actn2b cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	actn2b
Entrez Gene ID	641575
Full Name	actinin, alpha 2b
Synonyms	actn2,wu:fd44c03,zgc:123223
General protein information	Preferred Names actinin, alpha 2b Names alpha-actinin-2 actinin, alpha 2
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001037573.1	NP_001032662.1	alpha-actinin-2

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	ACTN2	XP_001158729.2
	ACTN2	XP_001097795.1
Rattus norvegicus (Norway rat)	Actn2	NP_001163796.1
Gallus gallus (chicken)	ACTN2	NP_990654.1
Danio rerio (zebrafish)	actn2	NP_001032662.1
Xenopus tropicalis (tropical clawed frog)	actn2	NP_001005053.1
Homo sapiens (human)	ACTN2	NP_001094.1
Canis lupus familiaris (dog)	ACTN2	XP_856116.1
Bos taurus (cattle)	ACTN2	NP_001029807.1
Mus musculus (house mouse)	Actn2	NP_150371.4

Gene Ontology
Bibliography

Related articles in PubMed

In vitro characterization of zebrafish (Danio rerio) organic anion transporters Oat2a-e.
Dragojevi? J, Mihaljevi? I, Popovi? M, Zaja R, Smital T
Toxicology in vitro : an international journal published in association with BIBRA46246-256(2018 Feb)

Fipronil-induced enantioselective developmental toxicity to zebrafish embryo-larvae involves changes in DNA methylation.
Qian Y, Wang C, Wang J, Zhang X, Zhou Z, Zhao M, Lu C
Scientific reports7(1)2284(2017 May)

Evolution of complexity in the zebrafish synapse proteome.
Bay?s ?, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 Mar)

Establishment and growth responses of Nile tilapia embryonic stem-like cell lines under feeder-free condition.
Fan Z, Liu L, Huang X, Zhao Y, Zhou L, Wang D, Wei J
Development, growth & differentiation59(2)83-93(2017 Feb)

Decellularized zebrafish cardiac extracellular matrix induces mammalian heart regeneration.
Chen WC, Wang Z, Missinato MA, Park DW, Long DW, Liu HJ, Zeng X, Yates NA, Kim K, Wang Y
Science advances2(11)e1600844(2016 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

alpha-Actinin2 is required for the lateral alignment of Z discs and ventricular chamber enlargement during zebrafish cardiogenesis.
Title: ?-Actinin2 is required for the lateral alignment of Z discs and ventricular chamber enlargement during zebrafish cardiogenesis.

The following actn2b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the actn2b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa35991	NM_001037573.1 Latest version!	Danio rerio actinin, alpha 2b (actn2b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

actn2b ( NM_001037573.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001032662.1 Danio rerio actinin, alpha 2b (actn2b), mRNA.

Price & Availability↑

CloneID	ODa35991
Clone ID Related Accession (Same CDS sequence)	NM_001037573.1
Accession Version	NM_001037573.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2688bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-26
Organism	Danio rerio(zebrafish)
Product	alpha-actinin-2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC110103.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC110103.1, FJ979800.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGATGAATC AGATCGAGCT TTCAGTGCCT TATGATAACG GATATGAAAT AGACGAGTAC 
ATGATGCAGG AGGATGAATG GGACCGGGAT TTACTGCTGG ATCCAGCATG GGAAAAGCAA 
CAAAGGAAGA CGTTCACTGC CTGGTGTAAC TCTCGCCTGA GAAAAGCTGG GACGCAGATC 
GAGAACATAG AGGAGGACTT TAGAAATGGC CTCAAACTAA TGCTGCTGCT TGAAGTCATC 
TCAGGTGAGA GGTTACCCAA GCCTGACAGA GGGAAGATGC GTTTTCACAA GATAGCTAAT 
GTCAATAAAG CCCTGGATTT CATCACCAGT AAAGGTGTGA AACTGGTCTC CATTGGAGCT 
GAAGAAATTG TGGATGGTAA TGTGAAAATG ACTCTTGGAA TGATCTGGAC AATCATCCTT 
CGCTTCGCCA TTCAGGATAT CTCTGTTGAA GAAACTTCGG CCAAAGAAGG ACTTCTGCTG 
TGGTGTCAGA GAAAGACTGC CCCGTACCGA AATGTCAATG TTCAAAACTT CCATGTCAGC 
TGGAAGGATG GATTGGCGCT GTGCGCTCTC ATCCACAGGC ACAGGCCTGA CCTCATTGAC 
TACGCCAAGC TGAACAAGGA TGATCCACTG GGTAATTTAA ATCTGGCATT TGACATTGCT 
GAAAAACATC TGGACATCCC CAAAATGCTG GATGCAGAGG ACATCCTGAG CACCCCTAAA 
CCTGATGAGA GGGCTATTAT GACATACGTG TCCTGCTTTT ACCATGCATT TGCTGGAGCT 
GAGCAGGCTG AAACTGCTGC GAACAGGATC TGTAAGGTGC TTGGAGTGAA TCAGGAAAAT 
GAGAAATTGA TGGAGGATTA TGAAAGGCTG GCTAGTGAGC TGTTGGAGTG GATCAGACGT 
ACCATTCCTT GGCTGGAGAA TCGTGCAGCT GAGAAGACCA TGGTTGAGAT GCAGAGGAAG 
CTGGAAGATT TTCGAGATTA CCGCAGGATG CACAAACCAC CCAAAGTGCA GGAGAAATGT 
CAGCTGGAAA TCAGCTTCAA CACGCTGCAG ACCAAACTCC GCATCAGCAA CCGGCCTGCG 
TTTATGCCCT CTGAGGGCAA AATGGTGTCG GACATAGCGA GTGCGTGGCA AGGGCTGGAA 
CAAGCAGAGA AGGGCTATGA GGAATGGCTG CTGACTGAGA TCAGGAGGCT GGAAAGAGTG 
GAGCATCTAG CTGAGAAATT CAGACAGAAA GCTTCTAACC ATGAGTCCTG GGCTACAGGT 
AAGGAAGAGA TGTTGACCCG GAAGGACTAC GAGTCAGCGT CTCTAATGGA AGTGCGAGCT 
TTGCTGCGGA AACACGAAGC GTTTGAGAGT GACCTTTCTG CACACCAGGA CAGAGTTGAG 
CAGATCGCTG CTATTGCTCA GGAGCTCAAT GAGCTGGACT ATCATGATGT TGCATCCGTG 
AATCAAAGGT GTCAAAGTAT TTGTGACCTC TGGGACCATC TGGGCACGCT CACCCAGAGA 
CGCAGAGAGG CTTTGGAGAG GACAGAGAAA CTTTTGGAGA CGGTGGATCA GTTGTTTCTG 
GAATATGCAA AGAGATCTGC ACCCTTTAAT AACTGGATGG AAGGAGCTAT GGAAGACTTG 
CAGGACATGT TCATTGTGCA CACAATTGAG GAAGTCCAGA CTCTGATAGC TGCTCATGAG 
CAGTTTAAGG CCACCCTTTC TGAGGCAGAT GCAGAGAGAC AGGCGATCCT TGGCATTCAG 
CAGGAAGTCC TGAAGATCTT CCAGAGTTAT GGCATCCGTG GAGATCTTAC AAATCCCTAC 
AGCACTATTA CTGCAGAAGA AATTGCCATC AAATGGGACA AGGTGAAGAA GCTGGTGCCC 
CAGCGTGACA GTGCACTGCA GGAGGAGTTA GCGAGACAGC ATGCTAACGA AAGACTCAGA 
CGCCAGTTTG CTGCCCAAGC CAACCTCATC GGACCCTGGA TACAGACTAG GATGGAGGAA 
ATTGGTCATT GTTCTATGGC GATGGGCGGA ACTCTGGAGG ATCAGATGAC CCAACTGAAG 
CAGTATGAGC ATGTGATCGT CAGCTACAAA TCCAACATAG ACCGACTTGA GGGAGATCAC 
CAACTCATTC AGGAGTCACT TATCTTTGAC AACAAACACA CTAACTACAC TATGGAGCAT 
GTTCGCGTTG GCTGGGAGCT CCTATTGACC ACGATTGCTC GCACAATTAA TGAAATCGAG 
ACTCAAATTT TGACCCGTGA TGCAAAAGGC ATCAGCCAGG AGCAGATGCA TGAGTTCCGG 
TCCTCTTTCA ACCATTTTGA TCGGAAGGAG AACGGGGGCA TGGATTCTGA TGACTTTAGG 
GCCTGTCTGA TCTCGATGGG TTATGATCTG GGAGAGGTGG AGTTTGCTCG GATCATGATG 
CTGGTGGATC CAAATGCTTC AGGCAATGTG TCGTTCCAGT CCTTCATTGA TTTCATGACT 
AGAGAGACAG CAGACACAGA CACAGCTGAA CAGGTCATTG CTTCCTTCAA GATCCTCGCT 
GCAGACAAGC CGTATATTCT GTCAGATGAG TTGAGGCGAG AGCTTCCTCC AGACCAGGCC 
GAGTACTGTA TCAGTCGAAT GCCTCCTTAC TGTGGCCCCG GGGCCCTGCC TGGAGCCTTG 
GACTACACCG CCTTCTCCAC CGCCCTATAT GGAGAAAGTG ACCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001032662.1
CDS	164..2851
Translation	MMNQIELSVPYDNGYEIDEYMMQEDEWDRDLLLDPAWEKQQRKTFTAWCNSRLRKAGTQIENIEEDFRNGLKLMLLLEVISGERLPKPDRGKMRFHKIANVNKALDFITSKGVKLVSIGAEEIVDGNVKMTLGMIWTIILRFAIQDISVEETSAKEGLLLWCQRKTAPYRNVNVQNFHVSWKDGLALCALIHRHRPDLIDYAKLNKDDPLGNLNLAFDIAEKHLDIPKMLDAEDILSTPKPDERAIMTYVSCFYHAFAGAEQAETAANRICKVLGVNQENEKLMEDYERLASELLEWIRRTIPWLENRAAEKTMVEMQRKLEDFRDYRRMHKPPKVQEKCQLEISFNTLQTKLRISNRPAFMPSEGKMVSDIASAWQGLEQAEKGYEEWLLTEIRRLERVEHLAEKFRQKASNHESWATGKEEMLTRKDYESASLMEVRALLRKHEAFESDLSAHQDRVEQIAAIAQELNELDYHDVASVNQRCQSICDLWDHLGTLTQRRREALERTEKLLETVDQLFLEYAKRSAPFNNWMEGAMEDLQDMFIVHTIEEVQTLIAAHEQFKATLSEADAERQAILGIQQEVLKIFQSYGIRGDLTNPYSTITAEEIAIKWDKVKKLVPQRDSALQEELARQHANERLRRQFAAQANLIGPWIQTRMEEIGHCSMAMGGTLEDQMTQLKQYEHVIVSYKSNIDRLEGDHQLIQESLIFDNKHTNYTMEHVRVGWELLLTTIARTINEIETQILTRDAKGISQEQMHEFRSSFNHFDRKENGGMDSDDFRACLISMGYDLGEVEFARIMMLVDPNASGNVSFQSFIDFMTRETADTDTAEQVIASFKILAADKPYILSDELRRELPPDQAEYCISRMPPYCGPGALPGALDYTAFSTALYGESDL

Target ORF information:

RefSeq Version	NM_001037573.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio actinin, alpha 2b (actn2b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001037573.1

ORF Insert Sequence:

ATGATGAATC AGATCGAGCT TTCAGTGCCT TATGATAACG GATATGAAAT AGACGAGTAC 
ATGATGCAGG AGGATGAATG GGACCGGGAT TTACTGCTGG ATCCAGCATG GGAAAAGCAA 
CAAAGGAAGA CGTTCACTGC CTGGTGTAAC TCTCGCCTGA GAAAAGCTGG GACGCAGATC 
GAGAACATAG AGGAGGACTT TAGAAATGGC CTCAAACTAA TGCTGCTGCT TGAAGTCATC 
TCAGGTGAGA GGTTACCCAA GCCTGACAGA GGGAAGATGC GTTTTCACAA GATAGCTAAT 
GTCAATAAAG CCCTGGATTT CATCACCAGT AAAGGTGTGA AACTGGTCTC CATTGGAGCT 
GAAGAAATTG TGGATGGTAA TGTGAAAATG ACTCTTGGAA TGATCTGGAC AATCATCCTT 
CGCTTCGCCA TTCAGGATAT CTCTGTTGAA GAAACTTCGG CCAAAGAAGG ACTTCTGCTG 
TGGTGTCAGA GAAAGACTGC CCCGTACCGA AATGTCAATG TTCAAAACTT CCATGTCAGC 
TGGAAGGATG GATTGGCGCT GTGCGCTCTC ATCCACAGGC ACAGGCCTGA CCTCATTGAC 
TACGCCAAGC TGAACAAGGA TGATCCACTG GGTAATTTAA ATCTGGCATT TGACATTGCT 
GAAAAACATC TGGACATCCC CAAAATGCTG GATGCAGAGG ACATCCTGAG CACCCCTAAA 
CCTGATGAGA GGGCTATTAT GACATACGTG TCCTGCTTTT ACCATGCATT TGCTGGAGCT 
GAGCAGGCTG AAACTGCTGC GAACAGGATC TGTAAGGTGC TTGGAGTGAA TCAGGAAAAT 
GAGAAATTGA TGGAGGATTA TGAAAGGCTG GCTAGTGAGC TGTTGGAGTG GATCAGACGT 
ACCATTCCTT GGCTGGAGAA TCGTGCAGCT GAGAAGACCA TGGTTGAGAT GCAGAGGAAG 
CTGGAAGATT TTCGAGATTA CCGCAGGATG CACAAACCAC CCAAAGTGCA GGAGAAATGT 
CAGCTGGAAA TCAGCTTCAA CACGCTGCAG ACCAAACTCC GCATCAGCAA CCGGCCTGCG 
TTTATGCCCT CTGAGGGCAA AATGGTGTCG GACATAGCGA GTGCGTGGCA AGGGCTGGAA 
CAAGCAGAGA AGGGCTATGA GGAATGGCTG CTGACTGAGA TCAGGAGGCT GGAAAGAGTG 
GAGCATCTAG CTGAGAAATT CAGACAGAAA GCTTCTAACC ATGAGTCCTG GGCTACAGGT 
AAGGAAGAGA TGTTGACCCG GAAGGACTAC GAGTCAGCGT CTCTAATGGA AGTGCGAGCT 
TTGCTGCGGA AACACGAAGC GTTTGAGAGT GACCTTTCTG CACACCAGGA CAGAGTTGAG 
CAGATCGCTG CTATTGCTCA GGAGCTCAAT GAGCTGGACT ATCATGATGT TGCATCCGTG 
AATCAAAGGT GTCAAAGTAT TTGTGACCTC TGGGACCATC TGGGCACGCT CACCCAGAGA 
CGCAGAGAGG CTTTGGAGAG GACAGAGAAA CTTTTGGAGA CGGTGGATCA GTTGTTTCTG 
GAATATGCAA AGAGATCTGC ACCCTTTAAT AACTGGATGG AAGGAGCTAT GGAAGACTTG 
CAGGACATGT TCATTGTGCA CACAATTGAG GAAGTCCAGA CTCTGATAGC TGCTCATGAG 
CAGTTTAAGG CCACCCTTTC TGAGGCAGAT GCAGAGAGAC AGGCGATCCT TGGCATTCAG 
CAGGAAGTCC TGAAGATCTT CCAGAGTTAT GGCATCCGTG GAGATCTTAC AAATCCCTAC 
AGCACTATTA CTGCAGAAGA AATTGCCATC AAATGGGACA AGGTGAAGAA GCTGGTGCCC 
CAGCGTGACA GTGCACTGCA GGAGGAGTTA GCGAGACAGC ATGCTAACGA AAGACTCAGA 
CGCCAGTTTG CTGCCCAAGC CAACCTCATC GGACCCTGGA TACAGACTAG GATGGAGGAA 
ATTGGTCATT GTTCTATGGC GATGGGCGGA ACTCTGGAGG ATCAGATGAC CCAACTGAAG 
CAGTATGAGC ATGTGATCGT CAGCTACAAA TCCAACATAG ACCGACTTGA GGGAGATCAC 
CAACTCATTC AGGAGTCACT TATCTTTGAC AACAAACACA CTAACTACAC TATGGAGCAT 
GTTCGCGTTG GCTGGGAGCT CCTATTGACC ACGATTGCTC GCACAATTAA TGAAATCGAG 
ACTCAAATTT TGACCCGTGA TGCAAAAGGC ATCAGCCAGG AGCAGATGCA TGAGTTCCGG 
TCCTCTTTCA ACCATTTTGA TCGGAAGGAG AACGGGGGCA TGGATTCTGA TGACTTTAGG 
GCCTGTCTGA TCTCGATGGG TTATGATCTG GGAGAGGTGG AGTTTGCTCG GATCATGATG 
CTGGTGGATC CAAATGCTTC AGGCAATGTG TCGTTCCAGT CCTTCATTGA TTTCATGACT 
AGAGAGACAG CAGACACAGA CACAGCTGAA CAGGTCATTG CTTCCTTCAA GATCCTCGCT 
GCAGACAAGC CGTATATTCT GTCAGATGAG TTGAGGCGAG AGCTTCCTCC AGACCAGGCC 
GAGTACTGTA TCAGTCGAAT GCCTCCTTAC TGTGGCCCCG GGGCCCTGCC TGGAGCCTTG 
GACTACACCG CCTTCTCCAC CGCCCTATAT GGAGAAAGTG ACCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

In vitro characterization of zebrafish (Danio rerio) organic anion transporters Oat2a-e.
Toxicology in vitro : an international journal published in association with BIBRA46246-256(2018 Feb)
Dragojevi? J,Mihaljevi? I,Popovi? M,Zaja R,Smital T

Fipronil-induced enantioselective developmental toxicity to zebrafish embryo-larvae involves changes in DNA methylation.
Scientific reports7(1)2284(2017 May)
Qian Y,Wang C,Wang J,Zhang X,Zhou Z,Zhao M,Lu C

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 Mar)
Bay?s ?,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

Establishment and growth responses of Nile tilapia embryonic stem-like cell lines under feeder-free condition.
Development, growth & differentiation59(2)83-93(2017 Feb)
Fan Z,Liu L,Huang X,Zhao Y,Zhou L,Wang D,Wei J

Decellularized zebrafish cardiac extracellular matrix induces mammalian heart regeneration.
Science advances2(11)e1600844(2016 Nov)
Chen WC,Wang Z,Missinato MA,Park DW,Long DW,Liu HJ,Zeng X,Yates NA,Kim K,Wang Y