dclk2a cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	dclk2a
Entrez Gene ID	572548
Full Name	doublecortin-like kinase 2a
Synonyms	cb572,dclk,dclk2,sb:cb572,si:dkey-180n16.1
General protein information	Preferred Names doublecortin-like kinase 2a Names serine/threonine-protein kinase DCLK2 doublecortin-like kinase 2 zDCLK
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001145788.1	NP_001139260.1	serine/threonine-protein kinase DCLK2 isoform 3
NM_001135121.1	NP_001128593.1	serine/threonine-protein kinase DCLK2 isoform 1
XM_009292593.3	XP_009290868.1	serine/threonine-protein kinase DCLK2 isoform X1
NM_001145789.1	NP_001139261.1	serine/threonine-protein kinase DCLK2 isoform 4
XM_009292593.2	XP_009290868.1	serine/threonine-protein kinase DCLK2 isoform X1
NM_001145787.1	NP_001139259.1	serine/threonine-protein kinase DCLK2 isoform 2

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	DCLK2	XP_001150899.1
	DCLK2	XP_001082020.2
Canis lupus familiaris (dog)	DCLK2	XP_005629378.1
Bos taurus (cattle)	DCLK2	XP_005217546.1
Gallus gallus (chicken)	DCLK2	XP_003641209.1
Homo sapiens (human)	DCLK2	NP_001035351.4
Danio rerio (zebrafish)	dclk2	NP_001128593.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009034	XP_319785.4
Mus musculus (house mouse)	Dclk2	NP_001182425.1
Rattus norvegicus (Norway rat)	Dclk2	NP_001009691.3
Drosophila melanogaster (fruit fly)	CG17528	NP_001036463.1
Xenopus tropicalis (tropical clawed frog)	LOC100487296	XP_002933546.2

Gene Ontology
Bibliography

Related articles in PubMed

Adaptive optics improves multiphoton super-resolution imaging.
Zheng W, Wu Y, Winter P, Fischer R, Nogare DD, Hong A, McCormick C, Christensen R, Dempsey WP, Arnold DB, Zimmerberg J, Chitnis A, Sellers J, Waterman C, Shroff H
Nature methods14(9)869-872(2017 Sep)

Microtubule-actin crosslinking factor 1 (Macf1) domain function in Balbiani body dissociation and nuclear positioning.
Escobar-Aguirre M, Zhang H, Jamieson-Lucy A, Mullins MC
PLoS genetics13(9)e1006983(2017 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

the kinase fragment of DCLK is translocated into the nucleus upon hyperosmotic stresses and the kinase efficiently phosphorylates JDP2, a possible target in the nucleus, with the aid of histones
Title: Nuclear translocation of doublecortin-like protein kinase and phosphorylation of a transcription factor JDP2.

The following dclk2a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the dclk2a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa31127	NM_001135121.1 Latest version!	Danio rerio doublecortin-like kinase 2a (dclk2a), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
ODa34138	NM_001145787.1 Latest version!	Danio rerio doublecortin-like kinase 2a (dclk2a), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
ODa34164	NM_001145788.1 Latest version!	Danio rerio doublecortin-like kinase 2a (dclk2a), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
ODa36626	NM_001145789.1 Latest version!	Danio rerio doublecortin-like kinase 2a (dclk2a), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
ODa05912	XM_009292593.3 Latest version!	Danio rerio doublecortin-like kinase 2a (dclk2a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
View Old Accession Versions >>
ODa05912	XM_009292593.2	Danio rerio doublecortin-like kinase 2a (dclk2a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

dclk2a ( NM_001135121.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001128593.1 Danio rerio doublecortin-like kinase 2a (dclk2a), transcript variant 1, mRNA.

Price & Availability↑

CloneID	ODa31127
Clone ID Related Accession (Same CDS sequence)	NM_001135121.1
Accession Version	NM_001135121.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2433bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-22
Organism	Danio rerio(zebrafish)
Product	serine/threonine-protein kinase DCLK2 isoform 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AB266175.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB266175.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGTCCAGCA GAAACATCGA GTGGGAGCAC TTCGAGGAGC GCGAGAAGGG TCACCGGTCC 
CCGCGGGGCA GCGGCGGCTC CCATTCCGGC TCTCGGGGAA ACGGGATCGT GCCGAGTCCC 
GCTCACAGCG CGCACTGTAG CTTTTACCGC ACGCGCACAC TGCAGTCGCT CACCTCGGAG 
AAGAAAGCGA AAAAAGTTCG CTTTTATCGG AATGGCGACA AGTATTTCAA GGGGCTTGTG 
TACGCCGTGT CCGGTGACCG CTTCAGGTCT TTCGACGCGC TGCTGATGGA GCTCACGCGC 
TCCCTCTCGG ACAATGTCAA TTTACCGCAG GGAGTCCGGA GCATCTACAC TGCAGATGGA 
GGAAAGAAGA TCACCAGTCT GGATGACTTG GTAGAAGGAG AAAGCTACGT ATGTGCTTCC 
AACGAACCTT TTCGTAAAGT GGACTACACC AAGAATGTTA ATCCCAACTG GTCAGTCAAT 
GTGAAAACTG GTGCGTCCCG CTCCATGCCA TCACTTACCG CCACTAAAAA TGAGCTGAGA 
GAACGAGAGA GCAAAGACTA TATTAAGCCC AAGCTGGTGA CAGTCATCAG GAGTGGTGTT 
AAACCGCGTA AGGCTGTTCG AATTCTCCTG AACAAGAAAA CGGCTCACTC TTTTGAGCAG 
GTGCTCACAG ACATCACTGA CGCCATTAAA CTGGACTCCG GGGCCGTGAA GAGACTCTAC 
ACTCTGGAAG GCAAACAAAT TATATGTTTG CAGGATTTCT TTGGAGATGA TGACGTTTTC 
ATCGCGTGCG GCCCTGAGAA GTACCGTTAT GCACAGGATG ATTTTGTGTT GGATCACAGT 
GGTAAGGCTT CCACAGCCTT TATAACCGAA TGTCAGGTGA TCAAGTCCTC TTATTCTGCT 
CGCTCTGCTC CCCCTGTGAG ATACTCTGGA AGTAAAAGTC CAGGAACTGG CCGTCGCAGT 
AAATCCCCTG GATCTGCGAG GAGAACTGCA GGACACTTCT CCACAAATAG TCAGTCTCCA 
GTCAAATCAC CAATAAACGG TGCACCCAAC AGCCAAATCA CTACCCCCAA ATCCGCCAAA 
TCCTCCAGCT CGTCCCCGAC CAGCCCCCGC AGTATGCGCA ACTTCAAGAT TCCGACACAT 
CACTCCTCCG CAGGCAATGT GAACGGCTCC TCTGAGACGC CTCAGCATCA TAACAACAGC 
TTGAGCCCTG AAGTTAACGG AAACAGGAAC CTTGCTGCTT CCACTATTTT GGACAAGTAC 
AAAATTGGCA AAGTTATCGG TGATGGAAAC TTTGCTGTGG TGAAGGAATG TGTTGAACGA 
TCCACTGGAA AGGAGTTTGC ACTGAAGATC ATCGATAAAA ACAAATGCAG AGGCAAAGAG 
CACCTGATTG AGAATGAGGT GGCAGTGCTG AGGCGAGTTA AACATCCTAA CATCATCATG 
CTGATTGAGG AGGTGGATAC GCCTGCTGAG CTCTATCTGG TCATGGAACT TGTGAAGGGT 
GGAGATCTGT TTGATGCAAT CACCTCCTCA ACCAAATACA CTGAAAAAGA TGCCAGCGTG 
ATGGTGTTTG ACCTGGCAGC AGCTCTCAAA TATCTACACA GAATGTGCAT CGTTCATCGA 
GACATCAAAC CTGAAAATTT ACTGGTGTGT GAGTACCCTA ATGGTACAAA GTCTTTGAAG 
TTGGGGGATT TTGGCTTGGC GACAGTGGTT GAAGGGCCGC TGTACACTGT TTGTGGAACA 
CCGACTTATG TTGCCCCGGA AATCATAGCT GAGTCAGGCT ACGGGCTGAA AGTGGACATC 
TGGGCAGCTG GAGTGATTAC TTACATCCTA CTGTGTGGCT TCCCACCTTT CCGCAGTGAG 
AATAATCTAC AGGAGGACCT ATTTGATCAG ATCCTGCTGG GGCATTTAGA GTTCCCCTCA 
CCATTCTGGG ACAATATCTC TGATTCTGCT AAAGAGTTGA TTGGTCACAT GCTGCAGGTG 
AATGTGGAGG CCAGATACAC AGCGGAAGAT GTGCTCTCTC ACCCCTGGGT CACAGAGGAT 
GCAGCAATGG AAAACAACAT GAAGATGGAG GTGGCAGGTA AACTCAAAAA GCACTTTAAC 
TCTGTGCAGA AACAGAGCAA CACTTCTGCT GGAGTTTCGG TTATCATGAA CACAGCTCTA 
GATAAGGAAA CTATCCAGCT GTCCCACCGC CGCCAGGACC GCAACCCTCC TCGTCCAGAA 
AAGAGACCCT CCTCCACCAA CCCAGAAAGA CGACGTTCCA ACAGGGGACG CTCATCTGTC 
CAGTCGGAGG ACTCTAGAAG TGCTGCTCCC ATTGAGCCTG CAACCTCAGC AAACCCCAGT 
GCACCAGCAG CACTCCCTTC TGACCCTTCT CCGCAATTCC ATGAATGCAA TACTGAGTCT 
GAGGCCAGTA AAGACCTCCT GAAGGAAGAA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001128593.1
CDS	156..2588
Translation	MSSRNIEWEHFEEREKGHRSPRGSGGSHSGSRGNGIVPSPAHSAHCSFYRTRTLQSLTSEKKAKKVRFYRNGDKYFKGLVYAVSGDRFRSFDALLMELTRSLSDNVNLPQGVRSIYTADGGKKITSLDDLVEGESYVCASNEPFRKVDYTKNVNPNWSVNVKTGASRSMPSLTATKNELRERESKDYIKPKLVTVIRSGVKPRKAVRILLNKKTAHSFEQVLTDITDAIKLDSGAVKRLYTLEGKQIICLQDFFGDDDVFIACGPEKYRYAQDDFVLDHSGKASTAFITECQVIKSSYSARSAPPVRYSGSKSPGTGRRSKSPGSARRTAGHFSTNSQSPVKSPINGAPNSQITTPKSAKSSSSSPTSPRSMRNFKIPTHHSSAGNVNGSSETPQHHNNSLSPEVNGNRNLAASTILDKYKIGKVIGDGNFAVVKECVERSTGKEFALKIIDKNKCRGKEHLIENEVAVLRRVKHPNIIMLIEEVDTPAELYLVMELVKGGDLFDAITSSTKYTEKDASVMVFDLAAALKYLHRMCIVHRDIKPENLLVCEYPNGTKSLKLGDFGLATVVEGPLYTVCGTPTYVAPEIIAESGYGLKVDIWAAGVITYILLCGFPPFRSENNLQEDLFDQILLGHLEFPSPFWDNISDSAKELIGHMLQVNVEARYTAEDVLSHPWVTEDAAMENNMKMEVAGKLKKHFNSVQKQSNTSAGVSVIMNTALDKETIQLSHRRQDRNPPRPEKRPSSTNPERRRSNRGRSSVQSEDSRSAAPIEPATSANPSAPAALPSDPSPQFHECNTESEASKDLLKEE

Target ORF information:

RefSeq Version	NM_001135121.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio doublecortin-like kinase 2a (dclk2a), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001135121.1

ORF Insert Sequence:

ATGTCCAGCA GAAACATCGA GTGGGAGCAC TTCGAGGAGC GCGAGAAGGG TCACCGGTCC 
CCGCGGGGCA GCGGCGGCTC CCATTCCGGC TCTCGGGGAA ACGGGATCGT GCCGAGTCCC 
GCTCACAGCG CGCACTGTAG CTTTTACCGC ACGCGCACAC TGCAGTCGCT CACCTCGGAG 
AAGAAAGCGA AAAAAGTTCG CTTTTATCGG AATGGCGACA AGTATTTCAA GGGGCTTGTG 
TACGCCGTGT CCGGTGACCG CTTCAGGTCT TTCGACGCGC TGCTGATGGA GCTCACGCGC 
TCCCTCTCGG ACAATGTCAA TTTACCGCAG GGAGTCCGGA GCATCTACAC TGCAGATGGA 
GGAAAGAAGA TCACCAGTCT GGATGACTTG GTAGAAGGAG AAAGCTACGT ATGTGCTTCC 
AACGAACCTT TTCGTAAAGT GGACTACACC AAGAATGTTA ATCCCAACTG GTCAGTCAAT 
GTGAAAACTG GTGCGTCCCG CTCCATGCCA TCACTTACCG CCACTAAAAA TGAGCTGAGA 
GAACGAGAGA GCAAAGACTA TATTAAGCCC AAGCTGGTGA CAGTCATCAG GAGTGGTGTT 
AAACCGCGTA AGGCTGTTCG AATTCTCCTG AACAAGAAAA CGGCTCACTC TTTTGAGCAG 
GTGCTCACAG ACATCACTGA CGCCATTAAA CTGGACTCCG GGGCCGTGAA GAGACTCTAC 
ACTCTGGAAG GCAAACAAAT TATATGTTTG CAGGATTTCT TTGGAGATGA TGACGTTTTC 
ATCGCGTGCG GCCCTGAGAA GTACCGTTAT GCACAGGATG ATTTTGTGTT GGATCACAGT 
GGTAAGGCTT CCACAGCCTT TATAACCGAA TGTCAGGTGA TCAAGTCCTC TTATTCTGCT 
CGCTCTGCTC CCCCTGTGAG ATACTCTGGA AGTAAAAGTC CAGGAACTGG CCGTCGCAGT 
AAATCCCCTG GATCTGCGAG GAGAACTGCA GGACACTTCT CCACAAATAG TCAGTCTCCA 
GTCAAATCAC CAATAAACGG TGCACCCAAC AGCCAAATCA CTACCCCCAA ATCCGCCAAA 
TCCTCCAGCT CGTCCCCGAC CAGCCCCCGC AGTATGCGCA ACTTCAAGAT TCCGACACAT 
CACTCCTCCG CAGGCAATGT GAACGGCTCC TCTGAGACGC CTCAGCATCA TAACAACAGC 
TTGAGCCCTG AAGTTAACGG AAACAGGAAC CTTGCTGCTT CCACTATTTT GGACAAGTAC 
AAAATTGGCA AAGTTATCGG TGATGGAAAC TTTGCTGTGG TGAAGGAATG TGTTGAACGA 
TCCACTGGAA AGGAGTTTGC ACTGAAGATC ATCGATAAAA ACAAATGCAG AGGCAAAGAG 
CACCTGATTG AGAATGAGGT GGCAGTGCTG AGGCGAGTTA AACATCCTAA CATCATCATG 
CTGATTGAGG AGGTGGATAC GCCTGCTGAG CTCTATCTGG TCATGGAACT TGTGAAGGGT 
GGAGATCTGT TTGATGCAAT CACCTCCTCA ACCAAATACA CTGAAAAAGA TGCCAGCGTG 
ATGGTGTTTG ACCTGGCAGC AGCTCTCAAA TATCTACACA GAATGTGCAT CGTTCATCGA 
GACATCAAAC CTGAAAATTT ACTGGTGTGT GAGTACCCTA ATGGTACAAA GTCTTTGAAG 
TTGGGGGATT TTGGCTTGGC GACAGTGGTT GAAGGGCCGC TGTACACTGT TTGTGGAACA 
CCGACTTATG TTGCCCCGGA AATCATAGCT GAGTCAGGCT ACGGGCTGAA AGTGGACATC 
TGGGCAGCTG GAGTGATTAC TTACATCCTA CTGTGTGGCT TCCCACCTTT CCGCAGTGAG 
AATAATCTAC AGGAGGACCT ATTTGATCAG ATCCTGCTGG GGCATTTAGA GTTCCCCTCA 
CCATTCTGGG ACAATATCTC TGATTCTGCT AAAGAGTTGA TTGGTCACAT GCTGCAGGTG 
AATGTGGAGG CCAGATACAC AGCGGAAGAT GTGCTCTCTC ACCCCTGGGT CACAGAGGAT 
GCAGCAATGG AAAACAACAT GAAGATGGAG GTGGCAGGTA AACTCAAAAA GCACTTTAAC 
TCTGTGCAGA AACAGAGCAA CACTTCTGCT GGAGTTTCGG TTATCATGAA CACAGCTCTA 
GATAAGGAAA CTATCCAGCT GTCCCACCGC CGCCAGGACC GCAACCCTCC TCGTCCAGAA 
AAGAGACCCT CCTCCACCAA CCCAGAAAGA CGACGTTCCA ACAGGGGACG CTCATCTGTC 
CAGTCGGAGG ACTCTAGAAG TGCTGCTCCC ATTGAGCCTG CAACCTCAGC AAACCCCAGT 
GCACCAGCAG CACTCCCTTC TGACCCTTCT CCGCAATTCC ATGAATGCAA TACTGAGTCT 
GAGGCCAGTA AAGACCTCCT GAAGGAAGAA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dclk2a ( NM_001145787.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001139259.1 Danio rerio doublecortin-like kinase 2a (dclk2a), transcript variant 2, mRNA.

Price & Availability↑

CloneID	ODa34138
Clone ID Related Accession (Same CDS sequence)	NM_001145787.1
Accession Version	NM_001145787.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2376bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-21
Organism	Danio rerio(zebrafish)
Product	serine/threonine-protein kinase DCLK2 isoform 2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AB266176.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB266176.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGTCCAGCA GAAACATCGA GTGGGAGCAC TTCGAGGAGC GCGAGAAGGG TCACCGGTCC 
CCGCGGGGCA GCGGCGGCTC CCATTCCGGC TCTCGGGGAA ACGGGATCGT GCCGAGTCCC 
GCTCACAGCG CGCACTGTAG CTTTTACCGC ACGCGCACAC TGCAGTCGCT CACCTCGGAG 
AAGAAAGCAA AAAAAGTTCG CTTTTATCGG AATGGCGACA AGTATTTCAA GGGGCTTGTG 
TACGCCGTGT CCGGTGACCG CTTCAGGTCT TTCGACGCGC TGCTGATGGA GCTCACGCGC 
TCCCTCTCGG ACAATGTCAA TTTACCGCAG GGAGTCCGGA GCATCTACAC TGCAGATGGA 
GGAAAGAAGA TCACCAGTCT GGATGACTTG GTAGAAGGAG AAAGCTACGT ATGTGCTTCC 
AACGAACCTT TTCGTAAAGT GGACTACACC AAGAATGTTA ATCCCAACTG GTCAGTCAAT 
GTGAAAACTG GTGCGTCCCG CTCCATGCCA TCACTTACCG CCACTAAAAA TGAGCTGAGA 
GAACGAGAGA GCAAAGACTA CATTAAGCCT AAGCTGGTGA CAGTCATCAG GAGTGGTGTT 
AAACCGCGTA AGGCTGTTCG AATTCTCCTG AACAAGAAAA CGGCTCACTC TTTTGAGCAG 
GTGCTCACCG ACATCACTGA CGCCATTAAA CTGGACTCCG GGGCCGTGAA GAGACTCTAC 
ACTCTGGAAG GCAAACAAAT TATATGTTTG CAGGATTTCT TTGGAGATGA TGACGTTTTC 
ATCGCGTGCG GCCCTGAGAA GTACCGTTAT GCACAGGATG ATTTTGTGTT GGATCACAGT 
GGTAAGGCTT CCACAGCCTT TATAACCGAA TGTCAGGTGA TCAAGTCCTC TTATTCTGCT 
CGCTCTGCTC CCCCTGTGAG ATACTCTGGA AGTAAAAGTC CAGGAACTGG CCGTCGCAGT 
AAATCCCCTG GATCTGTAAA CGGTGCACCC AACAGCCAAA TCACTACCCC CAAATCCGCC 
AAATCCTCCA GCTCGTCCCC GACCAGCCCC CGCAGTATGC GCAACTTCAA GATTCCGACA 
CATCACTCCT CCGCAGGCAA TGTGAACGGC TCCTCTGAGA CGCCTCAGCA TCATAACAAC 
AGCTTGAGCC CTGAAGTTAA CGGAAACAGG AACCTTGCTG CTTCCACTAT TTTGGACAAG 
TACAAAATTG GCAAAGTTAT CGGTGATGGA AACTTTGCTG TGGTGAAGGA ATGTGTTGAA 
CGATCCACTG GAAAGGAGTT TGCACTGAAG ATCATCGATA AAAACAAATG CAGAGGCAAA 
GAGCACCTGA TTGAGAATGA GGTGGCAGTG CTGAGGCGAG TTAAACATCC TAACATCATC 
ATGCTGATTG AGGAGGTGGA TACGCCTGCT GAGCTCTATC TGGTCATGGA ACTTGTGAAG 
GGTGGAGATC TGTTTGATGC AATCACCTCC TCAACCAAAT ACACTGAAAA AGATGCCAGC 
GTGATGGTGT TTGACCTGGC AGCAGCTCTC AAATATCTAC ACAGAATGTG CATCGTTCAT 
CGAGACATCA AACCTGAAAA TCTACTGGTG TGTGAGTACC CTAATGGTAC AAAGTCTTTG 
AAGTTGGGGG ATTTTGGCTT GGCGACAGTG GTTGAAGGGC CGCTGTACAC TGTTTGTGGA 
ACACCGACTT ATGTTGCCCC GGAAATCATA GCTGAGTCAG GCTACGGGCT GAAAGTGGAC 
ATCTGGGCAG CTGGAGTGAT TACCTACATC CTACTGTGTG GCTTCCCACC TTTCCGCAGT 
GAGAATAATC TACAGGAGGA CCTATTTGAT CAGATCCTGC TGGGGCATTT AGAGTTCCCT 
TCACCATTCT GGGACAATAT CTCTGATTCT GCTAAAGAGT TGATTGGTCA CATGCTGCAG 
GTGAATGTGG AGGCCAGATA CACAGCGGAA GATGTGCTCT CTCACCCCTG GGTCACAGAG 
GATGCAGCAA TGGAAAACAA CATGAAGATG GAGGTGGCAG GTAAACTTAA AAAGCACTTT 
AACTCTGTGC AGAAACAGAG CAACACTTCT GCTGGAGTTT CGGTTATCAT GAACACAGCT 
CTAGATAAGG AAACTATCCA GCTGTCCCAC CGCCGCCAAG ACCGCAACCC TCCTCGTCCA 
GAAAAGAGAC CCTCCTCCAC CAACCCAGAA AGACGACGTT CCAACAGGGG ACGCTCATCT 
GTCCAGTCGG AGGACTCTAG AAGTGCTGCT CCCATTGAGC CTGCAACCTC AGCAAACCCC 
AGTGCACCAG CAGCACTCCC TTCTGACCCT TCTCCGCAAT TCCATGAATG CAATACTGAG 
TCTGAGGCCA GTAAAGACCT CCTGAAGGAA GAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001139259.1
CDS	156..2531
Translation	MSSRNIEWEHFEEREKGHRSPRGSGGSHSGSRGNGIVPSPAHSAHCSFYRTRTLQSLTSEKKAKKVRFYRNGDKYFKGLVYAVSGDRFRSFDALLMELTRSLSDNVNLPQGVRSIYTADGGKKITSLDDLVEGESYVCASNEPFRKVDYTKNVNPNWSVNVKTGASRSMPSLTATKNELRERESKDYIKPKLVTVIRSGVKPRKAVRILLNKKTAHSFEQVLTDITDAIKLDSGAVKRLYTLEGKQIICLQDFFGDDDVFIACGPEKYRYAQDDFVLDHSGKASTAFITECQVIKSSYSARSAPPVRYSGSKSPGTGRRSKSPGSVNGAPNSQITTPKSAKSSSSSPTSPRSMRNFKIPTHHSSAGNVNGSSETPQHHNNSLSPEVNGNRNLAASTILDKYKIGKVIGDGNFAVVKECVERSTGKEFALKIIDKNKCRGKEHLIENEVAVLRRVKHPNIIMLIEEVDTPAELYLVMELVKGGDLFDAITSSTKYTEKDASVMVFDLAAALKYLHRMCIVHRDIKPENLLVCEYPNGTKSLKLGDFGLATVVEGPLYTVCGTPTYVAPEIIAESGYGLKVDIWAAGVITYILLCGFPPFRSENNLQEDLFDQILLGHLEFPSPFWDNISDSAKELIGHMLQVNVEARYTAEDVLSHPWVTEDAAMENNMKMEVAGKLKKHFNSVQKQSNTSAGVSVIMNTALDKETIQLSHRRQDRNPPRPEKRPSSTNPERRRSNRGRSSVQSEDSRSAAPIEPATSANPSAPAALPSDPSPQFHECNTESEASKDLLKEE

Target ORF information:

RefSeq Version	NM_001145787.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio doublecortin-like kinase 2a (dclk2a), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001145787.1

ORF Insert Sequence:

ATGTCCAGCA GAAACATCGA GTGGGAGCAC TTCGAGGAGC GCGAGAAGGG TCACCGGTCC 
CCGCGGGGCA GCGGCGGCTC CCATTCCGGC TCTCGGGGAA ACGGGATCGT GCCGAGTCCC 
GCTCACAGCG CGCACTGTAG CTTTTACCGC ACGCGCACAC TGCAGTCGCT CACCTCGGAG 
AAGAAAGCAA AAAAAGTTCG CTTTTATCGG AATGGCGACA AGTATTTCAA GGGGCTTGTG 
TACGCCGTGT CCGGTGACCG CTTCAGGTCT TTCGACGCGC TGCTGATGGA GCTCACGCGC 
TCCCTCTCGG ACAATGTCAA TTTACCGCAG GGAGTCCGGA GCATCTACAC TGCAGATGGA 
GGAAAGAAGA TCACCAGTCT GGATGACTTG GTAGAAGGAG AAAGCTACGT ATGTGCTTCC 
AACGAACCTT TTCGTAAAGT GGACTACACC AAGAATGTTA ATCCCAACTG GTCAGTCAAT 
GTGAAAACTG GTGCGTCCCG CTCCATGCCA TCACTTACCG CCACTAAAAA TGAGCTGAGA 
GAACGAGAGA GCAAAGACTA CATTAAGCCT AAGCTGGTGA CAGTCATCAG GAGTGGTGTT 
AAACCGCGTA AGGCTGTTCG AATTCTCCTG AACAAGAAAA CGGCTCACTC TTTTGAGCAG 
GTGCTCACCG ACATCACTGA CGCCATTAAA CTGGACTCCG GGGCCGTGAA GAGACTCTAC 
ACTCTGGAAG GCAAACAAAT TATATGTTTG CAGGATTTCT TTGGAGATGA TGACGTTTTC 
ATCGCGTGCG GCCCTGAGAA GTACCGTTAT GCACAGGATG ATTTTGTGTT GGATCACAGT 
GGTAAGGCTT CCACAGCCTT TATAACCGAA TGTCAGGTGA TCAAGTCCTC TTATTCTGCT 
CGCTCTGCTC CCCCTGTGAG ATACTCTGGA AGTAAAAGTC CAGGAACTGG CCGTCGCAGT 
AAATCCCCTG GATCTGTAAA CGGTGCACCC AACAGCCAAA TCACTACCCC CAAATCCGCC 
AAATCCTCCA GCTCGTCCCC GACCAGCCCC CGCAGTATGC GCAACTTCAA GATTCCGACA 
CATCACTCCT CCGCAGGCAA TGTGAACGGC TCCTCTGAGA CGCCTCAGCA TCATAACAAC 
AGCTTGAGCC CTGAAGTTAA CGGAAACAGG AACCTTGCTG CTTCCACTAT TTTGGACAAG 
TACAAAATTG GCAAAGTTAT CGGTGATGGA AACTTTGCTG TGGTGAAGGA ATGTGTTGAA 
CGATCCACTG GAAAGGAGTT TGCACTGAAG ATCATCGATA AAAACAAATG CAGAGGCAAA 
GAGCACCTGA TTGAGAATGA GGTGGCAGTG CTGAGGCGAG TTAAACATCC TAACATCATC 
ATGCTGATTG AGGAGGTGGA TACGCCTGCT GAGCTCTATC TGGTCATGGA ACTTGTGAAG 
GGTGGAGATC TGTTTGATGC AATCACCTCC TCAACCAAAT ACACTGAAAA AGATGCCAGC 
GTGATGGTGT TTGACCTGGC AGCAGCTCTC AAATATCTAC ACAGAATGTG CATCGTTCAT 
CGAGACATCA AACCTGAAAA TCTACTGGTG TGTGAGTACC CTAATGGTAC AAAGTCTTTG 
AAGTTGGGGG ATTTTGGCTT GGCGACAGTG GTTGAAGGGC CGCTGTACAC TGTTTGTGGA 
ACACCGACTT ATGTTGCCCC GGAAATCATA GCTGAGTCAG GCTACGGGCT GAAAGTGGAC 
ATCTGGGCAG CTGGAGTGAT TACCTACATC CTACTGTGTG GCTTCCCACC TTTCCGCAGT 
GAGAATAATC TACAGGAGGA CCTATTTGAT CAGATCCTGC TGGGGCATTT AGAGTTCCCT 
TCACCATTCT GGGACAATAT CTCTGATTCT GCTAAAGAGT TGATTGGTCA CATGCTGCAG 
GTGAATGTGG AGGCCAGATA CACAGCGGAA GATGTGCTCT CTCACCCCTG GGTCACAGAG 
GATGCAGCAA TGGAAAACAA CATGAAGATG GAGGTGGCAG GTAAACTTAA AAAGCACTTT 
AACTCTGTGC AGAAACAGAG CAACACTTCT GCTGGAGTTT CGGTTATCAT GAACACAGCT 
CTAGATAAGG AAACTATCCA GCTGTCCCAC CGCCGCCAAG ACCGCAACCC TCCTCGTCCA 
GAAAAGAGAC CCTCCTCCAC CAACCCAGAA AGACGACGTT CCAACAGGGG ACGCTCATCT 
GTCCAGTCGG AGGACTCTAG AAGTGCTGCT CCCATTGAGC CTGCAACCTC AGCAAACCCC 
AGTGCACCAG CAGCACTCCC TTCTGACCCT TCTCCGCAAT TCCATGAATG CAATACTGAG 
TCTGAGGCCA GTAAAGACCT CCTGAAGGAA GAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dclk2a ( NM_001145788.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001139260.1 Danio rerio doublecortin-like kinase 2a (dclk2a), transcript variant 3, mRNA.

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CloneID	ODa34164
Clone ID Related Accession (Same CDS sequence)	NM_001145788.1
Accession Version	NM_001145788.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2178bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-21
Organism	Danio rerio(zebrafish)
Product	serine/threonine-protein kinase DCLK2 isoform 3
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AB266177.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB266177.1, BC163926.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGTCCAGCA GAAACATCGA GTGGGAGCAC TTCGAGGAGC GCGAGAAGGG TCACCGGTCC 
CCGCGGGGCA GCGGCGGCTC CCATTCCGGC TCTCGGGGAA ACGGGATCGT GCCGAGTCCC 
GCTCACAGCG CGCACTGTAG CTTTTACCGC ACGCGCACAC TGCAGTCGCT CACCTCGGAG 
AAGAAAGCGA AAAAAGTTCG CTTTTATCGG AATGGCGACA AGTATTTCAA GGGGCTTGTG 
TACGCCGTGT CCGGTGACCG CTTCAGGTCT TTCGACGCGC TGCTGATGGA GCTCACGCGC 
TCCCTCTCGG ACAATGTCAA TTTACCGCAG GGAGTCCGGA GCATCTACAC TGCAGATGGA 
GGAAAGAAGA TCACCAGTCT GGATGACTTG GTAGAAGGAG AAAGCTACGT ATGTGCTTCC 
AACGAACCTT TTCGTAAAGT GGACTACACC AAGAATGTTA ATCCCAACTG GTCAGTCAAT 
GTGAAAACTG GTGCGTCCCG CTCCATGCCA TCACTTACCG CCACTAAAAA TGAGCTGAGA 
GAACGAGAGA GCAAAGACTA TATTAAGCCC AAGCTGGTGA CAGTCATCAG GAGTGGTGTT 
AAACCGCGTA AGGCTGTTCG AATTCTCCTG AACAAGAAAA CGGCTCACTC TTTTGAGCAG 
GTGCTCACAG ACATCACTGA CGCCATTAAA CTGGACTCCG GGGCCGTGAA GAGACTCTAC 
ACTCTGGAAG GCAAACAAAT TATATGTTTG CAGGATTTCT TTGGAGATGA TGACGTTTTC 
ATCGCGTGCG GCCCTGAGAA GTACCGTTAT GCACAGGATG ATTTTGTGTT GGATCACAGT 
GGTAAGGCTT CCACAGCCTT TATAACCGAA TGTCAGGTGA TCAAGTCCTC TTATTCTGCT 
CGCTCTGCTC CCCCTGTGAG ATACTCTGGA AGTAAAAGTC CAGGAACTGG CCGTCGCAGT 
AAATCCCCTG GATCTGCGAG GAGAACTGCA GGACACTTCT CCACAAATAG TCAGTCTCCA 
GTCAAATCAC CAATAAACGG TGCACCCAAC AGCCAAATCA CTACCCCCAA ATCCGCCAAA 
TCCTCCAGCT CGTCCCCGAC CAGCCCCCGC AGTATGCGCA ACTTCAAGAT TCCGACACAT 
CACTCCTCCG CAGGCAATGT GAACGGCTCC TCTGAGACGC CTCAGCATCA TAACAACAGC 
TTGAGCCCTG AAGTTAACGG AAACAGGAAC CTTGCTGCTT CCACTATTTT GGACAAGTAC 
AAAATTGGCA AAGTTATCGG TGATGGAAAC TTTGCTGTGG TGAAGGAATG TGTTGAACGA 
TCCACTGGAA AGGAGTTTGC ACTGAAGATC ATCGATAAAA ACAAATGCAG AGGCAAAGAG 
CACCTGATTG AGAATGAGGT GGCAGTGCTG AGGCGAGTTA AACATCCTAA CATCATCATG 
CTGATTGAGG AGGTGGATAC GCCTGCTGAG CTCTATCTGG TCATGGAACT TGTGAAGGGT 
GGAGATCTGT TTGATGCAAT CACCTCCTCA ACCAAATACA CTGAAAAAGA TGCCAGCGTG 
ATGGTGTTTG ACCTGGCAGC AGCTCTCAAA TATCTACACA GAATGTGCAT CGTTCATCGA 
GACATCAAAC CTGAAAATTT ACTGGTGTGT GAGTACCCTA ATGGTACAAA GTCTTTGAAG 
TTGGGGGATT TTGGCTTGGC GACAGTGGTT GAAGGGCCGC TGTACACTGT TTGTGGAACA 
CCGACTTATG TTGCCCCGGA AATCATAGCT GAGTCAGGCT ACGGGCTGAA AGTGGACATC 
TGGGCAGCTG GAGTGATTAC TTACATCCTA CTGTGTGGCT TCCCACCTTT CCGCAGTGAG 
AATAATCTAC AGGAGGACCT ATTTGATCAG ATCCTGCTGG GGCATTTAGA GTTCCCCTCA 
CCATTCTGGG ACAATATCTC TGATTCTGCT AAAGAGTTGA TTGGTCACAT GCTGCAGGTG 
AATGTGGAGG CCAGATACAC AGCGGAAGAT GTGCTCTCTC ACCCCTGGGT CACAGAGGAT 
GCAGCAATGG AAAACAACAT GAAGATGGAG GTGGCAGGTA AACTCAAAAA GCACTTTAAC 
TCTGTGCAGA AACAGAGCAA CACTTCTGCT GGAGTTTCGG TTATCATGCT CCAAGGCAAT 
GAACACAGCT CTAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001139260.1
CDS	156..2333
Translation	MSSRNIEWEHFEEREKGHRSPRGSGGSHSGSRGNGIVPSPAHSAHCSFYRTRTLQSLTSEKKAKKVRFYRNGDKYFKGLVYAVSGDRFRSFDALLMELTRSLSDNVNLPQGVRSIYTADGGKKITSLDDLVEGESYVCASNEPFRKVDYTKNVNPNWSVNVKTGASRSMPSLTATKNELRERESKDYIKPKLVTVIRSGVKPRKAVRILLNKKTAHSFEQVLTDITDAIKLDSGAVKRLYTLEGKQIICLQDFFGDDDVFIACGPEKYRYAQDDFVLDHSGKASTAFITECQVIKSSYSARSAPPVRYSGSKSPGTGRRSKSPGSARRTAGHFSTNSQSPVKSPINGAPNSQITTPKSAKSSSSSPTSPRSMRNFKIPTHHSSAGNVNGSSETPQHHNNSLSPEVNGNRNLAASTILDKYKIGKVIGDGNFAVVKECVERSTGKEFALKIIDKNKCRGKEHLIENEVAVLRRVKHPNIIMLIEEVDTPAELYLVMELVKGGDLFDAITSSTKYTEKDASVMVFDLAAALKYLHRMCIVHRDIKPENLLVCEYPNGTKSLKLGDFGLATVVEGPLYTVCGTPTYVAPEIIAESGYGLKVDIWAAGVITYILLCGFPPFRSENNLQEDLFDQILLGHLEFPSPFWDNISDSAKELIGHMLQVNVEARYTAEDVLSHPWVTEDAAMENNMKMEVAGKLKKHFNSVQKQSNTSAGVSVIMLQGNEHSSR

Target ORF information:

RefSeq Version	NM_001145788.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio doublecortin-like kinase 2a (dclk2a), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001145788.1

ORF Insert Sequence:

ATGTCCAGCA GAAACATCGA GTGGGAGCAC TTCGAGGAGC GCGAGAAGGG TCACCGGTCC 
CCGCGGGGCA GCGGCGGCTC CCATTCCGGC TCTCGGGGAA ACGGGATCGT GCCGAGTCCC 
GCTCACAGCG CGCACTGTAG CTTTTACCGC ACGCGCACAC TGCAGTCGCT CACCTCGGAG 
AAGAAAGCGA AAAAAGTTCG CTTTTATCGG AATGGCGACA AGTATTTCAA GGGGCTTGTG 
TACGCCGTGT CCGGTGACCG CTTCAGGTCT TTCGACGCGC TGCTGATGGA GCTCACGCGC 
TCCCTCTCGG ACAATGTCAA TTTACCGCAG GGAGTCCGGA GCATCTACAC TGCAGATGGA 
GGAAAGAAGA TCACCAGTCT GGATGACTTG GTAGAAGGAG AAAGCTACGT ATGTGCTTCC 
AACGAACCTT TTCGTAAAGT GGACTACACC AAGAATGTTA ATCCCAACTG GTCAGTCAAT 
GTGAAAACTG GTGCGTCCCG CTCCATGCCA TCACTTACCG CCACTAAAAA TGAGCTGAGA 
GAACGAGAGA GCAAAGACTA TATTAAGCCC AAGCTGGTGA CAGTCATCAG GAGTGGTGTT 
AAACCGCGTA AGGCTGTTCG AATTCTCCTG AACAAGAAAA CGGCTCACTC TTTTGAGCAG 
GTGCTCACAG ACATCACTGA CGCCATTAAA CTGGACTCCG GGGCCGTGAA GAGACTCTAC 
ACTCTGGAAG GCAAACAAAT TATATGTTTG CAGGATTTCT TTGGAGATGA TGACGTTTTC 
ATCGCGTGCG GCCCTGAGAA GTACCGTTAT GCACAGGATG ATTTTGTGTT GGATCACAGT 
GGTAAGGCTT CCACAGCCTT TATAACCGAA TGTCAGGTGA TCAAGTCCTC TTATTCTGCT 
CGCTCTGCTC CCCCTGTGAG ATACTCTGGA AGTAAAAGTC CAGGAACTGG CCGTCGCAGT 
AAATCCCCTG GATCTGCGAG GAGAACTGCA GGACACTTCT CCACAAATAG TCAGTCTCCA 
GTCAAATCAC CAATAAACGG TGCACCCAAC AGCCAAATCA CTACCCCCAA ATCCGCCAAA 
TCCTCCAGCT CGTCCCCGAC CAGCCCCCGC AGTATGCGCA ACTTCAAGAT TCCGACACAT 
CACTCCTCCG CAGGCAATGT GAACGGCTCC TCTGAGACGC CTCAGCATCA TAACAACAGC 
TTGAGCCCTG AAGTTAACGG AAACAGGAAC CTTGCTGCTT CCACTATTTT GGACAAGTAC 
AAAATTGGCA AAGTTATCGG TGATGGAAAC TTTGCTGTGG TGAAGGAATG TGTTGAACGA 
TCCACTGGAA AGGAGTTTGC ACTGAAGATC ATCGATAAAA ACAAATGCAG AGGCAAAGAG 
CACCTGATTG AGAATGAGGT GGCAGTGCTG AGGCGAGTTA AACATCCTAA CATCATCATG 
CTGATTGAGG AGGTGGATAC GCCTGCTGAG CTCTATCTGG TCATGGAACT TGTGAAGGGT 
GGAGATCTGT TTGATGCAAT CACCTCCTCA ACCAAATACA CTGAAAAAGA TGCCAGCGTG 
ATGGTGTTTG ACCTGGCAGC AGCTCTCAAA TATCTACACA GAATGTGCAT CGTTCATCGA 
GACATCAAAC CTGAAAATTT ACTGGTGTGT GAGTACCCTA ATGGTACAAA GTCTTTGAAG 
TTGGGGGATT TTGGCTTGGC GACAGTGGTT GAAGGGCCGC TGTACACTGT TTGTGGAACA 
CCGACTTATG TTGCCCCGGA AATCATAGCT GAGTCAGGCT ACGGGCTGAA AGTGGACATC 
TGGGCAGCTG GAGTGATTAC TTACATCCTA CTGTGTGGCT TCCCACCTTT CCGCAGTGAG 
AATAATCTAC AGGAGGACCT ATTTGATCAG ATCCTGCTGG GGCATTTAGA GTTCCCCTCA 
CCATTCTGGG ACAATATCTC TGATTCTGCT AAAGAGTTGA TTGGTCACAT GCTGCAGGTG 
AATGTGGAGG CCAGATACAC AGCGGAAGAT GTGCTCTCTC ACCCCTGGGT CACAGAGGAT 
GCAGCAATGG AAAACAACAT GAAGATGGAG GTGGCAGGTA AACTCAAAAA GCACTTTAAC 
TCTGTGCAGA AACAGAGCAA CACTTCTGCT GGAGTTTCGG TTATCATGCT CCAAGGCAAT 
GAACACAGCT CTAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dclk2a ( NM_001145789.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001139261.1 Danio rerio doublecortin-like kinase 2a (dclk2a), transcript variant 4, mRNA.

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CloneID	ODa36626
Clone ID Related Accession (Same CDS sequence)	NM_001145789.1
Accession Version	NM_001145789.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2169bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-22
Organism	Danio rerio(zebrafish)
Product	serine/threonine-protein kinase DCLK2 isoform 4
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AB266178.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB266178.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA4476763, SAMEA4476764 [ECO:0000348] ##Evidence-Data-END##

ATGTCCAGCA GAAACATCGA GTGGGAGCAC TTCGAGGAGC GCGAGAAGGG TCACCGGTCC 
CCGCGGGGCA GCGGCGGCTC CCATTCCGGC TCTCGGGGAA ACGGGATCGT GCCGAGTCCC 
GCTCACAGCG CGCACTGTAG CTTTTACCGC ACGCGCACAC TGCAGTCGCT CACCTCGGAG 
AAGAAAGCAA AAAAAGTTCG CTTTTATCGG AATGGCGACA AGTATTTCAA GGGGCTTGTG 
TACGCCGTGT CCGGTGACCG CTTCAGGTCT TTCGACGCGC TGCTGATGGA GCTCACGCGC 
TCCCTCTCGG ACAATGTCAA TTTACCGCAG GGAGTCCGGA GCATCTACAC TGCAGATGGA 
GGAAAGAAGA TCACCAGTCT GGATGACTTG GTAGAAGGAG AAAGCTACGT ATGTGCTTCC 
AACGAACCTT TTCGTAAAGT GGACTACACC AAGAATGTTA ATCCCAACTG GTCAGTCAAT 
GTGAAAACTG GTGCGTCCCG CTCCATGCCA TCACTTACCG CCACTAAAAA TGAGCTGAGA 
GAACGAGAGA GCAAAGACTA CATTAAGCCT AAGCTGGTGA CAGTCATCAG GAGTGGTGTT 
AAACCGCGTA AGGCTGTTCG AATTCTCCTG AACAAGAAAA CGGCTCACTC TTTTGAGCAG 
GTGCTCACCG ACATCACTGA CGCCATTAAA CTGGACTCCG GGGCCGTGAA GAGACTCTAC 
ACTCTGGAAG GCAAACAAAT TATATGTTTG CAGGATTTCT TTGGAGATGA TGACGTTTTC 
ATCGCGTGCG GCCCTGAGAA GTACCGTTAT GCACAGGATG ATTTTGTGTT GGATCACAGT 
GGTAAGGCTT CCACAGCCTT TATAACCGAA TGTCAGGTGA TCAAGTCCTC TTATTCTGCT 
CGCTCTGCTC CCCCTGTGAG ATACTCTGGA AGTAAAAGTC CAGGAACTGG CCGTCGCAGT 
AAATCCCCTG GATCTGTAAA CGGTGCACCC AACAGCCAAA TCACTACCCC CAAATCCGCC 
AAATCCTCCA GCTCGTCCCC GACCAGCCCC CGCAGTATGC GCAACTTCAA GTTTAGTTGC 
AACAAGCGGA TGGGAGTCCC TGTCCGTCTC TCTGTACAGA TTCCGACACA TCACTCCTCC 
GCAGGCAATG TGAACGGCTC CTCTGAGACG CCTCAGCATC ATAACAACAG CTTGAGCCCT 
GAAGTTAACG GAAACAGGAA CCTTGCTGCT TCCACTATTT TGGACAAGTA CAAAATTGGC 
AAAGTTATCG GTGATGGAAA CTTTGCTGTG GTGAAGGAAT GTGTTGAACG ATCCACTGGA 
AAGGAGTTTG CACTGAAGAT CATCGATAAA AACAAATGCA GAGGCAAAGA GCACCTGATT 
GAGAATGAGG TGGCAGTGCT GAGGCGAGTT AAACATCCTA ACATCATCAT GCTGATTGAG 
GAGGTGGATA CGCCTGCTGA GCTCTATCTG GTCATGGAAC TTGTGAAGGG TGGAGATCTG 
TTTGATGCAA TCACCTCCTC AACCAAATAC ACTGAAAAAG ATGCCAGCGT GATGGTGTTT 
GACCTGGCAG CAGCTCTCAA ATATCTACAC AGAATGTGCA TCGTTCATCG AGACATCAAA 
CCTGAAAATC TACTGGTGTG TGAGTACCCT AATGGTACAA AGTCTTTGAA GTTGGGGGAT 
TTTGGCTTGG CGACAGTGGT TGAAGGGCCG CTGTACACTG TTTGTGGAAC ACCGACTTAT 
GTTGCCCCGG AAATCATAGC TGAGTCAGGC TACGGGCTGA AAGTGGACAT CTGGGCAGCT 
GGAGTGATTA CCTACATCCT ACTGTGTGGC TTCCCACCTT TCCGCAGTGA GAATAATCTA 
CAGGAGGACC TATTTGATCA GATCCTGCTG GGGCATTTAG AGTTCCCTTC ACCATTCTGG 
GACAATATCT CTGATTCTGC TAAAGAGTTG ATTGGTCACA TGCTGCAGGT GAATGTGGAG 
GCCAGATACA CAGCGGAAGA TGTGCTCTCT CACCCCTGGG TCACAGAGGA TGCAGCAATG 
GAAAACAACA TGAAGATGGA GGTGGCAGGT AAACTTAAAA AGCACTTTAA CTCTGTGCAG 
AAACAGAGCA ACACTTCTGC TGGAGTTTCG GTTATCATGC TCCAAGGCAA TGAACACAGC 
TCTAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001139261.1
CDS	156..2324
Translation	MSSRNIEWEHFEEREKGHRSPRGSGGSHSGSRGNGIVPSPAHSAHCSFYRTRTLQSLTSEKKAKKVRFYRNGDKYFKGLVYAVSGDRFRSFDALLMELTRSLSDNVNLPQGVRSIYTADGGKKITSLDDLVEGESYVCASNEPFRKVDYTKNVNPNWSVNVKTGASRSMPSLTATKNELRERESKDYIKPKLVTVIRSGVKPRKAVRILLNKKTAHSFEQVLTDITDAIKLDSGAVKRLYTLEGKQIICLQDFFGDDDVFIACGPEKYRYAQDDFVLDHSGKASTAFITECQVIKSSYSARSAPPVRYSGSKSPGTGRRSKSPGSVNGAPNSQITTPKSAKSSSSSPTSPRSMRNFKFSCNKRMGVPVRLSVQIPTHHSSAGNVNGSSETPQHHNNSLSPEVNGNRNLAASTILDKYKIGKVIGDGNFAVVKECVERSTGKEFALKIIDKNKCRGKEHLIENEVAVLRRVKHPNIIMLIEEVDTPAELYLVMELVKGGDLFDAITSSTKYTEKDASVMVFDLAAALKYLHRMCIVHRDIKPENLLVCEYPNGTKSLKLGDFGLATVVEGPLYTVCGTPTYVAPEIIAESGYGLKVDIWAAGVITYILLCGFPPFRSENNLQEDLFDQILLGHLEFPSPFWDNISDSAKELIGHMLQVNVEARYTAEDVLSHPWVTEDAAMENNMKMEVAGKLKKHFNSVQKQSNTSAGVSVIMLQGNEHSSR

Target ORF information:

RefSeq Version	NM_001145789.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio doublecortin-like kinase 2a (dclk2a), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001145789.1

ORF Insert Sequence:

ATGTCCAGCA GAAACATCGA GTGGGAGCAC TTCGAGGAGC GCGAGAAGGG TCACCGGTCC 
CCGCGGGGCA GCGGCGGCTC CCATTCCGGC TCTCGGGGAA ACGGGATCGT GCCGAGTCCC 
GCTCACAGCG CGCACTGTAG CTTTTACCGC ACGCGCACAC TGCAGTCGCT CACCTCGGAG 
AAGAAAGCAA AAAAAGTTCG CTTTTATCGG AATGGCGACA AGTATTTCAA GGGGCTTGTG 
TACGCCGTGT CCGGTGACCG CTTCAGGTCT TTCGACGCGC TGCTGATGGA GCTCACGCGC 
TCCCTCTCGG ACAATGTCAA TTTACCGCAG GGAGTCCGGA GCATCTACAC TGCAGATGGA 
GGAAAGAAGA TCACCAGTCT GGATGACTTG GTAGAAGGAG AAAGCTACGT ATGTGCTTCC 
AACGAACCTT TTCGTAAAGT GGACTACACC AAGAATGTTA ATCCCAACTG GTCAGTCAAT 
GTGAAAACTG GTGCGTCCCG CTCCATGCCA TCACTTACCG CCACTAAAAA TGAGCTGAGA 
GAACGAGAGA GCAAAGACTA CATTAAGCCT AAGCTGGTGA CAGTCATCAG GAGTGGTGTT 
AAACCGCGTA AGGCTGTTCG AATTCTCCTG AACAAGAAAA CGGCTCACTC TTTTGAGCAG 
GTGCTCACCG ACATCACTGA CGCCATTAAA CTGGACTCCG GGGCCGTGAA GAGACTCTAC 
ACTCTGGAAG GCAAACAAAT TATATGTTTG CAGGATTTCT TTGGAGATGA TGACGTTTTC 
ATCGCGTGCG GCCCTGAGAA GTACCGTTAT GCACAGGATG ATTTTGTGTT GGATCACAGT 
GGTAAGGCTT CCACAGCCTT TATAACCGAA TGTCAGGTGA TCAAGTCCTC TTATTCTGCT 
CGCTCTGCTC CCCCTGTGAG ATACTCTGGA AGTAAAAGTC CAGGAACTGG CCGTCGCAGT 
AAATCCCCTG GATCTGTAAA CGGTGCACCC AACAGCCAAA TCACTACCCC CAAATCCGCC 
AAATCCTCCA GCTCGTCCCC GACCAGCCCC CGCAGTATGC GCAACTTCAA GTTTAGTTGC 
AACAAGCGGA TGGGAGTCCC TGTCCGTCTC TCTGTACAGA TTCCGACACA TCACTCCTCC 
GCAGGCAATG TGAACGGCTC CTCTGAGACG CCTCAGCATC ATAACAACAG CTTGAGCCCT 
GAAGTTAACG GAAACAGGAA CCTTGCTGCT TCCACTATTT TGGACAAGTA CAAAATTGGC 
AAAGTTATCG GTGATGGAAA CTTTGCTGTG GTGAAGGAAT GTGTTGAACG ATCCACTGGA 
AAGGAGTTTG CACTGAAGAT CATCGATAAA AACAAATGCA GAGGCAAAGA GCACCTGATT 
GAGAATGAGG TGGCAGTGCT GAGGCGAGTT AAACATCCTA ACATCATCAT GCTGATTGAG 
GAGGTGGATA CGCCTGCTGA GCTCTATCTG GTCATGGAAC TTGTGAAGGG TGGAGATCTG 
TTTGATGCAA TCACCTCCTC AACCAAATAC ACTGAAAAAG ATGCCAGCGT GATGGTGTTT 
GACCTGGCAG CAGCTCTCAA ATATCTACAC AGAATGTGCA TCGTTCATCG AGACATCAAA 
CCTGAAAATC TACTGGTGTG TGAGTACCCT AATGGTACAA AGTCTTTGAA GTTGGGGGAT 
TTTGGCTTGG CGACAGTGGT TGAAGGGCCG CTGTACACTG TTTGTGGAAC ACCGACTTAT 
GTTGCCCCGG AAATCATAGC TGAGTCAGGC TACGGGCTGA AAGTGGACAT CTGGGCAGCT 
GGAGTGATTA CCTACATCCT ACTGTGTGGC TTCCCACCTT TCCGCAGTGA GAATAATCTA 
CAGGAGGACC TATTTGATCA GATCCTGCTG GGGCATTTAG AGTTCCCTTC ACCATTCTGG 
GACAATATCT CTGATTCTGC TAAAGAGTTG ATTGGTCACA TGCTGCAGGT GAATGTGGAG 
GCCAGATACA CAGCGGAAGA TGTGCTCTCT CACCCCTGGG TCACAGAGGA TGCAGCAATG 
GAAAACAACA TGAAGATGGA GGTGGCAGGT AAACTTAAAA AGCACTTTAA CTCTGTGCAG 
AAACAGAGCA ACACTTCTGC TGGAGTTTCG GTTATCATGC TCCAAGGCAA TGAACACAGC 
TCTAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dclk2a ( XM_009292593.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009290868.1 Danio rerio doublecortin-like kinase 2a (dclk2a), transcript variant X1, mRNA.

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CloneID	ODa05912
Clone ID Related Accession (Same CDS sequence)	XM_009292593.3 , XM_009292593.2
Accession Version	XM_009292593.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2406bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	serine/threonine-protein kinase DCLK2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009292593.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAGCA GAAACATCGA GTGGGAGCAC TTCGAGGAGC GCGAGAAGGG TCACCGGTCC 
CCGCGGGGCA GCGGCGGCTC CCATTCCGGC TCTCGGGGAA ACGGGATCGT GCCGAGTCCC 
GCTCACAGCG CGCACTGTAG CTTTTACCGC ACGCGCACAC TGCAGTCGCT CACCTCGGAG 
AAGAAAGCGA AAAAAGTTCG CTTTTATCGG AATGGCGACA AGTATTTCAA GGGGCTTGTG 
TACGCCGTGT CCGGTGACCG CTTCAGGTCT TTCGACGCGC TGCTGATGGA GCTCACGCGC 
TCCCTCTCGG ACAATGTCAA TTTACCGCAG GGAGTCCGGA GCATCTACAC TGCAGATGGA 
GGAAAGAAGA TCACCAGTCT GGATGACTTG GTAGAAGGAG AAAGCTACGT ATGTGCTTCC 
AACGAACCTT TTCGTAAAGT GGACTACACC AAGAATGTTA ATCCCAACTG GTCAGTCAAT 
GTGAAAACTG GTGCGTCCCG CTCCATGCCA TCACTTACCG CCACTAAAAA TGAGCTGAGA 
GAACGAGAGA GCAAAGACTA TATTAAGCCC AAGCTGGTGA CAGTCATCAG GAGTGGTGTT 
AAACCGCGTA AGGCTGTTCG AATTCTCCTG AACAAGAAAA CGGCTCACTC TTTTGAGCAG 
GTGCTCACAG ACATCACTGA CGCCATTAAA CTGGACTCCG GGGCCGTGAA GAGACTCTAC 
ACTCTGGAAG GCAAACAAAT TATATGTTTG CAGGATTTCT TTGGAGATGA TGACGTTTTC 
ATCGCGTGCG GCCCTGAGAA GTACCGTTAT GCACAGGATG ATTTTGTGTT GGATCACAGT 
GAATGTCAGG TGATCAAGTC CTCTTATTCT GCTCGCTCTG CTCCCCCTGT GAGATACTCT 
GGAAGTAAAA GTCCAGGAAC TGGCCGTCGC AGTAAATCCC CTGGATCTGC GAGGAGAACT 
GCAGGACACT TCTCCACAAA TAGTCAGTCT CCAGTCAAAT CACCAATAAA CGGTGCACCC 
AACAGCCAAA TCACTACCCC CAAATCCGCC AAATCCTCCA GCTCGTCCCC GACCAGCCCC 
CGCAGTATGC GCAACTTCAA GATTCCGACA CATCACTCCT CCGCAGGCAA TGTGAACGGC 
TCCTCTGAGA CGCCTCAGCA TCATAACAAC AGCTTGAGCC CTGAAGTTAA CGGAAACAGG 
AACCTTGCTG CTTCCACTAT TTTGGACAAG TACAAAATTG GCAAAGTTAT CGGTGATGGA 
AACTTTGCTG TGGTGAAGGA ATGTGTTGAA CGATCCACTG GAAAGGAGTT TGCACTGAAG 
ATCATCGATA AAAACAAATG CAGAGGCAAA GAGCACCTGA TTGAGAATGA GGTGGCAGTG 
CTGAGGCGAG TTAAACATCC TAACATCATC ATGCTGATTG AGGAGGTGGA TACGCCTGCT 
GAGCTCTATC TGGTCATGGA ACTTGTGAAG GGTGGAGATC TGTTTGATGC AATCACCTCC 
TCAACCAAAT ACACTGAAAA AGATGCCAGC GTGATGGTGT TTGACCTGGC AGCAGCTCTC 
AAATATCTAC ACAGAATGTG CATCGTTCAT CGAGACATCA AACCTGAAAA TTTACTGGTG 
TGTGAGTACC CTAATGGTAC AAAGTCTTTG AAGTTGGGGG ATTTTGGCTT GGCGACAGTG 
GTTGAAGGGC CGCTGTACAC TGTTTGTGGA ACACCGACTT ATGTTGCCCC GGAAATCATA 
GCTGAGTCAG GCTACGGGCT GAAAGTGGAC ATCTGGGCAG CTGGAGTGAT TACTTACATC 
CTACTGTGTG GCTTCCCACC TTTCCGCAGT GAGAATAATC TACAGGAGGA CCTATTTGAT 
CAGATCCTGC TGGGGCATTT AGAGTTCCCC TCACCATTCT GGGACAATAT CTCTGATTCT 
GCTAAAGAGT TGATTGGTCA CATGCTGCAG GTGAATGTGG AGGCCAGATA CACAGCGGAA 
GATGTGCTCT CTCACCCCTG GGTCACAGAG GATGCAGCAA TGGAAAACAA CATGAAGATG 
GAGGTGGCAG GTAAACTCAA AAAGCACTTT AACTCTGTGC AGAAACAGAG CAACACTTCT 
GCTGGAGTTT CGGTTATCAT GAACACAGCT CTAGATAAGG AAACTATCCA GCTGTCCCAC 
CGCCGCCAGG ACCGCAACCC TCCTCGTCCA GAAAAGAGAC CCTCCTCCAC CAACCCAGAA 
AGACGACGTT CCAACAGGGG ACGCTCATCT GTCCAGTCGG AGGACTCTAG AAGTGCTGCT 
CCCATTGAGC CTGCAACCTC AGCAAACCCC AGTGCACCAG CAGCACTCCC TTCTGACCCT 
TCTCCGCAAT TCCATGAATG CAATACTGAG TCTGAGGCCA GTAAAGACCT CCTGAAGGAA 
GAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009290868.1
CDS	410..2815
Translation	MSSRNIEWEHFEEREKGHRSPRGSGGSHSGSRGNGIVPSPAHSAHCSFYRTRTLQSLTSEKKAKKVRFYRNGDKYFKGLVYAVSGDRFRSFDALLMELTRSLSDNVNLPQGVRSIYTADGGKKITSLDDLVEGESYVCASNEPFRKVDYTKNVNPNWSVNVKTGASRSMPSLTATKNELRERESKDYIKPKLVTVIRSGVKPRKAVRILLNKKTAHSFEQVLTDITDAIKLDSGAVKRLYTLEGKQIICLQDFFGDDDVFIACGPEKYRYAQDDFVLDHSECQVIKSSYSARSAPPVRYSGSKSPGTGRRSKSPGSARRTAGHFSTNSQSPVKSPINGAPNSQITTPKSAKSSSSSPTSPRSMRNFKIPTHHSSAGNVNGSSETPQHHNNSLSPEVNGNRNLAASTILDKYKIGKVIGDGNFAVVKECVERSTGKEFALKIIDKNKCRGKEHLIENEVAVLRRVKHPNIIMLIEEVDTPAELYLVMELVKGGDLFDAITSSTKYTEKDASVMVFDLAAALKYLHRMCIVHRDIKPENLLVCEYPNGTKSLKLGDFGLATVVEGPLYTVCGTPTYVAPEIIAESGYGLKVDIWAAGVITYILLCGFPPFRSENNLQEDLFDQILLGHLEFPSPFWDNISDSAKELIGHMLQVNVEARYTAEDVLSHPWVTEDAAMENNMKMEVAGKLKKHFNSVQKQSNTSAGVSVIMNTALDKETIQLSHRRQDRNPPRPEKRPSSTNPERRRSNRGRSSVQSEDSRSAAPIEPATSANPSAPAALPSDPSPQFHECNTESEASKDLLKEE

Target ORF information:

RefSeq Version	XM_009292593.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio doublecortin-like kinase 2a (dclk2a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009292593.3

ORF Insert Sequence:

ATGTCCAGCA GAAACATCGA GTGGGAGCAC TTCGAGGAGC GCGAGAAGGG TCACCGGTCC 
CCGCGGGGCA GCGGCGGCTC CCATTCCGGC TCTCGGGGAA ACGGGATCGT GCCGAGTCCC 
GCTCACAGCG CGCACTGTAG CTTTTACCGC ACGCGCACAC TGCAGTCGCT CACCTCGGAG 
AAGAAAGCGA AAAAAGTTCG CTTTTATCGG AATGGCGACA AGTATTTCAA GGGGCTTGTG 
TACGCCGTGT CCGGTGACCG CTTCAGGTCT TTCGACGCGC TGCTGATGGA GCTCACGCGC 
TCCCTCTCGG ACAATGTCAA TTTACCGCAG GGAGTCCGGA GCATCTACAC TGCAGATGGA 
GGAAAGAAGA TCACCAGTCT GGATGACTTG GTAGAAGGAG AAAGCTACGT ATGTGCTTCC 
AACGAACCTT TTCGTAAAGT GGACTACACC AAGAATGTTA ATCCCAACTG GTCAGTCAAT 
GTGAAAACTG GTGCGTCCCG CTCCATGCCA TCACTTACCG CCACTAAAAA TGAGCTGAGA 
GAACGAGAGA GCAAAGACTA TATTAAGCCC AAGCTGGTGA CAGTCATCAG GAGTGGTGTT 
AAACCGCGTA AGGCTGTTCG AATTCTCCTG AACAAGAAAA CGGCTCACTC TTTTGAGCAG 
GTGCTCACAG ACATCACTGA CGCCATTAAA CTGGACTCCG GGGCCGTGAA GAGACTCTAC 
ACTCTGGAAG GCAAACAAAT TATATGTTTG CAGGATTTCT TTGGAGATGA TGACGTTTTC 
ATCGCGTGCG GCCCTGAGAA GTACCGTTAT GCACAGGATG ATTTTGTGTT GGATCACAGT 
GAATGTCAGG TGATCAAGTC CTCTTATTCT GCTCGCTCTG CTCCCCCTGT GAGATACTCT 
GGAAGTAAAA GTCCAGGAAC TGGCCGTCGC AGTAAATCCC CTGGATCTGC GAGGAGAACT 
GCAGGACACT TCTCCACAAA TAGTCAGTCT CCAGTCAAAT CACCAATAAA CGGTGCACCC 
AACAGCCAAA TCACTACCCC CAAATCCGCC AAATCCTCCA GCTCGTCCCC GACCAGCCCC 
CGCAGTATGC GCAACTTCAA GATTCCGACA CATCACTCCT CCGCAGGCAA TGTGAACGGC 
TCCTCTGAGA CGCCTCAGCA TCATAACAAC AGCTTGAGCC CTGAAGTTAA CGGAAACAGG 
AACCTTGCTG CTTCCACTAT TTTGGACAAG TACAAAATTG GCAAAGTTAT CGGTGATGGA 
AACTTTGCTG TGGTGAAGGA ATGTGTTGAA CGATCCACTG GAAAGGAGTT TGCACTGAAG 
ATCATCGATA AAAACAAATG CAGAGGCAAA GAGCACCTGA TTGAGAATGA GGTGGCAGTG 
CTGAGGCGAG TTAAACATCC TAACATCATC ATGCTGATTG AGGAGGTGGA TACGCCTGCT 
GAGCTCTATC TGGTCATGGA ACTTGTGAAG GGTGGAGATC TGTTTGATGC AATCACCTCC 
TCAACCAAAT ACACTGAAAA AGATGCCAGC GTGATGGTGT TTGACCTGGC AGCAGCTCTC 
AAATATCTAC ACAGAATGTG CATCGTTCAT CGAGACATCA AACCTGAAAA TTTACTGGTG 
TGTGAGTACC CTAATGGTAC AAAGTCTTTG AAGTTGGGGG ATTTTGGCTT GGCGACAGTG 
GTTGAAGGGC CGCTGTACAC TGTTTGTGGA ACACCGACTT ATGTTGCCCC GGAAATCATA 
GCTGAGTCAG GCTACGGGCT GAAAGTGGAC ATCTGGGCAG CTGGAGTGAT TACTTACATC 
CTACTGTGTG GCTTCCCACC TTTCCGCAGT GAGAATAATC TACAGGAGGA CCTATTTGAT 
CAGATCCTGC TGGGGCATTT AGAGTTCCCC TCACCATTCT GGGACAATAT CTCTGATTCT 
GCTAAAGAGT TGATTGGTCA CATGCTGCAG GTGAATGTGG AGGCCAGATA CACAGCGGAA 
GATGTGCTCT CTCACCCCTG GGTCACAGAG GATGCAGCAA TGGAAAACAA CATGAAGATG 
GAGGTGGCAG GTAAACTCAA AAAGCACTTT AACTCTGTGC AGAAACAGAG CAACACTTCT 
GCTGGAGTTT CGGTTATCAT GAACACAGCT CTAGATAAGG AAACTATCCA GCTGTCCCAC 
CGCCGCCAGG ACCGCAACCC TCCTCGTCCA GAAAAGAGAC CCTCCTCCAC CAACCCAGAA 
AGACGACGTT CCAACAGGGG ACGCTCATCT GTCCAGTCGG AGGACTCTAG AAGTGCTGCT 
CCCATTGAGC CTGCAACCTC AGCAAACCCC AGTGCACCAG CAGCACTCCC TTCTGACCCT 
TCTCCGCAAT TCCATGAATG CAATACTGAG TCTGAGGCCA GTAAAGACCT CCTGAAGGAA 
GAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dclk2a ( XM_009292593.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009290868.1 Danio rerio doublecortin-like kinase 2a (dclk2a), transcript variant X1, mRNA.

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CloneID	ODa05912
Clone ID Related Accession (Same CDS sequence)	XM_009292593.3 , XM_009292593.2
Accession Version	XM_009292593.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2406bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	serine/threonine-protein kinase DCLK2 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009292593.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAGCA GAAACATCGA GTGGGAGCAC TTCGAGGAGC GCGAGAAGGG TCACCGGTCC 
CCGCGGGGCA GCGGCGGCTC CCATTCCGGC TCTCGGGGAA ACGGGATCGT GCCGAGTCCC 
GCTCACAGCG CGCACTGTAG CTTTTACCGC ACGCGCACAC TGCAGTCGCT CACCTCGGAG 
AAGAAAGCGA AAAAAGTTCG CTTTTATCGG AATGGCGACA AGTATTTCAA GGGGCTTGTG 
TACGCCGTGT CCGGTGACCG CTTCAGGTCT TTCGACGCGC TGCTGATGGA GCTCACGCGC 
TCCCTCTCGG ACAATGTCAA TTTACCGCAG GGAGTCCGGA GCATCTACAC TGCAGATGGA 
GGAAAGAAGA TCACCAGTCT GGATGACTTG GTAGAAGGAG AAAGCTACGT ATGTGCTTCC 
AACGAACCTT TTCGTAAAGT GGACTACACC AAGAATGTTA ATCCCAACTG GTCAGTCAAT 
GTGAAAACTG GTGCGTCCCG CTCCATGCCA TCACTTACCG CCACTAAAAA TGAGCTGAGA 
GAACGAGAGA GCAAAGACTA TATTAAGCCC AAGCTGGTGA CAGTCATCAG GAGTGGTGTT 
AAACCGCGTA AGGCTGTTCG AATTCTCCTG AACAAGAAAA CGGCTCACTC TTTTGAGCAG 
GTGCTCACAG ACATCACTGA CGCCATTAAA CTGGACTCCG GGGCCGTGAA GAGACTCTAC 
ACTCTGGAAG GCAAACAAAT TATATGTTTG CAGGATTTCT TTGGAGATGA TGACGTTTTC 
ATCGCGTGCG GCCCTGAGAA GTACCGTTAT GCACAGGATG ATTTTGTGTT GGATCACAGT 
GAATGTCAGG TGATCAAGTC CTCTTATTCT GCTCGCTCTG CTCCCCCTGT GAGATACTCT 
GGAAGTAAAA GTCCAGGAAC TGGCCGTCGC AGTAAATCCC CTGGATCTGC GAGGAGAACT 
GCAGGACACT TCTCCACAAA TAGTCAGTCT CCAGTCAAAT CACCAATAAA CGGTGCACCC 
AACAGCCAAA TCACTACCCC CAAATCCGCC AAATCCTCCA GCTCGTCCCC GACCAGCCCC 
CGCAGTATGC GCAACTTCAA GATTCCGACA CATCACTCCT CCGCAGGCAA TGTGAACGGC 
TCCTCTGAGA CGCCTCAGCA TCATAACAAC AGCTTGAGCC CTGAAGTTAA CGGAAACAGG 
AACCTTGCTG CTTCCACTAT TTTGGACAAG TACAAAATTG GCAAAGTTAT CGGTGATGGA 
AACTTTGCTG TGGTGAAGGA ATGTGTTGAA CGATCCACTG GAAAGGAGTT TGCACTGAAG 
ATCATCGATA AAAACAAATG CAGAGGCAAA GAGCACCTGA TTGAGAATGA GGTGGCAGTG 
CTGAGGCGAG TTAAACATCC TAACATCATC ATGCTGATTG AGGAGGTGGA TACGCCTGCT 
GAGCTCTATC TGGTCATGGA ACTTGTGAAG GGTGGAGATC TGTTTGATGC AATCACCTCC 
TCAACCAAAT ACACTGAAAA AGATGCCAGC GTGATGGTGT TTGACCTGGC AGCAGCTCTC 
AAATATCTAC ACAGAATGTG CATCGTTCAT CGAGACATCA AACCTGAAAA TTTACTGGTG 
TGTGAGTACC CTAATGGTAC AAAGTCTTTG AAGTTGGGGG ATTTTGGCTT GGCGACAGTG 
GTTGAAGGGC CGCTGTACAC TGTTTGTGGA ACACCGACTT ATGTTGCCCC GGAAATCATA 
GCTGAGTCAG GCTACGGGCT GAAAGTGGAC ATCTGGGCAG CTGGAGTGAT TACTTACATC 
CTACTGTGTG GCTTCCCACC TTTCCGCAGT GAGAATAATC TACAGGAGGA CCTATTTGAT 
CAGATCCTGC TGGGGCATTT AGAGTTCCCC TCACCATTCT GGGACAATAT CTCTGATTCT 
GCTAAAGAGT TGATTGGTCA CATGCTGCAG GTGAATGTGG AGGCCAGATA CACAGCGGAA 
GATGTGCTCT CTCACCCCTG GGTCACAGAG GATGCAGCAA TGGAAAACAA CATGAAGATG 
GAGGTGGCAG GTAAACTCAA AAAGCACTTT AACTCTGTGC AGAAACAGAG CAACACTTCT 
GCTGGAGTTT CGGTTATCAT GAACACAGCT CTAGATAAGG AAACTATCCA GCTGTCCCAC 
CGCCGCCAGG ACCGCAACCC TCCTCGTCCA GAAAAGAGAC CCTCCTCCAC CAACCCAGAA 
AGACGACGTT CCAACAGGGG ACGCTCATCT GTCCAGTCGG AGGACTCTAG AAGTGCTGCT 
CCCATTGAGC CTGCAACCTC AGCAAACCCC AGTGCACCAG CAGCACTCCC TTCTGACCCT 
TCTCCGCAAT TCCATGAATG CAATACTGAG TCTGAGGCCA GTAAAGACCT CCTGAAGGAA 
GAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009290868.1
CDS	428..2833
Translation	MSSRNIEWEHFEEREKGHRSPRGSGGSHSGSRGNGIVPSPAHSAHCSFYRTRTLQSLTSEKKAKKVRFYRNGDKYFKGLVYAVSGDRFRSFDALLMELTRSLSDNVNLPQGVRSIYTADGGKKITSLDDLVEGESYVCASNEPFRKVDYTKNVNPNWSVNVKTGASRSMPSLTATKNELRERESKDYIKPKLVTVIRSGVKPRKAVRILLNKKTAHSFEQVLTDITDAIKLDSGAVKRLYTLEGKQIICLQDFFGDDDVFIACGPEKYRYAQDDFVLDHSECQVIKSSYSARSAPPVRYSGSKSPGTGRRSKSPGSARRTAGHFSTNSQSPVKSPINGAPNSQITTPKSAKSSSSSPTSPRSMRNFKIPTHHSSAGNVNGSSETPQHHNNSLSPEVNGNRNLAASTILDKYKIGKVIGDGNFAVVKECVERSTGKEFALKIIDKNKCRGKEHLIENEVAVLRRVKHPNIIMLIEEVDTPAELYLVMELVKGGDLFDAITSSTKYTEKDASVMVFDLAAALKYLHRMCIVHRDIKPENLLVCEYPNGTKSLKLGDFGLATVVEGPLYTVCGTPTYVAPEIIAESGYGLKVDIWAAGVITYILLCGFPPFRSENNLQEDLFDQILLGHLEFPSPFWDNISDSAKELIGHMLQVNVEARYTAEDVLSHPWVTEDAAMENNMKMEVAGKLKKHFNSVQKQSNTSAGVSVIMNTALDKETIQLSHRRQDRNPPRPEKRPSSTNPERRRSNRGRSSVQSEDSRSAAPIEPATSANPSAPAALPSDPSPQFHECNTESEASKDLLKEE

Target ORF information:

RefSeq Version	XM_009292593.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio doublecortin-like kinase 2a (dclk2a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009292593.2

ORF Insert Sequence:

ATGTCCAGCA GAAACATCGA GTGGGAGCAC TTCGAGGAGC GCGAGAAGGG TCACCGGTCC 
CCGCGGGGCA GCGGCGGCTC CCATTCCGGC TCTCGGGGAA ACGGGATCGT GCCGAGTCCC 
GCTCACAGCG CGCACTGTAG CTTTTACCGC ACGCGCACAC TGCAGTCGCT CACCTCGGAG 
AAGAAAGCGA AAAAAGTTCG CTTTTATCGG AATGGCGACA AGTATTTCAA GGGGCTTGTG 
TACGCCGTGT CCGGTGACCG CTTCAGGTCT TTCGACGCGC TGCTGATGGA GCTCACGCGC 
TCCCTCTCGG ACAATGTCAA TTTACCGCAG GGAGTCCGGA GCATCTACAC TGCAGATGGA 
GGAAAGAAGA TCACCAGTCT GGATGACTTG GTAGAAGGAG AAAGCTACGT ATGTGCTTCC 
AACGAACCTT TTCGTAAAGT GGACTACACC AAGAATGTTA ATCCCAACTG GTCAGTCAAT 
GTGAAAACTG GTGCGTCCCG CTCCATGCCA TCACTTACCG CCACTAAAAA TGAGCTGAGA 
GAACGAGAGA GCAAAGACTA TATTAAGCCC AAGCTGGTGA CAGTCATCAG GAGTGGTGTT 
AAACCGCGTA AGGCTGTTCG AATTCTCCTG AACAAGAAAA CGGCTCACTC TTTTGAGCAG 
GTGCTCACAG ACATCACTGA CGCCATTAAA CTGGACTCCG GGGCCGTGAA GAGACTCTAC 
ACTCTGGAAG GCAAACAAAT TATATGTTTG CAGGATTTCT TTGGAGATGA TGACGTTTTC 
ATCGCGTGCG GCCCTGAGAA GTACCGTTAT GCACAGGATG ATTTTGTGTT GGATCACAGT 
GAATGTCAGG TGATCAAGTC CTCTTATTCT GCTCGCTCTG CTCCCCCTGT GAGATACTCT 
GGAAGTAAAA GTCCAGGAAC TGGCCGTCGC AGTAAATCCC CTGGATCTGC GAGGAGAACT 
GCAGGACACT TCTCCACAAA TAGTCAGTCT CCAGTCAAAT CACCAATAAA CGGTGCACCC 
AACAGCCAAA TCACTACCCC CAAATCCGCC AAATCCTCCA GCTCGTCCCC GACCAGCCCC 
CGCAGTATGC GCAACTTCAA GATTCCGACA CATCACTCCT CCGCAGGCAA TGTGAACGGC 
TCCTCTGAGA CGCCTCAGCA TCATAACAAC AGCTTGAGCC CTGAAGTTAA CGGAAACAGG 
AACCTTGCTG CTTCCACTAT TTTGGACAAG TACAAAATTG GCAAAGTTAT CGGTGATGGA 
AACTTTGCTG TGGTGAAGGA ATGTGTTGAA CGATCCACTG GAAAGGAGTT TGCACTGAAG 
ATCATCGATA AAAACAAATG CAGAGGCAAA GAGCACCTGA TTGAGAATGA GGTGGCAGTG 
CTGAGGCGAG TTAAACATCC TAACATCATC ATGCTGATTG AGGAGGTGGA TACGCCTGCT 
GAGCTCTATC TGGTCATGGA ACTTGTGAAG GGTGGAGATC TGTTTGATGC AATCACCTCC 
TCAACCAAAT ACACTGAAAA AGATGCCAGC GTGATGGTGT TTGACCTGGC AGCAGCTCTC 
AAATATCTAC ACAGAATGTG CATCGTTCAT CGAGACATCA AACCTGAAAA TTTACTGGTG 
TGTGAGTACC CTAATGGTAC AAAGTCTTTG AAGTTGGGGG ATTTTGGCTT GGCGACAGTG 
GTTGAAGGGC CGCTGTACAC TGTTTGTGGA ACACCGACTT ATGTTGCCCC GGAAATCATA 
GCTGAGTCAG GCTACGGGCT GAAAGTGGAC ATCTGGGCAG CTGGAGTGAT TACTTACATC 
CTACTGTGTG GCTTCCCACC TTTCCGCAGT GAGAATAATC TACAGGAGGA CCTATTTGAT 
CAGATCCTGC TGGGGCATTT AGAGTTCCCC TCACCATTCT GGGACAATAT CTCTGATTCT 
GCTAAAGAGT TGATTGGTCA CATGCTGCAG GTGAATGTGG AGGCCAGATA CACAGCGGAA 
GATGTGCTCT CTCACCCCTG GGTCACAGAG GATGCAGCAA TGGAAAACAA CATGAAGATG 
GAGGTGGCAG GTAAACTCAA AAAGCACTTT AACTCTGTGC AGAAACAGAG CAACACTTCT 
GCTGGAGTTT CGGTTATCAT GAACACAGCT CTAGATAAGG AAACTATCCA GCTGTCCCAC 
CGCCGCCAGG ACCGCAACCC TCCTCGTCCA GAAAAGAGAC CCTCCTCCAC CAACCCAGAA 
AGACGACGTT CCAACAGGGG ACGCTCATCT GTCCAGTCGG AGGACTCTAG AAGTGCTGCT 
CCCATTGAGC CTGCAACCTC AGCAAACCCC AGTGCACCAG CAGCACTCCC TTCTGACCCT 
TCTCCGCAAT TCCATGAATG CAATACTGAG TCTGAGGCCA GTAAAGACCT CCTGAAGGAA 
GAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Adaptive optics improves multiphoton super-resolution imaging.
Nature methods14(9)869-872(2017 Sep)
Zheng W,Wu Y,Winter P,Fischer R,Nogare DD,Hong A,McCormick C,Christensen R,Dempsey WP,Arnold DB,Zimmerberg J,Chitnis A,Sellers J,Waterman C,Shroff H

Microtubule-actin crosslinking factor 1 (Macf1) domain function in Balbiani body dissociation and nuclear positioning.
PLoS genetics13(9)e1006983(2017 Sep)
Escobar-Aguirre M,Zhang H,Jamieson-Lucy A,Mullins MC

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dclk2a cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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