robo2 gene cDNA ORF clone, Danio rerio(zebrafish)

mRNA	Protein	Name
XM_017351010.1	XP_017206499.1	roundabout homolog 2 isoform X12
XM_021481452.1	XP_021337127.1	roundabout homolog 2 isoform X1
XM_021481460.1	XP_021337135.1	roundabout homolog 2 isoform X10
XM_017351008.1	XP_017206497.1	roundabout homolog 2 isoform X10
XM_021481453.1	XP_021337128.1	roundabout homolog 2 isoform X3
XM_021481459.1	XP_021337134.1	roundabout homolog 2 isoform X9
XM_021481461.1	XP_021337136.1	roundabout homolog 2 isoform X11
XM_017351000.1	XP_017206489.1	roundabout homolog 2 isoform X3
XM_017351009.1	XP_017206498.1	roundabout homolog 2 isoform X11
XM_021481458.1	XP_021337133.1	roundabout homolog 2 isoform X8
XM_017350998.1	XP_017206487.1	roundabout homolog 2 isoform X1
XM_017351002.1	XP_017206491.1	roundabout homolog 2 isoform X5
XM_017351003.1	XP_017206492.1	roundabout homolog 2 isoform X6
XM_017351004.1	XP_017206493.1	roundabout homolog 2 isoform X7
XM_021481455.1	XP_021337130.1	roundabout homolog 2 isoform X5
XM_021481457.1	XP_021337132.1	roundabout homolog 2 isoform X7
NM_131633.1	NP_571708.1	roundabout homolog 2 precursor
XM_017350999.1	XP_017206488.1	roundabout homolog 2 isoform X2
XM_017351001.1	XP_017206490.1	roundabout homolog 2 isoform X4
XM_017351006.1	XP_017206495.1	roundabout homolog 2 isoform X8
XM_017351007.1	XP_017206496.1	roundabout homolog 2 isoform X9
XM_017351000.2	XP_017206489.1	roundabout homolog 2 isoform X2
XM_021481454.1	XP_021337129.1	roundabout homolog 2 isoform X4
XM_021481456.1	XP_021337131.1	roundabout homolog 2 isoform X6

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa148975	XM_021481452.1 Latest version!	Danio rerio roundabout, axon guidance receptor, homolog 2 (Drosophila) (robo2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
ODa148976	XM_021481453.1 Latest version!	Danio rerio roundabout, axon guidance receptor, homolog 2 (Drosophila) (robo2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
ODa148977	XM_021481454.1 Latest version!	Danio rerio roundabout, axon guidance receptor, homolog 2 (Drosophila) (robo2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
ODa148978	XM_021481455.1 Latest version!	Danio rerio roundabout, axon guidance receptor, homolog 2 (Drosophila) (robo2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
ODa148979	XM_021481456.1 Latest version!	Danio rerio roundabout, axon guidance receptor, homolog 2 (Drosophila) (robo2), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
ODa148980	XM_021481457.1 Latest version!	Danio rerio roundabout, axon guidance receptor, homolog 2 (Drosophila) (robo2), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
ODa148981	XM_021481458.1 Latest version!	Danio rerio roundabout, axon guidance receptor, homolog 2 (Drosophila) (robo2), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
ODa148982	XM_021481459.1 Latest version!	Danio rerio roundabout, axon guidance receptor, homolog 2 (Drosophila) (robo2), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
ODa148983	XM_021481460.1 Latest version!	Danio rerio roundabout, axon guidance receptor, homolog 2 (Drosophila) (robo2), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
ODa148984	XM_021481461.1 Latest version!	Danio rerio roundabout, axon guidance receptor, homolog 2 (Drosophila) (robo2), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
ODa55117	XM_017350998.1 Latest version!	Danio rerio roundabout, axon guidance receptor, homolog 2 (Drosophila) (robo2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
ODa55118	XM_017350999.1 Latest version!	Danio rerio roundabout, axon guidance receptor, homolog 2 (Drosophila) (robo2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
ODa55119	XM_017351000.2 Latest version!	Danio rerio roundabout, axon guidance receptor, homolog 2 (Drosophila) (robo2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
ODa55120	XM_017351001.1 Latest version!	Danio rerio roundabout, axon guidance receptor, homolog 2 (Drosophila) (robo2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
ODa55121	XM_017351002.1 Latest version!	Danio rerio roundabout, axon guidance receptor, homolog 2 (Drosophila) (robo2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
ODa55122	XM_017351003.1 Latest version!	Danio rerio roundabout, axon guidance receptor, homolog 2 (Drosophila) (robo2), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
ODa55123	XM_017351004.1 Latest version!	Danio rerio roundabout, axon guidance receptor, homolog 2 (Drosophila) (robo2), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
ODa55124	XM_017351006.1 Latest version!	Danio rerio roundabout, axon guidance receptor, homolog 2 (Drosophila) (robo2), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
ODa55125	XM_017351007.1 Latest version!	Danio rerio roundabout, axon guidance receptor, homolog 2 (Drosophila) (robo2), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
ODa55126	XM_017351008.1 Latest version!	Danio rerio roundabout, axon guidance receptor, homolog 2 (Drosophila) (robo2), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
ODa55127	XM_017351009.1 Latest version!	Danio rerio roundabout, axon guidance receptor, homolog 2 (Drosophila) (robo2), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
ODa55128	XM_017351010.1 Latest version!	Danio rerio roundabout, axon guidance receptor, homolog 2 (Drosophila) (robo2), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
ODa02796	NM_131633.1 Latest version!	Danio rerio roundabout, axon guidance receptor, homolog 2 (Drosophila) (robo2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
View Old Accession Versions >>
ODa55119	XM_017351000.1	Danio rerio roundabout, axon guidance receptor, homolog 2 (Drosophila) (robo2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
Hide Old Accession Versions >>

robo2 ( XM_021481452.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021337127.1 Danio rerio roundabout, axon guidance receptor, homolog 2 (Drosophila) (robo2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa148975
Clone ID Related Accession (Same CDS sequence)	XM_021481452.1
Accession Version	XM_021481452.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4788bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	roundabout homolog 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGTCT CCCCTGAGCG AGCGAGGTGT GCTGTGGTCC TGGCTGGACC TCCTCTCCTC 
CTCATTATCT GTGTCCTGTG GATCCTGTGC TCAGACTCAG TGGATGCTCA AGGGCAAGGA 
TCCCGTCTGC GGCAGGAAGA TTCTCCACCT CGGATTGTAG AACACCCCTC CGATCTGATC 
GTGTCAAAGG GCGAGCCAGC CACCCTAAAC TGCAAGGCGG AGGGTCGGCC AACTCCCACC 
GTGGAGTGGT ACAAGGATGG AGAACGAGTT GAAACGGACA AAGATGACCC TCGATCTCAC 
CGCATGCTCC TTCCCAGTGG TTCGCTCTTC TTCCTGCGTA TCGTACATGG ACGCCGCAGC 
AAACCTGATG AGGGGGCGTA CGTCTGTGTG GCACGCAACT ACTTGGGGGA AGCAGTCAGT 
CGAAACGCCT CGCTGGAAGT AGCATTGTTA CGGGATGATT TCCGGCAGAA CCCTACAGAT 
GTGGTGGTCG CTGCTGGAGA GCCGGCCATC CTGGAGTGTG TCCCTCCCAG AGGACACCCG 
GAGCCCACCA TCTACTGGAA GAAAGACAAA GTCCGAATTG ATGAGAAGGA TGACAGGATC 
AAGATCCGTG GAGGGAAGCT GATGATCTCT AACACCAGGA AAAGCGACGC TGGAATGTAC 
ATCTGCGTGG GCACCAACAT GGTCGGCGAA AGAGACAGTG AAACAGCCCA GGTCACTGTG 
TTCGAGAGGC CCACGTTCCT GCGGCGTCCC ATTAACCAGG TGGTTCTGGA GGAGGAGGCT 
GTGGAATTTC GATGTCAGGT GCAGGGAGAC CCGCAGCCGT CCATCCGCTG GAAAAAAGAC 
GACGTTGAAA TCCCCCGCGG CAGGTACGAT ATGAAATTTG ATAAAGATGA CTTCACGCTG 
AGAATAAAGA AGGCAACAGC GAGTGATGAA GGAACCTTCA CCTGTGTAGC GGAGAACCGA 
GTGGGCAAAA TGGAGGCCTC CGCAACCCTC ACGGTCAGAG CTCGCCCTGT CGCTGCACCG 
CAGTTCGTGA TCAGGCCGCG GGATCAGATT GTGGCACAGG GACGCACCGC CAGCTTCCCC 
TGCGAGACTA AAGGGAACCC GCAGCCGGCA GTCTTCTGGC AGAAAGAAGG AAGTCAGAAT 
CTCCTGTTTC CAAATCAACC CCAGCAGCCC AACAGTCGTT TCTCGGTGTC GCCCAGTGGA 
GATCTAACCA TCACTACGGT CCAGCGGGCA GATGCGGGCT ATTATATCTG CCAGGCCCTG 
ACGGTGGCGG GCAGCATCCT GGCCAAAGCA CAGCTTGAGG TCACAGACGT GTTGACCGAT 
CGCCCTCCAC CCATCATCCG GCAGGGTCCA GCTAACCAGA CTCTTGGTGT AGACAGTGTG 
GCTCTTTTGA GGTGCCATGC ATCCGGAGAA CCAGTCCCAA CCATCAGCTG GCTGAAGGAC 
GGGGTCAGTC TGCTGGGCAA AGACCCTCGG ATGTCCCTGC AAGACCTCGG CAGCCTGCAG 
ATCAAGGGTT TACGGCTCTC TGATTCTGGC ATTTATACTT GTGTGGCTGC AAGTTCTAGT 
GGGGAAACAT CATGGAGTGC TTTCTTGGAG GTCAAAGAGG CTGGGAGTCT TGTTGTAGTT 
AAAGATCGGG ATGAGAACGA GCTTCCAGGA CCTCCTTCCA AACCTCAGGT CACAGATGTC 
ACTAAAAACA GCGTGTCATT ATCCTGGCAG CCAGGCCTTC CCGGAGCATC AGCCATTTCT 
ACATATGTCA TAGAAGCATT CAGCCAATCG GTGAGCAACA GCTGGCAAAC TGTTGCCGAT 
CACGCCAAGA CAACGCAGTA CACCATTAAG GGTCTCCGCC CTAACACCAT CTACCTGTTC 
ATGGTGCGAG CGGTCAACGT CCAAGGCCTG AGCGACCCAA GCCCCATGTC TGAACCCGTC 
CGCACTCAAG ATATAAGTCC TCCAGCGCAG GGTGTGGATC ACAGACATGT GCAAAAGGAG 
CTGGGAGAAG TTATTGTGCG GCTACACAAC CCTGTGGTCC TTAGCCCTAC CACCATACAG 
GTCACATGGA CGGTTGACCG CCAGTCTCAG TTCATCCAGG GCTACAGGGT TTTGTACAGG 
CAGATGTCAG GCCTGTCTTC TCCAGGAGCC TGGCAAACTC AGGATGTGAA AGTCCCCTCA 
GAGAGGAGCA TGGTGCTCTC GGCTCTAAAG AAAGGCATCG TCTACGAGAT CAAAGTGCGT 
CCATATTTCA ATGAGTTTCA GGGCATGGAC AGCGAATCCC GAACCGCAAG AACAACAGAG 
GAAGCGCCCA GTGCTCCTCC TCAGCAGGTG ACTGTACTCA CTGTGGGCAA CCAGAACAGC 
ACATCTATTA GCATCTCCTG GGACCCTCCG CCTGCAGAAC ACCAGAACGG CATCATACAG 
GAGTACAAGA TCTGGTGTTT GGGGAATGAG ACCCGTTTTC ATGTGAATAA GACGGTGGAC 
GCAGCGATCC GCTCAGTGGT GGTTGGGGGT CTCCAGGCGG GGGTCCAGTA TCGGGTGGAG 
GTGGCCGCTA GCACCAGCGC CGGGGTCGGA GTGAAGAGCG AACCACAAAC CATCATCATT 
GGTAAAGACT TCCGCGATGT GATGATTAGC AGGAATAACA GCATCACAGA GCAGATCACG 
GATGTGGTGA AACAGCCGGC CTTCATCGCG GGCATTGGCG GGGCCTGCTG GGTCATTCTG 
ATGGGCTTCA GCATCTGGCT CTACTGGAGG AGGAAGAAGA GAAAGGGACT CAGCAATTAC 
GCAGTTCAGT CGTTCACCTT CACCCCTGCA GTGACGTTCC AGCGTGTTGA TGGAGGCTTG 
ATGAGCAACG GGAGTCGTCC TGGTCTGCTG AACGCCAGTG ATTCGGGTTA CCCGTGGCTC 
GCCGACTCCT GGCCTCCCAG CCTGCCTGTC AACAGCGGAT CTGGAGGACC CAATGACCTC 
AGCTTTGGCC GTGGAGATGT GATGCCCAGT GCTCAGGCTG ATAAGACGGG TACCATGCTT 
TCGGATGGAG CCATTTACAG CAGCATCGAC TTCACCACCA AAGGCGGATT CGGTAGCCCA 
AGCCCAGCCT CCCAGGCCAC GCCCTATGCC ACCACACAGA TCCTCCACTC CAACAGCATC 
CATGAGCTGG CAGTGGATCT TCCTGAAGCC CAGTGGAAGA CCTCCCTACA GGCCAAGCAG 
GAGATGGCCA GCCTGGGTTA CTCACTGCCC GACAAAAACT CCTGCAATAA CACACTCCTT 
TTCATTCCTG ACTACCGATT GGCTGACGGA CTGTCTAATA GAATGCCACA CAACCAATCG 
CAGGATTTCA GCACCACCAG CTCCCACAAC AGCTCAGATC GGAGCAACAG TCTGTCAGGT 
GGAAAAGGTG GTAAGAAGAA GAAGACCAAA GCAAACGTCA AGCCCACCAA GATGAATGGC 
TCCAACTGGG CGAACGTTCC ACTTCCTCCT CCTCCCATCC ACCCTCTTCC TGGCACAGAG 
ATGGACCACT ACGCCTCAGA GCACCATGAT GGAGGAGGAT ATGATAGTGA TGGTTGGGGC 
CCACCATTGC CAGTGCAGAC GTACCTCCAT CAGGGTTTAG AGGATGAGCT GGAGGAAGAG 
GAAGAGCGGG TGCCAACTCC TCCCATTAGG GGTGTGGCTT CTTCTCCTGC TGCAGTTTCC 
TTTGGCAAAC AGTCCACAGC CACACTGAGC CCATCTCCAC GTGAAGAAAT GCAGCCCATG 
CTCCAAGCCC ACCTAGATGA GCTTACCCGT GCTTATCAAT TGGACATGGC TAAACAGACT 
TGGCACATGC AGGGTGGGCC TTACCCGCCC CAGGCCCCCG CACCACCCGT GGGCTACGTC 
TCCAGCACCT TAGTGTCTGA CATCGAGACC GATCTCCCTG ATGAAGATGA CGATGATGAG 
GAGGATGAAG GATACGAGAT GTCGCCACCA ATTAGGGGCA CTGAACACAC GCCAGGCTCC 
AGCATGGACA ACTTAGACAG CTCCGTTACG GGCTCTATGG TGAACGGCTG GGGCTCGGCT 
TCAGAAGATG ATGACAGAAA CTTCTCCAGC AGGAGGTCTA GTGCCGGCAG CTCTTCTGAC 
GGCTCGCTCT TCACCCATTG CAGCTTTGCG CGAGCCCTGG TGGCAGCCGC AGACAAGGCT 
GGTTACCGTC TGGATGGCAC CAGCCTGAGC AAAAGAGATA AAGGTCTACC TCATAGACCT 
CGTCCCGCTA GCCCGTTCTC CACAGATGGA AGCACGGTGG GCACACACAG TCTGGGGCAC 
CAGCGGGCAG CAAGGCCCAC ACGTAAACAC AAAGGCCCTG GCACTGTCCC GAACCGCCGT 
GATGCCAGTG CGGATGATCT GCCTCCACCT CCAGAGCCTC CACCCTGCCA GACAGCTCGC 
ATCCCCGGGT CGAGGAACGT TCCCCATCCC TGCGACCGCA AAGCTTCCTC TCTGGAGAGG 
CAGCCTATGA TGGGCCTTGA GGAAATGAGA AGTTCCTTGG ACCGGCAGGC CCGAACCTCA 
TTGGACCGCC ACCAACAAGT CCAGGACAGA CTGGGGTCCA TAGAGAGAGG AGATGAAAGC 
AGAAGAGGAC AGAAAAATGG TCCCTTTTCA GAAGAGGCCC TATTGCCATA CAACAAACCA 
TCATTCCCAT CCCCTGGTGG GCACAGCTCA TCTGGGACGG CCTCTTCTAA AGGGTCAACG 
GGTCCTCGCA AAGGAGAAGG GACGAGGGGA CAGCACCAGC GCTCCACCAC GGAACAAGGA 
GAAAGCAGCT ACATGGGGAC CAACGCGCAG TTTTCCGGAG AGTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021337127.1
CDS	252..5039
Translation	MAVSPERARCAVVLAGPPLLLIICVLWILCSDSVDAQGQGSRLRQEDSPPRIVEHPSDLIVSKGEPATLNCKAEGRPTPTVEWYKDGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRRSKPDEGAYVCVARNYLGEAVSRNASLEVALLRDDFRQNPTDVVVAAGEPAILECVPPRGHPEPTIYWKKDKVRIDEKDDRIKIRGGKLMISNTRKSDAGMYICVGTNMVGERDSETAQVTVFERPTFLRRPINQVVLEEEAVEFRCQVQGDPQPSIRWKKDDVEIPRGRYDMKFDKDDFTLRIKKATASDEGTFTCVAENRVGKMEASATLTVRARPVAAPQFVIRPRDQIVAQGRTASFPCETKGNPQPAVFWQKEGSQNLLFPNQPQQPNSRFSVSPSGDLTITTVQRADAGYYICQALTVAGSILAKAQLEVTDVLTDRPPPIIRQGPANQTLGVDSVALLRCHASGEPVPTISWLKDGVSLLGKDPRMSLQDLGSLQIKGLRLSDSGIYTCVAASSSGETSWSAFLEVKEAGSLVVVKDRDENELPGPPSKPQVTDVTKNSVSLSWQPGLPGASAISTYVIEAFSQSVSNSWQTVADHAKTTQYTIKGLRPNTIYLFMVRAVNVQGLSDPSPMSEPVRTQDISPPAQGVDHRHVQKELGEVIVRLHNPVVLSPTTIQVTWTVDRQSQFIQGYRVLYRQMSGLSSPGAWQTQDVKVPSERSMVLSALKKGIVYEIKVRPYFNEFQGMDSESRTARTTEEAPSAPPQQVTVLTVGNQNSTSISISWDPPPAEHQNGIIQEYKIWCLGNETRFHVNKTVDAAIRSVVVGGLQAGVQYRVEVAASTSAGVGVKSEPQTIIIGKDFRDVMISRNNSITEQITDVVKQPAFIAGIGGACWVILMGFSIWLYWRRKKRKGLSNYAVQSFTFTPAVTFQRVDGGLMSNGSRPGLLNASDSGYPWLADSWPPSLPVNSGSGGPNDLSFGRGDVMPSAQADKTGTMLSDGAIYSSIDFTTKGGFGSPSPASQATPYATTQILHSNSIHELAVDLPEAQWKTSLQAKQEMASLGYSLPDKNSCNNTLLFIPDYRLADGLSNRMPHNQSQDFSTTSSHNSSDRSNSLSGGKGGKKKKTKANVKPTKMNGSNWANVPLPPPPIHPLPGTEMDHYASEHHDGGGYDSDGWGPPLPVQTYLHQGLEDELEEEEERVPTPPIRGVASSPAAVSFGKQSTATLSPSPREEMQPMLQAHLDELTRAYQLDMAKQTWHMQGGPYPPQAPAPPVGYVSSTLVSDIETDLPDEDDDDEEDEGYEMSPPIRGTEHTPGSSMDNLDSSVTGSMVNGWGSASEDDDRNFSSRRSSAGSSSDGSLFTHCSFARALVAAADKAGYRLDGTSLSKRDKGLPHRPRPASPFSTDGSTVGTHSLGHQRAARPTRKHKGPGTVPNRRDASADDLPPPPEPPPCQTARIPGSRNVPHPCDRKASSLERQPMMGLEEMRSSLDRQARTSLDRHQQVQDRLGSIERGDESRRGQKNGPFSEEALLPYNKPSFPSPGGHSSSGTASSKGSTGPRKGEGTRGQHQRSTTEQGESSYMGTNAQFSGEL

Target ORF information:

RefSeq Version	XM_021481452.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio roundabout, axon guidance receptor, homolog 2 (Drosophila) (robo2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021481452.1

ORF Insert Sequence:

ATGGCTGTCT CCCCTGAGCG AGCGAGGTGT GCTGTGGTCC TGGCTGGACC TCCTCTCCTC 
CTCATTATCT GTGTCCTGTG GATCCTGTGC TCAGACTCAG TGGATGCTCA AGGGCAAGGA 
TCCCGTCTGC GGCAGGAAGA TTCTCCACCT CGGATTGTAG AACACCCCTC CGATCTGATC 
GTGTCAAAGG GCGAGCCAGC CACCCTAAAC TGCAAGGCGG AGGGTCGGCC AACTCCCACC 
GTGGAGTGGT ACAAGGATGG AGAACGAGTT GAAACGGACA AAGATGACCC TCGATCTCAC 
CGCATGCTCC TTCCCAGTGG TTCGCTCTTC TTCCTGCGTA TCGTACATGG ACGCCGCAGC 
AAACCTGATG AGGGGGCGTA CGTCTGTGTG GCACGCAACT ACTTGGGGGA AGCAGTCAGT 
CGAAACGCCT CGCTGGAAGT AGCATTGTTA CGGGATGATT TCCGGCAGAA CCCTACAGAT 
GTGGTGGTCG CTGCTGGAGA GCCGGCCATC CTGGAGTGTG TCCCTCCCAG AGGACACCCG 
GAGCCCACCA TCTACTGGAA GAAAGACAAA GTCCGAATTG ATGAGAAGGA TGACAGGATC 
AAGATCCGTG GAGGGAAGCT GATGATCTCT AACACCAGGA AAAGCGACGC TGGAATGTAC 
ATCTGCGTGG GCACCAACAT GGTCGGCGAA AGAGACAGTG AAACAGCCCA GGTCACTGTG 
TTCGAGAGGC CCACGTTCCT GCGGCGTCCC ATTAACCAGG TGGTTCTGGA GGAGGAGGCT 
GTGGAATTTC GATGTCAGGT GCAGGGAGAC CCGCAGCCGT CCATCCGCTG GAAAAAAGAC 
GACGTTGAAA TCCCCCGCGG CAGGTACGAT ATGAAATTTG ATAAAGATGA CTTCACGCTG 
AGAATAAAGA AGGCAACAGC GAGTGATGAA GGAACCTTCA CCTGTGTAGC GGAGAACCGA 
GTGGGCAAAA TGGAGGCCTC CGCAACCCTC ACGGTCAGAG CTCGCCCTGT CGCTGCACCG 
CAGTTCGTGA TCAGGCCGCG GGATCAGATT GTGGCACAGG GACGCACCGC CAGCTTCCCC 
TGCGAGACTA AAGGGAACCC GCAGCCGGCA GTCTTCTGGC AGAAAGAAGG AAGTCAGAAT 
CTCCTGTTTC CAAATCAACC CCAGCAGCCC AACAGTCGTT TCTCGGTGTC GCCCAGTGGA 
GATCTAACCA TCACTACGGT CCAGCGGGCA GATGCGGGCT ATTATATCTG CCAGGCCCTG 
ACGGTGGCGG GCAGCATCCT GGCCAAAGCA CAGCTTGAGG TCACAGACGT GTTGACCGAT 
CGCCCTCCAC CCATCATCCG GCAGGGTCCA GCTAACCAGA CTCTTGGTGT AGACAGTGTG 
GCTCTTTTGA GGTGCCATGC ATCCGGAGAA CCAGTCCCAA CCATCAGCTG GCTGAAGGAC 
GGGGTCAGTC TGCTGGGCAA AGACCCTCGG ATGTCCCTGC AAGACCTCGG CAGCCTGCAG 
ATCAAGGGTT TACGGCTCTC TGATTCTGGC ATTTATACTT GTGTGGCTGC AAGTTCTAGT 
GGGGAAACAT CATGGAGTGC TTTCTTGGAG GTCAAAGAGG CTGGGAGTCT TGTTGTAGTT 
AAAGATCGGG ATGAGAACGA GCTTCCAGGA CCTCCTTCCA AACCTCAGGT CACAGATGTC 
ACTAAAAACA GCGTGTCATT ATCCTGGCAG CCAGGCCTTC CCGGAGCATC AGCCATTTCT 
ACATATGTCA TAGAAGCATT CAGCCAATCG GTGAGCAACA GCTGGCAAAC TGTTGCCGAT 
CACGCCAAGA CAACGCAGTA CACCATTAAG GGTCTCCGCC CTAACACCAT CTACCTGTTC 
ATGGTGCGAG CGGTCAACGT CCAAGGCCTG AGCGACCCAA GCCCCATGTC TGAACCCGTC 
CGCACTCAAG ATATAAGTCC TCCAGCGCAG GGTGTGGATC ACAGACATGT GCAAAAGGAG 
CTGGGAGAAG TTATTGTGCG GCTACACAAC CCTGTGGTCC TTAGCCCTAC CACCATACAG 
GTCACATGGA CGGTTGACCG CCAGTCTCAG TTCATCCAGG GCTACAGGGT TTTGTACAGG 
CAGATGTCAG GCCTGTCTTC TCCAGGAGCC TGGCAAACTC AGGATGTGAA AGTCCCCTCA 
GAGAGGAGCA TGGTGCTCTC GGCTCTAAAG AAAGGCATCG TCTACGAGAT CAAAGTGCGT 
CCATATTTCA ATGAGTTTCA GGGCATGGAC AGCGAATCCC GAACCGCAAG AACAACAGAG 
GAAGCGCCCA GTGCTCCTCC TCAGCAGGTG ACTGTACTCA CTGTGGGCAA CCAGAACAGC 
ACATCTATTA GCATCTCCTG GGACCCTCCG CCTGCAGAAC ACCAGAACGG CATCATACAG 
GAGTACAAGA TCTGGTGTTT GGGGAATGAG ACCCGTTTTC ATGTGAATAA GACGGTGGAC 
GCAGCGATCC GCTCAGTGGT GGTTGGGGGT CTCCAGGCGG GGGTCCAGTA TCGGGTGGAG 
GTGGCCGCTA GCACCAGCGC CGGGGTCGGA GTGAAGAGCG AACCACAAAC CATCATCATT 
GGTAAAGACT TCCGCGATGT GATGATTAGC AGGAATAACA GCATCACAGA GCAGATCACG 
GATGTGGTGA AACAGCCGGC CTTCATCGCG GGCATTGGCG GGGCCTGCTG GGTCATTCTG 
ATGGGCTTCA GCATCTGGCT CTACTGGAGG AGGAAGAAGA GAAAGGGACT CAGCAATTAC 
GCAGTTCAGT CGTTCACCTT CACCCCTGCA GTGACGTTCC AGCGTGTTGA TGGAGGCTTG 
ATGAGCAACG GGAGTCGTCC TGGTCTGCTG AACGCCAGTG ATTCGGGTTA CCCGTGGCTC 
GCCGACTCCT GGCCTCCCAG CCTGCCTGTC AACAGCGGAT CTGGAGGACC CAATGACCTC 
AGCTTTGGCC GTGGAGATGT GATGCCCAGT GCTCAGGCTG ATAAGACGGG TACCATGCTT 
TCGGATGGAG CCATTTACAG CAGCATCGAC TTCACCACCA AAGGCGGATT CGGTAGCCCA 
AGCCCAGCCT CCCAGGCCAC GCCCTATGCC ACCACACAGA TCCTCCACTC CAACAGCATC 
CATGAGCTGG CAGTGGATCT TCCTGAAGCC CAGTGGAAGA CCTCCCTACA GGCCAAGCAG 
GAGATGGCCA GCCTGGGTTA CTCACTGCCC GACAAAAACT CCTGCAATAA CACACTCCTT 
TTCATTCCTG ACTACCGATT GGCTGACGGA CTGTCTAATA GAATGCCACA CAACCAATCG 
CAGGATTTCA GCACCACCAG CTCCCACAAC AGCTCAGATC GGAGCAACAG TCTGTCAGGT 
GGAAAAGGTG GTAAGAAGAA GAAGACCAAA GCAAACGTCA AGCCCACCAA GATGAATGGC 
TCCAACTGGG CGAACGTTCC ACTTCCTCCT CCTCCCATCC ACCCTCTTCC TGGCACAGAG 
ATGGACCACT ACGCCTCAGA GCACCATGAT GGAGGAGGAT ATGATAGTGA TGGTTGGGGC 
CCACCATTGC CAGTGCAGAC GTACCTCCAT CAGGGTTTAG AGGATGAGCT GGAGGAAGAG 
GAAGAGCGGG TGCCAACTCC TCCCATTAGG GGTGTGGCTT CTTCTCCTGC TGCAGTTTCC 
TTTGGCAAAC AGTCCACAGC CACACTGAGC CCATCTCCAC GTGAAGAAAT GCAGCCCATG 
CTCCAAGCCC ACCTAGATGA GCTTACCCGT GCTTATCAAT TGGACATGGC TAAACAGACT 
TGGCACATGC AGGGTGGGCC TTACCCGCCC CAGGCCCCCG CACCACCCGT GGGCTACGTC 
TCCAGCACCT TAGTGTCTGA CATCGAGACC GATCTCCCTG ATGAAGATGA CGATGATGAG 
GAGGATGAAG GATACGAGAT GTCGCCACCA ATTAGGGGCA CTGAACACAC GCCAGGCTCC 
AGCATGGACA ACTTAGACAG CTCCGTTACG GGCTCTATGG TGAACGGCTG GGGCTCGGCT 
TCAGAAGATG ATGACAGAAA CTTCTCCAGC AGGAGGTCTA GTGCCGGCAG CTCTTCTGAC 
GGCTCGCTCT TCACCCATTG CAGCTTTGCG CGAGCCCTGG TGGCAGCCGC AGACAAGGCT 
GGTTACCGTC TGGATGGCAC CAGCCTGAGC AAAAGAGATA AAGGTCTACC TCATAGACCT 
CGTCCCGCTA GCCCGTTCTC CACAGATGGA AGCACGGTGG GCACACACAG TCTGGGGCAC 
CAGCGGGCAG CAAGGCCCAC ACGTAAACAC AAAGGCCCTG GCACTGTCCC GAACCGCCGT 
GATGCCAGTG CGGATGATCT GCCTCCACCT CCAGAGCCTC CACCCTGCCA GACAGCTCGC 
ATCCCCGGGT CGAGGAACGT TCCCCATCCC TGCGACCGCA AAGCTTCCTC TCTGGAGAGG 
CAGCCTATGA TGGGCCTTGA GGAAATGAGA AGTTCCTTGG ACCGGCAGGC CCGAACCTCA 
TTGGACCGCC ACCAACAAGT CCAGGACAGA CTGGGGTCCA TAGAGAGAGG AGATGAAAGC 
AGAAGAGGAC AGAAAAATGG TCCCTTTTCA GAAGAGGCCC TATTGCCATA CAACAAACCA 
TCATTCCCAT CCCCTGGTGG GCACAGCTCA TCTGGGACGG CCTCTTCTAA AGGGTCAACG 
GGTCCTCGCA AAGGAGAAGG GACGAGGGGA CAGCACCAGC GCTCCACCAC GGAACAAGGA 
GAAAGCAGCT ACATGGGGAC CAACGCGCAG TTTTCCGGAG AGTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

robo2 ( XM_021481453.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021337128.1 Danio rerio roundabout, axon guidance receptor, homolog 2 (Drosophila) (robo2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa148976
Clone ID Related Accession (Same CDS sequence)	XM_021481453.1
Accession Version	XM_021481453.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4776bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	roundabout homolog 2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGTCT CCCCTGAGCG AGCGAGGTGT GCTGTGGTCC TGGCTGGACC TCCTCTCCTC 
CTCATTATCT GTGTCCTGTG GATCCTGTGC TCAGACTCAG TGGATGCTCA AGGGCAAGGA 
TCCCGTCTGC GGCAGGAAGA TTCTCCACCT CGGATTGTAG AACACCCCTC CGATCTGATC 
GTGTCAAAGG GCGAGCCAGC CACCCTAAAC TGCAAGGCGG AGGGTCGGCC AACTCCCACC 
GTGGAGTGGT ACAAGGATGG AGAACGAGTT GAAACGGACA AAGATGACCC TCGATCTCAC 
CGCATGCTCC TTCCCAGTGG TTCGCTCTTC TTCCTGCGTA TCGTACATGG ACGCCGCAGC 
AAACCTGATG AGGGGGCGTA CGTCTGTGTG GCACGCAACT ACTTGGGGGA AGCAGTCAGT 
CGAAACGCCT CGCTGGAAGT AGCATTGTTA CGGGATGATT TCCGGCAGAA CCCTACAGAT 
GTGGTGGTCG CTGCTGGAGA GCCGGCCATC CTGGAGTGTG TCCCTCCCAG AGGACACCCG 
GAGCCCACCA TCTACTGGAA GAAAGACAAA GTCCGAATTG ATGAGAAGGA TGACAGGATC 
AAGATCCGTG GAGGGAAGCT GATGATCTCT AACACCAGGA AAAGCGACGC TGGAATGTAC 
ATCTGCGTGG GCACCAACAT GGTCGGCGAA AGAGACAGTG AAACAGCCCA GGTCACTGTG 
TTCGAGAGGC CCACGTTCCT GCGGCGTCCC ATTAACCAGG TGGTTCTGGA GGAGGAGGCT 
GTGGAATTTC GATGTCAGGT GCAGGGAGAC CCGCAGCCGT CCATCCGCTG GAAAAAAGAC 
GACGTTGAAA TCCCCCGCGG CAGGTACGAT ATGAAATTTG ATAAAGATGA CTTCACGCTG 
AGAATAAAGA AGGCAACAGC GAGTGATGAA GGAACCTTCA CCTGTGTAGC GGAGAACCGA 
GTGGGCAAAA TGGAGGCCTC CGCAACCCTC ACGGTCAGAG CTGCACCGCA GTTCGTGATC 
AGGCCGCGGG ATCAGATTGT GGCACAGGGA CGCACCGCCA GCTTCCCCTG CGAGACTAAA 
GGGAACCCGC AGCCGGCAGT CTTCTGGCAG AAAGAAGGAA GTCAGAATCT CCTGTTTCCA 
AATCAACCCC AGCAGCCCAA CAGTCGTTTC TCGGTGTCGC CCAGTGGAGA TCTAACCATC 
ACTACGGTCC AGCGGGCAGA TGCGGGCTAT TATATCTGCC AGGCCCTGAC GGTGGCGGGC 
AGCATCCTGG CCAAAGCACA GCTTGAGGTC ACAGACGTGT TGACCGATCG CCCTCCACCC 
ATCATCCGGC AGGGTCCAGC TAACCAGACT CTTGGTGTAG ACAGTGTGGC TCTTTTGAGG 
TGCCATGCAT CCGGAGAACC AGTCCCAACC ATCAGCTGGC TGAAGGACGG GGTCAGTCTG 
CTGGGCAAAG ACCCTCGGAT GTCCCTGCAA GACCTCGGCA GCCTGCAGAT CAAGGGTTTA 
CGGCTCTCTG ATTCTGGCAT TTATACTTGT GTGGCTGCAA GTTCTAGTGG GGAAACATCA 
TGGAGTGCTT TCTTGGAGGT CAAAGAGGCT GGGAGTCTTG TTGTAGTTAA AGATCGGGAT 
GAGAACGAGC TTCCAGGACC TCCTTCCAAA CCTCAGGTCA CAGATGTCAC TAAAAACAGC 
GTGTCATTAT CCTGGCAGCC AGGCCTTCCC GGAGCATCAG CCATTTCTAC ATATGTCATA 
GAAGCATTCA GCCAATCGGT GAGCAACAGC TGGCAAACTG TTGCCGATCA CGCCAAGACA 
ACGCAGTACA CCATTAAGGG TCTCCGCCCT AACACCATCT ACCTGTTCAT GGTGCGAGCG 
GTCAACGTCC AAGGCCTGAG CGACCCAAGC CCCATGTCTG AACCCGTCCG CACTCAAGAT 
ATAAGTCCTC CAGCGCAGGG TGTGGATCAC AGACATGTGC AAAAGGAGCT GGGAGAAGTT 
ATTGTGCGGC TACACAACCC TGTGGTCCTT AGCCCTACCA CCATACAGGT CACATGGACG 
GTTGACCGCC AGTCTCAGTT CATCCAGGGC TACAGGGTTT TGTACAGGCA GATGTCAGGC 
CTGTCTTCTC CAGGAGCCTG GCAAACTCAG GATGTGAAAG TCCCCTCAGA GAGGAGCATG 
GTGCTCTCGG CTCTAAAGAA AGGCATCGTC TACGAGATCA AAGTGCGTCC ATATTTCAAT 
GAGTTTCAGG GCATGGACAG CGAATCCCGA ACCGCAAGAA CAACAGAGGA AGCGCCCAGT 
GCTCCTCCTC AGCAGGTGAC TGTACTCACT GTGGGCAACC AGAACAGCAC ATCTATTAGC 
ATCTCCTGGG ACCCTCCGCC TGCAGAACAC CAGAACGGCA TCATACAGGA GTACAAGATC 
TGGTGTTTGG GGAATGAGAC CCGTTTTCAT GTGAATAAGA CGGTGGACGC AGCGATCCGC 
TCAGTGGTGG TTGGGGGTCT CCAGGCGGGG GTCCAGTATC GGGTGGAGGT GGCCGCTAGC 
ACCAGCGCCG GGGTCGGAGT GAAGAGCGAA CCACAAACCA TCATCATTGG TAAAGACTTC 
CGCGATGTGA TGATTAGCAG GAATAACAGC ATCACAGAGC AGATCACGGA TGTGGTGAAA 
CAGCCGGCCT TCATCGCGGG CATTGGCGGG GCCTGCTGGG TCATTCTGAT GGGCTTCAGC 
ATCTGGCTCT ACTGGAGGAG GAAGAAGAGA AAGGGACTCA GCAATTACGC AGTTCAGTCG 
TTCACCTTCA CCCCTGCAGT GACGTTCCAG CGTGTTGATG GAGGCTTGAT GAGCAACGGG 
AGTCGTCCTG GTCTGCTGAA CGCCAGTGAT TCGGGTTACC CGTGGCTCGC CGACTCCTGG 
CCTCCCAGCC TGCCTGTCAA CAGCGGATCT GGAGGACCCA ATGACCTCAG CTTTGGCCGT 
GGAGATGTGA TGCCCAGTGC TCAGGCTGAT AAGACGGGTA CCATGCTTTC GGATGGAGCC 
ATTTACAGCA GCATCGACTT CACCACCAAA GGCGGATTCG GTAGCCCAAG CCCAGCCTCC 
CAGGCCACGC CCTATGCCAC CACACAGATC CTCCACTCCA ACAGCATCCA TGAGCTGGCA 
GTGGATCTTC CTGAAGCCCA GTGGAAGACC TCCCTACAGG CCAAGCAGGA GATGGCCAGC 
CTGGGTTACT CACTGCCCGA CAAAAACTCC TGCAATAACA CACTCCTTTT CATTCCTGAC 
TACCGATTGG CTGACGGACT GTCTAATAGA ATGCCACACA ACCAATCGCA GGATTTCAGC 
ACCACCAGCT CCCACAACAG CTCAGATCGG AGCAACAGTC TGTCAGGTGG AAAAGGTGGT 
AAGAAGAAGA AGACCAAAGC AAACGTCAAG CCCACCAAGA TGAATGGCTC CAACTGGGCG 
AACGTTCCAC TTCCTCCTCC TCCCATCCAC CCTCTTCCTG GCACAGAGAT GGACCACTAC 
GCCTCAGAGC ACCATGATGG AGGAGGATAT GATAGTGATG GTTGGGGCCC ACCATTGCCA 
GTGCAGACGT ACCTCCATCA GGGTTTAGAG GATGAGCTGG AGGAAGAGGA AGAGCGGGTG 
CCAACTCCTC CCATTAGGGG TGTGGCTTCT TCTCCTGCTG CAGTTTCCTT TGGCAAACAG 
TCCACAGCCA CACTGAGCCC ATCTCCACGT GAAGAAATGC AGCCCATGCT CCAAGCCCAC 
CTAGATGAGC TTACCCGTGC TTATCAATTG GACATGGCTA AACAGACTTG GCACATGCAG 
GGTGGGCCTT ACCCGCCCCA GGCCCCCGCA CCACCCGTGG GCTACGTCTC CAGCACCTTA 
GTGTCTGACA TCGAGACCGA TCTCCCTGAT GAAGATGACG ATGATGAGGA GGATGAAGGA 
TACGAGATGT CGCCACCAAT TAGGGGCACT GAACACACGC CAGGCTCCAG CATGGACAAC 
TTAGACAGCT CCGTTACGGG CTCTATGGTG AACGGCTGGG GCTCGGCTTC AGAAGATGAT 
GACAGAAACT TCTCCAGCAG GAGGTCTAGT GCCGGCAGCT CTTCTGACGG CTCGCTCTTC 
ACCCATTGCA GCTTTGCGCG AGCCCTGGTG GCAGCCGCAG ACAAGGCTGG TTACCGTCTG 
GATGGCACCA GCCTGAGCAA AAGAGATAAA GGTCTACCTC ATAGACCTCG TCCCGCTAGC 
CCGTTCTCCA CAGATGGAAG CACGGTGGGC ACACACAGTC TGGGGCACCA GCGGGCAGCA 
AGGCCCACAC GTAAACACAA AGGCCCTGGC ACTGTCCCGA ACCGCCGTGA TGCCAGTGCG 
GATGATCTGC CTCCACCTCC AGAGCCTCCA CCCTGCCAGA CAGCTCGCAT CCCCGGGTCG 
AGGAACGTTC CCCATCCCTG CGACCGCAAA GCTTCCTCTC TGGAGAGGCA GCCTATGATG 
GGCCTTGAGG AAATGAGAAG TTCCTTGGAC CGGCAGGCCC GAACCTCATT GGACCGCCAC 
CAACAAGTCC AGGACAGACT GGGGTCCATA GAGAGAGGAG ATGAAAGCAG AAGAGGACAG 
AAAAATGGTC CCTTTTCAGA AGAGGCCCTA TTGCCATACA ACAAACCATC ATTCCCATCC 
CCTGGTGGGC ACAGCTCATC TGGGACGGCC TCTTCTAAAG GGTCAACGGG TCCTCGCAAA 
GGAGAAGGGA CGAGGGGACA GCACCAGCGC TCCACCACGG AACAAGGAGA AAGCAGCTAC 
ATGGGGACCA ACGCGCAGTT TTCCGGAGAG TTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021337128.1
CDS	252..5027
Translation	MAVSPERARCAVVLAGPPLLLIICVLWILCSDSVDAQGQGSRLRQEDSPPRIVEHPSDLIVSKGEPATLNCKAEGRPTPTVEWYKDGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRRSKPDEGAYVCVARNYLGEAVSRNASLEVALLRDDFRQNPTDVVVAAGEPAILECVPPRGHPEPTIYWKKDKVRIDEKDDRIKIRGGKLMISNTRKSDAGMYICVGTNMVGERDSETAQVTVFERPTFLRRPINQVVLEEEAVEFRCQVQGDPQPSIRWKKDDVEIPRGRYDMKFDKDDFTLRIKKATASDEGTFTCVAENRVGKMEASATLTVRAAPQFVIRPRDQIVAQGRTASFPCETKGNPQPAVFWQKEGSQNLLFPNQPQQPNSRFSVSPSGDLTITTVQRADAGYYICQALTVAGSILAKAQLEVTDVLTDRPPPIIRQGPANQTLGVDSVALLRCHASGEPVPTISWLKDGVSLLGKDPRMSLQDLGSLQIKGLRLSDSGIYTCVAASSSGETSWSAFLEVKEAGSLVVVKDRDENELPGPPSKPQVTDVTKNSVSLSWQPGLPGASAISTYVIEAFSQSVSNSWQTVADHAKTTQYTIKGLRPNTIYLFMVRAVNVQGLSDPSPMSEPVRTQDISPPAQGVDHRHVQKELGEVIVRLHNPVVLSPTTIQVTWTVDRQSQFIQGYRVLYRQMSGLSSPGAWQTQDVKVPSERSMVLSALKKGIVYEIKVRPYFNEFQGMDSESRTARTTEEAPSAPPQQVTVLTVGNQNSTSISISWDPPPAEHQNGIIQEYKIWCLGNETRFHVNKTVDAAIRSVVVGGLQAGVQYRVEVAASTSAGVGVKSEPQTIIIGKDFRDVMISRNNSITEQITDVVKQPAFIAGIGGACWVILMGFSIWLYWRRKKRKGLSNYAVQSFTFTPAVTFQRVDGGLMSNGSRPGLLNASDSGYPWLADSWPPSLPVNSGSGGPNDLSFGRGDVMPSAQADKTGTMLSDGAIYSSIDFTTKGGFGSPSPASQATPYATTQILHSNSIHELAVDLPEAQWKTSLQAKQEMASLGYSLPDKNSCNNTLLFIPDYRLADGLSNRMPHNQSQDFSTTSSHNSSDRSNSLSGGKGGKKKKTKANVKPTKMNGSNWANVPLPPPPIHPLPGTEMDHYASEHHDGGGYDSDGWGPPLPVQTYLHQGLEDELEEEEERVPTPPIRGVASSPAAVSFGKQSTATLSPSPREEMQPMLQAHLDELTRAYQLDMAKQTWHMQGGPYPPQAPAPPVGYVSSTLVSDIETDLPDEDDDDEEDEGYEMSPPIRGTEHTPGSSMDNLDSSVTGSMVNGWGSASEDDDRNFSSRRSSAGSSSDGSLFTHCSFARALVAAADKAGYRLDGTSLSKRDKGLPHRPRPASPFSTDGSTVGTHSLGHQRAARPTRKHKGPGTVPNRRDASADDLPPPPEPPPCQTARIPGSRNVPHPCDRKASSLERQPMMGLEEMRSSLDRQARTSLDRHQQVQDRLGSIERGDESRRGQKNGPFSEEALLPYNKPSFPSPGGHSSSGTASSKGSTGPRKGEGTRGQHQRSTTEQGESSYMGTNAQFSGEL

Target ORF information:

RefSeq Version	XM_021481453.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio roundabout, axon guidance receptor, homolog 2 (Drosophila) (robo2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021481453.1

ORF Insert Sequence:

ATGGCTGTCT CCCCTGAGCG AGCGAGGTGT GCTGTGGTCC TGGCTGGACC TCCTCTCCTC 
CTCATTATCT GTGTCCTGTG GATCCTGTGC TCAGACTCAG TGGATGCTCA AGGGCAAGGA 
TCCCGTCTGC GGCAGGAAGA TTCTCCACCT CGGATTGTAG AACACCCCTC CGATCTGATC 
GTGTCAAAGG GCGAGCCAGC CACCCTAAAC TGCAAGGCGG AGGGTCGGCC AACTCCCACC 
GTGGAGTGGT ACAAGGATGG AGAACGAGTT GAAACGGACA AAGATGACCC TCGATCTCAC 
CGCATGCTCC TTCCCAGTGG TTCGCTCTTC TTCCTGCGTA TCGTACATGG ACGCCGCAGC 
AAACCTGATG AGGGGGCGTA CGTCTGTGTG GCACGCAACT ACTTGGGGGA AGCAGTCAGT 
CGAAACGCCT CGCTGGAAGT AGCATTGTTA CGGGATGATT TCCGGCAGAA CCCTACAGAT 
GTGGTGGTCG CTGCTGGAGA GCCGGCCATC CTGGAGTGTG TCCCTCCCAG AGGACACCCG 
GAGCCCACCA TCTACTGGAA GAAAGACAAA GTCCGAATTG ATGAGAAGGA TGACAGGATC 
AAGATCCGTG GAGGGAAGCT GATGATCTCT AACACCAGGA AAAGCGACGC TGGAATGTAC 
ATCTGCGTGG GCACCAACAT GGTCGGCGAA AGAGACAGTG AAACAGCCCA GGTCACTGTG 
TTCGAGAGGC CCACGTTCCT GCGGCGTCCC ATTAACCAGG TGGTTCTGGA GGAGGAGGCT 
GTGGAATTTC GATGTCAGGT GCAGGGAGAC CCGCAGCCGT CCATCCGCTG GAAAAAAGAC 
GACGTTGAAA TCCCCCGCGG CAGGTACGAT ATGAAATTTG ATAAAGATGA CTTCACGCTG 
AGAATAAAGA AGGCAACAGC GAGTGATGAA GGAACCTTCA CCTGTGTAGC GGAGAACCGA 
GTGGGCAAAA TGGAGGCCTC CGCAACCCTC ACGGTCAGAG CTGCACCGCA GTTCGTGATC 
AGGCCGCGGG ATCAGATTGT GGCACAGGGA CGCACCGCCA GCTTCCCCTG CGAGACTAAA 
GGGAACCCGC AGCCGGCAGT CTTCTGGCAG AAAGAAGGAA GTCAGAATCT CCTGTTTCCA 
AATCAACCCC AGCAGCCCAA CAGTCGTTTC TCGGTGTCGC CCAGTGGAGA TCTAACCATC 
ACTACGGTCC AGCGGGCAGA TGCGGGCTAT TATATCTGCC AGGCCCTGAC GGTGGCGGGC 
AGCATCCTGG CCAAAGCACA GCTTGAGGTC ACAGACGTGT TGACCGATCG CCCTCCACCC 
ATCATCCGGC AGGGTCCAGC TAACCAGACT CTTGGTGTAG ACAGTGTGGC TCTTTTGAGG 
TGCCATGCAT CCGGAGAACC AGTCCCAACC ATCAGCTGGC TGAAGGACGG GGTCAGTCTG 
CTGGGCAAAG ACCCTCGGAT GTCCCTGCAA GACCTCGGCA GCCTGCAGAT CAAGGGTTTA 
CGGCTCTCTG ATTCTGGCAT TTATACTTGT GTGGCTGCAA GTTCTAGTGG GGAAACATCA 
TGGAGTGCTT TCTTGGAGGT CAAAGAGGCT GGGAGTCTTG TTGTAGTTAA AGATCGGGAT 
GAGAACGAGC TTCCAGGACC TCCTTCCAAA CCTCAGGTCA CAGATGTCAC TAAAAACAGC 
GTGTCATTAT CCTGGCAGCC AGGCCTTCCC GGAGCATCAG CCATTTCTAC ATATGTCATA 
GAAGCATTCA GCCAATCGGT GAGCAACAGC TGGCAAACTG TTGCCGATCA CGCCAAGACA 
ACGCAGTACA CCATTAAGGG TCTCCGCCCT AACACCATCT ACCTGTTCAT GGTGCGAGCG 
GTCAACGTCC AAGGCCTGAG CGACCCAAGC CCCATGTCTG AACCCGTCCG CACTCAAGAT 
ATAAGTCCTC CAGCGCAGGG TGTGGATCAC AGACATGTGC AAAAGGAGCT GGGAGAAGTT 
ATTGTGCGGC TACACAACCC TGTGGTCCTT AGCCCTACCA CCATACAGGT CACATGGACG 
GTTGACCGCC AGTCTCAGTT CATCCAGGGC TACAGGGTTT TGTACAGGCA GATGTCAGGC 
CTGTCTTCTC CAGGAGCCTG GCAAACTCAG GATGTGAAAG TCCCCTCAGA GAGGAGCATG 
GTGCTCTCGG CTCTAAAGAA AGGCATCGTC TACGAGATCA AAGTGCGTCC ATATTTCAAT 
GAGTTTCAGG GCATGGACAG CGAATCCCGA ACCGCAAGAA CAACAGAGGA AGCGCCCAGT 
GCTCCTCCTC AGCAGGTGAC TGTACTCACT GTGGGCAACC AGAACAGCAC ATCTATTAGC 
ATCTCCTGGG ACCCTCCGCC TGCAGAACAC CAGAACGGCA TCATACAGGA GTACAAGATC 
TGGTGTTTGG GGAATGAGAC CCGTTTTCAT GTGAATAAGA CGGTGGACGC AGCGATCCGC 
TCAGTGGTGG TTGGGGGTCT CCAGGCGGGG GTCCAGTATC GGGTGGAGGT GGCCGCTAGC 
ACCAGCGCCG GGGTCGGAGT GAAGAGCGAA CCACAAACCA TCATCATTGG TAAAGACTTC 
CGCGATGTGA TGATTAGCAG GAATAACAGC ATCACAGAGC AGATCACGGA TGTGGTGAAA 
CAGCCGGCCT TCATCGCGGG CATTGGCGGG GCCTGCTGGG TCATTCTGAT GGGCTTCAGC 
ATCTGGCTCT ACTGGAGGAG GAAGAAGAGA AAGGGACTCA GCAATTACGC AGTTCAGTCG 
TTCACCTTCA CCCCTGCAGT GACGTTCCAG CGTGTTGATG GAGGCTTGAT GAGCAACGGG 
AGTCGTCCTG GTCTGCTGAA CGCCAGTGAT TCGGGTTACC CGTGGCTCGC CGACTCCTGG 
CCTCCCAGCC TGCCTGTCAA CAGCGGATCT GGAGGACCCA ATGACCTCAG CTTTGGCCGT 
GGAGATGTGA TGCCCAGTGC TCAGGCTGAT AAGACGGGTA CCATGCTTTC GGATGGAGCC 
ATTTACAGCA GCATCGACTT CACCACCAAA GGCGGATTCG GTAGCCCAAG CCCAGCCTCC 
CAGGCCACGC CCTATGCCAC CACACAGATC CTCCACTCCA ACAGCATCCA TGAGCTGGCA 
GTGGATCTTC CTGAAGCCCA GTGGAAGACC TCCCTACAGG CCAAGCAGGA GATGGCCAGC 
CTGGGTTACT CACTGCCCGA CAAAAACTCC TGCAATAACA CACTCCTTTT CATTCCTGAC 
TACCGATTGG CTGACGGACT GTCTAATAGA ATGCCACACA ACCAATCGCA GGATTTCAGC 
ACCACCAGCT CCCACAACAG CTCAGATCGG AGCAACAGTC TGTCAGGTGG AAAAGGTGGT 
AAGAAGAAGA AGACCAAAGC AAACGTCAAG CCCACCAAGA TGAATGGCTC CAACTGGGCG 
AACGTTCCAC TTCCTCCTCC TCCCATCCAC CCTCTTCCTG GCACAGAGAT GGACCACTAC 
GCCTCAGAGC ACCATGATGG AGGAGGATAT GATAGTGATG GTTGGGGCCC ACCATTGCCA 
GTGCAGACGT ACCTCCATCA GGGTTTAGAG GATGAGCTGG AGGAAGAGGA AGAGCGGGTG 
CCAACTCCTC CCATTAGGGG TGTGGCTTCT TCTCCTGCTG CAGTTTCCTT TGGCAAACAG 
TCCACAGCCA CACTGAGCCC ATCTCCACGT GAAGAAATGC AGCCCATGCT CCAAGCCCAC 
CTAGATGAGC TTACCCGTGC TTATCAATTG GACATGGCTA AACAGACTTG GCACATGCAG 
GGTGGGCCTT ACCCGCCCCA GGCCCCCGCA CCACCCGTGG GCTACGTCTC CAGCACCTTA 
GTGTCTGACA TCGAGACCGA TCTCCCTGAT GAAGATGACG ATGATGAGGA GGATGAAGGA 
TACGAGATGT CGCCACCAAT TAGGGGCACT GAACACACGC CAGGCTCCAG CATGGACAAC 
TTAGACAGCT CCGTTACGGG CTCTATGGTG AACGGCTGGG GCTCGGCTTC AGAAGATGAT 
GACAGAAACT TCTCCAGCAG GAGGTCTAGT GCCGGCAGCT CTTCTGACGG CTCGCTCTTC 
ACCCATTGCA GCTTTGCGCG AGCCCTGGTG GCAGCCGCAG ACAAGGCTGG TTACCGTCTG 
GATGGCACCA GCCTGAGCAA AAGAGATAAA GGTCTACCTC ATAGACCTCG TCCCGCTAGC 
CCGTTCTCCA CAGATGGAAG CACGGTGGGC ACACACAGTC TGGGGCACCA GCGGGCAGCA 
AGGCCCACAC GTAAACACAA AGGCCCTGGC ACTGTCCCGA ACCGCCGTGA TGCCAGTGCG 
GATGATCTGC CTCCACCTCC AGAGCCTCCA CCCTGCCAGA CAGCTCGCAT CCCCGGGTCG 
AGGAACGTTC CCCATCCCTG CGACCGCAAA GCTTCCTCTC TGGAGAGGCA GCCTATGATG 
GGCCTTGAGG AAATGAGAAG TTCCTTGGAC CGGCAGGCCC GAACCTCATT GGACCGCCAC 
CAACAAGTCC AGGACAGACT GGGGTCCATA GAGAGAGGAG ATGAAAGCAG AAGAGGACAG 
AAAAATGGTC CCTTTTCAGA AGAGGCCCTA TTGCCATACA ACAAACCATC ATTCCCATCC 
CCTGGTGGGC ACAGCTCATC TGGGACGGCC TCTTCTAAAG GGTCAACGGG TCCTCGCAAA 
GGAGAAGGGA CGAGGGGACA GCACCAGCGC TCCACCACGG AACAAGGAGA AAGCAGCTAC 
ATGGGGACCA ACGCGCAGTT TTCCGGAGAG TTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

robo2 ( XM_021481454.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021337129.1 Danio rerio roundabout, axon guidance receptor, homolog 2 (Drosophila) (robo2), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ODa148977
Clone ID Related Accession (Same CDS sequence)	XM_021481454.1
Accession Version	XM_021481454.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4761bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	roundabout homolog 2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGTCT CCCCTGAGCG AGCGAGGTGT GCTGTGGTCC TGGCTGGACC TCCTCTCCTC 
CTCATTATCT GTGTCCTGTG GATCCTGTGC TCAGACTCAG TGGATGCTCA AGGGCAAGGA 
TCCCGTCTGC GGCAGGAAGA TTCTCCACCT CGGATTGTAG AACACCCCTC CGATCTGATC 
GTGTCAAAGG GCGAGCCAGC CACCCTAAAC TGCAAGGCGG AGGGTCGGCC AACTCCCACC 
GTGGAGTGGT ACAAGGATGG AGAACGAGTT GAAACGGACA AAGATGACCC TCGATCTCAC 
CGCATGCTCC TTCCCAGTGG TTCGCTCTTC TTCCTGCGTA TCGTACATGG ACGCCGCAGC 
AAACCTGATG AGGGGGCGTA CGTCTGTGTG GCACGCAACT ACTTGGGGGA AGCAGTCAGT 
CGAAACGCCT CGCTGGAAGT AGCATTGTTA CGGGATGATT TCCGGCAGAA CCCTACAGAT 
GTGGTGGTCG CTGCTGGAGA GCCGGCCATC CTGGAGTGTG TCCCTCCCAG AGGACACCCG 
GAGCCCACCA TCTACTGGAA GAAAGACAAA GTCCGAATTG ATGAGAAGGA TGACAGGATC 
AAGATCCGTG GAGGGAAGCT GATGATCTCT AACACCAGGA AAAGCGACGC TGGAATGTAC 
ATCTGCGTGG GCACCAACAT GGTCGGCGAA AGAGACAGTG AAACAGCCCA GGTCACTGTG 
TTCGAGAGGC CCACGTTCCT GCGGCGTCCC ATTAACCAGG TGGTTCTGGA GGAGGAGGCT 
GTGGAATTTC GATGTCAGGT GCAGGGAGAC CCGCAGCCGT CCATCCGCTG GAAAAAAGAC 
GACGTTGAAA TCCCCCGCGG CAGGTACGAT ATGAAATTTG ATAAAGATGA CTTCACGCTG 
AGAATAAAGA AGGCAACAGC GAGTGATGAA GGAACCTTCA CCTGTGTAGC GGAGAACCGA 
GTGGGCAAAA TGGAGGCCTC CGCAACCCTC ACGGTCAGAG CTCGCCCTGT CGCTGCACCG 
CAGTTCGTGA TCAGGCCGCG GGATCAGATT GTGGCACAGG GACGCACCGC CAGCTTCCCC 
TGCGAGACTA AAGGGAACCC GCAGCCGGCA GTCTTCTGGC AGAAAGAAGG AAGTCAGAAT 
CTCCTGTTTC CAAATCAACC CCAGCAGCCC AACAGTCGTT TCTCGGTGTC GCCCAGTGGA 
GATCTAACCA TCACTACGGT CCAGCGGGCA GATGCGGGCT ATTATATCTG CCAGGCCCTG 
ACGGTGGCGG GCAGCATCCT GGCCAAAGCA CAGCTTGAGG TCACAGACGT GTTGACCGAT 
CGCCCTCCAC CCATCATCCG GCAGGGTCCA GCTAACCAGA CTCTTGGTGT AGACAGTGTG 
GCTCTTTTGA GGTGCCATGC ATCCGGAGAA CCAGTCCCAA CCATCAGCTG GCTGAAGGAC 
GGGGTCAGTC TGCTGGGCAA AGACCCTCGG ATGTCCCTGC AAGACCTCGG CAGCCTGCAG 
ATCAAGGGTT TACGGCTCTC TGATTCTGGC ATTTATACTT GTGTGGCTGC AAGTTCTAGT 
GGGGAAACAT CATGGAGTGC TTTCTTGGAG GTCAAAGAGG CTGGGAGTCT TGTTGTAGTT 
AAAGATCGGG ATGAGAACGA GCTTCCAGGA CCTCCTTCCA AACCTCAGGT CACAGATGTC 
ACTAAAAACA GCGTGTCATT ATCCTGGCAG CCAGGCCTTC CCGGAGCATC AGCCATTTCT 
ACATATGTCA TAGAAGCATT CAGCCAATCG GTGAGCAACA GCTGGCAAAC TGTTGCCGAT 
CACGCCAAGA CAACGCAGTA CACCATTAAG GGTCTCCGCC CTAACACCAT CTACCTGTTC 
ATGGTGCGAG CGGTCAACGT CCAAGGCCTG AGCGACCCAA GCCCCATGTC TGAACCCGTC 
CGCACTCAAG ATATAAGTCC TCCAGCGCAG GGTGTGGATC ACAGACATGT GCAAAAGGAG 
CTGGGAGAAG TTATTGTGCG GCTACACAAC CCTGTGGTCC TTAGCCCTAC CACCATACAG 
GTCACATGGA CGGTTGACCG CCAGTCTCAG TTCATCCAGG GCTACAGGGT TTTGTACAGG 
CAGATGTCAG GCCTGTCTTC TCCAGGAGCC TGGCAAACTC AGGATGTGAA AGTCCCCTCA 
GAGAGGAGCA TGGTGCTCTC GGCTCTAAAG AAAGGCATCG TCTACGAGAT CAAAGTGCGT 
CCATATTTCA ATGAGTTTCA GGGCATGGAC AGCGAATCCC GAACCGCAAG AACAACAGAG 
GAAGCGCCCA GTGCTCCTCC TCAGCAGGTG ACTGTACTCA CTGTGGGCAA CCAGAACAGC 
ACATCTATTA GCATCTCCTG GGACCCTCCG CCTGCAGAAC ACCAGAACGG CATCATACAG 
GAGTACAAGA TCTGGTGTTT GGGGAATGAG ACCCGTTTTC ATGTGAATAA GACGGTGGAC 
GCAGCGATCC GCTCAGTGGT GGTTGGGGGT CTCCAGGCGG GGGTCCAGTA TCGGGTGGAG 
GTGGCCGCTA GCACCAGCGC CGGGGTCGGA GTGAAGAGCG AACCACAAAC CATCATCATT 
GGTAAAGACT TCCGCGATGT GATGATTAGC AGGAATAACA GCATCACAGA GCAGATCACG 
GATGTGGTGA AACAGCCGGC CTTCATCGCG GGCATTGGCG GGGCCTGCTG GGTCATTCTG 
ATGGGCTTCA GCATCTGGCT CTACTGGAGG AGGAAGAAGA GAAAGGGACT CAGCAATTAC 
GCAGTGACGT TCCAGCGTGT TGATGGAGGC TTGATGAGCA ACGGGAGTCG TCCTGGTCTG 
CTGAACGCCA GTGATTCGGG TTACCCGTGG CTCGCCGACT CCTGGCCTCC CAGCCTGCCT 
GTCAACAGCG GATCTGGAGG ACCCAATGAC CTCAGCTTTG GCCGTGGAGA TGTGATGCCC 
AGTGCTCAGG CTGATAAGAC GGGTACCATG CTTTCGGATG GAGCCATTTA CAGCAGCATC 
GACTTCACCA CCAAAGGCGG ATTCGGTAGC CCAAGCCCAG CCTCCCAGGC CACGCCCTAT 
GCCACCACAC AGATCCTCCA CTCCAACAGC ATCCATGAGC TGGCAGTGGA TCTTCCTGAA 
GCCCAGTGGA AGACCTCCCT ACAGGCCAAG CAGGAGATGG CCAGCCTGGG TTACTCACTG 
CCCGACAAAA ACTCCTGCAA TAACACACTC CTTTTCATTC CTGACTACCG ATTGGCTGAC 
GGACTGTCTA ATAGAATGCC ACACAACCAA TCGCAGGATT TCAGCACCAC CAGCTCCCAC 
AACAGCTCAG ATCGGAGCAA CAGTCTGTCA GGTGGAAAAG GTGGTAAGAA GAAGAAGACC 
AAAGCAAACG TCAAGCCCAC CAAGATGAAT GGCTCCAACT GGGCGAACGT TCCACTTCCT 
CCTCCTCCCA TCCACCCTCT TCCTGGCACA GAGATGGACC ACTACGCCTC AGAGCACCAT 
GATGGAGGAG GATATGATAG TGATGGTTGG GGCCCACCAT TGCCAGTGCA GACGTACCTC 
CATCAGGGTT TAGAGGATGA GCTGGAGGAA GAGGAAGAGC GGGTGCCAAC TCCTCCCATT 
AGGGGTGTGG CTTCTTCTCC TGCTGCAGTT TCCTTTGGCA AACAGTCCAC AGCCACACTG 
AGCCCATCTC CACGTGAAGA AATGCAGCCC ATGCTCCAAG CCCACCTAGA TGAGCTTACC 
CGTGCTTATC AATTGGACAT GGCTAAACAG ACTTGGCACA TGCAGGGTGG GCCTTACCCG 
CCCCAGGCCC CCGCACCACC CGTGGGCTAC GTCTCCAGCA CCTTAGTGTC TGACATCGAG 
ACCGATCTCC CTGATGAAGA TGACGATGAT GAGGAGGATG AAGGATACGA GATGTCGCCA 
CCAATTAGGG GCACTGAACA CACGCCAGGC TCCAGCATGG ACAACTTAGA CAGCTCCGTT 
ACGGGCTCTA TGGTGAACGG CTGGGGCTCG GCTTCAGAAG ATGATGACAG AAACTTCTCC 
AGCAGGAGGT CTAGTGCCGG CAGCTCTTCT GACGGCTCGC TCTTCACCCA TTGCAGCTTT 
GCGCGAGCCC TGGTGGCAGC CGCAGACAAG GCTGGTTACC GTCTGGATGG CACCAGCCTG 
AGCAAAAGAG ATAAAGGTCT ACCTCATAGA CCTCGTCCCG CTAGCCCGTT CTCCACAGAT 
GGAAGCACGG TGGGCACACA CAGTCTGGGG CACCAGCGGG CAGCAAGGCC CACACGTAAA 
CACAAAGGCC CTGGCACTGT CCCGAACCGC CGTGATGCCA GTGCGGATGA TCTGCCTCCA 
CCTCCAGAGC CTCCACCCTG CCAGACAGCT CGCATCCCCG GGTCGAGGAA CGTTCCCCAT 
CCCTGCGACC GCAAAGCTTC CTCTCTGGAG AGGCAGCCTA TGATGGGCCT TGAGGAAATG 
AGAAGTTCCT TGGACCGGCA GGCCCGAACC TCATTGGACC GCCACCAACA AGTCCAGGAC 
AGACTGGGGT CCATAGAGAG AGGAGATGAA AGCAGAAGAG GACAGAAAAA TGGTCCCTTT 
TCAGAAGAGG CCCTATTGCC ATACAACAAA CCATCATTCC CATCCCCTGG TGGGCACAGC 
TCATCTGGGA CGGCCTCTTC TAAAGGGTCA ACGGGTCCTC GCAAAGGAGA AGGGACGAGG 
GGACAGCACC AGCGCTCCAC CACGGAACAA GGAGAAAGCA GCTACATGGG GACCAACGCG 
CAGTTTTCCG GAGAGTTATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021337129.1
CDS	252..5012
Translation	MAVSPERARCAVVLAGPPLLLIICVLWILCSDSVDAQGQGSRLRQEDSPPRIVEHPSDLIVSKGEPATLNCKAEGRPTPTVEWYKDGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRRSKPDEGAYVCVARNYLGEAVSRNASLEVALLRDDFRQNPTDVVVAAGEPAILECVPPRGHPEPTIYWKKDKVRIDEKDDRIKIRGGKLMISNTRKSDAGMYICVGTNMVGERDSETAQVTVFERPTFLRRPINQVVLEEEAVEFRCQVQGDPQPSIRWKKDDVEIPRGRYDMKFDKDDFTLRIKKATASDEGTFTCVAENRVGKMEASATLTVRARPVAAPQFVIRPRDQIVAQGRTASFPCETKGNPQPAVFWQKEGSQNLLFPNQPQQPNSRFSVSPSGDLTITTVQRADAGYYICQALTVAGSILAKAQLEVTDVLTDRPPPIIRQGPANQTLGVDSVALLRCHASGEPVPTISWLKDGVSLLGKDPRMSLQDLGSLQIKGLRLSDSGIYTCVAASSSGETSWSAFLEVKEAGSLVVVKDRDENELPGPPSKPQVTDVTKNSVSLSWQPGLPGASAISTYVIEAFSQSVSNSWQTVADHAKTTQYTIKGLRPNTIYLFMVRAVNVQGLSDPSPMSEPVRTQDISPPAQGVDHRHVQKELGEVIVRLHNPVVLSPTTIQVTWTVDRQSQFIQGYRVLYRQMSGLSSPGAWQTQDVKVPSERSMVLSALKKGIVYEIKVRPYFNEFQGMDSESRTARTTEEAPSAPPQQVTVLTVGNQNSTSISISWDPPPAEHQNGIIQEYKIWCLGNETRFHVNKTVDAAIRSVVVGGLQAGVQYRVEVAASTSAGVGVKSEPQTIIIGKDFRDVMISRNNSITEQITDVVKQPAFIAGIGGACWVILMGFSIWLYWRRKKRKGLSNYAVTFQRVDGGLMSNGSRPGLLNASDSGYPWLADSWPPSLPVNSGSGGPNDLSFGRGDVMPSAQADKTGTMLSDGAIYSSIDFTTKGGFGSPSPASQATPYATTQILHSNSIHELAVDLPEAQWKTSLQAKQEMASLGYSLPDKNSCNNTLLFIPDYRLADGLSNRMPHNQSQDFSTTSSHNSSDRSNSLSGGKGGKKKKTKANVKPTKMNGSNWANVPLPPPPIHPLPGTEMDHYASEHHDGGGYDSDGWGPPLPVQTYLHQGLEDELEEEEERVPTPPIRGVASSPAAVSFGKQSTATLSPSPREEMQPMLQAHLDELTRAYQLDMAKQTWHMQGGPYPPQAPAPPVGYVSSTLVSDIETDLPDEDDDDEEDEGYEMSPPIRGTEHTPGSSMDNLDSSVTGSMVNGWGSASEDDDRNFSSRRSSAGSSSDGSLFTHCSFARALVAAADKAGYRLDGTSLSKRDKGLPHRPRPASPFSTDGSTVGTHSLGHQRAARPTRKHKGPGTVPNRRDASADDLPPPPEPPPCQTARIPGSRNVPHPCDRKASSLERQPMMGLEEMRSSLDRQARTSLDRHQQVQDRLGSIERGDESRRGQKNGPFSEEALLPYNKPSFPSPGGHSSSGTASSKGSTGPRKGEGTRGQHQRSTTEQGESSYMGTNAQFSGEL

Target ORF information:

RefSeq Version	XM_021481454.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio roundabout, axon guidance receptor, homolog 2 (Drosophila) (robo2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021481454.1

ORF Insert Sequence:

ATGGCTGTCT CCCCTGAGCG AGCGAGGTGT GCTGTGGTCC TGGCTGGACC TCCTCTCCTC 
CTCATTATCT GTGTCCTGTG GATCCTGTGC TCAGACTCAG TGGATGCTCA AGGGCAAGGA 
TCCCGTCTGC GGCAGGAAGA TTCTCCACCT CGGATTGTAG AACACCCCTC CGATCTGATC 
GTGTCAAAGG GCGAGCCAGC CACCCTAAAC TGCAAGGCGG AGGGTCGGCC AACTCCCACC 
GTGGAGTGGT ACAAGGATGG AGAACGAGTT GAAACGGACA AAGATGACCC TCGATCTCAC 
CGCATGCTCC TTCCCAGTGG TTCGCTCTTC TTCCTGCGTA TCGTACATGG ACGCCGCAGC 
AAACCTGATG AGGGGGCGTA CGTCTGTGTG GCACGCAACT ACTTGGGGGA AGCAGTCAGT 
CGAAACGCCT CGCTGGAAGT AGCATTGTTA CGGGATGATT TCCGGCAGAA CCCTACAGAT 
GTGGTGGTCG CTGCTGGAGA GCCGGCCATC CTGGAGTGTG TCCCTCCCAG AGGACACCCG 
GAGCCCACCA TCTACTGGAA GAAAGACAAA GTCCGAATTG ATGAGAAGGA TGACAGGATC 
AAGATCCGTG GAGGGAAGCT GATGATCTCT AACACCAGGA AAAGCGACGC TGGAATGTAC 
ATCTGCGTGG GCACCAACAT GGTCGGCGAA AGAGACAGTG AAACAGCCCA GGTCACTGTG 
TTCGAGAGGC CCACGTTCCT GCGGCGTCCC ATTAACCAGG TGGTTCTGGA GGAGGAGGCT 
GTGGAATTTC GATGTCAGGT GCAGGGAGAC CCGCAGCCGT CCATCCGCTG GAAAAAAGAC 
GACGTTGAAA TCCCCCGCGG CAGGTACGAT ATGAAATTTG ATAAAGATGA CTTCACGCTG 
AGAATAAAGA AGGCAACAGC GAGTGATGAA GGAACCTTCA CCTGTGTAGC GGAGAACCGA 
GTGGGCAAAA TGGAGGCCTC CGCAACCCTC ACGGTCAGAG CTCGCCCTGT CGCTGCACCG 
CAGTTCGTGA TCAGGCCGCG GGATCAGATT GTGGCACAGG GACGCACCGC CAGCTTCCCC 
TGCGAGACTA AAGGGAACCC GCAGCCGGCA GTCTTCTGGC AGAAAGAAGG AAGTCAGAAT 
CTCCTGTTTC CAAATCAACC CCAGCAGCCC AACAGTCGTT TCTCGGTGTC GCCCAGTGGA 
GATCTAACCA TCACTACGGT CCAGCGGGCA GATGCGGGCT ATTATATCTG CCAGGCCCTG 
ACGGTGGCGG GCAGCATCCT GGCCAAAGCA CAGCTTGAGG TCACAGACGT GTTGACCGAT 
CGCCCTCCAC CCATCATCCG GCAGGGTCCA GCTAACCAGA CTCTTGGTGT AGACAGTGTG 
GCTCTTTTGA GGTGCCATGC ATCCGGAGAA CCAGTCCCAA CCATCAGCTG GCTGAAGGAC 
GGGGTCAGTC TGCTGGGCAA AGACCCTCGG ATGTCCCTGC AAGACCTCGG CAGCCTGCAG 
ATCAAGGGTT TACGGCTCTC TGATTCTGGC ATTTATACTT GTGTGGCTGC AAGTTCTAGT 
GGGGAAACAT CATGGAGTGC TTTCTTGGAG GTCAAAGAGG CTGGGAGTCT TGTTGTAGTT 
AAAGATCGGG ATGAGAACGA GCTTCCAGGA CCTCCTTCCA AACCTCAGGT CACAGATGTC 
ACTAAAAACA GCGTGTCATT ATCCTGGCAG CCAGGCCTTC CCGGAGCATC AGCCATTTCT 
ACATATGTCA TAGAAGCATT CAGCCAATCG GTGAGCAACA GCTGGCAAAC TGTTGCCGAT 
CACGCCAAGA CAACGCAGTA CACCATTAAG GGTCTCCGCC CTAACACCAT CTACCTGTTC 
ATGGTGCGAG CGGTCAACGT CCAAGGCCTG AGCGACCCAA GCCCCATGTC TGAACCCGTC 
CGCACTCAAG ATATAAGTCC TCCAGCGCAG GGTGTGGATC ACAGACATGT GCAAAAGGAG 
CTGGGAGAAG TTATTGTGCG GCTACACAAC CCTGTGGTCC TTAGCCCTAC CACCATACAG 
GTCACATGGA CGGTTGACCG CCAGTCTCAG TTCATCCAGG GCTACAGGGT TTTGTACAGG 
CAGATGTCAG GCCTGTCTTC TCCAGGAGCC TGGCAAACTC AGGATGTGAA AGTCCCCTCA 
GAGAGGAGCA TGGTGCTCTC GGCTCTAAAG AAAGGCATCG TCTACGAGAT CAAAGTGCGT 
CCATATTTCA ATGAGTTTCA GGGCATGGAC AGCGAATCCC GAACCGCAAG AACAACAGAG 
GAAGCGCCCA GTGCTCCTCC TCAGCAGGTG ACTGTACTCA CTGTGGGCAA CCAGAACAGC 
ACATCTATTA GCATCTCCTG GGACCCTCCG CCTGCAGAAC ACCAGAACGG CATCATACAG 
GAGTACAAGA TCTGGTGTTT GGGGAATGAG ACCCGTTTTC ATGTGAATAA GACGGTGGAC 
GCAGCGATCC GCTCAGTGGT GGTTGGGGGT CTCCAGGCGG GGGTCCAGTA TCGGGTGGAG 
GTGGCCGCTA GCACCAGCGC CGGGGTCGGA GTGAAGAGCG AACCACAAAC CATCATCATT 
GGTAAAGACT TCCGCGATGT GATGATTAGC AGGAATAACA GCATCACAGA GCAGATCACG 
GATGTGGTGA AACAGCCGGC CTTCATCGCG GGCATTGGCG GGGCCTGCTG GGTCATTCTG 
ATGGGCTTCA GCATCTGGCT CTACTGGAGG AGGAAGAAGA GAAAGGGACT CAGCAATTAC 
GCAGTGACGT TCCAGCGTGT TGATGGAGGC TTGATGAGCA ACGGGAGTCG TCCTGGTCTG 
CTGAACGCCA GTGATTCGGG TTACCCGTGG CTCGCCGACT CCTGGCCTCC CAGCCTGCCT 
GTCAACAGCG GATCTGGAGG ACCCAATGAC CTCAGCTTTG GCCGTGGAGA TGTGATGCCC 
AGTGCTCAGG CTGATAAGAC GGGTACCATG CTTTCGGATG GAGCCATTTA CAGCAGCATC 
GACTTCACCA CCAAAGGCGG ATTCGGTAGC CCAAGCCCAG CCTCCCAGGC CACGCCCTAT 
GCCACCACAC AGATCCTCCA CTCCAACAGC ATCCATGAGC TGGCAGTGGA TCTTCCTGAA 
GCCCAGTGGA AGACCTCCCT ACAGGCCAAG CAGGAGATGG CCAGCCTGGG TTACTCACTG 
CCCGACAAAA ACTCCTGCAA TAACACACTC CTTTTCATTC CTGACTACCG ATTGGCTGAC 
GGACTGTCTA ATAGAATGCC ACACAACCAA TCGCAGGATT TCAGCACCAC CAGCTCCCAC 
AACAGCTCAG ATCGGAGCAA CAGTCTGTCA GGTGGAAAAG GTGGTAAGAA GAAGAAGACC 
AAAGCAAACG TCAAGCCCAC CAAGATGAAT GGCTCCAACT GGGCGAACGT TCCACTTCCT 
CCTCCTCCCA TCCACCCTCT TCCTGGCACA GAGATGGACC ACTACGCCTC AGAGCACCAT 
GATGGAGGAG GATATGATAG TGATGGTTGG GGCCCACCAT TGCCAGTGCA GACGTACCTC 
CATCAGGGTT TAGAGGATGA GCTGGAGGAA GAGGAAGAGC GGGTGCCAAC TCCTCCCATT 
AGGGGTGTGG CTTCTTCTCC TGCTGCAGTT TCCTTTGGCA AACAGTCCAC AGCCACACTG 
AGCCCATCTC CACGTGAAGA AATGCAGCCC ATGCTCCAAG CCCACCTAGA TGAGCTTACC 
CGTGCTTATC AATTGGACAT GGCTAAACAG ACTTGGCACA TGCAGGGTGG GCCTTACCCG 
CCCCAGGCCC CCGCACCACC CGTGGGCTAC GTCTCCAGCA CCTTAGTGTC TGACATCGAG 
ACCGATCTCC CTGATGAAGA TGACGATGAT GAGGAGGATG AAGGATACGA GATGTCGCCA 
CCAATTAGGG GCACTGAACA CACGCCAGGC TCCAGCATGG ACAACTTAGA CAGCTCCGTT 
ACGGGCTCTA TGGTGAACGG CTGGGGCTCG GCTTCAGAAG ATGATGACAG AAACTTCTCC 
AGCAGGAGGT CTAGTGCCGG CAGCTCTTCT GACGGCTCGC TCTTCACCCA TTGCAGCTTT 
GCGCGAGCCC TGGTGGCAGC CGCAGACAAG GCTGGTTACC GTCTGGATGG CACCAGCCTG 
AGCAAAAGAG ATAAAGGTCT ACCTCATAGA CCTCGTCCCG CTAGCCCGTT CTCCACAGAT 
GGAAGCACGG TGGGCACACA CAGTCTGGGG CACCAGCGGG CAGCAAGGCC CACACGTAAA 
CACAAAGGCC CTGGCACTGT CCCGAACCGC CGTGATGCCA GTGCGGATGA TCTGCCTCCA 
CCTCCAGAGC CTCCACCCTG CCAGACAGCT CGCATCCCCG GGTCGAGGAA CGTTCCCCAT 
CCCTGCGACC GCAAAGCTTC CTCTCTGGAG AGGCAGCCTA TGATGGGCCT TGAGGAAATG 
AGAAGTTCCT TGGACCGGCA GGCCCGAACC TCATTGGACC GCCACCAACA AGTCCAGGAC 
AGACTGGGGT CCATAGAGAG AGGAGATGAA AGCAGAAGAG GACAGAAAAA TGGTCCCTTT 
TCAGAAGAGG CCCTATTGCC ATACAACAAA CCATCATTCC CATCCCCTGG TGGGCACAGC 
TCATCTGGGA CGGCCTCTTC TAAAGGGTCA ACGGGTCCTC GCAAAGGAGA AGGGACGAGG 
GGACAGCACC AGCGCTCCAC CACGGAACAA GGAGAAAGCA GCTACATGGG GACCAACGCG 
CAGTTTTCCG GAGAGTTATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

robo2 ( XM_021481455.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021337130.1 Danio rerio roundabout, axon guidance receptor, homolog 2 (Drosophila) (robo2), transcript variant X5, mRNA.

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CloneID	ODa148978
Clone ID Related Accession (Same CDS sequence)	XM_021481455.1
Accession Version	XM_021481455.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4728bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	roundabout homolog 2 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTCCTT TAACACACCT TTTACTCTGT GGATTAGTTT ACTTTAACCA AGTGGATGGA 
TCCCGTCTGC GGCAGGAAGA TTCTCCACCT CGGATTGTAG AACACCCCTC CGATCTGATC 
GTGTCAAAGG GCGAGCCAGC CACCCTAAAC TGCAAGGCGG AGGGTCGGCC AACTCCCACC 
GTGGAGTGGT ACAAGGATGG AGAACGAGTT GAAACGGACA AAGATGACCC TCGATCTCAC 
CGCATGCTCC TTCCCAGTGG TTCGCTCTTC TTCCTGCGTA TCGTACATGG ACGCCGCAGC 
AAACCTGATG AGGGGGCGTA CGTCTGTGTG GCACGCAACT ACTTGGGGGA AGCAGTCAGT 
CGAAACGCCT CGCTGGAAGT AGCATTGTTA CGGGATGATT TCCGGCAGAA CCCTACAGAT 
GTGGTGGTCG CTGCTGGAGA GCCGGCCATC CTGGAGTGTG TCCCTCCCAG AGGACACCCG 
GAGCCCACCA TCTACTGGAA GAAAGACAAA GTCCGAATTG ATGAGAAGGA TGACAGGATC 
AAGATCCGTG GAGGGAAGCT GATGATCTCT AACACCAGGA AAAGCGACGC TGGAATGTAC 
ATCTGCGTGG GCACCAACAT GGTCGGCGAA AGAGACAGTG AAACAGCCCA GGTCACTGTG 
TTCGAGAGGC CCACGTTCCT GCGGCGTCCC ATTAACCAGG TGGTTCTGGA GGAGGAGGCT 
GTGGAATTTC GATGTCAGGT GCAGGGAGAC CCGCAGCCGT CCATCCGCTG GAAAAAAGAC 
GACGTTGAAA TCCCCCGCGG CAGGTACGAT ATGAAATTTG ATAAAGATGA CTTCACGCTG 
AGAATAAAGA AGGCAACAGC GAGTGATGAA GGAACCTTCA CCTGTGTAGC GGAGAACCGA 
GTGGGCAAAA TGGAGGCCTC CGCAACCCTC ACGGTCAGAG CTCGCCCTGT CGCTGCACCG 
CAGTTCGTGA TCAGGCCGCG GGATCAGATT GTGGCACAGG GACGCACCGC CAGCTTCCCC 
TGCGAGACTA AAGGGAACCC GCAGCCGGCA GTCTTCTGGC AGAAAGAAGG AAGTCAGAAT 
CTCCTGTTTC CAAATCAACC CCAGCAGCCC AACAGTCGTT TCTCGGTGTC GCCCAGTGGA 
GATCTAACCA TCACTACGGT CCAGCGGGCA GATGCGGGCT ATTATATCTG CCAGGCCCTG 
ACGGTGGCGG GCAGCATCCT GGCCAAAGCA CAGCTTGAGG TCACAGACGT GTTGACCGAT 
CGCCCTCCAC CCATCATCCG GCAGGGTCCA GCTAACCAGA CTCTTGGTGT AGACAGTGTG 
GCTCTTTTGA GGTGCCATGC ATCCGGAGAA CCAGTCCCAA CCATCAGCTG GCTGAAGGAC 
GGGGTCAGTC TGCTGGGCAA AGACCCTCGG ATGTCCCTGC AAGACCTCGG CAGCCTGCAG 
ATCAAGGGTT TACGGCTCTC TGATTCTGGC ATTTATACTT GTGTGGCTGC AAGTTCTAGT 
GGGGAAACAT CATGGAGTGC TTTCTTGGAG GTCAAAGAGG CTGGGAGTCT TGTTGTAGTT 
AAAGATCGGG ATGAGAACGA GCTTCCAGGA CCTCCTTCCA AACCTCAGGT CACAGATGTC 
ACTAAAAACA GCGTGTCATT ATCCTGGCAG CCAGGCCTTC CCGGAGCATC AGCCATTTCT 
ACATATGTCA TAGAAGCATT CAGCCAATCG GTGAGCAACA GCTGGCAAAC TGTTGCCGAT 
CACGCCAAGA CAACGCAGTA CACCATTAAG GGTCTCCGCC CTAACACCAT CTACCTGTTC 
ATGGTGCGAG CGGTCAACGT CCAAGGCCTG AGCGACCCAA GCCCCATGTC TGAACCCGTC 
CGCACTCAAG ATATAAGTCC TCCAGCGCAG GGTGTGGATC ACAGACATGT GCAAAAGGAG 
CTGGGAGAAG TTATTGTGCG GCTACACAAC CCTGTGGTCC TTAGCCCTAC CACCATACAG 
GTCACATGGA CGGTTGACCG CCAGTCTCAG TTCATCCAGG GCTACAGGGT TTTGTACAGG 
CAGATGTCAG GCCTGTCTTC TCCAGGAGCC TGGCAAACTC AGGATGTGAA AGTCCCCTCA 
GAGAGGAGCA TGGTGCTCTC GGCTCTAAAG AAAGGCATCG TCTACGAGAT CAAAGTGCGT 
CCATATTTCA ATGAGTTTCA GGGCATGGAC AGCGAATCCC GAACCGCAAG AACAACAGAG 
GAAGCGCCCA GTGCTCCTCC TCAGCAGGTG ACTGTACTCA CTGTGGGCAA CCAGAACAGC 
ACATCTATTA GCATCTCCTG GGACCCTCCG CCTGCAGAAC ACCAGAACGG CATCATACAG 
GAGTACAAGA TCTGGTGTTT GGGGAATGAG ACCCGTTTTC ATGTGAATAA GACGGTGGAC 
GCAGCGATCC GCTCAGTGGT GGTTGGGGGT CTCCAGGCGG GGGTCCAGTA TCGGGTGGAG 
GTGGCCGCTA GCACCAGCGC CGGGGTCGGA GTGAAGAGCG AACCACAAAC CATCATCATT 
GGTAAAGACT TCCGCGATGT GATGATTAGC AGGAATAACA GCATCACAGA GCAGATCACG 
GATGTGGTGA AACAGCCGGC CTTCATCGCG GGCATTGGCG GGGCCTGCTG GGTCATTCTG 
ATGGGCTTCA GCATCTGGCT CTACTGGAGG AGGAAGAAGA GAAAGGGACT CAGCAATTAC 
GCAGTTCAGT CGTTCACCTT CACCCCTGCA GTGACGTTCC AGCGTGTTGA TGGAGGCTTG 
ATGAGCAACG GGAGTCGTCC TGGTCTGCTG AACGCCAGTG ATTCGGGTTA CCCGTGGCTC 
GCCGACTCCT GGCCTCCCAG CCTGCCTGTC AACAGCGGAT CTGGAGGACC CAATGACCTC 
AGCTTTGGCC GTGGAGATGT GATGCCCAGT GCTCAGGCTG ATAAGACGGG TACCATGCTT 
TCGGATGGAG CCATTTACAG CAGCATCGAC TTCACCACCA AAGGCGGATT CGGTAGCCCA 
AGCCCAGCCT CCCAGGCCAC GCCCTATGCC ACCACACAGA TCCTCCACTC CAACAGCATC 
CATGAGCTGG CAGTGGATCT TCCTGAAGCC CAGTGGAAGA CCTCCCTACA GGCCAAGCAG 
GAGATGGCCA GCCTGGGTTA CTCACTGCCC GACAAAAACT CCTGCAATAA CACACTCCTT 
TTCATTCCTG ACTACCGATT GGCTGACGGA CTGTCTAATA GAATGCCACA CAACCAATCG 
CAGGATTTCA GCACCACCAG CTCCCACAAC AGCTCAGATC GGAGCAACAG TCTGTCAGGT 
GGAAAAGGTG GTAAGAAGAA GAAGACCAAA GCAAACGTCA AGCCCACCAA GATGAATGGC 
TCCAACTGGG CGAACGTTCC ACTTCCTCCT CCTCCCATCC ACCCTCTTCC TGGCACAGAG 
ATGGACCACT ACGCCTCAGA GCACCATGAT GGAGGAGGAT ATGATAGTGA TGGTTGGGGC 
CCACCATTGC CAGTGCAGAC GTACCTCCAT CAGGGTTTAG AGGATGAGCT GGAGGAAGAG 
GAAGAGCGGG TGCCAACTCC TCCCATTAGG GGTGTGGCTT CTTCTCCTGC TGCAGTTTCC 
TTTGGCAAAC AGTCCACAGC CACACTGAGC CCATCTCCAC GTGAAGAAAT GCAGCCCATG 
CTCCAAGCCC ACCTAGATGA GCTTACCCGT GCTTATCAAT TGGACATGGC TAAACAGACT 
TGGCACATGC AGGGTGGGCC TTACCCGCCC CAGGCCCCCG CACCACCCGT GGGCTACGTC 
TCCAGCACCT TAGTGTCTGA CATCGAGACC GATCTCCCTG ATGAAGATGA CGATGATGAG 
GAGGATGAAG GATACGAGAT GTCGCCACCA ATTAGGGGCA CTGAACACAC GCCAGGCTCC 
AGCATGGACA ACTTAGACAG CTCCGTTACG GGCTCTATGG TGAACGGCTG GGGCTCGGCT 
TCAGAAGATG ATGACAGAAA CTTCTCCAGC AGGAGGTCTA GTGCCGGCAG CTCTTCTGAC 
GGCTCGCTCT TCACCCATTG CAGCTTTGCG CGAGCCCTGG TGGCAGCCGC AGACAAGGCT 
GGTTACCGTC TGGATGGCAC CAGCCTGAGC AAAAGAGATA AAGGTCTACC TCATAGACCT 
CGTCCCGCTA GCCCGTTCTC CACAGATGGA AGCACGGTGG GCACACACAG TCTGGGGCAC 
CAGCGGGCAG CAAGGCCCAC ACGTAAACAC AAAGGCCCTG GCACTGTCCC GAACCGCCGT 
GATGCCAGTG CGGATGATCT GCCTCCACCT CCAGAGCCTC CACCCTGCCA GACAGCTCGC 
ATCCCCGGGT CGAGGAACGT TCCCCATCCC TGCGACCGCA AAGCTTCCTC TCTGGAGAGG 
CAGCCTATGA TGGGCCTTGA GGAAATGAGA AGTTCCTTGG ACCGGCAGGC CCGAACCTCA 
TTGGACCGCC ACCAACAAGT CCAGGACAGA CTGGGGTCCA TAGAGAGAGG AGATGAAAGC 
AGAAGAGGAC AGAAAAATGG TCCCTTTTCA GAAGAGGCCC TATTGCCATA CAACAAACCA 
TCATTCCCAT CCCCTGGTGG GCACAGCTCA TCTGGGACGG CCTCTTCTAA AGGGTCAACG 
GGTCCTCGCA AAGGAGAAGG GACGAGGGGA CAGCACCAGC GCTCCACCAC GGAACAAGGA 
GAAAGCAGCT ACATGGGGAC CAACGCGCAG TTTTCCGGAG AGTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021337130.1
CDS	618..5345
Translation	MGPLTHLLLCGLVYFNQVDGSRLRQEDSPPRIVEHPSDLIVSKGEPATLNCKAEGRPTPTVEWYKDGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRRSKPDEGAYVCVARNYLGEAVSRNASLEVALLRDDFRQNPTDVVVAAGEPAILECVPPRGHPEPTIYWKKDKVRIDEKDDRIKIRGGKLMISNTRKSDAGMYICVGTNMVGERDSETAQVTVFERPTFLRRPINQVVLEEEAVEFRCQVQGDPQPSIRWKKDDVEIPRGRYDMKFDKDDFTLRIKKATASDEGTFTCVAENRVGKMEASATLTVRARPVAAPQFVIRPRDQIVAQGRTASFPCETKGNPQPAVFWQKEGSQNLLFPNQPQQPNSRFSVSPSGDLTITTVQRADAGYYICQALTVAGSILAKAQLEVTDVLTDRPPPIIRQGPANQTLGVDSVALLRCHASGEPVPTISWLKDGVSLLGKDPRMSLQDLGSLQIKGLRLSDSGIYTCVAASSSGETSWSAFLEVKEAGSLVVVKDRDENELPGPPSKPQVTDVTKNSVSLSWQPGLPGASAISTYVIEAFSQSVSNSWQTVADHAKTTQYTIKGLRPNTIYLFMVRAVNVQGLSDPSPMSEPVRTQDISPPAQGVDHRHVQKELGEVIVRLHNPVVLSPTTIQVTWTVDRQSQFIQGYRVLYRQMSGLSSPGAWQTQDVKVPSERSMVLSALKKGIVYEIKVRPYFNEFQGMDSESRTARTTEEAPSAPPQQVTVLTVGNQNSTSISISWDPPPAEHQNGIIQEYKIWCLGNETRFHVNKTVDAAIRSVVVGGLQAGVQYRVEVAASTSAGVGVKSEPQTIIIGKDFRDVMISRNNSITEQITDVVKQPAFIAGIGGACWVILMGFSIWLYWRRKKRKGLSNYAVQSFTFTPAVTFQRVDGGLMSNGSRPGLLNASDSGYPWLADSWPPSLPVNSGSGGPNDLSFGRGDVMPSAQADKTGTMLSDGAIYSSIDFTTKGGFGSPSPASQATPYATTQILHSNSIHELAVDLPEAQWKTSLQAKQEMASLGYSLPDKNSCNNTLLFIPDYRLADGLSNRMPHNQSQDFSTTSSHNSSDRSNSLSGGKGGKKKKTKANVKPTKMNGSNWANVPLPPPPIHPLPGTEMDHYASEHHDGGGYDSDGWGPPLPVQTYLHQGLEDELEEEEERVPTPPIRGVASSPAAVSFGKQSTATLSPSPREEMQPMLQAHLDELTRAYQLDMAKQTWHMQGGPYPPQAPAPPVGYVSSTLVSDIETDLPDEDDDDEEDEGYEMSPPIRGTEHTPGSSMDNLDSSVTGSMVNGWGSASEDDDRNFSSRRSSAGSSSDGSLFTHCSFARALVAAADKAGYRLDGTSLSKRDKGLPHRPRPASPFSTDGSTVGTHSLGHQRAARPTRKHKGPGTVPNRRDASADDLPPPPEPPPCQTARIPGSRNVPHPCDRKASSLERQPMMGLEEMRSSLDRQARTSLDRHQQVQDRLGSIERGDESRRGQKNGPFSEEALLPYNKPSFPSPGGHSSSGTASSKGSTGPRKGEGTRGQHQRSTTEQGESSYMGTNAQFSGEL

Target ORF information:

RefSeq Version	XM_021481455.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio roundabout, axon guidance receptor, homolog 2 (Drosophila) (robo2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021481455.1

ORF Insert Sequence:

ATGGGTCCTT TAACACACCT TTTACTCTGT GGATTAGTTT ACTTTAACCA AGTGGATGGA 
TCCCGTCTGC GGCAGGAAGA TTCTCCACCT CGGATTGTAG AACACCCCTC CGATCTGATC 
GTGTCAAAGG GCGAGCCAGC CACCCTAAAC TGCAAGGCGG AGGGTCGGCC AACTCCCACC 
GTGGAGTGGT ACAAGGATGG AGAACGAGTT GAAACGGACA AAGATGACCC TCGATCTCAC 
CGCATGCTCC TTCCCAGTGG TTCGCTCTTC TTCCTGCGTA TCGTACATGG ACGCCGCAGC 
AAACCTGATG AGGGGGCGTA CGTCTGTGTG GCACGCAACT ACTTGGGGGA AGCAGTCAGT 
CGAAACGCCT CGCTGGAAGT AGCATTGTTA CGGGATGATT TCCGGCAGAA CCCTACAGAT 
GTGGTGGTCG CTGCTGGAGA GCCGGCCATC CTGGAGTGTG TCCCTCCCAG AGGACACCCG 
GAGCCCACCA TCTACTGGAA GAAAGACAAA GTCCGAATTG ATGAGAAGGA TGACAGGATC 
AAGATCCGTG GAGGGAAGCT GATGATCTCT AACACCAGGA AAAGCGACGC TGGAATGTAC 
ATCTGCGTGG GCACCAACAT GGTCGGCGAA AGAGACAGTG AAACAGCCCA GGTCACTGTG 
TTCGAGAGGC CCACGTTCCT GCGGCGTCCC ATTAACCAGG TGGTTCTGGA GGAGGAGGCT 
GTGGAATTTC GATGTCAGGT GCAGGGAGAC CCGCAGCCGT CCATCCGCTG GAAAAAAGAC 
GACGTTGAAA TCCCCCGCGG CAGGTACGAT ATGAAATTTG ATAAAGATGA CTTCACGCTG 
AGAATAAAGA AGGCAACAGC GAGTGATGAA GGAACCTTCA CCTGTGTAGC GGAGAACCGA 
GTGGGCAAAA TGGAGGCCTC CGCAACCCTC ACGGTCAGAG CTCGCCCTGT CGCTGCACCG 
CAGTTCGTGA TCAGGCCGCG GGATCAGATT GTGGCACAGG GACGCACCGC CAGCTTCCCC 
TGCGAGACTA AAGGGAACCC GCAGCCGGCA GTCTTCTGGC AGAAAGAAGG AAGTCAGAAT 
CTCCTGTTTC CAAATCAACC CCAGCAGCCC AACAGTCGTT TCTCGGTGTC GCCCAGTGGA 
GATCTAACCA TCACTACGGT CCAGCGGGCA GATGCGGGCT ATTATATCTG CCAGGCCCTG 
ACGGTGGCGG GCAGCATCCT GGCCAAAGCA CAGCTTGAGG TCACAGACGT GTTGACCGAT 
CGCCCTCCAC CCATCATCCG GCAGGGTCCA GCTAACCAGA CTCTTGGTGT AGACAGTGTG 
GCTCTTTTGA GGTGCCATGC ATCCGGAGAA CCAGTCCCAA CCATCAGCTG GCTGAAGGAC 
GGGGTCAGTC TGCTGGGCAA AGACCCTCGG ATGTCCCTGC AAGACCTCGG CAGCCTGCAG 
ATCAAGGGTT TACGGCTCTC TGATTCTGGC ATTTATACTT GTGTGGCTGC AAGTTCTAGT 
GGGGAAACAT CATGGAGTGC TTTCTTGGAG GTCAAAGAGG CTGGGAGTCT TGTTGTAGTT 
AAAGATCGGG ATGAGAACGA GCTTCCAGGA CCTCCTTCCA AACCTCAGGT CACAGATGTC 
ACTAAAAACA GCGTGTCATT ATCCTGGCAG CCAGGCCTTC CCGGAGCATC AGCCATTTCT 
ACATATGTCA TAGAAGCATT CAGCCAATCG GTGAGCAACA GCTGGCAAAC TGTTGCCGAT 
CACGCCAAGA CAACGCAGTA CACCATTAAG GGTCTCCGCC CTAACACCAT CTACCTGTTC 
ATGGTGCGAG CGGTCAACGT CCAAGGCCTG AGCGACCCAA GCCCCATGTC TGAACCCGTC 
CGCACTCAAG ATATAAGTCC TCCAGCGCAG GGTGTGGATC ACAGACATGT GCAAAAGGAG 
CTGGGAGAAG TTATTGTGCG GCTACACAAC CCTGTGGTCC TTAGCCCTAC CACCATACAG 
GTCACATGGA CGGTTGACCG CCAGTCTCAG TTCATCCAGG GCTACAGGGT TTTGTACAGG 
CAGATGTCAG GCCTGTCTTC TCCAGGAGCC TGGCAAACTC AGGATGTGAA AGTCCCCTCA 
GAGAGGAGCA TGGTGCTCTC GGCTCTAAAG AAAGGCATCG TCTACGAGAT CAAAGTGCGT 
CCATATTTCA ATGAGTTTCA GGGCATGGAC AGCGAATCCC GAACCGCAAG AACAACAGAG 
GAAGCGCCCA GTGCTCCTCC TCAGCAGGTG ACTGTACTCA CTGTGGGCAA CCAGAACAGC 
ACATCTATTA GCATCTCCTG GGACCCTCCG CCTGCAGAAC ACCAGAACGG CATCATACAG 
GAGTACAAGA TCTGGTGTTT GGGGAATGAG ACCCGTTTTC ATGTGAATAA GACGGTGGAC 
GCAGCGATCC GCTCAGTGGT GGTTGGGGGT CTCCAGGCGG GGGTCCAGTA TCGGGTGGAG 
GTGGCCGCTA GCACCAGCGC CGGGGTCGGA GTGAAGAGCG AACCACAAAC CATCATCATT 
GGTAAAGACT TCCGCGATGT GATGATTAGC AGGAATAACA GCATCACAGA GCAGATCACG 
GATGTGGTGA AACAGCCGGC CTTCATCGCG GGCATTGGCG GGGCCTGCTG GGTCATTCTG 
ATGGGCTTCA GCATCTGGCT CTACTGGAGG AGGAAGAAGA GAAAGGGACT CAGCAATTAC 
GCAGTTCAGT CGTTCACCTT CACCCCTGCA GTGACGTTCC AGCGTGTTGA TGGAGGCTTG 
ATGAGCAACG GGAGTCGTCC TGGTCTGCTG AACGCCAGTG ATTCGGGTTA CCCGTGGCTC 
GCCGACTCCT GGCCTCCCAG CCTGCCTGTC AACAGCGGAT CTGGAGGACC CAATGACCTC 
AGCTTTGGCC GTGGAGATGT GATGCCCAGT GCTCAGGCTG ATAAGACGGG TACCATGCTT 
TCGGATGGAG CCATTTACAG CAGCATCGAC TTCACCACCA AAGGCGGATT CGGTAGCCCA 
AGCCCAGCCT CCCAGGCCAC GCCCTATGCC ACCACACAGA TCCTCCACTC CAACAGCATC 
CATGAGCTGG CAGTGGATCT TCCTGAAGCC CAGTGGAAGA CCTCCCTACA GGCCAAGCAG 
GAGATGGCCA GCCTGGGTTA CTCACTGCCC GACAAAAACT CCTGCAATAA CACACTCCTT 
TTCATTCCTG ACTACCGATT GGCTGACGGA CTGTCTAATA GAATGCCACA CAACCAATCG 
CAGGATTTCA GCACCACCAG CTCCCACAAC AGCTCAGATC GGAGCAACAG TCTGTCAGGT 
GGAAAAGGTG GTAAGAAGAA GAAGACCAAA GCAAACGTCA AGCCCACCAA GATGAATGGC 
TCCAACTGGG CGAACGTTCC ACTTCCTCCT CCTCCCATCC ACCCTCTTCC TGGCACAGAG 
ATGGACCACT ACGCCTCAGA GCACCATGAT GGAGGAGGAT ATGATAGTGA TGGTTGGGGC 
CCACCATTGC CAGTGCAGAC GTACCTCCAT CAGGGTTTAG AGGATGAGCT GGAGGAAGAG 
GAAGAGCGGG TGCCAACTCC TCCCATTAGG GGTGTGGCTT CTTCTCCTGC TGCAGTTTCC 
TTTGGCAAAC AGTCCACAGC CACACTGAGC CCATCTCCAC GTGAAGAAAT GCAGCCCATG 
CTCCAAGCCC ACCTAGATGA GCTTACCCGT GCTTATCAAT TGGACATGGC TAAACAGACT 
TGGCACATGC AGGGTGGGCC TTACCCGCCC CAGGCCCCCG CACCACCCGT GGGCTACGTC 
TCCAGCACCT TAGTGTCTGA CATCGAGACC GATCTCCCTG ATGAAGATGA CGATGATGAG 
GAGGATGAAG GATACGAGAT GTCGCCACCA ATTAGGGGCA CTGAACACAC GCCAGGCTCC 
AGCATGGACA ACTTAGACAG CTCCGTTACG GGCTCTATGG TGAACGGCTG GGGCTCGGCT 
TCAGAAGATG ATGACAGAAA CTTCTCCAGC AGGAGGTCTA GTGCCGGCAG CTCTTCTGAC 
GGCTCGCTCT TCACCCATTG CAGCTTTGCG CGAGCCCTGG TGGCAGCCGC AGACAAGGCT 
GGTTACCGTC TGGATGGCAC CAGCCTGAGC AAAAGAGATA AAGGTCTACC TCATAGACCT 
CGTCCCGCTA GCCCGTTCTC CACAGATGGA AGCACGGTGG GCACACACAG TCTGGGGCAC 
CAGCGGGCAG CAAGGCCCAC ACGTAAACAC AAAGGCCCTG GCACTGTCCC GAACCGCCGT 
GATGCCAGTG CGGATGATCT GCCTCCACCT CCAGAGCCTC CACCCTGCCA GACAGCTCGC 
ATCCCCGGGT CGAGGAACGT TCCCCATCCC TGCGACCGCA AAGCTTCCTC TCTGGAGAGG 
CAGCCTATGA TGGGCCTTGA GGAAATGAGA AGTTCCTTGG ACCGGCAGGC CCGAACCTCA 
TTGGACCGCC ACCAACAAGT CCAGGACAGA CTGGGGTCCA TAGAGAGAGG AGATGAAAGC 
AGAAGAGGAC AGAAAAATGG TCCCTTTTCA GAAGAGGCCC TATTGCCATA CAACAAACCA 
TCATTCCCAT CCCCTGGTGG GCACAGCTCA TCTGGGACGG CCTCTTCTAA AGGGTCAACG 
GGTCCTCGCA AAGGAGAAGG GACGAGGGGA CAGCACCAGC GCTCCACCAC GGAACAAGGA 
GAAAGCAGCT ACATGGGGAC CAACGCGCAG TTTTCCGGAG AGTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

robo2 ( XM_021481456.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021337131.1 Danio rerio roundabout, axon guidance receptor, homolog 2 (Drosophila) (robo2), transcript variant X6, mRNA.

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CloneID	ODa148979
Clone ID Related Accession (Same CDS sequence)	XM_021481456.1
Accession Version	XM_021481456.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4716bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	roundabout homolog 2 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTCCTT TAACACACCT TTTACTCTGT GGATTAGTTT ACTTTAACCA AGTGGATGGA 
TCCCGTCTGC GGCAGGAAGA TTCTCCACCT CGGATTGTAG AACACCCCTC CGATCTGATC 
GTGTCAAAGG GCGAGCCAGC CACCCTAAAC TGCAAGGCGG AGGGTCGGCC AACTCCCACC 
GTGGAGTGGT ACAAGGATGG AGAACGAGTT GAAACGGACA AAGATGACCC TCGATCTCAC 
CGCATGCTCC TTCCCAGTGG TTCGCTCTTC TTCCTGCGTA TCGTACATGG ACGCCGCAGC 
AAACCTGATG AGGGGGCGTA CGTCTGTGTG GCACGCAACT ACTTGGGGGA AGCAGTCAGT 
CGAAACGCCT CGCTGGAAGT AGCATTGTTA CGGGATGATT TCCGGCAGAA CCCTACAGAT 
GTGGTGGTCG CTGCTGGAGA GCCGGCCATC CTGGAGTGTG TCCCTCCCAG AGGACACCCG 
GAGCCCACCA TCTACTGGAA GAAAGACAAA GTCCGAATTG ATGAGAAGGA TGACAGGATC 
AAGATCCGTG GAGGGAAGCT GATGATCTCT AACACCAGGA AAAGCGACGC TGGAATGTAC 
ATCTGCGTGG GCACCAACAT GGTCGGCGAA AGAGACAGTG AAACAGCCCA GGTCACTGTG 
TTCGAGAGGC CCACGTTCCT GCGGCGTCCC ATTAACCAGG TGGTTCTGGA GGAGGAGGCT 
GTGGAATTTC GATGTCAGGT GCAGGGAGAC CCGCAGCCGT CCATCCGCTG GAAAAAAGAC 
GACGTTGAAA TCCCCCGCGG CAGGTACGAT ATGAAATTTG ATAAAGATGA CTTCACGCTG 
AGAATAAAGA AGGCAACAGC GAGTGATGAA GGAACCTTCA CCTGTGTAGC GGAGAACCGA 
GTGGGCAAAA TGGAGGCCTC CGCAACCCTC ACGGTCAGAG CTGCACCGCA GTTCGTGATC 
AGGCCGCGGG ATCAGATTGT GGCACAGGGA CGCACCGCCA GCTTCCCCTG CGAGACTAAA 
GGGAACCCGC AGCCGGCAGT CTTCTGGCAG AAAGAAGGAA GTCAGAATCT CCTGTTTCCA 
AATCAACCCC AGCAGCCCAA CAGTCGTTTC TCGGTGTCGC CCAGTGGAGA TCTAACCATC 
ACTACGGTCC AGCGGGCAGA TGCGGGCTAT TATATCTGCC AGGCCCTGAC GGTGGCGGGC 
AGCATCCTGG CCAAAGCACA GCTTGAGGTC ACAGACGTGT TGACCGATCG CCCTCCACCC 
ATCATCCGGC AGGGTCCAGC TAACCAGACT CTTGGTGTAG ACAGTGTGGC TCTTTTGAGG 
TGCCATGCAT CCGGAGAACC AGTCCCAACC ATCAGCTGGC TGAAGGACGG GGTCAGTCTG 
CTGGGCAAAG ACCCTCGGAT GTCCCTGCAA GACCTCGGCA GCCTGCAGAT CAAGGGTTTA 
CGGCTCTCTG ATTCTGGCAT TTATACTTGT GTGGCTGCAA GTTCTAGTGG GGAAACATCA 
TGGAGTGCTT TCTTGGAGGT CAAAGAGGCT GGGAGTCTTG TTGTAGTTAA AGATCGGGAT 
GAGAACGAGC TTCCAGGACC TCCTTCCAAA CCTCAGGTCA CAGATGTCAC TAAAAACAGC 
GTGTCATTAT CCTGGCAGCC AGGCCTTCCC GGAGCATCAG CCATTTCTAC ATATGTCATA 
GAAGCATTCA GCCAATCGGT GAGCAACAGC TGGCAAACTG TTGCCGATCA CGCCAAGACA 
ACGCAGTACA CCATTAAGGG TCTCCGCCCT AACACCATCT ACCTGTTCAT GGTGCGAGCG 
GTCAACGTCC AAGGCCTGAG CGACCCAAGC CCCATGTCTG AACCCGTCCG CACTCAAGAT 
ATAAGTCCTC CAGCGCAGGG TGTGGATCAC AGACATGTGC AAAAGGAGCT GGGAGAAGTT 
ATTGTGCGGC TACACAACCC TGTGGTCCTT AGCCCTACCA CCATACAGGT CACATGGACG 
GTTGACCGCC AGTCTCAGTT CATCCAGGGC TACAGGGTTT TGTACAGGCA GATGTCAGGC 
CTGTCTTCTC CAGGAGCCTG GCAAACTCAG GATGTGAAAG TCCCCTCAGA GAGGAGCATG 
GTGCTCTCGG CTCTAAAGAA AGGCATCGTC TACGAGATCA AAGTGCGTCC ATATTTCAAT 
GAGTTTCAGG GCATGGACAG CGAATCCCGA ACCGCAAGAA CAACAGAGGA AGCGCCCAGT 
GCTCCTCCTC AGCAGGTGAC TGTACTCACT GTGGGCAACC AGAACAGCAC ATCTATTAGC 
ATCTCCTGGG ACCCTCCGCC TGCAGAACAC CAGAACGGCA TCATACAGGA GTACAAGATC 
TGGTGTTTGG GGAATGAGAC CCGTTTTCAT GTGAATAAGA CGGTGGACGC AGCGATCCGC 
TCAGTGGTGG TTGGGGGTCT CCAGGCGGGG GTCCAGTATC GGGTGGAGGT GGCCGCTAGC 
ACCAGCGCCG GGGTCGGAGT GAAGAGCGAA CCACAAACCA TCATCATTGG TAAAGACTTC 
CGCGATGTGA TGATTAGCAG GAATAACAGC ATCACAGAGC AGATCACGGA TGTGGTGAAA 
CAGCCGGCCT TCATCGCGGG CATTGGCGGG GCCTGCTGGG TCATTCTGAT GGGCTTCAGC 
ATCTGGCTCT ACTGGAGGAG GAAGAAGAGA AAGGGACTCA GCAATTACGC AGTTCAGTCG 
TTCACCTTCA CCCCTGCAGT GACGTTCCAG CGTGTTGATG GAGGCTTGAT GAGCAACGGG 
AGTCGTCCTG GTCTGCTGAA CGCCAGTGAT TCGGGTTACC CGTGGCTCGC CGACTCCTGG 
CCTCCCAGCC TGCCTGTCAA CAGCGGATCT GGAGGACCCA ATGACCTCAG CTTTGGCCGT 
GGAGATGTGA TGCCCAGTGC TCAGGCTGAT AAGACGGGTA CCATGCTTTC GGATGGAGCC 
ATTTACAGCA GCATCGACTT CACCACCAAA GGCGGATTCG GTAGCCCAAG CCCAGCCTCC 
CAGGCCACGC CCTATGCCAC CACACAGATC CTCCACTCCA ACAGCATCCA TGAGCTGGCA 
GTGGATCTTC CTGAAGCCCA GTGGAAGACC TCCCTACAGG CCAAGCAGGA GATGGCCAGC 
CTGGGTTACT CACTGCCCGA CAAAAACTCC TGCAATAACA CACTCCTTTT CATTCCTGAC 
TACCGATTGG CTGACGGACT GTCTAATAGA ATGCCACACA ACCAATCGCA GGATTTCAGC 
ACCACCAGCT CCCACAACAG CTCAGATCGG AGCAACAGTC TGTCAGGTGG AAAAGGTGGT 
AAGAAGAAGA AGACCAAAGC AAACGTCAAG CCCACCAAGA TGAATGGCTC CAACTGGGCG 
AACGTTCCAC TTCCTCCTCC TCCCATCCAC CCTCTTCCTG GCACAGAGAT GGACCACTAC 
GCCTCAGAGC ACCATGATGG AGGAGGATAT GATAGTGATG GTTGGGGCCC ACCATTGCCA 
GTGCAGACGT ACCTCCATCA GGGTTTAGAG GATGAGCTGG AGGAAGAGGA AGAGCGGGTG 
CCAACTCCTC CCATTAGGGG TGTGGCTTCT TCTCCTGCTG CAGTTTCCTT TGGCAAACAG 
TCCACAGCCA CACTGAGCCC ATCTCCACGT GAAGAAATGC AGCCCATGCT CCAAGCCCAC 
CTAGATGAGC TTACCCGTGC TTATCAATTG GACATGGCTA AACAGACTTG GCACATGCAG 
GGTGGGCCTT ACCCGCCCCA GGCCCCCGCA CCACCCGTGG GCTACGTCTC CAGCACCTTA 
GTGTCTGACA TCGAGACCGA TCTCCCTGAT GAAGATGACG ATGATGAGGA GGATGAAGGA 
TACGAGATGT CGCCACCAAT TAGGGGCACT GAACACACGC CAGGCTCCAG CATGGACAAC 
TTAGACAGCT CCGTTACGGG CTCTATGGTG AACGGCTGGG GCTCGGCTTC AGAAGATGAT 
GACAGAAACT TCTCCAGCAG GAGGTCTAGT GCCGGCAGCT CTTCTGACGG CTCGCTCTTC 
ACCCATTGCA GCTTTGCGCG AGCCCTGGTG GCAGCCGCAG ACAAGGCTGG TTACCGTCTG 
GATGGCACCA GCCTGAGCAA AAGAGATAAA GGTCTACCTC ATAGACCTCG TCCCGCTAGC 
CCGTTCTCCA CAGATGGAAG CACGGTGGGC ACACACAGTC TGGGGCACCA GCGGGCAGCA 
AGGCCCACAC GTAAACACAA AGGCCCTGGC ACTGTCCCGA ACCGCCGTGA TGCCAGTGCG 
GATGATCTGC CTCCACCTCC AGAGCCTCCA CCCTGCCAGA CAGCTCGCAT CCCCGGGTCG 
AGGAACGTTC CCCATCCCTG CGACCGCAAA GCTTCCTCTC TGGAGAGGCA GCCTATGATG 
GGCCTTGAGG AAATGAGAAG TTCCTTGGAC CGGCAGGCCC GAACCTCATT GGACCGCCAC 
CAACAAGTCC AGGACAGACT GGGGTCCATA GAGAGAGGAG ATGAAAGCAG AAGAGGACAG 
AAAAATGGTC CCTTTTCAGA AGAGGCCCTA TTGCCATACA ACAAACCATC ATTCCCATCC 
CCTGGTGGGC ACAGCTCATC TGGGACGGCC TCTTCTAAAG GGTCAACGGG TCCTCGCAAA 
GGAGAAGGGA CGAGGGGACA GCACCAGCGC TCCACCACGG AACAAGGAGA AAGCAGCTAC 
ATGGGGACCA ACGCGCAGTT TTCCGGAGAG TTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021337131.1
CDS	618..5333
Translation	MGPLTHLLLCGLVYFNQVDGSRLRQEDSPPRIVEHPSDLIVSKGEPATLNCKAEGRPTPTVEWYKDGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRRSKPDEGAYVCVARNYLGEAVSRNASLEVALLRDDFRQNPTDVVVAAGEPAILECVPPRGHPEPTIYWKKDKVRIDEKDDRIKIRGGKLMISNTRKSDAGMYICVGTNMVGERDSETAQVTVFERPTFLRRPINQVVLEEEAVEFRCQVQGDPQPSIRWKKDDVEIPRGRYDMKFDKDDFTLRIKKATASDEGTFTCVAENRVGKMEASATLTVRAAPQFVIRPRDQIVAQGRTASFPCETKGNPQPAVFWQKEGSQNLLFPNQPQQPNSRFSVSPSGDLTITTVQRADAGYYICQALTVAGSILAKAQLEVTDVLTDRPPPIIRQGPANQTLGVDSVALLRCHASGEPVPTISWLKDGVSLLGKDPRMSLQDLGSLQIKGLRLSDSGIYTCVAASSSGETSWSAFLEVKEAGSLVVVKDRDENELPGPPSKPQVTDVTKNSVSLSWQPGLPGASAISTYVIEAFSQSVSNSWQTVADHAKTTQYTIKGLRPNTIYLFMVRAVNVQGLSDPSPMSEPVRTQDISPPAQGVDHRHVQKELGEVIVRLHNPVVLSPTTIQVTWTVDRQSQFIQGYRVLYRQMSGLSSPGAWQTQDVKVPSERSMVLSALKKGIVYEIKVRPYFNEFQGMDSESRTARTTEEAPSAPPQQVTVLTVGNQNSTSISISWDPPPAEHQNGIIQEYKIWCLGNETRFHVNKTVDAAIRSVVVGGLQAGVQYRVEVAASTSAGVGVKSEPQTIIIGKDFRDVMISRNNSITEQITDVVKQPAFIAGIGGACWVILMGFSIWLYWRRKKRKGLSNYAVQSFTFTPAVTFQRVDGGLMSNGSRPGLLNASDSGYPWLADSWPPSLPVNSGSGGPNDLSFGRGDVMPSAQADKTGTMLSDGAIYSSIDFTTKGGFGSPSPASQATPYATTQILHSNSIHELAVDLPEAQWKTSLQAKQEMASLGYSLPDKNSCNNTLLFIPDYRLADGLSNRMPHNQSQDFSTTSSHNSSDRSNSLSGGKGGKKKKTKANVKPTKMNGSNWANVPLPPPPIHPLPGTEMDHYASEHHDGGGYDSDGWGPPLPVQTYLHQGLEDELEEEEERVPTPPIRGVASSPAAVSFGKQSTATLSPSPREEMQPMLQAHLDELTRAYQLDMAKQTWHMQGGPYPPQAPAPPVGYVSSTLVSDIETDLPDEDDDDEEDEGYEMSPPIRGTEHTPGSSMDNLDSSVTGSMVNGWGSASEDDDRNFSSRRSSAGSSSDGSLFTHCSFARALVAAADKAGYRLDGTSLSKRDKGLPHRPRPASPFSTDGSTVGTHSLGHQRAARPTRKHKGPGTVPNRRDASADDLPPPPEPPPCQTARIPGSRNVPHPCDRKASSLERQPMMGLEEMRSSLDRQARTSLDRHQQVQDRLGSIERGDESRRGQKNGPFSEEALLPYNKPSFPSPGGHSSSGTASSKGSTGPRKGEGTRGQHQRSTTEQGESSYMGTNAQFSGEL

Target ORF information:

RefSeq Version	XM_021481456.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio roundabout, axon guidance receptor, homolog 2 (Drosophila) (robo2), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021481456.1

ORF Insert Sequence:

ATGGGTCCTT TAACACACCT TTTACTCTGT GGATTAGTTT ACTTTAACCA AGTGGATGGA 
TCCCGTCTGC GGCAGGAAGA TTCTCCACCT CGGATTGTAG AACACCCCTC CGATCTGATC 
GTGTCAAAGG GCGAGCCAGC CACCCTAAAC TGCAAGGCGG AGGGTCGGCC AACTCCCACC 
GTGGAGTGGT ACAAGGATGG AGAACGAGTT GAAACGGACA AAGATGACCC TCGATCTCAC 
CGCATGCTCC TTCCCAGTGG TTCGCTCTTC TTCCTGCGTA TCGTACATGG ACGCCGCAGC 
AAACCTGATG AGGGGGCGTA CGTCTGTGTG GCACGCAACT ACTTGGGGGA AGCAGTCAGT 
CGAAACGCCT CGCTGGAAGT AGCATTGTTA CGGGATGATT TCCGGCAGAA CCCTACAGAT 
GTGGTGGTCG CTGCTGGAGA GCCGGCCATC CTGGAGTGTG TCCCTCCCAG AGGACACCCG 
GAGCCCACCA TCTACTGGAA GAAAGACAAA GTCCGAATTG ATGAGAAGGA TGACAGGATC 
AAGATCCGTG GAGGGAAGCT GATGATCTCT AACACCAGGA AAAGCGACGC TGGAATGTAC 
ATCTGCGTGG GCACCAACAT GGTCGGCGAA AGAGACAGTG AAACAGCCCA GGTCACTGTG 
TTCGAGAGGC CCACGTTCCT GCGGCGTCCC ATTAACCAGG TGGTTCTGGA GGAGGAGGCT 
GTGGAATTTC GATGTCAGGT GCAGGGAGAC CCGCAGCCGT CCATCCGCTG GAAAAAAGAC 
GACGTTGAAA TCCCCCGCGG CAGGTACGAT ATGAAATTTG ATAAAGATGA CTTCACGCTG 
AGAATAAAGA AGGCAACAGC GAGTGATGAA GGAACCTTCA CCTGTGTAGC GGAGAACCGA 
GTGGGCAAAA TGGAGGCCTC CGCAACCCTC ACGGTCAGAG CTGCACCGCA GTTCGTGATC 
AGGCCGCGGG ATCAGATTGT GGCACAGGGA CGCACCGCCA GCTTCCCCTG CGAGACTAAA 
GGGAACCCGC AGCCGGCAGT CTTCTGGCAG AAAGAAGGAA GTCAGAATCT CCTGTTTCCA 
AATCAACCCC AGCAGCCCAA CAGTCGTTTC TCGGTGTCGC CCAGTGGAGA TCTAACCATC 
ACTACGGTCC AGCGGGCAGA TGCGGGCTAT TATATCTGCC AGGCCCTGAC GGTGGCGGGC 
AGCATCCTGG CCAAAGCACA GCTTGAGGTC ACAGACGTGT TGACCGATCG CCCTCCACCC 
ATCATCCGGC AGGGTCCAGC TAACCAGACT CTTGGTGTAG ACAGTGTGGC TCTTTTGAGG 
TGCCATGCAT CCGGAGAACC AGTCCCAACC ATCAGCTGGC TGAAGGACGG GGTCAGTCTG 
CTGGGCAAAG ACCCTCGGAT GTCCCTGCAA GACCTCGGCA GCCTGCAGAT CAAGGGTTTA 
CGGCTCTCTG ATTCTGGCAT TTATACTTGT GTGGCTGCAA GTTCTAGTGG GGAAACATCA 
TGGAGTGCTT TCTTGGAGGT CAAAGAGGCT GGGAGTCTTG TTGTAGTTAA AGATCGGGAT 
GAGAACGAGC TTCCAGGACC TCCTTCCAAA CCTCAGGTCA CAGATGTCAC TAAAAACAGC 
GTGTCATTAT CCTGGCAGCC AGGCCTTCCC GGAGCATCAG CCATTTCTAC ATATGTCATA 
GAAGCATTCA GCCAATCGGT GAGCAACAGC TGGCAAACTG TTGCCGATCA CGCCAAGACA 
ACGCAGTACA CCATTAAGGG TCTCCGCCCT AACACCATCT ACCTGTTCAT GGTGCGAGCG 
GTCAACGTCC AAGGCCTGAG CGACCCAAGC CCCATGTCTG AACCCGTCCG CACTCAAGAT 
ATAAGTCCTC CAGCGCAGGG TGTGGATCAC AGACATGTGC AAAAGGAGCT GGGAGAAGTT 
ATTGTGCGGC TACACAACCC TGTGGTCCTT AGCCCTACCA CCATACAGGT CACATGGACG 
GTTGACCGCC AGTCTCAGTT CATCCAGGGC TACAGGGTTT TGTACAGGCA GATGTCAGGC 
CTGTCTTCTC CAGGAGCCTG GCAAACTCAG GATGTGAAAG TCCCCTCAGA GAGGAGCATG 
GTGCTCTCGG CTCTAAAGAA AGGCATCGTC TACGAGATCA AAGTGCGTCC ATATTTCAAT 
GAGTTTCAGG GCATGGACAG CGAATCCCGA ACCGCAAGAA CAACAGAGGA AGCGCCCAGT 
GCTCCTCCTC AGCAGGTGAC TGTACTCACT GTGGGCAACC AGAACAGCAC ATCTATTAGC 
ATCTCCTGGG ACCCTCCGCC TGCAGAACAC CAGAACGGCA TCATACAGGA GTACAAGATC 
TGGTGTTTGG GGAATGAGAC CCGTTTTCAT GTGAATAAGA CGGTGGACGC AGCGATCCGC 
TCAGTGGTGG TTGGGGGTCT CCAGGCGGGG GTCCAGTATC GGGTGGAGGT GGCCGCTAGC 
ACCAGCGCCG GGGTCGGAGT GAAGAGCGAA CCACAAACCA TCATCATTGG TAAAGACTTC 
CGCGATGTGA TGATTAGCAG GAATAACAGC ATCACAGAGC AGATCACGGA TGTGGTGAAA 
CAGCCGGCCT TCATCGCGGG CATTGGCGGG GCCTGCTGGG TCATTCTGAT GGGCTTCAGC 
ATCTGGCTCT ACTGGAGGAG GAAGAAGAGA AAGGGACTCA GCAATTACGC AGTTCAGTCG 
TTCACCTTCA CCCCTGCAGT GACGTTCCAG CGTGTTGATG GAGGCTTGAT GAGCAACGGG 
AGTCGTCCTG GTCTGCTGAA CGCCAGTGAT TCGGGTTACC CGTGGCTCGC CGACTCCTGG 
CCTCCCAGCC TGCCTGTCAA CAGCGGATCT GGAGGACCCA ATGACCTCAG CTTTGGCCGT 
GGAGATGTGA TGCCCAGTGC TCAGGCTGAT AAGACGGGTA CCATGCTTTC GGATGGAGCC 
ATTTACAGCA GCATCGACTT CACCACCAAA GGCGGATTCG GTAGCCCAAG CCCAGCCTCC 
CAGGCCACGC CCTATGCCAC CACACAGATC CTCCACTCCA ACAGCATCCA TGAGCTGGCA 
GTGGATCTTC CTGAAGCCCA GTGGAAGACC TCCCTACAGG CCAAGCAGGA GATGGCCAGC 
CTGGGTTACT CACTGCCCGA CAAAAACTCC TGCAATAACA CACTCCTTTT CATTCCTGAC 
TACCGATTGG CTGACGGACT GTCTAATAGA ATGCCACACA ACCAATCGCA GGATTTCAGC 
ACCACCAGCT CCCACAACAG CTCAGATCGG AGCAACAGTC TGTCAGGTGG AAAAGGTGGT 
AAGAAGAAGA AGACCAAAGC AAACGTCAAG CCCACCAAGA TGAATGGCTC CAACTGGGCG 
AACGTTCCAC TTCCTCCTCC TCCCATCCAC CCTCTTCCTG GCACAGAGAT GGACCACTAC 
GCCTCAGAGC ACCATGATGG AGGAGGATAT GATAGTGATG GTTGGGGCCC ACCATTGCCA 
GTGCAGACGT ACCTCCATCA GGGTTTAGAG GATGAGCTGG AGGAAGAGGA AGAGCGGGTG 
CCAACTCCTC CCATTAGGGG TGTGGCTTCT TCTCCTGCTG CAGTTTCCTT TGGCAAACAG 
TCCACAGCCA CACTGAGCCC ATCTCCACGT GAAGAAATGC AGCCCATGCT CCAAGCCCAC 
CTAGATGAGC TTACCCGTGC TTATCAATTG GACATGGCTA AACAGACTTG GCACATGCAG 
GGTGGGCCTT ACCCGCCCCA GGCCCCCGCA CCACCCGTGG GCTACGTCTC CAGCACCTTA 
GTGTCTGACA TCGAGACCGA TCTCCCTGAT GAAGATGACG ATGATGAGGA GGATGAAGGA 
TACGAGATGT CGCCACCAAT TAGGGGCACT GAACACACGC CAGGCTCCAG CATGGACAAC 
TTAGACAGCT CCGTTACGGG CTCTATGGTG AACGGCTGGG GCTCGGCTTC AGAAGATGAT 
GACAGAAACT TCTCCAGCAG GAGGTCTAGT GCCGGCAGCT CTTCTGACGG CTCGCTCTTC 
ACCCATTGCA GCTTTGCGCG AGCCCTGGTG GCAGCCGCAG ACAAGGCTGG TTACCGTCTG 
GATGGCACCA GCCTGAGCAA AAGAGATAAA GGTCTACCTC ATAGACCTCG TCCCGCTAGC 
CCGTTCTCCA CAGATGGAAG CACGGTGGGC ACACACAGTC TGGGGCACCA GCGGGCAGCA 
AGGCCCACAC GTAAACACAA AGGCCCTGGC ACTGTCCCGA ACCGCCGTGA TGCCAGTGCG 
GATGATCTGC CTCCACCTCC AGAGCCTCCA CCCTGCCAGA CAGCTCGCAT CCCCGGGTCG 
AGGAACGTTC CCCATCCCTG CGACCGCAAA GCTTCCTCTC TGGAGAGGCA GCCTATGATG 
GGCCTTGAGG AAATGAGAAG TTCCTTGGAC CGGCAGGCCC GAACCTCATT GGACCGCCAC 
CAACAAGTCC AGGACAGACT GGGGTCCATA GAGAGAGGAG ATGAAAGCAG AAGAGGACAG 
AAAAATGGTC CCTTTTCAGA AGAGGCCCTA TTGCCATACA ACAAACCATC ATTCCCATCC 
CCTGGTGGGC ACAGCTCATC TGGGACGGCC TCTTCTAAAG GGTCAACGGG TCCTCGCAAA 
GGAGAAGGGA CGAGGGGACA GCACCAGCGC TCCACCACGG AACAAGGAGA AAGCAGCTAC 
ATGGGGACCA ACGCGCAGTT TTCCGGAGAG TTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

robo2 ( XM_021481457.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021337132.1 Danio rerio roundabout, axon guidance receptor, homolog 2 (Drosophila) (robo2), transcript variant X7, mRNA.

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CloneID	ODa148980
Clone ID Related Accession (Same CDS sequence)	XM_021481457.1
Accession Version	XM_021481457.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4662bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	roundabout homolog 2 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGTCT CCCCTGAGCG AGCGAGGTGT GCTGTGGTCC TGGCTGGACC TCCTCTCCTC 
CTCATTATCT GTGTCCTGTG GATCCTGTGC TCAGACTCAG TGGATGCTCA AGGGCAAGGA 
TCCCGTCTGC GGCAGGAAGA TTCTCCACCT CGGATTGTAG AACACCCCTC CGATCTGATC 
GTGTCAAAGG GCGAGCCAGC CACCCTAAAC TGCAAGGCGG AGGGTCGGCC AACTCCCACC 
GTGGAGTGGT ACAAGGATGG AGAACGAGTT GAAACGGACA AAGATGACCC TCGATCTCAC 
CGCATGCTCC TTCCCAGTGG TTCGCTCTTC TTCCTGCGTA TCGTACATGG ACGCCGCAGC 
AAACCTGATG AGGGGGCGTA CGTCTGTGTG GCACGCAACT ACTTGGGGGA AGCAGTCAGT 
CGAAACGCCT CGCTGGAAGT AGCATTGTTA CGGGATGATT TCCGGCAGAA CCCTACAGAT 
GTGGTGGTCG CTGCTGGAGA GCCGGCCATC CTGGAGTGTG TCCCTCCCAG AGGACACCCG 
GAGCCCACCA TCTACTGGAA GAAAGACAAA GTCCGAATTG ATGAGAAGGA TGACAGGATC 
AAGATCCGTG GAGGGAAGCT GATGATCTCT AACACCAGGA AAAGCGACGC TGGAATGTAC 
ATCTGCGTGG GCACCAACAT GGTCGGCGAA AGAGACAGTG AAACAGCCCA GGTCACTGTG 
TTCGAGAGGC CCACGTTCCT GCGGCGTCCC ATTAACCAGG TGGTTCTGGA GGAGGAGGCT 
GTGGAATTTC GATGTCAGGT GCAGGGAGAC CCGCAGCCGT CCATCCGCTG GAAAAAAGAC 
GACGTTGAAA TCCCCCGCGG CAGGTACGAT ATGAAATTTG ATAAAGATGA CTTCACGCTG 
AGAATAAAGA AGGCAACAGC GAGTGATGAA GGAACCTTCA CCTGTGTAGC GGAGAACCGA 
GTGGGCAAAA TGGAGGCCTC CGCAACCCTC ACGGTCAGAG CTCGCCCTGT CGCTGCACCG 
CAGTTCGTGA TCAGGCCGCG GGATCAGATT GTGGCACAGG GACGCACCGC CAGCTTCCCC 
TGCGAGACTA AAGGGAACCC GCAGCCGGCA GTCTTCTGGC AGAAAGAAGG AAGTCAGAAT 
CTCCTGTTTC CAAATCAACC CCAGCAGCCC AACAGTCGTT TCTCGGTGTC GCCCAGTGGA 
GATCTAACCA TCACTACGGT CCAGCGGGCA GATGCGGGCT ATTATATCTG CCAGGCCCTG 
ACGGTGGCGG GCAGCATCCT GGCCAAAGCA CAGCTTGAGG TCACAGACGT GTTGACCGAT 
CGCCCTCCAC CCATCATCCG GCAGGGTCCA GCTAACCAGA CTCTTGGTGT AGACAGTGTG 
GCTCTTTTGA GGTGCCATGC ATCCGGAGAA CCAGTCCCAA CCATCAGCTG GCTGAAGGAC 
GGGGTCAGTC TGCTGGGCAA AGACCCTCGG ATGTCCCTGC AAGACCTCGG CAGCCTGCAG 
ATCAAGGGTT TACGGCTCTC TGATTCTGGC ATTTATACTT GTGTGGCTGC AAGTTCTAGT 
GGGGAAACAT CATGGAGTGC TTTCTTGGAG GTCAAAGAGG CTGGGAGTCT TGTTGTAGTT 
AAAGATCGGG ATGAGAACGA GCTTCCAGGA CCTCCTTCCA AACCTCAGGT CACAGATGTC 
ACTAAAAACA GCGTGTCATT ATCCTGGCAG CCAGGCCTTC CCGGAGCATC AGCCATTTCT 
ACATATGTCA TAGAAGCATT CAGCCAATCG GTGAGCAACA GCTGGCAAAC TGTTGCCGAT 
CACGCCAAGA CAACGCAGTA CACCATTAAG GGTCTCCGCC CTAACACCAT CTACCTGTTC 
ATGGTGCGAG CGGTCAACGT CCAAGGCCTG AGCGACCCAA GCCCCATGTC TGAACCCGTC 
CGCACTCAAG ATATAAGTCC TCCAGCGCAG GGTGTGGATC ACAGACATGT GCAAAAGGAG 
CTGGGAGAAG TTATTGTGCG GCTACACAAC CCTGTGGTCC TTAGCCCTAC CACCATACAG 
GTCACATGGA CGGTTGACCG CCAGTCTCAG TTCATCCAGG GCTACAGGGT TTTGTACAGG 
CAGATGTCAG GCCTGTCTTC TCCAGGAGCC TGGCAAACTC AGGATGTGAA AGTCCCCTCA 
GAGAGGAGCA TGGTGCTCTC GGCTCTAAAG AAAGGCATCG TCTACGAGAT CAAAGTGCGT 
CCATATTTCA ATGAGTTTCA GGGCATGGAC AGCGAATCCC GAACCGCAAG AACAACAGAG 
GAAGCGCCCA GTGCTCCTCC TCAGCAGGTG ACTGTACTCA CTGTGGGCAA CCAGAACAGC 
ACATCTATTA GCATCTCCTG GGACCCTCCG CCTGCAGAAC ACCAGAACGG CATCATACAG 
GAGTACAAGA TCTGGTGTTT GGGGAATGAG ACCCGTTTTC ATGTGAATAA GACGGTGGAC 
GCAGCGATCC GCTCAGTGGT GGTTGGGGGT CTCCAGGCGG GGGTCCAGTA TCGGGTGGAG 
GTGGCCGCTA GCACCAGCGC CGGGGTCGGA GTGAAGAGCG AACCACAAAC CATCATCATT 
GGTAAAGACT TCCGCGATGT GATGATTAGC AGGAATAACA GCATCACAGA GCAGATCACG 
GATGTGGTGA AACAGCCGGC CTTCATCGCG GGCATTGGCG GGGCCTGCTG GGTCATTCTG 
ATGGGCTTCA GCATCTGGCT CTACTGGAGG AGGAAGAAGA GAAAGGGACT CAGCAATTAC 
GCAGTTCAGT CGTTCACCTT CACCCCTGCA GTGACGTTCC AGCGTGTTGA TGGAGGCTTG 
ATGAGCAACG GGAGTCGTCC TGGTCTGCTG AACGCCAGTG ATTCGGGTTA CCCGTGGCTC 
GCCGACTCCT GGCCTCCCAG CCTGCCTGTC AACAGCGGAT CTGGAGGACC CAATGACCTC 
AGCTTTGGCC GTGGAGATGT GATGCCCAGT GCTCAGGCTG ATAAGACGGG TACCATGCTT 
TCGGATGGAG CCATTTACAG CAGCATCGAC TTCACCACCA AAGGCGGATT CGGTAGCCCA 
AGCCCAGCCT CCCAGGCCAC GCCCTATGCC ACCACACAGA TCCTCCACTC CAACAGCATC 
CATGAGCTGG CAGTGGATCT TCCTGAAGCC CAGTGGAAGA CCTCCCTACA GGCCAAGCAG 
GAGATGGCCA GCCTGGGTTA CTCACTGCCC GACAAAAACT CCTGCAATAA CAGTGGAAAA 
GGTGGTAAGA AGAAGAAGAC CAAAGCAAAC GTCAAGCCCA CCAAGATGAA TGGCTCCAAC 
TGGGCGAACG TTCCACTTCC TCCTCCTCCC ATCCACCCTC TTCCTGGCAC AGAGATGGAC 
CACTACGCCT CAGAGCACCA TGATGGAGGA GGATATGATA GTGATGGTTG GGGCCCACCA 
TTGCCAGTGC AGACGTACCT CCATCAGGGT TTAGAGGATG AGCTGGAGGA AGAGGAAGAG 
CGGGTGCCAA CTCCTCCCAT TAGGGGTGTG GCTTCTTCTC CTGCTGCAGT TTCCTTTGGC 
AAACAGTCCA CAGCCACACT GAGCCCATCT CCACGTGAAG AAATGCAGCC CATGCTCCAA 
GCCCACCTAG ATGAGCTTAC CCGTGCTTAT CAATTGGACA TGGCTAAACA GACTTGGCAC 
ATGCAGGGTG GGCCTTACCC GCCCCAGGCC CCCGCACCAC CCGTGGGCTA CGTCTCCAGC 
ACCTTAGTGT CTGACATCGA GACCGATCTC CCTGATGAAG ATGACGATGA TGAGGAGGAT 
GAAGGATACG AGATGTCGCC ACCAATTAGG GGCACTGAAC ACACGCCAGG CTCCAGCATG 
GACAACTTAG ACAGCTCCGT TACGGGCTCT ATGGTGAACG GCTGGGGCTC GGCTTCAGAA 
GATGATGACA GAAACTTCTC CAGCAGGAGG TCTAGTGCCG GCAGCTCTTC TGACGGCTCG 
CTCTTCACCC ATTGCAGCTT TGCGCGAGCC CTGGTGGCAG CCGCAGACAA GGCTGGTTAC 
CGTCTGGATG GCACCAGCCT GAGCAAAAGA GATAAAGGTC TACCTCATAG ACCTCGTCCC 
GCTAGCCCGT TCTCCACAGA TGGAAGCACG GTGGGCACAC ACAGTCTGGG GCACCAGCGG 
GCAGCAAGGC CCACACGTAA ACACAAAGGC CCTGGCACTG TCCCGAACCG CCGTGATGCC 
AGTGCGGATG ATCTGCCTCC ACCTCCAGAG CCTCCACCCT GCCAGACAGC TCGCATCCCC 
GGGTCGAGGA ACGTTCCCCA TCCCTGCGAC CGCAAAGCTT CCTCTCTGGA GAGGCAGCCT 
ATGATGGGCC TTGAGGAAAT GAGAAGTTCC TTGGACCGGC AGGCCCGAAC CTCATTGGAC 
CGCCACCAAC AAGTCCAGGA CAGACTGGGG TCCATAGAGA GAGGAGATGA AAGCAGAAGA 
GGACAGAAAA ATGGTCCCTT TTCAGAAGAG GCCCTATTGC CATACAACAA ACCATCATTC 
CCATCCCCTG GTGGGCACAG CTCATCTGGG ACGGCCTCTT CTAAAGGGTC AACGGGTCCT 
CGCAAAGGAG AAGGGACGAG GGGACAGCAC CAGCGCTCCA CCACGGAACA AGGAGAAAGC 
AGCTACATGG GGACCAACGC GCAGTTTTCC GGAGAGTTAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021337132.1
CDS	252..4913
Translation	MAVSPERARCAVVLAGPPLLLIICVLWILCSDSVDAQGQGSRLRQEDSPPRIVEHPSDLIVSKGEPATLNCKAEGRPTPTVEWYKDGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRRSKPDEGAYVCVARNYLGEAVSRNASLEVALLRDDFRQNPTDVVVAAGEPAILECVPPRGHPEPTIYWKKDKVRIDEKDDRIKIRGGKLMISNTRKSDAGMYICVGTNMVGERDSETAQVTVFERPTFLRRPINQVVLEEEAVEFRCQVQGDPQPSIRWKKDDVEIPRGRYDMKFDKDDFTLRIKKATASDEGTFTCVAENRVGKMEASATLTVRARPVAAPQFVIRPRDQIVAQGRTASFPCETKGNPQPAVFWQKEGSQNLLFPNQPQQPNSRFSVSPSGDLTITTVQRADAGYYICQALTVAGSILAKAQLEVTDVLTDRPPPIIRQGPANQTLGVDSVALLRCHASGEPVPTISWLKDGVSLLGKDPRMSLQDLGSLQIKGLRLSDSGIYTCVAASSSGETSWSAFLEVKEAGSLVVVKDRDENELPGPPSKPQVTDVTKNSVSLSWQPGLPGASAISTYVIEAFSQSVSNSWQTVADHAKTTQYTIKGLRPNTIYLFMVRAVNVQGLSDPSPMSEPVRTQDISPPAQGVDHRHVQKELGEVIVRLHNPVVLSPTTIQVTWTVDRQSQFIQGYRVLYRQMSGLSSPGAWQTQDVKVPSERSMVLSALKKGIVYEIKVRPYFNEFQGMDSESRTARTTEEAPSAPPQQVTVLTVGNQNSTSISISWDPPPAEHQNGIIQEYKIWCLGNETRFHVNKTVDAAIRSVVVGGLQAGVQYRVEVAASTSAGVGVKSEPQTIIIGKDFRDVMISRNNSITEQITDVVKQPAFIAGIGGACWVILMGFSIWLYWRRKKRKGLSNYAVQSFTFTPAVTFQRVDGGLMSNGSRPGLLNASDSGYPWLADSWPPSLPVNSGSGGPNDLSFGRGDVMPSAQADKTGTMLSDGAIYSSIDFTTKGGFGSPSPASQATPYATTQILHSNSIHELAVDLPEAQWKTSLQAKQEMASLGYSLPDKNSCNNSGKGGKKKKTKANVKPTKMNGSNWANVPLPPPPIHPLPGTEMDHYASEHHDGGGYDSDGWGPPLPVQTYLHQGLEDELEEEEERVPTPPIRGVASSPAAVSFGKQSTATLSPSPREEMQPMLQAHLDELTRAYQLDMAKQTWHMQGGPYPPQAPAPPVGYVSSTLVSDIETDLPDEDDDDEEDEGYEMSPPIRGTEHTPGSSMDNLDSSVTGSMVNGWGSASEDDDRNFSSRRSSAGSSSDGSLFTHCSFARALVAAADKAGYRLDGTSLSKRDKGLPHRPRPASPFSTDGSTVGTHSLGHQRAARPTRKHKGPGTVPNRRDASADDLPPPPEPPPCQTARIPGSRNVPHPCDRKASSLERQPMMGLEEMRSSLDRQARTSLDRHQQVQDRLGSIERGDESRRGQKNGPFSEEALLPYNKPSFPSPGGHSSSGTASSKGSTGPRKGEGTRGQHQRSTTEQGESSYMGTNAQFSGEL

Target ORF information:

RefSeq Version	XM_021481457.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio roundabout, axon guidance receptor, homolog 2 (Drosophila) (robo2), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021481457.1

ORF Insert Sequence:

ATGGCTGTCT CCCCTGAGCG AGCGAGGTGT GCTGTGGTCC TGGCTGGACC TCCTCTCCTC 
CTCATTATCT GTGTCCTGTG GATCCTGTGC TCAGACTCAG TGGATGCTCA AGGGCAAGGA 
TCCCGTCTGC GGCAGGAAGA TTCTCCACCT CGGATTGTAG AACACCCCTC CGATCTGATC 
GTGTCAAAGG GCGAGCCAGC CACCCTAAAC TGCAAGGCGG AGGGTCGGCC AACTCCCACC 
GTGGAGTGGT ACAAGGATGG AGAACGAGTT GAAACGGACA AAGATGACCC TCGATCTCAC 
CGCATGCTCC TTCCCAGTGG TTCGCTCTTC TTCCTGCGTA TCGTACATGG ACGCCGCAGC 
AAACCTGATG AGGGGGCGTA CGTCTGTGTG GCACGCAACT ACTTGGGGGA AGCAGTCAGT 
CGAAACGCCT CGCTGGAAGT AGCATTGTTA CGGGATGATT TCCGGCAGAA CCCTACAGAT 
GTGGTGGTCG CTGCTGGAGA GCCGGCCATC CTGGAGTGTG TCCCTCCCAG AGGACACCCG 
GAGCCCACCA TCTACTGGAA GAAAGACAAA GTCCGAATTG ATGAGAAGGA TGACAGGATC 
AAGATCCGTG GAGGGAAGCT GATGATCTCT AACACCAGGA AAAGCGACGC TGGAATGTAC 
ATCTGCGTGG GCACCAACAT GGTCGGCGAA AGAGACAGTG AAACAGCCCA GGTCACTGTG 
TTCGAGAGGC CCACGTTCCT GCGGCGTCCC ATTAACCAGG TGGTTCTGGA GGAGGAGGCT 
GTGGAATTTC GATGTCAGGT GCAGGGAGAC CCGCAGCCGT CCATCCGCTG GAAAAAAGAC 
GACGTTGAAA TCCCCCGCGG CAGGTACGAT ATGAAATTTG ATAAAGATGA CTTCACGCTG 
AGAATAAAGA AGGCAACAGC GAGTGATGAA GGAACCTTCA CCTGTGTAGC GGAGAACCGA 
GTGGGCAAAA TGGAGGCCTC CGCAACCCTC ACGGTCAGAG CTCGCCCTGT CGCTGCACCG 
CAGTTCGTGA TCAGGCCGCG GGATCAGATT GTGGCACAGG GACGCACCGC CAGCTTCCCC 
TGCGAGACTA AAGGGAACCC GCAGCCGGCA GTCTTCTGGC AGAAAGAAGG AAGTCAGAAT 
CTCCTGTTTC CAAATCAACC CCAGCAGCCC AACAGTCGTT TCTCGGTGTC GCCCAGTGGA 
GATCTAACCA TCACTACGGT CCAGCGGGCA GATGCGGGCT ATTATATCTG CCAGGCCCTG 
ACGGTGGCGG GCAGCATCCT GGCCAAAGCA CAGCTTGAGG TCACAGACGT GTTGACCGAT 
CGCCCTCCAC CCATCATCCG GCAGGGTCCA GCTAACCAGA CTCTTGGTGT AGACAGTGTG 
GCTCTTTTGA GGTGCCATGC ATCCGGAGAA CCAGTCCCAA CCATCAGCTG GCTGAAGGAC 
GGGGTCAGTC TGCTGGGCAA AGACCCTCGG ATGTCCCTGC AAGACCTCGG CAGCCTGCAG 
ATCAAGGGTT TACGGCTCTC TGATTCTGGC ATTTATACTT GTGTGGCTGC AAGTTCTAGT 
GGGGAAACAT CATGGAGTGC TTTCTTGGAG GTCAAAGAGG CTGGGAGTCT TGTTGTAGTT 
AAAGATCGGG ATGAGAACGA GCTTCCAGGA CCTCCTTCCA AACCTCAGGT CACAGATGTC 
ACTAAAAACA GCGTGTCATT ATCCTGGCAG CCAGGCCTTC CCGGAGCATC AGCCATTTCT 
ACATATGTCA TAGAAGCATT CAGCCAATCG GTGAGCAACA GCTGGCAAAC TGTTGCCGAT 
CACGCCAAGA CAACGCAGTA CACCATTAAG GGTCTCCGCC CTAACACCAT CTACCTGTTC 
ATGGTGCGAG CGGTCAACGT CCAAGGCCTG AGCGACCCAA GCCCCATGTC TGAACCCGTC 
CGCACTCAAG ATATAAGTCC TCCAGCGCAG GGTGTGGATC ACAGACATGT GCAAAAGGAG 
CTGGGAGAAG TTATTGTGCG GCTACACAAC CCTGTGGTCC TTAGCCCTAC CACCATACAG 
GTCACATGGA CGGTTGACCG CCAGTCTCAG TTCATCCAGG GCTACAGGGT TTTGTACAGG 
CAGATGTCAG GCCTGTCTTC TCCAGGAGCC TGGCAAACTC AGGATGTGAA AGTCCCCTCA 
GAGAGGAGCA TGGTGCTCTC GGCTCTAAAG AAAGGCATCG TCTACGAGAT CAAAGTGCGT 
CCATATTTCA ATGAGTTTCA GGGCATGGAC AGCGAATCCC GAACCGCAAG AACAACAGAG 
GAAGCGCCCA GTGCTCCTCC TCAGCAGGTG ACTGTACTCA CTGTGGGCAA CCAGAACAGC 
ACATCTATTA GCATCTCCTG GGACCCTCCG CCTGCAGAAC ACCAGAACGG CATCATACAG 
GAGTACAAGA TCTGGTGTTT GGGGAATGAG ACCCGTTTTC ATGTGAATAA GACGGTGGAC 
GCAGCGATCC GCTCAGTGGT GGTTGGGGGT CTCCAGGCGG GGGTCCAGTA TCGGGTGGAG 
GTGGCCGCTA GCACCAGCGC CGGGGTCGGA GTGAAGAGCG AACCACAAAC CATCATCATT 
GGTAAAGACT TCCGCGATGT GATGATTAGC AGGAATAACA GCATCACAGA GCAGATCACG 
GATGTGGTGA AACAGCCGGC CTTCATCGCG GGCATTGGCG GGGCCTGCTG GGTCATTCTG 
ATGGGCTTCA GCATCTGGCT CTACTGGAGG AGGAAGAAGA GAAAGGGACT CAGCAATTAC 
GCAGTTCAGT CGTTCACCTT CACCCCTGCA GTGACGTTCC AGCGTGTTGA TGGAGGCTTG 
ATGAGCAACG GGAGTCGTCC TGGTCTGCTG AACGCCAGTG ATTCGGGTTA CCCGTGGCTC 
GCCGACTCCT GGCCTCCCAG CCTGCCTGTC AACAGCGGAT CTGGAGGACC CAATGACCTC 
AGCTTTGGCC GTGGAGATGT GATGCCCAGT GCTCAGGCTG ATAAGACGGG TACCATGCTT 
TCGGATGGAG CCATTTACAG CAGCATCGAC TTCACCACCA AAGGCGGATT CGGTAGCCCA 
AGCCCAGCCT CCCAGGCCAC GCCCTATGCC ACCACACAGA TCCTCCACTC CAACAGCATC 
CATGAGCTGG CAGTGGATCT TCCTGAAGCC CAGTGGAAGA CCTCCCTACA GGCCAAGCAG 
GAGATGGCCA GCCTGGGTTA CTCACTGCCC GACAAAAACT CCTGCAATAA CAGTGGAAAA 
GGTGGTAAGA AGAAGAAGAC CAAAGCAAAC GTCAAGCCCA CCAAGATGAA TGGCTCCAAC 
TGGGCGAACG TTCCACTTCC TCCTCCTCCC ATCCACCCTC TTCCTGGCAC AGAGATGGAC 
CACTACGCCT CAGAGCACCA TGATGGAGGA GGATATGATA GTGATGGTTG GGGCCCACCA 
TTGCCAGTGC AGACGTACCT CCATCAGGGT TTAGAGGATG AGCTGGAGGA AGAGGAAGAG 
CGGGTGCCAA CTCCTCCCAT TAGGGGTGTG GCTTCTTCTC CTGCTGCAGT TTCCTTTGGC 
AAACAGTCCA CAGCCACACT GAGCCCATCT CCACGTGAAG AAATGCAGCC CATGCTCCAA 
GCCCACCTAG ATGAGCTTAC CCGTGCTTAT CAATTGGACA TGGCTAAACA GACTTGGCAC 
ATGCAGGGTG GGCCTTACCC GCCCCAGGCC CCCGCACCAC CCGTGGGCTA CGTCTCCAGC 
ACCTTAGTGT CTGACATCGA GACCGATCTC CCTGATGAAG ATGACGATGA TGAGGAGGAT 
GAAGGATACG AGATGTCGCC ACCAATTAGG GGCACTGAAC ACACGCCAGG CTCCAGCATG 
GACAACTTAG ACAGCTCCGT TACGGGCTCT ATGGTGAACG GCTGGGGCTC GGCTTCAGAA 
GATGATGACA GAAACTTCTC CAGCAGGAGG TCTAGTGCCG GCAGCTCTTC TGACGGCTCG 
CTCTTCACCC ATTGCAGCTT TGCGCGAGCC CTGGTGGCAG CCGCAGACAA GGCTGGTTAC 
CGTCTGGATG GCACCAGCCT GAGCAAAAGA GATAAAGGTC TACCTCATAG ACCTCGTCCC 
GCTAGCCCGT TCTCCACAGA TGGAAGCACG GTGGGCACAC ACAGTCTGGG GCACCAGCGG 
GCAGCAAGGC CCACACGTAA ACACAAAGGC CCTGGCACTG TCCCGAACCG CCGTGATGCC 
AGTGCGGATG ATCTGCCTCC ACCTCCAGAG CCTCCACCCT GCCAGACAGC TCGCATCCCC 
GGGTCGAGGA ACGTTCCCCA TCCCTGCGAC CGCAAAGCTT CCTCTCTGGA GAGGCAGCCT 
ATGATGGGCC TTGAGGAAAT GAGAAGTTCC TTGGACCGGC AGGCCCGAAC CTCATTGGAC 
CGCCACCAAC AAGTCCAGGA CAGACTGGGG TCCATAGAGA GAGGAGATGA AAGCAGAAGA 
GGACAGAAAA ATGGTCCCTT TTCAGAAGAG GCCCTATTGC CATACAACAA ACCATCATTC 
CCATCCCCTG GTGGGCACAG CTCATCTGGG ACGGCCTCTT CTAAAGGGTC AACGGGTCCT 
CGCAAAGGAG AAGGGACGAG GGGACAGCAC CAGCGCTCCA CCACGGAACA AGGAGAAAGC 
AGCTACATGG GGACCAACGC GCAGTTTTCC GGAGAGTTAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

robo2 ( XM_021481458.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021337133.1 Danio rerio roundabout, axon guidance receptor, homolog 2 (Drosophila) (robo2), transcript variant X8, mRNA.

Price & Availability↑

CloneID	ODa148981
Clone ID Related Accession (Same CDS sequence)	XM_021481458.1
Accession Version	XM_021481458.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4635bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	roundabout homolog 2 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGTCT CCCCTGAGCG AGCGAGGTGT GCTGTGGTCC TGGCTGGACC TCCTCTCCTC 
CTCATTATCT GTGTCCTGTG GATCCTGTGC TCAGACTCAG TGGATGCTCA AGGGCAAGGA 
TCCCGTCTGC GGCAGGAAGA TTCTCCACCT CGGATTGTAG AACACCCCTC CGATCTGATC 
GTGTCAAAGG GCGAGCCAGC CACCCTAAAC TGCAAGGCGG AGGGTCGGCC AACTCCCACC 
GTGGAGTGGT ACAAGGATGG AGAACGAGTT GAAACGGACA AAGATGACCC TCGATCTCAC 
CGCATGCTCC TTCCCAGTGG TTCGCTCTTC TTCCTGCGTA TCGTACATGG ACGCCGCAGC 
AAACCTGATG AGGGGGCGTA CGTCTGTGTG GCACGCAACT ACTTGGGGGA AGCAGTCAGT 
CGAAACGCCT CGCTGGAAGT AGCATTGTTA CGGGATGATT TCCGGCAGAA CCCTACAGAT 
GTGGTGGTCG CTGCTGGAGA GCCGGCCATC CTGGAGTGTG TCCCTCCCAG AGGACACCCG 
GAGCCCACCA TCTACTGGAA GAAAGACAAA GTCCGAATTG ATGAGAAGGA TGACAGGATC 
AAGATCCGTG GAGGGAAGCT GATGATCTCT AACACCAGGA AAAGCGACGC TGGAATGTAC 
ATCTGCGTGG GCACCAACAT GGTCGGCGAA AGAGACAGTG AAACAGCCCA GGTCACTGTG 
TTCGAGAGGC CCACGTTCCT GCGGCGTCCC ATTAACCAGG TGGTTCTGGA GGAGGAGGCT 
GTGGAATTTC GATGTCAGGT GCAGGGAGAC CCGCAGCCGT CCATCCGCTG GAAAAAAGAC 
GACGTTGAAA TCCCCCGCGG CAGGTACGAT ATGAAATTTG ATAAAGATGA CTTCACGCTG 
AGAATAAAGA AGGCAACAGC GAGTGATGAA GGAACCTTCA CCTGTGTAGC GGAGAACCGA 
GTGGGCAAAA TGGAGGCCTC CGCAACCCTC ACGGTCAGAG CTCGCCCTGT CGCTGCACCG 
CAGTTCGTGA TCAGGCCGCG GGATCAGATT GTGGCACAGG GACGCACCGC CAGCTTCCCC 
TGCGAGACTA AAGGGAACCC GCAGCCGGCA GTCTTCTGGC AGAAAGAAGG AAGTCAGAAT 
CTCCTGTTTC CAAATCAACC CCAGCAGCCC AACAGTCGTT TCTCGGTGTC GCCCAGTGGA 
GATCTAACCA TCACTACGGT CCAGCGGGCA GATGCGGGCT ATTATATCTG CCAGGCCCTG 
ACGGTGGCGG GCAGCATCCT GGCCAAAGCA CAGCTTGAGG TCACAGACGT GTTGACCGAT 
CGCCCTCCAC CCATCATCCG GCAGGGTCCA GCTAACCAGA CTCTTGGTGT AGACAGTGTG 
GCTCTTTTGA GGTGCCATGC ATCCGGAGAA CCAGTCCCAA CCATCAGCTG GCTGAAGGAC 
GGGGTCAGTC TGCTGGGCAA AGACCCTCGG ATGTCCCTGC AAGACCTCGG CAGCCTGCAG 
ATCAAGGGTT TACGGCTCTC TGATTCTGGC ATTTATACTT GTGTGGCTGC AAGTTCTAGT 
GGGGAAACAT CATGGAGTGC TTTCTTGGAG GTCAAAGAGG CTGGGAGTCT TGTTGTAGTT 
AAAGATCGGG ATGAGAACGA GCTTCCAGGA CCTCCTTCCA AACCTCAGGT CACAGATGTC 
ACTAAAAACA GCGTGTCATT ATCCTGGCAG CCAGGCCTTC CCGGAGCATC AGCCATTTCT 
ACATATGTCA TAGAAGCATT CAGCCAATCG GTGAGCAACA GCTGGCAAAC TGTTGCCGAT 
CACGCCAAGA CAACGCAGTA CACCATTAAG GGTCTCCGCC CTAACACCAT CTACCTGTTC 
ATGGTGCGAG CGGTCAACGT CCAAGGCCTG AGCGACCCAA GCCCCATGTC TGAACCCGTC 
CGCACTCAAG ATATAAGTCC TCCAGCGCAG GGTGTGGATC ACAGACATGT GCAAAAGGAG 
CTGGGAGAAG TTATTGTGCG GCTACACAAC CCTGTGGTCC TTAGCCCTAC CACCATACAG 
GTCACATGGA CGGTTGACCG CCAGTCTCAG TTCATCCAGG GCTACAGGGT TTTGTACAGG 
CAGATGTCAG GCCTGTCTTC TCCAGGAGCC TGGCAAACTC AGGATGTGAA AGTCCCCTCA 
GAGAGGAGCA TGGTGCTCTC GGCTCTAAAG AAAGGCATCG TCTACGAGAT CAAAGTGCGT 
CCATATTTCA ATGAGTTTCA GGGCATGGAC AGCGAATCCC GAACCGCAAG AACAACAGAG 
GAAGCGCCCA GTGCTCCTCC TCAGCAGGTG ACTGTACTCA CTGTGGGCAA CCAGAACAGC 
ACATCTATTA GCATCTCCTG GGACCCTCCG CCTGCAGAAC ACCAGAACGG CATCATACAG 
GAGTACAAGA TCTGGTGTTT GGGGAATGAG ACCCGTTTTC ATGTGAATAA GACGGTGGAC 
GCAGCGATCC GCTCAGTGGT GGTTGGGGGT CTCCAGGCGG GGGTCCAGTA TCGGGTGGAG 
GTGGCCGCTA GCACCAGCGC CGGGGTCGGA GTGAAGAGCG AACCACAAAC CATCATCATT 
GGTAAAGACT TCCGCGATGT GATGATTAGC AGGAATAACA GCATCACAGA GCAGATCACG 
GATGTGGTGA AACAGCCGGC CTTCATCGCG GGCATTGGCG GGGCCTGCTG GGTCATTCTG 
ATGGGCTTCA GCATCTGGCT CTACTGGAGG AGGAAGAAGA GAAAGGGACT CAGCAATTAC 
GCAGTGACGT TCCAGCGTGT TGATGGAGGC TTGATGAGCA ACGGGAGTCG TCCTGGTCTG 
CTGAACGCCA GTGATTCGGG TTACCCGTGG CTCGCCGACT CCTGGCCTCC CAGCCTGCCT 
GTCAACAGCG GATCTGGAGG ACCCAATGAC CTCAGCTTTG GCCGTGGAGA TGTGATGCCC 
AGTGCTCAGG CTGATAAGAC GGGTACCATG CTTTCGGATG GAGCCATTTA CAGCAGCATC 
GACTTCACCA CCAAAGGCGG ATTCGGTAGC CCAAGCCCAG CCTCCCAGGC CACGCCCTAT 
GCCACCACAC AGATCCTCCA CTCCAACAGC ATCCATGAGC TGGCAGTGGA TCTTCCTGAA 
GCCCAGTGGA AGACCTCCCT ACAGGCCAAG CAGGAGATGG CCAGCCTGGG TTACTCACTG 
CCCGACAAAA ACTCCTGCAA TAACAGTGGA AAAGGTGGTA AGAAGAAGAA GACCAAAGCA 
AACGTCAAGC CCACCAAGAT GAATGGCTCC AACTGGGCGA ACGTTCCACT TCCTCCTCCT 
CCCATCCACC CTCTTCCTGG CACAGAGATG GACCACTACG CCTCAGAGCA CCATGATGGA 
GGAGGATATG ATAGTGATGG TTGGGGCCCA CCATTGCCAG TGCAGACGTA CCTCCATCAG 
GGTTTAGAGG ATGAGCTGGA GGAAGAGGAA GAGCGGGTGC CAACTCCTCC CATTAGGGGT 
GTGGCTTCTT CTCCTGCTGC AGTTTCCTTT GGCAAACAGT CCACAGCCAC ACTGAGCCCA 
TCTCCACGTG AAGAAATGCA GCCCATGCTC CAAGCCCACC TAGATGAGCT TACCCGTGCT 
TATCAATTGG ACATGGCTAA ACAGACTTGG CACATGCAGG GTGGGCCTTA CCCGCCCCAG 
GCCCCCGCAC CACCCGTGGG CTACGTCTCC AGCACCTTAG TGTCTGACAT CGAGACCGAT 
CTCCCTGATG AAGATGACGA TGATGAGGAG GATGAAGGAT ACGAGATGTC GCCACCAATT 
AGGGGCACTG AACACACGCC AGGCTCCAGC ATGGACAACT TAGACAGCTC CGTTACGGGC 
TCTATGGTGA ACGGCTGGGG CTCGGCTTCA GAAGATGATG ACAGAAACTT CTCCAGCAGG 
AGGTCTAGTG CCGGCAGCTC TTCTGACGGC TCGCTCTTCA CCCATTGCAG CTTTGCGCGA 
GCCCTGGTGG CAGCCGCAGA CAAGGCTGGT TACCGTCTGG ATGGCACCAG CCTGAGCAAA 
AGAGATAAAG GTCTACCTCA TAGACCTCGT CCCGCTAGCC CGTTCTCCAC AGATGGAAGC 
ACGGTGGGCA CACACAGTCT GGGGCACCAG CGGGCAGCAA GGCCCACACG TAAACACAAA 
GGCCCTGGCA CTGTCCCGAA CCGCCGTGAT GCCAGTGCGG ATGATCTGCC TCCACCTCCA 
GAGCCTCCAC CCTGCCAGAC AGCTCGCATC CCCGGGTCGA GGAACGTTCC CCATCCCTGC 
GACCGCAAAG CTTCCTCTCT GGAGAGGCAG CCTATGATGG GCCTTGAGGA AATGAGAAGT 
TCCTTGGACC GGCAGGCCCG AACCTCATTG GACCGCCACC AACAAGTCCA GGACAGACTG 
GGGTCCATAG AGAGAGGAGA TGAAAGCAGA AGAGGACAGA AAAATGGTCC CTTTTCAGAA 
GAGGCCCTAT TGCCATACAA CAAACCATCA TTCCCATCCC CTGGTGGGCA CAGCTCATCT 
GGGACGGCCT CTTCTAAAGG GTCAACGGGT CCTCGCAAAG GAGAAGGGAC GAGGGGACAG 
CACCAGCGCT CCACCACGGA ACAAGGAGAA AGCAGCTACA TGGGGACCAA CGCGCAGTTT 
TCCGGAGAGT TATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021337133.1
CDS	252..4886
Translation	MAVSPERARCAVVLAGPPLLLIICVLWILCSDSVDAQGQGSRLRQEDSPPRIVEHPSDLIVSKGEPATLNCKAEGRPTPTVEWYKDGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRRSKPDEGAYVCVARNYLGEAVSRNASLEVALLRDDFRQNPTDVVVAAGEPAILECVPPRGHPEPTIYWKKDKVRIDEKDDRIKIRGGKLMISNTRKSDAGMYICVGTNMVGERDSETAQVTVFERPTFLRRPINQVVLEEEAVEFRCQVQGDPQPSIRWKKDDVEIPRGRYDMKFDKDDFTLRIKKATASDEGTFTCVAENRVGKMEASATLTVRARPVAAPQFVIRPRDQIVAQGRTASFPCETKGNPQPAVFWQKEGSQNLLFPNQPQQPNSRFSVSPSGDLTITTVQRADAGYYICQALTVAGSILAKAQLEVTDVLTDRPPPIIRQGPANQTLGVDSVALLRCHASGEPVPTISWLKDGVSLLGKDPRMSLQDLGSLQIKGLRLSDSGIYTCVAASSSGETSWSAFLEVKEAGSLVVVKDRDENELPGPPSKPQVTDVTKNSVSLSWQPGLPGASAISTYVIEAFSQSVSNSWQTVADHAKTTQYTIKGLRPNTIYLFMVRAVNVQGLSDPSPMSEPVRTQDISPPAQGVDHRHVQKELGEVIVRLHNPVVLSPTTIQVTWTVDRQSQFIQGYRVLYRQMSGLSSPGAWQTQDVKVPSERSMVLSALKKGIVYEIKVRPYFNEFQGMDSESRTARTTEEAPSAPPQQVTVLTVGNQNSTSISISWDPPPAEHQNGIIQEYKIWCLGNETRFHVNKTVDAAIRSVVVGGLQAGVQYRVEVAASTSAGVGVKSEPQTIIIGKDFRDVMISRNNSITEQITDVVKQPAFIAGIGGACWVILMGFSIWLYWRRKKRKGLSNYAVTFQRVDGGLMSNGSRPGLLNASDSGYPWLADSWPPSLPVNSGSGGPNDLSFGRGDVMPSAQADKTGTMLSDGAIYSSIDFTTKGGFGSPSPASQATPYATTQILHSNSIHELAVDLPEAQWKTSLQAKQEMASLGYSLPDKNSCNNSGKGGKKKKTKANVKPTKMNGSNWANVPLPPPPIHPLPGTEMDHYASEHHDGGGYDSDGWGPPLPVQTYLHQGLEDELEEEEERVPTPPIRGVASSPAAVSFGKQSTATLSPSPREEMQPMLQAHLDELTRAYQLDMAKQTWHMQGGPYPPQAPAPPVGYVSSTLVSDIETDLPDEDDDDEEDEGYEMSPPIRGTEHTPGSSMDNLDSSVTGSMVNGWGSASEDDDRNFSSRRSSAGSSSDGSLFTHCSFARALVAAADKAGYRLDGTSLSKRDKGLPHRPRPASPFSTDGSTVGTHSLGHQRAARPTRKHKGPGTVPNRRDASADDLPPPPEPPPCQTARIPGSRNVPHPCDRKASSLERQPMMGLEEMRSSLDRQARTSLDRHQQVQDRLGSIERGDESRRGQKNGPFSEEALLPYNKPSFPSPGGHSSSGTASSKGSTGPRKGEGTRGQHQRSTTEQGESSYMGTNAQFSGEL

Target ORF information:

RefSeq Version	XM_021481458.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio roundabout, axon guidance receptor, homolog 2 (Drosophila) (robo2), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021481458.1

ORF Insert Sequence:

ATGGCTGTCT CCCCTGAGCG AGCGAGGTGT GCTGTGGTCC TGGCTGGACC TCCTCTCCTC 
CTCATTATCT GTGTCCTGTG GATCCTGTGC TCAGACTCAG TGGATGCTCA AGGGCAAGGA 
TCCCGTCTGC GGCAGGAAGA TTCTCCACCT CGGATTGTAG AACACCCCTC CGATCTGATC 
GTGTCAAAGG GCGAGCCAGC CACCCTAAAC TGCAAGGCGG AGGGTCGGCC AACTCCCACC 
GTGGAGTGGT ACAAGGATGG AGAACGAGTT GAAACGGACA AAGATGACCC TCGATCTCAC 
CGCATGCTCC TTCCCAGTGG TTCGCTCTTC TTCCTGCGTA TCGTACATGG ACGCCGCAGC 
AAACCTGATG AGGGGGCGTA CGTCTGTGTG GCACGCAACT ACTTGGGGGA AGCAGTCAGT 
CGAAACGCCT CGCTGGAAGT AGCATTGTTA CGGGATGATT TCCGGCAGAA CCCTACAGAT 
GTGGTGGTCG CTGCTGGAGA GCCGGCCATC CTGGAGTGTG TCCCTCCCAG AGGACACCCG 
GAGCCCACCA TCTACTGGAA GAAAGACAAA GTCCGAATTG ATGAGAAGGA TGACAGGATC 
AAGATCCGTG GAGGGAAGCT GATGATCTCT AACACCAGGA AAAGCGACGC TGGAATGTAC 
ATCTGCGTGG GCACCAACAT GGTCGGCGAA AGAGACAGTG AAACAGCCCA GGTCACTGTG 
TTCGAGAGGC CCACGTTCCT GCGGCGTCCC ATTAACCAGG TGGTTCTGGA GGAGGAGGCT 
GTGGAATTTC GATGTCAGGT GCAGGGAGAC CCGCAGCCGT CCATCCGCTG GAAAAAAGAC 
GACGTTGAAA TCCCCCGCGG CAGGTACGAT ATGAAATTTG ATAAAGATGA CTTCACGCTG 
AGAATAAAGA AGGCAACAGC GAGTGATGAA GGAACCTTCA CCTGTGTAGC GGAGAACCGA 
GTGGGCAAAA TGGAGGCCTC CGCAACCCTC ACGGTCAGAG CTCGCCCTGT CGCTGCACCG 
CAGTTCGTGA TCAGGCCGCG GGATCAGATT GTGGCACAGG GACGCACCGC CAGCTTCCCC 
TGCGAGACTA AAGGGAACCC GCAGCCGGCA GTCTTCTGGC AGAAAGAAGG AAGTCAGAAT 
CTCCTGTTTC CAAATCAACC CCAGCAGCCC AACAGTCGTT TCTCGGTGTC GCCCAGTGGA 
GATCTAACCA TCACTACGGT CCAGCGGGCA GATGCGGGCT ATTATATCTG CCAGGCCCTG 
ACGGTGGCGG GCAGCATCCT GGCCAAAGCA CAGCTTGAGG TCACAGACGT GTTGACCGAT 
CGCCCTCCAC CCATCATCCG GCAGGGTCCA GCTAACCAGA CTCTTGGTGT AGACAGTGTG 
GCTCTTTTGA GGTGCCATGC ATCCGGAGAA CCAGTCCCAA CCATCAGCTG GCTGAAGGAC 
GGGGTCAGTC TGCTGGGCAA AGACCCTCGG ATGTCCCTGC AAGACCTCGG CAGCCTGCAG 
ATCAAGGGTT TACGGCTCTC TGATTCTGGC ATTTATACTT GTGTGGCTGC AAGTTCTAGT 
GGGGAAACAT CATGGAGTGC TTTCTTGGAG GTCAAAGAGG CTGGGAGTCT TGTTGTAGTT 
AAAGATCGGG ATGAGAACGA GCTTCCAGGA CCTCCTTCCA AACCTCAGGT CACAGATGTC 
ACTAAAAACA GCGTGTCATT ATCCTGGCAG CCAGGCCTTC CCGGAGCATC AGCCATTTCT 
ACATATGTCA TAGAAGCATT CAGCCAATCG GTGAGCAACA GCTGGCAAAC TGTTGCCGAT 
CACGCCAAGA CAACGCAGTA CACCATTAAG GGTCTCCGCC CTAACACCAT CTACCTGTTC 
ATGGTGCGAG CGGTCAACGT CCAAGGCCTG AGCGACCCAA GCCCCATGTC TGAACCCGTC 
CGCACTCAAG ATATAAGTCC TCCAGCGCAG GGTGTGGATC ACAGACATGT GCAAAAGGAG 
CTGGGAGAAG TTATTGTGCG GCTACACAAC CCTGTGGTCC TTAGCCCTAC CACCATACAG 
GTCACATGGA CGGTTGACCG CCAGTCTCAG TTCATCCAGG GCTACAGGGT TTTGTACAGG 
CAGATGTCAG GCCTGTCTTC TCCAGGAGCC TGGCAAACTC AGGATGTGAA AGTCCCCTCA 
GAGAGGAGCA TGGTGCTCTC GGCTCTAAAG AAAGGCATCG TCTACGAGAT CAAAGTGCGT 
CCATATTTCA ATGAGTTTCA GGGCATGGAC AGCGAATCCC GAACCGCAAG AACAACAGAG 
GAAGCGCCCA GTGCTCCTCC TCAGCAGGTG ACTGTACTCA CTGTGGGCAA CCAGAACAGC 
ACATCTATTA GCATCTCCTG GGACCCTCCG CCTGCAGAAC ACCAGAACGG CATCATACAG 
GAGTACAAGA TCTGGTGTTT GGGGAATGAG ACCCGTTTTC ATGTGAATAA GACGGTGGAC 
GCAGCGATCC GCTCAGTGGT GGTTGGGGGT CTCCAGGCGG GGGTCCAGTA TCGGGTGGAG 
GTGGCCGCTA GCACCAGCGC CGGGGTCGGA GTGAAGAGCG AACCACAAAC CATCATCATT 
GGTAAAGACT TCCGCGATGT GATGATTAGC AGGAATAACA GCATCACAGA GCAGATCACG 
GATGTGGTGA AACAGCCGGC CTTCATCGCG GGCATTGGCG GGGCCTGCTG GGTCATTCTG 
ATGGGCTTCA GCATCTGGCT CTACTGGAGG AGGAAGAAGA GAAAGGGACT CAGCAATTAC 
GCAGTGACGT TCCAGCGTGT TGATGGAGGC TTGATGAGCA ACGGGAGTCG TCCTGGTCTG 
CTGAACGCCA GTGATTCGGG TTACCCGTGG CTCGCCGACT CCTGGCCTCC CAGCCTGCCT 
GTCAACAGCG GATCTGGAGG ACCCAATGAC CTCAGCTTTG GCCGTGGAGA TGTGATGCCC 
AGTGCTCAGG CTGATAAGAC GGGTACCATG CTTTCGGATG GAGCCATTTA CAGCAGCATC 
GACTTCACCA CCAAAGGCGG ATTCGGTAGC CCAAGCCCAG CCTCCCAGGC CACGCCCTAT 
GCCACCACAC AGATCCTCCA CTCCAACAGC ATCCATGAGC TGGCAGTGGA TCTTCCTGAA 
GCCCAGTGGA AGACCTCCCT ACAGGCCAAG CAGGAGATGG CCAGCCTGGG TTACTCACTG 
CCCGACAAAA ACTCCTGCAA TAACAGTGGA AAAGGTGGTA AGAAGAAGAA GACCAAAGCA 
AACGTCAAGC CCACCAAGAT GAATGGCTCC AACTGGGCGA ACGTTCCACT TCCTCCTCCT 
CCCATCCACC CTCTTCCTGG CACAGAGATG GACCACTACG CCTCAGAGCA CCATGATGGA 
GGAGGATATG ATAGTGATGG TTGGGGCCCA CCATTGCCAG TGCAGACGTA CCTCCATCAG 
GGTTTAGAGG ATGAGCTGGA GGAAGAGGAA GAGCGGGTGC CAACTCCTCC CATTAGGGGT 
GTGGCTTCTT CTCCTGCTGC AGTTTCCTTT GGCAAACAGT CCACAGCCAC ACTGAGCCCA 
TCTCCACGTG AAGAAATGCA GCCCATGCTC CAAGCCCACC TAGATGAGCT TACCCGTGCT 
TATCAATTGG ACATGGCTAA ACAGACTTGG CACATGCAGG GTGGGCCTTA CCCGCCCCAG 
GCCCCCGCAC CACCCGTGGG CTACGTCTCC AGCACCTTAG TGTCTGACAT CGAGACCGAT 
CTCCCTGATG AAGATGACGA TGATGAGGAG GATGAAGGAT ACGAGATGTC GCCACCAATT 
AGGGGCACTG AACACACGCC AGGCTCCAGC ATGGACAACT TAGACAGCTC CGTTACGGGC 
TCTATGGTGA ACGGCTGGGG CTCGGCTTCA GAAGATGATG ACAGAAACTT CTCCAGCAGG 
AGGTCTAGTG CCGGCAGCTC TTCTGACGGC TCGCTCTTCA CCCATTGCAG CTTTGCGCGA 
GCCCTGGTGG CAGCCGCAGA CAAGGCTGGT TACCGTCTGG ATGGCACCAG CCTGAGCAAA 
AGAGATAAAG GTCTACCTCA TAGACCTCGT CCCGCTAGCC CGTTCTCCAC AGATGGAAGC 
ACGGTGGGCA CACACAGTCT GGGGCACCAG CGGGCAGCAA GGCCCACACG TAAACACAAA 
GGCCCTGGCA CTGTCCCGAA CCGCCGTGAT GCCAGTGCGG ATGATCTGCC TCCACCTCCA 
GAGCCTCCAC CCTGCCAGAC AGCTCGCATC CCCGGGTCGA GGAACGTTCC CCATCCCTGC 
GACCGCAAAG CTTCCTCTCT GGAGAGGCAG CCTATGATGG GCCTTGAGGA AATGAGAAGT 
TCCTTGGACC GGCAGGCCCG AACCTCATTG GACCGCCACC AACAAGTCCA GGACAGACTG 
GGGTCCATAG AGAGAGGAGA TGAAAGCAGA AGAGGACAGA AAAATGGTCC CTTTTCAGAA 
GAGGCCCTAT TGCCATACAA CAAACCATCA TTCCCATCCC CTGGTGGGCA CAGCTCATCT 
GGGACGGCCT CTTCTAAAGG GTCAACGGGT CCTCGCAAAG GAGAAGGGAC GAGGGGACAG 
CACCAGCGCT CCACCACGGA ACAAGGAGAA AGCAGCTACA TGGGGACCAA CGCGCAGTTT 
TCCGGAGAGT TATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

robo2 ( XM_021481459.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021337134.1 Danio rerio roundabout, axon guidance receptor, homolog 2 (Drosophila) (robo2), transcript variant X9, mRNA.

Price & Availability↑

CloneID	ODa148982
Clone ID Related Accession (Same CDS sequence)	XM_021481459.1
Accession Version	XM_021481459.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4602bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	roundabout homolog 2 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGTCT CCCCTGAGCG AGCGAGGTGT GCTGTGGTCC TGGCTGGACC TCCTCTCCTC 
CTCATTATCT GTGTCCTGTG GATCCTGTGC TCAGACTCAG TGGATGCTCA AGGGCAAGGA 
TCCCGTCTGC GGCAGGAAGA TTCTCCACCT CGGATTGTAG AACACCCCTC CGATCTGATC 
GTGTCAAAGG GCGAGCCAGC CACCCTAAAC TGCAAGGCGG AGGGTCGGCC AACTCCCACC 
GTGGAGTGGT ACAAGGATGG AGAACGAGTT GAAACGGACA AAGATGACCC TCGATCTCAC 
CGCATGCTCC TTCCCAGTGG TTCGCTCTTC TTCCTGCGTA TCGTACATGG ACGCCGCAGC 
AAACCTGATG AGGGGGCGTA CGTCTGTGTG GCACGCAACT ACTTGGGGGA AGCAGTCAGT 
CGAAACGCCT CGCTGGAAGT AGCATTGTTA CGGGATGATT TCCGGCAGAA CCCTACAGAT 
GTGGTGGTCG CTGCTGGAGA GCCGGCCATC CTGGAGTGTG TCCCTCCCAG AGGACACCCG 
GAGCCCACCA TCTACTGGAA GAAAGACAAA GTCCGAATTG ATGAGAAGGA TGACAGGATC 
AAGATCCGTG GAGGGAAGCT GATGATCTCT AACACCAGGA AAAGCGACGC TGGAATGTAC 
ATCTGCGTGG GCACCAACAT GGTCGGCGAA AGAGACAGTG AAACAGCCCA GGTCACTGTG 
TTCGAGAGGC CCACGTTCCT GCGGCGTCCC ATTAACCAGG TGGTTCTGGA GGAGGAGGCT 
GTGGAATTTC GATGTCAGGT GCAGGGAGAC CCGCAGCCGT CCATCCGCTG GAAAAAAGAC 
GACGTTGAAA TCCCCCGCGG CAGGTACGAT ATGAAATTTG ATAAAGATGA CTTCACGCTG 
AGAATAAAGA AGGCAACAGC GAGTGATGAA GGAACCTTCA CCTGTGTAGC GGAGAACCGA 
GTGGGCAAAA TGGAGGCCTC CGCAACCCTC ACGGTCAGAG CTCGCCCTGT CGCTGCACCG 
CAGTTCGTGA TCAGGCCGCG GGATCAGATT GTGGCACAGG GACGCACCGC CAGCTTCCCC 
TGCGAGACTA AAGGGAACCC GCAGCCGGCA GTCTTCTGGC AGAAAGAAGG AAGTCAGAAT 
CTCCTGTTTC CAAATCAACC CCAGCAGCCC AACAGTCGTT TCTCGGTGTC GCCCAGTGGA 
GATCTAACCA TCACTACGGT CCAGCGGGCA GATGCGGGCT ATTATATCTG CCAGGCCCTG 
ACGGTGGCGG GCAGCATCCT GGCCAAAGCA CAGCTTGAGG TCACAGACGT GTTGACCGAT 
CGCCCTCCAC CCATCATCCG GCAGGGTCCA GCTAACCAGA CTCTTGGTGT AGACAGTGTG 
GCTCTTTTGA GGTGCCATGC ATCCGGAGAA CCAGTCCCAA CCATCAGCTG GCTGAAGGAC 
GGGGTCAGTC TGCTGGGCAA AGACCCTCGG ATGTCCCTGC AAGACCTCGG CAGCCTGCAG 
ATCAAGGGTT TACGGCTCTC TGATTCTGGC ATTTATACTT GTGTGGCTGC AAGTTCTAGT 
GGGGAAACAT CATGGAGTGC TTTCTTGGAG GTCAAAGAGG CTGGGAGTCT TGTTGTAGTT 
AAAGATCGGG ATGAGAACGA GCTTCCAGGA CCTCCTTCCA AACCTCAGGT CACAGATGTC 
ACTAAAAACA GCGTGTCATT ATCCTGGCAG CCAGGCCTTC CCGGAGCATC AGCCATTTCT 
ACATATGTCA TAGAAGCATT CAGCCAATCG GTGAGCAACA GCTGGCAAAC TGTTGCCGAT 
CACGCCAAGA CAACGCAGTA CACCATTAAG GGTCTCCGCC CTAACACCAT CTACCTGTTC 
ATGGTGCGAG CGGTCAACGT CCAAGGCCTG AGCGACCCAA GCCCCATGTC TGAACCCGTC 
CGCACTCAAG ATATAAGTCC TCCAGCGCAG GGTGTGGATC ACAGACATGT GCAAAAGGAG 
CTGGGAGAAG TTATTGTGCG GCTACACAAC CCTGTGGTCC TTAGCCCTAC CACCATACAG 
GTCACATGGA CGGTTGACCG CCAGTCTCAG TTCATCCAGG GCTACAGGGT TTTGTACAGG 
CAGATGTCAG GCCTGTCTTC TCCAGGAGCC TGGCAAACTC AGGATGTGAA AGTCCCCTCA 
GAGAGGAGCA TGGTGCTCTC GGCTCTAAAG AAAGGCATCG TCTACGAGAT CAAAGTGCGT 
CCATATTTCA ATGAGTTTCA GGGCATGGAC AGCGAATCCC GAACCGCAAG AACAACAGAG 
GAAGCGCCCA GTGCTCCTCC TCAGCAGGTG ACTGTACTCA CTGTGGGCAA CCAGAACAGC 
ACATCTATTA GCATCTCCTG GGACCCTCCG CCTGCAGAAC ACCAGAACGG CATCATACAG 
GAGTACAAGA TCTGGTGTTT GGGGAATGAG ACCCGTTTTC ATGTGAATAA GACGGTGGAC 
GCAGCGATCC GCTCAGTGGT GGTTGGGGGT CTCCAGGCGG GGGTCCAGTA TCGGGTGGAG 
GTGGCCGCTA GCACCAGCGC CGGGGTCGGA GTGAAGAGCG AACCACAAAC CATCATCATT 
GGTAAAGACT TCCGCGATGT GATGATTAGC AGGAATAACA GCATCACAGA GCAGATCACG 
GATGTGGTGA AACAGCCGGC CTTCATCGCG GGCATTGGCG GGGCCTGCTG GGTCATTCTG 
ATGGGCTTCA GCATCTGGCT CTACTGGAGG AGGAAGAAGA GAAAGGGACT CAGCAATTAC 
GCAGTTCAGT CGTTCACCTT CACCCCTGCA GTGACGTTCC AGCGTGTTGA TGGAGGCTTG 
ATGAGCAACG GGAGTCGTCC TGGTCTGCTG AACGCCAGTG ATTCGGGTTA CCCGTGGCTC 
GCCGACTCCT GGCCTCCCAG CCTGCCTGTC AACAGCGGAT CTGGAGGACC CAATGACCTC 
AGCTTTGGCC GTGGAGATGT GATGCCCAGT GCTCAGGCTG ATAAGACGGG TACCATGCTT 
TCGGATGGAG CCATTTACAG CAGCATCGAC TTCACCACCA AAGGCGGATT CGGTAGCCCA 
AGCCCAGCCT CCCAGGCCAC GCCCTATGCC ACCACACAGA TCCTCCACTC CAACAGCATC 
CATGAGCTGG CAGTGGATCT TCCTGAAGCC CAGTGGAAGA CCTCCCTACA GGCCAAGCAG 
GAGATGGCCA GCCTGGGTTA CTCACTGCCC GACAAAAACT CCTGCAATAA CACACTCCTT 
TTCATTCCTG ACTACCGATT GGCTGACGGA CTGTCTAATA GAATGCCACA CAACCAATCG 
CAGGATTTCA GCACCACCAG CTCCCACAAC AGCTCAGATC GGAGCAACAG TCTGTCAGGT 
GGAAAAGGTG GTAAGAAGAA GAAGACCAAA GCAAACGTCA AGCCCACCAA GATGAATGGC 
TCCAACTGGG CGAACGTTCC ACTTCCTCCT CCTCCCATCC ACCCTCTTCC TGGCACAGAG 
ATGGACCACT ACGCCTCAGA GCACCATGAT GGAGGAGGAT ATGATAGTGA TGGTTGGGGC 
CCACCATTGC CAGTGCAGAC GTACCTCCAT CAGGGTTTAG AGGATGAGCT GGAGGAAGAG 
GAAGAGCGGG TGCCAACTCC TCCCATTAGG GGTGTGGCTT CTTCTCCTGC TGCAGTTTCC 
TTTGGCAAAC AGTCCACAGC CACACTGAGC CCATCTCCAC GTGAAGAAAT GCAGCCCATG 
CTCCAAGCCC ACCTAGATGA GCTTACCCGT GCTTATCAAT TGGACATGGC TAAACAGACT 
TGGCACATGC AGGGTGGGCC TTACCCGCCC CAGGCCCCCG CACCACCCGT GGGCTACGTC 
TCCAGCACCT TAGTGTCTGA CATCGAGACC GATCTCCCTG ATGAAGATGA CGATGATGAG 
GAGGATGAAG GATACGAGAT GTCGCCACCA ATTAGGGGCA CTGAACACAC GCCAGGCTCC 
AGCATGGACA ACTTAGACAG CTCCGTTACG GATAAAGGTC TACCTCATAG ACCTCGTCCC 
GCTAGCCCGT TCTCCACAGA TGGAAGCACG GTGGGCACAC ACAGTCTGGG GCACCAGCGG 
GCAGCAAGGC CCACACGTAA ACACAAAGGC CCTGGCACTG TCCCGAACCG CCGTGATGCC 
AGTGCGGATG ATCTGCCTCC ACCTCCAGAG CCTCCACCCT GCCAGACAGC TCGCATCCCC 
GGGTCGAGGA ACGTTCCCCA TCCCTGCGAC CGCAAAGCTT CCTCTCTGGA GAGGCAGCCT 
ATGATGGGCC TTGAGGAAAT GAGAAGTTCC TTGGACCGGC AGGCCCGAAC CTCATTGGAC 
CGCCACCAAC AAGTCCAGGA CAGACTGGGG TCCATAGAGA GAGGAGATGA AAGCAGAAGA 
GGACAGAAAA ATGGTCCCTT TTCAGAAGAG GCCCTATTGC CATACAACAA ACCATCATTC 
CCATCCCCTG GTGGGCACAG CTCATCTGGG ACGGCCTCTT CTAAAGGGTC AACGGGTCCT 
CGCAAAGGAG AAGGGACGAG GGGACAGCAC CAGCGCTCCA CCACGGAACA AGGAGAAAGC 
AGCTACATGG GGACCAACGC GCAGTTTTCC GGAGAGTTAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021337134.1
CDS	252..4853
Translation	MAVSPERARCAVVLAGPPLLLIICVLWILCSDSVDAQGQGSRLRQEDSPPRIVEHPSDLIVSKGEPATLNCKAEGRPTPTVEWYKDGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRRSKPDEGAYVCVARNYLGEAVSRNASLEVALLRDDFRQNPTDVVVAAGEPAILECVPPRGHPEPTIYWKKDKVRIDEKDDRIKIRGGKLMISNTRKSDAGMYICVGTNMVGERDSETAQVTVFERPTFLRRPINQVVLEEEAVEFRCQVQGDPQPSIRWKKDDVEIPRGRYDMKFDKDDFTLRIKKATASDEGTFTCVAENRVGKMEASATLTVRARPVAAPQFVIRPRDQIVAQGRTASFPCETKGNPQPAVFWQKEGSQNLLFPNQPQQPNSRFSVSPSGDLTITTVQRADAGYYICQALTVAGSILAKAQLEVTDVLTDRPPPIIRQGPANQTLGVDSVALLRCHASGEPVPTISWLKDGVSLLGKDPRMSLQDLGSLQIKGLRLSDSGIYTCVAASSSGETSWSAFLEVKEAGSLVVVKDRDENELPGPPSKPQVTDVTKNSVSLSWQPGLPGASAISTYVIEAFSQSVSNSWQTVADHAKTTQYTIKGLRPNTIYLFMVRAVNVQGLSDPSPMSEPVRTQDISPPAQGVDHRHVQKELGEVIVRLHNPVVLSPTTIQVTWTVDRQSQFIQGYRVLYRQMSGLSSPGAWQTQDVKVPSERSMVLSALKKGIVYEIKVRPYFNEFQGMDSESRTARTTEEAPSAPPQQVTVLTVGNQNSTSISISWDPPPAEHQNGIIQEYKIWCLGNETRFHVNKTVDAAIRSVVVGGLQAGVQYRVEVAASTSAGVGVKSEPQTIIIGKDFRDVMISRNNSITEQITDVVKQPAFIAGIGGACWVILMGFSIWLYWRRKKRKGLSNYAVQSFTFTPAVTFQRVDGGLMSNGSRPGLLNASDSGYPWLADSWPPSLPVNSGSGGPNDLSFGRGDVMPSAQADKTGTMLSDGAIYSSIDFTTKGGFGSPSPASQATPYATTQILHSNSIHELAVDLPEAQWKTSLQAKQEMASLGYSLPDKNSCNNTLLFIPDYRLADGLSNRMPHNQSQDFSTTSSHNSSDRSNSLSGGKGGKKKKTKANVKPTKMNGSNWANVPLPPPPIHPLPGTEMDHYASEHHDGGGYDSDGWGPPLPVQTYLHQGLEDELEEEEERVPTPPIRGVASSPAAVSFGKQSTATLSPSPREEMQPMLQAHLDELTRAYQLDMAKQTWHMQGGPYPPQAPAPPVGYVSSTLVSDIETDLPDEDDDDEEDEGYEMSPPIRGTEHTPGSSMDNLDSSVTDKGLPHRPRPASPFSTDGSTVGTHSLGHQRAARPTRKHKGPGTVPNRRDASADDLPPPPEPPPCQTARIPGSRNVPHPCDRKASSLERQPMMGLEEMRSSLDRQARTSLDRHQQVQDRLGSIERGDESRRGQKNGPFSEEALLPYNKPSFPSPGGHSSSGTASSKGSTGPRKGEGTRGQHQRSTTEQGESSYMGTNAQFSGEL

Target ORF information:

RefSeq Version	XM_021481459.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio roundabout, axon guidance receptor, homolog 2 (Drosophila) (robo2), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021481459.1

ORF Insert Sequence:

ATGGCTGTCT CCCCTGAGCG AGCGAGGTGT GCTGTGGTCC TGGCTGGACC TCCTCTCCTC 
CTCATTATCT GTGTCCTGTG GATCCTGTGC TCAGACTCAG TGGATGCTCA AGGGCAAGGA 
TCCCGTCTGC GGCAGGAAGA TTCTCCACCT CGGATTGTAG AACACCCCTC CGATCTGATC 
GTGTCAAAGG GCGAGCCAGC CACCCTAAAC TGCAAGGCGG AGGGTCGGCC AACTCCCACC 
GTGGAGTGGT ACAAGGATGG AGAACGAGTT GAAACGGACA AAGATGACCC TCGATCTCAC 
CGCATGCTCC TTCCCAGTGG TTCGCTCTTC TTCCTGCGTA TCGTACATGG ACGCCGCAGC 
AAACCTGATG AGGGGGCGTA CGTCTGTGTG GCACGCAACT ACTTGGGGGA AGCAGTCAGT 
CGAAACGCCT CGCTGGAAGT AGCATTGTTA CGGGATGATT TCCGGCAGAA CCCTACAGAT 
GTGGTGGTCG CTGCTGGAGA GCCGGCCATC CTGGAGTGTG TCCCTCCCAG AGGACACCCG 
GAGCCCACCA TCTACTGGAA GAAAGACAAA GTCCGAATTG ATGAGAAGGA TGACAGGATC 
AAGATCCGTG GAGGGAAGCT GATGATCTCT AACACCAGGA AAAGCGACGC TGGAATGTAC 
ATCTGCGTGG GCACCAACAT GGTCGGCGAA AGAGACAGTG AAACAGCCCA GGTCACTGTG 
TTCGAGAGGC CCACGTTCCT GCGGCGTCCC ATTAACCAGG TGGTTCTGGA GGAGGAGGCT 
GTGGAATTTC GATGTCAGGT GCAGGGAGAC CCGCAGCCGT CCATCCGCTG GAAAAAAGAC 
GACGTTGAAA TCCCCCGCGG CAGGTACGAT ATGAAATTTG ATAAAGATGA CTTCACGCTG 
AGAATAAAGA AGGCAACAGC GAGTGATGAA GGAACCTTCA CCTGTGTAGC GGAGAACCGA 
GTGGGCAAAA TGGAGGCCTC CGCAACCCTC ACGGTCAGAG CTCGCCCTGT CGCTGCACCG 
CAGTTCGTGA TCAGGCCGCG GGATCAGATT GTGGCACAGG GACGCACCGC CAGCTTCCCC 
TGCGAGACTA AAGGGAACCC GCAGCCGGCA GTCTTCTGGC AGAAAGAAGG AAGTCAGAAT 
CTCCTGTTTC CAAATCAACC CCAGCAGCCC AACAGTCGTT TCTCGGTGTC GCCCAGTGGA 
GATCTAACCA TCACTACGGT CCAGCGGGCA GATGCGGGCT ATTATATCTG CCAGGCCCTG 
ACGGTGGCGG GCAGCATCCT GGCCAAAGCA CAGCTTGAGG TCACAGACGT GTTGACCGAT 
CGCCCTCCAC CCATCATCCG GCAGGGTCCA GCTAACCAGA CTCTTGGTGT AGACAGTGTG 
GCTCTTTTGA GGTGCCATGC ATCCGGAGAA CCAGTCCCAA CCATCAGCTG GCTGAAGGAC 
GGGGTCAGTC TGCTGGGCAA AGACCCTCGG ATGTCCCTGC AAGACCTCGG CAGCCTGCAG 
ATCAAGGGTT TACGGCTCTC TGATTCTGGC ATTTATACTT GTGTGGCTGC AAGTTCTAGT 
GGGGAAACAT CATGGAGTGC TTTCTTGGAG GTCAAAGAGG CTGGGAGTCT TGTTGTAGTT 
AAAGATCGGG ATGAGAACGA GCTTCCAGGA CCTCCTTCCA AACCTCAGGT CACAGATGTC 
ACTAAAAACA GCGTGTCATT ATCCTGGCAG CCAGGCCTTC CCGGAGCATC AGCCATTTCT 
ACATATGTCA TAGAAGCATT CAGCCAATCG GTGAGCAACA GCTGGCAAAC TGTTGCCGAT 
CACGCCAAGA CAACGCAGTA CACCATTAAG GGTCTCCGCC CTAACACCAT CTACCTGTTC 
ATGGTGCGAG CGGTCAACGT CCAAGGCCTG AGCGACCCAA GCCCCATGTC TGAACCCGTC 
CGCACTCAAG ATATAAGTCC TCCAGCGCAG GGTGTGGATC ACAGACATGT GCAAAAGGAG 
CTGGGAGAAG TTATTGTGCG GCTACACAAC CCTGTGGTCC TTAGCCCTAC CACCATACAG 
GTCACATGGA CGGTTGACCG CCAGTCTCAG TTCATCCAGG GCTACAGGGT TTTGTACAGG 
CAGATGTCAG GCCTGTCTTC TCCAGGAGCC TGGCAAACTC AGGATGTGAA AGTCCCCTCA 
GAGAGGAGCA TGGTGCTCTC GGCTCTAAAG AAAGGCATCG TCTACGAGAT CAAAGTGCGT 
CCATATTTCA ATGAGTTTCA GGGCATGGAC AGCGAATCCC GAACCGCAAG AACAACAGAG 
GAAGCGCCCA GTGCTCCTCC TCAGCAGGTG ACTGTACTCA CTGTGGGCAA CCAGAACAGC 
ACATCTATTA GCATCTCCTG GGACCCTCCG CCTGCAGAAC ACCAGAACGG CATCATACAG 
GAGTACAAGA TCTGGTGTTT GGGGAATGAG ACCCGTTTTC ATGTGAATAA GACGGTGGAC 
GCAGCGATCC GCTCAGTGGT GGTTGGGGGT CTCCAGGCGG GGGTCCAGTA TCGGGTGGAG 
GTGGCCGCTA GCACCAGCGC CGGGGTCGGA GTGAAGAGCG AACCACAAAC CATCATCATT 
GGTAAAGACT TCCGCGATGT GATGATTAGC AGGAATAACA GCATCACAGA GCAGATCACG 
GATGTGGTGA AACAGCCGGC CTTCATCGCG GGCATTGGCG GGGCCTGCTG GGTCATTCTG 
ATGGGCTTCA GCATCTGGCT CTACTGGAGG AGGAAGAAGA GAAAGGGACT CAGCAATTAC 
GCAGTTCAGT CGTTCACCTT CACCCCTGCA GTGACGTTCC AGCGTGTTGA TGGAGGCTTG 
ATGAGCAACG GGAGTCGTCC TGGTCTGCTG AACGCCAGTG ATTCGGGTTA CCCGTGGCTC 
GCCGACTCCT GGCCTCCCAG CCTGCCTGTC AACAGCGGAT CTGGAGGACC CAATGACCTC 
AGCTTTGGCC GTGGAGATGT GATGCCCAGT GCTCAGGCTG ATAAGACGGG TACCATGCTT 
TCGGATGGAG CCATTTACAG CAGCATCGAC TTCACCACCA AAGGCGGATT CGGTAGCCCA 
AGCCCAGCCT CCCAGGCCAC GCCCTATGCC ACCACACAGA TCCTCCACTC CAACAGCATC 
CATGAGCTGG CAGTGGATCT TCCTGAAGCC CAGTGGAAGA CCTCCCTACA GGCCAAGCAG 
GAGATGGCCA GCCTGGGTTA CTCACTGCCC GACAAAAACT CCTGCAATAA CACACTCCTT 
TTCATTCCTG ACTACCGATT GGCTGACGGA CTGTCTAATA GAATGCCACA CAACCAATCG 
CAGGATTTCA GCACCACCAG CTCCCACAAC AGCTCAGATC GGAGCAACAG TCTGTCAGGT 
GGAAAAGGTG GTAAGAAGAA GAAGACCAAA GCAAACGTCA AGCCCACCAA GATGAATGGC 
TCCAACTGGG CGAACGTTCC ACTTCCTCCT CCTCCCATCC ACCCTCTTCC TGGCACAGAG 
ATGGACCACT ACGCCTCAGA GCACCATGAT GGAGGAGGAT ATGATAGTGA TGGTTGGGGC 
CCACCATTGC CAGTGCAGAC GTACCTCCAT CAGGGTTTAG AGGATGAGCT GGAGGAAGAG 
GAAGAGCGGG TGCCAACTCC TCCCATTAGG GGTGTGGCTT CTTCTCCTGC TGCAGTTTCC 
TTTGGCAAAC AGTCCACAGC CACACTGAGC CCATCTCCAC GTGAAGAAAT GCAGCCCATG 
CTCCAAGCCC ACCTAGATGA GCTTACCCGT GCTTATCAAT TGGACATGGC TAAACAGACT 
TGGCACATGC AGGGTGGGCC TTACCCGCCC CAGGCCCCCG CACCACCCGT GGGCTACGTC 
TCCAGCACCT TAGTGTCTGA CATCGAGACC GATCTCCCTG ATGAAGATGA CGATGATGAG 
GAGGATGAAG GATACGAGAT GTCGCCACCA ATTAGGGGCA CTGAACACAC GCCAGGCTCC 
AGCATGGACA ACTTAGACAG CTCCGTTACG GATAAAGGTC TACCTCATAG ACCTCGTCCC 
GCTAGCCCGT TCTCCACAGA TGGAAGCACG GTGGGCACAC ACAGTCTGGG GCACCAGCGG 
GCAGCAAGGC CCACACGTAA ACACAAAGGC CCTGGCACTG TCCCGAACCG CCGTGATGCC 
AGTGCGGATG ATCTGCCTCC ACCTCCAGAG CCTCCACCCT GCCAGACAGC TCGCATCCCC 
GGGTCGAGGA ACGTTCCCCA TCCCTGCGAC CGCAAAGCTT CCTCTCTGGA GAGGCAGCCT 
ATGATGGGCC TTGAGGAAAT GAGAAGTTCC TTGGACCGGC AGGCCCGAAC CTCATTGGAC 
CGCCACCAAC AAGTCCAGGA CAGACTGGGG TCCATAGAGA GAGGAGATGA AAGCAGAAGA 
GGACAGAAAA ATGGTCCCTT TTCAGAAGAG GCCCTATTGC CATACAACAA ACCATCATTC 
CCATCCCCTG GTGGGCACAG CTCATCTGGG ACGGCCTCTT CTAAAGGGTC AACGGGTCCT 
CGCAAAGGAG AAGGGACGAG GGGACAGCAC CAGCGCTCCA CCACGGAACA AGGAGAAAGC 
AGCTACATGG GGACCAACGC GCAGTTTTCC GGAGAGTTAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

robo2 ( XM_021481460.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021337135.1 Danio rerio roundabout, axon guidance receptor, homolog 2 (Drosophila) (robo2), transcript variant X10, mRNA.

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CloneID	ODa148983
Clone ID Related Accession (Same CDS sequence)	XM_021481460.1
Accession Version	XM_021481460.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4749bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	roundabout homolog 2 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGTCT CCCCTGAGCG AGCGAGGTGT GCTGTGGTCC TGGCTGGACC TCCTCTCCTC 
CTCATTATCT GTGTCCTGTG GATCCTGTGC TCAGACTCAG TGGATGCTCA AGGGCAAGGA 
TCCCGTCTGC GGCAGGAAGA TTCTCCACCT CGGATTGTAG AACACCCCTC CGATCTGATC 
GTGTCAAAGG GCGAGCCAGC CACCCTAAAC TGCAAGGCGG AGGGTCGGCC AACTCCCACC 
GTGGAGTGGT ACAAGGATGG AGAACGAGTT GAAACGGACA AAGATGACCC TCGATCTCAC 
CGCATGCTCC TTCCCAGTGG TTCGCTCTTC TTCCTGCGTA TCGTACATGG ACGCCGCAGC 
AAACCTGATG AGGGGGCGTA CGTCTGTGTG GCACGCAACT ACTTGGGGGA AGCAGTCAGT 
CGAAACGCCT CGCTGGAAGT AGCATTGTTA CGGGATGATT TCCGGCAGAA CCCTACAGAT 
GTGGTGGTCG CTGCTGGAGA GCCGGCCATC CTGGAGTGTG TCCCTCCCAG AGGACACCCG 
GAGCCCACCA TCTACTGGAA GAAAGACAAA GTCCGAATTG ATGAGAAGGA TGACAGGATC 
AAGATCCGTG GAGGGAAGCT GATGATCTCT AACACCAGGA AAAGCGACGC TGGAATGTAC 
ATCTGCGTGG GCACCAACAT GGTCGGCGAA AGAGACAGTG AAACAGCCCA GGTCACTGTG 
TTCGAGAGGC CCACGTTCCT GCGGCGTCCC ATTAACCAGG TGGTTCTGGA GGAGGAGGCT 
GTGGAATTTC GATGTCAGGT GCAGGGAGAC CCGCAGCCGT CCATCCGCTG GAAAAAAGAC 
GACGTTGAAA TCCCCCGCGG CAGGTACGAT ATGAAATTTG ATAAAGATGA CTTCACGCTG 
AGAATAAAGA AGGCAACAGC GAGTGATGAA GGAACCTTCA CCTGTGTAGC GGAGAACCGA 
GTGGGCAAAA TGGAGGCCTC CGCAACCCTC ACGGTCAGAG CTGCACCGCA GTTCGTGATC 
AGGCCGCGGG ATCAGATTGT GGCACAGGGA CGCACCGCCA GCTTCCCCTG CGAGACTAAA 
GGGAACCCGC AGCCGGCAGT CTTCTGGCAG AAAGAAGGAA GTCAGAATCT CCTGTTTCCA 
AATCAACCCC AGCAGCCCAA CAGTCGTTTC TCGGTGTCGC CCAGTGGAGA TCTAACCATC 
ACTACGGTCC AGCGGGCAGA TGCGGGCTAT TATATCTGCC AGGCCCTGAC GGTGGCGGGC 
AGCATCCTGG CCAAAGCACA GCTTGAGGTC ACAGACGTGT TGACCGATCG CCCTCCACCC 
ATCATCCGGC AGGGTCCAGC TAACCAGACT CTTGGTGTAG ACAGTGTGGC TCTTTTGAGG 
TGCCATGCAT CCGGAGAACC AGTCCCAACC ATCAGCTGGC TGAAGGACGG GGTCAGTCTG 
CTGGGCAAAG ACCCTCGGAT GTCCCTGCAA GACCTCGGCA GCCTGCAGAT CAAGGGTTTA 
CGGCTCTCTG ATTCTGGCAT TTATACTTGT GTGGCTGCAA GTTCTAGTGG GGAAACATCA 
TGGAGTGCTT TCTTGGAGGT CAAAGAGGCT GGGAGTCTTG TTGTAGTTAA AGATCGGGAT 
GAGAACGAGC TTCCAGGACC TCCTTCCAAA CCTCAGGTCA CAGATGTCAC TAAAAACAGC 
GTGTCATTAT CCTGGCAGCC AGGCCTTCCC GGAGCATCAG CCATTTCTAC ATATGTCATA 
GAAGCATTCA GCCAATCGGT GAGCAACAGC TGGCAAACTG TTGCCGATCA CGCCAAGACA 
ACGCAGTACA CCATTAAGGG TCTCCGCCCT AACACCATCT ACCTGTTCAT GGTGCGAGCG 
GTCAACGTCC AAGGCCTGAG CGACCCAAGC CCCATGTCTG AACCCGTCCG CACTCAAGAT 
ATAAGTCCTC CAGCGCAGGG TGTGGATCAC AGACATGTGC AAAAGGAGCT GGGAGAAGTT 
ATTGTGCGGC TACACAACCC TGTGGTCCTT AGCCCTACCA CCATACAGGT CACATGGACG 
GTTGACCGCC AGTCTCAGTT CATCCAGGGC TACAGGGTTT TGTACAGGCA GATGTCAGGC 
CTGTCTTCTC CAGGAGCCTG GCAAACTCAG GATGTGAAAG TCCCCTCAGA GAGGAGCATG 
GTGCTCTCGG CTCTAAAGAA AGGCATCGTC TACGAGATCA AAGTGCGTCC ATATTTCAAT 
GAGTTTCAGG GCATGGACAG CGAATCCCGA ACCGCAAGAA CAACAGAGGA AGCGCCCAGT 
GCTCCTCCTC AGCAGGTGAC TGTACTCACT GTGGGCAACC AGAACAGCAC ATCTATTAGC 
ATCTCCTGGG ACCCTCCGCC TGCAGAACAC CAGAACGGCA TCATACAGGA GTACAAGATC 
TGGTGTTTGG GGAATGAGAC CCGTTTTCAT GTGAATAAGA CGGTGGACGC AGCGATCCGC 
TCAGTGGTGG TTGGGGGTCT CCAGGCGGGG GTCCAGTATC GGGTGGAGGT GGCCGCTAGC 
ACCAGCGCCG GGGTCGGAGT GAAGAGCGAA CCACAAACCA TCATCATTGG TAAAGACTTC 
CGCGATGTGA TGATTAGCAG GAATAACAGC ATCACAGAGC AGATCACGGA TGTGGTGAAA 
CAGCCGGCCT TCATCGCGGG CATTGGCGGG GCCTGCTGGG TCATTCTGAT GGGCTTCAGC 
ATCTGGCTCT ACTGGAGGAG GAAGAAGAGA AAGGGACTCA GCAATTACGC AGTGACGTTC 
CAGCGTGTTG ATGGAGGCTT GATGAGCAAC GGGAGTCGTC CTGGTCTGCT GAACGCCAGT 
GATTCGGGTT ACCCGTGGCT CGCCGACTCC TGGCCTCCCA GCCTGCCTGT CAACAGCGGA 
TCTGGAGGAC CCAATGACCT CAGCTTTGGC CGTGGAGATG TGATGCCCAG TGCTCAGGCT 
GATAAGACGG GTACCATGCT TTCGGATGGA GCCATTTACA GCAGCATCGA CTTCACCACC 
AAAGGCGGAT TCGGTAGCCC AAGCCCAGCC TCCCAGGCCA CGCCCTATGC CACCACACAG 
ATCCTCCACT CCAACAGCAT CCATGAGCTG GCAGTGGATC TTCCTGAAGC CCAGTGGAAG 
ACCTCCCTAC AGGCCAAGCA GGAGATGGCC AGCCTGGGTT ACTCACTGCC CGACAAAAAC 
TCCTGCAATA ACACACTCCT TTTCATTCCT GACTACCGAT TGGCTGACGG ACTGTCTAAT 
AGAATGCCAC ACAACCAATC GCAGGATTTC AGCACCACCA GCTCCCACAA CAGCTCAGAT 
CGGAGCAACA GTCTGTCAGG TGGAAAAGGT GGTAAGAAGA AGAAGACCAA AGCAAACGTC 
AAGCCCACCA AGATGAATGG CTCCAACTGG GCGAACGTTC CACTTCCTCC TCCTCCCATC 
CACCCTCTTC CTGGCACAGA GATGGACCAC TACGCCTCAG AGCACCATGA TGGAGGAGGA 
TATGATAGTG ATGGTTGGGG CCCACCATTG CCAGTGCAGA CGTACCTCCA TCAGGGTTTA 
GAGGATGAGC TGGAGGAAGA GGAAGAGCGG GTGCCAACTC CTCCCATTAG GGGTGTGGCT 
TCTTCTCCTG CTGCAGTTTC CTTTGGCAAA CAGTCCACAG CCACACTGAG CCCATCTCCA 
CGTGAAGAAA TGCAGCCCAT GCTCCAAGCC CACCTAGATG AGCTTACCCG TGCTTATCAA 
TTGGACATGG CTAAACAGAC TTGGCACATG CAGGGTGGGC CTTACCCGCC CCAGGCCCCC 
GCACCACCCG TGGGCTACGT CTCCAGCACC TTAGTGTCTG ACATCGAGAC CGATCTCCCT 
GATGAAGATG ACGATGATGA GGAGGATGAA GGATACGAGA TGTCGCCACC AATTAGGGGC 
ACTGAACACA CGCCAGGCTC CAGCATGGAC AACTTAGACA GCTCCGTTAC GGGCTCTATG 
GTGAACGGCT GGGGCTCGGC TTCAGAAGAT GATGACAGAA ACTTCTCCAG CAGGAGGTCT 
AGTGCCGGCA GCTCTTCTGA CGGCTCGCTC TTCACCCATT GCAGCTTTGC GCGAGCCCTG 
GTGGCAGCCG CAGACAAGGC TGGTTACCGT CTGGATGGCA CCAGCCTGAG CAAAAGAGAT 
AAAGGTCTAC CTCATAGACC TCGTCCCGCT AGCCCGTTCT CCACAGATGG AAGCACGGTG 
GGCACACACA GTCTGGGGCA CCAGCGGGCA GCAAGGCCCA CACGTAAACA CAAAGGCCCT 
GGCACTGTCC CGAACCGCCG TGATGCCAGT GCGGATGATC TGCCTCCACC TCCAGAGCCT 
CCACCCTGCC AGACAGCTCG CATCCCCGGG TCGAGGAACG TTCCCCATCC CTGCGACCGC 
AAAGCTTCCT CTCTGGAGAG GCAGCCTATG ATGGGCCTTG AGGAAATGAG AAGTTCCTTG 
GACCGGCAGG CCCGAACCTC ATTGGACCGC CACCAACAAG TCCAGGACAG ACTGGGGTCC 
ATAGAGAGAG GAGATGAAAG CAGAAGAGGA CAGAAAAATG GTCCCTTTTC AGAAGAGGCC 
CTATTGCCAT ACAACAAACC ATCATTCCCA TCCCCTGGTG GGCACAGCTC ATCTGGGACG 
GCCTCTTCTA AAGGGTCAAC GGGTCCTCGC AAAGGAGAAG GGACGAGGGG ACAGCACCAG 
CGCTCCACCA CGGAACAAGG AGAAAGCAGC TACATGGGGA CCAACGCGCA GTTTTCCGGA 
GAGTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021337135.1
CDS	252..5000
Translation	MAVSPERARCAVVLAGPPLLLIICVLWILCSDSVDAQGQGSRLRQEDSPPRIVEHPSDLIVSKGEPATLNCKAEGRPTPTVEWYKDGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRRSKPDEGAYVCVARNYLGEAVSRNASLEVALLRDDFRQNPTDVVVAAGEPAILECVPPRGHPEPTIYWKKDKVRIDEKDDRIKIRGGKLMISNTRKSDAGMYICVGTNMVGERDSETAQVTVFERPTFLRRPINQVVLEEEAVEFRCQVQGDPQPSIRWKKDDVEIPRGRYDMKFDKDDFTLRIKKATASDEGTFTCVAENRVGKMEASATLTVRAAPQFVIRPRDQIVAQGRTASFPCETKGNPQPAVFWQKEGSQNLLFPNQPQQPNSRFSVSPSGDLTITTVQRADAGYYICQALTVAGSILAKAQLEVTDVLTDRPPPIIRQGPANQTLGVDSVALLRCHASGEPVPTISWLKDGVSLLGKDPRMSLQDLGSLQIKGLRLSDSGIYTCVAASSSGETSWSAFLEVKEAGSLVVVKDRDENELPGPPSKPQVTDVTKNSVSLSWQPGLPGASAISTYVIEAFSQSVSNSWQTVADHAKTTQYTIKGLRPNTIYLFMVRAVNVQGLSDPSPMSEPVRTQDISPPAQGVDHRHVQKELGEVIVRLHNPVVLSPTTIQVTWTVDRQSQFIQGYRVLYRQMSGLSSPGAWQTQDVKVPSERSMVLSALKKGIVYEIKVRPYFNEFQGMDSESRTARTTEEAPSAPPQQVTVLTVGNQNSTSISISWDPPPAEHQNGIIQEYKIWCLGNETRFHVNKTVDAAIRSVVVGGLQAGVQYRVEVAASTSAGVGVKSEPQTIIIGKDFRDVMISRNNSITEQITDVVKQPAFIAGIGGACWVILMGFSIWLYWRRKKRKGLSNYAVTFQRVDGGLMSNGSRPGLLNASDSGYPWLADSWPPSLPVNSGSGGPNDLSFGRGDVMPSAQADKTGTMLSDGAIYSSIDFTTKGGFGSPSPASQATPYATTQILHSNSIHELAVDLPEAQWKTSLQAKQEMASLGYSLPDKNSCNNTLLFIPDYRLADGLSNRMPHNQSQDFSTTSSHNSSDRSNSLSGGKGGKKKKTKANVKPTKMNGSNWANVPLPPPPIHPLPGTEMDHYASEHHDGGGYDSDGWGPPLPVQTYLHQGLEDELEEEEERVPTPPIRGVASSPAAVSFGKQSTATLSPSPREEMQPMLQAHLDELTRAYQLDMAKQTWHMQGGPYPPQAPAPPVGYVSSTLVSDIETDLPDEDDDDEEDEGYEMSPPIRGTEHTPGSSMDNLDSSVTGSMVNGWGSASEDDDRNFSSRRSSAGSSSDGSLFTHCSFARALVAAADKAGYRLDGTSLSKRDKGLPHRPRPASPFSTDGSTVGTHSLGHQRAARPTRKHKGPGTVPNRRDASADDLPPPPEPPPCQTARIPGSRNVPHPCDRKASSLERQPMMGLEEMRSSLDRQARTSLDRHQQVQDRLGSIERGDESRRGQKNGPFSEEALLPYNKPSFPSPGGHSSSGTASSKGSTGPRKGEGTRGQHQRSTTEQGESSYMGTNAQFSGEL

Target ORF information:

RefSeq Version	XM_021481460.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio roundabout, axon guidance receptor, homolog 2 (Drosophila) (robo2), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021481460.1

ORF Insert Sequence:

ATGGCTGTCT CCCCTGAGCG AGCGAGGTGT GCTGTGGTCC TGGCTGGACC TCCTCTCCTC 
CTCATTATCT GTGTCCTGTG GATCCTGTGC TCAGACTCAG TGGATGCTCA AGGGCAAGGA 
TCCCGTCTGC GGCAGGAAGA TTCTCCACCT CGGATTGTAG AACACCCCTC CGATCTGATC 
GTGTCAAAGG GCGAGCCAGC CACCCTAAAC TGCAAGGCGG AGGGTCGGCC AACTCCCACC 
GTGGAGTGGT ACAAGGATGG AGAACGAGTT GAAACGGACA AAGATGACCC TCGATCTCAC 
CGCATGCTCC TTCCCAGTGG TTCGCTCTTC TTCCTGCGTA TCGTACATGG ACGCCGCAGC 
AAACCTGATG AGGGGGCGTA CGTCTGTGTG GCACGCAACT ACTTGGGGGA AGCAGTCAGT 
CGAAACGCCT CGCTGGAAGT AGCATTGTTA CGGGATGATT TCCGGCAGAA CCCTACAGAT 
GTGGTGGTCG CTGCTGGAGA GCCGGCCATC CTGGAGTGTG TCCCTCCCAG AGGACACCCG 
GAGCCCACCA TCTACTGGAA GAAAGACAAA GTCCGAATTG ATGAGAAGGA TGACAGGATC 
AAGATCCGTG GAGGGAAGCT GATGATCTCT AACACCAGGA AAAGCGACGC TGGAATGTAC 
ATCTGCGTGG GCACCAACAT GGTCGGCGAA AGAGACAGTG AAACAGCCCA GGTCACTGTG 
TTCGAGAGGC CCACGTTCCT GCGGCGTCCC ATTAACCAGG TGGTTCTGGA GGAGGAGGCT 
GTGGAATTTC GATGTCAGGT GCAGGGAGAC CCGCAGCCGT CCATCCGCTG GAAAAAAGAC 
GACGTTGAAA TCCCCCGCGG CAGGTACGAT ATGAAATTTG ATAAAGATGA CTTCACGCTG 
AGAATAAAGA AGGCAACAGC GAGTGATGAA GGAACCTTCA CCTGTGTAGC GGAGAACCGA 
GTGGGCAAAA TGGAGGCCTC CGCAACCCTC ACGGTCAGAG CTGCACCGCA GTTCGTGATC 
AGGCCGCGGG ATCAGATTGT GGCACAGGGA CGCACCGCCA GCTTCCCCTG CGAGACTAAA 
GGGAACCCGC AGCCGGCAGT CTTCTGGCAG AAAGAAGGAA GTCAGAATCT CCTGTTTCCA 
AATCAACCCC AGCAGCCCAA CAGTCGTTTC TCGGTGTCGC CCAGTGGAGA TCTAACCATC 
ACTACGGTCC AGCGGGCAGA TGCGGGCTAT TATATCTGCC AGGCCCTGAC GGTGGCGGGC 
AGCATCCTGG CCAAAGCACA GCTTGAGGTC ACAGACGTGT TGACCGATCG CCCTCCACCC 
ATCATCCGGC AGGGTCCAGC TAACCAGACT CTTGGTGTAG ACAGTGTGGC TCTTTTGAGG 
TGCCATGCAT CCGGAGAACC AGTCCCAACC ATCAGCTGGC TGAAGGACGG GGTCAGTCTG 
CTGGGCAAAG ACCCTCGGAT GTCCCTGCAA GACCTCGGCA GCCTGCAGAT CAAGGGTTTA 
CGGCTCTCTG ATTCTGGCAT TTATACTTGT GTGGCTGCAA GTTCTAGTGG GGAAACATCA 
TGGAGTGCTT TCTTGGAGGT CAAAGAGGCT GGGAGTCTTG TTGTAGTTAA AGATCGGGAT 
GAGAACGAGC TTCCAGGACC TCCTTCCAAA CCTCAGGTCA CAGATGTCAC TAAAAACAGC 
GTGTCATTAT CCTGGCAGCC AGGCCTTCCC GGAGCATCAG CCATTTCTAC ATATGTCATA 
GAAGCATTCA GCCAATCGGT GAGCAACAGC TGGCAAACTG TTGCCGATCA CGCCAAGACA 
ACGCAGTACA CCATTAAGGG TCTCCGCCCT AACACCATCT ACCTGTTCAT GGTGCGAGCG 
GTCAACGTCC AAGGCCTGAG CGACCCAAGC CCCATGTCTG AACCCGTCCG CACTCAAGAT 
ATAAGTCCTC CAGCGCAGGG TGTGGATCAC AGACATGTGC AAAAGGAGCT GGGAGAAGTT 
ATTGTGCGGC TACACAACCC TGTGGTCCTT AGCCCTACCA CCATACAGGT CACATGGACG 
GTTGACCGCC AGTCTCAGTT CATCCAGGGC TACAGGGTTT TGTACAGGCA GATGTCAGGC 
CTGTCTTCTC CAGGAGCCTG GCAAACTCAG GATGTGAAAG TCCCCTCAGA GAGGAGCATG 
GTGCTCTCGG CTCTAAAGAA AGGCATCGTC TACGAGATCA AAGTGCGTCC ATATTTCAAT 
GAGTTTCAGG GCATGGACAG CGAATCCCGA ACCGCAAGAA CAACAGAGGA AGCGCCCAGT 
GCTCCTCCTC AGCAGGTGAC TGTACTCACT GTGGGCAACC AGAACAGCAC ATCTATTAGC 
ATCTCCTGGG ACCCTCCGCC TGCAGAACAC CAGAACGGCA TCATACAGGA GTACAAGATC 
TGGTGTTTGG GGAATGAGAC CCGTTTTCAT GTGAATAAGA CGGTGGACGC AGCGATCCGC 
TCAGTGGTGG TTGGGGGTCT CCAGGCGGGG GTCCAGTATC GGGTGGAGGT GGCCGCTAGC 
ACCAGCGCCG GGGTCGGAGT GAAGAGCGAA CCACAAACCA TCATCATTGG TAAAGACTTC 
CGCGATGTGA TGATTAGCAG GAATAACAGC ATCACAGAGC AGATCACGGA TGTGGTGAAA 
CAGCCGGCCT TCATCGCGGG CATTGGCGGG GCCTGCTGGG TCATTCTGAT GGGCTTCAGC 
ATCTGGCTCT ACTGGAGGAG GAAGAAGAGA AAGGGACTCA GCAATTACGC AGTGACGTTC 
CAGCGTGTTG ATGGAGGCTT GATGAGCAAC GGGAGTCGTC CTGGTCTGCT GAACGCCAGT 
GATTCGGGTT ACCCGTGGCT CGCCGACTCC TGGCCTCCCA GCCTGCCTGT CAACAGCGGA 
TCTGGAGGAC CCAATGACCT CAGCTTTGGC CGTGGAGATG TGATGCCCAG TGCTCAGGCT 
GATAAGACGG GTACCATGCT TTCGGATGGA GCCATTTACA GCAGCATCGA CTTCACCACC 
AAAGGCGGAT TCGGTAGCCC AAGCCCAGCC TCCCAGGCCA CGCCCTATGC CACCACACAG 
ATCCTCCACT CCAACAGCAT CCATGAGCTG GCAGTGGATC TTCCTGAAGC CCAGTGGAAG 
ACCTCCCTAC AGGCCAAGCA GGAGATGGCC AGCCTGGGTT ACTCACTGCC CGACAAAAAC 
TCCTGCAATA ACACACTCCT TTTCATTCCT GACTACCGAT TGGCTGACGG ACTGTCTAAT 
AGAATGCCAC ACAACCAATC GCAGGATTTC AGCACCACCA GCTCCCACAA CAGCTCAGAT 
CGGAGCAACA GTCTGTCAGG TGGAAAAGGT GGTAAGAAGA AGAAGACCAA AGCAAACGTC 
AAGCCCACCA AGATGAATGG CTCCAACTGG GCGAACGTTC CACTTCCTCC TCCTCCCATC 
CACCCTCTTC CTGGCACAGA GATGGACCAC TACGCCTCAG AGCACCATGA TGGAGGAGGA 
TATGATAGTG ATGGTTGGGG CCCACCATTG CCAGTGCAGA CGTACCTCCA TCAGGGTTTA 
GAGGATGAGC TGGAGGAAGA GGAAGAGCGG GTGCCAACTC CTCCCATTAG GGGTGTGGCT 
TCTTCTCCTG CTGCAGTTTC CTTTGGCAAA CAGTCCACAG CCACACTGAG CCCATCTCCA 
CGTGAAGAAA TGCAGCCCAT GCTCCAAGCC CACCTAGATG AGCTTACCCG TGCTTATCAA 
TTGGACATGG CTAAACAGAC TTGGCACATG CAGGGTGGGC CTTACCCGCC CCAGGCCCCC 
GCACCACCCG TGGGCTACGT CTCCAGCACC TTAGTGTCTG ACATCGAGAC CGATCTCCCT 
GATGAAGATG ACGATGATGA GGAGGATGAA GGATACGAGA TGTCGCCACC AATTAGGGGC 
ACTGAACACA CGCCAGGCTC CAGCATGGAC AACTTAGACA GCTCCGTTAC GGGCTCTATG 
GTGAACGGCT GGGGCTCGGC TTCAGAAGAT GATGACAGAA ACTTCTCCAG CAGGAGGTCT 
AGTGCCGGCA GCTCTTCTGA CGGCTCGCTC TTCACCCATT GCAGCTTTGC GCGAGCCCTG 
GTGGCAGCCG CAGACAAGGC TGGTTACCGT CTGGATGGCA CCAGCCTGAG CAAAAGAGAT 
AAAGGTCTAC CTCATAGACC TCGTCCCGCT AGCCCGTTCT CCACAGATGG AAGCACGGTG 
GGCACACACA GTCTGGGGCA CCAGCGGGCA GCAAGGCCCA CACGTAAACA CAAAGGCCCT 
GGCACTGTCC CGAACCGCCG TGATGCCAGT GCGGATGATC TGCCTCCACC TCCAGAGCCT 
CCACCCTGCC AGACAGCTCG CATCCCCGGG TCGAGGAACG TTCCCCATCC CTGCGACCGC 
AAAGCTTCCT CTCTGGAGAG GCAGCCTATG ATGGGCCTTG AGGAAATGAG AAGTTCCTTG 
GACCGGCAGG CCCGAACCTC ATTGGACCGC CACCAACAAG TCCAGGACAG ACTGGGGTCC 
ATAGAGAGAG GAGATGAAAG CAGAAGAGGA CAGAAAAATG GTCCCTTTTC AGAAGAGGCC 
CTATTGCCAT ACAACAAACC ATCATTCCCA TCCCCTGGTG GGCACAGCTC ATCTGGGACG 
GCCTCTTCTA AAGGGTCAAC GGGTCCTCGC AAAGGAGAAG GGACGAGGGG ACAGCACCAG 
CGCTCCACCA CGGAACAAGG AGAAAGCAGC TACATGGGGA CCAACGCGCA GTTTTCCGGA 
GAGTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

robo2 ( XM_021481461.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021337136.1 Danio rerio roundabout, axon guidance receptor, homolog 2 (Drosophila) (robo2), transcript variant X11, mRNA.

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CloneID	ODa148984
Clone ID Related Accession (Same CDS sequence)	XM_021481461.1
Accession Version	XM_021481461.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4623bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	roundabout homolog 2 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGTCT CCCCTGAGCG AGCGAGGTGT GCTGTGGTCC TGGCTGGACC TCCTCTCCTC 
CTCATTATCT GTGTCCTGTG GATCCTGTGC TCAGACTCAG TGGATGCTCA AGGGCAAGGA 
TCCCGTCTGC GGCAGGAAGA TTCTCCACCT CGGATTGTAG AACACCCCTC CGATCTGATC 
GTGTCAAAGG GCGAGCCAGC CACCCTAAAC TGCAAGGCGG AGGGTCGGCC AACTCCCACC 
GTGGAGTGGT ACAAGGATGG AGAACGAGTT GAAACGGACA AAGATGACCC TCGATCTCAC 
CGCATGCTCC TTCCCAGTGG TTCGCTCTTC TTCCTGCGTA TCGTACATGG ACGCCGCAGC 
AAACCTGATG AGGGGGCGTA CGTCTGTGTG GCACGCAACT ACTTGGGGGA AGCAGTCAGT 
CGAAACGCCT CGCTGGAAGT AGCATTGTTA CGGGATGATT TCCGGCAGAA CCCTACAGAT 
GTGGTGGTCG CTGCTGGAGA GCCGGCCATC CTGGAGTGTG TCCCTCCCAG AGGACACCCG 
GAGCCCACCA TCTACTGGAA GAAAGACAAA GTCCGAATTG ATGAGAAGGA TGACAGGATC 
AAGATCCGTG GAGGGAAGCT GATGATCTCT AACACCAGGA AAAGCGACGC TGGAATGTAC 
ATCTGCGTGG GCACCAACAT GGTCGGCGAA AGAGACAGTG AAACAGCCCA GGTCACTGTG 
TTCGAGAGGC CCACGTTCCT GCGGCGTCCC ATTAACCAGG TGGTTCTGGA GGAGGAGGCT 
GTGGAATTTC GATGTCAGGT GCAGGGAGAC CCGCAGCCGT CCATCCGCTG GAAAAAAGAC 
GACGTTGAAA TCCCCCGCGG CAGGTACGAT ATGAAATTTG ATAAAGATGA CTTCACGCTG 
AGAATAAAGA AGGCAACAGC GAGTGATGAA GGAACCTTCA CCTGTGTAGC GGAGAACCGA 
GTGGGCAAAA TGGAGGCCTC CGCAACCCTC ACGGTCAGAG CTGCACCGCA GTTCGTGATC 
AGGCCGCGGG ATCAGATTGT GGCACAGGGA CGCACCGCCA GCTTCCCCTG CGAGACTAAA 
GGGAACCCGC AGCCGGCAGT CTTCTGGCAG AAAGAAGGAA GTCAGAATCT CCTGTTTCCA 
AATCAACCCC AGCAGCCCAA CAGTCGTTTC TCGGTGTCGC CCAGTGGAGA TCTAACCATC 
ACTACGGTCC AGCGGGCAGA TGCGGGCTAT TATATCTGCC AGGCCCTGAC GGTGGCGGGC 
AGCATCCTGG CCAAAGCACA GCTTGAGGTC ACAGACGTGT TGACCGATCG CCCTCCACCC 
ATCATCCGGC AGGGTCCAGC TAACCAGACT CTTGGTGTAG ACAGTGTGGC TCTTTTGAGG 
TGCCATGCAT CCGGAGAACC AGTCCCAACC ATCAGCTGGC TGAAGGACGG GGTCAGTCTG 
CTGGGCAAAG ACCCTCGGAT GTCCCTGCAA GACCTCGGCA GCCTGCAGAT CAAGGGTTTA 
CGGCTCTCTG ATTCTGGCAT TTATACTTGT GTGGCTGCAA GTTCTAGTGG GGAAACATCA 
TGGAGTGCTT TCTTGGAGGT CAAAGAGGCT GGGAGTCTTG TTGTAGTTAA AGATCGGGAT 
GAGAACGAGC TTCCAGGACC TCCTTCCAAA CCTCAGGTCA CAGATGTCAC TAAAAACAGC 
GTGTCATTAT CCTGGCAGCC AGGCCTTCCC GGAGCATCAG CCATTTCTAC ATATGTCATA 
GAAGCATTCA GCCAATCGGT GAGCAACAGC TGGCAAACTG TTGCCGATCA CGCCAAGACA 
ACGCAGTACA CCATTAAGGG TCTCCGCCCT AACACCATCT ACCTGTTCAT GGTGCGAGCG 
GTCAACGTCC AAGGCCTGAG CGACCCAAGC CCCATGTCTG AACCCGTCCG CACTCAAGAT 
ATAAGTCCTC CAGCGCAGGG TGTGGATCAC AGACATGTGC AAAAGGAGCT GGGAGAAGTT 
ATTGTGCGGC TACACAACCC TGTGGTCCTT AGCCCTACCA CCATACAGGT CACATGGACG 
GTTGACCGCC AGTCTCAGTT CATCCAGGGC TACAGGGTTT TGTACAGGCA GATGTCAGGC 
CTGTCTTCTC CAGGAGCCTG GCAAACTCAG GATGTGAAAG TCCCCTCAGA GAGGAGCATG 
GTGCTCTCGG CTCTAAAGAA AGGCATCGTC TACGAGATCA AAGTGCGTCC ATATTTCAAT 
GAGTTTCAGG GCATGGACAG CGAATCCCGA ACCGCAAGAA CAACAGAGGA AGCGCCCAGT 
GCTCCTCCTC AGCAGGTGAC TGTACTCACT GTGGGCAACC AGAACAGCAC ATCTATTAGC 
ATCTCCTGGG ACCCTCCGCC TGCAGAACAC CAGAACGGCA TCATACAGGA GTACAAGATC 
TGGTGTTTGG GGAATGAGAC CCGTTTTCAT GTGAATAAGA CGGTGGACGC AGCGATCCGC 
TCAGTGGTGG TTGGGGGTCT CCAGGCGGGG GTCCAGTATC GGGTGGAGGT GGCCGCTAGC 
ACCAGCGCCG GGGTCGGAGT GAAGAGCGAA CCACAAACCA TCATCATTGG TAAAGACTTC 
CGCGATGTGA TGATTAGCAG GAATAACAGC ATCACAGAGC AGATCACGGA TGTGGTGAAA 
CAGCCGGCCT TCATCGCGGG CATTGGCGGG GCCTGCTGGG TCATTCTGAT GGGCTTCAGC 
ATCTGGCTCT ACTGGAGGAG GAAGAAGAGA AAGGGACTCA GCAATTACGC AGTGACGTTC 
CAGCGTGTTG ATGGAGGCTT GATGAGCAAC GGGAGTCGTC CTGGTCTGCT GAACGCCAGT 
GATTCGGGTT ACCCGTGGCT CGCCGACTCC TGGCCTCCCA GCCTGCCTGT CAACAGCGGA 
TCTGGAGGAC CCAATGACCT CAGCTTTGGC CGTGGAGATG TGATGCCCAG TGCTCAGGCT 
GATAAGACGG GTACCATGCT TTCGGATGGA GCCATTTACA GCAGCATCGA CTTCACCACC 
AAAGGCGGAT TCGGTAGCCC AAGCCCAGCC TCCCAGGCCA CGCCCTATGC CACCACACAG 
ATCCTCCACT CCAACAGCAT CCATGAGCTG GCAGTGGATC TTCCTGAAGC CCAGTGGAAG 
ACCTCCCTAC AGGCCAAGCA GGAGATGGCC AGCCTGGGTT ACTCACTGCC CGACAAAAAC 
TCCTGCAATA ACAGTGGAAA AGGTGGTAAG AAGAAGAAGA CCAAAGCAAA CGTCAAGCCC 
ACCAAGATGA ATGGCTCCAA CTGGGCGAAC GTTCCACTTC CTCCTCCTCC CATCCACCCT 
CTTCCTGGCA CAGAGATGGA CCACTACGCC TCAGAGCACC ATGATGGAGG AGGATATGAT 
AGTGATGGTT GGGGCCCACC ATTGCCAGTG CAGACGTACC TCCATCAGGG TTTAGAGGAT 
GAGCTGGAGG AAGAGGAAGA GCGGGTGCCA ACTCCTCCCA TTAGGGGTGT GGCTTCTTCT 
CCTGCTGCAG TTTCCTTTGG CAAACAGTCC ACAGCCACAC TGAGCCCATC TCCACGTGAA 
GAAATGCAGC CCATGCTCCA AGCCCACCTA GATGAGCTTA CCCGTGCTTA TCAATTGGAC 
ATGGCTAAAC AGACTTGGCA CATGCAGGGT GGGCCTTACC CGCCCCAGGC CCCCGCACCA 
CCCGTGGGCT ACGTCTCCAG CACCTTAGTG TCTGACATCG AGACCGATCT CCCTGATGAA 
GATGACGATG ATGAGGAGGA TGAAGGATAC GAGATGTCGC CACCAATTAG GGGCACTGAA 
CACACGCCAG GCTCCAGCAT GGACAACTTA GACAGCTCCG TTACGGGCTC TATGGTGAAC 
GGCTGGGGCT CGGCTTCAGA AGATGATGAC AGAAACTTCT CCAGCAGGAG GTCTAGTGCC 
GGCAGCTCTT CTGACGGCTC GCTCTTCACC CATTGCAGCT TTGCGCGAGC CCTGGTGGCA 
GCCGCAGACA AGGCTGGTTA CCGTCTGGAT GGCACCAGCC TGAGCAAAAG AGATAAAGGT 
CTACCTCATA GACCTCGTCC CGCTAGCCCG TTCTCCACAG ATGGAAGCAC GGTGGGCACA 
CACAGTCTGG GGCACCAGCG GGCAGCAAGG CCCACACGTA AACACAAAGG CCCTGGCACT 
GTCCCGAACC GCCGTGATGC CAGTGCGGAT GATCTGCCTC CACCTCCAGA GCCTCCACCC 
TGCCAGACAG CTCGCATCCC CGGGTCGAGG AACGTTCCCC ATCCCTGCGA CCGCAAAGCT 
TCCTCTCTGG AGAGGCAGCC TATGATGGGC CTTGAGGAAA TGAGAAGTTC CTTGGACCGG 
CAGGCCCGAA CCTCATTGGA CCGCCACCAA CAAGTCCAGG ACAGACTGGG GTCCATAGAG 
AGAGGAGATG AAAGCAGAAG AGGACAGAAA AATGGTCCCT TTTCAGAAGA GGCCCTATTG 
CCATACAACA AACCATCATT CCCATCCCCT GGTGGGCACA GCTCATCTGG GACGGCCTCT 
TCTAAAGGGT CAACGGGTCC TCGCAAAGGA GAAGGGACGA GGGGACAGCA CCAGCGCTCC 
ACCACGGAAC AAGGAGAAAG CAGCTACATG GGGACCAACG CGCAGTTTTC CGGAGAGTTA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021337136.1
CDS	252..4874
Translation	MAVSPERARCAVVLAGPPLLLIICVLWILCSDSVDAQGQGSRLRQEDSPPRIVEHPSDLIVSKGEPATLNCKAEGRPTPTVEWYKDGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRRSKPDEGAYVCVARNYLGEAVSRNASLEVALLRDDFRQNPTDVVVAAGEPAILECVPPRGHPEPTIYWKKDKVRIDEKDDRIKIRGGKLMISNTRKSDAGMYICVGTNMVGERDSETAQVTVFERPTFLRRPINQVVLEEEAVEFRCQVQGDPQPSIRWKKDDVEIPRGRYDMKFDKDDFTLRIKKATASDEGTFTCVAENRVGKMEASATLTVRAAPQFVIRPRDQIVAQGRTASFPCETKGNPQPAVFWQKEGSQNLLFPNQPQQPNSRFSVSPSGDLTITTVQRADAGYYICQALTVAGSILAKAQLEVTDVLTDRPPPIIRQGPANQTLGVDSVALLRCHASGEPVPTISWLKDGVSLLGKDPRMSLQDLGSLQIKGLRLSDSGIYTCVAASSSGETSWSAFLEVKEAGSLVVVKDRDENELPGPPSKPQVTDVTKNSVSLSWQPGLPGASAISTYVIEAFSQSVSNSWQTVADHAKTTQYTIKGLRPNTIYLFMVRAVNVQGLSDPSPMSEPVRTQDISPPAQGVDHRHVQKELGEVIVRLHNPVVLSPTTIQVTWTVDRQSQFIQGYRVLYRQMSGLSSPGAWQTQDVKVPSERSMVLSALKKGIVYEIKVRPYFNEFQGMDSESRTARTTEEAPSAPPQQVTVLTVGNQNSTSISISWDPPPAEHQNGIIQEYKIWCLGNETRFHVNKTVDAAIRSVVVGGLQAGVQYRVEVAASTSAGVGVKSEPQTIIIGKDFRDVMISRNNSITEQITDVVKQPAFIAGIGGACWVILMGFSIWLYWRRKKRKGLSNYAVTFQRVDGGLMSNGSRPGLLNASDSGYPWLADSWPPSLPVNSGSGGPNDLSFGRGDVMPSAQADKTGTMLSDGAIYSSIDFTTKGGFGSPSPASQATPYATTQILHSNSIHELAVDLPEAQWKTSLQAKQEMASLGYSLPDKNSCNNSGKGGKKKKTKANVKPTKMNGSNWANVPLPPPPIHPLPGTEMDHYASEHHDGGGYDSDGWGPPLPVQTYLHQGLEDELEEEEERVPTPPIRGVASSPAAVSFGKQSTATLSPSPREEMQPMLQAHLDELTRAYQLDMAKQTWHMQGGPYPPQAPAPPVGYVSSTLVSDIETDLPDEDDDDEEDEGYEMSPPIRGTEHTPGSSMDNLDSSVTGSMVNGWGSASEDDDRNFSSRRSSAGSSSDGSLFTHCSFARALVAAADKAGYRLDGTSLSKRDKGLPHRPRPASPFSTDGSTVGTHSLGHQRAARPTRKHKGPGTVPNRRDASADDLPPPPEPPPCQTARIPGSRNVPHPCDRKASSLERQPMMGLEEMRSSLDRQARTSLDRHQQVQDRLGSIERGDESRRGQKNGPFSEEALLPYNKPSFPSPGGHSSSGTASSKGSTGPRKGEGTRGQHQRSTTEQGESSYMGTNAQFSGEL

Target ORF information:

RefSeq Version	XM_021481461.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio roundabout, axon guidance receptor, homolog 2 (Drosophila) (robo2), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021481461.1

ORF Insert Sequence:

ATGGCTGTCT CCCCTGAGCG AGCGAGGTGT GCTGTGGTCC TGGCTGGACC TCCTCTCCTC 
CTCATTATCT GTGTCCTGTG GATCCTGTGC TCAGACTCAG TGGATGCTCA AGGGCAAGGA 
TCCCGTCTGC GGCAGGAAGA TTCTCCACCT CGGATTGTAG AACACCCCTC CGATCTGATC 
GTGTCAAAGG GCGAGCCAGC CACCCTAAAC TGCAAGGCGG AGGGTCGGCC AACTCCCACC 
GTGGAGTGGT ACAAGGATGG AGAACGAGTT GAAACGGACA AAGATGACCC TCGATCTCAC 
CGCATGCTCC TTCCCAGTGG TTCGCTCTTC TTCCTGCGTA TCGTACATGG ACGCCGCAGC 
AAACCTGATG AGGGGGCGTA CGTCTGTGTG GCACGCAACT ACTTGGGGGA AGCAGTCAGT 
CGAAACGCCT CGCTGGAAGT AGCATTGTTA CGGGATGATT TCCGGCAGAA CCCTACAGAT 
GTGGTGGTCG CTGCTGGAGA GCCGGCCATC CTGGAGTGTG TCCCTCCCAG AGGACACCCG 
GAGCCCACCA TCTACTGGAA GAAAGACAAA GTCCGAATTG ATGAGAAGGA TGACAGGATC 
AAGATCCGTG GAGGGAAGCT GATGATCTCT AACACCAGGA AAAGCGACGC TGGAATGTAC 
ATCTGCGTGG GCACCAACAT GGTCGGCGAA AGAGACAGTG AAACAGCCCA GGTCACTGTG 
TTCGAGAGGC CCACGTTCCT GCGGCGTCCC ATTAACCAGG TGGTTCTGGA GGAGGAGGCT 
GTGGAATTTC GATGTCAGGT GCAGGGAGAC CCGCAGCCGT CCATCCGCTG GAAAAAAGAC 
GACGTTGAAA TCCCCCGCGG CAGGTACGAT ATGAAATTTG ATAAAGATGA CTTCACGCTG 
AGAATAAAGA AGGCAACAGC GAGTGATGAA GGAACCTTCA CCTGTGTAGC GGAGAACCGA 
GTGGGCAAAA TGGAGGCCTC CGCAACCCTC ACGGTCAGAG CTGCACCGCA GTTCGTGATC 
AGGCCGCGGG ATCAGATTGT GGCACAGGGA CGCACCGCCA GCTTCCCCTG CGAGACTAAA 
GGGAACCCGC AGCCGGCAGT CTTCTGGCAG AAAGAAGGAA GTCAGAATCT CCTGTTTCCA 
AATCAACCCC AGCAGCCCAA CAGTCGTTTC TCGGTGTCGC CCAGTGGAGA TCTAACCATC 
ACTACGGTCC AGCGGGCAGA TGCGGGCTAT TATATCTGCC AGGCCCTGAC GGTGGCGGGC 
AGCATCCTGG CCAAAGCACA GCTTGAGGTC ACAGACGTGT TGACCGATCG CCCTCCACCC 
ATCATCCGGC AGGGTCCAGC TAACCAGACT CTTGGTGTAG ACAGTGTGGC TCTTTTGAGG 
TGCCATGCAT CCGGAGAACC AGTCCCAACC ATCAGCTGGC TGAAGGACGG GGTCAGTCTG 
CTGGGCAAAG ACCCTCGGAT GTCCCTGCAA GACCTCGGCA GCCTGCAGAT CAAGGGTTTA 
CGGCTCTCTG ATTCTGGCAT TTATACTTGT GTGGCTGCAA GTTCTAGTGG GGAAACATCA 
TGGAGTGCTT TCTTGGAGGT CAAAGAGGCT GGGAGTCTTG TTGTAGTTAA AGATCGGGAT 
GAGAACGAGC TTCCAGGACC TCCTTCCAAA CCTCAGGTCA CAGATGTCAC TAAAAACAGC 
GTGTCATTAT CCTGGCAGCC AGGCCTTCCC GGAGCATCAG CCATTTCTAC ATATGTCATA 
GAAGCATTCA GCCAATCGGT GAGCAACAGC TGGCAAACTG TTGCCGATCA CGCCAAGACA 
ACGCAGTACA CCATTAAGGG TCTCCGCCCT AACACCATCT ACCTGTTCAT GGTGCGAGCG 
GTCAACGTCC AAGGCCTGAG CGACCCAAGC CCCATGTCTG AACCCGTCCG CACTCAAGAT 
ATAAGTCCTC CAGCGCAGGG TGTGGATCAC AGACATGTGC AAAAGGAGCT GGGAGAAGTT 
ATTGTGCGGC TACACAACCC TGTGGTCCTT AGCCCTACCA CCATACAGGT CACATGGACG 
GTTGACCGCC AGTCTCAGTT CATCCAGGGC TACAGGGTTT TGTACAGGCA GATGTCAGGC 
CTGTCTTCTC CAGGAGCCTG GCAAACTCAG GATGTGAAAG TCCCCTCAGA GAGGAGCATG 
GTGCTCTCGG CTCTAAAGAA AGGCATCGTC TACGAGATCA AAGTGCGTCC ATATTTCAAT 
GAGTTTCAGG GCATGGACAG CGAATCCCGA ACCGCAAGAA CAACAGAGGA AGCGCCCAGT 
GCTCCTCCTC AGCAGGTGAC TGTACTCACT GTGGGCAACC AGAACAGCAC ATCTATTAGC 
ATCTCCTGGG ACCCTCCGCC TGCAGAACAC CAGAACGGCA TCATACAGGA GTACAAGATC 
TGGTGTTTGG GGAATGAGAC CCGTTTTCAT GTGAATAAGA CGGTGGACGC AGCGATCCGC 
TCAGTGGTGG TTGGGGGTCT CCAGGCGGGG GTCCAGTATC GGGTGGAGGT GGCCGCTAGC 
ACCAGCGCCG GGGTCGGAGT GAAGAGCGAA CCACAAACCA TCATCATTGG TAAAGACTTC 
CGCGATGTGA TGATTAGCAG GAATAACAGC ATCACAGAGC AGATCACGGA TGTGGTGAAA 
CAGCCGGCCT TCATCGCGGG CATTGGCGGG GCCTGCTGGG TCATTCTGAT GGGCTTCAGC 
ATCTGGCTCT ACTGGAGGAG GAAGAAGAGA AAGGGACTCA GCAATTACGC AGTGACGTTC 
CAGCGTGTTG ATGGAGGCTT GATGAGCAAC GGGAGTCGTC CTGGTCTGCT GAACGCCAGT 
GATTCGGGTT ACCCGTGGCT CGCCGACTCC TGGCCTCCCA GCCTGCCTGT CAACAGCGGA 
TCTGGAGGAC CCAATGACCT CAGCTTTGGC CGTGGAGATG TGATGCCCAG TGCTCAGGCT 
GATAAGACGG GTACCATGCT TTCGGATGGA GCCATTTACA GCAGCATCGA CTTCACCACC 
AAAGGCGGAT TCGGTAGCCC AAGCCCAGCC TCCCAGGCCA CGCCCTATGC CACCACACAG 
ATCCTCCACT CCAACAGCAT CCATGAGCTG GCAGTGGATC TTCCTGAAGC CCAGTGGAAG 
ACCTCCCTAC AGGCCAAGCA GGAGATGGCC AGCCTGGGTT ACTCACTGCC CGACAAAAAC 
TCCTGCAATA ACAGTGGAAA AGGTGGTAAG AAGAAGAAGA CCAAAGCAAA CGTCAAGCCC 
ACCAAGATGA ATGGCTCCAA CTGGGCGAAC GTTCCACTTC CTCCTCCTCC CATCCACCCT 
CTTCCTGGCA CAGAGATGGA CCACTACGCC TCAGAGCACC ATGATGGAGG AGGATATGAT 
AGTGATGGTT GGGGCCCACC ATTGCCAGTG CAGACGTACC TCCATCAGGG TTTAGAGGAT 
GAGCTGGAGG AAGAGGAAGA GCGGGTGCCA ACTCCTCCCA TTAGGGGTGT GGCTTCTTCT 
CCTGCTGCAG TTTCCTTTGG CAAACAGTCC ACAGCCACAC TGAGCCCATC TCCACGTGAA 
GAAATGCAGC CCATGCTCCA AGCCCACCTA GATGAGCTTA CCCGTGCTTA TCAATTGGAC 
ATGGCTAAAC AGACTTGGCA CATGCAGGGT GGGCCTTACC CGCCCCAGGC CCCCGCACCA 
CCCGTGGGCT ACGTCTCCAG CACCTTAGTG TCTGACATCG AGACCGATCT CCCTGATGAA 
GATGACGATG ATGAGGAGGA TGAAGGATAC GAGATGTCGC CACCAATTAG GGGCACTGAA 
CACACGCCAG GCTCCAGCAT GGACAACTTA GACAGCTCCG TTACGGGCTC TATGGTGAAC 
GGCTGGGGCT CGGCTTCAGA AGATGATGAC AGAAACTTCT CCAGCAGGAG GTCTAGTGCC 
GGCAGCTCTT CTGACGGCTC GCTCTTCACC CATTGCAGCT TTGCGCGAGC CCTGGTGGCA 
GCCGCAGACA AGGCTGGTTA CCGTCTGGAT GGCACCAGCC TGAGCAAAAG AGATAAAGGT 
CTACCTCATA GACCTCGTCC CGCTAGCCCG TTCTCCACAG ATGGAAGCAC GGTGGGCACA 
CACAGTCTGG GGCACCAGCG GGCAGCAAGG CCCACACGTA AACACAAAGG CCCTGGCACT 
GTCCCGAACC GCCGTGATGC CAGTGCGGAT GATCTGCCTC CACCTCCAGA GCCTCCACCC 
TGCCAGACAG CTCGCATCCC CGGGTCGAGG AACGTTCCCC ATCCCTGCGA CCGCAAAGCT 
TCCTCTCTGG AGAGGCAGCC TATGATGGGC CTTGAGGAAA TGAGAAGTTC CTTGGACCGG 
CAGGCCCGAA CCTCATTGGA CCGCCACCAA CAAGTCCAGG ACAGACTGGG GTCCATAGAG 
AGAGGAGATG AAAGCAGAAG AGGACAGAAA AATGGTCCCT TTTCAGAAGA GGCCCTATTG 
CCATACAACA AACCATCATT CCCATCCCCT GGTGGGCACA GCTCATCTGG GACGGCCTCT 
TCTAAAGGGT CAACGGGTCC TCGCAAAGGA GAAGGGACGA GGGGACAGCA CCAGCGCTCC 
ACCACGGAAC AAGGAGAAAG CAGCTACATG GGGACCAACG CGCAGTTTTC CGGAGAGTTA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

robo2 ( XM_017350998.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017206487.1 Danio rerio roundabout, axon guidance receptor, homolog 2 (Drosophila) (robo2), transcript variant X1, mRNA.

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CloneID	ODa55117
Clone ID Related Accession (Same CDS sequence)	XM_017350998.1
Accession Version	XM_017350998.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4800bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	roundabout homolog 2 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCCTCC TCTCTCCTGT GAGACTCACT TTATTTCTGT TGGTCTTCCT GGATGTTCCT 
CATCCGTCCC TTCAGGGCAG GAATAAGGGC AGATGGAACA AGGGGGGATC CCGTCTGCGG 
CAGGAAGATT CTCCACCTCG GATTGTAGAA CACCCCTCCG ATCTGATCGT GTCAAAGGGC 
GAGCCAGCCA CCCTAAACTG CAAGGCGGAG GGTCGGCCAA CTCCCACCGT GGAGTGGTAC 
AAGGATGGAG AACGAGTTGA AACGGACAAA GATGACCCTC GATCTCACCG CATGCTCCTT 
CCCAGTGGTT CGCTCTTCTT CCTGCGTATC GTACATGGAC GCCGCAGCAA ACCTGATGAG 
GGGGCGTACG TCTGTGTGGC ACGCAACTAC TTGGGGGAAG CAGTCAGTCG AAACGCCTCG 
CTGGAAGTAG CATTGTTACG GGATGATTTC CGGCAGAACC CTACAGATGT GGTGGTCGCT 
GCTGGAGAGC CGGCCATCCT GGAGTGTGTC CCTCCCAGAG GACACCCGGA GCCCACCATC 
TACTGGAAGA AAGACAAAGT CCGAATTGAT GAGAAGGATG ACAGGATCAA GATCCGTGGA 
GGGAAGCTGA TGATCTCTAA CACCAGGAAA AGCGACGCTG GAATGTACAT CTGCGTGGGC 
ACCAACATGG TCGGCGAAAG AGACAGTGAA ACAGCCCAGG TCACTGTGTT CGAGAGGCCC 
ACGTTCCTGC GGCGTCCCAT TAACCAGGTG GTTCTGGAGG AGGAGGCTGT GGAATTTCGA 
TGTCAGGTGC AGGGAGACCC GCAGCCGTCC ATCCGCTGGA AAAAAGACGA CGTTGAAATC 
CCCCGCGGCA GGTACGATAT GAAATTTGAT AAAGATGACT TCACGCTGAG AATAAAGAAG 
GCAACAGCGA GTGATGAAGG AACCTTCACC TGTGTAGCGG AGAACCGAGT GGGCAAAATG 
GAGGCCTCCG CAACCCTCAC GGTCAGAGCT CGCCCTGTCG CTGCACCGCA GTTCGTGATC 
AGGCCGCGGG ATCAGATTGT GGCACAGGGA CGCACCGCCA GCTTCCCCTG CGAGACTAAA 
GGGAACCCGC AGCCGGCAGT CTTCTGGCAG AAAGAAGGAA GTCAGAATCT CCTGTTTCCA 
AATCAACCCC AGCAGCCCAA CAGTCGTTTC TCGGTGTCGC CCAGTGGAGA TCTAACCATC 
ACTACGGTCC AGCGGGCAGA TGCGGGCTAT TATATCTGCC AGGCCCTGAC GGTGGCGGGC 
AGCATCCTGG CCAAAGCACA GCTTGAGGTC ACAGACGTGT TGACCGATCG CCCTCCACCC 
ATCATCCGGC AGGGTCCAGC TAACCAGACT CTTGGTGTAG ACAGTGTGGC TCTTTTGAGG 
TGCCATGCAT CCGGAGAACC AGTCCCAACC ATCAGCTGGC TGAAGGACGG GGTCAGTCTG 
CTGGGCAAAG ACCCTCGGAT GTCCCTGCAA GACCTCGGCA GCCTGCAGAT CAAGGGTTTA 
CGGCTCTCTG ATTCTGGCAT TTATACTTGT GTGGCTGCAA GTTCTAGTGG GGAAACATCA 
TGGAGTGCTT TCTTGGAGGT CAAAGAGGCT GGGAGTCTTG TTGTAGTTAA AGATCGGGAT 
GAGAACGAGC TTCCAGGACC TCCTTCCAAA CCTCAGGTCA CAGATGTCAC TAAAAACAGC 
GTGTCATTAT CCTGGCAGCC AGGCCTTCCC GGAGCATCAG CCATTTCTAC ATATGTCATA 
GAAGCATTCA GCCAATCGGT GAGCAACAGC TGGCAAACTG TTGCCGATCA CGCCAAGACA 
ACGCAGTACA CCATTAAGGG TCTCCGCCCT AACACCATCT ACCTGTTCAT GGTGCGAGCG 
GTCAACGTCC AAGGCCTGAG CGACCCAAGC CCCATGTCTG AACCCGTCCG CACTCAAGAT 
ATAAGTCCTC CAGCGCAGGG TGTGGATCAC AGACATGTGC AAAAGGAGCT GGGAGAAGTT 
ATTGTGCGGC TACACAACCC TGTGGTCCTT AGCCCTACCA CCATACAGGT CACATGGACG 
GTTGACCGCC AGTCTCAGTT CATCCAGGGC TACAGGGTTT TGTACAGGCA GATGTCAGGC 
CTGTCTTCTC CAGGAGCCTG GCAAACTCAG GATGTGAAAG TCCCCTCAGA GAGGAGCATG 
GTGCTCTCGG CTCTAAAGAA AGGCATCGTC TACGAGATCA AAGTGCGTCC ATATTTCAAT 
GAGTTTCAGG GCATGGACAG CGAATCCCGA ACCGCAAGAA CAACAGAGGA AGCGCCCAGT 
GCTCCTCCTC AGCAGGTGAC TGTACTCACT GTGGGCAACC AGAACAGCAC ATCTATTAGC 
ATCTCCTGGG ACCCTCCGCC TGCAGAACAC CAGAACGGCA TCATACAGGA GTACAAGATC 
TGGTGTTTGG GGAATGAGAC CCGTTTTCAT GTGAATAAGA CGGTGGACGC AGCGATCCGC 
TCAGTGGTGG TTGGGGGTCT CCAGGCGGGG GTCCAGTATC GGGTGGAGGT GGCCGCTAGC 
ACCAGCGCCG GGGTCGGAGT GAAGAGCGAA CCACAAACCA TCATCATTGG TAAAGACTTC 
CGCGATGTGA TGATTAGCAG GAATAACAGC ATCACAGAGC AGATCACGGA TGTGGTGAAA 
CAGCCGGCCT TCATCGCGGG CATTGGCGGG GCCTGCTGGG TCATTCTGAT GGGCTTCAGC 
ATCTGGCTCT ACTGGAGGAG GAAGAAGAGA AAGGGACTCA GCAATTACGC AGTTCAGTCG 
TTCACCTTCA CCCCTGCAGT GACGTTCCAG CGTGTTGATG GAGGCTTGAT GAGCAACGGG 
AGTCGTCCTG GTCTGCTGAA CGCCAGTGAT TCGGGTTACC CGTGGCTCGC CGACTCCTGG 
CCTCCCAGCC TGCCTGTCAA CAGCGGATCT GGAGGACCCA ATGACCTCAG CTTTGGCCGT 
GGAGATGTGA TGCCCAGTGC TCAGGCTGAT AAGACGGGTA CCATGCTTTC GGATGGAGCC 
ATTTACAGCA GCATCGACTT CACCACCAAA GGCGGATTCG GTAGCCCAAG CCCAGCCTCC 
CAGGCCACGC CCTATGCCAC CACACAGATC CTCCACTCCA ACAGCATCCA TGAGCTGGCA 
GTGGATCTTC CTGAAGCCCA GTGGAAGACC TCCCTACAGG CCAAGCAGGA GATGGCCAGC 
CTGGGTTACT CACTGCCCGA CAAAAACTCC TGCAATAACA CACTCCTTTT CATTCCTGAC 
TACCGATTGG CTGACGGACT GTCTAATAGA ATGCCACACA ACCAATCGCA GGATTTCAGC 
ACCACCAGCT CCCACAACAG CTCAGATCGG AGCAACAGTC TGTCAGGTGG AAAAGGTGGT 
AAGAAGAAGA AGACCAAAGC AAACGTCAAG CCCACCAAGA TGAATGGCTC CAACTGGGCG 
AACGTTCCAC TTCCTCCTCC TCCCATCCAC CCTCTTCCTG GCACAGAGAT GGACCACTAC 
GCCTCAGAGC ACCATGATGG AGGAGGATAT GATAGTGATG GTTGGGGCCC ACCATTGCCA 
GTGCAGACGT ACCTCCATCA GGGTTTAGAG GATGAGCTGG AGGAAGAGGA AGAGCGGGTG 
CCAACTCCTC CCATTAGGGG TGTGGCTTCT TCTCCTGCTG CAGTTTCCTT TGGCAAACAG 
TCCACAGCCA CACTGAGCCC ATCTCCACGT GAAGAAATGC AGCCCATGCT CCAAGCCCAC 
CTAGATGAGC TTACCCGTGC TTATCAATTG GACATGGCTA AACAGACTTG GCACATGCAG 
GGTGGGCCTT ACCCGCCCCA GGCCCCCGCA CCACCCGTGG GCTACGTCTC CAGCACCTTA 
GTGTCTGACA TCGAGACCGA TCTCCCTGAT GAAGATGACG ATGATGAGGA GGATGAAGGA 
TACGAGATGT CGCCACCAAT TAGGGGCACT GAACACACGC CAGGCTCCAG CATGGACAAC 
TTAGACAGCT CCGTTACGGG CTCTATGGTG AACGGCTGGG GCTCGGCTTC AGAAGATGAT 
GACAGAAACT TCTCCAGCAG GAGGTCTAGT GCCGGCAGCT CTTCTGACGG CTCGCTCTTC 
ACCCATTGCA GCTTTGCGCG AGCCCTGGTG GCAGCCGCAG ACAAGGCTGG TTACCGTCTG 
GATGGCACCA GCCTGAGCAA AAGAGATAAA GGTCTACCTC ATAGACCTCG TCCCGCTAGC 
CCGTTCTCCA CAGATGGAAG CACGGTGGGC ACACACAGTC TGGGGCACCA GCGGGCAGCA 
AGGCCCACAC GTAAACACAA AGGCCCTGGC ACTGTCCCGA ACCGCCGTGA TGCCAGTGCG 
GATGATCTGC CTCCACCTCC AGAGCCTCCA CCCTGCCAGA CAGCTCGCAT CCCCGGGTCG 
AGGAACGTTC CCCATCCCTG CGACCGCAAA GCTTCCTCTC TGGAGAGGCA GCCTATGATG 
GGCCTTGAGG AAATGAGAAG TTCCTTGGAC CGGCAGGCCC GAACCTCATT GGACCGCCAC 
CAACAAGTCC AGGACAGACT GGGGTCCATA GAGAGAGGAG ATGAAAGCAG AAGAGGACAG 
AAAAATGGTC CCTTTTCAGA AGAGGCCCTA TTGCCATACA ACAAACCATC ATTCCCATCC 
CCTGGTGGGC ACAGCTCATC TGGGACGGCC TCTTCTAAAG GGTCAACGGG TCCTCGCAAA 
GGAGAAGGGA CGAGGGGACA GCACCAGCGC TCCACCACGG AACAAGGAGA AAGCAGCTAC 
ATGGGGACCA ACGCGCAGTT TTCCGGAGAG TTATGTGAGT CTGAACAAGT TCAAGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017206487.1
CDS	680..5479
Translation	MFLLSPVRLTLFLLVFLDVPHPSLQGRNKGRWNKGGSRLRQEDSPPRIVEHPSDLIVSKGEPATLNCKAEGRPTPTVEWYKDGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRRSKPDEGAYVCVARNYLGEAVSRNASLEVALLRDDFRQNPTDVVVAAGEPAILECVPPRGHPEPTIYWKKDKVRIDEKDDRIKIRGGKLMISNTRKSDAGMYICVGTNMVGERDSETAQVTVFERPTFLRRPINQVVLEEEAVEFRCQVQGDPQPSIRWKKDDVEIPRGRYDMKFDKDDFTLRIKKATASDEGTFTCVAENRVGKMEASATLTVRARPVAAPQFVIRPRDQIVAQGRTASFPCETKGNPQPAVFWQKEGSQNLLFPNQPQQPNSRFSVSPSGDLTITTVQRADAGYYICQALTVAGSILAKAQLEVTDVLTDRPPPIIRQGPANQTLGVDSVALLRCHASGEPVPTISWLKDGVSLLGKDPRMSLQDLGSLQIKGLRLSDSGIYTCVAASSSGETSWSAFLEVKEAGSLVVVKDRDENELPGPPSKPQVTDVTKNSVSLSWQPGLPGASAISTYVIEAFSQSVSNSWQTVADHAKTTQYTIKGLRPNTIYLFMVRAVNVQGLSDPSPMSEPVRTQDISPPAQGVDHRHVQKELGEVIVRLHNPVVLSPTTIQVTWTVDRQSQFIQGYRVLYRQMSGLSSPGAWQTQDVKVPSERSMVLSALKKGIVYEIKVRPYFNEFQGMDSESRTARTTEEAPSAPPQQVTVLTVGNQNSTSISISWDPPPAEHQNGIIQEYKIWCLGNETRFHVNKTVDAAIRSVVVGGLQAGVQYRVEVAASTSAGVGVKSEPQTIIIGKDFRDVMISRNNSITEQITDVVKQPAFIAGIGGACWVILMGFSIWLYWRRKKRKGLSNYAVQSFTFTPAVTFQRVDGGLMSNGSRPGLLNASDSGYPWLADSWPPSLPVNSGSGGPNDLSFGRGDVMPSAQADKTGTMLSDGAIYSSIDFTTKGGFGSPSPASQATPYATTQILHSNSIHELAVDLPEAQWKTSLQAKQEMASLGYSLPDKNSCNNTLLFIPDYRLADGLSNRMPHNQSQDFSTTSSHNSSDRSNSLSGGKGGKKKKTKANVKPTKMNGSNWANVPLPPPPIHPLPGTEMDHYASEHHDGGGYDSDGWGPPLPVQTYLHQGLEDELEEEEERVPTPPIRGVASSPAAVSFGKQSTATLSPSPREEMQPMLQAHLDELTRAYQLDMAKQTWHMQGGPYPPQAPAPPVGYVSSTLVSDIETDLPDEDDDDEEDEGYEMSPPIRGTEHTPGSSMDNLDSSVTGSMVNGWGSASEDDDRNFSSRRSSAGSSSDGSLFTHCSFARALVAAADKAGYRLDGTSLSKRDKGLPHRPRPASPFSTDGSTVGTHSLGHQRAARPTRKHKGPGTVPNRRDASADDLPPPPEPPPCQTARIPGSRNVPHPCDRKASSLERQPMMGLEEMRSSLDRQARTSLDRHQQVQDRLGSIERGDESRRGQKNGPFSEEALLPYNKPSFPSPGGHSSSGTASSKGSTGPRKGEGTRGQHQRSTTEQGESSYMGTNAQFSGELCESEQVQE

Target ORF information:

RefSeq Version	XM_017350998.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio roundabout, axon guidance receptor, homolog 2 (Drosophila) (robo2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017350998.1

ORF Insert Sequence:

ATGTTCCTCC TCTCTCCTGT GAGACTCACT TTATTTCTGT TGGTCTTCCT GGATGTTCCT 
CATCCGTCCC TTCAGGGCAG GAATAAGGGC AGATGGAACA AGGGGGGATC CCGTCTGCGG 
CAGGAAGATT CTCCACCTCG GATTGTAGAA CACCCCTCCG ATCTGATCGT GTCAAAGGGC 
GAGCCAGCCA CCCTAAACTG CAAGGCGGAG GGTCGGCCAA CTCCCACCGT GGAGTGGTAC 
AAGGATGGAG AACGAGTTGA AACGGACAAA GATGACCCTC GATCTCACCG CATGCTCCTT 
CCCAGTGGTT CGCTCTTCTT CCTGCGTATC GTACATGGAC GCCGCAGCAA ACCTGATGAG 
GGGGCGTACG TCTGTGTGGC ACGCAACTAC TTGGGGGAAG CAGTCAGTCG AAACGCCTCG 
CTGGAAGTAG CATTGTTACG GGATGATTTC CGGCAGAACC CTACAGATGT GGTGGTCGCT 
GCTGGAGAGC CGGCCATCCT GGAGTGTGTC CCTCCCAGAG GACACCCGGA GCCCACCATC 
TACTGGAAGA AAGACAAAGT CCGAATTGAT GAGAAGGATG ACAGGATCAA GATCCGTGGA 
GGGAAGCTGA TGATCTCTAA CACCAGGAAA AGCGACGCTG GAATGTACAT CTGCGTGGGC 
ACCAACATGG TCGGCGAAAG AGACAGTGAA ACAGCCCAGG TCACTGTGTT CGAGAGGCCC 
ACGTTCCTGC GGCGTCCCAT TAACCAGGTG GTTCTGGAGG AGGAGGCTGT GGAATTTCGA 
TGTCAGGTGC AGGGAGACCC GCAGCCGTCC ATCCGCTGGA AAAAAGACGA CGTTGAAATC 
CCCCGCGGCA GGTACGATAT GAAATTTGAT AAAGATGACT TCACGCTGAG AATAAAGAAG 
GCAACAGCGA GTGATGAAGG AACCTTCACC TGTGTAGCGG AGAACCGAGT GGGCAAAATG 
GAGGCCTCCG CAACCCTCAC GGTCAGAGCT CGCCCTGTCG CTGCACCGCA GTTCGTGATC 
AGGCCGCGGG ATCAGATTGT GGCACAGGGA CGCACCGCCA GCTTCCCCTG CGAGACTAAA 
GGGAACCCGC AGCCGGCAGT CTTCTGGCAG AAAGAAGGAA GTCAGAATCT CCTGTTTCCA 
AATCAACCCC AGCAGCCCAA CAGTCGTTTC TCGGTGTCGC CCAGTGGAGA TCTAACCATC 
ACTACGGTCC AGCGGGCAGA TGCGGGCTAT TATATCTGCC AGGCCCTGAC GGTGGCGGGC 
AGCATCCTGG CCAAAGCACA GCTTGAGGTC ACAGACGTGT TGACCGATCG CCCTCCACCC 
ATCATCCGGC AGGGTCCAGC TAACCAGACT CTTGGTGTAG ACAGTGTGGC TCTTTTGAGG 
TGCCATGCAT CCGGAGAACC AGTCCCAACC ATCAGCTGGC TGAAGGACGG GGTCAGTCTG 
CTGGGCAAAG ACCCTCGGAT GTCCCTGCAA GACCTCGGCA GCCTGCAGAT CAAGGGTTTA 
CGGCTCTCTG ATTCTGGCAT TTATACTTGT GTGGCTGCAA GTTCTAGTGG GGAAACATCA 
TGGAGTGCTT TCTTGGAGGT CAAAGAGGCT GGGAGTCTTG TTGTAGTTAA AGATCGGGAT 
GAGAACGAGC TTCCAGGACC TCCTTCCAAA CCTCAGGTCA CAGATGTCAC TAAAAACAGC 
GTGTCATTAT CCTGGCAGCC AGGCCTTCCC GGAGCATCAG CCATTTCTAC ATATGTCATA 
GAAGCATTCA GCCAATCGGT GAGCAACAGC TGGCAAACTG TTGCCGATCA CGCCAAGACA 
ACGCAGTACA CCATTAAGGG TCTCCGCCCT AACACCATCT ACCTGTTCAT GGTGCGAGCG 
GTCAACGTCC AAGGCCTGAG CGACCCAAGC CCCATGTCTG AACCCGTCCG CACTCAAGAT 
ATAAGTCCTC CAGCGCAGGG TGTGGATCAC AGACATGTGC AAAAGGAGCT GGGAGAAGTT 
ATTGTGCGGC TACACAACCC TGTGGTCCTT AGCCCTACCA CCATACAGGT CACATGGACG 
GTTGACCGCC AGTCTCAGTT CATCCAGGGC TACAGGGTTT TGTACAGGCA GATGTCAGGC 
CTGTCTTCTC CAGGAGCCTG GCAAACTCAG GATGTGAAAG TCCCCTCAGA GAGGAGCATG 
GTGCTCTCGG CTCTAAAGAA AGGCATCGTC TACGAGATCA AAGTGCGTCC ATATTTCAAT 
GAGTTTCAGG GCATGGACAG CGAATCCCGA ACCGCAAGAA CAACAGAGGA AGCGCCCAGT 
GCTCCTCCTC AGCAGGTGAC TGTACTCACT GTGGGCAACC AGAACAGCAC ATCTATTAGC 
ATCTCCTGGG ACCCTCCGCC TGCAGAACAC CAGAACGGCA TCATACAGGA GTACAAGATC 
TGGTGTTTGG GGAATGAGAC CCGTTTTCAT GTGAATAAGA CGGTGGACGC AGCGATCCGC 
TCAGTGGTGG TTGGGGGTCT CCAGGCGGGG GTCCAGTATC GGGTGGAGGT GGCCGCTAGC 
ACCAGCGCCG GGGTCGGAGT GAAGAGCGAA CCACAAACCA TCATCATTGG TAAAGACTTC 
CGCGATGTGA TGATTAGCAG GAATAACAGC ATCACAGAGC AGATCACGGA TGTGGTGAAA 
CAGCCGGCCT TCATCGCGGG CATTGGCGGG GCCTGCTGGG TCATTCTGAT GGGCTTCAGC 
ATCTGGCTCT ACTGGAGGAG GAAGAAGAGA AAGGGACTCA GCAATTACGC AGTTCAGTCG 
TTCACCTTCA CCCCTGCAGT GACGTTCCAG CGTGTTGATG GAGGCTTGAT GAGCAACGGG 
AGTCGTCCTG GTCTGCTGAA CGCCAGTGAT TCGGGTTACC CGTGGCTCGC CGACTCCTGG 
CCTCCCAGCC TGCCTGTCAA CAGCGGATCT GGAGGACCCA ATGACCTCAG CTTTGGCCGT 
GGAGATGTGA TGCCCAGTGC TCAGGCTGAT AAGACGGGTA CCATGCTTTC GGATGGAGCC 
ATTTACAGCA GCATCGACTT CACCACCAAA GGCGGATTCG GTAGCCCAAG CCCAGCCTCC 
CAGGCCACGC CCTATGCCAC CACACAGATC CTCCACTCCA ACAGCATCCA TGAGCTGGCA 
GTGGATCTTC CTGAAGCCCA GTGGAAGACC TCCCTACAGG CCAAGCAGGA GATGGCCAGC 
CTGGGTTACT CACTGCCCGA CAAAAACTCC TGCAATAACA CACTCCTTTT CATTCCTGAC 
TACCGATTGG CTGACGGACT GTCTAATAGA ATGCCACACA ACCAATCGCA GGATTTCAGC 
ACCACCAGCT CCCACAACAG CTCAGATCGG AGCAACAGTC TGTCAGGTGG AAAAGGTGGT 
AAGAAGAAGA AGACCAAAGC AAACGTCAAG CCCACCAAGA TGAATGGCTC CAACTGGGCG 
AACGTTCCAC TTCCTCCTCC TCCCATCCAC CCTCTTCCTG GCACAGAGAT GGACCACTAC 
GCCTCAGAGC ACCATGATGG AGGAGGATAT GATAGTGATG GTTGGGGCCC ACCATTGCCA 
GTGCAGACGT ACCTCCATCA GGGTTTAGAG GATGAGCTGG AGGAAGAGGA AGAGCGGGTG 
CCAACTCCTC CCATTAGGGG TGTGGCTTCT TCTCCTGCTG CAGTTTCCTT TGGCAAACAG 
TCCACAGCCA CACTGAGCCC ATCTCCACGT GAAGAAATGC AGCCCATGCT CCAAGCCCAC 
CTAGATGAGC TTACCCGTGC TTATCAATTG GACATGGCTA AACAGACTTG GCACATGCAG 
GGTGGGCCTT ACCCGCCCCA GGCCCCCGCA CCACCCGTGG GCTACGTCTC CAGCACCTTA 
GTGTCTGACA TCGAGACCGA TCTCCCTGAT GAAGATGACG ATGATGAGGA GGATGAAGGA 
TACGAGATGT CGCCACCAAT TAGGGGCACT GAACACACGC CAGGCTCCAG CATGGACAAC 
TTAGACAGCT CCGTTACGGG CTCTATGGTG AACGGCTGGG GCTCGGCTTC AGAAGATGAT 
GACAGAAACT TCTCCAGCAG GAGGTCTAGT GCCGGCAGCT CTTCTGACGG CTCGCTCTTC 
ACCCATTGCA GCTTTGCGCG AGCCCTGGTG GCAGCCGCAG ACAAGGCTGG TTACCGTCTG 
GATGGCACCA GCCTGAGCAA AAGAGATAAA GGTCTACCTC ATAGACCTCG TCCCGCTAGC 
CCGTTCTCCA CAGATGGAAG CACGGTGGGC ACACACAGTC TGGGGCACCA GCGGGCAGCA 
AGGCCCACAC GTAAACACAA AGGCCCTGGC ACTGTCCCGA ACCGCCGTGA TGCCAGTGCG 
GATGATCTGC CTCCACCTCC AGAGCCTCCA CCCTGCCAGA CAGCTCGCAT CCCCGGGTCG 
AGGAACGTTC CCCATCCCTG CGACCGCAAA GCTTCCTCTC TGGAGAGGCA GCCTATGATG 
GGCCTTGAGG AAATGAGAAG TTCCTTGGAC CGGCAGGCCC GAACCTCATT GGACCGCCAC 
CAACAAGTCC AGGACAGACT GGGGTCCATA GAGAGAGGAG ATGAAAGCAG AAGAGGACAG 
AAAAATGGTC CCTTTTCAGA AGAGGCCCTA TTGCCATACA ACAAACCATC ATTCCCATCC 
CCTGGTGGGC ACAGCTCATC TGGGACGGCC TCTTCTAAAG GGTCAACGGG TCCTCGCAAA 
GGAGAAGGGA CGAGGGGACA GCACCAGCGC TCCACCACGG AACAAGGAGA AAGCAGCTAC 
ATGGGGACCA ACGCGCAGTT TTCCGGAGAG TTATGTGAGT CTGAACAAGT TCAAGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

robo2 ( XM_017350999.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017206488.1 Danio rerio roundabout, axon guidance receptor, homolog 2 (Drosophila) (robo2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa55118
Clone ID Related Accession (Same CDS sequence)	XM_017350999.1
Accession Version	XM_017350999.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4788bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	roundabout homolog 2 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCCTCC TCTCTCCTGT GAGACTCACT TTATTTCTGT TGGTCTTCCT GGATGTTCCT 
CATCCGTCCC TTCAGGGCAG GAATAAGGGC AGATGGAACA AGGGGGGATC CCGTCTGCGG 
CAGGAAGATT CTCCACCTCG GATTGTAGAA CACCCCTCCG ATCTGATCGT GTCAAAGGGC 
GAGCCAGCCA CCCTAAACTG CAAGGCGGAG GGTCGGCCAA CTCCCACCGT GGAGTGGTAC 
AAGGATGGAG AACGAGTTGA AACGGACAAA GATGACCCTC GATCTCACCG CATGCTCCTT 
CCCAGTGGTT CGCTCTTCTT CCTGCGTATC GTACATGGAC GCCGCAGCAA ACCTGATGAG 
GGGGCGTACG TCTGTGTGGC ACGCAACTAC TTGGGGGAAG CAGTCAGTCG AAACGCCTCG 
CTGGAAGTAG CATTGTTACG GGATGATTTC CGGCAGAACC CTACAGATGT GGTGGTCGCT 
GCTGGAGAGC CGGCCATCCT GGAGTGTGTC CCTCCCAGAG GACACCCGGA GCCCACCATC 
TACTGGAAGA AAGACAAAGT CCGAATTGAT GAGAAGGATG ACAGGATCAA GATCCGTGGA 
GGGAAGCTGA TGATCTCTAA CACCAGGAAA AGCGACGCTG GAATGTACAT CTGCGTGGGC 
ACCAACATGG TCGGCGAAAG AGACAGTGAA ACAGCCCAGG TCACTGTGTT CGAGAGGCCC 
ACGTTCCTGC GGCGTCCCAT TAACCAGGTG GTTCTGGAGG AGGAGGCTGT GGAATTTCGA 
TGTCAGGTGC AGGGAGACCC GCAGCCGTCC ATCCGCTGGA AAAAAGACGA CGTTGAAATC 
CCCCGCGGCA GGTACGATAT GAAATTTGAT AAAGATGACT TCACGCTGAG AATAAAGAAG 
GCAACAGCGA GTGATGAAGG AACCTTCACC TGTGTAGCGG AGAACCGAGT GGGCAAAATG 
GAGGCCTCCG CAACCCTCAC GGTCAGAGCT GCACCGCAGT TCGTGATCAG GCCGCGGGAT 
CAGATTGTGG CACAGGGACG CACCGCCAGC TTCCCCTGCG AGACTAAAGG GAACCCGCAG 
CCGGCAGTCT TCTGGCAGAA AGAAGGAAGT CAGAATCTCC TGTTTCCAAA TCAACCCCAG 
CAGCCCAACA GTCGTTTCTC GGTGTCGCCC AGTGGAGATC TAACCATCAC TACGGTCCAG 
CGGGCAGATG CGGGCTATTA TATCTGCCAG GCCCTGACGG TGGCGGGCAG CATCCTGGCC 
AAAGCACAGC TTGAGGTCAC AGACGTGTTG ACCGATCGCC CTCCACCCAT CATCCGGCAG 
GGTCCAGCTA ACCAGACTCT TGGTGTAGAC AGTGTGGCTC TTTTGAGGTG CCATGCATCC 
GGAGAACCAG TCCCAACCAT CAGCTGGCTG AAGGACGGGG TCAGTCTGCT GGGCAAAGAC 
CCTCGGATGT CCCTGCAAGA CCTCGGCAGC CTGCAGATCA AGGGTTTACG GCTCTCTGAT 
TCTGGCATTT ATACTTGTGT GGCTGCAAGT TCTAGTGGGG AAACATCATG GAGTGCTTTC 
TTGGAGGTCA AAGAGGCTGG GAGTCTTGTT GTAGTTAAAG ATCGGGATGA GAACGAGCTT 
CCAGGACCTC CTTCCAAACC TCAGGTCACA GATGTCACTA AAAACAGCGT GTCATTATCC 
TGGCAGCCAG GCCTTCCCGG AGCATCAGCC ATTTCTACAT ATGTCATAGA AGCATTCAGC 
CAATCGGTGA GCAACAGCTG GCAAACTGTT GCCGATCACG CCAAGACAAC GCAGTACACC 
ATTAAGGGTC TCCGCCCTAA CACCATCTAC CTGTTCATGG TGCGAGCGGT CAACGTCCAA 
GGCCTGAGCG ACCCAAGCCC CATGTCTGAA CCCGTCCGCA CTCAAGATAT AAGTCCTCCA 
GCGCAGGGTG TGGATCACAG ACATGTGCAA AAGGAGCTGG GAGAAGTTAT TGTGCGGCTA 
CACAACCCTG TGGTCCTTAG CCCTACCACC ATACAGGTCA CATGGACGGT TGACCGCCAG 
TCTCAGTTCA TCCAGGGCTA CAGGGTTTTG TACAGGCAGA TGTCAGGCCT GTCTTCTCCA 
GGAGCCTGGC AAACTCAGGA TGTGAAAGTC CCCTCAGAGA GGAGCATGGT GCTCTCGGCT 
CTAAAGAAAG GCATCGTCTA CGAGATCAAA GTGCGTCCAT ATTTCAATGA GTTTCAGGGC 
ATGGACAGCG AATCCCGAAC CGCAAGAACA ACAGAGGAAG CGCCCAGTGC TCCTCCTCAG 
CAGGTGACTG TACTCACTGT GGGCAACCAG AACAGCACAT CTATTAGCAT CTCCTGGGAC 
CCTCCGCCTG CAGAACACCA GAACGGCATC ATACAGGAGT ACAAGATCTG GTGTTTGGGG 
AATGAGACCC GTTTTCATGT GAATAAGACG GTGGACGCAG CGATCCGCTC AGTGGTGGTT 
GGGGGTCTCC AGGCGGGGGT CCAGTATCGG GTGGAGGTGG CCGCTAGCAC CAGCGCCGGG 
GTCGGAGTGA AGAGCGAACC ACAAACCATC ATCATTGGTA AAGACTTCCG CGATGTGATG 
ATTAGCAGGA ATAACAGCAT CACAGAGCAG ATCACGGATG TGGTGAAACA GCCGGCCTTC 
ATCGCGGGCA TTGGCGGGGC CTGCTGGGTC ATTCTGATGG GCTTCAGCAT CTGGCTCTAC 
TGGAGGAGGA AGAAGAGAAA GGGACTCAGC AATTACGCAG TTCAGTCGTT CACCTTCACC 
CCTGCAGTGA CGTTCCAGCG TGTTGATGGA GGCTTGATGA GCAACGGGAG TCGTCCTGGT 
CTGCTGAACG CCAGTGATTC GGGTTACCCG TGGCTCGCCG ACTCCTGGCC TCCCAGCCTG 
CCTGTCAACA GCGGATCTGG AGGACCCAAT GACCTCAGCT TTGGCCGTGG AGATGTGATG 
CCCAGTGCTC AGGCTGATAA GACGGGTACC ATGCTTTCGG ATGGAGCCAT TTACAGCAGC 
ATCGACTTCA CCACCAAAGG CGGATTCGGT AGCCCAAGCC CAGCCTCCCA GGCCACGCCC 
TATGCCACCA CACAGATCCT CCACTCCAAC AGCATCCATG AGCTGGCAGT GGATCTTCCT 
GAAGCCCAGT GGAAGACCTC CCTACAGGCC AAGCAGGAGA TGGCCAGCCT GGGTTACTCA 
CTGCCCGACA AAAACTCCTG CAATAACACA CTCCTTTTCA TTCCTGACTA CCGATTGGCT 
GACGGACTGT CTAATAGAAT GCCACACAAC CAATCGCAGG ATTTCAGCAC CACCAGCTCC 
CACAACAGCT CAGATCGGAG CAACAGTCTG TCAGGTGGAA AAGGTGGTAA GAAGAAGAAG 
ACCAAAGCAA ACGTCAAGCC CACCAAGATG AATGGCTCCA ACTGGGCGAA CGTTCCACTT 
CCTCCTCCTC CCATCCACCC TCTTCCTGGC ACAGAGATGG ACCACTACGC CTCAGAGCAC 
CATGATGGAG GAGGATATGA TAGTGATGGT TGGGGCCCAC CATTGCCAGT GCAGACGTAC 
CTCCATCAGG GTTTAGAGGA TGAGCTGGAG GAAGAGGAAG AGCGGGTGCC AACTCCTCCC 
ATTAGGGGTG TGGCTTCTTC TCCTGCTGCA GTTTCCTTTG GCAAACAGTC CACAGCCACA 
CTGAGCCCAT CTCCACGTGA AGAAATGCAG CCCATGCTCC AAGCCCACCT AGATGAGCTT 
ACCCGTGCTT ATCAATTGGA CATGGCTAAA CAGACTTGGC ACATGCAGGG TGGGCCTTAC 
CCGCCCCAGG CCCCCGCACC ACCCGTGGGC TACGTCTCCA GCACCTTAGT GTCTGACATC 
GAGACCGATC TCCCTGATGA AGATGACGAT GATGAGGAGG ATGAAGGATA CGAGATGTCG 
CCACCAATTA GGGGCACTGA ACACACGCCA GGCTCCAGCA TGGACAACTT AGACAGCTCC 
GTTACGGGCT CTATGGTGAA CGGCTGGGGC TCGGCTTCAG AAGATGATGA CAGAAACTTC 
TCCAGCAGGA GGTCTAGTGC CGGCAGCTCT TCTGACGGCT CGCTCTTCAC CCATTGCAGC 
TTTGCGCGAG CCCTGGTGGC AGCCGCAGAC AAGGCTGGTT ACCGTCTGGA TGGCACCAGC 
CTGAGCAAAA GAGATAAAGG TCTACCTCAT AGACCTCGTC CCGCTAGCCC GTTCTCCACA 
GATGGAAGCA CGGTGGGCAC ACACAGTCTG GGGCACCAGC GGGCAGCAAG GCCCACACGT 
AAACACAAAG GCCCTGGCAC TGTCCCGAAC CGCCGTGATG CCAGTGCGGA TGATCTGCCT 
CCACCTCCAG AGCCTCCACC CTGCCAGACA GCTCGCATCC CCGGGTCGAG GAACGTTCCC 
CATCCCTGCG ACCGCAAAGC TTCCTCTCTG GAGAGGCAGC CTATGATGGG CCTTGAGGAA 
ATGAGAAGTT CCTTGGACCG GCAGGCCCGA ACCTCATTGG ACCGCCACCA ACAAGTCCAG 
GACAGACTGG GGTCCATAGA GAGAGGAGAT GAAAGCAGAA GAGGACAGAA AAATGGTCCC 
TTTTCAGAAG AGGCCCTATT GCCATACAAC AAACCATCAT TCCCATCCCC TGGTGGGCAC 
AGCTCATCTG GGACGGCCTC TTCTAAAGGG TCAACGGGTC CTCGCAAAGG AGAAGGGACG 
AGGGGACAGC ACCAGCGCTC CACCACGGAA CAAGGAGAAA GCAGCTACAT GGGGACCAAC 
GCGCAGTTTT CCGGAGAGTT ATGTGAGTCT GAACAAGTTC AAGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017206488.1
CDS	680..5467
Translation	MFLLSPVRLTLFLLVFLDVPHPSLQGRNKGRWNKGGSRLRQEDSPPRIVEHPSDLIVSKGEPATLNCKAEGRPTPTVEWYKDGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRRSKPDEGAYVCVARNYLGEAVSRNASLEVALLRDDFRQNPTDVVVAAGEPAILECVPPRGHPEPTIYWKKDKVRIDEKDDRIKIRGGKLMISNTRKSDAGMYICVGTNMVGERDSETAQVTVFERPTFLRRPINQVVLEEEAVEFRCQVQGDPQPSIRWKKDDVEIPRGRYDMKFDKDDFTLRIKKATASDEGTFTCVAENRVGKMEASATLTVRAAPQFVIRPRDQIVAQGRTASFPCETKGNPQPAVFWQKEGSQNLLFPNQPQQPNSRFSVSPSGDLTITTVQRADAGYYICQALTVAGSILAKAQLEVTDVLTDRPPPIIRQGPANQTLGVDSVALLRCHASGEPVPTISWLKDGVSLLGKDPRMSLQDLGSLQIKGLRLSDSGIYTCVAASSSGETSWSAFLEVKEAGSLVVVKDRDENELPGPPSKPQVTDVTKNSVSLSWQPGLPGASAISTYVIEAFSQSVSNSWQTVADHAKTTQYTIKGLRPNTIYLFMVRAVNVQGLSDPSPMSEPVRTQDISPPAQGVDHRHVQKELGEVIVRLHNPVVLSPTTIQVTWTVDRQSQFIQGYRVLYRQMSGLSSPGAWQTQDVKVPSERSMVLSALKKGIVYEIKVRPYFNEFQGMDSESRTARTTEEAPSAPPQQVTVLTVGNQNSTSISISWDPPPAEHQNGIIQEYKIWCLGNETRFHVNKTVDAAIRSVVVGGLQAGVQYRVEVAASTSAGVGVKSEPQTIIIGKDFRDVMISRNNSITEQITDVVKQPAFIAGIGGACWVILMGFSIWLYWRRKKRKGLSNYAVQSFTFTPAVTFQRVDGGLMSNGSRPGLLNASDSGYPWLADSWPPSLPVNSGSGGPNDLSFGRGDVMPSAQADKTGTMLSDGAIYSSIDFTTKGGFGSPSPASQATPYATTQILHSNSIHELAVDLPEAQWKTSLQAKQEMASLGYSLPDKNSCNNTLLFIPDYRLADGLSNRMPHNQSQDFSTTSSHNSSDRSNSLSGGKGGKKKKTKANVKPTKMNGSNWANVPLPPPPIHPLPGTEMDHYASEHHDGGGYDSDGWGPPLPVQTYLHQGLEDELEEEEERVPTPPIRGVASSPAAVSFGKQSTATLSPSPREEMQPMLQAHLDELTRAYQLDMAKQTWHMQGGPYPPQAPAPPVGYVSSTLVSDIETDLPDEDDDDEEDEGYEMSPPIRGTEHTPGSSMDNLDSSVTGSMVNGWGSASEDDDRNFSSRRSSAGSSSDGSLFTHCSFARALVAAADKAGYRLDGTSLSKRDKGLPHRPRPASPFSTDGSTVGTHSLGHQRAARPTRKHKGPGTVPNRRDASADDLPPPPEPPPCQTARIPGSRNVPHPCDRKASSLERQPMMGLEEMRSSLDRQARTSLDRHQQVQDRLGSIERGDESRRGQKNGPFSEEALLPYNKPSFPSPGGHSSSGTASSKGSTGPRKGEGTRGQHQRSTTEQGESSYMGTNAQFSGELCESEQVQE

Target ORF information:

RefSeq Version	XM_017350999.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio roundabout, axon guidance receptor, homolog 2 (Drosophila) (robo2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017350999.1

ORF Insert Sequence:

ATGTTCCTCC TCTCTCCTGT GAGACTCACT TTATTTCTGT TGGTCTTCCT GGATGTTCCT 
CATCCGTCCC TTCAGGGCAG GAATAAGGGC AGATGGAACA AGGGGGGATC CCGTCTGCGG 
CAGGAAGATT CTCCACCTCG GATTGTAGAA CACCCCTCCG ATCTGATCGT GTCAAAGGGC 
GAGCCAGCCA CCCTAAACTG CAAGGCGGAG GGTCGGCCAA CTCCCACCGT GGAGTGGTAC 
AAGGATGGAG AACGAGTTGA AACGGACAAA GATGACCCTC GATCTCACCG CATGCTCCTT 
CCCAGTGGTT CGCTCTTCTT CCTGCGTATC GTACATGGAC GCCGCAGCAA ACCTGATGAG 
GGGGCGTACG TCTGTGTGGC ACGCAACTAC TTGGGGGAAG CAGTCAGTCG AAACGCCTCG 
CTGGAAGTAG CATTGTTACG GGATGATTTC CGGCAGAACC CTACAGATGT GGTGGTCGCT 
GCTGGAGAGC CGGCCATCCT GGAGTGTGTC CCTCCCAGAG GACACCCGGA GCCCACCATC 
TACTGGAAGA AAGACAAAGT CCGAATTGAT GAGAAGGATG ACAGGATCAA GATCCGTGGA 
GGGAAGCTGA TGATCTCTAA CACCAGGAAA AGCGACGCTG GAATGTACAT CTGCGTGGGC 
ACCAACATGG TCGGCGAAAG AGACAGTGAA ACAGCCCAGG TCACTGTGTT CGAGAGGCCC 
ACGTTCCTGC GGCGTCCCAT TAACCAGGTG GTTCTGGAGG AGGAGGCTGT GGAATTTCGA 
TGTCAGGTGC AGGGAGACCC GCAGCCGTCC ATCCGCTGGA AAAAAGACGA CGTTGAAATC 
CCCCGCGGCA GGTACGATAT GAAATTTGAT AAAGATGACT TCACGCTGAG AATAAAGAAG 
GCAACAGCGA GTGATGAAGG AACCTTCACC TGTGTAGCGG AGAACCGAGT GGGCAAAATG 
GAGGCCTCCG CAACCCTCAC GGTCAGAGCT GCACCGCAGT TCGTGATCAG GCCGCGGGAT 
CAGATTGTGG CACAGGGACG CACCGCCAGC TTCCCCTGCG AGACTAAAGG GAACCCGCAG 
CCGGCAGTCT TCTGGCAGAA AGAAGGAAGT CAGAATCTCC TGTTTCCAAA TCAACCCCAG 
CAGCCCAACA GTCGTTTCTC GGTGTCGCCC AGTGGAGATC TAACCATCAC TACGGTCCAG 
CGGGCAGATG CGGGCTATTA TATCTGCCAG GCCCTGACGG TGGCGGGCAG CATCCTGGCC 
AAAGCACAGC TTGAGGTCAC AGACGTGTTG ACCGATCGCC CTCCACCCAT CATCCGGCAG 
GGTCCAGCTA ACCAGACTCT TGGTGTAGAC AGTGTGGCTC TTTTGAGGTG CCATGCATCC 
GGAGAACCAG TCCCAACCAT CAGCTGGCTG AAGGACGGGG TCAGTCTGCT GGGCAAAGAC 
CCTCGGATGT CCCTGCAAGA CCTCGGCAGC CTGCAGATCA AGGGTTTACG GCTCTCTGAT 
TCTGGCATTT ATACTTGTGT GGCTGCAAGT TCTAGTGGGG AAACATCATG GAGTGCTTTC 
TTGGAGGTCA AAGAGGCTGG GAGTCTTGTT GTAGTTAAAG ATCGGGATGA GAACGAGCTT 
CCAGGACCTC CTTCCAAACC TCAGGTCACA GATGTCACTA AAAACAGCGT GTCATTATCC 
TGGCAGCCAG GCCTTCCCGG AGCATCAGCC ATTTCTACAT ATGTCATAGA AGCATTCAGC 
CAATCGGTGA GCAACAGCTG GCAAACTGTT GCCGATCACG CCAAGACAAC GCAGTACACC 
ATTAAGGGTC TCCGCCCTAA CACCATCTAC CTGTTCATGG TGCGAGCGGT CAACGTCCAA 
GGCCTGAGCG ACCCAAGCCC CATGTCTGAA CCCGTCCGCA CTCAAGATAT AAGTCCTCCA 
GCGCAGGGTG TGGATCACAG ACATGTGCAA AAGGAGCTGG GAGAAGTTAT TGTGCGGCTA 
CACAACCCTG TGGTCCTTAG CCCTACCACC ATACAGGTCA CATGGACGGT TGACCGCCAG 
TCTCAGTTCA TCCAGGGCTA CAGGGTTTTG TACAGGCAGA TGTCAGGCCT GTCTTCTCCA 
GGAGCCTGGC AAACTCAGGA TGTGAAAGTC CCCTCAGAGA GGAGCATGGT GCTCTCGGCT 
CTAAAGAAAG GCATCGTCTA CGAGATCAAA GTGCGTCCAT ATTTCAATGA GTTTCAGGGC 
ATGGACAGCG AATCCCGAAC CGCAAGAACA ACAGAGGAAG CGCCCAGTGC TCCTCCTCAG 
CAGGTGACTG TACTCACTGT GGGCAACCAG AACAGCACAT CTATTAGCAT CTCCTGGGAC 
CCTCCGCCTG CAGAACACCA GAACGGCATC ATACAGGAGT ACAAGATCTG GTGTTTGGGG 
AATGAGACCC GTTTTCATGT GAATAAGACG GTGGACGCAG CGATCCGCTC AGTGGTGGTT 
GGGGGTCTCC AGGCGGGGGT CCAGTATCGG GTGGAGGTGG CCGCTAGCAC CAGCGCCGGG 
GTCGGAGTGA AGAGCGAACC ACAAACCATC ATCATTGGTA AAGACTTCCG CGATGTGATG 
ATTAGCAGGA ATAACAGCAT CACAGAGCAG ATCACGGATG TGGTGAAACA GCCGGCCTTC 
ATCGCGGGCA TTGGCGGGGC CTGCTGGGTC ATTCTGATGG GCTTCAGCAT CTGGCTCTAC 
TGGAGGAGGA AGAAGAGAAA GGGACTCAGC AATTACGCAG TTCAGTCGTT CACCTTCACC 
CCTGCAGTGA CGTTCCAGCG TGTTGATGGA GGCTTGATGA GCAACGGGAG TCGTCCTGGT 
CTGCTGAACG CCAGTGATTC GGGTTACCCG TGGCTCGCCG ACTCCTGGCC TCCCAGCCTG 
CCTGTCAACA GCGGATCTGG AGGACCCAAT GACCTCAGCT TTGGCCGTGG AGATGTGATG 
CCCAGTGCTC AGGCTGATAA GACGGGTACC ATGCTTTCGG ATGGAGCCAT TTACAGCAGC 
ATCGACTTCA CCACCAAAGG CGGATTCGGT AGCCCAAGCC CAGCCTCCCA GGCCACGCCC 
TATGCCACCA CACAGATCCT CCACTCCAAC AGCATCCATG AGCTGGCAGT GGATCTTCCT 
GAAGCCCAGT GGAAGACCTC CCTACAGGCC AAGCAGGAGA TGGCCAGCCT GGGTTACTCA 
CTGCCCGACA AAAACTCCTG CAATAACACA CTCCTTTTCA TTCCTGACTA CCGATTGGCT 
GACGGACTGT CTAATAGAAT GCCACACAAC CAATCGCAGG ATTTCAGCAC CACCAGCTCC 
CACAACAGCT CAGATCGGAG CAACAGTCTG TCAGGTGGAA AAGGTGGTAA GAAGAAGAAG 
ACCAAAGCAA ACGTCAAGCC CACCAAGATG AATGGCTCCA ACTGGGCGAA CGTTCCACTT 
CCTCCTCCTC CCATCCACCC TCTTCCTGGC ACAGAGATGG ACCACTACGC CTCAGAGCAC 
CATGATGGAG GAGGATATGA TAGTGATGGT TGGGGCCCAC CATTGCCAGT GCAGACGTAC 
CTCCATCAGG GTTTAGAGGA TGAGCTGGAG GAAGAGGAAG AGCGGGTGCC AACTCCTCCC 
ATTAGGGGTG TGGCTTCTTC TCCTGCTGCA GTTTCCTTTG GCAAACAGTC CACAGCCACA 
CTGAGCCCAT CTCCACGTGA AGAAATGCAG CCCATGCTCC AAGCCCACCT AGATGAGCTT 
ACCCGTGCTT ATCAATTGGA CATGGCTAAA CAGACTTGGC ACATGCAGGG TGGGCCTTAC 
CCGCCCCAGG CCCCCGCACC ACCCGTGGGC TACGTCTCCA GCACCTTAGT GTCTGACATC 
GAGACCGATC TCCCTGATGA AGATGACGAT GATGAGGAGG ATGAAGGATA CGAGATGTCG 
CCACCAATTA GGGGCACTGA ACACACGCCA GGCTCCAGCA TGGACAACTT AGACAGCTCC 
GTTACGGGCT CTATGGTGAA CGGCTGGGGC TCGGCTTCAG AAGATGATGA CAGAAACTTC 
TCCAGCAGGA GGTCTAGTGC CGGCAGCTCT TCTGACGGCT CGCTCTTCAC CCATTGCAGC 
TTTGCGCGAG CCCTGGTGGC AGCCGCAGAC AAGGCTGGTT ACCGTCTGGA TGGCACCAGC 
CTGAGCAAAA GAGATAAAGG TCTACCTCAT AGACCTCGTC CCGCTAGCCC GTTCTCCACA 
GATGGAAGCA CGGTGGGCAC ACACAGTCTG GGGCACCAGC GGGCAGCAAG GCCCACACGT 
AAACACAAAG GCCCTGGCAC TGTCCCGAAC CGCCGTGATG CCAGTGCGGA TGATCTGCCT 
CCACCTCCAG AGCCTCCACC CTGCCAGACA GCTCGCATCC CCGGGTCGAG GAACGTTCCC 
CATCCCTGCG ACCGCAAAGC TTCCTCTCTG GAGAGGCAGC CTATGATGGG CCTTGAGGAA 
ATGAGAAGTT CCTTGGACCG GCAGGCCCGA ACCTCATTGG ACCGCCACCA ACAAGTCCAG 
GACAGACTGG GGTCCATAGA GAGAGGAGAT GAAAGCAGAA GAGGACAGAA AAATGGTCCC 
TTTTCAGAAG AGGCCCTATT GCCATACAAC AAACCATCAT TCCCATCCCC TGGTGGGCAC 
AGCTCATCTG GGACGGCCTC TTCTAAAGGG TCAACGGGTC CTCGCAAAGG AGAAGGGACG 
AGGGGACAGC ACCAGCGCTC CACCACGGAA CAAGGAGAAA GCAGCTACAT GGGGACCAAC 
GCGCAGTTTT CCGGAGAGTT ATGTGAGTCT GAACAAGTTC AAGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

robo2 ( XM_017351000.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017206489.1 Danio rerio roundabout, axon guidance receptor, homolog 2 (Drosophila) (robo2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa55119
Clone ID Related Accession (Same CDS sequence)	XM_017351000.1 , XM_017351000.2
Accession Version	XM_017351000.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4776bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	roundabout homolog 2 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCCTCC TCTCTCCTGT GAGACTCACT TTATTTCTGT TGGTCTTCCT GGATGTTCCT 
CATCCGTCCC TTCAGGGCAG GAATAAGGGC AGATGGAACA AGGGGGGATC CCGTCTGCGG 
CAGGAAGATT CTCCACCTCG GATTGTAGAA CACCCCTCCG ATCTGATCGT GTCAAAGGGC 
GAGCCAGCCA CCCTAAACTG CAAGGCGGAG GGTCGGCCAA CTCCCACCGT GGAGTGGTAC 
AAGGATGGAG AACGAGTTGA AACGGACAAA GATGACCCTC GATCTCACCG CATGCTCCTT 
CCCAGTGGTT CGCTCTTCTT CCTGCGTATC GTACATGGAC GCCGCAGCAA ACCTGATGAG 
GGGGCGTACG TCTGTGTGGC ACGCAACTAC TTGGGGGAAG CAGTCAGTCG AAACGCCTCG 
CTGGAAGTAG CATTGTTACG GGATGATTTC CGGCAGAACC CTACAGATGT GGTGGTCGCT 
GCTGGAGAGC CGGCCATCCT GGAGTGTGTC CCTCCCAGAG GACACCCGGA GCCCACCATC 
TACTGGAAGA AAGACAAAGT CCGAATTGAT GAGAAGGATG ACAGGATCAA GATCCGTGGA 
GGGAAGCTGA TGATCTCTAA CACCAGGAAA AGCGACGCTG GAATGTACAT CTGCGTGGGC 
ACCAACATGG TCGGCGAAAG AGACAGTGAA ACAGCCCAGG TCACTGTGTT CGAGAGGCCC 
ACGTTCCTGC GGCGTCCCAT TAACCAGGTG GTTCTGGAGG AGGAGGCTGT GGAATTTCGA 
TGTCAGGTGC AGGGAGACCC GCAGCCGTCC ATCCGCTGGA AAAAAGACGA CGTTGAAATC 
CCCCGCGGCA GGTACGATAT GAAATTTGAT AAAGATGACT TCACGCTGAG AATAAAGAAG 
GCAACAGCGA GTGATGAAGG AACCTTCACC TGTGTAGCGG AGAACCGAGT GGGCAAAATG 
GAGGCCTCCG CAACCCTCAC GGTCAGAGCT CGCCCTGTCG CTGCACCGCA GTTCGTGATC 
AGGCCGCGGG ATCAGATTGT GGCACAGGGA CGCACCGCCA GCTTCCCCTG CGAGACTAAA 
GGGAACCCGC AGCCGGCAGT CTTCTGGCAG AAAGAAGGAA GTCAGAATCT CCTGTTTCCA 
AATCAACCCC AGCAGCCCAA CAGTCGTTTC TCGGTGTCGC CCAGTGGAGA TCTAACCATC 
ACTACGGTCC AGCGGGCAGA TGCGGGCTAT TATATCTGCC AGGCCCTGAC GGTGGCGGGC 
AGCATCCTGG CCAAAGCACA GCTTGAGGTC ACAGACGTGT TGACCGATCG CCCTCCACCC 
ATCATCCGGC AGGGTCCAGC TAACCAGACT CTTGGTGTAG ACAGTGTGGC TCTTTTGAGG 
TGCCATGCAT CCGGAGAACC AGTCCCAACC ATCAGCTGGC TGAAGGACGG GGTCAGTCTG 
CTGGGCAAAG ACCCTCGGAT GTCCCTGCAA GACCTCGGCA GCCTGCAGAT CAAGGGTTTA 
CGGCTCTCTG ATTCTGGCAT TTATACTTGT GTGGCTGCAA GTTCTAGTGG GGAAACATCA 
TGGAGTGCTT TCTTGGAGGT CAAAGAGGCT GGGAGTCTTG TTGTAGTTAA AGATCGGGAT 
GAGAACGAGC TTCCAGGACC TCCTTCCAAA CCTCAGGTCA CAGATGTCAC TAAAAACAGC 
GTGTCATTAT CCTGGCAGCC AGGCCTTCCC GGAGCATCAG CCATTTCTAC ATATGTCATA 
GAAGCATTCA GCCAATCGGT GAGCAACAGC TGGCAAACTG TTGCCGATCA CGCCAAGACA 
ACGCAGTACA CCATTAAGGG TCTCCGCCCT AACACCATCT ACCTGTTCAT GGTGCGAGCG 
GTCAACGTCC AAGGCCTGAG CGACCCAAGC CCCATGTCTG AACCCGTCCG CACTCAAGAT 
ATAAGTCCTC CAGCGCAGGG TGTGGATCAC AGACATGTGC AAAAGGAGCT GGGAGAAGTT 
ATTGTGCGGC TACACAACCC TGTGGTCCTT AGCCCTACCA CCATACAGGT CACATGGACG 
GTTGACCGCC AGTCTCAGTT CATCCAGGGC TACAGGGTTT TGTACAGGCA GATGTCAGGC 
CTGTCTTCTC CAGGAGCCTG GCAAACTCAG GATGTGAAAG TCCCCTCAGA GAGGAGCATG 
GTGCTCTCGG CTCTAAAGAA AGGCATCGTC TACGAGATCA AAGTGCGTCC ATATTTCAAT 
GAGTTTCAGG GCATGGACAG CGAATCCCGA ACCGCAAGAA CAACAGAGGA AGCGCCCAGT 
GCTCCTCCTC AGCAGGTGAC TGTACTCACT GTGGGCAACC AGAACAGCAC ATCTATTAGC 
ATCTCCTGGG ACCCTCCGCC TGCAGAACAC CAGAACGGCA TCATACAGGA GTACAAGATC 
TGGTGTTTGG GGAATGAGAC CCGTTTTCAT GTGAATAAGA CGGTGGACGC AGCGATCCGC 
TCAGTGGTGG TTGGGGGTCT CCAGGCGGGG GTCCAGTATC GGGTGGAGGT GGCCGCTAGC 
ACCAGCGCCG GGGTCGGAGT GAAGAGCGAA CCACAAACCA TCATCATTGG TAAAGACTTC 
CGCGATGTGA TGATTAGCAG GAATAACAGC ATCACAGAGC AGATCACGGA TGTGGTGAAA 
CAGCCGGCCT TCATCGCGGG CATTGGCGGG GCCTGCTGGG TCATTCTGAT GGGCTTCAGC 
ATCTGGCTCT ACTGGAGGAG GAAGAAGAGA AAGGGACTCA GCAATTACGC AGTTCAGTCG 
TTCACCTTCA CCCCTGCAGT GACGTTCCAG CGTGTTGATG GAGGCTTGAT GAGCAACGGG 
AGTCGTCCTG GTCTGCTGAA CGCCAGTGAT TCGGGTTACC CGTGGCTCGC CGACTCCTGG 
CCTCCCAGCC TGCCTGTCAA CAGCGGATCT GGAGGACCCA ATGACCTCAG CTTTGGCCGT 
GGAGATGTGA TGCCCAGTGC TCAGGCTGAT AAGACGGGTA CCATGCTTTC GGATGGAGCC 
ATTTACAGCA GCATCGACTT CACCACCAAA GGCGGATTCG GTAGCCCAAG CCCAGCCTCC 
CAGGCCACGC CCTATGCCAC CACACAGATC CTCCACTCCA ACAGCATCCA TGAGCTGGCA 
GTGGATCTTC CTGAAGCCCA GTGGAAGACC TCCCTACAGG CCAAGCAGGA GATGGCCAGC 
CTGGGTTACT CACTGCCCGA CAAAAACTCC TGCAATAACA CACTCCTTTT CATTCCTGAC 
TACCGATTGG CTGACGGACT GTCTAATAGA ATGCCACACA ACCAATCGCA GGATTTCAGC 
ACCACCAGCT CCCACAACAG CTCAGATCGG AGCAACAGTC TGTCAGGTGG AAAAGGTGGT 
AAGAAGAAGA AGACCAAAGC AAACGTCAAG CCCACCAAGA TGAATGGCTC CAACTGGGCG 
AACGTTCCAC TTCCTCCTCC TCCCATCCAC CCTCTTCCTG GCACAGAGAT GGACCACTAC 
GCCTCAGAGC ACCATGATGG AGGAGGATAT GATAGTGATG GTTGGGGCCC ACCATTGCCA 
GTGCAGACGT ACCTCCATCA GGGTTTAGAG GATGAGCTGG AGGAAGAGGA AGAGCGGGTG 
CCAACTCCTC CCATTAGGGG TGTGGCTTCT TCTCCTGCTG CAGTTTCCTT TGGCAAACAG 
TCCACAGCCA CACTGAGCCC ATCTCCACGT GAAGAAATGC AGCCCATGCT CCAAGCCCAC 
CTAGATGAGC TTACCCGTGC TTATCAATTG GACATGGCTA AACAGACTTG GCACATGCAG 
GGTGGGCCTT ACCCGCCCCA GGCCCCCGCA CCACCCGTGG GCTACGTCTC CAGCACCTTA 
GTGTCTGACA TCGAGACCGA TCTCCCTGAT GAAGATGACG ATGATGAGGA GGATGAAGGA 
TACGAGATGT CGCCACCAAT TAGGGGCACT GAACACACGC CAGGCTCCAG CATGGACAAC 
TTAGACAGCT CCGTTACGGG CTCTATGGTG AACGGCTGGG GCTCGGCTTC AGAAGATGAT 
GACAGAAACT TCTCCAGCAG GAGGTCTAGT GCCGGCAGCT CTTCTGACGG CTCGCTCTTC 
ACCCATTGCA GCTTTGCGCG AGCCCTGGTG GCAGCCGCAG ACAAGGCTGG TTACCGTCTG 
GATGGCACCA GCCTGAGCAA AAGAGATAAA GGTCTACCTC ATAGACCTCG TCCCGCTAGC 
CCGTTCTCCA CAGATGGAAG CACGGTGGGC ACACACAGTC TGGGGCACCA GCGGGCAGCA 
AGGCCCACAC GTAAACACAA AGGCCCTGGC ACTGTCCCGA ACCGCCGTGA TGCCAGTGCG 
GATGATCTGC CTCCACCTCC AGAGCCTCCA CCCTGCCAGA CAGCTCGCAT CCCCGGGTCG 
AGGAACGTTC CCCATCCCTG CGACCGCAAA GCTTCCTCTC TGGAGAGGCA GCCTATGATG 
GGCCTTGAGG AAATGAGAAG TTCCTTGGAC CGGCAGGCCC GAACCTCATT GGACCGCCAC 
CAACAAGTCC AGGACAGACT GGGGTCCATA GAGAGAGGAG ATGAAAGCAG AAGAGGACAG 
AAAAATGGTC CCTTTTCAGA AGAGGCCCTA TTGCCATACA ACAAACCATC ATTCCCATCC 
CCTGGTGGGC ACAGCTCATC TGGGACGGCC TCTTCTAAAG GGTCAACGGG TCCTCGCAAA 
GGAGAAGGGA CGAGGGGACA GCACCAGCGC TCCACCACGG AACAAGGAGA AAGCAGCTAC 
ATGGGGACCA ACGCGCAGTT TTCCGGAGAG TTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017206489.1
CDS	677..5452
Translation	MFLLSPVRLTLFLLVFLDVPHPSLQGRNKGRWNKGGSRLRQEDSPPRIVEHPSDLIVSKGEPATLNCKAEGRPTPTVEWYKDGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRRSKPDEGAYVCVARNYLGEAVSRNASLEVALLRDDFRQNPTDVVVAAGEPAILECVPPRGHPEPTIYWKKDKVRIDEKDDRIKIRGGKLMISNTRKSDAGMYICVGTNMVGERDSETAQVTVFERPTFLRRPINQVVLEEEAVEFRCQVQGDPQPSIRWKKDDVEIPRGRYDMKFDKDDFTLRIKKATASDEGTFTCVAENRVGKMEASATLTVRARPVAAPQFVIRPRDQIVAQGRTASFPCETKGNPQPAVFWQKEGSQNLLFPNQPQQPNSRFSVSPSGDLTITTVQRADAGYYICQALTVAGSILAKAQLEVTDVLTDRPPPIIRQGPANQTLGVDSVALLRCHASGEPVPTISWLKDGVSLLGKDPRMSLQDLGSLQIKGLRLSDSGIYTCVAASSSGETSWSAFLEVKEAGSLVVVKDRDENELPGPPSKPQVTDVTKNSVSLSWQPGLPGASAISTYVIEAFSQSVSNSWQTVADHAKTTQYTIKGLRPNTIYLFMVRAVNVQGLSDPSPMSEPVRTQDISPPAQGVDHRHVQKELGEVIVRLHNPVVLSPTTIQVTWTVDRQSQFIQGYRVLYRQMSGLSSPGAWQTQDVKVPSERSMVLSALKKGIVYEIKVRPYFNEFQGMDSESRTARTTEEAPSAPPQQVTVLTVGNQNSTSISISWDPPPAEHQNGIIQEYKIWCLGNETRFHVNKTVDAAIRSVVVGGLQAGVQYRVEVAASTSAGVGVKSEPQTIIIGKDFRDVMISRNNSITEQITDVVKQPAFIAGIGGACWVILMGFSIWLYWRRKKRKGLSNYAVQSFTFTPAVTFQRVDGGLMSNGSRPGLLNASDSGYPWLADSWPPSLPVNSGSGGPNDLSFGRGDVMPSAQADKTGTMLSDGAIYSSIDFTTKGGFGSPSPASQATPYATTQILHSNSIHELAVDLPEAQWKTSLQAKQEMASLGYSLPDKNSCNNTLLFIPDYRLADGLSNRMPHNQSQDFSTTSSHNSSDRSNSLSGGKGGKKKKTKANVKPTKMNGSNWANVPLPPPPIHPLPGTEMDHYASEHHDGGGYDSDGWGPPLPVQTYLHQGLEDELEEEEERVPTPPIRGVASSPAAVSFGKQSTATLSPSPREEMQPMLQAHLDELTRAYQLDMAKQTWHMQGGPYPPQAPAPPVGYVSSTLVSDIETDLPDEDDDDEEDEGYEMSPPIRGTEHTPGSSMDNLDSSVTGSMVNGWGSASEDDDRNFSSRRSSAGSSSDGSLFTHCSFARALVAAADKAGYRLDGTSLSKRDKGLPHRPRPASPFSTDGSTVGTHSLGHQRAARPTRKHKGPGTVPNRRDASADDLPPPPEPPPCQTARIPGSRNVPHPCDRKASSLERQPMMGLEEMRSSLDRQARTSLDRHQQVQDRLGSIERGDESRRGQKNGPFSEEALLPYNKPSFPSPGGHSSSGTASSKGSTGPRKGEGTRGQHQRSTTEQGESSYMGTNAQFSGEL

Target ORF information:

RefSeq Version	XM_017351000.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio roundabout, axon guidance receptor, homolog 2 (Drosophila) (robo2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017351000.1

ORF Insert Sequence:

ATGTTCCTCC TCTCTCCTGT GAGACTCACT TTATTTCTGT TGGTCTTCCT GGATGTTCCT 
CATCCGTCCC TTCAGGGCAG GAATAAGGGC AGATGGAACA AGGGGGGATC CCGTCTGCGG 
CAGGAAGATT CTCCACCTCG GATTGTAGAA CACCCCTCCG ATCTGATCGT GTCAAAGGGC 
GAGCCAGCCA CCCTAAACTG CAAGGCGGAG GGTCGGCCAA CTCCCACCGT GGAGTGGTAC 
AAGGATGGAG AACGAGTTGA AACGGACAAA GATGACCCTC GATCTCACCG CATGCTCCTT 
CCCAGTGGTT CGCTCTTCTT CCTGCGTATC GTACATGGAC GCCGCAGCAA ACCTGATGAG 
GGGGCGTACG TCTGTGTGGC ACGCAACTAC TTGGGGGAAG CAGTCAGTCG AAACGCCTCG 
CTGGAAGTAG CATTGTTACG GGATGATTTC CGGCAGAACC CTACAGATGT GGTGGTCGCT 
GCTGGAGAGC CGGCCATCCT GGAGTGTGTC CCTCCCAGAG GACACCCGGA GCCCACCATC 
TACTGGAAGA AAGACAAAGT CCGAATTGAT GAGAAGGATG ACAGGATCAA GATCCGTGGA 
GGGAAGCTGA TGATCTCTAA CACCAGGAAA AGCGACGCTG GAATGTACAT CTGCGTGGGC 
ACCAACATGG TCGGCGAAAG AGACAGTGAA ACAGCCCAGG TCACTGTGTT CGAGAGGCCC 
ACGTTCCTGC GGCGTCCCAT TAACCAGGTG GTTCTGGAGG AGGAGGCTGT GGAATTTCGA 
TGTCAGGTGC AGGGAGACCC GCAGCCGTCC ATCCGCTGGA AAAAAGACGA CGTTGAAATC 
CCCCGCGGCA GGTACGATAT GAAATTTGAT AAAGATGACT TCACGCTGAG AATAAAGAAG 
GCAACAGCGA GTGATGAAGG AACCTTCACC TGTGTAGCGG AGAACCGAGT GGGCAAAATG 
GAGGCCTCCG CAACCCTCAC GGTCAGAGCT CGCCCTGTCG CTGCACCGCA GTTCGTGATC 
AGGCCGCGGG ATCAGATTGT GGCACAGGGA CGCACCGCCA GCTTCCCCTG CGAGACTAAA 
GGGAACCCGC AGCCGGCAGT CTTCTGGCAG AAAGAAGGAA GTCAGAATCT CCTGTTTCCA 
AATCAACCCC AGCAGCCCAA CAGTCGTTTC TCGGTGTCGC CCAGTGGAGA TCTAACCATC 
ACTACGGTCC AGCGGGCAGA TGCGGGCTAT TATATCTGCC AGGCCCTGAC GGTGGCGGGC 
AGCATCCTGG CCAAAGCACA GCTTGAGGTC ACAGACGTGT TGACCGATCG CCCTCCACCC 
ATCATCCGGC AGGGTCCAGC TAACCAGACT CTTGGTGTAG ACAGTGTGGC TCTTTTGAGG 
TGCCATGCAT CCGGAGAACC AGTCCCAACC ATCAGCTGGC TGAAGGACGG GGTCAGTCTG 
CTGGGCAAAG ACCCTCGGAT GTCCCTGCAA GACCTCGGCA GCCTGCAGAT CAAGGGTTTA 
CGGCTCTCTG ATTCTGGCAT TTATACTTGT GTGGCTGCAA GTTCTAGTGG GGAAACATCA 
TGGAGTGCTT TCTTGGAGGT CAAAGAGGCT GGGAGTCTTG TTGTAGTTAA AGATCGGGAT 
GAGAACGAGC TTCCAGGACC TCCTTCCAAA CCTCAGGTCA CAGATGTCAC TAAAAACAGC 
GTGTCATTAT CCTGGCAGCC AGGCCTTCCC GGAGCATCAG CCATTTCTAC ATATGTCATA 
GAAGCATTCA GCCAATCGGT GAGCAACAGC TGGCAAACTG TTGCCGATCA CGCCAAGACA 
ACGCAGTACA CCATTAAGGG TCTCCGCCCT AACACCATCT ACCTGTTCAT GGTGCGAGCG 
GTCAACGTCC AAGGCCTGAG CGACCCAAGC CCCATGTCTG AACCCGTCCG CACTCAAGAT 
ATAAGTCCTC CAGCGCAGGG TGTGGATCAC AGACATGTGC AAAAGGAGCT GGGAGAAGTT 
ATTGTGCGGC TACACAACCC TGTGGTCCTT AGCCCTACCA CCATACAGGT CACATGGACG 
GTTGACCGCC AGTCTCAGTT CATCCAGGGC TACAGGGTTT TGTACAGGCA GATGTCAGGC 
CTGTCTTCTC CAGGAGCCTG GCAAACTCAG GATGTGAAAG TCCCCTCAGA GAGGAGCATG 
GTGCTCTCGG CTCTAAAGAA AGGCATCGTC TACGAGATCA AAGTGCGTCC ATATTTCAAT 
GAGTTTCAGG GCATGGACAG CGAATCCCGA ACCGCAAGAA CAACAGAGGA AGCGCCCAGT 
GCTCCTCCTC AGCAGGTGAC TGTACTCACT GTGGGCAACC AGAACAGCAC ATCTATTAGC 
ATCTCCTGGG ACCCTCCGCC TGCAGAACAC CAGAACGGCA TCATACAGGA GTACAAGATC 
TGGTGTTTGG GGAATGAGAC CCGTTTTCAT GTGAATAAGA CGGTGGACGC AGCGATCCGC 
TCAGTGGTGG TTGGGGGTCT CCAGGCGGGG GTCCAGTATC GGGTGGAGGT GGCCGCTAGC 
ACCAGCGCCG GGGTCGGAGT GAAGAGCGAA CCACAAACCA TCATCATTGG TAAAGACTTC 
CGCGATGTGA TGATTAGCAG GAATAACAGC ATCACAGAGC AGATCACGGA TGTGGTGAAA 
CAGCCGGCCT TCATCGCGGG CATTGGCGGG GCCTGCTGGG TCATTCTGAT GGGCTTCAGC 
ATCTGGCTCT ACTGGAGGAG GAAGAAGAGA AAGGGACTCA GCAATTACGC AGTTCAGTCG 
TTCACCTTCA CCCCTGCAGT GACGTTCCAG CGTGTTGATG GAGGCTTGAT GAGCAACGGG 
AGTCGTCCTG GTCTGCTGAA CGCCAGTGAT TCGGGTTACC CGTGGCTCGC CGACTCCTGG 
CCTCCCAGCC TGCCTGTCAA CAGCGGATCT GGAGGACCCA ATGACCTCAG CTTTGGCCGT 
GGAGATGTGA TGCCCAGTGC TCAGGCTGAT AAGACGGGTA CCATGCTTTC GGATGGAGCC 
ATTTACAGCA GCATCGACTT CACCACCAAA GGCGGATTCG GTAGCCCAAG CCCAGCCTCC 
CAGGCCACGC CCTATGCCAC CACACAGATC CTCCACTCCA ACAGCATCCA TGAGCTGGCA 
GTGGATCTTC CTGAAGCCCA GTGGAAGACC TCCCTACAGG CCAAGCAGGA GATGGCCAGC 
CTGGGTTACT CACTGCCCGA CAAAAACTCC TGCAATAACA CACTCCTTTT CATTCCTGAC 
TACCGATTGG CTGACGGACT GTCTAATAGA ATGCCACACA ACCAATCGCA GGATTTCAGC 
ACCACCAGCT CCCACAACAG CTCAGATCGG AGCAACAGTC TGTCAGGTGG AAAAGGTGGT 
AAGAAGAAGA AGACCAAAGC AAACGTCAAG CCCACCAAGA TGAATGGCTC CAACTGGGCG 
AACGTTCCAC TTCCTCCTCC TCCCATCCAC CCTCTTCCTG GCACAGAGAT GGACCACTAC 
GCCTCAGAGC ACCATGATGG AGGAGGATAT GATAGTGATG GTTGGGGCCC ACCATTGCCA 
GTGCAGACGT ACCTCCATCA GGGTTTAGAG GATGAGCTGG AGGAAGAGGA AGAGCGGGTG 
CCAACTCCTC CCATTAGGGG TGTGGCTTCT TCTCCTGCTG CAGTTTCCTT TGGCAAACAG 
TCCACAGCCA CACTGAGCCC ATCTCCACGT GAAGAAATGC AGCCCATGCT CCAAGCCCAC 
CTAGATGAGC TTACCCGTGC TTATCAATTG GACATGGCTA AACAGACTTG GCACATGCAG 
GGTGGGCCTT ACCCGCCCCA GGCCCCCGCA CCACCCGTGG GCTACGTCTC CAGCACCTTA 
GTGTCTGACA TCGAGACCGA TCTCCCTGAT GAAGATGACG ATGATGAGGA GGATGAAGGA 
TACGAGATGT CGCCACCAAT TAGGGGCACT GAACACACGC CAGGCTCCAG CATGGACAAC 
TTAGACAGCT CCGTTACGGG CTCTATGGTG AACGGCTGGG GCTCGGCTTC AGAAGATGAT 
GACAGAAACT TCTCCAGCAG GAGGTCTAGT GCCGGCAGCT CTTCTGACGG CTCGCTCTTC 
ACCCATTGCA GCTTTGCGCG AGCCCTGGTG GCAGCCGCAG ACAAGGCTGG TTACCGTCTG 
GATGGCACCA GCCTGAGCAA AAGAGATAAA GGTCTACCTC ATAGACCTCG TCCCGCTAGC 
CCGTTCTCCA CAGATGGAAG CACGGTGGGC ACACACAGTC TGGGGCACCA GCGGGCAGCA 
AGGCCCACAC GTAAACACAA AGGCCCTGGC ACTGTCCCGA ACCGCCGTGA TGCCAGTGCG 
GATGATCTGC CTCCACCTCC AGAGCCTCCA CCCTGCCAGA CAGCTCGCAT CCCCGGGTCG 
AGGAACGTTC CCCATCCCTG CGACCGCAAA GCTTCCTCTC TGGAGAGGCA GCCTATGATG 
GGCCTTGAGG AAATGAGAAG TTCCTTGGAC CGGCAGGCCC GAACCTCATT GGACCGCCAC 
CAACAAGTCC AGGACAGACT GGGGTCCATA GAGAGAGGAG ATGAAAGCAG AAGAGGACAG 
AAAAATGGTC CCTTTTCAGA AGAGGCCCTA TTGCCATACA ACAAACCATC ATTCCCATCC 
CCTGGTGGGC ACAGCTCATC TGGGACGGCC TCTTCTAAAG GGTCAACGGG TCCTCGCAAA 
GGAGAAGGGA CGAGGGGACA GCACCAGCGC TCCACCACGG AACAAGGAGA AAGCAGCTAC 
ATGGGGACCA ACGCGCAGTT TTCCGGAGAG TTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

robo2 ( XM_017351000.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017206489.1 Danio rerio roundabout, axon guidance receptor, homolog 2 (Drosophila) (robo2), transcript variant X2, mRNA.

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CloneID	ODa55119
Clone ID Related Accession (Same CDS sequence)	XM_017351000.1 , XM_017351000.2
Accession Version	XM_017351000.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4776bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	roundabout homolog 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017351000.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCCTCC TCTCTCCTGT GAGACTCACT TTATTTCTGT TGGTCTTCCT GGATGTTCCT 
CATCCGTCCC TTCAGGGCAG GAATAAGGGC AGATGGAACA AGGGGGGATC CCGTCTGCGG 
CAGGAAGATT CTCCACCTCG GATTGTAGAA CACCCCTCCG ATCTGATCGT GTCAAAGGGC 
GAGCCAGCCA CCCTAAACTG CAAGGCGGAG GGTCGGCCAA CTCCCACCGT GGAGTGGTAC 
AAGGATGGAG AACGAGTTGA AACGGACAAA GATGACCCTC GATCTCACCG CATGCTCCTT 
CCCAGTGGTT CGCTCTTCTT CCTGCGTATC GTACATGGAC GCCGCAGCAA ACCTGATGAG 
GGGGCGTACG TCTGTGTGGC ACGCAACTAC TTGGGGGAAG CAGTCAGTCG AAACGCCTCG 
CTGGAAGTAG CATTGTTACG GGATGATTTC CGGCAGAACC CTACAGATGT GGTGGTCGCT 
GCTGGAGAGC CGGCCATCCT GGAGTGTGTC CCTCCCAGAG GACACCCGGA GCCCACCATC 
TACTGGAAGA AAGACAAAGT CCGAATTGAT GAGAAGGATG ACAGGATCAA GATCCGTGGA 
GGGAAGCTGA TGATCTCTAA CACCAGGAAA AGCGACGCTG GAATGTACAT CTGCGTGGGC 
ACCAACATGG TCGGCGAAAG AGACAGTGAA ACAGCCCAGG TCACTGTGTT CGAGAGGCCC 
ACGTTCCTGC GGCGTCCCAT TAACCAGGTG GTTCTGGAGG AGGAGGCTGT GGAATTTCGA 
TGTCAGGTGC AGGGAGACCC GCAGCCGTCC ATCCGCTGGA AAAAAGACGA CGTTGAAATC 
CCCCGCGGCA GGTACGATAT GAAATTTGAT AAAGATGACT TCACGCTGAG AATAAAGAAG 
GCAACAGCGA GTGATGAAGG AACCTTCACC TGTGTAGCGG AGAACCGAGT GGGCAAAATG 
GAGGCCTCCG CAACCCTCAC GGTCAGAGCT CGCCCTGTCG CTGCACCGCA GTTCGTGATC 
AGGCCGCGGG ATCAGATTGT GGCACAGGGA CGCACCGCCA GCTTCCCCTG CGAGACTAAA 
GGGAACCCGC AGCCGGCAGT CTTCTGGCAG AAAGAAGGAA GTCAGAATCT CCTGTTTCCA 
AATCAACCCC AGCAGCCCAA CAGTCGTTTC TCGGTGTCGC CCAGTGGAGA TCTAACCATC 
ACTACGGTCC AGCGGGCAGA TGCGGGCTAT TATATCTGCC AGGCCCTGAC GGTGGCGGGC 
AGCATCCTGG CCAAAGCACA GCTTGAGGTC ACAGACGTGT TGACCGATCG CCCTCCACCC 
ATCATCCGGC AGGGTCCAGC TAACCAGACT CTTGGTGTAG ACAGTGTGGC TCTTTTGAGG 
TGCCATGCAT CCGGAGAACC AGTCCCAACC ATCAGCTGGC TGAAGGACGG GGTCAGTCTG 
CTGGGCAAAG ACCCTCGGAT GTCCCTGCAA GACCTCGGCA GCCTGCAGAT CAAGGGTTTA 
CGGCTCTCTG ATTCTGGCAT TTATACTTGT GTGGCTGCAA GTTCTAGTGG GGAAACATCA 
TGGAGTGCTT TCTTGGAGGT CAAAGAGGCT GGGAGTCTTG TTGTAGTTAA AGATCGGGAT 
GAGAACGAGC TTCCAGGACC TCCTTCCAAA CCTCAGGTCA CAGATGTCAC TAAAAACAGC 
GTGTCATTAT CCTGGCAGCC AGGCCTTCCC GGAGCATCAG CCATTTCTAC ATATGTCATA 
GAAGCATTCA GCCAATCGGT GAGCAACAGC TGGCAAACTG TTGCCGATCA CGCCAAGACA 
ACGCAGTACA CCATTAAGGG TCTCCGCCCT AACACCATCT ACCTGTTCAT GGTGCGAGCG 
GTCAACGTCC AAGGCCTGAG CGACCCAAGC CCCATGTCTG AACCCGTCCG CACTCAAGAT 
ATAAGTCCTC CAGCGCAGGG TGTGGATCAC AGACATGTGC AAAAGGAGCT GGGAGAAGTT 
ATTGTGCGGC TACACAACCC TGTGGTCCTT AGCCCTACCA CCATACAGGT CACATGGACG 
GTTGACCGCC AGTCTCAGTT CATCCAGGGC TACAGGGTTT TGTACAGGCA GATGTCAGGC 
CTGTCTTCTC CAGGAGCCTG GCAAACTCAG GATGTGAAAG TCCCCTCAGA GAGGAGCATG 
GTGCTCTCGG CTCTAAAGAA AGGCATCGTC TACGAGATCA AAGTGCGTCC ATATTTCAAT 
GAGTTTCAGG GCATGGACAG CGAATCCCGA ACCGCAAGAA CAACAGAGGA AGCGCCCAGT 
GCTCCTCCTC AGCAGGTGAC TGTACTCACT GTGGGCAACC AGAACAGCAC ATCTATTAGC 
ATCTCCTGGG ACCCTCCGCC TGCAGAACAC CAGAACGGCA TCATACAGGA GTACAAGATC 
TGGTGTTTGG GGAATGAGAC CCGTTTTCAT GTGAATAAGA CGGTGGACGC AGCGATCCGC 
TCAGTGGTGG TTGGGGGTCT CCAGGCGGGG GTCCAGTATC GGGTGGAGGT GGCCGCTAGC 
ACCAGCGCCG GGGTCGGAGT GAAGAGCGAA CCACAAACCA TCATCATTGG TAAAGACTTC 
CGCGATGTGA TGATTAGCAG GAATAACAGC ATCACAGAGC AGATCACGGA TGTGGTGAAA 
CAGCCGGCCT TCATCGCGGG CATTGGCGGG GCCTGCTGGG TCATTCTGAT GGGCTTCAGC 
ATCTGGCTCT ACTGGAGGAG GAAGAAGAGA AAGGGACTCA GCAATTACGC AGTTCAGTCG 
TTCACCTTCA CCCCTGCAGT GACGTTCCAG CGTGTTGATG GAGGCTTGAT GAGCAACGGG 
AGTCGTCCTG GTCTGCTGAA CGCCAGTGAT TCGGGTTACC CGTGGCTCGC CGACTCCTGG 
CCTCCCAGCC TGCCTGTCAA CAGCGGATCT GGAGGACCCA ATGACCTCAG CTTTGGCCGT 
GGAGATGTGA TGCCCAGTGC TCAGGCTGAT AAGACGGGTA CCATGCTTTC GGATGGAGCC 
ATTTACAGCA GCATCGACTT CACCACCAAA GGCGGATTCG GTAGCCCAAG CCCAGCCTCC 
CAGGCCACGC CCTATGCCAC CACACAGATC CTCCACTCCA ACAGCATCCA TGAGCTGGCA 
GTGGATCTTC CTGAAGCCCA GTGGAAGACC TCCCTACAGG CCAAGCAGGA GATGGCCAGC 
CTGGGTTACT CACTGCCCGA CAAAAACTCC TGCAATAACA CACTCCTTTT CATTCCTGAC 
TACCGATTGG CTGACGGACT GTCTAATAGA ATGCCACACA ACCAATCGCA GGATTTCAGC 
ACCACCAGCT CCCACAACAG CTCAGATCGG AGCAACAGTC TGTCAGGTGG AAAAGGTGGT 
AAGAAGAAGA AGACCAAAGC AAACGTCAAG CCCACCAAGA TGAATGGCTC CAACTGGGCG 
AACGTTCCAC TTCCTCCTCC TCCCATCCAC CCTCTTCCTG GCACAGAGAT GGACCACTAC 
GCCTCAGAGC ACCATGATGG AGGAGGATAT GATAGTGATG GTTGGGGCCC ACCATTGCCA 
GTGCAGACGT ACCTCCATCA GGGTTTAGAG GATGAGCTGG AGGAAGAGGA AGAGCGGGTG 
CCAACTCCTC CCATTAGGGG TGTGGCTTCT TCTCCTGCTG CAGTTTCCTT TGGCAAACAG 
TCCACAGCCA CACTGAGCCC ATCTCCACGT GAAGAAATGC AGCCCATGCT CCAAGCCCAC 
CTAGATGAGC TTACCCGTGC TTATCAATTG GACATGGCTA AACAGACTTG GCACATGCAG 
GGTGGGCCTT ACCCGCCCCA GGCCCCCGCA CCACCCGTGG GCTACGTCTC CAGCACCTTA 
GTGTCTGACA TCGAGACCGA TCTCCCTGAT GAAGATGACG ATGATGAGGA GGATGAAGGA 
TACGAGATGT CGCCACCAAT TAGGGGCACT GAACACACGC CAGGCTCCAG CATGGACAAC 
TTAGACAGCT CCGTTACGGG CTCTATGGTG AACGGCTGGG GCTCGGCTTC AGAAGATGAT 
GACAGAAACT TCTCCAGCAG GAGGTCTAGT GCCGGCAGCT CTTCTGACGG CTCGCTCTTC 
ACCCATTGCA GCTTTGCGCG AGCCCTGGTG GCAGCCGCAG ACAAGGCTGG TTACCGTCTG 
GATGGCACCA GCCTGAGCAA AAGAGATAAA GGTCTACCTC ATAGACCTCG TCCCGCTAGC 
CCGTTCTCCA CAGATGGAAG CACGGTGGGC ACACACAGTC TGGGGCACCA GCGGGCAGCA 
AGGCCCACAC GTAAACACAA AGGCCCTGGC ACTGTCCCGA ACCGCCGTGA TGCCAGTGCG 
GATGATCTGC CTCCACCTCC AGAGCCTCCA CCCTGCCAGA CAGCTCGCAT CCCCGGGTCG 
AGGAACGTTC CCCATCCCTG CGACCGCAAA GCTTCCTCTC TGGAGAGGCA GCCTATGATG 
GGCCTTGAGG AAATGAGAAG TTCCTTGGAC CGGCAGGCCC GAACCTCATT GGACCGCCAC 
CAACAAGTCC AGGACAGACT GGGGTCCATA GAGAGAGGAG ATGAAAGCAG AAGAGGACAG 
AAAAATGGTC CCTTTTCAGA AGAGGCCCTA TTGCCATACA ACAAACCATC ATTCCCATCC 
CCTGGTGGGC ACAGCTCATC TGGGACGGCC TCTTCTAAAG GGTCAACGGG TCCTCGCAAA 
GGAGAAGGGA CGAGGGGACA GCACCAGCGC TCCACCACGG AACAAGGAGA AAGCAGCTAC 
ATGGGGACCA ACGCGCAGTT TTCCGGAGAG TTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017206489.1
CDS	638..5413
Translation	MFLLSPVRLTLFLLVFLDVPHPSLQGRNKGRWNKGGSRLRQEDSPPRIVEHPSDLIVSKGEPATLNCKAEGRPTPTVEWYKDGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRRSKPDEGAYVCVARNYLGEAVSRNASLEVALLRDDFRQNPTDVVVAAGEPAILECVPPRGHPEPTIYWKKDKVRIDEKDDRIKIRGGKLMISNTRKSDAGMYICVGTNMVGERDSETAQVTVFERPTFLRRPINQVVLEEEAVEFRCQVQGDPQPSIRWKKDDVEIPRGRYDMKFDKDDFTLRIKKATASDEGTFTCVAENRVGKMEASATLTVRARPVAAPQFVIRPRDQIVAQGRTASFPCETKGNPQPAVFWQKEGSQNLLFPNQPQQPNSRFSVSPSGDLTITTVQRADAGYYICQALTVAGSILAKAQLEVTDVLTDRPPPIIRQGPANQTLGVDSVALLRCHASGEPVPTISWLKDGVSLLGKDPRMSLQDLGSLQIKGLRLSDSGIYTCVAASSSGETSWSAFLEVKEAGSLVVVKDRDENELPGPPSKPQVTDVTKNSVSLSWQPGLPGASAISTYVIEAFSQSVSNSWQTVADHAKTTQYTIKGLRPNTIYLFMVRAVNVQGLSDPSPMSEPVRTQDISPPAQGVDHRHVQKELGEVIVRLHNPVVLSPTTIQVTWTVDRQSQFIQGYRVLYRQMSGLSSPGAWQTQDVKVPSERSMVLSALKKGIVYEIKVRPYFNEFQGMDSESRTARTTEEAPSAPPQQVTVLTVGNQNSTSISISWDPPPAEHQNGIIQEYKIWCLGNETRFHVNKTVDAAIRSVVVGGLQAGVQYRVEVAASTSAGVGVKSEPQTIIIGKDFRDVMISRNNSITEQITDVVKQPAFIAGIGGACWVILMGFSIWLYWRRKKRKGLSNYAVQSFTFTPAVTFQRVDGGLMSNGSRPGLLNASDSGYPWLADSWPPSLPVNSGSGGPNDLSFGRGDVMPSAQADKTGTMLSDGAIYSSIDFTTKGGFGSPSPASQATPYATTQILHSNSIHELAVDLPEAQWKTSLQAKQEMASLGYSLPDKNSCNNTLLFIPDYRLADGLSNRMPHNQSQDFSTTSSHNSSDRSNSLSGGKGGKKKKTKANVKPTKMNGSNWANVPLPPPPIHPLPGTEMDHYASEHHDGGGYDSDGWGPPLPVQTYLHQGLEDELEEEEERVPTPPIRGVASSPAAVSFGKQSTATLSPSPREEMQPMLQAHLDELTRAYQLDMAKQTWHMQGGPYPPQAPAPPVGYVSSTLVSDIETDLPDEDDDDEEDEGYEMSPPIRGTEHTPGSSMDNLDSSVTGSMVNGWGSASEDDDRNFSSRRSSAGSSSDGSLFTHCSFARALVAAADKAGYRLDGTSLSKRDKGLPHRPRPASPFSTDGSTVGTHSLGHQRAARPTRKHKGPGTVPNRRDASADDLPPPPEPPPCQTARIPGSRNVPHPCDRKASSLERQPMMGLEEMRSSLDRQARTSLDRHQQVQDRLGSIERGDESRRGQKNGPFSEEALLPYNKPSFPSPGGHSSSGTASSKGSTGPRKGEGTRGQHQRSTTEQGESSYMGTNAQFSGEL

Target ORF information:

RefSeq Version	XM_017351000.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio roundabout, axon guidance receptor, homolog 2 (Drosophila) (robo2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017351000.2

ORF Insert Sequence:

ATGTTCCTCC TCTCTCCTGT GAGACTCACT TTATTTCTGT TGGTCTTCCT GGATGTTCCT 
CATCCGTCCC TTCAGGGCAG GAATAAGGGC AGATGGAACA AGGGGGGATC CCGTCTGCGG 
CAGGAAGATT CTCCACCTCG GATTGTAGAA CACCCCTCCG ATCTGATCGT GTCAAAGGGC 
GAGCCAGCCA CCCTAAACTG CAAGGCGGAG GGTCGGCCAA CTCCCACCGT GGAGTGGTAC 
AAGGATGGAG AACGAGTTGA AACGGACAAA GATGACCCTC GATCTCACCG CATGCTCCTT 
CCCAGTGGTT CGCTCTTCTT CCTGCGTATC GTACATGGAC GCCGCAGCAA ACCTGATGAG 
GGGGCGTACG TCTGTGTGGC ACGCAACTAC TTGGGGGAAG CAGTCAGTCG AAACGCCTCG 
CTGGAAGTAG CATTGTTACG GGATGATTTC CGGCAGAACC CTACAGATGT GGTGGTCGCT 
GCTGGAGAGC CGGCCATCCT GGAGTGTGTC CCTCCCAGAG GACACCCGGA GCCCACCATC 
TACTGGAAGA AAGACAAAGT CCGAATTGAT GAGAAGGATG ACAGGATCAA GATCCGTGGA 
GGGAAGCTGA TGATCTCTAA CACCAGGAAA AGCGACGCTG GAATGTACAT CTGCGTGGGC 
ACCAACATGG TCGGCGAAAG AGACAGTGAA ACAGCCCAGG TCACTGTGTT CGAGAGGCCC 
ACGTTCCTGC GGCGTCCCAT TAACCAGGTG GTTCTGGAGG AGGAGGCTGT GGAATTTCGA 
TGTCAGGTGC AGGGAGACCC GCAGCCGTCC ATCCGCTGGA AAAAAGACGA CGTTGAAATC 
CCCCGCGGCA GGTACGATAT GAAATTTGAT AAAGATGACT TCACGCTGAG AATAAAGAAG 
GCAACAGCGA GTGATGAAGG AACCTTCACC TGTGTAGCGG AGAACCGAGT GGGCAAAATG 
GAGGCCTCCG CAACCCTCAC GGTCAGAGCT CGCCCTGTCG CTGCACCGCA GTTCGTGATC 
AGGCCGCGGG ATCAGATTGT GGCACAGGGA CGCACCGCCA GCTTCCCCTG CGAGACTAAA 
GGGAACCCGC AGCCGGCAGT CTTCTGGCAG AAAGAAGGAA GTCAGAATCT CCTGTTTCCA 
AATCAACCCC AGCAGCCCAA CAGTCGTTTC TCGGTGTCGC CCAGTGGAGA TCTAACCATC 
ACTACGGTCC AGCGGGCAGA TGCGGGCTAT TATATCTGCC AGGCCCTGAC GGTGGCGGGC 
AGCATCCTGG CCAAAGCACA GCTTGAGGTC ACAGACGTGT TGACCGATCG CCCTCCACCC 
ATCATCCGGC AGGGTCCAGC TAACCAGACT CTTGGTGTAG ACAGTGTGGC TCTTTTGAGG 
TGCCATGCAT CCGGAGAACC AGTCCCAACC ATCAGCTGGC TGAAGGACGG GGTCAGTCTG 
CTGGGCAAAG ACCCTCGGAT GTCCCTGCAA GACCTCGGCA GCCTGCAGAT CAAGGGTTTA 
CGGCTCTCTG ATTCTGGCAT TTATACTTGT GTGGCTGCAA GTTCTAGTGG GGAAACATCA 
TGGAGTGCTT TCTTGGAGGT CAAAGAGGCT GGGAGTCTTG TTGTAGTTAA AGATCGGGAT 
GAGAACGAGC TTCCAGGACC TCCTTCCAAA CCTCAGGTCA CAGATGTCAC TAAAAACAGC 
GTGTCATTAT CCTGGCAGCC AGGCCTTCCC GGAGCATCAG CCATTTCTAC ATATGTCATA 
GAAGCATTCA GCCAATCGGT GAGCAACAGC TGGCAAACTG TTGCCGATCA CGCCAAGACA 
ACGCAGTACA CCATTAAGGG TCTCCGCCCT AACACCATCT ACCTGTTCAT GGTGCGAGCG 
GTCAACGTCC AAGGCCTGAG CGACCCAAGC CCCATGTCTG AACCCGTCCG CACTCAAGAT 
ATAAGTCCTC CAGCGCAGGG TGTGGATCAC AGACATGTGC AAAAGGAGCT GGGAGAAGTT 
ATTGTGCGGC TACACAACCC TGTGGTCCTT AGCCCTACCA CCATACAGGT CACATGGACG 
GTTGACCGCC AGTCTCAGTT CATCCAGGGC TACAGGGTTT TGTACAGGCA GATGTCAGGC 
CTGTCTTCTC CAGGAGCCTG GCAAACTCAG GATGTGAAAG TCCCCTCAGA GAGGAGCATG 
GTGCTCTCGG CTCTAAAGAA AGGCATCGTC TACGAGATCA AAGTGCGTCC ATATTTCAAT 
GAGTTTCAGG GCATGGACAG CGAATCCCGA ACCGCAAGAA CAACAGAGGA AGCGCCCAGT 
GCTCCTCCTC AGCAGGTGAC TGTACTCACT GTGGGCAACC AGAACAGCAC ATCTATTAGC 
ATCTCCTGGG ACCCTCCGCC TGCAGAACAC CAGAACGGCA TCATACAGGA GTACAAGATC 
TGGTGTTTGG GGAATGAGAC CCGTTTTCAT GTGAATAAGA CGGTGGACGC AGCGATCCGC 
TCAGTGGTGG TTGGGGGTCT CCAGGCGGGG GTCCAGTATC GGGTGGAGGT GGCCGCTAGC 
ACCAGCGCCG GGGTCGGAGT GAAGAGCGAA CCACAAACCA TCATCATTGG TAAAGACTTC 
CGCGATGTGA TGATTAGCAG GAATAACAGC ATCACAGAGC AGATCACGGA TGTGGTGAAA 
CAGCCGGCCT TCATCGCGGG CATTGGCGGG GCCTGCTGGG TCATTCTGAT GGGCTTCAGC 
ATCTGGCTCT ACTGGAGGAG GAAGAAGAGA AAGGGACTCA GCAATTACGC AGTTCAGTCG 
TTCACCTTCA CCCCTGCAGT GACGTTCCAG CGTGTTGATG GAGGCTTGAT GAGCAACGGG 
AGTCGTCCTG GTCTGCTGAA CGCCAGTGAT TCGGGTTACC CGTGGCTCGC CGACTCCTGG 
CCTCCCAGCC TGCCTGTCAA CAGCGGATCT GGAGGACCCA ATGACCTCAG CTTTGGCCGT 
GGAGATGTGA TGCCCAGTGC TCAGGCTGAT AAGACGGGTA CCATGCTTTC GGATGGAGCC 
ATTTACAGCA GCATCGACTT CACCACCAAA GGCGGATTCG GTAGCCCAAG CCCAGCCTCC 
CAGGCCACGC CCTATGCCAC CACACAGATC CTCCACTCCA ACAGCATCCA TGAGCTGGCA 
GTGGATCTTC CTGAAGCCCA GTGGAAGACC TCCCTACAGG CCAAGCAGGA GATGGCCAGC 
CTGGGTTACT CACTGCCCGA CAAAAACTCC TGCAATAACA CACTCCTTTT CATTCCTGAC 
TACCGATTGG CTGACGGACT GTCTAATAGA ATGCCACACA ACCAATCGCA GGATTTCAGC 
ACCACCAGCT CCCACAACAG CTCAGATCGG AGCAACAGTC TGTCAGGTGG AAAAGGTGGT 
AAGAAGAAGA AGACCAAAGC AAACGTCAAG CCCACCAAGA TGAATGGCTC CAACTGGGCG 
AACGTTCCAC TTCCTCCTCC TCCCATCCAC CCTCTTCCTG GCACAGAGAT GGACCACTAC 
GCCTCAGAGC ACCATGATGG AGGAGGATAT GATAGTGATG GTTGGGGCCC ACCATTGCCA 
GTGCAGACGT ACCTCCATCA GGGTTTAGAG GATGAGCTGG AGGAAGAGGA AGAGCGGGTG 
CCAACTCCTC CCATTAGGGG TGTGGCTTCT TCTCCTGCTG CAGTTTCCTT TGGCAAACAG 
TCCACAGCCA CACTGAGCCC ATCTCCACGT GAAGAAATGC AGCCCATGCT CCAAGCCCAC 
CTAGATGAGC TTACCCGTGC TTATCAATTG GACATGGCTA AACAGACTTG GCACATGCAG 
GGTGGGCCTT ACCCGCCCCA GGCCCCCGCA CCACCCGTGG GCTACGTCTC CAGCACCTTA 
GTGTCTGACA TCGAGACCGA TCTCCCTGAT GAAGATGACG ATGATGAGGA GGATGAAGGA 
TACGAGATGT CGCCACCAAT TAGGGGCACT GAACACACGC CAGGCTCCAG CATGGACAAC 
TTAGACAGCT CCGTTACGGG CTCTATGGTG AACGGCTGGG GCTCGGCTTC AGAAGATGAT 
GACAGAAACT TCTCCAGCAG GAGGTCTAGT GCCGGCAGCT CTTCTGACGG CTCGCTCTTC 
ACCCATTGCA GCTTTGCGCG AGCCCTGGTG GCAGCCGCAG ACAAGGCTGG TTACCGTCTG 
GATGGCACCA GCCTGAGCAA AAGAGATAAA GGTCTACCTC ATAGACCTCG TCCCGCTAGC 
CCGTTCTCCA CAGATGGAAG CACGGTGGGC ACACACAGTC TGGGGCACCA GCGGGCAGCA 
AGGCCCACAC GTAAACACAA AGGCCCTGGC ACTGTCCCGA ACCGCCGTGA TGCCAGTGCG 
GATGATCTGC CTCCACCTCC AGAGCCTCCA CCCTGCCAGA CAGCTCGCAT CCCCGGGTCG 
AGGAACGTTC CCCATCCCTG CGACCGCAAA GCTTCCTCTC TGGAGAGGCA GCCTATGATG 
GGCCTTGAGG AAATGAGAAG TTCCTTGGAC CGGCAGGCCC GAACCTCATT GGACCGCCAC 
CAACAAGTCC AGGACAGACT GGGGTCCATA GAGAGAGGAG ATGAAAGCAG AAGAGGACAG 
AAAAATGGTC CCTTTTCAGA AGAGGCCCTA TTGCCATACA ACAAACCATC ATTCCCATCC 
CCTGGTGGGC ACAGCTCATC TGGGACGGCC TCTTCTAAAG GGTCAACGGG TCCTCGCAAA 
GGAGAAGGGA CGAGGGGACA GCACCAGCGC TCCACCACGG AACAAGGAGA AAGCAGCTAC 
ATGGGGACCA ACGCGCAGTT TTCCGGAGAG TTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

robo2 ( XM_017351001.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017206490.1 Danio rerio roundabout, axon guidance receptor, homolog 2 (Drosophila) (robo2), transcript variant X4, mRNA.

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CloneID	ODa55120
Clone ID Related Accession (Same CDS sequence)	XM_017351001.1
Accession Version	XM_017351001.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4773bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	roundabout homolog 2 isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCCTCC TCTCTCCTGT GAGACTCACT TTATTTCTGT TGGTCTTCCT GGATGTTCCT 
CATCCGTCCC TTCAGGGCAG GAATAAGGGC AGATGGAACA AGGGGGGATC CCGTCTGCGG 
CAGGAAGATT CTCCACCTCG GATTGTAGAA CACCCCTCCG ATCTGATCGT GTCAAAGGGC 
GAGCCAGCCA CCCTAAACTG CAAGGCGGAG GGTCGGCCAA CTCCCACCGT GGAGTGGTAC 
AAGGATGGAG AACGAGTTGA AACGGACAAA GATGACCCTC GATCTCACCG CATGCTCCTT 
CCCAGTGGTT CGCTCTTCTT CCTGCGTATC GTACATGGAC GCCGCAGCAA ACCTGATGAG 
GGGGCGTACG TCTGTGTGGC ACGCAACTAC TTGGGGGAAG CAGTCAGTCG AAACGCCTCG 
CTGGAAGTAG CATTGTTACG GGATGATTTC CGGCAGAACC CTACAGATGT GGTGGTCGCT 
GCTGGAGAGC CGGCCATCCT GGAGTGTGTC CCTCCCAGAG GACACCCGGA GCCCACCATC 
TACTGGAAGA AAGACAAAGT CCGAATTGAT GAGAAGGATG ACAGGATCAA GATCCGTGGA 
GGGAAGCTGA TGATCTCTAA CACCAGGAAA AGCGACGCTG GAATGTACAT CTGCGTGGGC 
ACCAACATGG TCGGCGAAAG AGACAGTGAA ACAGCCCAGG TCACTGTGTT CGAGAGGCCC 
ACGTTCCTGC GGCGTCCCAT TAACCAGGTG GTTCTGGAGG AGGAGGCTGT GGAATTTCGA 
TGTCAGGTGC AGGGAGACCC GCAGCCGTCC ATCCGCTGGA AAAAAGACGA CGTTGAAATC 
CCCCGCGGCA GGTACGATAT GAAATTTGAT AAAGATGACT TCACGCTGAG AATAAAGAAG 
GCAACAGCGA GTGATGAAGG AACCTTCACC TGTGTAGCGG AGAACCGAGT GGGCAAAATG 
GAGGCCTCCG CAACCCTCAC GGTCAGAGCT CGCCCTGTCG CTGCACCGCA GTTCGTGATC 
AGGCCGCGGG ATCAGATTGT GGCACAGGGA CGCACCGCCA GCTTCCCCTG CGAGACTAAA 
GGGAACCCGC AGCCGGCAGT CTTCTGGCAG AAAGAAGGAA GTCAGAATCT CCTGTTTCCA 
AATCAACCCC AGCAGCCCAA CAGTCGTTTC TCGGTGTCGC CCAGTGGAGA TCTAACCATC 
ACTACGGTCC AGCGGGCAGA TGCGGGCTAT TATATCTGCC AGGCCCTGAC GGTGGCGGGC 
AGCATCCTGG CCAAAGCACA GCTTGAGGTC ACAGACGTGT TGACCGATCG CCCTCCACCC 
ATCATCCGGC AGGGTCCAGC TAACCAGACT CTTGGTGTAG ACAGTGTGGC TCTTTTGAGG 
TGCCATGCAT CCGGAGAACC AGTCCCAACC ATCAGCTGGC TGAAGGACGG GGTCAGTCTG 
CTGGGCAAAG ACCCTCGGAT GTCCCTGCAA GACCTCGGCA GCCTGCAGAT CAAGGGTTTA 
CGGCTCTCTG ATTCTGGCAT TTATACTTGT GTGGCTGCAA GTTCTAGTGG GGAAACATCA 
TGGAGTGCTT TCTTGGAGGT CAAAGAGGCT GGGAGTCTTG TTGTAGTTAA AGATCGGGAT 
GAGAACGAGC TTCCAGGACC TCCTTCCAAA CCTCAGGTCA CAGATGTCAC TAAAAACAGC 
GTGTCATTAT CCTGGCAGCC AGGCCTTCCC GGAGCATCAG CCATTTCTAC ATATGTCATA 
GAAGCATTCA GCCAATCGGT GAGCAACAGC TGGCAAACTG TTGCCGATCA CGCCAAGACA 
ACGCAGTACA CCATTAAGGG TCTCCGCCCT AACACCATCT ACCTGTTCAT GGTGCGAGCG 
GTCAACGTCC AAGGCCTGAG CGACCCAAGC CCCATGTCTG AACCCGTCCG CACTCAAGAT 
ATAAGTCCTC CAGCGCAGGG TGTGGATCAC AGACATGTGC AAAAGGAGCT GGGAGAAGTT 
ATTGTGCGGC TACACAACCC TGTGGTCCTT AGCCCTACCA CCATACAGGT CACATGGACG 
GTTGACCGCC AGTCTCAGTT CATCCAGGGC TACAGGGTTT TGTACAGGCA GATGTCAGGC 
CTGTCTTCTC CAGGAGCCTG GCAAACTCAG GATGTGAAAG TCCCCTCAGA GAGGAGCATG 
GTGCTCTCGG CTCTAAAGAA AGGCATCGTC TACGAGATCA AAGTGCGTCC ATATTTCAAT 
GAGTTTCAGG GCATGGACAG CGAATCCCGA ACCGCAAGAA CAACAGAGGA AGCGCCCAGT 
GCTCCTCCTC AGCAGGTGAC TGTACTCACT GTGGGCAACC AGAACAGCAC ATCTATTAGC 
ATCTCCTGGG ACCCTCCGCC TGCAGAACAC CAGAACGGCA TCATACAGGA GTACAAGATC 
TGGTGTTTGG GGAATGAGAC CCGTTTTCAT GTGAATAAGA CGGTGGACGC AGCGATCCGC 
TCAGTGGTGG TTGGGGGTCT CCAGGCGGGG GTCCAGTATC GGGTGGAGGT GGCCGCTAGC 
ACCAGCGCCG GGGTCGGAGT GAAGAGCGAA CCACAAACCA TCATCATTGG TAAAGACTTC 
CGCGATGTGA TGATTAGCAG GAATAACAGC ATCACAGAGC AGATCACGGA TGTGGTGAAA 
CAGCCGGCCT TCATCGCGGG CATTGGCGGG GCCTGCTGGG TCATTCTGAT GGGCTTCAGC 
ATCTGGCTCT ACTGGAGGAG GAAGAAGAGA AAGGGACTCA GCAATTACGC AGTGACGTTC 
CAGCGTGTTG ATGGAGGCTT GATGAGCAAC GGGAGTCGTC CTGGTCTGCT GAACGCCAGT 
GATTCGGGTT ACCCGTGGCT CGCCGACTCC TGGCCTCCCA GCCTGCCTGT CAACAGCGGA 
TCTGGAGGAC CCAATGACCT CAGCTTTGGC CGTGGAGATG TGATGCCCAG TGCTCAGGCT 
GATAAGACGG GTACCATGCT TTCGGATGGA GCCATTTACA GCAGCATCGA CTTCACCACC 
AAAGGCGGAT TCGGTAGCCC AAGCCCAGCC TCCCAGGCCA CGCCCTATGC CACCACACAG 
ATCCTCCACT CCAACAGCAT CCATGAGCTG GCAGTGGATC TTCCTGAAGC CCAGTGGAAG 
ACCTCCCTAC AGGCCAAGCA GGAGATGGCC AGCCTGGGTT ACTCACTGCC CGACAAAAAC 
TCCTGCAATA ACACACTCCT TTTCATTCCT GACTACCGAT TGGCTGACGG ACTGTCTAAT 
AGAATGCCAC ACAACCAATC GCAGGATTTC AGCACCACCA GCTCCCACAA CAGCTCAGAT 
CGGAGCAACA GTCTGTCAGG TGGAAAAGGT GGTAAGAAGA AGAAGACCAA AGCAAACGTC 
AAGCCCACCA AGATGAATGG CTCCAACTGG GCGAACGTTC CACTTCCTCC TCCTCCCATC 
CACCCTCTTC CTGGCACAGA GATGGACCAC TACGCCTCAG AGCACCATGA TGGAGGAGGA 
TATGATAGTG ATGGTTGGGG CCCACCATTG CCAGTGCAGA CGTACCTCCA TCAGGGTTTA 
GAGGATGAGC TGGAGGAAGA GGAAGAGCGG GTGCCAACTC CTCCCATTAG GGGTGTGGCT 
TCTTCTCCTG CTGCAGTTTC CTTTGGCAAA CAGTCCACAG CCACACTGAG CCCATCTCCA 
CGTGAAGAAA TGCAGCCCAT GCTCCAAGCC CACCTAGATG AGCTTACCCG TGCTTATCAA 
TTGGACATGG CTAAACAGAC TTGGCACATG CAGGGTGGGC CTTACCCGCC CCAGGCCCCC 
GCACCACCCG TGGGCTACGT CTCCAGCACC TTAGTGTCTG ACATCGAGAC CGATCTCCCT 
GATGAAGATG ACGATGATGA GGAGGATGAA GGATACGAGA TGTCGCCACC AATTAGGGGC 
ACTGAACACA CGCCAGGCTC CAGCATGGAC AACTTAGACA GCTCCGTTAC GGGCTCTATG 
GTGAACGGCT GGGGCTCGGC TTCAGAAGAT GATGACAGAA ACTTCTCCAG CAGGAGGTCT 
AGTGCCGGCA GCTCTTCTGA CGGCTCGCTC TTCACCCATT GCAGCTTTGC GCGAGCCCTG 
GTGGCAGCCG CAGACAAGGC TGGTTACCGT CTGGATGGCA CCAGCCTGAG CAAAAGAGAT 
AAAGGTCTAC CTCATAGACC TCGTCCCGCT AGCCCGTTCT CCACAGATGG AAGCACGGTG 
GGCACACACA GTCTGGGGCA CCAGCGGGCA GCAAGGCCCA CACGTAAACA CAAAGGCCCT 
GGCACTGTCC CGAACCGCCG TGATGCCAGT GCGGATGATC TGCCTCCACC TCCAGAGCCT 
CCACCCTGCC AGACAGCTCG CATCCCCGGG TCGAGGAACG TTCCCCATCC CTGCGACCGC 
AAAGCTTCCT CTCTGGAGAG GCAGCCTATG ATGGGCCTTG AGGAAATGAG AAGTTCCTTG 
GACCGGCAGG CCCGAACCTC ATTGGACCGC CACCAACAAG TCCAGGACAG ACTGGGGTCC 
ATAGAGAGAG GAGATGAAAG CAGAAGAGGA CAGAAAAATG GTCCCTTTTC AGAAGAGGCC 
CTATTGCCAT ACAACAAACC ATCATTCCCA TCCCCTGGTG GGCACAGCTC ATCTGGGACG 
GCCTCTTCTA AAGGGTCAAC GGGTCCTCGC AAAGGAGAAG GGACGAGGGG ACAGCACCAG 
CGCTCCACCA CGGAACAAGG AGAAAGCAGC TACATGGGGA CCAACGCGCA GTTTTCCGGA 
GAGTTATGTG AGTCTGAACA AGTTCAAGAG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017206490.1
CDS	680..5452
Translation	MFLLSPVRLTLFLLVFLDVPHPSLQGRNKGRWNKGGSRLRQEDSPPRIVEHPSDLIVSKGEPATLNCKAEGRPTPTVEWYKDGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRRSKPDEGAYVCVARNYLGEAVSRNASLEVALLRDDFRQNPTDVVVAAGEPAILECVPPRGHPEPTIYWKKDKVRIDEKDDRIKIRGGKLMISNTRKSDAGMYICVGTNMVGERDSETAQVTVFERPTFLRRPINQVVLEEEAVEFRCQVQGDPQPSIRWKKDDVEIPRGRYDMKFDKDDFTLRIKKATASDEGTFTCVAENRVGKMEASATLTVRARPVAAPQFVIRPRDQIVAQGRTASFPCETKGNPQPAVFWQKEGSQNLLFPNQPQQPNSRFSVSPSGDLTITTVQRADAGYYICQALTVAGSILAKAQLEVTDVLTDRPPPIIRQGPANQTLGVDSVALLRCHASGEPVPTISWLKDGVSLLGKDPRMSLQDLGSLQIKGLRLSDSGIYTCVAASSSGETSWSAFLEVKEAGSLVVVKDRDENELPGPPSKPQVTDVTKNSVSLSWQPGLPGASAISTYVIEAFSQSVSNSWQTVADHAKTTQYTIKGLRPNTIYLFMVRAVNVQGLSDPSPMSEPVRTQDISPPAQGVDHRHVQKELGEVIVRLHNPVVLSPTTIQVTWTVDRQSQFIQGYRVLYRQMSGLSSPGAWQTQDVKVPSERSMVLSALKKGIVYEIKVRPYFNEFQGMDSESRTARTTEEAPSAPPQQVTVLTVGNQNSTSISISWDPPPAEHQNGIIQEYKIWCLGNETRFHVNKTVDAAIRSVVVGGLQAGVQYRVEVAASTSAGVGVKSEPQTIIIGKDFRDVMISRNNSITEQITDVVKQPAFIAGIGGACWVILMGFSIWLYWRRKKRKGLSNYAVTFQRVDGGLMSNGSRPGLLNASDSGYPWLADSWPPSLPVNSGSGGPNDLSFGRGDVMPSAQADKTGTMLSDGAIYSSIDFTTKGGFGSPSPASQATPYATTQILHSNSIHELAVDLPEAQWKTSLQAKQEMASLGYSLPDKNSCNNTLLFIPDYRLADGLSNRMPHNQSQDFSTTSSHNSSDRSNSLSGGKGGKKKKTKANVKPTKMNGSNWANVPLPPPPIHPLPGTEMDHYASEHHDGGGYDSDGWGPPLPVQTYLHQGLEDELEEEEERVPTPPIRGVASSPAAVSFGKQSTATLSPSPREEMQPMLQAHLDELTRAYQLDMAKQTWHMQGGPYPPQAPAPPVGYVSSTLVSDIETDLPDEDDDDEEDEGYEMSPPIRGTEHTPGSSMDNLDSSVTGSMVNGWGSASEDDDRNFSSRRSSAGSSSDGSLFTHCSFARALVAAADKAGYRLDGTSLSKRDKGLPHRPRPASPFSTDGSTVGTHSLGHQRAARPTRKHKGPGTVPNRRDASADDLPPPPEPPPCQTARIPGSRNVPHPCDRKASSLERQPMMGLEEMRSSLDRQARTSLDRHQQVQDRLGSIERGDESRRGQKNGPFSEEALLPYNKPSFPSPGGHSSSGTASSKGSTGPRKGEGTRGQHQRSTTEQGESSYMGTNAQFSGELCESEQVQE

Target ORF information:

RefSeq Version	XM_017351001.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio roundabout, axon guidance receptor, homolog 2 (Drosophila) (robo2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017351001.1

ORF Insert Sequence:

ATGTTCCTCC TCTCTCCTGT GAGACTCACT TTATTTCTGT TGGTCTTCCT GGATGTTCCT 
CATCCGTCCC TTCAGGGCAG GAATAAGGGC AGATGGAACA AGGGGGGATC CCGTCTGCGG 
CAGGAAGATT CTCCACCTCG GATTGTAGAA CACCCCTCCG ATCTGATCGT GTCAAAGGGC 
GAGCCAGCCA CCCTAAACTG CAAGGCGGAG GGTCGGCCAA CTCCCACCGT GGAGTGGTAC 
AAGGATGGAG AACGAGTTGA AACGGACAAA GATGACCCTC GATCTCACCG CATGCTCCTT 
CCCAGTGGTT CGCTCTTCTT CCTGCGTATC GTACATGGAC GCCGCAGCAA ACCTGATGAG 
GGGGCGTACG TCTGTGTGGC ACGCAACTAC TTGGGGGAAG CAGTCAGTCG AAACGCCTCG 
CTGGAAGTAG CATTGTTACG GGATGATTTC CGGCAGAACC CTACAGATGT GGTGGTCGCT 
GCTGGAGAGC CGGCCATCCT GGAGTGTGTC CCTCCCAGAG GACACCCGGA GCCCACCATC 
TACTGGAAGA AAGACAAAGT CCGAATTGAT GAGAAGGATG ACAGGATCAA GATCCGTGGA 
GGGAAGCTGA TGATCTCTAA CACCAGGAAA AGCGACGCTG GAATGTACAT CTGCGTGGGC 
ACCAACATGG TCGGCGAAAG AGACAGTGAA ACAGCCCAGG TCACTGTGTT CGAGAGGCCC 
ACGTTCCTGC GGCGTCCCAT TAACCAGGTG GTTCTGGAGG AGGAGGCTGT GGAATTTCGA 
TGTCAGGTGC AGGGAGACCC GCAGCCGTCC ATCCGCTGGA AAAAAGACGA CGTTGAAATC 
CCCCGCGGCA GGTACGATAT GAAATTTGAT AAAGATGACT TCACGCTGAG AATAAAGAAG 
GCAACAGCGA GTGATGAAGG AACCTTCACC TGTGTAGCGG AGAACCGAGT GGGCAAAATG 
GAGGCCTCCG CAACCCTCAC GGTCAGAGCT CGCCCTGTCG CTGCACCGCA GTTCGTGATC 
AGGCCGCGGG ATCAGATTGT GGCACAGGGA CGCACCGCCA GCTTCCCCTG CGAGACTAAA 
GGGAACCCGC AGCCGGCAGT CTTCTGGCAG AAAGAAGGAA GTCAGAATCT CCTGTTTCCA 
AATCAACCCC AGCAGCCCAA CAGTCGTTTC TCGGTGTCGC CCAGTGGAGA TCTAACCATC 
ACTACGGTCC AGCGGGCAGA TGCGGGCTAT TATATCTGCC AGGCCCTGAC GGTGGCGGGC 
AGCATCCTGG CCAAAGCACA GCTTGAGGTC ACAGACGTGT TGACCGATCG CCCTCCACCC 
ATCATCCGGC AGGGTCCAGC TAACCAGACT CTTGGTGTAG ACAGTGTGGC TCTTTTGAGG 
TGCCATGCAT CCGGAGAACC AGTCCCAACC ATCAGCTGGC TGAAGGACGG GGTCAGTCTG 
CTGGGCAAAG ACCCTCGGAT GTCCCTGCAA GACCTCGGCA GCCTGCAGAT CAAGGGTTTA 
CGGCTCTCTG ATTCTGGCAT TTATACTTGT GTGGCTGCAA GTTCTAGTGG GGAAACATCA 
TGGAGTGCTT TCTTGGAGGT CAAAGAGGCT GGGAGTCTTG TTGTAGTTAA AGATCGGGAT 
GAGAACGAGC TTCCAGGACC TCCTTCCAAA CCTCAGGTCA CAGATGTCAC TAAAAACAGC 
GTGTCATTAT CCTGGCAGCC AGGCCTTCCC GGAGCATCAG CCATTTCTAC ATATGTCATA 
GAAGCATTCA GCCAATCGGT GAGCAACAGC TGGCAAACTG TTGCCGATCA CGCCAAGACA 
ACGCAGTACA CCATTAAGGG TCTCCGCCCT AACACCATCT ACCTGTTCAT GGTGCGAGCG 
GTCAACGTCC AAGGCCTGAG CGACCCAAGC CCCATGTCTG AACCCGTCCG CACTCAAGAT 
ATAAGTCCTC CAGCGCAGGG TGTGGATCAC AGACATGTGC AAAAGGAGCT GGGAGAAGTT 
ATTGTGCGGC TACACAACCC TGTGGTCCTT AGCCCTACCA CCATACAGGT CACATGGACG 
GTTGACCGCC AGTCTCAGTT CATCCAGGGC TACAGGGTTT TGTACAGGCA GATGTCAGGC 
CTGTCTTCTC CAGGAGCCTG GCAAACTCAG GATGTGAAAG TCCCCTCAGA GAGGAGCATG 
GTGCTCTCGG CTCTAAAGAA AGGCATCGTC TACGAGATCA AAGTGCGTCC ATATTTCAAT 
GAGTTTCAGG GCATGGACAG CGAATCCCGA ACCGCAAGAA CAACAGAGGA AGCGCCCAGT 
GCTCCTCCTC AGCAGGTGAC TGTACTCACT GTGGGCAACC AGAACAGCAC ATCTATTAGC 
ATCTCCTGGG ACCCTCCGCC TGCAGAACAC CAGAACGGCA TCATACAGGA GTACAAGATC 
TGGTGTTTGG GGAATGAGAC CCGTTTTCAT GTGAATAAGA CGGTGGACGC AGCGATCCGC 
TCAGTGGTGG TTGGGGGTCT CCAGGCGGGG GTCCAGTATC GGGTGGAGGT GGCCGCTAGC 
ACCAGCGCCG GGGTCGGAGT GAAGAGCGAA CCACAAACCA TCATCATTGG TAAAGACTTC 
CGCGATGTGA TGATTAGCAG GAATAACAGC ATCACAGAGC AGATCACGGA TGTGGTGAAA 
CAGCCGGCCT TCATCGCGGG CATTGGCGGG GCCTGCTGGG TCATTCTGAT GGGCTTCAGC 
ATCTGGCTCT ACTGGAGGAG GAAGAAGAGA AAGGGACTCA GCAATTACGC AGTGACGTTC 
CAGCGTGTTG ATGGAGGCTT GATGAGCAAC GGGAGTCGTC CTGGTCTGCT GAACGCCAGT 
GATTCGGGTT ACCCGTGGCT CGCCGACTCC TGGCCTCCCA GCCTGCCTGT CAACAGCGGA 
TCTGGAGGAC CCAATGACCT CAGCTTTGGC CGTGGAGATG TGATGCCCAG TGCTCAGGCT 
GATAAGACGG GTACCATGCT TTCGGATGGA GCCATTTACA GCAGCATCGA CTTCACCACC 
AAAGGCGGAT TCGGTAGCCC AAGCCCAGCC TCCCAGGCCA CGCCCTATGC CACCACACAG 
ATCCTCCACT CCAACAGCAT CCATGAGCTG GCAGTGGATC TTCCTGAAGC CCAGTGGAAG 
ACCTCCCTAC AGGCCAAGCA GGAGATGGCC AGCCTGGGTT ACTCACTGCC CGACAAAAAC 
TCCTGCAATA ACACACTCCT TTTCATTCCT GACTACCGAT TGGCTGACGG ACTGTCTAAT 
AGAATGCCAC ACAACCAATC GCAGGATTTC AGCACCACCA GCTCCCACAA CAGCTCAGAT 
CGGAGCAACA GTCTGTCAGG TGGAAAAGGT GGTAAGAAGA AGAAGACCAA AGCAAACGTC 
AAGCCCACCA AGATGAATGG CTCCAACTGG GCGAACGTTC CACTTCCTCC TCCTCCCATC 
CACCCTCTTC CTGGCACAGA GATGGACCAC TACGCCTCAG AGCACCATGA TGGAGGAGGA 
TATGATAGTG ATGGTTGGGG CCCACCATTG CCAGTGCAGA CGTACCTCCA TCAGGGTTTA 
GAGGATGAGC TGGAGGAAGA GGAAGAGCGG GTGCCAACTC CTCCCATTAG GGGTGTGGCT 
TCTTCTCCTG CTGCAGTTTC CTTTGGCAAA CAGTCCACAG CCACACTGAG CCCATCTCCA 
CGTGAAGAAA TGCAGCCCAT GCTCCAAGCC CACCTAGATG AGCTTACCCG TGCTTATCAA 
TTGGACATGG CTAAACAGAC TTGGCACATG CAGGGTGGGC CTTACCCGCC CCAGGCCCCC 
GCACCACCCG TGGGCTACGT CTCCAGCACC TTAGTGTCTG ACATCGAGAC CGATCTCCCT 
GATGAAGATG ACGATGATGA GGAGGATGAA GGATACGAGA TGTCGCCACC AATTAGGGGC 
ACTGAACACA CGCCAGGCTC CAGCATGGAC AACTTAGACA GCTCCGTTAC GGGCTCTATG 
GTGAACGGCT GGGGCTCGGC TTCAGAAGAT GATGACAGAA ACTTCTCCAG CAGGAGGTCT 
AGTGCCGGCA GCTCTTCTGA CGGCTCGCTC TTCACCCATT GCAGCTTTGC GCGAGCCCTG 
GTGGCAGCCG CAGACAAGGC TGGTTACCGT CTGGATGGCA CCAGCCTGAG CAAAAGAGAT 
AAAGGTCTAC CTCATAGACC TCGTCCCGCT AGCCCGTTCT CCACAGATGG AAGCACGGTG 
GGCACACACA GTCTGGGGCA CCAGCGGGCA GCAAGGCCCA CACGTAAACA CAAAGGCCCT 
GGCACTGTCC CGAACCGCCG TGATGCCAGT GCGGATGATC TGCCTCCACC TCCAGAGCCT 
CCACCCTGCC AGACAGCTCG CATCCCCGGG TCGAGGAACG TTCCCCATCC CTGCGACCGC 
AAAGCTTCCT CTCTGGAGAG GCAGCCTATG ATGGGCCTTG AGGAAATGAG AAGTTCCTTG 
GACCGGCAGG CCCGAACCTC ATTGGACCGC CACCAACAAG TCCAGGACAG ACTGGGGTCC 
ATAGAGAGAG GAGATGAAAG CAGAAGAGGA CAGAAAAATG GTCCCTTTTC AGAAGAGGCC 
CTATTGCCAT ACAACAAACC ATCATTCCCA TCCCCTGGTG GGCACAGCTC ATCTGGGACG 
GCCTCTTCTA AAGGGTCAAC GGGTCCTCGC AAAGGAGAAG GGACGAGGGG ACAGCACCAG 
CGCTCCACCA CGGAACAAGG AGAAAGCAGC TACATGGGGA CCAACGCGCA GTTTTCCGGA 
GAGTTATGTG AGTCTGAACA AGTTCAAGAG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

robo2 ( XM_017351002.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017206491.1 Danio rerio roundabout, axon guidance receptor, homolog 2 (Drosophila) (robo2), transcript variant X5, mRNA.

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CloneID	ODa55121
Clone ID Related Accession (Same CDS sequence)	XM_017351002.1
Accession Version	XM_017351002.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4752bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	roundabout homolog 2 isoform X5
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTCCTT TAACACACCT TTTACTCTGT GGATTAGTTT ACTTTAACCA AGTGGATGGA 
TCCCGTCTGC GGCAGGAAGA TTCTCCACCT CGGATTGTAG AACACCCCTC CGATCTGATC 
GTGTCAAAGG GCGAGCCAGC CACCCTAAAC TGCAAGGCGG AGGGTCGGCC AACTCCCACC 
GTGGAGTGGT ACAAGGATGG AGAACGAGTT GAAACGGACA AAGATGACCC TCGATCTCAC 
CGCATGCTCC TTCCCAGTGG TTCGCTCTTC TTCCTGCGTA TCGTACATGG ACGCCGCAGC 
AAACCTGATG AGGGGGCGTA CGTCTGTGTG GCACGCAACT ACTTGGGGGA AGCAGTCAGT 
CGAAACGCCT CGCTGGAAGT AGCATTGTTA CGGGATGATT TCCGGCAGAA CCCTACAGAT 
GTGGTGGTCG CTGCTGGAGA GCCGGCCATC CTGGAGTGTG TCCCTCCCAG AGGACACCCG 
GAGCCCACCA TCTACTGGAA GAAAGACAAA GTCCGAATTG ATGAGAAGGA TGACAGGATC 
AAGATCCGTG GAGGGAAGCT GATGATCTCT AACACCAGGA AAAGCGACGC TGGAATGTAC 
ATCTGCGTGG GCACCAACAT GGTCGGCGAA AGAGACAGTG AAACAGCCCA GGTCACTGTG 
TTCGAGAGGC CCACGTTCCT GCGGCGTCCC ATTAACCAGG TGGTTCTGGA GGAGGAGGCT 
GTGGAATTTC GATGTCAGGT GCAGGGAGAC CCGCAGCCGT CCATCCGCTG GAAAAAAGAC 
GACGTTGAAA TCCCCCGCGG CAGGTACGAT ATGAAATTTG ATAAAGATGA CTTCACGCTG 
AGAATAAAGA AGGCAACAGC GAGTGATGAA GGAACCTTCA CCTGTGTAGC GGAGAACCGA 
GTGGGCAAAA TGGAGGCCTC CGCAACCCTC ACGGTCAGAG CTCGCCCTGT CGCTGCACCG 
CAGTTCGTGA TCAGGCCGCG GGATCAGATT GTGGCACAGG GACGCACCGC CAGCTTCCCC 
TGCGAGACTA AAGGGAACCC GCAGCCGGCA GTCTTCTGGC AGAAAGAAGG AAGTCAGAAT 
CTCCTGTTTC CAAATCAACC CCAGCAGCCC AACAGTCGTT TCTCGGTGTC GCCCAGTGGA 
GATCTAACCA TCACTACGGT CCAGCGGGCA GATGCGGGCT ATTATATCTG CCAGGCCCTG 
ACGGTGGCGG GCAGCATCCT GGCCAAAGCA CAGCTTGAGG TCACAGACGT GTTGACCGAT 
CGCCCTCCAC CCATCATCCG GCAGGGTCCA GCTAACCAGA CTCTTGGTGT AGACAGTGTG 
GCTCTTTTGA GGTGCCATGC ATCCGGAGAA CCAGTCCCAA CCATCAGCTG GCTGAAGGAC 
GGGGTCAGTC TGCTGGGCAA AGACCCTCGG ATGTCCCTGC AAGACCTCGG CAGCCTGCAG 
ATCAAGGGTT TACGGCTCTC TGATTCTGGC ATTTATACTT GTGTGGCTGC AAGTTCTAGT 
GGGGAAACAT CATGGAGTGC TTTCTTGGAG GTCAAAGAGG CTGGGAGTCT TGTTGTAGTT 
AAAGATCGGG ATGAGAACGA GCTTCCAGGA CCTCCTTCCA AACCTCAGGT CACAGATGTC 
ACTAAAAACA GCGTGTCATT ATCCTGGCAG CCAGGCCTTC CCGGAGCATC AGCCATTTCT 
ACATATGTCA TAGAAGCATT CAGCCAATCG GTGAGCAACA GCTGGCAAAC TGTTGCCGAT 
CACGCCAAGA CAACGCAGTA CACCATTAAG GGTCTCCGCC CTAACACCAT CTACCTGTTC 
ATGGTGCGAG CGGTCAACGT CCAAGGCCTG AGCGACCCAA GCCCCATGTC TGAACCCGTC 
CGCACTCAAG ATATAAGTCC TCCAGCGCAG GGTGTGGATC ACAGACATGT GCAAAAGGAG 
CTGGGAGAAG TTATTGTGCG GCTACACAAC CCTGTGGTCC TTAGCCCTAC CACCATACAG 
GTCACATGGA CGGTTGACCG CCAGTCTCAG TTCATCCAGG GCTACAGGGT TTTGTACAGG 
CAGATGTCAG GCCTGTCTTC TCCAGGAGCC TGGCAAACTC AGGATGTGAA AGTCCCCTCA 
GAGAGGAGCA TGGTGCTCTC GGCTCTAAAG AAAGGCATCG TCTACGAGAT CAAAGTGCGT 
CCATATTTCA ATGAGTTTCA GGGCATGGAC AGCGAATCCC GAACCGCAAG AACAACAGAG 
GAAGCGCCCA GTGCTCCTCC TCAGCAGGTG ACTGTACTCA CTGTGGGCAA CCAGAACAGC 
ACATCTATTA GCATCTCCTG GGACCCTCCG CCTGCAGAAC ACCAGAACGG CATCATACAG 
GAGTACAAGA TCTGGTGTTT GGGGAATGAG ACCCGTTTTC ATGTGAATAA GACGGTGGAC 
GCAGCGATCC GCTCAGTGGT GGTTGGGGGT CTCCAGGCGG GGGTCCAGTA TCGGGTGGAG 
GTGGCCGCTA GCACCAGCGC CGGGGTCGGA GTGAAGAGCG AACCACAAAC CATCATCATT 
GGTAAAGACT TCCGCGATGT GATGATTAGC AGGAATAACA GCATCACAGA GCAGATCACG 
GATGTGGTGA AACAGCCGGC CTTCATCGCG GGCATTGGCG GGGCCTGCTG GGTCATTCTG 
ATGGGCTTCA GCATCTGGCT CTACTGGAGG AGGAAGAAGA GAAAGGGACT CAGCAATTAC 
GCAGTTCAGT CGTTCACCTT CACCCCTGCA GTGACGTTCC AGCGTGTTGA TGGAGGCTTG 
ATGAGCAACG GGAGTCGTCC TGGTCTGCTG AACGCCAGTG ATTCGGGTTA CCCGTGGCTC 
GCCGACTCCT GGCCTCCCAG CCTGCCTGTC AACAGCGGAT CTGGAGGACC CAATGACCTC 
AGCTTTGGCC GTGGAGATGT GATGCCCAGT GCTCAGGCTG ATAAGACGGG TACCATGCTT 
TCGGATGGAG CCATTTACAG CAGCATCGAC TTCACCACCA AAGGCGGATT CGGTAGCCCA 
AGCCCAGCCT CCCAGGCCAC GCCCTATGCC ACCACACAGA TCCTCCACTC CAACAGCATC 
CATGAGCTGG CAGTGGATCT TCCTGAAGCC CAGTGGAAGA CCTCCCTACA GGCCAAGCAG 
GAGATGGCCA GCCTGGGTTA CTCACTGCCC GACAAAAACT CCTGCAATAA CACACTCCTT 
TTCATTCCTG ACTACCGATT GGCTGACGGA CTGTCTAATA GAATGCCACA CAACCAATCG 
CAGGATTTCA GCACCACCAG CTCCCACAAC AGCTCAGATC GGAGCAACAG TCTGTCAGGT 
GGAAAAGGTG GTAAGAAGAA GAAGACCAAA GCAAACGTCA AGCCCACCAA GATGAATGGC 
TCCAACTGGG CGAACGTTCC ACTTCCTCCT CCTCCCATCC ACCCTCTTCC TGGCACAGAG 
ATGGACCACT ACGCCTCAGA GCACCATGAT GGAGGAGGAT ATGATAGTGA TGGTTGGGGC 
CCACCATTGC CAGTGCAGAC GTACCTCCAT CAGGGTTTAG AGGATGAGCT GGAGGAAGAG 
GAAGAGCGGG TGCCAACTCC TCCCATTAGG GGTGTGGCTT CTTCTCCTGC TGCAGTTTCC 
TTTGGCAAAC AGTCCACAGC CACACTGAGC CCATCTCCAC GTGAAGAAAT GCAGCCCATG 
CTCCAAGCCC ACCTAGATGA GCTTACCCGT GCTTATCAAT TGGACATGGC TAAACAGACT 
TGGCACATGC AGGGTGGGCC TTACCCGCCC CAGGCCCCCG CACCACCCGT GGGCTACGTC 
TCCAGCACCT TAGTGTCTGA CATCGAGACC GATCTCCCTG ATGAAGATGA CGATGATGAG 
GAGGATGAAG GATACGAGAT GTCGCCACCA ATTAGGGGCA CTGAACACAC GCCAGGCTCC 
AGCATGGACA ACTTAGACAG CTCCGTTACG GGCTCTATGG TGAACGGCTG GGGCTCGGCT 
TCAGAAGATG ATGACAGAAA CTTCTCCAGC AGGAGGTCTA GTGCCGGCAG CTCTTCTGAC 
GGCTCGCTCT TCACCCATTG CAGCTTTGCG CGAGCCCTGG TGGCAGCCGC AGACAAGGCT 
GGTTACCGTC TGGATGGCAC CAGCCTGAGC AAAAGAGATA AAGGTCTACC TCATAGACCT 
CGTCCCGCTA GCCCGTTCTC CACAGATGGA AGCACGGTGG GCACACACAG TCTGGGGCAC 
CAGCGGGCAG CAAGGCCCAC ACGTAAACAC AAAGGCCCTG GCACTGTCCC GAACCGCCGT 
GATGCCAGTG CGGATGATCT GCCTCCACCT CCAGAGCCTC CACCCTGCCA GACAGCTCGC 
ATCCCCGGGT CGAGGAACGT TCCCCATCCC TGCGACCGCA AAGCTTCCTC TCTGGAGAGG 
CAGCCTATGA TGGGCCTTGA GGAAATGAGA AGTTCCTTGG ACCGGCAGGC CCGAACCTCA 
TTGGACCGCC ACCAACAAGT CCAGGACAGA CTGGGGTCCA TAGAGAGAGG AGATGAAAGC 
AGAAGAGGAC AGAAAAATGG TCCCTTTTCA GAAGAGGCCC TATTGCCATA CAACAAACCA 
TCATTCCCAT CCCCTGGTGG GCACAGCTCA TCTGGGACGG CCTCTTCTAA AGGGTCAACG 
GGTCCTCGCA AAGGAGAAGG GACGAGGGGA CAGCACCAGC GCTCCACCAC GGAACAAGGA 
GAAAGCAGCT ACATGGGGAC CAACGCGCAG TTTTCCGGAG AGTTATGTGA GTCTGAACAA 
GTTCAAGAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017206491.1
CDS	616..5367
Translation	MGPLTHLLLCGLVYFNQVDGSRLRQEDSPPRIVEHPSDLIVSKGEPATLNCKAEGRPTPTVEWYKDGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRRSKPDEGAYVCVARNYLGEAVSRNASLEVALLRDDFRQNPTDVVVAAGEPAILECVPPRGHPEPTIYWKKDKVRIDEKDDRIKIRGGKLMISNTRKSDAGMYICVGTNMVGERDSETAQVTVFERPTFLRRPINQVVLEEEAVEFRCQVQGDPQPSIRWKKDDVEIPRGRYDMKFDKDDFTLRIKKATASDEGTFTCVAENRVGKMEASATLTVRARPVAAPQFVIRPRDQIVAQGRTASFPCETKGNPQPAVFWQKEGSQNLLFPNQPQQPNSRFSVSPSGDLTITTVQRADAGYYICQALTVAGSILAKAQLEVTDVLTDRPPPIIRQGPANQTLGVDSVALLRCHASGEPVPTISWLKDGVSLLGKDPRMSLQDLGSLQIKGLRLSDSGIYTCVAASSSGETSWSAFLEVKEAGSLVVVKDRDENELPGPPSKPQVTDVTKNSVSLSWQPGLPGASAISTYVIEAFSQSVSNSWQTVADHAKTTQYTIKGLRPNTIYLFMVRAVNVQGLSDPSPMSEPVRTQDISPPAQGVDHRHVQKELGEVIVRLHNPVVLSPTTIQVTWTVDRQSQFIQGYRVLYRQMSGLSSPGAWQTQDVKVPSERSMVLSALKKGIVYEIKVRPYFNEFQGMDSESRTARTTEEAPSAPPQQVTVLTVGNQNSTSISISWDPPPAEHQNGIIQEYKIWCLGNETRFHVNKTVDAAIRSVVVGGLQAGVQYRVEVAASTSAGVGVKSEPQTIIIGKDFRDVMISRNNSITEQITDVVKQPAFIAGIGGACWVILMGFSIWLYWRRKKRKGLSNYAVQSFTFTPAVTFQRVDGGLMSNGSRPGLLNASDSGYPWLADSWPPSLPVNSGSGGPNDLSFGRGDVMPSAQADKTGTMLSDGAIYSSIDFTTKGGFGSPSPASQATPYATTQILHSNSIHELAVDLPEAQWKTSLQAKQEMASLGYSLPDKNSCNNTLLFIPDYRLADGLSNRMPHNQSQDFSTTSSHNSSDRSNSLSGGKGGKKKKTKANVKPTKMNGSNWANVPLPPPPIHPLPGTEMDHYASEHHDGGGYDSDGWGPPLPVQTYLHQGLEDELEEEEERVPTPPIRGVASSPAAVSFGKQSTATLSPSPREEMQPMLQAHLDELTRAYQLDMAKQTWHMQGGPYPPQAPAPPVGYVSSTLVSDIETDLPDEDDDDEEDEGYEMSPPIRGTEHTPGSSMDNLDSSVTGSMVNGWGSASEDDDRNFSSRRSSAGSSSDGSLFTHCSFARALVAAADKAGYRLDGTSLSKRDKGLPHRPRPASPFSTDGSTVGTHSLGHQRAARPTRKHKGPGTVPNRRDASADDLPPPPEPPPCQTARIPGSRNVPHPCDRKASSLERQPMMGLEEMRSSLDRQARTSLDRHQQVQDRLGSIERGDESRRGQKNGPFSEEALLPYNKPSFPSPGGHSSSGTASSKGSTGPRKGEGTRGQHQRSTTEQGESSYMGTNAQFSGELCESEQVQE

Target ORF information:

RefSeq Version	XM_017351002.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio roundabout, axon guidance receptor, homolog 2 (Drosophila) (robo2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017351002.1

ORF Insert Sequence:

ATGGGTCCTT TAACACACCT TTTACTCTGT GGATTAGTTT ACTTTAACCA AGTGGATGGA 
TCCCGTCTGC GGCAGGAAGA TTCTCCACCT CGGATTGTAG AACACCCCTC CGATCTGATC 
GTGTCAAAGG GCGAGCCAGC CACCCTAAAC TGCAAGGCGG AGGGTCGGCC AACTCCCACC 
GTGGAGTGGT ACAAGGATGG AGAACGAGTT GAAACGGACA AAGATGACCC TCGATCTCAC 
CGCATGCTCC TTCCCAGTGG TTCGCTCTTC TTCCTGCGTA TCGTACATGG ACGCCGCAGC 
AAACCTGATG AGGGGGCGTA CGTCTGTGTG GCACGCAACT ACTTGGGGGA AGCAGTCAGT 
CGAAACGCCT CGCTGGAAGT AGCATTGTTA CGGGATGATT TCCGGCAGAA CCCTACAGAT 
GTGGTGGTCG CTGCTGGAGA GCCGGCCATC CTGGAGTGTG TCCCTCCCAG AGGACACCCG 
GAGCCCACCA TCTACTGGAA GAAAGACAAA GTCCGAATTG ATGAGAAGGA TGACAGGATC 
AAGATCCGTG GAGGGAAGCT GATGATCTCT AACACCAGGA AAAGCGACGC TGGAATGTAC 
ATCTGCGTGG GCACCAACAT GGTCGGCGAA AGAGACAGTG AAACAGCCCA GGTCACTGTG 
TTCGAGAGGC CCACGTTCCT GCGGCGTCCC ATTAACCAGG TGGTTCTGGA GGAGGAGGCT 
GTGGAATTTC GATGTCAGGT GCAGGGAGAC CCGCAGCCGT CCATCCGCTG GAAAAAAGAC 
GACGTTGAAA TCCCCCGCGG CAGGTACGAT ATGAAATTTG ATAAAGATGA CTTCACGCTG 
AGAATAAAGA AGGCAACAGC GAGTGATGAA GGAACCTTCA CCTGTGTAGC GGAGAACCGA 
GTGGGCAAAA TGGAGGCCTC CGCAACCCTC ACGGTCAGAG CTCGCCCTGT CGCTGCACCG 
CAGTTCGTGA TCAGGCCGCG GGATCAGATT GTGGCACAGG GACGCACCGC CAGCTTCCCC 
TGCGAGACTA AAGGGAACCC GCAGCCGGCA GTCTTCTGGC AGAAAGAAGG AAGTCAGAAT 
CTCCTGTTTC CAAATCAACC CCAGCAGCCC AACAGTCGTT TCTCGGTGTC GCCCAGTGGA 
GATCTAACCA TCACTACGGT CCAGCGGGCA GATGCGGGCT ATTATATCTG CCAGGCCCTG 
ACGGTGGCGG GCAGCATCCT GGCCAAAGCA CAGCTTGAGG TCACAGACGT GTTGACCGAT 
CGCCCTCCAC CCATCATCCG GCAGGGTCCA GCTAACCAGA CTCTTGGTGT AGACAGTGTG 
GCTCTTTTGA GGTGCCATGC ATCCGGAGAA CCAGTCCCAA CCATCAGCTG GCTGAAGGAC 
GGGGTCAGTC TGCTGGGCAA AGACCCTCGG ATGTCCCTGC AAGACCTCGG CAGCCTGCAG 
ATCAAGGGTT TACGGCTCTC TGATTCTGGC ATTTATACTT GTGTGGCTGC AAGTTCTAGT 
GGGGAAACAT CATGGAGTGC TTTCTTGGAG GTCAAAGAGG CTGGGAGTCT TGTTGTAGTT 
AAAGATCGGG ATGAGAACGA GCTTCCAGGA CCTCCTTCCA AACCTCAGGT CACAGATGTC 
ACTAAAAACA GCGTGTCATT ATCCTGGCAG CCAGGCCTTC CCGGAGCATC AGCCATTTCT 
ACATATGTCA TAGAAGCATT CAGCCAATCG GTGAGCAACA GCTGGCAAAC TGTTGCCGAT 
CACGCCAAGA CAACGCAGTA CACCATTAAG GGTCTCCGCC CTAACACCAT CTACCTGTTC 
ATGGTGCGAG CGGTCAACGT CCAAGGCCTG AGCGACCCAA GCCCCATGTC TGAACCCGTC 
CGCACTCAAG ATATAAGTCC TCCAGCGCAG GGTGTGGATC ACAGACATGT GCAAAAGGAG 
CTGGGAGAAG TTATTGTGCG GCTACACAAC CCTGTGGTCC TTAGCCCTAC CACCATACAG 
GTCACATGGA CGGTTGACCG CCAGTCTCAG TTCATCCAGG GCTACAGGGT TTTGTACAGG 
CAGATGTCAG GCCTGTCTTC TCCAGGAGCC TGGCAAACTC AGGATGTGAA AGTCCCCTCA 
GAGAGGAGCA TGGTGCTCTC GGCTCTAAAG AAAGGCATCG TCTACGAGAT CAAAGTGCGT 
CCATATTTCA ATGAGTTTCA GGGCATGGAC AGCGAATCCC GAACCGCAAG AACAACAGAG 
GAAGCGCCCA GTGCTCCTCC TCAGCAGGTG ACTGTACTCA CTGTGGGCAA CCAGAACAGC 
ACATCTATTA GCATCTCCTG GGACCCTCCG CCTGCAGAAC ACCAGAACGG CATCATACAG 
GAGTACAAGA TCTGGTGTTT GGGGAATGAG ACCCGTTTTC ATGTGAATAA GACGGTGGAC 
GCAGCGATCC GCTCAGTGGT GGTTGGGGGT CTCCAGGCGG GGGTCCAGTA TCGGGTGGAG 
GTGGCCGCTA GCACCAGCGC CGGGGTCGGA GTGAAGAGCG AACCACAAAC CATCATCATT 
GGTAAAGACT TCCGCGATGT GATGATTAGC AGGAATAACA GCATCACAGA GCAGATCACG 
GATGTGGTGA AACAGCCGGC CTTCATCGCG GGCATTGGCG GGGCCTGCTG GGTCATTCTG 
ATGGGCTTCA GCATCTGGCT CTACTGGAGG AGGAAGAAGA GAAAGGGACT CAGCAATTAC 
GCAGTTCAGT CGTTCACCTT CACCCCTGCA GTGACGTTCC AGCGTGTTGA TGGAGGCTTG 
ATGAGCAACG GGAGTCGTCC TGGTCTGCTG AACGCCAGTG ATTCGGGTTA CCCGTGGCTC 
GCCGACTCCT GGCCTCCCAG CCTGCCTGTC AACAGCGGAT CTGGAGGACC CAATGACCTC 
AGCTTTGGCC GTGGAGATGT GATGCCCAGT GCTCAGGCTG ATAAGACGGG TACCATGCTT 
TCGGATGGAG CCATTTACAG CAGCATCGAC TTCACCACCA AAGGCGGATT CGGTAGCCCA 
AGCCCAGCCT CCCAGGCCAC GCCCTATGCC ACCACACAGA TCCTCCACTC CAACAGCATC 
CATGAGCTGG CAGTGGATCT TCCTGAAGCC CAGTGGAAGA CCTCCCTACA GGCCAAGCAG 
GAGATGGCCA GCCTGGGTTA CTCACTGCCC GACAAAAACT CCTGCAATAA CACACTCCTT 
TTCATTCCTG ACTACCGATT GGCTGACGGA CTGTCTAATA GAATGCCACA CAACCAATCG 
CAGGATTTCA GCACCACCAG CTCCCACAAC AGCTCAGATC GGAGCAACAG TCTGTCAGGT 
GGAAAAGGTG GTAAGAAGAA GAAGACCAAA GCAAACGTCA AGCCCACCAA GATGAATGGC 
TCCAACTGGG CGAACGTTCC ACTTCCTCCT CCTCCCATCC ACCCTCTTCC TGGCACAGAG 
ATGGACCACT ACGCCTCAGA GCACCATGAT GGAGGAGGAT ATGATAGTGA TGGTTGGGGC 
CCACCATTGC CAGTGCAGAC GTACCTCCAT CAGGGTTTAG AGGATGAGCT GGAGGAAGAG 
GAAGAGCGGG TGCCAACTCC TCCCATTAGG GGTGTGGCTT CTTCTCCTGC TGCAGTTTCC 
TTTGGCAAAC AGTCCACAGC CACACTGAGC CCATCTCCAC GTGAAGAAAT GCAGCCCATG 
CTCCAAGCCC ACCTAGATGA GCTTACCCGT GCTTATCAAT TGGACATGGC TAAACAGACT 
TGGCACATGC AGGGTGGGCC TTACCCGCCC CAGGCCCCCG CACCACCCGT GGGCTACGTC 
TCCAGCACCT TAGTGTCTGA CATCGAGACC GATCTCCCTG ATGAAGATGA CGATGATGAG 
GAGGATGAAG GATACGAGAT GTCGCCACCA ATTAGGGGCA CTGAACACAC GCCAGGCTCC 
AGCATGGACA ACTTAGACAG CTCCGTTACG GGCTCTATGG TGAACGGCTG GGGCTCGGCT 
TCAGAAGATG ATGACAGAAA CTTCTCCAGC AGGAGGTCTA GTGCCGGCAG CTCTTCTGAC 
GGCTCGCTCT TCACCCATTG CAGCTTTGCG CGAGCCCTGG TGGCAGCCGC AGACAAGGCT 
GGTTACCGTC TGGATGGCAC CAGCCTGAGC AAAAGAGATA AAGGTCTACC TCATAGACCT 
CGTCCCGCTA GCCCGTTCTC CACAGATGGA AGCACGGTGG GCACACACAG TCTGGGGCAC 
CAGCGGGCAG CAAGGCCCAC ACGTAAACAC AAAGGCCCTG GCACTGTCCC GAACCGCCGT 
GATGCCAGTG CGGATGATCT GCCTCCACCT CCAGAGCCTC CACCCTGCCA GACAGCTCGC 
ATCCCCGGGT CGAGGAACGT TCCCCATCCC TGCGACCGCA AAGCTTCCTC TCTGGAGAGG 
CAGCCTATGA TGGGCCTTGA GGAAATGAGA AGTTCCTTGG ACCGGCAGGC CCGAACCTCA 
TTGGACCGCC ACCAACAAGT CCAGGACAGA CTGGGGTCCA TAGAGAGAGG AGATGAAAGC 
AGAAGAGGAC AGAAAAATGG TCCCTTTTCA GAAGAGGCCC TATTGCCATA CAACAAACCA 
TCATTCCCAT CCCCTGGTGG GCACAGCTCA TCTGGGACGG CCTCTTCTAA AGGGTCAACG 
GGTCCTCGCA AAGGAGAAGG GACGAGGGGA CAGCACCAGC GCTCCACCAC GGAACAAGGA 
GAAAGCAGCT ACATGGGGAC CAACGCGCAG TTTTCCGGAG AGTTATGTGA GTCTGAACAA 
GTTCAAGAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

robo2 ( XM_017351003.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017206492.1 Danio rerio roundabout, axon guidance receptor, homolog 2 (Drosophila) (robo2), transcript variant X6, mRNA.

Price & Availability↑

CloneID	ODa55122
Clone ID Related Accession (Same CDS sequence)	XM_017351003.1
Accession Version	XM_017351003.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4740bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	roundabout homolog 2 isoform X6
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTCCTT TAACACACCT TTTACTCTGT GGATTAGTTT ACTTTAACCA AGTGGATGGA 
TCCCGTCTGC GGCAGGAAGA TTCTCCACCT CGGATTGTAG AACACCCCTC CGATCTGATC 
GTGTCAAAGG GCGAGCCAGC CACCCTAAAC TGCAAGGCGG AGGGTCGGCC AACTCCCACC 
GTGGAGTGGT ACAAGGATGG AGAACGAGTT GAAACGGACA AAGATGACCC TCGATCTCAC 
CGCATGCTCC TTCCCAGTGG TTCGCTCTTC TTCCTGCGTA TCGTACATGG ACGCCGCAGC 
AAACCTGATG AGGGGGCGTA CGTCTGTGTG GCACGCAACT ACTTGGGGGA AGCAGTCAGT 
CGAAACGCCT CGCTGGAAGT AGCATTGTTA CGGGATGATT TCCGGCAGAA CCCTACAGAT 
GTGGTGGTCG CTGCTGGAGA GCCGGCCATC CTGGAGTGTG TCCCTCCCAG AGGACACCCG 
GAGCCCACCA TCTACTGGAA GAAAGACAAA GTCCGAATTG ATGAGAAGGA TGACAGGATC 
AAGATCCGTG GAGGGAAGCT GATGATCTCT AACACCAGGA AAAGCGACGC TGGAATGTAC 
ATCTGCGTGG GCACCAACAT GGTCGGCGAA AGAGACAGTG AAACAGCCCA GGTCACTGTG 
TTCGAGAGGC CCACGTTCCT GCGGCGTCCC ATTAACCAGG TGGTTCTGGA GGAGGAGGCT 
GTGGAATTTC GATGTCAGGT GCAGGGAGAC CCGCAGCCGT CCATCCGCTG GAAAAAAGAC 
GACGTTGAAA TCCCCCGCGG CAGGTACGAT ATGAAATTTG ATAAAGATGA CTTCACGCTG 
AGAATAAAGA AGGCAACAGC GAGTGATGAA GGAACCTTCA CCTGTGTAGC GGAGAACCGA 
GTGGGCAAAA TGGAGGCCTC CGCAACCCTC ACGGTCAGAG CTGCACCGCA GTTCGTGATC 
AGGCCGCGGG ATCAGATTGT GGCACAGGGA CGCACCGCCA GCTTCCCCTG CGAGACTAAA 
GGGAACCCGC AGCCGGCAGT CTTCTGGCAG AAAGAAGGAA GTCAGAATCT CCTGTTTCCA 
AATCAACCCC AGCAGCCCAA CAGTCGTTTC TCGGTGTCGC CCAGTGGAGA TCTAACCATC 
ACTACGGTCC AGCGGGCAGA TGCGGGCTAT TATATCTGCC AGGCCCTGAC GGTGGCGGGC 
AGCATCCTGG CCAAAGCACA GCTTGAGGTC ACAGACGTGT TGACCGATCG CCCTCCACCC 
ATCATCCGGC AGGGTCCAGC TAACCAGACT CTTGGTGTAG ACAGTGTGGC TCTTTTGAGG 
TGCCATGCAT CCGGAGAACC AGTCCCAACC ATCAGCTGGC TGAAGGACGG GGTCAGTCTG 
CTGGGCAAAG ACCCTCGGAT GTCCCTGCAA GACCTCGGCA GCCTGCAGAT CAAGGGTTTA 
CGGCTCTCTG ATTCTGGCAT TTATACTTGT GTGGCTGCAA GTTCTAGTGG GGAAACATCA 
TGGAGTGCTT TCTTGGAGGT CAAAGAGGCT GGGAGTCTTG TTGTAGTTAA AGATCGGGAT 
GAGAACGAGC TTCCAGGACC TCCTTCCAAA CCTCAGGTCA CAGATGTCAC TAAAAACAGC 
GTGTCATTAT CCTGGCAGCC AGGCCTTCCC GGAGCATCAG CCATTTCTAC ATATGTCATA 
GAAGCATTCA GCCAATCGGT GAGCAACAGC TGGCAAACTG TTGCCGATCA CGCCAAGACA 
ACGCAGTACA CCATTAAGGG TCTCCGCCCT AACACCATCT ACCTGTTCAT GGTGCGAGCG 
GTCAACGTCC AAGGCCTGAG CGACCCAAGC CCCATGTCTG AACCCGTCCG CACTCAAGAT 
ATAAGTCCTC CAGCGCAGGG TGTGGATCAC AGACATGTGC AAAAGGAGCT GGGAGAAGTT 
ATTGTGCGGC TACACAACCC TGTGGTCCTT AGCCCTACCA CCATACAGGT CACATGGACG 
GTTGACCGCC AGTCTCAGTT CATCCAGGGC TACAGGGTTT TGTACAGGCA GATGTCAGGC 
CTGTCTTCTC CAGGAGCCTG GCAAACTCAG GATGTGAAAG TCCCCTCAGA GAGGAGCATG 
GTGCTCTCGG CTCTAAAGAA AGGCATCGTC TACGAGATCA AAGTGCGTCC ATATTTCAAT 
GAGTTTCAGG GCATGGACAG CGAATCCCGA ACCGCAAGAA CAACAGAGGA AGCGCCCAGT 
GCTCCTCCTC AGCAGGTGAC TGTACTCACT GTGGGCAACC AGAACAGCAC ATCTATTAGC 
ATCTCCTGGG ACCCTCCGCC TGCAGAACAC CAGAACGGCA TCATACAGGA GTACAAGATC 
TGGTGTTTGG GGAATGAGAC CCGTTTTCAT GTGAATAAGA CGGTGGACGC AGCGATCCGC 
TCAGTGGTGG TTGGGGGTCT CCAGGCGGGG GTCCAGTATC GGGTGGAGGT GGCCGCTAGC 
ACCAGCGCCG GGGTCGGAGT GAAGAGCGAA CCACAAACCA TCATCATTGG TAAAGACTTC 
CGCGATGTGA TGATTAGCAG GAATAACAGC ATCACAGAGC AGATCACGGA TGTGGTGAAA 
CAGCCGGCCT TCATCGCGGG CATTGGCGGG GCCTGCTGGG TCATTCTGAT GGGCTTCAGC 
ATCTGGCTCT ACTGGAGGAG GAAGAAGAGA AAGGGACTCA GCAATTACGC AGTTCAGTCG 
TTCACCTTCA CCCCTGCAGT GACGTTCCAG CGTGTTGATG GAGGCTTGAT GAGCAACGGG 
AGTCGTCCTG GTCTGCTGAA CGCCAGTGAT TCGGGTTACC CGTGGCTCGC CGACTCCTGG 
CCTCCCAGCC TGCCTGTCAA CAGCGGATCT GGAGGACCCA ATGACCTCAG CTTTGGCCGT 
GGAGATGTGA TGCCCAGTGC TCAGGCTGAT AAGACGGGTA CCATGCTTTC GGATGGAGCC 
ATTTACAGCA GCATCGACTT CACCACCAAA GGCGGATTCG GTAGCCCAAG CCCAGCCTCC 
CAGGCCACGC CCTATGCCAC CACACAGATC CTCCACTCCA ACAGCATCCA TGAGCTGGCA 
GTGGATCTTC CTGAAGCCCA GTGGAAGACC TCCCTACAGG CCAAGCAGGA GATGGCCAGC 
CTGGGTTACT CACTGCCCGA CAAAAACTCC TGCAATAACA CACTCCTTTT CATTCCTGAC 
TACCGATTGG CTGACGGACT GTCTAATAGA ATGCCACACA ACCAATCGCA GGATTTCAGC 
ACCACCAGCT CCCACAACAG CTCAGATCGG AGCAACAGTC TGTCAGGTGG AAAAGGTGGT 
AAGAAGAAGA AGACCAAAGC AAACGTCAAG CCCACCAAGA TGAATGGCTC CAACTGGGCG 
AACGTTCCAC TTCCTCCTCC TCCCATCCAC CCTCTTCCTG GCACAGAGAT GGACCACTAC 
GCCTCAGAGC ACCATGATGG AGGAGGATAT GATAGTGATG GTTGGGGCCC ACCATTGCCA 
GTGCAGACGT ACCTCCATCA GGGTTTAGAG GATGAGCTGG AGGAAGAGGA AGAGCGGGTG 
CCAACTCCTC CCATTAGGGG TGTGGCTTCT TCTCCTGCTG CAGTTTCCTT TGGCAAACAG 
TCCACAGCCA CACTGAGCCC ATCTCCACGT GAAGAAATGC AGCCCATGCT CCAAGCCCAC 
CTAGATGAGC TTACCCGTGC TTATCAATTG GACATGGCTA AACAGACTTG GCACATGCAG 
GGTGGGCCTT ACCCGCCCCA GGCCCCCGCA CCACCCGTGG GCTACGTCTC CAGCACCTTA 
GTGTCTGACA TCGAGACCGA TCTCCCTGAT GAAGATGACG ATGATGAGGA GGATGAAGGA 
TACGAGATGT CGCCACCAAT TAGGGGCACT GAACACACGC CAGGCTCCAG CATGGACAAC 
TTAGACAGCT CCGTTACGGG CTCTATGGTG AACGGCTGGG GCTCGGCTTC AGAAGATGAT 
GACAGAAACT TCTCCAGCAG GAGGTCTAGT GCCGGCAGCT CTTCTGACGG CTCGCTCTTC 
ACCCATTGCA GCTTTGCGCG AGCCCTGGTG GCAGCCGCAG ACAAGGCTGG TTACCGTCTG 
GATGGCACCA GCCTGAGCAA AAGAGATAAA GGTCTACCTC ATAGACCTCG TCCCGCTAGC 
CCGTTCTCCA CAGATGGAAG CACGGTGGGC ACACACAGTC TGGGGCACCA GCGGGCAGCA 
AGGCCCACAC GTAAACACAA AGGCCCTGGC ACTGTCCCGA ACCGCCGTGA TGCCAGTGCG 
GATGATCTGC CTCCACCTCC AGAGCCTCCA CCCTGCCAGA CAGCTCGCAT CCCCGGGTCG 
AGGAACGTTC CCCATCCCTG CGACCGCAAA GCTTCCTCTC TGGAGAGGCA GCCTATGATG 
GGCCTTGAGG AAATGAGAAG TTCCTTGGAC CGGCAGGCCC GAACCTCATT GGACCGCCAC 
CAACAAGTCC AGGACAGACT GGGGTCCATA GAGAGAGGAG ATGAAAGCAG AAGAGGACAG 
AAAAATGGTC CCTTTTCAGA AGAGGCCCTA TTGCCATACA ACAAACCATC ATTCCCATCC 
CCTGGTGGGC ACAGCTCATC TGGGACGGCC TCTTCTAAAG GGTCAACGGG TCCTCGCAAA 
GGAGAAGGGA CGAGGGGACA GCACCAGCGC TCCACCACGG AACAAGGAGA AAGCAGCTAC 
ATGGGGACCA ACGCGCAGTT TTCCGGAGAG TTATGTGAGT CTGAACAAGT TCAAGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017206492.1
CDS	1..4740
Translation	MGPLTHLLLCGLVYFNQVDGSRLRQEDSPPRIVEHPSDLIVSKGEPATLNCKAEGRPTPTVEWYKDGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRRSKPDEGAYVCVARNYLGEAVSRNASLEVALLRDDFRQNPTDVVVAAGEPAILECVPPRGHPEPTIYWKKDKVRIDEKDDRIKIRGGKLMISNTRKSDAGMYICVGTNMVGERDSETAQVTVFERPTFLRRPINQVVLEEEAVEFRCQVQGDPQPSIRWKKDDVEIPRGRYDMKFDKDDFTLRIKKATASDEGTFTCVAENRVGKMEASATLTVRAAPQFVIRPRDQIVAQGRTASFPCETKGNPQPAVFWQKEGSQNLLFPNQPQQPNSRFSVSPSGDLTITTVQRADAGYYICQALTVAGSILAKAQLEVTDVLTDRPPPIIRQGPANQTLGVDSVALLRCHASGEPVPTISWLKDGVSLLGKDPRMSLQDLGSLQIKGLRLSDSGIYTCVAASSSGETSWSAFLEVKEAGSLVVVKDRDENELPGPPSKPQVTDVTKNSVSLSWQPGLPGASAISTYVIEAFSQSVSNSWQTVADHAKTTQYTIKGLRPNTIYLFMVRAVNVQGLSDPSPMSEPVRTQDISPPAQGVDHRHVQKELGEVIVRLHNPVVLSPTTIQVTWTVDRQSQFIQGYRVLYRQMSGLSSPGAWQTQDVKVPSERSMVLSALKKGIVYEIKVRPYFNEFQGMDSESRTARTTEEAPSAPPQQVTVLTVGNQNSTSISISWDPPPAEHQNGIIQEYKIWCLGNETRFHVNKTVDAAIRSVVVGGLQAGVQYRVEVAASTSAGVGVKSEPQTIIIGKDFRDVMISRNNSITEQITDVVKQPAFIAGIGGACWVILMGFSIWLYWRRKKRKGLSNYAVQSFTFTPAVTFQRVDGGLMSNGSRPGLLNASDSGYPWLADSWPPSLPVNSGSGGPNDLSFGRGDVMPSAQADKTGTMLSDGAIYSSIDFTTKGGFGSPSPASQATPYATTQILHSNSIHELAVDLPEAQWKTSLQAKQEMASLGYSLPDKNSCNNTLLFIPDYRLADGLSNRMPHNQSQDFSTTSSHNSSDRSNSLSGGKGGKKKKTKANVKPTKMNGSNWANVPLPPPPIHPLPGTEMDHYASEHHDGGGYDSDGWGPPLPVQTYLHQGLEDELEEEEERVPTPPIRGVASSPAAVSFGKQSTATLSPSPREEMQPMLQAHLDELTRAYQLDMAKQTWHMQGGPYPPQAPAPPVGYVSSTLVSDIETDLPDEDDDDEEDEGYEMSPPIRGTEHTPGSSMDNLDSSVTGSMVNGWGSASEDDDRNFSSRRSSAGSSSDGSLFTHCSFARALVAAADKAGYRLDGTSLSKRDKGLPHRPRPASPFSTDGSTVGTHSLGHQRAARPTRKHKGPGTVPNRRDASADDLPPPPEPPPCQTARIPGSRNVPHPCDRKASSLERQPMMGLEEMRSSLDRQARTSLDRHQQVQDRLGSIERGDESRRGQKNGPFSEEALLPYNKPSFPSPGGHSSSGTASSKGSTGPRKGEGTRGQHQRSTTEQGESSYMGTNAQFSGELCESEQVQE

Target ORF information:

RefSeq Version	XM_017351003.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio roundabout, axon guidance receptor, homolog 2 (Drosophila) (robo2), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017351003.1

ORF Insert Sequence:

ATGGGTCCTT TAACACACCT TTTACTCTGT GGATTAGTTT ACTTTAACCA AGTGGATGGA 
TCCCGTCTGC GGCAGGAAGA TTCTCCACCT CGGATTGTAG AACACCCCTC CGATCTGATC 
GTGTCAAAGG GCGAGCCAGC CACCCTAAAC TGCAAGGCGG AGGGTCGGCC AACTCCCACC 
GTGGAGTGGT ACAAGGATGG AGAACGAGTT GAAACGGACA AAGATGACCC TCGATCTCAC 
CGCATGCTCC TTCCCAGTGG TTCGCTCTTC TTCCTGCGTA TCGTACATGG ACGCCGCAGC 
AAACCTGATG AGGGGGCGTA CGTCTGTGTG GCACGCAACT ACTTGGGGGA AGCAGTCAGT 
CGAAACGCCT CGCTGGAAGT AGCATTGTTA CGGGATGATT TCCGGCAGAA CCCTACAGAT 
GTGGTGGTCG CTGCTGGAGA GCCGGCCATC CTGGAGTGTG TCCCTCCCAG AGGACACCCG 
GAGCCCACCA TCTACTGGAA GAAAGACAAA GTCCGAATTG ATGAGAAGGA TGACAGGATC 
AAGATCCGTG GAGGGAAGCT GATGATCTCT AACACCAGGA AAAGCGACGC TGGAATGTAC 
ATCTGCGTGG GCACCAACAT GGTCGGCGAA AGAGACAGTG AAACAGCCCA GGTCACTGTG 
TTCGAGAGGC CCACGTTCCT GCGGCGTCCC ATTAACCAGG TGGTTCTGGA GGAGGAGGCT 
GTGGAATTTC GATGTCAGGT GCAGGGAGAC CCGCAGCCGT CCATCCGCTG GAAAAAAGAC 
GACGTTGAAA TCCCCCGCGG CAGGTACGAT ATGAAATTTG ATAAAGATGA CTTCACGCTG 
AGAATAAAGA AGGCAACAGC GAGTGATGAA GGAACCTTCA CCTGTGTAGC GGAGAACCGA 
GTGGGCAAAA TGGAGGCCTC CGCAACCCTC ACGGTCAGAG CTGCACCGCA GTTCGTGATC 
AGGCCGCGGG ATCAGATTGT GGCACAGGGA CGCACCGCCA GCTTCCCCTG CGAGACTAAA 
GGGAACCCGC AGCCGGCAGT CTTCTGGCAG AAAGAAGGAA GTCAGAATCT CCTGTTTCCA 
AATCAACCCC AGCAGCCCAA CAGTCGTTTC TCGGTGTCGC CCAGTGGAGA TCTAACCATC 
ACTACGGTCC AGCGGGCAGA TGCGGGCTAT TATATCTGCC AGGCCCTGAC GGTGGCGGGC 
AGCATCCTGG CCAAAGCACA GCTTGAGGTC ACAGACGTGT TGACCGATCG CCCTCCACCC 
ATCATCCGGC AGGGTCCAGC TAACCAGACT CTTGGTGTAG ACAGTGTGGC TCTTTTGAGG 
TGCCATGCAT CCGGAGAACC AGTCCCAACC ATCAGCTGGC TGAAGGACGG GGTCAGTCTG 
CTGGGCAAAG ACCCTCGGAT GTCCCTGCAA GACCTCGGCA GCCTGCAGAT CAAGGGTTTA 
CGGCTCTCTG ATTCTGGCAT TTATACTTGT GTGGCTGCAA GTTCTAGTGG GGAAACATCA 
TGGAGTGCTT TCTTGGAGGT CAAAGAGGCT GGGAGTCTTG TTGTAGTTAA AGATCGGGAT 
GAGAACGAGC TTCCAGGACC TCCTTCCAAA CCTCAGGTCA CAGATGTCAC TAAAAACAGC 
GTGTCATTAT CCTGGCAGCC AGGCCTTCCC GGAGCATCAG CCATTTCTAC ATATGTCATA 
GAAGCATTCA GCCAATCGGT GAGCAACAGC TGGCAAACTG TTGCCGATCA CGCCAAGACA 
ACGCAGTACA CCATTAAGGG TCTCCGCCCT AACACCATCT ACCTGTTCAT GGTGCGAGCG 
GTCAACGTCC AAGGCCTGAG CGACCCAAGC CCCATGTCTG AACCCGTCCG CACTCAAGAT 
ATAAGTCCTC CAGCGCAGGG TGTGGATCAC AGACATGTGC AAAAGGAGCT GGGAGAAGTT 
ATTGTGCGGC TACACAACCC TGTGGTCCTT AGCCCTACCA CCATACAGGT CACATGGACG 
GTTGACCGCC AGTCTCAGTT CATCCAGGGC TACAGGGTTT TGTACAGGCA GATGTCAGGC 
CTGTCTTCTC CAGGAGCCTG GCAAACTCAG GATGTGAAAG TCCCCTCAGA GAGGAGCATG 
GTGCTCTCGG CTCTAAAGAA AGGCATCGTC TACGAGATCA AAGTGCGTCC ATATTTCAAT 
GAGTTTCAGG GCATGGACAG CGAATCCCGA ACCGCAAGAA CAACAGAGGA AGCGCCCAGT 
GCTCCTCCTC AGCAGGTGAC TGTACTCACT GTGGGCAACC AGAACAGCAC ATCTATTAGC 
ATCTCCTGGG ACCCTCCGCC TGCAGAACAC CAGAACGGCA TCATACAGGA GTACAAGATC 
TGGTGTTTGG GGAATGAGAC CCGTTTTCAT GTGAATAAGA CGGTGGACGC AGCGATCCGC 
TCAGTGGTGG TTGGGGGTCT CCAGGCGGGG GTCCAGTATC GGGTGGAGGT GGCCGCTAGC 
ACCAGCGCCG GGGTCGGAGT GAAGAGCGAA CCACAAACCA TCATCATTGG TAAAGACTTC 
CGCGATGTGA TGATTAGCAG GAATAACAGC ATCACAGAGC AGATCACGGA TGTGGTGAAA 
CAGCCGGCCT TCATCGCGGG CATTGGCGGG GCCTGCTGGG TCATTCTGAT GGGCTTCAGC 
ATCTGGCTCT ACTGGAGGAG GAAGAAGAGA AAGGGACTCA GCAATTACGC AGTTCAGTCG 
TTCACCTTCA CCCCTGCAGT GACGTTCCAG CGTGTTGATG GAGGCTTGAT GAGCAACGGG 
AGTCGTCCTG GTCTGCTGAA CGCCAGTGAT TCGGGTTACC CGTGGCTCGC CGACTCCTGG 
CCTCCCAGCC TGCCTGTCAA CAGCGGATCT GGAGGACCCA ATGACCTCAG CTTTGGCCGT 
GGAGATGTGA TGCCCAGTGC TCAGGCTGAT AAGACGGGTA CCATGCTTTC GGATGGAGCC 
ATTTACAGCA GCATCGACTT CACCACCAAA GGCGGATTCG GTAGCCCAAG CCCAGCCTCC 
CAGGCCACGC CCTATGCCAC CACACAGATC CTCCACTCCA ACAGCATCCA TGAGCTGGCA 
GTGGATCTTC CTGAAGCCCA GTGGAAGACC TCCCTACAGG CCAAGCAGGA GATGGCCAGC 
CTGGGTTACT CACTGCCCGA CAAAAACTCC TGCAATAACA CACTCCTTTT CATTCCTGAC 
TACCGATTGG CTGACGGACT GTCTAATAGA ATGCCACACA ACCAATCGCA GGATTTCAGC 
ACCACCAGCT CCCACAACAG CTCAGATCGG AGCAACAGTC TGTCAGGTGG AAAAGGTGGT 
AAGAAGAAGA AGACCAAAGC AAACGTCAAG CCCACCAAGA TGAATGGCTC CAACTGGGCG 
AACGTTCCAC TTCCTCCTCC TCCCATCCAC CCTCTTCCTG GCACAGAGAT GGACCACTAC 
GCCTCAGAGC ACCATGATGG AGGAGGATAT GATAGTGATG GTTGGGGCCC ACCATTGCCA 
GTGCAGACGT ACCTCCATCA GGGTTTAGAG GATGAGCTGG AGGAAGAGGA AGAGCGGGTG 
CCAACTCCTC CCATTAGGGG TGTGGCTTCT TCTCCTGCTG CAGTTTCCTT TGGCAAACAG 
TCCACAGCCA CACTGAGCCC ATCTCCACGT GAAGAAATGC AGCCCATGCT CCAAGCCCAC 
CTAGATGAGC TTACCCGTGC TTATCAATTG GACATGGCTA AACAGACTTG GCACATGCAG 
GGTGGGCCTT ACCCGCCCCA GGCCCCCGCA CCACCCGTGG GCTACGTCTC CAGCACCTTA 
GTGTCTGACA TCGAGACCGA TCTCCCTGAT GAAGATGACG ATGATGAGGA GGATGAAGGA 
TACGAGATGT CGCCACCAAT TAGGGGCACT GAACACACGC CAGGCTCCAG CATGGACAAC 
TTAGACAGCT CCGTTACGGG CTCTATGGTG AACGGCTGGG GCTCGGCTTC AGAAGATGAT 
GACAGAAACT TCTCCAGCAG GAGGTCTAGT GCCGGCAGCT CTTCTGACGG CTCGCTCTTC 
ACCCATTGCA GCTTTGCGCG AGCCCTGGTG GCAGCCGCAG ACAAGGCTGG TTACCGTCTG 
GATGGCACCA GCCTGAGCAA AAGAGATAAA GGTCTACCTC ATAGACCTCG TCCCGCTAGC 
CCGTTCTCCA CAGATGGAAG CACGGTGGGC ACACACAGTC TGGGGCACCA GCGGGCAGCA 
AGGCCCACAC GTAAACACAA AGGCCCTGGC ACTGTCCCGA ACCGCCGTGA TGCCAGTGCG 
GATGATCTGC CTCCACCTCC AGAGCCTCCA CCCTGCCAGA CAGCTCGCAT CCCCGGGTCG 
AGGAACGTTC CCCATCCCTG CGACCGCAAA GCTTCCTCTC TGGAGAGGCA GCCTATGATG 
GGCCTTGAGG AAATGAGAAG TTCCTTGGAC CGGCAGGCCC GAACCTCATT GGACCGCCAC 
CAACAAGTCC AGGACAGACT GGGGTCCATA GAGAGAGGAG ATGAAAGCAG AAGAGGACAG 
AAAAATGGTC CCTTTTCAGA AGAGGCCCTA TTGCCATACA ACAAACCATC ATTCCCATCC 
CCTGGTGGGC ACAGCTCATC TGGGACGGCC TCTTCTAAAG GGTCAACGGG TCCTCGCAAA 
GGAGAAGGGA CGAGGGGACA GCACCAGCGC TCCACCACGG AACAAGGAGA AAGCAGCTAC 
ATGGGGACCA ACGCGCAGTT TTCCGGAGAG TTATGTGAGT CTGAACAAGT TCAAGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

robo2 ( XM_017351004.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017206493.1 Danio rerio roundabout, axon guidance receptor, homolog 2 (Drosophila) (robo2), transcript variant X7, mRNA.

Price & Availability↑

CloneID	ODa55123
Clone ID Related Accession (Same CDS sequence)	XM_017351004.1
Accession Version	XM_017351004.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4674bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	roundabout homolog 2 isoform X7
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCCTCC TCTCTCCTGT GAGACTCACT TTATTTCTGT TGGTCTTCCT GGATGTTCCT 
CATCCGTCCC TTCAGGGCAG GAATAAGGGC AGATGGAACA AGGGGGGATC CCGTCTGCGG 
CAGGAAGATT CTCCACCTCG GATTGTAGAA CACCCCTCCG ATCTGATCGT GTCAAAGGGC 
GAGCCAGCCA CCCTAAACTG CAAGGCGGAG GGTCGGCCAA CTCCCACCGT GGAGTGGTAC 
AAGGATGGAG AACGAGTTGA AACGGACAAA GATGACCCTC GATCTCACCG CATGCTCCTT 
CCCAGTGGTT CGCTCTTCTT CCTGCGTATC GTACATGGAC GCCGCAGCAA ACCTGATGAG 
GGGGCGTACG TCTGTGTGGC ACGCAACTAC TTGGGGGAAG CAGTCAGTCG AAACGCCTCG 
CTGGAAGTAG CATTGTTACG GGATGATTTC CGGCAGAACC CTACAGATGT GGTGGTCGCT 
GCTGGAGAGC CGGCCATCCT GGAGTGTGTC CCTCCCAGAG GACACCCGGA GCCCACCATC 
TACTGGAAGA AAGACAAAGT CCGAATTGAT GAGAAGGATG ACAGGATCAA GATCCGTGGA 
GGGAAGCTGA TGATCTCTAA CACCAGGAAA AGCGACGCTG GAATGTACAT CTGCGTGGGC 
ACCAACATGG TCGGCGAAAG AGACAGTGAA ACAGCCCAGG TCACTGTGTT CGAGAGGCCC 
ACGTTCCTGC GGCGTCCCAT TAACCAGGTG GTTCTGGAGG AGGAGGCTGT GGAATTTCGA 
TGTCAGGTGC AGGGAGACCC GCAGCCGTCC ATCCGCTGGA AAAAAGACGA CGTTGAAATC 
CCCCGCGGCA GGTACGATAT GAAATTTGAT AAAGATGACT TCACGCTGAG AATAAAGAAG 
GCAACAGCGA GTGATGAAGG AACCTTCACC TGTGTAGCGG AGAACCGAGT GGGCAAAATG 
GAGGCCTCCG CAACCCTCAC GGTCAGAGCT CGCCCTGTCG CTGCACCGCA GTTCGTGATC 
AGGCCGCGGG ATCAGATTGT GGCACAGGGA CGCACCGCCA GCTTCCCCTG CGAGACTAAA 
GGGAACCCGC AGCCGGCAGT CTTCTGGCAG AAAGAAGGAA GTCAGAATCT CCTGTTTCCA 
AATCAACCCC AGCAGCCCAA CAGTCGTTTC TCGGTGTCGC CCAGTGGAGA TCTAACCATC 
ACTACGGTCC AGCGGGCAGA TGCGGGCTAT TATATCTGCC AGGCCCTGAC GGTGGCGGGC 
AGCATCCTGG CCAAAGCACA GCTTGAGGTC ACAGACGTGT TGACCGATCG CCCTCCACCC 
ATCATCCGGC AGGGTCCAGC TAACCAGACT CTTGGTGTAG ACAGTGTGGC TCTTTTGAGG 
TGCCATGCAT CCGGAGAACC AGTCCCAACC ATCAGCTGGC TGAAGGACGG GGTCAGTCTG 
CTGGGCAAAG ACCCTCGGAT GTCCCTGCAA GACCTCGGCA GCCTGCAGAT CAAGGGTTTA 
CGGCTCTCTG ATTCTGGCAT TTATACTTGT GTGGCTGCAA GTTCTAGTGG GGAAACATCA 
TGGAGTGCTT TCTTGGAGGT CAAAGAGGCT GGGAGTCTTG TTGTAGTTAA AGATCGGGAT 
GAGAACGAGC TTCCAGGACC TCCTTCCAAA CCTCAGGTCA CAGATGTCAC TAAAAACAGC 
GTGTCATTAT CCTGGCAGCC AGGCCTTCCC GGAGCATCAG CCATTTCTAC ATATGTCATA 
GAAGCATTCA GCCAATCGGT GAGCAACAGC TGGCAAACTG TTGCCGATCA CGCCAAGACA 
ACGCAGTACA CCATTAAGGG TCTCCGCCCT AACACCATCT ACCTGTTCAT GGTGCGAGCG 
GTCAACGTCC AAGGCCTGAG CGACCCAAGC CCCATGTCTG AACCCGTCCG CACTCAAGAT 
ATAAGTCCTC CAGCGCAGGG TGTGGATCAC AGACATGTGC AAAAGGAGCT GGGAGAAGTT 
ATTGTGCGGC TACACAACCC TGTGGTCCTT AGCCCTACCA CCATACAGGT CACATGGACG 
GTTGACCGCC AGTCTCAGTT CATCCAGGGC TACAGGGTTT TGTACAGGCA GATGTCAGGC 
CTGTCTTCTC CAGGAGCCTG GCAAACTCAG GATGTGAAAG TCCCCTCAGA GAGGAGCATG 
GTGCTCTCGG CTCTAAAGAA AGGCATCGTC TACGAGATCA AAGTGCGTCC ATATTTCAAT 
GAGTTTCAGG GCATGGACAG CGAATCCCGA ACCGCAAGAA CAACAGAGGA AGCGCCCAGT 
GCTCCTCCTC AGCAGGTGAC TGTACTCACT GTGGGCAACC AGAACAGCAC ATCTATTAGC 
ATCTCCTGGG ACCCTCCGCC TGCAGAACAC CAGAACGGCA TCATACAGGA GTACAAGATC 
TGGTGTTTGG GGAATGAGAC CCGTTTTCAT GTGAATAAGA CGGTGGACGC AGCGATCCGC 
TCAGTGGTGG TTGGGGGTCT CCAGGCGGGG GTCCAGTATC GGGTGGAGGT GGCCGCTAGC 
ACCAGCGCCG GGGTCGGAGT GAAGAGCGAA CCACAAACCA TCATCATTGG TAAAGACTTC 
CGCGATGTGA TGATTAGCAG GAATAACAGC ATCACAGAGC AGATCACGGA TGTGGTGAAA 
CAGCCGGCCT TCATCGCGGG CATTGGCGGG GCCTGCTGGG TCATTCTGAT GGGCTTCAGC 
ATCTGGCTCT ACTGGAGGAG GAAGAAGAGA AAGGGACTCA GCAATTACGC AGTTCAGTCG 
TTCACCTTCA CCCCTGCAGT GACGTTCCAG CGTGTTGATG GAGGCTTGAT GAGCAACGGG 
AGTCGTCCTG GTCTGCTGAA CGCCAGTGAT TCGGGTTACC CGTGGCTCGC CGACTCCTGG 
CCTCCCAGCC TGCCTGTCAA CAGCGGATCT GGAGGACCCA ATGACCTCAG CTTTGGCCGT 
GGAGATGTGA TGCCCAGTGC TCAGGCTGAT AAGACGGGTA CCATGCTTTC GGATGGAGCC 
ATTTACAGCA GCATCGACTT CACCACCAAA GGCGGATTCG GTAGCCCAAG CCCAGCCTCC 
CAGGCCACGC CCTATGCCAC CACACAGATC CTCCACTCCA ACAGCATCCA TGAGCTGGCA 
GTGGATCTTC CTGAAGCCCA GTGGAAGACC TCCCTACAGG CCAAGCAGGA GATGGCCAGC 
CTGGGTTACT CACTGCCCGA CAAAAACTCC TGCAATAACA GTGGAAAAGG TGGTAAGAAG 
AAGAAGACCA AAGCAAACGT CAAGCCCACC AAGATGAATG GCTCCAACTG GGCGAACGTT 
CCACTTCCTC CTCCTCCCAT CCACCCTCTT CCTGGCACAG AGATGGACCA CTACGCCTCA 
GAGCACCATG ATGGAGGAGG ATATGATAGT GATGGTTGGG GCCCACCATT GCCAGTGCAG 
ACGTACCTCC ATCAGGGTTT AGAGGATGAG CTGGAGGAAG AGGAAGAGCG GGTGCCAACT 
CCTCCCATTA GGGGTGTGGC TTCTTCTCCT GCTGCAGTTT CCTTTGGCAA ACAGTCCACA 
GCCACACTGA GCCCATCTCC ACGTGAAGAA ATGCAGCCCA TGCTCCAAGC CCACCTAGAT 
GAGCTTACCC GTGCTTATCA ATTGGACATG GCTAAACAGA CTTGGCACAT GCAGGGTGGG 
CCTTACCCGC CCCAGGCCCC CGCACCACCC GTGGGCTACG TCTCCAGCAC CTTAGTGTCT 
GACATCGAGA CCGATCTCCC TGATGAAGAT GACGATGATG AGGAGGATGA AGGATACGAG 
ATGTCGCCAC CAATTAGGGG CACTGAACAC ACGCCAGGCT CCAGCATGGA CAACTTAGAC 
AGCTCCGTTA CGGGCTCTAT GGTGAACGGC TGGGGCTCGG CTTCAGAAGA TGATGACAGA 
AACTTCTCCA GCAGGAGGTC TAGTGCCGGC AGCTCTTCTG ACGGCTCGCT CTTCACCCAT 
TGCAGCTTTG CGCGAGCCCT GGTGGCAGCC GCAGACAAGG CTGGTTACCG TCTGGATGGC 
ACCAGCCTGA GCAAAAGAGA TAAAGGTCTA CCTCATAGAC CTCGTCCCGC TAGCCCGTTC 
TCCACAGATG GAAGCACGGT GGGCACACAC AGTCTGGGGC ACCAGCGGGC AGCAAGGCCC 
ACACGTAAAC ACAAAGGCCC TGGCACTGTC CCGAACCGCC GTGATGCCAG TGCGGATGAT 
CTGCCTCCAC CTCCAGAGCC TCCACCCTGC CAGACAGCTC GCATCCCCGG GTCGAGGAAC 
GTTCCCCATC CCTGCGACCG CAAAGCTTCC TCTCTGGAGA GGCAGCCTAT GATGGGCCTT 
GAGGAAATGA GAAGTTCCTT GGACCGGCAG GCCCGAACCT CATTGGACCG CCACCAACAA 
GTCCAGGACA GACTGGGGTC CATAGAGAGA GGAGATGAAA GCAGAAGAGG ACAGAAAAAT 
GGTCCCTTTT CAGAAGAGGC CCTATTGCCA TACAACAAAC CATCATTCCC ATCCCCTGGT 
GGGCACAGCT CATCTGGGAC GGCCTCTTCT AAAGGGTCAA CGGGTCCTCG CAAAGGAGAA 
GGGACGAGGG GACAGCACCA GCGCTCCACC ACGGAACAAG GAGAAAGCAG CTACATGGGG 
ACCAACGCGC AGTTTTCCGG AGAGTTATGT GAGTCTGAAC AAGTTCAAGA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017206493.1
CDS	680..5353
Translation	MFLLSPVRLTLFLLVFLDVPHPSLQGRNKGRWNKGGSRLRQEDSPPRIVEHPSDLIVSKGEPATLNCKAEGRPTPTVEWYKDGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRRSKPDEGAYVCVARNYLGEAVSRNASLEVALLRDDFRQNPTDVVVAAGEPAILECVPPRGHPEPTIYWKKDKVRIDEKDDRIKIRGGKLMISNTRKSDAGMYICVGTNMVGERDSETAQVTVFERPTFLRRPINQVVLEEEAVEFRCQVQGDPQPSIRWKKDDVEIPRGRYDMKFDKDDFTLRIKKATASDEGTFTCVAENRVGKMEASATLTVRARPVAAPQFVIRPRDQIVAQGRTASFPCETKGNPQPAVFWQKEGSQNLLFPNQPQQPNSRFSVSPSGDLTITTVQRADAGYYICQALTVAGSILAKAQLEVTDVLTDRPPPIIRQGPANQTLGVDSVALLRCHASGEPVPTISWLKDGVSLLGKDPRMSLQDLGSLQIKGLRLSDSGIYTCVAASSSGETSWSAFLEVKEAGSLVVVKDRDENELPGPPSKPQVTDVTKNSVSLSWQPGLPGASAISTYVIEAFSQSVSNSWQTVADHAKTTQYTIKGLRPNTIYLFMVRAVNVQGLSDPSPMSEPVRTQDISPPAQGVDHRHVQKELGEVIVRLHNPVVLSPTTIQVTWTVDRQSQFIQGYRVLYRQMSGLSSPGAWQTQDVKVPSERSMVLSALKKGIVYEIKVRPYFNEFQGMDSESRTARTTEEAPSAPPQQVTVLTVGNQNSTSISISWDPPPAEHQNGIIQEYKIWCLGNETRFHVNKTVDAAIRSVVVGGLQAGVQYRVEVAASTSAGVGVKSEPQTIIIGKDFRDVMISRNNSITEQITDVVKQPAFIAGIGGACWVILMGFSIWLYWRRKKRKGLSNYAVQSFTFTPAVTFQRVDGGLMSNGSRPGLLNASDSGYPWLADSWPPSLPVNSGSGGPNDLSFGRGDVMPSAQADKTGTMLSDGAIYSSIDFTTKGGFGSPSPASQATPYATTQILHSNSIHELAVDLPEAQWKTSLQAKQEMASLGYSLPDKNSCNNSGKGGKKKKTKANVKPTKMNGSNWANVPLPPPPIHPLPGTEMDHYASEHHDGGGYDSDGWGPPLPVQTYLHQGLEDELEEEEERVPTPPIRGVASSPAAVSFGKQSTATLSPSPREEMQPMLQAHLDELTRAYQLDMAKQTWHMQGGPYPPQAPAPPVGYVSSTLVSDIETDLPDEDDDDEEDEGYEMSPPIRGTEHTPGSSMDNLDSSVTGSMVNGWGSASEDDDRNFSSRRSSAGSSSDGSLFTHCSFARALVAAADKAGYRLDGTSLSKRDKGLPHRPRPASPFSTDGSTVGTHSLGHQRAARPTRKHKGPGTVPNRRDASADDLPPPPEPPPCQTARIPGSRNVPHPCDRKASSLERQPMMGLEEMRSSLDRQARTSLDRHQQVQDRLGSIERGDESRRGQKNGPFSEEALLPYNKPSFPSPGGHSSSGTASSKGSTGPRKGEGTRGQHQRSTTEQGESSYMGTNAQFSGELCESEQVQE

Target ORF information:

RefSeq Version	XM_017351004.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio roundabout, axon guidance receptor, homolog 2 (Drosophila) (robo2), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017351004.1

ORF Insert Sequence:

ATGTTCCTCC TCTCTCCTGT GAGACTCACT TTATTTCTGT TGGTCTTCCT GGATGTTCCT 
CATCCGTCCC TTCAGGGCAG GAATAAGGGC AGATGGAACA AGGGGGGATC CCGTCTGCGG 
CAGGAAGATT CTCCACCTCG GATTGTAGAA CACCCCTCCG ATCTGATCGT GTCAAAGGGC 
GAGCCAGCCA CCCTAAACTG CAAGGCGGAG GGTCGGCCAA CTCCCACCGT GGAGTGGTAC 
AAGGATGGAG AACGAGTTGA AACGGACAAA GATGACCCTC GATCTCACCG CATGCTCCTT 
CCCAGTGGTT CGCTCTTCTT CCTGCGTATC GTACATGGAC GCCGCAGCAA ACCTGATGAG 
GGGGCGTACG TCTGTGTGGC ACGCAACTAC TTGGGGGAAG CAGTCAGTCG AAACGCCTCG 
CTGGAAGTAG CATTGTTACG GGATGATTTC CGGCAGAACC CTACAGATGT GGTGGTCGCT 
GCTGGAGAGC CGGCCATCCT GGAGTGTGTC CCTCCCAGAG GACACCCGGA GCCCACCATC 
TACTGGAAGA AAGACAAAGT CCGAATTGAT GAGAAGGATG ACAGGATCAA GATCCGTGGA 
GGGAAGCTGA TGATCTCTAA CACCAGGAAA AGCGACGCTG GAATGTACAT CTGCGTGGGC 
ACCAACATGG TCGGCGAAAG AGACAGTGAA ACAGCCCAGG TCACTGTGTT CGAGAGGCCC 
ACGTTCCTGC GGCGTCCCAT TAACCAGGTG GTTCTGGAGG AGGAGGCTGT GGAATTTCGA 
TGTCAGGTGC AGGGAGACCC GCAGCCGTCC ATCCGCTGGA AAAAAGACGA CGTTGAAATC 
CCCCGCGGCA GGTACGATAT GAAATTTGAT AAAGATGACT TCACGCTGAG AATAAAGAAG 
GCAACAGCGA GTGATGAAGG AACCTTCACC TGTGTAGCGG AGAACCGAGT GGGCAAAATG 
GAGGCCTCCG CAACCCTCAC GGTCAGAGCT CGCCCTGTCG CTGCACCGCA GTTCGTGATC 
AGGCCGCGGG ATCAGATTGT GGCACAGGGA CGCACCGCCA GCTTCCCCTG CGAGACTAAA 
GGGAACCCGC AGCCGGCAGT CTTCTGGCAG AAAGAAGGAA GTCAGAATCT CCTGTTTCCA 
AATCAACCCC AGCAGCCCAA CAGTCGTTTC TCGGTGTCGC CCAGTGGAGA TCTAACCATC 
ACTACGGTCC AGCGGGCAGA TGCGGGCTAT TATATCTGCC AGGCCCTGAC GGTGGCGGGC 
AGCATCCTGG CCAAAGCACA GCTTGAGGTC ACAGACGTGT TGACCGATCG CCCTCCACCC 
ATCATCCGGC AGGGTCCAGC TAACCAGACT CTTGGTGTAG ACAGTGTGGC TCTTTTGAGG 
TGCCATGCAT CCGGAGAACC AGTCCCAACC ATCAGCTGGC TGAAGGACGG GGTCAGTCTG 
CTGGGCAAAG ACCCTCGGAT GTCCCTGCAA GACCTCGGCA GCCTGCAGAT CAAGGGTTTA 
CGGCTCTCTG ATTCTGGCAT TTATACTTGT GTGGCTGCAA GTTCTAGTGG GGAAACATCA 
TGGAGTGCTT TCTTGGAGGT CAAAGAGGCT GGGAGTCTTG TTGTAGTTAA AGATCGGGAT 
GAGAACGAGC TTCCAGGACC TCCTTCCAAA CCTCAGGTCA CAGATGTCAC TAAAAACAGC 
GTGTCATTAT CCTGGCAGCC AGGCCTTCCC GGAGCATCAG CCATTTCTAC ATATGTCATA 
GAAGCATTCA GCCAATCGGT GAGCAACAGC TGGCAAACTG TTGCCGATCA CGCCAAGACA 
ACGCAGTACA CCATTAAGGG TCTCCGCCCT AACACCATCT ACCTGTTCAT GGTGCGAGCG 
GTCAACGTCC AAGGCCTGAG CGACCCAAGC CCCATGTCTG AACCCGTCCG CACTCAAGAT 
ATAAGTCCTC CAGCGCAGGG TGTGGATCAC AGACATGTGC AAAAGGAGCT GGGAGAAGTT 
ATTGTGCGGC TACACAACCC TGTGGTCCTT AGCCCTACCA CCATACAGGT CACATGGACG 
GTTGACCGCC AGTCTCAGTT CATCCAGGGC TACAGGGTTT TGTACAGGCA GATGTCAGGC 
CTGTCTTCTC CAGGAGCCTG GCAAACTCAG GATGTGAAAG TCCCCTCAGA GAGGAGCATG 
GTGCTCTCGG CTCTAAAGAA AGGCATCGTC TACGAGATCA AAGTGCGTCC ATATTTCAAT 
GAGTTTCAGG GCATGGACAG CGAATCCCGA ACCGCAAGAA CAACAGAGGA AGCGCCCAGT 
GCTCCTCCTC AGCAGGTGAC TGTACTCACT GTGGGCAACC AGAACAGCAC ATCTATTAGC 
ATCTCCTGGG ACCCTCCGCC TGCAGAACAC CAGAACGGCA TCATACAGGA GTACAAGATC 
TGGTGTTTGG GGAATGAGAC CCGTTTTCAT GTGAATAAGA CGGTGGACGC AGCGATCCGC 
TCAGTGGTGG TTGGGGGTCT CCAGGCGGGG GTCCAGTATC GGGTGGAGGT GGCCGCTAGC 
ACCAGCGCCG GGGTCGGAGT GAAGAGCGAA CCACAAACCA TCATCATTGG TAAAGACTTC 
CGCGATGTGA TGATTAGCAG GAATAACAGC ATCACAGAGC AGATCACGGA TGTGGTGAAA 
CAGCCGGCCT TCATCGCGGG CATTGGCGGG GCCTGCTGGG TCATTCTGAT GGGCTTCAGC 
ATCTGGCTCT ACTGGAGGAG GAAGAAGAGA AAGGGACTCA GCAATTACGC AGTTCAGTCG 
TTCACCTTCA CCCCTGCAGT GACGTTCCAG CGTGTTGATG GAGGCTTGAT GAGCAACGGG 
AGTCGTCCTG GTCTGCTGAA CGCCAGTGAT TCGGGTTACC CGTGGCTCGC CGACTCCTGG 
CCTCCCAGCC TGCCTGTCAA CAGCGGATCT GGAGGACCCA ATGACCTCAG CTTTGGCCGT 
GGAGATGTGA TGCCCAGTGC TCAGGCTGAT AAGACGGGTA CCATGCTTTC GGATGGAGCC 
ATTTACAGCA GCATCGACTT CACCACCAAA GGCGGATTCG GTAGCCCAAG CCCAGCCTCC 
CAGGCCACGC CCTATGCCAC CACACAGATC CTCCACTCCA ACAGCATCCA TGAGCTGGCA 
GTGGATCTTC CTGAAGCCCA GTGGAAGACC TCCCTACAGG CCAAGCAGGA GATGGCCAGC 
CTGGGTTACT CACTGCCCGA CAAAAACTCC TGCAATAACA GTGGAAAAGG TGGTAAGAAG 
AAGAAGACCA AAGCAAACGT CAAGCCCACC AAGATGAATG GCTCCAACTG GGCGAACGTT 
CCACTTCCTC CTCCTCCCAT CCACCCTCTT CCTGGCACAG AGATGGACCA CTACGCCTCA 
GAGCACCATG ATGGAGGAGG ATATGATAGT GATGGTTGGG GCCCACCATT GCCAGTGCAG 
ACGTACCTCC ATCAGGGTTT AGAGGATGAG CTGGAGGAAG AGGAAGAGCG GGTGCCAACT 
CCTCCCATTA GGGGTGTGGC TTCTTCTCCT GCTGCAGTTT CCTTTGGCAA ACAGTCCACA 
GCCACACTGA GCCCATCTCC ACGTGAAGAA ATGCAGCCCA TGCTCCAAGC CCACCTAGAT 
GAGCTTACCC GTGCTTATCA ATTGGACATG GCTAAACAGA CTTGGCACAT GCAGGGTGGG 
CCTTACCCGC CCCAGGCCCC CGCACCACCC GTGGGCTACG TCTCCAGCAC CTTAGTGTCT 
GACATCGAGA CCGATCTCCC TGATGAAGAT GACGATGATG AGGAGGATGA AGGATACGAG 
ATGTCGCCAC CAATTAGGGG CACTGAACAC ACGCCAGGCT CCAGCATGGA CAACTTAGAC 
AGCTCCGTTA CGGGCTCTAT GGTGAACGGC TGGGGCTCGG CTTCAGAAGA TGATGACAGA 
AACTTCTCCA GCAGGAGGTC TAGTGCCGGC AGCTCTTCTG ACGGCTCGCT CTTCACCCAT 
TGCAGCTTTG CGCGAGCCCT GGTGGCAGCC GCAGACAAGG CTGGTTACCG TCTGGATGGC 
ACCAGCCTGA GCAAAAGAGA TAAAGGTCTA CCTCATAGAC CTCGTCCCGC TAGCCCGTTC 
TCCACAGATG GAAGCACGGT GGGCACACAC AGTCTGGGGC ACCAGCGGGC AGCAAGGCCC 
ACACGTAAAC ACAAAGGCCC TGGCACTGTC CCGAACCGCC GTGATGCCAG TGCGGATGAT 
CTGCCTCCAC CTCCAGAGCC TCCACCCTGC CAGACAGCTC GCATCCCCGG GTCGAGGAAC 
GTTCCCCATC CCTGCGACCG CAAAGCTTCC TCTCTGGAGA GGCAGCCTAT GATGGGCCTT 
GAGGAAATGA GAAGTTCCTT GGACCGGCAG GCCCGAACCT CATTGGACCG CCACCAACAA 
GTCCAGGACA GACTGGGGTC CATAGAGAGA GGAGATGAAA GCAGAAGAGG ACAGAAAAAT 
GGTCCCTTTT CAGAAGAGGC CCTATTGCCA TACAACAAAC CATCATTCCC ATCCCCTGGT 
GGGCACAGCT CATCTGGGAC GGCCTCTTCT AAAGGGTCAA CGGGTCCTCG CAAAGGAGAA 
GGGACGAGGG GACAGCACCA GCGCTCCACC ACGGAACAAG GAGAAAGCAG CTACATGGGG 
ACCAACGCGC AGTTTTCCGG AGAGTTATGT GAGTCTGAAC AAGTTCAAGA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

robo2 ( XM_017351006.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017206495.1 Danio rerio roundabout, axon guidance receptor, homolog 2 (Drosophila) (robo2), transcript variant X8, mRNA.

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CloneID	ODa55124
Clone ID Related Accession (Same CDS sequence)	XM_017351006.1
Accession Version	XM_017351006.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4647bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	roundabout homolog 2 isoform X8
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCCTCC TCTCTCCTGT GAGACTCACT TTATTTCTGT TGGTCTTCCT GGATGTTCCT 
CATCCGTCCC TTCAGGGCAG GAATAAGGGC AGATGGAACA AGGGGGGATC CCGTCTGCGG 
CAGGAAGATT CTCCACCTCG GATTGTAGAA CACCCCTCCG ATCTGATCGT GTCAAAGGGC 
GAGCCAGCCA CCCTAAACTG CAAGGCGGAG GGTCGGCCAA CTCCCACCGT GGAGTGGTAC 
AAGGATGGAG AACGAGTTGA AACGGACAAA GATGACCCTC GATCTCACCG CATGCTCCTT 
CCCAGTGGTT CGCTCTTCTT CCTGCGTATC GTACATGGAC GCCGCAGCAA ACCTGATGAG 
GGGGCGTACG TCTGTGTGGC ACGCAACTAC TTGGGGGAAG CAGTCAGTCG AAACGCCTCG 
CTGGAAGTAG CATTGTTACG GGATGATTTC CGGCAGAACC CTACAGATGT GGTGGTCGCT 
GCTGGAGAGC CGGCCATCCT GGAGTGTGTC CCTCCCAGAG GACACCCGGA GCCCACCATC 
TACTGGAAGA AAGACAAAGT CCGAATTGAT GAGAAGGATG ACAGGATCAA GATCCGTGGA 
GGGAAGCTGA TGATCTCTAA CACCAGGAAA AGCGACGCTG GAATGTACAT CTGCGTGGGC 
ACCAACATGG TCGGCGAAAG AGACAGTGAA ACAGCCCAGG TCACTGTGTT CGAGAGGCCC 
ACGTTCCTGC GGCGTCCCAT TAACCAGGTG GTTCTGGAGG AGGAGGCTGT GGAATTTCGA 
TGTCAGGTGC AGGGAGACCC GCAGCCGTCC ATCCGCTGGA AAAAAGACGA CGTTGAAATC 
CCCCGCGGCA GGTACGATAT GAAATTTGAT AAAGATGACT TCACGCTGAG AATAAAGAAG 
GCAACAGCGA GTGATGAAGG AACCTTCACC TGTGTAGCGG AGAACCGAGT GGGCAAAATG 
GAGGCCTCCG CAACCCTCAC GGTCAGAGCT CGCCCTGTCG CTGCACCGCA GTTCGTGATC 
AGGCCGCGGG ATCAGATTGT GGCACAGGGA CGCACCGCCA GCTTCCCCTG CGAGACTAAA 
GGGAACCCGC AGCCGGCAGT CTTCTGGCAG AAAGAAGGAA GTCAGAATCT CCTGTTTCCA 
AATCAACCCC AGCAGCCCAA CAGTCGTTTC TCGGTGTCGC CCAGTGGAGA TCTAACCATC 
ACTACGGTCC AGCGGGCAGA TGCGGGCTAT TATATCTGCC AGGCCCTGAC GGTGGCGGGC 
AGCATCCTGG CCAAAGCACA GCTTGAGGTC ACAGACGTGT TGACCGATCG CCCTCCACCC 
ATCATCCGGC AGGGTCCAGC TAACCAGACT CTTGGTGTAG ACAGTGTGGC TCTTTTGAGG 
TGCCATGCAT CCGGAGAACC AGTCCCAACC ATCAGCTGGC TGAAGGACGG GGTCAGTCTG 
CTGGGCAAAG ACCCTCGGAT GTCCCTGCAA GACCTCGGCA GCCTGCAGAT CAAGGGTTTA 
CGGCTCTCTG ATTCTGGCAT TTATACTTGT GTGGCTGCAA GTTCTAGTGG GGAAACATCA 
TGGAGTGCTT TCTTGGAGGT CAAAGAGGCT GGGAGTCTTG TTGTAGTTAA AGATCGGGAT 
GAGAACGAGC TTCCAGGACC TCCTTCCAAA CCTCAGGTCA CAGATGTCAC TAAAAACAGC 
GTGTCATTAT CCTGGCAGCC AGGCCTTCCC GGAGCATCAG CCATTTCTAC ATATGTCATA 
GAAGCATTCA GCCAATCGGT GAGCAACAGC TGGCAAACTG TTGCCGATCA CGCCAAGACA 
ACGCAGTACA CCATTAAGGG TCTCCGCCCT AACACCATCT ACCTGTTCAT GGTGCGAGCG 
GTCAACGTCC AAGGCCTGAG CGACCCAAGC CCCATGTCTG AACCCGTCCG CACTCAAGAT 
ATAAGTCCTC CAGCGCAGGG TGTGGATCAC AGACATGTGC AAAAGGAGCT GGGAGAAGTT 
ATTGTGCGGC TACACAACCC TGTGGTCCTT AGCCCTACCA CCATACAGGT CACATGGACG 
GTTGACCGCC AGTCTCAGTT CATCCAGGGC TACAGGGTTT TGTACAGGCA GATGTCAGGC 
CTGTCTTCTC CAGGAGCCTG GCAAACTCAG GATGTGAAAG TCCCCTCAGA GAGGAGCATG 
GTGCTCTCGG CTCTAAAGAA AGGCATCGTC TACGAGATCA AAGTGCGTCC ATATTTCAAT 
GAGTTTCAGG GCATGGACAG CGAATCCCGA ACCGCAAGAA CAACAGAGGA AGCGCCCAGT 
GCTCCTCCTC AGCAGGTGAC TGTACTCACT GTGGGCAACC AGAACAGCAC ATCTATTAGC 
ATCTCCTGGG ACCCTCCGCC TGCAGAACAC CAGAACGGCA TCATACAGGA GTACAAGATC 
TGGTGTTTGG GGAATGAGAC CCGTTTTCAT GTGAATAAGA CGGTGGACGC AGCGATCCGC 
TCAGTGGTGG TTGGGGGTCT CCAGGCGGGG GTCCAGTATC GGGTGGAGGT GGCCGCTAGC 
ACCAGCGCCG GGGTCGGAGT GAAGAGCGAA CCACAAACCA TCATCATTGG TAAAGACTTC 
CGCGATGTGA TGATTAGCAG GAATAACAGC ATCACAGAGC AGATCACGGA TGTGGTGAAA 
CAGCCGGCCT TCATCGCGGG CATTGGCGGG GCCTGCTGGG TCATTCTGAT GGGCTTCAGC 
ATCTGGCTCT ACTGGAGGAG GAAGAAGAGA AAGGGACTCA GCAATTACGC AGTGACGTTC 
CAGCGTGTTG ATGGAGGCTT GATGAGCAAC GGGAGTCGTC CTGGTCTGCT GAACGCCAGT 
GATTCGGGTT ACCCGTGGCT CGCCGACTCC TGGCCTCCCA GCCTGCCTGT CAACAGCGGA 
TCTGGAGGAC CCAATGACCT CAGCTTTGGC CGTGGAGATG TGATGCCCAG TGCTCAGGCT 
GATAAGACGG GTACCATGCT TTCGGATGGA GCCATTTACA GCAGCATCGA CTTCACCACC 
AAAGGCGGAT TCGGTAGCCC AAGCCCAGCC TCCCAGGCCA CGCCCTATGC CACCACACAG 
ATCCTCCACT CCAACAGCAT CCATGAGCTG GCAGTGGATC TTCCTGAAGC CCAGTGGAAG 
ACCTCCCTAC AGGCCAAGCA GGAGATGGCC AGCCTGGGTT ACTCACTGCC CGACAAAAAC 
TCCTGCAATA ACAGTGGAAA AGGTGGTAAG AAGAAGAAGA CCAAAGCAAA CGTCAAGCCC 
ACCAAGATGA ATGGCTCCAA CTGGGCGAAC GTTCCACTTC CTCCTCCTCC CATCCACCCT 
CTTCCTGGCA CAGAGATGGA CCACTACGCC TCAGAGCACC ATGATGGAGG AGGATATGAT 
AGTGATGGTT GGGGCCCACC ATTGCCAGTG CAGACGTACC TCCATCAGGG TTTAGAGGAT 
GAGCTGGAGG AAGAGGAAGA GCGGGTGCCA ACTCCTCCCA TTAGGGGTGT GGCTTCTTCT 
CCTGCTGCAG TTTCCTTTGG CAAACAGTCC ACAGCCACAC TGAGCCCATC TCCACGTGAA 
GAAATGCAGC CCATGCTCCA AGCCCACCTA GATGAGCTTA CCCGTGCTTA TCAATTGGAC 
ATGGCTAAAC AGACTTGGCA CATGCAGGGT GGGCCTTACC CGCCCCAGGC CCCCGCACCA 
CCCGTGGGCT ACGTCTCCAG CACCTTAGTG TCTGACATCG AGACCGATCT CCCTGATGAA 
GATGACGATG ATGAGGAGGA TGAAGGATAC GAGATGTCGC CACCAATTAG GGGCACTGAA 
CACACGCCAG GCTCCAGCAT GGACAACTTA GACAGCTCCG TTACGGGCTC TATGGTGAAC 
GGCTGGGGCT CGGCTTCAGA AGATGATGAC AGAAACTTCT CCAGCAGGAG GTCTAGTGCC 
GGCAGCTCTT CTGACGGCTC GCTCTTCACC CATTGCAGCT TTGCGCGAGC CCTGGTGGCA 
GCCGCAGACA AGGCTGGTTA CCGTCTGGAT GGCACCAGCC TGAGCAAAAG AGATAAAGGT 
CTACCTCATA GACCTCGTCC CGCTAGCCCG TTCTCCACAG ATGGAAGCAC GGTGGGCACA 
CACAGTCTGG GGCACCAGCG GGCAGCAAGG CCCACACGTA AACACAAAGG CCCTGGCACT 
GTCCCGAACC GCCGTGATGC CAGTGCGGAT GATCTGCCTC CACCTCCAGA GCCTCCACCC 
TGCCAGACAG CTCGCATCCC CGGGTCGAGG AACGTTCCCC ATCCCTGCGA CCGCAAAGCT 
TCCTCTCTGG AGAGGCAGCC TATGATGGGC CTTGAGGAAA TGAGAAGTTC CTTGGACCGG 
CAGGCCCGAA CCTCATTGGA CCGCCACCAA CAAGTCCAGG ACAGACTGGG GTCCATAGAG 
AGAGGAGATG AAAGCAGAAG AGGACAGAAA AATGGTCCCT TTTCAGAAGA GGCCCTATTG 
CCATACAACA AACCATCATT CCCATCCCCT GGTGGGCACA GCTCATCTGG GACGGCCTCT 
TCTAAAGGGT CAACGGGTCC TCGCAAAGGA GAAGGGACGA GGGGACAGCA CCAGCGCTCC 
ACCACGGAAC AAGGAGAAAG CAGCTACATG GGGACCAACG CGCAGTTTTC CGGAGAGTTA 
TGTGAGTCTG AACAAGTTCA AGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017206495.1
CDS	680..5326
Translation	MFLLSPVRLTLFLLVFLDVPHPSLQGRNKGRWNKGGSRLRQEDSPPRIVEHPSDLIVSKGEPATLNCKAEGRPTPTVEWYKDGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRRSKPDEGAYVCVARNYLGEAVSRNASLEVALLRDDFRQNPTDVVVAAGEPAILECVPPRGHPEPTIYWKKDKVRIDEKDDRIKIRGGKLMISNTRKSDAGMYICVGTNMVGERDSETAQVTVFERPTFLRRPINQVVLEEEAVEFRCQVQGDPQPSIRWKKDDVEIPRGRYDMKFDKDDFTLRIKKATASDEGTFTCVAENRVGKMEASATLTVRARPVAAPQFVIRPRDQIVAQGRTASFPCETKGNPQPAVFWQKEGSQNLLFPNQPQQPNSRFSVSPSGDLTITTVQRADAGYYICQALTVAGSILAKAQLEVTDVLTDRPPPIIRQGPANQTLGVDSVALLRCHASGEPVPTISWLKDGVSLLGKDPRMSLQDLGSLQIKGLRLSDSGIYTCVAASSSGETSWSAFLEVKEAGSLVVVKDRDENELPGPPSKPQVTDVTKNSVSLSWQPGLPGASAISTYVIEAFSQSVSNSWQTVADHAKTTQYTIKGLRPNTIYLFMVRAVNVQGLSDPSPMSEPVRTQDISPPAQGVDHRHVQKELGEVIVRLHNPVVLSPTTIQVTWTVDRQSQFIQGYRVLYRQMSGLSSPGAWQTQDVKVPSERSMVLSALKKGIVYEIKVRPYFNEFQGMDSESRTARTTEEAPSAPPQQVTVLTVGNQNSTSISISWDPPPAEHQNGIIQEYKIWCLGNETRFHVNKTVDAAIRSVVVGGLQAGVQYRVEVAASTSAGVGVKSEPQTIIIGKDFRDVMISRNNSITEQITDVVKQPAFIAGIGGACWVILMGFSIWLYWRRKKRKGLSNYAVTFQRVDGGLMSNGSRPGLLNASDSGYPWLADSWPPSLPVNSGSGGPNDLSFGRGDVMPSAQADKTGTMLSDGAIYSSIDFTTKGGFGSPSPASQATPYATTQILHSNSIHELAVDLPEAQWKTSLQAKQEMASLGYSLPDKNSCNNSGKGGKKKKTKANVKPTKMNGSNWANVPLPPPPIHPLPGTEMDHYASEHHDGGGYDSDGWGPPLPVQTYLHQGLEDELEEEEERVPTPPIRGVASSPAAVSFGKQSTATLSPSPREEMQPMLQAHLDELTRAYQLDMAKQTWHMQGGPYPPQAPAPPVGYVSSTLVSDIETDLPDEDDDDEEDEGYEMSPPIRGTEHTPGSSMDNLDSSVTGSMVNGWGSASEDDDRNFSSRRSSAGSSSDGSLFTHCSFARALVAAADKAGYRLDGTSLSKRDKGLPHRPRPASPFSTDGSTVGTHSLGHQRAARPTRKHKGPGTVPNRRDASADDLPPPPEPPPCQTARIPGSRNVPHPCDRKASSLERQPMMGLEEMRSSLDRQARTSLDRHQQVQDRLGSIERGDESRRGQKNGPFSEEALLPYNKPSFPSPGGHSSSGTASSKGSTGPRKGEGTRGQHQRSTTEQGESSYMGTNAQFSGELCESEQVQE

Target ORF information:

RefSeq Version	XM_017351006.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio roundabout, axon guidance receptor, homolog 2 (Drosophila) (robo2), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017351006.1

ORF Insert Sequence:

ATGTTCCTCC TCTCTCCTGT GAGACTCACT TTATTTCTGT TGGTCTTCCT GGATGTTCCT 
CATCCGTCCC TTCAGGGCAG GAATAAGGGC AGATGGAACA AGGGGGGATC CCGTCTGCGG 
CAGGAAGATT CTCCACCTCG GATTGTAGAA CACCCCTCCG ATCTGATCGT GTCAAAGGGC 
GAGCCAGCCA CCCTAAACTG CAAGGCGGAG GGTCGGCCAA CTCCCACCGT GGAGTGGTAC 
AAGGATGGAG AACGAGTTGA AACGGACAAA GATGACCCTC GATCTCACCG CATGCTCCTT 
CCCAGTGGTT CGCTCTTCTT CCTGCGTATC GTACATGGAC GCCGCAGCAA ACCTGATGAG 
GGGGCGTACG TCTGTGTGGC ACGCAACTAC TTGGGGGAAG CAGTCAGTCG AAACGCCTCG 
CTGGAAGTAG CATTGTTACG GGATGATTTC CGGCAGAACC CTACAGATGT GGTGGTCGCT 
GCTGGAGAGC CGGCCATCCT GGAGTGTGTC CCTCCCAGAG GACACCCGGA GCCCACCATC 
TACTGGAAGA AAGACAAAGT CCGAATTGAT GAGAAGGATG ACAGGATCAA GATCCGTGGA 
GGGAAGCTGA TGATCTCTAA CACCAGGAAA AGCGACGCTG GAATGTACAT CTGCGTGGGC 
ACCAACATGG TCGGCGAAAG AGACAGTGAA ACAGCCCAGG TCACTGTGTT CGAGAGGCCC 
ACGTTCCTGC GGCGTCCCAT TAACCAGGTG GTTCTGGAGG AGGAGGCTGT GGAATTTCGA 
TGTCAGGTGC AGGGAGACCC GCAGCCGTCC ATCCGCTGGA AAAAAGACGA CGTTGAAATC 
CCCCGCGGCA GGTACGATAT GAAATTTGAT AAAGATGACT TCACGCTGAG AATAAAGAAG 
GCAACAGCGA GTGATGAAGG AACCTTCACC TGTGTAGCGG AGAACCGAGT GGGCAAAATG 
GAGGCCTCCG CAACCCTCAC GGTCAGAGCT CGCCCTGTCG CTGCACCGCA GTTCGTGATC 
AGGCCGCGGG ATCAGATTGT GGCACAGGGA CGCACCGCCA GCTTCCCCTG CGAGACTAAA 
GGGAACCCGC AGCCGGCAGT CTTCTGGCAG AAAGAAGGAA GTCAGAATCT CCTGTTTCCA 
AATCAACCCC AGCAGCCCAA CAGTCGTTTC TCGGTGTCGC CCAGTGGAGA TCTAACCATC 
ACTACGGTCC AGCGGGCAGA TGCGGGCTAT TATATCTGCC AGGCCCTGAC GGTGGCGGGC 
AGCATCCTGG CCAAAGCACA GCTTGAGGTC ACAGACGTGT TGACCGATCG CCCTCCACCC 
ATCATCCGGC AGGGTCCAGC TAACCAGACT CTTGGTGTAG ACAGTGTGGC TCTTTTGAGG 
TGCCATGCAT CCGGAGAACC AGTCCCAACC ATCAGCTGGC TGAAGGACGG GGTCAGTCTG 
CTGGGCAAAG ACCCTCGGAT GTCCCTGCAA GACCTCGGCA GCCTGCAGAT CAAGGGTTTA 
CGGCTCTCTG ATTCTGGCAT TTATACTTGT GTGGCTGCAA GTTCTAGTGG GGAAACATCA 
TGGAGTGCTT TCTTGGAGGT CAAAGAGGCT GGGAGTCTTG TTGTAGTTAA AGATCGGGAT 
GAGAACGAGC TTCCAGGACC TCCTTCCAAA CCTCAGGTCA CAGATGTCAC TAAAAACAGC 
GTGTCATTAT CCTGGCAGCC AGGCCTTCCC GGAGCATCAG CCATTTCTAC ATATGTCATA 
GAAGCATTCA GCCAATCGGT GAGCAACAGC TGGCAAACTG TTGCCGATCA CGCCAAGACA 
ACGCAGTACA CCATTAAGGG TCTCCGCCCT AACACCATCT ACCTGTTCAT GGTGCGAGCG 
GTCAACGTCC AAGGCCTGAG CGACCCAAGC CCCATGTCTG AACCCGTCCG CACTCAAGAT 
ATAAGTCCTC CAGCGCAGGG TGTGGATCAC AGACATGTGC AAAAGGAGCT GGGAGAAGTT 
ATTGTGCGGC TACACAACCC TGTGGTCCTT AGCCCTACCA CCATACAGGT CACATGGACG 
GTTGACCGCC AGTCTCAGTT CATCCAGGGC TACAGGGTTT TGTACAGGCA GATGTCAGGC 
CTGTCTTCTC CAGGAGCCTG GCAAACTCAG GATGTGAAAG TCCCCTCAGA GAGGAGCATG 
GTGCTCTCGG CTCTAAAGAA AGGCATCGTC TACGAGATCA AAGTGCGTCC ATATTTCAAT 
GAGTTTCAGG GCATGGACAG CGAATCCCGA ACCGCAAGAA CAACAGAGGA AGCGCCCAGT 
GCTCCTCCTC AGCAGGTGAC TGTACTCACT GTGGGCAACC AGAACAGCAC ATCTATTAGC 
ATCTCCTGGG ACCCTCCGCC TGCAGAACAC CAGAACGGCA TCATACAGGA GTACAAGATC 
TGGTGTTTGG GGAATGAGAC CCGTTTTCAT GTGAATAAGA CGGTGGACGC AGCGATCCGC 
TCAGTGGTGG TTGGGGGTCT CCAGGCGGGG GTCCAGTATC GGGTGGAGGT GGCCGCTAGC 
ACCAGCGCCG GGGTCGGAGT GAAGAGCGAA CCACAAACCA TCATCATTGG TAAAGACTTC 
CGCGATGTGA TGATTAGCAG GAATAACAGC ATCACAGAGC AGATCACGGA TGTGGTGAAA 
CAGCCGGCCT TCATCGCGGG CATTGGCGGG GCCTGCTGGG TCATTCTGAT GGGCTTCAGC 
ATCTGGCTCT ACTGGAGGAG GAAGAAGAGA AAGGGACTCA GCAATTACGC AGTGACGTTC 
CAGCGTGTTG ATGGAGGCTT GATGAGCAAC GGGAGTCGTC CTGGTCTGCT GAACGCCAGT 
GATTCGGGTT ACCCGTGGCT CGCCGACTCC TGGCCTCCCA GCCTGCCTGT CAACAGCGGA 
TCTGGAGGAC CCAATGACCT CAGCTTTGGC CGTGGAGATG TGATGCCCAG TGCTCAGGCT 
GATAAGACGG GTACCATGCT TTCGGATGGA GCCATTTACA GCAGCATCGA CTTCACCACC 
AAAGGCGGAT TCGGTAGCCC AAGCCCAGCC TCCCAGGCCA CGCCCTATGC CACCACACAG 
ATCCTCCACT CCAACAGCAT CCATGAGCTG GCAGTGGATC TTCCTGAAGC CCAGTGGAAG 
ACCTCCCTAC AGGCCAAGCA GGAGATGGCC AGCCTGGGTT ACTCACTGCC CGACAAAAAC 
TCCTGCAATA ACAGTGGAAA AGGTGGTAAG AAGAAGAAGA CCAAAGCAAA CGTCAAGCCC 
ACCAAGATGA ATGGCTCCAA CTGGGCGAAC GTTCCACTTC CTCCTCCTCC CATCCACCCT 
CTTCCTGGCA CAGAGATGGA CCACTACGCC TCAGAGCACC ATGATGGAGG AGGATATGAT 
AGTGATGGTT GGGGCCCACC ATTGCCAGTG CAGACGTACC TCCATCAGGG TTTAGAGGAT 
GAGCTGGAGG AAGAGGAAGA GCGGGTGCCA ACTCCTCCCA TTAGGGGTGT GGCTTCTTCT 
CCTGCTGCAG TTTCCTTTGG CAAACAGTCC ACAGCCACAC TGAGCCCATC TCCACGTGAA 
GAAATGCAGC CCATGCTCCA AGCCCACCTA GATGAGCTTA CCCGTGCTTA TCAATTGGAC 
ATGGCTAAAC AGACTTGGCA CATGCAGGGT GGGCCTTACC CGCCCCAGGC CCCCGCACCA 
CCCGTGGGCT ACGTCTCCAG CACCTTAGTG TCTGACATCG AGACCGATCT CCCTGATGAA 
GATGACGATG ATGAGGAGGA TGAAGGATAC GAGATGTCGC CACCAATTAG GGGCACTGAA 
CACACGCCAG GCTCCAGCAT GGACAACTTA GACAGCTCCG TTACGGGCTC TATGGTGAAC 
GGCTGGGGCT CGGCTTCAGA AGATGATGAC AGAAACTTCT CCAGCAGGAG GTCTAGTGCC 
GGCAGCTCTT CTGACGGCTC GCTCTTCACC CATTGCAGCT TTGCGCGAGC CCTGGTGGCA 
GCCGCAGACA AGGCTGGTTA CCGTCTGGAT GGCACCAGCC TGAGCAAAAG AGATAAAGGT 
CTACCTCATA GACCTCGTCC CGCTAGCCCG TTCTCCACAG ATGGAAGCAC GGTGGGCACA 
CACAGTCTGG GGCACCAGCG GGCAGCAAGG CCCACACGTA AACACAAAGG CCCTGGCACT 
GTCCCGAACC GCCGTGATGC CAGTGCGGAT GATCTGCCTC CACCTCCAGA GCCTCCACCC 
TGCCAGACAG CTCGCATCCC CGGGTCGAGG AACGTTCCCC ATCCCTGCGA CCGCAAAGCT 
TCCTCTCTGG AGAGGCAGCC TATGATGGGC CTTGAGGAAA TGAGAAGTTC CTTGGACCGG 
CAGGCCCGAA CCTCATTGGA CCGCCACCAA CAAGTCCAGG ACAGACTGGG GTCCATAGAG 
AGAGGAGATG AAAGCAGAAG AGGACAGAAA AATGGTCCCT TTTCAGAAGA GGCCCTATTG 
CCATACAACA AACCATCATT CCCATCCCCT GGTGGGCACA GCTCATCTGG GACGGCCTCT 
TCTAAAGGGT CAACGGGTCC TCGCAAAGGA GAAGGGACGA GGGGACAGCA CCAGCGCTCC 
ACCACGGAAC AAGGAGAAAG CAGCTACATG GGGACCAACG CGCAGTTTTC CGGAGAGTTA 
TGTGAGTCTG AACAAGTTCA AGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

robo2 ( XM_017351007.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017206496.1 Danio rerio roundabout, axon guidance receptor, homolog 2 (Drosophila) (robo2), transcript variant X9, mRNA.

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CloneID	ODa55125
Clone ID Related Accession (Same CDS sequence)	XM_017351007.1
Accession Version	XM_017351007.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4614bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	roundabout homolog 2 isoform X9
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCCTCC TCTCTCCTGT GAGACTCACT TTATTTCTGT TGGTCTTCCT GGATGTTCCT 
CATCCGTCCC TTCAGGGCAG GAATAAGGGC AGATGGAACA AGGGGGGATC CCGTCTGCGG 
CAGGAAGATT CTCCACCTCG GATTGTAGAA CACCCCTCCG ATCTGATCGT GTCAAAGGGC 
GAGCCAGCCA CCCTAAACTG CAAGGCGGAG GGTCGGCCAA CTCCCACCGT GGAGTGGTAC 
AAGGATGGAG AACGAGTTGA AACGGACAAA GATGACCCTC GATCTCACCG CATGCTCCTT 
CCCAGTGGTT CGCTCTTCTT CCTGCGTATC GTACATGGAC GCCGCAGCAA ACCTGATGAG 
GGGGCGTACG TCTGTGTGGC ACGCAACTAC TTGGGGGAAG CAGTCAGTCG AAACGCCTCG 
CTGGAAGTAG CATTGTTACG GGATGATTTC CGGCAGAACC CTACAGATGT GGTGGTCGCT 
GCTGGAGAGC CGGCCATCCT GGAGTGTGTC CCTCCCAGAG GACACCCGGA GCCCACCATC 
TACTGGAAGA AAGACAAAGT CCGAATTGAT GAGAAGGATG ACAGGATCAA GATCCGTGGA 
GGGAAGCTGA TGATCTCTAA CACCAGGAAA AGCGACGCTG GAATGTACAT CTGCGTGGGC 
ACCAACATGG TCGGCGAAAG AGACAGTGAA ACAGCCCAGG TCACTGTGTT CGAGAGGCCC 
ACGTTCCTGC GGCGTCCCAT TAACCAGGTG GTTCTGGAGG AGGAGGCTGT GGAATTTCGA 
TGTCAGGTGC AGGGAGACCC GCAGCCGTCC ATCCGCTGGA AAAAAGACGA CGTTGAAATC 
CCCCGCGGCA GGTACGATAT GAAATTTGAT AAAGATGACT TCACGCTGAG AATAAAGAAG 
GCAACAGCGA GTGATGAAGG AACCTTCACC TGTGTAGCGG AGAACCGAGT GGGCAAAATG 
GAGGCCTCCG CAACCCTCAC GGTCAGAGCT CGCCCTGTCG CTGCACCGCA GTTCGTGATC 
AGGCCGCGGG ATCAGATTGT GGCACAGGGA CGCACCGCCA GCTTCCCCTG CGAGACTAAA 
GGGAACCCGC AGCCGGCAGT CTTCTGGCAG AAAGAAGGAA GTCAGAATCT CCTGTTTCCA 
AATCAACCCC AGCAGCCCAA CAGTCGTTTC TCGGTGTCGC CCAGTGGAGA TCTAACCATC 
ACTACGGTCC AGCGGGCAGA TGCGGGCTAT TATATCTGCC AGGCCCTGAC GGTGGCGGGC 
AGCATCCTGG CCAAAGCACA GCTTGAGGTC ACAGACGTGT TGACCGATCG CCCTCCACCC 
ATCATCCGGC AGGGTCCAGC TAACCAGACT CTTGGTGTAG ACAGTGTGGC TCTTTTGAGG 
TGCCATGCAT CCGGAGAACC AGTCCCAACC ATCAGCTGGC TGAAGGACGG GGTCAGTCTG 
CTGGGCAAAG ACCCTCGGAT GTCCCTGCAA GACCTCGGCA GCCTGCAGAT CAAGGGTTTA 
CGGCTCTCTG ATTCTGGCAT TTATACTTGT GTGGCTGCAA GTTCTAGTGG GGAAACATCA 
TGGAGTGCTT TCTTGGAGGT CAAAGAGGCT GGGAGTCTTG TTGTAGTTAA AGATCGGGAT 
GAGAACGAGC TTCCAGGACC TCCTTCCAAA CCTCAGGTCA CAGATGTCAC TAAAAACAGC 
GTGTCATTAT CCTGGCAGCC AGGCCTTCCC GGAGCATCAG CCATTTCTAC ATATGTCATA 
GAAGCATTCA GCCAATCGGT GAGCAACAGC TGGCAAACTG TTGCCGATCA CGCCAAGACA 
ACGCAGTACA CCATTAAGGG TCTCCGCCCT AACACCATCT ACCTGTTCAT GGTGCGAGCG 
GTCAACGTCC AAGGCCTGAG CGACCCAAGC CCCATGTCTG AACCCGTCCG CACTCAAGAT 
ATAAGTCCTC CAGCGCAGGG TGTGGATCAC AGACATGTGC AAAAGGAGCT GGGAGAAGTT 
ATTGTGCGGC TACACAACCC TGTGGTCCTT AGCCCTACCA CCATACAGGT CACATGGACG 
GTTGACCGCC AGTCTCAGTT CATCCAGGGC TACAGGGTTT TGTACAGGCA GATGTCAGGC 
CTGTCTTCTC CAGGAGCCTG GCAAACTCAG GATGTGAAAG TCCCCTCAGA GAGGAGCATG 
GTGCTCTCGG CTCTAAAGAA AGGCATCGTC TACGAGATCA AAGTGCGTCC ATATTTCAAT 
GAGTTTCAGG GCATGGACAG CGAATCCCGA ACCGCAAGAA CAACAGAGGA AGCGCCCAGT 
GCTCCTCCTC AGCAGGTGAC TGTACTCACT GTGGGCAACC AGAACAGCAC ATCTATTAGC 
ATCTCCTGGG ACCCTCCGCC TGCAGAACAC CAGAACGGCA TCATACAGGA GTACAAGATC 
TGGTGTTTGG GGAATGAGAC CCGTTTTCAT GTGAATAAGA CGGTGGACGC AGCGATCCGC 
TCAGTGGTGG TTGGGGGTCT CCAGGCGGGG GTCCAGTATC GGGTGGAGGT GGCCGCTAGC 
ACCAGCGCCG GGGTCGGAGT GAAGAGCGAA CCACAAACCA TCATCATTGG TAAAGACTTC 
CGCGATGTGA TGATTAGCAG GAATAACAGC ATCACAGAGC AGATCACGGA TGTGGTGAAA 
CAGCCGGCCT TCATCGCGGG CATTGGCGGG GCCTGCTGGG TCATTCTGAT GGGCTTCAGC 
ATCTGGCTCT ACTGGAGGAG GAAGAAGAGA AAGGGACTCA GCAATTACGC AGTTCAGTCG 
TTCACCTTCA CCCCTGCAGT GACGTTCCAG CGTGTTGATG GAGGCTTGAT GAGCAACGGG 
AGTCGTCCTG GTCTGCTGAA CGCCAGTGAT TCGGGTTACC CGTGGCTCGC CGACTCCTGG 
CCTCCCAGCC TGCCTGTCAA CAGCGGATCT GGAGGACCCA ATGACCTCAG CTTTGGCCGT 
GGAGATGTGA TGCCCAGTGC TCAGGCTGAT AAGACGGGTA CCATGCTTTC GGATGGAGCC 
ATTTACAGCA GCATCGACTT CACCACCAAA GGCGGATTCG GTAGCCCAAG CCCAGCCTCC 
CAGGCCACGC CCTATGCCAC CACACAGATC CTCCACTCCA ACAGCATCCA TGAGCTGGCA 
GTGGATCTTC CTGAAGCCCA GTGGAAGACC TCCCTACAGG CCAAGCAGGA GATGGCCAGC 
CTGGGTTACT CACTGCCCGA CAAAAACTCC TGCAATAACA CACTCCTTTT CATTCCTGAC 
TACCGATTGG CTGACGGACT GTCTAATAGA ATGCCACACA ACCAATCGCA GGATTTCAGC 
ACCACCAGCT CCCACAACAG CTCAGATCGG AGCAACAGTC TGTCAGGTGG AAAAGGTGGT 
AAGAAGAAGA AGACCAAAGC AAACGTCAAG CCCACCAAGA TGAATGGCTC CAACTGGGCG 
AACGTTCCAC TTCCTCCTCC TCCCATCCAC CCTCTTCCTG GCACAGAGAT GGACCACTAC 
GCCTCAGAGC ACCATGATGG AGGAGGATAT GATAGTGATG GTTGGGGCCC ACCATTGCCA 
GTGCAGACGT ACCTCCATCA GGGTTTAGAG GATGAGCTGG AGGAAGAGGA AGAGCGGGTG 
CCAACTCCTC CCATTAGGGG TGTGGCTTCT TCTCCTGCTG CAGTTTCCTT TGGCAAACAG 
TCCACAGCCA CACTGAGCCC ATCTCCACGT GAAGAAATGC AGCCCATGCT CCAAGCCCAC 
CTAGATGAGC TTACCCGTGC TTATCAATTG GACATGGCTA AACAGACTTG GCACATGCAG 
GGTGGGCCTT ACCCGCCCCA GGCCCCCGCA CCACCCGTGG GCTACGTCTC CAGCACCTTA 
GTGTCTGACA TCGAGACCGA TCTCCCTGAT GAAGATGACG ATGATGAGGA GGATGAAGGA 
TACGAGATGT CGCCACCAAT TAGGGGCACT GAACACACGC CAGGCTCCAG CATGGACAAC 
TTAGACAGCT CCGTTACGGA TAAAGGTCTA CCTCATAGAC CTCGTCCCGC TAGCCCGTTC 
TCCACAGATG GAAGCACGGT GGGCACACAC AGTCTGGGGC ACCAGCGGGC AGCAAGGCCC 
ACACGTAAAC ACAAAGGCCC TGGCACTGTC CCGAACCGCC GTGATGCCAG TGCGGATGAT 
CTGCCTCCAC CTCCAGAGCC TCCACCCTGC CAGACAGCTC GCATCCCCGG GTCGAGGAAC 
GTTCCCCATC CCTGCGACCG CAAAGCTTCC TCTCTGGAGA GGCAGCCTAT GATGGGCCTT 
GAGGAAATGA GAAGTTCCTT GGACCGGCAG GCCCGAACCT CATTGGACCG CCACCAACAA 
GTCCAGGACA GACTGGGGTC CATAGAGAGA GGAGATGAAA GCAGAAGAGG ACAGAAAAAT 
GGTCCCTTTT CAGAAGAGGC CCTATTGCCA TACAACAAAC CATCATTCCC ATCCCCTGGT 
GGGCACAGCT CATCTGGGAC GGCCTCTTCT AAAGGGTCAA CGGGTCCTCG CAAAGGAGAA 
GGGACGAGGG GACAGCACCA GCGCTCCACC ACGGAACAAG GAGAAAGCAG CTACATGGGG 
ACCAACGCGC AGTTTTCCGG AGAGTTATGT GAGTCTGAAC AAGTTCAAGA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017206496.1
CDS	678..5291
Translation	MFLLSPVRLTLFLLVFLDVPHPSLQGRNKGRWNKGGSRLRQEDSPPRIVEHPSDLIVSKGEPATLNCKAEGRPTPTVEWYKDGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRRSKPDEGAYVCVARNYLGEAVSRNASLEVALLRDDFRQNPTDVVVAAGEPAILECVPPRGHPEPTIYWKKDKVRIDEKDDRIKIRGGKLMISNTRKSDAGMYICVGTNMVGERDSETAQVTVFERPTFLRRPINQVVLEEEAVEFRCQVQGDPQPSIRWKKDDVEIPRGRYDMKFDKDDFTLRIKKATASDEGTFTCVAENRVGKMEASATLTVRARPVAAPQFVIRPRDQIVAQGRTASFPCETKGNPQPAVFWQKEGSQNLLFPNQPQQPNSRFSVSPSGDLTITTVQRADAGYYICQALTVAGSILAKAQLEVTDVLTDRPPPIIRQGPANQTLGVDSVALLRCHASGEPVPTISWLKDGVSLLGKDPRMSLQDLGSLQIKGLRLSDSGIYTCVAASSSGETSWSAFLEVKEAGSLVVVKDRDENELPGPPSKPQVTDVTKNSVSLSWQPGLPGASAISTYVIEAFSQSVSNSWQTVADHAKTTQYTIKGLRPNTIYLFMVRAVNVQGLSDPSPMSEPVRTQDISPPAQGVDHRHVQKELGEVIVRLHNPVVLSPTTIQVTWTVDRQSQFIQGYRVLYRQMSGLSSPGAWQTQDVKVPSERSMVLSALKKGIVYEIKVRPYFNEFQGMDSESRTARTTEEAPSAPPQQVTVLTVGNQNSTSISISWDPPPAEHQNGIIQEYKIWCLGNETRFHVNKTVDAAIRSVVVGGLQAGVQYRVEVAASTSAGVGVKSEPQTIIIGKDFRDVMISRNNSITEQITDVVKQPAFIAGIGGACWVILMGFSIWLYWRRKKRKGLSNYAVQSFTFTPAVTFQRVDGGLMSNGSRPGLLNASDSGYPWLADSWPPSLPVNSGSGGPNDLSFGRGDVMPSAQADKTGTMLSDGAIYSSIDFTTKGGFGSPSPASQATPYATTQILHSNSIHELAVDLPEAQWKTSLQAKQEMASLGYSLPDKNSCNNTLLFIPDYRLADGLSNRMPHNQSQDFSTTSSHNSSDRSNSLSGGKGGKKKKTKANVKPTKMNGSNWANVPLPPPPIHPLPGTEMDHYASEHHDGGGYDSDGWGPPLPVQTYLHQGLEDELEEEEERVPTPPIRGVASSPAAVSFGKQSTATLSPSPREEMQPMLQAHLDELTRAYQLDMAKQTWHMQGGPYPPQAPAPPVGYVSSTLVSDIETDLPDEDDDDEEDEGYEMSPPIRGTEHTPGSSMDNLDSSVTDKGLPHRPRPASPFSTDGSTVGTHSLGHQRAARPTRKHKGPGTVPNRRDASADDLPPPPEPPPCQTARIPGSRNVPHPCDRKASSLERQPMMGLEEMRSSLDRQARTSLDRHQQVQDRLGSIERGDESRRGQKNGPFSEEALLPYNKPSFPSPGGHSSSGTASSKGSTGPRKGEGTRGQHQRSTTEQGESSYMGTNAQFSGELCESEQVQE

Target ORF information:

RefSeq Version	XM_017351007.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio roundabout, axon guidance receptor, homolog 2 (Drosophila) (robo2), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017351007.1

ORF Insert Sequence:

ATGTTCCTCC TCTCTCCTGT GAGACTCACT TTATTTCTGT TGGTCTTCCT GGATGTTCCT 
CATCCGTCCC TTCAGGGCAG GAATAAGGGC AGATGGAACA AGGGGGGATC CCGTCTGCGG 
CAGGAAGATT CTCCACCTCG GATTGTAGAA CACCCCTCCG ATCTGATCGT GTCAAAGGGC 
GAGCCAGCCA CCCTAAACTG CAAGGCGGAG GGTCGGCCAA CTCCCACCGT GGAGTGGTAC 
AAGGATGGAG AACGAGTTGA AACGGACAAA GATGACCCTC GATCTCACCG CATGCTCCTT 
CCCAGTGGTT CGCTCTTCTT CCTGCGTATC GTACATGGAC GCCGCAGCAA ACCTGATGAG 
GGGGCGTACG TCTGTGTGGC ACGCAACTAC TTGGGGGAAG CAGTCAGTCG AAACGCCTCG 
CTGGAAGTAG CATTGTTACG GGATGATTTC CGGCAGAACC CTACAGATGT GGTGGTCGCT 
GCTGGAGAGC CGGCCATCCT GGAGTGTGTC CCTCCCAGAG GACACCCGGA GCCCACCATC 
TACTGGAAGA AAGACAAAGT CCGAATTGAT GAGAAGGATG ACAGGATCAA GATCCGTGGA 
GGGAAGCTGA TGATCTCTAA CACCAGGAAA AGCGACGCTG GAATGTACAT CTGCGTGGGC 
ACCAACATGG TCGGCGAAAG AGACAGTGAA ACAGCCCAGG TCACTGTGTT CGAGAGGCCC 
ACGTTCCTGC GGCGTCCCAT TAACCAGGTG GTTCTGGAGG AGGAGGCTGT GGAATTTCGA 
TGTCAGGTGC AGGGAGACCC GCAGCCGTCC ATCCGCTGGA AAAAAGACGA CGTTGAAATC 
CCCCGCGGCA GGTACGATAT GAAATTTGAT AAAGATGACT TCACGCTGAG AATAAAGAAG 
GCAACAGCGA GTGATGAAGG AACCTTCACC TGTGTAGCGG AGAACCGAGT GGGCAAAATG 
GAGGCCTCCG CAACCCTCAC GGTCAGAGCT CGCCCTGTCG CTGCACCGCA GTTCGTGATC 
AGGCCGCGGG ATCAGATTGT GGCACAGGGA CGCACCGCCA GCTTCCCCTG CGAGACTAAA 
GGGAACCCGC AGCCGGCAGT CTTCTGGCAG AAAGAAGGAA GTCAGAATCT CCTGTTTCCA 
AATCAACCCC AGCAGCCCAA CAGTCGTTTC TCGGTGTCGC CCAGTGGAGA TCTAACCATC 
ACTACGGTCC AGCGGGCAGA TGCGGGCTAT TATATCTGCC AGGCCCTGAC GGTGGCGGGC 
AGCATCCTGG CCAAAGCACA GCTTGAGGTC ACAGACGTGT TGACCGATCG CCCTCCACCC 
ATCATCCGGC AGGGTCCAGC TAACCAGACT CTTGGTGTAG ACAGTGTGGC TCTTTTGAGG 
TGCCATGCAT CCGGAGAACC AGTCCCAACC ATCAGCTGGC TGAAGGACGG GGTCAGTCTG 
CTGGGCAAAG ACCCTCGGAT GTCCCTGCAA GACCTCGGCA GCCTGCAGAT CAAGGGTTTA 
CGGCTCTCTG ATTCTGGCAT TTATACTTGT GTGGCTGCAA GTTCTAGTGG GGAAACATCA 
TGGAGTGCTT TCTTGGAGGT CAAAGAGGCT GGGAGTCTTG TTGTAGTTAA AGATCGGGAT 
GAGAACGAGC TTCCAGGACC TCCTTCCAAA CCTCAGGTCA CAGATGTCAC TAAAAACAGC 
GTGTCATTAT CCTGGCAGCC AGGCCTTCCC GGAGCATCAG CCATTTCTAC ATATGTCATA 
GAAGCATTCA GCCAATCGGT GAGCAACAGC TGGCAAACTG TTGCCGATCA CGCCAAGACA 
ACGCAGTACA CCATTAAGGG TCTCCGCCCT AACACCATCT ACCTGTTCAT GGTGCGAGCG 
GTCAACGTCC AAGGCCTGAG CGACCCAAGC CCCATGTCTG AACCCGTCCG CACTCAAGAT 
ATAAGTCCTC CAGCGCAGGG TGTGGATCAC AGACATGTGC AAAAGGAGCT GGGAGAAGTT 
ATTGTGCGGC TACACAACCC TGTGGTCCTT AGCCCTACCA CCATACAGGT CACATGGACG 
GTTGACCGCC AGTCTCAGTT CATCCAGGGC TACAGGGTTT TGTACAGGCA GATGTCAGGC 
CTGTCTTCTC CAGGAGCCTG GCAAACTCAG GATGTGAAAG TCCCCTCAGA GAGGAGCATG 
GTGCTCTCGG CTCTAAAGAA AGGCATCGTC TACGAGATCA AAGTGCGTCC ATATTTCAAT 
GAGTTTCAGG GCATGGACAG CGAATCCCGA ACCGCAAGAA CAACAGAGGA AGCGCCCAGT 
GCTCCTCCTC AGCAGGTGAC TGTACTCACT GTGGGCAACC AGAACAGCAC ATCTATTAGC 
ATCTCCTGGG ACCCTCCGCC TGCAGAACAC CAGAACGGCA TCATACAGGA GTACAAGATC 
TGGTGTTTGG GGAATGAGAC CCGTTTTCAT GTGAATAAGA CGGTGGACGC AGCGATCCGC 
TCAGTGGTGG TTGGGGGTCT CCAGGCGGGG GTCCAGTATC GGGTGGAGGT GGCCGCTAGC 
ACCAGCGCCG GGGTCGGAGT GAAGAGCGAA CCACAAACCA TCATCATTGG TAAAGACTTC 
CGCGATGTGA TGATTAGCAG GAATAACAGC ATCACAGAGC AGATCACGGA TGTGGTGAAA 
CAGCCGGCCT TCATCGCGGG CATTGGCGGG GCCTGCTGGG TCATTCTGAT GGGCTTCAGC 
ATCTGGCTCT ACTGGAGGAG GAAGAAGAGA AAGGGACTCA GCAATTACGC AGTTCAGTCG 
TTCACCTTCA CCCCTGCAGT GACGTTCCAG CGTGTTGATG GAGGCTTGAT GAGCAACGGG 
AGTCGTCCTG GTCTGCTGAA CGCCAGTGAT TCGGGTTACC CGTGGCTCGC CGACTCCTGG 
CCTCCCAGCC TGCCTGTCAA CAGCGGATCT GGAGGACCCA ATGACCTCAG CTTTGGCCGT 
GGAGATGTGA TGCCCAGTGC TCAGGCTGAT AAGACGGGTA CCATGCTTTC GGATGGAGCC 
ATTTACAGCA GCATCGACTT CACCACCAAA GGCGGATTCG GTAGCCCAAG CCCAGCCTCC 
CAGGCCACGC CCTATGCCAC CACACAGATC CTCCACTCCA ACAGCATCCA TGAGCTGGCA 
GTGGATCTTC CTGAAGCCCA GTGGAAGACC TCCCTACAGG CCAAGCAGGA GATGGCCAGC 
CTGGGTTACT CACTGCCCGA CAAAAACTCC TGCAATAACA CACTCCTTTT CATTCCTGAC 
TACCGATTGG CTGACGGACT GTCTAATAGA ATGCCACACA ACCAATCGCA GGATTTCAGC 
ACCACCAGCT CCCACAACAG CTCAGATCGG AGCAACAGTC TGTCAGGTGG AAAAGGTGGT 
AAGAAGAAGA AGACCAAAGC AAACGTCAAG CCCACCAAGA TGAATGGCTC CAACTGGGCG 
AACGTTCCAC TTCCTCCTCC TCCCATCCAC CCTCTTCCTG GCACAGAGAT GGACCACTAC 
GCCTCAGAGC ACCATGATGG AGGAGGATAT GATAGTGATG GTTGGGGCCC ACCATTGCCA 
GTGCAGACGT ACCTCCATCA GGGTTTAGAG GATGAGCTGG AGGAAGAGGA AGAGCGGGTG 
CCAACTCCTC CCATTAGGGG TGTGGCTTCT TCTCCTGCTG CAGTTTCCTT TGGCAAACAG 
TCCACAGCCA CACTGAGCCC ATCTCCACGT GAAGAAATGC AGCCCATGCT CCAAGCCCAC 
CTAGATGAGC TTACCCGTGC TTATCAATTG GACATGGCTA AACAGACTTG GCACATGCAG 
GGTGGGCCTT ACCCGCCCCA GGCCCCCGCA CCACCCGTGG GCTACGTCTC CAGCACCTTA 
GTGTCTGACA TCGAGACCGA TCTCCCTGAT GAAGATGACG ATGATGAGGA GGATGAAGGA 
TACGAGATGT CGCCACCAAT TAGGGGCACT GAACACACGC CAGGCTCCAG CATGGACAAC 
TTAGACAGCT CCGTTACGGA TAAAGGTCTA CCTCATAGAC CTCGTCCCGC TAGCCCGTTC 
TCCACAGATG GAAGCACGGT GGGCACACAC AGTCTGGGGC ACCAGCGGGC AGCAAGGCCC 
ACACGTAAAC ACAAAGGCCC TGGCACTGTC CCGAACCGCC GTGATGCCAG TGCGGATGAT 
CTGCCTCCAC CTCCAGAGCC TCCACCCTGC CAGACAGCTC GCATCCCCGG GTCGAGGAAC 
GTTCCCCATC CCTGCGACCG CAAAGCTTCC TCTCTGGAGA GGCAGCCTAT GATGGGCCTT 
GAGGAAATGA GAAGTTCCTT GGACCGGCAG GCCCGAACCT CATTGGACCG CCACCAACAA 
GTCCAGGACA GACTGGGGTC CATAGAGAGA GGAGATGAAA GCAGAAGAGG ACAGAAAAAT 
GGTCCCTTTT CAGAAGAGGC CCTATTGCCA TACAACAAAC CATCATTCCC ATCCCCTGGT 
GGGCACAGCT CATCTGGGAC GGCCTCTTCT AAAGGGTCAA CGGGTCCTCG CAAAGGAGAA 
GGGACGAGGG GACAGCACCA GCGCTCCACC ACGGAACAAG GAGAAAGCAG CTACATGGGG 
ACCAACGCGC AGTTTTCCGG AGAGTTATGT GAGTCTGAAC AAGTTCAAGA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

robo2 ( XM_017351008.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017206497.1 Danio rerio roundabout, axon guidance receptor, homolog 2 (Drosophila) (robo2), transcript variant X10, mRNA.

Price & Availability↑

CloneID	ODa55126
Clone ID Related Accession (Same CDS sequence)	XM_017351008.1
Accession Version	XM_017351008.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4566bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	roundabout homolog 2 isoform X10
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTCCTT TAACACACCT TTTACTCTGT GGATTAGTTT ACTTTAACCA AGTGGATGGA 
TCCCGTCTGC GGCAGGAAGA TTCTCCACCT CGGATTGTAG AACACCCCTC CGATCTGATC 
GTGTCAAAGG GCGAGCCAGC CACCCTAAAC TGCAAGGCGG AGGGTCGGCC AACTCCCACC 
GTGGAGTGGT ACAAGGATGG AGAACGAGTT GAAACGGACA AAGATGACCC TCGATCTCAC 
CGCATGCTCC TTCCCAGTGG TTCGCTCTTC TTCCTGCGTA TCGTACATGG ACGCCGCAGC 
AAACCTGATG AGGGGGCGTA CGTCTGTGTG GCACGCAACT ACTTGGGGGA AGCAGTCAGT 
CGAAACGCCT CGCTGGAAGT AGCATTGTTA CGGGATGATT TCCGGCAGAA CCCTACAGAT 
GTGGTGGTCG CTGCTGGAGA GCCGGCCATC CTGGAGTGTG TCCCTCCCAG AGGACACCCG 
GAGCCCACCA TCTACTGGAA GAAAGACAAA GTCCGAATTG ATGAGAAGGA TGACAGGATC 
AAGATCCGTG GAGGGAAGCT GATGATCTCT AACACCAGGA AAAGCGACGC TGGAATGTAC 
ATCTGCGTGG GCACCAACAT GGTCGGCGAA AGAGACAGTG AAACAGCCCA GGTCACTGTG 
TTCGAGAGGC CCACGTTCCT GCGGCGTCCC ATTAACCAGG TGGTTCTGGA GGAGGAGGCT 
GTGGAATTTC GATGTCAGGT GCAGGGAGAC CCGCAGCCGT CCATCCGCTG GAAAAAAGAC 
GACGTTGAAA TCCCCCGCGG CAGGTACGAT ATGAAATTTG ATAAAGATGA CTTCACGCTG 
AGAATAAAGA AGGCAACAGC GAGTGATGAA GGAACCTTCA CCTGTGTAGC GGAGAACCGA 
GTGGGCAAAA TGGAGGCCTC CGCAACCCTC ACGGTCAGAG CTCGCCCTGT CGCTGCACCG 
CAGTTCGTGA TCAGGCCGCG GGATCAGATT GTGGCACAGG GACGCACCGC CAGCTTCCCC 
TGCGAGACTA AAGGGAACCC GCAGCCGGCA GTCTTCTGGC AGAAAGAAGG AAGTCAGAAT 
CTCCTGTTTC CAAATCAACC CCAGCAGCCC AACAGTCGTT TCTCGGTGTC GCCCAGTGGA 
GATCTAACCA TCACTACGGT CCAGCGGGCA GATGCGGGCT ATTATATCTG CCAGGCCCTG 
ACGGTGGCGG GCAGCATCCT GGCCAAAGCA CAGCTTGAGG TCACAGACGT GTTGACCGAT 
CGCCCTCCAC CCATCATCCG GCAGGGTCCA GCTAACCAGA CTCTTGGTGT AGACAGTGTG 
GCTCTTTTGA GGTGCCATGC ATCCGGAGAA CCAGTCCCAA CCATCAGCTG GCTGAAGGAC 
GGGGTCAGTC TGCTGGGCAA AGACCCTCGG ATGTCCCTGC AAGACCTCGG CAGCCTGCAG 
ATCAAGGGTT TACGGCTCTC TGATTCTGGC ATTTATACTT GTGTGGCTGC AAGTTCTAGT 
GGGGAAACAT CATGGAGTGC TTTCTTGGAG GTCAAAGAGG CTGGGAGTCT TGTTGTAGTT 
AAAGATCGGG ATGAGAACGA GCTTCCAGGA CCTCCTTCCA AACCTCAGGT CACAGATGTC 
ACTAAAAACA GCGTGTCATT ATCCTGGCAG CCAGGCCTTC CCGGAGCATC AGCCATTTCT 
ACATATGTCA TAGAAGCATT CAGCCAATCG GTGAGCAACA GCTGGCAAAC TGTTGCCGAT 
CACGCCAAGA CAACGCAGTA CACCATTAAG GGTCTCCGCC CTAACACCAT CTACCTGTTC 
ATGGTGCGAG CGGTCAACGT CCAAGGCCTG AGCGACCCAA GCCCCATGTC TGAACCCGTC 
CGCACTCAAG ATATAAGTCC TCCAGCGCAG GGTGTGGATC ACAGACATGT GCAAAAGGAG 
CTGGGAGAAG TTATTGTGCG GCTACACAAC CCTGTGGTCC TTAGCCCTAC CACCATACAG 
GTCACATGGA CGGTTGACCG CCAGTCTCAG TTCATCCAGG GCTACAGGGT TTTGTACAGG 
CAGATGTCAG GCCTGTCTTC TCCAGGAGCC TGGCAAACTC AGGATGTGAA AGTCCCCTCA 
GAGAGGAGCA TGGTGCTCTC GGCTCTAAAG AAAGGCATCG TCTACGAGAT CAAAGTGCGT 
CCATATTTCA ATGAGTTTCA GGGCATGGAC AGCGAATCCC GAACCGCAAG AACAACAGAG 
GAAGCGCCCA GTGCTCCTCC TCAGCAGGTG ACTGTACTCA CTGTGGGCAA CCAGAACAGC 
ACATCTATTA GCATCTCCTG GGACCCTCCG CCTGCAGAAC ACCAGAACGG CATCATACAG 
GAGTACAAGA TCTGGTGTTT GGGGAATGAG ACCCGTTTTC ATGTGAATAA GACGGTGGAC 
GCAGCGATCC GCTCAGTGGT GGTTGGGGGT CTCCAGGCGG GGGTCCAGTA TCGGGTGGAG 
GTGGCCGCTA GCACCAGCGC CGGGGTCGGA GTGAAGAGCG AACCACAAAC CATCATCATT 
GGTAAAGACT TCCGCGATGT GATGATTAGC AGGAATAACA GCATCACAGA GCAGATCACG 
GATGTGGTGA AACAGCCGGC CTTCATCGCG GGCATTGGCG GGGCCTGCTG GGTCATTCTG 
ATGGGCTTCA GCATCTGGCT CTACTGGAGG AGGAAGAAGA GAAAGGGACT CAGCAATTAC 
GCAGTTCAGT CGTTCACCTT CACCCCTGCA GTGACGTTCC AGCGTGTTGA TGGAGGCTTG 
ATGAGCAACG GGAGTCGTCC TGGTCTGCTG AACGCCAGTG ATTCGGGTTA CCCGTGGCTC 
GCCGACTCCT GGCCTCCCAG CCTGCCTGTC AACAGCGGAT CTGGAGGACC CAATGACCTC 
AGCTTTGGCC GTGGAGATGT GATGCCCAGT GCTCAGGCTG ATAAGACGGG TACCATGCTT 
TCGGATGGAG CCATTTACAG CAGCATCGAC TTCACCACCA AAGGCGGATT CGGTAGCCCA 
AGCCCAGCCT CCCAGGCCAC GCCCTATGCC ACCACACAGA TCCTCCACTC CAACAGCATC 
CATGAGCTGG CAGTGGATCT TCCTGAAGCC CAGTGGAAGA CCTCCCTACA GGCCAAGCAG 
GAGATGGCCA GCCTGGGTTA CTCACTGCCC GACAAAAACT CCTGCAATAA CACACTCCTT 
TTCATTCCTG ACTACCGATT GGCTGACGGA CTGTCTAATA GAATGCCACA CAACCAATCG 
CAGGATTTCA GCACCACCAG CTCCCACAAC AGCTCAGATC GGAGCAACAG TCTGTCAGGT 
GGAAAAGGTG GTAAGAAGAA GAAGACCAAA GCAAACGTCA AGCCCACCAA GATGAATGGC 
TCCAACTGGG CGAACGTTCC ACTTCCTCCT CCTCCCATCC ACCCTCTTCC TGGCACAGAG 
ATGGACCACT ACGCCTCAGA GCACCATGAT GGAGGAGGAT ATGATAGTGA TGGTTGGGGC 
CCACCATTGC CAGTGCAGAC GTACCTCCAT CAGGGTTTAG AGGATGAGCT GGAGGAAGAG 
GAAGAGCGGG TGCCAACTCC TCCCATTAGG GGTGTGGCTT CTTCTCCTGC TGCAGTTTCC 
TTTGGCAAAC AGTCCACAGC CACACTGAGC CCATCTCCAC GTGAAGAAAT GCAGCCCATG 
CTCCAAGCCC ACCTAGATGA GCTTACCCGT GCTTATCAAT TGGACATGGC TAAACAGACT 
TGGCACATGC AGGGTGGGCC TTACCCGCCC CAGGCCCCCG CACCACCCGT GGGCTACGTC 
TCCAGCACCT TAGTGTCTGA CATCGAGACC GATCTCCCTG ATGAAGATGA CGATGATGAG 
GAGGATGAAG GATACGAGAT GTCGCCACCA ATTAGGGGCA CTGAACACAC GCCAGGCTCC 
AGCATGGACA ACTTAGACAG CTCCGTTACG GATAAAGGTC TACCTCATAG ACCTCGTCCC 
GCTAGCCCGT TCTCCACAGA TGGAAGCACG GTGGGCACAC ACAGTCTGGG GCACCAGCGG 
GCAGCAAGGC CCACACGTAA ACACAAAGGC CCTGGCACTG TCCCGAACCG CCGTGATGCC 
AGTGCGGATG ATCTGCCTCC ACCTCCAGAG CCTCCACCCT GCCAGACAGC TCGCATCCCC 
GGGTCGAGGA ACGTTCCCCA TCCCTGCGAC CGCAAAGCTT CCTCTCTGGA GAGGCAGCCT 
ATGATGGGCC TTGAGGAAAT GAGAAGTTCC TTGGACCGGC AGGCCCGAAC CTCATTGGAC 
CGCCACCAAC AAGTCCAGGA CAGACTGGGG TCCATAGAGA GAGGAGATGA AAGCAGAAGA 
GGACAGAAAA ATGGTCCCTT TTCAGAAGAG GCCCTATTGC CATACAACAA ACCATCATTC 
CCATCCCCTG GTGGGCACAG CTCATCTGGG ACGGCCTCTT CTAAAGGGTC AACGGGTCCT 
CGCAAAGGAG AAGGGACGAG GGGACAGCAC CAGCGCTCCA CCACGGAACA AGGAGAAAGC 
AGCTACATGG GGACCAACGC GCAGTTTTCC GGAGAGTTAT GTGAGTCTGA ACAAGTTCAA 
GAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017206497.1
CDS	1..4566
Translation	MGPLTHLLLCGLVYFNQVDGSRLRQEDSPPRIVEHPSDLIVSKGEPATLNCKAEGRPTPTVEWYKDGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRRSKPDEGAYVCVARNYLGEAVSRNASLEVALLRDDFRQNPTDVVVAAGEPAILECVPPRGHPEPTIYWKKDKVRIDEKDDRIKIRGGKLMISNTRKSDAGMYICVGTNMVGERDSETAQVTVFERPTFLRRPINQVVLEEEAVEFRCQVQGDPQPSIRWKKDDVEIPRGRYDMKFDKDDFTLRIKKATASDEGTFTCVAENRVGKMEASATLTVRARPVAAPQFVIRPRDQIVAQGRTASFPCETKGNPQPAVFWQKEGSQNLLFPNQPQQPNSRFSVSPSGDLTITTVQRADAGYYICQALTVAGSILAKAQLEVTDVLTDRPPPIIRQGPANQTLGVDSVALLRCHASGEPVPTISWLKDGVSLLGKDPRMSLQDLGSLQIKGLRLSDSGIYTCVAASSSGETSWSAFLEVKEAGSLVVVKDRDENELPGPPSKPQVTDVTKNSVSLSWQPGLPGASAISTYVIEAFSQSVSNSWQTVADHAKTTQYTIKGLRPNTIYLFMVRAVNVQGLSDPSPMSEPVRTQDISPPAQGVDHRHVQKELGEVIVRLHNPVVLSPTTIQVTWTVDRQSQFIQGYRVLYRQMSGLSSPGAWQTQDVKVPSERSMVLSALKKGIVYEIKVRPYFNEFQGMDSESRTARTTEEAPSAPPQQVTVLTVGNQNSTSISISWDPPPAEHQNGIIQEYKIWCLGNETRFHVNKTVDAAIRSVVVGGLQAGVQYRVEVAASTSAGVGVKSEPQTIIIGKDFRDVMISRNNSITEQITDVVKQPAFIAGIGGACWVILMGFSIWLYWRRKKRKGLSNYAVQSFTFTPAVTFQRVDGGLMSNGSRPGLLNASDSGYPWLADSWPPSLPVNSGSGGPNDLSFGRGDVMPSAQADKTGTMLSDGAIYSSIDFTTKGGFGSPSPASQATPYATTQILHSNSIHELAVDLPEAQWKTSLQAKQEMASLGYSLPDKNSCNNTLLFIPDYRLADGLSNRMPHNQSQDFSTTSSHNSSDRSNSLSGGKGGKKKKTKANVKPTKMNGSNWANVPLPPPPIHPLPGTEMDHYASEHHDGGGYDSDGWGPPLPVQTYLHQGLEDELEEEEERVPTPPIRGVASSPAAVSFGKQSTATLSPSPREEMQPMLQAHLDELTRAYQLDMAKQTWHMQGGPYPPQAPAPPVGYVSSTLVSDIETDLPDEDDDDEEDEGYEMSPPIRGTEHTPGSSMDNLDSSVTDKGLPHRPRPASPFSTDGSTVGTHSLGHQRAARPTRKHKGPGTVPNRRDASADDLPPPPEPPPCQTARIPGSRNVPHPCDRKASSLERQPMMGLEEMRSSLDRQARTSLDRHQQVQDRLGSIERGDESRRGQKNGPFSEEALLPYNKPSFPSPGGHSSSGTASSKGSTGPRKGEGTRGQHQRSTTEQGESSYMGTNAQFSGELCESEQVQE

Target ORF information:

RefSeq Version	XM_017351008.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio roundabout, axon guidance receptor, homolog 2 (Drosophila) (robo2), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017351008.1

ORF Insert Sequence:

ATGGGTCCTT TAACACACCT TTTACTCTGT GGATTAGTTT ACTTTAACCA AGTGGATGGA 
TCCCGTCTGC GGCAGGAAGA TTCTCCACCT CGGATTGTAG AACACCCCTC CGATCTGATC 
GTGTCAAAGG GCGAGCCAGC CACCCTAAAC TGCAAGGCGG AGGGTCGGCC AACTCCCACC 
GTGGAGTGGT ACAAGGATGG AGAACGAGTT GAAACGGACA AAGATGACCC TCGATCTCAC 
CGCATGCTCC TTCCCAGTGG TTCGCTCTTC TTCCTGCGTA TCGTACATGG ACGCCGCAGC 
AAACCTGATG AGGGGGCGTA CGTCTGTGTG GCACGCAACT ACTTGGGGGA AGCAGTCAGT 
CGAAACGCCT CGCTGGAAGT AGCATTGTTA CGGGATGATT TCCGGCAGAA CCCTACAGAT 
GTGGTGGTCG CTGCTGGAGA GCCGGCCATC CTGGAGTGTG TCCCTCCCAG AGGACACCCG 
GAGCCCACCA TCTACTGGAA GAAAGACAAA GTCCGAATTG ATGAGAAGGA TGACAGGATC 
AAGATCCGTG GAGGGAAGCT GATGATCTCT AACACCAGGA AAAGCGACGC TGGAATGTAC 
ATCTGCGTGG GCACCAACAT GGTCGGCGAA AGAGACAGTG AAACAGCCCA GGTCACTGTG 
TTCGAGAGGC CCACGTTCCT GCGGCGTCCC ATTAACCAGG TGGTTCTGGA GGAGGAGGCT 
GTGGAATTTC GATGTCAGGT GCAGGGAGAC CCGCAGCCGT CCATCCGCTG GAAAAAAGAC 
GACGTTGAAA TCCCCCGCGG CAGGTACGAT ATGAAATTTG ATAAAGATGA CTTCACGCTG 
AGAATAAAGA AGGCAACAGC GAGTGATGAA GGAACCTTCA CCTGTGTAGC GGAGAACCGA 
GTGGGCAAAA TGGAGGCCTC CGCAACCCTC ACGGTCAGAG CTCGCCCTGT CGCTGCACCG 
CAGTTCGTGA TCAGGCCGCG GGATCAGATT GTGGCACAGG GACGCACCGC CAGCTTCCCC 
TGCGAGACTA AAGGGAACCC GCAGCCGGCA GTCTTCTGGC AGAAAGAAGG AAGTCAGAAT 
CTCCTGTTTC CAAATCAACC CCAGCAGCCC AACAGTCGTT TCTCGGTGTC GCCCAGTGGA 
GATCTAACCA TCACTACGGT CCAGCGGGCA GATGCGGGCT ATTATATCTG CCAGGCCCTG 
ACGGTGGCGG GCAGCATCCT GGCCAAAGCA CAGCTTGAGG TCACAGACGT GTTGACCGAT 
CGCCCTCCAC CCATCATCCG GCAGGGTCCA GCTAACCAGA CTCTTGGTGT AGACAGTGTG 
GCTCTTTTGA GGTGCCATGC ATCCGGAGAA CCAGTCCCAA CCATCAGCTG GCTGAAGGAC 
GGGGTCAGTC TGCTGGGCAA AGACCCTCGG ATGTCCCTGC AAGACCTCGG CAGCCTGCAG 
ATCAAGGGTT TACGGCTCTC TGATTCTGGC ATTTATACTT GTGTGGCTGC AAGTTCTAGT 
GGGGAAACAT CATGGAGTGC TTTCTTGGAG GTCAAAGAGG CTGGGAGTCT TGTTGTAGTT 
AAAGATCGGG ATGAGAACGA GCTTCCAGGA CCTCCTTCCA AACCTCAGGT CACAGATGTC 
ACTAAAAACA GCGTGTCATT ATCCTGGCAG CCAGGCCTTC CCGGAGCATC AGCCATTTCT 
ACATATGTCA TAGAAGCATT CAGCCAATCG GTGAGCAACA GCTGGCAAAC TGTTGCCGAT 
CACGCCAAGA CAACGCAGTA CACCATTAAG GGTCTCCGCC CTAACACCAT CTACCTGTTC 
ATGGTGCGAG CGGTCAACGT CCAAGGCCTG AGCGACCCAA GCCCCATGTC TGAACCCGTC 
CGCACTCAAG ATATAAGTCC TCCAGCGCAG GGTGTGGATC ACAGACATGT GCAAAAGGAG 
CTGGGAGAAG TTATTGTGCG GCTACACAAC CCTGTGGTCC TTAGCCCTAC CACCATACAG 
GTCACATGGA CGGTTGACCG CCAGTCTCAG TTCATCCAGG GCTACAGGGT TTTGTACAGG 
CAGATGTCAG GCCTGTCTTC TCCAGGAGCC TGGCAAACTC AGGATGTGAA AGTCCCCTCA 
GAGAGGAGCA TGGTGCTCTC GGCTCTAAAG AAAGGCATCG TCTACGAGAT CAAAGTGCGT 
CCATATTTCA ATGAGTTTCA GGGCATGGAC AGCGAATCCC GAACCGCAAG AACAACAGAG 
GAAGCGCCCA GTGCTCCTCC TCAGCAGGTG ACTGTACTCA CTGTGGGCAA CCAGAACAGC 
ACATCTATTA GCATCTCCTG GGACCCTCCG CCTGCAGAAC ACCAGAACGG CATCATACAG 
GAGTACAAGA TCTGGTGTTT GGGGAATGAG ACCCGTTTTC ATGTGAATAA GACGGTGGAC 
GCAGCGATCC GCTCAGTGGT GGTTGGGGGT CTCCAGGCGG GGGTCCAGTA TCGGGTGGAG 
GTGGCCGCTA GCACCAGCGC CGGGGTCGGA GTGAAGAGCG AACCACAAAC CATCATCATT 
GGTAAAGACT TCCGCGATGT GATGATTAGC AGGAATAACA GCATCACAGA GCAGATCACG 
GATGTGGTGA AACAGCCGGC CTTCATCGCG GGCATTGGCG GGGCCTGCTG GGTCATTCTG 
ATGGGCTTCA GCATCTGGCT CTACTGGAGG AGGAAGAAGA GAAAGGGACT CAGCAATTAC 
GCAGTTCAGT CGTTCACCTT CACCCCTGCA GTGACGTTCC AGCGTGTTGA TGGAGGCTTG 
ATGAGCAACG GGAGTCGTCC TGGTCTGCTG AACGCCAGTG ATTCGGGTTA CCCGTGGCTC 
GCCGACTCCT GGCCTCCCAG CCTGCCTGTC AACAGCGGAT CTGGAGGACC CAATGACCTC 
AGCTTTGGCC GTGGAGATGT GATGCCCAGT GCTCAGGCTG ATAAGACGGG TACCATGCTT 
TCGGATGGAG CCATTTACAG CAGCATCGAC TTCACCACCA AAGGCGGATT CGGTAGCCCA 
AGCCCAGCCT CCCAGGCCAC GCCCTATGCC ACCACACAGA TCCTCCACTC CAACAGCATC 
CATGAGCTGG CAGTGGATCT TCCTGAAGCC CAGTGGAAGA CCTCCCTACA GGCCAAGCAG 
GAGATGGCCA GCCTGGGTTA CTCACTGCCC GACAAAAACT CCTGCAATAA CACACTCCTT 
TTCATTCCTG ACTACCGATT GGCTGACGGA CTGTCTAATA GAATGCCACA CAACCAATCG 
CAGGATTTCA GCACCACCAG CTCCCACAAC AGCTCAGATC GGAGCAACAG TCTGTCAGGT 
GGAAAAGGTG GTAAGAAGAA GAAGACCAAA GCAAACGTCA AGCCCACCAA GATGAATGGC 
TCCAACTGGG CGAACGTTCC ACTTCCTCCT CCTCCCATCC ACCCTCTTCC TGGCACAGAG 
ATGGACCACT ACGCCTCAGA GCACCATGAT GGAGGAGGAT ATGATAGTGA TGGTTGGGGC 
CCACCATTGC CAGTGCAGAC GTACCTCCAT CAGGGTTTAG AGGATGAGCT GGAGGAAGAG 
GAAGAGCGGG TGCCAACTCC TCCCATTAGG GGTGTGGCTT CTTCTCCTGC TGCAGTTTCC 
TTTGGCAAAC AGTCCACAGC CACACTGAGC CCATCTCCAC GTGAAGAAAT GCAGCCCATG 
CTCCAAGCCC ACCTAGATGA GCTTACCCGT GCTTATCAAT TGGACATGGC TAAACAGACT 
TGGCACATGC AGGGTGGGCC TTACCCGCCC CAGGCCCCCG CACCACCCGT GGGCTACGTC 
TCCAGCACCT TAGTGTCTGA CATCGAGACC GATCTCCCTG ATGAAGATGA CGATGATGAG 
GAGGATGAAG GATACGAGAT GTCGCCACCA ATTAGGGGCA CTGAACACAC GCCAGGCTCC 
AGCATGGACA ACTTAGACAG CTCCGTTACG GATAAAGGTC TACCTCATAG ACCTCGTCCC 
GCTAGCCCGT TCTCCACAGA TGGAAGCACG GTGGGCACAC ACAGTCTGGG GCACCAGCGG 
GCAGCAAGGC CCACACGTAA ACACAAAGGC CCTGGCACTG TCCCGAACCG CCGTGATGCC 
AGTGCGGATG ATCTGCCTCC ACCTCCAGAG CCTCCACCCT GCCAGACAGC TCGCATCCCC 
GGGTCGAGGA ACGTTCCCCA TCCCTGCGAC CGCAAAGCTT CCTCTCTGGA GAGGCAGCCT 
ATGATGGGCC TTGAGGAAAT GAGAAGTTCC TTGGACCGGC AGGCCCGAAC CTCATTGGAC 
CGCCACCAAC AAGTCCAGGA CAGACTGGGG TCCATAGAGA GAGGAGATGA AAGCAGAAGA 
GGACAGAAAA ATGGTCCCTT TTCAGAAGAG GCCCTATTGC CATACAACAA ACCATCATTC 
CCATCCCCTG GTGGGCACAG CTCATCTGGG ACGGCCTCTT CTAAAGGGTC AACGGGTCCT 
CGCAAAGGAG AAGGGACGAG GGGACAGCAC CAGCGCTCCA CCACGGAACA AGGAGAAAGC 
AGCTACATGG GGACCAACGC GCAGTTTTCC GGAGAGTTAT GTGAGTCTGA ACAAGTTCAA 
GAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

robo2 ( XM_017351009.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017206498.1 Danio rerio roundabout, axon guidance receptor, homolog 2 (Drosophila) (robo2), transcript variant X11, mRNA.

Price & Availability↑

CloneID	ODa55127
Clone ID Related Accession (Same CDS sequence)	XM_017351009.1
Accession Version	XM_017351009.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4761bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	roundabout homolog 2 isoform X11
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCCTCC TCTCTCCTGT GAGACTCACT TTATTTCTGT TGGTCTTCCT GGATGTTCCT 
CATCCGTCCC TTCAGGGCAG GAATAAGGGC AGATGGAACA AGGGGGGATC CCGTCTGCGG 
CAGGAAGATT CTCCACCTCG GATTGTAGAA CACCCCTCCG ATCTGATCGT GTCAAAGGGC 
GAGCCAGCCA CCCTAAACTG CAAGGCGGAG GGTCGGCCAA CTCCCACCGT GGAGTGGTAC 
AAGGATGGAG AACGAGTTGA AACGGACAAA GATGACCCTC GATCTCACCG CATGCTCCTT 
CCCAGTGGTT CGCTCTTCTT CCTGCGTATC GTACATGGAC GCCGCAGCAA ACCTGATGAG 
GGGGCGTACG TCTGTGTGGC ACGCAACTAC TTGGGGGAAG CAGTCAGTCG AAACGCCTCG 
CTGGAAGTAG CATTGTTACG GGATGATTTC CGGCAGAACC CTACAGATGT GGTGGTCGCT 
GCTGGAGAGC CGGCCATCCT GGAGTGTGTC CCTCCCAGAG GACACCCGGA GCCCACCATC 
TACTGGAAGA AAGACAAAGT CCGAATTGAT GAGAAGGATG ACAGGATCAA GATCCGTGGA 
GGGAAGCTGA TGATCTCTAA CACCAGGAAA AGCGACGCTG GAATGTACAT CTGCGTGGGC 
ACCAACATGG TCGGCGAAAG AGACAGTGAA ACAGCCCAGG TCACTGTGTT CGAGAGGCCC 
ACGTTCCTGC GGCGTCCCAT TAACCAGGTG GTTCTGGAGG AGGAGGCTGT GGAATTTCGA 
TGTCAGGTGC AGGGAGACCC GCAGCCGTCC ATCCGCTGGA AAAAAGACGA CGTTGAAATC 
CCCCGCGGCA GGTACGATAT GAAATTTGAT AAAGATGACT TCACGCTGAG AATAAAGAAG 
GCAACAGCGA GTGATGAAGG AACCTTCACC TGTGTAGCGG AGAACCGAGT GGGCAAAATG 
GAGGCCTCCG CAACCCTCAC GGTCAGAGCT GCACCGCAGT TCGTGATCAG GCCGCGGGAT 
CAGATTGTGG CACAGGGACG CACCGCCAGC TTCCCCTGCG AGACTAAAGG GAACCCGCAG 
CCGGCAGTCT TCTGGCAGAA AGAAGGAAGT CAGAATCTCC TGTTTCCAAA TCAACCCCAG 
CAGCCCAACA GTCGTTTCTC GGTGTCGCCC AGTGGAGATC TAACCATCAC TACGGTCCAG 
CGGGCAGATG CGGGCTATTA TATCTGCCAG GCCCTGACGG TGGCGGGCAG CATCCTGGCC 
AAAGCACAGC TTGAGGTCAC AGACGTGTTG ACCGATCGCC CTCCACCCAT CATCCGGCAG 
GGTCCAGCTA ACCAGACTCT TGGTGTAGAC AGTGTGGCTC TTTTGAGGTG CCATGCATCC 
GGAGAACCAG TCCCAACCAT CAGCTGGCTG AAGGACGGGG TCAGTCTGCT GGGCAAAGAC 
CCTCGGATGT CCCTGCAAGA CCTCGGCAGC CTGCAGATCA AGGGTTTACG GCTCTCTGAT 
TCTGGCATTT ATACTTGTGT GGCTGCAAGT TCTAGTGGGG AAACATCATG GAGTGCTTTC 
TTGGAGGTCA AAGAGGCTGG GAGTCTTGTT GTAGTTAAAG ATCGGGATGA GAACGAGCTT 
CCAGGACCTC CTTCCAAACC TCAGGTCACA GATGTCACTA AAAACAGCGT GTCATTATCC 
TGGCAGCCAG GCCTTCCCGG AGCATCAGCC ATTTCTACAT ATGTCATAGA AGCATTCAGC 
CAATCGGTGA GCAACAGCTG GCAAACTGTT GCCGATCACG CCAAGACAAC GCAGTACACC 
ATTAAGGGTC TCCGCCCTAA CACCATCTAC CTGTTCATGG TGCGAGCGGT CAACGTCCAA 
GGCCTGAGCG ACCCAAGCCC CATGTCTGAA CCCGTCCGCA CTCAAGATAT AAGTCCTCCA 
GCGCAGGGTG TGGATCACAG ACATGTGCAA AAGGAGCTGG GAGAAGTTAT TGTGCGGCTA 
CACAACCCTG TGGTCCTTAG CCCTACCACC ATACAGGTCA CATGGACGGT TGACCGCCAG 
TCTCAGTTCA TCCAGGGCTA CAGGGTTTTG TACAGGCAGA TGTCAGGCCT GTCTTCTCCA 
GGAGCCTGGC AAACTCAGGA TGTGAAAGTC CCCTCAGAGA GGAGCATGGT GCTCTCGGCT 
CTAAAGAAAG GCATCGTCTA CGAGATCAAA GTGCGTCCAT ATTTCAATGA GTTTCAGGGC 
ATGGACAGCG AATCCCGAAC CGCAAGAACA ACAGAGGAAG CGCCCAGTGC TCCTCCTCAG 
CAGGTGACTG TACTCACTGT GGGCAACCAG AACAGCACAT CTATTAGCAT CTCCTGGGAC 
CCTCCGCCTG CAGAACACCA GAACGGCATC ATACAGGAGT ACAAGATCTG GTGTTTGGGG 
AATGAGACCC GTTTTCATGT GAATAAGACG GTGGACGCAG CGATCCGCTC AGTGGTGGTT 
GGGGGTCTCC AGGCGGGGGT CCAGTATCGG GTGGAGGTGG CCGCTAGCAC CAGCGCCGGG 
GTCGGAGTGA AGAGCGAACC ACAAACCATC ATCATTGGTA AAGACTTCCG CGATGTGATG 
ATTAGCAGGA ATAACAGCAT CACAGAGCAG ATCACGGATG TGGTGAAACA GCCGGCCTTC 
ATCGCGGGCA TTGGCGGGGC CTGCTGGGTC ATTCTGATGG GCTTCAGCAT CTGGCTCTAC 
TGGAGGAGGA AGAAGAGAAA GGGACTCAGC AATTACGCAG TGACGTTCCA GCGTGTTGAT 
GGAGGCTTGA TGAGCAACGG GAGTCGTCCT GGTCTGCTGA ACGCCAGTGA TTCGGGTTAC 
CCGTGGCTCG CCGACTCCTG GCCTCCCAGC CTGCCTGTCA ACAGCGGATC TGGAGGACCC 
AATGACCTCA GCTTTGGCCG TGGAGATGTG ATGCCCAGTG CTCAGGCTGA TAAGACGGGT 
ACCATGCTTT CGGATGGAGC CATTTACAGC AGCATCGACT TCACCACCAA AGGCGGATTC 
GGTAGCCCAA GCCCAGCCTC CCAGGCCACG CCCTATGCCA CCACACAGAT CCTCCACTCC 
AACAGCATCC ATGAGCTGGC AGTGGATCTT CCTGAAGCCC AGTGGAAGAC CTCCCTACAG 
GCCAAGCAGG AGATGGCCAG CCTGGGTTAC TCACTGCCCG ACAAAAACTC CTGCAATAAC 
ACACTCCTTT TCATTCCTGA CTACCGATTG GCTGACGGAC TGTCTAATAG AATGCCACAC 
AACCAATCGC AGGATTTCAG CACCACCAGC TCCCACAACA GCTCAGATCG GAGCAACAGT 
CTGTCAGGTG GAAAAGGTGG TAAGAAGAAG AAGACCAAAG CAAACGTCAA GCCCACCAAG 
ATGAATGGCT CCAACTGGGC GAACGTTCCA CTTCCTCCTC CTCCCATCCA CCCTCTTCCT 
GGCACAGAGA TGGACCACTA CGCCTCAGAG CACCATGATG GAGGAGGATA TGATAGTGAT 
GGTTGGGGCC CACCATTGCC AGTGCAGACG TACCTCCATC AGGGTTTAGA GGATGAGCTG 
GAGGAAGAGG AAGAGCGGGT GCCAACTCCT CCCATTAGGG GTGTGGCTTC TTCTCCTGCT 
GCAGTTTCCT TTGGCAAACA GTCCACAGCC ACACTGAGCC CATCTCCACG TGAAGAAATG 
CAGCCCATGC TCCAAGCCCA CCTAGATGAG CTTACCCGTG CTTATCAATT GGACATGGCT 
AAACAGACTT GGCACATGCA GGGTGGGCCT TACCCGCCCC AGGCCCCCGC ACCACCCGTG 
GGCTACGTCT CCAGCACCTT AGTGTCTGAC ATCGAGACCG ATCTCCCTGA TGAAGATGAC 
GATGATGAGG AGGATGAAGG ATACGAGATG TCGCCACCAA TTAGGGGCAC TGAACACACG 
CCAGGCTCCA GCATGGACAA CTTAGACAGC TCCGTTACGG GCTCTATGGT GAACGGCTGG 
GGCTCGGCTT CAGAAGATGA TGACAGAAAC TTCTCCAGCA GGAGGTCTAG TGCCGGCAGC 
TCTTCTGACG GCTCGCTCTT CACCCATTGC AGCTTTGCGC GAGCCCTGGT GGCAGCCGCA 
GACAAGGCTG GTTACCGTCT GGATGGCACC AGCCTGAGCA AAAGAGATAA AGGTCTACCT 
CATAGACCTC GTCCCGCTAG CCCGTTCTCC ACAGATGGAA GCACGGTGGG CACACACAGT 
CTGGGGCACC AGCGGGCAGC AAGGCCCACA CGTAAACACA AAGGCCCTGG CACTGTCCCG 
AACCGCCGTG ATGCCAGTGC GGATGATCTG CCTCCACCTC CAGAGCCTCC ACCCTGCCAG 
ACAGCTCGCA TCCCCGGGTC GAGGAACGTT CCCCATCCCT GCGACCGCAA AGCTTCCTCT 
CTGGAGAGGC AGCCTATGAT GGGCCTTGAG GAAATGAGAA GTTCCTTGGA CCGGCAGGCC 
CGAACCTCAT TGGACCGCCA CCAACAAGTC CAGGACAGAC TGGGGTCCAT AGAGAGAGGA 
GATGAAAGCA GAAGAGGACA GAAAAATGGT CCCTTTTCAG AAGAGGCCCT ATTGCCATAC 
AACAAACCAT CATTCCCATC CCCTGGTGGG CACAGCTCAT CTGGGACGGC CTCTTCTAAA 
GGGTCAACGG GTCCTCGCAA AGGAGAAGGG ACGAGGGGAC AGCACCAGCG CTCCACCACG 
GAACAAGGAG AAAGCAGCTA CATGGGGACC AACGCGCAGT TTTCCGGAGA GTTATGTGAG 
TCTGAACAAG TTCAAGAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017206498.1
CDS	680..5440
Translation	MFLLSPVRLTLFLLVFLDVPHPSLQGRNKGRWNKGGSRLRQEDSPPRIVEHPSDLIVSKGEPATLNCKAEGRPTPTVEWYKDGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRRSKPDEGAYVCVARNYLGEAVSRNASLEVALLRDDFRQNPTDVVVAAGEPAILECVPPRGHPEPTIYWKKDKVRIDEKDDRIKIRGGKLMISNTRKSDAGMYICVGTNMVGERDSETAQVTVFERPTFLRRPINQVVLEEEAVEFRCQVQGDPQPSIRWKKDDVEIPRGRYDMKFDKDDFTLRIKKATASDEGTFTCVAENRVGKMEASATLTVRAAPQFVIRPRDQIVAQGRTASFPCETKGNPQPAVFWQKEGSQNLLFPNQPQQPNSRFSVSPSGDLTITTVQRADAGYYICQALTVAGSILAKAQLEVTDVLTDRPPPIIRQGPANQTLGVDSVALLRCHASGEPVPTISWLKDGVSLLGKDPRMSLQDLGSLQIKGLRLSDSGIYTCVAASSSGETSWSAFLEVKEAGSLVVVKDRDENELPGPPSKPQVTDVTKNSVSLSWQPGLPGASAISTYVIEAFSQSVSNSWQTVADHAKTTQYTIKGLRPNTIYLFMVRAVNVQGLSDPSPMSEPVRTQDISPPAQGVDHRHVQKELGEVIVRLHNPVVLSPTTIQVTWTVDRQSQFIQGYRVLYRQMSGLSSPGAWQTQDVKVPSERSMVLSALKKGIVYEIKVRPYFNEFQGMDSESRTARTTEEAPSAPPQQVTVLTVGNQNSTSISISWDPPPAEHQNGIIQEYKIWCLGNETRFHVNKTVDAAIRSVVVGGLQAGVQYRVEVAASTSAGVGVKSEPQTIIIGKDFRDVMISRNNSITEQITDVVKQPAFIAGIGGACWVILMGFSIWLYWRRKKRKGLSNYAVTFQRVDGGLMSNGSRPGLLNASDSGYPWLADSWPPSLPVNSGSGGPNDLSFGRGDVMPSAQADKTGTMLSDGAIYSSIDFTTKGGFGSPSPASQATPYATTQILHSNSIHELAVDLPEAQWKTSLQAKQEMASLGYSLPDKNSCNNTLLFIPDYRLADGLSNRMPHNQSQDFSTTSSHNSSDRSNSLSGGKGGKKKKTKANVKPTKMNGSNWANVPLPPPPIHPLPGTEMDHYASEHHDGGGYDSDGWGPPLPVQTYLHQGLEDELEEEEERVPTPPIRGVASSPAAVSFGKQSTATLSPSPREEMQPMLQAHLDELTRAYQLDMAKQTWHMQGGPYPPQAPAPPVGYVSSTLVSDIETDLPDEDDDDEEDEGYEMSPPIRGTEHTPGSSMDNLDSSVTGSMVNGWGSASEDDDRNFSSRRSSAGSSSDGSLFTHCSFARALVAAADKAGYRLDGTSLSKRDKGLPHRPRPASPFSTDGSTVGTHSLGHQRAARPTRKHKGPGTVPNRRDASADDLPPPPEPPPCQTARIPGSRNVPHPCDRKASSLERQPMMGLEEMRSSLDRQARTSLDRHQQVQDRLGSIERGDESRRGQKNGPFSEEALLPYNKPSFPSPGGHSSSGTASSKGSTGPRKGEGTRGQHQRSTTEQGESSYMGTNAQFSGELCESEQVQE

Target ORF information:

RefSeq Version	XM_017351009.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio roundabout, axon guidance receptor, homolog 2 (Drosophila) (robo2), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017351009.1

ORF Insert Sequence:

ATGTTCCTCC TCTCTCCTGT GAGACTCACT TTATTTCTGT TGGTCTTCCT GGATGTTCCT 
CATCCGTCCC TTCAGGGCAG GAATAAGGGC AGATGGAACA AGGGGGGATC CCGTCTGCGG 
CAGGAAGATT CTCCACCTCG GATTGTAGAA CACCCCTCCG ATCTGATCGT GTCAAAGGGC 
GAGCCAGCCA CCCTAAACTG CAAGGCGGAG GGTCGGCCAA CTCCCACCGT GGAGTGGTAC 
AAGGATGGAG AACGAGTTGA AACGGACAAA GATGACCCTC GATCTCACCG CATGCTCCTT 
CCCAGTGGTT CGCTCTTCTT CCTGCGTATC GTACATGGAC GCCGCAGCAA ACCTGATGAG 
GGGGCGTACG TCTGTGTGGC ACGCAACTAC TTGGGGGAAG CAGTCAGTCG AAACGCCTCG 
CTGGAAGTAG CATTGTTACG GGATGATTTC CGGCAGAACC CTACAGATGT GGTGGTCGCT 
GCTGGAGAGC CGGCCATCCT GGAGTGTGTC CCTCCCAGAG GACACCCGGA GCCCACCATC 
TACTGGAAGA AAGACAAAGT CCGAATTGAT GAGAAGGATG ACAGGATCAA GATCCGTGGA 
GGGAAGCTGA TGATCTCTAA CACCAGGAAA AGCGACGCTG GAATGTACAT CTGCGTGGGC 
ACCAACATGG TCGGCGAAAG AGACAGTGAA ACAGCCCAGG TCACTGTGTT CGAGAGGCCC 
ACGTTCCTGC GGCGTCCCAT TAACCAGGTG GTTCTGGAGG AGGAGGCTGT GGAATTTCGA 
TGTCAGGTGC AGGGAGACCC GCAGCCGTCC ATCCGCTGGA AAAAAGACGA CGTTGAAATC 
CCCCGCGGCA GGTACGATAT GAAATTTGAT AAAGATGACT TCACGCTGAG AATAAAGAAG 
GCAACAGCGA GTGATGAAGG AACCTTCACC TGTGTAGCGG AGAACCGAGT GGGCAAAATG 
GAGGCCTCCG CAACCCTCAC GGTCAGAGCT GCACCGCAGT TCGTGATCAG GCCGCGGGAT 
CAGATTGTGG CACAGGGACG CACCGCCAGC TTCCCCTGCG AGACTAAAGG GAACCCGCAG 
CCGGCAGTCT TCTGGCAGAA AGAAGGAAGT CAGAATCTCC TGTTTCCAAA TCAACCCCAG 
CAGCCCAACA GTCGTTTCTC GGTGTCGCCC AGTGGAGATC TAACCATCAC TACGGTCCAG 
CGGGCAGATG CGGGCTATTA TATCTGCCAG GCCCTGACGG TGGCGGGCAG CATCCTGGCC 
AAAGCACAGC TTGAGGTCAC AGACGTGTTG ACCGATCGCC CTCCACCCAT CATCCGGCAG 
GGTCCAGCTA ACCAGACTCT TGGTGTAGAC AGTGTGGCTC TTTTGAGGTG CCATGCATCC 
GGAGAACCAG TCCCAACCAT CAGCTGGCTG AAGGACGGGG TCAGTCTGCT GGGCAAAGAC 
CCTCGGATGT CCCTGCAAGA CCTCGGCAGC CTGCAGATCA AGGGTTTACG GCTCTCTGAT 
TCTGGCATTT ATACTTGTGT GGCTGCAAGT TCTAGTGGGG AAACATCATG GAGTGCTTTC 
TTGGAGGTCA AAGAGGCTGG GAGTCTTGTT GTAGTTAAAG ATCGGGATGA GAACGAGCTT 
CCAGGACCTC CTTCCAAACC TCAGGTCACA GATGTCACTA AAAACAGCGT GTCATTATCC 
TGGCAGCCAG GCCTTCCCGG AGCATCAGCC ATTTCTACAT ATGTCATAGA AGCATTCAGC 
CAATCGGTGA GCAACAGCTG GCAAACTGTT GCCGATCACG CCAAGACAAC GCAGTACACC 
ATTAAGGGTC TCCGCCCTAA CACCATCTAC CTGTTCATGG TGCGAGCGGT CAACGTCCAA 
GGCCTGAGCG ACCCAAGCCC CATGTCTGAA CCCGTCCGCA CTCAAGATAT AAGTCCTCCA 
GCGCAGGGTG TGGATCACAG ACATGTGCAA AAGGAGCTGG GAGAAGTTAT TGTGCGGCTA 
CACAACCCTG TGGTCCTTAG CCCTACCACC ATACAGGTCA CATGGACGGT TGACCGCCAG 
TCTCAGTTCA TCCAGGGCTA CAGGGTTTTG TACAGGCAGA TGTCAGGCCT GTCTTCTCCA 
GGAGCCTGGC AAACTCAGGA TGTGAAAGTC CCCTCAGAGA GGAGCATGGT GCTCTCGGCT 
CTAAAGAAAG GCATCGTCTA CGAGATCAAA GTGCGTCCAT ATTTCAATGA GTTTCAGGGC 
ATGGACAGCG AATCCCGAAC CGCAAGAACA ACAGAGGAAG CGCCCAGTGC TCCTCCTCAG 
CAGGTGACTG TACTCACTGT GGGCAACCAG AACAGCACAT CTATTAGCAT CTCCTGGGAC 
CCTCCGCCTG CAGAACACCA GAACGGCATC ATACAGGAGT ACAAGATCTG GTGTTTGGGG 
AATGAGACCC GTTTTCATGT GAATAAGACG GTGGACGCAG CGATCCGCTC AGTGGTGGTT 
GGGGGTCTCC AGGCGGGGGT CCAGTATCGG GTGGAGGTGG CCGCTAGCAC CAGCGCCGGG 
GTCGGAGTGA AGAGCGAACC ACAAACCATC ATCATTGGTA AAGACTTCCG CGATGTGATG 
ATTAGCAGGA ATAACAGCAT CACAGAGCAG ATCACGGATG TGGTGAAACA GCCGGCCTTC 
ATCGCGGGCA TTGGCGGGGC CTGCTGGGTC ATTCTGATGG GCTTCAGCAT CTGGCTCTAC 
TGGAGGAGGA AGAAGAGAAA GGGACTCAGC AATTACGCAG TGACGTTCCA GCGTGTTGAT 
GGAGGCTTGA TGAGCAACGG GAGTCGTCCT GGTCTGCTGA ACGCCAGTGA TTCGGGTTAC 
CCGTGGCTCG CCGACTCCTG GCCTCCCAGC CTGCCTGTCA ACAGCGGATC TGGAGGACCC 
AATGACCTCA GCTTTGGCCG TGGAGATGTG ATGCCCAGTG CTCAGGCTGA TAAGACGGGT 
ACCATGCTTT CGGATGGAGC CATTTACAGC AGCATCGACT TCACCACCAA AGGCGGATTC 
GGTAGCCCAA GCCCAGCCTC CCAGGCCACG CCCTATGCCA CCACACAGAT CCTCCACTCC 
AACAGCATCC ATGAGCTGGC AGTGGATCTT CCTGAAGCCC AGTGGAAGAC CTCCCTACAG 
GCCAAGCAGG AGATGGCCAG CCTGGGTTAC TCACTGCCCG ACAAAAACTC CTGCAATAAC 
ACACTCCTTT TCATTCCTGA CTACCGATTG GCTGACGGAC TGTCTAATAG AATGCCACAC 
AACCAATCGC AGGATTTCAG CACCACCAGC TCCCACAACA GCTCAGATCG GAGCAACAGT 
CTGTCAGGTG GAAAAGGTGG TAAGAAGAAG AAGACCAAAG CAAACGTCAA GCCCACCAAG 
ATGAATGGCT CCAACTGGGC GAACGTTCCA CTTCCTCCTC CTCCCATCCA CCCTCTTCCT 
GGCACAGAGA TGGACCACTA CGCCTCAGAG CACCATGATG GAGGAGGATA TGATAGTGAT 
GGTTGGGGCC CACCATTGCC AGTGCAGACG TACCTCCATC AGGGTTTAGA GGATGAGCTG 
GAGGAAGAGG AAGAGCGGGT GCCAACTCCT CCCATTAGGG GTGTGGCTTC TTCTCCTGCT 
GCAGTTTCCT TTGGCAAACA GTCCACAGCC ACACTGAGCC CATCTCCACG TGAAGAAATG 
CAGCCCATGC TCCAAGCCCA CCTAGATGAG CTTACCCGTG CTTATCAATT GGACATGGCT 
AAACAGACTT GGCACATGCA GGGTGGGCCT TACCCGCCCC AGGCCCCCGC ACCACCCGTG 
GGCTACGTCT CCAGCACCTT AGTGTCTGAC ATCGAGACCG ATCTCCCTGA TGAAGATGAC 
GATGATGAGG AGGATGAAGG ATACGAGATG TCGCCACCAA TTAGGGGCAC TGAACACACG 
CCAGGCTCCA GCATGGACAA CTTAGACAGC TCCGTTACGG GCTCTATGGT GAACGGCTGG 
GGCTCGGCTT CAGAAGATGA TGACAGAAAC TTCTCCAGCA GGAGGTCTAG TGCCGGCAGC 
TCTTCTGACG GCTCGCTCTT CACCCATTGC AGCTTTGCGC GAGCCCTGGT GGCAGCCGCA 
GACAAGGCTG GTTACCGTCT GGATGGCACC AGCCTGAGCA AAAGAGATAA AGGTCTACCT 
CATAGACCTC GTCCCGCTAG CCCGTTCTCC ACAGATGGAA GCACGGTGGG CACACACAGT 
CTGGGGCACC AGCGGGCAGC AAGGCCCACA CGTAAACACA AAGGCCCTGG CACTGTCCCG 
AACCGCCGTG ATGCCAGTGC GGATGATCTG CCTCCACCTC CAGAGCCTCC ACCCTGCCAG 
ACAGCTCGCA TCCCCGGGTC GAGGAACGTT CCCCATCCCT GCGACCGCAA AGCTTCCTCT 
CTGGAGAGGC AGCCTATGAT GGGCCTTGAG GAAATGAGAA GTTCCTTGGA CCGGCAGGCC 
CGAACCTCAT TGGACCGCCA CCAACAAGTC CAGGACAGAC TGGGGTCCAT AGAGAGAGGA 
GATGAAAGCA GAAGAGGACA GAAAAATGGT CCCTTTTCAG AAGAGGCCCT ATTGCCATAC 
AACAAACCAT CATTCCCATC CCCTGGTGGG CACAGCTCAT CTGGGACGGC CTCTTCTAAA 
GGGTCAACGG GTCCTCGCAA AGGAGAAGGG ACGAGGGGAC AGCACCAGCG CTCCACCACG 
GAACAAGGAG AAAGCAGCTA CATGGGGACC AACGCGCAGT TTTCCGGAGA GTTATGTGAG 
TCTGAACAAG TTCAAGAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

robo2 ( XM_017351010.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017206499.1 Danio rerio roundabout, axon guidance receptor, homolog 2 (Drosophila) (robo2), transcript variant X12, mRNA.

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CloneID	ODa55128
Clone ID Related Accession (Same CDS sequence)	XM_017351010.1
Accession Version	XM_017351010.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4635bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	roundabout homolog 2 isoform X12
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCCTCC TCTCTCCTGT GAGACTCACT TTATTTCTGT TGGTCTTCCT GGATGTTCCT 
CATCCGTCCC TTCAGGGCAG GAATAAGGGC AGATGGAACA AGGGGGGATC CCGTCTGCGG 
CAGGAAGATT CTCCACCTCG GATTGTAGAA CACCCCTCCG ATCTGATCGT GTCAAAGGGC 
GAGCCAGCCA CCCTAAACTG CAAGGCGGAG GGTCGGCCAA CTCCCACCGT GGAGTGGTAC 
AAGGATGGAG AACGAGTTGA AACGGACAAA GATGACCCTC GATCTCACCG CATGCTCCTT 
CCCAGTGGTT CGCTCTTCTT CCTGCGTATC GTACATGGAC GCCGCAGCAA ACCTGATGAG 
GGGGCGTACG TCTGTGTGGC ACGCAACTAC TTGGGGGAAG CAGTCAGTCG AAACGCCTCG 
CTGGAAGTAG CATTGTTACG GGATGATTTC CGGCAGAACC CTACAGATGT GGTGGTCGCT 
GCTGGAGAGC CGGCCATCCT GGAGTGTGTC CCTCCCAGAG GACACCCGGA GCCCACCATC 
TACTGGAAGA AAGACAAAGT CCGAATTGAT GAGAAGGATG ACAGGATCAA GATCCGTGGA 
GGGAAGCTGA TGATCTCTAA CACCAGGAAA AGCGACGCTG GAATGTACAT CTGCGTGGGC 
ACCAACATGG TCGGCGAAAG AGACAGTGAA ACAGCCCAGG TCACTGTGTT CGAGAGGCCC 
ACGTTCCTGC GGCGTCCCAT TAACCAGGTG GTTCTGGAGG AGGAGGCTGT GGAATTTCGA 
TGTCAGGTGC AGGGAGACCC GCAGCCGTCC ATCCGCTGGA AAAAAGACGA CGTTGAAATC 
CCCCGCGGCA GGTACGATAT GAAATTTGAT AAAGATGACT TCACGCTGAG AATAAAGAAG 
GCAACAGCGA GTGATGAAGG AACCTTCACC TGTGTAGCGG AGAACCGAGT GGGCAAAATG 
GAGGCCTCCG CAACCCTCAC GGTCAGAGCT GCACCGCAGT TCGTGATCAG GCCGCGGGAT 
CAGATTGTGG CACAGGGACG CACCGCCAGC TTCCCCTGCG AGACTAAAGG GAACCCGCAG 
CCGGCAGTCT TCTGGCAGAA AGAAGGAAGT CAGAATCTCC TGTTTCCAAA TCAACCCCAG 
CAGCCCAACA GTCGTTTCTC GGTGTCGCCC AGTGGAGATC TAACCATCAC TACGGTCCAG 
CGGGCAGATG CGGGCTATTA TATCTGCCAG GCCCTGACGG TGGCGGGCAG CATCCTGGCC 
AAAGCACAGC TTGAGGTCAC AGACGTGTTG ACCGATCGCC CTCCACCCAT CATCCGGCAG 
GGTCCAGCTA ACCAGACTCT TGGTGTAGAC AGTGTGGCTC TTTTGAGGTG CCATGCATCC 
GGAGAACCAG TCCCAACCAT CAGCTGGCTG AAGGACGGGG TCAGTCTGCT GGGCAAAGAC 
CCTCGGATGT CCCTGCAAGA CCTCGGCAGC CTGCAGATCA AGGGTTTACG GCTCTCTGAT 
TCTGGCATTT ATACTTGTGT GGCTGCAAGT TCTAGTGGGG AAACATCATG GAGTGCTTTC 
TTGGAGGTCA AAGAGGCTGG GAGTCTTGTT GTAGTTAAAG ATCGGGATGA GAACGAGCTT 
CCAGGACCTC CTTCCAAACC TCAGGTCACA GATGTCACTA AAAACAGCGT GTCATTATCC 
TGGCAGCCAG GCCTTCCCGG AGCATCAGCC ATTTCTACAT ATGTCATAGA AGCATTCAGC 
CAATCGGTGA GCAACAGCTG GCAAACTGTT GCCGATCACG CCAAGACAAC GCAGTACACC 
ATTAAGGGTC TCCGCCCTAA CACCATCTAC CTGTTCATGG TGCGAGCGGT CAACGTCCAA 
GGCCTGAGCG ACCCAAGCCC CATGTCTGAA CCCGTCCGCA CTCAAGATAT AAGTCCTCCA 
GCGCAGGGTG TGGATCACAG ACATGTGCAA AAGGAGCTGG GAGAAGTTAT TGTGCGGCTA 
CACAACCCTG TGGTCCTTAG CCCTACCACC ATACAGGTCA CATGGACGGT TGACCGCCAG 
TCTCAGTTCA TCCAGGGCTA CAGGGTTTTG TACAGGCAGA TGTCAGGCCT GTCTTCTCCA 
GGAGCCTGGC AAACTCAGGA TGTGAAAGTC CCCTCAGAGA GGAGCATGGT GCTCTCGGCT 
CTAAAGAAAG GCATCGTCTA CGAGATCAAA GTGCGTCCAT ATTTCAATGA GTTTCAGGGC 
ATGGACAGCG AATCCCGAAC CGCAAGAACA ACAGAGGAAG CGCCCAGTGC TCCTCCTCAG 
CAGGTGACTG TACTCACTGT GGGCAACCAG AACAGCACAT CTATTAGCAT CTCCTGGGAC 
CCTCCGCCTG CAGAACACCA GAACGGCATC ATACAGGAGT ACAAGATCTG GTGTTTGGGG 
AATGAGACCC GTTTTCATGT GAATAAGACG GTGGACGCAG CGATCCGCTC AGTGGTGGTT 
GGGGGTCTCC AGGCGGGGGT CCAGTATCGG GTGGAGGTGG CCGCTAGCAC CAGCGCCGGG 
GTCGGAGTGA AGAGCGAACC ACAAACCATC ATCATTGGTA AAGACTTCCG CGATGTGATG 
ATTAGCAGGA ATAACAGCAT CACAGAGCAG ATCACGGATG TGGTGAAACA GCCGGCCTTC 
ATCGCGGGCA TTGGCGGGGC CTGCTGGGTC ATTCTGATGG GCTTCAGCAT CTGGCTCTAC 
TGGAGGAGGA AGAAGAGAAA GGGACTCAGC AATTACGCAG TGACGTTCCA GCGTGTTGAT 
GGAGGCTTGA TGAGCAACGG GAGTCGTCCT GGTCTGCTGA ACGCCAGTGA TTCGGGTTAC 
CCGTGGCTCG CCGACTCCTG GCCTCCCAGC CTGCCTGTCA ACAGCGGATC TGGAGGACCC 
AATGACCTCA GCTTTGGCCG TGGAGATGTG ATGCCCAGTG CTCAGGCTGA TAAGACGGGT 
ACCATGCTTT CGGATGGAGC CATTTACAGC AGCATCGACT TCACCACCAA AGGCGGATTC 
GGTAGCCCAA GCCCAGCCTC CCAGGCCACG CCCTATGCCA CCACACAGAT CCTCCACTCC 
AACAGCATCC ATGAGCTGGC AGTGGATCTT CCTGAAGCCC AGTGGAAGAC CTCCCTACAG 
GCCAAGCAGG AGATGGCCAG CCTGGGTTAC TCACTGCCCG ACAAAAACTC CTGCAATAAC 
AGTGGAAAAG GTGGTAAGAA GAAGAAGACC AAAGCAAACG TCAAGCCCAC CAAGATGAAT 
GGCTCCAACT GGGCGAACGT TCCACTTCCT CCTCCTCCCA TCCACCCTCT TCCTGGCACA 
GAGATGGACC ACTACGCCTC AGAGCACCAT GATGGAGGAG GATATGATAG TGATGGTTGG 
GGCCCACCAT TGCCAGTGCA GACGTACCTC CATCAGGGTT TAGAGGATGA GCTGGAGGAA 
GAGGAAGAGC GGGTGCCAAC TCCTCCCATT AGGGGTGTGG CTTCTTCTCC TGCTGCAGTT 
TCCTTTGGCA AACAGTCCAC AGCCACACTG AGCCCATCTC CACGTGAAGA AATGCAGCCC 
ATGCTCCAAG CCCACCTAGA TGAGCTTACC CGTGCTTATC AATTGGACAT GGCTAAACAG 
ACTTGGCACA TGCAGGGTGG GCCTTACCCG CCCCAGGCCC CCGCACCACC CGTGGGCTAC 
GTCTCCAGCA CCTTAGTGTC TGACATCGAG ACCGATCTCC CTGATGAAGA TGACGATGAT 
GAGGAGGATG AAGGATACGA GATGTCGCCA CCAATTAGGG GCACTGAACA CACGCCAGGC 
TCCAGCATGG ACAACTTAGA CAGCTCCGTT ACGGGCTCTA TGGTGAACGG CTGGGGCTCG 
GCTTCAGAAG ATGATGACAG AAACTTCTCC AGCAGGAGGT CTAGTGCCGG CAGCTCTTCT 
GACGGCTCGC TCTTCACCCA TTGCAGCTTT GCGCGAGCCC TGGTGGCAGC CGCAGACAAG 
GCTGGTTACC GTCTGGATGG CACCAGCCTG AGCAAAAGAG ATAAAGGTCT ACCTCATAGA 
CCTCGTCCCG CTAGCCCGTT CTCCACAGAT GGAAGCACGG TGGGCACACA CAGTCTGGGG 
CACCAGCGGG CAGCAAGGCC CACACGTAAA CACAAAGGCC CTGGCACTGT CCCGAACCGC 
CGTGATGCCA GTGCGGATGA TCTGCCTCCA CCTCCAGAGC CTCCACCCTG CCAGACAGCT 
CGCATCCCCG GGTCGAGGAA CGTTCCCCAT CCCTGCGACC GCAAAGCTTC CTCTCTGGAG 
AGGCAGCCTA TGATGGGCCT TGAGGAAATG AGAAGTTCCT TGGACCGGCA GGCCCGAACC 
TCATTGGACC GCCACCAACA AGTCCAGGAC AGACTGGGGT CCATAGAGAG AGGAGATGAA 
AGCAGAAGAG GACAGAAAAA TGGTCCCTTT TCAGAAGAGG CCCTATTGCC ATACAACAAA 
CCATCATTCC CATCCCCTGG TGGGCACAGC TCATCTGGGA CGGCCTCTTC TAAAGGGTCA 
ACGGGTCCTC GCAAAGGAGA AGGGACGAGG GGACAGCACC AGCGCTCCAC CACGGAACAA 
GGAGAAAGCA GCTACATGGG GACCAACGCG CAGTTTTCCG GAGAGTTATG TGAGTCTGAA 
CAAGTTCAAG AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017206499.1
CDS	680..5314
Translation	MFLLSPVRLTLFLLVFLDVPHPSLQGRNKGRWNKGGSRLRQEDSPPRIVEHPSDLIVSKGEPATLNCKAEGRPTPTVEWYKDGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRRSKPDEGAYVCVARNYLGEAVSRNASLEVALLRDDFRQNPTDVVVAAGEPAILECVPPRGHPEPTIYWKKDKVRIDEKDDRIKIRGGKLMISNTRKSDAGMYICVGTNMVGERDSETAQVTVFERPTFLRRPINQVVLEEEAVEFRCQVQGDPQPSIRWKKDDVEIPRGRYDMKFDKDDFTLRIKKATASDEGTFTCVAENRVGKMEASATLTVRAAPQFVIRPRDQIVAQGRTASFPCETKGNPQPAVFWQKEGSQNLLFPNQPQQPNSRFSVSPSGDLTITTVQRADAGYYICQALTVAGSILAKAQLEVTDVLTDRPPPIIRQGPANQTLGVDSVALLRCHASGEPVPTISWLKDGVSLLGKDPRMSLQDLGSLQIKGLRLSDSGIYTCVAASSSGETSWSAFLEVKEAGSLVVVKDRDENELPGPPSKPQVTDVTKNSVSLSWQPGLPGASAISTYVIEAFSQSVSNSWQTVADHAKTTQYTIKGLRPNTIYLFMVRAVNVQGLSDPSPMSEPVRTQDISPPAQGVDHRHVQKELGEVIVRLHNPVVLSPTTIQVTWTVDRQSQFIQGYRVLYRQMSGLSSPGAWQTQDVKVPSERSMVLSALKKGIVYEIKVRPYFNEFQGMDSESRTARTTEEAPSAPPQQVTVLTVGNQNSTSISISWDPPPAEHQNGIIQEYKIWCLGNETRFHVNKTVDAAIRSVVVGGLQAGVQYRVEVAASTSAGVGVKSEPQTIIIGKDFRDVMISRNNSITEQITDVVKQPAFIAGIGGACWVILMGFSIWLYWRRKKRKGLSNYAVTFQRVDGGLMSNGSRPGLLNASDSGYPWLADSWPPSLPVNSGSGGPNDLSFGRGDVMPSAQADKTGTMLSDGAIYSSIDFTTKGGFGSPSPASQATPYATTQILHSNSIHELAVDLPEAQWKTSLQAKQEMASLGYSLPDKNSCNNSGKGGKKKKTKANVKPTKMNGSNWANVPLPPPPIHPLPGTEMDHYASEHHDGGGYDSDGWGPPLPVQTYLHQGLEDELEEEEERVPTPPIRGVASSPAAVSFGKQSTATLSPSPREEMQPMLQAHLDELTRAYQLDMAKQTWHMQGGPYPPQAPAPPVGYVSSTLVSDIETDLPDEDDDDEEDEGYEMSPPIRGTEHTPGSSMDNLDSSVTGSMVNGWGSASEDDDRNFSSRRSSAGSSSDGSLFTHCSFARALVAAADKAGYRLDGTSLSKRDKGLPHRPRPASPFSTDGSTVGTHSLGHQRAARPTRKHKGPGTVPNRRDASADDLPPPPEPPPCQTARIPGSRNVPHPCDRKASSLERQPMMGLEEMRSSLDRQARTSLDRHQQVQDRLGSIERGDESRRGQKNGPFSEEALLPYNKPSFPSPGGHSSSGTASSKGSTGPRKGEGTRGQHQRSTTEQGESSYMGTNAQFSGELCESEQVQE

Target ORF information:

RefSeq Version	XM_017351010.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio roundabout, axon guidance receptor, homolog 2 (Drosophila) (robo2), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017351010.1

ORF Insert Sequence:

ATGTTCCTCC TCTCTCCTGT GAGACTCACT TTATTTCTGT TGGTCTTCCT GGATGTTCCT 
CATCCGTCCC TTCAGGGCAG GAATAAGGGC AGATGGAACA AGGGGGGATC CCGTCTGCGG 
CAGGAAGATT CTCCACCTCG GATTGTAGAA CACCCCTCCG ATCTGATCGT GTCAAAGGGC 
GAGCCAGCCA CCCTAAACTG CAAGGCGGAG GGTCGGCCAA CTCCCACCGT GGAGTGGTAC 
AAGGATGGAG AACGAGTTGA AACGGACAAA GATGACCCTC GATCTCACCG CATGCTCCTT 
CCCAGTGGTT CGCTCTTCTT CCTGCGTATC GTACATGGAC GCCGCAGCAA ACCTGATGAG 
GGGGCGTACG TCTGTGTGGC ACGCAACTAC TTGGGGGAAG CAGTCAGTCG AAACGCCTCG 
CTGGAAGTAG CATTGTTACG GGATGATTTC CGGCAGAACC CTACAGATGT GGTGGTCGCT 
GCTGGAGAGC CGGCCATCCT GGAGTGTGTC CCTCCCAGAG GACACCCGGA GCCCACCATC 
TACTGGAAGA AAGACAAAGT CCGAATTGAT GAGAAGGATG ACAGGATCAA GATCCGTGGA 
GGGAAGCTGA TGATCTCTAA CACCAGGAAA AGCGACGCTG GAATGTACAT CTGCGTGGGC 
ACCAACATGG TCGGCGAAAG AGACAGTGAA ACAGCCCAGG TCACTGTGTT CGAGAGGCCC 
ACGTTCCTGC GGCGTCCCAT TAACCAGGTG GTTCTGGAGG AGGAGGCTGT GGAATTTCGA 
TGTCAGGTGC AGGGAGACCC GCAGCCGTCC ATCCGCTGGA AAAAAGACGA CGTTGAAATC 
CCCCGCGGCA GGTACGATAT GAAATTTGAT AAAGATGACT TCACGCTGAG AATAAAGAAG 
GCAACAGCGA GTGATGAAGG AACCTTCACC TGTGTAGCGG AGAACCGAGT GGGCAAAATG 
GAGGCCTCCG CAACCCTCAC GGTCAGAGCT GCACCGCAGT TCGTGATCAG GCCGCGGGAT 
CAGATTGTGG CACAGGGACG CACCGCCAGC TTCCCCTGCG AGACTAAAGG GAACCCGCAG 
CCGGCAGTCT TCTGGCAGAA AGAAGGAAGT CAGAATCTCC TGTTTCCAAA TCAACCCCAG 
CAGCCCAACA GTCGTTTCTC GGTGTCGCCC AGTGGAGATC TAACCATCAC TACGGTCCAG 
CGGGCAGATG CGGGCTATTA TATCTGCCAG GCCCTGACGG TGGCGGGCAG CATCCTGGCC 
AAAGCACAGC TTGAGGTCAC AGACGTGTTG ACCGATCGCC CTCCACCCAT CATCCGGCAG 
GGTCCAGCTA ACCAGACTCT TGGTGTAGAC AGTGTGGCTC TTTTGAGGTG CCATGCATCC 
GGAGAACCAG TCCCAACCAT CAGCTGGCTG AAGGACGGGG TCAGTCTGCT GGGCAAAGAC 
CCTCGGATGT CCCTGCAAGA CCTCGGCAGC CTGCAGATCA AGGGTTTACG GCTCTCTGAT 
TCTGGCATTT ATACTTGTGT GGCTGCAAGT TCTAGTGGGG AAACATCATG GAGTGCTTTC 
TTGGAGGTCA AAGAGGCTGG GAGTCTTGTT GTAGTTAAAG ATCGGGATGA GAACGAGCTT 
CCAGGACCTC CTTCCAAACC TCAGGTCACA GATGTCACTA AAAACAGCGT GTCATTATCC 
TGGCAGCCAG GCCTTCCCGG AGCATCAGCC ATTTCTACAT ATGTCATAGA AGCATTCAGC 
CAATCGGTGA GCAACAGCTG GCAAACTGTT GCCGATCACG CCAAGACAAC GCAGTACACC 
ATTAAGGGTC TCCGCCCTAA CACCATCTAC CTGTTCATGG TGCGAGCGGT CAACGTCCAA 
GGCCTGAGCG ACCCAAGCCC CATGTCTGAA CCCGTCCGCA CTCAAGATAT AAGTCCTCCA 
GCGCAGGGTG TGGATCACAG ACATGTGCAA AAGGAGCTGG GAGAAGTTAT TGTGCGGCTA 
CACAACCCTG TGGTCCTTAG CCCTACCACC ATACAGGTCA CATGGACGGT TGACCGCCAG 
TCTCAGTTCA TCCAGGGCTA CAGGGTTTTG TACAGGCAGA TGTCAGGCCT GTCTTCTCCA 
GGAGCCTGGC AAACTCAGGA TGTGAAAGTC CCCTCAGAGA GGAGCATGGT GCTCTCGGCT 
CTAAAGAAAG GCATCGTCTA CGAGATCAAA GTGCGTCCAT ATTTCAATGA GTTTCAGGGC 
ATGGACAGCG AATCCCGAAC CGCAAGAACA ACAGAGGAAG CGCCCAGTGC TCCTCCTCAG 
CAGGTGACTG TACTCACTGT GGGCAACCAG AACAGCACAT CTATTAGCAT CTCCTGGGAC 
CCTCCGCCTG CAGAACACCA GAACGGCATC ATACAGGAGT ACAAGATCTG GTGTTTGGGG 
AATGAGACCC GTTTTCATGT GAATAAGACG GTGGACGCAG CGATCCGCTC AGTGGTGGTT 
GGGGGTCTCC AGGCGGGGGT CCAGTATCGG GTGGAGGTGG CCGCTAGCAC CAGCGCCGGG 
GTCGGAGTGA AGAGCGAACC ACAAACCATC ATCATTGGTA AAGACTTCCG CGATGTGATG 
ATTAGCAGGA ATAACAGCAT CACAGAGCAG ATCACGGATG TGGTGAAACA GCCGGCCTTC 
ATCGCGGGCA TTGGCGGGGC CTGCTGGGTC ATTCTGATGG GCTTCAGCAT CTGGCTCTAC 
TGGAGGAGGA AGAAGAGAAA GGGACTCAGC AATTACGCAG TGACGTTCCA GCGTGTTGAT 
GGAGGCTTGA TGAGCAACGG GAGTCGTCCT GGTCTGCTGA ACGCCAGTGA TTCGGGTTAC 
CCGTGGCTCG CCGACTCCTG GCCTCCCAGC CTGCCTGTCA ACAGCGGATC TGGAGGACCC 
AATGACCTCA GCTTTGGCCG TGGAGATGTG ATGCCCAGTG CTCAGGCTGA TAAGACGGGT 
ACCATGCTTT CGGATGGAGC CATTTACAGC AGCATCGACT TCACCACCAA AGGCGGATTC 
GGTAGCCCAA GCCCAGCCTC CCAGGCCACG CCCTATGCCA CCACACAGAT CCTCCACTCC 
AACAGCATCC ATGAGCTGGC AGTGGATCTT CCTGAAGCCC AGTGGAAGAC CTCCCTACAG 
GCCAAGCAGG AGATGGCCAG CCTGGGTTAC TCACTGCCCG ACAAAAACTC CTGCAATAAC 
AGTGGAAAAG GTGGTAAGAA GAAGAAGACC AAAGCAAACG TCAAGCCCAC CAAGATGAAT 
GGCTCCAACT GGGCGAACGT TCCACTTCCT CCTCCTCCCA TCCACCCTCT TCCTGGCACA 
GAGATGGACC ACTACGCCTC AGAGCACCAT GATGGAGGAG GATATGATAG TGATGGTTGG 
GGCCCACCAT TGCCAGTGCA GACGTACCTC CATCAGGGTT TAGAGGATGA GCTGGAGGAA 
GAGGAAGAGC GGGTGCCAAC TCCTCCCATT AGGGGTGTGG CTTCTTCTCC TGCTGCAGTT 
TCCTTTGGCA AACAGTCCAC AGCCACACTG AGCCCATCTC CACGTGAAGA AATGCAGCCC 
ATGCTCCAAG CCCACCTAGA TGAGCTTACC CGTGCTTATC AATTGGACAT GGCTAAACAG 
ACTTGGCACA TGCAGGGTGG GCCTTACCCG CCCCAGGCCC CCGCACCACC CGTGGGCTAC 
GTCTCCAGCA CCTTAGTGTC TGACATCGAG ACCGATCTCC CTGATGAAGA TGACGATGAT 
GAGGAGGATG AAGGATACGA GATGTCGCCA CCAATTAGGG GCACTGAACA CACGCCAGGC 
TCCAGCATGG ACAACTTAGA CAGCTCCGTT ACGGGCTCTA TGGTGAACGG CTGGGGCTCG 
GCTTCAGAAG ATGATGACAG AAACTTCTCC AGCAGGAGGT CTAGTGCCGG CAGCTCTTCT 
GACGGCTCGC TCTTCACCCA TTGCAGCTTT GCGCGAGCCC TGGTGGCAGC CGCAGACAAG 
GCTGGTTACC GTCTGGATGG CACCAGCCTG AGCAAAAGAG ATAAAGGTCT ACCTCATAGA 
CCTCGTCCCG CTAGCCCGTT CTCCACAGAT GGAAGCACGG TGGGCACACA CAGTCTGGGG 
CACCAGCGGG CAGCAAGGCC CACACGTAAA CACAAAGGCC CTGGCACTGT CCCGAACCGC 
CGTGATGCCA GTGCGGATGA TCTGCCTCCA CCTCCAGAGC CTCCACCCTG CCAGACAGCT 
CGCATCCCCG GGTCGAGGAA CGTTCCCCAT CCCTGCGACC GCAAAGCTTC CTCTCTGGAG 
AGGCAGCCTA TGATGGGCCT TGAGGAAATG AGAAGTTCCT TGGACCGGCA GGCCCGAACC 
TCATTGGACC GCCACCAACA AGTCCAGGAC AGACTGGGGT CCATAGAGAG AGGAGATGAA 
AGCAGAAGAG GACAGAAAAA TGGTCCCTTT TCAGAAGAGG CCCTATTGCC ATACAACAAA 
CCATCATTCC CATCCCCTGG TGGGCACAGC TCATCTGGGA CGGCCTCTTC TAAAGGGTCA 
ACGGGTCCTC GCAAAGGAGA AGGGACGAGG GGACAGCACC AGCGCTCCAC CACGGAACAA 
GGAGAAAGCA GCTACATGGG GACCAACGCG CAGTTTTCCG GAGAGTTATG TGAGTCTGAA 
CAAGTTCAAG AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

robo2 ( NM_131633.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_571708.1 Danio rerio roundabout, axon guidance receptor, homolog 2 (Drosophila) (robo2), mRNA.

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CloneID	ODa02796
Clone ID Related Accession (Same CDS sequence)	NM_131633.1
Accession Version	NM_131633.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4542bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-20
Organism	Danio rerio(zebrafish)
Product	roundabout homolog 2 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF337035.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF337035.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168447, SAMEA2168448 [ECO:0000348] ##Evidence-Data-END##

ATGGGTCCTT TAACACACCT TTTACTCTGT GGATTAGTTT ACTTTAACCA AGTGGATGGA 
TCCCGTCTGC GGCAGGAAGA TTCTCCACCT CGGATTGTAG AACACCCCTC CGATCTGATC 
GTGTCAAAGG GCGAGCCAGC CACCCTAAAC TGCAAGGCGG AGGGTCGGCC AACTCCCACC 
GTGGAGTGGT ACAAGGATGG AGAACGAGTT GAAACGGACA AAGATGACCC TCGATCCCAC 
CGCATGCTCC TTCCCAGTGG TTCGCTCTTC TTCCTGCGTA TCGTACATGG ACGCCGCAGC 
AAACCTGATG AGGGGGCGTA CGTCTGTGTG GCACGCAACT ACTTGGGGGA AGCAGTCAGT 
CGAAACGCCT CGCTGGAAGT AGCATTGTTA CGGGATGATT TCCGGCAGAA CCCTACAGAT 
GTGGTGGTCG CTGCTGGAGA GCCGGCCATC CTGGAGTGTG TCCCTCCCAG AGGACACCCG 
GAGCCCACCA TCTACTGGAA GAAAGACAAA GTCCGAATTG ATGAGAAGGA TGACAGGATC 
AAGATCCGTG GAGGGAAGCT GATGATCTCT AACACCAGGA AAAGCGACGC TGGAATGTAC 
ATCTGCGTGG GCACCAACAT GGTCGGCGAA AGAGACAGTG AAACAGCCCA GGTCACTGTG 
TTCGAGAGGC CCACGTTCCT GCGGCGTCCC ACTAACCAGG TGGTTCTGGA GGAGGAGGCT 
GTGGAATTTC GGTGTCAGGT GCAGGGAGAC CCGCAGCCGT CCATCCGCTG GAAAAAAGAC 
GACGTTGAAA TCCCCCGCGG GAGGTACGAT ATGAAATTTG ATAAAGATGA CTTCACGCTG 
AGAATAAAGA AGGCAACAGC GAGTGATGAA GGAACCTTCA CCTGTGTAGC GGAGAACCGA 
GTGGGCAAAA TGGAGGCCTC CGCAACCCTC ACGGTCAGAG CTCGCCCTGT CGCTGCACCG 
CAGTTCGTGA TCAGGCCGCG GGATCAGATT GTGGCACAGG GACGCACCGC CAGCTTCCCC 
TGCGAGACCA AAGGGAACCC GCAGCCGGCA GTCTTCTGGC AGAAAGAAGG AAGTCAGAAT 
CTCCTGTTTC CAAATCAACC CCAGCAGCCC AACAGTCGTT TCTCGGTGTC GCCTAGTGGA 
GATCTAACCA TCACGGCGGT CCAGCGGGCA GATGCGGGCT ATTATATCTG CCAGGCCCTG 
ACGGTGGCGG GCAGCATCCT GGCCAAAGCA CAGCTTGAGG TCACAGACGT GTTGACCGAT 
CGCCCTCCAC CCATCATCCG GCAGGGTCCA GCTAACCAGA CTCTTGGTGT AGACAGTGTG 
GCTCTTTTGA GGTGCCATGC ATCCGGAGAA CCAGTCCCAA CCATCAGCTG GCTGAAGGAC 
GGGGTCAGTC TGCTGGGCAA AGACCCTCGG ATGTCCCTGC AAGACCTCGG CAGCCTGCAG 
ATCAAGGGTT TACGGCTCTC TGATTCTGGC ATTTATACTT GTGTGGCTGC AAGTTCTAGT 
GGGGAAACAT CATGGAGTGC TTTCTTGGAG GTCAAAGAGG CTGGGAGTCT TGTTGTAGTT 
AAAGATCGGG ATGAGAACGA GCTTCCAGGA CCTCCTTCCA AACCTCAGGT CACAGATGTC 
ACTAAAAACA GCGTGTCATT ATCCTGGCAG CCAGGCCTTC CCGGAGCATC AGCCATTTCT 
ACATATGTCA TAGAAGCATT CAGCCAATCA GTGAGCAACA GCTGGCAGAC TGTTGCCGAT 
CACGTCAAAA CAACGCAGTA CACCATTAAG GGTCTCCGCC CTAACACCAT CTACCTGTTC 
ATGGTGCGAG CGGTCAACGT CCAAGGCCTG AGCGACCCAA GCCCCATGTC TGAACCCGTC 
CGCACTCAAG ATATAAGTCC TCCAGCGCAG GGTGGTGATC ACAGACATGT GCAGAAGGAG 
CTGGGAGAAG TTATTGTGCG GCTACACAAC CCTGTGGTCC TTAGCCCTAC CACCATACAG 
GTCACATGGA CGGTTGACCG CCAGTCTCAG TTCATCCAGG GCTACAGGGT TTTGTACAGG 
CAGATGTCAG GCCTGTCTTC TCCAGGAGCC TGGCAAACTC AGGATGTGAA AGTCCCCTCA 
GAGAGGAGCA TGGTGCTCTC GGCTCTAAAG AAAGGCATCG TCTACGAGAT CAAAGTGCGT 
CCATATTTCA ATGAGTTTCA GGGCATGGAC AGCGAATCCC GAACCGCAAG AACAACAGAG 
GAAGCGCCCA GTGCTCCTCC TCAGCAGGTG ACTGTACTCA CGGTGGGCAA CCAGAACAGC 
ACATCTATTA GCATCTCCTG GGACCCTCCG CCTGCAGAAC ACCAGAACGG CATCATACAG 
GAGTACAAGA TCTGGTGTTT GGGGAATGAG ACCCGTTTTC ATGTGAATAA GACGGTGGAC 
GCAGCGATCC GCTCAGTGGT GGTTGGGGGT CTCCAGGCGG GGGTCCAGTA TCGGGTGGAG 
GTGGCCGCTA GCACCAGCGC CGGGGTCGGA GTGAAGAGCG AACCACAAAC CATCATCATT 
GGTAAAGACT TCCGCGATGT GATGATTAGC AGGAATAACA GCATCACAGA GCAGATCACG 
GATGTGGTGA AACAGCCGGC CTTCATCGCG GGCATTGGCG GGGCCTGCTG GGTCATTCTG 
ATGGGCTTCA GCATCTGGCT CTACTGGAGG AGGAAGAAGA GAAAGGGACT CAGCAATTAC 
GCAGTTCAGT CGTTCACCTT CACCCCTGCA GTGACGTTCC AGCGTGTTGA TGGAGGCTTG 
ATGAGCAACG GGAGTCGTCC TGGTCTGCTG AACGCCAGTG ATTCGGGTTA CCCGTGGCTC 
GCCGACTCCT GGCCGCCCAG CCTGCCTGTC AACAGCGGAT CTGGAGGACC CAATGACCTC 
AGCTTTGGCC GTGGAGATGT GATGCCCAGT GCTCAGGCTG ATAAGACGGG TACCATGCTT 
TCGGATGGAG CCATTTACAG CAGCATCGAC TTCACCACCA AAGGCGGATT CGGTAGCCCA 
AGCCCAGCCT CCCAGGCCAC GCCCTATGCC ACCACACAGA TCCTCCACTC CAACAGCATA 
CATGAGCTAG CAGTGGATCT TCCTGAAGCC CAGTGGAAGA CCTCCCTACA GGCCAAGCAG 
GAGATGGCTA GCCTGGGTTA CTCACTGCCC GACAAAAACT CCTGCAATAA CACACTCCTT 
TTCATTCCTG ACTACCGATT GGCTGACGGA CTGTCTAATA GAATGCCACA CAACCAATCG 
CAGGATTTCA GCACCACCAG CTCCCACAAC AGCTCAGATC GGAGCAACAG TCTGTCAGGT 
GGAAAAGGTG GTAAGAAGAA GAAGACCAAA GCAAACGTCA AGCCCACCAA GATGAATGGC 
TCCAACTGGG CGAACGTTCC ACTTCCTCCT CCTCCCATCC ACCCTCTTCC AGGCACAGAG 
ATGGACCACT ACGCCTCAGA GCACCATGAT GGAGGAGGAT ATGATAGTGA TGGTTGGGGC 
CCACCATTGC CAGTGCAGAC ATACCTCCAT CAGGGTTTAG AGGATGAGCT GGAGGAAGAG 
GAAGAGCGGG TGCCAACTCC TCCCATTAGG GGTGTGGCTT CTTCTCCTGC TGCAGTTTCC 
TTTGGCAAAC AGTCCACAGC CACACTGAGC CCATCTCCAC GTGAAGAAAT GCAGCCCATG 
CTCCAAGCCC ACCTAGATGA GCTTACCCGT GCTTATCAAT TGGACATGGC TAAACAGACT 
TGGCACATGC AGGGTGGGCC TTACCCGCCC CAGGCCCCCG CACCACCCGT GGGCTACATC 
TCCAGCACCT TAGTGTCTGA CATCGAGACC GATCTCCCTG ATGAAGATGA CGATGATGAG 
GAGGATGAAG GATACGAGAT GTCGCCACCA ATTAGGGGCA CTGAACACAC GCCAGGCTCC 
AGCATGGACA ACTTAGACAG CTCCGTTACG GATAAAGGTC TTCCTCATAG ACCTCGTCCC 
GCTAGCCCGT TCTCCACAGA TGGAAGCACG GTGGGCACAC ACAGTCTGGG GCACCAGCGG 
GCAGCAAGGC CCACACGGAA ACACAAAGGC CCTGGCACTG TCCCGAACCG CCGTGATGCC 
AGTGCGGATG ATCTGCCTCC ACCTCCAGAG CCTCCACCCT GCCAGACAGC TCGCATCCCC 
GGGTCGAGGA ACGTTCCCCA TCCCTGCGAC CGCAAAGCTT CCTCTCTGGA GAGGCAGCCT 
ATGATGGGCC TTGAGGAAAT GAGAAGTTCC TTGGACCGGC AGGCCCGAAC CTCATTGGAC 
CGCCACCAAC AAGTCCAGGA CAGACTGGGG TCCATAGAGA GAGGAGATGA AAGCAGAAGA 
GGACAGAAAA ATGGTCCCTT TTCAGAAGAG GCCCTATTGC CATACAACAA ACCATCATTC 
CCATCCCCTG GTGGGCACAG CTCATCTGGG ACGGCCTCTT CTAAAGGGTC AACGGGTCCT 
CGCAAAGGAG AAGGGACGAG GGGACAGCAC CAGCGCTCCA CCACGGAACA AGGTGAAAGC 
AGCTACATGG GGACCAACGC GCAGTTTTCC GGAGAGTTAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_571708.1
CDS	168..4709
Translation	MGPLTHLLLCGLVYFNQVDGSRLRQEDSPPRIVEHPSDLIVSKGEPATLNCKAEGRPTPTVEWYKDGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRRSKPDEGAYVCVARNYLGEAVSRNASLEVALLRDDFRQNPTDVVVAAGEPAILECVPPRGHPEPTIYWKKDKVRIDEKDDRIKIRGGKLMISNTRKSDAGMYICVGTNMVGERDSETAQVTVFERPTFLRRPTNQVVLEEEAVEFRCQVQGDPQPSIRWKKDDVEIPRGRYDMKFDKDDFTLRIKKATASDEGTFTCVAENRVGKMEASATLTVRARPVAAPQFVIRPRDQIVAQGRTASFPCETKGNPQPAVFWQKEGSQNLLFPNQPQQPNSRFSVSPSGDLTITAVQRADAGYYICQALTVAGSILAKAQLEVTDVLTDRPPPIIRQGPANQTLGVDSVALLRCHASGEPVPTISWLKDGVSLLGKDPRMSLQDLGSLQIKGLRLSDSGIYTCVAASSSGETSWSAFLEVKEAGSLVVVKDRDENELPGPPSKPQVTDVTKNSVSLSWQPGLPGASAISTYVIEAFSQSVSNSWQTVADHVKTTQYTIKGLRPNTIYLFMVRAVNVQGLSDPSPMSEPVRTQDISPPAQGGDHRHVQKELGEVIVRLHNPVVLSPTTIQVTWTVDRQSQFIQGYRVLYRQMSGLSSPGAWQTQDVKVPSERSMVLSALKKGIVYEIKVRPYFNEFQGMDSESRTARTTEEAPSAPPQQVTVLTVGNQNSTSISISWDPPPAEHQNGIIQEYKIWCLGNETRFHVNKTVDAAIRSVVVGGLQAGVQYRVEVAASTSAGVGVKSEPQTIIIGKDFRDVMISRNNSITEQITDVVKQPAFIAGIGGACWVILMGFSIWLYWRRKKRKGLSNYAVQSFTFTPAVTFQRVDGGLMSNGSRPGLLNASDSGYPWLADSWPPSLPVNSGSGGPNDLSFGRGDVMPSAQADKTGTMLSDGAIYSSIDFTTKGGFGSPSPASQATPYATTQILHSNSIHELAVDLPEAQWKTSLQAKQEMASLGYSLPDKNSCNNTLLFIPDYRLADGLSNRMPHNQSQDFSTTSSHNSSDRSNSLSGGKGGKKKKTKANVKPTKMNGSNWANVPLPPPPIHPLPGTEMDHYASEHHDGGGYDSDGWGPPLPVQTYLHQGLEDELEEEEERVPTPPIRGVASSPAAVSFGKQSTATLSPSPREEMQPMLQAHLDELTRAYQLDMAKQTWHMQGGPYPPQAPAPPVGYISSTLVSDIETDLPDEDDDDEEDEGYEMSPPIRGTEHTPGSSMDNLDSSVTDKGLPHRPRPASPFSTDGSTVGTHSLGHQRAARPTRKHKGPGTVPNRRDASADDLPPPPEPPPCQTARIPGSRNVPHPCDRKASSLERQPMMGLEEMRSSLDRQARTSLDRHQQVQDRLGSIERGDESRRGQKNGPFSEEALLPYNKPSFPSPGGHSSSGTASSKGSTGPRKGEGTRGQHQRSTTEQGESSYMGTNAQFSGEL

Target ORF information:

RefSeq Version	NM_131633.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio roundabout, axon guidance receptor, homolog 2 (Drosophila) (robo2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_131633.1

ORF Insert Sequence:

ATGGGTCCTT TAACACACCT TTTACTCTGT GGATTAGTTT ACTTTAACCA AGTGGATGGA 
TCCCGTCTGC GGCAGGAAGA TTCTCCACCT CGGATTGTAG AACACCCCTC CGATCTGATC 
GTGTCAAAGG GCGAGCCAGC CACCCTAAAC TGCAAGGCGG AGGGTCGGCC AACTCCCACC 
GTGGAGTGGT ACAAGGATGG AGAACGAGTT GAAACGGACA AAGATGACCC TCGATCCCAC 
CGCATGCTCC TTCCCAGTGG TTCGCTCTTC TTCCTGCGTA TCGTACATGG ACGCCGCAGC 
AAACCTGATG AGGGGGCGTA CGTCTGTGTG GCACGCAACT ACTTGGGGGA AGCAGTCAGT 
CGAAACGCCT CGCTGGAAGT AGCATTGTTA CGGGATGATT TCCGGCAGAA CCCTACAGAT 
GTGGTGGTCG CTGCTGGAGA GCCGGCCATC CTGGAGTGTG TCCCTCCCAG AGGACACCCG 
GAGCCCACCA TCTACTGGAA GAAAGACAAA GTCCGAATTG ATGAGAAGGA TGACAGGATC 
AAGATCCGTG GAGGGAAGCT GATGATCTCT AACACCAGGA AAAGCGACGC TGGAATGTAC 
ATCTGCGTGG GCACCAACAT GGTCGGCGAA AGAGACAGTG AAACAGCCCA GGTCACTGTG 
TTCGAGAGGC CCACGTTCCT GCGGCGTCCC ACTAACCAGG TGGTTCTGGA GGAGGAGGCT 
GTGGAATTTC GGTGTCAGGT GCAGGGAGAC CCGCAGCCGT CCATCCGCTG GAAAAAAGAC 
GACGTTGAAA TCCCCCGCGG GAGGTACGAT ATGAAATTTG ATAAAGATGA CTTCACGCTG 
AGAATAAAGA AGGCAACAGC GAGTGATGAA GGAACCTTCA CCTGTGTAGC GGAGAACCGA 
GTGGGCAAAA TGGAGGCCTC CGCAACCCTC ACGGTCAGAG CTCGCCCTGT CGCTGCACCG 
CAGTTCGTGA TCAGGCCGCG GGATCAGATT GTGGCACAGG GACGCACCGC CAGCTTCCCC 
TGCGAGACCA AAGGGAACCC GCAGCCGGCA GTCTTCTGGC AGAAAGAAGG AAGTCAGAAT 
CTCCTGTTTC CAAATCAACC CCAGCAGCCC AACAGTCGTT TCTCGGTGTC GCCTAGTGGA 
GATCTAACCA TCACGGCGGT CCAGCGGGCA GATGCGGGCT ATTATATCTG CCAGGCCCTG 
ACGGTGGCGG GCAGCATCCT GGCCAAAGCA CAGCTTGAGG TCACAGACGT GTTGACCGAT 
CGCCCTCCAC CCATCATCCG GCAGGGTCCA GCTAACCAGA CTCTTGGTGT AGACAGTGTG 
GCTCTTTTGA GGTGCCATGC ATCCGGAGAA CCAGTCCCAA CCATCAGCTG GCTGAAGGAC 
GGGGTCAGTC TGCTGGGCAA AGACCCTCGG ATGTCCCTGC AAGACCTCGG CAGCCTGCAG 
ATCAAGGGTT TACGGCTCTC TGATTCTGGC ATTTATACTT GTGTGGCTGC AAGTTCTAGT 
GGGGAAACAT CATGGAGTGC TTTCTTGGAG GTCAAAGAGG CTGGGAGTCT TGTTGTAGTT 
AAAGATCGGG ATGAGAACGA GCTTCCAGGA CCTCCTTCCA AACCTCAGGT CACAGATGTC 
ACTAAAAACA GCGTGTCATT ATCCTGGCAG CCAGGCCTTC CCGGAGCATC AGCCATTTCT 
ACATATGTCA TAGAAGCATT CAGCCAATCA GTGAGCAACA GCTGGCAGAC TGTTGCCGAT 
CACGTCAAAA CAACGCAGTA CACCATTAAG GGTCTCCGCC CTAACACCAT CTACCTGTTC 
ATGGTGCGAG CGGTCAACGT CCAAGGCCTG AGCGACCCAA GCCCCATGTC TGAACCCGTC 
CGCACTCAAG ATATAAGTCC TCCAGCGCAG GGTGGTGATC ACAGACATGT GCAGAAGGAG 
CTGGGAGAAG TTATTGTGCG GCTACACAAC CCTGTGGTCC TTAGCCCTAC CACCATACAG 
GTCACATGGA CGGTTGACCG CCAGTCTCAG TTCATCCAGG GCTACAGGGT TTTGTACAGG 
CAGATGTCAG GCCTGTCTTC TCCAGGAGCC TGGCAAACTC AGGATGTGAA AGTCCCCTCA 
GAGAGGAGCA TGGTGCTCTC GGCTCTAAAG AAAGGCATCG TCTACGAGAT CAAAGTGCGT 
CCATATTTCA ATGAGTTTCA GGGCATGGAC AGCGAATCCC GAACCGCAAG AACAACAGAG 
GAAGCGCCCA GTGCTCCTCC TCAGCAGGTG ACTGTACTCA CGGTGGGCAA CCAGAACAGC 
ACATCTATTA GCATCTCCTG GGACCCTCCG CCTGCAGAAC ACCAGAACGG CATCATACAG 
GAGTACAAGA TCTGGTGTTT GGGGAATGAG ACCCGTTTTC ATGTGAATAA GACGGTGGAC 
GCAGCGATCC GCTCAGTGGT GGTTGGGGGT CTCCAGGCGG GGGTCCAGTA TCGGGTGGAG 
GTGGCCGCTA GCACCAGCGC CGGGGTCGGA GTGAAGAGCG AACCACAAAC CATCATCATT 
GGTAAAGACT TCCGCGATGT GATGATTAGC AGGAATAACA GCATCACAGA GCAGATCACG 
GATGTGGTGA AACAGCCGGC CTTCATCGCG GGCATTGGCG GGGCCTGCTG GGTCATTCTG 
ATGGGCTTCA GCATCTGGCT CTACTGGAGG AGGAAGAAGA GAAAGGGACT CAGCAATTAC 
GCAGTTCAGT CGTTCACCTT CACCCCTGCA GTGACGTTCC AGCGTGTTGA TGGAGGCTTG 
ATGAGCAACG GGAGTCGTCC TGGTCTGCTG AACGCCAGTG ATTCGGGTTA CCCGTGGCTC 
GCCGACTCCT GGCCGCCCAG CCTGCCTGTC AACAGCGGAT CTGGAGGACC CAATGACCTC 
AGCTTTGGCC GTGGAGATGT GATGCCCAGT GCTCAGGCTG ATAAGACGGG TACCATGCTT 
TCGGATGGAG CCATTTACAG CAGCATCGAC TTCACCACCA AAGGCGGATT CGGTAGCCCA 
AGCCCAGCCT CCCAGGCCAC GCCCTATGCC ACCACACAGA TCCTCCACTC CAACAGCATA 
CATGAGCTAG CAGTGGATCT TCCTGAAGCC CAGTGGAAGA CCTCCCTACA GGCCAAGCAG 
GAGATGGCTA GCCTGGGTTA CTCACTGCCC GACAAAAACT CCTGCAATAA CACACTCCTT 
TTCATTCCTG ACTACCGATT GGCTGACGGA CTGTCTAATA GAATGCCACA CAACCAATCG 
CAGGATTTCA GCACCACCAG CTCCCACAAC AGCTCAGATC GGAGCAACAG TCTGTCAGGT 
GGAAAAGGTG GTAAGAAGAA GAAGACCAAA GCAAACGTCA AGCCCACCAA GATGAATGGC 
TCCAACTGGG CGAACGTTCC ACTTCCTCCT CCTCCCATCC ACCCTCTTCC AGGCACAGAG 
ATGGACCACT ACGCCTCAGA GCACCATGAT GGAGGAGGAT ATGATAGTGA TGGTTGGGGC 
CCACCATTGC CAGTGCAGAC ATACCTCCAT CAGGGTTTAG AGGATGAGCT GGAGGAAGAG 
GAAGAGCGGG TGCCAACTCC TCCCATTAGG GGTGTGGCTT CTTCTCCTGC TGCAGTTTCC 
TTTGGCAAAC AGTCCACAGC CACACTGAGC CCATCTCCAC GTGAAGAAAT GCAGCCCATG 
CTCCAAGCCC ACCTAGATGA GCTTACCCGT GCTTATCAAT TGGACATGGC TAAACAGACT 
TGGCACATGC AGGGTGGGCC TTACCCGCCC CAGGCCCCCG CACCACCCGT GGGCTACATC 
TCCAGCACCT TAGTGTCTGA CATCGAGACC GATCTCCCTG ATGAAGATGA CGATGATGAG 
GAGGATGAAG GATACGAGAT GTCGCCACCA ATTAGGGGCA CTGAACACAC GCCAGGCTCC 
AGCATGGACA ACTTAGACAG CTCCGTTACG GATAAAGGTC TTCCTCATAG ACCTCGTCCC 
GCTAGCCCGT TCTCCACAGA TGGAAGCACG GTGGGCACAC ACAGTCTGGG GCACCAGCGG 
GCAGCAAGGC CCACACGGAA ACACAAAGGC CCTGGCACTG TCCCGAACCG CCGTGATGCC 
AGTGCGGATG ATCTGCCTCC ACCTCCAGAG CCTCCACCCT GCCAGACAGC TCGCATCCCC 
GGGTCGAGGA ACGTTCCCCA TCCCTGCGAC CGCAAAGCTT CCTCTCTGGA GAGGCAGCCT 
ATGATGGGCC TTGAGGAAAT GAGAAGTTCC TTGGACCGGC AGGCCCGAAC CTCATTGGAC 
CGCCACCAAC AAGTCCAGGA CAGACTGGGG TCCATAGAGA GAGGAGATGA AAGCAGAAGA 
GGACAGAAAA ATGGTCCCTT TTCAGAAGAG GCCCTATTGC CATACAACAA ACCATCATTC 
CCATCCCCTG GTGGGCACAG CTCATCTGGG ACGGCCTCTT CTAAAGGGTC AACGGGTCCT 
CGCAAAGGAG AAGGGACGAG GGGACAGCAC CAGCGCTCCA CCACGGAACA AGGTGAAAGC 
AGCTACATGG GGACCAACGC GCAGTTTTCC GGAGAGTTAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	robo2
Entrez Gene ID	60309
Full Name	roundabout, axon guidance receptor, homolog 2 (Drosophila)
Synonyms	sb:eu937,unp1881
General protein information	Preferred Names roundabout, axon guidance receptor, homolog 2 (Drosophila) Names roundabout homolog 2 ast astray zmp:0000000549
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

Homo sapiens (human)	ROBO2	NP_002933.1
Rattus norvegicus (Norway rat)	Robo2	NP_115289.1
Canis lupus familiaris (dog)	LOC487691	XP_005638813.1
Bos taurus (cattle)	LOC534842	XP_002684698.3
Gallus gallus (chicken)	ROBO2	XP_416674.4
Danio rerio (zebrafish)	robo2	NP_571708.1
	LOC719197	XP_002802664.1
Mus musculus (house mouse)	Robo2	NP_780758.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010969	XP_309726.4
Drosophila melanogaster (fruit fly)	robo	NP_476899.1
Xenopus tropicalis (tropical clawed frog)	robo2	XP_004912137.1
Pan troglodytes (chimpanzee)	ROBO2	XP_516591.3

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

robo2 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

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