mgat5 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	mgat5
Entrez Gene ID	724006
Full Name	mannosyl (alpha-1,6-)-glycoprotein beta-1,6-N-acetyl-glucosaminyltransferase
Synonyms	mgat5a,si:dkeyp-4h4.2,zgc:136939
General protein information	Preferred Names mannosyl (alpha-1,6-)-glycoprotein beta-1,6-N-acetyl-glucosaminyltransferase Names alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase A mannoside acetylglucosaminyltransferase 5
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001045311.1	NP_001038776.1	alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase A

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	MGAT5	XP_515802.2
Danio rerio (zebrafish)	mgat5	NP_001038776.1
Xenopus tropicalis (tropical clawed frog)	mgat5	NP_001120456.1
Homo sapiens (human)	MGAT5	NP_002401.1
Bos taurus (cattle)	MGAT5	NP_001178271.1
Gallus gallus (chicken)	MGAT5	XP_422131.2
Caenorhabditis elegans (roundworm)	gly-2	NP_491874.1
Canis lupus familiaris (dog)	MGAT5	XP_005631994.1
Rattus norvegicus (Norway rat)	Mgat5	NP_075583.1
Mus musculus (house mouse)	Mgat5	NP_660110.2

Gene Ontology
Bibliography

Related articles in PubMed

Loss of Mgat5a-mediated
Pei W, Huang SC, Xu L, Pettie K, Ceci ML, S?nchez M, Allende ML, Burgess SM
Cell regeneration (London, England)53(2016)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

High-throughput gene targeting and phenotyping in zebrafish using CRISPR/Cas9.
Varshney GK, Pei W, LaFave MC, Idol J, Xu L, Gallardo V, Carrington B, Bishop K, Jones M, Li M, Harper U, Huang SC, Prakash A, Chen W, Sood R, Ledin J, Burgess SM
Genome research25(7)1030-42(2015 Jul)

In vivo protein trapping produces a functional expression codex of the vertebrate proteome.
Clark KJ, Balciunas D, Pogoda HM, Ding Y, Westcot SE, Bedell VM, Greenwood TM, Urban MD, Skuster KJ, Petzold AM, Ni J, Nielsen AL, Patowary A, Scaria V, Sivasubbu S, Xu X, Hammerschmidt M, Ekker SC
Nature methods8(6)506-15(2011 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following mgat5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the mgat5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa02936	NM_001045311.1 Latest version!	Danio rerio alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase (mgat5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

mgat5 ( NM_001045311.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001038776.1 Danio rerio alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase (mgat5), mRNA.

Price & Availability↑

CloneID	ODa02936
Clone ID Related Accession (Same CDS sequence)	NM_001045311.1
Accession Version	NM_001045311.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2229bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase A
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC117664.1. ##Evidence-Data-START## Transcript exon combination :: BC117664.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGGGCTCCG TGTACTTGTG GAAGTTGTCT TCCCAAAAGT TGGGTTTCTT CCTCGTGAGT 
TTCGGCTTCA TTTGGGGGAT GATGCTGCTT CATTTCACCA TCCAGCAGAG GGCGACGCAT 
GAAAGCAGCG CGCAGCTACG AATGCAGATA CTGGACCTTA GCAAGCGATA CATTAAAGCC 
CTTGCAGAGG AGAACCAAAG CGTGATGGAT GGACCCTACG TAGGCACCAT GACAGCATAT 
GATCTGAAGA AGACTCTAGC TGTTCTTCTG GACAACATAA TGCAGAGATT GTCCAAGCTG 
GAGTCTAAAG TGGACAATAT CATGTACAAT GGCACCAGCG CCAACCTGAC CAACGGCACC 
GGGACTCCCG GACCAAACCC AGTCAACCCT GAGAAAGTCA ACGTGGCAGA TTTGCTAAAC 
GGAGCGCAGG AAAAATGTGA ACTGCCCTCG CTTGACGGGT TTCCACATTG TGAAGGCAAG 
ATAAAGTGGA TGAAGGACAT GTGGCGTTCG GACCCCTGCT ACGGCAACTA TGGTGTGGAT 
GGGTCCACCT GCTCATTCTT CATTTATTTG AGCGAGGTTG AAAACTGGTG CCCTCGTCTG 
CCGTGGAGGA TTAAGAACAT TGGGGATGAC TTGGACAGGA AAACACAGTT GGAGGTCCGC 
ACAAGTTTTG ACGAGTTGTA TCGCGTGATG TCGCGGCGTG AGGAGTTTCG CTGGATGATG 
CTGCGGATCA AGCGGATGGA GGAGCCGTGG GTCAGCGCCG TTCGCTCACT TGCCAACAAA 
CAAAACCTCC ACCACCGCAA ACGCAAGAAG ATCCTGGTTC ACCTGGGCTT GTTGACGAAG 
GAGTCTGGTT TTAAGATAGC GGAGAATGCG TTCAGCGGCG GTCCTCTGGG TGAGCTGGTG 
CAGTGGAGCG ACCTCATCAC CACACTCTAC CTGCTCGGAC ACGACATCCG AATTTCAGCC 
TCCCTCGCTG AGCTCAAAGA GATCATGAAG AAGGTGATGG ACAATAAATC CAGCTGCCCG 
ACCAAAGGCG ATAAAGTGGT GGAGCTGATT TACATCGACA TCGTGGGCCT GGCGCAGTTC 
AAGAAGACCC TCGGCCCGTC CTGGGTCCAT TATCAGTGTA TGCTGCGTGT GCTGGACTCA 
TTCGGGACGG AGCCGGAGTT CAATCACGCT CACTACGCCC AGTCCAAAGG CCACAAGACT 
CCGTGGGGCA AGTGGAACTT GAACCCGCAG CAGTTCAACA CCATGTTTCC TCACACTCCC 
GATAACAGTT TCCTGGGCTT CGTGGTTGAG CAGCATCTGA ACGCCAGTGA CGTCAAACAC 
ATCGACGACA TCAAGCGGCA AAACCAATCG CTGGTTTACG GAAAAGTGGA CAACTTCTGG 
AAGGATAAAA AGAAGTACCT AGACATTATT CACTCCTATA TGGAAGTTCA CGGCACAGTC 
CATGGCACTA GTACAGTCCA CCTGCCTGGC TACGTGAAGA ACCACGGCAT CCTGAGCGGA 
CGAGACCTGC AGTTCCTGCT GCGAGAGACC AAGCTTTTTG TTGGCCTGTC GTTTCCCTAT 
GAAGGCCCCG CCCCTCTCGA GGCGATAGCC AATGGCTGTG CATTCCTGAA CCCGAGGTTT 
GATCCTGCTA AGAGCAGCAA GAACACCGAT TTCTTCAAAG GCAAACCCAC ACTCAGAGAG 
TTGACGTCTC AGCATCCGTA TGCTGAGGTT TACATCGGCC GGCCGCACGT CTGGACCGTT 
AACATTGAAG ATCCGGCCGA GGTGGAGAAC GCCATTAAAG CCATACTGAG CCAGAAGATA 
GAGCCGTATC TGCCGTATGA GTTCACCTGT GAAGGGATGC TGCAGCGGGT CAATGCTTTC 
ATTGAGAACC AGGATTTCTG TCATGGTCAG GTGATGTGGC CGCCGCTCAG CTCCATGCAG 
GTCCGGCTGG CTCCGGCTGG GAAATCCTGC AAGCAGGTGT GTCAAGAGGA GCAGCTCATC 
TGCGAGCCTT CATTCTTCCA GCATCTTAAT AAAGACAAGG ATCTGGCCAG GTACGGTGTG 
GAGTGTAAAA CGGTGGACTC AGCCAATGAT ATTGTAGTCC CGGCGTTCAG CGAATCGTCC 
CGGCACTGTG TGTTTCAGTC CGACCTGCTG CTGTTCAGCT GCGCTGGAGC TCATCAGACC 
CTCACGCGCA TCTGTCCCTG CAGAGACTAC ATGAAAGGCC AGGTGGCGCT GTGTAAAGAC 
TGCTTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001038776.1
CDS	282..2510
Translation	MGSVYLWKLSSQKLGFFLVSFGFIWGMMLLHFTIQQRATHESSAQLRMQILDLSKRYIKALAEENQSVMDGPYVGTMTAYDLKKTLAVLLDNIMQRLSKLESKVDNIMYNGTSANLTNGTGTPGPNPVNPEKVNVADLLNGAQEKCELPSLDGFPHCEGKIKWMKDMWRSDPCYGNYGVDGSTCSFFIYLSEVENWCPRLPWRIKNIGDDLDRKTQLEVRTSFDELYRVMSRREEFRWMMLRIKRMEEPWVSAVRSLANKQNLHHRKRKKILVHLGLLTKESGFKIAENAFSGGPLGELVQWSDLITTLYLLGHDIRISASLAELKEIMKKVMDNKSSCPTKGDKVVELIYIDIVGLAQFKKTLGPSWVHYQCMLRVLDSFGTEPEFNHAHYAQSKGHKTPWGKWNLNPQQFNTMFPHTPDNSFLGFVVEQHLNASDVKHIDDIKRQNQSLVYGKVDNFWKDKKKYLDIIHSYMEVHGTVHGTSTVHLPGYVKNHGILSGRDLQFLLRETKLFVGLSFPYEGPAPLEAIANGCAFLNPRFDPAKSSKNTDFFKGKPTLRELTSQHPYAEVYIGRPHVWTVNIEDPAEVENAIKAILSQKIEPYLPYEFTCEGMLQRVNAFIENQDFCHGQVMWPPLSSMQVRLAPAGKSCKQVCQEEQLICEPSFFQHLNKDKDLARYGVECKTVDSANDIVVPAFSESSRHCVFQSDLLLFSCAGAHQTLTRICPCRDYMKGQVALCKDCL

Target ORF information:

RefSeq Version	NM_001045311.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase (mgat5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001045311.1

ORF Insert Sequence:

ATGGGCTCCG TGTACTTGTG GAAGTTGTCT TCCCAAAAGT TGGGTTTCTT CCTCGTGAGT 
TTCGGCTTCA TTTGGGGGAT GATGCTGCTT CATTTCACCA TCCAGCAGAG GGCGACGCAT 
GAAAGCAGCG CGCAGCTACG AATGCAGATA CTGGACCTTA GCAAGCGATA CATTAAAGCC 
CTTGCAGAGG AGAACCAAAG CGTGATGGAT GGACCCTACG TAGGCACCAT GACAGCATAT 
GATCTGAAGA AGACTCTAGC TGTTCTTCTG GACAACATAA TGCAGAGATT GTCCAAGCTG 
GAGTCTAAAG TGGACAATAT CATGTACAAT GGCACCAGCG CCAACCTGAC CAACGGCACC 
GGGACTCCCG GACCAAACCC AGTCAACCCT GAGAAAGTCA ACGTGGCAGA TTTGCTAAAC 
GGAGCGCAGG AAAAATGTGA ACTGCCCTCG CTTGACGGGT TTCCACATTG TGAAGGCAAG 
ATAAAGTGGA TGAAGGACAT GTGGCGTTCG GACCCCTGCT ACGGCAACTA TGGTGTGGAT 
GGGTCCACCT GCTCATTCTT CATTTATTTG AGCGAGGTTG AAAACTGGTG CCCTCGTCTG 
CCGTGGAGGA TTAAGAACAT TGGGGATGAC TTGGACAGGA AAACACAGTT GGAGGTCCGC 
ACAAGTTTTG ACGAGTTGTA TCGCGTGATG TCGCGGCGTG AGGAGTTTCG CTGGATGATG 
CTGCGGATCA AGCGGATGGA GGAGCCGTGG GTCAGCGCCG TTCGCTCACT TGCCAACAAA 
CAAAACCTCC ACCACCGCAA ACGCAAGAAG ATCCTGGTTC ACCTGGGCTT GTTGACGAAG 
GAGTCTGGTT TTAAGATAGC GGAGAATGCG TTCAGCGGCG GTCCTCTGGG TGAGCTGGTG 
CAGTGGAGCG ACCTCATCAC CACACTCTAC CTGCTCGGAC ACGACATCCG AATTTCAGCC 
TCCCTCGCTG AGCTCAAAGA GATCATGAAG AAGGTGATGG ACAATAAATC CAGCTGCCCG 
ACCAAAGGCG ATAAAGTGGT GGAGCTGATT TACATCGACA TCGTGGGCCT GGCGCAGTTC 
AAGAAGACCC TCGGCCCGTC CTGGGTCCAT TATCAGTGTA TGCTGCGTGT GCTGGACTCA 
TTCGGGACGG AGCCGGAGTT CAATCACGCT CACTACGCCC AGTCCAAAGG CCACAAGACT 
CCGTGGGGCA AGTGGAACTT GAACCCGCAG CAGTTCAACA CCATGTTTCC TCACACTCCC 
GATAACAGTT TCCTGGGCTT CGTGGTTGAG CAGCATCTGA ACGCCAGTGA CGTCAAACAC 
ATCGACGACA TCAAGCGGCA AAACCAATCG CTGGTTTACG GAAAAGTGGA CAACTTCTGG 
AAGGATAAAA AGAAGTACCT AGACATTATT CACTCCTATA TGGAAGTTCA CGGCACAGTC 
CATGGCACTA GTACAGTCCA CCTGCCTGGC TACGTGAAGA ACCACGGCAT CCTGAGCGGA 
CGAGACCTGC AGTTCCTGCT GCGAGAGACC AAGCTTTTTG TTGGCCTGTC GTTTCCCTAT 
GAAGGCCCCG CCCCTCTCGA GGCGATAGCC AATGGCTGTG CATTCCTGAA CCCGAGGTTT 
GATCCTGCTA AGAGCAGCAA GAACACCGAT TTCTTCAAAG GCAAACCCAC ACTCAGAGAG 
TTGACGTCTC AGCATCCGTA TGCTGAGGTT TACATCGGCC GGCCGCACGT CTGGACCGTT 
AACATTGAAG ATCCGGCCGA GGTGGAGAAC GCCATTAAAG CCATACTGAG CCAGAAGATA 
GAGCCGTATC TGCCGTATGA GTTCACCTGT GAAGGGATGC TGCAGCGGGT CAATGCTTTC 
ATTGAGAACC AGGATTTCTG TCATGGTCAG GTGATGTGGC CGCCGCTCAG CTCCATGCAG 
GTCCGGCTGG CTCCGGCTGG GAAATCCTGC AAGCAGGTGT GTCAAGAGGA GCAGCTCATC 
TGCGAGCCTT CATTCTTCCA GCATCTTAAT AAAGACAAGG ATCTGGCCAG GTACGGTGTG 
GAGTGTAAAA CGGTGGACTC AGCCAATGAT ATTGTAGTCC CGGCGTTCAG CGAATCGTCC 
CGGCACTGTG TGTTTCAGTC CGACCTGCTG CTGTTCAGCT GCGCTGGAGC TCATCAGACC 
CTCACGCGCA TCTGTCCCTG CAGAGACTAC ATGAAAGGCC AGGTGGCGCT GTGTAAAGAC 
TGCTTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Loss of Mgat5a-mediated
Cell regeneration (London, England)53(2016)
Pei W,Huang SC,Xu L,Pettie K,Ceci ML,S?nchez M,Allende ML,Burgess SM

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

High-throughput gene targeting and phenotyping in zebrafish using CRISPR/Cas9.
Genome research25(7)1030-42(2015 Jul)
Varshney GK,Pei W,LaFave MC,Idol J,Xu L,Gallardo V,Carrington B,Bishop K,Jones M,Li M,Harper U,Huang SC,Prakash A,Chen W,Sood R,Ledin J,Burgess SM

In vivo protein trapping produces a functional expression codex of the vertebrate proteome.
Nature methods8(6)506-15(2011 Jun)
Clark KJ,Balciunas D,Pogoda HM,Ding Y,Westcot SE,Bedell VM,Greenwood TM,Urban MD,Skuster KJ,Petzold AM,Ni J,Nielsen AL,Patowary A,Scaria V,Sivasubbu S,Xu X,Hammerschmidt M,Ekker SC

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mgat5 cDNA ORF clone, Danio rerio(zebrafish)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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