NOD2 cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	NOD2
Entrez Gene ID	777696
Full Name	nucleotide-binding oligomerization domain containing 2
Synonyms	card15
General protein information	Preferred Names nucleotide-binding oligomerization domain containing 2 Names nucleotide-binding oligomerization domain-containing protein 2 NLR-A2 NOD2a protein NOD2b protein caspase recruitment domain family, member 15
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001328044.1	NP_001314973.1	nucleotide-binding oligomerization domain-containing protein 2
XM_009303353.2	XP_009301628.1	nucleotide-binding oligomerization domain-containing protein 2 isoform X1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	NOD2	XP_005617627.1
Homo sapiens (human)	NOD2	NP_071445.1
Bos taurus (cattle)	NOD2	NP_001002889.1
Pan troglodytes (chimpanzee)	NOD2	NP_001098710.1
	NOD2	XP_001084287.1
Mus musculus (house mouse)	Nod2	NP_665856.2
Rattus norvegicus (Norway rat)	Nod2	NP_001099642.1
Danio rerio (zebrafish)	nod2	XP_697924.3

Gene Ontology
Bibliography

Related articles in PubMed

Deletion of mstna and mstnb impairs the immune system and affects growth performance in zebrafish.
Wang C, Chen YL, Bian WP, Xie SL, Qi GL, Liu L, Strauss PR, Zou JX, Pei DS
Fish & shellfish immunology72572-580(2018 Jan)

Srivastava N, Shelly A, Kumar M, Pant A, Das B, Majumdar T, Mazumder S
Cell death discovery317067(2017)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The expression of NOD2 and RIPK2 in ZF4 cells was constitutive and induced by infection with Edwardsiella tarda and spring viremia of carp virus (SVCV). NOD2 and RIPK2 can activate NF-kappaB and IFN promoters, inducing significantly antiviral defense against SVCV infection. RIPK2 also has a role in inhibiting the bacterial replication.
Title: NOD2 in zebrafish functions in antibacterial and also antiviral responses via NF-?B, and also MDA5, RIG-I and MAVS.

our study has provided novel insights into the RIP2-mediated CARD-CARD interaction in zebrafish NOD1 and NOD2, which will be helpful to understand the molecular basis of the NOD1/2 signaling mechanism.
Title: Structural and functional insights into CARDs of zebrafish (Danio rerio) NOD1 and NOD2, and their interaction with adaptor protein RIP2.

Depletion of Nod1 or Nod2 decreased expression of dual oxidase in the intestinal epithelium and impaired the ability of larvae to reduce intracellular bacterial burden.
Title: The inflammatory bowel disease (IBD) susceptibility genes NOD1 and NOD2 have conserved anti-bacterial roles in zebrafish.

The following NOD2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NOD2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa13262	NM_001328044.1 Latest version!	Danio rerio nucleotide-binding oligomerization domain containing 2 (nod2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
ODa46585	XM_009303353.2 Latest version!	Danio rerio nucleotide-binding oligomerization domain containing 2 (nod2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NOD2 ( NM_001328044.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> NP_001314973.1 Danio rerio nucleotide-binding oligomerization domain containing 2 (nod2), mRNA.

Price & Availability↑

CloneID	ODa13262
Clone ID Related Accession (Same CDS sequence)	NM_001328044.1
Accession Version	NM_001328044.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2943bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-25
Organism	Danio rerio(zebrafish)
Product	nucleotide-binding oligomerization domain-containing protein 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CU929130.5. On Jun 3, 2016 this sequence version replaced XM_692832.6. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: GFIL01028395.1, HM133904.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGAACGCTC AACAGTTGAT CCTCAAACAG CGAGCAGAGC TTCTGGCAGT TCTATGTGGT 
GGAGGTAGCG ATGAACCTCT GGAGAGTGTT TTGGACCTGC TACTTGCTCA GGAAGTTCTT 
GTCTGGGAGG ATTACCTTAG GGTACGAGTG GCAGAGAAAC CCTTGTGTGC TAACATCAGG 
CAACTTTTAG ATTTGGTGTA TGACAAAGGA GAAGATGCAT GCAGTTATTT TTTGGCTGCG 
ATTGAGCAAG AATTAGCTGA GGAGCAAAAG GCTGGACTAT GTTTTGGTAA TGGATGCGTT 
ATGGTGGGCA AGGACAGACC AGCCACTGCA ACTTCAACAT TACTGGCTGA CAGGCCAATG 
TTAGTGAGAC GGCTCAGAGA TAATATTGAT GGAGCACTAA ATATTTTACT GACGACTGGT 
TGTTTCTCCA TCAAAGATTG TGATAGTGTG CAGCTACCTG TATACACACC CTCACAACAG 
GTTCGAAGAC TGTTAGACCA AGTAAAGTTT AAAGGGGAAA CTGCTGCAAA AACCTTACTG 
GAATATCTAG AGCAACCTGA GCCAACCTCA CCAATCTCAG CAGAGAAAGA AAATACACCT 
TCCGCTGATT GTCTGGTCTA CCAGAAGAAG CTGCGAAGCT CTGTTGCATC GCAGTCCCTC 
TTCCTTAGCA CCTATGGCGG GACAGGGCGT TTCTCATTGG ATGACATTTA CACTGATGGA 
CATCTTGAAG TGATGAATAG CTCTGGTGAA ACTACAACAT TAGGGCTGGA GGATGTAGTG 
GGTCCCATGG GGACTCTTAA TGAAGACGCT GACACCGTTC TTGTGTCTGG GGAGGCAGGA 
AGTGGCAAAA GCACATTAGT TCAGCGACTG CACTTGCTCT GGGCAAGAGA AGCTTTGCTG 
CTAAACACTT TTCTGCTGTT CCCCTTCAGT TGCCGCAAGC TAAATGCAGA GCACAGAGAG 
CTTTCTTTGA AAGAGCTCCT CTTCCTGCAC TGCTGCTGGC CTGATCGAAA CCAGGATGAG 
GTCTTCCAAT TCATCCTAGA CCACCCTCAT TTGGTGCTCT TTACCTTTGA CGGACTCGAC 
GAGTTTAAAC TGGGCTTCAC AGATGAAGAA CGTCACTGTT GCCCTACAAA GCAAGTGCCT 
ATACCTGTAT TGCTTTTCAA TCTGTTGCAG GGTACCCTAA TGAAGGGCGT TATGAAGGTG 
GTCACCAGCA GGCCACATGC TGTGGGCCCA TCACTGAAGC GCTATCTCCG CAAAGAAGTG 
CTCCTCAAGG GGTTTTCTCC TGGAGGTATT GACTGCTTTG TTAAGAAACA TTATAGTGAC 
CCTGCAATGG CTACAAGAGT CATAGAGTCT GTCCAGGGTA ACACTGCATT ACTAGGTCTT 
TGCCACATTC CTGTCTTCTG CTGGATTGTG ATCAAGTGCT ACCAGGAACT CCTGGCTGGT 
CAAGATGGTA TTCCTCAGAC CATCACCGAT GTCTACCTCC TCGTCTTACA GCATTTCTTT 
CAGCGAAAGT CATCTCAGCC CCAAAGTGGT CTGGGAAAGG CCTGGTTGGC TGAACATTTG 
GACACAGTGT TAAAGCTCGG AGAGTTGGCA CTAGAAGGTC TGCAAACCTC CTGCTATGTG 
TTCTCAGGTT ATGAGCTACA GAGAAACAGG ATCACAGAGC AAGATGTCGG CATAGGCTTC 
CTCATTTACT GCAGTGACAT ATCTGTGAAT GATTGTAAAC GTTATGAATT TCTGCACATT 
ACCTTACAGT GTTTTTTTGC TGCTCTGTAT GTTATTCTGA ATCGCAACAA TGATCGTTCT 
GCTATCTCCA GACTCTTTCA GCCTCGCAAC AGGCAGGTGT CAGGCTTGTC TCAAAGCTGT 
CTGGGACAAT GTATGGATCA TTCAGTAGAG GAGTCCCATG AGGCAGAGAC AGCAAACCTT 
CAGATTACAG CTCAGTTTGT GTCAGGACTT TTATCCCAGC GGCACAATAA TCTCCTCCTG 
GAGTGCTGTC CTGCTGCTGT GCGTGAACGC AATGTTAAGC AGGTAGTCAA GTCATTGTCC 
AAAAGGATGC AGAGACATTT CAAGTCCATT CCTCGTCCTG TAGAGGGTGA AAAGAAGAGC 
ATGCATGCCA TGCCAAGTTT TGTTTGGCTG ATCAAGTGCA TCTATGAATT GCAAGACAAC 
AGTATTGCCC AAGATGCCAT GGCTAAGTTG GATGTTGAGC ACCTGAAGCT CACCTACTGT 
AACATTGGGC CTGTTGAATG CACTGCGCTG GCATACGTGC TCAAGAACTT GCGCAAACCA 
GTTGGACTAC AGTTGGACAA CAATTCAGTG GGAGATGTGG GTGTGGAGCA GCTTCTGCCG 
TGTCTGCCTA TGTGCCACTC CCTTTACTTA AGGAACAACA ACATATCAGA TGAAGGAATA 
CGAAAACTTC TGGAAAAGGG AATAGAGTGT GAAAACTTCC AGAAAATAGC GCTTTTCAAC 
AACAAGCTCA CTGACACTTG CACCCAATAC TTTTCTTGCC TGCTAAAATC AAAGCAGAAC 
TTCCTGGCTT TGAGGCTTGG AAATAATAAC ATCACATCAG TTGGAGCAGA GCAGCTGGCA 
GAAGGCCTAA GCTACAACCA ATCTTTACAG TTTCTGGGAT TATGGGGTAA TAAAGTTGGG 
GACAGGGGAG CTGAGGTATT GGCGGATGCG CTGACGAATA GCAAAACACT AATATGGTTA 
AGTCTGGTGG ATAATGGTGT GGGCAGTGCC GGTGCATGTG CTCTGGCTAA ATTTATCCGA 
CAGAACAAAA GTCTGGAAGA ATTGTGGTTA AACAAAAACT CCATCTGCAA GGAGGGGGTT 
GATTGTCTGA TAGAGGCACT GAAAATGAAC ACTAGTGTTA AGAAAGTCTG GCTGAGAGGA 
AATAATCTGA GCCCACAGGA AGAAGAGCAG CTGAGCCAGC AAGAATGCAG ATTGACCTTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001314973.1
CDS	1028..3970
Translation	MNAQQLILKQRAELLAVLCGGGSDEPLESVLDLLLAQEVLVWEDYLRVRVAEKPLCANIRQLLDLVYDKGEDACSYFLAAIEQELAEEQKAGLCFGNGCVMVGKDRPATATSTLLADRPMLVRRLRDNIDGALNILLTTGCFSIKDCDSVQLPVYTPSQQVRRLLDQVKFKGETAAKTLLEYLEQPEPTSPISAEKENTPSADCLVYQKKLRSSVASQSLFLSTYGGTGRFSLDDIYTDGHLEVMNSSGETTTLGLEDVVGPMGTLNEDADTVLVSGEAGSGKSTLVQRLHLLWAREALLLNTFLLFPFSCRKLNAEHRELSLKELLFLHCCWPDRNQDEVFQFILDHPHLVLFTFDGLDEFKLGFTDEERHCCPTKQVPIPVLLFNLLQGTLMKGVMKVVTSRPHAVGPSLKRYLRKEVLLKGFSPGGIDCFVKKHYSDPAMATRVIESVQGNTALLGLCHIPVFCWIVIKCYQELLAGQDGIPQTITDVYLLVLQHFFQRKSSQPQSGLGKAWLAEHLDTVLKLGELALEGLQTSCYVFSGYELQRNRITEQDVGIGFLIYCSDISVNDCKRYEFLHITLQCFFAALYVILNRNNDRSAISRLFQPRNRQVSGLSQSCLGQCMDHSVEESHEAETANLQITAQFVSGLLSQRHNNLLLECCPAAVRERNVKQVVKSLSKRMQRHFKSIPRPVEGEKKSMHAMPSFVWLIKCIYELQDNSIAQDAMAKLDVEHLKLTYCNIGPVECTALAYVLKNLRKPVGLQLDNNSVGDVGVEQLLPCLPMCHSLYLRNNNISDEGIRKLLEKGIECENFQKIALFNNKLTDTCTQYFSCLLKSKQNFLALRLGNNNITSVGAEQLAEGLSYNQSLQFLGLWGNKVGDRGAEVLADALTNSKTLIWLSLVDNGVGSAGACALAKFIRQNKSLEELWLNKNSICKEGVDCLIEALKMNTSVKKVWLRGNNLSPQEEEQLSQQECRLTF

Target ORF information:

RefSeq Version	NM_001328044.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio nucleotide-binding oligomerization domain containing 2 (nod2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001328044.1

ORF Insert Sequence:

ATGAACGCTC AACAGTTGAT CCTCAAACAG CGAGCAGAGC TTCTGGCAGT TCTATGTGGT 
GGAGGTAGCG ATGAACCTCT GGAGAGTGTT TTGGACCTGC TACTTGCTCA GGAAGTTCTT 
GTCTGGGAGG ATTACCTTAG GGTACGAGTG GCAGAGAAAC CCTTGTGTGC TAACATCAGG 
CAACTTTTAG ATTTGGTGTA TGACAAAGGA GAAGATGCAT GCAGTTATTT TTTGGCTGCG 
ATTGAGCAAG AATTAGCTGA GGAGCAAAAG GCTGGACTAT GTTTTGGTAA TGGATGCGTT 
ATGGTGGGCA AGGACAGACC AGCCACTGCA ACTTCAACAT TACTGGCTGA CAGGCCAATG 
TTAGTGAGAC GGCTCAGAGA TAATATTGAT GGAGCACTAA ATATTTTACT GACGACTGGT 
TGTTTCTCCA TCAAAGATTG TGATAGTGTG CAGCTACCTG TATACACACC CTCACAACAG 
GTTCGAAGAC TGTTAGACCA AGTAAAGTTT AAAGGGGAAA CTGCTGCAAA AACCTTACTG 
GAATATCTAG AGCAACCTGA GCCAACCTCA CCAATCTCAG CAGAGAAAGA AAATACACCT 
TCCGCTGATT GTCTGGTCTA CCAGAAGAAG CTGCGAAGCT CTGTTGCATC GCAGTCCCTC 
TTCCTTAGCA CCTATGGCGG GACAGGGCGT TTCTCATTGG ATGACATTTA CACTGATGGA 
CATCTTGAAG TGATGAATAG CTCTGGTGAA ACTACAACAT TAGGGCTGGA GGATGTAGTG 
GGTCCCATGG GGACTCTTAA TGAAGACGCT GACACCGTTC TTGTGTCTGG GGAGGCAGGA 
AGTGGCAAAA GCACATTAGT TCAGCGACTG CACTTGCTCT GGGCAAGAGA AGCTTTGCTG 
CTAAACACTT TTCTGCTGTT CCCCTTCAGT TGCCGCAAGC TAAATGCAGA GCACAGAGAG 
CTTTCTTTGA AAGAGCTCCT CTTCCTGCAC TGCTGCTGGC CTGATCGAAA CCAGGATGAG 
GTCTTCCAAT TCATCCTAGA CCACCCTCAT TTGGTGCTCT TTACCTTTGA CGGACTCGAC 
GAGTTTAAAC TGGGCTTCAC AGATGAAGAA CGTCACTGTT GCCCTACAAA GCAAGTGCCT 
ATACCTGTAT TGCTTTTCAA TCTGTTGCAG GGTACCCTAA TGAAGGGCGT TATGAAGGTG 
GTCACCAGCA GGCCACATGC TGTGGGCCCA TCACTGAAGC GCTATCTCCG CAAAGAAGTG 
CTCCTCAAGG GGTTTTCTCC TGGAGGTATT GACTGCTTTG TTAAGAAACA TTATAGTGAC 
CCTGCAATGG CTACAAGAGT CATAGAGTCT GTCCAGGGTA ACACTGCATT ACTAGGTCTT 
TGCCACATTC CTGTCTTCTG CTGGATTGTG ATCAAGTGCT ACCAGGAACT CCTGGCTGGT 
CAAGATGGTA TTCCTCAGAC CATCACCGAT GTCTACCTCC TCGTCTTACA GCATTTCTTT 
CAGCGAAAGT CATCTCAGCC CCAAAGTGGT CTGGGAAAGG CCTGGTTGGC TGAACATTTG 
GACACAGTGT TAAAGCTCGG AGAGTTGGCA CTAGAAGGTC TGCAAACCTC CTGCTATGTG 
TTCTCAGGTT ATGAGCTACA GAGAAACAGG ATCACAGAGC AAGATGTCGG CATAGGCTTC 
CTCATTTACT GCAGTGACAT ATCTGTGAAT GATTGTAAAC GTTATGAATT TCTGCACATT 
ACCTTACAGT GTTTTTTTGC TGCTCTGTAT GTTATTCTGA ATCGCAACAA TGATCGTTCT 
GCTATCTCCA GACTCTTTCA GCCTCGCAAC AGGCAGGTGT CAGGCTTGTC TCAAAGCTGT 
CTGGGACAAT GTATGGATCA TTCAGTAGAG GAGTCCCATG AGGCAGAGAC AGCAAACCTT 
CAGATTACAG CTCAGTTTGT GTCAGGACTT TTATCCCAGC GGCACAATAA TCTCCTCCTG 
GAGTGCTGTC CTGCTGCTGT GCGTGAACGC AATGTTAAGC AGGTAGTCAA GTCATTGTCC 
AAAAGGATGC AGAGACATTT CAAGTCCATT CCTCGTCCTG TAGAGGGTGA AAAGAAGAGC 
ATGCATGCCA TGCCAAGTTT TGTTTGGCTG ATCAAGTGCA TCTATGAATT GCAAGACAAC 
AGTATTGCCC AAGATGCCAT GGCTAAGTTG GATGTTGAGC ACCTGAAGCT CACCTACTGT 
AACATTGGGC CTGTTGAATG CACTGCGCTG GCATACGTGC TCAAGAACTT GCGCAAACCA 
GTTGGACTAC AGTTGGACAA CAATTCAGTG GGAGATGTGG GTGTGGAGCA GCTTCTGCCG 
TGTCTGCCTA TGTGCCACTC CCTTTACTTA AGGAACAACA ACATATCAGA TGAAGGAATA 
CGAAAACTTC TGGAAAAGGG AATAGAGTGT GAAAACTTCC AGAAAATAGC GCTTTTCAAC 
AACAAGCTCA CTGACACTTG CACCCAATAC TTTTCTTGCC TGCTAAAATC AAAGCAGAAC 
TTCCTGGCTT TGAGGCTTGG AAATAATAAC ATCACATCAG TTGGAGCAGA GCAGCTGGCA 
GAAGGCCTAA GCTACAACCA ATCTTTACAG TTTCTGGGAT TATGGGGTAA TAAAGTTGGG 
GACAGGGGAG CTGAGGTATT GGCGGATGCG CTGACGAATA GCAAAACACT AATATGGTTA 
AGTCTGGTGG ATAATGGTGT GGGCAGTGCC GGTGCATGTG CTCTGGCTAA ATTTATCCGA 
CAGAACAAAA GTCTGGAAGA ATTGTGGTTA AACAAAAACT CCATCTGCAA GGAGGGGGTT 
GATTGTCTGA TAGAGGCACT GAAAATGAAC ACTAGTGTTA AGAAAGTCTG GCTGAGAGGA 
AATAATCTGA GCCCACAGGA AGAAGAGCAG CTGAGCCAGC AAGAATGCAG ATTGACCTTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NOD2 ( XM_009303353.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009301628.1 Danio rerio nucleotide-binding oligomerization domain containing 2 (nod2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa46585
Clone ID Related Accession (Same CDS sequence)	XM_009303353.2
Accession Version	XM_009303353.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2211bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	nucleotide-binding oligomerization domain-containing protein 2 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009303353.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATAGCT CTGGTGAAAC TACAACATTA GGGCTGGAGG ATGTAGTGGG TCCCATGGGG 
ACTCTTAATG AAGACGCTGA CACCGTTCTT GTGTCTGGGG AGGCAGGAAG TGGCAAAAGC 
ACATTAGTTC AGCGACTGCA CTTGCTCTGG GCAAGAGAAG CTTTGCTGCT AAACACTTTT 
CTGCTGTTCC CCTTCAGTTG CCGCAAGCTA AATGCAGAGC ACAGAGAGCT TTCTTTGAAA 
GAGCTCCTCT TCCTGCACTG CTGCTGGCCT GATCGAAACC AGGATGAGGT CTTCCAATTC 
ATCCTAGACC ACCCTCATTT GGTGCTCTTT ACCTTTGACG GACTCGACGA GTTTAAACTG 
GGCTTCACAG ATGAAGAACG TCACTGTTGC CCTACAAAGC AAGTGCCTAT ACCTGTATTG 
CTTTTCAATC TGTTGCAGGG TACCCTAATG AAGGGCGTTA TGAAGGTGGT CACCAGCAGG 
CCACATGCTG TGGGCCCATC ACTGAAGCGC TATCTCCGCA AAGAAGTGCT CCTCAAGGGG 
TTTTCTCCTG GAGGTATTGA CTGCTTTGTT AAGAAACATT ATAGTGACCC TGCAATGGCT 
ACAAGAGTCA TAGAGTCTGT CCAGGGTAAC ACTGCATTAC TAGGTCTTTG CCACATTCCT 
GTCTTCTGCT GGATTGTGAT CAAGTGCTAC CAGGAACTCC TGGCTGGTCA AGATGGTATT 
CCTCAGACCA TCACCGATGT CTACCTCCTC GTCTTACAGC ATTTCTTTCA GCGAAAGTCA 
TCTCAGCCCC AAAGTGGTCT GGGAAAGGCC TGGTTGGCTG AACATTTGGA CACAGTGTTA 
AAGCTCGGAG AGTTGGCACT AGAAGGTCTG CAAACCTCCT GCTATGTGTT CTCAGGTTAT 
GAGCTACAGA GAAACAGGAT CACAGAGCAA GATGTCGGCA TAGGCTTCCT CATTTACTGC 
AGTGACATAT CTGTGAATGA TTGTAAACGT TATGAATTTC TGCACATTAC CTTACAGTGT 
TTTTTTGCTG CTCTGTATGT TATTCTGAAT CGCAACAATG ATCGTTCTGC TATCTCCAGA 
CTCTTTCAGC CTCGCAACAG GCAGGTGTCA GGCTTGTCTC AAAGCTGTCT GGGACAATGT 
ATGGATCATT CAGTAGAGGA GTCCCATGAG GCAGAGACAG CAAACCTTCA GATTACAGCT 
CAGTTTGTGT CAGGACTTTT ATCCCAGCGG CACAATAATC TCCTCCTGGA GTGCTGTCCT 
GCTGCTGTGC GTGAACGCAA TGTTAAGCAG GTAGTCAAGT CATTGTCCAA AAGGATGCAG 
AGACATTTCA AGTCCATTCC TCGTCCTGTA GAGGGTGAAA AGAAGAGCAT GCATGCCATG 
CCAAGTTTTG TTTGGCTGAT CAAGTGCATC TATGAATTGC AAGACAACAG TATTGCCCAA 
GATGCCATGG CTAAGTTGGA TGTTGAGCAC CTGAAGCTCA CCTACTGTAA CATTGGGCCT 
GTTGAATGCA CTGCGCTGGC ATACGTGCTC AAGAACTTGC GCAAACCAGT TGGACTACAG 
TTGGACAACA ATTCAGTGGG AGATGTGGGT GTGGAGCAGC TTCTGCCGTG TCTGCCTATG 
TGCCACTCCC TTTACTTAAG GAACAACAAC ATATCAGATG AAGGAATACG AAAACTTCTG 
GAAAAGGGAA TAGAGTGTGA AAACTTCCAG AAAATAGCGC TTTTCAACAA CAAGCTCACT 
GACACTTGCA CCCAATACTT TTCTTGCCTG CTAAAATCAA AGCAGAACTT CCTGGCTTTG 
AGGCTTGGAA ATAATAACAT CACATCAGTT GGAGCAGAGC AGCTGGCAGA AGGCCTAAGC 
TACAACCAAT CTTTACAGTT TCTGGGATTA TGGGGTAATA AAGTTGGGGA CAGGGGAGCT 
GAGGTATTGG CGGATGCGCT GACGAATAGC AAAACACTAA TATGGTTAAG TCTGGTGGAT 
AATGGTGTGG GCAGTGCCGG TGCATGTGCT CTGGCTAAAT TTATCCGACA GAACAAAAGT 
CTGGAAGAAT TGTGGTTAAA CAAAAACTCC ATCTGCAAGG AGGGGGTTGA TTGTCTGATA 
GAGGCACTGA AAATGAACAC TAGTGTTAAG AAAGTCTGGC TGAGAGGAAA TAATCTGAGC 
CCACAGGAAG AAGAGCAGCT GAGCCAGCAA GAATGCAGAT TGACCTTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009301628.1
CDS	1315..3525
Translation	MNSSGETTTLGLEDVVGPMGTLNEDADTVLVSGEAGSGKSTLVQRLHLLWAREALLLNTFLLFPFSCRKLNAEHRELSLKELLFLHCCWPDRNQDEVFQFILDHPHLVLFTFDGLDEFKLGFTDEERHCCPTKQVPIPVLLFNLLQGTLMKGVMKVVTSRPHAVGPSLKRYLRKEVLLKGFSPGGIDCFVKKHYSDPAMATRVIESVQGNTALLGLCHIPVFCWIVIKCYQELLAGQDGIPQTITDVYLLVLQHFFQRKSSQPQSGLGKAWLAEHLDTVLKLGELALEGLQTSCYVFSGYELQRNRITEQDVGIGFLIYCSDISVNDCKRYEFLHITLQCFFAALYVILNRNNDRSAISRLFQPRNRQVSGLSQSCLGQCMDHSVEESHEAETANLQITAQFVSGLLSQRHNNLLLECCPAAVRERNVKQVVKSLSKRMQRHFKSIPRPVEGEKKSMHAMPSFVWLIKCIYELQDNSIAQDAMAKLDVEHLKLTYCNIGPVECTALAYVLKNLRKPVGLQLDNNSVGDVGVEQLLPCLPMCHSLYLRNNNISDEGIRKLLEKGIECENFQKIALFNNKLTDTCTQYFSCLLKSKQNFLALRLGNNNITSVGAEQLAEGLSYNQSLQFLGLWGNKVGDRGAEVLADALTNSKTLIWLSLVDNGVGSAGACALAKFIRQNKSLEELWLNKNSICKEGVDCLIEALKMNTSVKKVWLRGNNLSPQEEEQLSQQECRLTF

Target ORF information:

RefSeq Version	XM_009303353.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio nucleotide-binding oligomerization domain containing 2 (nod2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009303353.2

ORF Insert Sequence:

ATGAATAGCT CTGGTGAAAC TACAACATTA GGGCTGGAGG ATGTAGTGGG TCCCATGGGG 
ACTCTTAATG AAGACGCTGA CACCGTTCTT GTGTCTGGGG AGGCAGGAAG TGGCAAAAGC 
ACATTAGTTC AGCGACTGCA CTTGCTCTGG GCAAGAGAAG CTTTGCTGCT AAACACTTTT 
CTGCTGTTCC CCTTCAGTTG CCGCAAGCTA AATGCAGAGC ACAGAGAGCT TTCTTTGAAA 
GAGCTCCTCT TCCTGCACTG CTGCTGGCCT GATCGAAACC AGGATGAGGT CTTCCAATTC 
ATCCTAGACC ACCCTCATTT GGTGCTCTTT ACCTTTGACG GACTCGACGA GTTTAAACTG 
GGCTTCACAG ATGAAGAACG TCACTGTTGC CCTACAAAGC AAGTGCCTAT ACCTGTATTG 
CTTTTCAATC TGTTGCAGGG TACCCTAATG AAGGGCGTTA TGAAGGTGGT CACCAGCAGG 
CCACATGCTG TGGGCCCATC ACTGAAGCGC TATCTCCGCA AAGAAGTGCT CCTCAAGGGG 
TTTTCTCCTG GAGGTATTGA CTGCTTTGTT AAGAAACATT ATAGTGACCC TGCAATGGCT 
ACAAGAGTCA TAGAGTCTGT CCAGGGTAAC ACTGCATTAC TAGGTCTTTG CCACATTCCT 
GTCTTCTGCT GGATTGTGAT CAAGTGCTAC CAGGAACTCC TGGCTGGTCA AGATGGTATT 
CCTCAGACCA TCACCGATGT CTACCTCCTC GTCTTACAGC ATTTCTTTCA GCGAAAGTCA 
TCTCAGCCCC AAAGTGGTCT GGGAAAGGCC TGGTTGGCTG AACATTTGGA CACAGTGTTA 
AAGCTCGGAG AGTTGGCACT AGAAGGTCTG CAAACCTCCT GCTATGTGTT CTCAGGTTAT 
GAGCTACAGA GAAACAGGAT CACAGAGCAA GATGTCGGCA TAGGCTTCCT CATTTACTGC 
AGTGACATAT CTGTGAATGA TTGTAAACGT TATGAATTTC TGCACATTAC CTTACAGTGT 
TTTTTTGCTG CTCTGTATGT TATTCTGAAT CGCAACAATG ATCGTTCTGC TATCTCCAGA 
CTCTTTCAGC CTCGCAACAG GCAGGTGTCA GGCTTGTCTC AAAGCTGTCT GGGACAATGT 
ATGGATCATT CAGTAGAGGA GTCCCATGAG GCAGAGACAG CAAACCTTCA GATTACAGCT 
CAGTTTGTGT CAGGACTTTT ATCCCAGCGG CACAATAATC TCCTCCTGGA GTGCTGTCCT 
GCTGCTGTGC GTGAACGCAA TGTTAAGCAG GTAGTCAAGT CATTGTCCAA AAGGATGCAG 
AGACATTTCA AGTCCATTCC TCGTCCTGTA GAGGGTGAAA AGAAGAGCAT GCATGCCATG 
CCAAGTTTTG TTTGGCTGAT CAAGTGCATC TATGAATTGC AAGACAACAG TATTGCCCAA 
GATGCCATGG CTAAGTTGGA TGTTGAGCAC CTGAAGCTCA CCTACTGTAA CATTGGGCCT 
GTTGAATGCA CTGCGCTGGC ATACGTGCTC AAGAACTTGC GCAAACCAGT TGGACTACAG 
TTGGACAACA ATTCAGTGGG AGATGTGGGT GTGGAGCAGC TTCTGCCGTG TCTGCCTATG 
TGCCACTCCC TTTACTTAAG GAACAACAAC ATATCAGATG AAGGAATACG AAAACTTCTG 
GAAAAGGGAA TAGAGTGTGA AAACTTCCAG AAAATAGCGC TTTTCAACAA CAAGCTCACT 
GACACTTGCA CCCAATACTT TTCTTGCCTG CTAAAATCAA AGCAGAACTT CCTGGCTTTG 
AGGCTTGGAA ATAATAACAT CACATCAGTT GGAGCAGAGC AGCTGGCAGA AGGCCTAAGC 
TACAACCAAT CTTTACAGTT TCTGGGATTA TGGGGTAATA AAGTTGGGGA CAGGGGAGCT 
GAGGTATTGG CGGATGCGCT GACGAATAGC AAAACACTAA TATGGTTAAG TCTGGTGGAT 
AATGGTGTGG GCAGTGCCGG TGCATGTGCT CTGGCTAAAT TTATCCGACA GAACAAAAGT 
CTGGAAGAAT TGTGGTTAAA CAAAAACTCC ATCTGCAAGG AGGGGGTTGA TTGTCTGATA 
GAGGCACTGA AAATGAACAC TAGTGTTAAG AAAGTCTGGC TGAGAGGAAA TAATCTGAGC 
CCACAGGAAG AAGAGCAGCT GAGCCAGCAA GAATGCAGAT TGACCTTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Deletion of mstna and mstnb impairs the immune system and affects growth performance in zebrafish.
Fish & shellfish immunology72572-580(2018 Jan)
Wang C,Chen YL,Bian WP,Xie SL,Qi GL,Liu L,Strauss PR,Zou JX,Pei DS

Cell death discovery317067(2017)
Srivastava N,Shelly A,Kumar M,Pant A,Das B,Majumdar T,Mazumder S

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NOD2 cDNA ORF clone, Danio rerio(Zebrafish)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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