tbk1 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	tbk1
Entrez Gene ID	692289
Full Name	TANK-binding kinase 1
Synonyms	wu:fk70c05,zgc:136548
General protein information	Preferred Names TANK-binding kinase 1 Names serine/threonine-protein kinase TBK1 TANK-binding kinase 1 transcript variant X1 TANK-binding kinase 1 transcript variant X2
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001044748.2	NP_001038213.2	serine/threonine-protein kinase TBK1
XM_021475140.1	XP_021330815.1	serine/threonine-protein kinase TBK1 isoform X1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	TBK1	XP_538266.3
Mus musculus (house mouse)	Tbk1	NP_062760.3
Drosophila melanogaster (fruit fly)	ik2	NP_610048.2
Bos taurus (cattle)	TBK1	NP_001179684.1
Rattus norvegicus (Norway rat)	Tbk1	NP_001100256.1
Xenopus tropicalis (tropical clawed frog)	tbk1	NP_001135652.1
Pan troglodytes (chimpanzee)	TBK1	XP_509194.2
	TBK1	XP_001116924.1
Gallus gallus (chicken)	TBK1	NP_001186487.1
Danio rerio (zebrafish)	tbk1	NP_001038213.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009565	XP_318573.3
Homo sapiens (human)	TBK1	NP_037386.1

Gene Ontology
Bibliography

Related articles in PubMed

Evolution of complexity in the zebrafish synapse proteome.
Bay?s ?, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 Mar)

Zebra Fish Lacking Adaptive Immunity Acquire an Antiviral Alert State Characterized by Upregulated Gene Expression of Apoptosis, Multigene Families, and Interferon-Related Genes.
Garc?a-Valtanen P, Mart?nez-L?pez A, L?pez-Mu?oz A, Bello-Perez M, Medina-Gali RM, Ortega-Villaiz?n MD, Varela M, Figueras A, Mulero V, Novoa B, Estepa A, Coll J
Frontiers in immunology8121(2017)

FTR83, a Member of the Large Fish-Specific finTRIM Family, Triggers IFN Pathway and Counters Viral Infection.
Langevin C, Aleksejeva E, Houel A, Briolat V, Torhy C, Lunazzi A, Levraud JP, Boudinot P
Frontiers in immunology8617(2017)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

this study shows that STAT6 negatively regulates IFNphi1 production by attenuating the kinase activity of TANK-binding kinase 1
Title: Zebrafish STAT6 negatively regulates IFN?1 production by attenuating the kinase activity of TANK-binding kinase 1.

Fish IRF6 is distinguished from the homolog of mammals by being a positive regulator of IFN transcription and phosphorylated by MyD88 and TBK1, suggesting that differences in the IRF6 regulation pattern exist between lower and higher vertebrates.
Title: Fish IRF6 is a positive regulator of IFN expression and involved in both of the MyD88 and TBK1 pathways.

The following tbk1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the tbk1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa31635	NM_001044748.2 Latest version!	Danio rerio TANK-binding kinase 1 (tbk1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
ODa31635	XM_021475140.1 Latest version!	Danio rerio TANK-binding kinase 1 (tbk1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

tbk1 ( NM_001044748.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001038213.2 Danio rerio TANK-binding kinase 1 (tbk1), mRNA.

Price & Availability↑

CloneID	ODa31635
Clone ID Related Accession (Same CDS sequence)	NM_001044748.2 , XM_021475140.1
Accession Version	NM_001044748.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2184bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-09
Organism	Danio rerio(zebrafish)
Product	serine/threonine-protein kinase TBK1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from FP245483.5, DQ860098.1 and FP017216.4. On Jul 16, 2011 this sequence version replaced NM_001044748.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: GDQQ01021384.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCAGAGTA CGGCCAATTA CCTGTGGATG ATGTCCGACC TGCTGGGTCA GGGAGCCACA 
GCTAACGTGT ACCGCGGTCG ACACAAGAAA ACAGGCGATC TGTACGCCGT CAAAGTGTTC 
AACAACCTGA GTTTCCTGCG GCCGCTGGAC GTGCAGATGA GGGAGTTTGA GGTGCTGAAG 
AAACTCAATC ACAAGAACAT CGTCAAGCTG TTCGCCGTCG AGGAGGAGTC GAACACACGT 
CATAAGGTGC TGGTGATGGA GTACTGCCCC TGCGGGAGTC TCTACACCGT CCTGGAGGAG 
CCCACCAATG CGTACGGCCT GCCGGAGGAC GAGTTCCTCA TCGTTCTGCA GGACGTCGTG 
GCGGGAATGA ATCACCTGCG CGAGTACGGC ATCGTGCATC GAGACATCAA GCCAGGGAAC 
ATCATGCGAG TGATTGGAGA CGACGGATTC TCTGTGTACA AACTCACCGA CTTCGGAGCC 
GCTCGCGAAC TGGAGGACGA CGAGCAGTTC GTATCGCTGT ACGGCACAGA GGAGTATCTG 
CATCCAGACA TGTACGAGCG GGCGGTGCTG AGGAAAGACC ACCAGAAGAA ATACGGCGCT 
ACGGTGGATC TGTGGAGCAT CGGAGTCACG TTTTACCACG CCGCCACCGG CAGCCTGCCG 
TTCAGACCCT TCGAAGGGCC GCGCAGAAAC AAAGAAGTCA TGTATAAGAT CATCACAGAG 
AAGCCCCCCG GCGCCATCTC CGGACACCAG AAGTTTGAAA ATGGGAAGAT CGAGTGGAGC 
TCCGAGATGC CCATCTCCTG CAGCCTGTCC AAGGGTCTGC AGAGCCTCCT GACGCCGGTG 
CTGGCGAATA TCCTGGAAGC GGATCAGGAG AAGTGCTGGG GGTTTGATCA GTTCTTCGCA 
GAGACCAGCG ACATCTTACA CCGCATCGTG GTCTACGTCT TCAGTCTGCA GCAGGCCACG 
CTGCACCACG TCTACATACA CACTTACAAC ACAGCGAACC TCTTCCAGGA GCTGCTGTTC 
AGACGCACCA ACATCACTCC GTCCCATCAG GAGCTGCTCT ACGAGGGCCG ACGGCTCGTC 
CTCGACCCCA ACCGGCAAGC ACAAACCTTT CCCAAGACCT CCAGAGACAA TCCCATCATG 
CTCCTGTGCA GAGACCCCGT CAACACCGTC GGCCTGCTGT TTGAGGACCC CAGCCCACCC 
AAAGTACAGC CGCGCTACGA CCTGGACCTG GACGCCAGCT ACGCCAAGAC GTTCGCAGGT 
GATGTGGGAT ACCTGTGGAA AACATCAGAT TCTCTGCTTC TGTACCAGGA GCTGGTGAGG 
AAAGGAGTCC GAGGCCTGAA TGAGCTGATC AGAGATGAGT ACAGTGAGAC GATGCACAAG 
AAGACGGAGG TGTTCCACCT GTGCAGCCAC TGCAGCCAGA CACTGGAGCG CAGCGAACAA 
CTCTGTGAGG CGCTGATGCA GGGAAATATC CTGTCAGCCG AGTATGACGA GATCCGAGAC 
ACCAGGAAGA AGGTGCTGCG GCTCTCAGGC TCTCTAGCCT CTATGGATCA GACTCTGCAG 
GACATCAACA GCATGTTCCT GCCCGGAGGA AGCCTGACCG ACACCTGGAC GCAGCAGGTG 
GGAACACACC CCGAGGACCG CAATGTGGAG AAGATCAAGG TCCTGCTGGA CGCCATCGGG 
GCCATTTACC AGCAGTTCAA GAAGGACAAG GCGGAGCGGC GTCTACCCTA TAATGAAGAG 
CAGATCCACA AGTTTGACAA GCAGAAGCTG GTCCTTCACG CCACTAAAGC GCGAGCTCTG 
TTCACAGACG AGTGTGCCAT GAAGTATCGC CTCTTCATCT CCAAGAGTGA GGAGTGGATG 
AAGAAGTTTC ATCACGTGCG GAAGCATCTC CTGTCTCTGA CTGGTCAGTT CAGCAGCCTG 
GAGCAGGAAG TGACTCTCCT CATGCAGCGC CTGTATAAGC TGCTGGAGCA GTTCCCACAG 
AAGGTGGTCC CGATGGCGTC TGGTGTCCTG AAGCCGCAGG CGTATCTGAG CCCCAGCACA 
CTCGTGGAGA TGACCCTTGG GATGAAGAAA CTGAAGGAGG AGATGGAGGG AGTGGTGAAG 
GAGCTGGCGG AGAACAACCT GTTCCTGGAG AGATTCGGCT CACTGACGGT GGATGGAGGA 
ATGAGGACAG TGGAGCGGAT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001038213.2
CDS	64..2247
Translation	MQSTANYLWMMSDLLGQGATANVYRGRHKKTGDLYAVKVFNNLSFLRPLDVQMREFEVLKKLNHKNIVKLFAVEEESNTRHKVLVMEYCPCGSLYTVLEEPTNAYGLPEDEFLIVLQDVVAGMNHLREYGIVHRDIKPGNIMRVIGDDGFSVYKLTDFGAARELEDDEQFVSLYGTEEYLHPDMYERAVLRKDHQKKYGATVDLWSIGVTFYHAATGSLPFRPFEGPRRNKEVMYKIITEKPPGAISGHQKFENGKIEWSSEMPISCSLSKGLQSLLTPVLANILEADQEKCWGFDQFFAETSDILHRIVVYVFSLQQATLHHVYIHTYNTANLFQELLFRRTNITPSHQELLYEGRRLVLDPNRQAQTFPKTSRDNPIMLLCRDPVNTVGLLFEDPSPPKVQPRYDLDLDASYAKTFAGDVGYLWKTSDSLLLYQELVRKGVRGLNELIRDEYSETMHKKTEVFHLCSHCSQTLERSEQLCEALMQGNILSAEYDEIRDTRKKVLRLSGSLASMDQTLQDINSMFLPGGSLTDTWTQQVGTHPEDRNVEKIKVLLDAIGAIYQQFKKDKAERRLPYNEEQIHKFDKQKLVLHATKARALFTDECAMKYRLFISKSEEWMKKFHHVRKHLLSLTGQFSSLEQEVTLLMQRLYKLLEQFPQKVVPMASGVLKPQAYLSPSTLVEMTLGMKKLKEEMEGVVKELAENNLFLERFGSLTVDGGMRTVERM

Target ORF information:

RefSeq Version	NM_001044748.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio TANK-binding kinase 1 (tbk1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001044748.2

ORF Insert Sequence:

ATGCAGAGTA CGGCCAATTA CCTGTGGATG ATGTCCGACC TGCTGGGTCA GGGAGCCACA 
GCTAACGTGT ACCGCGGTCG ACACAAGAAA ACAGGCGATC TGTACGCCGT CAAAGTGTTC 
AACAACCTGA GTTTCCTGCG GCCGCTGGAC GTGCAGATGA GGGAGTTTGA GGTGCTGAAG 
AAACTCAATC ACAAGAACAT CGTCAAGCTG TTCGCCGTCG AGGAGGAGTC GAACACACGT 
CATAAGGTGC TGGTGATGGA GTACTGCCCC TGCGGGAGTC TCTACACCGT CCTGGAGGAG 
CCCACCAATG CGTACGGCCT GCCGGAGGAC GAGTTCCTCA TCGTTCTGCA GGACGTCGTG 
GCGGGAATGA ATCACCTGCG CGAGTACGGC ATCGTGCATC GAGACATCAA GCCAGGGAAC 
ATCATGCGAG TGATTGGAGA CGACGGATTC TCTGTGTACA AACTCACCGA CTTCGGAGCC 
GCTCGCGAAC TGGAGGACGA CGAGCAGTTC GTATCGCTGT ACGGCACAGA GGAGTATCTG 
CATCCAGACA TGTACGAGCG GGCGGTGCTG AGGAAAGACC ACCAGAAGAA ATACGGCGCT 
ACGGTGGATC TGTGGAGCAT CGGAGTCACG TTTTACCACG CCGCCACCGG CAGCCTGCCG 
TTCAGACCCT TCGAAGGGCC GCGCAGAAAC AAAGAAGTCA TGTATAAGAT CATCACAGAG 
AAGCCCCCCG GCGCCATCTC CGGACACCAG AAGTTTGAAA ATGGGAAGAT CGAGTGGAGC 
TCCGAGATGC CCATCTCCTG CAGCCTGTCC AAGGGTCTGC AGAGCCTCCT GACGCCGGTG 
CTGGCGAATA TCCTGGAAGC GGATCAGGAG AAGTGCTGGG GGTTTGATCA GTTCTTCGCA 
GAGACCAGCG ACATCTTACA CCGCATCGTG GTCTACGTCT TCAGTCTGCA GCAGGCCACG 
CTGCACCACG TCTACATACA CACTTACAAC ACAGCGAACC TCTTCCAGGA GCTGCTGTTC 
AGACGCACCA ACATCACTCC GTCCCATCAG GAGCTGCTCT ACGAGGGCCG ACGGCTCGTC 
CTCGACCCCA ACCGGCAAGC ACAAACCTTT CCCAAGACCT CCAGAGACAA TCCCATCATG 
CTCCTGTGCA GAGACCCCGT CAACACCGTC GGCCTGCTGT TTGAGGACCC CAGCCCACCC 
AAAGTACAGC CGCGCTACGA CCTGGACCTG GACGCCAGCT ACGCCAAGAC GTTCGCAGGT 
GATGTGGGAT ACCTGTGGAA AACATCAGAT TCTCTGCTTC TGTACCAGGA GCTGGTGAGG 
AAAGGAGTCC GAGGCCTGAA TGAGCTGATC AGAGATGAGT ACAGTGAGAC GATGCACAAG 
AAGACGGAGG TGTTCCACCT GTGCAGCCAC TGCAGCCAGA CACTGGAGCG CAGCGAACAA 
CTCTGTGAGG CGCTGATGCA GGGAAATATC CTGTCAGCCG AGTATGACGA GATCCGAGAC 
ACCAGGAAGA AGGTGCTGCG GCTCTCAGGC TCTCTAGCCT CTATGGATCA GACTCTGCAG 
GACATCAACA GCATGTTCCT GCCCGGAGGA AGCCTGACCG ACACCTGGAC GCAGCAGGTG 
GGAACACACC CCGAGGACCG CAATGTGGAG AAGATCAAGG TCCTGCTGGA CGCCATCGGG 
GCCATTTACC AGCAGTTCAA GAAGGACAAG GCGGAGCGGC GTCTACCCTA TAATGAAGAG 
CAGATCCACA AGTTTGACAA GCAGAAGCTG GTCCTTCACG CCACTAAAGC GCGAGCTCTG 
TTCACAGACG AGTGTGCCAT GAAGTATCGC CTCTTCATCT CCAAGAGTGA GGAGTGGATG 
AAGAAGTTTC ATCACGTGCG GAAGCATCTC CTGTCTCTGA CTGGTCAGTT CAGCAGCCTG 
GAGCAGGAAG TGACTCTCCT CATGCAGCGC CTGTATAAGC TGCTGGAGCA GTTCCCACAG 
AAGGTGGTCC CGATGGCGTC TGGTGTCCTG AAGCCGCAGG CGTATCTGAG CCCCAGCACA 
CTCGTGGAGA TGACCCTTGG GATGAAGAAA CTGAAGGAGG AGATGGAGGG AGTGGTGAAG 
GAGCTGGCGG AGAACAACCT GTTCCTGGAG AGATTCGGCT CACTGACGGT GGATGGAGGA 
ATGAGGACAG TGGAGCGGAT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tbk1 ( XM_021475140.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021330815.1 Danio rerio TANK-binding kinase 1 (tbk1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa31635
Clone ID Related Accession (Same CDS sequence)	NM_001044748.2 , XM_021475140.1
Accession Version	XM_021475140.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2184bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	serine/threonine-protein kinase TBK1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGAGTA CGGCCAATTA CCTGTGGATG ATGTCCGACC TGCTGGGTCA GGGAGCCACA 
GCTAACGTGT ACCGCGGTCG ACACAAGAAA ACAGGCGATC TGTACGCCGT CAAAGTGTTC 
AACAACCTGA GTTTCCTGCG GCCGCTGGAC GTGCAGATGA GGGAGTTTGA GGTGCTGAAG 
AAACTCAATC ACAAGAACAT CGTCAAGCTG TTCGCCGTCG AGGAGGAGTC GAACACACGT 
CATAAGGTGC TGGTGATGGA GTACTGCCCC TGCGGGAGTC TCTACACCGT CCTGGAGGAG 
CCCACCAATG CGTACGGCCT GCCGGAGGAC GAGTTCCTCA TCGTTCTGCA GGACGTCGTG 
GCGGGAATGA ATCACCTGCG CGAGTACGGC ATCGTGCATC GAGACATCAA GCCAGGGAAC 
ATCATGCGAG TGATTGGAGA CGACGGATTC TCTGTGTACA AACTCACCGA CTTCGGAGCC 
GCTCGCGAAC TGGAGGACGA CGAGCAGTTC GTATCGCTGT ACGGCACAGA GGAGTATCTG 
CATCCAGACA TGTACGAGCG GGCGGTGCTG AGGAAAGACC ACCAGAAGAA ATACGGCGCT 
ACGGTGGATC TGTGGAGCAT CGGAGTCACG TTTTACCACG CCGCCACCGG CAGCCTGCCG 
TTCAGACCCT TCGAAGGGCC GCGCAGAAAC AAAGAAGTCA TGTATAAGAT CATCACAGAG 
AAGCCCCCCG GCGCCATCTC CGGACACCAG AAGTTTGAAA ATGGGAAGAT CGAGTGGAGC 
TCCGAGATGC CCATCTCCTG CAGCCTGTCC AAGGGTCTGC AGAGCCTCCT GACGCCGGTG 
CTGGCGAATA TCCTGGAAGC GGATCAGGAG AAGTGCTGGG GGTTTGATCA GTTCTTCGCA 
GAGACCAGCG ACATCTTACA CCGCATCGTG GTCTACGTCT TCAGTCTGCA GCAGGCCACG 
CTGCACCACG TCTACATACA CACTTACAAC ACAGCGAACC TCTTCCAGGA GCTGCTGTTC 
AGACGCACCA ACATCACTCC GTCCCATCAG GAGCTGCTCT ACGAGGGCCG ACGGCTCGTC 
CTCGACCCCA ACCGGCAAGC ACAAACCTTT CCCAAGACCT CCAGAGACAA TCCCATCATG 
CTCCTGTGCA GAGACCCCGT CAACACCGTC GGCCTGCTGT TTGAGGACCC CAGCCCACCC 
AAAGTACAGC CGCGCTACGA CCTGGACCTG GACGCCAGCT ACGCCAAGAC GTTCGCAGGT 
GATGTGGGAT ACCTGTGGAA AACATCAGAT TCTCTGCTTC TGTACCAGGA GCTGGTGAGG 
AAAGGAGTCC GAGGCCTGAA TGAGCTGATC AGAGATGAGT ACAGTGAGAC GATGCACAAG 
AAGACGGAGG TGTTCCACCT GTGCAGCCAC TGCAGCCAGA CACTGGAGCG CAGCGAACAA 
CTCTGTGAGG CGCTGATGCA GGGAAATATC CTGTCAGCCG AGTATGACGA GATCCGAGAC 
ACCAGGAAGA AGGTGCTGCG GCTCTCAGGC TCTCTAGCCT CTATGGATCA GACTCTGCAG 
GACATCAACA GCATGTTCCT GCCCGGAGGA AGCCTGACCG ACACCTGGAC GCAGCAGGTG 
GGAACACACC CCGAGGACCG CAATGTGGAG AAGATCAAGG TCCTGCTGGA CGCCATCGGG 
GCCATTTACC AGCAGTTCAA GAAGGACAAG GCGGAGCGGC GTCTACCCTA TAATGAAGAG 
CAGATCCACA AGTTTGACAA GCAGAAGCTG GTCCTTCACG CCACTAAAGC GCGAGCTCTG 
TTCACAGACG AGTGTGCCAT GAAGTATCGC CTCTTCATCT CCAAGAGTGA GGAGTGGATG 
AAGAAGTTTC ATCACGTGCG GAAGCATCTC CTGTCTCTGA CTGGTCAGTT CAGCAGCCTG 
GAGCAGGAAG TGACTCTCCT CATGCAGCGC CTGTATAAGC TGCTGGAGCA GTTCCCACAG 
AAGGTGGTCC CGATGGCGTC TGGTGTCCTG AAGCCGCAGG CGTATCTGAG CCCCAGCACA 
CTCGTGGAGA TGACCCTTGG GATGAAGAAA CTGAAGGAGG AGATGGAGGG AGTGGTGAAG 
GAGCTGGCGG AGAACAACCT GTTCCTGGAG AGATTCGGCT CACTGACGGT GGATGGAGGA 
ATGAGGACAG TGGAGCGGAT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021330815.1
CDS	37..2220
Translation	MQSTANYLWMMSDLLGQGATANVYRGRHKKTGDLYAVKVFNNLSFLRPLDVQMREFEVLKKLNHKNIVKLFAVEEESNTRHKVLVMEYCPCGSLYTVLEEPTNAYGLPEDEFLIVLQDVVAGMNHLREYGIVHRDIKPGNIMRVIGDDGFSVYKLTDFGAARELEDDEQFVSLYGTEEYLHPDMYERAVLRKDHQKKYGATVDLWSIGVTFYHAATGSLPFRPFEGPRRNKEVMYKIITEKPPGAISGHQKFENGKIEWSSEMPISCSLSKGLQSLLTPVLANILEADQEKCWGFDQFFAETSDILHRIVVYVFSLQQATLHHVYIHTYNTANLFQELLFRRTNITPSHQELLYEGRRLVLDPNRQAQTFPKTSRDNPIMLLCRDPVNTVGLLFEDPSPPKVQPRYDLDLDASYAKTFAGDVGYLWKTSDSLLLYQELVRKGVRGLNELIRDEYSETMHKKTEVFHLCSHCSQTLERSEQLCEALMQGNILSAEYDEIRDTRKKVLRLSGSLASMDQTLQDINSMFLPGGSLTDTWTQQVGTHPEDRNVEKIKVLLDAIGAIYQQFKKDKAERRLPYNEEQIHKFDKQKLVLHATKARALFTDECAMKYRLFISKSEEWMKKFHHVRKHLLSLTGQFSSLEQEVTLLMQRLYKLLEQFPQKVVPMASGVLKPQAYLSPSTLVEMTLGMKKLKEEMEGVVKELAENNLFLERFGSLTVDGGMRTVERM

Target ORF information:

RefSeq Version	XM_021475140.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio TANK-binding kinase 1 (tbk1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021475140.1

ORF Insert Sequence:

ATGCAGAGTA CGGCCAATTA CCTGTGGATG ATGTCCGACC TGCTGGGTCA GGGAGCCACA 
GCTAACGTGT ACCGCGGTCG ACACAAGAAA ACAGGCGATC TGTACGCCGT CAAAGTGTTC 
AACAACCTGA GTTTCCTGCG GCCGCTGGAC GTGCAGATGA GGGAGTTTGA GGTGCTGAAG 
AAACTCAATC ACAAGAACAT CGTCAAGCTG TTCGCCGTCG AGGAGGAGTC GAACACACGT 
CATAAGGTGC TGGTGATGGA GTACTGCCCC TGCGGGAGTC TCTACACCGT CCTGGAGGAG 
CCCACCAATG CGTACGGCCT GCCGGAGGAC GAGTTCCTCA TCGTTCTGCA GGACGTCGTG 
GCGGGAATGA ATCACCTGCG CGAGTACGGC ATCGTGCATC GAGACATCAA GCCAGGGAAC 
ATCATGCGAG TGATTGGAGA CGACGGATTC TCTGTGTACA AACTCACCGA CTTCGGAGCC 
GCTCGCGAAC TGGAGGACGA CGAGCAGTTC GTATCGCTGT ACGGCACAGA GGAGTATCTG 
CATCCAGACA TGTACGAGCG GGCGGTGCTG AGGAAAGACC ACCAGAAGAA ATACGGCGCT 
ACGGTGGATC TGTGGAGCAT CGGAGTCACG TTTTACCACG CCGCCACCGG CAGCCTGCCG 
TTCAGACCCT TCGAAGGGCC GCGCAGAAAC AAAGAAGTCA TGTATAAGAT CATCACAGAG 
AAGCCCCCCG GCGCCATCTC CGGACACCAG AAGTTTGAAA ATGGGAAGAT CGAGTGGAGC 
TCCGAGATGC CCATCTCCTG CAGCCTGTCC AAGGGTCTGC AGAGCCTCCT GACGCCGGTG 
CTGGCGAATA TCCTGGAAGC GGATCAGGAG AAGTGCTGGG GGTTTGATCA GTTCTTCGCA 
GAGACCAGCG ACATCTTACA CCGCATCGTG GTCTACGTCT TCAGTCTGCA GCAGGCCACG 
CTGCACCACG TCTACATACA CACTTACAAC ACAGCGAACC TCTTCCAGGA GCTGCTGTTC 
AGACGCACCA ACATCACTCC GTCCCATCAG GAGCTGCTCT ACGAGGGCCG ACGGCTCGTC 
CTCGACCCCA ACCGGCAAGC ACAAACCTTT CCCAAGACCT CCAGAGACAA TCCCATCATG 
CTCCTGTGCA GAGACCCCGT CAACACCGTC GGCCTGCTGT TTGAGGACCC CAGCCCACCC 
AAAGTACAGC CGCGCTACGA CCTGGACCTG GACGCCAGCT ACGCCAAGAC GTTCGCAGGT 
GATGTGGGAT ACCTGTGGAA AACATCAGAT TCTCTGCTTC TGTACCAGGA GCTGGTGAGG 
AAAGGAGTCC GAGGCCTGAA TGAGCTGATC AGAGATGAGT ACAGTGAGAC GATGCACAAG 
AAGACGGAGG TGTTCCACCT GTGCAGCCAC TGCAGCCAGA CACTGGAGCG CAGCGAACAA 
CTCTGTGAGG CGCTGATGCA GGGAAATATC CTGTCAGCCG AGTATGACGA GATCCGAGAC 
ACCAGGAAGA AGGTGCTGCG GCTCTCAGGC TCTCTAGCCT CTATGGATCA GACTCTGCAG 
GACATCAACA GCATGTTCCT GCCCGGAGGA AGCCTGACCG ACACCTGGAC GCAGCAGGTG 
GGAACACACC CCGAGGACCG CAATGTGGAG AAGATCAAGG TCCTGCTGGA CGCCATCGGG 
GCCATTTACC AGCAGTTCAA GAAGGACAAG GCGGAGCGGC GTCTACCCTA TAATGAAGAG 
CAGATCCACA AGTTTGACAA GCAGAAGCTG GTCCTTCACG CCACTAAAGC GCGAGCTCTG 
TTCACAGACG AGTGTGCCAT GAAGTATCGC CTCTTCATCT CCAAGAGTGA GGAGTGGATG 
AAGAAGTTTC ATCACGTGCG GAAGCATCTC CTGTCTCTGA CTGGTCAGTT CAGCAGCCTG 
GAGCAGGAAG TGACTCTCCT CATGCAGCGC CTGTATAAGC TGCTGGAGCA GTTCCCACAG 
AAGGTGGTCC CGATGGCGTC TGGTGTCCTG AAGCCGCAGG CGTATCTGAG CCCCAGCACA 
CTCGTGGAGA TGACCCTTGG GATGAAGAAA CTGAAGGAGG AGATGGAGGG AGTGGTGAAG 
GAGCTGGCGG AGAACAACCT GTTCCTGGAG AGATTCGGCT CACTGACGGT GGATGGAGGA 
ATGAGGACAG TGGAGCGGAT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 Mar)
Bay?s ?,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

Zebra Fish Lacking Adaptive Immunity Acquire an Antiviral Alert State Characterized by Upregulated Gene Expression of Apoptosis, Multigene Families, and Interferon-Related Genes.
Frontiers in immunology8121(2017)
Garc?a-Valtanen P,Mart?nez-L?pez A,L?pez-Mu?oz A,Bello-Perez M,Medina-Gali RM,Ortega-Villaiz?n MD,Varela M,Figueras A,Mulero V,Novoa B,Estepa A,Coll J

FTR83, a Member of the Large Fish-Specific finTRIM Family, Triggers IFN Pathway and Counters Viral Infection.
Frontiers in immunology8617(2017)
Langevin C,Aleksejeva E,Houel A,Briolat V,Torhy C,Lunazzi A,Levraud JP,Boudinot P

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tbk1 cDNA ORF clone, Danio rerio(zebrafish)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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