dock1 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	dock1
Entrez Gene ID	572187
Full Name	dedicator of cytokinesis 1
General protein information	Preferred Names dedicator of cytokinesis 1 Names dedicator of cytokinesis protein 1
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001110011.1	NP_001103481.1	dedicator of cytokinesis protein 1

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Dock1	NP_001028592.1
Danio rerio (zebrafish)	dock1	NP_001103481.1
Bos taurus (cattle)	DOCK1	XP_005225929.1
Rattus norvegicus (Norway rat)	Dock1	NP_001137330.1
Caenorhabditis elegans (roundworm)	ced-5	NP_501938.1
Homo sapiens (human)	DOCK1	NP_001371.1
Canis lupus familiaris (dog)	DOCK1	XP_544064.4
Drosophila melanogaster (fruit fly)	mbc	NP_001247267.1
Xenopus tropicalis (tropical clawed frog)	dock1	XP_002932708.2
Gallus gallus (chicken)	DOCK1	XP_421819.4
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004320	XP_313591.5

Gene Ontology
Bibliography

Related articles in PubMed

ADAM12-deficient zebrafish exhibit retardation in body growth at the juvenile stage without developmental defects.
Tokumasu Y, Iida A, Wang Z, Ansai S, Kinoshita M, Sehara-Fujisawa A
Development, growth & differentiation58(4)409-21(2016 May)

The bipartite rac1 Guanine nucleotide exchange factor engulfment and cell motility 1/dedicator of cytokinesis 180 (elmo1/dock180) protects endothelial cells from apoptosis in blood vessel development.
Sch?ker K, Bartsch S, Patry C, Stoll SJ, Hillebrands JL, Wieland T, Kroll J
The Journal of biological chemistry290(10)6408-18(2015 Mar)

Apoptotic cells are cleared by directional migration and elmo1- dependent macrophage engulfment.
van Ham TJ, Kokel D, Peterson RT
Current biology : CB22(9)830-6(2012 May)

The Rac1 regulator ELMO1 controls vascular morphogenesis in zebrafish.
Epting D, Wendik B, Bennewitz K, Dietz CT, Driever W, Kroll J
Circulation research107(1)45-55(2010 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

A novel signaling cascade is identified in which ELMO1/DOCK180 regulates vasculature formation in zebrafish.
Title: The Rac1 regulator ELMO1 controls vascular morphogenesis in zebrafish.

The fusion of embryonic fast-twitch myoblasts requires the activities of Dock1 and the closely related Dock5 protein.
Title: A role for the Myoblast city homologues Dock1 and Dock5 and the adaptor proteins Crk and Crk-like in zebrafish myoblast fusion.

The following dock1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the dock1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa32445	NM_001110011.1 Latest version!	Danio rerio dedicator of cytokinesis 1 (dock1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

dock1 ( NM_001110011.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001103481.1 Danio rerio dedicator of cytokinesis 1 (dock1), mRNA.

Price & Availability↑

CloneID	ODa32445
Clone ID Related Accession (Same CDS sequence)	NM_001110011.1
Accession Version	NM_001110011.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5601bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-17
Organism	Danio rerio(zebrafish)
Product	dedicator of cytokinesis protein 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AM888370.1. On Oct 21, 2007 this sequence version replaced XM_695872.2. ##Evidence-Data-START## Transcript exon combination :: AM888370.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGTCGCGCT GGGTGCCTAC AAAAAAGGAG AAGTACGGAG TGGCCGTCTA CAACTATGAC 
GCTCGCAGTA ACGAAGAGCT GTCCTTACAG ATCGGAGACA CTGTTCACAT ACTGGAGACT 
TACGAAGGAT GGTATCGAGG GTATCGGCTG AGGAGAAAAT CCAAGAAGGG TGTGTTTCCG 
GCTTGTTATA TTCATCTGAA GGAGGCTACG GTTGAAGGCA GCGGGCAAAA GGAGACTGTG 
ATTCCTACCG AACTTCCTCT GGTTCATGAG GTCACGACAA CGTTACGAGA ATGGGCCGCA 
ATATGGAGAG ACTTATATGT GGGCGACAAG CGGGAGATGT TCAACACGGT CCGAGACATG 
ATCTACGACC TGATCGAGTG GCGCTCGCAG ATCCTGTCCG GCACACTCCC ACAGGACGAG 
CTGACTGAAC TCAAACAGAG AGTCACCTCC AAGATGGACT ACGGGAACAA GTATTTAGAC 
CTGGATCTGG TGGTGAGAGA TAAAGATGGG AATATTCTGG ATCCCGACCT CACCAGCACC 
GTCAATCTGT TCCGAGCGCA CGAAACCGCG TCCAAGCAGA TTGAAGCTCG CATCCAAGAG 
GAGAAGTCTC AGAAGCAGAA CTTGGATTTG AACAGGCTGG CGAAGTTTGC CTCCACACCC 
TCGTTCGCTT TGTTCGTCAC GCTCAAGAAT GTCGTCTGCA AAATCGGAGA AGATGCGGAA 
GTGCTGATGT CACTCTATGA CCCCATTGAG TCCAAGTTTA TAAGTGAGAA TTATCTTGTG 
CGCTGGTCCA GCTCTGGTTT GGTGAAAGAC ATCGATCAGC TTCATAACCT ACGGGCCGTA 
TTTACTGATC TGGGGAGTGA AGATCTGAAG AGGGAGAAAA TCAGTTTTGT TTGTCAGATT 
GTTCGAGTGG GCCGAATGGA GCTCAGAGAT AACAACACAA AAAAATTAAC GTCAGGTCTC 
CGCAGACCCT TCGGTGTGGC AGTAATGGAC GTGACAGACA TCATCACAGG AAAGATGGAC 
GATGAGGACA AACAACATTT CATTCCTTTC CAGCCGGTGG CAGGGGAGAG CGACTTCCTG 
CAGAGCGTCA TCAACAAGGT CATCGCTGCC AAAGAGGTCA ATCACAAAGG TCAAGGTTTG 
TGGGTCACGC TGAAGCTTCT TCCAGGTGAC ATTCATCAGA TCCGCAAGGA TTTTCCTCAT 
CTGGTGGATC GATCCACTGC AGTCGCTCGC AAAATGGGCT TCCCGGAAAT CATCATGCCA 
GGTGATGTTC GTAATGACAT CTACGTGACG CTGGTTCAGG GTGATTTTGA CAAAGGCAGT 
AAAACGACAC CCAAGAACGT GGAGGTTACC ATGTCTGTAC ACGACGAAGA TGGCAAGAAA 
CTTGAGAGCG TGATCTTCCC AGGTGCTGGA GATGAAGGCA TTCTGGAGTA CAAATCTGTC 
ATTTATTATC AGGTCAAACA GCCGCGCTGG TATGAAACCA TCAAGGTTGC GATCCCGATT 
GAAGATGTAA ACAGGAGTCA CCTGCGTTTC ACCTTCAAGC ATCGCTCATC TCAAGACTGC 
AAAGATAAAT CTGAGAAGAA CTTTGCTCTG TCGTTTGTCA AGCTGATGAG ATATGATGGC 
ACAACGCTGA GGGACGGAGA ACATGACCTC ATCGTCTATA AGGCCGAAGC CAAGAAGCTG 
GAAGACTCCT CTATGTACCT GAGCTTGGCC TCCACCAAAC CAGAGATGGA GGAGAGAAAT 
CCGTCATCTG GGAAAAACGC ACAGAATCTG GGAAGTTTCA CCATCAGCAA AGACTCCTTT 
CAGATCTCCA CTCTGGTTTG CTCCACTAAA CTCACACAGA ACGTGGATCT GCTGGGTTTG 
CTGAAATGGA GATCCAACAC CAGTTTACTG CAGCAGAACC TGCGGCACCT GATGAAGGTG 
GAGGGTGGAG AAGTGGTCAA GTTTCTGCAG GACACACTGG ATGCTCTCTT CAACATTATG 
ATGGAGAACT CAGACAGTGA AACTTTTGAC ACTCTTGTGT TTGATGCTCT GGTCTTCATC 
ATCGGGCTGA TCGCAGACAG AAAGTTCCAT CATTTCAATC CAGTGTTGGA GACGTACATC 
CGCAAACACT TCAGCGCAAC CCTCGCCTAC ACAAAGCTGA CTAAAGTGCT GAAGAACTAT 
GTGGATAACG CAGAGAAGCT GTCGGAGCAG CTGCTGAAGG CTCTGAAGGC CATCGAGTAC 
ATCTTCAAGT TCATCGTCAG ATCCAGAGTT CTGTTCAACC AGCTTTATGA GAACAAAGGA 
GAGAGTGATT TCATGGAGTC CCTCAGGAAT CTCTTCGCTT CGTTTAATGC CATGATGAAC 
AGCGGTGCTG AAGGGACCGG AGGGGTGAAG GGTGCTGCAC TCAAATACGT CCCGACAATC 
GTCAATGATC TCAAACTGGT CTTTGACCCA AAGGAGCTCA GTAAACTCTT CAGTGAGTTT 
ATCCTTAAAG TTCCCCTGGG GAGACTGGTG AAGCAGAAGC TCCACTGCAT GATCGACATC 
GTCCACAGCG ACCTGTTTAC TCAGCATGAC TGCAGAGAGA TCCTGTTGCC TCTGATGACA 
GATCAGCTCA AACTTCATTT GGAGAATCAT GAAGAGCTGG AGTCCTGCTG TCAGCTGCTC 
AGCGACATAC TGGAGGTGCT TTACAGGAAA GACGTGGGCC CGACGCAGTG GCACGTGCAG 
ATCATCATGG AGAAACTGCT GAGGACCATC AACAGGACAG TGATCTCCAT GGGCAGAGAC 
TCTTCTCTCA TAGGCAGTTT TGTGGCCTGT ATGACCGGCA CACTACGGCA GATGGACGAC 
TATCACTACA CACACCTCAT CAGCACTTTT GGGAAGATGC GCACAGACGT GGTGGATTTC 
CTCATGGAGA CGTTTATCAT GTTTAAGGAC CTGATAGGGA AGAACGTCTA TCCTGGAGAC 
TGGGTTATAA TGAACATGAT GCAGAATAAA GTCTTCCTGC GAGCCATCAA TCAATACGCC 
GCCGTTCTCA GCAAGAAGTT CCTGGATCAA ACCAACTTTG AGCTGCAGTT GTGGAATAAC 
TACTTTCATC TTGCCGTGGC GTTCCTTACC CAGGAGTCTT TGCAGCTGGA GAACTTTTCG 
AGTGACAAAC GGGCCAAGAT CTTCCACAAA TATCAAGACA TGAGGCGACA GATTGGATTC 
GAGATCCGAG ACATGTGGTA CAACCTGGGT CCACACAAGA TCAAGTTCAT CCCGGAGATG 
GTGGGTCCCA TTCTGGAGAT GACGCTGGTT CCGGAGATCG AGCTGCGAAA AGCCACTATT 
CCCATCTTTT TTGACATGAT GCAGTGCGAG TTTCACTTCA GACGCTGCTT CCAGACGTTT 
GAGAACGAGA TCATCACCAA GCTGGATCAC GAGGTGGAGG GAGGCCGCGG AGATGAACAG 
TACAAAATCC TCTTCCAGAA GATTTTGTTG GAGCACTGCA GAAAGCACAA GTATCTGGCT 
AAGACTGGTG AAACCTTCGT GACTCTGGTG GTGCGTCTGT TGGAGAGACT CCTGGACTAC 
AGGACCATCA TGCATGATGA AAACAAGGAG AACCGCATGA GCTGCACAGT TAACGTGCTG 
AATTTCTACA AGGAGATTGA AAGAGAAGAG ATGTACATCA GGTACCTGTA TAAGCTGTGT 
GACCTGCATA AAGAGTGTGA CAACTATACA GAAGCCGGAT ACACATTACT GCTGCACGCC 
AAACTCCTCA AGTGGTCAGA AGAGGCCTGT GCTGCTCACC TGACGCAGAG AGACGGATAT 
CAGGCCAGCA CTCAGGGTCA ACTCAAAGAC CAGCTCTACC AGGAGATCAT CAACTACTTC 
GACAAGGGCA AGATGTGGGA AGAAGCCATC GTCCTGGGGA AGGAATTAGC AGAACAATAC 
GAGAACGAGA TGTTTGACTT TGAGCAGCTC AGCACGTTAC TGAGGAAACA GGCTCAGTTT 
TATGAGAGCA TCGTGAAGGT GATCAGACCC AAGCCGGATT ACTTTGCCGT GGGTTATAAT 
GGACTGGGCT TCCCTTCGTT CCTGCGGAAT AAGATGTTCA TCTACCGGGG TAAGGAGTAC 
GAGCGCTGGG AGGATTTCGA AGCTCGGCTG CTCACTCAGT TCCCCAACGC TGAGAAGATG 
AAGACCACCA CGCCTCCCAG CGAGGACATC CGGAACTCAT CTGGACAGTA CATTCAGTGT 
TTCACAGTGA AGCCCTTGCT AGAACTACCA CCAAAATTTC AAAACAAACC CGTATCAGAG 
CAGATCGTCA GCTTTTACAC CGTGAACGAG GTGCAGAAGT TCCAGTATTC ACGGCCGGTC 
CGGAAGGGAG AGAAAGATCC AGACAACGAG TTTTCAAACA TGTGGATTGA AAGGACGACT 
TATGTAACGG CCTATAAGCT GCCTGGTATC CTGCGCTGGT TTGAAGTCCT GTCTGTGTCA 
GCGGAGGAAA TCAGTCCTCT GGAGAATGCG ATGGAGACCA TGCAACTGAC CAATGAGAAA 
ATCAGCAACA TGGTTCAGCG TCACCTCAAT GACCCAAATC TTCCCATCAA CCCCCTCTCT 
ATGCTGCTCA ATGGCATCGT TGACCCAGCT GTCATGGGTG GCTTTACTAA TTACGAGAAG 
GCGTTCTTCA CCGAGAAATA CTTCCTAGAA CATCCAAATG ACCAGGAGAA GATTGAAAGA 
CTGAAGGATC TGATTGCTTG GCAGATTCCC AATCTGGCTG AAGGGGTGCG GATCCACGGA 
GAGAAGGTGA CAGAGGCTTT GCGGCCGTTC CACGAGCGCC TAGAAGCCTG TTTCAGACAG 
CTGAAAGAGA AGGTGGAGAA GCAGTACGGC ATCAGAACAC TACCTCCAAT GACGGATGAG 
CGGCGCGGCA GTCGACCTCG ATCAATGGTT CGTTCCTTCA CCATGCCGTC GTCTCAGAGG 
CCTCTGTCTG TGGCATCCGT CACCTCCTTA TCCTCAGACA ACTCGCCATC CAGACCGGGA 
TCAGACGGAT TTGCCTTGGA GCCTCTCCTG CCAAAGAAGC AGCACTTTAA GTCTCAGGAT 
AAACTGGACC GAGACGAGCC TGAGAAAGAC AGAAAGGAGA AGAAGAAGGA GAAGAGGAAC 
AGCAAACACC AGGAGCTGTT CGATAAAGAG CTGAAGACGG CCGACATTCC TTTGCAGGCC 
AATGAGGCTG TCATTCTGTC CGAGACGATC AGTCCATTGA GGCCCCAGAG GCCGAAGAGT 
CAAGTGATCA ACCTGCTGGG GGAGAAGAGA CTCTCGGTTT CTCCAGGAGC CCCTGCGAAC 
CAGTTTGTGC CCAGCGTTCC TCCACCCATC ACCCCACGGG CCAAACTACA GTTCAGCCTG 
CTGTCTGGAT CCAATCTGGA GCTGAATGGC ACGAGTTATG CAGATAAGGG GGAAACCCCG 
CCCCCACTGC CTGTCAAGAG CAGCATGGGT GACTATGGAA ACCTGATGGA CAACTCGGAC 
CTGGCCAGTC CGACCACACC CCCTCCACCA CCACCACACC AGCGGCACCT TCCACCCCCA 
TTACCCAGCA AGACCCCTCC GCCTCCCCCT CCAAAGACCA CTCGAAAACA GACCTCCATT 
GACTCGGGAA TCGTCCAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001103481.1
CDS	88..5688
Translation	MSRWVPTKKEKYGVAVYNYDARSNEELSLQIGDTVHILETYEGWYRGYRLRRKSKKGVFPACYIHLKEATVEGSGQKETVIPTELPLVHEVTTTLREWAAIWRDLYVGDKREMFNTVRDMIYDLIEWRSQILSGTLPQDELTELKQRVTSKMDYGNKYLDLDLVVRDKDGNILDPDLTSTVNLFRAHETASKQIEARIQEEKSQKQNLDLNRLAKFASTPSFALFVTLKNVVCKIGEDAEVLMSLYDPIESKFISENYLVRWSSSGLVKDIDQLHNLRAVFTDLGSEDLKREKISFVCQIVRVGRMELRDNNTKKLTSGLRRPFGVAVMDVTDIITGKMDDEDKQHFIPFQPVAGESDFLQSVINKVIAAKEVNHKGQGLWVTLKLLPGDIHQIRKDFPHLVDRSTAVARKMGFPEIIMPGDVRNDIYVTLVQGDFDKGSKTTPKNVEVTMSVHDEDGKKLESVIFPGAGDEGILEYKSVIYYQVKQPRWYETIKVAIPIEDVNRSHLRFTFKHRSSQDCKDKSEKNFALSFVKLMRYDGTTLRDGEHDLIVYKAEAKKLEDSSMYLSLASTKPEMEERNPSSGKNAQNLGSFTISKDSFQISTLVCSTKLTQNVDLLGLLKWRSNTSLLQQNLRHLMKVEGGEVVKFLQDTLDALFNIMMENSDSETFDTLVFDALVFIIGLIADRKFHHFNPVLETYIRKHFSATLAYTKLTKVLKNYVDNAEKLSEQLLKALKAIEYIFKFIVRSRVLFNQLYENKGESDFMESLRNLFASFNAMMNSGAEGTGGVKGAALKYVPTIVNDLKLVFDPKELSKLFSEFILKVPLGRLVKQKLHCMIDIVHSDLFTQHDCREILLPLMTDQLKLHLENHEELESCCQLLSDILEVLYRKDVGPTQWHVQIIMEKLLRTINRTVISMGRDSSLIGSFVACMTGTLRQMDDYHYTHLISTFGKMRTDVVDFLMETFIMFKDLIGKNVYPGDWVIMNMMQNKVFLRAINQYAAVLSKKFLDQTNFELQLWNNYFHLAVAFLTQESLQLENFSSDKRAKIFHKYQDMRRQIGFEIRDMWYNLGPHKIKFIPEMVGPILEMTLVPEIELRKATIPIFFDMMQCEFHFRRCFQTFENEIITKLDHEVEGGRGDEQYKILFQKILLEHCRKHKYLAKTGETFVTLVVRLLERLLDYRTIMHDENKENRMSCTVNVLNFYKEIEREEMYIRYLYKLCDLHKECDNYTEAGYTLLLHAKLLKWSEEACAAHLTQRDGYQASTQGQLKDQLYQEIINYFDKGKMWEEAIVLGKELAEQYENEMFDFEQLSTLLRKQAQFYESIVKVIRPKPDYFAVGYNGLGFPSFLRNKMFIYRGKEYERWEDFEARLLTQFPNAEKMKTTTPPSEDIRNSSGQYIQCFTVKPLLELPPKFQNKPVSEQIVSFYTVNEVQKFQYSRPVRKGEKDPDNEFSNMWIERTTYVTAYKLPGILRWFEVLSVSAEEISPLENAMETMQLTNEKISNMVQRHLNDPNLPINPLSMLLNGIVDPAVMGGFTNYEKAFFTEKYFLEHPNDQEKIERLKDLIAWQIPNLAEGVRIHGEKVTEALRPFHERLEACFRQLKEKVEKQYGIRTLPPMTDERRGSRPRSMVRSFTMPSSQRPLSVASVTSLSSDNSPSRPGSDGFALEPLLPKKQHFKSQDKLDRDEPEKDRKEKKKEKRNSKHQELFDKELKTADIPLQANEAVILSETISPLRPQRPKSQVINLLGEKRLSVSPGAPANQFVPSVPPPITPRAKLQFSLLSGSNLELNGTSYADKGETPPPLPVKSSMGDYGNLMDNSDLASPTTPPPPPPHQRHLPPPLPSKTPPPPPPKTTRKQTSIDSGIVQ

Target ORF information:

RefSeq Version	NM_001110011.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio dedicator of cytokinesis 1 (dock1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001110011.1

ORF Insert Sequence:

ATGTCGCGCT GGGTGCCTAC AAAAAAGGAG AAGTACGGAG TGGCCGTCTA CAACTATGAC 
GCTCGCAGTA ACGAAGAGCT GTCCTTACAG ATCGGAGACA CTGTTCACAT ACTGGAGACT 
TACGAAGGAT GGTATCGAGG GTATCGGCTG AGGAGAAAAT CCAAGAAGGG TGTGTTTCCG 
GCTTGTTATA TTCATCTGAA GGAGGCTACG GTTGAAGGCA GCGGGCAAAA GGAGACTGTG 
ATTCCTACCG AACTTCCTCT GGTTCATGAG GTCACGACAA CGTTACGAGA ATGGGCCGCA 
ATATGGAGAG ACTTATATGT GGGCGACAAG CGGGAGATGT TCAACACGGT CCGAGACATG 
ATCTACGACC TGATCGAGTG GCGCTCGCAG ATCCTGTCCG GCACACTCCC ACAGGACGAG 
CTGACTGAAC TCAAACAGAG AGTCACCTCC AAGATGGACT ACGGGAACAA GTATTTAGAC 
CTGGATCTGG TGGTGAGAGA TAAAGATGGG AATATTCTGG ATCCCGACCT CACCAGCACC 
GTCAATCTGT TCCGAGCGCA CGAAACCGCG TCCAAGCAGA TTGAAGCTCG CATCCAAGAG 
GAGAAGTCTC AGAAGCAGAA CTTGGATTTG AACAGGCTGG CGAAGTTTGC CTCCACACCC 
TCGTTCGCTT TGTTCGTCAC GCTCAAGAAT GTCGTCTGCA AAATCGGAGA AGATGCGGAA 
GTGCTGATGT CACTCTATGA CCCCATTGAG TCCAAGTTTA TAAGTGAGAA TTATCTTGTG 
CGCTGGTCCA GCTCTGGTTT GGTGAAAGAC ATCGATCAGC TTCATAACCT ACGGGCCGTA 
TTTACTGATC TGGGGAGTGA AGATCTGAAG AGGGAGAAAA TCAGTTTTGT TTGTCAGATT 
GTTCGAGTGG GCCGAATGGA GCTCAGAGAT AACAACACAA AAAAATTAAC GTCAGGTCTC 
CGCAGACCCT TCGGTGTGGC AGTAATGGAC GTGACAGACA TCATCACAGG AAAGATGGAC 
GATGAGGACA AACAACATTT CATTCCTTTC CAGCCGGTGG CAGGGGAGAG CGACTTCCTG 
CAGAGCGTCA TCAACAAGGT CATCGCTGCC AAAGAGGTCA ATCACAAAGG TCAAGGTTTG 
TGGGTCACGC TGAAGCTTCT TCCAGGTGAC ATTCATCAGA TCCGCAAGGA TTTTCCTCAT 
CTGGTGGATC GATCCACTGC AGTCGCTCGC AAAATGGGCT TCCCGGAAAT CATCATGCCA 
GGTGATGTTC GTAATGACAT CTACGTGACG CTGGTTCAGG GTGATTTTGA CAAAGGCAGT 
AAAACGACAC CCAAGAACGT GGAGGTTACC ATGTCTGTAC ACGACGAAGA TGGCAAGAAA 
CTTGAGAGCG TGATCTTCCC AGGTGCTGGA GATGAAGGCA TTCTGGAGTA CAAATCTGTC 
ATTTATTATC AGGTCAAACA GCCGCGCTGG TATGAAACCA TCAAGGTTGC GATCCCGATT 
GAAGATGTAA ACAGGAGTCA CCTGCGTTTC ACCTTCAAGC ATCGCTCATC TCAAGACTGC 
AAAGATAAAT CTGAGAAGAA CTTTGCTCTG TCGTTTGTCA AGCTGATGAG ATATGATGGC 
ACAACGCTGA GGGACGGAGA ACATGACCTC ATCGTCTATA AGGCCGAAGC CAAGAAGCTG 
GAAGACTCCT CTATGTACCT GAGCTTGGCC TCCACCAAAC CAGAGATGGA GGAGAGAAAT 
CCGTCATCTG GGAAAAACGC ACAGAATCTG GGAAGTTTCA CCATCAGCAA AGACTCCTTT 
CAGATCTCCA CTCTGGTTTG CTCCACTAAA CTCACACAGA ACGTGGATCT GCTGGGTTTG 
CTGAAATGGA GATCCAACAC CAGTTTACTG CAGCAGAACC TGCGGCACCT GATGAAGGTG 
GAGGGTGGAG AAGTGGTCAA GTTTCTGCAG GACACACTGG ATGCTCTCTT CAACATTATG 
ATGGAGAACT CAGACAGTGA AACTTTTGAC ACTCTTGTGT TTGATGCTCT GGTCTTCATC 
ATCGGGCTGA TCGCAGACAG AAAGTTCCAT CATTTCAATC CAGTGTTGGA GACGTACATC 
CGCAAACACT TCAGCGCAAC CCTCGCCTAC ACAAAGCTGA CTAAAGTGCT GAAGAACTAT 
GTGGATAACG CAGAGAAGCT GTCGGAGCAG CTGCTGAAGG CTCTGAAGGC CATCGAGTAC 
ATCTTCAAGT TCATCGTCAG ATCCAGAGTT CTGTTCAACC AGCTTTATGA GAACAAAGGA 
GAGAGTGATT TCATGGAGTC CCTCAGGAAT CTCTTCGCTT CGTTTAATGC CATGATGAAC 
AGCGGTGCTG AAGGGACCGG AGGGGTGAAG GGTGCTGCAC TCAAATACGT CCCGACAATC 
GTCAATGATC TCAAACTGGT CTTTGACCCA AAGGAGCTCA GTAAACTCTT CAGTGAGTTT 
ATCCTTAAAG TTCCCCTGGG GAGACTGGTG AAGCAGAAGC TCCACTGCAT GATCGACATC 
GTCCACAGCG ACCTGTTTAC TCAGCATGAC TGCAGAGAGA TCCTGTTGCC TCTGATGACA 
GATCAGCTCA AACTTCATTT GGAGAATCAT GAAGAGCTGG AGTCCTGCTG TCAGCTGCTC 
AGCGACATAC TGGAGGTGCT TTACAGGAAA GACGTGGGCC CGACGCAGTG GCACGTGCAG 
ATCATCATGG AGAAACTGCT GAGGACCATC AACAGGACAG TGATCTCCAT GGGCAGAGAC 
TCTTCTCTCA TAGGCAGTTT TGTGGCCTGT ATGACCGGCA CACTACGGCA GATGGACGAC 
TATCACTACA CACACCTCAT CAGCACTTTT GGGAAGATGC GCACAGACGT GGTGGATTTC 
CTCATGGAGA CGTTTATCAT GTTTAAGGAC CTGATAGGGA AGAACGTCTA TCCTGGAGAC 
TGGGTTATAA TGAACATGAT GCAGAATAAA GTCTTCCTGC GAGCCATCAA TCAATACGCC 
GCCGTTCTCA GCAAGAAGTT CCTGGATCAA ACCAACTTTG AGCTGCAGTT GTGGAATAAC 
TACTTTCATC TTGCCGTGGC GTTCCTTACC CAGGAGTCTT TGCAGCTGGA GAACTTTTCG 
AGTGACAAAC GGGCCAAGAT CTTCCACAAA TATCAAGACA TGAGGCGACA GATTGGATTC 
GAGATCCGAG ACATGTGGTA CAACCTGGGT CCACACAAGA TCAAGTTCAT CCCGGAGATG 
GTGGGTCCCA TTCTGGAGAT GACGCTGGTT CCGGAGATCG AGCTGCGAAA AGCCACTATT 
CCCATCTTTT TTGACATGAT GCAGTGCGAG TTTCACTTCA GACGCTGCTT CCAGACGTTT 
GAGAACGAGA TCATCACCAA GCTGGATCAC GAGGTGGAGG GAGGCCGCGG AGATGAACAG 
TACAAAATCC TCTTCCAGAA GATTTTGTTG GAGCACTGCA GAAAGCACAA GTATCTGGCT 
AAGACTGGTG AAACCTTCGT GACTCTGGTG GTGCGTCTGT TGGAGAGACT CCTGGACTAC 
AGGACCATCA TGCATGATGA AAACAAGGAG AACCGCATGA GCTGCACAGT TAACGTGCTG 
AATTTCTACA AGGAGATTGA AAGAGAAGAG ATGTACATCA GGTACCTGTA TAAGCTGTGT 
GACCTGCATA AAGAGTGTGA CAACTATACA GAAGCCGGAT ACACATTACT GCTGCACGCC 
AAACTCCTCA AGTGGTCAGA AGAGGCCTGT GCTGCTCACC TGACGCAGAG AGACGGATAT 
CAGGCCAGCA CTCAGGGTCA ACTCAAAGAC CAGCTCTACC AGGAGATCAT CAACTACTTC 
GACAAGGGCA AGATGTGGGA AGAAGCCATC GTCCTGGGGA AGGAATTAGC AGAACAATAC 
GAGAACGAGA TGTTTGACTT TGAGCAGCTC AGCACGTTAC TGAGGAAACA GGCTCAGTTT 
TATGAGAGCA TCGTGAAGGT GATCAGACCC AAGCCGGATT ACTTTGCCGT GGGTTATAAT 
GGACTGGGCT TCCCTTCGTT CCTGCGGAAT AAGATGTTCA TCTACCGGGG TAAGGAGTAC 
GAGCGCTGGG AGGATTTCGA AGCTCGGCTG CTCACTCAGT TCCCCAACGC TGAGAAGATG 
AAGACCACCA CGCCTCCCAG CGAGGACATC CGGAACTCAT CTGGACAGTA CATTCAGTGT 
TTCACAGTGA AGCCCTTGCT AGAACTACCA CCAAAATTTC AAAACAAACC CGTATCAGAG 
CAGATCGTCA GCTTTTACAC CGTGAACGAG GTGCAGAAGT TCCAGTATTC ACGGCCGGTC 
CGGAAGGGAG AGAAAGATCC AGACAACGAG TTTTCAAACA TGTGGATTGA AAGGACGACT 
TATGTAACGG CCTATAAGCT GCCTGGTATC CTGCGCTGGT TTGAAGTCCT GTCTGTGTCA 
GCGGAGGAAA TCAGTCCTCT GGAGAATGCG ATGGAGACCA TGCAACTGAC CAATGAGAAA 
ATCAGCAACA TGGTTCAGCG TCACCTCAAT GACCCAAATC TTCCCATCAA CCCCCTCTCT 
ATGCTGCTCA ATGGCATCGT TGACCCAGCT GTCATGGGTG GCTTTACTAA TTACGAGAAG 
GCGTTCTTCA CCGAGAAATA CTTCCTAGAA CATCCAAATG ACCAGGAGAA GATTGAAAGA 
CTGAAGGATC TGATTGCTTG GCAGATTCCC AATCTGGCTG AAGGGGTGCG GATCCACGGA 
GAGAAGGTGA CAGAGGCTTT GCGGCCGTTC CACGAGCGCC TAGAAGCCTG TTTCAGACAG 
CTGAAAGAGA AGGTGGAGAA GCAGTACGGC ATCAGAACAC TACCTCCAAT GACGGATGAG 
CGGCGCGGCA GTCGACCTCG ATCAATGGTT CGTTCCTTCA CCATGCCGTC GTCTCAGAGG 
CCTCTGTCTG TGGCATCCGT CACCTCCTTA TCCTCAGACA ACTCGCCATC CAGACCGGGA 
TCAGACGGAT TTGCCTTGGA GCCTCTCCTG CCAAAGAAGC AGCACTTTAA GTCTCAGGAT 
AAACTGGACC GAGACGAGCC TGAGAAAGAC AGAAAGGAGA AGAAGAAGGA GAAGAGGAAC 
AGCAAACACC AGGAGCTGTT CGATAAAGAG CTGAAGACGG CCGACATTCC TTTGCAGGCC 
AATGAGGCTG TCATTCTGTC CGAGACGATC AGTCCATTGA GGCCCCAGAG GCCGAAGAGT 
CAAGTGATCA ACCTGCTGGG GGAGAAGAGA CTCTCGGTTT CTCCAGGAGC CCCTGCGAAC 
CAGTTTGTGC CCAGCGTTCC TCCACCCATC ACCCCACGGG CCAAACTACA GTTCAGCCTG 
CTGTCTGGAT CCAATCTGGA GCTGAATGGC ACGAGTTATG CAGATAAGGG GGAAACCCCG 
CCCCCACTGC CTGTCAAGAG CAGCATGGGT GACTATGGAA ACCTGATGGA CAACTCGGAC 
CTGGCCAGTC CGACCACACC CCCTCCACCA CCACCACACC AGCGGCACCT TCCACCCCCA 
TTACCCAGCA AGACCCCTCC GCCTCCCCCT CCAAAGACCA CTCGAAAACA GACCTCCATT 
GACTCGGGAA TCGTCCAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

ADAM12-deficient zebrafish exhibit retardation in body growth at the juvenile stage without developmental defects.
Development, growth & differentiation58(4)409-21(2016 May)
Tokumasu Y,Iida A,Wang Z,Ansai S,Kinoshita M,Sehara-Fujisawa A

The bipartite rac1 Guanine nucleotide exchange factor engulfment and cell motility 1/dedicator of cytokinesis 180 (elmo1/dock180) protects endothelial cells from apoptosis in blood vessel development.
The Journal of biological chemistry290(10)6408-18(2015 Mar)
Sch?ker K,Bartsch S,Patry C,Stoll SJ,Hillebrands JL,Wieland T,Kroll J

Apoptotic cells are cleared by directional migration and elmo1- dependent macrophage engulfment.
Current biology : CB22(9)830-6(2012 May)
van Ham TJ,Kokel D,Peterson RT

The Rac1 regulator ELMO1 controls vascular morphogenesis in zebrafish.
Circulation research107(1)45-55(2010 Jul)
Epting D,Wendik B,Bennewitz K,Dietz CT,Driever W,Kroll J

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dock1 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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