tpcn2 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	tpcn2
Entrez Gene ID	777614
Full Name	two pore segment channel 2
Synonyms	fi42c05,im:7140344,wu:fi42c05,zgc:152898
General protein information	Preferred Names two pore segment channel 2 Names two pore calcium channel protein 2 voltage-dependent calcium channel protein TPC2
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001077722.1	NP_001071190.1	two pore calcium channel protein 2

Pathways from BioSystems

Homologies

	TPCN2	XP_001101571.1
Bos taurus (cattle)	TPCN2	NP_001179380.1
Danio rerio (zebrafish)	tpcn2	NP_001071190.1
Xenopus tropicalis (tropical clawed frog)	tpcn2	XP_002934116.2
Pan troglodytes (chimpanzee)	TPCN2	XP_001173935.2
Canis lupus familiaris (dog)	TPCN2	XP_540804.3
Mus musculus (house mouse)	Tpcn2	NP_666318.2
Rattus norvegicus (Norway rat)	Tpcn2	NP_001101036.1
Gallus gallus (chicken)	TPCN2	XP_004941585.1
Homo sapiens (human)	TPCN2	NP_620714.2

Gene Ontology
Bibliography

Related articles in PubMed

Ca
Kelu JJ, Webb SE, Parrington J, Galione A, Miller AL
Developmental biology425(2)109-129(2017 05)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

Disruption of CLPB is associated with congenital microcephaly, severe encephalopathy and 3-methylglutaconic aciduria.
Capo-Chichi JM, Boissel S, Brustein E, Pickles S, Fallet-Bianco C, Nassif C, Patry L, Dobrzeniecka S, Liao M, Labuda D, Samuels ME, Hamdan FF, Vande Velde C, Rouleau GA, Drapeau P, Michaud JL
Journal of medical genetics52(5)303-11(2015 May)

Two-Pore Channel 2 activity is required for slow muscle cell-generated Ca(2+) signaling during myogenesis in intact zebrafish.
Kelu JJ, Chan HL, Webb SE, Cheng AH, Ruas M, Parrington J, Galione A, Miller AL
The International journal of developmental biology59(7-9)313-25(2015)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

localized calcium (Ca(2+)) release via TPC2 might trigger the generation of more global Ca(2+) release from the sarcoplasmic reticulum via Ca(2+)-induced Ca(2+) release.
Title: Ca

TPC2-mediated Ca(2+) release from lysosomes may act upstream from RyR- and IP 3R-mediated Ca(2+) release, suggesting that the former might initiate the SR-mediated Ca(2+)-induced-Ca(2+)-release essential for SMC myogenesis and function.
Title: Two-Pore Channel 2 activity is required for slow muscle cell-generated Ca(2+) signaling during myogenesis in intact zebrafish.

The following tpcn2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the tpcn2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa38253	NM_001077722.1 Latest version!	Danio rerio two pore segment channel 2 (tpcn2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

tpcn2 ( NM_001077722.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001071190.1 Danio rerio two pore segment channel 2 (tpcn2), mRNA.

Price & Availability↑

CloneID	ODa38253
Clone ID Related Accession (Same CDS sequence)	NM_001077722.1
Accession Version	NM_001077722.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2325bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-15
Organism	Danio rerio(zebrafish)
Product	two pore calcium channel protein 2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC125833.1. ##Evidence-Data-START## Transcript exon combination :: BC125833.1 [ECO:0000332] ##Evidence-Data-END##

ATGGAAGAAG AACCGCTGCT GGCTGGCAGC ATTAACCAGG GTTCAGGAGA TTATGGAGCT 
CACAGTGAAA GTTGTCTACA TTATCCGGAT GAACACACGC CGAGATCAAG ACGACTGTCC 
TATTCGGTGA CTGACGATTC GTGCAATGTG GAAGAAGATG CTGATGCAGA CCTTTATGTC 
CAGCAGGCAG TAGTATTCAT CGAAGATGCG ATTAAGTATC GATCCATTAA CCACAGGGTG 
GATTCTGGGT CCTTACGATT GTATAGATGG TACTACTCCA ACTTATGTCA GTGGGGTCTG 
GGCTTAACTA TTGCTGTTGT ACTGGCTTTG GCCTTCATTG AGAGACCCTC CTCTTTAACC 
TACACCTCAG ACATTCGTGT CAAACCCAAG CCCTGGGAGC CACCCTGTGG AATGACAGAG 
GGAATCGAGA TCGTCTGCCT TTGTATCTTC ATCCTAGATG TCACCGCAAA GGGTTATCTG 
ATAGGATGGG AGGAGTTTCG CATGAACAAG TGGCTGTTGG CTTATTTAAT AGTGATCACA 
GCATCGGTTA TTGATTGGAT GTTAAGTATT AGCATGTTGT GCGATGAGAA TTTAAGAGTC 
AGGAGACTGA TTCGCCCATT TTTCCTCCTA CAAAACTCTT CCCTTATGAA GAAAACTCTA 
AAATGTATTA AAAGGACCCT TCCCGAGATA GCAAGTGTGA TTCTTCTCCT GGCATTGCAC 
ATCTGCTTAT TCACCATGAT TGGAATGCTG ATCTTTGCCA AATCAGATGA CCCGAAGCAG 
AATGGAGAAT GGCAGACATA CTTCAGAAAC CTTCCCAAAG CTCTTTCTTC TCTGCTAGTG 
CTGCTCACCA CAGCTAATAA CCCTGATGTC ATGATTCCAG CATACTCTCT AAACCGAGGC 
TATTCTATAT TTTTCATACT CTTTAGTGTG TTTGGAACAT ATTTGTTGAT GAATCTAATG 
ACAGCTATTA TTTATAACCA GTTCAGGGGA TATTTACTGA TGTCAGTTCA GACGTCTATC 
ATCAGGAGGC GTCTGGGTAT CCGGGCTGCG TTTGAGGTGC TCTGCTGTCC AGGCCGAGGT 
CACACCAGCA CTCAGGCTGA AGGCCACGTG GAGCGAGTGG CGGTGAGCAT GTTTCTGAAG 
GTCATGGAGA GAGTCCACAT GAAGTCCTAC TGCAGACAAG CTATCGTTAA GGCCGCTCGG 
CGGTTTCCAG ATGGCTTCAT CAGCGGCGAG GACTTCCAGA GGCTCTTCAA TGAGCTGGAC 
AAGGATTTTG TTAAAGAGCA TCCACCAAAG CCTGAGTACA GTTCCTCTGG CCTGCAGCAT 
ATCCAGTATG TCTACAGTCA CTATTATATT TCTGTGCTGG GAAATGCTGT CGCTCTGGCC 
AATGTCATCT GCATCTGTAC TGTTTTGGTT CTGAATGCAG AGAAATCTGC CTCAGAGAAA 
AATTACTTCT ATATGGAGAT CATCAACTGT ATTTTCATCC TTTACTATTT AATAGAGATG 
CTGCTGAAAA TAGTAGCGTT TGGTTGGAAA GGTTACCTGT CATACAGGAA TAACATTTTT 
GATGGATTTC TCACAGTTCT TCTGCTGGCA ATTCAGATCG TCATATTCAT CACATTCAAG 
ATTCCCTATG TGGACGTGGA TCCGGTTCCG CGGCACGTGA TGGCTTTGTG GGAGATGATA 
CGACTGGTCA ACATGTTGAT CGTTTTCCGC TTCCTCAGGA TTATTCCAGA AATCAAGTTG 
ATGGCAGTGG TGGCCAGCAC TATCGTTGAC CTGGTAAAAA ACCTACGGGC ATTTGCTGGG 
ATTCTTCTGG TGGTATACTA TATGTTTGCA GTTCTCGGTA TCTGGCTTTT CCAAGGAGCT 
ATTAGTCCTC CATCAAACAT GAGTCTGGTC TCCAACTCCA GCTTAGAGAA CATCACAGGG 
CCCTATAGCA TGGAATGTGG TACTTTTGAG CAGCTCGAAT ACTGGCCAAA CAACTTTGAT 
GACTTTGCTT CCTCTCTGAT TCTGCTCTAT AACATCATGG TGGTGAATAA CTGGCATGTT 
TTCACAGACG CATATGCCAG ATACACTACA GACTGGTCTC TGGTGTACTT TGTGGTCTGG 
TGGTTGACAT CTTCAGTCAT GTGGGTCAAC CTGTTTGTGG CACTCATTTT AGAGAACTTC 
ACCTATAAGT GGGACCGCAG TAATGGTCTG TCTGTTGAAG ATGTGGAGAG GATCGCCTAT 
CAAAGCACTG TGCAGCTTAT GTTCAAAGAG CACGTCAAAG AGCCGACAGA AGAAGAGTTA 
CTAGCTCAAC TACATCAACA TCCTCATCTC CACCTCTCCT GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001071190.1
CDS	144..2468
Translation	MEEEPLLAGSINQGSGDYGAHSESCLHYPDEHTPRSRRLSYSVTDDSCNVEEDADADLYVQQAVVFIEDAIKYRSINHRVDSGSLRLYRWYYSNLCQWGLGLTIAVVLALAFIERPSSLTYTSDIRVKPKPWEPPCGMTEGIEIVCLCIFILDVTAKGYLIGWEEFRMNKWLLAYLIVITASVIDWMLSISMLCDENLRVRRLIRPFFLLQNSSLMKKTLKCIKRTLPEIASVILLLALHICLFTMIGMLIFAKSDDPKQNGEWQTYFRNLPKALSSLLVLLTTANNPDVMIPAYSLNRGYSIFFILFSVFGTYLLMNLMTAIIYNQFRGYLLMSVQTSIIRRRLGIRAAFEVLCCPGRGHTSTQAEGHVERVAVSMFLKVMERVHMKSYCRQAIVKAARRFPDGFISGEDFQRLFNELDKDFVKEHPPKPEYSSSGLQHIQYVYSHYYISVLGNAVALANVICICTVLVLNAEKSASEKNYFYMEIINCIFILYYLIEMLLKIVAFGWKGYLSYRNNIFDGFLTVLLLAIQIVIFITFKIPYVDVDPVPRHVMALWEMIRLVNMLIVFRFLRIIPEIKLMAVVASTIVDLVKNLRAFAGILLVVYYMFAVLGIWLFQGAISPPSNMSLVSNSSLENITGPYSMECGTFEQLEYWPNNFDDFASSLILLYNIMVVNNWHVFTDAYARYTTDWSLVYFVVWWLTSSVMWVNLFVALILENFTYKWDRSNGLSVEDVERIAYQSTVQLMFKEHVKEPTEEELLAQLHQHPHLHLSW

Target ORF information:

RefSeq Version	NM_001077722.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio two pore segment channel 2 (tpcn2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001077722.1

ORF Insert Sequence:

ATGGAAGAAG AACCGCTGCT GGCTGGCAGC ATTAACCAGG GTTCAGGAGA TTATGGAGCT 
CACAGTGAAA GTTGTCTACA TTATCCGGAT GAACACACGC CGAGATCAAG ACGACTGTCC 
TATTCGGTGA CTGACGATTC GTGCAATGTG GAAGAAGATG CTGATGCAGA CCTTTATGTC 
CAGCAGGCAG TAGTATTCAT CGAAGATGCG ATTAAGTATC GATCCATTAA CCACAGGGTG 
GATTCTGGGT CCTTACGATT GTATAGATGG TACTACTCCA ACTTATGTCA GTGGGGTCTG 
GGCTTAACTA TTGCTGTTGT ACTGGCTTTG GCCTTCATTG AGAGACCCTC CTCTTTAACC 
TACACCTCAG ACATTCGTGT CAAACCCAAG CCCTGGGAGC CACCCTGTGG AATGACAGAG 
GGAATCGAGA TCGTCTGCCT TTGTATCTTC ATCCTAGATG TCACCGCAAA GGGTTATCTG 
ATAGGATGGG AGGAGTTTCG CATGAACAAG TGGCTGTTGG CTTATTTAAT AGTGATCACA 
GCATCGGTTA TTGATTGGAT GTTAAGTATT AGCATGTTGT GCGATGAGAA TTTAAGAGTC 
AGGAGACTGA TTCGCCCATT TTTCCTCCTA CAAAACTCTT CCCTTATGAA GAAAACTCTA 
AAATGTATTA AAAGGACCCT TCCCGAGATA GCAAGTGTGA TTCTTCTCCT GGCATTGCAC 
ATCTGCTTAT TCACCATGAT TGGAATGCTG ATCTTTGCCA AATCAGATGA CCCGAAGCAG 
AATGGAGAAT GGCAGACATA CTTCAGAAAC CTTCCCAAAG CTCTTTCTTC TCTGCTAGTG 
CTGCTCACCA CAGCTAATAA CCCTGATGTC ATGATTCCAG CATACTCTCT AAACCGAGGC 
TATTCTATAT TTTTCATACT CTTTAGTGTG TTTGGAACAT ATTTGTTGAT GAATCTAATG 
ACAGCTATTA TTTATAACCA GTTCAGGGGA TATTTACTGA TGTCAGTTCA GACGTCTATC 
ATCAGGAGGC GTCTGGGTAT CCGGGCTGCG TTTGAGGTGC TCTGCTGTCC AGGCCGAGGT 
CACACCAGCA CTCAGGCTGA AGGCCACGTG GAGCGAGTGG CGGTGAGCAT GTTTCTGAAG 
GTCATGGAGA GAGTCCACAT GAAGTCCTAC TGCAGACAAG CTATCGTTAA GGCCGCTCGG 
CGGTTTCCAG ATGGCTTCAT CAGCGGCGAG GACTTCCAGA GGCTCTTCAA TGAGCTGGAC 
AAGGATTTTG TTAAAGAGCA TCCACCAAAG CCTGAGTACA GTTCCTCTGG CCTGCAGCAT 
ATCCAGTATG TCTACAGTCA CTATTATATT TCTGTGCTGG GAAATGCTGT CGCTCTGGCC 
AATGTCATCT GCATCTGTAC TGTTTTGGTT CTGAATGCAG AGAAATCTGC CTCAGAGAAA 
AATTACTTCT ATATGGAGAT CATCAACTGT ATTTTCATCC TTTACTATTT AATAGAGATG 
CTGCTGAAAA TAGTAGCGTT TGGTTGGAAA GGTTACCTGT CATACAGGAA TAACATTTTT 
GATGGATTTC TCACAGTTCT TCTGCTGGCA ATTCAGATCG TCATATTCAT CACATTCAAG 
ATTCCCTATG TGGACGTGGA TCCGGTTCCG CGGCACGTGA TGGCTTTGTG GGAGATGATA 
CGACTGGTCA ACATGTTGAT CGTTTTCCGC TTCCTCAGGA TTATTCCAGA AATCAAGTTG 
ATGGCAGTGG TGGCCAGCAC TATCGTTGAC CTGGTAAAAA ACCTACGGGC ATTTGCTGGG 
ATTCTTCTGG TGGTATACTA TATGTTTGCA GTTCTCGGTA TCTGGCTTTT CCAAGGAGCT 
ATTAGTCCTC CATCAAACAT GAGTCTGGTC TCCAACTCCA GCTTAGAGAA CATCACAGGG 
CCCTATAGCA TGGAATGTGG TACTTTTGAG CAGCTCGAAT ACTGGCCAAA CAACTTTGAT 
GACTTTGCTT CCTCTCTGAT TCTGCTCTAT AACATCATGG TGGTGAATAA CTGGCATGTT 
TTCACAGACG CATATGCCAG ATACACTACA GACTGGTCTC TGGTGTACTT TGTGGTCTGG 
TGGTTGACAT CTTCAGTCAT GTGGGTCAAC CTGTTTGTGG CACTCATTTT AGAGAACTTC 
ACCTATAAGT GGGACCGCAG TAATGGTCTG TCTGTTGAAG ATGTGGAGAG GATCGCCTAT 
CAAAGCACTG TGCAGCTTAT GTTCAAAGAG CACGTCAAAG AGCCGACAGA AGAAGAGTTA 
CTAGCTCAAC TACATCAACA TCCTCATCTC CACCTCTCCT GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Ca
Developmental biology425(2)109-129(2017 05)
Kelu JJ,Webb SE,Parrington J,Galione A,Miller AL

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

Disruption of CLPB is associated with congenital microcephaly, severe encephalopathy and 3-methylglutaconic aciduria.
Journal of medical genetics52(5)303-11(2015 May)
Capo-Chichi JM,Boissel S,Brustein E,Pickles S,Fallet-Bianco C,Nassif C,Patry L,Dobrzeniecka S,Liao M,Labuda D,Samuels ME,Hamdan FF,Vande Velde C,Rouleau GA,Drapeau P,Michaud JL

Two-Pore Channel 2 activity is required for slow muscle cell-generated Ca(2+) signaling during myogenesis in intact zebrafish.
The International journal of developmental biology59(7-9)313-25(2015)
Kelu JJ,Chan HL,Webb SE,Cheng AH,Ruas M,Parrington J,Galione A,Miller AL

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tpcn2 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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