nphs1 cDNA ORF clone, Danio rerio(zebrafish)

The following nphs1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the nphs1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa55129 XM_021481465.1
Latest version!
Danio rerio nephrosis 1, congenital, Finnish type (nephrin) (nphs1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
ODa55129 XM_017351014.2
Latest version!
Danio rerio nephrosis 1, congenital, Finnish type (nephrin) (nphs1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
ODa02033 NM_001040687.1
Latest version!
Danio rerio NPHS1 adhesion molecule, nephrin (nphs1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
View Old Accession Versions >>
ODa55129 XM_017351014.1 Danio rerio nephrosis 1, congenital, Finnish type (nephrin) (nphs1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa55129
    Clone ID Related Accession (Same CDS sequence) XM_017351014.1 , XM_021481465.1 , XM_017351014.2
    Accession Version XM_017351014.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3729bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product nephrin isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.6) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGATTCCT GGCTTTCTCT AGTCTTCTTG AGTGGCTGTT TTTTGCTTCT TGTGGATTTC 
    AAAGGTGTGT GTGGGCAGCA GGCGTTTAAG ACGGAGCCGC GTAATGTGAC GGTCAGAGCA
    GGGGCCACGG CTCTGCTGAA GTGTGAAGTG CTGAGGGCCT CAGGGGCCGT GCAGTGGGTT
    AAAGATGGGC TGCTCCTGGG ACCCCTGCGA TCTCTGCCTG GATACCCGCG ATACAGCATG
    ACAGGAGACC AGAAGAAAGG CCAGTACCAC CTTCAGATTC TAGACGTGAC ATTAGAAGAC
    GACGGCCCCT ACGAGTGCCA AGTGGGTCGG TCCGAGAGCA GTCGCCCCAT CGTGTCCCGT
    ACAGTCTGGC TCAATGTGCA GATCCCGTCT TCACAGCCGT ACTTCCAGCT GGAGTCTGAG
    GAGCCGTGGG TGGAAGGAGA GCAGTATACG GTGACCTGCA TCGCTCCTGA TGCTAAACCT
    CCTGCAGAAA TCACAATGTT TAGAGATGGA GAGGAGCTGC TGGAAAGCGA ATCGTATACA
    ATGTCAGGCT CGCAGGATAA GCTTCAGAAC ACTCATGCTG AAGTCACCAT CAGGGCACGA
    AGCTCGGACG ACACGCGACG CCTGACGTGT CGAGCGAAAA ACCCTGCTTC ACCTCGAGCT
    CTGGAGACTG GCATGACCAT GAAGGTTTAC TTTCCCCCAC AGGCTCCGAT GATCATGGGA
    TTGGAAAACG AAGAGGTCAA AGCCGGCTCC TTTCTCAAAG TGGTATGCAT GTCGTACGGG
    GGAAACCCTT TAGCCACTCT GCATTGGACT AAGAATGGAG AAGTCCTGTC CACAAGCTGG
    GATGTAGACT TTGTGTCCCG TCGAGCAAGC AGCGTGTTAA AAATGGAGGT GAAGCCGGAG
    GACAACCATG CTGTTTTGCA GTGCCAGAGC ATCAACCAAG TTACCCGTTC ACCAATGTCC
    CTAACCCGCA CTCTTACTGT CCTCTTTGAA CCAGCTGAGG TAACGTTACT GGGCTCCTTT
    GAAGCTGTGG AAGGAGAGGA GATCAATCTG AGCTGCTCCA CGTCCTCCAG TAATCCACCC
    GTTCACATCC GCTGGTGGCT CGGGTTCAAA GAGCTCAACA GAACAGAAGT TACCTTCGCC
    GAGGGGGATA ATGGTGGGAT GATGACGGTG TCTAACCTGA CGCATAAAGT GTCTCGTGAG
    GACAACGGTC TTTCTCTGCA ATGTGAGGCG TTCAACAAAG GCACGCGGTT CTCTAGCGTT
    CGGGCTGCAG AACTCATTGT TTACTATCCT CCTCAGAAGG TGTGGTTAGA TGCTCCTCCA
    CCAAATGTCT TTTTACACTC AGGTTCAACT CTTCGCTTAG TCTGTTTCTC CAGTGGTGGA
    AATCCTACAG GTCAACTTAC CTGGCTAAAG AACAATAAAG TCGTGCTGAC CGCATCGAAG
    CAGGTGTCTT CAGAGCGTGG TGTGTCACGA GAGCTGCTTC TCATCCTTCA TCCCAGTGAC
    AATTTGGCCA CCTACCGCTG TGACTCCTCC AACAAAGCCA AGAAAGTCCT GTCCACCCAA
    ACCAAATTCA GGGTTCTGTT TCCTGCAGTG AGTGTGAAGA TTGTGGCCAA GCAGAAGGAG
    CTGCGCAGAG GTCAGACTCT GGATCTGGAC TGTCTGGCCG GCAGCAGCAA TCCCACTGCA
    AATATCAGCT GGAGTCTGGG GCCCGTCATA CTGAAGGGAG TGGACCAGGC TCCGAAAAGG
    GCTGAATTTG GTGGGATGTC CCGGACCAGT AAGCTGTCCC TGACTCTTGA ATCCCATCAC
    TGGCGGAAGC GAATCACCTG TCAGGCCTTC AGTAATGTGC TTTCTGAGGG AGTCAACAAT
    TTCTACAGCC TCGATGTGCT CTTTCCTCCA GAGTTTTCAG ATGAGCAGCC TGATGAAGTC
    CAGGTCATTG AGGACGAAAC TGCCACGCTT CCGGCCAAAG TCTCTGCAAA CCCAGACGAA
    ATCACCTGCG AGTGGATCTT CCAGGGCGAG AAGCTTGTGA AAGAAAGAGA TCCTCGTTAT
    ACCTTCAGTG ACTGGACTCT TGAGATCGTG AACGTGTCTC GGCGGGACGG GGGCGATTAC
    ATCATCGAGT GCTCCAACGC GGAGGGGAGC AATCGCACCA AACTCAGACT GGATGTACAA
    TATTCCCCGA CTGTTCGTAT GAAGTCTGAC CCCGTGTTTG TGAACATAGG AGACACTGCA
    GACTTACTGT GTGTTGCTGA TGCCAATCCC ATCACCTCCA GTATGTTCTC ATGGAAGTGG
    ATGGGTGAAG GAGAAAGAGA TGAACTTGGA GAGGAAAGTG AAGATGACGG CACTGGCGTG
    TTGACTATAT ATGAGGTGAC TCGTGACCGT GCAGGTGTTT ACCAGTGTAC AGCAGACAAC
    GGCATTTCAC CTACTGGCAG TGTGGAAGGA CAGCTCATTG TACGCTTTGC TCCAGATCTT
    CAGAAGGGGC CACAGTGGAG GAAAGTGGCC AGTCGAGGGG ATGGCAGCAA CGATGCTAAT
    GTGGTGTGTC AGGCTCAAGG TGTTCCTCGT GTTCAATTTA GATGGGCCAA AAATGGGTTC
    CCGCTGGACC TCGGCAACCC CAGGTATGAG GAGACAACCG AGTCGACAGG CACCATCCAC
    ACCAGCATCC TAACAGTCAT AAATGTAAGC GCGGCTTTGG ACTATGCCAT ATTCACCTGC
    ACCGCTCACA ACAGCATGGG CGAAGATTCT CTGGACATCC AGCTCCTCAG CACCAACCAC
    CCTGATCCGC CATCAGACCT GAAGCTGCTC CGTGTCGACC AGACCTCCGT TACTCTGGAG
    TGGATGCCTG GATTTGACGG TGGTTTGACA CAGATCTTCC GTGTCAGGTA CCAGTGGGCA
    GGATCTGCAA GCTACATGTA TGTAGACGTG TTTCCTCCAA CAGCTACTGT ATATACAGTC
    ACAGGCCTCA ACCCTTCAAC TATGTACAAC TTCTCAGTCA ACGCTCTCAA TTCAATAGGA
    GAGAGCAGCT ATGCAGACAA CAACAAGATC CTGATGGTCA CCACTGCTGA TGCGAAGGAA
    GCTGACGGCG GGGTCTCCAC AGCGGACCAG GACTCGGCTC TTGCAGGTGG TGGCCTTCCT
    GTCTATGTGG TGGTGATCCT GGTTGTGGGC GGTGTTCTGT TCCTCCTGAA CCCATTCATC
    TGCTTTCTAT TGGCCAGATG GAAAAGAAGG CGGAGCCTGA AAGGGGACAC CAGCAGCATT
    TCACAGGAGA CCAAAAGTGA GGAGCAGAGG TCCACAGCGA GCAGCTCGAC CCGCTATGCG
    AGCAGAGAGC TTATCAACGC CTCGGCACAG CGAACGCTCC TCGTCGACAG CAGCTCAGAG
    CCTGACAGCA GCGTCTATGA GAGCTACGGA CGGCAGGAGA ATCCTCATTA TTACTACCCA
    ACTGAGGCCT ACAGTCCTGC CCTGTACGCT CACCCTGAAG GCCCTGAGGA TTACGATGGC
    AGACACGGCA CAAGCCCTTT CACTCATGTC TATGAGGAGG TGAGAGGAAT GTATGAGGAC
    CTGCAAGCCT GCTCACCGCT GCCCCCTGCT GCACACATTC ATCCACAAGC GCCGTTTTCC
    ACTTATTATG AGGGAAGAGC ATACAGTAAA GCTGATGTGG ACGTAACAAT TGGAGCTTAT
    GATTCAATGG TGTACAGACG CAGAAGAGAT ACAGACCTGC CGTTTGAGCT CAGAGGCGAG
    CTGGTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017206503.1
    CDS290..4018
    Translation

    Target ORF information:

    RefSeq Version XM_017351014.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio nephrosis 1, congenital, Finnish type (nephrin) (nphs1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017351014.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGATTCCT GGCTTTCTCT AGTCTTCTTG AGTGGCTGTT TTTTGCTTCT TGTGGATTTC 
    AAAGGTGTGT GTGGGCAGCA GGCGTTTAAG ACGGAGCCGC GTAATGTGAC GGTCAGAGCA
    GGGGCCACGG CTCTGCTGAA GTGTGAAGTG CTGAGGGCCT CAGGGGCCGT GCAGTGGGTT
    AAAGATGGGC TGCTCCTGGG ACCCCTGCGA TCTCTGCCTG GATACCCGCG ATACAGCATG
    ACAGGAGACC AGAAGAAAGG CCAGTACCAC CTTCAGATTC TAGACGTGAC ATTAGAAGAC
    GACGGCCCCT ACGAGTGCCA AGTGGGTCGG TCCGAGAGCA GTCGCCCCAT CGTGTCCCGT
    ACAGTCTGGC TCAATGTGCA GATCCCGTCT TCACAGCCGT ACTTCCAGCT GGAGTCTGAG
    GAGCCGTGGG TGGAAGGAGA GCAGTATACG GTGACCTGCA TCGCTCCTGA TGCTAAACCT
    CCTGCAGAAA TCACAATGTT TAGAGATGGA GAGGAGCTGC TGGAAAGCGA ATCGTATACA
    ATGTCAGGCT CGCAGGATAA GCTTCAGAAC ACTCATGCTG AAGTCACCAT CAGGGCACGA
    AGCTCGGACG ACACGCGACG CCTGACGTGT CGAGCGAAAA ACCCTGCTTC ACCTCGAGCT
    CTGGAGACTG GCATGACCAT GAAGGTTTAC TTTCCCCCAC AGGCTCCGAT GATCATGGGA
    TTGGAAAACG AAGAGGTCAA AGCCGGCTCC TTTCTCAAAG TGGTATGCAT GTCGTACGGG
    GGAAACCCTT TAGCCACTCT GCATTGGACT AAGAATGGAG AAGTCCTGTC CACAAGCTGG
    GATGTAGACT TTGTGTCCCG TCGAGCAAGC AGCGTGTTAA AAATGGAGGT GAAGCCGGAG
    GACAACCATG CTGTTTTGCA GTGCCAGAGC ATCAACCAAG TTACCCGTTC ACCAATGTCC
    CTAACCCGCA CTCTTACTGT CCTCTTTGAA CCAGCTGAGG TAACGTTACT GGGCTCCTTT
    GAAGCTGTGG AAGGAGAGGA GATCAATCTG AGCTGCTCCA CGTCCTCCAG TAATCCACCC
    GTTCACATCC GCTGGTGGCT CGGGTTCAAA GAGCTCAACA GAACAGAAGT TACCTTCGCC
    GAGGGGGATA ATGGTGGGAT GATGACGGTG TCTAACCTGA CGCATAAAGT GTCTCGTGAG
    GACAACGGTC TTTCTCTGCA ATGTGAGGCG TTCAACAAAG GCACGCGGTT CTCTAGCGTT
    CGGGCTGCAG AACTCATTGT TTACTATCCT CCTCAGAAGG TGTGGTTAGA TGCTCCTCCA
    CCAAATGTCT TTTTACACTC AGGTTCAACT CTTCGCTTAG TCTGTTTCTC CAGTGGTGGA
    AATCCTACAG GTCAACTTAC CTGGCTAAAG AACAATAAAG TCGTGCTGAC CGCATCGAAG
    CAGGTGTCTT CAGAGCGTGG TGTGTCACGA GAGCTGCTTC TCATCCTTCA TCCCAGTGAC
    AATTTGGCCA CCTACCGCTG TGACTCCTCC AACAAAGCCA AGAAAGTCCT GTCCACCCAA
    ACCAAATTCA GGGTTCTGTT TCCTGCAGTG AGTGTGAAGA TTGTGGCCAA GCAGAAGGAG
    CTGCGCAGAG GTCAGACTCT GGATCTGGAC TGTCTGGCCG GCAGCAGCAA TCCCACTGCA
    AATATCAGCT GGAGTCTGGG GCCCGTCATA CTGAAGGGAG TGGACCAGGC TCCGAAAAGG
    GCTGAATTTG GTGGGATGTC CCGGACCAGT AAGCTGTCCC TGACTCTTGA ATCCCATCAC
    TGGCGGAAGC GAATCACCTG TCAGGCCTTC AGTAATGTGC TTTCTGAGGG AGTCAACAAT
    TTCTACAGCC TCGATGTGCT CTTTCCTCCA GAGTTTTCAG ATGAGCAGCC TGATGAAGTC
    CAGGTCATTG AGGACGAAAC TGCCACGCTT CCGGCCAAAG TCTCTGCAAA CCCAGACGAA
    ATCACCTGCG AGTGGATCTT CCAGGGCGAG AAGCTTGTGA AAGAAAGAGA TCCTCGTTAT
    ACCTTCAGTG ACTGGACTCT TGAGATCGTG AACGTGTCTC GGCGGGACGG GGGCGATTAC
    ATCATCGAGT GCTCCAACGC GGAGGGGAGC AATCGCACCA AACTCAGACT GGATGTACAA
    TATTCCCCGA CTGTTCGTAT GAAGTCTGAC CCCGTGTTTG TGAACATAGG AGACACTGCA
    GACTTACTGT GTGTTGCTGA TGCCAATCCC ATCACCTCCA GTATGTTCTC ATGGAAGTGG
    ATGGGTGAAG GAGAAAGAGA TGAACTTGGA GAGGAAAGTG AAGATGACGG CACTGGCGTG
    TTGACTATAT ATGAGGTGAC TCGTGACCGT GCAGGTGTTT ACCAGTGTAC AGCAGACAAC
    GGCATTTCAC CTACTGGCAG TGTGGAAGGA CAGCTCATTG TACGCTTTGC TCCAGATCTT
    CAGAAGGGGC CACAGTGGAG GAAAGTGGCC AGTCGAGGGG ATGGCAGCAA CGATGCTAAT
    GTGGTGTGTC AGGCTCAAGG TGTTCCTCGT GTTCAATTTA GATGGGCCAA AAATGGGTTC
    CCGCTGGACC TCGGCAACCC CAGGTATGAG GAGACAACCG AGTCGACAGG CACCATCCAC
    ACCAGCATCC TAACAGTCAT AAATGTAAGC GCGGCTTTGG ACTATGCCAT ATTCACCTGC
    ACCGCTCACA ACAGCATGGG CGAAGATTCT CTGGACATCC AGCTCCTCAG CACCAACCAC
    CCTGATCCGC CATCAGACCT GAAGCTGCTC CGTGTCGACC AGACCTCCGT TACTCTGGAG
    TGGATGCCTG GATTTGACGG TGGTTTGACA CAGATCTTCC GTGTCAGGTA CCAGTGGGCA
    GGATCTGCAA GCTACATGTA TGTAGACGTG TTTCCTCCAA CAGCTACTGT ATATACAGTC
    ACAGGCCTCA ACCCTTCAAC TATGTACAAC TTCTCAGTCA ACGCTCTCAA TTCAATAGGA
    GAGAGCAGCT ATGCAGACAA CAACAAGATC CTGATGGTCA CCACTGCTGA TGCGAAGGAA
    GCTGACGGCG GGGTCTCCAC AGCGGACCAG GACTCGGCTC TTGCAGGTGG TGGCCTTCCT
    GTCTATGTGG TGGTGATCCT GGTTGTGGGC GGTGTTCTGT TCCTCCTGAA CCCATTCATC
    TGCTTTCTAT TGGCCAGATG GAAAAGAAGG CGGAGCCTGA AAGGGGACAC CAGCAGCATT
    TCACAGGAGA CCAAAAGTGA GGAGCAGAGG TCCACAGCGA GCAGCTCGAC CCGCTATGCG
    AGCAGAGAGC TTATCAACGC CTCGGCACAG CGAACGCTCC TCGTCGACAG CAGCTCAGAG
    CCTGACAGCA GCGTCTATGA GAGCTACGGA CGGCAGGAGA ATCCTCATTA TTACTACCCA
    ACTGAGGCCT ACAGTCCTGC CCTGTACGCT CACCCTGAAG GCCCTGAGGA TTACGATGGC
    AGACACGGCA CAAGCCCTTT CACTCATGTC TATGAGGAGG TGAGAGGAAT GTATGAGGAC
    CTGCAAGCCT GCTCACCGCT GCCCCCTGCT GCACACATTC ATCCACAAGC GCCGTTTTCC
    ACTTATTATG AGGGAAGAGC ATACAGTAAA GCTGATGTGG ACGTAACAAT TGGAGCTTAT
    GATTCAATGG TGTACAGACG CAGAAGAGAT ACAGACCTGC CGTTTGAGCT CAGAGGCGAG
    CTGGTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa55129
    Clone ID Related Accession (Same CDS sequence) XM_017351014.1 , XM_021481465.1 , XM_017351014.2
    Accession Version XM_021481465.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3729bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product nephrin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.7) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGATTCCT GGCTTTCTCT AGTCTTCTTG AGTGGCTGTT TTTTGCTTCT TGTGGATTTC 
    AAAGGTGTGT GTGGGCAGCA GGCGTTTAAG ACGGAGCCGC GTAATGTGAC GGTCAGAGCA
    GGGGCCACGG CTCTGCTGAA GTGTGAAGTG CTGAGGGCCT CAGGGGCCGT GCAGTGGGTT
    AAAGATGGGC TGCTCCTGGG ACCCCTGCGA TCTCTGCCTG GATACCCGCG ATACAGCATG
    ACAGGAGACC AGAAGAAAGG CCAGTACCAC CTTCAGATTC TAGACGTGAC ATTAGAAGAC
    GACGGCCCCT ACGAGTGCCA AGTGGGTCGG TCCGAGAGCA GTCGCCCCAT CGTGTCCCGT
    ACAGTCTGGC TCAATGTGCA GATCCCGTCT TCACAGCCGT ACTTCCAGCT GGAGTCTGAG
    GAGCCGTGGG TGGAAGGAGA GCAGTATACG GTGACCTGCA TCGCTCCTGA TGCTAAACCT
    CCTGCAGAAA TCACAATGTT TAGAGATGGA GAGGAGCTGC TGGAAAGCGA ATCGTATACA
    ATGTCAGGCT CGCAGGATAA GCTTCAGAAC ACTCATGCTG AAGTCACCAT CAGGGCACGA
    AGCTCGGACG ACACGCGACG CCTGACGTGT CGAGCGAAAA ACCCTGCTTC ACCTCGAGCT
    CTGGAGACTG GCATGACCAT GAAGGTTTAC TTTCCCCCAC AGGCTCCGAT GATCATGGGA
    TTGGAAAACG AAGAGGTCAA AGCCGGCTCC TTTCTCAAAG TGGTATGCAT GTCGTACGGG
    GGAAACCCTT TAGCCACTCT GCATTGGACT AAGAATGGAG AAGTCCTGTC CACAAGCTGG
    GATGTAGACT TTGTGTCCCG TCGAGCAAGC AGCGTGTTAA AAATGGAGGT GAAGCCGGAG
    GACAACCATG CTGTTTTGCA GTGCCAGAGC ATCAACCAAG TTACCCGTTC ACCAATGTCC
    CTAACCCGCA CTCTTACTGT CCTCTTTGAA CCAGCTGAGG TAACGTTACT GGGCTCCTTT
    GAAGCTGTGG AAGGAGAGGA GATCAATCTG AGCTGCTCCA CGTCCTCCAG TAATCCACCC
    GTTCACATCC GCTGGTGGCT CGGGTTCAAA GAGCTCAACA GAACAGAAGT TACCTTCGCC
    GAGGGGGATA ATGGTGGGAT GATGACGGTG TCTAACCTGA CGCATAAAGT GTCTCGTGAG
    GACAACGGTC TTTCTCTGCA ATGTGAGGCG TTCAACAAAG GCACGCGGTT CTCTAGCGTT
    CGGGCTGCAG AACTCATTGT TTACTATCCT CCTCAGAAGG TGTGGTTAGA TGCTCCTCCA
    CCAAATGTCT TTTTACACTC AGGTTCAACT CTTCGCTTAG TCTGTTTCTC CAGTGGTGGA
    AATCCTACAG GTCAACTTAC CTGGCTAAAG AACAATAAAG TCGTGCTGAC CGCATCGAAG
    CAGGTGTCTT CAGAGCGTGG TGTGTCACGA GAGCTGCTTC TCATCCTTCA TCCCAGTGAC
    AATTTGGCCA CCTACCGCTG TGACTCCTCC AACAAAGCCA AGAAAGTCCT GTCCACCCAA
    ACCAAATTCA GGGTTCTGTT TCCTGCAGTG AGTGTGAAGA TTGTGGCCAA GCAGAAGGAG
    CTGCGCAGAG GTCAGACTCT GGATCTGGAC TGTCTGGCCG GCAGCAGCAA TCCCACTGCA
    AATATCAGCT GGAGTCTGGG GCCCGTCATA CTGAAGGGAG TGGACCAGGC TCCGAAAAGG
    GCTGAATTTG GTGGGATGTC CCGGACCAGT AAGCTGTCCC TGACTCTTGA ATCCCATCAC
    TGGCGGAAGC GAATCACCTG TCAGGCCTTC AGTAATGTGC TTTCTGAGGG AGTCAACAAT
    TTCTACAGCC TCGATGTGCT CTTTCCTCCA GAGTTTTCAG ATGAGCAGCC TGATGAAGTC
    CAGGTCATTG AGGACGAAAC TGCCACGCTT CCGGCCAAAG TCTCTGCAAA CCCAGACGAA
    ATCACCTGCG AGTGGATCTT CCAGGGCGAG AAGCTTGTGA AAGAAAGAGA TCCTCGTTAT
    ACCTTCAGTG ACTGGACTCT TGAGATCGTG AACGTGTCTC GGCGGGACGG GGGCGATTAC
    ATCATCGAGT GCTCCAACGC GGAGGGGAGC AATCGCACCA AACTCAGACT GGATGTACAA
    TATTCCCCGA CTGTTCGTAT GAAGTCTGAC CCCGTGTTTG TGAACATAGG AGACACTGCA
    GACTTACTGT GTGTTGCTGA TGCCAATCCC ATCACCTCCA GTATGTTCTC ATGGAAGTGG
    ATGGGTGAAG GAGAAAGAGA TGAACTTGGA GAGGAAAGTG AAGATGACGG CACTGGCGTG
    TTGACTATAT ATGAGGTGAC TCGTGACCGT GCAGGTGTTT ACCAGTGTAC AGCAGACAAC
    GGCATTTCAC CTACTGGCAG TGTGGAAGGA CAGCTCATTG TACGCTTTGC TCCAGATCTT
    CAGAAGGGGC CACAGTGGAG GAAAGTGGCC AGTCGAGGGG ATGGCAGCAA CGATGCTAAT
    GTGGTGTGTC AGGCTCAAGG TGTTCCTCGT GTTCAATTTA GATGGGCCAA AAATGGGTTC
    CCGCTGGACC TCGGCAACCC CAGGTATGAG GAGACAACCG AGTCGACAGG CACCATCCAC
    ACCAGCATCC TAACAGTCAT AAATGTAAGC GCGGCTTTGG ACTATGCCAT ATTCACCTGC
    ACCGCTCACA ACAGCATGGG CGAAGATTCT CTGGACATCC AGCTCCTCAG CACCAACCAC
    CCTGATCCGC CATCAGACCT GAAGCTGCTC CGTGTCGACC AGACCTCCGT TACTCTGGAG
    TGGATGCCTG GATTTGACGG TGGTTTGACA CAGATCTTCC GTGTCAGGTA CCAGTGGGCA
    GGATCTGCAA GCTACATGTA TGTAGACGTG TTTCCTCCAA CAGCTACTGT ATATACAGTC
    ACAGGCCTCA ACCCTTCAAC TATGTACAAC TTCTCAGTCA ACGCTCTCAA TTCAATAGGA
    GAGAGCAGCT ATGCAGACAA CAACAAGATC CTGATGGTCA CCACTGCTGA TGCGAAGGAA
    GCTGACGGCG GGGTCTCCAC AGCGGACCAG GACTCGGCTC TTGCAGGTGG TGGCCTTCCT
    GTCTATGTGG TGGTGATCCT GGTTGTGGGC GGTGTTCTGT TCCTCCTGAA CCCATTCATC
    TGCTTTCTAT TGGCCAGATG GAAAAGAAGG CGGAGCCTGA AAGGGGACAC CAGCAGCATT
    TCACAGGAGA CCAAAAGTGA GGAGCAGAGG TCCACAGCGA GCAGCTCGAC CCGCTATGCG
    AGCAGAGAGC TTATCAACGC CTCGGCACAG CGAACGCTCC TCGTCGACAG CAGCTCAGAG
    CCTGACAGCA GCGTCTATGA GAGCTACGGA CGGCAGGAGA ATCCTCATTA TTACTACCCA
    ACTGAGGCCT ACAGTCCTGC CCTGTACGCT CACCCTGAAG GCCCTGAGGA TTACGATGGC
    AGACACGGCA CAAGCCCTTT CACTCATGTC TATGAGGAGG TGAGAGGAAT GTATGAGGAC
    CTGCAAGCCT GCTCACCGCT GCCCCCTGCT GCACACATTC ATCCACAAGC GCCGTTTTCC
    ACTTATTATG AGGGAAGAGC ATACAGTAAA GCTGATGTGG ACGTAACAAT TGGAGCTTAT
    GATTCAATGG TGTACAGACG CAGAAGAGAT ACAGACCTGC CGTTTGAGCT CAGAGGCGAG
    CTGGTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021337140.1
    CDS489..4217
    Translation

    Target ORF information:

    RefSeq Version XM_021481465.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio nephrosis 1, congenital, Finnish type (nephrin) (nphs1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021481465.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGATTCCT GGCTTTCTCT AGTCTTCTTG AGTGGCTGTT TTTTGCTTCT TGTGGATTTC 
    AAAGGTGTGT GTGGGCAGCA GGCGTTTAAG ACGGAGCCGC GTAATGTGAC GGTCAGAGCA
    GGGGCCACGG CTCTGCTGAA GTGTGAAGTG CTGAGGGCCT CAGGGGCCGT GCAGTGGGTT
    AAAGATGGGC TGCTCCTGGG ACCCCTGCGA TCTCTGCCTG GATACCCGCG ATACAGCATG
    ACAGGAGACC AGAAGAAAGG CCAGTACCAC CTTCAGATTC TAGACGTGAC ATTAGAAGAC
    GACGGCCCCT ACGAGTGCCA AGTGGGTCGG TCCGAGAGCA GTCGCCCCAT CGTGTCCCGT
    ACAGTCTGGC TCAATGTGCA GATCCCGTCT TCACAGCCGT ACTTCCAGCT GGAGTCTGAG
    GAGCCGTGGG TGGAAGGAGA GCAGTATACG GTGACCTGCA TCGCTCCTGA TGCTAAACCT
    CCTGCAGAAA TCACAATGTT TAGAGATGGA GAGGAGCTGC TGGAAAGCGA ATCGTATACA
    ATGTCAGGCT CGCAGGATAA GCTTCAGAAC ACTCATGCTG AAGTCACCAT CAGGGCACGA
    AGCTCGGACG ACACGCGACG CCTGACGTGT CGAGCGAAAA ACCCTGCTTC ACCTCGAGCT
    CTGGAGACTG GCATGACCAT GAAGGTTTAC TTTCCCCCAC AGGCTCCGAT GATCATGGGA
    TTGGAAAACG AAGAGGTCAA AGCCGGCTCC TTTCTCAAAG TGGTATGCAT GTCGTACGGG
    GGAAACCCTT TAGCCACTCT GCATTGGACT AAGAATGGAG AAGTCCTGTC CACAAGCTGG
    GATGTAGACT TTGTGTCCCG TCGAGCAAGC AGCGTGTTAA AAATGGAGGT GAAGCCGGAG
    GACAACCATG CTGTTTTGCA GTGCCAGAGC ATCAACCAAG TTACCCGTTC ACCAATGTCC
    CTAACCCGCA CTCTTACTGT CCTCTTTGAA CCAGCTGAGG TAACGTTACT GGGCTCCTTT
    GAAGCTGTGG AAGGAGAGGA GATCAATCTG AGCTGCTCCA CGTCCTCCAG TAATCCACCC
    GTTCACATCC GCTGGTGGCT CGGGTTCAAA GAGCTCAACA GAACAGAAGT TACCTTCGCC
    GAGGGGGATA ATGGTGGGAT GATGACGGTG TCTAACCTGA CGCATAAAGT GTCTCGTGAG
    GACAACGGTC TTTCTCTGCA ATGTGAGGCG TTCAACAAAG GCACGCGGTT CTCTAGCGTT
    CGGGCTGCAG AACTCATTGT TTACTATCCT CCTCAGAAGG TGTGGTTAGA TGCTCCTCCA
    CCAAATGTCT TTTTACACTC AGGTTCAACT CTTCGCTTAG TCTGTTTCTC CAGTGGTGGA
    AATCCTACAG GTCAACTTAC CTGGCTAAAG AACAATAAAG TCGTGCTGAC CGCATCGAAG
    CAGGTGTCTT CAGAGCGTGG TGTGTCACGA GAGCTGCTTC TCATCCTTCA TCCCAGTGAC
    AATTTGGCCA CCTACCGCTG TGACTCCTCC AACAAAGCCA AGAAAGTCCT GTCCACCCAA
    ACCAAATTCA GGGTTCTGTT TCCTGCAGTG AGTGTGAAGA TTGTGGCCAA GCAGAAGGAG
    CTGCGCAGAG GTCAGACTCT GGATCTGGAC TGTCTGGCCG GCAGCAGCAA TCCCACTGCA
    AATATCAGCT GGAGTCTGGG GCCCGTCATA CTGAAGGGAG TGGACCAGGC TCCGAAAAGG
    GCTGAATTTG GTGGGATGTC CCGGACCAGT AAGCTGTCCC TGACTCTTGA ATCCCATCAC
    TGGCGGAAGC GAATCACCTG TCAGGCCTTC AGTAATGTGC TTTCTGAGGG AGTCAACAAT
    TTCTACAGCC TCGATGTGCT CTTTCCTCCA GAGTTTTCAG ATGAGCAGCC TGATGAAGTC
    CAGGTCATTG AGGACGAAAC TGCCACGCTT CCGGCCAAAG TCTCTGCAAA CCCAGACGAA
    ATCACCTGCG AGTGGATCTT CCAGGGCGAG AAGCTTGTGA AAGAAAGAGA TCCTCGTTAT
    ACCTTCAGTG ACTGGACTCT TGAGATCGTG AACGTGTCTC GGCGGGACGG GGGCGATTAC
    ATCATCGAGT GCTCCAACGC GGAGGGGAGC AATCGCACCA AACTCAGACT GGATGTACAA
    TATTCCCCGA CTGTTCGTAT GAAGTCTGAC CCCGTGTTTG TGAACATAGG AGACACTGCA
    GACTTACTGT GTGTTGCTGA TGCCAATCCC ATCACCTCCA GTATGTTCTC ATGGAAGTGG
    ATGGGTGAAG GAGAAAGAGA TGAACTTGGA GAGGAAAGTG AAGATGACGG CACTGGCGTG
    TTGACTATAT ATGAGGTGAC TCGTGACCGT GCAGGTGTTT ACCAGTGTAC AGCAGACAAC
    GGCATTTCAC CTACTGGCAG TGTGGAAGGA CAGCTCATTG TACGCTTTGC TCCAGATCTT
    CAGAAGGGGC CACAGTGGAG GAAAGTGGCC AGTCGAGGGG ATGGCAGCAA CGATGCTAAT
    GTGGTGTGTC AGGCTCAAGG TGTTCCTCGT GTTCAATTTA GATGGGCCAA AAATGGGTTC
    CCGCTGGACC TCGGCAACCC CAGGTATGAG GAGACAACCG AGTCGACAGG CACCATCCAC
    ACCAGCATCC TAACAGTCAT AAATGTAAGC GCGGCTTTGG ACTATGCCAT ATTCACCTGC
    ACCGCTCACA ACAGCATGGG CGAAGATTCT CTGGACATCC AGCTCCTCAG CACCAACCAC
    CCTGATCCGC CATCAGACCT GAAGCTGCTC CGTGTCGACC AGACCTCCGT TACTCTGGAG
    TGGATGCCTG GATTTGACGG TGGTTTGACA CAGATCTTCC GTGTCAGGTA CCAGTGGGCA
    GGATCTGCAA GCTACATGTA TGTAGACGTG TTTCCTCCAA CAGCTACTGT ATATACAGTC
    ACAGGCCTCA ACCCTTCAAC TATGTACAAC TTCTCAGTCA ACGCTCTCAA TTCAATAGGA
    GAGAGCAGCT ATGCAGACAA CAACAAGATC CTGATGGTCA CCACTGCTGA TGCGAAGGAA
    GCTGACGGCG GGGTCTCCAC AGCGGACCAG GACTCGGCTC TTGCAGGTGG TGGCCTTCCT
    GTCTATGTGG TGGTGATCCT GGTTGTGGGC GGTGTTCTGT TCCTCCTGAA CCCATTCATC
    TGCTTTCTAT TGGCCAGATG GAAAAGAAGG CGGAGCCTGA AAGGGGACAC CAGCAGCATT
    TCACAGGAGA CCAAAAGTGA GGAGCAGAGG TCCACAGCGA GCAGCTCGAC CCGCTATGCG
    AGCAGAGAGC TTATCAACGC CTCGGCACAG CGAACGCTCC TCGTCGACAG CAGCTCAGAG
    CCTGACAGCA GCGTCTATGA GAGCTACGGA CGGCAGGAGA ATCCTCATTA TTACTACCCA
    ACTGAGGCCT ACAGTCCTGC CCTGTACGCT CACCCTGAAG GCCCTGAGGA TTACGATGGC
    AGACACGGCA CAAGCCCTTT CACTCATGTC TATGAGGAGG TGAGAGGAAT GTATGAGGAC
    CTGCAAGCCT GCTCACCGCT GCCCCCTGCT GCACACATTC ATCCACAAGC GCCGTTTTCC
    ACTTATTATG AGGGAAGAGC ATACAGTAAA GCTGATGTGG ACGTAACAAT TGGAGCTTAT
    GATTCAATGG TGTACAGACG CAGAAGAGAT ACAGACCTGC CGTTTGAGCT CAGAGGCGAG
    CTGGTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa55129
    Clone ID Related Accession (Same CDS sequence) XM_017351014.1 , XM_021481465.1 , XM_017351014.2
    Accession Version XM_017351014.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3729bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product nephrin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.7) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017351014.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGATTCCT GGCTTTCTCT AGTCTTCTTG AGTGGCTGTT TTTTGCTTCT TGTGGATTTC 
    AAAGGTGTGT GTGGGCAGCA GGCGTTTAAG ACGGAGCCGC GTAATGTGAC GGTCAGAGCA
    GGGGCCACGG CTCTGCTGAA GTGTGAAGTG CTGAGGGCCT CAGGGGCCGT GCAGTGGGTT
    AAAGATGGGC TGCTCCTGGG ACCCCTGCGA TCTCTGCCTG GATACCCGCG ATACAGCATG
    ACAGGAGACC AGAAGAAAGG CCAGTACCAC CTTCAGATTC TAGACGTGAC ATTAGAAGAC
    GACGGCCCCT ACGAGTGCCA AGTGGGTCGG TCCGAGAGCA GTCGCCCCAT CGTGTCCCGT
    ACAGTCTGGC TCAATGTGCA GATCCCGTCT TCACAGCCGT ACTTCCAGCT GGAGTCTGAG
    GAGCCGTGGG TGGAAGGAGA GCAGTATACG GTGACCTGCA TCGCTCCTGA TGCTAAACCT
    CCTGCAGAAA TCACAATGTT TAGAGATGGA GAGGAGCTGC TGGAAAGCGA ATCGTATACA
    ATGTCAGGCT CGCAGGATAA GCTTCAGAAC ACTCATGCTG AAGTCACCAT CAGGGCACGA
    AGCTCGGACG ACACGCGACG CCTGACGTGT CGAGCGAAAA ACCCTGCTTC ACCTCGAGCT
    CTGGAGACTG GCATGACCAT GAAGGTTTAC TTTCCCCCAC AGGCTCCGAT GATCATGGGA
    TTGGAAAACG AAGAGGTCAA AGCCGGCTCC TTTCTCAAAG TGGTATGCAT GTCGTACGGG
    GGAAACCCTT TAGCCACTCT GCATTGGACT AAGAATGGAG AAGTCCTGTC CACAAGCTGG
    GATGTAGACT TTGTGTCCCG TCGAGCAAGC AGCGTGTTAA AAATGGAGGT GAAGCCGGAG
    GACAACCATG CTGTTTTGCA GTGCCAGAGC ATCAACCAAG TTACCCGTTC ACCAATGTCC
    CTAACCCGCA CTCTTACTGT CCTCTTTGAA CCAGCTGAGG TAACGTTACT GGGCTCCTTT
    GAAGCTGTGG AAGGAGAGGA GATCAATCTG AGCTGCTCCA CGTCCTCCAG TAATCCACCC
    GTTCACATCC GCTGGTGGCT CGGGTTCAAA GAGCTCAACA GAACAGAAGT TACCTTCGCC
    GAGGGGGATA ATGGTGGGAT GATGACGGTG TCTAACCTGA CGCATAAAGT GTCTCGTGAG
    GACAACGGTC TTTCTCTGCA ATGTGAGGCG TTCAACAAAG GCACGCGGTT CTCTAGCGTT
    CGGGCTGCAG AACTCATTGT TTACTATCCT CCTCAGAAGG TGTGGTTAGA TGCTCCTCCA
    CCAAATGTCT TTTTACACTC AGGTTCAACT CTTCGCTTAG TCTGTTTCTC CAGTGGTGGA
    AATCCTACAG GTCAACTTAC CTGGCTAAAG AACAATAAAG TCGTGCTGAC CGCATCGAAG
    CAGGTGTCTT CAGAGCGTGG TGTGTCACGA GAGCTGCTTC TCATCCTTCA TCCCAGTGAC
    AATTTGGCCA CCTACCGCTG TGACTCCTCC AACAAAGCCA AGAAAGTCCT GTCCACCCAA
    ACCAAATTCA GGGTTCTGTT TCCTGCAGTG AGTGTGAAGA TTGTGGCCAA GCAGAAGGAG
    CTGCGCAGAG GTCAGACTCT GGATCTGGAC TGTCTGGCCG GCAGCAGCAA TCCCACTGCA
    AATATCAGCT GGAGTCTGGG GCCCGTCATA CTGAAGGGAG TGGACCAGGC TCCGAAAAGG
    GCTGAATTTG GTGGGATGTC CCGGACCAGT AAGCTGTCCC TGACTCTTGA ATCCCATCAC
    TGGCGGAAGC GAATCACCTG TCAGGCCTTC AGTAATGTGC TTTCTGAGGG AGTCAACAAT
    TTCTACAGCC TCGATGTGCT CTTTCCTCCA GAGTTTTCAG ATGAGCAGCC TGATGAAGTC
    CAGGTCATTG AGGACGAAAC TGCCACGCTT CCGGCCAAAG TCTCTGCAAA CCCAGACGAA
    ATCACCTGCG AGTGGATCTT CCAGGGCGAG AAGCTTGTGA AAGAAAGAGA TCCTCGTTAT
    ACCTTCAGTG ACTGGACTCT TGAGATCGTG AACGTGTCTC GGCGGGACGG GGGCGATTAC
    ATCATCGAGT GCTCCAACGC GGAGGGGAGC AATCGCACCA AACTCAGACT GGATGTACAA
    TATTCCCCGA CTGTTCGTAT GAAGTCTGAC CCCGTGTTTG TGAACATAGG AGACACTGCA
    GACTTACTGT GTGTTGCTGA TGCCAATCCC ATCACCTCCA GTATGTTCTC ATGGAAGTGG
    ATGGGTGAAG GAGAAAGAGA TGAACTTGGA GAGGAAAGTG AAGATGACGG CACTGGCGTG
    TTGACTATAT ATGAGGTGAC TCGTGACCGT GCAGGTGTTT ACCAGTGTAC AGCAGACAAC
    GGCATTTCAC CTACTGGCAG TGTGGAAGGA CAGCTCATTG TACGCTTTGC TCCAGATCTT
    CAGAAGGGGC CACAGTGGAG GAAAGTGGCC AGTCGAGGGG ATGGCAGCAA CGATGCTAAT
    GTGGTGTGTC AGGCTCAAGG TGTTCCTCGT GTTCAATTTA GATGGGCCAA AAATGGGTTC
    CCGCTGGACC TCGGCAACCC CAGGTATGAG GAGACAACCG AGTCGACAGG CACCATCCAC
    ACCAGCATCC TAACAGTCAT AAATGTAAGC GCGGCTTTGG ACTATGCCAT ATTCACCTGC
    ACCGCTCACA ACAGCATGGG CGAAGATTCT CTGGACATCC AGCTCCTCAG CACCAACCAC
    CCTGATCCGC CATCAGACCT GAAGCTGCTC CGTGTCGACC AGACCTCCGT TACTCTGGAG
    TGGATGCCTG GATTTGACGG TGGTTTGACA CAGATCTTCC GTGTCAGGTA CCAGTGGGCA
    GGATCTGCAA GCTACATGTA TGTAGACGTG TTTCCTCCAA CAGCTACTGT ATATACAGTC
    ACAGGCCTCA ACCCTTCAAC TATGTACAAC TTCTCAGTCA ACGCTCTCAA TTCAATAGGA
    GAGAGCAGCT ATGCAGACAA CAACAAGATC CTGATGGTCA CCACTGCTGA TGCGAAGGAA
    GCTGACGGCG GGGTCTCCAC AGCGGACCAG GACTCGGCTC TTGCAGGTGG TGGCCTTCCT
    GTCTATGTGG TGGTGATCCT GGTTGTGGGC GGTGTTCTGT TCCTCCTGAA CCCATTCATC
    TGCTTTCTAT TGGCCAGATG GAAAAGAAGG CGGAGCCTGA AAGGGGACAC CAGCAGCATT
    TCACAGGAGA CCAAAAGTGA GGAGCAGAGG TCCACAGCGA GCAGCTCGAC CCGCTATGCG
    AGCAGAGAGC TTATCAACGC CTCGGCACAG CGAACGCTCC TCGTCGACAG CAGCTCAGAG
    CCTGACAGCA GCGTCTATGA GAGCTACGGA CGGCAGGAGA ATCCTCATTA TTACTACCCA
    ACTGAGGCCT ACAGTCCTGC CCTGTACGCT CACCCTGAAG GCCCTGAGGA TTACGATGGC
    AGACACGGCA CAAGCCCTTT CACTCATGTC TATGAGGAGG TGAGAGGAAT GTATGAGGAC
    CTGCAAGCCT GCTCACCGCT GCCCCCTGCT GCACACATTC ATCCACAAGC GCCGTTTTCC
    ACTTATTATG AGGGAAGAGC ATACAGTAAA GCTGATGTGG ACGTAACAAT TGGAGCTTAT
    GATTCAATGG TGTACAGACG CAGAAGAGAT ACAGACCTGC CGTTTGAGCT CAGAGGCGAG
    CTGGTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017206503.1
    CDS605..4333
    Translation

    Target ORF information:

    RefSeq Version XM_017351014.2
    Organism Danio rerio(zebrafish)
    Definition Danio rerio nephrosis 1, congenital, Finnish type (nephrin) (nphs1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017351014.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGATTCCT GGCTTTCTCT AGTCTTCTTG AGTGGCTGTT TTTTGCTTCT TGTGGATTTC 
    AAAGGTGTGT GTGGGCAGCA GGCGTTTAAG ACGGAGCCGC GTAATGTGAC GGTCAGAGCA
    GGGGCCACGG CTCTGCTGAA GTGTGAAGTG CTGAGGGCCT CAGGGGCCGT GCAGTGGGTT
    AAAGATGGGC TGCTCCTGGG ACCCCTGCGA TCTCTGCCTG GATACCCGCG ATACAGCATG
    ACAGGAGACC AGAAGAAAGG CCAGTACCAC CTTCAGATTC TAGACGTGAC ATTAGAAGAC
    GACGGCCCCT ACGAGTGCCA AGTGGGTCGG TCCGAGAGCA GTCGCCCCAT CGTGTCCCGT
    ACAGTCTGGC TCAATGTGCA GATCCCGTCT TCACAGCCGT ACTTCCAGCT GGAGTCTGAG
    GAGCCGTGGG TGGAAGGAGA GCAGTATACG GTGACCTGCA TCGCTCCTGA TGCTAAACCT
    CCTGCAGAAA TCACAATGTT TAGAGATGGA GAGGAGCTGC TGGAAAGCGA ATCGTATACA
    ATGTCAGGCT CGCAGGATAA GCTTCAGAAC ACTCATGCTG AAGTCACCAT CAGGGCACGA
    AGCTCGGACG ACACGCGACG CCTGACGTGT CGAGCGAAAA ACCCTGCTTC ACCTCGAGCT
    CTGGAGACTG GCATGACCAT GAAGGTTTAC TTTCCCCCAC AGGCTCCGAT GATCATGGGA
    TTGGAAAACG AAGAGGTCAA AGCCGGCTCC TTTCTCAAAG TGGTATGCAT GTCGTACGGG
    GGAAACCCTT TAGCCACTCT GCATTGGACT AAGAATGGAG AAGTCCTGTC CACAAGCTGG
    GATGTAGACT TTGTGTCCCG TCGAGCAAGC AGCGTGTTAA AAATGGAGGT GAAGCCGGAG
    GACAACCATG CTGTTTTGCA GTGCCAGAGC ATCAACCAAG TTACCCGTTC ACCAATGTCC
    CTAACCCGCA CTCTTACTGT CCTCTTTGAA CCAGCTGAGG TAACGTTACT GGGCTCCTTT
    GAAGCTGTGG AAGGAGAGGA GATCAATCTG AGCTGCTCCA CGTCCTCCAG TAATCCACCC
    GTTCACATCC GCTGGTGGCT CGGGTTCAAA GAGCTCAACA GAACAGAAGT TACCTTCGCC
    GAGGGGGATA ATGGTGGGAT GATGACGGTG TCTAACCTGA CGCATAAAGT GTCTCGTGAG
    GACAACGGTC TTTCTCTGCA ATGTGAGGCG TTCAACAAAG GCACGCGGTT CTCTAGCGTT
    CGGGCTGCAG AACTCATTGT TTACTATCCT CCTCAGAAGG TGTGGTTAGA TGCTCCTCCA
    CCAAATGTCT TTTTACACTC AGGTTCAACT CTTCGCTTAG TCTGTTTCTC CAGTGGTGGA
    AATCCTACAG GTCAACTTAC CTGGCTAAAG AACAATAAAG TCGTGCTGAC CGCATCGAAG
    CAGGTGTCTT CAGAGCGTGG TGTGTCACGA GAGCTGCTTC TCATCCTTCA TCCCAGTGAC
    AATTTGGCCA CCTACCGCTG TGACTCCTCC AACAAAGCCA AGAAAGTCCT GTCCACCCAA
    ACCAAATTCA GGGTTCTGTT TCCTGCAGTG AGTGTGAAGA TTGTGGCCAA GCAGAAGGAG
    CTGCGCAGAG GTCAGACTCT GGATCTGGAC TGTCTGGCCG GCAGCAGCAA TCCCACTGCA
    AATATCAGCT GGAGTCTGGG GCCCGTCATA CTGAAGGGAG TGGACCAGGC TCCGAAAAGG
    GCTGAATTTG GTGGGATGTC CCGGACCAGT AAGCTGTCCC TGACTCTTGA ATCCCATCAC
    TGGCGGAAGC GAATCACCTG TCAGGCCTTC AGTAATGTGC TTTCTGAGGG AGTCAACAAT
    TTCTACAGCC TCGATGTGCT CTTTCCTCCA GAGTTTTCAG ATGAGCAGCC TGATGAAGTC
    CAGGTCATTG AGGACGAAAC TGCCACGCTT CCGGCCAAAG TCTCTGCAAA CCCAGACGAA
    ATCACCTGCG AGTGGATCTT CCAGGGCGAG AAGCTTGTGA AAGAAAGAGA TCCTCGTTAT
    ACCTTCAGTG ACTGGACTCT TGAGATCGTG AACGTGTCTC GGCGGGACGG GGGCGATTAC
    ATCATCGAGT GCTCCAACGC GGAGGGGAGC AATCGCACCA AACTCAGACT GGATGTACAA
    TATTCCCCGA CTGTTCGTAT GAAGTCTGAC CCCGTGTTTG TGAACATAGG AGACACTGCA
    GACTTACTGT GTGTTGCTGA TGCCAATCCC ATCACCTCCA GTATGTTCTC ATGGAAGTGG
    ATGGGTGAAG GAGAAAGAGA TGAACTTGGA GAGGAAAGTG AAGATGACGG CACTGGCGTG
    TTGACTATAT ATGAGGTGAC TCGTGACCGT GCAGGTGTTT ACCAGTGTAC AGCAGACAAC
    GGCATTTCAC CTACTGGCAG TGTGGAAGGA CAGCTCATTG TACGCTTTGC TCCAGATCTT
    CAGAAGGGGC CACAGTGGAG GAAAGTGGCC AGTCGAGGGG ATGGCAGCAA CGATGCTAAT
    GTGGTGTGTC AGGCTCAAGG TGTTCCTCGT GTTCAATTTA GATGGGCCAA AAATGGGTTC
    CCGCTGGACC TCGGCAACCC CAGGTATGAG GAGACAACCG AGTCGACAGG CACCATCCAC
    ACCAGCATCC TAACAGTCAT AAATGTAAGC GCGGCTTTGG ACTATGCCAT ATTCACCTGC
    ACCGCTCACA ACAGCATGGG CGAAGATTCT CTGGACATCC AGCTCCTCAG CACCAACCAC
    CCTGATCCGC CATCAGACCT GAAGCTGCTC CGTGTCGACC AGACCTCCGT TACTCTGGAG
    TGGATGCCTG GATTTGACGG TGGTTTGACA CAGATCTTCC GTGTCAGGTA CCAGTGGGCA
    GGATCTGCAA GCTACATGTA TGTAGACGTG TTTCCTCCAA CAGCTACTGT ATATACAGTC
    ACAGGCCTCA ACCCTTCAAC TATGTACAAC TTCTCAGTCA ACGCTCTCAA TTCAATAGGA
    GAGAGCAGCT ATGCAGACAA CAACAAGATC CTGATGGTCA CCACTGCTGA TGCGAAGGAA
    GCTGACGGCG GGGTCTCCAC AGCGGACCAG GACTCGGCTC TTGCAGGTGG TGGCCTTCCT
    GTCTATGTGG TGGTGATCCT GGTTGTGGGC GGTGTTCTGT TCCTCCTGAA CCCATTCATC
    TGCTTTCTAT TGGCCAGATG GAAAAGAAGG CGGAGCCTGA AAGGGGACAC CAGCAGCATT
    TCACAGGAGA CCAAAAGTGA GGAGCAGAGG TCCACAGCGA GCAGCTCGAC CCGCTATGCG
    AGCAGAGAGC TTATCAACGC CTCGGCACAG CGAACGCTCC TCGTCGACAG CAGCTCAGAG
    CCTGACAGCA GCGTCTATGA GAGCTACGGA CGGCAGGAGA ATCCTCATTA TTACTACCCA
    ACTGAGGCCT ACAGTCCTGC CCTGTACGCT CACCCTGAAG GCCCTGAGGA TTACGATGGC
    AGACACGGCA CAAGCCCTTT CACTCATGTC TATGAGGAGG TGAGAGGAAT GTATGAGGAC
    CTGCAAGCCT GCTCACCGCT GCCCCCTGCT GCACACATTC ATCCACAAGC GCCGTTTTCC
    ACTTATTATG AGGGAAGAGC ATACAGTAAA GCTGATGTGG ACGTAACAAT TGGAGCTTAT
    GATTCAATGG TGTACAGACG CAGAAGAGAT ACAGACCTGC CGTTTGAGCT CAGAGGCGAG
    CTGGTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa02033
    Clone ID Related Accession (Same CDS sequence) NM_001040687.1
    Accession Version NM_001040687.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3729bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-07
    Organism Danio rerio(zebrafish)
    Product nephrin
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from DQ466904.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DQ466904.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGATTCCT GGCTTTCTCT AGTCTTCTCG AGTGGCTGTT TTTTGCTTCT TGTGGATTTC 
    AAAGGTGTGT GTGGGCAGCA GGCGTTTAAG ACGGAGCCGC GTAATGTGAC GGTCAGAGCG
    GGGGCCACGG CTCTGCTGAA GTGTGAAGTG CTGAGGGCCT CAGGGGCCGT GCAGTGGGTT
    AAAGACGGGC TGCTCCTGAG ACCCCTGCGA TCTCTGCCTG GATACCCGCG ATACAGCATG
    ACAGGAGACC AGAAGAAAGG CCAGTACCAC CTTCAGATTC TAGACGTGAC ATTAGAAGAC
    GACGGCCCCT ACGAGTGCCA AGTGGGTCGG TCCGAGAGCA GTCGCCCCAT CGTGTCCCGT
    ACAGTCTGGC TCAATGTGCA GATCCCTTCT TCACAGCCGT ACTTCCAGCT GGAGTCTGAG
    GAGCCGTGGG TGGAAGGAGA GCAGTATACG GTGACCTGCA TCGCTCCTGA TGCTAAACCT
    CCTGCAGAAA TCACAATATT TAGAGATGGA GAGGAGCTGC TGGAAAGCGA ATCGTATACA
    ATGTCAGGCT CGCAGGATAA GCTTCAGAAC ACTCATGCTG AAGTCACCAT CAGGGCACGA
    AGCTCGGACG ACACGCGACG ACTGACGTGT CGAGCGAAAA ACCCTGCTTC ACCTCGAGCT
    CTGGAGACTG GCATGACCAT GAAGGTTTAC TTTCCCCCAC AGGCTCCGAT GATCATGGGA
    TTGGAAAACG AAGAGGTCAA AGCCGGCTCC TTTCTCAAAG TGGTATGCAT GTCGTACGGG
    GGAAACCCTT TAGCCACTCT GCATTGGACT AAGAATGGAG AAGTCCTGTC CACAAGCTGG
    GATGTAGACT TTGTGTCCCG TCGAGCCAGC AGCGTGTTAA AAATGGAGGT GAAGCCGGAG
    GACAACCATG CTGTTTTGCA GTGCCAGAGC ATCAACCAAG TTACCCGTTC ACCAATGTCC
    CTAACCCGCA CTCTTACTGT CCCCTTTGAA CCAGCTGAGG TAACGTTACT GGGCTCCTTT
    GAAGCTGTGG AAGGAGAGGA GATCAATCTG AGCTGCTCCA CGTCCTCCAG TAATCCACCC
    GTTCACATCC GCTGGTGGCT CGGGTTCAAA GAGCTCAACA GAACAGAAGT TACCTTCGCC
    GAGGGGGATA ATGGTGGGAT GATGACAGTG TCTAACCTGA CGCATAAAGT GTCTCGTGAG
    GACAACGGTC TTTCTCTGCA ATGTGAGGCG TTCAACAAAG GCACGCGGTT CTCTAGCGTT
    CGGGCTGCAG AACTCATTGT TTACTATCCT CCTCAGAAGG TGTGGTTAGA TGCTCCTCCA
    CCAAATGTCT TTTTACACTC AGGCTCAACT CTTCGCTTAG TCTGTTTCTC CAGTGGTGGA
    AATCCTACAG GTCAACTTAC CTGGCTAAAG AACAATAAAG TCGTGCTGAC CGCATCGAAG
    CAGGTGTCTT CAGAGCGTGG TGTGTCACGA GAGCTGCTTC TCACCCTCCA TCCCAGTGAC
    AATTTGGCCA CCTACCGCTG TGACTCCTCC AACAAAGCCA AGAAAGTCCT GTCCGCCCAA
    ACCAAATTCA GGGTTCTGTT TCCTGCAGTG AGTGTGAAGA TTGTGGCCAA GCAGAAGGAG
    CTGCGCAGAG GTCAGACTCT GGATCTGGAC TGTCTGGCCG GCAGCAGCAA TCCCACTGCA
    AATATCAGCT GGAGTCTGGG GCCCGTCATA CTGAAGGGAG TGGACCAGGC TCCGAAAAGG
    GCTGAATTTG GTGGGATGTC CCGGACCAGT AAGCTGTCCC TGACTCTTGA ACCCCATCAC
    TGGCGGAAGC GAATCACCTG TCAGGCCTTC AGTAATGTGC TTTCTGAGGG AGTCAACAAT
    TTCTACAGCC TCGATGTGCT CTTTCCTCCA GAGTTTTCAG CTGAGCAGCC TGATGAAGTC
    CAGGTCATTG AGGACGAAAC TGCCACGCTT CCGGCCAAAG TCTCTGCAAA CCCAGACGAA
    ATCACCTGCG AGTGGATCTT CCAGGGCGAG AAGCTTGTGA AAGAAAGAGA TCCTCGTTAT
    ACCTTCAGTG ACTGGACTCT TGAGATCGTG AACGTGTCTC GGCGGGACGG GGGCGATTAC
    ATCATCGAGT GCTCCAACGC GGAGGGGAGC AATCGCACCA AACTCAGACT GGATGTACAA
    TATTCCCCGA CTGTTCGTAT GAAGTCTGAC CCTGTGTTTG TGAACATAGG AGACACTGCA
    GACTTACTGT GTGTTGCTGA TGCCAATCCC ATCACCTCCA GTATGTTCTC ATGGAAGTGG
    ATGGGTGAAG GAGAAAGAGA TGAACTTGGA GAGGAAAGTG AAGATGACGG CACTGGCGTG
    TTGACTATAT ATGAGGTGAC TCGTGACCGT GCAGGTGTTT ACCAGTGTAC AGCAGACAAC
    GGCATTTCAC CTACTGGCAG TGTGGAAGGA CAGCTCATTG TACGCTTTGC TCCAGATCTT
    CAGAAGGGGC CACAGTGGAG GAAAGTGGCC AGTCGAGGGG ATGGCAGCAA CGATGCTAAT
    GTGGTGTGTC AGGCTCAAGG TGTTCCTCGT GTTCAATTTA GATGGGCCAA GAATGGGTTC
    CCGCTGGACC TCGGCAACCC CAGGTATGAG GAGACAACCG AGTCAACAGG CACCATCCAC
    ACCAGCATCC TAACAGTCAT AAATGTAAGC GCGGCCTTGG ACTATGCCAT ATTCACCTGC
    ACCGCTCACA ACAGCATGGG CGAAGATTCT CTGGACATCC AGCTCCTCAG CACCAACCAC
    CCTGATCCGC CATCAGACCT GAAGCTGCTC CGTGTCGACC AGACCTCCGT TACTCTGGAG
    TGGATGCCCG GATTTGACGG TGGTTTGACA CAGATCTTCC GTGTCAGGTA CCAGTGGGCA
    GGATCTGCAA GCTACATGTA TGTAGACGTG TTTCCTCCAA CAGCTACTGT ATATACAGTC
    ACAGGCCTTA ACCCTTCAAC TATGTACAAC TTCTCAGTCA ACGCTCTCAA TTCAATAGGG
    GAGAGCAGCT ATGCAGACAA CAACAAGATC CTGACAGTCA CCACTGCTGA TGCGAAGGAA
    GCTGACGGCG GGGTCTCCAC AGCGGACCAG GACTCAGCTC TTGCAGGTGG TGGCCTTCCT
    GTCTATGTGG TGGTGATCCT GGTTGTGGGC GGTGTTCTGT TCCTCCTGAA CCCATTCATC
    TGCTTTCTAT TGGCCAGATG GAAAAGAAGG CGGAGCCTGA AAGGGAACAC CAGCAGCATT
    TCACAAGAGA CCAAAAGTGA GGAGCAGAGG TCCACAGCGA GCAGCTCGAC CCGCTATGCG
    AGCAGAGAGC TTATCAACGC CTCGGCACAG CGAACGCTCC TCGTCGACAG CAGCTCGGAG
    CCTGACAGCA GCGTCTATGA GAGCTACGGA CGGCAGGAGA ATCCTCATTA TTACTACCCA
    ACTGAGGCCT ACAGTCCTGC CCTGTACGCT CACCCTGAAG GCCCTGAGGA TTGCGATGGC
    AGACACGGCA CAAGCCCTTT CACTCATGTC TATGAGGAGG TGAGAGGAAT GTATGAGGAC
    CTGCAGGCCT GCTCACCGCT GCCCCCTGCT GCACACATTC ATCCACAAGC GCCGTTTTCC
    ACTTATTATG AGGGAAGAGC ATACAGTAAA GCTGATGTGG ACGTAACAAT TGGAGCTTAT
    GATTCAATGG TGTACAGACG CAGAAGAGAT ACAGACCTGC CGTTTGAGCT CAGAGGCGAG
    CTGGTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001035777.1
    CDS13..3741
    Translation

    Target ORF information:

    RefSeq Version NM_001040687.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio NPHS1 adhesion molecule, nephrin (nphs1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001040687.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGATTCCT GGCTTTCTCT AGTCTTCTCG AGTGGCTGTT TTTTGCTTCT TGTGGATTTC 
    AAAGGTGTGT GTGGGCAGCA GGCGTTTAAG ACGGAGCCGC GTAATGTGAC GGTCAGAGCG
    GGGGCCACGG CTCTGCTGAA GTGTGAAGTG CTGAGGGCCT CAGGGGCCGT GCAGTGGGTT
    AAAGACGGGC TGCTCCTGAG ACCCCTGCGA TCTCTGCCTG GATACCCGCG ATACAGCATG
    ACAGGAGACC AGAAGAAAGG CCAGTACCAC CTTCAGATTC TAGACGTGAC ATTAGAAGAC
    GACGGCCCCT ACGAGTGCCA AGTGGGTCGG TCCGAGAGCA GTCGCCCCAT CGTGTCCCGT
    ACAGTCTGGC TCAATGTGCA GATCCCTTCT TCACAGCCGT ACTTCCAGCT GGAGTCTGAG
    GAGCCGTGGG TGGAAGGAGA GCAGTATACG GTGACCTGCA TCGCTCCTGA TGCTAAACCT
    CCTGCAGAAA TCACAATATT TAGAGATGGA GAGGAGCTGC TGGAAAGCGA ATCGTATACA
    ATGTCAGGCT CGCAGGATAA GCTTCAGAAC ACTCATGCTG AAGTCACCAT CAGGGCACGA
    AGCTCGGACG ACACGCGACG ACTGACGTGT CGAGCGAAAA ACCCTGCTTC ACCTCGAGCT
    CTGGAGACTG GCATGACCAT GAAGGTTTAC TTTCCCCCAC AGGCTCCGAT GATCATGGGA
    TTGGAAAACG AAGAGGTCAA AGCCGGCTCC TTTCTCAAAG TGGTATGCAT GTCGTACGGG
    GGAAACCCTT TAGCCACTCT GCATTGGACT AAGAATGGAG AAGTCCTGTC CACAAGCTGG
    GATGTAGACT TTGTGTCCCG TCGAGCCAGC AGCGTGTTAA AAATGGAGGT GAAGCCGGAG
    GACAACCATG CTGTTTTGCA GTGCCAGAGC ATCAACCAAG TTACCCGTTC ACCAATGTCC
    CTAACCCGCA CTCTTACTGT CCCCTTTGAA CCAGCTGAGG TAACGTTACT GGGCTCCTTT
    GAAGCTGTGG AAGGAGAGGA GATCAATCTG AGCTGCTCCA CGTCCTCCAG TAATCCACCC
    GTTCACATCC GCTGGTGGCT CGGGTTCAAA GAGCTCAACA GAACAGAAGT TACCTTCGCC
    GAGGGGGATA ATGGTGGGAT GATGACAGTG TCTAACCTGA CGCATAAAGT GTCTCGTGAG
    GACAACGGTC TTTCTCTGCA ATGTGAGGCG TTCAACAAAG GCACGCGGTT CTCTAGCGTT
    CGGGCTGCAG AACTCATTGT TTACTATCCT CCTCAGAAGG TGTGGTTAGA TGCTCCTCCA
    CCAAATGTCT TTTTACACTC AGGCTCAACT CTTCGCTTAG TCTGTTTCTC CAGTGGTGGA
    AATCCTACAG GTCAACTTAC CTGGCTAAAG AACAATAAAG TCGTGCTGAC CGCATCGAAG
    CAGGTGTCTT CAGAGCGTGG TGTGTCACGA GAGCTGCTTC TCACCCTCCA TCCCAGTGAC
    AATTTGGCCA CCTACCGCTG TGACTCCTCC AACAAAGCCA AGAAAGTCCT GTCCGCCCAA
    ACCAAATTCA GGGTTCTGTT TCCTGCAGTG AGTGTGAAGA TTGTGGCCAA GCAGAAGGAG
    CTGCGCAGAG GTCAGACTCT GGATCTGGAC TGTCTGGCCG GCAGCAGCAA TCCCACTGCA
    AATATCAGCT GGAGTCTGGG GCCCGTCATA CTGAAGGGAG TGGACCAGGC TCCGAAAAGG
    GCTGAATTTG GTGGGATGTC CCGGACCAGT AAGCTGTCCC TGACTCTTGA ACCCCATCAC
    TGGCGGAAGC GAATCACCTG TCAGGCCTTC AGTAATGTGC TTTCTGAGGG AGTCAACAAT
    TTCTACAGCC TCGATGTGCT CTTTCCTCCA GAGTTTTCAG CTGAGCAGCC TGATGAAGTC
    CAGGTCATTG AGGACGAAAC TGCCACGCTT CCGGCCAAAG TCTCTGCAAA CCCAGACGAA
    ATCACCTGCG AGTGGATCTT CCAGGGCGAG AAGCTTGTGA AAGAAAGAGA TCCTCGTTAT
    ACCTTCAGTG ACTGGACTCT TGAGATCGTG AACGTGTCTC GGCGGGACGG GGGCGATTAC
    ATCATCGAGT GCTCCAACGC GGAGGGGAGC AATCGCACCA AACTCAGACT GGATGTACAA
    TATTCCCCGA CTGTTCGTAT GAAGTCTGAC CCTGTGTTTG TGAACATAGG AGACACTGCA
    GACTTACTGT GTGTTGCTGA TGCCAATCCC ATCACCTCCA GTATGTTCTC ATGGAAGTGG
    ATGGGTGAAG GAGAAAGAGA TGAACTTGGA GAGGAAAGTG AAGATGACGG CACTGGCGTG
    TTGACTATAT ATGAGGTGAC TCGTGACCGT GCAGGTGTTT ACCAGTGTAC AGCAGACAAC
    GGCATTTCAC CTACTGGCAG TGTGGAAGGA CAGCTCATTG TACGCTTTGC TCCAGATCTT
    CAGAAGGGGC CACAGTGGAG GAAAGTGGCC AGTCGAGGGG ATGGCAGCAA CGATGCTAAT
    GTGGTGTGTC AGGCTCAAGG TGTTCCTCGT GTTCAATTTA GATGGGCCAA GAATGGGTTC
    CCGCTGGACC TCGGCAACCC CAGGTATGAG GAGACAACCG AGTCAACAGG CACCATCCAC
    ACCAGCATCC TAACAGTCAT AAATGTAAGC GCGGCCTTGG ACTATGCCAT ATTCACCTGC
    ACCGCTCACA ACAGCATGGG CGAAGATTCT CTGGACATCC AGCTCCTCAG CACCAACCAC
    CCTGATCCGC CATCAGACCT GAAGCTGCTC CGTGTCGACC AGACCTCCGT TACTCTGGAG
    TGGATGCCCG GATTTGACGG TGGTTTGACA CAGATCTTCC GTGTCAGGTA CCAGTGGGCA
    GGATCTGCAA GCTACATGTA TGTAGACGTG TTTCCTCCAA CAGCTACTGT ATATACAGTC
    ACAGGCCTTA ACCCTTCAAC TATGTACAAC TTCTCAGTCA ACGCTCTCAA TTCAATAGGG
    GAGAGCAGCT ATGCAGACAA CAACAAGATC CTGACAGTCA CCACTGCTGA TGCGAAGGAA
    GCTGACGGCG GGGTCTCCAC AGCGGACCAG GACTCAGCTC TTGCAGGTGG TGGCCTTCCT
    GTCTATGTGG TGGTGATCCT GGTTGTGGGC GGTGTTCTGT TCCTCCTGAA CCCATTCATC
    TGCTTTCTAT TGGCCAGATG GAAAAGAAGG CGGAGCCTGA AAGGGAACAC CAGCAGCATT
    TCACAAGAGA CCAAAAGTGA GGAGCAGAGG TCCACAGCGA GCAGCTCGAC CCGCTATGCG
    AGCAGAGAGC TTATCAACGC CTCGGCACAG CGAACGCTCC TCGTCGACAG CAGCTCGGAG
    CCTGACAGCA GCGTCTATGA GAGCTACGGA CGGCAGGAGA ATCCTCATTA TTACTACCCA
    ACTGAGGCCT ACAGTCCTGC CCTGTACGCT CACCCTGAAG GCCCTGAGGA TTGCGATGGC
    AGACACGGCA CAAGCCCTTT CACTCATGTC TATGAGGAGG TGAGAGGAAT GTATGAGGAC
    CTGCAGGCCT GCTCACCGCT GCCCCCTGCT GCACACATTC ATCCACAAGC GCCGTTTTCC
    ACTTATTATG AGGGAAGAGC ATACAGTAAA GCTGATGTGG ACGTAACAAT TGGAGCTTAT
    GATTCAATGG TGTACAGACG CAGAAGAGAT ACAGACCTGC CGTTTGAGCT CAGAGGCGAG
    CTGGTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.