pde8a cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	pde8a
Entrez Gene ID	791145
Full Name	phosphodiesterase 8A
Synonyms	zgc:158458
General protein information	Preferred Names phosphodiesterase 8A Names high affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8A
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001080627.1	NP_001074096.1	high affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8A

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	PDE8A	XP_001163046.1
Homo sapiens (human)	PDE8A	NP_002596.1
Mus musculus (house mouse)	Pde8a	NP_032829.1
Caenorhabditis elegans (roundworm)	pde-6	NP_490787.1
	PDE8A	XP_001086066.1
Rattus norvegicus (Norway rat)	Pde8a	NP_942062.1
Danio rerio (zebrafish)	pde8a	NP_001074096.1
Canis lupus familiaris (dog)	PDE8A	XP_005618438.1
Xenopus tropicalis (tropical clawed frog)	pde8a	XP_002939668.2
Bos taurus (cattle)	PDE8A	NP_001178384.1
Gallus gallus (chicken)	PDE8A	XP_413834.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following pde8a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the pde8a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa33411	NM_001080627.1 Latest version!	Danio rerio phosphodiesterase 8A (pde8a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

pde8a ( NM_001080627.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001074096.1 Danio rerio phosphodiesterase 8A (pde8a), mRNA.

Price & Availability↑

CloneID	ODa33411
Clone ID Related Accession (Same CDS sequence)	NM_001080627.1
Accession Version	NM_001080627.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2454bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	high affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8A
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC129238.1. ##Evidence-Data-START## Transcript exon combination :: BC129238.1 [ECO:0000332] ##Evidence-Data-END##

ATGGGCTGCG CCTCCAGCAT CCACATCTCC GACCGGGTGG TGTATCACAG CGGGAAAGAG 
TCCGAGGAGC CGCCGCACAC ACACTCACAC ACACACACCT GCACACCGGA TACCGGAGAC 
CGCAGCAGCG CCGGCGGACA GGGCTCGCTA ACGGAGCTGC AGTTTGGACC AATGAAGCTG 
AATGAAGATC AGCTGCAGGT GCTGCTGGTG TTTGCGCGTG ATGACGCTCA GAGCAGCAGC 
TTCATCAGTG CGTGTGAGCG CTGTGGTTTC GGCTGCAGTG TGTGTCGAAC ACCAGAGGCG 
GCGCTGGAGT GTGTCCTGCA GAAGAGCCCC GACCTGATCA TTATTGACCA CCGGCACCCC 
CGAGGGCTGG ACGGAGAGGC GCTCTGCCGC TCAATCCGGG CCGTCAGCTG CTCTCACAAC 
ACTGTGATCG TGGCTGTAGT CAAAAGGCCG GATCGTGAAG AAGCCACTGT GATGCCGCTG 
ATATCAGCAG GGTTCAACAG GAGATTCGTG GAGAACTCCA GCATGATGTC CTGCTATAAT 
GAGCTCCTGC AGCTTCACCA TGGAGAGATC CGAGCACAGA TCAAACTCAG AGCCTGTAAT 
GCTTTGTTTA CTGCGCTGGA ACAGAGTCAG GAAGCTATTG AGATCAGCAG TGAAGATCAC 
ATCATACAGT ACGTCAATCC AGCGTACGAG TGCATGATGG GATACCAGTG CGGGGAGCTG 
CTGAAGGAGA ACACTGAAGT GCCTAAGAGT GAGAGAAACA GACCAGACCT GCTGGACAAC 
ATCAGCTCCT GCATGCTGCG GGGGAAGGAG TGGCAGGGGG TTTATTATGC CAAAAAGAAG 
AACGGGGACA GTGTGCAGCA GAACGTGAGG ATGACACCTG TCATTGGACA GGGAGGTAAA 
GTCAGACACT GTGTGTCTGT TAACAGGCCT TTAAATGACC ATAATAAGAC AGAGAAATCC 
TGCGAGCGTG TCCAGGCTGA ATCACAGACA GATATCCCGC AATCCAGCAA ACACAAGGAC 
CGCAGGAAAG GATCGCTGGA CGTTCGCTCG ACAACCTCCA GAGGCAGTGA TGGAAGTTCT 
CAGAGGAGAC ACTCGTCTAT GGCCAGAATA CACTCCATGA CCATAGAGGC GCCCATCACT 
AAGGTGATCA ACATCATCAA TGCGGCGCAG GAGAGCAGTC CGCTGCCGGT GGCTGAGGCT 
CTGGATCGGG TGCTGGAGAT CCTGCGCAGC TCTGAGCTCT ACTCGCCTCA GCTCGCCACC 
AAGGAGGAGG ACCCGCATAC TAATGACCTG GTGGGCGGGC TGATGACGGA CGGCCTGCGC 
AGGTTATCTG GGAACGAGTA CATCTTCACC ACTAAACAGC TGCATCACAT GACCAGTCAC 
CAGACCACGC CCCTGTCCCT CAACGACATC CCACAATGCA TCGCTCAGAC CATGGAGAAC 
GAGGACTCAT GGGACTTCGA CATATTCAAC CTGGAATCAG CCACCTTGAA GAGGCCGCTG 
GCGTTCCTGG GCCTGAAGCT GTTCTCCCGG TTCGGTGTGT GTGAGTTTCT GGGCTGTGCG 
GAGGCGACGC TGCGCTCATG GCTGCAGAAC ATCGAGGCTA GTTACCATGG CAGCAACCCA 
TACCACAACT CCACACACGC CGCAGACGTG CTGCACGCCA CCGCCTACTT CCTGTGCAAG 
GAGCGCGTCA AGCAAAGTCT GGACCCCATA GACGAGGTCG CGGCTCTGAT CGCTGCTGCT 
GTTCATGACG TGGATCATCC CGGACGCACA AACTCCTTCC TGTGCAACGC AGGAAGTGAG 
CTGGCGGTGC TGTACAACGA CACGGCGGTG CTGGAGAGTC ACCACGCCGC GCTCGCCTTC 
CAGATCACCA CACGAGACGA CAAGAGCAAC ATCTTCAGGA ACATGGAGAG GAATGAGTAC 
CGCACGCTCC GTCAGGCCAT CATTGATATG GTTCTGGCCA CTGAGATGAC CAAACACTTC 
GAGCACGTCA ACAAGTTCGT CAACAGCATC AACAAACCAC TGGCAGCGCT GGAGGAGAAC 
GGGGGCAGTG ATGATGAGGA CTCGGTGAAG AGCGTCCTGA CGTCTCCAGA GAATCGTATC 
CTGGTGAAGC GCATGTTGAT CAAGTGTGCC GACATCTCCA ACCCCTGCAG ACCCCTGGAG 
CTGTGCATCG AGTGGGCCGG ACGCATCTCT GAGGAGTACT TCGCTCAGAC GGACGAGGAG 
AAGCAGCGGG GTCTGCCGGT GGTGATGCCG GTGTTTGATC GTCACACCTG CAGCATTCCC 
AAATCCCAGA TCTCCTTCAT CGACTACTTC ATCACCGACA TGTTCGACGC CTGGGACGCG 
TTCGCCGATC TGCCCAATCT GATGCAGCAT CTGGACAATA ACTTCAAGTA CTGGAAGTCT 
CTGGACGAGC AGAAGCTGCG GAGTCTGAGA GCGCCGCCGG AGGAACACGC GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001074096.1
CDS	476..2929
Translation	MGCASSIHISDRVVYHSGKESEEPPHTHSHTHTCTPDTGDRSSAGGQGSLTELQFGPMKLNEDQLQVLLVFARDDAQSSSFISACERCGFGCSVCRTPEAALECVLQKSPDLIIIDHRHPRGLDGEALCRSIRAVSCSHNTVIVAVVKRPDREEATVMPLISAGFNRRFVENSSMMSCYNELLQLHHGEIRAQIKLRACNALFTALEQSQEAIEISSEDHIIQYVNPAYECMMGYQCGELLKENTEVPKSERNRPDLLDNISSCMLRGKEWQGVYYAKKKNGDSVQQNVRMTPVIGQGGKVRHCVSVNRPLNDHNKTEKSCERVQAESQTDIPQSSKHKDRRKGSLDVRSTTSRGSDGSSQRRHSSMARIHSMTIEAPITKVINIINAAQESSPLPVAEALDRVLEILRSSELYSPQLATKEEDPHTNDLVGGLMTDGLRRLSGNEYIFTTKQLHHMTSHQTTPLSLNDIPQCIAQTMENEDSWDFDIFNLESATLKRPLAFLGLKLFSRFGVCEFLGCAEATLRSWLQNIEASYHGSNPYHNSTHAADVLHATAYFLCKERVKQSLDPIDEVAALIAAAVHDVDHPGRTNSFLCNAGSELAVLYNDTAVLESHHAALAFQITTRDDKSNIFRNMERNEYRTLRQAIIDMVLATEMTKHFEHVNKFVNSINKPLAALEENGGSDDEDSVKSVLTSPENRILVKRMLIKCADISNPCRPLELCIEWAGRISEEYFAQTDEEKQRGLPVVMPVFDRHTCSIPKSQISFIDYFITDMFDAWDAFADLPNLMQHLDNNFKYWKSLDEQKLRSLRAPPEEHA

Target ORF information:

RefSeq Version	NM_001080627.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio phosphodiesterase 8A (pde8a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001080627.1

ORF Insert Sequence:

ATGGGCTGCG CCTCCAGCAT CCACATCTCC GACCGGGTGG TGTATCACAG CGGGAAAGAG 
TCCGAGGAGC CGCCGCACAC ACACTCACAC ACACACACCT GCACACCGGA TACCGGAGAC 
CGCAGCAGCG CCGGCGGACA GGGCTCGCTA ACGGAGCTGC AGTTTGGACC AATGAAGCTG 
AATGAAGATC AGCTGCAGGT GCTGCTGGTG TTTGCGCGTG ATGACGCTCA GAGCAGCAGC 
TTCATCAGTG CGTGTGAGCG CTGTGGTTTC GGCTGCAGTG TGTGTCGAAC ACCAGAGGCG 
GCGCTGGAGT GTGTCCTGCA GAAGAGCCCC GACCTGATCA TTATTGACCA CCGGCACCCC 
CGAGGGCTGG ACGGAGAGGC GCTCTGCCGC TCAATCCGGG CCGTCAGCTG CTCTCACAAC 
ACTGTGATCG TGGCTGTAGT CAAAAGGCCG GATCGTGAAG AAGCCACTGT GATGCCGCTG 
ATATCAGCAG GGTTCAACAG GAGATTCGTG GAGAACTCCA GCATGATGTC CTGCTATAAT 
GAGCTCCTGC AGCTTCACCA TGGAGAGATC CGAGCACAGA TCAAACTCAG AGCCTGTAAT 
GCTTTGTTTA CTGCGCTGGA ACAGAGTCAG GAAGCTATTG AGATCAGCAG TGAAGATCAC 
ATCATACAGT ACGTCAATCC AGCGTACGAG TGCATGATGG GATACCAGTG CGGGGAGCTG 
CTGAAGGAGA ACACTGAAGT GCCTAAGAGT GAGAGAAACA GACCAGACCT GCTGGACAAC 
ATCAGCTCCT GCATGCTGCG GGGGAAGGAG TGGCAGGGGG TTTATTATGC CAAAAAGAAG 
AACGGGGACA GTGTGCAGCA GAACGTGAGG ATGACACCTG TCATTGGACA GGGAGGTAAA 
GTCAGACACT GTGTGTCTGT TAACAGGCCT TTAAATGACC ATAATAAGAC AGAGAAATCC 
TGCGAGCGTG TCCAGGCTGA ATCACAGACA GATATCCCGC AATCCAGCAA ACACAAGGAC 
CGCAGGAAAG GATCGCTGGA CGTTCGCTCG ACAACCTCCA GAGGCAGTGA TGGAAGTTCT 
CAGAGGAGAC ACTCGTCTAT GGCCAGAATA CACTCCATGA CCATAGAGGC GCCCATCACT 
AAGGTGATCA ACATCATCAA TGCGGCGCAG GAGAGCAGTC CGCTGCCGGT GGCTGAGGCT 
CTGGATCGGG TGCTGGAGAT CCTGCGCAGC TCTGAGCTCT ACTCGCCTCA GCTCGCCACC 
AAGGAGGAGG ACCCGCATAC TAATGACCTG GTGGGCGGGC TGATGACGGA CGGCCTGCGC 
AGGTTATCTG GGAACGAGTA CATCTTCACC ACTAAACAGC TGCATCACAT GACCAGTCAC 
CAGACCACGC CCCTGTCCCT CAACGACATC CCACAATGCA TCGCTCAGAC CATGGAGAAC 
GAGGACTCAT GGGACTTCGA CATATTCAAC CTGGAATCAG CCACCTTGAA GAGGCCGCTG 
GCGTTCCTGG GCCTGAAGCT GTTCTCCCGG TTCGGTGTGT GTGAGTTTCT GGGCTGTGCG 
GAGGCGACGC TGCGCTCATG GCTGCAGAAC ATCGAGGCTA GTTACCATGG CAGCAACCCA 
TACCACAACT CCACACACGC CGCAGACGTG CTGCACGCCA CCGCCTACTT CCTGTGCAAG 
GAGCGCGTCA AGCAAAGTCT GGACCCCATA GACGAGGTCG CGGCTCTGAT CGCTGCTGCT 
GTTCATGACG TGGATCATCC CGGACGCACA AACTCCTTCC TGTGCAACGC AGGAAGTGAG 
CTGGCGGTGC TGTACAACGA CACGGCGGTG CTGGAGAGTC ACCACGCCGC GCTCGCCTTC 
CAGATCACCA CACGAGACGA CAAGAGCAAC ATCTTCAGGA ACATGGAGAG GAATGAGTAC 
CGCACGCTCC GTCAGGCCAT CATTGATATG GTTCTGGCCA CTGAGATGAC CAAACACTTC 
GAGCACGTCA ACAAGTTCGT CAACAGCATC AACAAACCAC TGGCAGCGCT GGAGGAGAAC 
GGGGGCAGTG ATGATGAGGA CTCGGTGAAG AGCGTCCTGA CGTCTCCAGA GAATCGTATC 
CTGGTGAAGC GCATGTTGAT CAAGTGTGCC GACATCTCCA ACCCCTGCAG ACCCCTGGAG 
CTGTGCATCG AGTGGGCCGG ACGCATCTCT GAGGAGTACT TCGCTCAGAC GGACGAGGAG 
AAGCAGCGGG GTCTGCCGGT GGTGATGCCG GTGTTTGATC GTCACACCTG CAGCATTCCC 
AAATCCCAGA TCTCCTTCAT CGACTACTTC ATCACCGACA TGTTCGACGC CTGGGACGCG 
TTCGCCGATC TGCCCAATCT GATGCAGCAT CTGGACAATA ACTTCAAGTA CTGGAAGTCT 
CTGGACGAGC AGAAGCTGCG GAGTCTGAGA GCGCCGCCGG AGGAACACGC GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

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pde8a cDNA ORF clone, Danio rerio(zebrafish)

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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