tbck cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	tbck
Entrez Gene ID	791155
Full Name	TBC1 domain containing kinase
Synonyms	im:7138354,wu:fc74a10,zgc:158710
General protein information	Preferred Names TBC1 domain containing kinase Names TBC domain-containing protein kinase-like protein
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001080637.2	NP_001074106.2	TBC domain-containing protein kinase-like protein
XM_005160101.3	XP_005160158.1	TBC domain-containing protein kinase-like protein isoform X1
XM_005160101.4	XP_005160158.1	TBC domain-containing protein kinase-like protein isoform X1

Pathways from BioSystems

Homologies

	TBCK	XP_002804222.1
Mus musculus (house mouse)	Tbck	NP_001156927.1
Danio rerio (zebrafish)	tbck	NP_001074106.2
Homo sapiens (human)	TBCK	NP_001156908.1
Pan troglodytes (chimpanzee)	TBCK	XP_001170662.2
Canis lupus familiaris (dog)	TBCK	XP_545015.2
Gallus gallus (chicken)	TBCK	NP_001012595.1
Bos taurus (cattle)	TBCK	XP_002688132.1
Caenorhabditis elegans (roundworm)	tbck-1	NP_494866.2
Drosophila melanogaster (fruit fly)	CG4041	NP_572197.4
Xenopus tropicalis (tropical clawed frog)	tbck	NP_001096213.1
Rattus norvegicus (Norway rat)	Tbck	NP_001127985.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000552	XP_310531.5

Gene Ontology
Bibliography

Related articles in PubMed

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium.
Gaudet P, Livstone MS, Lewis SE, Thomas PD
Briefings in bioinformatics12(5)449-62(2011 Sep)

Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.
Strausberg RL, Feingold EA, Grouse LH, Derge JG, Klausner RD, Collins FS, Wagner L, Shenmen CM, Schuler GD, Altschul SF, Zeeberg B, Buetow KH, Schaefer CF, Bhat NK, Hopkins RF, Jordan H, Moore T, Max SI, Wang J, Hsieh F, Diatchenko L, Marusina K, Farmer AA, Rubin GM, Hong L, Stapleton M, Soares MB, Bonaldo MF, Casavant TL, Scheetz TE, Brownstein MJ, Usdin TB, Toshiyuki S, Carninci P, Prange C, Raha SS, Loquellano NA, Peters GJ, Abramson RD, Mullahy SJ, Bosak SA, McEwan PJ, McKernan KJ, Malek JA, Gunaratne PH, Richards S, Worley KC, Hale S, Garcia AM, Gay LJ, Hulyk SW, Villalon DK, Muzny DM, Sodergren EJ, Lu X, Gibbs RA, Fahey J, Helton E, Ketteman M, Madan A, Rodrigues S, Sanchez A, Whiting M, Madan A, Young AC, Shevchenko Y, Bouffard GG, Blakesley RW, Touchman JW, Green ED, Dickson MC, Rodriguez AC, Grimwood J, Schmutz J, Myers RM, Butterfield YS, Krzywinski MI, Skalska U, Smailus DE, Schnerch A, Schein JE, Jones SJ, Marra MA,
Proceedings of the National Academy of Sciences of the United States of America99(26)16899-903(2002 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following tbck gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the tbck cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa06179	NM_001080637.2 Latest version!	Danio rerio TBC1 domain containing kinase (tbck), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
ODa06179	XM_005160101.4 Latest version!	Danio rerio TBC1 domain containing kinase (tbck), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
View Old Accession Versions >>
ODa06179	XM_005160101.3	Danio rerio TBC1 domain containing kinase (tbck), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

tbck ( NM_001080637.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001074106.2 Danio rerio TBC1 domain containing kinase (tbck), mRNA.

Price & Availability↑

CloneID	ODa06179
Clone ID Related Accession (Same CDS sequence)	NM_001080637.2 , XM_005160101.3 , XM_005160101.4
Accession Version	NM_001080637.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2682bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	TBC domain-containing protein kinase-like protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CR854985.13, CR387924.6 and BX537118.12. On May 7, 2009 this sequence version replaced NM_001080637.1. ##Evidence-Data-START## CDS exon combination :: GDQH01002152.1, GDQQ01004662.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGCAGCCCC TCAGAGATGC CGAGTTGGGG GCTTCCACCT TCTTCGCTTC TCCTCTTCCT 
CACGATGTGT GCGGCAGCAA TGGGCTTCCT CTCACCCCAA ACTCCATCAA GATCCTGGGA 
CGCTTCCAGA TCCTCAAAAC CATCACCCAT CCCAGACTGT GCCAATATGT GGACATCAGC 
AGAGGAAAGC ACGAGCGCCT GGTTATTGTA GCTGAGCACT ATGAAAAAAG CTTGAAGGAC 
CTTTTGAAGC AGCGGAAACC TGTCAGTCCA GAGAAGGTGC TACAGGTTGC CTATGAAGTG 
CTGGAAGGAT TGGAGTTCAT GAATAAGCAC GGGATGGTGC ATCGCGCTTT ATCTCCTAAC 
AATGTCCTCC TAGATCGTGC GGATAAAGTG AAGCTGGCCA AATTTGGCCT GTATCATATG 
ACCGACCATG GAGCAGATGT CGACTTCCCA ATTGGGTATC CATCTTACCT GCCTCCGGAG 
GTTATTGCTC AGGGCTGTTT CTATTCCAGC GAGCCCTCCA TCAATGAGGC CCCGCAGCCT 
TCAGGGCCCA AAACTGACGT CTGGTCTCTC GGGGTGCTTC TGTTTGAACT CTGTGTGGGA 
AGGCAGATTC TGCAGAACAT AGACATCAGC GAGAGAATGA AGTTCATCAT CACCTTAGGG 
TGCATGGATG ACATCATTAC AGTATTAGCC GAGGAACACG GGTGCATAGA CACAATTAAG 
GATCTGCCTG AAAACGTGCA GGCTTTATTG AGGAAATGCC TGACGTTTCT CCCTTCAAAA 
CGACCCACTC CTTCTGATCT TCTGGGAGAC GCAGTGTTTG AGAGCATATC TTCTGAGTAT 
ACTCCCTATC AGAGTCCTGT GCATCTGTTC TCCGCCTCAC CACGATGTGC TTATCTACAG 
CTTCCTGATG ACATCAGTGA ACTCTGTAGA GAGAATGGAG AGAACTACCT GTCGGAGAGA 
GCCATAGATG AGGTTTATCA CCTGTGGTGT CTGGCAGGGG GAGACCTGGA GAAAGAGCTC 
ACCAATCAGG AGATCATCCA GTCCAAACCA CCCATCTGCA CGCTTCCCAA GTTTATTTTG 
GAGGATGGCG AGTCGTTTGG ACAGGGCCGG GACAGAAGCT TCCTCCTGGA TGACACAACT 
GTGACACTTT CTCTCTTTCA GCTCAGAAAC AGGCTGAAGG ATGTGGCGGG AGAGACATAC 
TTTCCATTAT TGGAGGATGA GCAGTGCAGC CTGCCTCAGT CCAGCAGCAG TAATGAACTG 
TCTGCCACCG TCACACTGCC CCTCATTATC CGAGAGAGAG ACACCGAGTA CCAGCTCACA 
CGCATCGTCC TCTTCCACAG ACTGCTCAAG GCATACCCTT ACAAGAAGAA CCAGATCTGG 
AAGGAGGCGA GGGTTGACAT TCCTCCTCTT CTGAGGGGTC TGGCCTGGGC GGCTTTACTC 
GGAGTTGAGG GCGACATCCA ATCAAAATAC GACTCCATCG ATAAGGACAC GCCCATCCCC 
ACTGACAGAC AGATTGAAGT GGATATTCCT CGCTGTCACC AATATGATGA GCTGCTGTCG 
TCGCCGCAGG GCCATGTCAA GTTTCGGCGG GTGCTGAAGG CTTGGGTTGT GTCTCATCCC 
GATCTGGTTT ACTGGCAAGG CTTGGATTCA CTTTGTGCAC CTTTCCTTTA TTTAAATTTC 
AACAATGAAG CTCTTGCGTA CGCATGCATG TCGGCCTTCA TCCCCAAATA TCTGTACAAT 
TTCTTCCTGA AGGACAACTC GCATGTAATA CAAGAATATC TGACCGTCTT CTCTCAGATG 
ATTGCGTTTC ACGACCCCGA GCTCAGCAAC CACCTCAACG AGATTGGATT CATACCTGAT 
CTCTACGCAA TCCCATGGTT TCTTACAATG TTCACACACG TGTTTCCCCT GCACAAGATC 
TTCCACCTGT GGGACACTCT GCTCTTGGGA AACTCCTCGT TCCCCTTCTG CATTGGTGTA 
GCCATCTTAC AGCAGCTGAG AGACCGTCTG CTGGCCAACG GCTTCAACGA GTGCATACTG 
CTCTTCTCTG ACCTCCCTGA GATCGACATT GAACGCTGTG TTCGAGAATC CATCAACCTT 
TTTTGCTGGA CTCCTAAAAG TGCCACTTAC CGCCAGCATG CCCAGCCAAA CAAACCTCCC 
AGCGAGAGCA ACAGCTTCGG CAAAACACCT TCATATTACG CATCAGAGTT TCAAGAACTG 
ACCAAAACTG ACCTGAGCCG TGAGCCACTC AAGCTGAGCG AGCTGAAAGC AGAAGTGTCT 
CCGCGTATTT CTGTGGAAGA CCTCATTGAC CTGTGTGAGC TGAGTGGACC AGCTTTCTTT 
AAAACTCCCG TCAAGAGGGC CAGGACTGGC AAACCCAAAA TTCTTGCGGT GGACATCAGG 
AGCCTTGAAG ACTTCAGTCG AGGCCACATC TACGGCAGCA TTAATATTCC ATACACCTCC 
ACGTTTGGAC CAGAGGGAGA GCTGCTCCAG TGTCCCGCTA GCAGCAGCCT AGCCGGATAC 
AGACTCCGCG TCATCGTCAT CCTCAGCAGC GTCATGAAGA ACGCTATCAC TTTTGCGACT 
CATCTGGTGA AGGTGAATTT CCCTCGTGTT TGCGTTCTGG ACGGTGGAAT GAACAAGATG 
AAGTCCACCG GCCTGCTGAC AGTCCCGTCC CCTCAGATAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001074106.2
CDS	1..2682
Translation	MQPLRDAELGASTFFASPLPHDVCGSNGLPLTPNSIKILGRFQILKTITHPRLCQYVDISRGKHERLVIVAEHYEKSLKDLLKQRKPVSPEKVLQVAYEVLEGLEFMNKHGMVHRALSPNNVLLDRADKVKLAKFGLYHMTDHGADVDFPIGYPSYLPPEVIAQGCFYSSEPSINEAPQPSGPKTDVWSLGVLLFELCVGRQILQNIDISERMKFIITLGCMDDIITVLAEEHGCIDTIKDLPENVQALLRKCLTFLPSKRPTPSDLLGDAVFESISSEYTPYQSPVHLFSASPRCAYLQLPDDISELCRENGENYLSERAIDEVYHLWCLAGGDLEKELTNQEIIQSKPPICTLPKFILEDGESFGQGRDRSFLLDDTTVTLSLFQLRNRLKDVAGETYFPLLEDEQCSLPQSSSSNELSATVTLPLIIRERDTEYQLTRIVLFHRLLKAYPYKKNQIWKEARVDIPPLLRGLAWAALLGVEGDIQSKYDSIDKDTPIPTDRQIEVDIPRCHQYDELLSSPQGHVKFRRVLKAWVVSHPDLVYWQGLDSLCAPFLYLNFNNEALAYACMSAFIPKYLYNFFLKDNSHVIQEYLTVFSQMIAFHDPELSNHLNEIGFIPDLYAIPWFLTMFTHVFPLHKIFHLWDTLLLGNSSFPFCIGVAILQQLRDRLLANGFNECILLFSDLPEIDIERCVRESINLFCWTPKSATYRQHAQPNKPPSESNSFGKTPSYYASEFQELTKTDLSREPLKLSELKAEVSPRISVEDLIDLCELSGPAFFKTPVKRARTGKPKILAVDIRSLEDFSRGHIYGSINIPYTSTFGPEGELLQCPASSSLAGYRLRVIVILSSVMKNAITFATHLVKVNFPRVCVLDGGMNKMKSTGLLTVPSPQI

Target ORF information:

RefSeq Version	NM_001080637.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio TBC1 domain containing kinase (tbck), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001080637.2

ORF Insert Sequence:

ATGCAGCCCC TCAGAGATGC CGAGTTGGGG GCTTCCACCT TCTTCGCTTC TCCTCTTCCT 
CACGATGTGT GCGGCAGCAA TGGGCTTCCT CTCACCCCAA ACTCCATCAA GATCCTGGGA 
CGCTTCCAGA TCCTCAAAAC CATCACCCAT CCCAGACTGT GCCAATATGT GGACATCAGC 
AGAGGAAAGC ACGAGCGCCT GGTTATTGTA GCTGAGCACT ATGAAAAAAG CTTGAAGGAC 
CTTTTGAAGC AGCGGAAACC TGTCAGTCCA GAGAAGGTGC TACAGGTTGC CTATGAAGTG 
CTGGAAGGAT TGGAGTTCAT GAATAAGCAC GGGATGGTGC ATCGCGCTTT ATCTCCTAAC 
AATGTCCTCC TAGATCGTGC GGATAAAGTG AAGCTGGCCA AATTTGGCCT GTATCATATG 
ACCGACCATG GAGCAGATGT CGACTTCCCA ATTGGGTATC CATCTTACCT GCCTCCGGAG 
GTTATTGCTC AGGGCTGTTT CTATTCCAGC GAGCCCTCCA TCAATGAGGC CCCGCAGCCT 
TCAGGGCCCA AAACTGACGT CTGGTCTCTC GGGGTGCTTC TGTTTGAACT CTGTGTGGGA 
AGGCAGATTC TGCAGAACAT AGACATCAGC GAGAGAATGA AGTTCATCAT CACCTTAGGG 
TGCATGGATG ACATCATTAC AGTATTAGCC GAGGAACACG GGTGCATAGA CACAATTAAG 
GATCTGCCTG AAAACGTGCA GGCTTTATTG AGGAAATGCC TGACGTTTCT CCCTTCAAAA 
CGACCCACTC CTTCTGATCT TCTGGGAGAC GCAGTGTTTG AGAGCATATC TTCTGAGTAT 
ACTCCCTATC AGAGTCCTGT GCATCTGTTC TCCGCCTCAC CACGATGTGC TTATCTACAG 
CTTCCTGATG ACATCAGTGA ACTCTGTAGA GAGAATGGAG AGAACTACCT GTCGGAGAGA 
GCCATAGATG AGGTTTATCA CCTGTGGTGT CTGGCAGGGG GAGACCTGGA GAAAGAGCTC 
ACCAATCAGG AGATCATCCA GTCCAAACCA CCCATCTGCA CGCTTCCCAA GTTTATTTTG 
GAGGATGGCG AGTCGTTTGG ACAGGGCCGG GACAGAAGCT TCCTCCTGGA TGACACAACT 
GTGACACTTT CTCTCTTTCA GCTCAGAAAC AGGCTGAAGG ATGTGGCGGG AGAGACATAC 
TTTCCATTAT TGGAGGATGA GCAGTGCAGC CTGCCTCAGT CCAGCAGCAG TAATGAACTG 
TCTGCCACCG TCACACTGCC CCTCATTATC CGAGAGAGAG ACACCGAGTA CCAGCTCACA 
CGCATCGTCC TCTTCCACAG ACTGCTCAAG GCATACCCTT ACAAGAAGAA CCAGATCTGG 
AAGGAGGCGA GGGTTGACAT TCCTCCTCTT CTGAGGGGTC TGGCCTGGGC GGCTTTACTC 
GGAGTTGAGG GCGACATCCA ATCAAAATAC GACTCCATCG ATAAGGACAC GCCCATCCCC 
ACTGACAGAC AGATTGAAGT GGATATTCCT CGCTGTCACC AATATGATGA GCTGCTGTCG 
TCGCCGCAGG GCCATGTCAA GTTTCGGCGG GTGCTGAAGG CTTGGGTTGT GTCTCATCCC 
GATCTGGTTT ACTGGCAAGG CTTGGATTCA CTTTGTGCAC CTTTCCTTTA TTTAAATTTC 
AACAATGAAG CTCTTGCGTA CGCATGCATG TCGGCCTTCA TCCCCAAATA TCTGTACAAT 
TTCTTCCTGA AGGACAACTC GCATGTAATA CAAGAATATC TGACCGTCTT CTCTCAGATG 
ATTGCGTTTC ACGACCCCGA GCTCAGCAAC CACCTCAACG AGATTGGATT CATACCTGAT 
CTCTACGCAA TCCCATGGTT TCTTACAATG TTCACACACG TGTTTCCCCT GCACAAGATC 
TTCCACCTGT GGGACACTCT GCTCTTGGGA AACTCCTCGT TCCCCTTCTG CATTGGTGTA 
GCCATCTTAC AGCAGCTGAG AGACCGTCTG CTGGCCAACG GCTTCAACGA GTGCATACTG 
CTCTTCTCTG ACCTCCCTGA GATCGACATT GAACGCTGTG TTCGAGAATC CATCAACCTT 
TTTTGCTGGA CTCCTAAAAG TGCCACTTAC CGCCAGCATG CCCAGCCAAA CAAACCTCCC 
AGCGAGAGCA ACAGCTTCGG CAAAACACCT TCATATTACG CATCAGAGTT TCAAGAACTG 
ACCAAAACTG ACCTGAGCCG TGAGCCACTC AAGCTGAGCG AGCTGAAAGC AGAAGTGTCT 
CCGCGTATTT CTGTGGAAGA CCTCATTGAC CTGTGTGAGC TGAGTGGACC AGCTTTCTTT 
AAAACTCCCG TCAAGAGGGC CAGGACTGGC AAACCCAAAA TTCTTGCGGT GGACATCAGG 
AGCCTTGAAG ACTTCAGTCG AGGCCACATC TACGGCAGCA TTAATATTCC ATACACCTCC 
ACGTTTGGAC CAGAGGGAGA GCTGCTCCAG TGTCCCGCTA GCAGCAGCCT AGCCGGATAC 
AGACTCCGCG TCATCGTCAT CCTCAGCAGC GTCATGAAGA ACGCTATCAC TTTTGCGACT 
CATCTGGTGA AGGTGAATTT CCCTCGTGTT TGCGTTCTGG ACGGTGGAAT GAACAAGATG 
AAGTCCACCG GCCTGCTGAC AGTCCCGTCC CCTCAGATAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tbck ( XM_005160101.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005160158.1 Danio rerio TBC1 domain containing kinase (tbck), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa06179
Clone ID Related Accession (Same CDS sequence)	NM_001080637.2 , XM_005160101.3 , XM_005160101.4
Accession Version	XM_005160101.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2682bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	TBC domain-containing protein kinase-like protein isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005160101.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCCCC TCAGAGATGC CGAGTTGGGG GCTTCCACCT TCTTCGCTTC TCCTCTTCCT 
CACGATGTGT GCGGCAGCAA TGGGCTTCCT CTCACCCCAA ACTCCATCAA GATCCTGGGA 
CGCTTCCAGA TCCTCAAAAC CATCACCCAT CCCAGACTGT GCCAATATGT GGACATCAGC 
AGAGGAAAGC ACGAGCGCCT GGTTATTGTA GCTGAGCACT ATGAAAAAAG CTTGAAGGAC 
CTTTTGAAGC AGCGGAAACC TGTCAGTCCA GAGAAGGTGC TACAGGTTGC CTATGAAGTG 
CTGGAAGGAT TGGAGTTCAT GAATAAGCAC GGGATGGTGC ATCGCGCTTT ATCTCCTAAC 
AATGTCCTCC TAGATCGTGC GGATAAAGTG AAGCTGGCCA AATTTGGCCT GTATCATATG 
ACCGACCATG GAGCAGATGT CGACTTCCCA ATTGGGTATC CATCTTACCT GCCTCCGGAG 
GTTATTGCTC AGGGCTGTTT CTATTCCAGC GAGCCCTCCA TCAATGAGGC CCCGCAGCCT 
TCAGGGCCCA AAACTGACGT CTGGTCTCTC GGGGTGCTTC TGTTTGAACT CTGTGTGGGA 
AGGCAGATTC TGCAGAACAT AGACATCAGC GAGAGAATGA AGTTCATCAT CACCTTAGGG 
TGCATGGATG ACATCATTAC AGTATTAGCC GAGGAACACG GGTGCATAGA CACAATTAAG 
GATCTGCCTG AAAACGTGCA GGCTTTATTG AGGAAATGCC TGACGTTTCT CCCTTCAAAA 
CGACCCACTC CTTCTGATCT TCTGGGAGAC GCAGTGTTTG AGAGCATATC TTCTGAGTAT 
ACTCCCTATC AGAGTCCTGT GCATCTGTTC TCCGCCTCAC CACGATGTGC TTATCTACAG 
CTTCCTGATG ACATCAGTGA ACTCTGTAGA GAGAATGGAG AGAACTACCT GTCGGAGAGA 
GCCATAGATG AGGTTTATCA CCTGTGGTGT CTGGCAGGGG GAGACCTGGA GAAAGAGCTC 
ACCAATCAGG AGATCATCCA GTCCAAACCA CCCATCTGCA CGCTTCCCAA GTTTATTTTG 
GAGGATGGCG AGTCGTTTGG ACAGGGCCGG GACAGAAGCT TCCTCCTGGA TGACACAACT 
GTGACACTTT CTCTCTTTCA GCTCAGAAAC AGGCTGAAGG ATGTGGCGGG AGAGACATAC 
TTTCCATTAT TGGAGGATGA GCAGTGCAGC CTGCCTCAGT CCAGCAGCAG TAATGAACTG 
TCTGCCACCG TCACACTGCC CCTCATTATC CGAGAGAGAG ACACCGAGTA CCAGCTCACA 
CGCATCGTCC TCTTCCACAG ACTGCTCAAG GCATACCCTT ACAAGAAGAA CCAGATCTGG 
AAGGAGGCGA GGGTTGACAT TCCTCCTCTT CTGAGGGGTC TGGCCTGGGC GGCTTTACTC 
GGAGTTGAGG GCGACATCCA ATCAAAATAC GACTCCATCG ATAAGGACAC GCCCATCCCC 
ACTGACAGAC AGATTGAAGT GGATATTCCT CGCTGTCACC AATATGATGA GCTGCTGTCG 
TCGCCGCAGG GCCATGTCAA GTTTCGGCGG GTGCTGAAGG CTTGGGTTGT GTCTCATCCC 
GATCTGGTTT ACTGGCAAGG CTTGGATTCA CTTTGTGCAC CTTTCCTTTA TTTAAATTTC 
AACAATGAAG CTCTTGCGTA CGCATGCATG TCGGCCTTCA TCCCCAAATA TCTGTACAAT 
TTCTTCCTGA AGGACAACTC GCATGTAATA CAAGAATATC TGACCGTCTT CTCTCAGATG 
ATTGCGTTTC ACGACCCCGA GCTCAGCAAC CACCTCAACG AGATTGGATT CATACCTGAT 
CTCTACGCAA TCCCATGGTT TCTTACAATG TTCACACACG TGTTTCCCCT GCACAAGATC 
TTCCACCTGT GGGACACTCT GCTCTTGGGA AACTCCTCGT TCCCCTTCTG CATTGGTGTA 
GCCATCTTAC AGCAGCTGAG AGACCGTCTG CTGGCCAACG GCTTCAACGA GTGCATACTG 
CTCTTCTCTG ACCTCCCTGA GATCGACATT GAACGCTGTG TTCGAGAATC CATCAACCTT 
TTTTGCTGGA CTCCTAAAAG TGCCACTTAC CGCCAGCATG CCCAGCCAAA CAAACCTCCC 
AGCGAGAGCA ACAGCTTCGG CAAAACACCT TCATATTACG CATCAGAGTT TCAAGAACTG 
ACCAAAACTG ACCTGAGCCG TGAGCCACTC AAGCTGAGCG AGCTGAAAGC AGAAGTGTCT 
CCGCGTATTT CTGTGGAAGA CCTCATTGAC CTGTGTGAGC TGAGTGGACC AGCTTTCTTT 
AAAACTCCCG TCAAGAGGGC CAGGACTGGC AAACCCAAAA TTCTTGCGGT GGACATCAGG 
AGCCTTGAAG ACTTCAGTCG AGGCCACATC TACGGCAGCA TTAATATTCC ATACACCTCC 
ACGTTTGGAC CAGAGGGAGA GCTGCTCCAG TGTCCCGCTA GCAGCAGCCT AGCCGGATAC 
AGACTCCGCG TCATCGTCAT CCTCAGCAGC GTCATGAAGA ACGCTATCAC TTTTGCGACT 
CATCTGGTGA AGGTGAATTT CCCTCGTGTT TGCGTTCTGG ACGGTGGAAT GAACAAGATG 
AAGTCCACCG GCCTGCTGAC AGTCCCGTCC CCTCAGATAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005160158.1
CDS	160..2841
Translation	MQPLRDAELGASTFFASPLPHDVCGSNGLPLTPNSIKILGRFQILKTITHPRLCQYVDISRGKHERLVIVAEHYEKSLKDLLKQRKPVSPEKVLQVAYEVLEGLEFMNKHGMVHRALSPNNVLLDRADKVKLAKFGLYHMTDHGADVDFPIGYPSYLPPEVIAQGCFYSSEPSINEAPQPSGPKTDVWSLGVLLFELCVGRQILQNIDISERMKFIITLGCMDDIITVLAEEHGCIDTIKDLPENVQALLRKCLTFLPSKRPTPSDLLGDAVFESISSEYTPYQSPVHLFSASPRCAYLQLPDDISELCRENGENYLSERAIDEVYHLWCLAGGDLEKELTNQEIIQSKPPICTLPKFILEDGESFGQGRDRSFLLDDTTVTLSLFQLRNRLKDVAGETYFPLLEDEQCSLPQSSSSNELSATVTLPLIIRERDTEYQLTRIVLFHRLLKAYPYKKNQIWKEARVDIPPLLRGLAWAALLGVEGDIQSKYDSIDKDTPIPTDRQIEVDIPRCHQYDELLSSPQGHVKFRRVLKAWVVSHPDLVYWQGLDSLCAPFLYLNFNNEALAYACMSAFIPKYLYNFFLKDNSHVIQEYLTVFSQMIAFHDPELSNHLNEIGFIPDLYAIPWFLTMFTHVFPLHKIFHLWDTLLLGNSSFPFCIGVAILQQLRDRLLANGFNECILLFSDLPEIDIERCVRESINLFCWTPKSATYRQHAQPNKPPSESNSFGKTPSYYASEFQELTKTDLSREPLKLSELKAEVSPRISVEDLIDLCELSGPAFFKTPVKRARTGKPKILAVDIRSLEDFSRGHIYGSINIPYTSTFGPEGELLQCPASSSLAGYRLRVIVILSSVMKNAITFATHLVKVNFPRVCVLDGGMNKMKSTGLLTVPSPQI

Target ORF information:

RefSeq Version	XM_005160101.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio TBC1 domain containing kinase (tbck), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005160101.3

ORF Insert Sequence:

ATGCAGCCCC TCAGAGATGC CGAGTTGGGG GCTTCCACCT TCTTCGCTTC TCCTCTTCCT 
CACGATGTGT GCGGCAGCAA TGGGCTTCCT CTCACCCCAA ACTCCATCAA GATCCTGGGA 
CGCTTCCAGA TCCTCAAAAC CATCACCCAT CCCAGACTGT GCCAATATGT GGACATCAGC 
AGAGGAAAGC ACGAGCGCCT GGTTATTGTA GCTGAGCACT ATGAAAAAAG CTTGAAGGAC 
CTTTTGAAGC AGCGGAAACC TGTCAGTCCA GAGAAGGTGC TACAGGTTGC CTATGAAGTG 
CTGGAAGGAT TGGAGTTCAT GAATAAGCAC GGGATGGTGC ATCGCGCTTT ATCTCCTAAC 
AATGTCCTCC TAGATCGTGC GGATAAAGTG AAGCTGGCCA AATTTGGCCT GTATCATATG 
ACCGACCATG GAGCAGATGT CGACTTCCCA ATTGGGTATC CATCTTACCT GCCTCCGGAG 
GTTATTGCTC AGGGCTGTTT CTATTCCAGC GAGCCCTCCA TCAATGAGGC CCCGCAGCCT 
TCAGGGCCCA AAACTGACGT CTGGTCTCTC GGGGTGCTTC TGTTTGAACT CTGTGTGGGA 
AGGCAGATTC TGCAGAACAT AGACATCAGC GAGAGAATGA AGTTCATCAT CACCTTAGGG 
TGCATGGATG ACATCATTAC AGTATTAGCC GAGGAACACG GGTGCATAGA CACAATTAAG 
GATCTGCCTG AAAACGTGCA GGCTTTATTG AGGAAATGCC TGACGTTTCT CCCTTCAAAA 
CGACCCACTC CTTCTGATCT TCTGGGAGAC GCAGTGTTTG AGAGCATATC TTCTGAGTAT 
ACTCCCTATC AGAGTCCTGT GCATCTGTTC TCCGCCTCAC CACGATGTGC TTATCTACAG 
CTTCCTGATG ACATCAGTGA ACTCTGTAGA GAGAATGGAG AGAACTACCT GTCGGAGAGA 
GCCATAGATG AGGTTTATCA CCTGTGGTGT CTGGCAGGGG GAGACCTGGA GAAAGAGCTC 
ACCAATCAGG AGATCATCCA GTCCAAACCA CCCATCTGCA CGCTTCCCAA GTTTATTTTG 
GAGGATGGCG AGTCGTTTGG ACAGGGCCGG GACAGAAGCT TCCTCCTGGA TGACACAACT 
GTGACACTTT CTCTCTTTCA GCTCAGAAAC AGGCTGAAGG ATGTGGCGGG AGAGACATAC 
TTTCCATTAT TGGAGGATGA GCAGTGCAGC CTGCCTCAGT CCAGCAGCAG TAATGAACTG 
TCTGCCACCG TCACACTGCC CCTCATTATC CGAGAGAGAG ACACCGAGTA CCAGCTCACA 
CGCATCGTCC TCTTCCACAG ACTGCTCAAG GCATACCCTT ACAAGAAGAA CCAGATCTGG 
AAGGAGGCGA GGGTTGACAT TCCTCCTCTT CTGAGGGGTC TGGCCTGGGC GGCTTTACTC 
GGAGTTGAGG GCGACATCCA ATCAAAATAC GACTCCATCG ATAAGGACAC GCCCATCCCC 
ACTGACAGAC AGATTGAAGT GGATATTCCT CGCTGTCACC AATATGATGA GCTGCTGTCG 
TCGCCGCAGG GCCATGTCAA GTTTCGGCGG GTGCTGAAGG CTTGGGTTGT GTCTCATCCC 
GATCTGGTTT ACTGGCAAGG CTTGGATTCA CTTTGTGCAC CTTTCCTTTA TTTAAATTTC 
AACAATGAAG CTCTTGCGTA CGCATGCATG TCGGCCTTCA TCCCCAAATA TCTGTACAAT 
TTCTTCCTGA AGGACAACTC GCATGTAATA CAAGAATATC TGACCGTCTT CTCTCAGATG 
ATTGCGTTTC ACGACCCCGA GCTCAGCAAC CACCTCAACG AGATTGGATT CATACCTGAT 
CTCTACGCAA TCCCATGGTT TCTTACAATG TTCACACACG TGTTTCCCCT GCACAAGATC 
TTCCACCTGT GGGACACTCT GCTCTTGGGA AACTCCTCGT TCCCCTTCTG CATTGGTGTA 
GCCATCTTAC AGCAGCTGAG AGACCGTCTG CTGGCCAACG GCTTCAACGA GTGCATACTG 
CTCTTCTCTG ACCTCCCTGA GATCGACATT GAACGCTGTG TTCGAGAATC CATCAACCTT 
TTTTGCTGGA CTCCTAAAAG TGCCACTTAC CGCCAGCATG CCCAGCCAAA CAAACCTCCC 
AGCGAGAGCA ACAGCTTCGG CAAAACACCT TCATATTACG CATCAGAGTT TCAAGAACTG 
ACCAAAACTG ACCTGAGCCG TGAGCCACTC AAGCTGAGCG AGCTGAAAGC AGAAGTGTCT 
CCGCGTATTT CTGTGGAAGA CCTCATTGAC CTGTGTGAGC TGAGTGGACC AGCTTTCTTT 
AAAACTCCCG TCAAGAGGGC CAGGACTGGC AAACCCAAAA TTCTTGCGGT GGACATCAGG 
AGCCTTGAAG ACTTCAGTCG AGGCCACATC TACGGCAGCA TTAATATTCC ATACACCTCC 
ACGTTTGGAC CAGAGGGAGA GCTGCTCCAG TGTCCCGCTA GCAGCAGCCT AGCCGGATAC 
AGACTCCGCG TCATCGTCAT CCTCAGCAGC GTCATGAAGA ACGCTATCAC TTTTGCGACT 
CATCTGGTGA AGGTGAATTT CCCTCGTGTT TGCGTTCTGG ACGGTGGAAT GAACAAGATG 
AAGTCCACCG GCCTGCTGAC AGTCCCGTCC CCTCAGATAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tbck ( XM_005160101.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005160158.1 Danio rerio TBC1 domain containing kinase (tbck), transcript variant X1, mRNA.

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CloneID	ODa06179
Clone ID Related Accession (Same CDS sequence)	NM_001080637.2 , XM_005160101.3 , XM_005160101.4
Accession Version	XM_005160101.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2682bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	TBC domain-containing protein kinase-like protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005160101.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCCCC TCAGAGATGC CGAGTTGGGG GCTTCCACCT TCTTCGCTTC TCCTCTTCCT 
CACGATGTGT GCGGCAGCAA TGGGCTTCCT CTCACCCCAA ACTCCATCAA GATCCTGGGA 
CGCTTCCAGA TCCTCAAAAC CATCACCCAT CCCAGACTGT GCCAATATGT GGACATCAGC 
AGAGGAAAGC ACGAGCGCCT GGTTATTGTA GCTGAGCACT ATGAAAAAAG CTTGAAGGAC 
CTTTTGAAGC AGCGGAAACC TGTCAGTCCA GAGAAGGTGC TACAGGTTGC CTATGAAGTG 
CTGGAAGGAT TGGAGTTCAT GAATAAGCAC GGGATGGTGC ATCGCGCTTT ATCTCCTAAC 
AATGTCCTCC TAGATCGTGC GGATAAAGTG AAGCTGGCCA AATTTGGCCT GTATCATATG 
ACCGACCATG GAGCAGATGT CGACTTCCCA ATTGGGTATC CATCTTACCT GCCTCCGGAG 
GTTATTGCTC AGGGCTGTTT CTATTCCAGC GAGCCCTCCA TCAATGAGGC CCCGCAGCCT 
TCAGGGCCCA AAACTGACGT CTGGTCTCTC GGGGTGCTTC TGTTTGAACT CTGTGTGGGA 
AGGCAGATTC TGCAGAACAT AGACATCAGC GAGAGAATGA AGTTCATCAT CACCTTAGGG 
TGCATGGATG ACATCATTAC AGTATTAGCC GAGGAACACG GGTGCATAGA CACAATTAAG 
GATCTGCCTG AAAACGTGCA GGCTTTATTG AGGAAATGCC TGACGTTTCT CCCTTCAAAA 
CGACCCACTC CTTCTGATCT TCTGGGAGAC GCAGTGTTTG AGAGCATATC TTCTGAGTAT 
ACTCCCTATC AGAGTCCTGT GCATCTGTTC TCCGCCTCAC CACGATGTGC TTATCTACAG 
CTTCCTGATG ACATCAGTGA ACTCTGTAGA GAGAATGGAG AGAACTACCT GTCGGAGAGA 
GCCATAGATG AGGTTTATCA CCTGTGGTGT CTGGCAGGGG GAGACCTGGA GAAAGAGCTC 
ACCAATCAGG AGATCATCCA GTCCAAACCA CCCATCTGCA CGCTTCCCAA GTTTATTTTG 
GAGGATGGCG AGTCGTTTGG ACAGGGCCGG GACAGAAGCT TCCTCCTGGA TGACACAACT 
GTGACACTTT CTCTCTTTCA GCTCAGAAAC AGGCTGAAGG ATGTGGCGGG AGAGACATAC 
TTTCCATTAT TGGAGGATGA GCAGTGCAGC CTGCCTCAGT CCAGCAGCAG TAATGAACTG 
TCTGCCACCG TCACACTGCC CCTCATTATC CGAGAGAGAG ACACCGAGTA CCAGCTCACA 
CGCATCGTCC TCTTCCACAG ACTGCTCAAG GCATACCCTT ACAAGAAGAA CCAGATCTGG 
AAGGAGGCGA GGGTTGACAT TCCTCCTCTT CTGAGGGGTC TGGCCTGGGC GGCTTTACTC 
GGAGTTGAGG GCGACATCCA ATCAAAATAC GACTCCATCG ATAAGGACAC GCCCATCCCC 
ACTGACAGAC AGATTGAAGT GGATATTCCT CGCTGTCACC AATATGATGA GCTGCTGTCG 
TCGCCGCAGG GCCATGTCAA GTTTCGGCGG GTGCTGAAGG CTTGGGTTGT GTCTCATCCC 
GATCTGGTTT ACTGGCAAGG CTTGGATTCA CTTTGTGCAC CTTTCCTTTA TTTAAATTTC 
AACAATGAAG CTCTTGCGTA CGCATGCATG TCGGCCTTCA TCCCCAAATA TCTGTACAAT 
TTCTTCCTGA AGGACAACTC GCATGTAATA CAAGAATATC TGACCGTCTT CTCTCAGATG 
ATTGCGTTTC ACGACCCCGA GCTCAGCAAC CACCTCAACG AGATTGGATT CATACCTGAT 
CTCTACGCAA TCCCATGGTT TCTTACAATG TTCACACACG TGTTTCCCCT GCACAAGATC 
TTCCACCTGT GGGACACTCT GCTCTTGGGA AACTCCTCGT TCCCCTTCTG CATTGGTGTA 
GCCATCTTAC AGCAGCTGAG AGACCGTCTG CTGGCCAACG GCTTCAACGA GTGCATACTG 
CTCTTCTCTG ACCTCCCTGA GATCGACATT GAACGCTGTG TTCGAGAATC CATCAACCTT 
TTTTGCTGGA CTCCTAAAAG TGCCACTTAC CGCCAGCATG CCCAGCCAAA CAAACCTCCC 
AGCGAGAGCA ACAGCTTCGG CAAAACACCT TCATATTACG CATCAGAGTT TCAAGAACTG 
ACCAAAACTG ACCTGAGCCG TGAGCCACTC AAGCTGAGCG AGCTGAAAGC AGAAGTGTCT 
CCGCGTATTT CTGTGGAAGA CCTCATTGAC CTGTGTGAGC TGAGTGGACC AGCTTTCTTT 
AAAACTCCCG TCAAGAGGGC CAGGACTGGC AAACCCAAAA TTCTTGCGGT GGACATCAGG 
AGCCTTGAAG ACTTCAGTCG AGGCCACATC TACGGCAGCA TTAATATTCC ATACACCTCC 
ACGTTTGGAC CAGAGGGAGA GCTGCTCCAG TGTCCCGCTA GCAGCAGCCT AGCCGGATAC 
AGACTCCGCG TCATCGTCAT CCTCAGCAGC GTCATGAAGA ACGCTATCAC TTTTGCGACT 
CATCTGGTGA AGGTGAATTT CCCTCGTGTT TGCGTTCTGG ACGGTGGAAT GAACAAGATG 
AAGTCCACCG GCCTGCTGAC AGTCCCGTCC CCTCAGATAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005160158.1
CDS	170..2851
Translation	MQPLRDAELGASTFFASPLPHDVCGSNGLPLTPNSIKILGRFQILKTITHPRLCQYVDISRGKHERLVIVAEHYEKSLKDLLKQRKPVSPEKVLQVAYEVLEGLEFMNKHGMVHRALSPNNVLLDRADKVKLAKFGLYHMTDHGADVDFPIGYPSYLPPEVIAQGCFYSSEPSINEAPQPSGPKTDVWSLGVLLFELCVGRQILQNIDISERMKFIITLGCMDDIITVLAEEHGCIDTIKDLPENVQALLRKCLTFLPSKRPTPSDLLGDAVFESISSEYTPYQSPVHLFSASPRCAYLQLPDDISELCRENGENYLSERAIDEVYHLWCLAGGDLEKELTNQEIIQSKPPICTLPKFILEDGESFGQGRDRSFLLDDTTVTLSLFQLRNRLKDVAGETYFPLLEDEQCSLPQSSSSNELSATVTLPLIIRERDTEYQLTRIVLFHRLLKAYPYKKNQIWKEARVDIPPLLRGLAWAALLGVEGDIQSKYDSIDKDTPIPTDRQIEVDIPRCHQYDELLSSPQGHVKFRRVLKAWVVSHPDLVYWQGLDSLCAPFLYLNFNNEALAYACMSAFIPKYLYNFFLKDNSHVIQEYLTVFSQMIAFHDPELSNHLNEIGFIPDLYAIPWFLTMFTHVFPLHKIFHLWDTLLLGNSSFPFCIGVAILQQLRDRLLANGFNECILLFSDLPEIDIERCVRESINLFCWTPKSATYRQHAQPNKPPSESNSFGKTPSYYASEFQELTKTDLSREPLKLSELKAEVSPRISVEDLIDLCELSGPAFFKTPVKRARTGKPKILAVDIRSLEDFSRGHIYGSINIPYTSTFGPEGELLQCPASSSLAGYRLRVIVILSSVMKNAITFATHLVKVNFPRVCVLDGGMNKMKSTGLLTVPSPQI

Target ORF information:

RefSeq Version	XM_005160101.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio TBC1 domain containing kinase (tbck), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005160101.4

ORF Insert Sequence:

ATGCAGCCCC TCAGAGATGC CGAGTTGGGG GCTTCCACCT TCTTCGCTTC TCCTCTTCCT 
CACGATGTGT GCGGCAGCAA TGGGCTTCCT CTCACCCCAA ACTCCATCAA GATCCTGGGA 
CGCTTCCAGA TCCTCAAAAC CATCACCCAT CCCAGACTGT GCCAATATGT GGACATCAGC 
AGAGGAAAGC ACGAGCGCCT GGTTATTGTA GCTGAGCACT ATGAAAAAAG CTTGAAGGAC 
CTTTTGAAGC AGCGGAAACC TGTCAGTCCA GAGAAGGTGC TACAGGTTGC CTATGAAGTG 
CTGGAAGGAT TGGAGTTCAT GAATAAGCAC GGGATGGTGC ATCGCGCTTT ATCTCCTAAC 
AATGTCCTCC TAGATCGTGC GGATAAAGTG AAGCTGGCCA AATTTGGCCT GTATCATATG 
ACCGACCATG GAGCAGATGT CGACTTCCCA ATTGGGTATC CATCTTACCT GCCTCCGGAG 
GTTATTGCTC AGGGCTGTTT CTATTCCAGC GAGCCCTCCA TCAATGAGGC CCCGCAGCCT 
TCAGGGCCCA AAACTGACGT CTGGTCTCTC GGGGTGCTTC TGTTTGAACT CTGTGTGGGA 
AGGCAGATTC TGCAGAACAT AGACATCAGC GAGAGAATGA AGTTCATCAT CACCTTAGGG 
TGCATGGATG ACATCATTAC AGTATTAGCC GAGGAACACG GGTGCATAGA CACAATTAAG 
GATCTGCCTG AAAACGTGCA GGCTTTATTG AGGAAATGCC TGACGTTTCT CCCTTCAAAA 
CGACCCACTC CTTCTGATCT TCTGGGAGAC GCAGTGTTTG AGAGCATATC TTCTGAGTAT 
ACTCCCTATC AGAGTCCTGT GCATCTGTTC TCCGCCTCAC CACGATGTGC TTATCTACAG 
CTTCCTGATG ACATCAGTGA ACTCTGTAGA GAGAATGGAG AGAACTACCT GTCGGAGAGA 
GCCATAGATG AGGTTTATCA CCTGTGGTGT CTGGCAGGGG GAGACCTGGA GAAAGAGCTC 
ACCAATCAGG AGATCATCCA GTCCAAACCA CCCATCTGCA CGCTTCCCAA GTTTATTTTG 
GAGGATGGCG AGTCGTTTGG ACAGGGCCGG GACAGAAGCT TCCTCCTGGA TGACACAACT 
GTGACACTTT CTCTCTTTCA GCTCAGAAAC AGGCTGAAGG ATGTGGCGGG AGAGACATAC 
TTTCCATTAT TGGAGGATGA GCAGTGCAGC CTGCCTCAGT CCAGCAGCAG TAATGAACTG 
TCTGCCACCG TCACACTGCC CCTCATTATC CGAGAGAGAG ACACCGAGTA CCAGCTCACA 
CGCATCGTCC TCTTCCACAG ACTGCTCAAG GCATACCCTT ACAAGAAGAA CCAGATCTGG 
AAGGAGGCGA GGGTTGACAT TCCTCCTCTT CTGAGGGGTC TGGCCTGGGC GGCTTTACTC 
GGAGTTGAGG GCGACATCCA ATCAAAATAC GACTCCATCG ATAAGGACAC GCCCATCCCC 
ACTGACAGAC AGATTGAAGT GGATATTCCT CGCTGTCACC AATATGATGA GCTGCTGTCG 
TCGCCGCAGG GCCATGTCAA GTTTCGGCGG GTGCTGAAGG CTTGGGTTGT GTCTCATCCC 
GATCTGGTTT ACTGGCAAGG CTTGGATTCA CTTTGTGCAC CTTTCCTTTA TTTAAATTTC 
AACAATGAAG CTCTTGCGTA CGCATGCATG TCGGCCTTCA TCCCCAAATA TCTGTACAAT 
TTCTTCCTGA AGGACAACTC GCATGTAATA CAAGAATATC TGACCGTCTT CTCTCAGATG 
ATTGCGTTTC ACGACCCCGA GCTCAGCAAC CACCTCAACG AGATTGGATT CATACCTGAT 
CTCTACGCAA TCCCATGGTT TCTTACAATG TTCACACACG TGTTTCCCCT GCACAAGATC 
TTCCACCTGT GGGACACTCT GCTCTTGGGA AACTCCTCGT TCCCCTTCTG CATTGGTGTA 
GCCATCTTAC AGCAGCTGAG AGACCGTCTG CTGGCCAACG GCTTCAACGA GTGCATACTG 
CTCTTCTCTG ACCTCCCTGA GATCGACATT GAACGCTGTG TTCGAGAATC CATCAACCTT 
TTTTGCTGGA CTCCTAAAAG TGCCACTTAC CGCCAGCATG CCCAGCCAAA CAAACCTCCC 
AGCGAGAGCA ACAGCTTCGG CAAAACACCT TCATATTACG CATCAGAGTT TCAAGAACTG 
ACCAAAACTG ACCTGAGCCG TGAGCCACTC AAGCTGAGCG AGCTGAAAGC AGAAGTGTCT 
CCGCGTATTT CTGTGGAAGA CCTCATTGAC CTGTGTGAGC TGAGTGGACC AGCTTTCTTT 
AAAACTCCCG TCAAGAGGGC CAGGACTGGC AAACCCAAAA TTCTTGCGGT GGACATCAGG 
AGCCTTGAAG ACTTCAGTCG AGGCCACATC TACGGCAGCA TTAATATTCC ATACACCTCC 
ACGTTTGGAC CAGAGGGAGA GCTGCTCCAG TGTCCCGCTA GCAGCAGCCT AGCCGGATAC 
AGACTCCGCG TCATCGTCAT CCTCAGCAGC GTCATGAAGA ACGCTATCAC TTTTGCGACT 
CATCTGGTGA AGGTGAATTT CCCTCGTGTT TGCGTTCTGG ACGGTGGAAT GAACAAGATG 
AAGTCCACCG GCCTGCTGAC AGTCCCGTCC CCTCAGATAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium.
Briefings in bioinformatics12(5)449-62(2011 Sep)
Gaudet P,Livstone MS,Lewis SE,Thomas PD

Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.
Proceedings of the National Academy of Sciences of the United States of America99(26)16899-903(2002 Dec)
Strausberg RL,Feingold EA,Grouse LH,Derge JG,Klausner RD,Collins FS,Wagner L,Shenmen CM,Schuler GD,Altschul SF,Zeeberg B,Buetow KH,Schaefer CF,Bhat NK,Hopkins RF,Jordan H,Moore T,Max SI,Wang J,Hsieh F,Diatchenko L,Marusina K,Farmer AA,Rubin GM,Hong L,Stapleton M,Soares MB,Bonaldo MF,Casavant TL,Scheetz TE,Brownstein MJ,Usdin TB,Toshiyuki S,Carninci P,Prange C,Raha SS,Loquellano NA,Peters GJ,Abramson RD,Mullahy SJ,Bosak SA,McEwan PJ,McKernan KJ,Malek JA,Gunaratne PH,Richards S,Worley KC,Hale S,Garcia AM,Gay LJ,Hulyk SW,Villalon DK,Muzny DM,Sodergren EJ,Lu X,Gibbs RA,Fahey J,Helton E,Ketteman M,Madan A,Rodrigues S,Sanchez A,Whiting M,Madan A,Young AC,Shevchenko Y,Bouffard GG,Blakesley RW,Touchman JW,Green ED,Dickson MC,Rodriguez AC,Grimwood J,Schmutz J,Myers RM,Butterfield YS,Krzywinski MI,Skalska U,Smailus DE,Schnerch A,Schein JE,Jones SJ,Marra MA,

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tbck cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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