crb2a cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	crb2a
Entrez Gene ID	723994
Full Name	crumbs family member 2a
Synonyms	crb2
General protein information	Preferred Names crumbs family member 2a Names protein crumbs homolog 2 crumbs homolog 2 crumbs homolog 2a oko meduzy ome
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001045299.1	NP_001038764.1	protein crumbs homolog 2

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	crb	NP_001247284.1
Caenorhabditis elegans (roundworm)	crb-1	NP_510822.1
Xenopus tropicalis (tropical clawed frog)	LOC100487355	XP_004918981.1
Gallus gallus (chicken)	CRB2	XP_004946067.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003873	XP_310433.5
Danio rerio (zebrafish)	crb2b	NP_001038627.1
Danio rerio (zebrafish)	crb2a	NP_001038764.1

Gene Ontology
Bibliography

Related articles in PubMed

Anisotropic M?ller glial scaffolding supports a multiplex lattice mosaic of photoreceptors in zebrafish retina.
Nagashima M, Hadidjojo J, Barthel LK, Lubensky DK, Raymond PA
Neural development12(1)20(2017 Nov)

Apico-basal Polarity Determinants Encoded by
Hazime K, Malicki JJ
Genetics207(3)1041-1051(2017 Nov)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

This study show that the undifferentiated CNS expresses Crb1, Crb2a, and Crb2b in distinct spatial and temporal patterns.
Title: Spatial-temporal expressions of Crumbs and Nagie oko and their interdependence in zebrafish central nervous system during early development.

It was demonstrated that the extracellular domains of Crb2a and Crb2b mediate a cell-cell adhesion function, which plays an essential role in maintaining the integrity of the photoreceptor layer.
Title: Crb apical polarity proteins maintain zebrafish retinal cone mosaics via intercellular binding of their extracellular domains.

Study shows that the FERM protein Mosaic Eyes (Moe) is a novel regulatory component of the Crumbs complex and coimmunoprecipitates with Ome/Crb2a and Nok (Pals1) from adult eye and in vitro interaction experiments suggest these interactions are direct.
Title: Mosaic Eyes is a novel component of the Crumbs complex and negatively regulates photoreceptor apical size.

oko meduzy (crb2a) locus encodes a crumbs gene homolog, essential for the proper apico-basal polarity of neural tube epithelia
Title: oko meduzy and related crumbs genes are determinants of apical cell features in the vertebrate embryo.

The following crb2a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the crb2a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa03357	NM_001045299.1 Latest version!	Danio rerio crumbs cell polarity complex component 2a (crb2a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

crb2a ( NM_001045299.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001038764.1 Danio rerio crumbs cell polarity complex component 2a (crb2a), mRNA.

Price & Availability↑

CloneID	ODa03357
Clone ID Related Accession (Same CDS sequence)	NM_001045299.1
Accession Version	NM_001045299.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4401bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-23
Organism	Danio rerio(zebrafish)
Product	protein crumbs homolog 2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from DQ314736.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DQ314736.1, BC162451.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168448 [ECO:0000350] ##Evidence-Data-END##

ATGGAGATCA GGAAAGTGCA TCTGAAGGGT CCCGGGACGG TTTTACTGAC CATGATGATG 
TTCAAATGGG GAATATTCTG TTCCGGATCA TCTGATTTGT GTGCATCTTC TCCATGTCAG 
AATGGGGCAA CATGCGTGGA TACAATGGAC GACTATGTGT GCTTGTGTTC GCGGGAAGGA 
GTGCGGTACA TGGGGAGGGA CTGTGAGGAG CTGTACAATG CTTGTATGTT TGCTGAATGT 
GAAGGGTGTG AGAGTGAACT TGGCACAGAC CACTACACCT GTCCAGAGGA CAAAGACGAG 
TGCAAAAGTG GCCCATGTGT TGGTCCCCAC TCTGAGTGTG TAAATGAGCA GAATGGCTAC 
AAATGCCTGT GCCCACCTGG TTTCGGGGGA GAAGACTGCA GTCACCGAAT CACAGACTGC 
ATTGATGAAC CCTGCCTCAA CAATGGCACC TGTAGACGGA TAGTGGATGG ATTTGAATGT 
GCCTGTTCTG ATGGATTTCG AGGCGAGACC TGTGAAGAAG ACCTAGATGA GTGTGATTCA 
CATCCCTGTC AGAATGGTGC AATCTGTTTG GATGGGGTCA ACAAGTATCA GTGCTTTTGT 
GTACCTGGAT ACCAAGGACA CAACTGTGAG ATTGACATCA ATGAGTGTGC ATCCCGACCT 
TGTTGGAACA ATGGGACCTG TATTAATGGG AAAGACAGAT ATCTGTGTGA TTGCCTACTG 
GGATATACAG GTGTAAACTG TGAGTTGGAA ATCGATGAAT GCGAGTCAAA TCCCTGTCAA 
AACGGAGCCA CCTGTCATGA CCTAGTGGGA CTGTACACCT GTGACTGTGT GCAGGGATTT 
GAAGGCTCGG ACTGTGAAAT TAATATTGAT GAATGTGAAA GTGGTCCTTG CCAGAATGGA 
GGACTTTGCC ACGACCGCAT TGATAGTTAT GAGTGTGAAT GTGAAGGCAC TGGATATATG 
GGAGACCACT GTGAAGAGGA TATTCCAGAA TGTGCATCAC ATCCTTGCCA ACATGGAGGA 
ACTTGTCTGG AGGGCATTAA CCACTACAAC TGTTCCTGTT GGCCAGGTTT TGAAGGTGGT 
AACTGTGAGG TGGATATAGA TGAATGTGCT GATGCTCCAT GTGAGAACGA TGGTGAATGT 
TTTGAAAAGT CAAATCCGGA ACACTGGGAA ACAGACTGGG AGTTCACTTA TGCCACAGCT 
GTGGGCTATG TATGTCAGTG CCAACCAGGA TACACAGGAG AGAATTGTTC AGTGAACATA 
AATGAATGTG TGTCCGAGCC ATGTCACAAT GGTGGAAGCT GTAAGGATTT GGTTAACGGA 
TACATGTGTG AATGCCCAGA GGGATTCACA GGTGTGGAAT GTGAGGTGAA TATAGATGAG 
TGTGAGAGTG CCCCCTGCCT TAATGGAGGT GTATGCGAAG ATGGAGTGGC TGAATACAAG 
TGTCACTGTG CAGAAGCTGA GGAAGGTCTG TTGCCATGGG GCGGACCCCA GTGCACGGTG 
CAGCTGCTTG GTTGCATTGG ACATGAATGC CAAAATGGTG CCACCTGCTT GCCATGGTTG 
GATGGAGAGA CACATGGACA TACCTGTCTT TGTCCACCTG GTTTCTATGA TGACGTATGC 
TCTATACAAA CCACATTCTC CTTTTCCTCA CCGAGGTTCT TTCTTATTGA GCTTCCATCA 
CTTGAACGCA GAAGGAGAGA GACAGAACCT GAACACCCCC TAGGGGTCCG CTTGCGATTC 
CGCACCACAC TGCCTGATAT GTTGCTGTTT TTTCGTGGTA ATGCGGAGTT CTTCCTATCC 
TTGGAGATTG TTGGGGGTGA GCTGCGTGCC AGGGCAACAT CAAAGGAGGA AGGATCACTG 
GAGGCACAAT TACCAGGACT TGTAAGTGAC GGAGACTGGC ATGAGGCAAT TGTGAACATT 
AATGAACATG ATATCAGGCT TGTCCTTCAA GTCAAAGGTG CAAACTGTAA TAATCAAGCA 
TGCAGAGTTG AAGACAAGCT ACAAGATGGT CATGAAACTT TCTTGCACCA TGACAGCCTC 
ACCAAAGTGT ATGTGGGAGG AGTACCAGAG GAGTATATTG GGCTTACCTT GAGCGGCCAA 
GGCTTTTTGG GTTGTATGGA GGATTTGCAG GTGGATGGTC ATCCTGTCCT GCCACATGAT 
CTGGGTCATG AGGAGGAGAG CGTTGAGTTG GGCTGTGTAA AGACAGAATG GTGCCAGGAA 
GATCCACAGC CCTGCTCTCA ACAAGGCCAT TGTGTTGATC TCTGGACGAG CTACCGCTGT 
GACTGTTACA GACCCCATTA TGGACAGGAC TGCAGCAAAG ATATGCCTAC CTGGACATTT 
GGTCATGAGA ACTCCACAAG TTTTCTTGCA TTTGAACTGC TGTCTGATTT TGGAAGTAAT 
TTTAGCGTGT CATTCTTCCT TCGATCCCTT AAATCAAGCG GATTATTATT TCAGCTACGT 
AGGTCAGGGG TTCAGGGGAC AGAAGATGAA CCTTATTTCA CCATATACCT GGAAATGGGA 
CGATTACAGG TGACCTCTAT GGCGGAGTCA CCCATTCTGT CATCACCAGT GTTTGTCTCC 
AATGGAGAAA AGCCACTGCT TCAGGTGGAC ATACAGGATG GTCAGGTTTT CTTTAATCAT 
GGAGGATATC GCTACGGGCT TGGCTTACTG CCCGACTTGG AAGTTCTGGA GGGAGACTTG 
TTGTATATTG GTGGAGTTCC AGGTGAAGAG GGTGCAACTG TTTGGGGTGG TCATTTTAAG 
GGCTGTCTGC AAGACCTGAG ACTGGACGAC GTGCATCTGG ATGTGGAAGA CTGGAATAAT 
ACACTCAGTG AGAGCATCTA TATCGCCAGT GATTCAGAAA ATGTCCTTCC TGGGTGCACG 
AGTGATGACA CTTGCAAGAT GGAGCCTTGC TTAAATGGAG GAGCATGCAC AGTCACATGG 
AATGACTTTA TTTGTTCCTG TCCAAAGGAT TTCACAGGAA AGACATGTGA AACCAGAGTT 
TGGTGTGTCA GCGATCCATG CATAAATGGT GGACACTGCG TAGATCTGTT GGATGGTTTT 
GAATGTATAG CGAACGCAAC CTTTGACAAT ACCCCTTTAC ACTACAGTGC TAAGGGATCT 
CTACTTGACC CAGTGACCAA TGTTACCATG AAGCTCCGTA CAAGACAGGA AAATGGTGTA 
CTACTTCGTG CATGGCGTGG GGAGGAGCTT CTACTGATTG GTCTGTTGGA TTCATCCATT 
CATGTGGAGA TTCACACAGC GAACAGTGTA GAACCAGTGA AGTTCTCTGG ACAGAGACGC 
ATAGCAGATG GAAACTGGCA TCGACTGCTG TTTGCGATGG CCCACCCAGA GCATGATGCG 
TCACAGTGGT TCGTTTTGGT GGATGGCATC ATCGATGGTA GCAGTGCCCC AGTGCTTGCA 
GGTAGTTTAA ATTTTCTTAA AGACATATCA AGCGAGGTTG CACTGGCCGA GACCTTCACA 
GGATGTCTAG GTGCTGTCAG AGTGGGTGGA GTTTACCTTC CATTTGTGGA CAATACCAAA 
TCACCCCAAA CAACACGGTT TTGGCGAAGA CCAGATGAGC ATGTACACTT GGGATGCTTT 
GGGGCACCAG TCTGCCGCTC TCATCCCTGT AGGCGTGGTG GCACATGTGT GGATCTCTTC 
AATAAATTTG GTTGTAAGTG CCCATCTGGC TGGGAAGGGA ACATTTGTGA GAAGGAAATA 
GACGAGTGTA TCTCGGGACC TTGCCTTCAT GGCAAGTGCA AGGACAAGCT TAATGGGTTT 
GACTGCTTAT GTCACCCAGG ATACGCAGGA CCTACATGTT CAGAAAACAT AGATGACTGC 
AAAGGATCGA GGTGTAAGAA TGGAGGAACC TGTGTTGATG GAGTTAATGA CTTTACCTGC 
ATCTGCCCAC CTAAGTACAG CGGAACACGT TGTCAATACA ACTACCCACC ACTAAAGTGT 
GCCCTGGATG TTGAATGTGA AAATGATGGT GTTTGCCATG ACACTCCGTG GGGGGCAAAC 
TGCACCTGTC CTCCAGGATT CACCGGAGAA AGATGTGAGA GAGAAATCGA TGAGTGTGCT 
TCTAGCCCTT GTCTAAATGG TGGATCGTGT CTGGATCGCC TCAACCGCTT TCAGTGTTTG 
TGTCCCGCAG GATTCAGTGG CCAGTTCTGT GAAACAAATA AACAAGCACA GCAAGATCAT 
ATACCATGGC TGGCGATAGC GGTTCCATTG GCTTGTGGCT GTATCCTCTT AGTGGCCATT 
GGTCTCATTT TCATGTTAAT GACTGCACGA AAGAAGCGGC AGTCGGAAGG AACTTATTCC 
CCAAGTCATC AGGAAGTTGC TGGTGCACGT CTGGAGATGG ACAGCATGCT GAAAGTACCA 
CCAGAAGAAC GGTTAATATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001038764.1
CDS	181..4581
Translation	MEIRKVHLKGPGTVLLTMMMFKWGIFCSGSSDLCASSPCQNGATCVDTMDDYVCLCSREGVRYMGRDCEELYNACMFAECEGCESELGTDHYTCPEDKDECKSGPCVGPHSECVNEQNGYKCLCPPGFGGEDCSHRITDCIDEPCLNNGTCRRIVDGFECACSDGFRGETCEEDLDECDSHPCQNGAICLDGVNKYQCFCVPGYQGHNCEIDINECASRPCWNNGTCINGKDRYLCDCLLGYTGVNCELEIDECESNPCQNGATCHDLVGLYTCDCVQGFEGSDCEINIDECESGPCQNGGLCHDRIDSYECECEGTGYMGDHCEEDIPECASHPCQHGGTCLEGINHYNCSCWPGFEGGNCEVDIDECADAPCENDGECFEKSNPEHWETDWEFTYATAVGYVCQCQPGYTGENCSVNINECVSEPCHNGGSCKDLVNGYMCECPEGFTGVECEVNIDECESAPCLNGGVCEDGVAEYKCHCAEAEEGLLPWGGPQCTVQLLGCIGHECQNGATCLPWLDGETHGHTCLCPPGFYDDVCSIQTTFSFSSPRFFLIELPSLERRRRETEPEHPLGVRLRFRTTLPDMLLFFRGNAEFFLSLEIVGGELRARATSKEEGSLEAQLPGLVSDGDWHEAIVNINEHDIRLVLQVKGANCNNQACRVEDKLQDGHETFLHHDSLTKVYVGGVPEEYIGLTLSGQGFLGCMEDLQVDGHPVLPHDLGHEEESVELGCVKTEWCQEDPQPCSQQGHCVDLWTSYRCDCYRPHYGQDCSKDMPTWTFGHENSTSFLAFELLSDFGSNFSVSFFLRSLKSSGLLFQLRRSGVQGTEDEPYFTIYLEMGRLQVTSMAESPILSSPVFVSNGEKPLLQVDIQDGQVFFNHGGYRYGLGLLPDLEVLEGDLLYIGGVPGEEGATVWGGHFKGCLQDLRLDDVHLDVEDWNNTLSESIYIASDSENVLPGCTSDDTCKMEPCLNGGACTVTWNDFICSCPKDFTGKTCETRVWCVSDPCINGGHCVDLLDGFECIANATFDNTPLHYSAKGSLLDPVTNVTMKLRTRQENGVLLRAWRGEELLLIGLLDSSIHVEIHTANSVEPVKFSGQRRIADGNWHRLLFAMAHPEHDASQWFVLVDGIIDGSSAPVLAGSLNFLKDISSEVALAETFTGCLGAVRVGGVYLPFVDNTKSPQTTRFWRRPDEHVHLGCFGAPVCRSHPCRRGGTCVDLFNKFGCKCPSGWEGNICEKEIDECISGPCLHGKCKDKLNGFDCLCHPGYAGPTCSENIDDCKGSRCKNGGTCVDGVNDFTCICPPKYSGTRCQYNYPPLKCALDVECENDGVCHDTPWGANCTCPPGFTGERCEREIDECASSPCLNGGSCLDRLNRFQCLCPAGFSGQFCETNKQAQQDHIPWLAIAVPLACGCILLVAIGLIFMLMTARKKRQSEGTYSPSHQEVAGARLEMDSMLKVPPEERLI

Target ORF information:

RefSeq Version	NM_001045299.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio crumbs cell polarity complex component 2a (crb2a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001045299.1

ORF Insert Sequence:

ATGGAGATCA GGAAAGTGCA TCTGAAGGGT CCCGGGACGG TTTTACTGAC CATGATGATG 
TTCAAATGGG GAATATTCTG TTCCGGATCA TCTGATTTGT GTGCATCTTC TCCATGTCAG 
AATGGGGCAA CATGCGTGGA TACAATGGAC GACTATGTGT GCTTGTGTTC GCGGGAAGGA 
GTGCGGTACA TGGGGAGGGA CTGTGAGGAG CTGTACAATG CTTGTATGTT TGCTGAATGT 
GAAGGGTGTG AGAGTGAACT TGGCACAGAC CACTACACCT GTCCAGAGGA CAAAGACGAG 
TGCAAAAGTG GCCCATGTGT TGGTCCCCAC TCTGAGTGTG TAAATGAGCA GAATGGCTAC 
AAATGCCTGT GCCCACCTGG TTTCGGGGGA GAAGACTGCA GTCACCGAAT CACAGACTGC 
ATTGATGAAC CCTGCCTCAA CAATGGCACC TGTAGACGGA TAGTGGATGG ATTTGAATGT 
GCCTGTTCTG ATGGATTTCG AGGCGAGACC TGTGAAGAAG ACCTAGATGA GTGTGATTCA 
CATCCCTGTC AGAATGGTGC AATCTGTTTG GATGGGGTCA ACAAGTATCA GTGCTTTTGT 
GTACCTGGAT ACCAAGGACA CAACTGTGAG ATTGACATCA ATGAGTGTGC ATCCCGACCT 
TGTTGGAACA ATGGGACCTG TATTAATGGG AAAGACAGAT ATCTGTGTGA TTGCCTACTG 
GGATATACAG GTGTAAACTG TGAGTTGGAA ATCGATGAAT GCGAGTCAAA TCCCTGTCAA 
AACGGAGCCA CCTGTCATGA CCTAGTGGGA CTGTACACCT GTGACTGTGT GCAGGGATTT 
GAAGGCTCGG ACTGTGAAAT TAATATTGAT GAATGTGAAA GTGGTCCTTG CCAGAATGGA 
GGACTTTGCC ACGACCGCAT TGATAGTTAT GAGTGTGAAT GTGAAGGCAC TGGATATATG 
GGAGACCACT GTGAAGAGGA TATTCCAGAA TGTGCATCAC ATCCTTGCCA ACATGGAGGA 
ACTTGTCTGG AGGGCATTAA CCACTACAAC TGTTCCTGTT GGCCAGGTTT TGAAGGTGGT 
AACTGTGAGG TGGATATAGA TGAATGTGCT GATGCTCCAT GTGAGAACGA TGGTGAATGT 
TTTGAAAAGT CAAATCCGGA ACACTGGGAA ACAGACTGGG AGTTCACTTA TGCCACAGCT 
GTGGGCTATG TATGTCAGTG CCAACCAGGA TACACAGGAG AGAATTGTTC AGTGAACATA 
AATGAATGTG TGTCCGAGCC ATGTCACAAT GGTGGAAGCT GTAAGGATTT GGTTAACGGA 
TACATGTGTG AATGCCCAGA GGGATTCACA GGTGTGGAAT GTGAGGTGAA TATAGATGAG 
TGTGAGAGTG CCCCCTGCCT TAATGGAGGT GTATGCGAAG ATGGAGTGGC TGAATACAAG 
TGTCACTGTG CAGAAGCTGA GGAAGGTCTG TTGCCATGGG GCGGACCCCA GTGCACGGTG 
CAGCTGCTTG GTTGCATTGG ACATGAATGC CAAAATGGTG CCACCTGCTT GCCATGGTTG 
GATGGAGAGA CACATGGACA TACCTGTCTT TGTCCACCTG GTTTCTATGA TGACGTATGC 
TCTATACAAA CCACATTCTC CTTTTCCTCA CCGAGGTTCT TTCTTATTGA GCTTCCATCA 
CTTGAACGCA GAAGGAGAGA GACAGAACCT GAACACCCCC TAGGGGTCCG CTTGCGATTC 
CGCACCACAC TGCCTGATAT GTTGCTGTTT TTTCGTGGTA ATGCGGAGTT CTTCCTATCC 
TTGGAGATTG TTGGGGGTGA GCTGCGTGCC AGGGCAACAT CAAAGGAGGA AGGATCACTG 
GAGGCACAAT TACCAGGACT TGTAAGTGAC GGAGACTGGC ATGAGGCAAT TGTGAACATT 
AATGAACATG ATATCAGGCT TGTCCTTCAA GTCAAAGGTG CAAACTGTAA TAATCAAGCA 
TGCAGAGTTG AAGACAAGCT ACAAGATGGT CATGAAACTT TCTTGCACCA TGACAGCCTC 
ACCAAAGTGT ATGTGGGAGG AGTACCAGAG GAGTATATTG GGCTTACCTT GAGCGGCCAA 
GGCTTTTTGG GTTGTATGGA GGATTTGCAG GTGGATGGTC ATCCTGTCCT GCCACATGAT 
CTGGGTCATG AGGAGGAGAG CGTTGAGTTG GGCTGTGTAA AGACAGAATG GTGCCAGGAA 
GATCCACAGC CCTGCTCTCA ACAAGGCCAT TGTGTTGATC TCTGGACGAG CTACCGCTGT 
GACTGTTACA GACCCCATTA TGGACAGGAC TGCAGCAAAG ATATGCCTAC CTGGACATTT 
GGTCATGAGA ACTCCACAAG TTTTCTTGCA TTTGAACTGC TGTCTGATTT TGGAAGTAAT 
TTTAGCGTGT CATTCTTCCT TCGATCCCTT AAATCAAGCG GATTATTATT TCAGCTACGT 
AGGTCAGGGG TTCAGGGGAC AGAAGATGAA CCTTATTTCA CCATATACCT GGAAATGGGA 
CGATTACAGG TGACCTCTAT GGCGGAGTCA CCCATTCTGT CATCACCAGT GTTTGTCTCC 
AATGGAGAAA AGCCACTGCT TCAGGTGGAC ATACAGGATG GTCAGGTTTT CTTTAATCAT 
GGAGGATATC GCTACGGGCT TGGCTTACTG CCCGACTTGG AAGTTCTGGA GGGAGACTTG 
TTGTATATTG GTGGAGTTCC AGGTGAAGAG GGTGCAACTG TTTGGGGTGG TCATTTTAAG 
GGCTGTCTGC AAGACCTGAG ACTGGACGAC GTGCATCTGG ATGTGGAAGA CTGGAATAAT 
ACACTCAGTG AGAGCATCTA TATCGCCAGT GATTCAGAAA ATGTCCTTCC TGGGTGCACG 
AGTGATGACA CTTGCAAGAT GGAGCCTTGC TTAAATGGAG GAGCATGCAC AGTCACATGG 
AATGACTTTA TTTGTTCCTG TCCAAAGGAT TTCACAGGAA AGACATGTGA AACCAGAGTT 
TGGTGTGTCA GCGATCCATG CATAAATGGT GGACACTGCG TAGATCTGTT GGATGGTTTT 
GAATGTATAG CGAACGCAAC CTTTGACAAT ACCCCTTTAC ACTACAGTGC TAAGGGATCT 
CTACTTGACC CAGTGACCAA TGTTACCATG AAGCTCCGTA CAAGACAGGA AAATGGTGTA 
CTACTTCGTG CATGGCGTGG GGAGGAGCTT CTACTGATTG GTCTGTTGGA TTCATCCATT 
CATGTGGAGA TTCACACAGC GAACAGTGTA GAACCAGTGA AGTTCTCTGG ACAGAGACGC 
ATAGCAGATG GAAACTGGCA TCGACTGCTG TTTGCGATGG CCCACCCAGA GCATGATGCG 
TCACAGTGGT TCGTTTTGGT GGATGGCATC ATCGATGGTA GCAGTGCCCC AGTGCTTGCA 
GGTAGTTTAA ATTTTCTTAA AGACATATCA AGCGAGGTTG CACTGGCCGA GACCTTCACA 
GGATGTCTAG GTGCTGTCAG AGTGGGTGGA GTTTACCTTC CATTTGTGGA CAATACCAAA 
TCACCCCAAA CAACACGGTT TTGGCGAAGA CCAGATGAGC ATGTACACTT GGGATGCTTT 
GGGGCACCAG TCTGCCGCTC TCATCCCTGT AGGCGTGGTG GCACATGTGT GGATCTCTTC 
AATAAATTTG GTTGTAAGTG CCCATCTGGC TGGGAAGGGA ACATTTGTGA GAAGGAAATA 
GACGAGTGTA TCTCGGGACC TTGCCTTCAT GGCAAGTGCA AGGACAAGCT TAATGGGTTT 
GACTGCTTAT GTCACCCAGG ATACGCAGGA CCTACATGTT CAGAAAACAT AGATGACTGC 
AAAGGATCGA GGTGTAAGAA TGGAGGAACC TGTGTTGATG GAGTTAATGA CTTTACCTGC 
ATCTGCCCAC CTAAGTACAG CGGAACACGT TGTCAATACA ACTACCCACC ACTAAAGTGT 
GCCCTGGATG TTGAATGTGA AAATGATGGT GTTTGCCATG ACACTCCGTG GGGGGCAAAC 
TGCACCTGTC CTCCAGGATT CACCGGAGAA AGATGTGAGA GAGAAATCGA TGAGTGTGCT 
TCTAGCCCTT GTCTAAATGG TGGATCGTGT CTGGATCGCC TCAACCGCTT TCAGTGTTTG 
TGTCCCGCAG GATTCAGTGG CCAGTTCTGT GAAACAAATA AACAAGCACA GCAAGATCAT 
ATACCATGGC TGGCGATAGC GGTTCCATTG GCTTGTGGCT GTATCCTCTT AGTGGCCATT 
GGTCTCATTT TCATGTTAAT GACTGCACGA AAGAAGCGGC AGTCGGAAGG AACTTATTCC 
CCAAGTCATC AGGAAGTTGC TGGTGCACGT CTGGAGATGG ACAGCATGCT GAAAGTACCA 
CCAGAAGAAC GGTTAATATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Anisotropic M?ller glial scaffolding supports a multiplex lattice mosaic of photoreceptors in zebrafish retina.
Neural development12(1)20(2017 Nov)
Nagashima M,Hadidjojo J,Barthel LK,Lubensky DK,Raymond PA

Apico-basal Polarity Determinants Encoded by
Genetics207(3)1041-1051(2017 Nov)
Hazime K,Malicki JJ

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

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crb2a cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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