kank2 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	kank2
Entrez Gene ID	571549
Full Name	KN motif and ankyrin repeat domains 2
Synonyms	dZ118J2.12,si:busm1-118j2.12,si:ch73-108m21.1
General protein information	Preferred Names KN motif and ankyrin repeat domains 2 Names si:ch73-108m21.1 dZ118J2.12 (novel protein)
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005164339.3	XP_005164396.1	si:ch73-108m21.1 isoform X1
XM_005164339.4	XP_005164396.1	si:ch73-108m21.1 isoform X1
XM_021471168.1	XP_021326843.1	si:ch73-108m21.1 isoform X1
NM_001305620.1	NP_001292549.1	KN motif and ankyrin repeat domain-containing protein 2

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	KANK2	XP_003432823.1
Rattus norvegicus (Norway rat)	Kank2	NP_001257342.1
Pan troglodytes (chimpanzee)	KANK2	XP_003316135.1
Bos taurus (cattle)	KANK2	NP_001069999.1
Mus musculus (house mouse)	Kank2	NP_663586.3
Homo sapiens (human)	KANK2	NP_001129663.1
Danio rerio (zebrafish)	LOC571549	XP_005164397.1
Xenopus tropicalis (tropical clawed frog)	kank2	XP_002942889.2

Gene Ontology
Bibliography

Related articles in PubMed

Evolutionary and developmental analysis reveals KANK genes were co-opted for vertebrate vascular development.
Hensley MR, Cui Z, Chua RF, Simpson S, Shammas NL, Yang JY, Leung YF, Zhang G
Scientific reports627816(2016 Jun)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

KANK deficiency leads to podocyte dysfunction and nephrotic syndrome.
Gee HY, Zhang F, Ashraf S, Kohl S, Sadowski CE, Vega-Warner V, Zhou W, Lovric S, Fang H, Nettleton M, Zhu JY, Hoefele J, Weber LT, Podracka L, Boor A, Fehrenbach H, Innis JW, Washburn J, Levy S, Lifton RP, Otto EA, Han Z, Hildebrandt F
The Journal of clinical investigation125(6)2375-84(2015 Jun)

The timing of Timezyme diversification in vertebrates.
Cazam?a-Catalan D, Besseau L, Falc?n J, Magnanou E
PloS one9(12)e112380(2014)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Knockdown of kank2 in zebrafish recapitulated a nephrotic syndrome phenotype, resulting in proteinuria and podocyte foot process effacement
Title: KANK deficiency leads to podocyte dysfunction and nephrotic syndrome.

The following kank2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the kank2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa08860	NM_001305620.1 Latest version!	Danio rerio KN motif and ankyrin repeat domains 2 (kank2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa08860	XM_005164339.4 Latest version!	Danio rerio KN motif and ankyrin repeat domains 2 (kank2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa08860	XM_021471168.1 Latest version!	Danio rerio KN motif and ankyrin repeat domains 2 (kank2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
View Old Accession Versions >>
ODa08860	XM_005164339.3	Danio rerio KN motif and ankyrin repeat domains 2 (kank2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

kank2 ( NM_001305620.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001292549.1 Danio rerio KN motif and ankyrin repeat domains 2 (kank2), mRNA.

Price & Availability↑

CloneID	ODa08860
Clone ID Related Accession (Same CDS sequence)	XM_005164339.3 , XM_005164339.4 , XM_021471168.1 , NM_001305620.1
Accession Version	NM_001305620.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3258bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-04
Organism	Danio rerio(zebrafish)
Product	KN motif and ankyrin repeat domain-containing protein 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CU464181.10. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## CDS exon combination :: GDQH01013815.1, GFIL01024467.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGACAATCA TGGCTCAGGT GCTTCATATG GACTCCAGTT TTCCTGGGAA GATTAACCCC 
CCGGTGCCCC CCTCCTTGCA TGCAAAGGAC CAAGAGGCTC CATATTCAGT AGAAACTCCT 
TACGGATACC GTCTGGACCT TGACTTTCTC AAGTATGTCA ATGATATTGA GAAAGGAAAC 
ACTATTAAGA AGGTGCCAGT GCAGCGCCGC CCACGTTATG GCTCGCTTCC ACGTGGCTAC 
GGCTACACAG GCTCCTGGTG GACCTCCACA GAGTCCCTGT GCTCCAATGC CAGCATGGAC 
AGCCGGCACT CATCATATTC TTACTGTGCA CCAGGCTTCC ACACCTCGCA GAGACCCAAC 
TTCAGTACAG CACGTGTAGA GAAAACCCTA ATGGATGCTC GCCGGAAGCT GGAGGAAGAG 
AAAGATGGTC GCCGTTTCTC AAATTTGGGC AGCATGCACA GCAGTATGGC TGGCTCTAAT 
ACATCTCTGT CCAGTGCGCA CAGCTTTAAT CGTGCCCAAG GAGGGGGCTC CTATACACCA 
ATGAGCTCCG GCTTGTCCAC ACCTGTCTCC CCGACGCCAG CTCACCTGCA GCACGTGCGG 
GAGCAGATGG CCGTTGCCCT CCGGAAGATC CGGGAGCTGG AAGAACAAGT GAAAACCATC 
CCAGTGCTGC AGGTCAAGAT CTCTGTACTT CAAGAGGAGA AGAGGCAGCT CAGTGTGCAA 
CTTAAGAGTC AGAAGTTCCT GGGACACACG CTGGGGTTCA ATCGAAGCCG TCCACGAGGC 
GAACTGTACA TCGACATACC TGAGGAGGAG GCAGGAAACG GGGCAGGAGC TGCAAACAAA 
GCCACAGGGA GTCTTTCACC CACCACTCCC GGCTCCCTCC AGGACTCAGG ATGTGAAATA 
GAGGAGACTG TAATTGTTGC GGGGGCTCGT CCGGGTGCAA AGCGGGAGGT GCGAACTGTA 
GGTGTGGGGC CTGAAGCAGA AGAAAGGGGT TATCGTCAAG TGGGGGTAGG GGTGCGAGAG 
CAGGACTTGG GTCTGCTGCC TGAAACCGAA GCCCTCAAGA CTAAAGTGGG TCTTTTAGAA 
GTTCAGCTGA GGAAAACCAT GCAAGAGCTA CAGAGTGCTC AGCAGCAAGT GGAAGCGGCA 
CAGAAAGAGA GGCAGACAGT TTGCCCTCAG GTAGACCATG CTGTGAGAGC CACCAGTCTC 
GGCTGGCAGG ATCAGCAGGG CCAGGCTGGA GCGGGCTTGC ATACTGTTGT CAGTTTTACT 
AAGCAACCTC ATCAGCAGAG GACTGTGGGC ATCCAAGTGT ACACGCTGGA GCAGCCCACA 
GTGGTGGGTG TGGGGACACT GCTAAGAGCC CAGGGCTGCA CTCATCCTGC TCAACTGGAG 
GCGTCTCACA GAAGATATGG AGAGTCCCCA GCAGCAGGGG ATTCGCCACA TGAGTTTCCC 
ATTGCAATAA GCTCTAAGCA GGTGCGGGAG GTCTTGAGAA GTGAGGTCTC CAAATCAGTG 
CCAGTGACCA ACCAGGCAAC TCCTATGGAG ACAAAGTGCA ACCAAGTGAC TGCGGTCTGC 
CAACGGCTAA AAGAAGGAGA GATTAACCAG CAACCAGCAG AAGAAGCCAT TCAAGTTGAT 
CCAGCCAATC CAGCATCCCC TCAGTCTAAC CTGAGGTCAA TCATGAAGAG GAAGGCTGAT 
GGGGAACCTG GCTCTCCATA CACCAAAAAG AACCTGCAGT TCTTAGGAGT CAATGGAGGG 
TACGAGTCAA CATCCTCCGA AGAGAGCAGC TCTGAAAGTT CAGAAGATGA AAGCGATGCA 
AGCGAATACC ATGAGGCTAC GGAAAAACTA CCGGAATCAG CGACACCACA GTCTCTGGTG 
TCCTCCTGTA TCCCACAGCT CGCCTCTGAG ACACCAGCTA CCCAAACAGC TCAGCACAGC 
ACTGCCCAGA TACCAACCAA TCACACTCCA GCAGCTCAAA CCACCAGCCA ATCACACACC 
ACAGATGCAA CTACCCAACA ACATGTCACC CAATCACCAG CAGCAGAGGC TGATGTACAG 
CACTGTGTCA GCCAATCATC AGTGACCACC ACACCTCCTC CAGAGGGAGA TGTTCAATGT 
GTTTTTCAAG GGTGTAACCC TCCAAGCACT GCCACTTCTC TTGAACAAAA CTCTGTCCAG 
TTGTCATCTG CGACGCAGCA GTCCAAAGCC ACTGATTCAA ACGTCCAGCC AGATCTCGCT 
CAAACAGATG TGTCGGACTT CGCCGCTCAG CAAACGACCA CTCAAACACA ATTCACCAGT 
GCCAAACCTC AGCAAGAAGC TAGCCAATCA AAAACTGCTG ACCTCACAGC CCAGAAAGGA 
GCGACACACT CTACTGATGG CTCAGCGAAA CAGGACATAG CCATATCCTC CACAACTAAA 
CCAGCAGCAG ATACAGCAAC ACCACCCACT AATAAACAAA CAGACAGCCT GGAATTAAGT 
GGCGGTCTTA TGTCGGCGCT CCACATCTTG CAGAAGGCGC TCAGCGAACC AAATGCATTC 
AGCCATCAAG ATGCGAGGAC GGCCTACACC AGCGTCCTGC AGGAGTGGCT CCGGGTCTCC 
TGTCATAAAG CTGCTGACAC CGCAGTGGTG AAGGCCCACA TGGACGCGTT CGCTTCTATT 
TCCCCTCAGC TCCTGGAGTT CGTCATCAAC ATGGCCGACG GCAATGGAAA CACGGCTCTG 
CACTACACCG TCTCTCACTC CAACTTCCCT GTAGTGAAGC TGCTGCTGGA CACCGGTCTC 
TGTAATGCTG ACAAGCAGAA CAAGGCTGGT TACACAGCCA TCATGTTGAC GGCTCTGGCT 
GCCTTCAGCT CGGACAGTGA CCTTCAGACC GTCCTACAGC TGCTCCGCAC CGGCGACGTC 
AATGCCAAAG CCAGCCAGGC AGGGCAGACG GCACTGATGC TGGCAGTCAG TCATGGTCGA 
GGGGATATGG TGAAGGCTCT GCTGGCATGT GGAGCTCAGG TGAACCTGCG GGATGACGAC 
GGCTCCACAG CGCTCATGTG CGCCTGTGAA CATGGACATG TGGACATCGT GCGCCAGCTG 
CTGTCTGTGC CCGGCTGTGA CGCCACGCTC ACTGATAATG ATGGCAGCAC TGCTCTCTCC 
ATCGCGTTGG AGGCCAGTCA GAATGACATT GCTGTGCTTC TATATGCACA CCTCAATTTT 
GCCAAGCCGC CTTCTCCTGT ATCACCAAAG TCTCCAATTC TGGGCTCTTC TCCTCCTTCG 
TCCTCTGAAC TGAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001292549.1
CDS	168..3425
Translation	MTIMAQVLHMDSSFPGKINPPVPPSLHAKDQEAPYSVETPYGYRLDLDFLKYVNDIEKGNTIKKVPVQRRPRYGSLPRGYGYTGSWWTSTESLCSNASMDSRHSSYSYCAPGFHTSQRPNFSTARVEKTLMDARRKLEEEKDGRRFSNLGSMHSSMAGSNTSLSSAHSFNRAQGGGSYTPMSSGLSTPVSPTPAHLQHVREQMAVALRKIRELEEQVKTIPVLQVKISVLQEEKRQLSVQLKSQKFLGHTLGFNRSRPRGELYIDIPEEEAGNGAGAANKATGSLSPTTPGSLQDSGCEIEETVIVAGARPGAKREVRTVGVGPEAEERGYRQVGVGVREQDLGLLPETEALKTKVGLLEVQLRKTMQELQSAQQQVEAAQKERQTVCPQVDHAVRATSLGWQDQQGQAGAGLHTVVSFTKQPHQQRTVGIQVYTLEQPTVVGVGTLLRAQGCTHPAQLEASHRRYGESPAAGDSPHEFPIAISSKQVREVLRSEVSKSVPVTNQATPMETKCNQVTAVCQRLKEGEINQQPAEEAIQVDPANPASPQSNLRSIMKRKADGEPGSPYTKKNLQFLGVNGGYESTSSEESSSESSEDESDASEYHEATEKLPESATPQSLVSSCIPQLASETPATQTAQHSTAQIPTNHTPAAQTTSQSHTTDATTQQHVTQSPAAEADVQHCVSQSSVTTTPPPEGDVQCVFQGCNPPSTATSLEQNSVQLSSATQQSKATDSNVQPDLAQTDVSDFAAQQTTTQTQFTSAKPQQEASQSKTADLTAQKGATHSTDGSAKQDIAISSTTKPAADTATPPTNKQTDSLELSGGLMSALHILQKALSEPNAFSHQDARTAYTSVLQEWLRVSCHKAADTAVVKAHMDAFASISPQLLEFVINMADGNGNTALHYTVSHSNFPVVKLLLDTGLCNADKQNKAGYTAIMLTALAAFSSDSDLQTVLQLLRTGDVNAKASQAGQTALMLAVSHGRGDMVKALLACGAQVNLRDDDGSTALMCACEHGHVDIVRQLLSVPGCDATLTDNDGSTALSIALEASQNDIAVLLYAHLNFAKPPSPVSPKSPILGSSPPSSSELK

Target ORF information:

RefSeq Version	NM_001305620.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio KN motif and ankyrin repeat domains 2 (kank2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001305620.1

ORF Insert Sequence:

ATGACAATCA TGGCTCAGGT GCTTCATATG GACTCCAGTT TTCCTGGGAA GATTAACCCC 
CCGGTGCCCC CCTCCTTGCA TGCAAAGGAC CAAGAGGCTC CATATTCAGT AGAAACTCCT 
TACGGATACC GTCTGGACCT TGACTTTCTC AAGTATGTCA ATGATATTGA GAAAGGAAAC 
ACTATTAAGA AGGTGCCAGT GCAGCGCCGC CCACGTTATG GCTCGCTTCC ACGTGGCTAC 
GGCTACACAG GCTCCTGGTG GACCTCCACA GAGTCCCTGT GCTCCAATGC CAGCATGGAC 
AGCCGGCACT CATCATATTC TTACTGTGCA CCAGGCTTCC ACACCTCGCA GAGACCCAAC 
TTCAGTACAG CACGTGTAGA GAAAACCCTA ATGGATGCTC GCCGGAAGCT GGAGGAAGAG 
AAAGATGGTC GCCGTTTCTC AAATTTGGGC AGCATGCACA GCAGTATGGC TGGCTCTAAT 
ACATCTCTGT CCAGTGCGCA CAGCTTTAAT CGTGCCCAAG GAGGGGGCTC CTATACACCA 
ATGAGCTCCG GCTTGTCCAC ACCTGTCTCC CCGACGCCAG CTCACCTGCA GCACGTGCGG 
GAGCAGATGG CCGTTGCCCT CCGGAAGATC CGGGAGCTGG AAGAACAAGT GAAAACCATC 
CCAGTGCTGC AGGTCAAGAT CTCTGTACTT CAAGAGGAGA AGAGGCAGCT CAGTGTGCAA 
CTTAAGAGTC AGAAGTTCCT GGGACACACG CTGGGGTTCA ATCGAAGCCG TCCACGAGGC 
GAACTGTACA TCGACATACC TGAGGAGGAG GCAGGAAACG GGGCAGGAGC TGCAAACAAA 
GCCACAGGGA GTCTTTCACC CACCACTCCC GGCTCCCTCC AGGACTCAGG ATGTGAAATA 
GAGGAGACTG TAATTGTTGC GGGGGCTCGT CCGGGTGCAA AGCGGGAGGT GCGAACTGTA 
GGTGTGGGGC CTGAAGCAGA AGAAAGGGGT TATCGTCAAG TGGGGGTAGG GGTGCGAGAG 
CAGGACTTGG GTCTGCTGCC TGAAACCGAA GCCCTCAAGA CTAAAGTGGG TCTTTTAGAA 
GTTCAGCTGA GGAAAACCAT GCAAGAGCTA CAGAGTGCTC AGCAGCAAGT GGAAGCGGCA 
CAGAAAGAGA GGCAGACAGT TTGCCCTCAG GTAGACCATG CTGTGAGAGC CACCAGTCTC 
GGCTGGCAGG ATCAGCAGGG CCAGGCTGGA GCGGGCTTGC ATACTGTTGT CAGTTTTACT 
AAGCAACCTC ATCAGCAGAG GACTGTGGGC ATCCAAGTGT ACACGCTGGA GCAGCCCACA 
GTGGTGGGTG TGGGGACACT GCTAAGAGCC CAGGGCTGCA CTCATCCTGC TCAACTGGAG 
GCGTCTCACA GAAGATATGG AGAGTCCCCA GCAGCAGGGG ATTCGCCACA TGAGTTTCCC 
ATTGCAATAA GCTCTAAGCA GGTGCGGGAG GTCTTGAGAA GTGAGGTCTC CAAATCAGTG 
CCAGTGACCA ACCAGGCAAC TCCTATGGAG ACAAAGTGCA ACCAAGTGAC TGCGGTCTGC 
CAACGGCTAA AAGAAGGAGA GATTAACCAG CAACCAGCAG AAGAAGCCAT TCAAGTTGAT 
CCAGCCAATC CAGCATCCCC TCAGTCTAAC CTGAGGTCAA TCATGAAGAG GAAGGCTGAT 
GGGGAACCTG GCTCTCCATA CACCAAAAAG AACCTGCAGT TCTTAGGAGT CAATGGAGGG 
TACGAGTCAA CATCCTCCGA AGAGAGCAGC TCTGAAAGTT CAGAAGATGA AAGCGATGCA 
AGCGAATACC ATGAGGCTAC GGAAAAACTA CCGGAATCAG CGACACCACA GTCTCTGGTG 
TCCTCCTGTA TCCCACAGCT CGCCTCTGAG ACACCAGCTA CCCAAACAGC TCAGCACAGC 
ACTGCCCAGA TACCAACCAA TCACACTCCA GCAGCTCAAA CCACCAGCCA ATCACACACC 
ACAGATGCAA CTACCCAACA ACATGTCACC CAATCACCAG CAGCAGAGGC TGATGTACAG 
CACTGTGTCA GCCAATCATC AGTGACCACC ACACCTCCTC CAGAGGGAGA TGTTCAATGT 
GTTTTTCAAG GGTGTAACCC TCCAAGCACT GCCACTTCTC TTGAACAAAA CTCTGTCCAG 
TTGTCATCTG CGACGCAGCA GTCCAAAGCC ACTGATTCAA ACGTCCAGCC AGATCTCGCT 
CAAACAGATG TGTCGGACTT CGCCGCTCAG CAAACGACCA CTCAAACACA ATTCACCAGT 
GCCAAACCTC AGCAAGAAGC TAGCCAATCA AAAACTGCTG ACCTCACAGC CCAGAAAGGA 
GCGACACACT CTACTGATGG CTCAGCGAAA CAGGACATAG CCATATCCTC CACAACTAAA 
CCAGCAGCAG ATACAGCAAC ACCACCCACT AATAAACAAA CAGACAGCCT GGAATTAAGT 
GGCGGTCTTA TGTCGGCGCT CCACATCTTG CAGAAGGCGC TCAGCGAACC AAATGCATTC 
AGCCATCAAG ATGCGAGGAC GGCCTACACC AGCGTCCTGC AGGAGTGGCT CCGGGTCTCC 
TGTCATAAAG CTGCTGACAC CGCAGTGGTG AAGGCCCACA TGGACGCGTT CGCTTCTATT 
TCCCCTCAGC TCCTGGAGTT CGTCATCAAC ATGGCCGACG GCAATGGAAA CACGGCTCTG 
CACTACACCG TCTCTCACTC CAACTTCCCT GTAGTGAAGC TGCTGCTGGA CACCGGTCTC 
TGTAATGCTG ACAAGCAGAA CAAGGCTGGT TACACAGCCA TCATGTTGAC GGCTCTGGCT 
GCCTTCAGCT CGGACAGTGA CCTTCAGACC GTCCTACAGC TGCTCCGCAC CGGCGACGTC 
AATGCCAAAG CCAGCCAGGC AGGGCAGACG GCACTGATGC TGGCAGTCAG TCATGGTCGA 
GGGGATATGG TGAAGGCTCT GCTGGCATGT GGAGCTCAGG TGAACCTGCG GGATGACGAC 
GGCTCCACAG CGCTCATGTG CGCCTGTGAA CATGGACATG TGGACATCGT GCGCCAGCTG 
CTGTCTGTGC CCGGCTGTGA CGCCACGCTC ACTGATAATG ATGGCAGCAC TGCTCTCTCC 
ATCGCGTTGG AGGCCAGTCA GAATGACATT GCTGTGCTTC TATATGCACA CCTCAATTTT 
GCCAAGCCGC CTTCTCCTGT ATCACCAAAG TCTCCAATTC TGGGCTCTTC TCCTCCTTCG 
TCCTCTGAAC TGAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kank2 ( XM_005164339.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005164396.1 Danio rerio KN motif and ankyrin repeat domains 2 (kank2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa08860
Clone ID Related Accession (Same CDS sequence)	XM_005164339.3 , XM_005164339.4 , XM_021471168.1 , NM_001305620.1
Accession Version	XM_005164339.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3258bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	si:ch73-108m21.1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005164339.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACAATCA TGGCTCAGGT GCTTCATATG GACTCCAGTT TTCCTGGGAA GATTAACCCC 
CCGGTGCCCC CCTCCTTGCA TGCAAAGGAC CAAGAGGCTC CATATTCAGT AGAAACTCCT 
TACGGATACC GTCTGGACCT TGACTTTCTC AAGTATGTCA ATGATATTGA GAAAGGAAAC 
ACTATTAAGA AGGTGCCAGT GCAGCGCCGC CCACGTTATG GCTCGCTTCC ACGTGGCTAC 
GGCTACACAG GCTCCTGGTG GACCTCCACA GAGTCCCTGT GCTCCAATGC CAGCATGGAC 
AGCCGGCACT CATCATATTC TTACTGTGCA CCAGGCTTCC ACACCTCGCA GAGACCCAAC 
TTCAGTACAG CACGTGTAGA GAAAACCCTA ATGGATGCTC GCCGGAAGCT GGAGGAAGAG 
AAAGATGGTC GCCGTTTCTC AAATTTGGGC AGCATGCACA GCAGTATGGC TGGCTCTAAT 
ACATCTCTGT CCAGTGCGCA CAGCTTTAAT CGTGCCCAAG GAGGGGGCTC CTATACACCA 
ATGAGCTCCG GCTTGTCCAC ACCTGTCTCC CCGACGCCAG CTCACCTGCA GCACGTGCGG 
GAGCAGATGG CCGTTGCCCT CCGGAAGATC CGGGAGCTGG AAGAACAAGT GAAAACCATC 
CCAGTGCTGC AGGTCAAGAT CTCTGTACTT CAAGAGGAGA AGAGGCAGCT CAGTGTGCAA 
CTTAAGAGTC AGAAGTTCCT GGGACACACG CTGGGGTTCA ATCGAAGCCG TCCACGAGGC 
GAACTGTACA TCGACATACC TGAGGAGGAG GCAGGAAACG GGGCAGGAGC TGCAAACAAA 
GCCACAGGGA GTCTTTCACC CACCACTCCC GGCTCCCTCC AGGACTCAGG ATGTGAAATA 
GAGGAGACTG TAATTGTTGC GGGGGCTCGT CCGGGTGCAA AGCGGGAGGT GCGAACTGTA 
GGTGTGGGGC CTGAAGCAGA AGAAAGGGGT TATCGTCAAG TGGGGGTAGG GGTGCGAGAG 
CAGGACTTGG GTCTGCTGCC TGAAACCGAA GCCCTCAAGA CTAAAGTGGG TCTTTTAGAA 
GTTCAGCTGA GGAAAACCAT GCAAGAGCTA CAGAGTGCTC AGCAGCAAGT GGAAGCGGCA 
CAGAAAGAGA GGCAGACAGT TTGCCCTCAG GTAGACCATG CTGTGAGAGC CACCAGTCTC 
GGCTGGCAGG ATCAGCAGGG CCAGGCTGGA GCGGGCTTGC ATACTGTTGT CAGTTTTACT 
AAGCAACCTC ATCAGCAGAG GACTGTGGGC ATCCAAGTGT ACACGCTGGA GCAGCCCACA 
GTGGTGGGTG TGGGGACACT GCTAAGAGCC CAGGGCTGCA CTCATCCTGC TCAACTGGAG 
GCGTCTCACA GAAGATATGG AGAGTCCCCA GCAGCAGGGG ATTCGCCACA TGAGTTTCCC 
ATTGCAATAA GCTCTAAGCA GGTGCGGGAG GTCTTGAGAA GTGAGGTCTC CAAATCAGTG 
CCAGTGACCA ACCAGGCAAC TCCTATGGAG ACAAAGTGCA ACCAAGTGAC TGCGGTCTGC 
CAACGGCTAA AAGAAGGAGA GATTAACCAG CAACCAGCAG AAGAAGCCAT TCAAGTTGAT 
CCAGCCAATC CAGCATCCCC TCAGTCTAAC CTGAGGTCAA TCATGAAGAG GAAGGCTGAT 
GGGGAACCTG GCTCTCCATA CACCAAAAAG AACCTGCAGT TCTTAGGAGT CAATGGAGGG 
TACGAGTCAA CATCCTCCGA AGAGAGCAGC TCTGAAAGTT CAGAAGATGA AAGCGATGCA 
AGCGAATACC ATGAGGCTAC GGAAAAACTA CCGGAATCAG CGACACCACA GTCTCTGGTG 
TCCTCCTGTA TCCCACAGCT CGCCTCTGAG ACACCAGCTA CCCAAACAGC TCAGCACAGC 
ACTGCCCAGA TACCAACCAA TCACACTCCA GCAGCTCAAA CCACCAGCCA ATCACACACC 
ACAGATGCAA CTACCCAACA ACATGTCACC CAATCACCAG CAGCAGAGGC TGATGTACAG 
CACTGTGTCA GCCAATCATC AGTGACCACC ACACCTCCTC CAGAGGGAGA TGTTCAATGT 
GTTTTTCAAG GGTGTAACCC TCCAAGCACT GCCACTTCTC TTGAACAAAA CTCTGTCCAG 
TTGTCATCTG CGACGCAGCA GTCCAAAGCC ACTGATTCAA ACGTCCAGCC AGATCTCGCT 
CAAACAGATG TGTCGGACTT CGCCGCTCAG CAAACGACCA CTCAAACACA ATTCACCAGT 
GCCAAACCTC AGCAAGAAGC TAGCCAATCA AAAACTGCTG ACCTCACAGC CCAGAAAGGA 
GCGACACACT CTACTGATGG CTCAGCGAAA CAGGACATAG CCATATCCTC CACAACTAAA 
CCAGCAGCAG ATACAGCAAC ACCACCCACT AATAAACAAA CAGACAGCCT GGAATTAAGT 
GGCGGTCTTA TGTCGGCGCT CCACATCTTG CAGAAGGCGC TCAGCGAACC AAATGCATTC 
AGCCATCAAG ATGCGAGGAC GGCCTACACC AGCGTCCTGC AGGAGTGGCT CCGGGTCTCC 
TGTCATAAAG CTGCTGACAC CGCAGTGGTG AAGGCCCACA TGGACGCGTT CGCTTCTATT 
TCCCCTCAGC TCCTGGAGTT CGTCATCAAC ATGGCCGACG GCAATGGAAA CACGGCTCTG 
CACTACACCG TCTCTCACTC CAACTTCCCT GTAGTGAAGC TGCTGCTGGA CACCGGTCTC 
TGTAATGCTG ACAAGCAGAA CAAGGCTGGT TACACAGCCA TCATGTTGAC GGCTCTGGCT 
GCCTTCAGCT CGGACAGTGA CCTTCAGACC GTCCTACAGC TGCTCCGCAC CGGCGACGTC 
AATGCCAAAG CCAGCCAGGC AGGGCAGACG GCACTGATGC TGGCAGTCAG TCATGGTCGA 
GGGGATATGG TGAAGGCTCT GCTGGCATGT GGAGCTCAGG TGAACCTGCG GGATGACGAC 
GGCTCCACAG CGCTCATGTG CGCCTGTGAA CATGGACATG TGGACATCGT GCGCCAGCTG 
CTGTCTGTGC CCGGCTGTGA CGCCACGCTC ACTGATAATG ATGGCAGCAC TGCTCTCTCC 
ATCGCGTTGG AGGCCAGTCA GAATGACATT GCTGTGCTTC TATATGCACA CCTCAATTTT 
GCCAAGCCGC CTTCTCCTGT ATCACCAAAG TCTCCAATTC TGGGCTCTTC TCCTCCTTCG 
TCCTCTGAAC TGAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005164396.1
CDS	194..3451
Translation	MTIMAQVLHMDSSFPGKINPPVPPSLHAKDQEAPYSVETPYGYRLDLDFLKYVNDIEKGNTIKKVPVQRRPRYGSLPRGYGYTGSWWTSTESLCSNASMDSRHSSYSYCAPGFHTSQRPNFSTARVEKTLMDARRKLEEEKDGRRFSNLGSMHSSMAGSNTSLSSAHSFNRAQGGGSYTPMSSGLSTPVSPTPAHLQHVREQMAVALRKIRELEEQVKTIPVLQVKISVLQEEKRQLSVQLKSQKFLGHTLGFNRSRPRGELYIDIPEEEAGNGAGAANKATGSLSPTTPGSLQDSGCEIEETVIVAGARPGAKREVRTVGVGPEAEERGYRQVGVGVREQDLGLLPETEALKTKVGLLEVQLRKTMQELQSAQQQVEAAQKERQTVCPQVDHAVRATSLGWQDQQGQAGAGLHTVVSFTKQPHQQRTVGIQVYTLEQPTVVGVGTLLRAQGCTHPAQLEASHRRYGESPAAGDSPHEFPIAISSKQVREVLRSEVSKSVPVTNQATPMETKCNQVTAVCQRLKEGEINQQPAEEAIQVDPANPASPQSNLRSIMKRKADGEPGSPYTKKNLQFLGVNGGYESTSSEESSSESSEDESDASEYHEATEKLPESATPQSLVSSCIPQLASETPATQTAQHSTAQIPTNHTPAAQTTSQSHTTDATTQQHVTQSPAAEADVQHCVSQSSVTTTPPPEGDVQCVFQGCNPPSTATSLEQNSVQLSSATQQSKATDSNVQPDLAQTDVSDFAAQQTTTQTQFTSAKPQQEASQSKTADLTAQKGATHSTDGSAKQDIAISSTTKPAADTATPPTNKQTDSLELSGGLMSALHILQKALSEPNAFSHQDARTAYTSVLQEWLRVSCHKAADTAVVKAHMDAFASISPQLLEFVINMADGNGNTALHYTVSHSNFPVVKLLLDTGLCNADKQNKAGYTAIMLTALAAFSSDSDLQTVLQLLRTGDVNAKASQAGQTALMLAVSHGRGDMVKALLACGAQVNLRDDDGSTALMCACEHGHVDIVRQLLSVPGCDATLTDNDGSTALSIALEASQNDIAVLLYAHLNFAKPPSPVSPKSPILGSSPPSSSELK

Target ORF information:

RefSeq Version	XM_005164339.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio KN motif and ankyrin repeat domains 2 (kank2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005164339.3

ORF Insert Sequence:

ATGACAATCA TGGCTCAGGT GCTTCATATG GACTCCAGTT TTCCTGGGAA GATTAACCCC 
CCGGTGCCCC CCTCCTTGCA TGCAAAGGAC CAAGAGGCTC CATATTCAGT AGAAACTCCT 
TACGGATACC GTCTGGACCT TGACTTTCTC AAGTATGTCA ATGATATTGA GAAAGGAAAC 
ACTATTAAGA AGGTGCCAGT GCAGCGCCGC CCACGTTATG GCTCGCTTCC ACGTGGCTAC 
GGCTACACAG GCTCCTGGTG GACCTCCACA GAGTCCCTGT GCTCCAATGC CAGCATGGAC 
AGCCGGCACT CATCATATTC TTACTGTGCA CCAGGCTTCC ACACCTCGCA GAGACCCAAC 
TTCAGTACAG CACGTGTAGA GAAAACCCTA ATGGATGCTC GCCGGAAGCT GGAGGAAGAG 
AAAGATGGTC GCCGTTTCTC AAATTTGGGC AGCATGCACA GCAGTATGGC TGGCTCTAAT 
ACATCTCTGT CCAGTGCGCA CAGCTTTAAT CGTGCCCAAG GAGGGGGCTC CTATACACCA 
ATGAGCTCCG GCTTGTCCAC ACCTGTCTCC CCGACGCCAG CTCACCTGCA GCACGTGCGG 
GAGCAGATGG CCGTTGCCCT CCGGAAGATC CGGGAGCTGG AAGAACAAGT GAAAACCATC 
CCAGTGCTGC AGGTCAAGAT CTCTGTACTT CAAGAGGAGA AGAGGCAGCT CAGTGTGCAA 
CTTAAGAGTC AGAAGTTCCT GGGACACACG CTGGGGTTCA ATCGAAGCCG TCCACGAGGC 
GAACTGTACA TCGACATACC TGAGGAGGAG GCAGGAAACG GGGCAGGAGC TGCAAACAAA 
GCCACAGGGA GTCTTTCACC CACCACTCCC GGCTCCCTCC AGGACTCAGG ATGTGAAATA 
GAGGAGACTG TAATTGTTGC GGGGGCTCGT CCGGGTGCAA AGCGGGAGGT GCGAACTGTA 
GGTGTGGGGC CTGAAGCAGA AGAAAGGGGT TATCGTCAAG TGGGGGTAGG GGTGCGAGAG 
CAGGACTTGG GTCTGCTGCC TGAAACCGAA GCCCTCAAGA CTAAAGTGGG TCTTTTAGAA 
GTTCAGCTGA GGAAAACCAT GCAAGAGCTA CAGAGTGCTC AGCAGCAAGT GGAAGCGGCA 
CAGAAAGAGA GGCAGACAGT TTGCCCTCAG GTAGACCATG CTGTGAGAGC CACCAGTCTC 
GGCTGGCAGG ATCAGCAGGG CCAGGCTGGA GCGGGCTTGC ATACTGTTGT CAGTTTTACT 
AAGCAACCTC ATCAGCAGAG GACTGTGGGC ATCCAAGTGT ACACGCTGGA GCAGCCCACA 
GTGGTGGGTG TGGGGACACT GCTAAGAGCC CAGGGCTGCA CTCATCCTGC TCAACTGGAG 
GCGTCTCACA GAAGATATGG AGAGTCCCCA GCAGCAGGGG ATTCGCCACA TGAGTTTCCC 
ATTGCAATAA GCTCTAAGCA GGTGCGGGAG GTCTTGAGAA GTGAGGTCTC CAAATCAGTG 
CCAGTGACCA ACCAGGCAAC TCCTATGGAG ACAAAGTGCA ACCAAGTGAC TGCGGTCTGC 
CAACGGCTAA AAGAAGGAGA GATTAACCAG CAACCAGCAG AAGAAGCCAT TCAAGTTGAT 
CCAGCCAATC CAGCATCCCC TCAGTCTAAC CTGAGGTCAA TCATGAAGAG GAAGGCTGAT 
GGGGAACCTG GCTCTCCATA CACCAAAAAG AACCTGCAGT TCTTAGGAGT CAATGGAGGG 
TACGAGTCAA CATCCTCCGA AGAGAGCAGC TCTGAAAGTT CAGAAGATGA AAGCGATGCA 
AGCGAATACC ATGAGGCTAC GGAAAAACTA CCGGAATCAG CGACACCACA GTCTCTGGTG 
TCCTCCTGTA TCCCACAGCT CGCCTCTGAG ACACCAGCTA CCCAAACAGC TCAGCACAGC 
ACTGCCCAGA TACCAACCAA TCACACTCCA GCAGCTCAAA CCACCAGCCA ATCACACACC 
ACAGATGCAA CTACCCAACA ACATGTCACC CAATCACCAG CAGCAGAGGC TGATGTACAG 
CACTGTGTCA GCCAATCATC AGTGACCACC ACACCTCCTC CAGAGGGAGA TGTTCAATGT 
GTTTTTCAAG GGTGTAACCC TCCAAGCACT GCCACTTCTC TTGAACAAAA CTCTGTCCAG 
TTGTCATCTG CGACGCAGCA GTCCAAAGCC ACTGATTCAA ACGTCCAGCC AGATCTCGCT 
CAAACAGATG TGTCGGACTT CGCCGCTCAG CAAACGACCA CTCAAACACA ATTCACCAGT 
GCCAAACCTC AGCAAGAAGC TAGCCAATCA AAAACTGCTG ACCTCACAGC CCAGAAAGGA 
GCGACACACT CTACTGATGG CTCAGCGAAA CAGGACATAG CCATATCCTC CACAACTAAA 
CCAGCAGCAG ATACAGCAAC ACCACCCACT AATAAACAAA CAGACAGCCT GGAATTAAGT 
GGCGGTCTTA TGTCGGCGCT CCACATCTTG CAGAAGGCGC TCAGCGAACC AAATGCATTC 
AGCCATCAAG ATGCGAGGAC GGCCTACACC AGCGTCCTGC AGGAGTGGCT CCGGGTCTCC 
TGTCATAAAG CTGCTGACAC CGCAGTGGTG AAGGCCCACA TGGACGCGTT CGCTTCTATT 
TCCCCTCAGC TCCTGGAGTT CGTCATCAAC ATGGCCGACG GCAATGGAAA CACGGCTCTG 
CACTACACCG TCTCTCACTC CAACTTCCCT GTAGTGAAGC TGCTGCTGGA CACCGGTCTC 
TGTAATGCTG ACAAGCAGAA CAAGGCTGGT TACACAGCCA TCATGTTGAC GGCTCTGGCT 
GCCTTCAGCT CGGACAGTGA CCTTCAGACC GTCCTACAGC TGCTCCGCAC CGGCGACGTC 
AATGCCAAAG CCAGCCAGGC AGGGCAGACG GCACTGATGC TGGCAGTCAG TCATGGTCGA 
GGGGATATGG TGAAGGCTCT GCTGGCATGT GGAGCTCAGG TGAACCTGCG GGATGACGAC 
GGCTCCACAG CGCTCATGTG CGCCTGTGAA CATGGACATG TGGACATCGT GCGCCAGCTG 
CTGTCTGTGC CCGGCTGTGA CGCCACGCTC ACTGATAATG ATGGCAGCAC TGCTCTCTCC 
ATCGCGTTGG AGGCCAGTCA GAATGACATT GCTGTGCTTC TATATGCACA CCTCAATTTT 
GCCAAGCCGC CTTCTCCTGT ATCACCAAAG TCTCCAATTC TGGGCTCTTC TCCTCCTTCG 
TCCTCTGAAC TGAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kank2 ( XM_005164339.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005164396.1 Danio rerio KN motif and ankyrin repeat domains 2 (kank2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa08860
Clone ID Related Accession (Same CDS sequence)	XM_005164339.3 , XM_005164339.4 , XM_021471168.1 , NM_001305620.1
Accession Version	XM_005164339.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3258bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	si:ch73-108m21.1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005164339.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACAATCA TGGCTCAGGT GCTTCATATG GACTCCAGTT TTCCTGGGAA GATTAACCCC 
CCGGTGCCCC CCTCCTTGCA TGCAAAGGAC CAAGAGGCTC CATATTCAGT AGAAACTCCT 
TACGGATACC GTCTGGACCT TGACTTTCTC AAGTATGTCA ATGATATTGA GAAAGGAAAC 
ACTATTAAGA AGGTGCCAGT GCAGCGCCGC CCACGTTATG GCTCGCTTCC ACGTGGCTAC 
GGCTACACAG GCTCCTGGTG GACCTCCACA GAGTCCCTGT GCTCCAATGC CAGCATGGAC 
AGCCGGCACT CATCATATTC TTACTGTGCA CCAGGCTTCC ACACCTCGCA GAGACCCAAC 
TTCAGTACAG CACGTGTAGA GAAAACCCTA ATGGATGCTC GCCGGAAGCT GGAGGAAGAG 
AAAGATGGTC GCCGTTTCTC AAATTTGGGC AGCATGCACA GCAGTATGGC TGGCTCTAAT 
ACATCTCTGT CCAGTGCGCA CAGCTTTAAT CGTGCCCAAG GAGGGGGCTC CTATACACCA 
ATGAGCTCCG GCTTGTCCAC ACCTGTCTCC CCGACGCCAG CTCACCTGCA GCACGTGCGG 
GAGCAGATGG CCGTTGCCCT CCGGAAGATC CGGGAGCTGG AAGAACAAGT GAAAACCATC 
CCAGTGCTGC AGGTCAAGAT CTCTGTACTT CAAGAGGAGA AGAGGCAGCT CAGTGTGCAA 
CTTAAGAGTC AGAAGTTCCT GGGACACACG CTGGGGTTCA ATCGAAGCCG TCCACGAGGC 
GAACTGTACA TCGACATACC TGAGGAGGAG GCAGGAAACG GGGCAGGAGC TGCAAACAAA 
GCCACAGGGA GTCTTTCACC CACCACTCCC GGCTCCCTCC AGGACTCAGG ATGTGAAATA 
GAGGAGACTG TAATTGTTGC GGGGGCTCGT CCGGGTGCAA AGCGGGAGGT GCGAACTGTA 
GGTGTGGGGC CTGAAGCAGA AGAAAGGGGT TATCGTCAAG TGGGGGTAGG GGTGCGAGAG 
CAGGACTTGG GTCTGCTGCC TGAAACCGAA GCCCTCAAGA CTAAAGTGGG TCTTTTAGAA 
GTTCAGCTGA GGAAAACCAT GCAAGAGCTA CAGAGTGCTC AGCAGCAAGT GGAAGCGGCA 
CAGAAAGAGA GGCAGACAGT TTGCCCTCAG GTAGACCATG CTGTGAGAGC CACCAGTCTC 
GGCTGGCAGG ATCAGCAGGG CCAGGCTGGA GCGGGCTTGC ATACTGTTGT CAGTTTTACT 
AAGCAACCTC ATCAGCAGAG GACTGTGGGC ATCCAAGTGT ACACGCTGGA GCAGCCCACA 
GTGGTGGGTG TGGGGACACT GCTAAGAGCC CAGGGCTGCA CTCATCCTGC TCAACTGGAG 
GCGTCTCACA GAAGATATGG AGAGTCCCCA GCAGCAGGGG ATTCGCCACA TGAGTTTCCC 
ATTGCAATAA GCTCTAAGCA GGTGCGGGAG GTCTTGAGAA GTGAGGTCTC CAAATCAGTG 
CCAGTGACCA ACCAGGCAAC TCCTATGGAG ACAAAGTGCA ACCAAGTGAC TGCGGTCTGC 
CAACGGCTAA AAGAAGGAGA GATTAACCAG CAACCAGCAG AAGAAGCCAT TCAAGTTGAT 
CCAGCCAATC CAGCATCCCC TCAGTCTAAC CTGAGGTCAA TCATGAAGAG GAAGGCTGAT 
GGGGAACCTG GCTCTCCATA CACCAAAAAG AACCTGCAGT TCTTAGGAGT CAATGGAGGG 
TACGAGTCAA CATCCTCCGA AGAGAGCAGC TCTGAAAGTT CAGAAGATGA AAGCGATGCA 
AGCGAATACC ATGAGGCTAC GGAAAAACTA CCGGAATCAG CGACACCACA GTCTCTGGTG 
TCCTCCTGTA TCCCACAGCT CGCCTCTGAG ACACCAGCTA CCCAAACAGC TCAGCACAGC 
ACTGCCCAGA TACCAACCAA TCACACTCCA GCAGCTCAAA CCACCAGCCA ATCACACACC 
ACAGATGCAA CTACCCAACA ACATGTCACC CAATCACCAG CAGCAGAGGC TGATGTACAG 
CACTGTGTCA GCCAATCATC AGTGACCACC ACACCTCCTC CAGAGGGAGA TGTTCAATGT 
GTTTTTCAAG GGTGTAACCC TCCAAGCACT GCCACTTCTC TTGAACAAAA CTCTGTCCAG 
TTGTCATCTG CGACGCAGCA GTCCAAAGCC ACTGATTCAA ACGTCCAGCC AGATCTCGCT 
CAAACAGATG TGTCGGACTT CGCCGCTCAG CAAACGACCA CTCAAACACA ATTCACCAGT 
GCCAAACCTC AGCAAGAAGC TAGCCAATCA AAAACTGCTG ACCTCACAGC CCAGAAAGGA 
GCGACACACT CTACTGATGG CTCAGCGAAA CAGGACATAG CCATATCCTC CACAACTAAA 
CCAGCAGCAG ATACAGCAAC ACCACCCACT AATAAACAAA CAGACAGCCT GGAATTAAGT 
GGCGGTCTTA TGTCGGCGCT CCACATCTTG CAGAAGGCGC TCAGCGAACC AAATGCATTC 
AGCCATCAAG ATGCGAGGAC GGCCTACACC AGCGTCCTGC AGGAGTGGCT CCGGGTCTCC 
TGTCATAAAG CTGCTGACAC CGCAGTGGTG AAGGCCCACA TGGACGCGTT CGCTTCTATT 
TCCCCTCAGC TCCTGGAGTT CGTCATCAAC ATGGCCGACG GCAATGGAAA CACGGCTCTG 
CACTACACCG TCTCTCACTC CAACTTCCCT GTAGTGAAGC TGCTGCTGGA CACCGGTCTC 
TGTAATGCTG ACAAGCAGAA CAAGGCTGGT TACACAGCCA TCATGTTGAC GGCTCTGGCT 
GCCTTCAGCT CGGACAGTGA CCTTCAGACC GTCCTACAGC TGCTCCGCAC CGGCGACGTC 
AATGCCAAAG CCAGCCAGGC AGGGCAGACG GCACTGATGC TGGCAGTCAG TCATGGTCGA 
GGGGATATGG TGAAGGCTCT GCTGGCATGT GGAGCTCAGG TGAACCTGCG GGATGACGAC 
GGCTCCACAG CGCTCATGTG CGCCTGTGAA CATGGACATG TGGACATCGT GCGCCAGCTG 
CTGTCTGTGC CCGGCTGTGA CGCCACGCTC ACTGATAATG ATGGCAGCAC TGCTCTCTCC 
ATCGCGTTGG AGGCCAGTCA GAATGACATT GCTGTGCTTC TATATGCACA CCTCAATTTT 
GCCAAGCCGC CTTCTCCTGT ATCACCAAAG TCTCCAATTC TGGGCTCTTC TCCTCCTTCG 
TCCTCTGAAC TGAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005164396.1
CDS	249..3506
Translation	MTIMAQVLHMDSSFPGKINPPVPPSLHAKDQEAPYSVETPYGYRLDLDFLKYVNDIEKGNTIKKVPVQRRPRYGSLPRGYGYTGSWWTSTESLCSNASMDSRHSSYSYCAPGFHTSQRPNFSTARVEKTLMDARRKLEEEKDGRRFSNLGSMHSSMAGSNTSLSSAHSFNRAQGGGSYTPMSSGLSTPVSPTPAHLQHVREQMAVALRKIRELEEQVKTIPVLQVKISVLQEEKRQLSVQLKSQKFLGHTLGFNRSRPRGELYIDIPEEEAGNGAGAANKATGSLSPTTPGSLQDSGCEIEETVIVAGARPGAKREVRTVGVGPEAEERGYRQVGVGVREQDLGLLPETEALKTKVGLLEVQLRKTMQELQSAQQQVEAAQKERQTVCPQVDHAVRATSLGWQDQQGQAGAGLHTVVSFTKQPHQQRTVGIQVYTLEQPTVVGVGTLLRAQGCTHPAQLEASHRRYGESPAAGDSPHEFPIAISSKQVREVLRSEVSKSVPVTNQATPMETKCNQVTAVCQRLKEGEINQQPAEEAIQVDPANPASPQSNLRSIMKRKADGEPGSPYTKKNLQFLGVNGGYESTSSEESSSESSEDESDASEYHEATEKLPESATPQSLVSSCIPQLASETPATQTAQHSTAQIPTNHTPAAQTTSQSHTTDATTQQHVTQSPAAEADVQHCVSQSSVTTTPPPEGDVQCVFQGCNPPSTATSLEQNSVQLSSATQQSKATDSNVQPDLAQTDVSDFAAQQTTTQTQFTSAKPQQEASQSKTADLTAQKGATHSTDGSAKQDIAISSTTKPAADTATPPTNKQTDSLELSGGLMSALHILQKALSEPNAFSHQDARTAYTSVLQEWLRVSCHKAADTAVVKAHMDAFASISPQLLEFVINMADGNGNTALHYTVSHSNFPVVKLLLDTGLCNADKQNKAGYTAIMLTALAAFSSDSDLQTVLQLLRTGDVNAKASQAGQTALMLAVSHGRGDMVKALLACGAQVNLRDDDGSTALMCACEHGHVDIVRQLLSVPGCDATLTDNDGSTALSIALEASQNDIAVLLYAHLNFAKPPSPVSPKSPILGSSPPSSSELK

Target ORF information:

RefSeq Version	XM_005164339.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio KN motif and ankyrin repeat domains 2 (kank2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005164339.4

ORF Insert Sequence:

ATGACAATCA TGGCTCAGGT GCTTCATATG GACTCCAGTT TTCCTGGGAA GATTAACCCC 
CCGGTGCCCC CCTCCTTGCA TGCAAAGGAC CAAGAGGCTC CATATTCAGT AGAAACTCCT 
TACGGATACC GTCTGGACCT TGACTTTCTC AAGTATGTCA ATGATATTGA GAAAGGAAAC 
ACTATTAAGA AGGTGCCAGT GCAGCGCCGC CCACGTTATG GCTCGCTTCC ACGTGGCTAC 
GGCTACACAG GCTCCTGGTG GACCTCCACA GAGTCCCTGT GCTCCAATGC CAGCATGGAC 
AGCCGGCACT CATCATATTC TTACTGTGCA CCAGGCTTCC ACACCTCGCA GAGACCCAAC 
TTCAGTACAG CACGTGTAGA GAAAACCCTA ATGGATGCTC GCCGGAAGCT GGAGGAAGAG 
AAAGATGGTC GCCGTTTCTC AAATTTGGGC AGCATGCACA GCAGTATGGC TGGCTCTAAT 
ACATCTCTGT CCAGTGCGCA CAGCTTTAAT CGTGCCCAAG GAGGGGGCTC CTATACACCA 
ATGAGCTCCG GCTTGTCCAC ACCTGTCTCC CCGACGCCAG CTCACCTGCA GCACGTGCGG 
GAGCAGATGG CCGTTGCCCT CCGGAAGATC CGGGAGCTGG AAGAACAAGT GAAAACCATC 
CCAGTGCTGC AGGTCAAGAT CTCTGTACTT CAAGAGGAGA AGAGGCAGCT CAGTGTGCAA 
CTTAAGAGTC AGAAGTTCCT GGGACACACG CTGGGGTTCA ATCGAAGCCG TCCACGAGGC 
GAACTGTACA TCGACATACC TGAGGAGGAG GCAGGAAACG GGGCAGGAGC TGCAAACAAA 
GCCACAGGGA GTCTTTCACC CACCACTCCC GGCTCCCTCC AGGACTCAGG ATGTGAAATA 
GAGGAGACTG TAATTGTTGC GGGGGCTCGT CCGGGTGCAA AGCGGGAGGT GCGAACTGTA 
GGTGTGGGGC CTGAAGCAGA AGAAAGGGGT TATCGTCAAG TGGGGGTAGG GGTGCGAGAG 
CAGGACTTGG GTCTGCTGCC TGAAACCGAA GCCCTCAAGA CTAAAGTGGG TCTTTTAGAA 
GTTCAGCTGA GGAAAACCAT GCAAGAGCTA CAGAGTGCTC AGCAGCAAGT GGAAGCGGCA 
CAGAAAGAGA GGCAGACAGT TTGCCCTCAG GTAGACCATG CTGTGAGAGC CACCAGTCTC 
GGCTGGCAGG ATCAGCAGGG CCAGGCTGGA GCGGGCTTGC ATACTGTTGT CAGTTTTACT 
AAGCAACCTC ATCAGCAGAG GACTGTGGGC ATCCAAGTGT ACACGCTGGA GCAGCCCACA 
GTGGTGGGTG TGGGGACACT GCTAAGAGCC CAGGGCTGCA CTCATCCTGC TCAACTGGAG 
GCGTCTCACA GAAGATATGG AGAGTCCCCA GCAGCAGGGG ATTCGCCACA TGAGTTTCCC 
ATTGCAATAA GCTCTAAGCA GGTGCGGGAG GTCTTGAGAA GTGAGGTCTC CAAATCAGTG 
CCAGTGACCA ACCAGGCAAC TCCTATGGAG ACAAAGTGCA ACCAAGTGAC TGCGGTCTGC 
CAACGGCTAA AAGAAGGAGA GATTAACCAG CAACCAGCAG AAGAAGCCAT TCAAGTTGAT 
CCAGCCAATC CAGCATCCCC TCAGTCTAAC CTGAGGTCAA TCATGAAGAG GAAGGCTGAT 
GGGGAACCTG GCTCTCCATA CACCAAAAAG AACCTGCAGT TCTTAGGAGT CAATGGAGGG 
TACGAGTCAA CATCCTCCGA AGAGAGCAGC TCTGAAAGTT CAGAAGATGA AAGCGATGCA 
AGCGAATACC ATGAGGCTAC GGAAAAACTA CCGGAATCAG CGACACCACA GTCTCTGGTG 
TCCTCCTGTA TCCCACAGCT CGCCTCTGAG ACACCAGCTA CCCAAACAGC TCAGCACAGC 
ACTGCCCAGA TACCAACCAA TCACACTCCA GCAGCTCAAA CCACCAGCCA ATCACACACC 
ACAGATGCAA CTACCCAACA ACATGTCACC CAATCACCAG CAGCAGAGGC TGATGTACAG 
CACTGTGTCA GCCAATCATC AGTGACCACC ACACCTCCTC CAGAGGGAGA TGTTCAATGT 
GTTTTTCAAG GGTGTAACCC TCCAAGCACT GCCACTTCTC TTGAACAAAA CTCTGTCCAG 
TTGTCATCTG CGACGCAGCA GTCCAAAGCC ACTGATTCAA ACGTCCAGCC AGATCTCGCT 
CAAACAGATG TGTCGGACTT CGCCGCTCAG CAAACGACCA CTCAAACACA ATTCACCAGT 
GCCAAACCTC AGCAAGAAGC TAGCCAATCA AAAACTGCTG ACCTCACAGC CCAGAAAGGA 
GCGACACACT CTACTGATGG CTCAGCGAAA CAGGACATAG CCATATCCTC CACAACTAAA 
CCAGCAGCAG ATACAGCAAC ACCACCCACT AATAAACAAA CAGACAGCCT GGAATTAAGT 
GGCGGTCTTA TGTCGGCGCT CCACATCTTG CAGAAGGCGC TCAGCGAACC AAATGCATTC 
AGCCATCAAG ATGCGAGGAC GGCCTACACC AGCGTCCTGC AGGAGTGGCT CCGGGTCTCC 
TGTCATAAAG CTGCTGACAC CGCAGTGGTG AAGGCCCACA TGGACGCGTT CGCTTCTATT 
TCCCCTCAGC TCCTGGAGTT CGTCATCAAC ATGGCCGACG GCAATGGAAA CACGGCTCTG 
CACTACACCG TCTCTCACTC CAACTTCCCT GTAGTGAAGC TGCTGCTGGA CACCGGTCTC 
TGTAATGCTG ACAAGCAGAA CAAGGCTGGT TACACAGCCA TCATGTTGAC GGCTCTGGCT 
GCCTTCAGCT CGGACAGTGA CCTTCAGACC GTCCTACAGC TGCTCCGCAC CGGCGACGTC 
AATGCCAAAG CCAGCCAGGC AGGGCAGACG GCACTGATGC TGGCAGTCAG TCATGGTCGA 
GGGGATATGG TGAAGGCTCT GCTGGCATGT GGAGCTCAGG TGAACCTGCG GGATGACGAC 
GGCTCCACAG CGCTCATGTG CGCCTGTGAA CATGGACATG TGGACATCGT GCGCCAGCTG 
CTGTCTGTGC CCGGCTGTGA CGCCACGCTC ACTGATAATG ATGGCAGCAC TGCTCTCTCC 
ATCGCGTTGG AGGCCAGTCA GAATGACATT GCTGTGCTTC TATATGCACA CCTCAATTTT 
GCCAAGCCGC CTTCTCCTGT ATCACCAAAG TCTCCAATTC TGGGCTCTTC TCCTCCTTCG 
TCCTCTGAAC TGAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kank2 ( XM_021471168.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021326843.1 Danio rerio KN motif and ankyrin repeat domains 2 (kank2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa08860
Clone ID Related Accession (Same CDS sequence)	XM_005164339.3 , XM_005164339.4 , XM_021471168.1 , NM_001305620.1
Accession Version	XM_021471168.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3258bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	si:ch73-108m21.1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACAATCA TGGCTCAGGT GCTTCATATG GACTCCAGTT TTCCTGGGAA GATTAACCCC 
CCGGTGCCCC CCTCCTTGCA TGCAAAGGAC CAAGAGGCTC CATATTCAGT AGAAACTCCT 
TACGGATACC GTCTGGACCT TGACTTTCTC AAGTATGTCA ATGATATTGA GAAAGGAAAC 
ACTATTAAGA AGGTGCCAGT GCAGCGCCGC CCACGTTATG GCTCGCTTCC ACGTGGCTAC 
GGCTACACAG GCTCCTGGTG GACCTCCACA GAGTCCCTGT GCTCCAATGC CAGCATGGAC 
AGCCGGCACT CATCATATTC TTACTGTGCA CCAGGCTTCC ACACCTCGCA GAGACCCAAC 
TTCAGTACAG CACGTGTAGA GAAAACCCTA ATGGATGCTC GCCGGAAGCT GGAGGAAGAG 
AAAGATGGTC GCCGTTTCTC AAATTTGGGC AGCATGCACA GCAGTATGGC TGGCTCTAAT 
ACATCTCTGT CCAGTGCGCA CAGCTTTAAT CGTGCCCAAG GAGGGGGCTC CTATACACCA 
ATGAGCTCCG GCTTGTCCAC ACCTGTCTCC CCGACGCCAG CTCACCTGCA GCACGTGCGG 
GAGCAGATGG CCGTTGCCCT CCGGAAGATC CGGGAGCTGG AAGAACAAGT GAAAACCATC 
CCAGTGCTGC AGGTCAAGAT CTCTGTACTT CAAGAGGAGA AGAGGCAGCT CAGTGTGCAA 
CTTAAGAGTC AGAAGTTCCT GGGACACACG CTGGGGTTCA ATCGAAGCCG TCCACGAGGC 
GAACTGTACA TCGACATACC TGAGGAGGAG GCAGGAAACG GGGCAGGAGC TGCAAACAAA 
GCCACAGGGA GTCTTTCACC CACCACTCCC GGCTCCCTCC AGGACTCAGG ATGTGAAATA 
GAGGAGACTG TAATTGTTGC GGGGGCTCGT CCGGGTGCAA AGCGGGAGGT GCGAACTGTA 
GGTGTGGGGC CTGAAGCAGA AGAAAGGGGT TATCGTCAAG TGGGGGTAGG GGTGCGAGAG 
CAGGACTTGG GTCTGCTGCC TGAAACCGAA GCCCTCAAGA CTAAAGTGGG TCTTTTAGAA 
GTTCAGCTGA GGAAAACCAT GCAAGAGCTA CAGAGTGCTC AGCAGCAAGT GGAAGCGGCA 
CAGAAAGAGA GGCAGACAGT TTGCCCTCAG GTAGACCATG CTGTGAGAGC CACCAGTCTC 
GGCTGGCAGG ATCAGCAGGG CCAGGCTGGA GCGGGCTTGC ATACTGTTGT CAGTTTTACT 
AAGCAACCTC ATCAGCAGAG GACTGTGGGC ATCCAAGTGT ACACGCTGGA GCAGCCCACA 
GTGGTGGGTG TGGGGACACT GCTAAGAGCC CAGGGCTGCA CTCATCCTGC TCAACTGGAG 
GCGTCTCACA GAAGATATGG AGAGTCCCCA GCAGCAGGGG ATTCGCCACA TGAGTTTCCC 
ATTGCAATAA GCTCTAAGCA GGTGCGGGAG GTCTTGAGAA GTGAGGTCTC CAAATCAGTG 
CCAGTGACCA ACCAGGCAAC TCCTATGGAG ACAAAGTGCA ACCAAGTGAC TGCGGTCTGC 
CAACGGCTAA AAGAAGGAGA GATTAACCAG CAACCAGCAG AAGAAGCCAT TCAAGTTGAT 
CCAGCCAATC CAGCATCCCC TCAGTCTAAC CTGAGGTCAA TCATGAAGAG GAAGGCTGAT 
GGGGAACCTG GCTCTCCATA CACCAAAAAG AACCTGCAGT TCTTAGGAGT CAATGGAGGG 
TACGAGTCAA CATCCTCCGA AGAGAGCAGC TCTGAAAGTT CAGAAGATGA AAGCGATGCA 
AGCGAATACC ATGAGGCTAC GGAAAAACTA CCGGAATCAG CGACACCACA GTCTCTGGTG 
TCCTCCTGTA TCCCACAGCT CGCCTCTGAG ACACCAGCTA CCCAAACAGC TCAGCACAGC 
ACTGCCCAGA TACCAACCAA TCACACTCCA GCAGCTCAAA CCACCAGCCA ATCACACACC 
ACAGATGCAA CTACCCAACA ACATGTCACC CAATCACCAG CAGCAGAGGC TGATGTACAG 
CACTGTGTCA GCCAATCATC AGTGACCACC ACACCTCCTC CAGAGGGAGA TGTTCAATGT 
GTTTTTCAAG GGTGTAACCC TCCAAGCACT GCCACTTCTC TTGAACAAAA CTCTGTCCAG 
TTGTCATCTG CGACGCAGCA GTCCAAAGCC ACTGATTCAA ACGTCCAGCC AGATCTCGCT 
CAAACAGATG TGTCGGACTT CGCCGCTCAG CAAACGACCA CTCAAACACA ATTCACCAGT 
GCCAAACCTC AGCAAGAAGC TAGCCAATCA AAAACTGCTG ACCTCACAGC CCAGAAAGGA 
GCGACACACT CTACTGATGG CTCAGCGAAA CAGGACATAG CCATATCCTC CACAACTAAA 
CCAGCAGCAG ATACAGCAAC ACCACCCACT AATAAACAAA CAGACAGCCT GGAATTAAGT 
GGCGGTCTTA TGTCGGCGCT CCACATCTTG CAGAAGGCGC TCAGCGAACC AAATGCATTC 
AGCCATCAAG ATGCGAGGAC GGCCTACACC AGCGTCCTGC AGGAGTGGCT CCGGGTCTCC 
TGTCATAAAG CTGCTGACAC CGCAGTGGTG AAGGCCCACA TGGACGCGTT CGCTTCTATT 
TCCCCTCAGC TCCTGGAGTT CGTCATCAAC ATGGCCGACG GCAATGGAAA CACGGCTCTG 
CACTACACCG TCTCTCACTC CAACTTCCCT GTAGTGAAGC TGCTGCTGGA CACCGGTCTC 
TGTAATGCTG ACAAGCAGAA CAAGGCTGGT TACACAGCCA TCATGTTGAC GGCTCTGGCT 
GCCTTCAGCT CGGACAGTGA CCTTCAGACC GTCCTACAGC TGCTCCGCAC CGGCGACGTC 
AATGCCAAAG CCAGCCAGGC AGGGCAGACG GCACTGATGC TGGCAGTCAG TCATGGTCGA 
GGGGATATGG TGAAGGCTCT GCTGGCATGT GGAGCTCAGG TGAACCTGCG GGATGACGAC 
GGCTCCACAG CGCTCATGTG CGCCTGTGAA CATGGACATG TGGACATCGT GCGCCAGCTG 
CTGTCTGTGC CCGGCTGTGA CGCCACGCTC ACTGATAATG ATGGCAGCAC TGCTCTCTCC 
ATCGCGTTGG AGGCCAGTCA GAATGACATT GCTGTGCTTC TATATGCACA CCTCAATTTT 
GCCAAGCCGC CTTCTCCTGT ATCACCAAAG TCTCCAATTC TGGGCTCTTC TCCTCCTTCG 
TCCTCTGAAC TGAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021326843.1
CDS	294..3551
Translation	MTIMAQVLHMDSSFPGKINPPVPPSLHAKDQEAPYSVETPYGYRLDLDFLKYVNDIEKGNTIKKVPVQRRPRYGSLPRGYGYTGSWWTSTESLCSNASMDSRHSSYSYCAPGFHTSQRPNFSTARVEKTLMDARRKLEEEKDGRRFSNLGSMHSSMAGSNTSLSSAHSFNRAQGGGSYTPMSSGLSTPVSPTPAHLQHVREQMAVALRKIRELEEQVKTIPVLQVKISVLQEEKRQLSVQLKSQKFLGHTLGFNRSRPRGELYIDIPEEEAGNGAGAANKATGSLSPTTPGSLQDSGCEIEETVIVAGARPGAKREVRTVGVGPEAEERGYRQVGVGVREQDLGLLPETEALKTKVGLLEVQLRKTMQELQSAQQQVEAAQKERQTVCPQVDHAVRATSLGWQDQQGQAGAGLHTVVSFTKQPHQQRTVGIQVYTLEQPTVVGVGTLLRAQGCTHPAQLEASHRRYGESPAAGDSPHEFPIAISSKQVREVLRSEVSKSVPVTNQATPMETKCNQVTAVCQRLKEGEINQQPAEEAIQVDPANPASPQSNLRSIMKRKADGEPGSPYTKKNLQFLGVNGGYESTSSEESSSESSEDESDASEYHEATEKLPESATPQSLVSSCIPQLASETPATQTAQHSTAQIPTNHTPAAQTTSQSHTTDATTQQHVTQSPAAEADVQHCVSQSSVTTTPPPEGDVQCVFQGCNPPSTATSLEQNSVQLSSATQQSKATDSNVQPDLAQTDVSDFAAQQTTTQTQFTSAKPQQEASQSKTADLTAQKGATHSTDGSAKQDIAISSTTKPAADTATPPTNKQTDSLELSGGLMSALHILQKALSEPNAFSHQDARTAYTSVLQEWLRVSCHKAADTAVVKAHMDAFASISPQLLEFVINMADGNGNTALHYTVSHSNFPVVKLLLDTGLCNADKQNKAGYTAIMLTALAAFSSDSDLQTVLQLLRTGDVNAKASQAGQTALMLAVSHGRGDMVKALLACGAQVNLRDDDGSTALMCACEHGHVDIVRQLLSVPGCDATLTDNDGSTALSIALEASQNDIAVLLYAHLNFAKPPSPVSPKSPILGSSPPSSSELK

Target ORF information:

RefSeq Version	XM_021471168.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio KN motif and ankyrin repeat domains 2 (kank2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021471168.1

ORF Insert Sequence:

ATGACAATCA TGGCTCAGGT GCTTCATATG GACTCCAGTT TTCCTGGGAA GATTAACCCC 
CCGGTGCCCC CCTCCTTGCA TGCAAAGGAC CAAGAGGCTC CATATTCAGT AGAAACTCCT 
TACGGATACC GTCTGGACCT TGACTTTCTC AAGTATGTCA ATGATATTGA GAAAGGAAAC 
ACTATTAAGA AGGTGCCAGT GCAGCGCCGC CCACGTTATG GCTCGCTTCC ACGTGGCTAC 
GGCTACACAG GCTCCTGGTG GACCTCCACA GAGTCCCTGT GCTCCAATGC CAGCATGGAC 
AGCCGGCACT CATCATATTC TTACTGTGCA CCAGGCTTCC ACACCTCGCA GAGACCCAAC 
TTCAGTACAG CACGTGTAGA GAAAACCCTA ATGGATGCTC GCCGGAAGCT GGAGGAAGAG 
AAAGATGGTC GCCGTTTCTC AAATTTGGGC AGCATGCACA GCAGTATGGC TGGCTCTAAT 
ACATCTCTGT CCAGTGCGCA CAGCTTTAAT CGTGCCCAAG GAGGGGGCTC CTATACACCA 
ATGAGCTCCG GCTTGTCCAC ACCTGTCTCC CCGACGCCAG CTCACCTGCA GCACGTGCGG 
GAGCAGATGG CCGTTGCCCT CCGGAAGATC CGGGAGCTGG AAGAACAAGT GAAAACCATC 
CCAGTGCTGC AGGTCAAGAT CTCTGTACTT CAAGAGGAGA AGAGGCAGCT CAGTGTGCAA 
CTTAAGAGTC AGAAGTTCCT GGGACACACG CTGGGGTTCA ATCGAAGCCG TCCACGAGGC 
GAACTGTACA TCGACATACC TGAGGAGGAG GCAGGAAACG GGGCAGGAGC TGCAAACAAA 
GCCACAGGGA GTCTTTCACC CACCACTCCC GGCTCCCTCC AGGACTCAGG ATGTGAAATA 
GAGGAGACTG TAATTGTTGC GGGGGCTCGT CCGGGTGCAA AGCGGGAGGT GCGAACTGTA 
GGTGTGGGGC CTGAAGCAGA AGAAAGGGGT TATCGTCAAG TGGGGGTAGG GGTGCGAGAG 
CAGGACTTGG GTCTGCTGCC TGAAACCGAA GCCCTCAAGA CTAAAGTGGG TCTTTTAGAA 
GTTCAGCTGA GGAAAACCAT GCAAGAGCTA CAGAGTGCTC AGCAGCAAGT GGAAGCGGCA 
CAGAAAGAGA GGCAGACAGT TTGCCCTCAG GTAGACCATG CTGTGAGAGC CACCAGTCTC 
GGCTGGCAGG ATCAGCAGGG CCAGGCTGGA GCGGGCTTGC ATACTGTTGT CAGTTTTACT 
AAGCAACCTC ATCAGCAGAG GACTGTGGGC ATCCAAGTGT ACACGCTGGA GCAGCCCACA 
GTGGTGGGTG TGGGGACACT GCTAAGAGCC CAGGGCTGCA CTCATCCTGC TCAACTGGAG 
GCGTCTCACA GAAGATATGG AGAGTCCCCA GCAGCAGGGG ATTCGCCACA TGAGTTTCCC 
ATTGCAATAA GCTCTAAGCA GGTGCGGGAG GTCTTGAGAA GTGAGGTCTC CAAATCAGTG 
CCAGTGACCA ACCAGGCAAC TCCTATGGAG ACAAAGTGCA ACCAAGTGAC TGCGGTCTGC 
CAACGGCTAA AAGAAGGAGA GATTAACCAG CAACCAGCAG AAGAAGCCAT TCAAGTTGAT 
CCAGCCAATC CAGCATCCCC TCAGTCTAAC CTGAGGTCAA TCATGAAGAG GAAGGCTGAT 
GGGGAACCTG GCTCTCCATA CACCAAAAAG AACCTGCAGT TCTTAGGAGT CAATGGAGGG 
TACGAGTCAA CATCCTCCGA AGAGAGCAGC TCTGAAAGTT CAGAAGATGA AAGCGATGCA 
AGCGAATACC ATGAGGCTAC GGAAAAACTA CCGGAATCAG CGACACCACA GTCTCTGGTG 
TCCTCCTGTA TCCCACAGCT CGCCTCTGAG ACACCAGCTA CCCAAACAGC TCAGCACAGC 
ACTGCCCAGA TACCAACCAA TCACACTCCA GCAGCTCAAA CCACCAGCCA ATCACACACC 
ACAGATGCAA CTACCCAACA ACATGTCACC CAATCACCAG CAGCAGAGGC TGATGTACAG 
CACTGTGTCA GCCAATCATC AGTGACCACC ACACCTCCTC CAGAGGGAGA TGTTCAATGT 
GTTTTTCAAG GGTGTAACCC TCCAAGCACT GCCACTTCTC TTGAACAAAA CTCTGTCCAG 
TTGTCATCTG CGACGCAGCA GTCCAAAGCC ACTGATTCAA ACGTCCAGCC AGATCTCGCT 
CAAACAGATG TGTCGGACTT CGCCGCTCAG CAAACGACCA CTCAAACACA ATTCACCAGT 
GCCAAACCTC AGCAAGAAGC TAGCCAATCA AAAACTGCTG ACCTCACAGC CCAGAAAGGA 
GCGACACACT CTACTGATGG CTCAGCGAAA CAGGACATAG CCATATCCTC CACAACTAAA 
CCAGCAGCAG ATACAGCAAC ACCACCCACT AATAAACAAA CAGACAGCCT GGAATTAAGT 
GGCGGTCTTA TGTCGGCGCT CCACATCTTG CAGAAGGCGC TCAGCGAACC AAATGCATTC 
AGCCATCAAG ATGCGAGGAC GGCCTACACC AGCGTCCTGC AGGAGTGGCT CCGGGTCTCC 
TGTCATAAAG CTGCTGACAC CGCAGTGGTG AAGGCCCACA TGGACGCGTT CGCTTCTATT 
TCCCCTCAGC TCCTGGAGTT CGTCATCAAC ATGGCCGACG GCAATGGAAA CACGGCTCTG 
CACTACACCG TCTCTCACTC CAACTTCCCT GTAGTGAAGC TGCTGCTGGA CACCGGTCTC 
TGTAATGCTG ACAAGCAGAA CAAGGCTGGT TACACAGCCA TCATGTTGAC GGCTCTGGCT 
GCCTTCAGCT CGGACAGTGA CCTTCAGACC GTCCTACAGC TGCTCCGCAC CGGCGACGTC 
AATGCCAAAG CCAGCCAGGC AGGGCAGACG GCACTGATGC TGGCAGTCAG TCATGGTCGA 
GGGGATATGG TGAAGGCTCT GCTGGCATGT GGAGCTCAGG TGAACCTGCG GGATGACGAC 
GGCTCCACAG CGCTCATGTG CGCCTGTGAA CATGGACATG TGGACATCGT GCGCCAGCTG 
CTGTCTGTGC CCGGCTGTGA CGCCACGCTC ACTGATAATG ATGGCAGCAC TGCTCTCTCC 
ATCGCGTTGG AGGCCAGTCA GAATGACATT GCTGTGCTTC TATATGCACA CCTCAATTTT 
GCCAAGCCGC CTTCTCCTGT ATCACCAAAG TCTCCAATTC TGGGCTCTTC TCCTCCTTCG 
TCCTCTGAAC TGAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Evolutionary and developmental analysis reveals KANK genes were co-opted for vertebrate vascular development.
Scientific reports627816(2016 Jun)
Hensley MR,Cui Z,Chua RF,Simpson S,Shammas NL,Yang JY,Leung YF,Zhang G

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

KANK deficiency leads to podocyte dysfunction and nephrotic syndrome.
The Journal of clinical investigation125(6)2375-84(2015 Jun)
Gee HY,Zhang F,Ashraf S,Kohl S,Sadowski CE,Vega-Warner V,Zhou W,Lovric S,Fang H,Nettleton M,Zhu JY,Hoefele J,Weber LT,Podracka L,Boor A,Fehrenbach H,Innis JW,Washburn J,Levy S,Lifton RP,Otto EA,Han Z,Hildebrandt F

The timing of Timezyme diversification in vertebrates.
PloS one9(12)e112380(2014)
Cazam?a-Catalan D,Besseau L,Falc?n J,Magnanou E

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kank2 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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