eomesa cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	eomesa
Entrez Gene ID	64603
Full Name	eomesodermin homolog a
Synonyms	eom,eomes
General protein information	Preferred Names eomesodermin homolog a Names eomesodermin homolog eom-a eomes1 tbr2 tbr2a
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005159321.3	XP_005159378.1	eomesodermin homolog isoform X1
XM_005159319.3	XP_005159376.1	eomesodermin homolog isoform X1
XM_005159319.4	XP_005159376.1	eomesodermin homolog isoform X1
XM_005159321.4	XP_005159378.1	eomesodermin homolog isoform X1
NM_131679.3	NP_571754.3	eomesodermin homolog

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	eomesa	NP_571754.3
Canis lupus familiaris (dog)	EOMES	XP_005634394.1
Mus musculus (house mouse)	Eomes	NP_034266.2
Gallus gallus (chicken)	EOMES	XP_004939476.1
Homo sapiens (human)	EOMES	NP_001265111.1
Xenopus tropicalis (tropical clawed frog)	eomes	NP_001122124.1
Pan troglodytes (chimpanzee)	EOMES	XP_526157.4
Bos taurus (cattle)	EOMES	NP_001178117.1
Rattus norvegicus (Norway rat)	Eomes	XP_006244201.1

Gene Ontology
Bibliography

Related articles in PubMed

Maternal Nanog is required for zebrafish embryo architecture and for cell viability during gastrulation.
Veil M, Schaechtle MA, Gao M, Kirner V, Buryanova L, Grethen R, Onichtchouk D
Development (Cambridge, England)145(1)(2018 Jan)

Zebrafish Regulatory T Cells Mediate Organ-Specific Regenerative Programs.
Hui SP, Sheng DZ, Sugimoto K, Gonzalez-Rajal A, Nakagawa S, Hesselson D, Kikuchi K
Developmental cell43(6)659-672.e5(2017 Dec)

Zebrafish FOXP3 is required for the maintenance of immune tolerance.
Sugimoto K, Hui SP, Sheng DZ, Nakayama M, Kikuchi K
Developmental and comparative immunology73156-162(2017 Aug)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Smad2 and Eomesodermin a (Eomesa) bind common genomic regions proximal to genes involved in mesoderm and endoderm formation, suggesting Eomesa forms a general component of the Smad2 signalling complex in zebrafish.
Title: Global identification of Smad2 and Eomesodermin targets in zebrafish identifies a conserved transcriptional network in mesendoderm and a novel role for Eomesodermin in repression of ectodermal gene expression.

Eomesa has a strictly maternal role in the initiation of epiboly, and is required for normal expression of the endoderm markers sox32, bon and og9x; however it is not essential for endoderm formation.
Title: Differential regulation of epiboly initiation and progression by zebrafish Eomesodermin A.

Eomes is a Nodal-transducing factor that acts, directly and indirectly, in concert with FoxH1 to carry out all Nodal-dependent processes during early mesendoderm specification.
Title: Nodal-dependent mesendoderm specification requires the combinatorial activities of FoxH1 and Eomesodermin.

These results suggest that both Eomes genes are involved in the zebrafish immune response, particularly in lymphocyte function as has been found in mammals.
Title: Expression analysis of two Eomesodermin homologues in zebrafish lymphoid tissues and cells.

Results suggest that eomesodermin promotes endoderm induction in marginal blastomeres by facilitating the assembly of a transcriptional activating complex on the casanova promoter.
Title: Eomesodermin is a localized maternal determinant required for endoderm induction in zebrafish.

The following eomesa gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the eomesa cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa00796	NM_131679.3 Latest version!	Danio rerio eomesodermin homolog a (eomesa), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
ODa23698	XM_005159321.4 Latest version!	Danio rerio eomesodermin homolog a (eomesa), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
ODa23698	XM_005159319.4 Latest version!	Danio rerio eomesodermin homolog a (eomesa), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
View Old Accession Versions >>
ODa23698	XM_005159319.3	Danio rerio eomesodermin homolog a (eomesa), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
ODa23698	XM_005159321.3	Danio rerio eomesodermin homolog a (eomesa), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

eomesa ( NM_131679.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_571754.3 Danio rerio eomesodermin homolog a (eomesa), mRNA.

Price & Availability↑

CloneID	ODa00796
Clone ID Related Accession (Same CDS sequence)	NM_131679.3
Accession Version	NM_131679.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1986bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-14
Organism	Danio rerio(zebrafish)
Product	eomesodermin homolog
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC067719.1. On Apr 5, 2004 this sequence version replaced NM_131679.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC067719.1, EG576638.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA4476772 [ECO:0000348] ##Evidence-Data-END##

ATGCAGTTAG AAAGCATCCT CCCTGGCGCA TCTGTAAACT TACCCAAGAC CTTCTACAAC 
CTGTCCTCGT CCTCCGAAAG CACCAATAAT AGCCCGGGGT CAACGCAGAT CGACTTTCAG 
GACATGGACC GGACGGATTC GGAGCAGAGT TCTGGTGCCA AGAAGTTTCT GATGGATGAT 
GCTGACAGTG AGAGCTTTAC CGGGACTAAA GCAGCAGCAG CAGCCACCGC CGCACCGGAC 
GCGCGTAAAA GTTCTCCGGT GATCGGCGCG GATGATGAGC TCTCCAGCAC CCGCAGATAC 
AACATCGATG ATCTGGGCAC TGATCGATAC TTCATATCGT CCTCTCAACC GAGTTCTGAC 
GTGGCCAACC CTTGCTCTCT GTTCCCGTAC GGAGGGCAGA CCGGGTCGGT GTACAGCGGC 
TCGAATGGCT CCCGGTATTC GTCGTCTCTG CATTACGGTT CGGTTCTTCC ACCCGCCGGG 
TTCTCATCCG CCGTGTGCGC CAGCAGGAGT CAGTTTGGGG GTGGTTATCA GTTCGGACAG 
GGTCCCGGGT GTCTGTACCC GTCCTATCCC GGTCCAGGCT CCAGTCTCAG CTCGATGCCC 
ATTCCAGGTT CGGGCTCCGC GGCGCGGGCG CAGGTCTACC TGTGCAACAG ACCGCTGTGG 
CTCAAATTTC ACCGCCATCA AACTGAGATG ATCATAACTA AACAGGGCAG GCGAATGTTC 
CCCTTTCTGA GTTTTAATAT CACTGGACTG AACCTGACTG CGCATTATAA TGTGTTTGTG 
GAGATTGTTC TGGCCGATCC GAACCACTGG AGATTTCAGG GCGGAAAGTG GGTGACCTGC 
GGGAAAGCGG ACAACAACAT GCAAGGAAAC AAGGTTTATG TTCACCCAGA GTCACCAAAC 
ACTGGAGCGC ACTGGATGAG ACAAGAAATA TCTTTCGGAA AACTGAAGCT AACCAACAAC 
AAAGGAGCAA ATAACAACAA TACTCAGATG ATTGTCCTGC AGTCCCTGCA TAAGTACCAG 
CCTCGGCTTC ACATTGTGGA GGTGACTGAG GACGGTGTGG AGGACATGAG CAGCGAAGCT 
AAAACACAGA CCTTCACTTT CCCTGAAAAC CAGTTCATCG CTGTAACGGC TTACCAAAAC 
ACAGACATCA CACAGCTCAA GATCGACCAC AACCCCTTTG CAAAAGGCTT CAGGGACAAT 
TATGATTCGA TGTACACAGC TCCAGAGAGC GACAGACTGA CGCCCTCTCC TACTGACTCT 
CCGCGGTCGC ACCAGATTGT CCCCGGCGCC CGCTACGCAA TGCAGCCCTT CTTCCAAGAC 
CAATTTGTCA ACAACCTTCC CCAAAACCAC CGATTCTACG GCAGCGAGCG CGCCGTTCCC 
CAAACCAACG GCCTGCTGTC TCCTCAGAGC GAAGATTCGG CCGCGGGCTC GGCGTCAGCT 
CAGCGCTGGT TTGTGGGGCC GGTGCAACAA AGTGGTGCGA CCACCAAACT GGATCTGTCC 
TATGACAGTG AGTACTCTGC CTCCAGTCTG CTTCCCTACG GCATCAAACC ACTGCCGCTC 
CAGAGTCCAC ATGCACTCGG CTACTATCCT GATTCTGCAT TCGCATCTAT GGCAGCCGGA 
TGGAGCAGCA GAAGTTCCTA CCAGAGAAAA ATGACCACCG GCCTGCCGTG GTCACCCCGA 
CCAAGCCCTC CGGCATACAC CGATGACCTG CTGTCCTCCA AAGACAAGCT GCAGGATGAA 
AACTCCACAG CGGCCCCTAC GGCTGCCAGC ACAGGCCCCT GGATGGAGGC ACCTCCGGTG 
CTGAAAAGCG TGGATTCAGG CGACACCAGC GGTTACTCCA TGGCCTGTAA GCGGCGGCGT 
GTTTCGCCTG GAGGCTCCAG TACAGACGCT TCACCCACTA TAAAGTGTGA GGACTTGAGC 
TCTGAGGAGT ACAACAAAGA GAGCCATAAA GCCATGGGTT ATTACGCCTT CTACACCAGC 
CCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_571754.3
CDS	133..2118
Translation	MQLESILPGASVNLPKTFYNLSSSSESTNNSPGSTQIDFQDMDRTDSEQSSGAKKFLMDDADSESFTGTKAAAAATAAPDARKSSPVIGADDELSSTRRYNIDDLGTDRYFISSSQPSSDVANPCSLFPYGGQTGSVYSGSNGSRYSSSLHYGSVLPPAGFSSAVCASRSQFGGGYQFGQGPGCLYPSYPGPGSSLSSMPIPGSGSAARAQVYLCNRPLWLKFHRHQTEMIITKQGRRMFPFLSFNITGLNLTAHYNVFVEIVLADPNHWRFQGGKWVTCGKADNNMQGNKVYVHPESPNTGAHWMRQEISFGKLKLTNNKGANNNNTQMIVLQSLHKYQPRLHIVEVTEDGVEDMSSEAKTQTFTFPENQFIAVTAYQNTDITQLKIDHNPFAKGFRDNYDSMYTAPESDRLTPSPTDSPRSHQIVPGARYAMQPFFQDQFVNNLPQNHRFYGSERAVPQTNGLLSPQSEDSAAGSASAQRWFVGPVQQSGATTKLDLSYDSEYSASSLLPYGIKPLPLQSPHALGYYPDSAFASMAAGWSSRSSYQRKMTTGLPWSPRPSPPAYTDDLLSSKDKLQDENSTAAPTAASTGPWMEAPPVLKSVDSGDTSGYSMACKRRRVSPGGSSTDASPTIKCEDLSSEEYNKESHKAMGYYAFYTSP

Target ORF information:

RefSeq Version	NM_131679.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio eomesodermin homolog a (eomesa), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_131679.3

ORF Insert Sequence:

ATGCAGTTAG AAAGCATCCT CCCTGGCGCA TCTGTAAACT TACCCAAGAC CTTCTACAAC 
CTGTCCTCGT CCTCCGAAAG CACCAATAAT AGCCCGGGGT CAACGCAGAT CGACTTTCAG 
GACATGGACC GGACGGATTC GGAGCAGAGT TCTGGTGCCA AGAAGTTTCT GATGGATGAT 
GCTGACAGTG AGAGCTTTAC CGGGACTAAA GCAGCAGCAG CAGCCACCGC CGCACCGGAC 
GCGCGTAAAA GTTCTCCGGT GATCGGCGCG GATGATGAGC TCTCCAGCAC CCGCAGATAC 
AACATCGATG ATCTGGGCAC TGATCGATAC TTCATATCGT CCTCTCAACC GAGTTCTGAC 
GTGGCCAACC CTTGCTCTCT GTTCCCGTAC GGAGGGCAGA CCGGGTCGGT GTACAGCGGC 
TCGAATGGCT CCCGGTATTC GTCGTCTCTG CATTACGGTT CGGTTCTTCC ACCCGCCGGG 
TTCTCATCCG CCGTGTGCGC CAGCAGGAGT CAGTTTGGGG GTGGTTATCA GTTCGGACAG 
GGTCCCGGGT GTCTGTACCC GTCCTATCCC GGTCCAGGCT CCAGTCTCAG CTCGATGCCC 
ATTCCAGGTT CGGGCTCCGC GGCGCGGGCG CAGGTCTACC TGTGCAACAG ACCGCTGTGG 
CTCAAATTTC ACCGCCATCA AACTGAGATG ATCATAACTA AACAGGGCAG GCGAATGTTC 
CCCTTTCTGA GTTTTAATAT CACTGGACTG AACCTGACTG CGCATTATAA TGTGTTTGTG 
GAGATTGTTC TGGCCGATCC GAACCACTGG AGATTTCAGG GCGGAAAGTG GGTGACCTGC 
GGGAAAGCGG ACAACAACAT GCAAGGAAAC AAGGTTTATG TTCACCCAGA GTCACCAAAC 
ACTGGAGCGC ACTGGATGAG ACAAGAAATA TCTTTCGGAA AACTGAAGCT AACCAACAAC 
AAAGGAGCAA ATAACAACAA TACTCAGATG ATTGTCCTGC AGTCCCTGCA TAAGTACCAG 
CCTCGGCTTC ACATTGTGGA GGTGACTGAG GACGGTGTGG AGGACATGAG CAGCGAAGCT 
AAAACACAGA CCTTCACTTT CCCTGAAAAC CAGTTCATCG CTGTAACGGC TTACCAAAAC 
ACAGACATCA CACAGCTCAA GATCGACCAC AACCCCTTTG CAAAAGGCTT CAGGGACAAT 
TATGATTCGA TGTACACAGC TCCAGAGAGC GACAGACTGA CGCCCTCTCC TACTGACTCT 
CCGCGGTCGC ACCAGATTGT CCCCGGCGCC CGCTACGCAA TGCAGCCCTT CTTCCAAGAC 
CAATTTGTCA ACAACCTTCC CCAAAACCAC CGATTCTACG GCAGCGAGCG CGCCGTTCCC 
CAAACCAACG GCCTGCTGTC TCCTCAGAGC GAAGATTCGG CCGCGGGCTC GGCGTCAGCT 
CAGCGCTGGT TTGTGGGGCC GGTGCAACAA AGTGGTGCGA CCACCAAACT GGATCTGTCC 
TATGACAGTG AGTACTCTGC CTCCAGTCTG CTTCCCTACG GCATCAAACC ACTGCCGCTC 
CAGAGTCCAC ATGCACTCGG CTACTATCCT GATTCTGCAT TCGCATCTAT GGCAGCCGGA 
TGGAGCAGCA GAAGTTCCTA CCAGAGAAAA ATGACCACCG GCCTGCCGTG GTCACCCCGA 
CCAAGCCCTC CGGCATACAC CGATGACCTG CTGTCCTCCA AAGACAAGCT GCAGGATGAA 
AACTCCACAG CGGCCCCTAC GGCTGCCAGC ACAGGCCCCT GGATGGAGGC ACCTCCGGTG 
CTGAAAAGCG TGGATTCAGG CGACACCAGC GGTTACTCCA TGGCCTGTAA GCGGCGGCGT 
GTTTCGCCTG GAGGCTCCAG TACAGACGCT TCACCCACTA TAAAGTGTGA GGACTTGAGC 
TCTGAGGAGT ACAACAAAGA GAGCCATAAA GCCATGGGTT ATTACGCCTT CTACACCAGC 
CCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

eomesa ( XM_005159321.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005159378.1 Danio rerio eomesodermin homolog a (eomesa), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa23698
Clone ID Related Accession (Same CDS sequence)	XM_005159321.3 , XM_005159319.3 , XM_005159319.4 , XM_005159321.4
Accession Version	XM_005159321.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1986bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	eomesodermin homolog isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007130.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005159321.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGTTAG AAAGCATCCT CCCTGGCGCA TCTGTAAACT TACCCAAGAC CTTCTACAAC 
CTGTCCTCAT CCTCCGAAAG CACCAATAAT AGCCCGGGGT CAACGCAGAT CGACTTTCAG 
GACATGGACC GGACGGATTC GGAGCAGAGT TCTGGTGCCA AGAAGTTTCT GATGGATGAT 
GCTGACAGTG AGAGCTTTAC CGGGACTAAA GCAGCAGCAG CAGCCACCGC CGCACCGGAC 
GCGCGTAAAA GTTCTCCGGT GATCGGCGCG GATGATGAGC TCTCCAGCAC CCGCAGATAC 
AACATCGATG ATCTGGGCAC TGATCGATAC TTCATATCGT CCTCTCAACC GAGTTCTGAC 
GTGGCCAACC CGTGCTCTCT GTTCCCGTAC GGAGGGCAGA CCGGGTCGGT GTACAGCGGC 
TCGAATGGCT CCCGGTATTC GTCGTCTCTG CATTACGGTT CGGTTCTTCC ACCCGCCGGG 
TTCTCATCCG CCGTGTGCGC CAGCAGGAGT CAGTTTGGGG GTGGTTATCA GTTCGGACAG 
GGTCCCGGGT GTCTGTACCC GTCCTATCCC GGTCCAGGCT CCAGTCTCAG CTCGATGCCC 
ATTCCAGGTT CGGGCTCCGC GGCGCGGGCG CAGGTTTACC TGTGCAACAG ACCGCTGTGG 
CTCAAATTTC ACCGCCATCA AACTGAGATG ATCATAACTA AACAGGGCAG GCGAATGTTC 
CCCTTTCTGA GTTTTAATAT CACTGGACTG AACCTGACTG CGCATTATAA TGTGTTTGTG 
GAGATTGTTC TGGCCGATCC GAACCACTGG AGATTTCAGG GCGGAAAGTG GGTGACCTGC 
GGGAAAGCGG ACAACAACAT GCAAGGAAAC AAGGTGTATG TTCACCCAGA GTCACCAAAC 
ACTGGAGCGC ACTGGATGAG ACAAGAAATA TCTTTCGGAA AACTGAAGCT AACCAACAAC 
AAAGGAGCAA ATAACAACAA TACTCAGATG ATTGTCCTGC AGTCCCTGCA TAAGTACCAG 
CCTCGGCTTC ACATTGTGGA GGTGACTGAG GACGGTGTGG AGGACATGAG CAGCGAAGCT 
AAAACACAGA CCTTCACTTT CCCTGAAAAC CAGTTCATCG CTGTAACGGC TTACCAAAAC 
ACAGACATCA CACAGCTCAA GATCGACCAC AACCCCTTTG CAAAAGGCTT CAGGGACAAT 
TATGATTCGA TGTACACAGC TCCAGAGAGC GACAGACTGA CGCCCTCTCC TACTGACTCT 
CCGCGGTCGC ACCAGATTGT CCCCGGCGCC CGCTACGCAA TGCAGCCCTT CTTCCAAGAC 
CAATTTGTCA ACAACCTTCC CCAAAACCAC CGATTCTACG GCAGCGAGCG CGCCGTTCCC 
CAAACCAACG GCCTGCTGTC TCCTCAGAGC GAAGATTCGG CCGCGGGCTC GGCGTCAGCT 
CAGCGCTGGT TTGTGGGGCC GGTGCAACAA AGTGGTGCGA CCACCAAACT GGATCTGTCC 
TATGACAGTG AGTACTCTGC CTCCAGTCTG CTTCCCTACG GCATCAAACC ACTGCCGCTC 
CAGAGTCCAC ATGCACTCGG CTACTATCCT GATTCTGCAT TCGCATCTAT GGCAGCCGGA 
TGGAGCAGCA GAAGTTCCTA CCAGAGAAAA ATGACCACCG GCCTGCCGTG GTCACCCCGA 
CCAAGCCCTC CGGCATACAC CGATGACCTG CTGTCCTCCA AAGACAAGCT GCAGGATGAA 
AACTCCACAG CGGCCCCTAC GGCTGCCAGC ACAGGCCCCT GGATGGAGGC ACCTCCGGTG 
CTGAAAAGCG TGGATTCAGG CGACACCAGC GGTTACTCCA TGGCCTGTAA GCGGCGGCGT 
GTTTCGCCTG GAGGCTCCAG TACAGACGCT TCACCCACTA TAAAGTGTGA GGACTTGAGC 
TCTGAGGAGT ACAACAAAGA GAGCCATAAA GCCATGGGTT ATTACGCCTT CTACACCAGC 
CCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005159378.1
CDS	365..2350
Translation	MQLESILPGASVNLPKTFYNLSSSSESTNNSPGSTQIDFQDMDRTDSEQSSGAKKFLMDDADSESFTGTKAAAAATAAPDARKSSPVIGADDELSSTRRYNIDDLGTDRYFISSSQPSSDVANPCSLFPYGGQTGSVYSGSNGSRYSSSLHYGSVLPPAGFSSAVCASRSQFGGGYQFGQGPGCLYPSYPGPGSSLSSMPIPGSGSAARAQVYLCNRPLWLKFHRHQTEMIITKQGRRMFPFLSFNITGLNLTAHYNVFVEIVLADPNHWRFQGGKWVTCGKADNNMQGNKVYVHPESPNTGAHWMRQEISFGKLKLTNNKGANNNNTQMIVLQSLHKYQPRLHIVEVTEDGVEDMSSEAKTQTFTFPENQFIAVTAYQNTDITQLKIDHNPFAKGFRDNYDSMYTAPESDRLTPSPTDSPRSHQIVPGARYAMQPFFQDQFVNNLPQNHRFYGSERAVPQTNGLLSPQSEDSAAGSASAQRWFVGPVQQSGATTKLDLSYDSEYSASSLLPYGIKPLPLQSPHALGYYPDSAFASMAAGWSSRSSYQRKMTTGLPWSPRPSPPAYTDDLLSSKDKLQDENSTAAPTAASTGPWMEAPPVLKSVDSGDTSGYSMACKRRRVSPGGSSTDASPTIKCEDLSSEEYNKESHKAMGYYAFYTSP

Target ORF information:

RefSeq Version	XM_005159321.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio eomesodermin homolog a (eomesa), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005159321.3

ORF Insert Sequence:

ATGCAGTTAG AAAGCATCCT CCCTGGCGCA TCTGTAAACT TACCCAAGAC CTTCTACAAC 
CTGTCCTCAT CCTCCGAAAG CACCAATAAT AGCCCGGGGT CAACGCAGAT CGACTTTCAG 
GACATGGACC GGACGGATTC GGAGCAGAGT TCTGGTGCCA AGAAGTTTCT GATGGATGAT 
GCTGACAGTG AGAGCTTTAC CGGGACTAAA GCAGCAGCAG CAGCCACCGC CGCACCGGAC 
GCGCGTAAAA GTTCTCCGGT GATCGGCGCG GATGATGAGC TCTCCAGCAC CCGCAGATAC 
AACATCGATG ATCTGGGCAC TGATCGATAC TTCATATCGT CCTCTCAACC GAGTTCTGAC 
GTGGCCAACC CGTGCTCTCT GTTCCCGTAC GGAGGGCAGA CCGGGTCGGT GTACAGCGGC 
TCGAATGGCT CCCGGTATTC GTCGTCTCTG CATTACGGTT CGGTTCTTCC ACCCGCCGGG 
TTCTCATCCG CCGTGTGCGC CAGCAGGAGT CAGTTTGGGG GTGGTTATCA GTTCGGACAG 
GGTCCCGGGT GTCTGTACCC GTCCTATCCC GGTCCAGGCT CCAGTCTCAG CTCGATGCCC 
ATTCCAGGTT CGGGCTCCGC GGCGCGGGCG CAGGTTTACC TGTGCAACAG ACCGCTGTGG 
CTCAAATTTC ACCGCCATCA AACTGAGATG ATCATAACTA AACAGGGCAG GCGAATGTTC 
CCCTTTCTGA GTTTTAATAT CACTGGACTG AACCTGACTG CGCATTATAA TGTGTTTGTG 
GAGATTGTTC TGGCCGATCC GAACCACTGG AGATTTCAGG GCGGAAAGTG GGTGACCTGC 
GGGAAAGCGG ACAACAACAT GCAAGGAAAC AAGGTGTATG TTCACCCAGA GTCACCAAAC 
ACTGGAGCGC ACTGGATGAG ACAAGAAATA TCTTTCGGAA AACTGAAGCT AACCAACAAC 
AAAGGAGCAA ATAACAACAA TACTCAGATG ATTGTCCTGC AGTCCCTGCA TAAGTACCAG 
CCTCGGCTTC ACATTGTGGA GGTGACTGAG GACGGTGTGG AGGACATGAG CAGCGAAGCT 
AAAACACAGA CCTTCACTTT CCCTGAAAAC CAGTTCATCG CTGTAACGGC TTACCAAAAC 
ACAGACATCA CACAGCTCAA GATCGACCAC AACCCCTTTG CAAAAGGCTT CAGGGACAAT 
TATGATTCGA TGTACACAGC TCCAGAGAGC GACAGACTGA CGCCCTCTCC TACTGACTCT 
CCGCGGTCGC ACCAGATTGT CCCCGGCGCC CGCTACGCAA TGCAGCCCTT CTTCCAAGAC 
CAATTTGTCA ACAACCTTCC CCAAAACCAC CGATTCTACG GCAGCGAGCG CGCCGTTCCC 
CAAACCAACG GCCTGCTGTC TCCTCAGAGC GAAGATTCGG CCGCGGGCTC GGCGTCAGCT 
CAGCGCTGGT TTGTGGGGCC GGTGCAACAA AGTGGTGCGA CCACCAAACT GGATCTGTCC 
TATGACAGTG AGTACTCTGC CTCCAGTCTG CTTCCCTACG GCATCAAACC ACTGCCGCTC 
CAGAGTCCAC ATGCACTCGG CTACTATCCT GATTCTGCAT TCGCATCTAT GGCAGCCGGA 
TGGAGCAGCA GAAGTTCCTA CCAGAGAAAA ATGACCACCG GCCTGCCGTG GTCACCCCGA 
CCAAGCCCTC CGGCATACAC CGATGACCTG CTGTCCTCCA AAGACAAGCT GCAGGATGAA 
AACTCCACAG CGGCCCCTAC GGCTGCCAGC ACAGGCCCCT GGATGGAGGC ACCTCCGGTG 
CTGAAAAGCG TGGATTCAGG CGACACCAGC GGTTACTCCA TGGCCTGTAA GCGGCGGCGT 
GTTTCGCCTG GAGGCTCCAG TACAGACGCT TCACCCACTA TAAAGTGTGA GGACTTGAGC 
TCTGAGGAGT ACAACAAAGA GAGCCATAAA GCCATGGGTT ATTACGCCTT CTACACCAGC 
CCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

eomesa ( XM_005159319.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005159376.1 Danio rerio eomesodermin homolog a (eomesa), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa23698
Clone ID Related Accession (Same CDS sequence)	XM_005159321.3 , XM_005159319.3 , XM_005159319.4 , XM_005159321.4
Accession Version	XM_005159319.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1986bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	eomesodermin homolog isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007130.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005159319.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGTTAG AAAGCATCCT CCCTGGCGCA TCTGTAAACT TACCCAAGAC CTTCTACAAC 
CTGTCCTCAT CCTCCGAAAG CACCAATAAT AGCCCGGGGT CAACGCAGAT CGACTTTCAG 
GACATGGACC GGACGGATTC GGAGCAGAGT TCTGGTGCCA AGAAGTTTCT GATGGATGAT 
GCTGACAGTG AGAGCTTTAC CGGGACTAAA GCAGCAGCAG CAGCCACCGC CGCACCGGAC 
GCGCGTAAAA GTTCTCCGGT GATCGGCGCG GATGATGAGC TCTCCAGCAC CCGCAGATAC 
AACATCGATG ATCTGGGCAC TGATCGATAC TTCATATCGT CCTCTCAACC GAGTTCTGAC 
GTGGCCAACC CGTGCTCTCT GTTCCCGTAC GGAGGGCAGA CCGGGTCGGT GTACAGCGGC 
TCGAATGGCT CCCGGTATTC GTCGTCTCTG CATTACGGTT CGGTTCTTCC ACCCGCCGGG 
TTCTCATCCG CCGTGTGCGC CAGCAGGAGT CAGTTTGGGG GTGGTTATCA GTTCGGACAG 
GGTCCCGGGT GTCTGTACCC GTCCTATCCC GGTCCAGGCT CCAGTCTCAG CTCGATGCCC 
ATTCCAGGTT CGGGCTCCGC GGCGCGGGCG CAGGTTTACC TGTGCAACAG ACCGCTGTGG 
CTCAAATTTC ACCGCCATCA AACTGAGATG ATCATAACTA AACAGGGCAG GCGAATGTTC 
CCCTTTCTGA GTTTTAATAT CACTGGACTG AACCTGACTG CGCATTATAA TGTGTTTGTG 
GAGATTGTTC TGGCCGATCC GAACCACTGG AGATTTCAGG GCGGAAAGTG GGTGACCTGC 
GGGAAAGCGG ACAACAACAT GCAAGGAAAC AAGGTGTATG TTCACCCAGA GTCACCAAAC 
ACTGGAGCGC ACTGGATGAG ACAAGAAATA TCTTTCGGAA AACTGAAGCT AACCAACAAC 
AAAGGAGCAA ATAACAACAA TACTCAGATG ATTGTCCTGC AGTCCCTGCA TAAGTACCAG 
CCTCGGCTTC ACATTGTGGA GGTGACTGAG GACGGTGTGG AGGACATGAG CAGCGAAGCT 
AAAACACAGA CCTTCACTTT CCCTGAAAAC CAGTTCATCG CTGTAACGGC TTACCAAAAC 
ACAGACATCA CACAGCTCAA GATCGACCAC AACCCCTTTG CAAAAGGCTT CAGGGACAAT 
TATGATTCGA TGTACACAGC TCCAGAGAGC GACAGACTGA CGCCCTCTCC TACTGACTCT 
CCGCGGTCGC ACCAGATTGT CCCCGGCGCC CGCTACGCAA TGCAGCCCTT CTTCCAAGAC 
CAATTTGTCA ACAACCTTCC CCAAAACCAC CGATTCTACG GCAGCGAGCG CGCCGTTCCC 
CAAACCAACG GCCTGCTGTC TCCTCAGAGC GAAGATTCGG CCGCGGGCTC GGCGTCAGCT 
CAGCGCTGGT TTGTGGGGCC GGTGCAACAA AGTGGTGCGA CCACCAAACT GGATCTGTCC 
TATGACAGTG AGTACTCTGC CTCCAGTCTG CTTCCCTACG GCATCAAACC ACTGCCGCTC 
CAGAGTCCAC ATGCACTCGG CTACTATCCT GATTCTGCAT TCGCATCTAT GGCAGCCGGA 
TGGAGCAGCA GAAGTTCCTA CCAGAGAAAA ATGACCACCG GCCTGCCGTG GTCACCCCGA 
CCAAGCCCTC CGGCATACAC CGATGACCTG CTGTCCTCCA AAGACAAGCT GCAGGATGAA 
AACTCCACAG CGGCCCCTAC GGCTGCCAGC ACAGGCCCCT GGATGGAGGC ACCTCCGGTG 
CTGAAAAGCG TGGATTCAGG CGACACCAGC GGTTACTCCA TGGCCTGTAA GCGGCGGCGT 
GTTTCGCCTG GAGGCTCCAG TACAGACGCT TCACCCACTA TAAAGTGTGA GGACTTGAGC 
TCTGAGGAGT ACAACAAAGA GAGCCATAAA GCCATGGGTT ATTACGCCTT CTACACCAGC 
CCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005159376.1
CDS	492..2477
Translation	MQLESILPGASVNLPKTFYNLSSSSESTNNSPGSTQIDFQDMDRTDSEQSSGAKKFLMDDADSESFTGTKAAAAATAAPDARKSSPVIGADDELSSTRRYNIDDLGTDRYFISSSQPSSDVANPCSLFPYGGQTGSVYSGSNGSRYSSSLHYGSVLPPAGFSSAVCASRSQFGGGYQFGQGPGCLYPSYPGPGSSLSSMPIPGSGSAARAQVYLCNRPLWLKFHRHQTEMIITKQGRRMFPFLSFNITGLNLTAHYNVFVEIVLADPNHWRFQGGKWVTCGKADNNMQGNKVYVHPESPNTGAHWMRQEISFGKLKLTNNKGANNNNTQMIVLQSLHKYQPRLHIVEVTEDGVEDMSSEAKTQTFTFPENQFIAVTAYQNTDITQLKIDHNPFAKGFRDNYDSMYTAPESDRLTPSPTDSPRSHQIVPGARYAMQPFFQDQFVNNLPQNHRFYGSERAVPQTNGLLSPQSEDSAAGSASAQRWFVGPVQQSGATTKLDLSYDSEYSASSLLPYGIKPLPLQSPHALGYYPDSAFASMAAGWSSRSSYQRKMTTGLPWSPRPSPPAYTDDLLSSKDKLQDENSTAAPTAASTGPWMEAPPVLKSVDSGDTSGYSMACKRRRVSPGGSSTDASPTIKCEDLSSEEYNKESHKAMGYYAFYTSP

Target ORF information:

RefSeq Version	XM_005159319.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio eomesodermin homolog a (eomesa), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005159319.3

ORF Insert Sequence:

ATGCAGTTAG AAAGCATCCT CCCTGGCGCA TCTGTAAACT TACCCAAGAC CTTCTACAAC 
CTGTCCTCAT CCTCCGAAAG CACCAATAAT AGCCCGGGGT CAACGCAGAT CGACTTTCAG 
GACATGGACC GGACGGATTC GGAGCAGAGT TCTGGTGCCA AGAAGTTTCT GATGGATGAT 
GCTGACAGTG AGAGCTTTAC CGGGACTAAA GCAGCAGCAG CAGCCACCGC CGCACCGGAC 
GCGCGTAAAA GTTCTCCGGT GATCGGCGCG GATGATGAGC TCTCCAGCAC CCGCAGATAC 
AACATCGATG ATCTGGGCAC TGATCGATAC TTCATATCGT CCTCTCAACC GAGTTCTGAC 
GTGGCCAACC CGTGCTCTCT GTTCCCGTAC GGAGGGCAGA CCGGGTCGGT GTACAGCGGC 
TCGAATGGCT CCCGGTATTC GTCGTCTCTG CATTACGGTT CGGTTCTTCC ACCCGCCGGG 
TTCTCATCCG CCGTGTGCGC CAGCAGGAGT CAGTTTGGGG GTGGTTATCA GTTCGGACAG 
GGTCCCGGGT GTCTGTACCC GTCCTATCCC GGTCCAGGCT CCAGTCTCAG CTCGATGCCC 
ATTCCAGGTT CGGGCTCCGC GGCGCGGGCG CAGGTTTACC TGTGCAACAG ACCGCTGTGG 
CTCAAATTTC ACCGCCATCA AACTGAGATG ATCATAACTA AACAGGGCAG GCGAATGTTC 
CCCTTTCTGA GTTTTAATAT CACTGGACTG AACCTGACTG CGCATTATAA TGTGTTTGTG 
GAGATTGTTC TGGCCGATCC GAACCACTGG AGATTTCAGG GCGGAAAGTG GGTGACCTGC 
GGGAAAGCGG ACAACAACAT GCAAGGAAAC AAGGTGTATG TTCACCCAGA GTCACCAAAC 
ACTGGAGCGC ACTGGATGAG ACAAGAAATA TCTTTCGGAA AACTGAAGCT AACCAACAAC 
AAAGGAGCAA ATAACAACAA TACTCAGATG ATTGTCCTGC AGTCCCTGCA TAAGTACCAG 
CCTCGGCTTC ACATTGTGGA GGTGACTGAG GACGGTGTGG AGGACATGAG CAGCGAAGCT 
AAAACACAGA CCTTCACTTT CCCTGAAAAC CAGTTCATCG CTGTAACGGC TTACCAAAAC 
ACAGACATCA CACAGCTCAA GATCGACCAC AACCCCTTTG CAAAAGGCTT CAGGGACAAT 
TATGATTCGA TGTACACAGC TCCAGAGAGC GACAGACTGA CGCCCTCTCC TACTGACTCT 
CCGCGGTCGC ACCAGATTGT CCCCGGCGCC CGCTACGCAA TGCAGCCCTT CTTCCAAGAC 
CAATTTGTCA ACAACCTTCC CCAAAACCAC CGATTCTACG GCAGCGAGCG CGCCGTTCCC 
CAAACCAACG GCCTGCTGTC TCCTCAGAGC GAAGATTCGG CCGCGGGCTC GGCGTCAGCT 
CAGCGCTGGT TTGTGGGGCC GGTGCAACAA AGTGGTGCGA CCACCAAACT GGATCTGTCC 
TATGACAGTG AGTACTCTGC CTCCAGTCTG CTTCCCTACG GCATCAAACC ACTGCCGCTC 
CAGAGTCCAC ATGCACTCGG CTACTATCCT GATTCTGCAT TCGCATCTAT GGCAGCCGGA 
TGGAGCAGCA GAAGTTCCTA CCAGAGAAAA ATGACCACCG GCCTGCCGTG GTCACCCCGA 
CCAAGCCCTC CGGCATACAC CGATGACCTG CTGTCCTCCA AAGACAAGCT GCAGGATGAA 
AACTCCACAG CGGCCCCTAC GGCTGCCAGC ACAGGCCCCT GGATGGAGGC ACCTCCGGTG 
CTGAAAAGCG TGGATTCAGG CGACACCAGC GGTTACTCCA TGGCCTGTAA GCGGCGGCGT 
GTTTCGCCTG GAGGCTCCAG TACAGACGCT TCACCCACTA TAAAGTGTGA GGACTTGAGC 
TCTGAGGAGT ACAACAAAGA GAGCCATAAA GCCATGGGTT ATTACGCCTT CTACACCAGC 
CCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

eomesa ( XM_005159319.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005159376.1 Danio rerio eomesodermin homolog a (eomesa), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa23698
Clone ID Related Accession (Same CDS sequence)	XM_005159321.3 , XM_005159319.3 , XM_005159319.4 , XM_005159321.4
Accession Version	XM_005159319.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1986bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	eomesodermin homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007130.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005159319.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGTTAG AAAGCATCCT CCCTGGCGCA TCTGTAAACT TACCCAAGAC CTTCTACAAC 
CTGTCCTCAT CCTCCGAAAG CACCAATAAT AGCCCGGGGT CAACGCAGAT CGACTTTCAG 
GACATGGACC GGACGGATTC GGAGCAGAGT TCTGGTGCCA AGAAGTTTCT GATGGATGAT 
GCTGACAGTG AGAGCTTTAC CGGGACTAAA GCAGCAGCAG CAGCCACCGC CGCACCGGAC 
GCGCGTAAAA GTTCTCCGGT GATCGGCGCG GATGATGAGC TCTCCAGCAC CCGCAGATAC 
AACATCGATG ATCTGGGCAC TGATCGATAC TTCATATCGT CCTCTCAACC GAGTTCTGAC 
GTGGCCAACC CGTGCTCTCT GTTCCCGTAC GGAGGGCAGA CCGGGTCGGT GTACAGCGGC 
TCGAATGGCT CCCGGTATTC GTCGTCTCTG CATTACGGTT CGGTTCTTCC ACCCGCCGGG 
TTCTCATCCG CCGTGTGCGC CAGCAGGAGT CAGTTTGGGG GTGGTTATCA GTTCGGACAG 
GGTCCCGGGT GTCTGTACCC GTCCTATCCC GGTCCAGGCT CCAGTCTCAG CTCGATGCCC 
ATTCCAGGTT CGGGCTCCGC GGCGCGGGCG CAGGTTTACC TGTGCAACAG ACCGCTGTGG 
CTCAAATTTC ACCGCCATCA AACTGAGATG ATCATAACTA AACAGGGCAG GCGAATGTTC 
CCCTTTCTGA GTTTTAATAT CACTGGACTG AACCTGACTG CGCATTATAA TGTGTTTGTG 
GAGATTGTTC TGGCCGATCC GAACCACTGG AGATTTCAGG GCGGAAAGTG GGTGACCTGC 
GGGAAAGCGG ACAACAACAT GCAAGGAAAC AAGGTGTATG TTCACCCAGA GTCACCAAAC 
ACTGGAGCGC ACTGGATGAG ACAAGAAATA TCTTTCGGAA AACTGAAGCT AACCAACAAC 
AAAGGAGCAA ATAACAACAA TACTCAGATG ATTGTCCTGC AGTCCCTGCA TAAGTACCAG 
CCTCGGCTTC ACATTGTGGA GGTGACTGAG GACGGTGTGG AGGACATGAG CAGCGAAGCT 
AAAACACAGA CCTTCACTTT CCCTGAAAAC CAGTTCATCG CTGTAACGGC TTACCAAAAC 
ACAGACATCA CACAGCTCAA GATCGACCAC AACCCCTTTG CAAAAGGCTT CAGGGACAAT 
TATGATTCGA TGTACACAGC TCCAGAGAGC GACAGACTGA CGCCCTCTCC TACTGACTCT 
CCGCGGTCGC ACCAGATTGT CCCCGGCGCC CGCTACGCAA TGCAGCCCTT CTTCCAAGAC 
CAATTTGTCA ACAACCTTCC CCAAAACCAC CGATTCTACG GCAGCGAGCG CGCCGTTCCC 
CAAACCAACG GCCTGCTGTC TCCTCAGAGC GAAGATTCGG CCGCGGGCTC GGCGTCAGCT 
CAGCGCTGGT TTGTGGGGCC GGTGCAACAA AGTGGTGCGA CCACCAAACT GGATCTGTCC 
TATGACAGTG AGTACTCTGC CTCCAGTCTG CTTCCCTACG GCATCAAACC ACTGCCGCTC 
CAGAGTCCAC ATGCACTCGG CTACTATCCT GATTCTGCAT TCGCATCTAT GGCAGCCGGA 
TGGAGCAGCA GAAGTTCCTA CCAGAGAAAA ATGACCACCG GCCTGCCGTG GTCACCCCGA 
CCAAGCCCTC CGGCATACAC CGATGACCTG CTGTCCTCCA AAGACAAGCT GCAGGATGAA 
AACTCCACAG CGGCCCCTAC GGCTGCCAGC ACAGGCCCCT GGATGGAGGC ACCTCCGGTG 
CTGAAAAGCG TGGATTCAGG CGACACCAGC GGTTACTCCA TGGCCTGTAA GCGGCGGCGT 
GTTTCGCCTG GAGGCTCCAG TACAGACGCT TCACCCACTA TAAAGTGTGA GGACTTGAGC 
TCTGAGGAGT ACAACAAAGA GAGCCATAAA GCCATGGGTT ATTACGCCTT CTACACCAGC 
CCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005159376.1
CDS	581..2566
Translation	MQLESILPGASVNLPKTFYNLSSSSESTNNSPGSTQIDFQDMDRTDSEQSSGAKKFLMDDADSESFTGTKAAAAATAAPDARKSSPVIGADDELSSTRRYNIDDLGTDRYFISSSQPSSDVANPCSLFPYGGQTGSVYSGSNGSRYSSSLHYGSVLPPAGFSSAVCASRSQFGGGYQFGQGPGCLYPSYPGPGSSLSSMPIPGSGSAARAQVYLCNRPLWLKFHRHQTEMIITKQGRRMFPFLSFNITGLNLTAHYNVFVEIVLADPNHWRFQGGKWVTCGKADNNMQGNKVYVHPESPNTGAHWMRQEISFGKLKLTNNKGANNNNTQMIVLQSLHKYQPRLHIVEVTEDGVEDMSSEAKTQTFTFPENQFIAVTAYQNTDITQLKIDHNPFAKGFRDNYDSMYTAPESDRLTPSPTDSPRSHQIVPGARYAMQPFFQDQFVNNLPQNHRFYGSERAVPQTNGLLSPQSEDSAAGSASAQRWFVGPVQQSGATTKLDLSYDSEYSASSLLPYGIKPLPLQSPHALGYYPDSAFASMAAGWSSRSSYQRKMTTGLPWSPRPSPPAYTDDLLSSKDKLQDENSTAAPTAASTGPWMEAPPVLKSVDSGDTSGYSMACKRRRVSPGGSSTDASPTIKCEDLSSEEYNKESHKAMGYYAFYTSP

Target ORF information:

RefSeq Version	XM_005159319.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio eomesodermin homolog a (eomesa), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005159319.4

ORF Insert Sequence:

ATGCAGTTAG AAAGCATCCT CCCTGGCGCA TCTGTAAACT TACCCAAGAC CTTCTACAAC 
CTGTCCTCAT CCTCCGAAAG CACCAATAAT AGCCCGGGGT CAACGCAGAT CGACTTTCAG 
GACATGGACC GGACGGATTC GGAGCAGAGT TCTGGTGCCA AGAAGTTTCT GATGGATGAT 
GCTGACAGTG AGAGCTTTAC CGGGACTAAA GCAGCAGCAG CAGCCACCGC CGCACCGGAC 
GCGCGTAAAA GTTCTCCGGT GATCGGCGCG GATGATGAGC TCTCCAGCAC CCGCAGATAC 
AACATCGATG ATCTGGGCAC TGATCGATAC TTCATATCGT CCTCTCAACC GAGTTCTGAC 
GTGGCCAACC CGTGCTCTCT GTTCCCGTAC GGAGGGCAGA CCGGGTCGGT GTACAGCGGC 
TCGAATGGCT CCCGGTATTC GTCGTCTCTG CATTACGGTT CGGTTCTTCC ACCCGCCGGG 
TTCTCATCCG CCGTGTGCGC CAGCAGGAGT CAGTTTGGGG GTGGTTATCA GTTCGGACAG 
GGTCCCGGGT GTCTGTACCC GTCCTATCCC GGTCCAGGCT CCAGTCTCAG CTCGATGCCC 
ATTCCAGGTT CGGGCTCCGC GGCGCGGGCG CAGGTTTACC TGTGCAACAG ACCGCTGTGG 
CTCAAATTTC ACCGCCATCA AACTGAGATG ATCATAACTA AACAGGGCAG GCGAATGTTC 
CCCTTTCTGA GTTTTAATAT CACTGGACTG AACCTGACTG CGCATTATAA TGTGTTTGTG 
GAGATTGTTC TGGCCGATCC GAACCACTGG AGATTTCAGG GCGGAAAGTG GGTGACCTGC 
GGGAAAGCGG ACAACAACAT GCAAGGAAAC AAGGTGTATG TTCACCCAGA GTCACCAAAC 
ACTGGAGCGC ACTGGATGAG ACAAGAAATA TCTTTCGGAA AACTGAAGCT AACCAACAAC 
AAAGGAGCAA ATAACAACAA TACTCAGATG ATTGTCCTGC AGTCCCTGCA TAAGTACCAG 
CCTCGGCTTC ACATTGTGGA GGTGACTGAG GACGGTGTGG AGGACATGAG CAGCGAAGCT 
AAAACACAGA CCTTCACTTT CCCTGAAAAC CAGTTCATCG CTGTAACGGC TTACCAAAAC 
ACAGACATCA CACAGCTCAA GATCGACCAC AACCCCTTTG CAAAAGGCTT CAGGGACAAT 
TATGATTCGA TGTACACAGC TCCAGAGAGC GACAGACTGA CGCCCTCTCC TACTGACTCT 
CCGCGGTCGC ACCAGATTGT CCCCGGCGCC CGCTACGCAA TGCAGCCCTT CTTCCAAGAC 
CAATTTGTCA ACAACCTTCC CCAAAACCAC CGATTCTACG GCAGCGAGCG CGCCGTTCCC 
CAAACCAACG GCCTGCTGTC TCCTCAGAGC GAAGATTCGG CCGCGGGCTC GGCGTCAGCT 
CAGCGCTGGT TTGTGGGGCC GGTGCAACAA AGTGGTGCGA CCACCAAACT GGATCTGTCC 
TATGACAGTG AGTACTCTGC CTCCAGTCTG CTTCCCTACG GCATCAAACC ACTGCCGCTC 
CAGAGTCCAC ATGCACTCGG CTACTATCCT GATTCTGCAT TCGCATCTAT GGCAGCCGGA 
TGGAGCAGCA GAAGTTCCTA CCAGAGAAAA ATGACCACCG GCCTGCCGTG GTCACCCCGA 
CCAAGCCCTC CGGCATACAC CGATGACCTG CTGTCCTCCA AAGACAAGCT GCAGGATGAA 
AACTCCACAG CGGCCCCTAC GGCTGCCAGC ACAGGCCCCT GGATGGAGGC ACCTCCGGTG 
CTGAAAAGCG TGGATTCAGG CGACACCAGC GGTTACTCCA TGGCCTGTAA GCGGCGGCGT 
GTTTCGCCTG GAGGCTCCAG TACAGACGCT TCACCCACTA TAAAGTGTGA GGACTTGAGC 
TCTGAGGAGT ACAACAAAGA GAGCCATAAA GCCATGGGTT ATTACGCCTT CTACACCAGC 
CCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

eomesa ( XM_005159321.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005159378.1 Danio rerio eomesodermin homolog a (eomesa), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa23698
Clone ID Related Accession (Same CDS sequence)	XM_005159321.3 , XM_005159319.3 , XM_005159319.4 , XM_005159321.4
Accession Version	XM_005159321.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1986bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	eomesodermin homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007130.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005159321.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGTTAG AAAGCATCCT CCCTGGCGCA TCTGTAAACT TACCCAAGAC CTTCTACAAC 
CTGTCCTCAT CCTCCGAAAG CACCAATAAT AGCCCGGGGT CAACGCAGAT CGACTTTCAG 
GACATGGACC GGACGGATTC GGAGCAGAGT TCTGGTGCCA AGAAGTTTCT GATGGATGAT 
GCTGACAGTG AGAGCTTTAC CGGGACTAAA GCAGCAGCAG CAGCCACCGC CGCACCGGAC 
GCGCGTAAAA GTTCTCCGGT GATCGGCGCG GATGATGAGC TCTCCAGCAC CCGCAGATAC 
AACATCGATG ATCTGGGCAC TGATCGATAC TTCATATCGT CCTCTCAACC GAGTTCTGAC 
GTGGCCAACC CGTGCTCTCT GTTCCCGTAC GGAGGGCAGA CCGGGTCGGT GTACAGCGGC 
TCGAATGGCT CCCGGTATTC GTCGTCTCTG CATTACGGTT CGGTTCTTCC ACCCGCCGGG 
TTCTCATCCG CCGTGTGCGC CAGCAGGAGT CAGTTTGGGG GTGGTTATCA GTTCGGACAG 
GGTCCCGGGT GTCTGTACCC GTCCTATCCC GGTCCAGGCT CCAGTCTCAG CTCGATGCCC 
ATTCCAGGTT CGGGCTCCGC GGCGCGGGCG CAGGTTTACC TGTGCAACAG ACCGCTGTGG 
CTCAAATTTC ACCGCCATCA AACTGAGATG ATCATAACTA AACAGGGCAG GCGAATGTTC 
CCCTTTCTGA GTTTTAATAT CACTGGACTG AACCTGACTG CGCATTATAA TGTGTTTGTG 
GAGATTGTTC TGGCCGATCC GAACCACTGG AGATTTCAGG GCGGAAAGTG GGTGACCTGC 
GGGAAAGCGG ACAACAACAT GCAAGGAAAC AAGGTGTATG TTCACCCAGA GTCACCAAAC 
ACTGGAGCGC ACTGGATGAG ACAAGAAATA TCTTTCGGAA AACTGAAGCT AACCAACAAC 
AAAGGAGCAA ATAACAACAA TACTCAGATG ATTGTCCTGC AGTCCCTGCA TAAGTACCAG 
CCTCGGCTTC ACATTGTGGA GGTGACTGAG GACGGTGTGG AGGACATGAG CAGCGAAGCT 
AAAACACAGA CCTTCACTTT CCCTGAAAAC CAGTTCATCG CTGTAACGGC TTACCAAAAC 
ACAGACATCA CACAGCTCAA GATCGACCAC AACCCCTTTG CAAAAGGCTT CAGGGACAAT 
TATGATTCGA TGTACACAGC TCCAGAGAGC GACAGACTGA CGCCCTCTCC TACTGACTCT 
CCGCGGTCGC ACCAGATTGT CCCCGGCGCC CGCTACGCAA TGCAGCCCTT CTTCCAAGAC 
CAATTTGTCA ACAACCTTCC CCAAAACCAC CGATTCTACG GCAGCGAGCG CGCCGTTCCC 
CAAACCAACG GCCTGCTGTC TCCTCAGAGC GAAGATTCGG CCGCGGGCTC GGCGTCAGCT 
CAGCGCTGGT TTGTGGGGCC GGTGCAACAA AGTGGTGCGA CCACCAAACT GGATCTGTCC 
TATGACAGTG AGTACTCTGC CTCCAGTCTG CTTCCCTACG GCATCAAACC ACTGCCGCTC 
CAGAGTCCAC ATGCACTCGG CTACTATCCT GATTCTGCAT TCGCATCTAT GGCAGCCGGA 
TGGAGCAGCA GAAGTTCCTA CCAGAGAAAA ATGACCACCG GCCTGCCGTG GTCACCCCGA 
CCAAGCCCTC CGGCATACAC CGATGACCTG CTGTCCTCCA AAGACAAGCT GCAGGATGAA 
AACTCCACAG CGGCCCCTAC GGCTGCCAGC ACAGGCCCCT GGATGGAGGC ACCTCCGGTG 
CTGAAAAGCG TGGATTCAGG CGACACCAGC GGTTACTCCA TGGCCTGTAA GCGGCGGCGT 
GTTTCGCCTG GAGGCTCCAG TACAGACGCT TCACCCACTA TAAAGTGTGA GGACTTGAGC 
TCTGAGGAGT ACAACAAAGA GAGCCATAAA GCCATGGGTT ATTACGCCTT CTACACCAGC 
CCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005159378.1
CDS	467..2452
Translation	MQLESILPGASVNLPKTFYNLSSSSESTNNSPGSTQIDFQDMDRTDSEQSSGAKKFLMDDADSESFTGTKAAAAATAAPDARKSSPVIGADDELSSTRRYNIDDLGTDRYFISSSQPSSDVANPCSLFPYGGQTGSVYSGSNGSRYSSSLHYGSVLPPAGFSSAVCASRSQFGGGYQFGQGPGCLYPSYPGPGSSLSSMPIPGSGSAARAQVYLCNRPLWLKFHRHQTEMIITKQGRRMFPFLSFNITGLNLTAHYNVFVEIVLADPNHWRFQGGKWVTCGKADNNMQGNKVYVHPESPNTGAHWMRQEISFGKLKLTNNKGANNNNTQMIVLQSLHKYQPRLHIVEVTEDGVEDMSSEAKTQTFTFPENQFIAVTAYQNTDITQLKIDHNPFAKGFRDNYDSMYTAPESDRLTPSPTDSPRSHQIVPGARYAMQPFFQDQFVNNLPQNHRFYGSERAVPQTNGLLSPQSEDSAAGSASAQRWFVGPVQQSGATTKLDLSYDSEYSASSLLPYGIKPLPLQSPHALGYYPDSAFASMAAGWSSRSSYQRKMTTGLPWSPRPSPPAYTDDLLSSKDKLQDENSTAAPTAASTGPWMEAPPVLKSVDSGDTSGYSMACKRRRVSPGGSSTDASPTIKCEDLSSEEYNKESHKAMGYYAFYTSP

Target ORF information:

RefSeq Version	XM_005159321.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio eomesodermin homolog a (eomesa), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005159321.4

ORF Insert Sequence:

ATGCAGTTAG AAAGCATCCT CCCTGGCGCA TCTGTAAACT TACCCAAGAC CTTCTACAAC 
CTGTCCTCAT CCTCCGAAAG CACCAATAAT AGCCCGGGGT CAACGCAGAT CGACTTTCAG 
GACATGGACC GGACGGATTC GGAGCAGAGT TCTGGTGCCA AGAAGTTTCT GATGGATGAT 
GCTGACAGTG AGAGCTTTAC CGGGACTAAA GCAGCAGCAG CAGCCACCGC CGCACCGGAC 
GCGCGTAAAA GTTCTCCGGT GATCGGCGCG GATGATGAGC TCTCCAGCAC CCGCAGATAC 
AACATCGATG ATCTGGGCAC TGATCGATAC TTCATATCGT CCTCTCAACC GAGTTCTGAC 
GTGGCCAACC CGTGCTCTCT GTTCCCGTAC GGAGGGCAGA CCGGGTCGGT GTACAGCGGC 
TCGAATGGCT CCCGGTATTC GTCGTCTCTG CATTACGGTT CGGTTCTTCC ACCCGCCGGG 
TTCTCATCCG CCGTGTGCGC CAGCAGGAGT CAGTTTGGGG GTGGTTATCA GTTCGGACAG 
GGTCCCGGGT GTCTGTACCC GTCCTATCCC GGTCCAGGCT CCAGTCTCAG CTCGATGCCC 
ATTCCAGGTT CGGGCTCCGC GGCGCGGGCG CAGGTTTACC TGTGCAACAG ACCGCTGTGG 
CTCAAATTTC ACCGCCATCA AACTGAGATG ATCATAACTA AACAGGGCAG GCGAATGTTC 
CCCTTTCTGA GTTTTAATAT CACTGGACTG AACCTGACTG CGCATTATAA TGTGTTTGTG 
GAGATTGTTC TGGCCGATCC GAACCACTGG AGATTTCAGG GCGGAAAGTG GGTGACCTGC 
GGGAAAGCGG ACAACAACAT GCAAGGAAAC AAGGTGTATG TTCACCCAGA GTCACCAAAC 
ACTGGAGCGC ACTGGATGAG ACAAGAAATA TCTTTCGGAA AACTGAAGCT AACCAACAAC 
AAAGGAGCAA ATAACAACAA TACTCAGATG ATTGTCCTGC AGTCCCTGCA TAAGTACCAG 
CCTCGGCTTC ACATTGTGGA GGTGACTGAG GACGGTGTGG AGGACATGAG CAGCGAAGCT 
AAAACACAGA CCTTCACTTT CCCTGAAAAC CAGTTCATCG CTGTAACGGC TTACCAAAAC 
ACAGACATCA CACAGCTCAA GATCGACCAC AACCCCTTTG CAAAAGGCTT CAGGGACAAT 
TATGATTCGA TGTACACAGC TCCAGAGAGC GACAGACTGA CGCCCTCTCC TACTGACTCT 
CCGCGGTCGC ACCAGATTGT CCCCGGCGCC CGCTACGCAA TGCAGCCCTT CTTCCAAGAC 
CAATTTGTCA ACAACCTTCC CCAAAACCAC CGATTCTACG GCAGCGAGCG CGCCGTTCCC 
CAAACCAACG GCCTGCTGTC TCCTCAGAGC GAAGATTCGG CCGCGGGCTC GGCGTCAGCT 
CAGCGCTGGT TTGTGGGGCC GGTGCAACAA AGTGGTGCGA CCACCAAACT GGATCTGTCC 
TATGACAGTG AGTACTCTGC CTCCAGTCTG CTTCCCTACG GCATCAAACC ACTGCCGCTC 
CAGAGTCCAC ATGCACTCGG CTACTATCCT GATTCTGCAT TCGCATCTAT GGCAGCCGGA 
TGGAGCAGCA GAAGTTCCTA CCAGAGAAAA ATGACCACCG GCCTGCCGTG GTCACCCCGA 
CCAAGCCCTC CGGCATACAC CGATGACCTG CTGTCCTCCA AAGACAAGCT GCAGGATGAA 
AACTCCACAG CGGCCCCTAC GGCTGCCAGC ACAGGCCCCT GGATGGAGGC ACCTCCGGTG 
CTGAAAAGCG TGGATTCAGG CGACACCAGC GGTTACTCCA TGGCCTGTAA GCGGCGGCGT 
GTTTCGCCTG GAGGCTCCAG TACAGACGCT TCACCCACTA TAAAGTGTGA GGACTTGAGC 
TCTGAGGAGT ACAACAAAGA GAGCCATAAA GCCATGGGTT ATTACGCCTT CTACACCAGC 
CCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Maternal Nanog is required for zebrafish embryo architecture and for cell viability during gastrulation.
Development (Cambridge, England)145(1)(2018 Jan)
Veil M,Schaechtle MA,Gao M,Kirner V,Buryanova L,Grethen R,Onichtchouk D

Zebrafish Regulatory T Cells Mediate Organ-Specific Regenerative Programs.
Developmental cell43(6)659-672.e5(2017 Dec)
Hui SP,Sheng DZ,Sugimoto K,Gonzalez-Rajal A,Nakagawa S,Hesselson D,Kikuchi K

Zebrafish FOXP3 is required for the maintenance of immune tolerance.
Developmental and comparative immunology73156-162(2017 Aug)
Sugimoto K,Hui SP,Sheng DZ,Nakayama M,Kikuchi K

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

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eomesa cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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