plod1a cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	plod1a
Entrez Gene ID	777635
Full Name	procollagen-lysine, 2-oxoglutarate 5-dioxygenase 1a
Synonyms	ik:tdsubs_rzpd609-29b19,plod,plod1,wu:fc29c10,wu:fc95g08,wu:fi18a05,zgc:152876
General protein information	Preferred Names procollagen-lysine, 2-oxoglutarate 5-dioxygenase 1a Names procollagen-lysine,2-oxoglutarate 5-dioxygenase 1 fc95g08 fi18a05 lh1 lysine hydroxylase procollagen lysine 2-oxoglutarate 5-dioxygenase 1 procollagen-lysine 1, 2-oxoglutarate 5-dioxygenase 1a
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001077742.2	NP_001071210.2	procollagen-lysine,2-oxoglutarate 5-dioxygenase 1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	PLOD1	XP_001142788.1
Canis lupus familiaris (dog)	PLOD1	XP_544565.2
Danio rerio (zebrafish)	plod1a	NP_001071210.2
Bos taurus (cattle)	PLOD1	NP_776573.1
Rattus norvegicus (Norway rat)	Plod1	NP_446279.2
Xenopus tropicalis (tropical clawed frog)	plod1	NP_001072340.1
Homo sapiens (human)	PLOD1	NP_000293.2
Mus musculus (house mouse)	Plod1	NP_035252.1
Gallus gallus (chicken)	PLOD1	NP_001005618.1

Gene Ontology
Bibliography

Related articles in PubMed

Comparative transcriptome profiling of the injured zebrafish and mouse hearts identifies miRNA-dependent repair pathways.
Crippa S, Nemir M, Ounzain S, Ibberson M, Berthonneche C, Sarre A, Boisset G, Maison D, Harshman K, Xenarios I, Diviani D, Schorderet D, Pedrazzini T
Cardiovascular research110(1)73-84(2016 May)

Genome-wide mapping of Hif-1? binding sites in zebrafish.
Greenald D, Jeyakani J, Pelster B, Sealy I, Mathavan S, van Eeden FJ
BMC genomics16923(2015 Nov)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Glucocerebrosidase deficiency in zebrafish affects primary bone ossification through increased oxidative stress and reduced Wnt/?-catenin signaling.
Zancan I, Bellesso S, Costa R, Salvalaio M, Stroppiano M, Hammond C, Argenton F, Filocamo M, Moro E
Human molecular genetics24(5)1280-94(2015 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Zebrafish lysyl hydroxylases 1 and 2 were cloned and their patterns of expression during the first two days of embryonic development examined.
Title: Genomic structure and embryonic expression of zebrafish lysyl hydroxylase 1 and lysyl hydroxylase 2.

The following plod1a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the plod1a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa31795	NM_001077742.2 Latest version!	Danio rerio procollagen-lysine, 2-oxoglutarate 5-dioxygenase 1a (plod1a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

plod1a ( NM_001077742.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001071210.2 Danio rerio procollagen-lysine, 2-oxoglutarate 5-dioxygenase 1a (plod1a), mRNA.

Price & Availability↑

CloneID	ODa31795
Clone ID Related Accession (Same CDS sequence)	NM_001077742.2
Accession Version	NM_001077742.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2193bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-07
Organism	Danio rerio(zebrafish)
Product	procollagen-lysine,2-oxoglutarate 5-dioxygenase 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX548250.8. On Mar 26, 2009 this sequence version replaced NM_001077742.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC125830.1, GFIL01030724.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGAGAGGAG TTCTGGCTTT TCTCGCGTGT TTGTTTGCGT CTTTTCCCCC GCTGCACTGT 
AATCAGCAGG GCTCGATCCC CGAGGGAGAT CTTCTGGTGC TCACTGTAGC CACGCAGGAG 
ACTGATGGGT TCAGACGGTT TTTGAGATCT GCTAAACACT TCAATTACAC CATCAAGGTT 
CTCGGACGAG GTGAAACATG GAGAGGTGGA GATTACATGA CAGCTCCAGG TGGAGGACAG 
AAAGTGCGCC TGCTGAAATC TGCACTGGAG GACATCCAGG AAGAGAAAAA AGTCATTCTT 
TTTGTGGATA GCTATGACGT GATTTTTTCG TCTGGTCCCA AAGAGCTGCT GAAGAAGTTT 
CAGCAGGCCA AACACAAAGT AGTGTTCTCC GCCGAGACTC TGATCTGGCC GGACCGTCAC 
CTAGAGGACA AACATCCTCA TGTCAGGGAA GGGAAGAGGT TTCTTGGAGC AGGAGGCTTT 
ATCGGCTATG CAGCAAACCT TAAAAAGATG CTTTCTGACT GGTCAGGAGC TGACGGCGAC 
AGCGACCAGC TGTTTTACAC AAAGATTTAC ATCAACAAAG AGAAAAGAAA GTCTATAAAT 
ATAACTCTGG ACAGCAAGTG CAGATTGTTC CAGAACCTTC ATGGAGCTCT TGACGAGGTC 
GTGCTGAAGT TTGAAGATGG ACGTGTGCGA GCCAGGAATG TGCTTTATGA CACCCTGCCT 
GTTATTATTC ATGGAAACGG ACCCACAAAA CTTCAGATAA ACTACCTAGG GAACTACATC 
CCGAATCTGT GGACATTTGA GACTGGCTGC ACAATGTGTA ATCAGGATCG GCGGCTGCTT 
TCCGGACTGC AGGAGAGTGA ATATCCTGTG GTGGTCATTG GGATCTTCAT CCAACAGCCC 
ACACCCTTCG TCACCGTCTT CTTTGAGCGG TTATTTAATC TGAAATACCC CAAAAACAGA 
CTCAAGCTCT TCATTTATAA TCAGGAAACA CATCATGAAC AACACATACA TGCATTTCTG 
GATTCTCACG AGTCTGAATA CCAGGGTGTG AAGCTGATTG GACCAGAGGA GGACATTGAT 
CCTGTGTCCT CACGTAACCT TGGATTTGAT ATGTGCCGAG AAGACATCGA CTGTGAATAC 
TTCTTCAGCA TAGACGTGGA TGTGGTTTTA AAGAACGAAG ACACACTTAG AATCCTAATT 
GAGCACAACA AGCCTTTTAT TGCACCAATG CTGACCAAAC CGGGGCGACT GTGGACGAAC 
TTCTGGGGTG CTCTCAGTGC AGATGGTTTC TACGCCCGCT CAGAGGATTA CGTGGACATT 
GTTCAGGGAC ACCGCGTTGG TTTGTGGAAT GTGCCATACG TCTCTCACAT ATTCCTGATA 
AAAGCAGACA CGCTGAGGAC TGACCTCAAA GATCCAGACC TGTTCAAGTC CACAACGCTG 
GACCCAGATA TGGCCTTCTG TGAAAAAATA CGAAACAAGG GTGTCTTTAT GTTTGTGACA 
AATATGGATA CTTTCGGTCG AGTTTTATCG ACCGACAATT ACCAAACAAA CCACTTGCAC 
AATGATCTCT GGCAGATCTT TGAAAATCCT GTGGAATGGG AGGAACGATA CATTCATCCA 
AATTATTCCA GAGTTTTAAA AGACGAGTTT ATAGAAACAC CGTGTCCTGA CGTGTACTGG 
TTCCCCATTT TCTCCGAAGT GGCCTGCGAC CATCTTGTTG AGGAGATGGA AAACTTTGGC 
CAGTGGTCAG GAGGAGCAAA TGTGGACAAT AGGATTCAAG GTGGATATGA AAATGTGCCC 
ACAATCGATA TCCACATGAA TCAGGTGGGC TACGAGAAAG AATGGCAGAA GTTTCTTTTG 
GATTACATAG CTCCAGTCAC GGAGAAGATG TTTCCAGGGT ATTACACAAG GGCTCAGTTC 
GATCTGGCGT TTGTTGTTCG TTATAAACCT GATGAGCAGC CTTCATTAAG ACCACATCAC 
GACGCCTCCA CCTTCACCAT CAATATCGCT CTCAATCATG TCGGAATTGA CTTTCAGGGT 
GGAGGCTGTC GGTTCTTGAG GTACGACTGC TCCATTAGGT CTCCCAGGAA GGGCTGGGCT 
TTCATGCACC CGGGACGCCT CACCCATTAC CACGAGGGTC TGCCCACCAC CGAGGGGGTC 
CGATACATCG CCGTCTCATT TGTAGACCCT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001071210.2
CDS	117..2309
Translation	MRGVLAFLACLFASFPPLHCNQQGSIPEGDLLVLTVATQETDGFRRFLRSAKHFNYTIKVLGRGETWRGGDYMTAPGGGQKVRLLKSALEDIQEEKKVILFVDSYDVIFSSGPKELLKKFQQAKHKVVFSAETLIWPDRHLEDKHPHVREGKRFLGAGGFIGYAANLKKMLSDWSGADGDSDQLFYTKIYINKEKRKSINITLDSKCRLFQNLHGALDEVVLKFEDGRVRARNVLYDTLPVIIHGNGPTKLQINYLGNYIPNLWTFETGCTMCNQDRRLLSGLQESEYPVVVIGIFIQQPTPFVTVFFERLFNLKYPKNRLKLFIYNQETHHEQHIHAFLDSHESEYQGVKLIGPEEDIDPVSSRNLGFDMCREDIDCEYFFSIDVDVVLKNEDTLRILIEHNKPFIAPMLTKPGRLWTNFWGALSADGFYARSEDYVDIVQGHRVGLWNVPYVSHIFLIKADTLRTDLKDPDLFKSTTLDPDMAFCEKIRNKGVFMFVTNMDTFGRVLSTDNYQTNHLHNDLWQIFENPVEWEERYIHPNYSRVLKDEFIETPCPDVYWFPIFSEVACDHLVEEMENFGQWSGGANVDNRIQGGYENVPTIDIHMNQVGYEKEWQKFLLDYIAPVTEKMFPGYYTRAQFDLAFVVRYKPDEQPSLRPHHDASTFTINIALNHVGIDFQGGGCRFLRYDCSIRSPRKGWAFMHPGRLTHYHEGLPTTEGVRYIAVSFVDP

Target ORF information:

RefSeq Version	NM_001077742.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio procollagen-lysine, 2-oxoglutarate 5-dioxygenase 1a (plod1a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001077742.2

ORF Insert Sequence:

ATGAGAGGAG TTCTGGCTTT TCTCGCGTGT TTGTTTGCGT CTTTTCCCCC GCTGCACTGT 
AATCAGCAGG GCTCGATCCC CGAGGGAGAT CTTCTGGTGC TCACTGTAGC CACGCAGGAG 
ACTGATGGGT TCAGACGGTT TTTGAGATCT GCTAAACACT TCAATTACAC CATCAAGGTT 
CTCGGACGAG GTGAAACATG GAGAGGTGGA GATTACATGA CAGCTCCAGG TGGAGGACAG 
AAAGTGCGCC TGCTGAAATC TGCACTGGAG GACATCCAGG AAGAGAAAAA AGTCATTCTT 
TTTGTGGATA GCTATGACGT GATTTTTTCG TCTGGTCCCA AAGAGCTGCT GAAGAAGTTT 
CAGCAGGCCA AACACAAAGT AGTGTTCTCC GCCGAGACTC TGATCTGGCC GGACCGTCAC 
CTAGAGGACA AACATCCTCA TGTCAGGGAA GGGAAGAGGT TTCTTGGAGC AGGAGGCTTT 
ATCGGCTATG CAGCAAACCT TAAAAAGATG CTTTCTGACT GGTCAGGAGC TGACGGCGAC 
AGCGACCAGC TGTTTTACAC AAAGATTTAC ATCAACAAAG AGAAAAGAAA GTCTATAAAT 
ATAACTCTGG ACAGCAAGTG CAGATTGTTC CAGAACCTTC ATGGAGCTCT TGACGAGGTC 
GTGCTGAAGT TTGAAGATGG ACGTGTGCGA GCCAGGAATG TGCTTTATGA CACCCTGCCT 
GTTATTATTC ATGGAAACGG ACCCACAAAA CTTCAGATAA ACTACCTAGG GAACTACATC 
CCGAATCTGT GGACATTTGA GACTGGCTGC ACAATGTGTA ATCAGGATCG GCGGCTGCTT 
TCCGGACTGC AGGAGAGTGA ATATCCTGTG GTGGTCATTG GGATCTTCAT CCAACAGCCC 
ACACCCTTCG TCACCGTCTT CTTTGAGCGG TTATTTAATC TGAAATACCC CAAAAACAGA 
CTCAAGCTCT TCATTTATAA TCAGGAAACA CATCATGAAC AACACATACA TGCATTTCTG 
GATTCTCACG AGTCTGAATA CCAGGGTGTG AAGCTGATTG GACCAGAGGA GGACATTGAT 
CCTGTGTCCT CACGTAACCT TGGATTTGAT ATGTGCCGAG AAGACATCGA CTGTGAATAC 
TTCTTCAGCA TAGACGTGGA TGTGGTTTTA AAGAACGAAG ACACACTTAG AATCCTAATT 
GAGCACAACA AGCCTTTTAT TGCACCAATG CTGACCAAAC CGGGGCGACT GTGGACGAAC 
TTCTGGGGTG CTCTCAGTGC AGATGGTTTC TACGCCCGCT CAGAGGATTA CGTGGACATT 
GTTCAGGGAC ACCGCGTTGG TTTGTGGAAT GTGCCATACG TCTCTCACAT ATTCCTGATA 
AAAGCAGACA CGCTGAGGAC TGACCTCAAA GATCCAGACC TGTTCAAGTC CACAACGCTG 
GACCCAGATA TGGCCTTCTG TGAAAAAATA CGAAACAAGG GTGTCTTTAT GTTTGTGACA 
AATATGGATA CTTTCGGTCG AGTTTTATCG ACCGACAATT ACCAAACAAA CCACTTGCAC 
AATGATCTCT GGCAGATCTT TGAAAATCCT GTGGAATGGG AGGAACGATA CATTCATCCA 
AATTATTCCA GAGTTTTAAA AGACGAGTTT ATAGAAACAC CGTGTCCTGA CGTGTACTGG 
TTCCCCATTT TCTCCGAAGT GGCCTGCGAC CATCTTGTTG AGGAGATGGA AAACTTTGGC 
CAGTGGTCAG GAGGAGCAAA TGTGGACAAT AGGATTCAAG GTGGATATGA AAATGTGCCC 
ACAATCGATA TCCACATGAA TCAGGTGGGC TACGAGAAAG AATGGCAGAA GTTTCTTTTG 
GATTACATAG CTCCAGTCAC GGAGAAGATG TTTCCAGGGT ATTACACAAG GGCTCAGTTC 
GATCTGGCGT TTGTTGTTCG TTATAAACCT GATGAGCAGC CTTCATTAAG ACCACATCAC 
GACGCCTCCA CCTTCACCAT CAATATCGCT CTCAATCATG TCGGAATTGA CTTTCAGGGT 
GGAGGCTGTC GGTTCTTGAG GTACGACTGC TCCATTAGGT CTCCCAGGAA GGGCTGGGCT 
TTCATGCACC CGGGACGCCT CACCCATTAC CACGAGGGTC TGCCCACCAC CGAGGGGGTC 
CGATACATCG CCGTCTCATT TGTAGACCCT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Comparative transcriptome profiling of the injured zebrafish and mouse hearts identifies miRNA-dependent repair pathways.
Cardiovascular research110(1)73-84(2016 May)
Crippa S,Nemir M,Ounzain S,Ibberson M,Berthonneche C,Sarre A,Boisset G,Maison D,Harshman K,Xenarios I,Diviani D,Schorderet D,Pedrazzini T

Genome-wide mapping of Hif-1? binding sites in zebrafish.
BMC genomics16923(2015 Nov)
Greenald D,Jeyakani J,Pelster B,Sealy I,Mathavan S,van Eeden FJ

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Glucocerebrosidase deficiency in zebrafish affects primary bone ossification through increased oxidative stress and reduced Wnt/?-catenin signaling.
Human molecular genetics24(5)1280-94(2015 Mar)
Zancan I,Bellesso S,Costa R,Salvalaio M,Stroppiano M,Hammond C,Argenton F,Filocamo M,Moro E

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plod1a cDNA ORF clone, Danio rerio(zebrafish)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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