trap1 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	trap1
Entrez Gene ID	571959
Full Name	TNF receptor-associated protein 1
Synonyms	fc85a11,wu:fc85a11
General protein information	Preferred Names TNF receptor-associated protein 1 Names heat shock protein 75 kDa, mitochondrial
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001113625.1	NP_001107097.1	heat shock protein 75 kDa, mitochondrial

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Trap1	NP_080784.1
Gallus gallus (chicken)	TRAP1	NP_001006175.1
Danio rerio (zebrafish)	trap1	NP_001107097.1
Drosophila melanogaster (fruit fly)	Trap1	NP_001260726.1
Caenorhabditis elegans (roundworm)	R151.7	NP_741220.2
Pan troglodytes (chimpanzee)	TRAP1	XP_001167908.1
	TRAP1	XP_001094589.2
Canis lupus familiaris (dog)	TRAP1	XP_005621672.1
Bos taurus (cattle)	TRAP1	NP_001033764.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010691	XP_311405.3
Homo sapiens (human)	TRAP1	NP_057376.2
Rattus norvegicus (Norway rat)	Trap1	NP_001034090.1
Danio rerio (zebrafish)	LOC101884569	XP_005163784.1
Xenopus tropicalis (tropical clawed frog)	trap1	XP_002932506.1

Gene Ontology
Bibliography

Related articles in PubMed

Symmetry broken and rebroken during the ATP hydrolysis cycle of the mitochondrial Hsp90 TRAP1.
Elnatan D, Betegon M, Liu Y, Ramelot T, Kennedy MA, Agard DA
eLife6(2017 Jul)

Evolution of complexity in the zebrafish synapse proteome.
Bay?s ?, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 Mar)

Comparison of PBDE congeners as inducers of oxidative stress in zebrafish.
Usenko CY, Abel EL, Kudela M, Janise A, Bruce ED
Environmental toxicology and chemistry34(5)1154-60(2015 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The crystal structure of full-length TRAP1, the mitochondrial Hsp90 molecular chaperone, in a catalytically active closed state is presented.
Title: Structural asymmetry in the closed state of mitochondrial Hsp90 (TRAP1) supports a two-step ATP hydrolysis mechanism.

The following trap1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the trap1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa01060	NM_001113625.1 Latest version!	Danio rerio TNF receptor-associated protein 1 (trap1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

trap1 ( NM_001113625.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001107097.1 Danio rerio TNF receptor-associated protein 1 (trap1), mRNA.

Price & Availability↑

CloneID	ODa01060
Clone ID Related Accession (Same CDS sequence)	NM_001113625.1
Accession Version	NM_001113625.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2160bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-03
Organism	Danio rerio(zebrafish)
Product	heat shock protein 75 kDa, mitochondrial
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC154467.1. On Jan 20, 2008 this sequence version replaced XM_695606.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC154467.1, BC171630.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168448 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

ATGATCCGAT CGCTGAATTT ACTGAAATAT TCTCTAAATG TGTCCCAGCG GGCTCAGCTC 
AGGCTGCTCT CATCCAGCAA ATGGACAGGA CACAGGATTG TGCCTGCCGC GTTCACTGGA 
GTTGACACAA ATGGCAGACA GCAGCTGCAT CAGCCCAGAC CCTTCAGACT GTGGAGCCCC 
AGCTGTTCAG GGGTCAGTGT TCATCATCGC AGCTACAGCA CACAGCAACA CACAGAGCCT 
GCAGAGGAGG AGACGCTCCA CAACATCATC ACCGACACCG AGAACGTTCA AGGATCCTTC 
TCCAAACACG AATTTCAAGC AGAGACAAAG AAACTGCTGG ATATTGTGGC CAGATCGCTG 
TATTCTGAGA AAGAGGTGTT CATCAGGGAG CTGATCTCTA ACGGCAGTGA TGCTCTGGAG 
AAACTGCGGC ACAGGATGAT CACGGCTGGA GGAGACACAG CACCAATGGA GATCCACCTG 
CAGACGGACA GTGTTAAAGG CACCTTCACC ATACAGGACA CTGGTGTGGG GATGAACAAA 
GAGGATCTGG TTTCTAACCT GGGCACGATT GCTCGATCTG GATCAAAGGC TTTTCTGGAC 
GCTCTGCAGA ATCAAGCGGA GGCCAGCAGC TCCATCATTG GTCAGTTTGG TGTGGGCTTT 
TACTCCGCCT TCATGGTGGC CGATAAGGTG GAGGTTTATT CTCAGTCGGC AGAAGCAGAT 
GCTCCTGGAT ACAAGTGGTC CTCAGACGGC TCTGGAGTGT TTGAGGTCGC TGAAGCTTCA 
GGTGTTCGAC AGGGAACCAA GATTGTGCTC CACCTTAAAG ACGACTGCAA AGAGTTTTCA 
TCAGAGGACA GAGTTAAAGA GGTGGTGACC AAGTACAGCA ACTTTGTGAG TTTCCCCATC 
TTCCTGAATG GACGGAGGCT CAACACCCTG CAGGCACTCT GGATGATGGA GCCCAAAGAC 
ATCAGCGAGT GGCAGCACGA GGAGTTTTAT CGATATGTGG CTCAAGCCTA CGATAAACCG 
CGATACACAC TCCACTACCG CGCAGACGCT CCGCTCAACA TCCGCAGTAT CTTCTACGTG 
CCAGAAATGA AGCCGTCCAT GTTCGATGTG AGTCGTGAGA TGGGCTCCAG TGTGGCTCTG 
TACAGCAGAA AAATCCTGAT CCAAACCAAA GCCACAGACA TCTTGCCCAA GTGGCTGCGC 
TTCCTACGAG GTGTTGTGGA CAGTGAGGAT ATTCCTCTAA ACCTGAGCAG AGAGCTGCTA 
CAGGAAAGCG CCCTAATCAG GAAACTGCGG GATGTTTTAC AGCAGCGTGT CATTCGATTC 
CTGTTGGACC AGAGTAAGAA GGACCCGGAG AAATACGCTC GCTTCTTCGA GGACTACGGC 
CTGTTCATGC GCGAGGGCAT CGTGACCACA GGCGAGCAGA GCGTCAAGGA GGACATTGCT 
AAACTGCTGC GCTTCGAGTC GTCAGCTCTA CCTGCGGGAC AGCAGACCAG CCTGATGGAG 
TATTCCTCCC GCATGAAGGC AGGAACCAGG AACATTTATT ACCTGTGTGC GCCGAATCGA 
CACCTGGCCG AACACTCGCC GTACTTCGAG GCCATGAAAC AGAAAGATAT GGAGGTCTTG 
TTCTGCTTTG AGCAGTTTGA CGAGCTCACG CTTCTTCACC TCCGAGAGTT CGACAGGAAG 
AAGCTGATCT CCGCTGAGAC GGACATCGTT GTAGATCACT ACAAAGAGGA GAAATTCCAG 
GACAGCAAGC CAGCATCTGA GCGGTTGAGC AGTGAACAGG CTGAAGATCT GCTGGCCTGG 
ATGAGGAATG CACTCGTTCA GAGAGTCACC AACATCAAGG TGACTCCTCG TCTGGACACT 
CACCCGGCCA TGATCACAGT GCTGGAGATG GGAGCCGCTC GACACTTTCT GCGCACACAG 
CAGCTGGCCC GCAGCTCCGA GGAGAGAGCC CAGATCCTGC AGCCCACGCT GGAGATCAAC 
ACTGGGCATG ATCTGATCAA GAAGCTTCAT GCGCTGAAGG ACTCAAACCC TGAGCTGGCA 
CAGCTTCTGC TCGAACAGAT TTACGACAAT GCCATGATCG CCGCTGGCCT GAACGAAGAC 
CCTCGTCCGA TGATCTCTCG ACTGAATCAG CTGTTGACGC GAGCGCTGGA GAAACACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001107097.1
CDS	66..2225
Translation	MIRSLNLLKYSLNVSQRAQLRLLSSSKWTGHRIVPAAFTGVDTNGRQQLHQPRPFRLWSPSCSGVSVHHRSYSTQQHTEPAEEETLHNIITDTENVQGSFSKHEFQAETKKLLDIVARSLYSEKEVFIRELISNGSDALEKLRHRMITAGGDTAPMEIHLQTDSVKGTFTIQDTGVGMNKEDLVSNLGTIARSGSKAFLDALQNQAEASSSIIGQFGVGFYSAFMVADKVEVYSQSAEADAPGYKWSSDGSGVFEVAEASGVRQGTKIVLHLKDDCKEFSSEDRVKEVVTKYSNFVSFPIFLNGRRLNTLQALWMMEPKDISEWQHEEFYRYVAQAYDKPRYTLHYRADAPLNIRSIFYVPEMKPSMFDVSREMGSSVALYSRKILIQTKATDILPKWLRFLRGVVDSEDIPLNLSRELLQESALIRKLRDVLQQRVIRFLLDQSKKDPEKYARFFEDYGLFMREGIVTTGEQSVKEDIAKLLRFESSALPAGQQTSLMEYSSRMKAGTRNIYYLCAPNRHLAEHSPYFEAMKQKDMEVLFCFEQFDELTLLHLREFDRKKLISAETDIVVDHYKEEKFQDSKPASERLSSEQAEDLLAWMRNALVQRVTNIKVTPRLDTHPAMITVLEMGAARHFLRTQQLARSSEERAQILQPTLEINTGHDLIKKLHALKDSNPELAQLLLEQIYDNAMIAAGLNEDPRPMISRLNQLLTRALEKH

Target ORF information:

RefSeq Version	NM_001113625.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio TNF receptor-associated protein 1 (trap1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001113625.1

ORF Insert Sequence:

ATGATCCGAT CGCTGAATTT ACTGAAATAT TCTCTAAATG TGTCCCAGCG GGCTCAGCTC 
AGGCTGCTCT CATCCAGCAA ATGGACAGGA CACAGGATTG TGCCTGCCGC GTTCACTGGA 
GTTGACACAA ATGGCAGACA GCAGCTGCAT CAGCCCAGAC CCTTCAGACT GTGGAGCCCC 
AGCTGTTCAG GGGTCAGTGT TCATCATCGC AGCTACAGCA CACAGCAACA CACAGAGCCT 
GCAGAGGAGG AGACGCTCCA CAACATCATC ACCGACACCG AGAACGTTCA AGGATCCTTC 
TCCAAACACG AATTTCAAGC AGAGACAAAG AAACTGCTGG ATATTGTGGC CAGATCGCTG 
TATTCTGAGA AAGAGGTGTT CATCAGGGAG CTGATCTCTA ACGGCAGTGA TGCTCTGGAG 
AAACTGCGGC ACAGGATGAT CACGGCTGGA GGAGACACAG CACCAATGGA GATCCACCTG 
CAGACGGACA GTGTTAAAGG CACCTTCACC ATACAGGACA CTGGTGTGGG GATGAACAAA 
GAGGATCTGG TTTCTAACCT GGGCACGATT GCTCGATCTG GATCAAAGGC TTTTCTGGAC 
GCTCTGCAGA ATCAAGCGGA GGCCAGCAGC TCCATCATTG GTCAGTTTGG TGTGGGCTTT 
TACTCCGCCT TCATGGTGGC CGATAAGGTG GAGGTTTATT CTCAGTCGGC AGAAGCAGAT 
GCTCCTGGAT ACAAGTGGTC CTCAGACGGC TCTGGAGTGT TTGAGGTCGC TGAAGCTTCA 
GGTGTTCGAC AGGGAACCAA GATTGTGCTC CACCTTAAAG ACGACTGCAA AGAGTTTTCA 
TCAGAGGACA GAGTTAAAGA GGTGGTGACC AAGTACAGCA ACTTTGTGAG TTTCCCCATC 
TTCCTGAATG GACGGAGGCT CAACACCCTG CAGGCACTCT GGATGATGGA GCCCAAAGAC 
ATCAGCGAGT GGCAGCACGA GGAGTTTTAT CGATATGTGG CTCAAGCCTA CGATAAACCG 
CGATACACAC TCCACTACCG CGCAGACGCT CCGCTCAACA TCCGCAGTAT CTTCTACGTG 
CCAGAAATGA AGCCGTCCAT GTTCGATGTG AGTCGTGAGA TGGGCTCCAG TGTGGCTCTG 
TACAGCAGAA AAATCCTGAT CCAAACCAAA GCCACAGACA TCTTGCCCAA GTGGCTGCGC 
TTCCTACGAG GTGTTGTGGA CAGTGAGGAT ATTCCTCTAA ACCTGAGCAG AGAGCTGCTA 
CAGGAAAGCG CCCTAATCAG GAAACTGCGG GATGTTTTAC AGCAGCGTGT CATTCGATTC 
CTGTTGGACC AGAGTAAGAA GGACCCGGAG AAATACGCTC GCTTCTTCGA GGACTACGGC 
CTGTTCATGC GCGAGGGCAT CGTGACCACA GGCGAGCAGA GCGTCAAGGA GGACATTGCT 
AAACTGCTGC GCTTCGAGTC GTCAGCTCTA CCTGCGGGAC AGCAGACCAG CCTGATGGAG 
TATTCCTCCC GCATGAAGGC AGGAACCAGG AACATTTATT ACCTGTGTGC GCCGAATCGA 
CACCTGGCCG AACACTCGCC GTACTTCGAG GCCATGAAAC AGAAAGATAT GGAGGTCTTG 
TTCTGCTTTG AGCAGTTTGA CGAGCTCACG CTTCTTCACC TCCGAGAGTT CGACAGGAAG 
AAGCTGATCT CCGCTGAGAC GGACATCGTT GTAGATCACT ACAAAGAGGA GAAATTCCAG 
GACAGCAAGC CAGCATCTGA GCGGTTGAGC AGTGAACAGG CTGAAGATCT GCTGGCCTGG 
ATGAGGAATG CACTCGTTCA GAGAGTCACC AACATCAAGG TGACTCCTCG TCTGGACACT 
CACCCGGCCA TGATCACAGT GCTGGAGATG GGAGCCGCTC GACACTTTCT GCGCACACAG 
CAGCTGGCCC GCAGCTCCGA GGAGAGAGCC CAGATCCTGC AGCCCACGCT GGAGATCAAC 
ACTGGGCATG ATCTGATCAA GAAGCTTCAT GCGCTGAAGG ACTCAAACCC TGAGCTGGCA 
CAGCTTCTGC TCGAACAGAT TTACGACAAT GCCATGATCG CCGCTGGCCT GAACGAAGAC 
CCTCGTCCGA TGATCTCTCG ACTGAATCAG CTGTTGACGC GAGCGCTGGA GAAACACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Symmetry broken and rebroken during the ATP hydrolysis cycle of the mitochondrial Hsp90 TRAP1.
eLife6(2017 Jul)
Elnatan D,Betegon M,Liu Y,Ramelot T,Kennedy MA,Agard DA

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 Mar)
Bay?s ?,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

Comparison of PBDE congeners as inducers of oxidative stress in zebrafish.
Environmental toxicology and chemistry34(5)1154-60(2015 May)
Usenko CY,Abel EL,Kudela M,Janise A,Bruce ED

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trap1 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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