kdm2aa cDNA ORF clone, Danio rerio(zebrafish)

The following kdm2aa gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the kdm2aa cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa33139 NM_001081692.2
Latest version!
Danio rerio lysine (K)-specific demethylase 2Aa (kdm2aa), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
ODa06033 XM_005160248.4
Latest version!
Danio rerio lysine (K)-specific demethylase 2Aa (kdm2aa), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
ODa46193 XM_009293357.3
Latest version!
Danio rerio lysine (K)-specific demethylase 2Aa (kdm2aa), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
ODa46194 XM_009293362.3
Latest version!
Danio rerio lysine (K)-specific demethylase 2Aa (kdm2aa), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
ODa46195 XM_009293365.3
Latest version!
Danio rerio lysine (K)-specific demethylase 2Aa (kdm2aa), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
View Old Accession Versions >>
ODa46193 XM_009293357.2 Danio rerio lysine (K)-specific demethylase 2Aa (kdm2aa), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
ODa46194 XM_009293362.2 Danio rerio lysine (K)-specific demethylase 2Aa (kdm2aa), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
ODa46195 XM_009293365.2 Danio rerio lysine (K)-specific demethylase 2Aa (kdm2aa), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
ODa06033 XM_005160248.3 Danio rerio lysine (K)-specific demethylase 2Aa (kdm2aa), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa33139
    Clone ID Related Accession (Same CDS sequence) NM_001081692.2
    Accession Version NM_001081692.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3888bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-06-29
    Organism Danio rerio(zebrafish)
    Product lysine (K)-specific demethylase 2B
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX901937.7. On or before Oct 14, 2011 this sequence version replaced NM_001080056.1, NM_001081692.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGACGTGA TGTCCGCTGG CGATGCGACG TCAAGATCTA AGCGGTCTGG CACGCGGAGG 
    CGTTACCAAG ACGATGGCAT TTCCGACGAC GAGATCGATG GAAGGAGGAC TTTCGATCTG
    GAAGAGAAGC TACACAGCAG CTTCTTCAGC TCAGACCGAG TCAAACGCAT GGAGGGCAAA
    GATTTCTCTT ATGAGTACGT CCAGAGGCAT GGTCTCCGGG ATCCTATCAT CTTTGAGAAA
    CCCGAAGGTC TTGGCATCAA AATGCCAGAT CCAGACTTCA CAGTAAATGA TGTGAAGTTG
    TTTGTAGGAA GTCGACGGAT AGTTGACGTG ATGGACGTGG CTACTCAGAA AGGGACGGAG
    ATGTCGATGG CCCAGTGGAG GCGCTACTAC GAGACTCCTC CCTCTCAGAG AGAAAAGCTT
    TATAATGTAA TCAGCCTTGA GTTTAGCCAC ACTAAACTAG AGACCCTTGT CAAAAGACCC
    TCCACGGTGG ACATGATTGA CTGGGTGGAC AACATGTGGC CTCGACACTT AAAGGAAAGA
    CAAAGAGATT CTACAAACTC TATCATGGAG ATGCAATACC CCAAAGTGCA AAAGTACTGC
    CTGATGAGTG TGCAGGGCTG TTACACAGAT TTCCACATAG ACTTTGGAGG CACGTCTGTG
    TGGTACCACA TACATAAAGG CTGCAAGGTG TTTTGGTTAA TCCCTCCCAC GCCTCAAAAC
    CTGGAGCTTT ATGAGAACTG GGTGTTGTCA GGGAAACAAG GCGACATCTT TCTTGGTGAC
    AGGGCCACTG ATTGCCAGAG GATTGAGCTC AAGCAGGGCT ACACGTTCAT CATTCCATCA
    GGCTGGATCC ATGCTGTGTA CACACCAGTG GACACGCTTG TCTTTGGTGG GAATTTTCTT
    CATAGTTTTA ACGTCCCCAT GCAATTGGAC ATCTCCGGCA TTGAAGACAG GACACGGGTG
    CCTGTGAAGT TCCGCTATCC GTTCTTCTTT GAGATGTGCT GGTATGTGTT GGAGCGTTAT
    CTCTACAGCT TGACCAACAA ATCCCACCTC ATCCCGGAGT TCCAGAAACA CTCACTGGGC
    ATTGGCTTGA AGCGGGAAAA CTCTGAGGTG CTGAATGGGC ATGCAAAAGA GGAAAGAGAT
    GAGATGGATG CCCCCTCTCC CCCTAGTGCA CCAGGGTTAA AGACTCACTT GACTGCTTTC
    GAGCTGGAAG GCCTGTGGAG GCTGGTAGGG AAGCTGGAAT CGCTGCCCTC AAATAAGAAG
    TGTGTGCCTT CAGGAATACA CAATGCTGCT GCCCTGCTGC TGGACATACG GGCATTGTTA
    AAAGAACATG CAAATGACAT CCCTAAGCTC TCTTACACTG GAAGACCCAT TGTCAGGTGG
    CCAAAGAGGC CTCCCTGGTA CCAGCCCCCT CCTCCGCCTC CACCTGTCAT GCGTCCCAAG
    CTGGCGACCA CACCTATTGT CCCACGGCCT GTCAAACCAG CCTCCAACAT GTCGGTCCTG
    CGCCGGCGCC GCGTGCGTTG CAAACGCTGC GAGGCCTGTC TGCGCACAGA ATGTGGCGAC
    TGCAACTTCT GCCGAGACAT GAAGAAATTT GGCGGCCCTG GCAAGCTCAA ACAGACCTGC
    GTCCTGCGGC AATGTCTTGC TCCTGGTCTG CCGCTCTCTG CTGTGTGTGA GCTCTGTGGC
    GAAGGGAATC AGGAGACCAG TGAAGAGCTG ATGGAGTGTT CCAACTGTGC TCAGATTGCA
    CATCCCAGCT GTTTGAAGAC GTCTGGTGAG GGAGTGGTGA ACAAAGACCT GCCGAGCTGC
    TGGGAGTGTC CCAAGTGTGT TTTGGGAAAA GACACCGATT CTGAGTCGTC AGGCAGCGGT
    GATGACATCA CGGCACAGGA TGGGTCAGGG GGGCGAGGGG GCGAGGGCGG GGCGTGGCAT
    GGGGTCGGGC GGCCCCCCTC TTCTCTCTCT CACGGCTCGC TCCAGAAACG GGCAGCAGCC
    CCTGAGCAGC GGCTCAGGAA GAGGATTAAA CTGGATGGTG GTAAATCACA TAAGCGCAAG
    TCTTCATCTC TTGATCCCCG CGTGGCTAAA ATCTACCGGA GACATGGGAT GGAGCACGAC
    GATGATTCAG GCAGCGATGA TGACGGAGAC GGTGGCAGAA TGTCTTCAGT GCGCAGCAGC
    GTTTCCTCAT CTCGCAGAGG TTATGGAGCT AGCAGGCGAG GCGTTTGGAG AGGCTCCTCA
    CAGCGGGGCC CTCGAGGGGG CGGAAGCCTG TCCTCAAGTT CCCTCAAAAT GAGGAGAGGA
    CTCGGAGCAA GAGGAGAGAG AGGAGGTCGG GTGAGGTTGC GAGGAGGATC GCAGATGAGG
    AGACGGCGGT ATGAAACTGA TGATGATGAT GATGACGACG ATGATGATGA TGATGACGAC
    GACGATGATG ATGACGAGGA TGAGGATGAT GAGGATGACA GCGAAGAAGA CAGGCATGTG
    AGCCGGTCAG AGAAGGAGCA TCGTTCCAGG AGACGAAGAC GAAGAGGTGA TGATTACGAT
    GATGATGAGG ACGAGGATGA CGATGATGAG GAGGACAGCG AAGAACAGGA CTATGAGGGA
    GAGGACGATG AAATGGAGGA CTTGGATGAT GGAGGAGATG AAGATGATGA GGATGGAGAA
    AACCAATCAG ACTCTGATCC TGATCCACCA GTTTTGCTCG TCTCAGACTT GAACGATGAC
    CTGCTGAACG ATTCCTACCT GACCGTGACC CTCCATCGGC CGCCGAAGGC CAAACGTGAC
    CCTGGCTCAA TTGTCCCTAA ACTAGAGGCG GCGATGTCCC CCCGCACGGG TGGCAGTTCT
    GGCTTCATGC AGCGAAAAAC TCTTCCCAAA GCGCGGCCCA AGACCAGCAG CTCCACCCCT
    GCTGGAAACG GCCTCTCTCA GGCTAAAAGC GGCCGTCCCA CGAGGCGAAG CTCCAACCAA
    GATGGCAGAG AGAGAGACAC GGCGTCGCCC TCCTCCATGT CGTCTCGCTC CTCTGTTTCT
    CCACCTCCTC CTCCTCCAGC TGTCACCTCT TCCTCTCCTT CCCTTCTCTC ACATCCTTCA
    TTCCGTGATG CAGGAAACGA GCTCGGCTGT GAAAAGGACA TTTGGGTGTC TGTGTTTCGT
    TACCTGGATC GGACAGATTT AGCTGTGTGC ATGAGAGTCT GCAAGGCTTG GTTTAAATGG
    TGTTGTGATA AACGCTTATG GACACGGATA GATCTGAGTC GCTGTCGGGC TCTCAGTCCT
    CAGGCTGTGA CGGCTATCAT TAAACTACAA CCTGTGACCC TTGACCTCTC CTGGACACCT
    GTGTCAAAGA AACAGCTGGC CTGGCTCATT CATCACCTCC CAAGTTTAAA AGATCTAATA
    ATGTCCGGTT GTTCTTCATT ATGTGTGTCT GCATTAAGCT CGCCGAGCTG TCCGAGTTTG
    CGAACTCTTG ACCTGTGTTG GGCTGTGGGC GTCAAAGACT CGCAGATAAA AGATCTTATT
    GTGCAGCCAG GCAGTGAAAG TCGCAGTCGT CTCAGAAGTT TGGTCAGTCT GCGCTTGTCT
    GGTTTGGAAT TGAGTGACGC TGTCATTAAG ACGATGGTCC GACACATGCC TTCACTGCGC
    CAGTTAGATC TGTCCTACTG TCAGGGCCTC ACAGACCAGT CCATCAACCT GCTCACTGCT
    ACTGGATGCA ACACTCGCAA CACACTCAGA CAGCTCAATC TTTCAGGTTG TAATAAGCTG
    TCAGATGGCT GTCTCTCATA TATGAAGCGA TTATCCGCTC TGGCTCTGTT GGATCTGCGG
    GGCTGCAAGA ACGTGACCCG ACATGGCTGC GAAAACTTCA TCTCGGACCT GTCCTACTGC
    ACCGTATTCT GCCTGACCGA GGACAAACTC ATTCAGAGAG TATCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001075161.2
    CDS207..4094
    Translation

    Target ORF information:

    RefSeq Version NM_001081692.2
    Organism Danio rerio(zebrafish)
    Definition Danio rerio lysine (K)-specific demethylase 2Aa (kdm2aa), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001081692.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGACGTGA TGTCCGCTGG CGATGCGACG TCAAGATCTA AGCGGTCTGG CACGCGGAGG 
    CGTTACCAAG ACGATGGCAT TTCCGACGAC GAGATCGATG GAAGGAGGAC TTTCGATCTG
    GAAGAGAAGC TACACAGCAG CTTCTTCAGC TCAGACCGAG TCAAACGCAT GGAGGGCAAA
    GATTTCTCTT ATGAGTACGT CCAGAGGCAT GGTCTCCGGG ATCCTATCAT CTTTGAGAAA
    CCCGAAGGTC TTGGCATCAA AATGCCAGAT CCAGACTTCA CAGTAAATGA TGTGAAGTTG
    TTTGTAGGAA GTCGACGGAT AGTTGACGTG ATGGACGTGG CTACTCAGAA AGGGACGGAG
    ATGTCGATGG CCCAGTGGAG GCGCTACTAC GAGACTCCTC CCTCTCAGAG AGAAAAGCTT
    TATAATGTAA TCAGCCTTGA GTTTAGCCAC ACTAAACTAG AGACCCTTGT CAAAAGACCC
    TCCACGGTGG ACATGATTGA CTGGGTGGAC AACATGTGGC CTCGACACTT AAAGGAAAGA
    CAAAGAGATT CTACAAACTC TATCATGGAG ATGCAATACC CCAAAGTGCA AAAGTACTGC
    CTGATGAGTG TGCAGGGCTG TTACACAGAT TTCCACATAG ACTTTGGAGG CACGTCTGTG
    TGGTACCACA TACATAAAGG CTGCAAGGTG TTTTGGTTAA TCCCTCCCAC GCCTCAAAAC
    CTGGAGCTTT ATGAGAACTG GGTGTTGTCA GGGAAACAAG GCGACATCTT TCTTGGTGAC
    AGGGCCACTG ATTGCCAGAG GATTGAGCTC AAGCAGGGCT ACACGTTCAT CATTCCATCA
    GGCTGGATCC ATGCTGTGTA CACACCAGTG GACACGCTTG TCTTTGGTGG GAATTTTCTT
    CATAGTTTTA ACGTCCCCAT GCAATTGGAC ATCTCCGGCA TTGAAGACAG GACACGGGTG
    CCTGTGAAGT TCCGCTATCC GTTCTTCTTT GAGATGTGCT GGTATGTGTT GGAGCGTTAT
    CTCTACAGCT TGACCAACAA ATCCCACCTC ATCCCGGAGT TCCAGAAACA CTCACTGGGC
    ATTGGCTTGA AGCGGGAAAA CTCTGAGGTG CTGAATGGGC ATGCAAAAGA GGAAAGAGAT
    GAGATGGATG CCCCCTCTCC CCCTAGTGCA CCAGGGTTAA AGACTCACTT GACTGCTTTC
    GAGCTGGAAG GCCTGTGGAG GCTGGTAGGG AAGCTGGAAT CGCTGCCCTC AAATAAGAAG
    TGTGTGCCTT CAGGAATACA CAATGCTGCT GCCCTGCTGC TGGACATACG GGCATTGTTA
    AAAGAACATG CAAATGACAT CCCTAAGCTC TCTTACACTG GAAGACCCAT TGTCAGGTGG
    CCAAAGAGGC CTCCCTGGTA CCAGCCCCCT CCTCCGCCTC CACCTGTCAT GCGTCCCAAG
    CTGGCGACCA CACCTATTGT CCCACGGCCT GTCAAACCAG CCTCCAACAT GTCGGTCCTG
    CGCCGGCGCC GCGTGCGTTG CAAACGCTGC GAGGCCTGTC TGCGCACAGA ATGTGGCGAC
    TGCAACTTCT GCCGAGACAT GAAGAAATTT GGCGGCCCTG GCAAGCTCAA ACAGACCTGC
    GTCCTGCGGC AATGTCTTGC TCCTGGTCTG CCGCTCTCTG CTGTGTGTGA GCTCTGTGGC
    GAAGGGAATC AGGAGACCAG TGAAGAGCTG ATGGAGTGTT CCAACTGTGC TCAGATTGCA
    CATCCCAGCT GTTTGAAGAC GTCTGGTGAG GGAGTGGTGA ACAAAGACCT GCCGAGCTGC
    TGGGAGTGTC CCAAGTGTGT TTTGGGAAAA GACACCGATT CTGAGTCGTC AGGCAGCGGT
    GATGACATCA CGGCACAGGA TGGGTCAGGG GGGCGAGGGG GCGAGGGCGG GGCGTGGCAT
    GGGGTCGGGC GGCCCCCCTC TTCTCTCTCT CACGGCTCGC TCCAGAAACG GGCAGCAGCC
    CCTGAGCAGC GGCTCAGGAA GAGGATTAAA CTGGATGGTG GTAAATCACA TAAGCGCAAG
    TCTTCATCTC TTGATCCCCG CGTGGCTAAA ATCTACCGGA GACATGGGAT GGAGCACGAC
    GATGATTCAG GCAGCGATGA TGACGGAGAC GGTGGCAGAA TGTCTTCAGT GCGCAGCAGC
    GTTTCCTCAT CTCGCAGAGG TTATGGAGCT AGCAGGCGAG GCGTTTGGAG AGGCTCCTCA
    CAGCGGGGCC CTCGAGGGGG CGGAAGCCTG TCCTCAAGTT CCCTCAAAAT GAGGAGAGGA
    CTCGGAGCAA GAGGAGAGAG AGGAGGTCGG GTGAGGTTGC GAGGAGGATC GCAGATGAGG
    AGACGGCGGT ATGAAACTGA TGATGATGAT GATGACGACG ATGATGATGA TGATGACGAC
    GACGATGATG ATGACGAGGA TGAGGATGAT GAGGATGACA GCGAAGAAGA CAGGCATGTG
    AGCCGGTCAG AGAAGGAGCA TCGTTCCAGG AGACGAAGAC GAAGAGGTGA TGATTACGAT
    GATGATGAGG ACGAGGATGA CGATGATGAG GAGGACAGCG AAGAACAGGA CTATGAGGGA
    GAGGACGATG AAATGGAGGA CTTGGATGAT GGAGGAGATG AAGATGATGA GGATGGAGAA
    AACCAATCAG ACTCTGATCC TGATCCACCA GTTTTGCTCG TCTCAGACTT GAACGATGAC
    CTGCTGAACG ATTCCTACCT GACCGTGACC CTCCATCGGC CGCCGAAGGC CAAACGTGAC
    CCTGGCTCAA TTGTCCCTAA ACTAGAGGCG GCGATGTCCC CCCGCACGGG TGGCAGTTCT
    GGCTTCATGC AGCGAAAAAC TCTTCCCAAA GCGCGGCCCA AGACCAGCAG CTCCACCCCT
    GCTGGAAACG GCCTCTCTCA GGCTAAAAGC GGCCGTCCCA CGAGGCGAAG CTCCAACCAA
    GATGGCAGAG AGAGAGACAC GGCGTCGCCC TCCTCCATGT CGTCTCGCTC CTCTGTTTCT
    CCACCTCCTC CTCCTCCAGC TGTCACCTCT TCCTCTCCTT CCCTTCTCTC ACATCCTTCA
    TTCCGTGATG CAGGAAACGA GCTCGGCTGT GAAAAGGACA TTTGGGTGTC TGTGTTTCGT
    TACCTGGATC GGACAGATTT AGCTGTGTGC ATGAGAGTCT GCAAGGCTTG GTTTAAATGG
    TGTTGTGATA AACGCTTATG GACACGGATA GATCTGAGTC GCTGTCGGGC TCTCAGTCCT
    CAGGCTGTGA CGGCTATCAT TAAACTACAA CCTGTGACCC TTGACCTCTC CTGGACACCT
    GTGTCAAAGA AACAGCTGGC CTGGCTCATT CATCACCTCC CAAGTTTAAA AGATCTAATA
    ATGTCCGGTT GTTCTTCATT ATGTGTGTCT GCATTAAGCT CGCCGAGCTG TCCGAGTTTG
    CGAACTCTTG ACCTGTGTTG GGCTGTGGGC GTCAAAGACT CGCAGATAAA AGATCTTATT
    GTGCAGCCAG GCAGTGAAAG TCGCAGTCGT CTCAGAAGTT TGGTCAGTCT GCGCTTGTCT
    GGTTTGGAAT TGAGTGACGC TGTCATTAAG ACGATGGTCC GACACATGCC TTCACTGCGC
    CAGTTAGATC TGTCCTACTG TCAGGGCCTC ACAGACCAGT CCATCAACCT GCTCACTGCT
    ACTGGATGCA ACACTCGCAA CACACTCAGA CAGCTCAATC TTTCAGGTTG TAATAAGCTG
    TCAGATGGCT GTCTCTCATA TATGAAGCGA TTATCCGCTC TGGCTCTGTT GGATCTGCGG
    GGCTGCAAGA ACGTGACCCG ACATGGCTGC GAAAACTTCA TCTCGGACCT GTCCTACTGC
    ACCGTATTCT GCCTGACCGA GGACAAACTC ATTCAGAGAG TATCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa06033
    Clone ID Related Accession (Same CDS sequence) XM_005160248.3 , XM_005160248.4
    Accession Version XM_005160248.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3957bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product lysine (K)-specific demethylase 2B isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005160248.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGAGAACG GACAGGAGGT GCTTACGCTT GTTGACGGGG GGATGTTGCT GATGAGACGG 
    AGAGGCAGGA AGAGGAAAAG CCAGCAGGGG GATGAAGATG TGACACTGAA GCGGTCTGGC
    ACGCGGAGGC GTTACCAAGA CGATGGCATT TCCGACGACG AGATCGATGG AAGGAGGACT
    TTCGATCTGG AAGAGAAGCT ACACAGCAGC TTCTTCAGCT CAGACCGAGT CAAACGCATG
    GAGGGCAAAG ATTTCTCTTA TGAGTACGTC CAGAGGCATG GTCTCCGGGA TCCTATCATC
    TTTGAGAAAC CCGAAGGTCT TGGCATCAAA ATGCCAGATC CAGACTTCAC AGTAAATGAT
    GTGAAGTTGT TTGTAGGAAG TCGACGGATA GTTGACGTGA TGGACGTGGC TACTCAGAAA
    GGGACGGAGA TGTCGATGGC CCAGTGGAGG CGCTACTACG AGACTCCTCC CTCTCAGAGA
    GAAAAGCTTT ATAATGTAAT CAGCCTTGAG TTTAGCCACA CTAAACTAGA GACCCTTGTC
    AAAAGACCCT CCACGGTGGA CATGATTGAC TGGGTGGACA ACATGTGGCC TCGACACTTA
    AAGGAAAGAC AAAGAGATTC TACAAACTCT ATCATGGAGA TGCAATACCC CAAAGTGCAA
    AAGTACTGCC TGATGAGTGT GCAGGGCTGT TACACAGATT TCCACATAGA CTTTGGAGGC
    ACGTCTGTGT GGTACCACAT ACATAAAGGC TGCAAGGTGT TTTGGTTAAT CCCTCCCACG
    CCTCAAAACC TGGAGCTTTA TGAGAACTGG GTGTTGTCAG GGAAACAAGG CGACATCTTT
    CTTGGTGACA GGGCCACTGA TTGCCAGAGG ATTGAGCTCA AGCAGGGCTA CACGTTCATC
    ATTCCATCAG GCTGGATCCA TGCTGTGTAC ACACCAGTGG ACACGCTTGT CTTTGGTGGG
    AATTTTCTTC ATAGTTTTAA CGTCCCCATG CAATTGGACA TCTCCGGCAT TGAAGACAGG
    ACACGGGTGC CTGTGAAGTT CCGCTATCCG TTCTTCTTTG AGATGTGCTG GTATGTGTTG
    GAGCGTTATC TCTACAGCTT GACCAACAAA TCCCACCTCA TCCCGGAGTT CCAGAAACAC
    TCACTGGGCA TTGGCTTGAA GCGGGAAAAC TCTGAGGTGC TGAATGGGCA TGCAAAAGAG
    GAAAGAGATG AGATGGATGC CCCCTCTCCC CCTAGTGCAC CAGGGTTAAA GACTCACTTG
    ACTGCTTTCG AGCTGGAAGG CCTGTGGAGG CTGGTAGGGA AGCTGGAATC GCTGCCCTCA
    AATAAGAAGT GTGTGCCTTC AGGAATACAC AATGCTGCTG CCCTGCTGCT GGACATACGG
    GCATTGTTAA AAGAACATGC AAATGACATC CCTAAGCTCT CTTACACTGG AAGACCCATT
    GTCAGGTGGC CAAAGAGGCC TCCCTGGTAC CAGCCCCCTC CTCCGCCTCC ACCTGTCATG
    CGTCCCAAGC TGGCGACCAC ACCTATTGTC CCACGGCCTG TCAAACCAGC CTCCAACATG
    TCGGTCCTGC GCCGGCGCCG CGTGCGTTGC AAACGCTGCG AGGCCTGTCT GCGCACAGAA
    TGTGGCGACT GCAACTTCTG CCGAGACATG AAGAAATTTG GCGGCCCTGG CAAGCTCAAA
    CAGACCTGCG TCCTGCGGCA ATGTCTTGCT CCTGGTCTGC CGCTCTCTGC TGTGTGTGAG
    CTCTGTGGCG AAGGGAATCA GGAGACCAGT GAAGAGCTGA TGGAGTGTTC CAACTGTGCT
    CAGATTGCAC ATCCCAGCTG TTTGAAGACG TCTGGTGAGG GAGTGGTGAA CAAAGACCTG
    CCGAGCTGCT GGGAGTGTCC CAAGTGTGTT TTGGGAAAAG ACACCGATTC TGAGTCGTCA
    GGCAGCGGTG ATGACATCAC GGCACAGGAT GGGTCAGGGG GGCGAGGGGG CGAGGGCGGG
    GCGTGGCATG GGGTCGGGCG GCCCCCCTCT TCTCTCTCTC ACGGCTCGCT CCAGAAACGG
    GCAGCAGCCC CTGAGCAGCG GCTCAGGAAG AGGATTAAAC TGGATGGTGG TAAATCACAT
    AAGCGCAAGT CTTCATCTCT TGATCCCCGC GTGGCTAAAA TCTACCGGAG ACATGGGATG
    GAGCACGACG ATGATTCAGG CAGCGATGAT GACGGAGACG GTGGCAGAAT GTCTTCAGTG
    CGCAGCAGCG TTTCCTCATC TCGCAGAGGT TATGGAGCTA GCAGGCGAGG CGTTTGGAGA
    GGCTCCTCAC AGCGGGGCCC TCGAGGGGGC GGAAGCCTGT CCTCAAGTTC CCTCAAAATG
    AGGAGAGGAC TCGGAGCAAG AGGAGAGAGA GGAGGTCGGG TGAGGTTGCG AGGAGGATCG
    CAGATGAGGA GACGGCGGTA TGAAACTGAT GATGATGATG ATGACGACGA TGATGATGAT
    GATGACGACG ACGATGATGA TGACGAGGAT GAGGATGATG AGGATGACAG CGAAGAAGAC
    AGGCATGTGA GCCGGTCAGA GAAGGAGCAT CGTTCCAGGA GACGAAGACG AAGAGGTGAT
    GATTACGATG ATGATGAGGA CGAGGATGAC GATGATGAGG AGGACAGCGA AGAACAGGAC
    TATGAGGGAG AGGACGATGA AATGGAGGAC TTGGATGATG GAGGAGATGA AGATGATGAG
    GATGGAGAAA ACCAATCAGA CTCTGATCCT GATCCACCAG TTTTGCTCGT CTCAGACTTG
    AACGATGACC TGCTGAACGA TTCCTACCTG ACCGTGACCC TCCATCGGCC GCCGAAGGCC
    AAACGTGACC CTGGCTCAAT TGTCCCTAAA CTAGAGGCGG CGATGTCCCC CCGCACGGGT
    GGCAGTTCTG GCTTCATGCA GCGAAAAACT CTTCCCAAAG CGCGGCCCAA GACCAGCAGC
    TCCACCCCTG CTGGAAACGG CCTCTCTCAG GCTAAAAGCG GCCGTCCCAC GAGGCGAAGC
    TCCAACCAAG ATGGCAGAGA GAGAGACACG GCGTCGCCCT CCTCCATGTC GTCTCGCTCC
    TCTGTTTCTC CACCTCCTCC TCCTCCAGCT GTCACCTCTT CCTCTCCTTC CCTTCTCTCA
    CATCCTTCAT TCCGTGATGC AGGAAACGAG CTCGGCTGTG AAAAGGACAT TTGGGTGTCT
    GTGTTTCGTT ACCTGGATCG GACAGATTTA GCTGTGTGCA TGAGAGTCTG CAAGGCTTGG
    TTTAAATGGT GTTGTGATAA ACGCTTATGG ACACGGATAG ATCTGAGTCG CTGTCGGGCT
    CTCAGTCCTC AGGCTGTGAC GGCTATCATT AAACTACAAC CTGTGACCCT TGACCTCTCC
    TGGACACCTG TGTCAAAGAA ACAGCTGGCC TGGCTCATTC ATCACCTCCC AAGTTTAAAA
    GATCTAATAA TGTCCGGTTG TTCTTCATTA TGTGTGTCTG CATTAAGCTC GCCGAGCTGT
    CCGAGTTTGC GAACTCTTGA CCTGTGTTGG GCTGTGGGCG TCAAAGACTC GCAGATAAAA
    GATCTTATTG TGCAGCCAGG CAGTGAAAGT CGCAGTCGTC TCAGAAGTTT GGTCAGTCTG
    CGCTTGTCTG GTTTGGAATT GAGTGACGCT GTCATTAAGA CGATGGTCCG ACACATGCCT
    TCACTGCGCC AGTTAGATCT GTCCTACTGT CAGGGCCTCA CAGACCAGTC CATCAACCTG
    CTCACTGCTA CTGGATGCAA CACTCGCAAC ACACTCAGAC AGCTCAATCT TTCAGGTTGT
    AATAAGCTGT CAGATGGCTG TCTCTCATAT ATGAAGCGAT TATCCGCTCT GGCTCTGTTG
    GATCTGCGGG GCTGCAAGAA CGTGACCCGA CATGGCTGCG AAAACTTCAT CTCGGACCTG
    TCCTACTGCA CCGTATTCTG CCTGACCGAG GACAAACTCA TTCAGAGAGT ATCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005160305.1
    CDS238..4194
    Translation

    Target ORF information:

    RefSeq Version XM_005160248.3
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio lysine (K)-specific demethylase 2Aa (kdm2aa), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005160248.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGAGAACG GACAGGAGGT GCTTACGCTT GTTGACGGGG GGATGTTGCT GATGAGACGG 
    AGAGGCAGGA AGAGGAAAAG CCAGCAGGGG GATGAAGATG TGACACTGAA GCGGTCTGGC
    ACGCGGAGGC GTTACCAAGA CGATGGCATT TCCGACGACG AGATCGATGG AAGGAGGACT
    TTCGATCTGG AAGAGAAGCT ACACAGCAGC TTCTTCAGCT CAGACCGAGT CAAACGCATG
    GAGGGCAAAG ATTTCTCTTA TGAGTACGTC CAGAGGCATG GTCTCCGGGA TCCTATCATC
    TTTGAGAAAC CCGAAGGTCT TGGCATCAAA ATGCCAGATC CAGACTTCAC AGTAAATGAT
    GTGAAGTTGT TTGTAGGAAG TCGACGGATA GTTGACGTGA TGGACGTGGC TACTCAGAAA
    GGGACGGAGA TGTCGATGGC CCAGTGGAGG CGCTACTACG AGACTCCTCC CTCTCAGAGA
    GAAAAGCTTT ATAATGTAAT CAGCCTTGAG TTTAGCCACA CTAAACTAGA GACCCTTGTC
    AAAAGACCCT CCACGGTGGA CATGATTGAC TGGGTGGACA ACATGTGGCC TCGACACTTA
    AAGGAAAGAC AAAGAGATTC TACAAACTCT ATCATGGAGA TGCAATACCC CAAAGTGCAA
    AAGTACTGCC TGATGAGTGT GCAGGGCTGT TACACAGATT TCCACATAGA CTTTGGAGGC
    ACGTCTGTGT GGTACCACAT ACATAAAGGC TGCAAGGTGT TTTGGTTAAT CCCTCCCACG
    CCTCAAAACC TGGAGCTTTA TGAGAACTGG GTGTTGTCAG GGAAACAAGG CGACATCTTT
    CTTGGTGACA GGGCCACTGA TTGCCAGAGG ATTGAGCTCA AGCAGGGCTA CACGTTCATC
    ATTCCATCAG GCTGGATCCA TGCTGTGTAC ACACCAGTGG ACACGCTTGT CTTTGGTGGG
    AATTTTCTTC ATAGTTTTAA CGTCCCCATG CAATTGGACA TCTCCGGCAT TGAAGACAGG
    ACACGGGTGC CTGTGAAGTT CCGCTATCCG TTCTTCTTTG AGATGTGCTG GTATGTGTTG
    GAGCGTTATC TCTACAGCTT GACCAACAAA TCCCACCTCA TCCCGGAGTT CCAGAAACAC
    TCACTGGGCA TTGGCTTGAA GCGGGAAAAC TCTGAGGTGC TGAATGGGCA TGCAAAAGAG
    GAAAGAGATG AGATGGATGC CCCCTCTCCC CCTAGTGCAC CAGGGTTAAA GACTCACTTG
    ACTGCTTTCG AGCTGGAAGG CCTGTGGAGG CTGGTAGGGA AGCTGGAATC GCTGCCCTCA
    AATAAGAAGT GTGTGCCTTC AGGAATACAC AATGCTGCTG CCCTGCTGCT GGACATACGG
    GCATTGTTAA AAGAACATGC AAATGACATC CCTAAGCTCT CTTACACTGG AAGACCCATT
    GTCAGGTGGC CAAAGAGGCC TCCCTGGTAC CAGCCCCCTC CTCCGCCTCC ACCTGTCATG
    CGTCCCAAGC TGGCGACCAC ACCTATTGTC CCACGGCCTG TCAAACCAGC CTCCAACATG
    TCGGTCCTGC GCCGGCGCCG CGTGCGTTGC AAACGCTGCG AGGCCTGTCT GCGCACAGAA
    TGTGGCGACT GCAACTTCTG CCGAGACATG AAGAAATTTG GCGGCCCTGG CAAGCTCAAA
    CAGACCTGCG TCCTGCGGCA ATGTCTTGCT CCTGGTCTGC CGCTCTCTGC TGTGTGTGAG
    CTCTGTGGCG AAGGGAATCA GGAGACCAGT GAAGAGCTGA TGGAGTGTTC CAACTGTGCT
    CAGATTGCAC ATCCCAGCTG TTTGAAGACG TCTGGTGAGG GAGTGGTGAA CAAAGACCTG
    CCGAGCTGCT GGGAGTGTCC CAAGTGTGTT TTGGGAAAAG ACACCGATTC TGAGTCGTCA
    GGCAGCGGTG ATGACATCAC GGCACAGGAT GGGTCAGGGG GGCGAGGGGG CGAGGGCGGG
    GCGTGGCATG GGGTCGGGCG GCCCCCCTCT TCTCTCTCTC ACGGCTCGCT CCAGAAACGG
    GCAGCAGCCC CTGAGCAGCG GCTCAGGAAG AGGATTAAAC TGGATGGTGG TAAATCACAT
    AAGCGCAAGT CTTCATCTCT TGATCCCCGC GTGGCTAAAA TCTACCGGAG ACATGGGATG
    GAGCACGACG ATGATTCAGG CAGCGATGAT GACGGAGACG GTGGCAGAAT GTCTTCAGTG
    CGCAGCAGCG TTTCCTCATC TCGCAGAGGT TATGGAGCTA GCAGGCGAGG CGTTTGGAGA
    GGCTCCTCAC AGCGGGGCCC TCGAGGGGGC GGAAGCCTGT CCTCAAGTTC CCTCAAAATG
    AGGAGAGGAC TCGGAGCAAG AGGAGAGAGA GGAGGTCGGG TGAGGTTGCG AGGAGGATCG
    CAGATGAGGA GACGGCGGTA TGAAACTGAT GATGATGATG ATGACGACGA TGATGATGAT
    GATGACGACG ACGATGATGA TGACGAGGAT GAGGATGATG AGGATGACAG CGAAGAAGAC
    AGGCATGTGA GCCGGTCAGA GAAGGAGCAT CGTTCCAGGA GACGAAGACG AAGAGGTGAT
    GATTACGATG ATGATGAGGA CGAGGATGAC GATGATGAGG AGGACAGCGA AGAACAGGAC
    TATGAGGGAG AGGACGATGA AATGGAGGAC TTGGATGATG GAGGAGATGA AGATGATGAG
    GATGGAGAAA ACCAATCAGA CTCTGATCCT GATCCACCAG TTTTGCTCGT CTCAGACTTG
    AACGATGACC TGCTGAACGA TTCCTACCTG ACCGTGACCC TCCATCGGCC GCCGAAGGCC
    AAACGTGACC CTGGCTCAAT TGTCCCTAAA CTAGAGGCGG CGATGTCCCC CCGCACGGGT
    GGCAGTTCTG GCTTCATGCA GCGAAAAACT CTTCCCAAAG CGCGGCCCAA GACCAGCAGC
    TCCACCCCTG CTGGAAACGG CCTCTCTCAG GCTAAAAGCG GCCGTCCCAC GAGGCGAAGC
    TCCAACCAAG ATGGCAGAGA GAGAGACACG GCGTCGCCCT CCTCCATGTC GTCTCGCTCC
    TCTGTTTCTC CACCTCCTCC TCCTCCAGCT GTCACCTCTT CCTCTCCTTC CCTTCTCTCA
    CATCCTTCAT TCCGTGATGC AGGAAACGAG CTCGGCTGTG AAAAGGACAT TTGGGTGTCT
    GTGTTTCGTT ACCTGGATCG GACAGATTTA GCTGTGTGCA TGAGAGTCTG CAAGGCTTGG
    TTTAAATGGT GTTGTGATAA ACGCTTATGG ACACGGATAG ATCTGAGTCG CTGTCGGGCT
    CTCAGTCCTC AGGCTGTGAC GGCTATCATT AAACTACAAC CTGTGACCCT TGACCTCTCC
    TGGACACCTG TGTCAAAGAA ACAGCTGGCC TGGCTCATTC ATCACCTCCC AAGTTTAAAA
    GATCTAATAA TGTCCGGTTG TTCTTCATTA TGTGTGTCTG CATTAAGCTC GCCGAGCTGT
    CCGAGTTTGC GAACTCTTGA CCTGTGTTGG GCTGTGGGCG TCAAAGACTC GCAGATAAAA
    GATCTTATTG TGCAGCCAGG CAGTGAAAGT CGCAGTCGTC TCAGAAGTTT GGTCAGTCTG
    CGCTTGTCTG GTTTGGAATT GAGTGACGCT GTCATTAAGA CGATGGTCCG ACACATGCCT
    TCACTGCGCC AGTTAGATCT GTCCTACTGT CAGGGCCTCA CAGACCAGTC CATCAACCTG
    CTCACTGCTA CTGGATGCAA CACTCGCAAC ACACTCAGAC AGCTCAATCT TTCAGGTTGT
    AATAAGCTGT CAGATGGCTG TCTCTCATAT ATGAAGCGAT TATCCGCTCT GGCTCTGTTG
    GATCTGCGGG GCTGCAAGAA CGTGACCCGA CATGGCTGCG AAAACTTCAT CTCGGACCTG
    TCCTACTGCA CCGTATTCTG CCTGACCGAG GACAAACTCA TTCAGAGAGT ATCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa06033
    Clone ID Related Accession (Same CDS sequence) XM_005160248.3 , XM_005160248.4
    Accession Version XM_005160248.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3957bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product lysine (K)-specific demethylase 2B isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005160248.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGAGAACG GACAGGAGGT GCTTACGCTT GTTGACGGGG GGATGTTGCT GATGAGACGG 
    AGAGGCAGGA AGAGGAAAAG CCAGCAGGGG GATGAAGATG TGACACTGAA GCGGTCTGGC
    ACGCGGAGGC GTTACCAAGA CGATGGCATT TCCGACGACG AGATCGATGG AAGGAGGACT
    TTCGATCTGG AAGAGAAGCT ACACAGCAGC TTCTTCAGCT CAGACCGAGT CAAACGCATG
    GAGGGCAAAG ATTTCTCTTA TGAGTACGTC CAGAGGCATG GTCTCCGGGA TCCTATCATC
    TTTGAGAAAC CCGAAGGTCT TGGCATCAAA ATGCCAGATC CAGACTTCAC AGTAAATGAT
    GTGAAGTTGT TTGTAGGAAG TCGACGGATA GTTGACGTGA TGGACGTGGC TACTCAGAAA
    GGGACGGAGA TGTCGATGGC CCAGTGGAGG CGCTACTACG AGACTCCTCC CTCTCAGAGA
    GAAAAGCTTT ATAATGTAAT CAGCCTTGAG TTTAGCCACA CTAAACTAGA GACCCTTGTC
    AAAAGACCCT CCACGGTGGA CATGATTGAC TGGGTGGACA ACATGTGGCC TCGACACTTA
    AAGGAAAGAC AAAGAGATTC TACAAACTCT ATCATGGAGA TGCAATACCC CAAAGTGCAA
    AAGTACTGCC TGATGAGTGT GCAGGGCTGT TACACAGATT TCCACATAGA CTTTGGAGGC
    ACGTCTGTGT GGTACCACAT ACATAAAGGC TGCAAGGTGT TTTGGTTAAT CCCTCCCACG
    CCTCAAAACC TGGAGCTTTA TGAGAACTGG GTGTTGTCAG GGAAACAAGG CGACATCTTT
    CTTGGTGACA GGGCCACTGA TTGCCAGAGG ATTGAGCTCA AGCAGGGCTA CACGTTCATC
    ATTCCATCAG GCTGGATCCA TGCTGTGTAC ACACCAGTGG ACACGCTTGT CTTTGGTGGG
    AATTTTCTTC ATAGTTTTAA CGTCCCCATG CAATTGGACA TCTCCGGCAT TGAAGACAGG
    ACACGGGTGC CTGTGAAGTT CCGCTATCCG TTCTTCTTTG AGATGTGCTG GTATGTGTTG
    GAGCGTTATC TCTACAGCTT GACCAACAAA TCCCACCTCA TCCCGGAGTT CCAGAAACAC
    TCACTGGGCA TTGGCTTGAA GCGGGAAAAC TCTGAGGTGC TGAATGGGCA TGCAAAAGAG
    GAAAGAGATG AGATGGATGC CCCCTCTCCC CCTAGTGCAC CAGGGTTAAA GACTCACTTG
    ACTGCTTTCG AGCTGGAAGG CCTGTGGAGG CTGGTAGGGA AGCTGGAATC GCTGCCCTCA
    AATAAGAAGT GTGTGCCTTC AGGAATACAC AATGCTGCTG CCCTGCTGCT GGACATACGG
    GCATTGTTAA AAGAACATGC AAATGACATC CCTAAGCTCT CTTACACTGG AAGACCCATT
    GTCAGGTGGC CAAAGAGGCC TCCCTGGTAC CAGCCCCCTC CTCCGCCTCC ACCTGTCATG
    CGTCCCAAGC TGGCGACCAC ACCTATTGTC CCACGGCCTG TCAAACCAGC CTCCAACATG
    TCGGTCCTGC GCCGGCGCCG CGTGCGTTGC AAACGCTGCG AGGCCTGTCT GCGCACAGAA
    TGTGGCGACT GCAACTTCTG CCGAGACATG AAGAAATTTG GCGGCCCTGG CAAGCTCAAA
    CAGACCTGCG TCCTGCGGCA ATGTCTTGCT CCTGGTCTGC CGCTCTCTGC TGTGTGTGAG
    CTCTGTGGCG AAGGGAATCA GGAGACCAGT GAAGAGCTGA TGGAGTGTTC CAACTGTGCT
    CAGATTGCAC ATCCCAGCTG TTTGAAGACG TCTGGTGAGG GAGTGGTGAA CAAAGACCTG
    CCGAGCTGCT GGGAGTGTCC CAAGTGTGTT TTGGGAAAAG ACACCGATTC TGAGTCGTCA
    GGCAGCGGTG ATGACATCAC GGCACAGGAT GGGTCAGGGG GGCGAGGGGG CGAGGGCGGG
    GCGTGGCATG GGGTCGGGCG GCCCCCCTCT TCTCTCTCTC ACGGCTCGCT CCAGAAACGG
    GCAGCAGCCC CTGAGCAGCG GCTCAGGAAG AGGATTAAAC TGGATGGTGG TAAATCACAT
    AAGCGCAAGT CTTCATCTCT TGATCCCCGC GTGGCTAAAA TCTACCGGAG ACATGGGATG
    GAGCACGACG ATGATTCAGG CAGCGATGAT GACGGAGACG GTGGCAGAAT GTCTTCAGTG
    CGCAGCAGCG TTTCCTCATC TCGCAGAGGT TATGGAGCTA GCAGGCGAGG CGTTTGGAGA
    GGCTCCTCAC AGCGGGGCCC TCGAGGGGGC GGAAGCCTGT CCTCAAGTTC CCTCAAAATG
    AGGAGAGGAC TCGGAGCAAG AGGAGAGAGA GGAGGTCGGG TGAGGTTGCG AGGAGGATCG
    CAGATGAGGA GACGGCGGTA TGAAACTGAT GATGATGATG ATGACGACGA TGATGATGAT
    GATGACGACG ACGATGATGA TGACGAGGAT GAGGATGATG AGGATGACAG CGAAGAAGAC
    AGGCATGTGA GCCGGTCAGA GAAGGAGCAT CGTTCCAGGA GACGAAGACG AAGAGGTGAT
    GATTACGATG ATGATGAGGA CGAGGATGAC GATGATGAGG AGGACAGCGA AGAACAGGAC
    TATGAGGGAG AGGACGATGA AATGGAGGAC TTGGATGATG GAGGAGATGA AGATGATGAG
    GATGGAGAAA ACCAATCAGA CTCTGATCCT GATCCACCAG TTTTGCTCGT CTCAGACTTG
    AACGATGACC TGCTGAACGA TTCCTACCTG ACCGTGACCC TCCATCGGCC GCCGAAGGCC
    AAACGTGACC CTGGCTCAAT TGTCCCTAAA CTAGAGGCGG CGATGTCCCC CCGCACGGGT
    GGCAGTTCTG GCTTCATGCA GCGAAAAACT CTTCCCAAAG CGCGGCCCAA GACCAGCAGC
    TCCACCCCTG CTGGAAACGG CCTCTCTCAG GCTAAAAGCG GCCGTCCCAC GAGGCGAAGC
    TCCAACCAAG ATGGCAGAGA GAGAGACACG GCGTCGCCCT CCTCCATGTC GTCTCGCTCC
    TCTGTTTCTC CACCTCCTCC TCCTCCAGCT GTCACCTCTT CCTCTCCTTC CCTTCTCTCA
    CATCCTTCAT TCCGTGATGC AGGAAACGAG CTCGGCTGTG AAAAGGACAT TTGGGTGTCT
    GTGTTTCGTT ACCTGGATCG GACAGATTTA GCTGTGTGCA TGAGAGTCTG CAAGGCTTGG
    TTTAAATGGT GTTGTGATAA ACGCTTATGG ACACGGATAG ATCTGAGTCG CTGTCGGGCT
    CTCAGTCCTC AGGCTGTGAC GGCTATCATT AAACTACAAC CTGTGACCCT TGACCTCTCC
    TGGACACCTG TGTCAAAGAA ACAGCTGGCC TGGCTCATTC ATCACCTCCC AAGTTTAAAA
    GATCTAATAA TGTCCGGTTG TTCTTCATTA TGTGTGTCTG CATTAAGCTC GCCGAGCTGT
    CCGAGTTTGC GAACTCTTGA CCTGTGTTGG GCTGTGGGCG TCAAAGACTC GCAGATAAAA
    GATCTTATTG TGCAGCCAGG CAGTGAAAGT CGCAGTCGTC TCAGAAGTTT GGTCAGTCTG
    CGCTTGTCTG GTTTGGAATT GAGTGACGCT GTCATTAAGA CGATGGTCCG ACACATGCCT
    TCACTGCGCC AGTTAGATCT GTCCTACTGT CAGGGCCTCA CAGACCAGTC CATCAACCTG
    CTCACTGCTA CTGGATGCAA CACTCGCAAC ACACTCAGAC AGCTCAATCT TTCAGGTTGT
    AATAAGCTGT CAGATGGCTG TCTCTCATAT ATGAAGCGAT TATCCGCTCT GGCTCTGTTG
    GATCTGCGGG GCTGCAAGAA CGTGACCCGA CATGGCTGCG AAAACTTCAT CTCGGACCTG
    TCCTACTGCA CCGTATTCTG CCTGACCGAG GACAAACTCA TTCAGAGAGT ATCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005160305.1
    CDS96..4052
    Translation

    Target ORF information:

    RefSeq Version XM_005160248.4
    Organism Danio rerio(zebrafish)
    Definition Danio rerio lysine (K)-specific demethylase 2Aa (kdm2aa), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005160248.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGAGAACG GACAGGAGGT GCTTACGCTT GTTGACGGGG GGATGTTGCT GATGAGACGG 
    AGAGGCAGGA AGAGGAAAAG CCAGCAGGGG GATGAAGATG TGACACTGAA GCGGTCTGGC
    ACGCGGAGGC GTTACCAAGA CGATGGCATT TCCGACGACG AGATCGATGG AAGGAGGACT
    TTCGATCTGG AAGAGAAGCT ACACAGCAGC TTCTTCAGCT CAGACCGAGT CAAACGCATG
    GAGGGCAAAG ATTTCTCTTA TGAGTACGTC CAGAGGCATG GTCTCCGGGA TCCTATCATC
    TTTGAGAAAC CCGAAGGTCT TGGCATCAAA ATGCCAGATC CAGACTTCAC AGTAAATGAT
    GTGAAGTTGT TTGTAGGAAG TCGACGGATA GTTGACGTGA TGGACGTGGC TACTCAGAAA
    GGGACGGAGA TGTCGATGGC CCAGTGGAGG CGCTACTACG AGACTCCTCC CTCTCAGAGA
    GAAAAGCTTT ATAATGTAAT CAGCCTTGAG TTTAGCCACA CTAAACTAGA GACCCTTGTC
    AAAAGACCCT CCACGGTGGA CATGATTGAC TGGGTGGACA ACATGTGGCC TCGACACTTA
    AAGGAAAGAC AAAGAGATTC TACAAACTCT ATCATGGAGA TGCAATACCC CAAAGTGCAA
    AAGTACTGCC TGATGAGTGT GCAGGGCTGT TACACAGATT TCCACATAGA CTTTGGAGGC
    ACGTCTGTGT GGTACCACAT ACATAAAGGC TGCAAGGTGT TTTGGTTAAT CCCTCCCACG
    CCTCAAAACC TGGAGCTTTA TGAGAACTGG GTGTTGTCAG GGAAACAAGG CGACATCTTT
    CTTGGTGACA GGGCCACTGA TTGCCAGAGG ATTGAGCTCA AGCAGGGCTA CACGTTCATC
    ATTCCATCAG GCTGGATCCA TGCTGTGTAC ACACCAGTGG ACACGCTTGT CTTTGGTGGG
    AATTTTCTTC ATAGTTTTAA CGTCCCCATG CAATTGGACA TCTCCGGCAT TGAAGACAGG
    ACACGGGTGC CTGTGAAGTT CCGCTATCCG TTCTTCTTTG AGATGTGCTG GTATGTGTTG
    GAGCGTTATC TCTACAGCTT GACCAACAAA TCCCACCTCA TCCCGGAGTT CCAGAAACAC
    TCACTGGGCA TTGGCTTGAA GCGGGAAAAC TCTGAGGTGC TGAATGGGCA TGCAAAAGAG
    GAAAGAGATG AGATGGATGC CCCCTCTCCC CCTAGTGCAC CAGGGTTAAA GACTCACTTG
    ACTGCTTTCG AGCTGGAAGG CCTGTGGAGG CTGGTAGGGA AGCTGGAATC GCTGCCCTCA
    AATAAGAAGT GTGTGCCTTC AGGAATACAC AATGCTGCTG CCCTGCTGCT GGACATACGG
    GCATTGTTAA AAGAACATGC AAATGACATC CCTAAGCTCT CTTACACTGG AAGACCCATT
    GTCAGGTGGC CAAAGAGGCC TCCCTGGTAC CAGCCCCCTC CTCCGCCTCC ACCTGTCATG
    CGTCCCAAGC TGGCGACCAC ACCTATTGTC CCACGGCCTG TCAAACCAGC CTCCAACATG
    TCGGTCCTGC GCCGGCGCCG CGTGCGTTGC AAACGCTGCG AGGCCTGTCT GCGCACAGAA
    TGTGGCGACT GCAACTTCTG CCGAGACATG AAGAAATTTG GCGGCCCTGG CAAGCTCAAA
    CAGACCTGCG TCCTGCGGCA ATGTCTTGCT CCTGGTCTGC CGCTCTCTGC TGTGTGTGAG
    CTCTGTGGCG AAGGGAATCA GGAGACCAGT GAAGAGCTGA TGGAGTGTTC CAACTGTGCT
    CAGATTGCAC ATCCCAGCTG TTTGAAGACG TCTGGTGAGG GAGTGGTGAA CAAAGACCTG
    CCGAGCTGCT GGGAGTGTCC CAAGTGTGTT TTGGGAAAAG ACACCGATTC TGAGTCGTCA
    GGCAGCGGTG ATGACATCAC GGCACAGGAT GGGTCAGGGG GGCGAGGGGG CGAGGGCGGG
    GCGTGGCATG GGGTCGGGCG GCCCCCCTCT TCTCTCTCTC ACGGCTCGCT CCAGAAACGG
    GCAGCAGCCC CTGAGCAGCG GCTCAGGAAG AGGATTAAAC TGGATGGTGG TAAATCACAT
    AAGCGCAAGT CTTCATCTCT TGATCCCCGC GTGGCTAAAA TCTACCGGAG ACATGGGATG
    GAGCACGACG ATGATTCAGG CAGCGATGAT GACGGAGACG GTGGCAGAAT GTCTTCAGTG
    CGCAGCAGCG TTTCCTCATC TCGCAGAGGT TATGGAGCTA GCAGGCGAGG CGTTTGGAGA
    GGCTCCTCAC AGCGGGGCCC TCGAGGGGGC GGAAGCCTGT CCTCAAGTTC CCTCAAAATG
    AGGAGAGGAC TCGGAGCAAG AGGAGAGAGA GGAGGTCGGG TGAGGTTGCG AGGAGGATCG
    CAGATGAGGA GACGGCGGTA TGAAACTGAT GATGATGATG ATGACGACGA TGATGATGAT
    GATGACGACG ACGATGATGA TGACGAGGAT GAGGATGATG AGGATGACAG CGAAGAAGAC
    AGGCATGTGA GCCGGTCAGA GAAGGAGCAT CGTTCCAGGA GACGAAGACG AAGAGGTGAT
    GATTACGATG ATGATGAGGA CGAGGATGAC GATGATGAGG AGGACAGCGA AGAACAGGAC
    TATGAGGGAG AGGACGATGA AATGGAGGAC TTGGATGATG GAGGAGATGA AGATGATGAG
    GATGGAGAAA ACCAATCAGA CTCTGATCCT GATCCACCAG TTTTGCTCGT CTCAGACTTG
    AACGATGACC TGCTGAACGA TTCCTACCTG ACCGTGACCC TCCATCGGCC GCCGAAGGCC
    AAACGTGACC CTGGCTCAAT TGTCCCTAAA CTAGAGGCGG CGATGTCCCC CCGCACGGGT
    GGCAGTTCTG GCTTCATGCA GCGAAAAACT CTTCCCAAAG CGCGGCCCAA GACCAGCAGC
    TCCACCCCTG CTGGAAACGG CCTCTCTCAG GCTAAAAGCG GCCGTCCCAC GAGGCGAAGC
    TCCAACCAAG ATGGCAGAGA GAGAGACACG GCGTCGCCCT CCTCCATGTC GTCTCGCTCC
    TCTGTTTCTC CACCTCCTCC TCCTCCAGCT GTCACCTCTT CCTCTCCTTC CCTTCTCTCA
    CATCCTTCAT TCCGTGATGC AGGAAACGAG CTCGGCTGTG AAAAGGACAT TTGGGTGTCT
    GTGTTTCGTT ACCTGGATCG GACAGATTTA GCTGTGTGCA TGAGAGTCTG CAAGGCTTGG
    TTTAAATGGT GTTGTGATAA ACGCTTATGG ACACGGATAG ATCTGAGTCG CTGTCGGGCT
    CTCAGTCCTC AGGCTGTGAC GGCTATCATT AAACTACAAC CTGTGACCCT TGACCTCTCC
    TGGACACCTG TGTCAAAGAA ACAGCTGGCC TGGCTCATTC ATCACCTCCC AAGTTTAAAA
    GATCTAATAA TGTCCGGTTG TTCTTCATTA TGTGTGTCTG CATTAAGCTC GCCGAGCTGT
    CCGAGTTTGC GAACTCTTGA CCTGTGTTGG GCTGTGGGCG TCAAAGACTC GCAGATAAAA
    GATCTTATTG TGCAGCCAGG CAGTGAAAGT CGCAGTCGTC TCAGAAGTTT GGTCAGTCTG
    CGCTTGTCTG GTTTGGAATT GAGTGACGCT GTCATTAAGA CGATGGTCCG ACACATGCCT
    TCACTGCGCC AGTTAGATCT GTCCTACTGT CAGGGCCTCA CAGACCAGTC CATCAACCTG
    CTCACTGCTA CTGGATGCAA CACTCGCAAC ACACTCAGAC AGCTCAATCT TTCAGGTTGT
    AATAAGCTGT CAGATGGCTG TCTCTCATAT ATGAAGCGAT TATCCGCTCT GGCTCTGTTG
    GATCTGCGGG GCTGCAAGAA CGTGACCCGA CATGGCTGCG AAAACTTCAT CTCGGACCTG
    TCCTACTGCA CCGTATTCTG CCTGACCGAG GACAAACTCA TTCAGAGAGT ATCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa46193
    Clone ID Related Accession (Same CDS sequence) XM_009293357.2 , XM_009293357.3
    Accession Version XM_009293357.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3882bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product lysine (K)-specific demethylase 2B isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009293357.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGACGATC ACAAACCGCG CTACAGCAAG CGACTGCGGT CTGGCACGCG GAGGCGTTAC 
    CAAGACGATG GCATTTCCGA CGACGAGATC GATGGAAGGA GGACTTTCGA TCTGGAAGAG
    AAGCTACACA GCAGCTTCTT CAGCTCAGAC CGAGTCAAAC GCATGGAGGG CAAAGATTTC
    TCTTATGAGT ACGTCCAGAG GCATGGTCTC CGGGATCCTA TCATCTTTGA GAAACCCGAA
    GGTCTTGGCA TCAAAATGCC AGATCCAGAC TTCACAGTAA ATGATGTGAA GTTGTTTGTA
    GGAAGTCGAC GGATAGTTGA CGTGATGGAC GTGGCTACTC AGAAAGGGAC GGAGATGTCG
    ATGGCCCAGT GGAGGCGCTA CTACGAGACT CCTCCCTCTC AGAGAGAAAA GCTTTATAAT
    GTAATCAGCC TTGAGTTTAG CCACACTAAA CTAGAGACCC TTGTCAAAAG ACCCTCCACG
    GTGGACATGA TTGACTGGGT GGACAACATG TGGCCTCGAC ACTTAAAGGA AAGACAAAGA
    GATTCTACAA ACTCTATCAT GGAGATGCAA TACCCCAAAG TGCAAAAGTA CTGCCTGATG
    AGTGTGCAGG GCTGTTACAC AGATTTCCAC ATAGACTTTG GAGGCACGTC TGTGTGGTAC
    CACATACATA AAGGCTGCAA GGTGTTTTGG TTAATCCCTC CCACGCCTCA AAACCTGGAG
    CTTTATGAGA ACTGGGTGTT GTCAGGGAAA CAAGGCGACA TCTTTCTTGG TGACAGGGCC
    ACTGATTGCC AGAGGATTGA GCTCAAGCAG GGCTACACGT TCATCATTCC ATCAGGCTGG
    ATCCATGCTG TGTACACACC AGTGGACACG CTTGTCTTTG GTGGGAATTT TCTTCATAGT
    TTTAACGTCC CCATGCAATT GGACATCTCC GGCATTGAAG ACAGGACACG GGTGCCTGTG
    AAGTTCCGCT ATCCGTTCTT CTTTGAGATG TGCTGGTATG TGTTGGAGCG TTATCTCTAC
    AGCTTGACCA ACAAATCCCA CCTCATCCCG GAGTTCCAGA AACACTCACT GGGCATTGGC
    TTGAAGCGGG AAAACTCTGA GGTGCTGAAT GGGCATGCAA AAGAGGAAAG AGATGAGATG
    GATGCCCCCT CTCCCCCTAG TGCACCAGGG TTAAAGACTC ACTTGACTGC TTTCGAGCTG
    GAAGGCCTGT GGAGGCTGGT AGGGAAGCTG GAATCGCTGC CCTCAAATAA GAAGTGTGTG
    CCTTCAGGAA TACACAATGC TGCTGCCCTG CTGCTGGACA TACGGGCATT GTTAAAAGAA
    CATGCAAATG ACATCCCTAA GCTCTCTTAC ACTGGAAGAC CCATTGTCAG GTGGCCAAAG
    AGGCCTCCCT GGTACCAGCC CCCTCCTCCG CCTCCACCTG TCATGCGTCC CAAGCTGGCG
    ACCACACCTA TTGTCCCACG GCCTGTCAAA CCAGCCTCCA ACATGTCGGT CCTGCGCCGG
    CGCCGCGTGC GTTGCAAACG CTGCGAGGCC TGTCTGCGCA CAGAATGTGG CGACTGCAAC
    TTCTGCCGAG ACATGAAGAA ATTTGGCGGC CCTGGCAAGC TCAAACAGAC CTGCGTCCTG
    CGGCAATGTC TTGCTCCTGG TCTGCCGCTC TCTGCTGTGT GTGAGCTCTG TGGCGAAGGG
    AATCAGGAGA CCAGTGAAGA GCTGATGGAG TGTTCCAACT GTGCTCAGAT TGCACATCCC
    AGCTGTTTGA AGACGTCTGG TGAGGGAGTG GTGAACAAAG ACCTGCCGAG CTGCTGGGAG
    TGTCCCAAGT GTGTTTTGGG AAAAGACACC GATTCTGAGT CGTCAGGCAG CGGTGATGAC
    ATCACGGCAC AGGATGGGTC AGGGGGGCGA GGGGGCGAGG GCGGGGCGTG GCATGGGGTC
    GGGCGGCCCC CCTCTTCTCT CTCTCACGGC TCGCTCCAGA AACGGGCAGC AGCCCCTGAG
    CAGCGGCTCA GGAAGAGGAT TAAACTGGAT GGTGGTAAAT CACATAAGCG CAAGTCTTCA
    TCTCTTGATC CCCGCGTGGC TAAAATCTAC CGGAGACATG GGATGGAGCA CGACGATGAT
    TCAGGCAGCG ATGATGACGG AGACGGTGGC AGAATGTCTT CAGTGCGCAG CAGCGTTTCC
    TCATCTCGCA GAGGTTATGG AGCTAGCAGG CGAGGCGTTT GGAGAGGCTC CTCACAGCGG
    GGCCCTCGAG GGGGCGGAAG CCTGTCCTCA AGTTCCCTCA AAATGAGGAG AGGACTCGGA
    GCAAGAGGAG AGAGAGGAGG TCGGGTGAGG TTGCGAGGAG GATCGCAGAT GAGGAGACGG
    CGGTATGAAA CTGATGATGA TGATGATGAC GACGATGATG ATGATGATGA CGACGACGAT
    GATGATGACG AGGATGAGGA TGATGAGGAT GACAGCGAAG AAGACAGGCA TGTGAGCCGG
    TCAGAGAAGG AGCATCGTTC CAGGAGACGA AGACGAAGAG GTGATGATTA CGATGATGAT
    GAGGACGAGG ATGACGATGA TGAGGAGGAC AGCGAAGAAC AGGACTATGA GGGAGAGGAC
    GATGAAATGG AGGACTTGGA TGATGGAGGA GATGAAGATG ATGAGGATGG AGAAAACCAA
    TCAGACTCTG ATCCTGATCC ACCAGTTTTG CTCGTCTCAG ACTTGAACGA TGACCTGCTG
    AACGATTCCT ACCTGACCGT GACCCTCCAT CGGCCGCCGA AGGCCAAACG TGACCCTGGC
    TCAATTGTCC CTAAACTAGA GGCGGCGATG TCCCCCCGCA CGGGTGGCAG TTCTGGCTTC
    ATGCAGCGAA AAACTCTTCC CAAAGCGCGG CCCAAGACCA GCAGCTCCAC CCCTGCTGGA
    AACGGCCTCT CTCAGGCTAA AAGCGGCCGT CCCACGAGGC GAAGCTCCAA CCAAGATGGC
    AGAGAGAGAG ACACGGCGTC GCCCTCCTCC ATGTCGTCTC GCTCCTCTGT TTCTCCACCT
    CCTCCTCCTC CAGCTGTCAC CTCTTCCTCT CCTTCCCTTC TCTCACATCC TTCATTCCGT
    GATGCAGGAA ACGAGCTCGG CTGTGAAAAG GACATTTGGG TGTCTGTGTT TCGTTACCTG
    GATCGGACAG ATTTAGCTGT GTGCATGAGA GTCTGCAAGG CTTGGTTTAA ATGGTGTTGT
    GATAAACGCT TATGGACACG GATAGATCTG AGTCGCTGTC GGGCTCTCAG TCCTCAGGCT
    GTGACGGCTA TCATTAAACT ACAACCTGTG ACCCTTGACC TCTCCTGGAC ACCTGTGTCA
    AAGAAACAGC TGGCCTGGCT CATTCATCAC CTCCCAAGTT TAAAAGATCT AATAATGTCC
    GGTTGTTCTT CATTATGTGT GTCTGCATTA AGCTCGCCGA GCTGTCCGAG TTTGCGAACT
    CTTGACCTGT GTTGGGCTGT GGGCGTCAAA GACTCGCAGA TAAAAGATCT TATTGTGCAG
    CCAGGCAGTG AAAGTCGCAG TCGTCTCAGA AGTTTGGTCA GTCTGCGCTT GTCTGGTTTG
    GAATTGAGTG ACGCTGTCAT TAAGACGATG GTCCGACACA TGCCTTCACT GCGCCAGTTA
    GATCTGTCCT ACTGTCAGGG CCTCACAGAC CAGTCCATCA ACCTGCTCAC TGCTACTGGA
    TGCAACACTC GCAACACACT CAGACAGCTC AATCTTTCAG GTTGTAATAA GCTGTCAGAT
    GGCTGTCTCT CATATATGAA GCGATTATCC GCTCTGGCTC TGTTGGATCT GCGGGGCTGC
    AAGAACGTGA CCCGACATGG CTGCGAAAAC TTCATCTCGG ACCTGTCCTA CTGCACCGTA
    TTCTGCCTGA CCGAGGACAA ACTCATTCAG AGAGTATCCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009291632.1
    CDS779..4660
    Translation

    Target ORF information:

    RefSeq Version XM_009293357.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio lysine (K)-specific demethylase 2Aa (kdm2aa), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009293357.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGACGATC ACAAACCGCG CTACAGCAAG CGACTGCGGT CTGGCACGCG GAGGCGTTAC 
    CAAGACGATG GCATTTCCGA CGACGAGATC GATGGAAGGA GGACTTTCGA TCTGGAAGAG
    AAGCTACACA GCAGCTTCTT CAGCTCAGAC CGAGTCAAAC GCATGGAGGG CAAAGATTTC
    TCTTATGAGT ACGTCCAGAG GCATGGTCTC CGGGATCCTA TCATCTTTGA GAAACCCGAA
    GGTCTTGGCA TCAAAATGCC AGATCCAGAC TTCACAGTAA ATGATGTGAA GTTGTTTGTA
    GGAAGTCGAC GGATAGTTGA CGTGATGGAC GTGGCTACTC AGAAAGGGAC GGAGATGTCG
    ATGGCCCAGT GGAGGCGCTA CTACGAGACT CCTCCCTCTC AGAGAGAAAA GCTTTATAAT
    GTAATCAGCC TTGAGTTTAG CCACACTAAA CTAGAGACCC TTGTCAAAAG ACCCTCCACG
    GTGGACATGA TTGACTGGGT GGACAACATG TGGCCTCGAC ACTTAAAGGA AAGACAAAGA
    GATTCTACAA ACTCTATCAT GGAGATGCAA TACCCCAAAG TGCAAAAGTA CTGCCTGATG
    AGTGTGCAGG GCTGTTACAC AGATTTCCAC ATAGACTTTG GAGGCACGTC TGTGTGGTAC
    CACATACATA AAGGCTGCAA GGTGTTTTGG TTAATCCCTC CCACGCCTCA AAACCTGGAG
    CTTTATGAGA ACTGGGTGTT GTCAGGGAAA CAAGGCGACA TCTTTCTTGG TGACAGGGCC
    ACTGATTGCC AGAGGATTGA GCTCAAGCAG GGCTACACGT TCATCATTCC ATCAGGCTGG
    ATCCATGCTG TGTACACACC AGTGGACACG CTTGTCTTTG GTGGGAATTT TCTTCATAGT
    TTTAACGTCC CCATGCAATT GGACATCTCC GGCATTGAAG ACAGGACACG GGTGCCTGTG
    AAGTTCCGCT ATCCGTTCTT CTTTGAGATG TGCTGGTATG TGTTGGAGCG TTATCTCTAC
    AGCTTGACCA ACAAATCCCA CCTCATCCCG GAGTTCCAGA AACACTCACT GGGCATTGGC
    TTGAAGCGGG AAAACTCTGA GGTGCTGAAT GGGCATGCAA AAGAGGAAAG AGATGAGATG
    GATGCCCCCT CTCCCCCTAG TGCACCAGGG TTAAAGACTC ACTTGACTGC TTTCGAGCTG
    GAAGGCCTGT GGAGGCTGGT AGGGAAGCTG GAATCGCTGC CCTCAAATAA GAAGTGTGTG
    CCTTCAGGAA TACACAATGC TGCTGCCCTG CTGCTGGACA TACGGGCATT GTTAAAAGAA
    CATGCAAATG ACATCCCTAA GCTCTCTTAC ACTGGAAGAC CCATTGTCAG GTGGCCAAAG
    AGGCCTCCCT GGTACCAGCC CCCTCCTCCG CCTCCACCTG TCATGCGTCC CAAGCTGGCG
    ACCACACCTA TTGTCCCACG GCCTGTCAAA CCAGCCTCCA ACATGTCGGT CCTGCGCCGG
    CGCCGCGTGC GTTGCAAACG CTGCGAGGCC TGTCTGCGCA CAGAATGTGG CGACTGCAAC
    TTCTGCCGAG ACATGAAGAA ATTTGGCGGC CCTGGCAAGC TCAAACAGAC CTGCGTCCTG
    CGGCAATGTC TTGCTCCTGG TCTGCCGCTC TCTGCTGTGT GTGAGCTCTG TGGCGAAGGG
    AATCAGGAGA CCAGTGAAGA GCTGATGGAG TGTTCCAACT GTGCTCAGAT TGCACATCCC
    AGCTGTTTGA AGACGTCTGG TGAGGGAGTG GTGAACAAAG ACCTGCCGAG CTGCTGGGAG
    TGTCCCAAGT GTGTTTTGGG AAAAGACACC GATTCTGAGT CGTCAGGCAG CGGTGATGAC
    ATCACGGCAC AGGATGGGTC AGGGGGGCGA GGGGGCGAGG GCGGGGCGTG GCATGGGGTC
    GGGCGGCCCC CCTCTTCTCT CTCTCACGGC TCGCTCCAGA AACGGGCAGC AGCCCCTGAG
    CAGCGGCTCA GGAAGAGGAT TAAACTGGAT GGTGGTAAAT CACATAAGCG CAAGTCTTCA
    TCTCTTGATC CCCGCGTGGC TAAAATCTAC CGGAGACATG GGATGGAGCA CGACGATGAT
    TCAGGCAGCG ATGATGACGG AGACGGTGGC AGAATGTCTT CAGTGCGCAG CAGCGTTTCC
    TCATCTCGCA GAGGTTATGG AGCTAGCAGG CGAGGCGTTT GGAGAGGCTC CTCACAGCGG
    GGCCCTCGAG GGGGCGGAAG CCTGTCCTCA AGTTCCCTCA AAATGAGGAG AGGACTCGGA
    GCAAGAGGAG AGAGAGGAGG TCGGGTGAGG TTGCGAGGAG GATCGCAGAT GAGGAGACGG
    CGGTATGAAA CTGATGATGA TGATGATGAC GACGATGATG ATGATGATGA CGACGACGAT
    GATGATGACG AGGATGAGGA TGATGAGGAT GACAGCGAAG AAGACAGGCA TGTGAGCCGG
    TCAGAGAAGG AGCATCGTTC CAGGAGACGA AGACGAAGAG GTGATGATTA CGATGATGAT
    GAGGACGAGG ATGACGATGA TGAGGAGGAC AGCGAAGAAC AGGACTATGA GGGAGAGGAC
    GATGAAATGG AGGACTTGGA TGATGGAGGA GATGAAGATG ATGAGGATGG AGAAAACCAA
    TCAGACTCTG ATCCTGATCC ACCAGTTTTG CTCGTCTCAG ACTTGAACGA TGACCTGCTG
    AACGATTCCT ACCTGACCGT GACCCTCCAT CGGCCGCCGA AGGCCAAACG TGACCCTGGC
    TCAATTGTCC CTAAACTAGA GGCGGCGATG TCCCCCCGCA CGGGTGGCAG TTCTGGCTTC
    ATGCAGCGAA AAACTCTTCC CAAAGCGCGG CCCAAGACCA GCAGCTCCAC CCCTGCTGGA
    AACGGCCTCT CTCAGGCTAA AAGCGGCCGT CCCACGAGGC GAAGCTCCAA CCAAGATGGC
    AGAGAGAGAG ACACGGCGTC GCCCTCCTCC ATGTCGTCTC GCTCCTCTGT TTCTCCACCT
    CCTCCTCCTC CAGCTGTCAC CTCTTCCTCT CCTTCCCTTC TCTCACATCC TTCATTCCGT
    GATGCAGGAA ACGAGCTCGG CTGTGAAAAG GACATTTGGG TGTCTGTGTT TCGTTACCTG
    GATCGGACAG ATTTAGCTGT GTGCATGAGA GTCTGCAAGG CTTGGTTTAA ATGGTGTTGT
    GATAAACGCT TATGGACACG GATAGATCTG AGTCGCTGTC GGGCTCTCAG TCCTCAGGCT
    GTGACGGCTA TCATTAAACT ACAACCTGTG ACCCTTGACC TCTCCTGGAC ACCTGTGTCA
    AAGAAACAGC TGGCCTGGCT CATTCATCAC CTCCCAAGTT TAAAAGATCT AATAATGTCC
    GGTTGTTCTT CATTATGTGT GTCTGCATTA AGCTCGCCGA GCTGTCCGAG TTTGCGAACT
    CTTGACCTGT GTTGGGCTGT GGGCGTCAAA GACTCGCAGA TAAAAGATCT TATTGTGCAG
    CCAGGCAGTG AAAGTCGCAG TCGTCTCAGA AGTTTGGTCA GTCTGCGCTT GTCTGGTTTG
    GAATTGAGTG ACGCTGTCAT TAAGACGATG GTCCGACACA TGCCTTCACT GCGCCAGTTA
    GATCTGTCCT ACTGTCAGGG CCTCACAGAC CAGTCCATCA ACCTGCTCAC TGCTACTGGA
    TGCAACACTC GCAACACACT CAGACAGCTC AATCTTTCAG GTTGTAATAA GCTGTCAGAT
    GGCTGTCTCT CATATATGAA GCGATTATCC GCTCTGGCTC TGTTGGATCT GCGGGGCTGC
    AAGAACGTGA CCCGACATGG CTGCGAAAAC TTCATCTCGG ACCTGTCCTA CTGCACCGTA
    TTCTGCCTGA CCGAGGACAA ACTCATTCAG AGAGTATCCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa46193
    Clone ID Related Accession (Same CDS sequence) XM_009293357.2 , XM_009293357.3
    Accession Version XM_009293357.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3882bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product lysine (K)-specific demethylase 2B isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009293357.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGACGATC ACAAACCGCG CTACAGCAAG CGACTGCGGT CTGGCACGCG GAGGCGTTAC 
    CAAGACGATG GCATTTCCGA CGACGAGATC GATGGAAGGA GGACTTTCGA TCTGGAAGAG
    AAGCTACACA GCAGCTTCTT CAGCTCAGAC CGAGTCAAAC GCATGGAGGG CAAAGATTTC
    TCTTATGAGT ACGTCCAGAG GCATGGTCTC CGGGATCCTA TCATCTTTGA GAAACCCGAA
    GGTCTTGGCA TCAAAATGCC AGATCCAGAC TTCACAGTAA ATGATGTGAA GTTGTTTGTA
    GGAAGTCGAC GGATAGTTGA CGTGATGGAC GTGGCTACTC AGAAAGGGAC GGAGATGTCG
    ATGGCCCAGT GGAGGCGCTA CTACGAGACT CCTCCCTCTC AGAGAGAAAA GCTTTATAAT
    GTAATCAGCC TTGAGTTTAG CCACACTAAA CTAGAGACCC TTGTCAAAAG ACCCTCCACG
    GTGGACATGA TTGACTGGGT GGACAACATG TGGCCTCGAC ACTTAAAGGA AAGACAAAGA
    GATTCTACAA ACTCTATCAT GGAGATGCAA TACCCCAAAG TGCAAAAGTA CTGCCTGATG
    AGTGTGCAGG GCTGTTACAC AGATTTCCAC ATAGACTTTG GAGGCACGTC TGTGTGGTAC
    CACATACATA AAGGCTGCAA GGTGTTTTGG TTAATCCCTC CCACGCCTCA AAACCTGGAG
    CTTTATGAGA ACTGGGTGTT GTCAGGGAAA CAAGGCGACA TCTTTCTTGG TGACAGGGCC
    ACTGATTGCC AGAGGATTGA GCTCAAGCAG GGCTACACGT TCATCATTCC ATCAGGCTGG
    ATCCATGCTG TGTACACACC AGTGGACACG CTTGTCTTTG GTGGGAATTT TCTTCATAGT
    TTTAACGTCC CCATGCAATT GGACATCTCC GGCATTGAAG ACAGGACACG GGTGCCTGTG
    AAGTTCCGCT ATCCGTTCTT CTTTGAGATG TGCTGGTATG TGTTGGAGCG TTATCTCTAC
    AGCTTGACCA ACAAATCCCA CCTCATCCCG GAGTTCCAGA AACACTCACT GGGCATTGGC
    TTGAAGCGGG AAAACTCTGA GGTGCTGAAT GGGCATGCAA AAGAGGAAAG AGATGAGATG
    GATGCCCCCT CTCCCCCTAG TGCACCAGGG TTAAAGACTC ACTTGACTGC TTTCGAGCTG
    GAAGGCCTGT GGAGGCTGGT AGGGAAGCTG GAATCGCTGC CCTCAAATAA GAAGTGTGTG
    CCTTCAGGAA TACACAATGC TGCTGCCCTG CTGCTGGACA TACGGGCATT GTTAAAAGAA
    CATGCAAATG ACATCCCTAA GCTCTCTTAC ACTGGAAGAC CCATTGTCAG GTGGCCAAAG
    AGGCCTCCCT GGTACCAGCC CCCTCCTCCG CCTCCACCTG TCATGCGTCC CAAGCTGGCG
    ACCACACCTA TTGTCCCACG GCCTGTCAAA CCAGCCTCCA ACATGTCGGT CCTGCGCCGG
    CGCCGCGTGC GTTGCAAACG CTGCGAGGCC TGTCTGCGCA CAGAATGTGG CGACTGCAAC
    TTCTGCCGAG ACATGAAGAA ATTTGGCGGC CCTGGCAAGC TCAAACAGAC CTGCGTCCTG
    CGGCAATGTC TTGCTCCTGG TCTGCCGCTC TCTGCTGTGT GTGAGCTCTG TGGCGAAGGG
    AATCAGGAGA CCAGTGAAGA GCTGATGGAG TGTTCCAACT GTGCTCAGAT TGCACATCCC
    AGCTGTTTGA AGACGTCTGG TGAGGGAGTG GTGAACAAAG ACCTGCCGAG CTGCTGGGAG
    TGTCCCAAGT GTGTTTTGGG AAAAGACACC GATTCTGAGT CGTCAGGCAG CGGTGATGAC
    ATCACGGCAC AGGATGGGTC AGGGGGGCGA GGGGGCGAGG GCGGGGCGTG GCATGGGGTC
    GGGCGGCCCC CCTCTTCTCT CTCTCACGGC TCGCTCCAGA AACGGGCAGC AGCCCCTGAG
    CAGCGGCTCA GGAAGAGGAT TAAACTGGAT GGTGGTAAAT CACATAAGCG CAAGTCTTCA
    TCTCTTGATC CCCGCGTGGC TAAAATCTAC CGGAGACATG GGATGGAGCA CGACGATGAT
    TCAGGCAGCG ATGATGACGG AGACGGTGGC AGAATGTCTT CAGTGCGCAG CAGCGTTTCC
    TCATCTCGCA GAGGTTATGG AGCTAGCAGG CGAGGCGTTT GGAGAGGCTC CTCACAGCGG
    GGCCCTCGAG GGGGCGGAAG CCTGTCCTCA AGTTCCCTCA AAATGAGGAG AGGACTCGGA
    GCAAGAGGAG AGAGAGGAGG TCGGGTGAGG TTGCGAGGAG GATCGCAGAT GAGGAGACGG
    CGGTATGAAA CTGATGATGA TGATGATGAC GACGATGATG ATGATGATGA CGACGACGAT
    GATGATGACG AGGATGAGGA TGATGAGGAT GACAGCGAAG AAGACAGGCA TGTGAGCCGG
    TCAGAGAAGG AGCATCGTTC CAGGAGACGA AGACGAAGAG GTGATGATTA CGATGATGAT
    GAGGACGAGG ATGACGATGA TGAGGAGGAC AGCGAAGAAC AGGACTATGA GGGAGAGGAC
    GATGAAATGG AGGACTTGGA TGATGGAGGA GATGAAGATG ATGAGGATGG AGAAAACCAA
    TCAGACTCTG ATCCTGATCC ACCAGTTTTG CTCGTCTCAG ACTTGAACGA TGACCTGCTG
    AACGATTCCT ACCTGACCGT GACCCTCCAT CGGCCGCCGA AGGCCAAACG TGACCCTGGC
    TCAATTGTCC CTAAACTAGA GGCGGCGATG TCCCCCCGCA CGGGTGGCAG TTCTGGCTTC
    ATGCAGCGAA AAACTCTTCC CAAAGCGCGG CCCAAGACCA GCAGCTCCAC CCCTGCTGGA
    AACGGCCTCT CTCAGGCTAA AAGCGGCCGT CCCACGAGGC GAAGCTCCAA CCAAGATGGC
    AGAGAGAGAG ACACGGCGTC GCCCTCCTCC ATGTCGTCTC GCTCCTCTGT TTCTCCACCT
    CCTCCTCCTC CAGCTGTCAC CTCTTCCTCT CCTTCCCTTC TCTCACATCC TTCATTCCGT
    GATGCAGGAA ACGAGCTCGG CTGTGAAAAG GACATTTGGG TGTCTGTGTT TCGTTACCTG
    GATCGGACAG ATTTAGCTGT GTGCATGAGA GTCTGCAAGG CTTGGTTTAA ATGGTGTTGT
    GATAAACGCT TATGGACACG GATAGATCTG AGTCGCTGTC GGGCTCTCAG TCCTCAGGCT
    GTGACGGCTA TCATTAAACT ACAACCTGTG ACCCTTGACC TCTCCTGGAC ACCTGTGTCA
    AAGAAACAGC TGGCCTGGCT CATTCATCAC CTCCCAAGTT TAAAAGATCT AATAATGTCC
    GGTTGTTCTT CATTATGTGT GTCTGCATTA AGCTCGCCGA GCTGTCCGAG TTTGCGAACT
    CTTGACCTGT GTTGGGCTGT GGGCGTCAAA GACTCGCAGA TAAAAGATCT TATTGTGCAG
    CCAGGCAGTG AAAGTCGCAG TCGTCTCAGA AGTTTGGTCA GTCTGCGCTT GTCTGGTTTG
    GAATTGAGTG ACGCTGTCAT TAAGACGATG GTCCGACACA TGCCTTCACT GCGCCAGTTA
    GATCTGTCCT ACTGTCAGGG CCTCACAGAC CAGTCCATCA ACCTGCTCAC TGCTACTGGA
    TGCAACACTC GCAACACACT CAGACAGCTC AATCTTTCAG GTTGTAATAA GCTGTCAGAT
    GGCTGTCTCT CATATATGAA GCGATTATCC GCTCTGGCTC TGTTGGATCT GCGGGGCTGC
    AAGAACGTGA CCCGACATGG CTGCGAAAAC TTCATCTCGG ACCTGTCCTA CTGCACCGTA
    TTCTGCCTGA CCGAGGACAA ACTCATTCAG AGAGTATCCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009291632.1
    CDS799..4680
    Translation

    Target ORF information:

    RefSeq Version XM_009293357.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio lysine (K)-specific demethylase 2Aa (kdm2aa), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009293357.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGACGATC ACAAACCGCG CTACAGCAAG CGACTGCGGT CTGGCACGCG GAGGCGTTAC 
    CAAGACGATG GCATTTCCGA CGACGAGATC GATGGAAGGA GGACTTTCGA TCTGGAAGAG
    AAGCTACACA GCAGCTTCTT CAGCTCAGAC CGAGTCAAAC GCATGGAGGG CAAAGATTTC
    TCTTATGAGT ACGTCCAGAG GCATGGTCTC CGGGATCCTA TCATCTTTGA GAAACCCGAA
    GGTCTTGGCA TCAAAATGCC AGATCCAGAC TTCACAGTAA ATGATGTGAA GTTGTTTGTA
    GGAAGTCGAC GGATAGTTGA CGTGATGGAC GTGGCTACTC AGAAAGGGAC GGAGATGTCG
    ATGGCCCAGT GGAGGCGCTA CTACGAGACT CCTCCCTCTC AGAGAGAAAA GCTTTATAAT
    GTAATCAGCC TTGAGTTTAG CCACACTAAA CTAGAGACCC TTGTCAAAAG ACCCTCCACG
    GTGGACATGA TTGACTGGGT GGACAACATG TGGCCTCGAC ACTTAAAGGA AAGACAAAGA
    GATTCTACAA ACTCTATCAT GGAGATGCAA TACCCCAAAG TGCAAAAGTA CTGCCTGATG
    AGTGTGCAGG GCTGTTACAC AGATTTCCAC ATAGACTTTG GAGGCACGTC TGTGTGGTAC
    CACATACATA AAGGCTGCAA GGTGTTTTGG TTAATCCCTC CCACGCCTCA AAACCTGGAG
    CTTTATGAGA ACTGGGTGTT GTCAGGGAAA CAAGGCGACA TCTTTCTTGG TGACAGGGCC
    ACTGATTGCC AGAGGATTGA GCTCAAGCAG GGCTACACGT TCATCATTCC ATCAGGCTGG
    ATCCATGCTG TGTACACACC AGTGGACACG CTTGTCTTTG GTGGGAATTT TCTTCATAGT
    TTTAACGTCC CCATGCAATT GGACATCTCC GGCATTGAAG ACAGGACACG GGTGCCTGTG
    AAGTTCCGCT ATCCGTTCTT CTTTGAGATG TGCTGGTATG TGTTGGAGCG TTATCTCTAC
    AGCTTGACCA ACAAATCCCA CCTCATCCCG GAGTTCCAGA AACACTCACT GGGCATTGGC
    TTGAAGCGGG AAAACTCTGA GGTGCTGAAT GGGCATGCAA AAGAGGAAAG AGATGAGATG
    GATGCCCCCT CTCCCCCTAG TGCACCAGGG TTAAAGACTC ACTTGACTGC TTTCGAGCTG
    GAAGGCCTGT GGAGGCTGGT AGGGAAGCTG GAATCGCTGC CCTCAAATAA GAAGTGTGTG
    CCTTCAGGAA TACACAATGC TGCTGCCCTG CTGCTGGACA TACGGGCATT GTTAAAAGAA
    CATGCAAATG ACATCCCTAA GCTCTCTTAC ACTGGAAGAC CCATTGTCAG GTGGCCAAAG
    AGGCCTCCCT GGTACCAGCC CCCTCCTCCG CCTCCACCTG TCATGCGTCC CAAGCTGGCG
    ACCACACCTA TTGTCCCACG GCCTGTCAAA CCAGCCTCCA ACATGTCGGT CCTGCGCCGG
    CGCCGCGTGC GTTGCAAACG CTGCGAGGCC TGTCTGCGCA CAGAATGTGG CGACTGCAAC
    TTCTGCCGAG ACATGAAGAA ATTTGGCGGC CCTGGCAAGC TCAAACAGAC CTGCGTCCTG
    CGGCAATGTC TTGCTCCTGG TCTGCCGCTC TCTGCTGTGT GTGAGCTCTG TGGCGAAGGG
    AATCAGGAGA CCAGTGAAGA GCTGATGGAG TGTTCCAACT GTGCTCAGAT TGCACATCCC
    AGCTGTTTGA AGACGTCTGG TGAGGGAGTG GTGAACAAAG ACCTGCCGAG CTGCTGGGAG
    TGTCCCAAGT GTGTTTTGGG AAAAGACACC GATTCTGAGT CGTCAGGCAG CGGTGATGAC
    ATCACGGCAC AGGATGGGTC AGGGGGGCGA GGGGGCGAGG GCGGGGCGTG GCATGGGGTC
    GGGCGGCCCC CCTCTTCTCT CTCTCACGGC TCGCTCCAGA AACGGGCAGC AGCCCCTGAG
    CAGCGGCTCA GGAAGAGGAT TAAACTGGAT GGTGGTAAAT CACATAAGCG CAAGTCTTCA
    TCTCTTGATC CCCGCGTGGC TAAAATCTAC CGGAGACATG GGATGGAGCA CGACGATGAT
    TCAGGCAGCG ATGATGACGG AGACGGTGGC AGAATGTCTT CAGTGCGCAG CAGCGTTTCC
    TCATCTCGCA GAGGTTATGG AGCTAGCAGG CGAGGCGTTT GGAGAGGCTC CTCACAGCGG
    GGCCCTCGAG GGGGCGGAAG CCTGTCCTCA AGTTCCCTCA AAATGAGGAG AGGACTCGGA
    GCAAGAGGAG AGAGAGGAGG TCGGGTGAGG TTGCGAGGAG GATCGCAGAT GAGGAGACGG
    CGGTATGAAA CTGATGATGA TGATGATGAC GACGATGATG ATGATGATGA CGACGACGAT
    GATGATGACG AGGATGAGGA TGATGAGGAT GACAGCGAAG AAGACAGGCA TGTGAGCCGG
    TCAGAGAAGG AGCATCGTTC CAGGAGACGA AGACGAAGAG GTGATGATTA CGATGATGAT
    GAGGACGAGG ATGACGATGA TGAGGAGGAC AGCGAAGAAC AGGACTATGA GGGAGAGGAC
    GATGAAATGG AGGACTTGGA TGATGGAGGA GATGAAGATG ATGAGGATGG AGAAAACCAA
    TCAGACTCTG ATCCTGATCC ACCAGTTTTG CTCGTCTCAG ACTTGAACGA TGACCTGCTG
    AACGATTCCT ACCTGACCGT GACCCTCCAT CGGCCGCCGA AGGCCAAACG TGACCCTGGC
    TCAATTGTCC CTAAACTAGA GGCGGCGATG TCCCCCCGCA CGGGTGGCAG TTCTGGCTTC
    ATGCAGCGAA AAACTCTTCC CAAAGCGCGG CCCAAGACCA GCAGCTCCAC CCCTGCTGGA
    AACGGCCTCT CTCAGGCTAA AAGCGGCCGT CCCACGAGGC GAAGCTCCAA CCAAGATGGC
    AGAGAGAGAG ACACGGCGTC GCCCTCCTCC ATGTCGTCTC GCTCCTCTGT TTCTCCACCT
    CCTCCTCCTC CAGCTGTCAC CTCTTCCTCT CCTTCCCTTC TCTCACATCC TTCATTCCGT
    GATGCAGGAA ACGAGCTCGG CTGTGAAAAG GACATTTGGG TGTCTGTGTT TCGTTACCTG
    GATCGGACAG ATTTAGCTGT GTGCATGAGA GTCTGCAAGG CTTGGTTTAA ATGGTGTTGT
    GATAAACGCT TATGGACACG GATAGATCTG AGTCGCTGTC GGGCTCTCAG TCCTCAGGCT
    GTGACGGCTA TCATTAAACT ACAACCTGTG ACCCTTGACC TCTCCTGGAC ACCTGTGTCA
    AAGAAACAGC TGGCCTGGCT CATTCATCAC CTCCCAAGTT TAAAAGATCT AATAATGTCC
    GGTTGTTCTT CATTATGTGT GTCTGCATTA AGCTCGCCGA GCTGTCCGAG TTTGCGAACT
    CTTGACCTGT GTTGGGCTGT GGGCGTCAAA GACTCGCAGA TAAAAGATCT TATTGTGCAG
    CCAGGCAGTG AAAGTCGCAG TCGTCTCAGA AGTTTGGTCA GTCTGCGCTT GTCTGGTTTG
    GAATTGAGTG ACGCTGTCAT TAAGACGATG GTCCGACACA TGCCTTCACT GCGCCAGTTA
    GATCTGTCCT ACTGTCAGGG CCTCACAGAC CAGTCCATCA ACCTGCTCAC TGCTACTGGA
    TGCAACACTC GCAACACACT CAGACAGCTC AATCTTTCAG GTTGTAATAA GCTGTCAGAT
    GGCTGTCTCT CATATATGAA GCGATTATCC GCTCTGGCTC TGTTGGATCT GCGGGGCTGC
    AAGAACGTGA CCCGACATGG CTGCGAAAAC TTCATCTCGG ACCTGTCCTA CTGCACCGTA
    TTCTGCCTGA CCGAGGACAA ACTCATTCAG AGAGTATCCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa46194
    Clone ID Related Accession (Same CDS sequence) XM_009293362.2 , XM_009293362.3
    Accession Version XM_009293362.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3798bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product lysine (K)-specific demethylase 2B isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009293362.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGAGAACG GACAGGAGGT GCTTACGCTT GTTGACGGGG GGATGTTGCT GATGAGACGG 
    AGAGGCAGGA AGAGGAAAAG CCAGCAGGGG GATGAAGATG TGACACTGAA GCGGTCTGGC
    ACGCGGAGGC GTTACCAAGA CGATGGCATT TCCGACGACG AGATCGATGG AAGGAGGACT
    TTCGATCTGG AAGAGAAGCT ACACAGCAGC TTCTTCAGCT CAGACCGAGT CAAACGCATG
    GAGGGCAAAG ATTTCTCTTA TGAGTACGTC CAGAGGCATG GTCTCCGGGA TCCTATCATC
    TTTGAGAAAC CCGAAGGTCT TGGCATCAAA ATGCCAGATC CAGACTTCAC AGTAAATGAT
    GTGAAGTTGT TTGTAGGAAG TCGACGGATA GTTGACGTGA TGGACGTGGC TACTCAGAAA
    GGGACGGAGA TGTCGATGGC CCAGTGGAGG CGCTACTACG AGACTCCTCC CTCTCAGAGA
    GAAAAGCTTT ATAATGTAAT CAGCCTTGAG TTTAGCCACA CTAAACTAGA GACCCTTGTC
    AAAAGACCCT CCACGGTGGA CATGATTGAC TGGGTGGACA ACATGTGGCC TCGACACTTA
    AAGGAAAGAC AAAGAGATTC TACAAACTCT ATCATGGAGA TGCAATACCC CAAAGTGCAA
    AAGTACTGCC TGATGAGTGT GCAGGGCTGT TACACAGATT TCCACATAGA CTTTGGAGGC
    ACGTCTGTGT GGTACCACAT ACATAAAGGC TGCAAGGTGT TTTGGTTAAT CCCTCCCACG
    CCTCAAAACC TGGAGCTTTA TGAGAACTGG GTGTTGTCAG GGAAACAAGG CGACATCTTT
    CTTGGTGACA GGGCCACTGA TTGCCAGAGG ATTGAGCTCA AGCAGGGCTA CACGTTCATC
    ATTCCATCAG GCTGGATCCA TGCTGTGTAC ACACCAGTGG ACACGCTTGT CTTTGGTGGG
    AATTTTCTTC ATAGTTTTAA CGTCCCCATG CAATTGGACA TCTCCGGCAT TGAAGACAGG
    ACACGGGTGC CTGTGAAGTT CCGCTATCCG TTCTTCTTTG AGATGTGCTG GTATGTGTTG
    GAGCGTTATC TCTACAGCTT GACCAACAAA TCCCACCTCA TCCCGGAGTT CCAGAAACAC
    TCACTGGGCA TTGGCTTGAA GCGGGAAAAC TCTGAGGTGC TGAATGGGCA TGCAAAAGAG
    GAAAGAGATG AGATGGATGC CCCCTCTCCC CCTAGTGCAC CAGGGTTAAA GACTCACTTG
    ACTGCTTTCG AGCTGGAAGG CCTGTGGAGG CTGGTAGGGA AGCTGGAATC GCTGCCCTCA
    AATAAGAAGT GTGTGCCTTC AGGAATACAC AATGCTGCTG CCCTGCTGCT GGACATACGG
    GCATTGTTAA AAGAACATGC AAATGACATC CCTAAGCTCT CTTACACTGG AAGACCCATT
    GTCAGGTGGC CAAAGAGGCC TCCCTGGTAC CAGCCCCCTC CTCCGCCTCC ACCTGTCATG
    CGTCCCAAGC TGGCGACCAC ACCTATTGTC CCACGGCCTG TCAAACCAGC CTCCAACATG
    TCGGTCCTGC GCCGGCGCCG CGTGCGTTGC AAACGCTGCG AGGCCTGTCT GCGCACAGAA
    TGTGGCGACT GCAACTTCTG CCGAGACATG AAGAAATTTG GCGGCCCTGG CAAGCTCAAA
    CAGACCTGCG TCCTGCGGCA ATGTCTTGCT CCTGGTCTGC CGCTCTCTGC TGTGTGTGAG
    CTCTGTGGCG AAGGGAATCA GGAGACCAGT GAAGAGCTGA TGGAGTGTTC CAACTGTGCT
    CAGATTGCAC ATCCCAGCTG TTTGAAGACG TCTGGTGAGG GAGTGGTGAA CAAAGACCTG
    CCGAGCTGCT GGGAGTGTCC CAAGTGTGTT TTGGGAAAAG ACACCGATTC TGAGATTAAA
    CTGGATGGTG GTAAATCACA TAAGCGCAAG TCTTCATCTC TTGATCCCCG CGTGGCTAAA
    ATCTACCGGA GACATGGGAT GGAGCACGAC GATGATTCAG GCAGCGATGA TGACGGAGAC
    GGTGGCAGAA TGTCTTCAGT GCGCAGCAGC GTTTCCTCAT CTCGCAGAGG TTATGGAGCT
    AGCAGGCGAG GCGTTTGGAG AGGCTCCTCA CAGCGGGGCC CTCGAGGGGG CGGAAGCCTG
    TCCTCAAGTT CCCTCAAAAT GAGGAGAGGA CTCGGAGCAA GAGGAGAGAG AGGAGGTCGG
    GTGAGGTTGC GAGGAGGATC GCAGATGAGG AGACGGCGGT ATGAAACTGA TGATGATGAT
    GATGACGACG ATGATGATGA TGATGACGAC GACGATGATG ATGACGAGGA TGAGGATGAT
    GAGGATGACA GCGAAGAAGA CAGGCATGTG AGCCGGTCAG AGAAGGAGCA TCGTTCCAGG
    AGACGAAGAC GAAGAGGTGA TGATTACGAT GATGATGAGG ACGAGGATGA CGATGATGAG
    GAGGACAGCG AAGAACAGGA CTATGAGGGA GAGGACGATG AAATGGAGGA CTTGGATGAT
    GGAGGAGATG AAGATGATGA GGATGGAGAA AACCAATCAG ACTCTGATCC TGATCCACCA
    GTTTTGCTCG TCTCAGACTT GAACGATGAC CTGCTGAACG ATTCCTACCT GACCGTGACC
    CTCCATCGGC CGCCGAAGGC CAAACGTGAC CCTGGCTCAA TTGTCCCTAA ACTAGAGGCG
    GCGATGTCCC CCCGCACGGG TGGCAGTTCT GGCTTCATGC AGCGAAAAAC TCTTCCCAAA
    GCGCGGCCCA AGACCAGCAG CTCCACCCCT GCTGGAAACG GCCTCTCTCA GGCTAAAAGC
    GGCCGTCCCA CGAGGCGAAG CTCCAACCAA GATGGCAGAG AGAGAGACAC GGCGTCGCCC
    TCCTCCATGT CGTCTCGCTC CTCTGTTTCT CCACCTCCTC CTCCTCCAGC TGTCACCTCT
    TCCTCTCCTT CCCTTCTCTC ACATCCTTCA TTCCGTGATG CAGGAAACGA GCTCGGCTGT
    GAAAAGGACA TTTGGGTGTC TGTGTTTCGT TACCTGGATC GGACAGATTT AGCTGTGTGC
    ATGAGAGTCT GCAAGGCTTG GTTTAAATGG TGTTGTGATA AACGCTTATG GACACGGATA
    GATCTGAGTC GCTGTCGGGC TCTCAGTCCT CAGGCTGTGA CGGCTATCAT TAAACTACAA
    CCTGTGACCC TTGACCTCTC CTGGACACCT GTGTCAAAGA AACAGCTGGC CTGGCTCATT
    CATCACCTCC CAAGTTTAAA AGATCTAATA ATGTCCGGTT GTTCTTCATT ATGTGTGTCT
    GCATTAAGCT CGCCGAGCTG TCCGAGTTTG CGAACTCTTG ACCTGTGTTG GGCTGTGGGC
    GTCAAAGACT CGCAGATAAA AGATCTTATT GTGCAGCCAG GCAGTGAAAG TCGCAGTCGT
    CTCAGAAGTT TGGTCAGTCT GCGCTTGTCT GGTTTGGAAT TGAGTGACGC TGTCATTAAG
    ACGATGGTCC GACACATGCC TTCACTGCGC CAGTTAGATC TGTCCTACTG TCAGGGCCTC
    ACAGACCAGT CCATCAACCT GCTCACTGCT ACTGGATGCA ACACTCGCAA CACACTCAGA
    CAGCTCAATC TTTCAGGTTG TAATAAGCTG TCAGATGGCT GTCTCTCATA TATGAAGCGA
    TTATCCGCTC TGGCTCTGTT GGATCTGCGG GGCTGCAAGA ACGTGACCCG ACATGGCTGC
    GAAAACTTCA TCTCGGACCT GTCCTACTGC ACCGTATTCT GCCTGACCGA GGACAAACTC
    ATTCAGAGAG TATCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009291637.1
    CDS236..4033
    Translation

    Target ORF information:

    RefSeq Version XM_009293362.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio lysine (K)-specific demethylase 2Aa (kdm2aa), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009293362.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGAGAACG GACAGGAGGT GCTTACGCTT GTTGACGGGG GGATGTTGCT GATGAGACGG 
    AGAGGCAGGA AGAGGAAAAG CCAGCAGGGG GATGAAGATG TGACACTGAA GCGGTCTGGC
    ACGCGGAGGC GTTACCAAGA CGATGGCATT TCCGACGACG AGATCGATGG AAGGAGGACT
    TTCGATCTGG AAGAGAAGCT ACACAGCAGC TTCTTCAGCT CAGACCGAGT CAAACGCATG
    GAGGGCAAAG ATTTCTCTTA TGAGTACGTC CAGAGGCATG GTCTCCGGGA TCCTATCATC
    TTTGAGAAAC CCGAAGGTCT TGGCATCAAA ATGCCAGATC CAGACTTCAC AGTAAATGAT
    GTGAAGTTGT TTGTAGGAAG TCGACGGATA GTTGACGTGA TGGACGTGGC TACTCAGAAA
    GGGACGGAGA TGTCGATGGC CCAGTGGAGG CGCTACTACG AGACTCCTCC CTCTCAGAGA
    GAAAAGCTTT ATAATGTAAT CAGCCTTGAG TTTAGCCACA CTAAACTAGA GACCCTTGTC
    AAAAGACCCT CCACGGTGGA CATGATTGAC TGGGTGGACA ACATGTGGCC TCGACACTTA
    AAGGAAAGAC AAAGAGATTC TACAAACTCT ATCATGGAGA TGCAATACCC CAAAGTGCAA
    AAGTACTGCC TGATGAGTGT GCAGGGCTGT TACACAGATT TCCACATAGA CTTTGGAGGC
    ACGTCTGTGT GGTACCACAT ACATAAAGGC TGCAAGGTGT TTTGGTTAAT CCCTCCCACG
    CCTCAAAACC TGGAGCTTTA TGAGAACTGG GTGTTGTCAG GGAAACAAGG CGACATCTTT
    CTTGGTGACA GGGCCACTGA TTGCCAGAGG ATTGAGCTCA AGCAGGGCTA CACGTTCATC
    ATTCCATCAG GCTGGATCCA TGCTGTGTAC ACACCAGTGG ACACGCTTGT CTTTGGTGGG
    AATTTTCTTC ATAGTTTTAA CGTCCCCATG CAATTGGACA TCTCCGGCAT TGAAGACAGG
    ACACGGGTGC CTGTGAAGTT CCGCTATCCG TTCTTCTTTG AGATGTGCTG GTATGTGTTG
    GAGCGTTATC TCTACAGCTT GACCAACAAA TCCCACCTCA TCCCGGAGTT CCAGAAACAC
    TCACTGGGCA TTGGCTTGAA GCGGGAAAAC TCTGAGGTGC TGAATGGGCA TGCAAAAGAG
    GAAAGAGATG AGATGGATGC CCCCTCTCCC CCTAGTGCAC CAGGGTTAAA GACTCACTTG
    ACTGCTTTCG AGCTGGAAGG CCTGTGGAGG CTGGTAGGGA AGCTGGAATC GCTGCCCTCA
    AATAAGAAGT GTGTGCCTTC AGGAATACAC AATGCTGCTG CCCTGCTGCT GGACATACGG
    GCATTGTTAA AAGAACATGC AAATGACATC CCTAAGCTCT CTTACACTGG AAGACCCATT
    GTCAGGTGGC CAAAGAGGCC TCCCTGGTAC CAGCCCCCTC CTCCGCCTCC ACCTGTCATG
    CGTCCCAAGC TGGCGACCAC ACCTATTGTC CCACGGCCTG TCAAACCAGC CTCCAACATG
    TCGGTCCTGC GCCGGCGCCG CGTGCGTTGC AAACGCTGCG AGGCCTGTCT GCGCACAGAA
    TGTGGCGACT GCAACTTCTG CCGAGACATG AAGAAATTTG GCGGCCCTGG CAAGCTCAAA
    CAGACCTGCG TCCTGCGGCA ATGTCTTGCT CCTGGTCTGC CGCTCTCTGC TGTGTGTGAG
    CTCTGTGGCG AAGGGAATCA GGAGACCAGT GAAGAGCTGA TGGAGTGTTC CAACTGTGCT
    CAGATTGCAC ATCCCAGCTG TTTGAAGACG TCTGGTGAGG GAGTGGTGAA CAAAGACCTG
    CCGAGCTGCT GGGAGTGTCC CAAGTGTGTT TTGGGAAAAG ACACCGATTC TGAGATTAAA
    CTGGATGGTG GTAAATCACA TAAGCGCAAG TCTTCATCTC TTGATCCCCG CGTGGCTAAA
    ATCTACCGGA GACATGGGAT GGAGCACGAC GATGATTCAG GCAGCGATGA TGACGGAGAC
    GGTGGCAGAA TGTCTTCAGT GCGCAGCAGC GTTTCCTCAT CTCGCAGAGG TTATGGAGCT
    AGCAGGCGAG GCGTTTGGAG AGGCTCCTCA CAGCGGGGCC CTCGAGGGGG CGGAAGCCTG
    TCCTCAAGTT CCCTCAAAAT GAGGAGAGGA CTCGGAGCAA GAGGAGAGAG AGGAGGTCGG
    GTGAGGTTGC GAGGAGGATC GCAGATGAGG AGACGGCGGT ATGAAACTGA TGATGATGAT
    GATGACGACG ATGATGATGA TGATGACGAC GACGATGATG ATGACGAGGA TGAGGATGAT
    GAGGATGACA GCGAAGAAGA CAGGCATGTG AGCCGGTCAG AGAAGGAGCA TCGTTCCAGG
    AGACGAAGAC GAAGAGGTGA TGATTACGAT GATGATGAGG ACGAGGATGA CGATGATGAG
    GAGGACAGCG AAGAACAGGA CTATGAGGGA GAGGACGATG AAATGGAGGA CTTGGATGAT
    GGAGGAGATG AAGATGATGA GGATGGAGAA AACCAATCAG ACTCTGATCC TGATCCACCA
    GTTTTGCTCG TCTCAGACTT GAACGATGAC CTGCTGAACG ATTCCTACCT GACCGTGACC
    CTCCATCGGC CGCCGAAGGC CAAACGTGAC CCTGGCTCAA TTGTCCCTAA ACTAGAGGCG
    GCGATGTCCC CCCGCACGGG TGGCAGTTCT GGCTTCATGC AGCGAAAAAC TCTTCCCAAA
    GCGCGGCCCA AGACCAGCAG CTCCACCCCT GCTGGAAACG GCCTCTCTCA GGCTAAAAGC
    GGCCGTCCCA CGAGGCGAAG CTCCAACCAA GATGGCAGAG AGAGAGACAC GGCGTCGCCC
    TCCTCCATGT CGTCTCGCTC CTCTGTTTCT CCACCTCCTC CTCCTCCAGC TGTCACCTCT
    TCCTCTCCTT CCCTTCTCTC ACATCCTTCA TTCCGTGATG CAGGAAACGA GCTCGGCTGT
    GAAAAGGACA TTTGGGTGTC TGTGTTTCGT TACCTGGATC GGACAGATTT AGCTGTGTGC
    ATGAGAGTCT GCAAGGCTTG GTTTAAATGG TGTTGTGATA AACGCTTATG GACACGGATA
    GATCTGAGTC GCTGTCGGGC TCTCAGTCCT CAGGCTGTGA CGGCTATCAT TAAACTACAA
    CCTGTGACCC TTGACCTCTC CTGGACACCT GTGTCAAAGA AACAGCTGGC CTGGCTCATT
    CATCACCTCC CAAGTTTAAA AGATCTAATA ATGTCCGGTT GTTCTTCATT ATGTGTGTCT
    GCATTAAGCT CGCCGAGCTG TCCGAGTTTG CGAACTCTTG ACCTGTGTTG GGCTGTGGGC
    GTCAAAGACT CGCAGATAAA AGATCTTATT GTGCAGCCAG GCAGTGAAAG TCGCAGTCGT
    CTCAGAAGTT TGGTCAGTCT GCGCTTGTCT GGTTTGGAAT TGAGTGACGC TGTCATTAAG
    ACGATGGTCC GACACATGCC TTCACTGCGC CAGTTAGATC TGTCCTACTG TCAGGGCCTC
    ACAGACCAGT CCATCAACCT GCTCACTGCT ACTGGATGCA ACACTCGCAA CACACTCAGA
    CAGCTCAATC TTTCAGGTTG TAATAAGCTG TCAGATGGCT GTCTCTCATA TATGAAGCGA
    TTATCCGCTC TGGCTCTGTT GGATCTGCGG GGCTGCAAGA ACGTGACCCG ACATGGCTGC
    GAAAACTTCA TCTCGGACCT GTCCTACTGC ACCGTATTCT GCCTGACCGA GGACAAACTC
    ATTCAGAGAG TATCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa46194
    Clone ID Related Accession (Same CDS sequence) XM_009293362.2 , XM_009293362.3
    Accession Version XM_009293362.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3798bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product lysine (K)-specific demethylase 2B isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009293362.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGAGAACG GACAGGAGGT GCTTACGCTT GTTGACGGGG GGATGTTGCT GATGAGACGG 
    AGAGGCAGGA AGAGGAAAAG CCAGCAGGGG GATGAAGATG TGACACTGAA GCGGTCTGGC
    ACGCGGAGGC GTTACCAAGA CGATGGCATT TCCGACGACG AGATCGATGG AAGGAGGACT
    TTCGATCTGG AAGAGAAGCT ACACAGCAGC TTCTTCAGCT CAGACCGAGT CAAACGCATG
    GAGGGCAAAG ATTTCTCTTA TGAGTACGTC CAGAGGCATG GTCTCCGGGA TCCTATCATC
    TTTGAGAAAC CCGAAGGTCT TGGCATCAAA ATGCCAGATC CAGACTTCAC AGTAAATGAT
    GTGAAGTTGT TTGTAGGAAG TCGACGGATA GTTGACGTGA TGGACGTGGC TACTCAGAAA
    GGGACGGAGA TGTCGATGGC CCAGTGGAGG CGCTACTACG AGACTCCTCC CTCTCAGAGA
    GAAAAGCTTT ATAATGTAAT CAGCCTTGAG TTTAGCCACA CTAAACTAGA GACCCTTGTC
    AAAAGACCCT CCACGGTGGA CATGATTGAC TGGGTGGACA ACATGTGGCC TCGACACTTA
    AAGGAAAGAC AAAGAGATTC TACAAACTCT ATCATGGAGA TGCAATACCC CAAAGTGCAA
    AAGTACTGCC TGATGAGTGT GCAGGGCTGT TACACAGATT TCCACATAGA CTTTGGAGGC
    ACGTCTGTGT GGTACCACAT ACATAAAGGC TGCAAGGTGT TTTGGTTAAT CCCTCCCACG
    CCTCAAAACC TGGAGCTTTA TGAGAACTGG GTGTTGTCAG GGAAACAAGG CGACATCTTT
    CTTGGTGACA GGGCCACTGA TTGCCAGAGG ATTGAGCTCA AGCAGGGCTA CACGTTCATC
    ATTCCATCAG GCTGGATCCA TGCTGTGTAC ACACCAGTGG ACACGCTTGT CTTTGGTGGG
    AATTTTCTTC ATAGTTTTAA CGTCCCCATG CAATTGGACA TCTCCGGCAT TGAAGACAGG
    ACACGGGTGC CTGTGAAGTT CCGCTATCCG TTCTTCTTTG AGATGTGCTG GTATGTGTTG
    GAGCGTTATC TCTACAGCTT GACCAACAAA TCCCACCTCA TCCCGGAGTT CCAGAAACAC
    TCACTGGGCA TTGGCTTGAA GCGGGAAAAC TCTGAGGTGC TGAATGGGCA TGCAAAAGAG
    GAAAGAGATG AGATGGATGC CCCCTCTCCC CCTAGTGCAC CAGGGTTAAA GACTCACTTG
    ACTGCTTTCG AGCTGGAAGG CCTGTGGAGG CTGGTAGGGA AGCTGGAATC GCTGCCCTCA
    AATAAGAAGT GTGTGCCTTC AGGAATACAC AATGCTGCTG CCCTGCTGCT GGACATACGG
    GCATTGTTAA AAGAACATGC AAATGACATC CCTAAGCTCT CTTACACTGG AAGACCCATT
    GTCAGGTGGC CAAAGAGGCC TCCCTGGTAC CAGCCCCCTC CTCCGCCTCC ACCTGTCATG
    CGTCCCAAGC TGGCGACCAC ACCTATTGTC CCACGGCCTG TCAAACCAGC CTCCAACATG
    TCGGTCCTGC GCCGGCGCCG CGTGCGTTGC AAACGCTGCG AGGCCTGTCT GCGCACAGAA
    TGTGGCGACT GCAACTTCTG CCGAGACATG AAGAAATTTG GCGGCCCTGG CAAGCTCAAA
    CAGACCTGCG TCCTGCGGCA ATGTCTTGCT CCTGGTCTGC CGCTCTCTGC TGTGTGTGAG
    CTCTGTGGCG AAGGGAATCA GGAGACCAGT GAAGAGCTGA TGGAGTGTTC CAACTGTGCT
    CAGATTGCAC ATCCCAGCTG TTTGAAGACG TCTGGTGAGG GAGTGGTGAA CAAAGACCTG
    CCGAGCTGCT GGGAGTGTCC CAAGTGTGTT TTGGGAAAAG ACACCGATTC TGAGATTAAA
    CTGGATGGTG GTAAATCACA TAAGCGCAAG TCTTCATCTC TTGATCCCCG CGTGGCTAAA
    ATCTACCGGA GACATGGGAT GGAGCACGAC GATGATTCAG GCAGCGATGA TGACGGAGAC
    GGTGGCAGAA TGTCTTCAGT GCGCAGCAGC GTTTCCTCAT CTCGCAGAGG TTATGGAGCT
    AGCAGGCGAG GCGTTTGGAG AGGCTCCTCA CAGCGGGGCC CTCGAGGGGG CGGAAGCCTG
    TCCTCAAGTT CCCTCAAAAT GAGGAGAGGA CTCGGAGCAA GAGGAGAGAG AGGAGGTCGG
    GTGAGGTTGC GAGGAGGATC GCAGATGAGG AGACGGCGGT ATGAAACTGA TGATGATGAT
    GATGACGACG ATGATGATGA TGATGACGAC GACGATGATG ATGACGAGGA TGAGGATGAT
    GAGGATGACA GCGAAGAAGA CAGGCATGTG AGCCGGTCAG AGAAGGAGCA TCGTTCCAGG
    AGACGAAGAC GAAGAGGTGA TGATTACGAT GATGATGAGG ACGAGGATGA CGATGATGAG
    GAGGACAGCG AAGAACAGGA CTATGAGGGA GAGGACGATG AAATGGAGGA CTTGGATGAT
    GGAGGAGATG AAGATGATGA GGATGGAGAA AACCAATCAG ACTCTGATCC TGATCCACCA
    GTTTTGCTCG TCTCAGACTT GAACGATGAC CTGCTGAACG ATTCCTACCT GACCGTGACC
    CTCCATCGGC CGCCGAAGGC CAAACGTGAC CCTGGCTCAA TTGTCCCTAA ACTAGAGGCG
    GCGATGTCCC CCCGCACGGG TGGCAGTTCT GGCTTCATGC AGCGAAAAAC TCTTCCCAAA
    GCGCGGCCCA AGACCAGCAG CTCCACCCCT GCTGGAAACG GCCTCTCTCA GGCTAAAAGC
    GGCCGTCCCA CGAGGCGAAG CTCCAACCAA GATGGCAGAG AGAGAGACAC GGCGTCGCCC
    TCCTCCATGT CGTCTCGCTC CTCTGTTTCT CCACCTCCTC CTCCTCCAGC TGTCACCTCT
    TCCTCTCCTT CCCTTCTCTC ACATCCTTCA TTCCGTGATG CAGGAAACGA GCTCGGCTGT
    GAAAAGGACA TTTGGGTGTC TGTGTTTCGT TACCTGGATC GGACAGATTT AGCTGTGTGC
    ATGAGAGTCT GCAAGGCTTG GTTTAAATGG TGTTGTGATA AACGCTTATG GACACGGATA
    GATCTGAGTC GCTGTCGGGC TCTCAGTCCT CAGGCTGTGA CGGCTATCAT TAAACTACAA
    CCTGTGACCC TTGACCTCTC CTGGACACCT GTGTCAAAGA AACAGCTGGC CTGGCTCATT
    CATCACCTCC CAAGTTTAAA AGATCTAATA ATGTCCGGTT GTTCTTCATT ATGTGTGTCT
    GCATTAAGCT CGCCGAGCTG TCCGAGTTTG CGAACTCTTG ACCTGTGTTG GGCTGTGGGC
    GTCAAAGACT CGCAGATAAA AGATCTTATT GTGCAGCCAG GCAGTGAAAG TCGCAGTCGT
    CTCAGAAGTT TGGTCAGTCT GCGCTTGTCT GGTTTGGAAT TGAGTGACGC TGTCATTAAG
    ACGATGGTCC GACACATGCC TTCACTGCGC CAGTTAGATC TGTCCTACTG TCAGGGCCTC
    ACAGACCAGT CCATCAACCT GCTCACTGCT ACTGGATGCA ACACTCGCAA CACACTCAGA
    CAGCTCAATC TTTCAGGTTG TAATAAGCTG TCAGATGGCT GTCTCTCATA TATGAAGCGA
    TTATCCGCTC TGGCTCTGTT GGATCTGCGG GGCTGCAAGA ACGTGACCCG ACATGGCTGC
    GAAAACTTCA TCTCGGACCT GTCCTACTGC ACCGTATTCT GCCTGACCGA GGACAAACTC
    ATTCAGAGAG TATCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009291637.1
    CDS95..3892
    Translation

    Target ORF information:

    RefSeq Version XM_009293362.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio lysine (K)-specific demethylase 2Aa (kdm2aa), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009293362.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGAGAACG GACAGGAGGT GCTTACGCTT GTTGACGGGG GGATGTTGCT GATGAGACGG 
    AGAGGCAGGA AGAGGAAAAG CCAGCAGGGG GATGAAGATG TGACACTGAA GCGGTCTGGC
    ACGCGGAGGC GTTACCAAGA CGATGGCATT TCCGACGACG AGATCGATGG AAGGAGGACT
    TTCGATCTGG AAGAGAAGCT ACACAGCAGC TTCTTCAGCT CAGACCGAGT CAAACGCATG
    GAGGGCAAAG ATTTCTCTTA TGAGTACGTC CAGAGGCATG GTCTCCGGGA TCCTATCATC
    TTTGAGAAAC CCGAAGGTCT TGGCATCAAA ATGCCAGATC CAGACTTCAC AGTAAATGAT
    GTGAAGTTGT TTGTAGGAAG TCGACGGATA GTTGACGTGA TGGACGTGGC TACTCAGAAA
    GGGACGGAGA TGTCGATGGC CCAGTGGAGG CGCTACTACG AGACTCCTCC CTCTCAGAGA
    GAAAAGCTTT ATAATGTAAT CAGCCTTGAG TTTAGCCACA CTAAACTAGA GACCCTTGTC
    AAAAGACCCT CCACGGTGGA CATGATTGAC TGGGTGGACA ACATGTGGCC TCGACACTTA
    AAGGAAAGAC AAAGAGATTC TACAAACTCT ATCATGGAGA TGCAATACCC CAAAGTGCAA
    AAGTACTGCC TGATGAGTGT GCAGGGCTGT TACACAGATT TCCACATAGA CTTTGGAGGC
    ACGTCTGTGT GGTACCACAT ACATAAAGGC TGCAAGGTGT TTTGGTTAAT CCCTCCCACG
    CCTCAAAACC TGGAGCTTTA TGAGAACTGG GTGTTGTCAG GGAAACAAGG CGACATCTTT
    CTTGGTGACA GGGCCACTGA TTGCCAGAGG ATTGAGCTCA AGCAGGGCTA CACGTTCATC
    ATTCCATCAG GCTGGATCCA TGCTGTGTAC ACACCAGTGG ACACGCTTGT CTTTGGTGGG
    AATTTTCTTC ATAGTTTTAA CGTCCCCATG CAATTGGACA TCTCCGGCAT TGAAGACAGG
    ACACGGGTGC CTGTGAAGTT CCGCTATCCG TTCTTCTTTG AGATGTGCTG GTATGTGTTG
    GAGCGTTATC TCTACAGCTT GACCAACAAA TCCCACCTCA TCCCGGAGTT CCAGAAACAC
    TCACTGGGCA TTGGCTTGAA GCGGGAAAAC TCTGAGGTGC TGAATGGGCA TGCAAAAGAG
    GAAAGAGATG AGATGGATGC CCCCTCTCCC CCTAGTGCAC CAGGGTTAAA GACTCACTTG
    ACTGCTTTCG AGCTGGAAGG CCTGTGGAGG CTGGTAGGGA AGCTGGAATC GCTGCCCTCA
    AATAAGAAGT GTGTGCCTTC AGGAATACAC AATGCTGCTG CCCTGCTGCT GGACATACGG
    GCATTGTTAA AAGAACATGC AAATGACATC CCTAAGCTCT CTTACACTGG AAGACCCATT
    GTCAGGTGGC CAAAGAGGCC TCCCTGGTAC CAGCCCCCTC CTCCGCCTCC ACCTGTCATG
    CGTCCCAAGC TGGCGACCAC ACCTATTGTC CCACGGCCTG TCAAACCAGC CTCCAACATG
    TCGGTCCTGC GCCGGCGCCG CGTGCGTTGC AAACGCTGCG AGGCCTGTCT GCGCACAGAA
    TGTGGCGACT GCAACTTCTG CCGAGACATG AAGAAATTTG GCGGCCCTGG CAAGCTCAAA
    CAGACCTGCG TCCTGCGGCA ATGTCTTGCT CCTGGTCTGC CGCTCTCTGC TGTGTGTGAG
    CTCTGTGGCG AAGGGAATCA GGAGACCAGT GAAGAGCTGA TGGAGTGTTC CAACTGTGCT
    CAGATTGCAC ATCCCAGCTG TTTGAAGACG TCTGGTGAGG GAGTGGTGAA CAAAGACCTG
    CCGAGCTGCT GGGAGTGTCC CAAGTGTGTT TTGGGAAAAG ACACCGATTC TGAGATTAAA
    CTGGATGGTG GTAAATCACA TAAGCGCAAG TCTTCATCTC TTGATCCCCG CGTGGCTAAA
    ATCTACCGGA GACATGGGAT GGAGCACGAC GATGATTCAG GCAGCGATGA TGACGGAGAC
    GGTGGCAGAA TGTCTTCAGT GCGCAGCAGC GTTTCCTCAT CTCGCAGAGG TTATGGAGCT
    AGCAGGCGAG GCGTTTGGAG AGGCTCCTCA CAGCGGGGCC CTCGAGGGGG CGGAAGCCTG
    TCCTCAAGTT CCCTCAAAAT GAGGAGAGGA CTCGGAGCAA GAGGAGAGAG AGGAGGTCGG
    GTGAGGTTGC GAGGAGGATC GCAGATGAGG AGACGGCGGT ATGAAACTGA TGATGATGAT
    GATGACGACG ATGATGATGA TGATGACGAC GACGATGATG ATGACGAGGA TGAGGATGAT
    GAGGATGACA GCGAAGAAGA CAGGCATGTG AGCCGGTCAG AGAAGGAGCA TCGTTCCAGG
    AGACGAAGAC GAAGAGGTGA TGATTACGAT GATGATGAGG ACGAGGATGA CGATGATGAG
    GAGGACAGCG AAGAACAGGA CTATGAGGGA GAGGACGATG AAATGGAGGA CTTGGATGAT
    GGAGGAGATG AAGATGATGA GGATGGAGAA AACCAATCAG ACTCTGATCC TGATCCACCA
    GTTTTGCTCG TCTCAGACTT GAACGATGAC CTGCTGAACG ATTCCTACCT GACCGTGACC
    CTCCATCGGC CGCCGAAGGC CAAACGTGAC CCTGGCTCAA TTGTCCCTAA ACTAGAGGCG
    GCGATGTCCC CCCGCACGGG TGGCAGTTCT GGCTTCATGC AGCGAAAAAC TCTTCCCAAA
    GCGCGGCCCA AGACCAGCAG CTCCACCCCT GCTGGAAACG GCCTCTCTCA GGCTAAAAGC
    GGCCGTCCCA CGAGGCGAAG CTCCAACCAA GATGGCAGAG AGAGAGACAC GGCGTCGCCC
    TCCTCCATGT CGTCTCGCTC CTCTGTTTCT CCACCTCCTC CTCCTCCAGC TGTCACCTCT
    TCCTCTCCTT CCCTTCTCTC ACATCCTTCA TTCCGTGATG CAGGAAACGA GCTCGGCTGT
    GAAAAGGACA TTTGGGTGTC TGTGTTTCGT TACCTGGATC GGACAGATTT AGCTGTGTGC
    ATGAGAGTCT GCAAGGCTTG GTTTAAATGG TGTTGTGATA AACGCTTATG GACACGGATA
    GATCTGAGTC GCTGTCGGGC TCTCAGTCCT CAGGCTGTGA CGGCTATCAT TAAACTACAA
    CCTGTGACCC TTGACCTCTC CTGGACACCT GTGTCAAAGA AACAGCTGGC CTGGCTCATT
    CATCACCTCC CAAGTTTAAA AGATCTAATA ATGTCCGGTT GTTCTTCATT ATGTGTGTCT
    GCATTAAGCT CGCCGAGCTG TCCGAGTTTG CGAACTCTTG ACCTGTGTTG GGCTGTGGGC
    GTCAAAGACT CGCAGATAAA AGATCTTATT GTGCAGCCAG GCAGTGAAAG TCGCAGTCGT
    CTCAGAAGTT TGGTCAGTCT GCGCTTGTCT GGTTTGGAAT TGAGTGACGC TGTCATTAAG
    ACGATGGTCC GACACATGCC TTCACTGCGC CAGTTAGATC TGTCCTACTG TCAGGGCCTC
    ACAGACCAGT CCATCAACCT GCTCACTGCT ACTGGATGCA ACACTCGCAA CACACTCAGA
    CAGCTCAATC TTTCAGGTTG TAATAAGCTG TCAGATGGCT GTCTCTCATA TATGAAGCGA
    TTATCCGCTC TGGCTCTGTT GGATCTGCGG GGCTGCAAGA ACGTGACCCG ACATGGCTGC
    GAAAACTTCA TCTCGGACCT GTCCTACTGC ACCGTATTCT GCCTGACCGA GGACAAACTC
    ATTCAGAGAG TATCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa46195
    Clone ID Related Accession (Same CDS sequence) XM_009293365.3 , XM_009293365.2
    Accession Version XM_009293365.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3720bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product lysine (K)-specific demethylase 2B isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009293365.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGAGGGCA AAGATTTCTC TTATGAGTAC GTCCAGAGGC ATGGTCTCCG GGATCCTATC 
    ATCTTTGAGA AACCCGAAGG TCTTGGCATC AAAATGCCAG ATCCAGACTT CACAGTAAAT
    GATGTGAAGT TGTTTGTAGG AAGTCGACGG ATAGTTGACG TGATGGACGT GGCTACTCAG
    AAAGGGACGG AGATGTCGAT GGCCCAGTGG AGGCGCTACT ACGAGACTCC TCCCTCTCAG
    AGAGAAAAGC TTTATAATGT AATCAGCCTT GAGTTTAGCC ACACTAAACT AGAGACCCTT
    GTCAAAAGAC CCTCCACGGT GGACATGATT GACTGGGTGG ACAACATGTG GCCTCGACAC
    TTAAAGGAAA GACAAAGAGA TTCTACAAAC TCTATCATGG AGATGCAATA CCCCAAAGTG
    CAAAAGTACT GCCTGATGAG TGTGCAGGGC TGTTACACAG ATTTCCACAT AGACTTTGGA
    GGCACGTCTG TGTGGTACCA CATACATAAA GGCTGCAAGG TGTTTTGGTT AATCCCTCCC
    ACGCCTCAAA ACCTGGAGCT TTATGAGAAC TGGGTGTTGT CAGGGAAACA AGGCGACATC
    TTTCTTGGTG ACAGGGCCAC TGATTGCCAG AGGATTGAGC TCAAGCAGGG CTACACGTTC
    ATCATTCCAT CAGGCTGGAT CCATGCTGTG TACACACCAG TGGACACGCT TGTCTTTGGT
    GGGAATTTTC TTCATAGTTT TAACGTCCCC ATGCAATTGG ACATCTCCGG CATTGAAGAC
    AGGACACGGG TGCCTGTGAA GTTCCGCTAT CCGTTCTTCT TTGAGATGTG CTGGTATGTG
    TTGGAGCGTT ATCTCTACAG CTTGACCAAC AAATCCCACC TCATCCCGGA GTTCCAGAAA
    CACTCACTGG GCATTGGCTT GAAGCGGGAA AACTCTGAGG TGCTGAATGG GCATGCAAAA
    GAGGAAAGAG ATGAGATGGA TGCCCCCTCT CCCCCTAGTG CACCAGGGTT AAAGACTCAC
    TTGACTGCTT TCGAGCTGGA AGGCCTGTGG AGGCTGGTAG GGAAGCTGGA ATCGCTGCCC
    TCAAATAAGA AGTGTGTGCC TTCAGGAATA CACAATGCTG CTGCCCTGCT GCTGGACATA
    CGGGCATTGT TAAAAGAACA TGCAAATGAC ATCCCTAAGC TCTCTTACAC TGGAAGACCC
    ATTGTCAGGT GGCCAAAGAG GCCTCCCTGG TACCAGCCCC CTCCTCCGCC TCCACCTGTC
    ATGCGTCCCA AGCTGGCGAC CACACCTATT GTCCCACGGC CTGTCAAACC AGCCTCCAAC
    ATGTCGGTCC TGCGCCGGCG CCGCGTGCGT TGCAAACGCT GCGAGGCCTG TCTGCGCACA
    GAATGTGGCG ACTGCAACTT CTGCCGAGAC ATGAAGAAAT TTGGCGGCCC TGGCAAGCTC
    AAACAGACCT GCGTCCTGCG GCAATGTCTT GCTCCTGGTC TGCCGCTCTC TGCTGTGTGT
    GAGCTCTGTG GCGAAGGGAA TCAGGAGACC AGTGAAGAGC TGATGGAGTG TTCCAACTGT
    GCTCAGATTG CACATCCCAG CTGTTTGAAG ACGTCTGGTG AGGGAGTGGT GAACAAAGAC
    CTGCCGAGCT GCTGGGAGTG TCCCAAGTGT GTTTTGGGAA AAGACACCGA TTCTGAGTCG
    TCAGGCAGCG GTGATGACAT CACGGCACAG GATGGGTCAG GGGGGCGAGG GGGCGAGGGC
    GGGGCGTGGC ATGGGGTCGG GCGGCCCCCC TCTTCTCTCT CTCACGGCTC GCTCCAGAAA
    CGGGCAGCAG CCCCTGAGCA GCGGCTCAGG AAGAGGATTA AACTGGATGG TGGTAAATCA
    CATAAGCGCA AGTCTTCATC TCTTGATCCC CGCGTGGCTA AAATCTACCG GAGACATGGG
    ATGGAGCACG ACGATGATTC AGGCAGCGAT GATGACGGAG ACGGTGGCAG AATGTCTTCA
    GTGCGCAGCA GCGTTTCCTC ATCTCGCAGA GGTTATGGAG CTAGCAGGCG AGGCGTTTGG
    AGAGGCTCCT CACAGCGGGG CCCTCGAGGG GGCGGAAGCC TGTCCTCAAG TTCCCTCAAA
    ATGAGGAGAG GACTCGGAGC AAGAGGAGAG AGAGGAGGTC GGGTGAGGTT GCGAGGAGGA
    TCGCAGATGA GGAGACGGCG GTATGAAACT GATGATGATG ATGATGACGA CGATGATGAT
    GATGATGACG ACGACGATGA TGATGACGAG GATGAGGATG ATGAGGATGA CAGCGAAGAA
    GACAGGCATG TGAGCCGGTC AGAGAAGGAG CATCGTTCCA GGAGACGAAG ACGAAGAGGT
    GATGATTACG ATGATGATGA GGACGAGGAT GACGATGATG AGGAGGACAG CGAAGAACAG
    GACTATGAGG GAGAGGACGA TGAAATGGAG GACTTGGATG ATGGAGGAGA TGAAGATGAT
    GAGGATGGAG AAAACCAATC AGACTCTGAT CCTGATCCAC CAGTTTTGCT CGTCTCAGAC
    TTGAACGATG ACCTGCTGAA CGATTCCTAC CTGACCGTGA CCCTCCATCG GCCGCCGAAG
    GCCAAACGTG ACCCTGGCTC AATTGTCCCT AAACTAGAGG CGGCGATGTC CCCCCGCACG
    GGTGGCAGTT CTGGCTTCAT GCAGCGAAAA ACTCTTCCCA AAGCGCGGCC CAAGACCAGC
    AGCTCCACCC CTGCTGGAAA CGGCCTCTCT CAGGCTAAAA GCGGCCGTCC CACGAGGCGA
    AGCTCCAACC AAGATGGCAG AGAGAGAGAC ACGGCGTCGC CCTCCTCCAT GTCGTCTCGC
    TCCTCTGTTT CTCCACCTCC TCCTCCTCCA GCTGTCACCT CTTCCTCTCC TTCCCTTCTC
    TCACATCCTT CATTCCGTGA TGCAGGAAAC GAGCTCGGCT GTGAAAAGGA CATTTGGGTG
    TCTGTGTTTC GTTACCTGGA TCGGACAGAT TTAGCTGTGT GCATGAGAGT CTGCAAGGCT
    TGGTTTAAAT GGTGTTGTGA TAAACGCTTA TGGACACGGA TAGATCTGAG TCGCTGTCGG
    GCTCTCAGTC CTCAGGCTGT GACGGCTATC ATTAAACTAC AACCTGTGAC CCTTGACCTC
    TCCTGGACAC CTGTGTCAAA GAAACAGCTG GCCTGGCTCA TTCATCACCT CCCAAGTTTA
    AAAGATCTAA TAATGTCCGG TTGTTCTTCA TTATGTGTGT CTGCATTAAG CTCGCCGAGC
    TGTCCGAGTT TGCGAACTCT TGACCTGTGT TGGGCTGTGG GCGTCAAAGA CTCGCAGATA
    AAAGATCTTA TTGTGCAGCC AGGCAGTGAA AGTCGCAGTC GTCTCAGAAG TTTGGTCAGT
    CTGCGCTTGT CTGGTTTGGA ATTGAGTGAC GCTGTCATTA AGACGATGGT CCGACACATG
    CCTTCACTGC GCCAGTTAGA TCTGTCCTAC TGTCAGGGCC TCACAGACCA GTCCATCAAC
    CTGCTCACTG CTACTGGATG CAACACTCGC AACACACTCA GACAGCTCAA TCTTTCAGGT
    TGTAATAAGC TGTCAGATGG CTGTCTCTCA TATATGAAGC GATTATCCGC TCTGGCTCTG
    TTGGATCTGC GGGGCTGCAA GAACGTGACC CGACATGGCT GCGAAAACTT CATCTCGGAC
    CTGTCCTACT GCACCGTATT CTGCCTGACC GAGGACAAAC TCATTCAGAG AGTATCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009291640.1
    CDS262..3981
    Translation

    Target ORF information:

    RefSeq Version XM_009293365.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio lysine (K)-specific demethylase 2Aa (kdm2aa), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009293365.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGAGGGCA AAGATTTCTC TTATGAGTAC GTCCAGAGGC ATGGTCTCCG GGATCCTATC 
    ATCTTTGAGA AACCCGAAGG TCTTGGCATC AAAATGCCAG ATCCAGACTT CACAGTAAAT
    GATGTGAAGT TGTTTGTAGG AAGTCGACGG ATAGTTGACG TGATGGACGT GGCTACTCAG
    AAAGGGACGG AGATGTCGAT GGCCCAGTGG AGGCGCTACT ACGAGACTCC TCCCTCTCAG
    AGAGAAAAGC TTTATAATGT AATCAGCCTT GAGTTTAGCC ACACTAAACT AGAGACCCTT
    GTCAAAAGAC CCTCCACGGT GGACATGATT GACTGGGTGG ACAACATGTG GCCTCGACAC
    TTAAAGGAAA GACAAAGAGA TTCTACAAAC TCTATCATGG AGATGCAATA CCCCAAAGTG
    CAAAAGTACT GCCTGATGAG TGTGCAGGGC TGTTACACAG ATTTCCACAT AGACTTTGGA
    GGCACGTCTG TGTGGTACCA CATACATAAA GGCTGCAAGG TGTTTTGGTT AATCCCTCCC
    ACGCCTCAAA ACCTGGAGCT TTATGAGAAC TGGGTGTTGT CAGGGAAACA AGGCGACATC
    TTTCTTGGTG ACAGGGCCAC TGATTGCCAG AGGATTGAGC TCAAGCAGGG CTACACGTTC
    ATCATTCCAT CAGGCTGGAT CCATGCTGTG TACACACCAG TGGACACGCT TGTCTTTGGT
    GGGAATTTTC TTCATAGTTT TAACGTCCCC ATGCAATTGG ACATCTCCGG CATTGAAGAC
    AGGACACGGG TGCCTGTGAA GTTCCGCTAT CCGTTCTTCT TTGAGATGTG CTGGTATGTG
    TTGGAGCGTT ATCTCTACAG CTTGACCAAC AAATCCCACC TCATCCCGGA GTTCCAGAAA
    CACTCACTGG GCATTGGCTT GAAGCGGGAA AACTCTGAGG TGCTGAATGG GCATGCAAAA
    GAGGAAAGAG ATGAGATGGA TGCCCCCTCT CCCCCTAGTG CACCAGGGTT AAAGACTCAC
    TTGACTGCTT TCGAGCTGGA AGGCCTGTGG AGGCTGGTAG GGAAGCTGGA ATCGCTGCCC
    TCAAATAAGA AGTGTGTGCC TTCAGGAATA CACAATGCTG CTGCCCTGCT GCTGGACATA
    CGGGCATTGT TAAAAGAACA TGCAAATGAC ATCCCTAAGC TCTCTTACAC TGGAAGACCC
    ATTGTCAGGT GGCCAAAGAG GCCTCCCTGG TACCAGCCCC CTCCTCCGCC TCCACCTGTC
    ATGCGTCCCA AGCTGGCGAC CACACCTATT GTCCCACGGC CTGTCAAACC AGCCTCCAAC
    ATGTCGGTCC TGCGCCGGCG CCGCGTGCGT TGCAAACGCT GCGAGGCCTG TCTGCGCACA
    GAATGTGGCG ACTGCAACTT CTGCCGAGAC ATGAAGAAAT TTGGCGGCCC TGGCAAGCTC
    AAACAGACCT GCGTCCTGCG GCAATGTCTT GCTCCTGGTC TGCCGCTCTC TGCTGTGTGT
    GAGCTCTGTG GCGAAGGGAA TCAGGAGACC AGTGAAGAGC TGATGGAGTG TTCCAACTGT
    GCTCAGATTG CACATCCCAG CTGTTTGAAG ACGTCTGGTG AGGGAGTGGT GAACAAAGAC
    CTGCCGAGCT GCTGGGAGTG TCCCAAGTGT GTTTTGGGAA AAGACACCGA TTCTGAGTCG
    TCAGGCAGCG GTGATGACAT CACGGCACAG GATGGGTCAG GGGGGCGAGG GGGCGAGGGC
    GGGGCGTGGC ATGGGGTCGG GCGGCCCCCC TCTTCTCTCT CTCACGGCTC GCTCCAGAAA
    CGGGCAGCAG CCCCTGAGCA GCGGCTCAGG AAGAGGATTA AACTGGATGG TGGTAAATCA
    CATAAGCGCA AGTCTTCATC TCTTGATCCC CGCGTGGCTA AAATCTACCG GAGACATGGG
    ATGGAGCACG ACGATGATTC AGGCAGCGAT GATGACGGAG ACGGTGGCAG AATGTCTTCA
    GTGCGCAGCA GCGTTTCCTC ATCTCGCAGA GGTTATGGAG CTAGCAGGCG AGGCGTTTGG
    AGAGGCTCCT CACAGCGGGG CCCTCGAGGG GGCGGAAGCC TGTCCTCAAG TTCCCTCAAA
    ATGAGGAGAG GACTCGGAGC AAGAGGAGAG AGAGGAGGTC GGGTGAGGTT GCGAGGAGGA
    TCGCAGATGA GGAGACGGCG GTATGAAACT GATGATGATG ATGATGACGA CGATGATGAT
    GATGATGACG ACGACGATGA TGATGACGAG GATGAGGATG ATGAGGATGA CAGCGAAGAA
    GACAGGCATG TGAGCCGGTC AGAGAAGGAG CATCGTTCCA GGAGACGAAG ACGAAGAGGT
    GATGATTACG ATGATGATGA GGACGAGGAT GACGATGATG AGGAGGACAG CGAAGAACAG
    GACTATGAGG GAGAGGACGA TGAAATGGAG GACTTGGATG ATGGAGGAGA TGAAGATGAT
    GAGGATGGAG AAAACCAATC AGACTCTGAT CCTGATCCAC CAGTTTTGCT CGTCTCAGAC
    TTGAACGATG ACCTGCTGAA CGATTCCTAC CTGACCGTGA CCCTCCATCG GCCGCCGAAG
    GCCAAACGTG ACCCTGGCTC AATTGTCCCT AAACTAGAGG CGGCGATGTC CCCCCGCACG
    GGTGGCAGTT CTGGCTTCAT GCAGCGAAAA ACTCTTCCCA AAGCGCGGCC CAAGACCAGC
    AGCTCCACCC CTGCTGGAAA CGGCCTCTCT CAGGCTAAAA GCGGCCGTCC CACGAGGCGA
    AGCTCCAACC AAGATGGCAG AGAGAGAGAC ACGGCGTCGC CCTCCTCCAT GTCGTCTCGC
    TCCTCTGTTT CTCCACCTCC TCCTCCTCCA GCTGTCACCT CTTCCTCTCC TTCCCTTCTC
    TCACATCCTT CATTCCGTGA TGCAGGAAAC GAGCTCGGCT GTGAAAAGGA CATTTGGGTG
    TCTGTGTTTC GTTACCTGGA TCGGACAGAT TTAGCTGTGT GCATGAGAGT CTGCAAGGCT
    TGGTTTAAAT GGTGTTGTGA TAAACGCTTA TGGACACGGA TAGATCTGAG TCGCTGTCGG
    GCTCTCAGTC CTCAGGCTGT GACGGCTATC ATTAAACTAC AACCTGTGAC CCTTGACCTC
    TCCTGGACAC CTGTGTCAAA GAAACAGCTG GCCTGGCTCA TTCATCACCT CCCAAGTTTA
    AAAGATCTAA TAATGTCCGG TTGTTCTTCA TTATGTGTGT CTGCATTAAG CTCGCCGAGC
    TGTCCGAGTT TGCGAACTCT TGACCTGTGT TGGGCTGTGG GCGTCAAAGA CTCGCAGATA
    AAAGATCTTA TTGTGCAGCC AGGCAGTGAA AGTCGCAGTC GTCTCAGAAG TTTGGTCAGT
    CTGCGCTTGT CTGGTTTGGA ATTGAGTGAC GCTGTCATTA AGACGATGGT CCGACACATG
    CCTTCACTGC GCCAGTTAGA TCTGTCCTAC TGTCAGGGCC TCACAGACCA GTCCATCAAC
    CTGCTCACTG CTACTGGATG CAACACTCGC AACACACTCA GACAGCTCAA TCTTTCAGGT
    TGTAATAAGC TGTCAGATGG CTGTCTCTCA TATATGAAGC GATTATCCGC TCTGGCTCTG
    TTGGATCTGC GGGGCTGCAA GAACGTGACC CGACATGGCT GCGAAAACTT CATCTCGGAC
    CTGTCCTACT GCACCGTATT CTGCCTGACC GAGGACAAAC TCATTCAGAG AGTATCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa46195
    Clone ID Related Accession (Same CDS sequence) XM_009293365.3 , XM_009293365.2
    Accession Version XM_009293365.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3720bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product lysine (K)-specific demethylase 2B isoform X4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009293365.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGAGGGCA AAGATTTCTC TTATGAGTAC GTCCAGAGGC ATGGTCTCCG GGATCCTATC 
    ATCTTTGAGA AACCCGAAGG TCTTGGCATC AAAATGCCAG ATCCAGACTT CACAGTAAAT
    GATGTGAAGT TGTTTGTAGG AAGTCGACGG ATAGTTGACG TGATGGACGT GGCTACTCAG
    AAAGGGACGG AGATGTCGAT GGCCCAGTGG AGGCGCTACT ACGAGACTCC TCCCTCTCAG
    AGAGAAAAGC TTTATAATGT AATCAGCCTT GAGTTTAGCC ACACTAAACT AGAGACCCTT
    GTCAAAAGAC CCTCCACGGT GGACATGATT GACTGGGTGG ACAACATGTG GCCTCGACAC
    TTAAAGGAAA GACAAAGAGA TTCTACAAAC TCTATCATGG AGATGCAATA CCCCAAAGTG
    CAAAAGTACT GCCTGATGAG TGTGCAGGGC TGTTACACAG ATTTCCACAT AGACTTTGGA
    GGCACGTCTG TGTGGTACCA CATACATAAA GGCTGCAAGG TGTTTTGGTT AATCCCTCCC
    ACGCCTCAAA ACCTGGAGCT TTATGAGAAC TGGGTGTTGT CAGGGAAACA AGGCGACATC
    TTTCTTGGTG ACAGGGCCAC TGATTGCCAG AGGATTGAGC TCAAGCAGGG CTACACGTTC
    ATCATTCCAT CAGGCTGGAT CCATGCTGTG TACACACCAG TGGACACGCT TGTCTTTGGT
    GGGAATTTTC TTCATAGTTT TAACGTCCCC ATGCAATTGG ACATCTCCGG CATTGAAGAC
    AGGACACGGG TGCCTGTGAA GTTCCGCTAT CCGTTCTTCT TTGAGATGTG CTGGTATGTG
    TTGGAGCGTT ATCTCTACAG CTTGACCAAC AAATCCCACC TCATCCCGGA GTTCCAGAAA
    CACTCACTGG GCATTGGCTT GAAGCGGGAA AACTCTGAGG TGCTGAATGG GCATGCAAAA
    GAGGAAAGAG ATGAGATGGA TGCCCCCTCT CCCCCTAGTG CACCAGGGTT AAAGACTCAC
    TTGACTGCTT TCGAGCTGGA AGGCCTGTGG AGGCTGGTAG GGAAGCTGGA ATCGCTGCCC
    TCAAATAAGA AGTGTGTGCC TTCAGGAATA CACAATGCTG CTGCCCTGCT GCTGGACATA
    CGGGCATTGT TAAAAGAACA TGCAAATGAC ATCCCTAAGC TCTCTTACAC TGGAAGACCC
    ATTGTCAGGT GGCCAAAGAG GCCTCCCTGG TACCAGCCCC CTCCTCCGCC TCCACCTGTC
    ATGCGTCCCA AGCTGGCGAC CACACCTATT GTCCCACGGC CTGTCAAACC AGCCTCCAAC
    ATGTCGGTCC TGCGCCGGCG CCGCGTGCGT TGCAAACGCT GCGAGGCCTG TCTGCGCACA
    GAATGTGGCG ACTGCAACTT CTGCCGAGAC ATGAAGAAAT TTGGCGGCCC TGGCAAGCTC
    AAACAGACCT GCGTCCTGCG GCAATGTCTT GCTCCTGGTC TGCCGCTCTC TGCTGTGTGT
    GAGCTCTGTG GCGAAGGGAA TCAGGAGACC AGTGAAGAGC TGATGGAGTG TTCCAACTGT
    GCTCAGATTG CACATCCCAG CTGTTTGAAG ACGTCTGGTG AGGGAGTGGT GAACAAAGAC
    CTGCCGAGCT GCTGGGAGTG TCCCAAGTGT GTTTTGGGAA AAGACACCGA TTCTGAGTCG
    TCAGGCAGCG GTGATGACAT CACGGCACAG GATGGGTCAG GGGGGCGAGG GGGCGAGGGC
    GGGGCGTGGC ATGGGGTCGG GCGGCCCCCC TCTTCTCTCT CTCACGGCTC GCTCCAGAAA
    CGGGCAGCAG CCCCTGAGCA GCGGCTCAGG AAGAGGATTA AACTGGATGG TGGTAAATCA
    CATAAGCGCA AGTCTTCATC TCTTGATCCC CGCGTGGCTA AAATCTACCG GAGACATGGG
    ATGGAGCACG ACGATGATTC AGGCAGCGAT GATGACGGAG ACGGTGGCAG AATGTCTTCA
    GTGCGCAGCA GCGTTTCCTC ATCTCGCAGA GGTTATGGAG CTAGCAGGCG AGGCGTTTGG
    AGAGGCTCCT CACAGCGGGG CCCTCGAGGG GGCGGAAGCC TGTCCTCAAG TTCCCTCAAA
    ATGAGGAGAG GACTCGGAGC AAGAGGAGAG AGAGGAGGTC GGGTGAGGTT GCGAGGAGGA
    TCGCAGATGA GGAGACGGCG GTATGAAACT GATGATGATG ATGATGACGA CGATGATGAT
    GATGATGACG ACGACGATGA TGATGACGAG GATGAGGATG ATGAGGATGA CAGCGAAGAA
    GACAGGCATG TGAGCCGGTC AGAGAAGGAG CATCGTTCCA GGAGACGAAG ACGAAGAGGT
    GATGATTACG ATGATGATGA GGACGAGGAT GACGATGATG AGGAGGACAG CGAAGAACAG
    GACTATGAGG GAGAGGACGA TGAAATGGAG GACTTGGATG ATGGAGGAGA TGAAGATGAT
    GAGGATGGAG AAAACCAATC AGACTCTGAT CCTGATCCAC CAGTTTTGCT CGTCTCAGAC
    TTGAACGATG ACCTGCTGAA CGATTCCTAC CTGACCGTGA CCCTCCATCG GCCGCCGAAG
    GCCAAACGTG ACCCTGGCTC AATTGTCCCT AAACTAGAGG CGGCGATGTC CCCCCGCACG
    GGTGGCAGTT CTGGCTTCAT GCAGCGAAAA ACTCTTCCCA AAGCGCGGCC CAAGACCAGC
    AGCTCCACCC CTGCTGGAAA CGGCCTCTCT CAGGCTAAAA GCGGCCGTCC CACGAGGCGA
    AGCTCCAACC AAGATGGCAG AGAGAGAGAC ACGGCGTCGC CCTCCTCCAT GTCGTCTCGC
    TCCTCTGTTT CTCCACCTCC TCCTCCTCCA GCTGTCACCT CTTCCTCTCC TTCCCTTCTC
    TCACATCCTT CATTCCGTGA TGCAGGAAAC GAGCTCGGCT GTGAAAAGGA CATTTGGGTG
    TCTGTGTTTC GTTACCTGGA TCGGACAGAT TTAGCTGTGT GCATGAGAGT CTGCAAGGCT
    TGGTTTAAAT GGTGTTGTGA TAAACGCTTA TGGACACGGA TAGATCTGAG TCGCTGTCGG
    GCTCTCAGTC CTCAGGCTGT GACGGCTATC ATTAAACTAC AACCTGTGAC CCTTGACCTC
    TCCTGGACAC CTGTGTCAAA GAAACAGCTG GCCTGGCTCA TTCATCACCT CCCAAGTTTA
    AAAGATCTAA TAATGTCCGG TTGTTCTTCA TTATGTGTGT CTGCATTAAG CTCGCCGAGC
    TGTCCGAGTT TGCGAACTCT TGACCTGTGT TGGGCTGTGG GCGTCAAAGA CTCGCAGATA
    AAAGATCTTA TTGTGCAGCC AGGCAGTGAA AGTCGCAGTC GTCTCAGAAG TTTGGTCAGT
    CTGCGCTTGT CTGGTTTGGA ATTGAGTGAC GCTGTCATTA AGACGATGGT CCGACACATG
    CCTTCACTGC GCCAGTTAGA TCTGTCCTAC TGTCAGGGCC TCACAGACCA GTCCATCAAC
    CTGCTCACTG CTACTGGATG CAACACTCGC AACACACTCA GACAGCTCAA TCTTTCAGGT
    TGTAATAAGC TGTCAGATGG CTGTCTCTCA TATATGAAGC GATTATCCGC TCTGGCTCTG
    TTGGATCTGC GGGGCTGCAA GAACGTGACC CGACATGGCT GCGAAAACTT CATCTCGGAC
    CTGTCCTACT GCACCGTATT CTGCCTGACC GAGGACAAAC TCATTCAGAG AGTATCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009291640.1
    CDS215..3934
    Translation

    Target ORF information:

    RefSeq Version XM_009293365.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio lysine (K)-specific demethylase 2Aa (kdm2aa), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009293365.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGAGGGCA AAGATTTCTC TTATGAGTAC GTCCAGAGGC ATGGTCTCCG GGATCCTATC 
    ATCTTTGAGA AACCCGAAGG TCTTGGCATC AAAATGCCAG ATCCAGACTT CACAGTAAAT
    GATGTGAAGT TGTTTGTAGG AAGTCGACGG ATAGTTGACG TGATGGACGT GGCTACTCAG
    AAAGGGACGG AGATGTCGAT GGCCCAGTGG AGGCGCTACT ACGAGACTCC TCCCTCTCAG
    AGAGAAAAGC TTTATAATGT AATCAGCCTT GAGTTTAGCC ACACTAAACT AGAGACCCTT
    GTCAAAAGAC CCTCCACGGT GGACATGATT GACTGGGTGG ACAACATGTG GCCTCGACAC
    TTAAAGGAAA GACAAAGAGA TTCTACAAAC TCTATCATGG AGATGCAATA CCCCAAAGTG
    CAAAAGTACT GCCTGATGAG TGTGCAGGGC TGTTACACAG ATTTCCACAT AGACTTTGGA
    GGCACGTCTG TGTGGTACCA CATACATAAA GGCTGCAAGG TGTTTTGGTT AATCCCTCCC
    ACGCCTCAAA ACCTGGAGCT TTATGAGAAC TGGGTGTTGT CAGGGAAACA AGGCGACATC
    TTTCTTGGTG ACAGGGCCAC TGATTGCCAG AGGATTGAGC TCAAGCAGGG CTACACGTTC
    ATCATTCCAT CAGGCTGGAT CCATGCTGTG TACACACCAG TGGACACGCT TGTCTTTGGT
    GGGAATTTTC TTCATAGTTT TAACGTCCCC ATGCAATTGG ACATCTCCGG CATTGAAGAC
    AGGACACGGG TGCCTGTGAA GTTCCGCTAT CCGTTCTTCT TTGAGATGTG CTGGTATGTG
    TTGGAGCGTT ATCTCTACAG CTTGACCAAC AAATCCCACC TCATCCCGGA GTTCCAGAAA
    CACTCACTGG GCATTGGCTT GAAGCGGGAA AACTCTGAGG TGCTGAATGG GCATGCAAAA
    GAGGAAAGAG ATGAGATGGA TGCCCCCTCT CCCCCTAGTG CACCAGGGTT AAAGACTCAC
    TTGACTGCTT TCGAGCTGGA AGGCCTGTGG AGGCTGGTAG GGAAGCTGGA ATCGCTGCCC
    TCAAATAAGA AGTGTGTGCC TTCAGGAATA CACAATGCTG CTGCCCTGCT GCTGGACATA
    CGGGCATTGT TAAAAGAACA TGCAAATGAC ATCCCTAAGC TCTCTTACAC TGGAAGACCC
    ATTGTCAGGT GGCCAAAGAG GCCTCCCTGG TACCAGCCCC CTCCTCCGCC TCCACCTGTC
    ATGCGTCCCA AGCTGGCGAC CACACCTATT GTCCCACGGC CTGTCAAACC AGCCTCCAAC
    ATGTCGGTCC TGCGCCGGCG CCGCGTGCGT TGCAAACGCT GCGAGGCCTG TCTGCGCACA
    GAATGTGGCG ACTGCAACTT CTGCCGAGAC ATGAAGAAAT TTGGCGGCCC TGGCAAGCTC
    AAACAGACCT GCGTCCTGCG GCAATGTCTT GCTCCTGGTC TGCCGCTCTC TGCTGTGTGT
    GAGCTCTGTG GCGAAGGGAA TCAGGAGACC AGTGAAGAGC TGATGGAGTG TTCCAACTGT
    GCTCAGATTG CACATCCCAG CTGTTTGAAG ACGTCTGGTG AGGGAGTGGT GAACAAAGAC
    CTGCCGAGCT GCTGGGAGTG TCCCAAGTGT GTTTTGGGAA AAGACACCGA TTCTGAGTCG
    TCAGGCAGCG GTGATGACAT CACGGCACAG GATGGGTCAG GGGGGCGAGG GGGCGAGGGC
    GGGGCGTGGC ATGGGGTCGG GCGGCCCCCC TCTTCTCTCT CTCACGGCTC GCTCCAGAAA
    CGGGCAGCAG CCCCTGAGCA GCGGCTCAGG AAGAGGATTA AACTGGATGG TGGTAAATCA
    CATAAGCGCA AGTCTTCATC TCTTGATCCC CGCGTGGCTA AAATCTACCG GAGACATGGG
    ATGGAGCACG ACGATGATTC AGGCAGCGAT GATGACGGAG ACGGTGGCAG AATGTCTTCA
    GTGCGCAGCA GCGTTTCCTC ATCTCGCAGA GGTTATGGAG CTAGCAGGCG AGGCGTTTGG
    AGAGGCTCCT CACAGCGGGG CCCTCGAGGG GGCGGAAGCC TGTCCTCAAG TTCCCTCAAA
    ATGAGGAGAG GACTCGGAGC AAGAGGAGAG AGAGGAGGTC GGGTGAGGTT GCGAGGAGGA
    TCGCAGATGA GGAGACGGCG GTATGAAACT GATGATGATG ATGATGACGA CGATGATGAT
    GATGATGACG ACGACGATGA TGATGACGAG GATGAGGATG ATGAGGATGA CAGCGAAGAA
    GACAGGCATG TGAGCCGGTC AGAGAAGGAG CATCGTTCCA GGAGACGAAG ACGAAGAGGT
    GATGATTACG ATGATGATGA GGACGAGGAT GACGATGATG AGGAGGACAG CGAAGAACAG
    GACTATGAGG GAGAGGACGA TGAAATGGAG GACTTGGATG ATGGAGGAGA TGAAGATGAT
    GAGGATGGAG AAAACCAATC AGACTCTGAT CCTGATCCAC CAGTTTTGCT CGTCTCAGAC
    TTGAACGATG ACCTGCTGAA CGATTCCTAC CTGACCGTGA CCCTCCATCG GCCGCCGAAG
    GCCAAACGTG ACCCTGGCTC AATTGTCCCT AAACTAGAGG CGGCGATGTC CCCCCGCACG
    GGTGGCAGTT CTGGCTTCAT GCAGCGAAAA ACTCTTCCCA AAGCGCGGCC CAAGACCAGC
    AGCTCCACCC CTGCTGGAAA CGGCCTCTCT CAGGCTAAAA GCGGCCGTCC CACGAGGCGA
    AGCTCCAACC AAGATGGCAG AGAGAGAGAC ACGGCGTCGC CCTCCTCCAT GTCGTCTCGC
    TCCTCTGTTT CTCCACCTCC TCCTCCTCCA GCTGTCACCT CTTCCTCTCC TTCCCTTCTC
    TCACATCCTT CATTCCGTGA TGCAGGAAAC GAGCTCGGCT GTGAAAAGGA CATTTGGGTG
    TCTGTGTTTC GTTACCTGGA TCGGACAGAT TTAGCTGTGT GCATGAGAGT CTGCAAGGCT
    TGGTTTAAAT GGTGTTGTGA TAAACGCTTA TGGACACGGA TAGATCTGAG TCGCTGTCGG
    GCTCTCAGTC CTCAGGCTGT GACGGCTATC ATTAAACTAC AACCTGTGAC CCTTGACCTC
    TCCTGGACAC CTGTGTCAAA GAAACAGCTG GCCTGGCTCA TTCATCACCT CCCAAGTTTA
    AAAGATCTAA TAATGTCCGG TTGTTCTTCA TTATGTGTGT CTGCATTAAG CTCGCCGAGC
    TGTCCGAGTT TGCGAACTCT TGACCTGTGT TGGGCTGTGG GCGTCAAAGA CTCGCAGATA
    AAAGATCTTA TTGTGCAGCC AGGCAGTGAA AGTCGCAGTC GTCTCAGAAG TTTGGTCAGT
    CTGCGCTTGT CTGGTTTGGA ATTGAGTGAC GCTGTCATTA AGACGATGGT CCGACACATG
    CCTTCACTGC GCCAGTTAGA TCTGTCCTAC TGTCAGGGCC TCACAGACCA GTCCATCAAC
    CTGCTCACTG CTACTGGATG CAACACTCGC AACACACTCA GACAGCTCAA TCTTTCAGGT
    TGTAATAAGC TGTCAGATGG CTGTCTCTCA TATATGAAGC GATTATCCGC TCTGGCTCTG
    TTGGATCTGC GGGGCTGCAA GAACGTGACC CGACATGGCT GCGAAAACTT CATCTCGGAC
    CTGTCCTACT GCACCGTATT CTGCCTGACC GAGGACAAAC TCATTCAGAG AGTATCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.