HSPA12B cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	HSPA12B
Entrez Gene ID	735291
Full Name	heat shock protein 12B
General protein information	Preferred Names heat shock protein 12B Names heat shock 70 kDa protein 12B GA2692 heat shock protein 70 kD 12b
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001042686.1	NP_001036151.1	heat shock 70 kDa protein 12B
XM_009303536.2	XP_009301811.1	heat shock 70 kDa protein 12B isoform X1

Pathways from BioSystems

Homologies

Magnaporthe oryzae (rice blast fungus)	MGG_03039	XP_003720644.1
	HSPA12B	XP_001111342.1
Danio rerio (zebrafish)	hspa12b	NP_001036151.1
Neurospora crassa	NCU00573	XP_964734.2
Homo sapiens (human)	HSPA12B	NP_443202.3
Xenopus tropicalis (tropical clawed frog)	hspa12b	XP_002936323.2
Pan troglodytes (chimpanzee)	HSPA12B	XP_514486.2
Canis lupus familiaris (dog)	HSPA12B	XP_851446.2
Mus musculus (house mouse)	Hspa12b	NP_082582.1
Bos taurus (cattle)	HSPA12B	NP_001095497.1

Gene Ontology
Bibliography

Related articles in PubMed

Evolution of complexity in the zebrafish synapse proteome.
Bay?s ?, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 Mar)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

The zebrafish mutant bumper shows a hyperproliferation of lens epithelial cells and fibre cell degeneration leading to functional blindness.
Schonthaler HB, Franz-Odendaal TA, Hodel C, Gehring I, Geisler R, Schwarz H, Neuhauss SC, Dahm R
Mechanisms of development127(3-4)203-19(2010 Apr)

A novel endothelial-specific heat shock protein HspA12B is required in both zebrafish development and endothelial functions in vitro.
Hu G, Tang J, Zhang B, Lin Y, Hanai J, Galloway J, Bedell V, Bahary N, Han Z, Ramchandran R, Thisse B, Thisse C, Zon LI, Sukhatme VP
Journal of cell science119(Pt 19)4117-26(2006 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

HspA12B is a highly endothelial-cell-specific and plays a significant role in endothelial cells during development and angiogenesis in vitro, partially attributable to modulation of Akt phosphorylation.
Title: A novel endothelial-specific heat shock protein HspA12B is required in both zebrafish development and endothelial functions in vitro.

The following HSPA12B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HSPA12B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa33934	NM_001042686.1 Latest version!	Danio rerio heat shock protein 12B (hspa12b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
ODa33934	XM_009303536.2 Latest version!	Danio rerio heat shock protein 12B (hspa12b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

HSPA12B ( NM_001042686.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> NP_001036151.1 Danio rerio heat shock protein 12B (hspa12b), mRNA.

Price & Availability↑

CloneID	ODa33934
Clone ID Related Accession (Same CDS sequence)	NM_001042686.1 , XM_009303536.2
Accession Version	NM_001042686.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2076bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-26
Organism	Danio rerio(zebrafish)
Product	heat shock 70 kDa protein 12B
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from DQ119052.1. ##Evidence-Data-START## Transcript exon combination :: DQ119052.1, BC162264.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGGCAGACG TCTTGCAACT CAGTATCAAC AGTCTACAGG TGCCAGGTGA AGATAAATCA 
GACTCTACAT CTCCATCGGG TTCTCCATTT CCTTCCAGAA ATGAGTGTAG TATCACTCCT 
CTTACTCCCT CCCCCTCTCC GAGAACAGAA GTTAGACCAC GTTTGGCTCG TCCATTTTAT 
GTGGTGGTCG CAATAGATTT TGGCACCACC TCCAGTGGCT ATGCCTTCAG TTTCATTGAA 
GACCCTGAGA CCATTCACAT GATGAGAAGG TGGGAGGGAG GAGACCCTGG TGTAGCAAAT 
CAGAAAAGTC CAACCTGTCT TCTGCTGACC CCTGACCTAC GTTTCCACAG TTTTGGCTTT 
GCAGCGCGAG ACAGTTATCA TGACCTTGAC CCCGAGGAGG CCAGACATTG GCTATACTTT 
GACAAATTCA AAATGAAAAT CCACAGCACA AGTGATCTTA CTATGGAGAC TGAGCTGGAG 
TCTGTTAGTG GAAGAAGAGT GCAGGCCATT GAGGTGTTTG CTCATGCCTT GAGGTTCTTT 
CGAGAACATG CTTTAAAGGA GGTGAAGGAC CAGTCCTCCT CTGTGCTGGA AGGTAATGAA 
GTACGGTGGG TCATCACAGT TCCTGCCGTG TGGAGGCAGC CAGCCAAACA GTTCATGCGA 
GAAGCTGCAT ATCTGGCAGG CTTAGTTCCT CCAGATTCTC CTGAGCAGCT TCTTATAGCT 
CTTGAACCAG AGGCTGCATC AATCTACTGC AGGAAACTGC GTCTTCACCA GGTCACTGAC 
CTAAGCCAGC GTCCAGTAAC TAATGGTTTT GATATAGACG GGTCCCGTCC ATTTGACTCC 
AGTTTTAGGC AAGCCCGTGA GCAGCTCCGC AGAGCCAGAC ATAGCCGAAC TTTTCTGGTA 
GAGAGTGGTA CTGGAGAATT GTGGTCTGAG ATGCAGACAG GTGACCGGTA CATTGTAGCT 
GACTGTGGAG GTGGGACTGT GGATTTGACA GTGCATCAGA TTGAGCAGCC ACAGGGCACT 
CTGAAAGAGC TTTACAAAGC CTCAGGGGGT CCTTATGGTG CAGTTGGTGT GGATCTTGCC 
TTTGAGACAA TGTTGTGCCA AATCTTTGGC ACTGACTTTA TTGACAGTTT TAAAGCTAAA 
CGTCCAGCTG CTTGGGTAGA TCTCACTATT GCATTTGAAG CCCGCAAACG TACAGCAGCT 
CCTGGCCGTG CCAACACTTT AAACATCTCA CTGCCCTTCT CCTTCATTGA CTTCTACAAG 
CAACACCGGG GCCAGAGTGT GGAAACTGCC CTGCGCAAGA GCAATATGAA TTTTATAAAG 
TGGTCATCTC AAGGAATGCT GAGACTCTCT ACGGAGGCCA CGAACGAGCT TTTTCAGCCG 
ACCATTAACA ATATCATAAA ACATATTGAG AACGTCATGC AGAAGGAAGA AGTTAAGGGT 
GTCCGTTTTC TCTTCCTGGT TGGAGGATTC GCAGAGTCAC CCATGCTTCA ACGGGCAATT 
CAGAATACAC TAGGACGGAA CTGCCGTATA ATAATTCCAC ATGATGTAGG TCTAACCATC 
TTGAAGGGTG CAGTCCTTTT TGGTCTAGAC CCCACTGTTG TCAGAGTACG CCGTTGTCCG 
TTAACTTATG GTGTTGGGGT TTTGAACCGC TTTGTAGAAG GAAGACACCC TCATGATAAG 
CTACTCATCA AGGATGGAAG AGAATGGTGC ACTGACATTC TGGACCGCTT TGTAAGTGTT 
GATCAATCTG TGGCCTTGGG TGAGGTTGTG AGGCGGAGCT ACACTCCTGC TAGGATGGGT 
CAACGGAAGA TCATCATCAA CATCTACTGC AGCGACACTG ATGACATAAC CTATATTACC 
GACCCTGGGG TGAGGAAGTG TGGTGCCATC ACGCTAGACC TACTTGAATC AGGGGAAGCT 
TCAGCTAGCA CTGGTGATAA CGATAAAGGA TCAGCATTTG AACGCAGGGA GATTCGCACT 
ACCATGCAGT TTGGTGACAC TGAGATCAAA GTCACAGCAG TTGATGTGGC AACTGGCCGA 
CTAGTGCGGG CATCAATTGA CTTCTTGTCT AACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001036151.1
CDS	267..2342
Translation	MADVLQLSINSLQVPGEDKSDSTSPSGSPFPSRNECSITPLTPSPSPRTEVRPRLARPFYVVVAIDFGTTSSGYAFSFIEDPETIHMMRRWEGGDPGVANQKSPTCLLLTPDLRFHSFGFAARDSYHDLDPEEARHWLYFDKFKMKIHSTSDLTMETELESVSGRRVQAIEVFAHALRFFREHALKEVKDQSSSVLEGNEVRWVITVPAVWRQPAKQFMREAAYLAGLVPPDSPEQLLIALEPEAASIYCRKLRLHQVTDLSQRPVTNGFDIDGSRPFDSSFRQAREQLRRARHSRTFLVESGTGELWSEMQTGDRYIVADCGGGTVDLTVHQIEQPQGTLKELYKASGGPYGAVGVDLAFETMLCQIFGTDFIDSFKAKRPAAWVDLTIAFEARKRTAAPGRANTLNISLPFSFIDFYKQHRGQSVETALRKSNMNFIKWSSQGMLRLSTEATNELFQPTINNIIKHIENVMQKEEVKGVRFLFLVGGFAESPMLQRAIQNTLGRNCRIIIPHDVGLTILKGAVLFGLDPTVVRVRRCPLTYGVGVLNRFVEGRHPHDKLLIKDGREWCTDILDRFVSVDQSVALGEVVRRSYTPARMGQRKIIINIYCSDTDDITYITDPGVRKCGAITLDLLESGEASASTGDNDKGSAFERREIRTTMQFGDTEIKVTAVDVATGRLVRASIDFLSN

Target ORF information:

RefSeq Version	NM_001042686.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio heat shock protein 12B (hspa12b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001042686.1

ORF Insert Sequence:

ATGGCAGACG TCTTGCAACT CAGTATCAAC AGTCTACAGG TGCCAGGTGA AGATAAATCA 
GACTCTACAT CTCCATCGGG TTCTCCATTT CCTTCCAGAA ATGAGTGTAG TATCACTCCT 
CTTACTCCCT CCCCCTCTCC GAGAACAGAA GTTAGACCAC GTTTGGCTCG TCCATTTTAT 
GTGGTGGTCG CAATAGATTT TGGCACCACC TCCAGTGGCT ATGCCTTCAG TTTCATTGAA 
GACCCTGAGA CCATTCACAT GATGAGAAGG TGGGAGGGAG GAGACCCTGG TGTAGCAAAT 
CAGAAAAGTC CAACCTGTCT TCTGCTGACC CCTGACCTAC GTTTCCACAG TTTTGGCTTT 
GCAGCGCGAG ACAGTTATCA TGACCTTGAC CCCGAGGAGG CCAGACATTG GCTATACTTT 
GACAAATTCA AAATGAAAAT CCACAGCACA AGTGATCTTA CTATGGAGAC TGAGCTGGAG 
TCTGTTAGTG GAAGAAGAGT GCAGGCCATT GAGGTGTTTG CTCATGCCTT GAGGTTCTTT 
CGAGAACATG CTTTAAAGGA GGTGAAGGAC CAGTCCTCCT CTGTGCTGGA AGGTAATGAA 
GTACGGTGGG TCATCACAGT TCCTGCCGTG TGGAGGCAGC CAGCCAAACA GTTCATGCGA 
GAAGCTGCAT ATCTGGCAGG CTTAGTTCCT CCAGATTCTC CTGAGCAGCT TCTTATAGCT 
CTTGAACCAG AGGCTGCATC AATCTACTGC AGGAAACTGC GTCTTCACCA GGTCACTGAC 
CTAAGCCAGC GTCCAGTAAC TAATGGTTTT GATATAGACG GGTCCCGTCC ATTTGACTCC 
AGTTTTAGGC AAGCCCGTGA GCAGCTCCGC AGAGCCAGAC ATAGCCGAAC TTTTCTGGTA 
GAGAGTGGTA CTGGAGAATT GTGGTCTGAG ATGCAGACAG GTGACCGGTA CATTGTAGCT 
GACTGTGGAG GTGGGACTGT GGATTTGACA GTGCATCAGA TTGAGCAGCC ACAGGGCACT 
CTGAAAGAGC TTTACAAAGC CTCAGGGGGT CCTTATGGTG CAGTTGGTGT GGATCTTGCC 
TTTGAGACAA TGTTGTGCCA AATCTTTGGC ACTGACTTTA TTGACAGTTT TAAAGCTAAA 
CGTCCAGCTG CTTGGGTAGA TCTCACTATT GCATTTGAAG CCCGCAAACG TACAGCAGCT 
CCTGGCCGTG CCAACACTTT AAACATCTCA CTGCCCTTCT CCTTCATTGA CTTCTACAAG 
CAACACCGGG GCCAGAGTGT GGAAACTGCC CTGCGCAAGA GCAATATGAA TTTTATAAAG 
TGGTCATCTC AAGGAATGCT GAGACTCTCT ACGGAGGCCA CGAACGAGCT TTTTCAGCCG 
ACCATTAACA ATATCATAAA ACATATTGAG AACGTCATGC AGAAGGAAGA AGTTAAGGGT 
GTCCGTTTTC TCTTCCTGGT TGGAGGATTC GCAGAGTCAC CCATGCTTCA ACGGGCAATT 
CAGAATACAC TAGGACGGAA CTGCCGTATA ATAATTCCAC ATGATGTAGG TCTAACCATC 
TTGAAGGGTG CAGTCCTTTT TGGTCTAGAC CCCACTGTTG TCAGAGTACG CCGTTGTCCG 
TTAACTTATG GTGTTGGGGT TTTGAACCGC TTTGTAGAAG GAAGACACCC TCATGATAAG 
CTACTCATCA AGGATGGAAG AGAATGGTGC ACTGACATTC TGGACCGCTT TGTAAGTGTT 
GATCAATCTG TGGCCTTGGG TGAGGTTGTG AGGCGGAGCT ACACTCCTGC TAGGATGGGT 
CAACGGAAGA TCATCATCAA CATCTACTGC AGCGACACTG ATGACATAAC CTATATTACC 
GACCCTGGGG TGAGGAAGTG TGGTGCCATC ACGCTAGACC TACTTGAATC AGGGGAAGCT 
TCAGCTAGCA CTGGTGATAA CGATAAAGGA TCAGCATTTG AACGCAGGGA GATTCGCACT 
ACCATGCAGT TTGGTGACAC TGAGATCAAA GTCACAGCAG TTGATGTGGC AACTGGCCGA 
CTAGTGCGGG CATCAATTGA CTTCTTGTCT AACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HSPA12B ( XM_009303536.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009301811.1 Danio rerio heat shock protein 12B (hspa12b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa33934
Clone ID Related Accession (Same CDS sequence)	NM_001042686.1 , XM_009303536.2
Accession Version	XM_009303536.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2076bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	heat shock 70 kDa protein 12B isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009303536.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGACG TCTTGCAACT CAGTATCAAC AGTCTACAGG TGCCAGGTGA AGATAAATCA 
GACTCTACAT CTCCATCGGG TTCTCCATTT CCTTCCAGAA ATGAGTGTAG TATCACTCCT 
CTTACTCCCT CCCCCTCTCC GAGAACAGAA GTTAGACCAC GTTTGGCTCG TCCATTTTAT 
GTGGTGGTCG CAATAGATTT TGGCACCACC TCCAGTGGCT ATGCCTTCAG TTTCATTGAA 
GACCCTGAGA CCATTCACAT GATGAGAAGG TGGGAGGGAG GAGACCCTGG TGTAGCAAAT 
CAGAAAAGTC CAACCTGTCT TCTGCTGACC CCTGACCTAC GTTTCCACAG TTTTGGCTTT 
GCAGCGCGAG ACAGTTATCA TGACCTTGAC CCCGAGGAGG CCAGACATTG GCTATACTTT 
GACAAATTCA AAATGAAAAT CCACAGCACA AGTGATCTTA CTATGGAGAC TGAGCTGGAG 
TCTGTTAGTG GAAGAAGAGT GCAGGCCATT GAGGTGTTTG CTCATGCCTT GAGGTTCTTT 
CGAGAACATG CTTTAAAGGA GGTGAAGGAC CAGTCCTCCT CTGTGCTGGA AGGTAATGAA 
GTACGGTGGG TCATCACAGT TCCTGCCGTG TGGAGGCAGC CAGCCAAACA GTTCATGCGA 
GAAGCTGCAT ATCTGGCAGG CTTAGTTCCT CCAGATTCTC CTGAGCAGCT TCTTATAGCT 
CTTGAACCAG AGGCTGCATC AATCTACTGC AGGAAACTGC GTCTTCACCA GGTCACTGAC 
CTAAGCCAGC GTCCAGTAAC TAATGGTTTT GATATAGACG GGTCCCGTCC ATTTGACTCC 
AGTTTTAGGC AAGCCCGTGA GCAGCTCCGC AGAGCCAGAC ATAGCCGAAC TTTTCTGGTA 
GAGAGTGGTA CTGGAGAATT GTGGTCTGAG ATGCAGACAG GTGACCGGTA CATTGTAGCT 
GACTGTGGAG GTGGGACTGT GGATTTGACA GTGCATCAGA TTGAGCAGCC ACAGGGCACT 
CTGAAAGAGC TTTACAAAGC CTCAGGGGGT CCTTATGGTG CAGTTGGTGT GGATCTTGCC 
TTTGAGACAA TGTTGTGCCA AATCTTTGGC ACTGACTTTA TTGACAGTTT TAAAGCTAAA 
CGTCCAGCTG CTTGGGTAGA TCTCACTATT GCATTTGAAG CCCGCAAACG TACAGCAGCT 
CCTGGCCGTG CCAACACTTT AAACATCTCA CTGCCCTTCT CCTTCATTGA CTTCTACAAG 
CAACACCGGG GCCAGAGTGT GGAAACTGCC CTGCGCAAGA GCAATATGAA TTTTATAAAG 
TGGTCATCTC AAGGAATGCT GAGACTCTCT ACGGAGGCCA CGAACGAGCT TTTTCAGCCG 
ACCATTAACA ATATCATAAA ACATATTGAG AACGTCATGC AGAAGGAAGA AGTTAAGGGT 
GTCCGTTTTC TCTTCCTGGT TGGAGGATTC GCAGAGTCAC CCATGCTTCA ACGGGCAATT 
CAGAATACAC TAGGACGGAA CTGCCGTATA ATAATTCCAC ATGATGTAGG TCTAACCATC 
TTGAAGGGTG CAGTCCTTTT TGGTCTAGAC CCCACTGTTG TCAGAGTACG CCGTTGTCCG 
TTAACTTATG GTGTTGGGGT TTTGAACCGC TTTGTAGAAG GAAGACACCC TCATGATAAG 
CTACTCATCA AGGATGGAAG AGAATGGTGC ACTGACATTC TGGACCGCTT TGTAAGTGTT 
GATCAATCTG TGGCCTTGGG TGAGGTTGTG AGGCGGAGCT ACACTCCTGC TAGGATGGGT 
CAACGGAAGA TCATCATCAA CATCTACTGC AGCGACACTG ATGACATAAC CTATATTACC 
GACCCTGGGG TGAGGAAGTG TGGTGCCATC ACGCTAGACC TACTTGAATC AGGGGAAGCT 
TCAGCTAGCA CTGGTGATAA CGATAAAGGA TCAGCATTTG AACGCAGGGA GATTCGCACT 
ACCATGCAGT TTGGTGACAC TGAGATCAAA GTCACAGCAG TTGATGTGGC AACTGGCCGA 
CTAGTGCGGG CATCAATTGA CTTCTTGTCT AACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009301811.1
CDS	431..2506
Translation	MADVLQLSINSLQVPGEDKSDSTSPSGSPFPSRNECSITPLTPSPSPRTEVRPRLARPFYVVVAIDFGTTSSGYAFSFIEDPETIHMMRRWEGGDPGVANQKSPTCLLLTPDLRFHSFGFAARDSYHDLDPEEARHWLYFDKFKMKIHSTSDLTMETELESVSGRRVQAIEVFAHALRFFREHALKEVKDQSSSVLEGNEVRWVITVPAVWRQPAKQFMREAAYLAGLVPPDSPEQLLIALEPEAASIYCRKLRLHQVTDLSQRPVTNGFDIDGSRPFDSSFRQAREQLRRARHSRTFLVESGTGELWSEMQTGDRYIVADCGGGTVDLTVHQIEQPQGTLKELYKASGGPYGAVGVDLAFETMLCQIFGTDFIDSFKAKRPAAWVDLTIAFEARKRTAAPGRANTLNISLPFSFIDFYKQHRGQSVETALRKSNMNFIKWSSQGMLRLSTEATNELFQPTINNIIKHIENVMQKEEVKGVRFLFLVGGFAESPMLQRAIQNTLGRNCRIIIPHDVGLTILKGAVLFGLDPTVVRVRRCPLTYGVGVLNRFVEGRHPHDKLLIKDGREWCTDILDRFVSVDQSVALGEVVRRSYTPARMGQRKIIINIYCSDTDDITYITDPGVRKCGAITLDLLESGEASASTGDNDKGSAFERREIRTTMQFGDTEIKVTAVDVATGRLVRASIDFLSN

Target ORF information:

RefSeq Version	XM_009303536.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio heat shock protein 12B (hspa12b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009303536.2

ORF Insert Sequence:

ATGGCAGACG TCTTGCAACT CAGTATCAAC AGTCTACAGG TGCCAGGTGA AGATAAATCA 
GACTCTACAT CTCCATCGGG TTCTCCATTT CCTTCCAGAA ATGAGTGTAG TATCACTCCT 
CTTACTCCCT CCCCCTCTCC GAGAACAGAA GTTAGACCAC GTTTGGCTCG TCCATTTTAT 
GTGGTGGTCG CAATAGATTT TGGCACCACC TCCAGTGGCT ATGCCTTCAG TTTCATTGAA 
GACCCTGAGA CCATTCACAT GATGAGAAGG TGGGAGGGAG GAGACCCTGG TGTAGCAAAT 
CAGAAAAGTC CAACCTGTCT TCTGCTGACC CCTGACCTAC GTTTCCACAG TTTTGGCTTT 
GCAGCGCGAG ACAGTTATCA TGACCTTGAC CCCGAGGAGG CCAGACATTG GCTATACTTT 
GACAAATTCA AAATGAAAAT CCACAGCACA AGTGATCTTA CTATGGAGAC TGAGCTGGAG 
TCTGTTAGTG GAAGAAGAGT GCAGGCCATT GAGGTGTTTG CTCATGCCTT GAGGTTCTTT 
CGAGAACATG CTTTAAAGGA GGTGAAGGAC CAGTCCTCCT CTGTGCTGGA AGGTAATGAA 
GTACGGTGGG TCATCACAGT TCCTGCCGTG TGGAGGCAGC CAGCCAAACA GTTCATGCGA 
GAAGCTGCAT ATCTGGCAGG CTTAGTTCCT CCAGATTCTC CTGAGCAGCT TCTTATAGCT 
CTTGAACCAG AGGCTGCATC AATCTACTGC AGGAAACTGC GTCTTCACCA GGTCACTGAC 
CTAAGCCAGC GTCCAGTAAC TAATGGTTTT GATATAGACG GGTCCCGTCC ATTTGACTCC 
AGTTTTAGGC AAGCCCGTGA GCAGCTCCGC AGAGCCAGAC ATAGCCGAAC TTTTCTGGTA 
GAGAGTGGTA CTGGAGAATT GTGGTCTGAG ATGCAGACAG GTGACCGGTA CATTGTAGCT 
GACTGTGGAG GTGGGACTGT GGATTTGACA GTGCATCAGA TTGAGCAGCC ACAGGGCACT 
CTGAAAGAGC TTTACAAAGC CTCAGGGGGT CCTTATGGTG CAGTTGGTGT GGATCTTGCC 
TTTGAGACAA TGTTGTGCCA AATCTTTGGC ACTGACTTTA TTGACAGTTT TAAAGCTAAA 
CGTCCAGCTG CTTGGGTAGA TCTCACTATT GCATTTGAAG CCCGCAAACG TACAGCAGCT 
CCTGGCCGTG CCAACACTTT AAACATCTCA CTGCCCTTCT CCTTCATTGA CTTCTACAAG 
CAACACCGGG GCCAGAGTGT GGAAACTGCC CTGCGCAAGA GCAATATGAA TTTTATAAAG 
TGGTCATCTC AAGGAATGCT GAGACTCTCT ACGGAGGCCA CGAACGAGCT TTTTCAGCCG 
ACCATTAACA ATATCATAAA ACATATTGAG AACGTCATGC AGAAGGAAGA AGTTAAGGGT 
GTCCGTTTTC TCTTCCTGGT TGGAGGATTC GCAGAGTCAC CCATGCTTCA ACGGGCAATT 
CAGAATACAC TAGGACGGAA CTGCCGTATA ATAATTCCAC ATGATGTAGG TCTAACCATC 
TTGAAGGGTG CAGTCCTTTT TGGTCTAGAC CCCACTGTTG TCAGAGTACG CCGTTGTCCG 
TTAACTTATG GTGTTGGGGT TTTGAACCGC TTTGTAGAAG GAAGACACCC TCATGATAAG 
CTACTCATCA AGGATGGAAG AGAATGGTGC ACTGACATTC TGGACCGCTT TGTAAGTGTT 
GATCAATCTG TGGCCTTGGG TGAGGTTGTG AGGCGGAGCT ACACTCCTGC TAGGATGGGT 
CAACGGAAGA TCATCATCAA CATCTACTGC AGCGACACTG ATGACATAAC CTATATTACC 
GACCCTGGGG TGAGGAAGTG TGGTGCCATC ACGCTAGACC TACTTGAATC AGGGGAAGCT 
TCAGCTAGCA CTGGTGATAA CGATAAAGGA TCAGCATTTG AACGCAGGGA GATTCGCACT 
ACCATGCAGT TTGGTGACAC TGAGATCAAA GTCACAGCAG TTGATGTGGC AACTGGCCGA 
CTAGTGCGGG CATCAATTGA CTTCTTGTCT AACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 Mar)
Bay?s ?,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

The zebrafish mutant bumper shows a hyperproliferation of lens epithelial cells and fibre cell degeneration leading to functional blindness.
Mechanisms of development127(3-4)203-19(2010 Apr)
Schonthaler HB,Franz-Odendaal TA,Hodel C,Gehring I,Geisler R,Schwarz H,Neuhauss SC,Dahm R

A novel endothelial-specific heat shock protein HspA12B is required in both zebrafish development and endothelial functions in vitro.
Journal of cell science119(Pt 19)4117-26(2006 Oct)
Hu G,Tang J,Zhang B,Lin Y,Hanai J,Galloway J,Bedell V,Bahary N,Han Z,Ramchandran R,Thisse B,Thisse C,Zon LI,Sukhatme VP

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HSPA12B cDNA ORF clone, Danio rerio(Zebrafish)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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