCGTACT TTGTGTTAGA GATCTCCGGG AACTCTGAAG ACATTCCTCT GGTGCGCTGG 
CGACAGCAGT GGTTGGAAAA TGGCACTTTG CTTTTCCACA TTCACCATCA GGACGGCAGT 
CAGAACTTGC CTGGTCCGGC GGCCACTGCT TACCCTGCTA GTGACTCTGC TGAAGAGGAG 
CTGCGCATTC TGCACATTTC TGTCATGGGT GGCATGATCG CTCTTCTGCT GTCCATCCTG 
TGCTTGGTGT TGATCCTGTA CACTCGCAGA CGCTGGTGTA AACGTCGCCG GGTTCCTCAA 
CCTCAAAAGA GCGCCAGTGC TGAAGCTGCC AATGAGATCC ACTACATCCC CTCAGTACTG 
ATGGGTGGAC AGGCCCGGGA AAGTCTGAGA AATTCGCGCG GGCAGGGCCA CAACTCGAGT 
GGCACCCTCA GCATCCGAGA GACCCCCATA CTGGATGGGT ACGAGTATGA CATCACTGAC 
CTGCGGCATC ACCTGCAGCG GGAGTGTATG AATGGTGGAG AGGACTTCAC CAGTCAGGTC 
ACCCGCACGC TAGACTCACT TCAGGGGTGC AATGAAAAGG GGGGCATGGA CCTCACTCCA 
GGGAGTGATA GCACAAAACT GTCCCTAATG AGCAAGTACA AGGATAACAT CATTGCTACC 
AGTCCTGTCG ACAGCAACCA CCAGCAAAAC ACACTTCTTC CACACAATAC CTCCACCAGC 
CAGCGCAAAA GGCTGTCCAG CAAAACACAC GCGGGTTCAA CTTTTCTGAA CCCAGATGGA 
GACTCTGGGA CAGAGGCGGA CAGCGAGCCT CAGCTGACTT TCTACACCGA CCCAAACCGC 
AGCCGCCGAC GCAGTCGAGG TTCCCCACGG AGTCCTATCA ATAAGACCAC CCTCACTTTG 
ATCAGCGTGG TGAGCTGTGT GATCGGCCTG GTCTGTGCGT CTCACCTTTC CTGTAGCCTG 
AACGTCAGAG TGATCCTTCA CGTACCGGAG CATCTCATTG CAGACGGCTC CAGGTTTGTT 
CTGCTTCAGG GCAGTCAGCT GGATGCCTCT GATTGGCTGA ACCCTGCCCA GGTGCTGCTA 
TACTACCAGC AGAATGCCAG CGGCCCCTGG GTCAACGATC TGTGTGGCCG GCGTCTCCTG 
GACCCCTGTG AGCACCAGTG TGACCCAGAT ACAGGAGAAT GTCTCTGCTT TGATGGCTAC 
ATGAAAGACC CAGTTCACAA ACACCTTTGC ATCCGCAATG AATGGGGCCC AAATCAAGGC 
CCATGGCCCT ACACAATTTT CCAACGGGGC TTTGATTTGG TGATGGGAGA GCAGCCATCG 
GATCGCATTT TTAGATTTAC ATACACTCTT GGAGAAGGCA TGTGGCTGCC ACTTAGCAAA 
AGCTTCGTCA TTCCTCCGGC TGAGCTGGCC ATCAACCCCT CTGCCAAGTG TAAGACAGAT 
ATGACTGTCA TGGAGGACGC AGTGGATGTC AGGGAGGAAC TGATGATTTC CTCATCATTT 
GACAGTCTGG AGGTGCTGCT GGACTCATTT GGCCCTGTGA GAGACTGTTC AAAAGACAAC 
GGAGGTTGCA GCCGAAATTT CCGCTGTATT TCTGATCGCA AGCTTGACTC CACTGGCTGT 
GTGTGTCCTC CTGGATTGAG CCCGATGAAG GATGGCACCG GTTGCTATGA CCACCACATT 
GGCATTGACT GCTCGGACGG CTTTAATGGG GGCTGTGAGC AGCTGTGCCT CCAGCAGCTG 
GCACCTCTGG AGGAGGATCC CACTCTCTAC AACATCCAGA TGTTCTGCGG ATGCATTGAA 
GATTATAAGA GAGGGTCAGA TGGCCGCTCT TGTCAGCCAC TGTCTGAGGC CTGCACAGAG 
GGACTGGACT GCGGGGACAC AGCCGACATC CCAGCCAACC AGACTGTGTT TGGAGACCTC 
TTTTATGGCT ATAATAACCA CACCAAAGAG ATCACATCTG GACAGATACT AAAAGCCACT 
TTTAGGCAGA AAAACTTTGC TCGTGGCATT GAGCAGCAGC TCCCGGATGG CATGGTGGTG 
GCGTCAGTGC CGAATGAAGC CCAGTGTCAT GAGGAACTGT CTGACCCAGT GCCTGATCCA 
GAATACCTCA CTGGTATGGT AAACTACAGT GAGGTTACCG GTTACCCATT GGTTCAGCAC 
TGGAGGCTGC GCTCTGTTAT GTACCATGTA AAGCTCAATC AGTGGGCTCT TTCCCAAGCC 
TTCAGTTCTG CCATTCATTC ACTGGACGGG GCCACCCTGC GCAGCGACTT TGTGTCCATT 
TTAAAAGAGT TTGGCAACCA TTTTATCCAG GAGGCAGTGT ATGGTTTTGA GGAGTCCTGT 
ACCATATGGT ATCCCAACAA ACAAGTCCAA CGCCAGCTGT GGTTGGAGTA CCAGGATATC 
AGCAAAGGAA ACTCTCCATC GGACGAGTCG GAGGAGCGAG ACAAAGAGCC ACGCACACTT 
ACTTACCCAG CATACATTGC CAGCCTGCTG GACTCTGGTG CCAAGCGCAT GGCGGCGGGG 
GTGCGCATGG ACTGCAGCAG CCAGGGCCAG TGCCCTCTGT CCTGCCACCT CTGCCACATG 
AGCCCTGGGC CTGCACGTCC TGCAGAACCT GTGCTGCTTA ACATCACTAA AGCCACACCG 
ATCTTCGAAC TTGTCAACAG CAACGAGACG TACCAGGCTC TGCAGGAGGC CATGATGAGT 
GTTTTATGGT GCTCTGCTAA AGGGGATGTT ATTGATGACT GGTGCAGATG TGACTCCAAT 
GCTTTTGGCA CAGATGGACT GCCAACCTGT GCTCCGCTGC CTCAACCTAT GCTCAAACTG 
TCCCATTCGT ATGAGCCCAG CAGCTCATTG GTCATCATAG AATGGAATCA CACAGAGCCA 
CCAATTGGAG TGAGGATTGC TGATTACCTC ATCAGTCAGG AGAAGGTGAC AGAGCGGACT 
GATCACTCCA AAGTTGAGAC GGAGACAATG TTGAGTTTTG TGGATGACAT CATGTCAGGG 
GCCAAATCCC CGTGTGTGAT GCTTGGAGAC ATTCCCGATC CTCTGTCTTC AATCTCCTTG 
ATCATTCGCT GCCTGGAGCC GGACACCACC TACATGTTTC GGCTGTGGGC AGTGGACAAC 
ACGGGTCGGC GCTCCAGTCC GAGTGAGGTG ACGATCAAAA CTCCTTGTCC TGCTGTGGAT 
GATGTCAAGG CTCAAGAAAT AGCAGATAAG ATTTACAATC TGTTCAACGG CTACACCAGC 
GGGAAGGAGC AGCAGACGGC CTATAATACA CTCATGGATC TGGGATCACC AACCCTCCAC 
CGAGTTCTAT ACCACTACAA TCAGCGCTAC GAGAGTTTTG GAGAGTTCAC CTGGCGATGT 
GAAGATGAAC TTGGTCCAAG GAAGGCAGGA CTGATCCTGT CTCAGCTGGA TGAGCTCAGC 
GGTTGGTGTC GGGGGCTCCT CCAGGAACCA AAGATTGGCC TGCGCAGAGC ATCCCTCAAG 
TATCTGTCTT GTCGATACAC AGATACCAAA GCGTTCGGGC TCAGCTGGGT GAACCTCGGC 
CAAGACTTGC GTAAGGCCTG CGAGGAGCAG ATGCTGTCCG TGATGTACAA CGATTATGGA 
GAACCCAAGG AACTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

astn1 ( XM_017351899.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017207388.1 Danio rerio astrotactin 1 (astn1), transcript variant X4, mRNA.

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CloneID	ODa56455
Clone ID Related Accession (Same CDS sequence)	XM_017351899.2 , XM_017351899.1
Accession Version	XM_017351899.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3810bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	astrotactin-1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017351899.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGCTAC CGCCGCGCTC CTGTATGATG CTACCGCCGC GCTCCTATCT GCTTATCGGC 
GTTTTTTGGA TTGTCCTCTT CAGCTCTGCT GGCGTAAAAT GTCTGAACGG CGAAGAAGAT 
CAGTCTCAAG ACATGGAGTG CAAGATGAAA AGCGTTACGG TCTCCGCGTT GCCGTTTTTG 
CGAGAGAACG ACCTGAGCAT CATGCATAGC CCGTCGGCCT CCGAGCCCAA GCTACTCTTC 
TCCGTCAGGA ATGATTTTCC CGGCGAGATA GTCGTTGTTG ACGACTTGGA AAATACCGAG 
CTCCCGTACT TTGTGTTAGA GATCTCCGGG AACTCTGAAG ACATTCCTCT GGTGCGCTGG 
CGACAGCAGT GGTTGGAAAA TGGCACTTTG CTTTTCCACA TTCACCATCA GGACGGCAGT 
CAGAACTTGC CTGGTCCGGC GGCCACTGCT TACCCTGCTA GTGACTCTGC TGAAGAGGAG 
CTGCGCATTC TGCACATTTC TGTCATGGGT GGCATGATCG CTCTTCTGCT GTCCATCCTG 
TGCTTGGTGT TGATCCTGTA CACTCGCAGA CGCTGGTGTA AACGTCGCCG GGTTCCTCAA 
CCTCAAAAGA GCGCCAGTGC TGAAGCTGCC AATGAGATCC ACTACATCCC CTCAGTACTG 
ATGGGTGGAC AGGCCCGGGA AAGTCTGAGA AATTCGCGCG GGCAGGGCCA CAACTCGAGT 
GGCACCCTCA GCATCCGAGA GACCCCCATA CTGGATGGGT ACGAGTATGA CATCACTGAC 
CTGCGGCATC ACCTGCAGCG GGAGTGTATG AATGGTGGAG AGGACTTCAC CAGTCAGGTC 
ACCCGCACGC TAGACTCACT TCAGGGGTGC AATGAAAAGG GGGGCATGGA CCTCACTCCA 
GGGAGTGATA GCACAAAACT GTCCCTAATG AGCAAGTACA AGGATAACAT CATTGCTACC 
AGTCCTGTCG ACAGCAACCA CCAGCAAAAC ACACTTCTTC CACACAATAC CTCCACCAGC 
CAGCGCAAAA GGCTGTCCAG CAAAACACAC GGTTCCCCAC GGAGTCCTAT CAATAAGACC 
ACCCTCACTT TGATCAGCGT GGTGAGCTGT GTGATCGGCC TGGTCTGTGC GTCTCACCTT 
TCCTGTAGCC TGAACGTCAG AGTGATCCTT CACGTACCGG AGCATCTCAT TGCAGACGGC 
TCCAGGTTTG TTCTGCTTCA GGGCAGTCAG CTGGATGCCT CTGATTGGCT GAACCCTGCC 
CAGGTGCTGC TATACTACCA GCAGAATGCC AGCGGCCCCT GGGTCAACGA TCTGTGTGGC 
CGGCGTCTCC TGGACCCCTG TGAGCACCAG TGTGACCCAG ATACAGGAGA ATGTCTCTGC 
TTTGATGGCT ACATGAAAGA CCCAGTTCAC AAACACCTTT GCATCCGCAA TGAATGGGGC 
CCAAATCAAG GCCCATGGCC CTACACAATT TTCCAACGGG GCTTTGATTT GGTGATGGGA 
GAGCAGCCAT CGGATCGCAT TTTTAGATTT ACATACACTC TTGGAGAAGG CATGTGGCTG 
CCACTTAGCA AAAGCTTCGT CATTCCTCCG GCTGAGCTGG CCATCAACCC CTCTGCCAAG 
TGTAAGACAG ATATGACTGT CATGGAGGAC GCAGTGGATG TCAGGGAGGA ACTGATGATT 
TCCTCATCAT TTGACAGTCT GGAGGTGCTG CTGGACTCAT TTGGCCCTGT GAGAGACTGT 
TCAAAAGACA ACGGAGGTTG CAGCCGAAAT TTCCGCTGTA TTTCTGATCG CAAGCTTGAC 
TCCACTGGCT GTGTGTGTCC TCCTGGATTG AGCCCGATGA AGGATGGCAC CGGTTGCTAT 
GACCACCACA TTGGCATTGA CTGCTCGGAC GGCTTTAATG GGGGCTGTGA GCAGCTGTGC 
CTCCAGCAGC TGGCACCTCT GGAGGAGGAT CCCACTCTCT ACAACATCCA GATGTTCTGC 
GGATGCATTG AAGATTATAA GAGAGGGTCA GATGGCCGCT CTTGTCAGCC ACTGTCTGAG 
GCCTGCACAG AGGGACTGGA CTGCGGGGAC ACAGCCGACA TCCCAGCCAA CCAGACTGTG 
TTTGGAGACC TCTTTTATGG CTATAATAAC CACACCAAAG AGATCACATC TGGACAGATA 
CTAAAAGCCA CTTTTAGGCA GAAAAACTTT GCTCGTGGCA TTGAGCAGCA GCTCCCGGAT 
GGCATGGTGG TGGCGTCAGT GCCGAATGAA GCCCAGTGTC ATGAGGAACT GTCTGACCCA 
GTGCCTGATC CAGAATACCT CACTGGTATG GTAAACTACA GTGAGGTTAC CGGTTACCCA 
TTGGTTCAGC ACTGGAGGCT GCGCTCTGTT ATGTACCATG TAAAGCTCAA TCAGTGGGCT 
CTTTCCCAAG CCTTCAGTTC TGCCATTCAT TCACTGGACG GGGCCACCCT GCGCAGCGAC 
TTTGTGTCCA TTTTAAAAGA GTTTGGCAAC CATTTTATCC AGGAGGCAGT GTATGGTTTT 
GAGGAGTCCT GTACCATATG GTATCCCAAC AAACAAGTCC AACGCCAGCT GTGGTTGGAG 
TACCAGGATA TCAGCAAAGG AAACTCTCCA TCGGACGAGT CGGAGGAGCG AGACAAAGAG 
CCACGCACAC TTACTTACCC AGCATACATT GCCAGCCTGC TGGACTCTGG TGCCAAGCGC 
ATGGCGGCGG GGGTGCGCAT GGACTGCAGC AGCCAGGGCC AGTGCCCTCT GTCCTGCCAC 
CTCTGCCACA TGAGCCCTGG GCCTGCACGT CCTGCAGAAC CTGTGCTGCT TAACATCACT 
AAAGCCACAC CGATCTTCGA ACTTGTCAAC AGCAACGAGA CGTACCAGGC TCTGCAGGAG 
GCCATGATGA GTGTTTTATG GTGCTCTGCT AAAGGGGATG TTATTGATGA CTGGTGCAGA 
TGTGACTCCA ATGCTTTTGG CACAGATGGA CTGCCAACCT GTGCTCCGCT GCCTCAACCT 
ATGCTCAAAC TGTCCCATTC GTATGAGCCC AGCAGCTCAT TGGTCATCAT AGAATGGAAT 
CACACAGAGC CACCAATTGG AGTGAGGATT GCTGATTACC TCATCAGTCA GGAGAAGGTG 
ACAGAGCGGA CTGATCACTC CAAAGTTGAG ACGGAGACAA TGTTGAGTTT TGTGGATGAC 
ATCATGTCAG GGGCCAAATC CCCGTGTGTG ATGCTTGGAG ACATTCCCGA TCCTCTGTCT 
TCAATCTCCT TGATCATTCG CTGCCTGGAG CCGGACACCA CCTACATGTT TCGGCTGTGG 
GCAGTGGACA ACACGGGTCG GCGCTCCAGT CCGAGTGAGG TGACGATCAA AACTCCTTGT 
CCTGCTGTGG ATGATGTCAA GGCTCAAGAA ATAGCAGATA AGATTTACAA TCTGTTCAAC 
GGCTACACCA GCGGGAAGGA GCAGCAGACG GCCTATAATA CACTCATGGA TCTGGGATCA 
CCAACCCTCC ACCGAGTTCT ATACCACTAC AATCAGCGCT ACGAGAGTTT TGGAGAGTTC 
ACCTGGCGAT GTGAAGATGA ACTTGGTCCA AGGAAGGCAG GACTGATCCT GTCTCAGCTG 
GATGAGCTCA GCGGTTGGTG TCGGGGGCTC CTCCAGGAAC CAAAGATTGG CCTGCGCAGA 
GCATCCCTCA AGTATCTGTC TTGTCGATAC ACAGATACCA AAGCGTTCGG GCTCAGCTGG 
GTGAACCTCG GCCAAGACTT GCGTAAGGCC TGCGAGGAGC AGATGCTGTC CGTGATGTAC 
AACGATTATG GAGAACCCAA GGAACTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017207388.1
CDS	328..4137
Translation	MMLPPRSCMMLPPRSYLLIGVFWIVLFSSAGVKCLNGEEDQSQDMECKMKSVTVSALPFLRENDLSIMHSPSASEPKLLFSVRNDFPGEIVVVDDLENTELPYFVLEISGNSEDIPLVRWRQQWLENGTLLFHIHHQDGSQNLPGPAATAYPASDSAEEELRILHISVMGGMIALLLSILCLVLILYTRRRWCKRRRVPQPQKSASAEAANEIHYIPSVLMGGQARESLRNSRGQGHNSSGTLSIRETPILDGYEYDITDLRHHLQRECMNGGEDFTSQVTRTLDSLQGCNEKGGMDLTPGSDSTKLSLMSKYKDNIIATSPVDSNHQQNTLLPHNTSTSQRKRLSSKTHGSPRSPINKTTLTLISVVSCVIGLVCASHLSCSLNVRVILHVPEHLIADGSRFVLLQGSQLDASDWLNPAQVLLYYQQNASGPWVNDLCGRRLLDPCEHQCDPDTGECLCFDGYMKDPVHKHLCIRNEWGPNQGPWPYTIFQRGFDLVMGEQPSDRIFRFTYTLGEGMWLPLSKSFVIPPAELAINPSAKCKTDMTVMEDAVDVREELMISSSFDSLEVLLDSFGPVRDCSKDNGGCSRNFRCISDRKLDSTGCVCPPGLSPMKDGTGCYDHHIGIDCSDGFNGGCEQLCLQQLAPLEEDPTLYNIQMFCGCIEDYKRGSDGRSCQPLSEACTEGLDCGDTADIPANQTVFGDLFYGYNNHTKEITSGQILKATFRQKNFARGIEQQLPDGMVVASVPNEAQCHEELSDPVPDPEYLTGMVNYSEVTGYPLVQHWRLRSVMYHVKLNQWALSQAFSSAIHSLDGATLRSDFVSILKEFGNHFIQEAVYGFEESCTIWYPNKQVQRQLWLEYQDISKGNSPSDESEERDKEPRTLTYPAYIASLLDSGAKRMAAGVRMDCSSQGQCPLSCHLCHMSPGPARPAEPVLLNITKATPIFELVNSNETYQALQEAMMSVLWCSAKGDVIDDWCRCDSNAFGTDGLPTCAPLPQPMLKLSHSYEPSSSLVIIEWNHTEPPIGVRIADYLISQEKVTERTDHSKVETETMLSFVDDIMSGAKSPCVMLGDIPDPLSSISLIIRCLEPDTTYMFRLWAVDNTGRRSSPSEVTIKTPCPAVDDVKAQEIADKIYNLFNGYTSGKEQQTAYNTLMDLGSPTLHRVLYHYNQRYESFGEFTWRCEDELGPRKAGLILSQLDELSGWCRGLLQEPKIGLRRASLKYLSCRYTDTKAFGLSWVNLGQDLRKACEEQMLSVMYNDYGEPKEL

Target ORF information:

RefSeq Version	XM_017351899.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio astrotactin 1 (astn1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017351899.2

ORF Insert Sequence:

ATGATGCTAC CGCCGCGCTC CTGTATGATG CTACCGCCGC GCTCCTATCT GCTTATCGGC 
GTTTTTTGGA TTGTCCTCTT CAGCTCTGCT GGCGTAAAAT GTCTGAACGG CGAAGAAGAT 
CAGTCTCAAG ACATGGAGTG CAAGATGAAA AGCGTTACGG TCTCCGCGTT GCCGTTTTTG 
CGAGAGAACG ACCTGAGCAT CATGCATAGC CCGTCGGCCT CCGAGCCCAA GCTACTCTTC 
TCCGTCAGGA ATGATTTTCC CGGCGAGATA GTCGTTGTTG ACGACTTGGA AAATACCGAG 
CTCCCGTACT TTGTGTTAGA GATCTCCGGG AACTCTGAAG ACATTCCTCT GGTGCGCTGG 
CGACAGCAGT GGTTGGAAAA TGGCACTTTG CTTTTCCACA TTCACCATCA GGACGGCAGT 
CAGAACTTGC CTGGTCCGGC GGCCACTGCT TACCCTGCTA GTGACTCTGC TGAAGAGGAG 
CTGCGCATTC TGCACATTTC TGTCATGGGT GGCATGATCG CTCTTCTGCT GTCCATCCTG 
TGCTTGGTGT TGATCCTGTA CACTCGCAGA CGCTGGTGTA AACGTCGCCG GGTTCCTCAA 
CCTCAAAAGA GCGCCAGTGC TGAAGCTGCC AATGAGATCC ACTACATCCC CTCAGTACTG 
ATGGGTGGAC AGGCCCGGGA AAGTCTGAGA AATTCGCGCG GGCAGGGCCA CAACTCGAGT 
GGCACCCTCA GCATCCGAGA GACCCCCATA CTGGATGGGT ACGAGTATGA CATCACTGAC 
CTGCGGCATC ACCTGCAGCG GGAGTGTATG AATGGTGGAG AGGACTTCAC CAGTCAGGTC 
ACCCGCACGC TAGACTCACT TCAGGGGTGC AATGAAAAGG GGGGCATGGA CCTCACTCCA 
GGGAGTGATA GCACAAAACT GTCCCTAATG AGCAAGTACA AGGATAACAT CATTGCTACC 
AGTCCTGTCG ACAGCAACCA CCAGCAAAAC ACACTTCTTC CACACAATAC CTCCACCAGC 
CAGCGCAAAA GGCTGTCCAG CAAAACACAC GGTTCCCCAC GGAGTCCTAT CAATAAGACC 
ACCCTCACTT TGATCAGCGT GGTGAGCTGT GTGATCGGCC TGGTCTGTGC GTCTCACCTT 
TCCTGTAGCC TGAACGTCAG AGTGATCCTT CACGTACCGG AGCATCTCAT TGCAGACGGC 
TCCAGGTTTG TTCTGCTTCA GGGCAGTCAG CTGGATGCCT CTGATTGGCT GAACCCTGCC 
CAGGTGCTGC TATACTACCA GCAGAATGCC AGCGGCCCCT GGGTCAACGA TCTGTGTGGC 
CGGCGTCTCC TGGACCCCTG TGAGCACCAG TGTGACCCAG ATACAGGAGA ATGTCTCTGC 
TTTGATGGCT ACATGAAAGA CCCAGTTCAC AAACACCTTT GCATCCGCAA TGAATGGGGC 
CCAAATCAAG GCCCATGGCC CTACACAATT TTCCAACGGG GCTTTGATTT GGTGATGGGA 
GAGCAGCCAT CGGATCGCAT TTTTAGATTT ACATACACTC TTGGAGAAGG CATGTGGCTG 
CCACTTAGCA AAAGCTTCGT CATTCCTCCG GCTGAGCTGG CCATCAACCC CTCTGCCAAG 
TGTAAGACAG ATATGACTGT CATGGAGGAC GCAGTGGATG TCAGGGAGGA ACTGATGATT 
TCCTCATCAT TTGACAGTCT GGAGGTGCTG CTGGACTCAT TTGGCCCTGT GAGAGACTGT 
TCAAAAGACA ACGGAGGTTG CAGCCGAAAT TTCCGCTGTA TTTCTGATCG CAAGCTTGAC 
TCCACTGGCT GTGTGTGTCC TCCTGGATTG AGCCCGATGA AGGATGGCAC CGGTTGCTAT 
GACCACCACA TTGGCATTGA CTGCTCGGAC GGCTTTAATG GGGGCTGTGA GCAGCTGTGC 
CTCCAGCAGC TGGCACCTCT GGAGGAGGAT CCCACTCTCT ACAACATCCA GATGTTCTGC 
GGATGCATTG AAGATTATAA GAGAGGGTCA GATGGCCGCT CTTGTCAGCC ACTGTCTGAG 
GCCTGCACAG AGGGACTGGA CTGCGGGGAC ACAGCCGACA TCCCAGCCAA CCAGACTGTG 
TTTGGAGACC TCTTTTATGG CTATAATAAC CACACCAAAG AGATCACATC TGGACAGATA 
CTAAAAGCCA CTTTTAGGCA GAAAAACTTT GCTCGTGGCA TTGAGCAGCA GCTCCCGGAT 
GGCATGGTGG TGGCGTCAGT GCCGAATGAA GCCCAGTGTC ATGAGGAACT GTCTGACCCA 
GTGCCTGATC CAGAATACCT CACTGGTATG GTAAACTACA GTGAGGTTAC CGGTTACCCA 
TTGGTTCAGC ACTGGAGGCT GCGCTCTGTT ATGTACCATG TAAAGCTCAA TCAGTGGGCT 
CTTTCCCAAG CCTTCAGTTC TGCCATTCAT TCACTGGACG GGGCCACCCT GCGCAGCGAC 
TTTGTGTCCA TTTTAAAAGA GTTTGGCAAC CATTTTATCC AGGAGGCAGT GTATGGTTTT 
GAGGAGTCCT GTACCATATG GTATCCCAAC AAACAAGTCC AACGCCAGCT GTGGTTGGAG 
TACCAGGATA TCAGCAAAGG AAACTCTCCA TCGGACGAGT CGGAGGAGCG AGACAAAGAG 
CCACGCACAC TTACTTACCC AGCATACATT GCCAGCCTGC TGGACTCTGG TGCCAAGCGC 
ATGGCGGCGG GGGTGCGCAT GGACTGCAGC AGCCAGGGCC AGTGCCCTCT GTCCTGCCAC 
CTCTGCCACA TGAGCCCTGG GCCTGCACGT CCTGCAGAAC CTGTGCTGCT TAACATCACT 
AAAGCCACAC CGATCTTCGA ACTTGTCAAC AGCAACGAGA CGTACCAGGC TCTGCAGGAG 
GCCATGATGA GTGTTTTATG GTGCTCTGCT AAAGGGGATG TTATTGATGA CTGGTGCAGA 
TGTGACTCCA ATGCTTTTGG CACAGATGGA CTGCCAACCT GTGCTCCGCT GCCTCAACCT 
ATGCTCAAAC TGTCCCATTC GTATGAGCCC AGCAGCTCAT TGGTCATCAT AGAATGGAAT 
CACACAGAGC CACCAATTGG AGTGAGGATT GCTGATTACC TCATCAGTCA GGAGAAGGTG 
ACAGAGCGGA CTGATCACTC CAAAGTTGAG ACGGAGACAA TGTTGAGTTT TGTGGATGAC 
ATCATGTCAG GGGCCAAATC CCCGTGTGTG ATGCTTGGAG ACATTCCCGA TCCTCTGTCT 
TCAATCTCCT TGATCATTCG CTGCCTGGAG CCGGACACCA CCTACATGTT TCGGCTGTGG 
GCAGTGGACA ACACGGGTCG GCGCTCCAGT CCGAGTGAGG TGACGATCAA AACTCCTTGT 
CCTGCTGTGG ATGATGTCAA GGCTCAAGAA ATAGCAGATA AGATTTACAA TCTGTTCAAC 
GGCTACACCA GCGGGAAGGA GCAGCAGACG GCCTATAATA CACTCATGGA TCTGGGATCA 
CCAACCCTCC ACCGAGTTCT ATACCACTAC AATCAGCGCT ACGAGAGTTT TGGAGAGTTC 
ACCTGGCGAT GTGAAGATGA ACTTGGTCCA AGGAAGGCAG GACTGATCCT GTCTCAGCTG 
GATGAGCTCA GCGGTTGGTG TCGGGGGCTC CTCCAGGAAC CAAAGATTGG CCTGCGCAGA 
GCATCCCTCA AGTATCTGTC TTGTCGATAC ACAGATACCA AAGCGTTCGG GCTCAGCTGG 
GTGAACCTCG GCCAAGACTT GCGTAAGGCC TGCGAGGAGC AGATGCTGTC CGTGATGTAC 
AACGATTATG GAGAACCCAA GGAACTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

astn1 ( XM_017351899.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017207388.1 Danio rerio astrotactin 1 (astn1), transcript variant X3, mRNA.

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CloneID	ODa56455
Clone ID Related Accession (Same CDS sequence)	XM_017351899.2 , XM_017351899.1
Accession Version	XM_017351899.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3810bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	astrotactin-1 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGCTAC CGCCGCGCTC CTGTATGATG CTACCGCCGC GCTCCTATCT GCTTATCGGC 
GTTTTTTGGA TTGTCCTCTT CAGCTCTGCT GGCGTAAAAT GTCTGAACGG CGAAGAAGAT 
CAGTCTCAAG ACATGGAGTG CAAGATGAAA AGCGTTACGG TCTCCGCGTT GCCGTTTTTG 
CGAGAGAACG ACCTGAGCAT CATGCATAGC CCGTCGGCCT CCGAGCCCAA GCTACTCTTC 
TCCGTCAGGA ATGATTTTCC CGGCGAGATA GTCGTTGTTG ACGACTTGGA AAATACCGAG 
CTCCCGTACT TTGTGTTAGA GATCTCCGGG AACTCTGAAG ACATTCCTCT GGTGCGCTGG 
CGACAGCAGT GGTTGGAAAA TGGCACTTTG CTTTTCCACA TTCACCATCA GGACGGCAGT 
CAGAACTTGC CTGGTCCGGC GGCCACTGCT TACCCTGCTA GTGACTCTGC TGAAGAGGAG 
CTGCGCATTC TGCACATTTC TGTCATGGGT GGCATGATCG CTCTTCTGCT GTCCATCCTG 
TGCTTGGTGT TGATCCTGTA CACTCGCAGA CGCTGGTGTA AACGTCGCCG GGTTCCTCAA 
CCTCAAAAGA GCGCCAGTGC TGAAGCTGCC AATGAGATCC ACTACATCCC CTCAGTACTG 
ATGGGTGGAC AGGCCCGGGA AAGTCTGAGA AATTCGCGCG GGCAGGGCCA CAACTCGAGT 
GGCACCCTCA GCATCCGAGA GACCCCCATA CTGGATGGGT ACGAGTATGA CATCACTGAC 
CTGCGGCATC ACCTGCAGCG GGAGTGTATG AATGGTGGAG AGGACTTCAC CAGTCAGGTC 
ACCCGCACGC TAGACTCACT TCAGGGGTGC AATGAAAAGG GGGGCATGGA CCTCACTCCA 
GGGAGTGATA GCACAAAACT GTCCCTAATG AGCAAGTACA AGGATAACAT CATTGCTACC 
AGTCCTGTCG ACAGCAACCA CCAGCAAAAC ACACTTCTTC CACACAATAC CTCCACCAGC 
CAGCGCAAAA GGCTGTCCAG CAAAACACAC GGTTCCCCAC GGAGTCCTAT CAATAAGACC 
ACCCTCACTT TGATCAGCGT GGTGAGCTGT GTGATCGGCC TGGTCTGTGC GTCTCACCTT 
TCCTGTAGCC TGAACGTCAG AGTGATCCTT CACGTACCGG AGCATCTCAT TGCAGACGGC 
TCCAGGTTTG TTCTGCTTCA GGGCAGTCAG CTGGATGCCT CTGATTGGCT GAACCCTGCC 
CAGGTGCTGC TATACTACCA GCAGAATGCC AGCGGCCCCT GGGTCAACGA TCTGTGTGGC 
CGGCGTCTCC TGGACCCCTG TGAGCACCAG TGTGACCCAG ATACAGGAGA ATGTCTCTGC 
TTTGATGGCT ACATGAAAGA CCCAGTTCAC AAACACCTTT GCATCCGCAA TGAATGGGGC 
CCAAATCAAG GCCCATGGCC CTACACAATT TTCCAACGGG GCTTTGATTT GGTGATGGGA 
GAGCAGCCAT CGGATCGCAT TTTTAGATTT ACATACACTC TTGGAGAAGG CATGTGGCTG 
CCACTTAGCA AAAGCTTCGT CATTCCTCCG GCTGAGCTGG CCATCAACCC CTCTGCCAAG 
TGTAAGACAG ATATGACTGT CATGGAGGAC GCAGTGGATG TCAGGGAGGA ACTGATGATT 
TCCTCATCAT TTGACAGTCT GGAGGTGCTG CTGGACTCAT TTGGCCCTGT GAGAGACTGT 
TCAAAAGACA ACGGAGGTTG CAGCCGAAAT TTCCGCTGTA TTTCTGATCG CAAGCTTGAC 
TCCACTGGCT GTGTGTGTCC TCCTGGATTG AGCCCGATGA AGGATGGCAC CGGTTGCTAT 
GACCACCACA TTGGCATTGA CTGCTCGGAC GGCTTTAATG GGGGCTGTGA GCAGCTGTGC 
CTCCAGCAGC TGGCACCTCT GGAGGAGGAT CCCACTCTCT ACAACATCCA GATGTTCTGC 
GGATGCATTG AAGATTATAA GAGAGGGTCA GATGGCCGCT CTTGTCAGCC ACTGTCTGAG 
GCCTGCACAG AGGGACTGGA CTGCGGGGAC ACAGCCGACA TCCCAGCCAA CCAGACTGTG 
TTTGGAGACC TCTTTTATGG CTATAATAAC CACACCAAAG AGATCACATC TGGACAGATA 
CTAAAAGCCA CTTTTAGGCA GAAAAACTTT GCTCGTGGCA TTGAGCAGCA GCTCCCGGAT 
GGCATGGTGG TGGCGTCAGT GCCGAATGAA GCCCAGTGTC ATGAGGAACT GTCTGACCCA 
GTGCCTGATC CAGAATACCT CACTGGTATG GTAAACTACA GTGAGGTTAC CGGTTACCCA 
TTGGTTCAGC ACTGGAGGCT GCGCTCTGTT ATGTACCATG TAAAGCTCAA TCAGTGGGCT 
CTTTCCCAAG CCTTCAGTTC TGCCATTCAT TCACTGGACG GGGCCACCCT GCGCAGCGAC 
TTTGTGTCCA TTTTAAAAGA GTTTGGCAAC CATTTTATCC AGGAGGCAGT GTATGGTTTT 
GAGGAGTCCT GTACCATATG GTATCCCAAC AAACAAGTCC AACGCCAGCT GTGGTTGGAG 
TACCAGGATA TCAGCAAAGG AAACTCTCCA TCGGACGAGT CGGAGGAGCG AGACAAAGAG 
CCACGCACAC TTACTTACCC AGCATACATT GCCAGCCTGC TGGACTCTGG TGCCAAGCGC 
ATGGCGGCGG GGGTGCGCAT GGACTGCAGC AGCCAGGGCC AGTGCCCTCT GTCCTGCCAC 
CTCTGCCACA TGAGCCCTGG GCCTGCACGT CCTGCAGAAC CTGTGCTGCT TAACATCACT 
AAAGCCACAC CGATCTTCGA ACTTGTCAAC AGCAACGAGA CGTACCAGGC TCTGCAGGAG 
GCCATGATGA GTGTTTTATG GTGCTCTGCT AAAGGGGATG TTATTGATGA CTGGTGCAGA 
TGTGACTCCA ATGCTTTTGG CACAGATGGA CTGCCAACCT GTGCTCCGCT GCCTCAACCT 
ATGCTCAAAC TGTCCCATTC GTATGAGCCC AGCAGCTCAT TGGTCATCAT AGAATGGAAT 
CACACAGAGC CACCAATTGG AGTGAGGATT GCTGATTACC TCATCAGTCA GGAGAAGGTG 
ACAGAGCGGA CTGATCACTC CAAAGTTGAG ACGGAGACAA TGTTGAGTTT TGTGGATGAC 
ATCATGTCAG GGGCCAAATC CCCGTGTGTG ATGCTTGGAG ACATTCCCGA TCCTCTGTCT 
TCAATCTCCT TGATCATTCG CTGCCTGGAG CCGGACACCA CCTACATGTT TCGGCTGTGG 
GCAGTGGACA ACACGGGTCG GCGCTCCAGT CCGAGTGAGG TGACGATCAA AACTCCTTGT 
CCTGCTGTGG ATGATGTCAA GGCTCAAGAA ATAGCAGATA AGATTTACAA TCTGTTCAAC 
GGCTACACCA GCGGGAAGGA GCAGCAGACG GCCTATAATA CACTCATGGA TCTGGGATCA 
CCAACCCTCC ACCGAGTTCT ATACCACTAC AATCAGCGCT ACGAGAGTTT TGGAGAGTTC 
ACCTGGCGAT GTGAAGATGA ACTTGGTCCA AGGAAGGCAG GACTGATCCT GTCTCAGCTG 
GATGAGCTCA GCGGTTGGTG TCGGGGGCTC CTCCAGGAAC CAAAGATTGG CCTGCGCAGA 
GCATCCCTCA AGTATCTGTC TTGTCGATAC ACAGATACCA AAGCGTTCGG GCTCAGCTGG 
GTGAACCTCG GCCAAGACTT GCGTAAGGCC TGCGAGGAGC AGATGCTGTC CGTGATGTAC 
AACGATTATG GAGAACCCAA GGAACTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017207388.1
CDS	366..4175
Translation	MMLPPRSCMMLPPRSYLLIGVFWIVLFSSAGVKCLNGEEDQSQDMECKMKSVTVSALPFLRENDLSIMHSPSASEPKLLFSVRNDFPGEIVVVDDLENTELPYFVLEISGNSEDIPLVRWRQQWLENGTLLFHIHHQDGSQNLPGPAATAYPASDSAEEELRILHISVMGGMIALLLSILCLVLILYTRRRWCKRRRVPQPQKSASAEAANEIHYIPSVLMGGQARESLRNSRGQGHNSSGTLSIRETPILDGYEYDITDLRHHLQRECMNGGEDFTSQVTRTLDSLQGCNEKGGMDLTPGSDSTKLSLMSKYKDNIIATSPVDSNHQQNTLLPHNTSTSQRKRLSSKTHGSPRSPINKTTLTLISVVSCVIGLVCASHLSCSLNVRVILHVPEHLIADGSRFVLLQGSQLDASDWLNPAQVLLYYQQNASGPWVNDLCGRRLLDPCEHQCDPDTGECLCFDGYMKDPVHKHLCIRNEWGPNQGPWPYTIFQRGFDLVMGEQPSDRIFRFTYTLGEGMWLPLSKSFVIPPAELAINPSAKCKTDMTVMEDAVDVREELMISSSFDSLEVLLDSFGPVRDCSKDNGGCSRNFRCISDRKLDSTGCVCPPGLSPMKDGTGCYDHHIGIDCSDGFNGGCEQLCLQQLAPLEEDPTLYNIQMFCGCIEDYKRGSDGRSCQPLSEACTEGLDCGDTADIPANQTVFGDLFYGYNNHTKEITSGQILKATFRQKNFARGIEQQLPDGMVVASVPNEAQCHEELSDPVPDPEYLTGMVNYSEVTGYPLVQHWRLRSVMYHVKLNQWALSQAFSSAIHSLDGATLRSDFVSILKEFGNHFIQEAVYGFEESCTIWYPNKQVQRQLWLEYQDISKGNSPSDESEERDKEPRTLTYPAYIASLLDSGAKRMAAGVRMDCSSQGQCPLSCHLCHMSPGPARPAEPVLLNITKATPIFELVNSNETYQALQEAMMSVLWCSAKGDVIDDWCRCDSNAFGTDGLPTCAPLPQPMLKLSHSYEPSSSLVIIEWNHTEPPIGVRIADYLISQEKVTERTDHSKVETETMLSFVDDIMSGAKSPCVMLGDIPDPLSSISLIIRCLEPDTTYMFRLWAVDNTGRRSSPSEVTIKTPCPAVDDVKAQEIADKIYNLFNGYTSGKEQQTAYNTLMDLGSPTLHRVLYHYNQRYESFGEFTWRCEDELGPRKAGLILSQLDELSGWCRGLLQEPKIGLRRASLKYLSCRYTDTKAFGLSWVNLGQDLRKACEEQMLSVMYNDYGEPKEL

Target ORF information:

RefSeq Version	XM_017351899.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio astrotactin 1 (astn1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017351899.1

ORF Insert Sequence:

ATGATGCTAC CGCCGCGCTC CTGTATGATG CTACCGCCGC GCTCCTATCT GCTTATCGGC 
GTTTTTTGGA TTGTCCTCTT CAGCTCTGCT GGCGTAAAAT GTCTGAACGG CGAAGAAGAT 
CAGTCTCAAG ACATGGAGTG CAAGATGAAA AGCGTTACGG TCTCCGCGTT GCCGTTTTTG 
CGAGAGAACG ACCTGAGCAT CATGCATAGC CCGTCGGCCT CCGAGCCCAA GCTACTCTTC 
TCCGTCAGGA ATGATTTTCC CGGCGAGATA GTCGTTGTTG ACGACTTGGA AAATACCGAG 
CTCCCGTACT TTGTGTTAGA GATCTCCGGG AACTCTGAAG ACATTCCTCT GGTGCGCTGG 
CGACAGCAGT GGTTGGAAAA TGGCACTTTG CTTTTCCACA TTCACCATCA GGACGGCAGT 
CAGAACTTGC CTGGTCCGGC GGCCACTGCT TACCCTGCTA GTGACTCTGC TGAAGAGGAG 
CTGCGCATTC TGCACATTTC TGTCATGGGT GGCATGATCG CTCTTCTGCT GTCCATCCTG 
TGCTTGGTGT TGATCCTGTA CACTCGCAGA CGCTGGTGTA AACGTCGCCG GGTTCCTCAA 
CCTCAAAAGA GCGCCAGTGC TGAAGCTGCC AATGAGATCC ACTACATCCC CTCAGTACTG 
ATGGGTGGAC AGGCCCGGGA AAGTCTGAGA AATTCGCGCG GGCAGGGCCA CAACTCGAGT 
GGCACCCTCA GCATCCGAGA GACCCCCATA CTGGATGGGT ACGAGTATGA CATCACTGAC 
CTGCGGCATC ACCTGCAGCG GGAGTGTATG AATGGTGGAG AGGACTTCAC CAGTCAGGTC 
ACCCGCACGC TAGACTCACT TCAGGGGTGC AATGAAAAGG GGGGCATGGA CCTCACTCCA 
GGGAGTGATA GCACAAAACT GTCCCTAATG AGCAAGTACA AGGATAACAT CATTGCTACC 
AGTCCTGTCG ACAGCAACCA CCAGCAAAAC ACACTTCTTC CACACAATAC CTCCACCAGC 
CAGCGCAAAA GGCTGTCCAG CAAAACACAC GGTTCCCCAC GGAGTCCTAT CAATAAGACC 
ACCCTCACTT TGATCAGCGT GGTGAGCTGT GTGATCGGCC TGGTCTGTGC GTCTCACCTT 
TCCTGTAGCC TGAACGTCAG AGTGATCCTT CACGTACCGG AGCATCTCAT TGCAGACGGC 
TCCAGGTTTG TTCTGCTTCA GGGCAGTCAG CTGGATGCCT CTGATTGGCT GAACCCTGCC 
CAGGTGCTGC TATACTACCA GCAGAATGCC AGCGGCCCCT GGGTCAACGA TCTGTGTGGC 
CGGCGTCTCC TGGACCCCTG TGAGCACCAG TGTGACCCAG ATACAGGAGA ATGTCTCTGC 
TTTGATGGCT ACATGAAAGA CCCAGTTCAC AAACACCTTT GCATCCGCAA TGAATGGGGC 
CCAAATCAAG GCCCATGGCC CTACACAATT TTCCAACGGG GCTTTGATTT GGTGATGGGA 
GAGCAGCCAT CGGATCGCAT TTTTAGATTT ACATACACTC TTGGAGAAGG CATGTGGCTG 
CCACTTAGCA AAAGCTTCGT CATTCCTCCG GCTGAGCTGG CCATCAACCC CTCTGCCAAG 
TGTAAGACAG ATATGACTGT CATGGAGGAC GCAGTGGATG TCAGGGAGGA ACTGATGATT 
TCCTCATCAT TTGACAGTCT GGAGGTGCTG CTGGACTCAT TTGGCCCTGT GAGAGACTGT 
TCAAAAGACA ACGGAGGTTG CAGCCGAAAT TTCCGCTGTA TTTCTGATCG CAAGCTTGAC 
TCCACTGGCT GTGTGTGTCC TCCTGGATTG AGCCCGATGA AGGATGGCAC CGGTTGCTAT 
GACCACCACA TTGGCATTGA CTGCTCGGAC GGCTTTAATG GGGGCTGTGA GCAGCTGTGC 
CTCCAGCAGC TGGCACCTCT GGAGGAGGAT CCCACTCTCT ACAACATCCA GATGTTCTGC 
GGATGCATTG AAGATTATAA GAGAGGGTCA GATGGCCGCT CTTGTCAGCC ACTGTCTGAG 
GCCTGCACAG AGGGACTGGA CTGCGGGGAC ACAGCCGACA TCCCAGCCAA CCAGACTGTG 
TTTGGAGACC TCTTTTATGG CTATAATAAC CACACCAAAG AGATCACATC TGGACAGATA 
CTAAAAGCCA CTTTTAGGCA GAAAAACTTT GCTCGTGGCA TTGAGCAGCA GCTCCCGGAT 
GGCATGGTGG TGGCGTCAGT GCCGAATGAA GCCCAGTGTC ATGAGGAACT GTCTGACCCA 
GTGCCTGATC CAGAATACCT CACTGGTATG GTAAACTACA GTGAGGTTAC CGGTTACCCA 
TTGGTTCAGC ACTGGAGGCT GCGCTCTGTT ATGTACCATG TAAAGCTCAA TCAGTGGGCT 
CTTTCCCAAG CCTTCAGTTC TGCCATTCAT TCACTGGACG GGGCCACCCT GCGCAGCGAC 
TTTGTGTCCA TTTTAAAAGA GTTTGGCAAC CATTTTATCC AGGAGGCAGT GTATGGTTTT 
GAGGAGTCCT GTACCATATG GTATCCCAAC AAACAAGTCC AACGCCAGCT GTGGTTGGAG 
TACCAGGATA TCAGCAAAGG AAACTCTCCA TCGGACGAGT CGGAGGAGCG AGACAAAGAG 
CCACGCACAC TTACTTACCC AGCATACATT GCCAGCCTGC TGGACTCTGG TGCCAAGCGC 
ATGGCGGCGG GGGTGCGCAT GGACTGCAGC AGCCAGGGCC AGTGCCCTCT GTCCTGCCAC 
CTCTGCCACA TGAGCCCTGG GCCTGCACGT CCTGCAGAAC CTGTGCTGCT TAACATCACT 
AAAGCCACAC CGATCTTCGA ACTTGTCAAC AGCAACGAGA CGTACCAGGC TCTGCAGGAG 
GCCATGATGA GTGTTTTATG GTGCTCTGCT AAAGGGGATG TTATTGATGA CTGGTGCAGA 
TGTGACTCCA ATGCTTTTGG CACAGATGGA CTGCCAACCT GTGCTCCGCT GCCTCAACCT 
ATGCTCAAAC TGTCCCATTC GTATGAGCCC AGCAGCTCAT TGGTCATCAT AGAATGGAAT 
CACACAGAGC CACCAATTGG AGTGAGGATT GCTGATTACC TCATCAGTCA GGAGAAGGTG 
ACAGAGCGGA CTGATCACTC CAAAGTTGAG ACGGAGACAA TGTTGAGTTT TGTGGATGAC 
ATCATGTCAG GGGCCAAATC CCCGTGTGTG ATGCTTGGAG ACATTCCCGA TCCTCTGTCT 
TCAATCTCCT TGATCATTCG CTGCCTGGAG CCGGACACCA CCTACATGTT TCGGCTGTGG 
GCAGTGGACA ACACGGGTCG GCGCTCCAGT CCGAGTGAGG TGACGATCAA AACTCCTTGT 
CCTGCTGTGG ATGATGTCAA GGCTCAAGAA ATAGCAGATA AGATTTACAA TCTGTTCAAC 
GGCTACACCA GCGGGAAGGA GCAGCAGACG GCCTATAATA CACTCATGGA TCTGGGATCA 
CCAACCCTCC ACCGAGTTCT ATACCACTAC AATCAGCGCT ACGAGAGTTT TGGAGAGTTC 
ACCTGGCGAT GTGAAGATGA ACTTGGTCCA AGGAAGGCAG GACTGATCCT GTCTCAGCTG 
GATGAGCTCA GCGGTTGGTG TCGGGGGCTC CTCCAGGAAC CAAAGATTGG CCTGCGCAGA 
GCATCCCTCA AGTATCTGTC TTGTCGATAC ACAGATACCA AAGCGTTCGG GCTCAGCTGG 
GTGAACCTCG GCCAAGACTT GCGTAAGGCC TGCGAGGAGC AGATGCTGTC CGTGATGTAC 
AACGATTATG GAGAACCCAA GGAACTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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astn1 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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FAX

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