disp1 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	disp1
Entrez Gene ID	619201
Full Name	dispatched homolog 1 (Drosophila)
Synonyms	con,zgc:111866
General protein information	Preferred Names dispatched homolog 1 (Drosophila) Names protein dispatched homolog 1 chameleon dispatched 1 helix hlx protein chameleon
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_212800.1	NP_997965.1	protein dispatched homolog 1
XM_017352523.1	XP_017208012.1	protein dispatched homolog 1 isoform X1
XM_017352524.1	XP_017208013.1	protein dispatched homolog 1 isoform X1
XM_017352523.2	XP_017208012.1	protein dispatched homolog 1 isoform X1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	DISP1	XP_005640975.1
Gallus gallus (chicken)	DISP1	XP_004935361.1
Mus musculus (house mouse)	Disp1	NP_081142.3
Rattus norvegicus (Norway rat)	Disp1	NP_001099453.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004140	XP_313021.5
Homo sapiens (human)	DISP1	NP_116279.2
Pan troglodytes (chimpanzee)	DISP1	XP_001173201.2
	DISP1	XP_001099904.1
Danio rerio (zebrafish)	disp1	NP_997965.1
Caenorhabditis elegans (roundworm)	ptd-2	NP_505396.2
Xenopus tropicalis (tropical clawed frog)	disp1	XP_004914854.1
Drosophila melanogaster (fruit fly)	disp	NP_524734.2

Gene Ontology
Bibliography

Related articles in PubMed

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Visualization of Gli activity in craniofacial tissues of hedgehog-pathway reporter transgenic zebrafish.
Schwend T, Loucks EJ, Ahlgren SC
PloS one5(12)e14396(2010 Dec)

Zebrafish con/disp1 reveals multiple spatiotemporal requirements for Hedgehog-signaling in craniofacial development.
Schwend T, Ahlgren SC
BMC developmental biology959(2009 Nov)

Germ cell migration in zebrafish is cyclopamine-sensitive but Smoothened-independent.
Mich JK, Blaser H, Thomas NA, Firestone AJ, Yelon D, Raz E, Chen JK
Developmental biology328(2)342-54(2009 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Study shows that disp1 is required for post-migratory cranial neural crest cells (CNCC) to become properly patterned within the first arch, while the gene is dispensable for CNCC condensation and patterning in more posterior arches.
Title: Zebrafish con/disp1 reveals multiple spatiotemporal requirements for Hedgehog-signaling in craniofacial development.

Disp1 activity is essential for the secretion of lipid-modified Hh proteins from midline structures.
Title: Inactivation of dispatched 1 by the chameleon mutation disrupts Hedgehog signalling in the zebrafish embryo.

The following disp1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the disp1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa56588	XM_017352523.2 Latest version!	Danio rerio dispatched homolog 1 (Drosophila) (disp1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
ODa56588	XM_017352524.1 Latest version!	Danio rerio dispatched homolog 1 (Drosophila) (disp1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
ODa38265	NM_212800.1 Latest version!	Danio rerio dispatched homolog 1 (Drosophila) (disp1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
View Old Accession Versions >>
ODa56588	XM_017352523.1	Danio rerio dispatched homolog 1 (Drosophila) (disp1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

disp1 ( XM_017352523.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017208012.1 Danio rerio dispatched homolog 1 (Drosophila) (disp1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa56588
Clone ID Related Accession (Same CDS sequence)	XM_017352523.1 , XM_017352524.1 , XM_017352523.2
Accession Version	XM_017352523.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4395bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	protein dispatched homolog 1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCTGA GCGAGGACTT CACTGAGCTC CAGACCCTCA GCAATGGCAG ATCCTCCAGC 
ATTTTCCCCA CCACAAACAC ACCTGTAGAC CCGGAGGAGG AGGAGGAGCA GAGCGTTTCT 
GCATGCACAC AGCCCAATGA ACACACACAG ATGATGCAGA CAGGCCAGAA CGGCGGACTC 
AAAAGCACAC GTCTGTCTTC TTCCTCATCA TCATCATCAC AGCTCACAGA TGCTCAGCGA 
TTGGCTGTTC TGCTGCCCAA TGCTCTGCCT CCGGGTTTCT GTGTGTGCTG CTCGCTCTGT 
TGTAACGGGA ACCAGCAGGA TTGTGCTCTC TTCCAGAGCG CCGTGCCGTC CGCTGCCCTC 
CACATGGGCT CCTGCTGCGT CCAGGGCGTC CCCTGTTTCT GCATGCAGCA TCACTGGCCG 
GAGCATCTGC AGAACCAGCC CAACATGGCC ACGCTGAGTC CTCCGAGGCC TTTCCGGTTT 
CCTAAAAGTT ATGCAGAGCT GATTGCTGAT TGGCCGGTGG TGGTGTTGGG GATTTGCACT 
GTCCTCATTG TGGTTTGTGC TCTGGTCGGG ATCCTCGTTC CTGATCTGCC TGACTTCTCA 
AACCCACTGC AGGGGTTTGA GCCGAGAGGC ACTGCAATAG GACAGAGACT GGTTACGTGG 
AACAACATGG TGAAAAACAC CGGCTACAAG GCAACACTGG CAAACTATCC ATTCAAATAC 
GCAGACGAGC AGGCCAAAAG TCACCAAGAT GACCGGTGGT CCAAAGATCA TTACGATCGA 
AAGAAAAGAC AAGCGGAGTG GGACTTCAGC AAAGATAGTT TCTTCTGTGA TGTACCAGGT 
GACAGCTATT CCCGGATCGT ATTTGCTTCT GCTGAGGGCA AGAACTTGTG GAATATACAG 
GCCATTAAAT CCATGTGCAA TTTCGACAAT ACGCGGGTTC GGTCTCATCC CCAGTATCTG 
GATCTGTGTC AGCGCACCAC TGCTGCCTCC TGCTGTCCCA GCTGGACCCT CGGAAACTAC 
ATAGCCGTCC TTACAAACAA ATCTTCCTGC CAGAAGATCA CAGAGCGAGA CGTTTCTCAC 
ACCCTGAATA TCCTGCGCTC CTGTGCTAAA TTTTACCACA ACGGTACCCT CGGTCCTGAA 
TGCTGGGACA TGACCACCCG AAAAAAGGAC CTCAAGTGTG GCAACCTGCC GCGGAAGTGT 
ACAAAATACA ACGCTGTGTA TCAGATTTTC CACTTTTTGG TGGACAAAGA CTTTCTGAAT 
CCAAAGAACA CAGACTACCT CCCACCAAAC CTCAAATACA GCATGCTCTT TGCTCCTACA 
GAGAAAGGAG AGACCATGAT GAACATTTAC TTGGACAACT TTGAGAACCG TAACTCTTCG 
GATGGGATCA CGACTATAAC GGGAATAGAA TTTGGCATCA AACACAGTTT GTTTCAGGAC 
TACCTCCTGA CAGATACCAT GTATCCCGCA ATAGCTATTG TCATCGTCTT GGTGGTCATG 
TGCGTCTACA CGAGATCCAT GTTCATCACC CTTATGACGA TGTTTGCGAT CATCAGTTCG 
CTCATCGTTT CTTATTTCCT ATATCGAGTG GTCTTCAACT TTGATTTCTT CCCTTTCATG 
AACCTAACAG CGCTCATCAT CCTAGTAGGA ATTGGGGCTG ATGATGCATT TGTGCTCTGC 
GACGTCTGGA ATTACACAAA GTTTGACAAA CCTAATTCTG AGCTCTCTGA AACGGTGAGC 
ATCACACTTC AGCATGCAGC GCTTTCAATG TTTGTCACTA GCTTCACAAC GGCTGCTGCC 
TTCTATGCCA ACTATGTGAG CAACATCACT GCCATACGAT GTTTCGGTGT CTATGCTGGA 
ACCGCTATCT TGGTTAACTA CATACTGATG GTTACCTGGT TGCCAGCTGT GGTGGTCTTA 
CATGAGCGGT ACCTGGTTAA CCTTCTTACA TGTTCACGAA CACAAAACCA TCAATCGCAG 
GGCGCTGCAA CGTTTTGGAC AAGCCTGTGC CAGGTGGTCA ACAAGTGTCT TTTTAGCATC 
TCTGAAGCCT CGAGGATTTT CTTCGAGAAA GTTCTGCCTT GTGTCGTGAT CAAGTTGCGC 
TACTTGTGGC TCTTGTGGTT CCTGGCTTTG ACAGTAGGTG GCGCTTATGT GGTTTGCGTA 
AATCCCAAAA TGAAACTTCC CTCTTTGGAG CTCTCAGAAT TTCAAGTGTT TCGCTCCTCG 
CATCCCTTTG AACGCTATGA TGCGGAGTAC AAAAAACTCT TCATGTTTGA ACGGGTCAAC 
CATGGGGAAG ACCTACACAT GCCGATTACA ATCATTTGGG GGGTTACACC GATTGACAGC 
GGAGATCCGC TAAACCCTAA AAACAAGGGA AAGCTAACTC TTGACACAAC CTTCAACATT 
GCGAGTCCAG CGAGTCAAGT ATGGATTCTC AATTTCTGCC AGAAGCTCAG AAACCAGAGC 
TTCGTTTACC AATCCGAGGA GCAAGACTTC ACAAGCTGCT TCATGGAGAC TTTCAAGCAG 
TGGATGGAAA ACCAAGACTG CGAAGAAGCC TCTGTTTATC CCTGTTGTAG CCAATCCACG 
TTCCCCTACA GACAAGACGT CTTTGAGTTG TGCATCAAAC GGGCGATTAT GGAGCTCGAC 
CGAAGCACCA TCTACCACCT TGACAGCAAG ACTCCAGGAC CAAGGTTTGA CATAAATGAC 
ACCATTCGAG CTATCGTACT CGAGTTTCAA AGTACCTACC ATTTTACTCT GGCCTACGAG 
AAGATGCACC AGTTTTACAG GGAAGTTGAT ACTTGGATTC AGGAGGAGCT CAGAGATGCT 
CCTGAAGGAC TTAGCTATGG GTGGTTTGTA AGCAACCTGG AGTTCTACGA CCTACAGGAC 
AGCTTATCCG ACGGGACTTT AATTGCTATG GCACTGTCAG TGGTGGTGGC CTTCAGTGTT 
ATGCTCCTGA CCACCTGGAA CATCATCATC AGCCTCTACG CCATTATATC CATCGCCGGA 
ACTATATTTG TTACAGTCGG CTCACTGGTG CTTTTGGGCT GGGAGTTGAA TGTGCTGGAA 
TCAGTGACCA TATCAGTTGC TGTAGGGCTT TCTGTGGACT TTGCTGTACA TTATGGAGTG 
GCGTACCGCC TTGCTCCAGA ACCAGACAGG GAGGGGAAGG TGGTTTTCTC CTTGAGCAGG 
ATGGGCTCGG CTATAGCGAT GGCAGCATTG ACTACGTTTG TTGCTGGTGC AATGATGATG 
CCGTCCACTG TTCTGGCGTA TACCCAACTG GGCACATTCC TCATGCTAAT CATGTGCATT 
AGCTGGGCCT TTGCCACATT TTTCTTTCAA TGCATGTGCC GCCTGCTTGG ACCCCAAGGC 
ACTTGTGGGC AAATTCCATT GCCAAAGAAG CTGCAGTGTG CAGAGCGTAC ATCTGAAAAC 
CAGTCTACTG TGAATAACCA AGGTAAAGCT GGTGCTTGCT ACACAACCAA GCATGAACAC 
TATGAGCTTG AACCCTTAGC GGCGAATGTC AGAAATGAGG GAAAGGCCTC AGATGAGGAA 
CAAGAAGCCT GCACGCAGCT GCAGAATAAC ATCACTTCTC TTCATGATGA TGCAACCTTG 
TGTGCATCCC ATGAAGAACC CAAACATGAG TTTGAAAATG GCGTGCAAGC CACAGAACAT 
TCACATCTCC ATCAGTACGC TTTCAATACA AGGTGTACCT GTGAGAACTC AATGCATCAA 
TATATTCGGG AGCGACAAAG AACTGCTTGC CATCCTTGCG GCCAATCTCG AGAAACCCAA 
TGTGCTACTA AACAGTCCAA TCACTTACCT GCGTGTCTAA ACCGGGATAT GATCCATGCT 
CCTACCAACA GTGGCCACTT CCAGCATTGC ACACCAAGCC AAATATCAAG GCCAGCTGCT 
CATCGATGTT TCAGTGGGGG CTGTGGTATG CATGCAATGC ACATTTCACA ACAGCAAACA 
GAGACGGAGT CCTACATCAG TGCATGTGTC CCCAGTTCAT CTCCACCAGA GAGCCAGAAC 
CGTCCTGCTC ACATCCAGTT TCCAGATGTT CAACGCTGCT CTTCGCCTGA CACGACTGGA 
GTTTGTACTC TTAATCATGA TACACAGAAC GCTGGGGAAC TGGCGATAAA TTCCCAGAGT 
CCTCAGAAAA TACCATGGAA CCATTTAAAA ACAAAAGCAA ACGGCACTTG TGTGCTCCAG 
GATTGTCGTG AGGTGGTACC TGAAGACAGC ATCGGATGCA GAGTTGATAA AGAAAAGACT 
GTCTCTCCCA AAAAGTTTAA CTGTTTTAAG AAAAACGTTA AAGCAAAGTG CAATTCTGTA 
GAGTTTCACA AGACGAGAAC CAGCGTGCCT ACTATCGCCA TCAACTCGAA AGCCTCCTCT 
GAGAGTTTAT GCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017208012.1
CDS	164..4558
Translation	MALSEDFTELQTLSNGRSSSIFPTTNTPVDPEEEEEQSVSACTQPNEHTQMMQTGQNGGLKSTRLSSSSSSSSQLTDAQRLAVLLPNALPPGFCVCCSLCCNGNQQDCALFQSAVPSAALHMGSCCVQGVPCFCMQHHWPEHLQNQPNMATLSPPRPFRFPKSYAELIADWPVVVLGICTVLIVVCALVGILVPDLPDFSNPLQGFEPRGTAIGQRLVTWNNMVKNTGYKATLANYPFKYADEQAKSHQDDRWSKDHYDRKKRQAEWDFSKDSFFCDVPGDSYSRIVFASAEGKNLWNIQAIKSMCNFDNTRVRSHPQYLDLCQRTTAASCCPSWTLGNYIAVLTNKSSCQKITERDVSHTLNILRSCAKFYHNGTLGPECWDMTTRKKDLKCGNLPRKCTKYNAVYQIFHFLVDKDFLNPKNTDYLPPNLKYSMLFAPTEKGETMMNIYLDNFENRNSSDGITTITGIEFGIKHSLFQDYLLTDTMYPAIAIVIVLVVMCVYTRSMFITLMTMFAIISSLIVSYFLYRVVFNFDFFPFMNLTALIILVGIGADDAFVLCDVWNYTKFDKPNSELSETVSITLQHAALSMFVTSFTTAAAFYANYVSNITAIRCFGVYAGTAILVNYILMVTWLPAVVVLHERYLVNLLTCSRTQNHQSQGAATFWTSLCQVVNKCLFSISEASRIFFEKVLPCVVIKLRYLWLLWFLALTVGGAYVVCVNPKMKLPSLELSEFQVFRSSHPFERYDAEYKKLFMFERVNHGEDLHMPITIIWGVTPIDSGDPLNPKNKGKLTLDTTFNIASPASQVWILNFCQKLRNQSFVYQSEEQDFTSCFMETFKQWMENQDCEEASVYPCCSQSTFPYRQDVFELCIKRAIMELDRSTIYHLDSKTPGPRFDINDTIRAIVLEFQSTYHFTLAYEKMHQFYREVDTWIQEELRDAPEGLSYGWFVSNLEFYDLQDSLSDGTLIAMALSVVVAFSVMLLTTWNIIISLYAIISIAGTIFVTVGSLVLLGWELNVLESVTISVAVGLSVDFAVHYGVAYRLAPEPDREGKVVFSLSRMGSAIAMAALTTFVAGAMMMPSTVLAYTQLGTFLMLIMCISWAFATFFFQCMCRLLGPQGTCGQIPLPKKLQCAERTSENQSTVNNQGKAGACYTTKHEHYELEPLAANVRNEGKASDEEQEACTQLQNNITSLHDDATLCASHEEPKHEFENGVQATEHSHLHQYAFNTRCTCENSMHQYIRERQRTACHPCGQSRETQCATKQSNHLPACLNRDMIHAPTNSGHFQHCTPSQISRPAAHRCFSGGCGMHAMHISQQQTETESYISACVPSSSPPESQNRPAHIQFPDVQRCSSPDTTGVCTLNHDTQNAGELAINSQSPQKIPWNHLKTKANGTCVLQDCREVVPEDSIGCRVDKEKTVSPKKFNCFKKNVKAKCNSVEFHKTRTSVPTIAINSKASSESLC

Target ORF information:

RefSeq Version	XM_017352523.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio dispatched homolog 1 (Drosophila) (disp1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017352523.1

ORF Insert Sequence:

ATGGCTCTGA GCGAGGACTT CACTGAGCTC CAGACCCTCA GCAATGGCAG ATCCTCCAGC 
ATTTTCCCCA CCACAAACAC ACCTGTAGAC CCGGAGGAGG AGGAGGAGCA GAGCGTTTCT 
GCATGCACAC AGCCCAATGA ACACACACAG ATGATGCAGA CAGGCCAGAA CGGCGGACTC 
AAAAGCACAC GTCTGTCTTC TTCCTCATCA TCATCATCAC AGCTCACAGA TGCTCAGCGA 
TTGGCTGTTC TGCTGCCCAA TGCTCTGCCT CCGGGTTTCT GTGTGTGCTG CTCGCTCTGT 
TGTAACGGGA ACCAGCAGGA TTGTGCTCTC TTCCAGAGCG CCGTGCCGTC CGCTGCCCTC 
CACATGGGCT CCTGCTGCGT CCAGGGCGTC CCCTGTTTCT GCATGCAGCA TCACTGGCCG 
GAGCATCTGC AGAACCAGCC CAACATGGCC ACGCTGAGTC CTCCGAGGCC TTTCCGGTTT 
CCTAAAAGTT ATGCAGAGCT GATTGCTGAT TGGCCGGTGG TGGTGTTGGG GATTTGCACT 
GTCCTCATTG TGGTTTGTGC TCTGGTCGGG ATCCTCGTTC CTGATCTGCC TGACTTCTCA 
AACCCACTGC AGGGGTTTGA GCCGAGAGGC ACTGCAATAG GACAGAGACT GGTTACGTGG 
AACAACATGG TGAAAAACAC CGGCTACAAG GCAACACTGG CAAACTATCC ATTCAAATAC 
GCAGACGAGC AGGCCAAAAG TCACCAAGAT GACCGGTGGT CCAAAGATCA TTACGATCGA 
AAGAAAAGAC AAGCGGAGTG GGACTTCAGC AAAGATAGTT TCTTCTGTGA TGTACCAGGT 
GACAGCTATT CCCGGATCGT ATTTGCTTCT GCTGAGGGCA AGAACTTGTG GAATATACAG 
GCCATTAAAT CCATGTGCAA TTTCGACAAT ACGCGGGTTC GGTCTCATCC CCAGTATCTG 
GATCTGTGTC AGCGCACCAC TGCTGCCTCC TGCTGTCCCA GCTGGACCCT CGGAAACTAC 
ATAGCCGTCC TTACAAACAA ATCTTCCTGC CAGAAGATCA CAGAGCGAGA CGTTTCTCAC 
ACCCTGAATA TCCTGCGCTC CTGTGCTAAA TTTTACCACA ACGGTACCCT CGGTCCTGAA 
TGCTGGGACA TGACCACCCG AAAAAAGGAC CTCAAGTGTG GCAACCTGCC GCGGAAGTGT 
ACAAAATACA ACGCTGTGTA TCAGATTTTC CACTTTTTGG TGGACAAAGA CTTTCTGAAT 
CCAAAGAACA CAGACTACCT CCCACCAAAC CTCAAATACA GCATGCTCTT TGCTCCTACA 
GAGAAAGGAG AGACCATGAT GAACATTTAC TTGGACAACT TTGAGAACCG TAACTCTTCG 
GATGGGATCA CGACTATAAC GGGAATAGAA TTTGGCATCA AACACAGTTT GTTTCAGGAC 
TACCTCCTGA CAGATACCAT GTATCCCGCA ATAGCTATTG TCATCGTCTT GGTGGTCATG 
TGCGTCTACA CGAGATCCAT GTTCATCACC CTTATGACGA TGTTTGCGAT CATCAGTTCG 
CTCATCGTTT CTTATTTCCT ATATCGAGTG GTCTTCAACT TTGATTTCTT CCCTTTCATG 
AACCTAACAG CGCTCATCAT CCTAGTAGGA ATTGGGGCTG ATGATGCATT TGTGCTCTGC 
GACGTCTGGA ATTACACAAA GTTTGACAAA CCTAATTCTG AGCTCTCTGA AACGGTGAGC 
ATCACACTTC AGCATGCAGC GCTTTCAATG TTTGTCACTA GCTTCACAAC GGCTGCTGCC 
TTCTATGCCA ACTATGTGAG CAACATCACT GCCATACGAT GTTTCGGTGT CTATGCTGGA 
ACCGCTATCT TGGTTAACTA CATACTGATG GTTACCTGGT TGCCAGCTGT GGTGGTCTTA 
CATGAGCGGT ACCTGGTTAA CCTTCTTACA TGTTCACGAA CACAAAACCA TCAATCGCAG 
GGCGCTGCAA CGTTTTGGAC AAGCCTGTGC CAGGTGGTCA ACAAGTGTCT TTTTAGCATC 
TCTGAAGCCT CGAGGATTTT CTTCGAGAAA GTTCTGCCTT GTGTCGTGAT CAAGTTGCGC 
TACTTGTGGC TCTTGTGGTT CCTGGCTTTG ACAGTAGGTG GCGCTTATGT GGTTTGCGTA 
AATCCCAAAA TGAAACTTCC CTCTTTGGAG CTCTCAGAAT TTCAAGTGTT TCGCTCCTCG 
CATCCCTTTG AACGCTATGA TGCGGAGTAC AAAAAACTCT TCATGTTTGA ACGGGTCAAC 
CATGGGGAAG ACCTACACAT GCCGATTACA ATCATTTGGG GGGTTACACC GATTGACAGC 
GGAGATCCGC TAAACCCTAA AAACAAGGGA AAGCTAACTC TTGACACAAC CTTCAACATT 
GCGAGTCCAG CGAGTCAAGT ATGGATTCTC AATTTCTGCC AGAAGCTCAG AAACCAGAGC 
TTCGTTTACC AATCCGAGGA GCAAGACTTC ACAAGCTGCT TCATGGAGAC TTTCAAGCAG 
TGGATGGAAA ACCAAGACTG CGAAGAAGCC TCTGTTTATC CCTGTTGTAG CCAATCCACG 
TTCCCCTACA GACAAGACGT CTTTGAGTTG TGCATCAAAC GGGCGATTAT GGAGCTCGAC 
CGAAGCACCA TCTACCACCT TGACAGCAAG ACTCCAGGAC CAAGGTTTGA CATAAATGAC 
ACCATTCGAG CTATCGTACT CGAGTTTCAA AGTACCTACC ATTTTACTCT GGCCTACGAG 
AAGATGCACC AGTTTTACAG GGAAGTTGAT ACTTGGATTC AGGAGGAGCT CAGAGATGCT 
CCTGAAGGAC TTAGCTATGG GTGGTTTGTA AGCAACCTGG AGTTCTACGA CCTACAGGAC 
AGCTTATCCG ACGGGACTTT AATTGCTATG GCACTGTCAG TGGTGGTGGC CTTCAGTGTT 
ATGCTCCTGA CCACCTGGAA CATCATCATC AGCCTCTACG CCATTATATC CATCGCCGGA 
ACTATATTTG TTACAGTCGG CTCACTGGTG CTTTTGGGCT GGGAGTTGAA TGTGCTGGAA 
TCAGTGACCA TATCAGTTGC TGTAGGGCTT TCTGTGGACT TTGCTGTACA TTATGGAGTG 
GCGTACCGCC TTGCTCCAGA ACCAGACAGG GAGGGGAAGG TGGTTTTCTC CTTGAGCAGG 
ATGGGCTCGG CTATAGCGAT GGCAGCATTG ACTACGTTTG TTGCTGGTGC AATGATGATG 
CCGTCCACTG TTCTGGCGTA TACCCAACTG GGCACATTCC TCATGCTAAT CATGTGCATT 
AGCTGGGCCT TTGCCACATT TTTCTTTCAA TGCATGTGCC GCCTGCTTGG ACCCCAAGGC 
ACTTGTGGGC AAATTCCATT GCCAAAGAAG CTGCAGTGTG CAGAGCGTAC ATCTGAAAAC 
CAGTCTACTG TGAATAACCA AGGTAAAGCT GGTGCTTGCT ACACAACCAA GCATGAACAC 
TATGAGCTTG AACCCTTAGC GGCGAATGTC AGAAATGAGG GAAAGGCCTC AGATGAGGAA 
CAAGAAGCCT GCACGCAGCT GCAGAATAAC ATCACTTCTC TTCATGATGA TGCAACCTTG 
TGTGCATCCC ATGAAGAACC CAAACATGAG TTTGAAAATG GCGTGCAAGC CACAGAACAT 
TCACATCTCC ATCAGTACGC TTTCAATACA AGGTGTACCT GTGAGAACTC AATGCATCAA 
TATATTCGGG AGCGACAAAG AACTGCTTGC CATCCTTGCG GCCAATCTCG AGAAACCCAA 
TGTGCTACTA AACAGTCCAA TCACTTACCT GCGTGTCTAA ACCGGGATAT GATCCATGCT 
CCTACCAACA GTGGCCACTT CCAGCATTGC ACACCAAGCC AAATATCAAG GCCAGCTGCT 
CATCGATGTT TCAGTGGGGG CTGTGGTATG CATGCAATGC ACATTTCACA ACAGCAAACA 
GAGACGGAGT CCTACATCAG TGCATGTGTC CCCAGTTCAT CTCCACCAGA GAGCCAGAAC 
CGTCCTGCTC ACATCCAGTT TCCAGATGTT CAACGCTGCT CTTCGCCTGA CACGACTGGA 
GTTTGTACTC TTAATCATGA TACACAGAAC GCTGGGGAAC TGGCGATAAA TTCCCAGAGT 
CCTCAGAAAA TACCATGGAA CCATTTAAAA ACAAAAGCAA ACGGCACTTG TGTGCTCCAG 
GATTGTCGTG AGGTGGTACC TGAAGACAGC ATCGGATGCA GAGTTGATAA AGAAAAGACT 
GTCTCTCCCA AAAAGTTTAA CTGTTTTAAG AAAAACGTTA AAGCAAAGTG CAATTCTGTA 
GAGTTTCACA AGACGAGAAC CAGCGTGCCT ACTATCGCCA TCAACTCGAA AGCCTCCTCT 
GAGAGTTTAT GCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

disp1 ( XM_017352524.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017208013.1 Danio rerio dispatched homolog 1 (Drosophila) (disp1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa56588
Clone ID Related Accession (Same CDS sequence)	XM_017352523.1 , XM_017352524.1 , XM_017352523.2
Accession Version	XM_017352524.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4395bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	protein dispatched homolog 1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCTGA GCGAGGACTT CACTGAGCTC CAGACCCTCA GCAATGGCAG ATCCTCCAGC 
ATTTTCCCCA CCACAAACAC ACCTGTAGAC CCGGAGGAGG AGGAGGAGCA GAGCGTTTCT 
GCATGCACAC AGCCCAATGA ACACACACAG ATGATGCAGA CAGGCCAGAA CGGCGGACTC 
AAAAGCACAC GTCTGTCTTC TTCCTCATCA TCATCATCAC AGCTCACAGA TGCTCAGCGA 
TTGGCTGTTC TGCTGCCCAA TGCTCTGCCT CCGGGTTTCT GTGTGTGCTG CTCGCTCTGT 
TGTAACGGGA ACCAGCAGGA TTGTGCTCTC TTCCAGAGCG CCGTGCCGTC CGCTGCCCTC 
CACATGGGCT CCTGCTGCGT CCAGGGCGTC CCCTGTTTCT GCATGCAGCA TCACTGGCCG 
GAGCATCTGC AGAACCAGCC CAACATGGCC ACGCTGAGTC CTCCGAGGCC TTTCCGGTTT 
CCTAAAAGTT ATGCAGAGCT GATTGCTGAT TGGCCGGTGG TGGTGTTGGG GATTTGCACT 
GTCCTCATTG TGGTTTGTGC TCTGGTCGGG ATCCTCGTTC CTGATCTGCC TGACTTCTCA 
AACCCACTGC AGGGGTTTGA GCCGAGAGGC ACTGCAATAG GACAGAGACT GGTTACGTGG 
AACAACATGG TGAAAAACAC CGGCTACAAG GCAACACTGG CAAACTATCC ATTCAAATAC 
GCAGACGAGC AGGCCAAAAG TCACCAAGAT GACCGGTGGT CCAAAGATCA TTACGATCGA 
AAGAAAAGAC AAGCGGAGTG GGACTTCAGC AAAGATAGTT TCTTCTGTGA TGTACCAGGT 
GACAGCTATT CCCGGATCGT ATTTGCTTCT GCTGAGGGCA AGAACTTGTG GAATATACAG 
GCCATTAAAT CCATGTGCAA TTTCGACAAT ACGCGGGTTC GGTCTCATCC CCAGTATCTG 
GATCTGTGTC AGCGCACCAC TGCTGCCTCC TGCTGTCCCA GCTGGACCCT CGGAAACTAC 
ATAGCCGTCC TTACAAACAA ATCTTCCTGC CAGAAGATCA CAGAGCGAGA CGTTTCTCAC 
ACCCTGAATA TCCTGCGCTC CTGTGCTAAA TTTTACCACA ACGGTACCCT CGGTCCTGAA 
TGCTGGGACA TGACCACCCG AAAAAAGGAC CTCAAGTGTG GCAACCTGCC GCGGAAGTGT 
ACAAAATACA ACGCTGTGTA TCAGATTTTC CACTTTTTGG TGGACAAAGA CTTTCTGAAT 
CCAAAGAACA CAGACTACCT CCCACCAAAC CTCAAATACA GCATGCTCTT TGCTCCTACA 
GAGAAAGGAG AGACCATGAT GAACATTTAC TTGGACAACT TTGAGAACCG TAACTCTTCG 
GATGGGATCA CGACTATAAC GGGAATAGAA TTTGGCATCA AACACAGTTT GTTTCAGGAC 
TACCTCCTGA CAGATACCAT GTATCCCGCA ATAGCTATTG TCATCGTCTT GGTGGTCATG 
TGCGTCTACA CGAGATCCAT GTTCATCACC CTTATGACGA TGTTTGCGAT CATCAGTTCG 
CTCATCGTTT CTTATTTCCT ATATCGAGTG GTCTTCAACT TTGATTTCTT CCCTTTCATG 
AACCTAACAG CGCTCATCAT CCTAGTAGGA ATTGGGGCTG ATGATGCATT TGTGCTCTGC 
GACGTCTGGA ATTACACAAA GTTTGACAAA CCTAATTCTG AGCTCTCTGA AACGGTGAGC 
ATCACACTTC AGCATGCAGC GCTTTCAATG TTTGTCACTA GCTTCACAAC GGCTGCTGCC 
TTCTATGCCA ACTATGTGAG CAACATCACT GCCATACGAT GTTTCGGTGT CTATGCTGGA 
ACCGCTATCT TGGTTAACTA CATACTGATG GTTACCTGGT TGCCAGCTGT GGTGGTCTTA 
CATGAGCGGT ACCTGGTTAA CCTTCTTACA TGTTCACGAA CACAAAACCA TCAATCGCAG 
GGCGCTGCAA CGTTTTGGAC AAGCCTGTGC CAGGTGGTCA ACAAGTGTCT TTTTAGCATC 
TCTGAAGCCT CGAGGATTTT CTTCGAGAAA GTTCTGCCTT GTGTCGTGAT CAAGTTGCGC 
TACTTGTGGC TCTTGTGGTT CCTGGCTTTG ACAGTAGGTG GCGCTTATGT GGTTTGCGTA 
AATCCCAAAA TGAAACTTCC CTCTTTGGAG CTCTCAGAAT TTCAAGTGTT TCGCTCCTCG 
CATCCCTTTG AACGCTATGA TGCGGAGTAC AAAAAACTCT TCATGTTTGA ACGGGTCAAC 
CATGGGGAAG ACCTACACAT GCCGATTACA ATCATTTGGG GGGTTACACC GATTGACAGC 
GGAGATCCGC TAAACCCTAA AAACAAGGGA AAGCTAACTC TTGACACAAC CTTCAACATT 
GCGAGTCCAG CGAGTCAAGT ATGGATTCTC AATTTCTGCC AGAAGCTCAG AAACCAGAGC 
TTCGTTTACC AATCCGAGGA GCAAGACTTC ACAAGCTGCT TCATGGAGAC TTTCAAGCAG 
TGGATGGAAA ACCAAGACTG CGAAGAAGCC TCTGTTTATC CCTGTTGTAG CCAATCCACG 
TTCCCCTACA GACAAGACGT CTTTGAGTTG TGCATCAAAC GGGCGATTAT GGAGCTCGAC 
CGAAGCACCA TCTACCACCT TGACAGCAAG ACTCCAGGAC CAAGGTTTGA CATAAATGAC 
ACCATTCGAG CTATCGTACT CGAGTTTCAA AGTACCTACC ATTTTACTCT GGCCTACGAG 
AAGATGCACC AGTTTTACAG GGAAGTTGAT ACTTGGATTC AGGAGGAGCT CAGAGATGCT 
CCTGAAGGAC TTAGCTATGG GTGGTTTGTA AGCAACCTGG AGTTCTACGA CCTACAGGAC 
AGCTTATCCG ACGGGACTTT AATTGCTATG GCACTGTCAG TGGTGGTGGC CTTCAGTGTT 
ATGCTCCTGA CCACCTGGAA CATCATCATC AGCCTCTACG CCATTATATC CATCGCCGGA 
ACTATATTTG TTACAGTCGG CTCACTGGTG CTTTTGGGCT GGGAGTTGAA TGTGCTGGAA 
TCAGTGACCA TATCAGTTGC TGTAGGGCTT TCTGTGGACT TTGCTGTACA TTATGGAGTG 
GCGTACCGCC TTGCTCCAGA ACCAGACAGG GAGGGGAAGG TGGTTTTCTC CTTGAGCAGG 
ATGGGCTCGG CTATAGCGAT GGCAGCATTG ACTACGTTTG TTGCTGGTGC AATGATGATG 
CCGTCCACTG TTCTGGCGTA TACCCAACTG GGCACATTCC TCATGCTAAT CATGTGCATT 
AGCTGGGCCT TTGCCACATT TTTCTTTCAA TGCATGTGCC GCCTGCTTGG ACCCCAAGGC 
ACTTGTGGGC AAATTCCATT GCCAAAGAAG CTGCAGTGTG CAGAGCGTAC ATCTGAAAAC 
CAGTCTACTG TGAATAACCA AGGTAAAGCT GGTGCTTGCT ACACAACCAA GCATGAACAC 
TATGAGCTTG AACCCTTAGC GGCGAATGTC AGAAATGAGG GAAAGGCCTC AGATGAGGAA 
CAAGAAGCCT GCACGCAGCT GCAGAATAAC ATCACTTCTC TTCATGATGA TGCAACCTTG 
TGTGCATCCC ATGAAGAACC CAAACATGAG TTTGAAAATG GCGTGCAAGC CACAGAACAT 
TCACATCTCC ATCAGTACGC TTTCAATACA AGGTGTACCT GTGAGAACTC AATGCATCAA 
TATATTCGGG AGCGACAAAG AACTGCTTGC CATCCTTGCG GCCAATCTCG AGAAACCCAA 
TGTGCTACTA AACAGTCCAA TCACTTACCT GCGTGTCTAA ACCGGGATAT GATCCATGCT 
CCTACCAACA GTGGCCACTT CCAGCATTGC ACACCAAGCC AAATATCAAG GCCAGCTGCT 
CATCGATGTT TCAGTGGGGG CTGTGGTATG CATGCAATGC ACATTTCACA ACAGCAAACA 
GAGACGGAGT CCTACATCAG TGCATGTGTC CCCAGTTCAT CTCCACCAGA GAGCCAGAAC 
CGTCCTGCTC ACATCCAGTT TCCAGATGTT CAACGCTGCT CTTCGCCTGA CACGACTGGA 
GTTTGTACTC TTAATCATGA TACACAGAAC GCTGGGGAAC TGGCGATAAA TTCCCAGAGT 
CCTCAGAAAA TACCATGGAA CCATTTAAAA ACAAAAGCAA ACGGCACTTG TGTGCTCCAG 
GATTGTCGTG AGGTGGTACC TGAAGACAGC ATCGGATGCA GAGTTGATAA AGAAAAGACT 
GTCTCTCCCA AAAAGTTTAA CTGTTTTAAG AAAAACGTTA AAGCAAAGTG CAATTCTGTA 
GAGTTTCACA AGACGAGAAC CAGCGTGCCT ACTATCGCCA TCAACTCGAA AGCCTCCTCT 
GAGAGTTTAT GCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017208013.1
CDS	160..4554
Translation	MALSEDFTELQTLSNGRSSSIFPTTNTPVDPEEEEEQSVSACTQPNEHTQMMQTGQNGGLKSTRLSSSSSSSSQLTDAQRLAVLLPNALPPGFCVCCSLCCNGNQQDCALFQSAVPSAALHMGSCCVQGVPCFCMQHHWPEHLQNQPNMATLSPPRPFRFPKSYAELIADWPVVVLGICTVLIVVCALVGILVPDLPDFSNPLQGFEPRGTAIGQRLVTWNNMVKNTGYKATLANYPFKYADEQAKSHQDDRWSKDHYDRKKRQAEWDFSKDSFFCDVPGDSYSRIVFASAEGKNLWNIQAIKSMCNFDNTRVRSHPQYLDLCQRTTAASCCPSWTLGNYIAVLTNKSSCQKITERDVSHTLNILRSCAKFYHNGTLGPECWDMTTRKKDLKCGNLPRKCTKYNAVYQIFHFLVDKDFLNPKNTDYLPPNLKYSMLFAPTEKGETMMNIYLDNFENRNSSDGITTITGIEFGIKHSLFQDYLLTDTMYPAIAIVIVLVVMCVYTRSMFITLMTMFAIISSLIVSYFLYRVVFNFDFFPFMNLTALIILVGIGADDAFVLCDVWNYTKFDKPNSELSETVSITLQHAALSMFVTSFTTAAAFYANYVSNITAIRCFGVYAGTAILVNYILMVTWLPAVVVLHERYLVNLLTCSRTQNHQSQGAATFWTSLCQVVNKCLFSISEASRIFFEKVLPCVVIKLRYLWLLWFLALTVGGAYVVCVNPKMKLPSLELSEFQVFRSSHPFERYDAEYKKLFMFERVNHGEDLHMPITIIWGVTPIDSGDPLNPKNKGKLTLDTTFNIASPASQVWILNFCQKLRNQSFVYQSEEQDFTSCFMETFKQWMENQDCEEASVYPCCSQSTFPYRQDVFELCIKRAIMELDRSTIYHLDSKTPGPRFDINDTIRAIVLEFQSTYHFTLAYEKMHQFYREVDTWIQEELRDAPEGLSYGWFVSNLEFYDLQDSLSDGTLIAMALSVVVAFSVMLLTTWNIIISLYAIISIAGTIFVTVGSLVLLGWELNVLESVTISVAVGLSVDFAVHYGVAYRLAPEPDREGKVVFSLSRMGSAIAMAALTTFVAGAMMMPSTVLAYTQLGTFLMLIMCISWAFATFFFQCMCRLLGPQGTCGQIPLPKKLQCAERTSENQSTVNNQGKAGACYTTKHEHYELEPLAANVRNEGKASDEEQEACTQLQNNITSLHDDATLCASHEEPKHEFENGVQATEHSHLHQYAFNTRCTCENSMHQYIRERQRTACHPCGQSRETQCATKQSNHLPACLNRDMIHAPTNSGHFQHCTPSQISRPAAHRCFSGGCGMHAMHISQQQTETESYISACVPSSSPPESQNRPAHIQFPDVQRCSSPDTTGVCTLNHDTQNAGELAINSQSPQKIPWNHLKTKANGTCVLQDCREVVPEDSIGCRVDKEKTVSPKKFNCFKKNVKAKCNSVEFHKTRTSVPTIAINSKASSESLC

Target ORF information:

RefSeq Version	XM_017352524.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio dispatched homolog 1 (Drosophila) (disp1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017352524.1

ORF Insert Sequence:

ATGGCTCTGA GCGAGGACTT CACTGAGCTC CAGACCCTCA GCAATGGCAG ATCCTCCAGC 
ATTTTCCCCA CCACAAACAC ACCTGTAGAC CCGGAGGAGG AGGAGGAGCA GAGCGTTTCT 
GCATGCACAC AGCCCAATGA ACACACACAG ATGATGCAGA CAGGCCAGAA CGGCGGACTC 
AAAAGCACAC GTCTGTCTTC TTCCTCATCA TCATCATCAC AGCTCACAGA TGCTCAGCGA 
TTGGCTGTTC TGCTGCCCAA TGCTCTGCCT CCGGGTTTCT GTGTGTGCTG CTCGCTCTGT 
TGTAACGGGA ACCAGCAGGA TTGTGCTCTC TTCCAGAGCG CCGTGCCGTC CGCTGCCCTC 
CACATGGGCT CCTGCTGCGT CCAGGGCGTC CCCTGTTTCT GCATGCAGCA TCACTGGCCG 
GAGCATCTGC AGAACCAGCC CAACATGGCC ACGCTGAGTC CTCCGAGGCC TTTCCGGTTT 
CCTAAAAGTT ATGCAGAGCT GATTGCTGAT TGGCCGGTGG TGGTGTTGGG GATTTGCACT 
GTCCTCATTG TGGTTTGTGC TCTGGTCGGG ATCCTCGTTC CTGATCTGCC TGACTTCTCA 
AACCCACTGC AGGGGTTTGA GCCGAGAGGC ACTGCAATAG GACAGAGACT GGTTACGTGG 
AACAACATGG TGAAAAACAC CGGCTACAAG GCAACACTGG CAAACTATCC ATTCAAATAC 
GCAGACGAGC AGGCCAAAAG TCACCAAGAT GACCGGTGGT CCAAAGATCA TTACGATCGA 
AAGAAAAGAC AAGCGGAGTG GGACTTCAGC AAAGATAGTT TCTTCTGTGA TGTACCAGGT 
GACAGCTATT CCCGGATCGT ATTTGCTTCT GCTGAGGGCA AGAACTTGTG GAATATACAG 
GCCATTAAAT CCATGTGCAA TTTCGACAAT ACGCGGGTTC GGTCTCATCC CCAGTATCTG 
GATCTGTGTC AGCGCACCAC TGCTGCCTCC TGCTGTCCCA GCTGGACCCT CGGAAACTAC 
ATAGCCGTCC TTACAAACAA ATCTTCCTGC CAGAAGATCA CAGAGCGAGA CGTTTCTCAC 
ACCCTGAATA TCCTGCGCTC CTGTGCTAAA TTTTACCACA ACGGTACCCT CGGTCCTGAA 
TGCTGGGACA TGACCACCCG AAAAAAGGAC CTCAAGTGTG GCAACCTGCC GCGGAAGTGT 
ACAAAATACA ACGCTGTGTA TCAGATTTTC CACTTTTTGG TGGACAAAGA CTTTCTGAAT 
CCAAAGAACA CAGACTACCT CCCACCAAAC CTCAAATACA GCATGCTCTT TGCTCCTACA 
GAGAAAGGAG AGACCATGAT GAACATTTAC TTGGACAACT TTGAGAACCG TAACTCTTCG 
GATGGGATCA CGACTATAAC GGGAATAGAA TTTGGCATCA AACACAGTTT GTTTCAGGAC 
TACCTCCTGA CAGATACCAT GTATCCCGCA ATAGCTATTG TCATCGTCTT GGTGGTCATG 
TGCGTCTACA CGAGATCCAT GTTCATCACC CTTATGACGA TGTTTGCGAT CATCAGTTCG 
CTCATCGTTT CTTATTTCCT ATATCGAGTG GTCTTCAACT TTGATTTCTT CCCTTTCATG 
AACCTAACAG CGCTCATCAT CCTAGTAGGA ATTGGGGCTG ATGATGCATT TGTGCTCTGC 
GACGTCTGGA ATTACACAAA GTTTGACAAA CCTAATTCTG AGCTCTCTGA AACGGTGAGC 
ATCACACTTC AGCATGCAGC GCTTTCAATG TTTGTCACTA GCTTCACAAC GGCTGCTGCC 
TTCTATGCCA ACTATGTGAG CAACATCACT GCCATACGAT GTTTCGGTGT CTATGCTGGA 
ACCGCTATCT TGGTTAACTA CATACTGATG GTTACCTGGT TGCCAGCTGT GGTGGTCTTA 
CATGAGCGGT ACCTGGTTAA CCTTCTTACA TGTTCACGAA CACAAAACCA TCAATCGCAG 
GGCGCTGCAA CGTTTTGGAC AAGCCTGTGC CAGGTGGTCA ACAAGTGTCT TTTTAGCATC 
TCTGAAGCCT CGAGGATTTT CTTCGAGAAA GTTCTGCCTT GTGTCGTGAT CAAGTTGCGC 
TACTTGTGGC TCTTGTGGTT CCTGGCTTTG ACAGTAGGTG GCGCTTATGT GGTTTGCGTA 
AATCCCAAAA TGAAACTTCC CTCTTTGGAG CTCTCAGAAT TTCAAGTGTT TCGCTCCTCG 
CATCCCTTTG AACGCTATGA TGCGGAGTAC AAAAAACTCT TCATGTTTGA ACGGGTCAAC 
CATGGGGAAG ACCTACACAT GCCGATTACA ATCATTTGGG GGGTTACACC GATTGACAGC 
GGAGATCCGC TAAACCCTAA AAACAAGGGA AAGCTAACTC TTGACACAAC CTTCAACATT 
GCGAGTCCAG CGAGTCAAGT ATGGATTCTC AATTTCTGCC AGAAGCTCAG AAACCAGAGC 
TTCGTTTACC AATCCGAGGA GCAAGACTTC ACAAGCTGCT TCATGGAGAC TTTCAAGCAG 
TGGATGGAAA ACCAAGACTG CGAAGAAGCC TCTGTTTATC CCTGTTGTAG CCAATCCACG 
TTCCCCTACA GACAAGACGT CTTTGAGTTG TGCATCAAAC GGGCGATTAT GGAGCTCGAC 
CGAAGCACCA TCTACCACCT TGACAGCAAG ACTCCAGGAC CAAGGTTTGA CATAAATGAC 
ACCATTCGAG CTATCGTACT CGAGTTTCAA AGTACCTACC ATTTTACTCT GGCCTACGAG 
AAGATGCACC AGTTTTACAG GGAAGTTGAT ACTTGGATTC AGGAGGAGCT CAGAGATGCT 
CCTGAAGGAC TTAGCTATGG GTGGTTTGTA AGCAACCTGG AGTTCTACGA CCTACAGGAC 
AGCTTATCCG ACGGGACTTT AATTGCTATG GCACTGTCAG TGGTGGTGGC CTTCAGTGTT 
ATGCTCCTGA CCACCTGGAA CATCATCATC AGCCTCTACG CCATTATATC CATCGCCGGA 
ACTATATTTG TTACAGTCGG CTCACTGGTG CTTTTGGGCT GGGAGTTGAA TGTGCTGGAA 
TCAGTGACCA TATCAGTTGC TGTAGGGCTT TCTGTGGACT TTGCTGTACA TTATGGAGTG 
GCGTACCGCC TTGCTCCAGA ACCAGACAGG GAGGGGAAGG TGGTTTTCTC CTTGAGCAGG 
ATGGGCTCGG CTATAGCGAT GGCAGCATTG ACTACGTTTG TTGCTGGTGC AATGATGATG 
CCGTCCACTG TTCTGGCGTA TACCCAACTG GGCACATTCC TCATGCTAAT CATGTGCATT 
AGCTGGGCCT TTGCCACATT TTTCTTTCAA TGCATGTGCC GCCTGCTTGG ACCCCAAGGC 
ACTTGTGGGC AAATTCCATT GCCAAAGAAG CTGCAGTGTG CAGAGCGTAC ATCTGAAAAC 
CAGTCTACTG TGAATAACCA AGGTAAAGCT GGTGCTTGCT ACACAACCAA GCATGAACAC 
TATGAGCTTG AACCCTTAGC GGCGAATGTC AGAAATGAGG GAAAGGCCTC AGATGAGGAA 
CAAGAAGCCT GCACGCAGCT GCAGAATAAC ATCACTTCTC TTCATGATGA TGCAACCTTG 
TGTGCATCCC ATGAAGAACC CAAACATGAG TTTGAAAATG GCGTGCAAGC CACAGAACAT 
TCACATCTCC ATCAGTACGC TTTCAATACA AGGTGTACCT GTGAGAACTC AATGCATCAA 
TATATTCGGG AGCGACAAAG AACTGCTTGC CATCCTTGCG GCCAATCTCG AGAAACCCAA 
TGTGCTACTA AACAGTCCAA TCACTTACCT GCGTGTCTAA ACCGGGATAT GATCCATGCT 
CCTACCAACA GTGGCCACTT CCAGCATTGC ACACCAAGCC AAATATCAAG GCCAGCTGCT 
CATCGATGTT TCAGTGGGGG CTGTGGTATG CATGCAATGC ACATTTCACA ACAGCAAACA 
GAGACGGAGT CCTACATCAG TGCATGTGTC CCCAGTTCAT CTCCACCAGA GAGCCAGAAC 
CGTCCTGCTC ACATCCAGTT TCCAGATGTT CAACGCTGCT CTTCGCCTGA CACGACTGGA 
GTTTGTACTC TTAATCATGA TACACAGAAC GCTGGGGAAC TGGCGATAAA TTCCCAGAGT 
CCTCAGAAAA TACCATGGAA CCATTTAAAA ACAAAAGCAA ACGGCACTTG TGTGCTCCAG 
GATTGTCGTG AGGTGGTACC TGAAGACAGC ATCGGATGCA GAGTTGATAA AGAAAAGACT 
GTCTCTCCCA AAAAGTTTAA CTGTTTTAAG AAAAACGTTA AAGCAAAGTG CAATTCTGTA 
GAGTTTCACA AGACGAGAAC CAGCGTGCCT ACTATCGCCA TCAACTCGAA AGCCTCCTCT 
GAGAGTTTAT GCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

disp1 ( XM_017352523.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017208012.1 Danio rerio dispatched homolog 1 (Drosophila) (disp1), transcript variant X1, mRNA.

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CloneID	ODa56588
Clone ID Related Accession (Same CDS sequence)	XM_017352523.1 , XM_017352524.1 , XM_017352523.2
Accession Version	XM_017352523.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4395bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	protein dispatched homolog 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017352523.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCTGA GCGAGGACTT CACTGAGCTC CAGACCCTCA GCAATGGCAG ATCCTCCAGC 
ATTTTCCCCA CCACAAACAC ACCTGTAGAC CCGGAGGAGG AGGAGGAGCA GAGCGTTTCT 
GCATGCACAC AGCCCAATGA ACACACACAG ATGATGCAGA CAGGCCAGAA CGGCGGACTC 
AAAAGCACAC GTCTGTCTTC TTCCTCATCA TCATCATCAC AGCTCACAGA TGCTCAGCGA 
TTGGCTGTTC TGCTGCCCAA TGCTCTGCCT CCGGGTTTCT GTGTGTGCTG CTCGCTCTGT 
TGTAACGGGA ACCAGCAGGA TTGTGCTCTC TTCCAGAGCG CCGTGCCGTC CGCTGCCCTC 
CACATGGGCT CCTGCTGCGT CCAGGGCGTC CCCTGTTTCT GCATGCAGCA TCACTGGCCG 
GAGCATCTGC AGAACCAGCC CAACATGGCC ACGCTGAGTC CTCCGAGGCC TTTCCGGTTT 
CCTAAAAGTT ATGCAGAGCT GATTGCTGAT TGGCCGGTGG TGGTGTTGGG GATTTGCACT 
GTCCTCATTG TGGTTTGTGC TCTGGTCGGG ATCCTCGTTC CTGATCTGCC TGACTTCTCA 
AACCCACTGC AGGGGTTTGA GCCGAGAGGC ACTGCAATAG GACAGAGACT GGTTACGTGG 
AACAACATGG TGAAAAACAC CGGCTACAAG GCAACACTGG CAAACTATCC ATTCAAATAC 
GCAGACGAGC AGGCCAAAAG TCACCAAGAT GACCGGTGGT CCAAAGATCA TTACGATCGA 
AAGAAAAGAC AAGCGGAGTG GGACTTCAGC AAAGATAGTT TCTTCTGTGA TGTACCAGGT 
GACAGCTATT CCCGGATCGT ATTTGCTTCT GCTGAGGGCA AGAACTTGTG GAATATACAG 
GCCATTAAAT CCATGTGCAA TTTCGACAAT ACGCGGGTTC GGTCTCATCC CCAGTATCTG 
GATCTGTGTC AGCGCACCAC TGCTGCCTCC TGCTGTCCCA GCTGGACCCT CGGAAACTAC 
ATAGCCGTCC TTACAAACAA ATCTTCCTGC CAGAAGATCA CAGAGCGAGA CGTTTCTCAC 
ACCCTGAATA TCCTGCGCTC CTGTGCTAAA TTTTACCACA ACGGTACCCT CGGTCCTGAA 
TGCTGGGACA TGACCACCCG AAAAAAGGAC CTCAAGTGTG GCAACCTGCC GCGGAAGTGT 
ACAAAATACA ACGCTGTGTA TCAGATTTTC CACTTTTTGG TGGACAAAGA CTTTCTGAAT 
CCAAAGAACA CAGACTACCT CCCACCAAAC CTCAAATACA GCATGCTCTT TGCTCCTACA 
GAGAAAGGAG AGACCATGAT GAACATTTAC TTGGACAACT TTGAGAACCG TAACTCTTCG 
GATGGGATCA CGACTATAAC GGGAATAGAA TTTGGCATCA AACACAGTTT GTTTCAGGAC 
TACCTCCTGA CAGATACCAT GTATCCCGCA ATAGCTATTG TCATCGTCTT GGTGGTCATG 
TGCGTCTACA CGAGATCCAT GTTCATCACC CTTATGACGA TGTTTGCGAT CATCAGTTCG 
CTCATCGTTT CTTATTTCCT ATATCGAGTG GTCTTCAACT TTGATTTCTT CCCTTTCATG 
AACCTAACAG CGCTCATCAT CCTAGTAGGA ATTGGGGCTG ATGATGCATT TGTGCTCTGC 
GACGTCTGGA ATTACACAAA GTTTGACAAA CCTAATTCTG AGCTCTCTGA AACGGTGAGC 
ATCACACTTC AGCATGCAGC GCTTTCAATG TTTGTCACTA GCTTCACAAC GGCTGCTGCC 
TTCTATGCCA ACTATGTGAG CAACATCACT GCCATACGAT GTTTCGGTGT CTATGCTGGA 
ACCGCTATCT TGGTTAACTA CATACTGATG GTTACCTGGT TGCCAGCTGT GGTGGTCTTA 
CATGAGCGGT ACCTGGTTAA CCTTCTTACA TGTTCACGAA CACAAAACCA TCAATCGCAG 
GGCGCTGCAA CGTTTTGGAC AAGCCTGTGC CAGGTGGTCA ACAAGTGTCT TTTTAGCATC 
TCTGAAGCCT CGAGGATTTT CTTCGAGAAA GTTCTGCCTT GTGTCGTGAT CAAGTTGCGC 
TACTTGTGGC TCTTGTGGTT CCTGGCTTTG ACAGTAGGTG GCGCTTATGT GGTTTGCGTA 
AATCCCAAAA TGAAACTTCC CTCTTTGGAG CTCTCAGAAT TTCAAGTGTT TCGCTCCTCG 
CATCCCTTTG AACGCTATGA TGCGGAGTAC AAAAAACTCT TCATGTTTGA ACGGGTCAAC 
CATGGGGAAG ACCTACACAT GCCGATTACA ATCATTTGGG GGGTTACACC GATTGACAGC 
GGAGATCCGC TAAACCCTAA AAACAAGGGA AAGCTAACTC TTGACACAAC CTTCAACATT 
GCGAGTCCAG CGAGTCAAGT ATGGATTCTC AATTTCTGCC AGAAGCTCAG AAACCAGAGC 
TTCGTTTACC AATCCGAGGA GCAAGACTTC ACAAGCTGCT TCATGGAGAC TTTCAAGCAG 
TGGATGGAAA ACCAAGACTG CGAAGAAGCC TCTGTTTATC CCTGTTGTAG CCAATCCACG 
TTCCCCTACA GACAAGACGT CTTTGAGTTG TGCATCAAAC GGGCGATTAT GGAGCTCGAC 
CGAAGCACCA TCTACCACCT TGACAGCAAG ACTCCAGGAC CAAGGTTTGA CATAAATGAC 
ACCATTCGAG CTATCGTACT CGAGTTTCAA AGTACCTACC ATTTTACTCT GGCCTACGAG 
AAGATGCACC AGTTTTACAG GGAAGTTGAT ACTTGGATTC AGGAGGAGCT CAGAGATGCT 
CCTGAAGGAC TTAGCTATGG GTGGTTTGTA AGCAACCTGG AGTTCTACGA CCTACAGGAC 
AGCTTATCCG ACGGGACTTT AATTGCTATG GCACTGTCAG TGGTGGTGGC CTTCAGTGTT 
ATGCTCCTGA CCACCTGGAA CATCATCATC AGCCTCTACG CCATTATATC CATCGCCGGA 
ACTATATTTG TTACAGTCGG CTCACTGGTG CTTTTGGGCT GGGAGTTGAA TGTGCTGGAA 
TCAGTGACCA TATCAGTTGC TGTAGGGCTT TCTGTGGACT TTGCTGTACA TTATGGAGTG 
GCGTACCGCC TTGCTCCAGA ACCAGACAGG GAGGGGAAGG TGGTTTTCTC CTTGAGCAGG 
ATGGGCTCGG CTATAGCGAT GGCAGCATTG ACTACGTTTG TTGCTGGTGC AATGATGATG 
CCGTCCACTG TTCTGGCGTA TACCCAACTG GGCACATTCC TCATGCTAAT CATGTGCATT 
AGCTGGGCCT TTGCCACATT TTTCTTTCAA TGCATGTGCC GCCTGCTTGG ACCCCAAGGC 
ACTTGTGGGC AAATTCCATT GCCAAAGAAG CTGCAGTGTG CAGAGCGTAC ATCTGAAAAC 
CAGTCTACTG TGAATAACCA AGGTAAAGCT GGTGCTTGCT ACACAACCAA GCATGAACAC 
TATGAGCTTG AACCCTTAGC GGCGAATGTC AGAAATGAGG GAAAGGCCTC AGATGAGGAA 
CAAGAAGCCT GCACGCAGCT GCAGAATAAC ATCACTTCTC TTCATGATGA TGCAACCTTG 
TGTGCATCCC ATGAAGAACC CAAACATGAG TTTGAAAATG GCGTGCAAGC CACAGAACAT 
TCACATCTCC ATCAGTACGC TTTCAATACA AGGTGTACCT GTGAGAACTC AATGCATCAA 
TATATTCGGG AGCGACAAAG AACTGCTTGC CATCCTTGCG GCCAATCTCG AGAAACCCAA 
TGTGCTACTA AACAGTCCAA TCACTTACCT GCGTGTCTAA ACCGGGATAT GATCCATGCT 
CCTACCAACA GTGGCCACTT CCAGCATTGC ACACCAAGCC AAATATCAAG GCCAGCTGCT 
CATCGATGTT TCAGTGGGGG CTGTGGTATG CATGCAATGC ACATTTCACA ACAGCAAACA 
GAGACGGAGT CCTACATCAG TGCATGTGTC CCCAGTTCAT CTCCACCAGA GAGCCAGAAC 
CGTCCTGCTC ACATCCAGTT TCCAGATGTT CAACGCTGCT CTTCGCCTGA CACGACTGGA 
GTTTGTACTC TTAATCATGA TACACAGAAC GCTGGGGAAC TGGCGATAAA TTCCCAGAGT 
CCTCAGAAAA TACCATGGAA CCATTTAAAA ACAAAAGCAA ACGGCACTTG TGTGCTCCAG 
GATTGTCGTG AGGTGGTACC TGAAGACAGC ATCGGATGCA GAGTTGATAA AGAAAAGACT 
GTCTCTCCCA AAAAGTTTAA CTGTTTTAAG AAAAACGTTA AAGCAAAGTG CAATTCTGTA 
GAGTTTCACA AGACGAGAAC CAGCGTGCCT ACTATCGCCA TCAACTCGAA AGCCTCCTCT 
GAGAGTTTAT GCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017208012.1
CDS	177..4571
Translation	MALSEDFTELQTLSNGRSSSIFPTTNTPVDPEEEEEQSVSACTQPNEHTQMMQTGQNGGLKSTRLSSSSSSSSQLTDAQRLAVLLPNALPPGFCVCCSLCCNGNQQDCALFQSAVPSAALHMGSCCVQGVPCFCMQHHWPEHLQNQPNMATLSPPRPFRFPKSYAELIADWPVVVLGICTVLIVVCALVGILVPDLPDFSNPLQGFEPRGTAIGQRLVTWNNMVKNTGYKATLANYPFKYADEQAKSHQDDRWSKDHYDRKKRQAEWDFSKDSFFCDVPGDSYSRIVFASAEGKNLWNIQAIKSMCNFDNTRVRSHPQYLDLCQRTTAASCCPSWTLGNYIAVLTNKSSCQKITERDVSHTLNILRSCAKFYHNGTLGPECWDMTTRKKDLKCGNLPRKCTKYNAVYQIFHFLVDKDFLNPKNTDYLPPNLKYSMLFAPTEKGETMMNIYLDNFENRNSSDGITTITGIEFGIKHSLFQDYLLTDTMYPAIAIVIVLVVMCVYTRSMFITLMTMFAIISSLIVSYFLYRVVFNFDFFPFMNLTALIILVGIGADDAFVLCDVWNYTKFDKPNSELSETVSITLQHAALSMFVTSFTTAAAFYANYVSNITAIRCFGVYAGTAILVNYILMVTWLPAVVVLHERYLVNLLTCSRTQNHQSQGAATFWTSLCQVVNKCLFSISEASRIFFEKVLPCVVIKLRYLWLLWFLALTVGGAYVVCVNPKMKLPSLELSEFQVFRSSHPFERYDAEYKKLFMFERVNHGEDLHMPITIIWGVTPIDSGDPLNPKNKGKLTLDTTFNIASPASQVWILNFCQKLRNQSFVYQSEEQDFTSCFMETFKQWMENQDCEEASVYPCCSQSTFPYRQDVFELCIKRAIMELDRSTIYHLDSKTPGPRFDINDTIRAIVLEFQSTYHFTLAYEKMHQFYREVDTWIQEELRDAPEGLSYGWFVSNLEFYDLQDSLSDGTLIAMALSVVVAFSVMLLTTWNIIISLYAIISIAGTIFVTVGSLVLLGWELNVLESVTISVAVGLSVDFAVHYGVAYRLAPEPDREGKVVFSLSRMGSAIAMAALTTFVAGAMMMPSTVLAYTQLGTFLMLIMCISWAFATFFFQCMCRLLGPQGTCGQIPLPKKLQCAERTSENQSTVNNQGKAGACYTTKHEHYELEPLAANVRNEGKASDEEQEACTQLQNNITSLHDDATLCASHEEPKHEFENGVQATEHSHLHQYAFNTRCTCENSMHQYIRERQRTACHPCGQSRETQCATKQSNHLPACLNRDMIHAPTNSGHFQHCTPSQISRPAAHRCFSGGCGMHAMHISQQQTETESYISACVPSSSPPESQNRPAHIQFPDVQRCSSPDTTGVCTLNHDTQNAGELAINSQSPQKIPWNHLKTKANGTCVLQDCREVVPEDSIGCRVDKEKTVSPKKFNCFKKNVKAKCNSVEFHKTRTSVPTIAINSKASSESLC

Target ORF information:

RefSeq Version	XM_017352523.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio dispatched homolog 1 (Drosophila) (disp1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017352523.2

ORF Insert Sequence:

ATGGCTCTGA GCGAGGACTT CACTGAGCTC CAGACCCTCA GCAATGGCAG ATCCTCCAGC 
ATTTTCCCCA CCACAAACAC ACCTGTAGAC CCGGAGGAGG AGGAGGAGCA GAGCGTTTCT 
GCATGCACAC AGCCCAATGA ACACACACAG ATGATGCAGA CAGGCCAGAA CGGCGGACTC 
AAAAGCACAC GTCTGTCTTC TTCCTCATCA TCATCATCAC AGCTCACAGA TGCTCAGCGA 
TTGGCTGTTC TGCTGCCCAA TGCTCTGCCT CCGGGTTTCT GTGTGTGCTG CTCGCTCTGT 
TGTAACGGGA ACCAGCAGGA TTGTGCTCTC TTCCAGAGCG CCGTGCCGTC CGCTGCCCTC 
CACATGGGCT CCTGCTGCGT CCAGGGCGTC CCCTGTTTCT GCATGCAGCA TCACTGGCCG 
GAGCATCTGC AGAACCAGCC CAACATGGCC ACGCTGAGTC CTCCGAGGCC TTTCCGGTTT 
CCTAAAAGTT ATGCAGAGCT GATTGCTGAT TGGCCGGTGG TGGTGTTGGG GATTTGCACT 
GTCCTCATTG TGGTTTGTGC TCTGGTCGGG ATCCTCGTTC CTGATCTGCC TGACTTCTCA 
AACCCACTGC AGGGGTTTGA GCCGAGAGGC ACTGCAATAG GACAGAGACT GGTTACGTGG 
AACAACATGG TGAAAAACAC CGGCTACAAG GCAACACTGG CAAACTATCC ATTCAAATAC 
GCAGACGAGC AGGCCAAAAG TCACCAAGAT GACCGGTGGT CCAAAGATCA TTACGATCGA 
AAGAAAAGAC AAGCGGAGTG GGACTTCAGC AAAGATAGTT TCTTCTGTGA TGTACCAGGT 
GACAGCTATT CCCGGATCGT ATTTGCTTCT GCTGAGGGCA AGAACTTGTG GAATATACAG 
GCCATTAAAT CCATGTGCAA TTTCGACAAT ACGCGGGTTC GGTCTCATCC CCAGTATCTG 
GATCTGTGTC AGCGCACCAC TGCTGCCTCC TGCTGTCCCA GCTGGACCCT CGGAAACTAC 
ATAGCCGTCC TTACAAACAA ATCTTCCTGC CAGAAGATCA CAGAGCGAGA CGTTTCTCAC 
ACCCTGAATA TCCTGCGCTC CTGTGCTAAA TTTTACCACA ACGGTACCCT CGGTCCTGAA 
TGCTGGGACA TGACCACCCG AAAAAAGGAC CTCAAGTGTG GCAACCTGCC GCGGAAGTGT 
ACAAAATACA ACGCTGTGTA TCAGATTTTC CACTTTTTGG TGGACAAAGA CTTTCTGAAT 
CCAAAGAACA CAGACTACCT CCCACCAAAC CTCAAATACA GCATGCTCTT TGCTCCTACA 
GAGAAAGGAG AGACCATGAT GAACATTTAC TTGGACAACT TTGAGAACCG TAACTCTTCG 
GATGGGATCA CGACTATAAC GGGAATAGAA TTTGGCATCA AACACAGTTT GTTTCAGGAC 
TACCTCCTGA CAGATACCAT GTATCCCGCA ATAGCTATTG TCATCGTCTT GGTGGTCATG 
TGCGTCTACA CGAGATCCAT GTTCATCACC CTTATGACGA TGTTTGCGAT CATCAGTTCG 
CTCATCGTTT CTTATTTCCT ATATCGAGTG GTCTTCAACT TTGATTTCTT CCCTTTCATG 
AACCTAACAG CGCTCATCAT CCTAGTAGGA ATTGGGGCTG ATGATGCATT TGTGCTCTGC 
GACGTCTGGA ATTACACAAA GTTTGACAAA CCTAATTCTG AGCTCTCTGA AACGGTGAGC 
ATCACACTTC AGCATGCAGC GCTTTCAATG TTTGTCACTA GCTTCACAAC GGCTGCTGCC 
TTCTATGCCA ACTATGTGAG CAACATCACT GCCATACGAT GTTTCGGTGT CTATGCTGGA 
ACCGCTATCT TGGTTAACTA CATACTGATG GTTACCTGGT TGCCAGCTGT GGTGGTCTTA 
CATGAGCGGT ACCTGGTTAA CCTTCTTACA TGTTCACGAA CACAAAACCA TCAATCGCAG 
GGCGCTGCAA CGTTTTGGAC AAGCCTGTGC CAGGTGGTCA ACAAGTGTCT TTTTAGCATC 
TCTGAAGCCT CGAGGATTTT CTTCGAGAAA GTTCTGCCTT GTGTCGTGAT CAAGTTGCGC 
TACTTGTGGC TCTTGTGGTT CCTGGCTTTG ACAGTAGGTG GCGCTTATGT GGTTTGCGTA 
AATCCCAAAA TGAAACTTCC CTCTTTGGAG CTCTCAGAAT TTCAAGTGTT TCGCTCCTCG 
CATCCCTTTG AACGCTATGA TGCGGAGTAC AAAAAACTCT TCATGTTTGA ACGGGTCAAC 
CATGGGGAAG ACCTACACAT GCCGATTACA ATCATTTGGG GGGTTACACC GATTGACAGC 
GGAGATCCGC TAAACCCTAA AAACAAGGGA AAGCTAACTC TTGACACAAC CTTCAACATT 
GCGAGTCCAG CGAGTCAAGT ATGGATTCTC AATTTCTGCC AGAAGCTCAG AAACCAGAGC 
TTCGTTTACC AATCCGAGGA GCAAGACTTC ACAAGCTGCT TCATGGAGAC TTTCAAGCAG 
TGGATGGAAA ACCAAGACTG CGAAGAAGCC TCTGTTTATC CCTGTTGTAG CCAATCCACG 
TTCCCCTACA GACAAGACGT CTTTGAGTTG TGCATCAAAC GGGCGATTAT GGAGCTCGAC 
CGAAGCACCA TCTACCACCT TGACAGCAAG ACTCCAGGAC CAAGGTTTGA CATAAATGAC 
ACCATTCGAG CTATCGTACT CGAGTTTCAA AGTACCTACC ATTTTACTCT GGCCTACGAG 
AAGATGCACC AGTTTTACAG GGAAGTTGAT ACTTGGATTC AGGAGGAGCT CAGAGATGCT 
CCTGAAGGAC TTAGCTATGG GTGGTTTGTA AGCAACCTGG AGTTCTACGA CCTACAGGAC 
AGCTTATCCG ACGGGACTTT AATTGCTATG GCACTGTCAG TGGTGGTGGC CTTCAGTGTT 
ATGCTCCTGA CCACCTGGAA CATCATCATC AGCCTCTACG CCATTATATC CATCGCCGGA 
ACTATATTTG TTACAGTCGG CTCACTGGTG CTTTTGGGCT GGGAGTTGAA TGTGCTGGAA 
TCAGTGACCA TATCAGTTGC TGTAGGGCTT TCTGTGGACT TTGCTGTACA TTATGGAGTG 
GCGTACCGCC TTGCTCCAGA ACCAGACAGG GAGGGGAAGG TGGTTTTCTC CTTGAGCAGG 
ATGGGCTCGG CTATAGCGAT GGCAGCATTG ACTACGTTTG TTGCTGGTGC AATGATGATG 
CCGTCCACTG TTCTGGCGTA TACCCAACTG GGCACATTCC TCATGCTAAT CATGTGCATT 
AGCTGGGCCT TTGCCACATT TTTCTTTCAA TGCATGTGCC GCCTGCTTGG ACCCCAAGGC 
ACTTGTGGGC AAATTCCATT GCCAAAGAAG CTGCAGTGTG CAGAGCGTAC ATCTGAAAAC 
CAGTCTACTG TGAATAACCA AGGTAAAGCT GGTGCTTGCT ACACAACCAA GCATGAACAC 
TATGAGCTTG AACCCTTAGC GGCGAATGTC AGAAATGAGG GAAAGGCCTC AGATGAGGAA 
CAAGAAGCCT GCACGCAGCT GCAGAATAAC ATCACTTCTC TTCATGATGA TGCAACCTTG 
TGTGCATCCC ATGAAGAACC CAAACATGAG TTTGAAAATG GCGTGCAAGC CACAGAACAT 
TCACATCTCC ATCAGTACGC TTTCAATACA AGGTGTACCT GTGAGAACTC AATGCATCAA 
TATATTCGGG AGCGACAAAG AACTGCTTGC CATCCTTGCG GCCAATCTCG AGAAACCCAA 
TGTGCTACTA AACAGTCCAA TCACTTACCT GCGTGTCTAA ACCGGGATAT GATCCATGCT 
CCTACCAACA GTGGCCACTT CCAGCATTGC ACACCAAGCC AAATATCAAG GCCAGCTGCT 
CATCGATGTT TCAGTGGGGG CTGTGGTATG CATGCAATGC ACATTTCACA ACAGCAAACA 
GAGACGGAGT CCTACATCAG TGCATGTGTC CCCAGTTCAT CTCCACCAGA GAGCCAGAAC 
CGTCCTGCTC ACATCCAGTT TCCAGATGTT CAACGCTGCT CTTCGCCTGA CACGACTGGA 
GTTTGTACTC TTAATCATGA TACACAGAAC GCTGGGGAAC TGGCGATAAA TTCCCAGAGT 
CCTCAGAAAA TACCATGGAA CCATTTAAAA ACAAAAGCAA ACGGCACTTG TGTGCTCCAG 
GATTGTCGTG AGGTGGTACC TGAAGACAGC ATCGGATGCA GAGTTGATAA AGAAAAGACT 
GTCTCTCCCA AAAAGTTTAA CTGTTTTAAG AAAAACGTTA AAGCAAAGTG CAATTCTGTA 
GAGTTTCACA AGACGAGAAC CAGCGTGCCT ACTATCGCCA TCAACTCGAA AGCCTCCTCT 
GAGAGTTTAT GCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

disp1 ( NM_212800.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_997965.1 Danio rerio dispatched homolog 1 (Drosophila) (disp1), mRNA.

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CloneID	ODa38265
Clone ID Related Accession (Same CDS sequence)	NM_212800.1
Accession Version	NM_212800.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4395bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Danio rerio(zebrafish)
Product	protein dispatched homolog 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY512779.1. On Dec 17, 2008 this sequence version replaced NM_001033593.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY512779.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGGCTCTGA GCGAGGACTT CACTGAGCTC CAGACCCTCA GCAATGGCAG ATCCTCCAGC 
ATTTTCCCCA CCACAAACAC ACCTGTAGAC CCGGAGGAGG AGGAGGAGCA GAGCGTTTCT 
GCATGCACAC AGCCCAATGA ACACACACAG ATGATGCAGA CAGGCCAGAA CGGCGGACTC 
AAAAGCACAC GTCTGTCTTC TTCCTCATCA TCATCATCAC AGCTCACAGA TGCTCAGCGA 
TTGGCTGTTC TGCTGCCCAA TGCTTTGCCT CCGGGTTTCT GTGTGTGCTG CTCGCTCTGT 
TGTAACGGGA ACCAGCAGGA TTGTGCTCTC TTCCAGAGCG CCGTGCCGTC CGCTGCCCTC 
CACATGGGCT CCTGCTGCGT CCAGGGCGTC CCCTGTTTCT GCATGCAGCA TCACTGGCCG 
GAGCATCTGC AGAACCAGCC CAACATGGCC ACGCTGAGTC CTCCGAGGCC TTTCCGGTTT 
CCTAAAAATT ATGCAGAGCT GATTGCTGAT TGGCCGGTGG TGGTGTTGGG GATTTGCACC 
GTCCTCATTG TGGTTTGTGC TCTGGTCGGG ATCCTCGTTC CTGATCTGCC TGACTTCTCA 
AACCCACTGC AGGGGTTTGA GCCGAGAGGC ACCGCAATAG GACAGAGACT GGTTACATGG 
AACAACATGG TGAAAAACAC CGGCTACAAG GCAACACTGG CAAACTATCC ATTCAAATAC 
GCGGACGAGC AGGCCAAAAG TCACCAAGAT GACCGGTGGT CCAAAGATCA TTACGATCGA 
AAGAAAAGAC AAGCGGAGTG GGATTTCAGC AAAGATAGTT TCTTCTGTGA TGTACCAGGT 
GACAGCTATT CCCGGATCGT ATTTGCTTCT GCTGAGGGCA AGAACTTGTG GAATATACAG 
GCCATTAAAT CCATGTGCAA TTTCGACAAT ACGCGGGTTC GCTCTCATCC CCAGTATCTG 
GATCTGTGCC AGCGCACCAC TGCTGCCTCC TGCTGTCCCA GCTGGACCCT CGGAAACTAC 
ATAGCCGTCC TTACAAACAA ATCTTCCTGC CAGAAGATCA CAGAGCGAGA CGTTTCTCAC 
ACCCTGAATA TCCTGCGCTC CTGTGCTAAA TTTTACCACA ACGGTACCCT CGGTCCTGAA 
TGCTGGGACA TGACCACCCG AAAAAAGGAC CTCAAGTGTG GCAACCTGCC GCGGAAGTGT 
ACAAAATACA ACGCCGTGTA TCAGATTTTC CACTTTTTGG TGGACAAAGA CTTTCTGAAT 
CCAAAGAACA CAGACTACCT CCCACCAAAC CTCAAATACA GCATGCTCTT TGCTCCTACA 
GAGAAAGGAG AGACCATGAT GAACATTTAC TTGGACAACT TTGAGAACCG TAACTCTTCG 
GATGGGATCA CGACTATAAC GGGAATAGAA TTTGGCATCA AACACAGTTT GTTTCAGGAC 
TACCTCCTGA CAGATACCAT GTATCCCGCA ATAGCTATTG TCATCGTCTT GGTGGTCATG 
TGCGTCTACA CGAGATCCAT GTTCATCACC CTTATGACGA TGTTTGCGAT CATCAGTTCG 
CTCATCGTTT CTTATTTCCT ATATCGAGTG GTCTTCAACT TTGATTTCTT CCCTTTCATG 
AACCTAACAG CGCTCATCAT CCTAGTAGGA ATTGGGGCTG ATGATGCATT TGTGCTCTGC 
GACGTCTGGA ATTACACAAA GTTTGACAAA CCTAATTCTG AGCTCTCTGA AACGGTGAGC 
ATCACACTTC AGCATGCAGC GCTTTCAATG TTTGTCACTA GCTTCACAAC GGCTGCTGCC 
TTCTATGCCA ACTATGTGAG CAACATCACT GCCATACGAT GTTTCGGTGT CTATGCTGGA 
ACCGCTATCT TGGTTAACTA CATACTGATG GTTACCTGGT TGCCAGCTGT GGTGGTCTTA 
CATGAGCGGT ACCTGGTTAA CCTTCTTACA TGTTCACGAA CACAAAACCA TCAATCGCAG 
GGCGCTGCAA CGTTTTGGAC AAGCCTGTGC CAGGTGGTCA ACAAGTGTCT TTTTAGCATC 
TCTGAAGCCT CGAGGATTTT CTTCGAGAAA GTTCTGCCTT GTGTCGTGAT CAAGTTGCGC 
TACTTGTGGC TCTTGTGGTT CCTGGCTTTG ACAGTAGGTG GCGCTTATGT GGTTTGCGTA 
AATCCCAAAA TGAAACTTCC CTCTTTGGAG CTCTCAGAAT TTCAAGTGTT TCGCTCCTCG 
CATCCCTTTG AACGCTATGA TGCGGAGTAC AAAAAACTCT TCATGTTTGA ACGGGTCAAC 
CATGGGGAAG ACCTACACAT GCCGATTACA ATCATTTGGG GGGTTACACC GATTGACAGC 
GGAGATCCGC TAAACCCTAA AAACAAGGGA AAGCTAACTC TTGACACAAC CTTCAACATT 
GCGAGTCCAG CGAGTCAAGT ATGGATTCTC AATTTCTGCC AGAAGCTCAG AAACCAGAGC 
TTCGTTTACC AATCCGAGGA GCAAGACTTC ACAAGCTGCT TCATGGAGAC TTTCAAGCAG 
TGGATGGAAA ACCAAGACTG CGAAGAAGCC TCTGTTTATC CCTGTTGTAG CCAATCCACG 
TTCCCCTACA GACAAGACGT CTTTGAGTTG TGCATCAAAC GGGCGATTAT GGAGCTCGAC 
CGAAGCACCA TCTACCACCT TGACAGCAAG ACTCCAGGAC CAAGGTTTGA CATAAATGAC 
ACCATTCGAG CTATCGTACT CGAGTTTCAA AGTACCTACC ATTTTACTCT GGCCTACGAG 
AAGATGCACC AGTTTTACAG GGAAGTTGAT ACTTGGATTC AGGAGGAGCT CAGAGATGCT 
CCTGAAGGAC TTAGCTATGG GTGGTTTGTA AGCAACCTGG AGTTCTACGA CCTACAGGAC 
AGCTTATCCG ACGGGACTTT AATTGCTATG GCACTGTCAG TGGTGGTGGC CTTCAGTGTT 
ATGCTCCTGA CCACCTGGAA CATCATCATC AGCCTCTACG CCATTATATC CATCGCCGGA 
ACTATATTTG TTACAGTCGG CTCACTGGTG CTTTTGGGCT GGGAGTTGAA TGTGCTGGAA 
TCAGTGACCA TATCAGTTGC TGTAGGGCTT TCTGTGGACT TTGCTGTACA TTATGGAGTG 
GCGTACCGCC TTGCTCCAGA ACCAGACAGG GAGGGGAAGG TGGTTTTCTC CTTGAGCAGG 
ATGGGCTCGG CTATAGCGAT GGCAGCATTG ACTACGTTTG TTGCTGGTGC AATGATGATG 
CCGTCCACTG TTCTGGCGTA TACCCAACTG GGCACATTCC TCATGCTAAT CATGTGCATT 
AGCTGGGCCT TTGCCACATT TTTCTTTCAA TGCATGTGCC GCCTGCTTGG ACCCCAAGGC 
ACTTGTGGGC AAATTCCATT GCCAAAGAAG CTGCAGTGTG CAGAGCGTAC ATCTGAAAAC 
CAGTCTACTG TGAATAACCA AGGTAAAGCT GGTGCTTGCT ACACAACCAA GCATGAACAC 
TATGAGCTTG AACCCTTAGC GGCGAATGTC AGAAATGAGG GAAAGGCCTC AGATGAGGAA 
CAAGAAGCCT GCACGCAGCT GCAGAATAAC ATCACTTCTC TTCATGATGA TGCAACCTTG 
TGTGCATCCC ATGAAGAACC CAAACATGAG TTTGAAAATG GCGTGCAAGC CACAGAACAT 
TCACATCTCC ATCAGTACGC TTTCAATACA AGGTGTACCT GCGAGAACTC AATGCATCAG 
TATATTCGGG AGCGACAAAG AACTGCTTGC CATCCTTGCG GCCAATCTCG AGAAACCCAA 
TGTGCTACTA AACAGTCCAA TCACTTACCT GTGTGTCTAA ACCGGGATAT GATCCATGCT 
CCTACCAACA GTGGCCACTT CCAGCATTGC ACACCAAGCC AAATATCAAG GCCAGCTGCT 
CATCGATGTT TCAGTGGGGG CTGTGGTATG CATGCGGTGC ACATTTCACA ACAGCAAACA 
GAGACGGAGT CCTACATCAG TGCATGTGTC CCCAGTTCAT CTCCACAAGA GAGCCAGAAC 
CGTCCTGCTC ACCTCCAGTT TCCAGATGTT CAACGCTGCT CTTCGCCTGA CACGACTGGA 
GTTTGTACTC TTAATCACGA TACACAGAAC GCTGGGGAAC TGGCGATAAA TTCCCAGAGT 
CCTCAGAAAA TACCATGGAA CCATTTAAAA ACAAAAGCAA ACGGCACTTG TGTGCTCCAG 
GATTGTCGTG AGGTGGTACC TGAAGACAGC ATCGGATGCA GAGTTGATAA AGAAAAGACT 
GTCTCTCCCA AAAAGTTTAA CTGTTTTAAG AAAAACGTTA ACGCAAAGTG CAATTCTGTA 
GAGTTTCACA AGACGAGAAC CAGCGTGCCT ACTATCGCCA TCAACTCGAA AGCCTCCTCT 
GAGAGTTTAT GCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_997965.1
CDS	139..4533
Translation	MALSEDFTELQTLSNGRSSSIFPTTNTPVDPEEEEEQSVSACTQPNEHTQMMQTGQNGGLKSTRLSSSSSSSSQLTDAQRLAVLLPNALPPGFCVCCSLCCNGNQQDCALFQSAVPSAALHMGSCCVQGVPCFCMQHHWPEHLQNQPNMATLSPPRPFRFPKNYAELIADWPVVVLGICTVLIVVCALVGILVPDLPDFSNPLQGFEPRGTAIGQRLVTWNNMVKNTGYKATLANYPFKYADEQAKSHQDDRWSKDHYDRKKRQAEWDFSKDSFFCDVPGDSYSRIVFASAEGKNLWNIQAIKSMCNFDNTRVRSHPQYLDLCQRTTAASCCPSWTLGNYIAVLTNKSSCQKITERDVSHTLNILRSCAKFYHNGTLGPECWDMTTRKKDLKCGNLPRKCTKYNAVYQIFHFLVDKDFLNPKNTDYLPPNLKYSMLFAPTEKGETMMNIYLDNFENRNSSDGITTITGIEFGIKHSLFQDYLLTDTMYPAIAIVIVLVVMCVYTRSMFITLMTMFAIISSLIVSYFLYRVVFNFDFFPFMNLTALIILVGIGADDAFVLCDVWNYTKFDKPNSELSETVSITLQHAALSMFVTSFTTAAAFYANYVSNITAIRCFGVYAGTAILVNYILMVTWLPAVVVLHERYLVNLLTCSRTQNHQSQGAATFWTSLCQVVNKCLFSISEASRIFFEKVLPCVVIKLRYLWLLWFLALTVGGAYVVCVNPKMKLPSLELSEFQVFRSSHPFERYDAEYKKLFMFERVNHGEDLHMPITIIWGVTPIDSGDPLNPKNKGKLTLDTTFNIASPASQVWILNFCQKLRNQSFVYQSEEQDFTSCFMETFKQWMENQDCEEASVYPCCSQSTFPYRQDVFELCIKRAIMELDRSTIYHLDSKTPGPRFDINDTIRAIVLEFQSTYHFTLAYEKMHQFYREVDTWIQEELRDAPEGLSYGWFVSNLEFYDLQDSLSDGTLIAMALSVVVAFSVMLLTTWNIIISLYAIISIAGTIFVTVGSLVLLGWELNVLESVTISVAVGLSVDFAVHYGVAYRLAPEPDREGKVVFSLSRMGSAIAMAALTTFVAGAMMMPSTVLAYTQLGTFLMLIMCISWAFATFFFQCMCRLLGPQGTCGQIPLPKKLQCAERTSENQSTVNNQGKAGACYTTKHEHYELEPLAANVRNEGKASDEEQEACTQLQNNITSLHDDATLCASHEEPKHEFENGVQATEHSHLHQYAFNTRCTCENSMHQYIRERQRTACHPCGQSRETQCATKQSNHLPVCLNRDMIHAPTNSGHFQHCTPSQISRPAAHRCFSGGCGMHAVHISQQQTETESYISACVPSSSPQESQNRPAHLQFPDVQRCSSPDTTGVCTLNHDTQNAGELAINSQSPQKIPWNHLKTKANGTCVLQDCREVVPEDSIGCRVDKEKTVSPKKFNCFKKNVNAKCNSVEFHKTRTSVPTIAINSKASSESLC

Target ORF information:

RefSeq Version	NM_212800.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio dispatched homolog 1 (Drosophila) (disp1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_212800.1

ORF Insert Sequence:

ATGGCTCTGA GCGAGGACTT CACTGAGCTC CAGACCCTCA GCAATGGCAG ATCCTCCAGC 
ATTTTCCCCA CCACAAACAC ACCTGTAGAC CCGGAGGAGG AGGAGGAGCA GAGCGTTTCT 
GCATGCACAC AGCCCAATGA ACACACACAG ATGATGCAGA CAGGCCAGAA CGGCGGACTC 
AAAAGCACAC GTCTGTCTTC TTCCTCATCA TCATCATCAC AGCTCACAGA TGCTCAGCGA 
TTGGCTGTTC TGCTGCCCAA TGCTTTGCCT CCGGGTTTCT GTGTGTGCTG CTCGCTCTGT 
TGTAACGGGA ACCAGCAGGA TTGTGCTCTC TTCCAGAGCG CCGTGCCGTC CGCTGCCCTC 
CACATGGGCT CCTGCTGCGT CCAGGGCGTC CCCTGTTTCT GCATGCAGCA TCACTGGCCG 
GAGCATCTGC AGAACCAGCC CAACATGGCC ACGCTGAGTC CTCCGAGGCC TTTCCGGTTT 
CCTAAAAATT ATGCAGAGCT GATTGCTGAT TGGCCGGTGG TGGTGTTGGG GATTTGCACC 
GTCCTCATTG TGGTTTGTGC TCTGGTCGGG ATCCTCGTTC CTGATCTGCC TGACTTCTCA 
AACCCACTGC AGGGGTTTGA GCCGAGAGGC ACCGCAATAG GACAGAGACT GGTTACATGG 
AACAACATGG TGAAAAACAC CGGCTACAAG GCAACACTGG CAAACTATCC ATTCAAATAC 
GCGGACGAGC AGGCCAAAAG TCACCAAGAT GACCGGTGGT CCAAAGATCA TTACGATCGA 
AAGAAAAGAC AAGCGGAGTG GGATTTCAGC AAAGATAGTT TCTTCTGTGA TGTACCAGGT 
GACAGCTATT CCCGGATCGT ATTTGCTTCT GCTGAGGGCA AGAACTTGTG GAATATACAG 
GCCATTAAAT CCATGTGCAA TTTCGACAAT ACGCGGGTTC GCTCTCATCC CCAGTATCTG 
GATCTGTGCC AGCGCACCAC TGCTGCCTCC TGCTGTCCCA GCTGGACCCT CGGAAACTAC 
ATAGCCGTCC TTACAAACAA ATCTTCCTGC CAGAAGATCA CAGAGCGAGA CGTTTCTCAC 
ACCCTGAATA TCCTGCGCTC CTGTGCTAAA TTTTACCACA ACGGTACCCT CGGTCCTGAA 
TGCTGGGACA TGACCACCCG AAAAAAGGAC CTCAAGTGTG GCAACCTGCC GCGGAAGTGT 
ACAAAATACA ACGCCGTGTA TCAGATTTTC CACTTTTTGG TGGACAAAGA CTTTCTGAAT 
CCAAAGAACA CAGACTACCT CCCACCAAAC CTCAAATACA GCATGCTCTT TGCTCCTACA 
GAGAAAGGAG AGACCATGAT GAACATTTAC TTGGACAACT TTGAGAACCG TAACTCTTCG 
GATGGGATCA CGACTATAAC GGGAATAGAA TTTGGCATCA AACACAGTTT GTTTCAGGAC 
TACCTCCTGA CAGATACCAT GTATCCCGCA ATAGCTATTG TCATCGTCTT GGTGGTCATG 
TGCGTCTACA CGAGATCCAT GTTCATCACC CTTATGACGA TGTTTGCGAT CATCAGTTCG 
CTCATCGTTT CTTATTTCCT ATATCGAGTG GTCTTCAACT TTGATTTCTT CCCTTTCATG 
AACCTAACAG CGCTCATCAT CCTAGTAGGA ATTGGGGCTG ATGATGCATT TGTGCTCTGC 
GACGTCTGGA ATTACACAAA GTTTGACAAA CCTAATTCTG AGCTCTCTGA AACGGTGAGC 
ATCACACTTC AGCATGCAGC GCTTTCAATG TTTGTCACTA GCTTCACAAC GGCTGCTGCC 
TTCTATGCCA ACTATGTGAG CAACATCACT GCCATACGAT GTTTCGGTGT CTATGCTGGA 
ACCGCTATCT TGGTTAACTA CATACTGATG GTTACCTGGT TGCCAGCTGT GGTGGTCTTA 
CATGAGCGGT ACCTGGTTAA CCTTCTTACA TGTTCACGAA CACAAAACCA TCAATCGCAG 
GGCGCTGCAA CGTTTTGGAC AAGCCTGTGC CAGGTGGTCA ACAAGTGTCT TTTTAGCATC 
TCTGAAGCCT CGAGGATTTT CTTCGAGAAA GTTCTGCCTT GTGTCGTGAT CAAGTTGCGC 
TACTTGTGGC TCTTGTGGTT CCTGGCTTTG ACAGTAGGTG GCGCTTATGT GGTTTGCGTA 
AATCCCAAAA TGAAACTTCC CTCTTTGGAG CTCTCAGAAT TTCAAGTGTT TCGCTCCTCG 
CATCCCTTTG AACGCTATGA TGCGGAGTAC AAAAAACTCT TCATGTTTGA ACGGGTCAAC 
CATGGGGAAG ACCTACACAT GCCGATTACA ATCATTTGGG GGGTTACACC GATTGACAGC 
GGAGATCCGC TAAACCCTAA AAACAAGGGA AAGCTAACTC TTGACACAAC CTTCAACATT 
GCGAGTCCAG CGAGTCAAGT ATGGATTCTC AATTTCTGCC AGAAGCTCAG AAACCAGAGC 
TTCGTTTACC AATCCGAGGA GCAAGACTTC ACAAGCTGCT TCATGGAGAC TTTCAAGCAG 
TGGATGGAAA ACCAAGACTG CGAAGAAGCC TCTGTTTATC CCTGTTGTAG CCAATCCACG 
TTCCCCTACA GACAAGACGT CTTTGAGTTG TGCATCAAAC GGGCGATTAT GGAGCTCGAC 
CGAAGCACCA TCTACCACCT TGACAGCAAG ACTCCAGGAC CAAGGTTTGA CATAAATGAC 
ACCATTCGAG CTATCGTACT CGAGTTTCAA AGTACCTACC ATTTTACTCT GGCCTACGAG 
AAGATGCACC AGTTTTACAG GGAAGTTGAT ACTTGGATTC AGGAGGAGCT CAGAGATGCT 
CCTGAAGGAC TTAGCTATGG GTGGTTTGTA AGCAACCTGG AGTTCTACGA CCTACAGGAC 
AGCTTATCCG ACGGGACTTT AATTGCTATG GCACTGTCAG TGGTGGTGGC CTTCAGTGTT 
ATGCTCCTGA CCACCTGGAA CATCATCATC AGCCTCTACG CCATTATATC CATCGCCGGA 
ACTATATTTG TTACAGTCGG CTCACTGGTG CTTTTGGGCT GGGAGTTGAA TGTGCTGGAA 
TCAGTGACCA TATCAGTTGC TGTAGGGCTT TCTGTGGACT TTGCTGTACA TTATGGAGTG 
GCGTACCGCC TTGCTCCAGA ACCAGACAGG GAGGGGAAGG TGGTTTTCTC CTTGAGCAGG 
ATGGGCTCGG CTATAGCGAT GGCAGCATTG ACTACGTTTG TTGCTGGTGC AATGATGATG 
CCGTCCACTG TTCTGGCGTA TACCCAACTG GGCACATTCC TCATGCTAAT CATGTGCATT 
AGCTGGGCCT TTGCCACATT TTTCTTTCAA TGCATGTGCC GCCTGCTTGG ACCCCAAGGC 
ACTTGTGGGC AAATTCCATT GCCAAAGAAG CTGCAGTGTG CAGAGCGTAC ATCTGAAAAC 
CAGTCTACTG TGAATAACCA AGGTAAAGCT GGTGCTTGCT ACACAACCAA GCATGAACAC 
TATGAGCTTG AACCCTTAGC GGCGAATGTC AGAAATGAGG GAAAGGCCTC AGATGAGGAA 
CAAGAAGCCT GCACGCAGCT GCAGAATAAC ATCACTTCTC TTCATGATGA TGCAACCTTG 
TGTGCATCCC ATGAAGAACC CAAACATGAG TTTGAAAATG GCGTGCAAGC CACAGAACAT 
TCACATCTCC ATCAGTACGC TTTCAATACA AGGTGTACCT GCGAGAACTC AATGCATCAG 
TATATTCGGG AGCGACAAAG AACTGCTTGC CATCCTTGCG GCCAATCTCG AGAAACCCAA 
TGTGCTACTA AACAGTCCAA TCACTTACCT GTGTGTCTAA ACCGGGATAT GATCCATGCT 
CCTACCAACA GTGGCCACTT CCAGCATTGC ACACCAAGCC AAATATCAAG GCCAGCTGCT 
CATCGATGTT TCAGTGGGGG CTGTGGTATG CATGCGGTGC ACATTTCACA ACAGCAAACA 
GAGACGGAGT CCTACATCAG TGCATGTGTC CCCAGTTCAT CTCCACAAGA GAGCCAGAAC 
CGTCCTGCTC ACCTCCAGTT TCCAGATGTT CAACGCTGCT CTTCGCCTGA CACGACTGGA 
GTTTGTACTC TTAATCACGA TACACAGAAC GCTGGGGAAC TGGCGATAAA TTCCCAGAGT 
CCTCAGAAAA TACCATGGAA CCATTTAAAA ACAAAAGCAA ACGGCACTTG TGTGCTCCAG 
GATTGTCGTG AGGTGGTACC TGAAGACAGC ATCGGATGCA GAGTTGATAA AGAAAAGACT 
GTCTCTCCCA AAAAGTTTAA CTGTTTTAAG AAAAACGTTA ACGCAAAGTG CAATTCTGTA 
GAGTTTCACA AGACGAGAAC CAGCGTGCCT ACTATCGCCA TCAACTCGAA AGCCTCCTCT 
GAGAGTTTAT GCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Visualization of Gli activity in craniofacial tissues of hedgehog-pathway reporter transgenic zebrafish.
PloS one5(12)e14396(2010 Dec)
Schwend T,Loucks EJ,Ahlgren SC

Zebrafish con/disp1 reveals multiple spatiotemporal requirements for Hedgehog-signaling in craniofacial development.
BMC developmental biology959(2009 Nov)
Schwend T,Ahlgren SC

Germ cell migration in zebrafish is cyclopamine-sensitive but Smoothened-independent.
Developmental biology328(2)342-54(2009 Apr)
Mich JK,Blaser H,Thomas NA,Firestone AJ,Yelon D,Raz E,Chen JK

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disp1 cDNA ORF clone, Danio rerio(zebrafish)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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